DNA2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNA2
Entrez Gene ID	540969
Full Name	DNA replication helicase/nuclease 2
General protein information	Preferred Names DNA replication helicase/nuclease 2 Names DNA replication ATP-dependent helicase/nuclease DNA2 DNA replication ATP-dependent helicase-like homolog DNA replication helicase 2 homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024986762.1	XP_024842530.1	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X3
XM_024986761.1	XP_024842529.1	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X1
XM_010820611.3	XP_010818913.1	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X2
XM_010820611.2	XP_010818913.1	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X1
NM_001192802.2	NP_001179731.2	DNA replication ATP-dependent helicase/nuclease DNA2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DNA2	NP_001006497.1
Bos taurus (cattle)	DNA2	NP_001179731.2
Rattus norvegicus (Norway rat)	Dna2	XP_241671.6
Arabidopsis thaliana (thale cress)	emb2411	NP_001184943.1
Oryza sativa (rice)	Os04g0588200	NP_001053694.2
Canis lupus familiaris (dog)	DNA2	XP_005619489.1
Danio rerio (zebrafish)	dna2	XP_687974.5
Pan troglodytes (chimpanzee)	DNA2	XP_001163213.2
Mus musculus (house mouse)	Dna2	NP_796346.2
Drosophila melanogaster (fruit fly)	CG2990	NP_727386.1
Homo sapiens (human)	DNA2	NP_001073918.2
	DNA2	XP_002808547.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004685	XP_307265.4
Caenorhabditis elegans (roundworm)	dna-2	NP_496515.1
Schizosaccharomyces pombe (fission yeast)	dna2	NP_596499.2
Magnaporthe oryzae (rice blast fungus)	MGG_02491	XP_003709288.1
Xenopus tropicalis (tropical clawed frog)	dna2	XP_002936016.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DNA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa54934	XM_010820611.3 Latest version!	Bos taurus DNA replication helicase/nuclease 2 (DNA2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa231665	XM_024986761.1 Latest version!	Bos taurus DNA replication helicase/nuclease 2 (DNA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa54936	XM_024986762.1 Latest version!	Bos taurus DNA replication helicase/nuclease 2 (DNA2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa44431	NM_001192802.2 Latest version!	Bos taurus DNA replication helicase/nuclease 2 (DNA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa54934	XM_010820611.2	Bos taurus DNA replication helicase/nuclease 2 (DNA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNA2 ( XM_010820611.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818913.1 Bos taurus DNA replication helicase/nuclease 2 (DNA2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa54934
Clone ID Related Accession (Same CDS sequence)	XM_010820611.3 , XM_010820611.2
Accession Version	XM_010820611.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820611.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAG TGGACGAACT GGAGCTGCTA ATGGAGAAGA GTTTCTGGCA GGAGGCTGAG 
CCGTCAGCGG AGCTATTTCA GAAGAAGAAG GTGGAAGCTT CCTTTTCTAA GATAGTTCTG 
AGCAGAGGAA TGGATAACCG GTACCTGGTA TTAGCAGTCG ATATTGTACA GAGTGAAGAA 
GGAAACCATG AAAAGCACCT AATCATCACT GCTTCCCAGT CGCTAGAATA TAAAGAACTG 
TGTATCCTTA GGAATGACTG GTGCTCTGTA CCAGTAGAGC CAGGGGACAT CATTCATTTG 
GAAGGAGACT GCATATCCAA TACTTGGATA ATAGATGAAG ATTTTGGATA TTTGATTCTA 
TATCCCGACA TGCTGATTTC TGGCACAAGC ATAGCCAGTA GTATTCGATG CATGAGAAGA 
GCTGTTCTGA GTGAAACATT CAGGAGCTCT GATCCAGCCA CACGCCAAAT GCTGATTGGT 
ACTGTTCTTC ATGAAGTATT TCAAAAAGCA GTAAGTGATA GTTTTGCCCC AGAAAAGCTA 
CAAGAACTTG CTTCTCAAAC TATTCAAGAA ATAAGGCATC TGAAGGAAAT GTACCGCCTG 
AAATTGAATC AAGATGAAAT AAAACAAGAA GTAGAGGAGT ATCTTCCTTC ATTTTCTAAA 
TGGGCTGGAG ATTTCATGCA TAAACATACT TCAACTGACT TCCCTCAGAT GCAGCTCTCT 
CTGCCAAGTG ATGGTAGTAA CAGTAATTCA ACATGTAATA TTGAAGTTAC AAACTCGCTG 
GATATTGAAG AAAGCATTTG GTCCCCTAGG TTTGGATTGA AGGGCAAAAT CGATGTTACA 
GTTGGTGTGA AAATACATCG AGGATGTAAA ACGAAATATA AGATAATGCC ATTGGAACTT 
AAAACTGGCA AAGAATCAAA TTCTATTGAA CACCGTAGTC AGCTTGTTCT GTATACACTG 
CTAAGCCAAG AGAGGAGGGC TGACCCAGAG GCCGGCCTGC TCCTCTACCT AAAGACTGGC 
CAGATGTACC CTGTGCCTGC CAAACATCTT GATAAAAGAG AATTATTAAG GCTGAGAAAT 
CAGATGGCAT TCTCCTTGTT TCACCGTATT AACAAATCTA CTGGAGAGAA GACAGAGCTT 
GCTCCTTTGC CACAAATAAT TGAGGAACAG CAAACCTGTA AATACTGTTC ACAGATGGGC 
AACTGTGCTC TCTATAGCAG GGCGGTTGAA CAACAGATGG AAGACAGTTC AGTCCCGACC 
AGCATGTGGC CCAAAATAAA AGAAGAAACC CAGCATCTCA AGCCTATACA CTTAGAGTAT 
TTTAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCTCAAT CAAAGGATAA TAAAAGAAAT 
TACCAACATA TATGGTTAAT GCCTGCTTCA GAAATGGAAG AGAGTGGCAG CTGCATTGGG 
AGCCTGATTA GAATAGAACA TGTAAAAACA GTTTGTGATG GACAGTATTT ACATAATTTC 
CAGCGTAAAA ATGGAGCCAT ACCTATCACA AATCTAATGG CAGGTGACAG AATTATTTTA 
AGTGGAGAAG AGAGAACATT GTTTGCCTTG TCTAGGGGAT ACGTGAAGGA GATTAACTCA 
ACAACAGTAA CCTGTTCATT AGACAGGAAC TTGTCAGGAC TCCCAGAATC AACTTTGTTC 
AGATTGGACC AGGAAGAAAA AAATTGTGAT ATAGATACCC CATTAGGAAA TCTTTCTAAA 
CTGATGGAAA ATACTCGTGC CAGCCAAAAA CTTCGAGATT TAATTATTGA CTTTCGTGAA 
CCACAGTTTA TATCCTACCT CAGTTCTGTT CTTCCACATG AGGCAAAGGA CACAGTTGCC 
TGCATTCTGA AAGGTTTGAA TAAGCCTCAG AGGCAAGCCA TGAAAAAGGT ACTTCTTTCA 
AAAGACTACA CCCTCATCGT GGGCATGCCG GGAACAGGAA AAACAACTAC AATATGTACT 
CTTGTAAGAA TTCTCTATGC CTGTGGTTTT AGTGTTTTGT TGACCAGCTA TACACACTCT 
GCTGTTGACA ATATTCTTTT GAAGTTAGCA AAGTTTAAAA TTGGATTTTT GCGCTTGGGC 
CAGATTCAGA AGGTACATCC AGATATCCAA AAATTTACAG AGGAGGAAAT TTGCAGATCA 
AAGTCCATTA AATCTTTAGC TCTTCTAGAA GAACTCTACA ACAGTCAACT TATAGTTGCA 
ACAACATGTA TGGGAATAAA CCATCCCATA TTTTCTCGTA AAACTTTTGA TTTTTGTATA 
GTGGATGAAG CCTCTCAAAT TAGCCAGCCT GTTTGCCTAG GGCCACTTTT TTTTTCACGG 
AGATTTGTGT TGGTGGGGGA CCATCAGCAG CTTCCTCCCC TGGTGTTAAA CCGTGAAGCA 
AGAGCTCTTG GCATGAGTGA AAGCTTATTC AAGAGGCTGG AGCAGAATAA GAATGCAGTT 
GTACAGTTAA CTGTGCAATA CAGAATGAAC AGTAAAATTA TGTCCTTAAG TAATAAACTG 
ACGTACGAAG GAAAGCTGGA GTGTGGATCA GATAAAGTGG CCAATGCAGT GATAAACCTG 
CCTAACTTTA AAGATGTAAA GCTGGAACTG GAGTTTTATG CTGATTATTC AGAAAATCCT 
TGGCTGATTG CAGCATTCGA GCCAAACAAT CCTGTCTGTT TTCTTAATAC ACACAAGGTT 
CCAGCACCAG AACAAGTTGA AAAGGGAGGT GTCAGTAATA TAATGGAAGC CAAACTCGTT 
GTTTTCCTGA CGTCAGTTTT TATTAAGGCT GGTTGTAAAC CCTCTGATAT TGGTATCATT 
GCACCATACA GGCAGCAGTT AAAGGTCATC AGTGACTTAT TGGCACAGTC TTCTGTTGGG 
ATGGTCGAAG TTAATACAGT TGACAGCTTG GTGAATTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818913.1
CDS	160..3081
Translation	MERVDELELLMEKSFWQEAEPSAELFQKKKVEASFSKIVLSRGMDNRYLVLAVDIVQSEEGNHEKHLIITASQSLEYKELCILRNDWCSVPVEPGDIIHLEGDCISNTWIIDEDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRSSDPATRQMLIGTVLHEVFQKAVSDSFAPEKLQELASQTIQEIRHLKEMYRLKLNQDEIKQEVEEYLPSFSKWAGDFMHKHTSTDFPQMQLSLPSDGSNSNSTCNIEVTNSLDIEESIWSPRFGLKGKIDVTVGVKIHRGCKTKYKIMPLELKTGKESNSIEHRSQLVLYTLLSQERRADPEAGLLLYLKTGQMYPVPAKHLDKRELLRLRNQMAFSLFHRINKSTGEKTELAPLPQIIEEQQTCKYCSQMGNCALYSRAVEQQMEDSSVPTSMWPKIKEETQHLKPIHLEYFSLWCLMLTLESQSKDNKRNYQHIWLMPASEMEESGSCIGSLIRIEHVKTVCDGQYLHNFQRKNGAIPITNLMAGDRIILSGEERTLFALSRGYVKEINSTTVTCSLDRNLSGLPESTLFRLDQEEKNCDIDTPLGNLSKLMENTRASQKLRDLIIDFREPQFISYLSSVLPHEAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPDIQKFTEEEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKTFDFCIVDEASQISQPVCLGPLFFSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKNAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLPNFKDVKLELEFYADYSENPWLIAAFEPNNPVCFLNTHKVPAPEQVEKGGVSNIMEAKLVVFLTSVFIKAGCKPSDIGIIAPYRQQLKVISDLLAQSSVGMVEVNTVDSLVNC

Target ORF information:

RefSeq Version	XM_010820611.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA replication helicase/nuclease 2 (DNA2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820611.3

ORF Insert Sequence:

ATGGAGCGAG TGGACGAACT GGAGCTGCTA ATGGAGAAGA GTTTCTGGCA GGAGGCTGAG 
CCGTCAGCGG AGCTATTTCA GAAGAAGAAG GTGGAAGCTT CCTTTTCTAA GATAGTTCTG 
AGCAGAGGAA TGGATAACCG GTACCTGGTA TTAGCAGTCG ATATTGTACA GAGTGAAGAA 
GGAAACCATG AAAAGCACCT AATCATCACT GCTTCCCAGT CGCTAGAATA TAAAGAACTG 
TGTATCCTTA GGAATGACTG GTGCTCTGTA CCAGTAGAGC CAGGGGACAT CATTCATTTG 
GAAGGAGACT GCATATCCAA TACTTGGATA ATAGATGAAG ATTTTGGATA TTTGATTCTA 
TATCCCGACA TGCTGATTTC TGGCACAAGC ATAGCCAGTA GTATTCGATG CATGAGAAGA 
GCTGTTCTGA GTGAAACATT CAGGAGCTCT GATCCAGCCA CACGCCAAAT GCTGATTGGT 
ACTGTTCTTC ATGAAGTATT TCAAAAAGCA GTAAGTGATA GTTTTGCCCC AGAAAAGCTA 
CAAGAACTTG CTTCTCAAAC TATTCAAGAA ATAAGGCATC TGAAGGAAAT GTACCGCCTG 
AAATTGAATC AAGATGAAAT AAAACAAGAA GTAGAGGAGT ATCTTCCTTC ATTTTCTAAA 
TGGGCTGGAG ATTTCATGCA TAAACATACT TCAACTGACT TCCCTCAGAT GCAGCTCTCT 
CTGCCAAGTG ATGGTAGTAA CAGTAATTCA ACATGTAATA TTGAAGTTAC AAACTCGCTG 
GATATTGAAG AAAGCATTTG GTCCCCTAGG TTTGGATTGA AGGGCAAAAT CGATGTTACA 
GTTGGTGTGA AAATACATCG AGGATGTAAA ACGAAATATA AGATAATGCC ATTGGAACTT 
AAAACTGGCA AAGAATCAAA TTCTATTGAA CACCGTAGTC AGCTTGTTCT GTATACACTG 
CTAAGCCAAG AGAGGAGGGC TGACCCAGAG GCCGGCCTGC TCCTCTACCT AAAGACTGGC 
CAGATGTACC CTGTGCCTGC CAAACATCTT GATAAAAGAG AATTATTAAG GCTGAGAAAT 
CAGATGGCAT TCTCCTTGTT TCACCGTATT AACAAATCTA CTGGAGAGAA GACAGAGCTT 
GCTCCTTTGC CACAAATAAT TGAGGAACAG CAAACCTGTA AATACTGTTC ACAGATGGGC 
AACTGTGCTC TCTATAGCAG GGCGGTTGAA CAACAGATGG AAGACAGTTC AGTCCCGACC 
AGCATGTGGC CCAAAATAAA AGAAGAAACC CAGCATCTCA AGCCTATACA CTTAGAGTAT 
TTTAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCTCAAT CAAAGGATAA TAAAAGAAAT 
TACCAACATA TATGGTTAAT GCCTGCTTCA GAAATGGAAG AGAGTGGCAG CTGCATTGGG 
AGCCTGATTA GAATAGAACA TGTAAAAACA GTTTGTGATG GACAGTATTT ACATAATTTC 
CAGCGTAAAA ATGGAGCCAT ACCTATCACA AATCTAATGG CAGGTGACAG AATTATTTTA 
AGTGGAGAAG AGAGAACATT GTTTGCCTTG TCTAGGGGAT ACGTGAAGGA GATTAACTCA 
ACAACAGTAA CCTGTTCATT AGACAGGAAC TTGTCAGGAC TCCCAGAATC AACTTTGTTC 
AGATTGGACC AGGAAGAAAA AAATTGTGAT ATAGATACCC CATTAGGAAA TCTTTCTAAA 
CTGATGGAAA ATACTCGTGC CAGCCAAAAA CTTCGAGATT TAATTATTGA CTTTCGTGAA 
CCACAGTTTA TATCCTACCT CAGTTCTGTT CTTCCACATG AGGCAAAGGA CACAGTTGCC 
TGCATTCTGA AAGGTTTGAA TAAGCCTCAG AGGCAAGCCA TGAAAAAGGT ACTTCTTTCA 
AAAGACTACA CCCTCATCGT GGGCATGCCG GGAACAGGAA AAACAACTAC AATATGTACT 
CTTGTAAGAA TTCTCTATGC CTGTGGTTTT AGTGTTTTGT TGACCAGCTA TACACACTCT 
GCTGTTGACA ATATTCTTTT GAAGTTAGCA AAGTTTAAAA TTGGATTTTT GCGCTTGGGC 
CAGATTCAGA AGGTACATCC AGATATCCAA AAATTTACAG AGGAGGAAAT TTGCAGATCA 
AAGTCCATTA AATCTTTAGC TCTTCTAGAA GAACTCTACA ACAGTCAACT TATAGTTGCA 
ACAACATGTA TGGGAATAAA CCATCCCATA TTTTCTCGTA AAACTTTTGA TTTTTGTATA 
GTGGATGAAG CCTCTCAAAT TAGCCAGCCT GTTTGCCTAG GGCCACTTTT TTTTTCACGG 
AGATTTGTGT TGGTGGGGGA CCATCAGCAG CTTCCTCCCC TGGTGTTAAA CCGTGAAGCA 
AGAGCTCTTG GCATGAGTGA AAGCTTATTC AAGAGGCTGG AGCAGAATAA GAATGCAGTT 
GTACAGTTAA CTGTGCAATA CAGAATGAAC AGTAAAATTA TGTCCTTAAG TAATAAACTG 
ACGTACGAAG GAAAGCTGGA GTGTGGATCA GATAAAGTGG CCAATGCAGT GATAAACCTG 
CCTAACTTTA AAGATGTAAA GCTGGAACTG GAGTTTTATG CTGATTATTC AGAAAATCCT 
TGGCTGATTG CAGCATTCGA GCCAAACAAT CCTGTCTGTT TTCTTAATAC ACACAAGGTT 
CCAGCACCAG AACAAGTTGA AAAGGGAGGT GTCAGTAATA TAATGGAAGC CAAACTCGTT 
GTTTTCCTGA CGTCAGTTTT TATTAAGGCT GGTTGTAAAC CCTCTGATAT TGGTATCATT 
GCACCATACA GGCAGCAGTT AAAGGTCATC AGTGACTTAT TGGCACAGTC TTCTGTTGGG 
ATGGTCGAAG TTAATACAGT TGACAGCTTG GTGAATTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNA2 ( XM_010820611.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818913.1 Bos taurus DNA replication helicase/nuclease 2 (DNA2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa54934
Clone ID Related Accession (Same CDS sequence)	XM_010820611.3 , XM_010820611.2
Accession Version	XM_010820611.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820611.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAG TGGACGAACT GGAGCTGCTA ATGGAGAAGA GTTTCTGGCA GGAGGCTGAG 
CCGTCAGCGG AGCTATTTCA GAAGAAGAAG GTGGAAGCTT CCTTTTCTAA GATAGTTCTG 
AGCAGAGGAA TGGATAACCG GTACCTGGTA TTAGCAGTCG ATATTGTACA GAGTGAAGAA 
GGAAACCATG AAAAGCACCT AATCATCACT GCTTCCCAGT CGCTAGAATA TAAAGAACTG 
TGTATCCTTA GGAATGACTG GTGCTCTGTA CCAGTAGAGC CAGGGGACAT CATTCATTTG 
GAAGGAGACT GCATATCCAA TACTTGGATA ATAGATGAAG ATTTTGGATA TTTGATTCTA 
TATCCCGACA TGCTGATTTC TGGCACAAGC ATAGCCAGTA GTATTCGATG CATGAGAAGA 
GCTGTTCTGA GTGAAACATT CAGGAGCTCT GATCCAGCCA CACGCCAAAT GCTGATTGGT 
ACTGTTCTTC ATGAAGTATT TCAAAAAGCA GTAAGTGATA GTTTTGCCCC AGAAAAGCTA 
CAAGAACTTG CTTCTCAAAC TATTCAAGAA ATAAGGCATC TGAAGGAAAT GTACCGCCTG 
AAATTGAATC AAGATGAAAT AAAACAAGAA GTAGAGGAGT ATCTTCCTTC ATTTTCTAAA 
TGGGCTGGAG ATTTCATGCA TAAACATACT TCAACTGACT TCCCTCAGAT GCAGCTCTCT 
CTGCCAAGTG ATGGTAGTAA CAGTAATTCA ACATGTAATA TTGAAGTTAC AAACTCGCTG 
GATATTGAAG AAAGCATTTG GTCCCCTAGG TTTGGATTGA AGGGCAAAAT CGATGTTACA 
GTTGGTGTGA AAATACATCG AGGATGTAAA ACGAAATATA AGATAATGCC ATTGGAACTT 
AAAACTGGCA AAGAATCAAA TTCTATTGAA CACCGTAGTC AGCTTGTTCT GTATACACTG 
CTAAGCCAAG AGAGGAGGGC TGACCCAGAG GCCGGCCTGC TCCTCTACCT AAAGACTGGC 
CAGATGTACC CTGTGCCTGC CAAACATCTT GATAAAAGAG AATTATTAAG GCTGAGAAAT 
CAGATGGCAT TCTCCTTGTT TCACCGTATT AACAAATCTA CTGGAGAGAA GACAGAGCTT 
GCTCCTTTGC CACAAATAAT TGAGGAACAG CAAACCTGTA AATACTGTTC ACAGATGGGC 
AACTGTGCTC TCTATAGCAG GGCGGTTGAA CAACAGATGG AAGACAGTTC AGTCCCGACC 
AGCATGTGGC CCAAAATAAA AGAAGAAACC CAGCATCTCA AGCCTATACA CTTAGAGTAT 
TTTAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCTCAAT CAAAGGATAA TAAAAGAAAT 
TACCAACATA TATGGTTAAT GCCTGCTTCA GAAATGGAAG AGAGTGGCAG CTGCATTGGG 
AGCCTGATTA GAATAGAACA TGTAAAAACA GTTTGTGATG GACAGTATTT ACATAATTTC 
CAGCGTAAAA ATGGAGCCAT ACCTATCACA AATCTAATGG CAGGTGACAG AATTATTTTA 
AGTGGAGAAG AGAGAACATT GTTTGCCTTG TCTAGGGGAT ACGTGAAGGA GATTAACTCA 
ACAACAGTAA CCTGTTCATT AGACAGGAAC TTGTCAGGAC TCCCAGAATC AACTTTGTTC 
AGATTGGACC AGGAAGAAAA AAATTGTGAT ATAGATACCC CATTAGGAAA TCTTTCTAAA 
CTGATGGAAA ATACTCGTGC CAGCCAAAAA CTTCGAGATT TAATTATTGA CTTTCGTGAA 
CCACAGTTTA TATCCTACCT CAGTTCTGTT CTTCCACATG AGGCAAAGGA CACAGTTGCC 
TGCATTCTGA AAGGTTTGAA TAAGCCTCAG AGGCAAGCCA TGAAAAAGGT ACTTCTTTCA 
AAAGACTACA CCCTCATCGT GGGCATGCCG GGAACAGGAA AAACAACTAC AATATGTACT 
CTTGTAAGAA TTCTCTATGC CTGTGGTTTT AGTGTTTTGT TGACCAGCTA TACACACTCT 
GCTGTTGACA ATATTCTTTT GAAGTTAGCA AAGTTTAAAA TTGGATTTTT GCGCTTGGGC 
CAGATTCAGA AGGTACATCC AGATATCCAA AAATTTACAG AGGAGGAAAT TTGCAGATCA 
AAGTCCATTA AATCTTTAGC TCTTCTAGAA GAACTCTACA ACAGTCAACT TATAGTTGCA 
ACAACATGTA TGGGAATAAA CCATCCCATA TTTTCTCGTA AAACTTTTGA TTTTTGTATA 
GTGGATGAAG CCTCTCAAAT TAGCCAGCCT GTTTGCCTAG GGCCACTTTT TTTTTCACGG 
AGATTTGTGT TGGTGGGGGA CCATCAGCAG CTTCCTCCCC TGGTGTTAAA CCGTGAAGCA 
AGAGCTCTTG GCATGAGTGA AAGCTTATTC AAGAGGCTGG AGCAGAATAA GAATGCAGTT 
GTACAGTTAA CTGTGCAATA CAGAATGAAC AGTAAAATTA TGTCCTTAAG TAATAAACTG 
ACGTACGAAG GAAAGCTGGA GTGTGGATCA GATAAAGTGG CCAATGCAGT GATAAACCTG 
CCTAACTTTA AAGATGTAAA GCTGGAACTG GAGTTTTATG CTGATTATTC AGAAAATCCT 
TGGCTGATTG CAGCATTCGA GCCAAACAAT CCTGTCTGTT TTCTTAATAC ACACAAGGTT 
CCAGCACCAG AACAAGTTGA AAAGGGAGGT GTCAGTAATA TAATGGAAGC CAAACTCGTT 
GTTTTCCTGA CGTCAGTTTT TATTAAGGCT GGTTGTAAAC CCTCTGATAT TGGTATCATT 
GCACCATACA GGCAGCAGTT AAAGGTCATC AGTGACTTAT TGGCACAGTC TTCTGTTGGG 
ATGGTCGAAG TTAATACAGT TGACAGCTTG GTGAATTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818913.1
CDS	127..3048
Translation	MERVDELELLMEKSFWQEAEPSAELFQKKKVEASFSKIVLSRGMDNRYLVLAVDIVQSEEGNHEKHLIITASQSLEYKELCILRNDWCSVPVEPGDIIHLEGDCISNTWIIDEDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRSSDPATRQMLIGTVLHEVFQKAVSDSFAPEKLQELASQTIQEIRHLKEMYRLKLNQDEIKQEVEEYLPSFSKWAGDFMHKHTSTDFPQMQLSLPSDGSNSNSTCNIEVTNSLDIEESIWSPRFGLKGKIDVTVGVKIHRGCKTKYKIMPLELKTGKESNSIEHRSQLVLYTLLSQERRADPEAGLLLYLKTGQMYPVPAKHLDKRELLRLRNQMAFSLFHRINKSTGEKTELAPLPQIIEEQQTCKYCSQMGNCALYSRAVEQQMEDSSVPTSMWPKIKEETQHLKPIHLEYFSLWCLMLTLESQSKDNKRNYQHIWLMPASEMEESGSCIGSLIRIEHVKTVCDGQYLHNFQRKNGAIPITNLMAGDRIILSGEERTLFALSRGYVKEINSTTVTCSLDRNLSGLPESTLFRLDQEEKNCDIDTPLGNLSKLMENTRASQKLRDLIIDFREPQFISYLSSVLPHEAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPDIQKFTEEEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKTFDFCIVDEASQISQPVCLGPLFFSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKNAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLPNFKDVKLELEFYADYSENPWLIAAFEPNNPVCFLNTHKVPAPEQVEKGGVSNIMEAKLVVFLTSVFIKAGCKPSDIGIIAPYRQQLKVISDLLAQSSVGMVEVNTVDSLVNC

Target ORF information:

RefSeq Version	XM_010820611.2
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA replication helicase/nuclease 2 (DNA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820611.2

ORF Insert Sequence:

ATGGAGCGAG TGGACGAACT GGAGCTGCTA ATGGAGAAGA GTTTCTGGCA GGAGGCTGAG 
CCGTCAGCGG AGCTATTTCA GAAGAAGAAG GTGGAAGCTT CCTTTTCTAA GATAGTTCTG 
AGCAGAGGAA TGGATAACCG GTACCTGGTA TTAGCAGTCG ATATTGTACA GAGTGAAGAA 
GGAAACCATG AAAAGCACCT AATCATCACT GCTTCCCAGT CGCTAGAATA TAAAGAACTG 
TGTATCCTTA GGAATGACTG GTGCTCTGTA CCAGTAGAGC CAGGGGACAT CATTCATTTG 
GAAGGAGACT GCATATCCAA TACTTGGATA ATAGATGAAG ATTTTGGATA TTTGATTCTA 
TATCCCGACA TGCTGATTTC TGGCACAAGC ATAGCCAGTA GTATTCGATG CATGAGAAGA 
GCTGTTCTGA GTGAAACATT CAGGAGCTCT GATCCAGCCA CACGCCAAAT GCTGATTGGT 
ACTGTTCTTC ATGAAGTATT TCAAAAAGCA GTAAGTGATA GTTTTGCCCC AGAAAAGCTA 
CAAGAACTTG CTTCTCAAAC TATTCAAGAA ATAAGGCATC TGAAGGAAAT GTACCGCCTG 
AAATTGAATC AAGATGAAAT AAAACAAGAA GTAGAGGAGT ATCTTCCTTC ATTTTCTAAA 
TGGGCTGGAG ATTTCATGCA TAAACATACT TCAACTGACT TCCCTCAGAT GCAGCTCTCT 
CTGCCAAGTG ATGGTAGTAA CAGTAATTCA ACATGTAATA TTGAAGTTAC AAACTCGCTG 
GATATTGAAG AAAGCATTTG GTCCCCTAGG TTTGGATTGA AGGGCAAAAT CGATGTTACA 
GTTGGTGTGA AAATACATCG AGGATGTAAA ACGAAATATA AGATAATGCC ATTGGAACTT 
AAAACTGGCA AAGAATCAAA TTCTATTGAA CACCGTAGTC AGCTTGTTCT GTATACACTG 
CTAAGCCAAG AGAGGAGGGC TGACCCAGAG GCCGGCCTGC TCCTCTACCT AAAGACTGGC 
CAGATGTACC CTGTGCCTGC CAAACATCTT GATAAAAGAG AATTATTAAG GCTGAGAAAT 
CAGATGGCAT TCTCCTTGTT TCACCGTATT AACAAATCTA CTGGAGAGAA GACAGAGCTT 
GCTCCTTTGC CACAAATAAT TGAGGAACAG CAAACCTGTA AATACTGTTC ACAGATGGGC 
AACTGTGCTC TCTATAGCAG GGCGGTTGAA CAACAGATGG AAGACAGTTC AGTCCCGACC 
AGCATGTGGC CCAAAATAAA AGAAGAAACC CAGCATCTCA AGCCTATACA CTTAGAGTAT 
TTTAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCTCAAT CAAAGGATAA TAAAAGAAAT 
TACCAACATA TATGGTTAAT GCCTGCTTCA GAAATGGAAG AGAGTGGCAG CTGCATTGGG 
AGCCTGATTA GAATAGAACA TGTAAAAACA GTTTGTGATG GACAGTATTT ACATAATTTC 
CAGCGTAAAA ATGGAGCCAT ACCTATCACA AATCTAATGG CAGGTGACAG AATTATTTTA 
AGTGGAGAAG AGAGAACATT GTTTGCCTTG TCTAGGGGAT ACGTGAAGGA GATTAACTCA 
ACAACAGTAA CCTGTTCATT AGACAGGAAC TTGTCAGGAC TCCCAGAATC AACTTTGTTC 
AGATTGGACC AGGAAGAAAA AAATTGTGAT ATAGATACCC CATTAGGAAA TCTTTCTAAA 
CTGATGGAAA ATACTCGTGC CAGCCAAAAA CTTCGAGATT TAATTATTGA CTTTCGTGAA 
CCACAGTTTA TATCCTACCT CAGTTCTGTT CTTCCACATG AGGCAAAGGA CACAGTTGCC 
TGCATTCTGA AAGGTTTGAA TAAGCCTCAG AGGCAAGCCA TGAAAAAGGT ACTTCTTTCA 
AAAGACTACA CCCTCATCGT GGGCATGCCG GGAACAGGAA AAACAACTAC AATATGTACT 
CTTGTAAGAA TTCTCTATGC CTGTGGTTTT AGTGTTTTGT TGACCAGCTA TACACACTCT 
GCTGTTGACA ATATTCTTTT GAAGTTAGCA AAGTTTAAAA TTGGATTTTT GCGCTTGGGC 
CAGATTCAGA AGGTACATCC AGATATCCAA AAATTTACAG AGGAGGAAAT TTGCAGATCA 
AAGTCCATTA AATCTTTAGC TCTTCTAGAA GAACTCTACA ACAGTCAACT TATAGTTGCA 
ACAACATGTA TGGGAATAAA CCATCCCATA TTTTCTCGTA AAACTTTTGA TTTTTGTATA 
GTGGATGAAG CCTCTCAAAT TAGCCAGCCT GTTTGCCTAG GGCCACTTTT TTTTTCACGG 
AGATTTGTGT TGGTGGGGGA CCATCAGCAG CTTCCTCCCC TGGTGTTAAA CCGTGAAGCA 
AGAGCTCTTG GCATGAGTGA AAGCTTATTC AAGAGGCTGG AGCAGAATAA GAATGCAGTT 
GTACAGTTAA CTGTGCAATA CAGAATGAAC AGTAAAATTA TGTCCTTAAG TAATAAACTG 
ACGTACGAAG GAAAGCTGGA GTGTGGATCA GATAAAGTGG CCAATGCAGT GATAAACCTG 
CCTAACTTTA AAGATGTAAA GCTGGAACTG GAGTTTTATG CTGATTATTC AGAAAATCCT 
TGGCTGATTG CAGCATTCGA GCCAAACAAT CCTGTCTGTT TTCTTAATAC ACACAAGGTT 
CCAGCACCAG AACAAGTTGA AAAGGGAGGT GTCAGTAATA TAATGGAAGC CAAACTCGTT 
GTTTTCCTGA CGTCAGTTTT TATTAAGGCT GGTTGTAAAC CCTCTGATAT TGGTATCATT 
GCACCATACA GGCAGCAGTT AAAGGTCATC AGTGACTTAT TGGCACAGTC TTCTGTTGGG 
ATGGTCGAAG TTAATACAGT TGACAGCTTG GTGAATTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNA2 ( XM_024986761.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842529.1 Bos taurus DNA replication helicase/nuclease 2 (DNA2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa231665
Clone ID Related Accession (Same CDS sequence)	XM_024986761.1
Accession Version	XM_024986761.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAG TGGACGAACT GGAGCTGCTA ATGGAGAAGA GTTTCTGGCA GGAGGCTGAG 
CCGTCAGCGG AGCTATTTCA GAAGAAGAAG GTGGAAGCTT CCTTTTCTAA GATAGTTCTG 
AGCAGAGGAA TGGATAACCG GTACCTGGTA TTAGCAGTCG ATATTGTACA GAGTGAAGAA 
GGAAACCATG AAAAGCACCT AATCATCACT GCTTCCCAGT CGCTAGAATA TAAAGAACTG 
TGTATCCTTA GGAATGACTG GTGCTCTGTA CCAGTAGAGC CAGGGGACAT CATTCATTTG 
GAAGGAGACT GCATATCCAA TACTTGGATA ATAGATGAAG ATTTTGGATA TTTGATTCTA 
TATCCCGACA TGCTGATTTC TGGCACAAGC ATAGCCAGTA GTATTCGATG CATGAGAAGA 
GCTGTTCTGA GTGAAACATT CAGGAGCTCT GATCCAGCCA CACGCCAAAT GCTGATTGGT 
ACTGTTCTTC ATGAAGTATT TCAAAAAGCA GTAAGTGATA GTTTTGCCCC AGAAAAGCTA 
CAAGAACTTG CTTCTCAAAC TATTCAAGAA ATAAGGCATC TGAAGGAAAT GTACCGCCTG 
AAATTGAATC AAGATGAAAT AAAACAAGAA GTAGAGGAGT ATCTTCCTTC ATTTTCTAAA 
TGGGCTGGAG ATTTCATGCA TAAACATACT TCAACTGACT TCCCTCAGAT GCAGCTCTCT 
CTGCCAAGTG ATGGTAGTAA CAGTAATTCA ACATGTAATA TTGAAGTTAC AAACTCGCTG 
GATATTGAAG AAAGCATTTG GTCCCCTAGG TTTGGATTGA AGGGCAAAAT CGATGTTACA 
GTTGGTGTGA AAATACATCG AGGATGTAAA ACGAAATATA AGATAATGCC ATTGGAACTT 
AAAACTGGCA AAGAATCAAA TTCTATTGAA CACCGTAGTC AGCTTGTTCT GTATACACTG 
CTAAGCCAAG AGAGGAGGGC TGACCCAGAG GCCGGCCTGC TCCTCTACCT AAAGACTGGC 
CAGATGTACC CTGTGCCTGC CAAACATCTT GATAAAAGAG AATTATTAAG GCTGAGAAAT 
CAGATGGCAT TCTCCTTGTT TCACCGTATT AACAAATCTA CTGGAGAGAA GACAGAGCTT 
GCTCCTTTGC CACAAATAAT TGAGGAACAG CAAACCTGTA AATACTGTTC ACAGATGGGC 
AACTGTGCTC TCTATAGCAG GGCGGTTGAA CAACAGATGG AAGACAGTTC AGTCCCGACC 
AGCATGTGGC CCAAAATAAA AGAAGAAACC CAGCATCTCA AGCCTATACA CTTAGAGTAT 
TTTAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCTCAAT CAAAGGATAA TAAAAGAAAT 
TACCAACATA TATGGTTAAT GCCTGCTTCA GAAATGAACT TGTCAGGACT CCCAGAATCA 
ACTTTGTTCA GATTGGACCA GGAAGAAAAA AATTGTGATA TAGATACCCC ATTAGGAAAT 
CTTTCTAAAC TGATGGAAAA TACTCGTGCC AGCCAAAAAC TTCGAGATTT AATTATTGAC 
TTTCGTGAAC CACAGTTTAT ATCCTACCTC AGTTCTGTTC TTCCACATGA GGCAAAGGAC 
ACAGTTGCCT GCATTCTGAA AGGTTTGAAT AAGCCTCAGA GGCAAGCCAT GAAAAAGGTA 
CTTCTTTCAA AAGACTACAC CCTCATCGTG GGCATGCCGG GAACAGGAAA AACAACTACA 
ATATGTACTC TTGTAAGAAT TCTCTATGCC TGTGGTTTTA GTGTTTTGTT GACCAGCTAT 
ACACACTCTG CTGTTGACAA TATTCTTTTG AAGTTAGCAA AGTTTAAAAT TGGATTTTTG 
CGCTTGGGCC AGATTCAGAA GGTACATCCA GATATCCAAA AATTTACAGA GGAGGAAATT 
TGCAGATCAA AGTCCATTAA ATCTTTAGCT CTTCTAGAAG AACTCTACAA CAGTCAACTT 
ATAGTTGCAA CAACATGTAT GGGAATAAAC CATCCCATAT TTTCTCGTAA AACTTTTGAT 
TTTTGTATAG TGGATGAAGC CTCTCAAATT AGCCAGCCTG TTTGCCTAGG GCCACTTTTT 
TTTTCACGGA GATTTGTGTT GGTGGGGGAC CATCAGCAGC TTCCTCCCCT GGTGTTAAAC 
CGTGAAGCAA GAGCTCTTGG CATGAGTGAA AGCTTATTCA AGAGGCTGGA GCAGAATAAG 
AATGCAGTTG TACAGTTAAC TGTGCAATAC AGAATGAACA GTAAAATTAT GTCCTTAAGT 
AATAAACTGA CGTACGAAGG AAAGCTGGAG TGTGGATCAG ATAAAGTGGC CAATGCAGTG 
ATAAACCTGC CTAACTTTAA AGATGTAAAG CTGGAACTGG AGTTTTATGC TGATTATTCA 
GAAAATCCTT GGCTGATTGC AGCATTCGAG CCAAACAATC CTGTCTGTTT TCTTAATACA 
CACAAGGTTC CAGCACCAGA ACAAGTTGAA AAGGGAGGTG TCAGTAATAT AATGGAAGCC 
AAACTCGTTG TTTTCCTGAC GTCAGTTTTT ATTAAGGCTG GTTGTAAACC CTCTGATATT 
GGTATCATTG CACCATACAG GCAGCAGTTA AAGGTCATCA GTGACTTATT GGCACAGTCT 
TCTGTTGGGA TGGTCGAAGT TAATACAGTT GACAGGTACC AAGGAAGAGA CAAAAGTATC 
GTTGTAGTAT CTTTTGTTAG AAGCAATGAA GATGGAACTC TTGGTGAATT GTTGAAAGAT 
TGGCGACGTC TTAATGTCGC TATAACCAGA GCCAAACATA AGCTGATTCT CCTGGGATGT 
GTGCCCTCAC TAAGCCGTTA CCCTCCTTTG AGGAAGCTAC TTAATCATCT CAACTCAGAA 
AAATTAATCA TTGATCTTCC ATCAGGAGAA CATGAAAGCC TTTTTCATCT ATTGGGTGAT 
TTCCAAAGAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842529.1
CDS	163..3117
Translation	MERVDELELLMEKSFWQEAEPSAELFQKKKVEASFSKIVLSRGMDNRYLVLAVDIVQSEEGNHEKHLIITASQSLEYKELCILRNDWCSVPVEPGDIIHLEGDCISNTWIIDEDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRSSDPATRQMLIGTVLHEVFQKAVSDSFAPEKLQELASQTIQEIRHLKEMYRLKLNQDEIKQEVEEYLPSFSKWAGDFMHKHTSTDFPQMQLSLPSDGSNSNSTCNIEVTNSLDIEESIWSPRFGLKGKIDVTVGVKIHRGCKTKYKIMPLELKTGKESNSIEHRSQLVLYTLLSQERRADPEAGLLLYLKTGQMYPVPAKHLDKRELLRLRNQMAFSLFHRINKSTGEKTELAPLPQIIEEQQTCKYCSQMGNCALYSRAVEQQMEDSSVPTSMWPKIKEETQHLKPIHLEYFSLWCLMLTLESQSKDNKRNYQHIWLMPASEMNLSGLPESTLFRLDQEEKNCDIDTPLGNLSKLMENTRASQKLRDLIIDFREPQFISYLSSVLPHEAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPDIQKFTEEEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKTFDFCIVDEASQISQPVCLGPLFFSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKNAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLPNFKDVKLELEFYADYSENPWLIAAFEPNNPVCFLNTHKVPAPEQVEKGGVSNIMEAKLVVFLTSVFIKAGCKPSDIGIIAPYRQQLKVISDLLAQSSVGMVEVNTVDRYQGRDKSIVVVSFVRSNEDGTLGELLKDWRRLNVAITRAKHKLILLGCVPSLSRYPPLRKLLNHLNSEKLIIDLPSGEHESLFHLLGDFQRK

Target ORF information:

RefSeq Version	XM_024986761.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA replication helicase/nuclease 2 (DNA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986761.1

ORF Insert Sequence:

ATGGAGCGAG TGGACGAACT GGAGCTGCTA ATGGAGAAGA GTTTCTGGCA GGAGGCTGAG 
CCGTCAGCGG AGCTATTTCA GAAGAAGAAG GTGGAAGCTT CCTTTTCTAA GATAGTTCTG 
AGCAGAGGAA TGGATAACCG GTACCTGGTA TTAGCAGTCG ATATTGTACA GAGTGAAGAA 
GGAAACCATG AAAAGCACCT AATCATCACT GCTTCCCAGT CGCTAGAATA TAAAGAACTG 
TGTATCCTTA GGAATGACTG GTGCTCTGTA CCAGTAGAGC CAGGGGACAT CATTCATTTG 
GAAGGAGACT GCATATCCAA TACTTGGATA ATAGATGAAG ATTTTGGATA TTTGATTCTA 
TATCCCGACA TGCTGATTTC TGGCACAAGC ATAGCCAGTA GTATTCGATG CATGAGAAGA 
GCTGTTCTGA GTGAAACATT CAGGAGCTCT GATCCAGCCA CACGCCAAAT GCTGATTGGT 
ACTGTTCTTC ATGAAGTATT TCAAAAAGCA GTAAGTGATA GTTTTGCCCC AGAAAAGCTA 
CAAGAACTTG CTTCTCAAAC TATTCAAGAA ATAAGGCATC TGAAGGAAAT GTACCGCCTG 
AAATTGAATC AAGATGAAAT AAAACAAGAA GTAGAGGAGT ATCTTCCTTC ATTTTCTAAA 
TGGGCTGGAG ATTTCATGCA TAAACATACT TCAACTGACT TCCCTCAGAT GCAGCTCTCT 
CTGCCAAGTG ATGGTAGTAA CAGTAATTCA ACATGTAATA TTGAAGTTAC AAACTCGCTG 
GATATTGAAG AAAGCATTTG GTCCCCTAGG TTTGGATTGA AGGGCAAAAT CGATGTTACA 
GTTGGTGTGA AAATACATCG AGGATGTAAA ACGAAATATA AGATAATGCC ATTGGAACTT 
AAAACTGGCA AAGAATCAAA TTCTATTGAA CACCGTAGTC AGCTTGTTCT GTATACACTG 
CTAAGCCAAG AGAGGAGGGC TGACCCAGAG GCCGGCCTGC TCCTCTACCT AAAGACTGGC 
CAGATGTACC CTGTGCCTGC CAAACATCTT GATAAAAGAG AATTATTAAG GCTGAGAAAT 
CAGATGGCAT TCTCCTTGTT TCACCGTATT AACAAATCTA CTGGAGAGAA GACAGAGCTT 
GCTCCTTTGC CACAAATAAT TGAGGAACAG CAAACCTGTA AATACTGTTC ACAGATGGGC 
AACTGTGCTC TCTATAGCAG GGCGGTTGAA CAACAGATGG AAGACAGTTC AGTCCCGACC 
AGCATGTGGC CCAAAATAAA AGAAGAAACC CAGCATCTCA AGCCTATACA CTTAGAGTAT 
TTTAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCTCAAT CAAAGGATAA TAAAAGAAAT 
TACCAACATA TATGGTTAAT GCCTGCTTCA GAAATGAACT TGTCAGGACT CCCAGAATCA 
ACTTTGTTCA GATTGGACCA GGAAGAAAAA AATTGTGATA TAGATACCCC ATTAGGAAAT 
CTTTCTAAAC TGATGGAAAA TACTCGTGCC AGCCAAAAAC TTCGAGATTT AATTATTGAC 
TTTCGTGAAC CACAGTTTAT ATCCTACCTC AGTTCTGTTC TTCCACATGA GGCAAAGGAC 
ACAGTTGCCT GCATTCTGAA AGGTTTGAAT AAGCCTCAGA GGCAAGCCAT GAAAAAGGTA 
CTTCTTTCAA AAGACTACAC CCTCATCGTG GGCATGCCGG GAACAGGAAA AACAACTACA 
ATATGTACTC TTGTAAGAAT TCTCTATGCC TGTGGTTTTA GTGTTTTGTT GACCAGCTAT 
ACACACTCTG CTGTTGACAA TATTCTTTTG AAGTTAGCAA AGTTTAAAAT TGGATTTTTG 
CGCTTGGGCC AGATTCAGAA GGTACATCCA GATATCCAAA AATTTACAGA GGAGGAAATT 
TGCAGATCAA AGTCCATTAA ATCTTTAGCT CTTCTAGAAG AACTCTACAA CAGTCAACTT 
ATAGTTGCAA CAACATGTAT GGGAATAAAC CATCCCATAT TTTCTCGTAA AACTTTTGAT 
TTTTGTATAG TGGATGAAGC CTCTCAAATT AGCCAGCCTG TTTGCCTAGG GCCACTTTTT 
TTTTCACGGA GATTTGTGTT GGTGGGGGAC CATCAGCAGC TTCCTCCCCT GGTGTTAAAC 
CGTGAAGCAA GAGCTCTTGG CATGAGTGAA AGCTTATTCA AGAGGCTGGA GCAGAATAAG 
AATGCAGTTG TACAGTTAAC TGTGCAATAC AGAATGAACA GTAAAATTAT GTCCTTAAGT 
AATAAACTGA CGTACGAAGG AAAGCTGGAG TGTGGATCAG ATAAAGTGGC CAATGCAGTG 
ATAAACCTGC CTAACTTTAA AGATGTAAAG CTGGAACTGG AGTTTTATGC TGATTATTCA 
GAAAATCCTT GGCTGATTGC AGCATTCGAG CCAAACAATC CTGTCTGTTT TCTTAATACA 
CACAAGGTTC CAGCACCAGA ACAAGTTGAA AAGGGAGGTG TCAGTAATAT AATGGAAGCC 
AAACTCGTTG TTTTCCTGAC GTCAGTTTTT ATTAAGGCTG GTTGTAAACC CTCTGATATT 
GGTATCATTG CACCATACAG GCAGCAGTTA AAGGTCATCA GTGACTTATT GGCACAGTCT 
TCTGTTGGGA TGGTCGAAGT TAATACAGTT GACAGGTACC AAGGAAGAGA CAAAAGTATC 
GTTGTAGTAT CTTTTGTTAG AAGCAATGAA GATGGAACTC TTGGTGAATT GTTGAAAGAT 
TGGCGACGTC TTAATGTCGC TATAACCAGA GCCAAACATA AGCTGATTCT CCTGGGATGT 
GTGCCCTCAC TAAGCCGTTA CCCTCCTTTG AGGAAGCTAC TTAATCATCT CAACTCAGAA 
AAATTAATCA TTGATCTTCC ATCAGGAGAA CATGAAAGCC TTTTTCATCT ATTGGGTGAT 
TTCCAAAGAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNA2 ( XM_024986762.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842530.1 Bos taurus DNA replication helicase/nuclease 2 (DNA2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa54936
Clone ID Related Accession (Same CDS sequence)	XM_024986762.1
Accession Version	XM_024986762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATTG GTACTGTTCT TCATGAAGTA TTTCAAAAAG CAGTAAGTGA TAGTTTTGCC 
CCAGAAAAGC TACAAGAACT TGCTTCTCAA ACTATTCAAG AAATAAGGCA TCTGAAGGAA 
ATGTACCGCC TGAAATTGAA TCAAGATGAA ATAAAACAAG AAGTAGAGGA GTATCTTCCT 
TCATTTTCTA AATGGGCTGG AGATTTCATG CATAAACATA CTTCAACTGA CTTCCCTCAG 
ATGCAGCTCT CTCTGCCAAG TGATGGTAGT AACAGTAATT CAACATGTAA TATTGAAGTT 
ACAAACTCGC TGGATATTGA AGAAAGCATT TGGTCCCCTA GGTTTGGATT GAAGGGCAAA 
ATCGATGTTA CAGTTGGTGT GAAAATACAT CGAGGATGTA AAACGAAATA TAAGATAATG 
CCATTGGAAC TTAAAACTGG CAAAGAATCA AATTCTATTG AACACCGTAG TCAGCTTGTT 
CTGTATACAC TGCTAAGCCA AGAGAGGAGG GCTGACCCAG AGGCCGGCCT GCTCCTCTAC 
CTAAAGACTG GCCAGATGTA CCCTGTGCCT GCCAAACATC TTGATAAAAG AGAATTATTA 
AGGCTGAGAA ATCAGATGGC ATTCTCCTTG TTTCACCGTA TTAACAAATC TACTGGAGAG 
AAGACAGAGC TTGCTCCTTT GCCACAAATA ATTGAGGAAC AGCAAACCTG TAAATACTGT 
TCACAGATGG GCAACTGTGC TCTCTATAGC AGGGCGGTTG AACAACAGAT GGAAGACAGT 
TCAGTCCCGA CCAGCATGTG GCCCAAAATA AAAGAAGAAA CCCAGCATCT CAAGCCTATA 
CACTTAGAGT ATTTTAGCCT TTGGTGTCTA ATGTTAACCC TGGAGTCTCA ATCAAAGGAT 
AATAAAAGAA ATTACCAACA TATATGGTTA ATGCCTGCTT CAGAAATGGA AGAGAGTGGC 
AGCTGCATTG GGAGCCTGAT TAGAATAGAA CATGTAAAAA CAGTTTGTGA TGGACAGTAT 
TTACATAATT TCCAGCGTAA AAATGGAGCC ATACCTATCA CAAATCTAAT GGCAGGTGAC 
AGAATTATTT TAAGTGGAGA AGAGAGAACA TTGTTTGCCT TGTCTAGGGG ATACGTGAAG 
GAGATTAACT CAACAACAGT AACCTGTTCA TTAGACAGGA ACTTGTCAGG ACTCCCAGAA 
TCAACTTTGT TCAGATTGGA CCAGGAAGAA AAAAATTGTG ATATAGATAC CCCATTAGGA 
AATCTTTCTA AACTGATGGA AAATACTCGT GCCAGCCAAA AACTTCGAGA TTTAATTATT 
GACTTTCGTG AACCACAGTT TATATCCTAC CTCAGTTCTG TTCTTCCACA TGAGGCAAAG 
GACACAGTTG CCTGCATTCT GAAAGGTTTG AATAAGCCTC AGAGGCAAGC CATGAAAAAG 
GTACTTCTTT CAAAAGACTA CACCCTCATC GTGGGCATGC CGGGAACAGG AAAAACAACT 
ACAATATGTA CTCTTGTAAG AATTCTCTAT GCCTGTGGTT TTAGTGTTTT GTTGACCAGC 
TATACACACT CTGCTGTTGA CAATATTCTT TTGAAGTTAG CAAAGTTTAA AATTGGATTT 
TTGCGCTTGG GCCAGATTCA GAAGGTACAT CCAGATATCC AAAAATTTAC AGAGGAGGAA 
ATTTGCAGAT CAAAGTCCAT TAAATCTTTA GCTCTTCTAG AAGAACTCTA CAACAGTCAA 
CTTATAGTTG CAACAACATG TATGGGAATA AACCATCCCA TATTTTCTCG TAAAACTTTT 
GATTTTTGTA TAGTGGATGA AGCCTCTCAA ATTAGCCAGC CTGTTTGCCT AGGGCCACTT 
TTTTTTTCAC GGAGATTTGT GTTGGTGGGG GACCATCAGC AGCTTCCTCC CCTGGTGTTA 
AACCGTGAAG CAAGAGCTCT TGGCATGAGT GAAAGCTTAT TCAAGAGGCT GGAGCAGAAT 
AAGAATGCAG TTGTACAGTT AACTGTGCAA TACAGAATGA ACAGTAAAAT TATGTCCTTA 
AGTAATAAAC TGACGTACGA AGGAAAGCTG GAGTGTGGAT CAGATAAAGT GGCCAATGCA 
GTGATAAACC TGCCTAACTT TAAAGATGTA AAGCTGGAAC TGGAGTTTTA TGCTGATTAT 
TCAGAAAATC CTTGGCTGAT TGCAGCATTC GAGCCAAACA ATCCTGTCTG TTTTCTTAAT 
ACACACAAGG TTCCAGCACC AGAACAAGTT GAAAAGGGAG GTGTCAGTAA TATAATGGAA 
GCCAAACTCG TTGTTTTCCT GACGTCAGTT TTTATTAAGG CTGGTTGTAA ACCCTCTGAT 
ATTGGTATCA TTGCACCATA CAGGCAGCAG TTAAAGGTCA TCAGTGACTT ATTGGCACAG 
TCTTCTGTTG GGATGGTCGA AGTTAATACA GTTGACAGGT ACCAAGGAAG AGACAAAAGT 
ATCGTTGTAG TATCTTTTGT TAGAAGCAAT GAAGATGGAA CTCTTGGTGA ATTGTTGAAA 
GATTGGCGAC GTCTTAATGT CGCTATAACC AGAGCCAAAC ATAAGCTGAT TCTCCTGGGA 
TGTGTGCCCT CACTAAGCCG TTACCCTCCT TTGAGGAAGC TACTTAATCA TCTCAACTCA 
GAAAAATTAA TCATTGATCT TCCATCAGGA GAACATGAAA GCCTTTTTCA TCTATTGGGT 
GATTTCCAAA GAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842530.1
CDS	259..2976
Translation	MLIGTVLHEVFQKAVSDSFAPEKLQELASQTIQEIRHLKEMYRLKLNQDEIKQEVEEYLPSFSKWAGDFMHKHTSTDFPQMQLSLPSDGSNSNSTCNIEVTNSLDIEESIWSPRFGLKGKIDVTVGVKIHRGCKTKYKIMPLELKTGKESNSIEHRSQLVLYTLLSQERRADPEAGLLLYLKTGQMYPVPAKHLDKRELLRLRNQMAFSLFHRINKSTGEKTELAPLPQIIEEQQTCKYCSQMGNCALYSRAVEQQMEDSSVPTSMWPKIKEETQHLKPIHLEYFSLWCLMLTLESQSKDNKRNYQHIWLMPASEMEESGSCIGSLIRIEHVKTVCDGQYLHNFQRKNGAIPITNLMAGDRIILSGEERTLFALSRGYVKEINSTTVTCSLDRNLSGLPESTLFRLDQEEKNCDIDTPLGNLSKLMENTRASQKLRDLIIDFREPQFISYLSSVLPHEAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPDIQKFTEEEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKTFDFCIVDEASQISQPVCLGPLFFSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKNAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLPNFKDVKLELEFYADYSENPWLIAAFEPNNPVCFLNTHKVPAPEQVEKGGVSNIMEAKLVVFLTSVFIKAGCKPSDIGIIAPYRQQLKVISDLLAQSSVGMVEVNTVDRYQGRDKSIVVVSFVRSNEDGTLGELLKDWRRLNVAITRAKHKLILLGCVPSLSRYPPLRKLLNHLNSEKLIIDLPSGEHESLFHLLGDFQRK

Target ORF information:

RefSeq Version	XM_024986762.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA replication helicase/nuclease 2 (DNA2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986762.1

ORF Insert Sequence:

ATGCTGATTG GTACTGTTCT TCATGAAGTA TTTCAAAAAG CAGTAAGTGA TAGTTTTGCC 
CCAGAAAAGC TACAAGAACT TGCTTCTCAA ACTATTCAAG AAATAAGGCA TCTGAAGGAA 
ATGTACCGCC TGAAATTGAA TCAAGATGAA ATAAAACAAG AAGTAGAGGA GTATCTTCCT 
TCATTTTCTA AATGGGCTGG AGATTTCATG CATAAACATA CTTCAACTGA CTTCCCTCAG 
ATGCAGCTCT CTCTGCCAAG TGATGGTAGT AACAGTAATT CAACATGTAA TATTGAAGTT 
ACAAACTCGC TGGATATTGA AGAAAGCATT TGGTCCCCTA GGTTTGGATT GAAGGGCAAA 
ATCGATGTTA CAGTTGGTGT GAAAATACAT CGAGGATGTA AAACGAAATA TAAGATAATG 
CCATTGGAAC TTAAAACTGG CAAAGAATCA AATTCTATTG AACACCGTAG TCAGCTTGTT 
CTGTATACAC TGCTAAGCCA AGAGAGGAGG GCTGACCCAG AGGCCGGCCT GCTCCTCTAC 
CTAAAGACTG GCCAGATGTA CCCTGTGCCT GCCAAACATC TTGATAAAAG AGAATTATTA 
AGGCTGAGAA ATCAGATGGC ATTCTCCTTG TTTCACCGTA TTAACAAATC TACTGGAGAG 
AAGACAGAGC TTGCTCCTTT GCCACAAATA ATTGAGGAAC AGCAAACCTG TAAATACTGT 
TCACAGATGG GCAACTGTGC TCTCTATAGC AGGGCGGTTG AACAACAGAT GGAAGACAGT 
TCAGTCCCGA CCAGCATGTG GCCCAAAATA AAAGAAGAAA CCCAGCATCT CAAGCCTATA 
CACTTAGAGT ATTTTAGCCT TTGGTGTCTA ATGTTAACCC TGGAGTCTCA ATCAAAGGAT 
AATAAAAGAA ATTACCAACA TATATGGTTA ATGCCTGCTT CAGAAATGGA AGAGAGTGGC 
AGCTGCATTG GGAGCCTGAT TAGAATAGAA CATGTAAAAA CAGTTTGTGA TGGACAGTAT 
TTACATAATT TCCAGCGTAA AAATGGAGCC ATACCTATCA CAAATCTAAT GGCAGGTGAC 
AGAATTATTT TAAGTGGAGA AGAGAGAACA TTGTTTGCCT TGTCTAGGGG ATACGTGAAG 
GAGATTAACT CAACAACAGT AACCTGTTCA TTAGACAGGA ACTTGTCAGG ACTCCCAGAA 
TCAACTTTGT TCAGATTGGA CCAGGAAGAA AAAAATTGTG ATATAGATAC CCCATTAGGA 
AATCTTTCTA AACTGATGGA AAATACTCGT GCCAGCCAAA AACTTCGAGA TTTAATTATT 
GACTTTCGTG AACCACAGTT TATATCCTAC CTCAGTTCTG TTCTTCCACA TGAGGCAAAG 
GACACAGTTG CCTGCATTCT GAAAGGTTTG AATAAGCCTC AGAGGCAAGC CATGAAAAAG 
GTACTTCTTT CAAAAGACTA CACCCTCATC GTGGGCATGC CGGGAACAGG AAAAACAACT 
ACAATATGTA CTCTTGTAAG AATTCTCTAT GCCTGTGGTT TTAGTGTTTT GTTGACCAGC 
TATACACACT CTGCTGTTGA CAATATTCTT TTGAAGTTAG CAAAGTTTAA AATTGGATTT 
TTGCGCTTGG GCCAGATTCA GAAGGTACAT CCAGATATCC AAAAATTTAC AGAGGAGGAA 
ATTTGCAGAT CAAAGTCCAT TAAATCTTTA GCTCTTCTAG AAGAACTCTA CAACAGTCAA 
CTTATAGTTG CAACAACATG TATGGGAATA AACCATCCCA TATTTTCTCG TAAAACTTTT 
GATTTTTGTA TAGTGGATGA AGCCTCTCAA ATTAGCCAGC CTGTTTGCCT AGGGCCACTT 
TTTTTTTCAC GGAGATTTGT GTTGGTGGGG GACCATCAGC AGCTTCCTCC CCTGGTGTTA 
AACCGTGAAG CAAGAGCTCT TGGCATGAGT GAAAGCTTAT TCAAGAGGCT GGAGCAGAAT 
AAGAATGCAG TTGTACAGTT AACTGTGCAA TACAGAATGA ACAGTAAAAT TATGTCCTTA 
AGTAATAAAC TGACGTACGA AGGAAAGCTG GAGTGTGGAT CAGATAAAGT GGCCAATGCA 
GTGATAAACC TGCCTAACTT TAAAGATGTA AAGCTGGAAC TGGAGTTTTA TGCTGATTAT 
TCAGAAAATC CTTGGCTGAT TGCAGCATTC GAGCCAAACA ATCCTGTCTG TTTTCTTAAT 
ACACACAAGG TTCCAGCACC AGAACAAGTT GAAAAGGGAG GTGTCAGTAA TATAATGGAA 
GCCAAACTCG TTGTTTTCCT GACGTCAGTT TTTATTAAGG CTGGTTGTAA ACCCTCTGAT 
ATTGGTATCA TTGCACCATA CAGGCAGCAG TTAAAGGTCA TCAGTGACTT ATTGGCACAG 
TCTTCTGTTG GGATGGTCGA AGTTAATACA GTTGACAGGT ACCAAGGAAG AGACAAAAGT 
ATCGTTGTAG TATCTTTTGT TAGAAGCAAT GAAGATGGAA CTCTTGGTGA ATTGTTGAAA 
GATTGGCGAC GTCTTAATGT CGCTATAACC AGAGCCAAAC ATAAGCTGAT TCTCCTGGGA 
TGTGTGCCCT CACTAAGCCG TTACCCTCCT TTGAGGAAGC TACTTAATCA TCTCAACTCA 
GAAAAATTAA TCATTGATCT TCCATCAGGA GAACATGAAA GCCTTTTTCA TCTATTGGGT 
GATTTCCAAA GAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNA2 ( NM_001192802.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179731.2 Bos taurus DNA replication helicase/nuclease 2 (DNA2), mRNA.

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CloneID	OBa44431
Clone ID Related Accession (Same CDS sequence)	NM_001192802.2
Accession Version	NM_001192802.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(cattle)
Product	DNA replication ATP-dependent helicase/nuclease DNA2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03026586.1. On Apr 16, 2011 this sequence version replaced NM_001192802.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCGAG TGGACGAACT GGAGCTGCTA ATGGAGAAGA GTTTCTGGCA GGAGGCTGAG 
CCGTCAGCGG AGCTATTTCA GAAGAAGAAG GTGGAAGCTT CCTTTTCTAA GATAGTTCTG 
AGCAGAGGAA TGGATAACCG GTACCTGGTA TTAGCAGTCG ATATTGTACA GAGTGAAGAA 
GGAAACCATG AAAAGCACCT AATCATCACT GCTTCCCAGT CGCTAGAATA TAAAGAACTG 
TGTATCCTTA GGAATGACTG GTGCTCTGTA CCAGTAGAGC CAGGGGACAT CATTCATTTG 
GAAGGAGACT GCATATCCAA TACTTGGATA ATAGATGAAG ATTTTGGATA TTTGATTCTA 
TATCCCGACA TGCTGATTTC TGGCACAAGC ATAGCCAGTA GTATTCGATG CATGAGAAGA 
GCTGTTCTGA GTGAAACATT CAGGAGCTCT GATCCAGCCA CACGCCAAAT GCTGATTGGT 
ACTGTTCTTC ATGAAGTATT TCAAAAAGCA GTAAGTGATA GTTTTGCCCC AGAAAAGCTA 
CAAGAACTTG CTTCTCAAAC TATTCAAGAA ATAAGGCATC TGAAGGAAAT GTACCGCCTG 
AAATTGAATC AAGATGAAAT AAAACAAGAA GTAGAGGAGT ATCTTCCTTC ATTTTCTAAA 
TGGGCTGGAG ATTTCATGCA TAAACATACT TCAACTGACT TCCCTCAGAT GCAGCTCTCT 
CTGCCAAGTG ATGGTAGTAA CAGTAATTCA ACATGTAATA TTGAAGTTAC AAACTCGCTG 
GATATTGAAG AAAGCATTTG GTCCCCTAGG TTTGGATTGA AGGGCAAAAT CGATGTTACA 
GTTGGTGTGA AAATACATCG AGGATGTAAA ACGAAATATA AGATAATGCC ATTGGAACTT 
AAAACTGGCA AAGAATCAAA TTCTATTGAA CACCGTAGTC AGCTTGTTCT GTATACACTG 
CTAAGCCAAG AGAGGAGGGC TGACCCAGAG GCCGGCCTGC TCCTCTACCT AAAGACTGGC 
CAGATGTACC CTGTGCCTGC CAAACATCTT GATAAAAGAG AATTATTAAG GCTGAGAAAT 
CAGATGGCAT TCTCCTTGTT TCACCGTATT AACAAATCTA CTGGAGAGAA GACAGAGCTT 
GCTCCTTTGC CACAAATAAT TGAGGAACAG CAAACCTGTA AATACTGTTC ACAGATGGGC 
AACTGTGCTC TCTATAGCAG GGCGGTTGAA CAACAGATGG AAGACAGTTC AGTCCCGACC 
AGCATGTGGC CCAAAATAAA AGAAGAAACC CAGCATCTCA AGCCTATACA CTTAGAGTAT 
TTTAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCTCAAT CAAAGGATAA TAAAAGAAAT 
TACCAACATA TATGGTTAAT GCCTGCTTCA GAAATGGAAG AGAGTGGCAG CTGCATTGGG 
AGCCTGATTA GAATAGAACA TGTAAAAACA GTTTGTGATG GACAGTATTT ACATAATTTC 
CAGCGTAAAA ATGGAGCCAT ACCTATCACA AATCTAATGG CAGGTGACAG AATTATTTTA 
AGTGGAGAAG AGAGAACATT GTTTGCCTTG TCTAGGGGAT ACGTGAAGGA GATTAACTCA 
ACAACAGTAA CCTGTTCATT AGACAGGAAC TTGTCAGGAC TCCCAGAATC AACTTTGTTC 
AGATTGGACC AGGAAGAAAA AAATTGTGAT ATAGATACCC CATTAGGAAA TCTTTCTAAA 
CTGATGGAAA ATACTCGTGC CAGCCAAAAA CTTCGAGATT TAATTATTGA CTTTCGTGAA 
CCACAGTTTA TATCCTACCT CAGTTCTGTT CTTCCACATG AGGCAAAGGA CACAGTTGCC 
TGCATTCTGA AAGGTTTGAA TAAGCCTCAG AGGCAAGCCA TGAAAAAGGT ACTTCTTTCA 
AAAGACTACA CCCTCATCGT GGGCATGCCG GGAACAGGAA AAACAACTAC AATATGTACT 
CTTGTAAGAA TTCTCTATGC CTGTGGTTTT AGTGTTTTGT TGACCAGCTA TACACACTCT 
GCTGTTGACA ATATTCTTTT GAAGTTAGCA AAGTTTAAAA TTGGATTTTT GCGCTTGGGC 
CAGATTCAGA AGGTACATCC AGATATCCAA AAATTTACAG AGGAGGAAAT TTGCAGATCA 
AAGTCCATTA AATCTTTAGC TCTTCTAGAA GAACTCTACA ACAGTCAACT TATAGTTGCA 
ACAACATGTA TGGGAATAAA CCATCCCATA TTTTCTCGTA AAACTTTTGA TTTTTGTATA 
GTGGATGAAG CCTCTCAAAT TAGCCAGCCT GTTTGCCTAG GGCCACTTTT TTTTTCACGG 
AGATTTGTGT TGGTGGGGGA CCATCAGCAG CTTCCTCCCC TGGTGTTAAA CCGTGAAGCA 
AGAGCTCTTG GCATGAGTGA AAGCTTATTC AAGAGGCTGG AGCAGAATAA GAATGCAGTT 
GTACAGTTAA CTGTGCAATA CAGAATGAAC AGTAAAATTA TGTCCTTAAG TAATAAACTG 
ACGTACGAAG GAAAGCTGGA GTGTGGATCA GATAAAGTGG CCAATGCAGT GATAAACCTG 
CCTAACTTTA AAGATGTAAA GCTGGAACTG GAGTTTTATG CTGATTATTC AGAAAATCCT 
TGGCTGATTG CAGCATTCGA GCCAAACAAT CCTGTCTGTT TTCTTAATAC ACACAAGGTT 
CCAGCACCAG AACAAGTTGA AAAGGGAGGT GTCAGTAATA TAATGGAAGC CAAACTCGTT 
GTTTTCCTGA CGTCAGTTTT TATTAAGGCT GGTTGTAAAC CCTCTGATAT TGGTATCATT 
GCACCATACA GGCAGCAGTT AAAGGTCATC AGTGACTTAT TGGCACAGTC TTCTGTTGGG 
ATGGTCGAAG TTAATACAGT TGACAGGTAC CAAGGAAGAG ACAAAAGTAT CGTTGTAGTA 
TCTTTTGTTA GAAGCAATGA AGATGGAACT CTTGGTGAAT TGTTGAAAGA TTGGCGACGT 
CTTAATGTCG CTATAACCAG AGCCAAACAT AAGCTGATTC TCCTGGGATG TGTGCCCTCA 
CTAAGCCGTT ACCCTCCTTT GGGGAAGCTA CTTAATCATC TCAACTCAGA AAAATTAATC 
ATTGATCTTC CATCAGGAGA ACATGAAAGC CTTTTTCATC TATTGGGTGA TTTCCAAAGA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179731.2
CDS	62..3247
Translation	MERVDELELLMEKSFWQEAEPSAELFQKKKVEASFSKIVLSRGMDNRYLVLAVDIVQSEEGNHEKHLIITASQSLEYKELCILRNDWCSVPVEPGDIIHLEGDCISNTWIIDEDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRSSDPATRQMLIGTVLHEVFQKAVSDSFAPEKLQELASQTIQEIRHLKEMYRLKLNQDEIKQEVEEYLPSFSKWAGDFMHKHTSTDFPQMQLSLPSDGSNSNSTCNIEVTNSLDIEESIWSPRFGLKGKIDVTVGVKIHRGCKTKYKIMPLELKTGKESNSIEHRSQLVLYTLLSQERRADPEAGLLLYLKTGQMYPVPAKHLDKRELLRLRNQMAFSLFHRINKSTGEKTELAPLPQIIEEQQTCKYCSQMGNCALYSRAVEQQMEDSSVPTSMWPKIKEETQHLKPIHLEYFSLWCLMLTLESQSKDNKRNYQHIWLMPASEMEESGSCIGSLIRIEHVKTVCDGQYLHNFQRKNGAIPITNLMAGDRIILSGEERTLFALSRGYVKEINSTTVTCSLDRNLSGLPESTLFRLDQEEKNCDIDTPLGNLSKLMENTRASQKLRDLIIDFREPQFISYLSSVLPHEAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPDIQKFTEEEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKTFDFCIVDEASQISQPVCLGPLFFSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKNAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLPNFKDVKLELEFYADYSENPWLIAAFEPNNPVCFLNTHKVPAPEQVEKGGVSNIMEAKLVVFLTSVFIKAGCKPSDIGIIAPYRQQLKVISDLLAQSSVGMVEVNTVDRYQGRDKSIVVVSFVRSNEDGTLGELLKDWRRLNVAITRAKHKLILLGCVPSLSRYPPLGKLLNHLNSEKLIIDLPSGEHESLFHLLGDFQRK

Target ORF information:

RefSeq Version	NM_001192802.2
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA replication helicase/nuclease 2 (DNA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192802.2

ORF Insert Sequence:

ATGGAGCGAG TGGACGAACT GGAGCTGCTA ATGGAGAAGA GTTTCTGGCA GGAGGCTGAG 
CCGTCAGCGG AGCTATTTCA GAAGAAGAAG GTGGAAGCTT CCTTTTCTAA GATAGTTCTG 
AGCAGAGGAA TGGATAACCG GTACCTGGTA TTAGCAGTCG ATATTGTACA GAGTGAAGAA 
GGAAACCATG AAAAGCACCT AATCATCACT GCTTCCCAGT CGCTAGAATA TAAAGAACTG 
TGTATCCTTA GGAATGACTG GTGCTCTGTA CCAGTAGAGC CAGGGGACAT CATTCATTTG 
GAAGGAGACT GCATATCCAA TACTTGGATA ATAGATGAAG ATTTTGGATA TTTGATTCTA 
TATCCCGACA TGCTGATTTC TGGCACAAGC ATAGCCAGTA GTATTCGATG CATGAGAAGA 
GCTGTTCTGA GTGAAACATT CAGGAGCTCT GATCCAGCCA CACGCCAAAT GCTGATTGGT 
ACTGTTCTTC ATGAAGTATT TCAAAAAGCA GTAAGTGATA GTTTTGCCCC AGAAAAGCTA 
CAAGAACTTG CTTCTCAAAC TATTCAAGAA ATAAGGCATC TGAAGGAAAT GTACCGCCTG 
AAATTGAATC AAGATGAAAT AAAACAAGAA GTAGAGGAGT ATCTTCCTTC ATTTTCTAAA 
TGGGCTGGAG ATTTCATGCA TAAACATACT TCAACTGACT TCCCTCAGAT GCAGCTCTCT 
CTGCCAAGTG ATGGTAGTAA CAGTAATTCA ACATGTAATA TTGAAGTTAC AAACTCGCTG 
GATATTGAAG AAAGCATTTG GTCCCCTAGG TTTGGATTGA AGGGCAAAAT CGATGTTACA 
GTTGGTGTGA AAATACATCG AGGATGTAAA ACGAAATATA AGATAATGCC ATTGGAACTT 
AAAACTGGCA AAGAATCAAA TTCTATTGAA CACCGTAGTC AGCTTGTTCT GTATACACTG 
CTAAGCCAAG AGAGGAGGGC TGACCCAGAG GCCGGCCTGC TCCTCTACCT AAAGACTGGC 
CAGATGTACC CTGTGCCTGC CAAACATCTT GATAAAAGAG AATTATTAAG GCTGAGAAAT 
CAGATGGCAT TCTCCTTGTT TCACCGTATT AACAAATCTA CTGGAGAGAA GACAGAGCTT 
GCTCCTTTGC CACAAATAAT TGAGGAACAG CAAACCTGTA AATACTGTTC ACAGATGGGC 
AACTGTGCTC TCTATAGCAG GGCGGTTGAA CAACAGATGG AAGACAGTTC AGTCCCGACC 
AGCATGTGGC CCAAAATAAA AGAAGAAACC CAGCATCTCA AGCCTATACA CTTAGAGTAT 
TTTAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCTCAAT CAAAGGATAA TAAAAGAAAT 
TACCAACATA TATGGTTAAT GCCTGCTTCA GAAATGGAAG AGAGTGGCAG CTGCATTGGG 
AGCCTGATTA GAATAGAACA TGTAAAAACA GTTTGTGATG GACAGTATTT ACATAATTTC 
CAGCGTAAAA ATGGAGCCAT ACCTATCACA AATCTAATGG CAGGTGACAG AATTATTTTA 
AGTGGAGAAG AGAGAACATT GTTTGCCTTG TCTAGGGGAT ACGTGAAGGA GATTAACTCA 
ACAACAGTAA CCTGTTCATT AGACAGGAAC TTGTCAGGAC TCCCAGAATC AACTTTGTTC 
AGATTGGACC AGGAAGAAAA AAATTGTGAT ATAGATACCC CATTAGGAAA TCTTTCTAAA 
CTGATGGAAA ATACTCGTGC CAGCCAAAAA CTTCGAGATT TAATTATTGA CTTTCGTGAA 
CCACAGTTTA TATCCTACCT CAGTTCTGTT CTTCCACATG AGGCAAAGGA CACAGTTGCC 
TGCATTCTGA AAGGTTTGAA TAAGCCTCAG AGGCAAGCCA TGAAAAAGGT ACTTCTTTCA 
AAAGACTACA CCCTCATCGT GGGCATGCCG GGAACAGGAA AAACAACTAC AATATGTACT 
CTTGTAAGAA TTCTCTATGC CTGTGGTTTT AGTGTTTTGT TGACCAGCTA TACACACTCT 
GCTGTTGACA ATATTCTTTT GAAGTTAGCA AAGTTTAAAA TTGGATTTTT GCGCTTGGGC 
CAGATTCAGA AGGTACATCC AGATATCCAA AAATTTACAG AGGAGGAAAT TTGCAGATCA 
AAGTCCATTA AATCTTTAGC TCTTCTAGAA GAACTCTACA ACAGTCAACT TATAGTTGCA 
ACAACATGTA TGGGAATAAA CCATCCCATA TTTTCTCGTA AAACTTTTGA TTTTTGTATA 
GTGGATGAAG CCTCTCAAAT TAGCCAGCCT GTTTGCCTAG GGCCACTTTT TTTTTCACGG 
AGATTTGTGT TGGTGGGGGA CCATCAGCAG CTTCCTCCCC TGGTGTTAAA CCGTGAAGCA 
AGAGCTCTTG GCATGAGTGA AAGCTTATTC AAGAGGCTGG AGCAGAATAA GAATGCAGTT 
GTACAGTTAA CTGTGCAATA CAGAATGAAC AGTAAAATTA TGTCCTTAAG TAATAAACTG 
ACGTACGAAG GAAAGCTGGA GTGTGGATCA GATAAAGTGG CCAATGCAGT GATAAACCTG 
CCTAACTTTA AAGATGTAAA GCTGGAACTG GAGTTTTATG CTGATTATTC AGAAAATCCT 
TGGCTGATTG CAGCATTCGA GCCAAACAAT CCTGTCTGTT TTCTTAATAC ACACAAGGTT 
CCAGCACCAG AACAAGTTGA AAAGGGAGGT GTCAGTAATA TAATGGAAGC CAAACTCGTT 
GTTTTCCTGA CGTCAGTTTT TATTAAGGCT GGTTGTAAAC CCTCTGATAT TGGTATCATT 
GCACCATACA GGCAGCAGTT AAAGGTCATC AGTGACTTAT TGGCACAGTC TTCTGTTGGG 
ATGGTCGAAG TTAATACAGT TGACAGGTAC CAAGGAAGAG ACAAAAGTAT CGTTGTAGTA 
TCTTTTGTTA GAAGCAATGA AGATGGAACT CTTGGTGAAT TGTTGAAAGA TTGGCGACGT 
CTTAATGTCG CTATAACCAG AGCCAAACAT AAGCTGATTC TCCTGGGATG TGTGCCCTCA 
CTAAGCCGTT ACCCTCCTTT GGGGAAGCTA CTTAATCATC TCAACTCAGA AAAATTAATC 
ATTGATCTTC CATCAGGAGA ACATGAAAGC CTTTTTCATC TATTGGGTGA TTTCCAAAGA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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DNA2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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