DNA2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNA2
Entrez Gene ID	1763
Full Name	DNA replication helicase/nuclease 2
Synonyms	DNA2L,hDNA2
General protein information	Preferred Names DNA replication helicase/nuclease 2 Names DNA replication ATP-dependent helicase/nuclease DNA2 DNA replication ATP-dependent helicase-like homolog DNA replication helicase 2 homolog DNA2 DNA replication helicase 2-like DNA2-like helicase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q21.3
Summary	This gene encodes a member of the DNA2/NAM7 helicase family. The encoded protein is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability. Mutations in this gene are associated with autosomal dominant progressive external ophthalmoplegia-6 (PEOA6) and Seckel syndrome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014].
Disorder MIM:	601810
Disorder Html:	Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant 6, 615156 (3); ?Seckel syndrome 8, 615807 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001080449.2	NP_001073918.2	DNA replication ATP-dependent helicase/nuclease DNA2
XM_006717680.3	XP_006717743.1	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X1
XM_011539417.1	XP_011537719.1	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X3
NM_001080449.3	NP_001073918.2	DNA replication ATP-dependent helicase/nuclease DNA2
XM_017015799.1	XP_016871288.1	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DNA2	NP_001006497.1
Bos taurus (cattle)	DNA2	NP_001179731.2
Rattus norvegicus (Norway rat)	Dna2	XP_241671.6
Arabidopsis thaliana (thale cress)	emb2411	NP_001184943.1
Oryza sativa (rice)	Os04g0588200	NP_001053694.2
Canis lupus familiaris (dog)	DNA2	XP_005619489.1
Danio rerio (zebrafish)	dna2	XP_687974.5
Pan troglodytes (chimpanzee)	DNA2	XP_001163213.2
Mus musculus (house mouse)	Dna2	NP_796346.2
Drosophila melanogaster (fruit fly)	CG2990	NP_727386.1
Homo sapiens (human)	DNA2	NP_001073918.2
	DNA2	XP_002808547.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004685	XP_307265.4
Caenorhabditis elegans (roundworm)	dna-2	NP_496515.1
Schizosaccharomyces pombe (fission yeast)	dna2	NP_596499.2
Magnaporthe oryzae (rice blast fungus)	MGG_02491	XP_003709288.1
Xenopus tropicalis (tropical clawed frog)	dna2	XP_002936016.2

Gene Ontology
Bibliography

Related articles in PubMed

Role of human DNA2 (hDNA2) as a potential target for cancer and other diseases: A systematic review.
Jia PP, Junaid M, Ma YB, Ahmad F, Jia YF, Li WG, Pei DS
DNA repair599-19(2017 Nov)

The motor activity of DNA2 functions as an ssDNA translocase to promote DNA end resection.
Levikova M, Pinto C, Cejka P
Genes & development31(5)493-502(2017 Mar)

A balance between elongation and trimming regulates telomere stability in stem cells.
Rivera T, Haggblom C, Cosconati S, Karlseder J
Nature structural & molecular biology24(1)30-39(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This review mainly encompasses the key functions of DNA2 in human cells with various aspects, especially focusing on the genome integrity, and also generalizes the recent insights to the mechanisms related to the occurrence of cancer and other diseases potentially linked to the mutations in DNA2.
Title: Role of human DNA2 (hDNA2) as a potential target for cancer and other diseases: A systematic review.

the motor of DNA2 functions as a ssDNA translocase to promote degradation of 5'-terminated DNA.
Title: The motor activity of DNA2 functions as an ssDNA translocase to promote DNA end resection.

The authors show that the helicase of hDNA2 functionally integrates with BLM or WRN helicases to promote double-stranded DNA degradation by forming a heterodimeric molecular machine. This collectively suggests that the human DNA2 motor promotes the enzyme's capacity to degrade double-stranded DNA in conjunction with BLM or WRN and thus promote the repair of broken DNA.
Title: Human DNA2 possesses a cryptic DNA unwinding activity that functionally integrates with BLM or WRN helicases.

EXO1, MRE11, and CtIP are not involved in the same mechanism of reversed fork processing, whereas human RECQ1 limits DNA2 activity by preventing extensive nascent strand degradation.
Title: DNA2 drives processing and restart of reversed replication forks in human cells.

BRCA1 and CtIP contribute to DSB resection by recruiting Dna2 to damage sites, thus ensuring the robust DSB resection necessary for efficient homologous recombination.
Title: BRCA1 and CtIP Are Both Required to Recruit Dna2 at Double-Strand Breaks in Homologous Recombination.

The following DNA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu57308	XM_011539417.1 Latest version!	Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu91165	XM_017015799.1 Latest version!	Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu27848	NM_001080449.3 Latest version!	Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$811.30 $1159.00
OHu44095	XM_006717680.3 Latest version!	Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu27848	NM_001080449.2	Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNA2 ( XM_011539417.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537719.1 Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu57308
Clone ID Related Accession (Same CDS sequence)	XM_011539417.1
Accession Version	XM_011539417.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCT CATTGTTTCA CCGTATTAGC AAATCTGCTA CTAGACAGAA GACACAGCTT 
GCTTCTTTGC CACAAATAAT TGAGGAAGAG AAAACTTGTA AATATTGTTC ACAAATTGGC 
AATTGTGCTC TTTATAGCAG AGCAGTTGAA CAACAGATGG ATTGTAGTTC AGTCCCAATT 
GTGATGCTGC CCAAAATAGA AGAAGAAACC CAGCATCTGA AGCAAACACA CTTAGAATAT 
TTCAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCACAAT CGAAGGATAA TAAAAAGAAT 
CACCAAAATA TCTGGCTAAT GCCTGCTTCG GAAATGGAGA AGAGTGGCAG TTGCATTGGA 
AACCTGATTA GAATGGAACA TGTAAAGATA GTTTGTGATG GGCAATATTT ACATAATTTC 
CAATGTAAAC ATGGTGCCAT ACCTGTCACA AATCTAATGG CAGGTGACAG AGTTATTGTA 
AGTGGAGAAG AAAGGTCACT GTTTGCTTTG TCTAGAGGAT ATGTGAAGGA GATTAACATG 
ACAACAGTAA CTTGTTTATT AGACAGAAAC TTGTCGGTCC TTCCAGAATC AACTTTGTTC 
AGATTAGACC AAGAAGAAAA AAATTGTGAT ATAGATACCC CATTAGGAAA TCTTTCCAAA 
TTGATGGAAA ACACGTTTGT CAGCAAAAAA CTTCGAGATT TAATTATTGA CTTTCGTGAA 
CCTCAGTTTA TATCCTACCT TAGTTCTGTT CTTCCACATG ATGCAAAGGA TACAGTTGCC 
TGCATTCTAA AGGGTTTGAA TAAGCCTCAG AGGCAAGCGA TGAAAAAGGT ACTTCTTTCA 
AAAGACTACA CACTCATCGT GGGTATGCCT GGGACAGGAA AAACAACTAC GATATGTACT 
CTCGTAAGAA TTCTCTACGC CTGTGGTTTT AGCGTTTTGT TGACCAGCTA TACACACTCT 
GCTGTTGACA ATATTCTTTT GAAGTTAGCC AAGTTTAAAA TAGGATTTTT GCGTTTGGGT 
CAGATTCAGA AGGTTCATCC AGCTATCCAG CAATTTACAG AGCAAGAAAT TTGCAGATCA 
AAGTCCATTA AATCCTTAGC TCTTCTAGAA GAACTCTACA ATAGTCAACT TATAGTTGCA 
ACAACATGTA TGGGAATAAA CCATCCAATA TTTTCCCGTA AAATTTTTGA TTTTTGTATT 
GTGGATGAAG CCTCTCAAAT TAGCCAACCA ATTTGTCTGG GCCCCCTTTT TTTTTCACGG 
AGATTTGTGT TAGTGGGGGA CCATCAGCAG CTTCCTCCCC TGGTGCTAAA CCGTGAAGCA 
AGAGCTCTTG GCATGAGTGA AAGCTTATTC AAGAGGCTGG AGCAGAATAA GAGTGCTGTT 
GTACAGTTAA CCGTGCAGTA CAGAATGAAC AGTAAAATTA TGTCCTTAAG TAATAAGCTG 
ACCTATGAGG GCAAGCTGGA GTGTGGATCA GACAAAGTGG CCAATGCAGT GATAAACCTA 
CGTCACTTTA AAGATGTGAA GCTGGAACTG GAATTTTATG CTGACTATTC TGATAATCCT 
TGGTTGATGG GAGTATTTGA ACCCAACAAT CCTGTTTGTT TCCTTAATAC AGACAAGGTT 
CCAGCGCCAG AACAAGTTGA AAAAGGTGGT GTGAGCAATG TAACAGAAGC CAAACTCATA 
GTTTTCCTAA CCTCCATTTT TGTTAAGGCT GGATGCAGTC CCTCTGATAT TGGTATTATT 
GCACCGTACA GGCAGCAATT AAAGATCATC AATGATTTAT TGGCACGTTC TATTGGGATG 
GTCGAAGTTA ATACAGTAGA CAAATACCAA GGAAGGGACA AAAGTATTGT CCTAGTATCT 
TTTGTTAGAA GTAATAAGGA TGGAACTGTT GGTGAACTCT TGAAAGATTG GCGACGTCTT 
AATGTTGCTA TAACCAGAGC CAAACATAAA CTGATTCTTC TGGGGTGTGT GCCCTCACTA 
AATTGCTATC CTCCTTTGGA GAAGCTGCTT AATCATTTAA ACTCAGAAAA ATTAATCATT 
GATCTTCCAT CAAGAGAACA TGAAAGTCTT TGCCACATAT TGGGTGACTT TCAAAGAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537719.1
CDS	114..2216
Translation	MAFSLFHRISKSATRQKTQLASLPQIIEEEKTCKYCSQIGNCALYSRAVEQQMDCSSVPIVMLPKIEEETQHLKQTHLEYFSLWCLMLTLESQSKDNKKNHQNIWLMPASEMEKSGSCIGNLIRMEHVKIVCDGQYLHNFQCKHGAIPVTNLMAGDRVIVSGEERSLFALSRGYVKEINMTTVTCLLDRNLSVLPESTLFRLDQEEKNCDIDTPLGNLSKLMENTFVSKKLRDLIIDFREPQFISYLSSVLPHDAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPAIQQFTEQEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKIFDFCIVDEASQISQPICLGPLFFSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKSAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLRHFKDVKLELEFYADYSDNPWLMGVFEPNNPVCFLNTDKVPAPEQVEKGGVSNVTEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLARSIGMVEVNTVDKYQGRDKSIVLVSFVRSNKDGTVGELLKDWRRLNVAITRAKHKLILLGCVPSLNCYPPLEKLLNHLNSEKLIIDLPSREHESLCHILGDFQRE

Target ORF information:

RefSeq Version	XM_011539417.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539417.1

ORF Insert Sequence:

ATGGCATTCT CATTGTTTCA CCGTATTAGC AAATCTGCTA CTAGACAGAA GACACAGCTT 
GCTTCTTTGC CACAAATAAT TGAGGAAGAG AAAACTTGTA AATATTGTTC ACAAATTGGC 
AATTGTGCTC TTTATAGCAG AGCAGTTGAA CAACAGATGG ATTGTAGTTC AGTCCCAATT 
GTGATGCTGC CCAAAATAGA AGAAGAAACC CAGCATCTGA AGCAAACACA CTTAGAATAT 
TTCAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCACAAT CGAAGGATAA TAAAAAGAAT 
CACCAAAATA TCTGGCTAAT GCCTGCTTCG GAAATGGAGA AGAGTGGCAG TTGCATTGGA 
AACCTGATTA GAATGGAACA TGTAAAGATA GTTTGTGATG GGCAATATTT ACATAATTTC 
CAATGTAAAC ATGGTGCCAT ACCTGTCACA AATCTAATGG CAGGTGACAG AGTTATTGTA 
AGTGGAGAAG AAAGGTCACT GTTTGCTTTG TCTAGAGGAT ATGTGAAGGA GATTAACATG 
ACAACAGTAA CTTGTTTATT AGACAGAAAC TTGTCGGTCC TTCCAGAATC AACTTTGTTC 
AGATTAGACC AAGAAGAAAA AAATTGTGAT ATAGATACCC CATTAGGAAA TCTTTCCAAA 
TTGATGGAAA ACACGTTTGT CAGCAAAAAA CTTCGAGATT TAATTATTGA CTTTCGTGAA 
CCTCAGTTTA TATCCTACCT TAGTTCTGTT CTTCCACATG ATGCAAAGGA TACAGTTGCC 
TGCATTCTAA AGGGTTTGAA TAAGCCTCAG AGGCAAGCGA TGAAAAAGGT ACTTCTTTCA 
AAAGACTACA CACTCATCGT GGGTATGCCT GGGACAGGAA AAACAACTAC GATATGTACT 
CTCGTAAGAA TTCTCTACGC CTGTGGTTTT AGCGTTTTGT TGACCAGCTA TACACACTCT 
GCTGTTGACA ATATTCTTTT GAAGTTAGCC AAGTTTAAAA TAGGATTTTT GCGTTTGGGT 
CAGATTCAGA AGGTTCATCC AGCTATCCAG CAATTTACAG AGCAAGAAAT TTGCAGATCA 
AAGTCCATTA AATCCTTAGC TCTTCTAGAA GAACTCTACA ATAGTCAACT TATAGTTGCA 
ACAACATGTA TGGGAATAAA CCATCCAATA TTTTCCCGTA AAATTTTTGA TTTTTGTATT 
GTGGATGAAG CCTCTCAAAT TAGCCAACCA ATTTGTCTGG GCCCCCTTTT TTTTTCACGG 
AGATTTGTGT TAGTGGGGGA CCATCAGCAG CTTCCTCCCC TGGTGCTAAA CCGTGAAGCA 
AGAGCTCTTG GCATGAGTGA AAGCTTATTC AAGAGGCTGG AGCAGAATAA GAGTGCTGTT 
GTACAGTTAA CCGTGCAGTA CAGAATGAAC AGTAAAATTA TGTCCTTAAG TAATAAGCTG 
ACCTATGAGG GCAAGCTGGA GTGTGGATCA GACAAAGTGG CCAATGCAGT GATAAACCTA 
CGTCACTTTA AAGATGTGAA GCTGGAACTG GAATTTTATG CTGACTATTC TGATAATCCT 
TGGTTGATGG GAGTATTTGA ACCCAACAAT CCTGTTTGTT TCCTTAATAC AGACAAGGTT 
CCAGCGCCAG AACAAGTTGA AAAAGGTGGT GTGAGCAATG TAACAGAAGC CAAACTCATA 
GTTTTCCTAA CCTCCATTTT TGTTAAGGCT GGATGCAGTC CCTCTGATAT TGGTATTATT 
GCACCGTACA GGCAGCAATT AAAGATCATC AATGATTTAT TGGCACGTTC TATTGGGATG 
GTCGAAGTTA ATACAGTAGA CAAATACCAA GGAAGGGACA AAAGTATTGT CCTAGTATCT 
TTTGTTAGAA GTAATAAGGA TGGAACTGTT GGTGAACTCT TGAAAGATTG GCGACGTCTT 
AATGTTGCTA TAACCAGAGC CAAACATAAA CTGATTCTTC TGGGGTGTGT GCCCTCACTA 
AATTGCTATC CTCCTTTGGA GAAGCTGCTT AATCATTTAA ACTCAGAAAA ATTAATCATT 
GATCTTCCAT CAAGAGAACA TGAAAGTCTT TGCCACATAT TGGGTGACTT TCAAAGAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNA2 ( XM_017015799.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871288.1 Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu91165
Clone ID Related Accession (Same CDS sequence)	XM_017015799.1
Accession Version	XM_017015799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAAAA ACACTTCGAC TGACTTCCCT CAGATGCAGC TCTCTCTGCC AAGTGATAAT 
AGTAAGGATA ATTCAACATG TAACATTGAA GTCGTGAAAC CAATGGATAT TGAAGAAAGC 
ATTTGGTCCC CTAGGTTTGG ATTGAAAGGC AAAATAGATG TTACAGTTGG TGTGAAAATA 
CATCGAGGGT ATAAAACAAA ATACAAGATA ATGCCGCTGG AACTTAAAAC TGGCAAAGAA 
TCAAATTCTA TTGAACACCG TAGTCAGGTT GTTCTGTACA CTCTACTAAG CCAAGAGAGA 
AGAGCTGATC CAGAGGCTGG CTTGCTTCTC TACCTCAAGA CTGGTCAGAT GTACCCTGTG 
CCTGCCAACC ATCTAGATAA AAGAGAATTA TTAAAGCTAA GAAACCAGAT GGCATTCTCA 
TTGTTTCACC GTATTAGCAA ATCTGCTACT AGACAGAAGA CACAGCTTGC TTCTTTGCCA 
CAAATAATTG AGGAAGAGAA AACTTGTAAA TATTGTTCAC AAATTGGCAA TTGTGCTCTT 
TATAGCAGAG CAGTTGAACA ACAGATGGAT TGTAGTTCAG TCCCAATTGT GATGCTGCCC 
AAAATAGAAG AAGAAACCCA GCATCTGAAG CAAACACACT TAGAATATTT CAGCCTTTGG 
TGTCTAATGT TAACCCTGGA GTCACAATCG AAGGATAATA AAAAGAATCA CCAAAATATC 
TGGCTAATGC CTGCTTCGGA AATGGAGAAG AGTGGCAGTT GCATTGGAAA CCTGATTAGA 
ATGGAACATG TAAAGATAGT TTGTGATGGG CAATATTTAC ATAATTTCCA ATGTAAACAT 
GGTGCCATAC CTGTCACAAA TCTAATGGCA GGTGACAGAG TTATTGTAAG TGGAGAAGAA 
AGGTCACTGT TTGCTTTGTC TAGAGGATAT GTGAAGGAGA TTAACATGAC AACAGTAACT 
TGTTTATTAG ACAGAAACTT GTCGGTCCTT CCAGAATCAA CTTTGTTCAG ATTAGACCAA 
GAAGAAAAAA ATTGTGATAT AGATACCCCA TTAGGAAATC TTTCCAAATT GATGGAAAAC 
ACGTTTGTCA GCAAAAAACT TCGAGATTTA ATTATTGACT TTCGTGAACC TCAGTTTATA 
TCCTACCTTA GTTCTGTTCT TCCACATGAT GCAAAGGATA CAGTTGCCTG CATTCTAAAG 
GGTTTGAATA AGCCTCAGAG GCAAGCGATG AAAAAGGTAC TTCTTTCAAA AGACTACACA 
CTCATCGTGG GTATGCCTGG GACAGGAAAA ACAACTACGA TATGTACTCT CGTAAGAATT 
CTCTACGCCT GTGGTTTTAG CGTTTTGTTG ACCAGCTATA CACACTCTGC TGTTGACAAT 
ATTCTTTTGA AGTTAGCCAA GTTTAAAATA GGATTTTTGC GTTTGGGTCA GATTCAGAAG 
GTTCATCCAG CTATCCAGCA ATTTACAGAG CAAGAAATTT GCAGATCAAA GTCCATTAAA 
TCCTTAGCTC TTCTAGAAGA ACTCTACAAT AGTCAACTTA TAGTTGCAAC AACATGTATG 
GGAATAAACC ATCCAATATT TTCCCGTAAA ATTTTTGATT TTTGTATTGT GGATGAAGCC 
TCTCAAATTA GCCAACCAAT TTGTCTGGGC CCCCTTTTTT TTTCACGGAG ATTTGTGTTA 
GTGGGGGACC ATCAGCAGCT TCCTCCCCTG GTGCTAAACC GTGAAGCAAG AGCTCTTGGC 
ATGAGTGAAA GCTTATTCAA GAGGCTGGAG CAGAATAAGA GTGCTGTTGT ACAGTTAACC 
GTGCAGTACA GAATGAACAG TAAAATTATG TCCTTAAGTA ATAAGCTGAC CTATGAGGGC 
AAGCTGGAGT GTGGATCAGA CAAAGTGGCC AATGCAGTGA TAAACCTACG TCACTTTAAA 
GATGTGAAGC TGGAACTGGA ATTTTATGCT GACTATTCTG ATAATCCTTG GTTGATGGGA 
GTATTTGAAC CCAACAATCC TGTTTGTTTC CTTAATACAG ACAAGGTTCC AGCGCCAGAA 
CAAGTTGAAA AAGGTGGTGT GAGCAATGTA ACAGAAGCCA AACTCATAGT TTTCCTAACC 
TCCATTTTTG TTAAGGCTGG ATGCAGTCCC TCTGATATTG GTATTATTGC ACCGTACAGG 
CAGCAATTAA AGATCATCAA TGATTTATTG GCACGTTCTA TTGGGATGGT CGAAGTTAAT 
ACAGTAGACA AATACCAAGG AAGGGACAAA AGTATTGTCC TAGTATCTTT TGTTAGAAGT 
AATAAGGATG GAACTGTTGG TGAACTCTTG AAAGATTGGC GACGTCTTAA TGTTGCTATA 
ACCAGAGCCA AACATAAACT GATTCTTCTG GGGTGTGTGC CCTCACTAAA TTGCTATCCT 
CCTTTGGAGA AGCTGCTTAA TCATTTAAAC TCAGAAAAAT TAATCATTGA TCTTCCATCA 
AGAGAACATG AAAGTCTTTG CCACATATTG GGTGACTTTC AAAGAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871288.1
CDS	171..2681
Translation	MHKNTSTDFPQMQLSLPSDNSKDNSTCNIEVVKPMDIEESIWSPRFGLKGKIDVTVGVKIHRGYKTKYKIMPLELKTGKESNSIEHRSQVVLYTLLSQERRADPEAGLLLYLKTGQMYPVPANHLDKRELLKLRNQMAFSLFHRISKSATRQKTQLASLPQIIEEEKTCKYCSQIGNCALYSRAVEQQMDCSSVPIVMLPKIEEETQHLKQTHLEYFSLWCLMLTLESQSKDNKKNHQNIWLMPASEMEKSGSCIGNLIRMEHVKIVCDGQYLHNFQCKHGAIPVTNLMAGDRVIVSGEERSLFALSRGYVKEINMTTVTCLLDRNLSVLPESTLFRLDQEEKNCDIDTPLGNLSKLMENTFVSKKLRDLIIDFREPQFISYLSSVLPHDAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPAIQQFTEQEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKIFDFCIVDEASQISQPICLGPLFFSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKSAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLRHFKDVKLELEFYADYSDNPWLMGVFEPNNPVCFLNTDKVPAPEQVEKGGVSNVTEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLARSIGMVEVNTVDKYQGRDKSIVLVSFVRSNKDGTVGELLKDWRRLNVAITRAKHKLILLGCVPSLNCYPPLEKLLNHLNSEKLIIDLPSREHESLCHILGDFQRE

Target ORF information:

RefSeq Version	XM_017015799.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015799.1

ORF Insert Sequence:

ATGCATAAAA ACACTTCGAC TGACTTCCCT CAGATGCAGC TCTCTCTGCC AAGTGATAAT 
AGTAAGGATA ATTCAACATG TAACATTGAA GTCGTGAAAC CAATGGATAT TGAAGAAAGC 
ATTTGGTCCC CTAGGTTTGG ATTGAAAGGC AAAATAGATG TTACAGTTGG TGTGAAAATA 
CATCGAGGGT ATAAAACAAA ATACAAGATA ATGCCGCTGG AACTTAAAAC TGGCAAAGAA 
TCAAATTCTA TTGAACACCG TAGTCAGGTT GTTCTGTACA CTCTACTAAG CCAAGAGAGA 
AGAGCTGATC CAGAGGCTGG CTTGCTTCTC TACCTCAAGA CTGGTCAGAT GTACCCTGTG 
CCTGCCAACC ATCTAGATAA AAGAGAATTA TTAAAGCTAA GAAACCAGAT GGCATTCTCA 
TTGTTTCACC GTATTAGCAA ATCTGCTACT AGACAGAAGA CACAGCTTGC TTCTTTGCCA 
CAAATAATTG AGGAAGAGAA AACTTGTAAA TATTGTTCAC AAATTGGCAA TTGTGCTCTT 
TATAGCAGAG CAGTTGAACA ACAGATGGAT TGTAGTTCAG TCCCAATTGT GATGCTGCCC 
AAAATAGAAG AAGAAACCCA GCATCTGAAG CAAACACACT TAGAATATTT CAGCCTTTGG 
TGTCTAATGT TAACCCTGGA GTCACAATCG AAGGATAATA AAAAGAATCA CCAAAATATC 
TGGCTAATGC CTGCTTCGGA AATGGAGAAG AGTGGCAGTT GCATTGGAAA CCTGATTAGA 
ATGGAACATG TAAAGATAGT TTGTGATGGG CAATATTTAC ATAATTTCCA ATGTAAACAT 
GGTGCCATAC CTGTCACAAA TCTAATGGCA GGTGACAGAG TTATTGTAAG TGGAGAAGAA 
AGGTCACTGT TTGCTTTGTC TAGAGGATAT GTGAAGGAGA TTAACATGAC AACAGTAACT 
TGTTTATTAG ACAGAAACTT GTCGGTCCTT CCAGAATCAA CTTTGTTCAG ATTAGACCAA 
GAAGAAAAAA ATTGTGATAT AGATACCCCA TTAGGAAATC TTTCCAAATT GATGGAAAAC 
ACGTTTGTCA GCAAAAAACT TCGAGATTTA ATTATTGACT TTCGTGAACC TCAGTTTATA 
TCCTACCTTA GTTCTGTTCT TCCACATGAT GCAAAGGATA CAGTTGCCTG CATTCTAAAG 
GGTTTGAATA AGCCTCAGAG GCAAGCGATG AAAAAGGTAC TTCTTTCAAA AGACTACACA 
CTCATCGTGG GTATGCCTGG GACAGGAAAA ACAACTACGA TATGTACTCT CGTAAGAATT 
CTCTACGCCT GTGGTTTTAG CGTTTTGTTG ACCAGCTATA CACACTCTGC TGTTGACAAT 
ATTCTTTTGA AGTTAGCCAA GTTTAAAATA GGATTTTTGC GTTTGGGTCA GATTCAGAAG 
GTTCATCCAG CTATCCAGCA ATTTACAGAG CAAGAAATTT GCAGATCAAA GTCCATTAAA 
TCCTTAGCTC TTCTAGAAGA ACTCTACAAT AGTCAACTTA TAGTTGCAAC AACATGTATG 
GGAATAAACC ATCCAATATT TTCCCGTAAA ATTTTTGATT TTTGTATTGT GGATGAAGCC 
TCTCAAATTA GCCAACCAAT TTGTCTGGGC CCCCTTTTTT TTTCACGGAG ATTTGTGTTA 
GTGGGGGACC ATCAGCAGCT TCCTCCCCTG GTGCTAAACC GTGAAGCAAG AGCTCTTGGC 
ATGAGTGAAA GCTTATTCAA GAGGCTGGAG CAGAATAAGA GTGCTGTTGT ACAGTTAACC 
GTGCAGTACA GAATGAACAG TAAAATTATG TCCTTAAGTA ATAAGCTGAC CTATGAGGGC 
AAGCTGGAGT GTGGATCAGA CAAAGTGGCC AATGCAGTGA TAAACCTACG TCACTTTAAA 
GATGTGAAGC TGGAACTGGA ATTTTATGCT GACTATTCTG ATAATCCTTG GTTGATGGGA 
GTATTTGAAC CCAACAATCC TGTTTGTTTC CTTAATACAG ACAAGGTTCC AGCGCCAGAA 
CAAGTTGAAA AAGGTGGTGT GAGCAATGTA ACAGAAGCCA AACTCATAGT TTTCCTAACC 
TCCATTTTTG TTAAGGCTGG ATGCAGTCCC TCTGATATTG GTATTATTGC ACCGTACAGG 
CAGCAATTAA AGATCATCAA TGATTTATTG GCACGTTCTA TTGGGATGGT CGAAGTTAAT 
ACAGTAGACA AATACCAAGG AAGGGACAAA AGTATTGTCC TAGTATCTTT TGTTAGAAGT 
AATAAGGATG GAACTGTTGG TGAACTCTTG AAAGATTGGC GACGTCTTAA TGTTGCTATA 
ACCAGAGCCA AACATAAACT GATTCTTCTG GGGTGTGTGC CCTCACTAAA TTGCTATCCT 
CCTTTGGAGA AGCTGCTTAA TCATTTAAAC TCAGAAAAAT TAATCATTGA TCTTCCATCA 
AGAGAACATG AAAGTCTTTG CCACATATTG GGTGACTTTC AAAGAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNA2 ( NM_001080449.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001073918.2 Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27848
Clone ID Related Accession (Same CDS sequence)	NM_001080449.2 , NM_001080449.3
Accession Version	NM_001080449.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	DNA replication ATP-dependent helicase/nuclease DNA2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC404138.1, D42046.1 and BC073945.1. This sequence is a reference standard in the RefSeqGene project. On Jan 29, 2011 this sequence version replaced NM_001080449.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D42046.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGC TGAACGAACT GGAGCTGCTG ATGGAGAAGA GTTTTTGGGA GGAGGCGGAG 
CTGCCGGCGG AGCTATTTCA GAAGAAAGTG GTAGCTTCCT TTCCAAGAAC AGTTCTGAGC 
ACAGGAATGG ATAACCGGTA CCTGGTGTTG GCAGTCAATA CTGTACAGAA CAAAGAGGGA 
AACTGTGAAA AGCGCCTGGT CATCACTGCT TCACAGTCAC TAGAAAATAA AGAACTATGC 
ATCCTTAGGA ATGACTGGTG TTCTGTTCCA GTAGAGCCAG GAGATATCAT TCATTTGGAG 
GGAGACTGCA CATCTGACAC TTGGATAATA GATAAAGATT TTGGATATTT GATTCTGTAT 
CCAGACATGC TGATTTCTGG CACCAGCATA GCCAGTAGTA TTCGATGTAT GAGAAGAGCT 
GTCCTGAGTG AAACTTTTAG GAGCTCTGAT CCAGCCACAC GCCAAATGCT AATTGGTACG 
GTTCTCCATG AGGTGTTTCA AAAAGCCATA AATAATAGCT TTGCCCCAGA AAAGCTACAA 
GAACTTGCTT TTCAAACAAT TCAAGAAATA AGACATTTGA AGGAAATGTA CCGCTTAAAT 
CTAAGTCAAG ATGAAATAAA ACAAGAAGTA GAGGACTATC TTCCTTCGTT TTGTAAATGG 
GCAGGAGATT TCATGCATAA AAACACTTCG ACTGACTTCC CTCAGATGCA GCTCTCTCTG 
CCAAGTGATA ATAGTAAGGA TAATTCAACA TGTAACATTG AAGTCGTGAA ACCAATGGAT 
ATTGAAGAAA GCATTTGGTC CCCTAGGTTT GGATTGAAAG GCAAAATAGA TGTTACAGTT 
GGTGTGAAAA TACATCGAGG GTATAAAACA AAATACAAGA TAATGCCGCT GGAACTTAAA 
ACTGGCAAAG AATCAAATTC TATTGAACAC CGTAGTCAGG TTGTTCTGTA CACTCTACTA 
AGCCAAGAGA GAAGAGCTGA TCCAGAGGCT GGCTTGCTTC TCTACCTCAA GACTGGTCAG 
ATGTACCCTG TGCCTGCCAA CCATCTAGAT AAAAGAGAAT TATTAAAGCT AAGAAACCAG 
ATGGCATTCT CATTGTTTCA CCGTATTAGC AAATCTGCTA CTAGACAGAA GACACAGCTT 
GCTTCTTTGC CACAAATAAT TGAGGAAGAG AAAACTTGTA AATATTGTTC ACAAATTGGC 
AATTGTGCTC TTTATAGCAG AGCAGTTGAA CAACAGATGG ATTGTAGTTC AGTCCCAATT 
GTGATGCTGC CCAAAATAGA AGAAGAAACC CAGCATCTGA AGCAAACACA CTTAGAATAT 
TTCAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCACAAT CGAAGGATAA TAAAAAGAAT 
CACCAAAATA TCTGGCTAAT GCCTGCTTCG GAAATGGAGA AGAGTGGCAG TTGCATTGGA 
AACCTGATTA GAATGGAACA TGTAAAGATA GTTTGTGATG GGCAATATTT ACATAATTTC 
CAATGTAAAC ATGGTGCCAT ACCTGTCACA AATCTAATGG CAGGTGACAG AGTTATTGTA 
AGTGGAGAAG AAAGGTCACT GTTTGCTTTG TCTAGAGGAT ATGTGAAGGA GATTAACATG 
ACAACAGTAA CTTGTTTATT AGACAGAAAC TTGTCGGTCC TTCCAGAATC AACTTTGTTC 
AGATTAGACC AAGAAGAAAA AAATTGTGAT ATAGATACCC CATTAGGAAA TCTTTCCAAA 
TTGATGGAAA ACACGTTTGT CAGCAAAAAA CTTCGAGATT TAATTATTGA CTTTCGTGAA 
CCTCAGTTTA TATCCTACCT TAGTTCTGTT CTTCCACATG ATGCAAAGGA TACAGTTGCC 
TGCATTCTAA AGGGTTTGAA TAAGCCTCAG AGGCAAGCGA TGAAAAAGGT ACTTCTTTCA 
AAAGACTACA CACTCATCGT GGGTATGCCT GGGACAGGAA AAACAACTAC GATATGTACT 
CTCGTAAGAA TTCTCTACGC CTGTGGTTTT AGCGTTTTGT TGACCAGCTA TACACACTCT 
GCTGTTGACA ATATTCTTTT GAAGTTAGCC AAGTTTAAAA TAGGATTTTT GCGTTTGGGT 
CAGATTCAGA AGGTTCATCC AGCTATCCAG CAATTTACAG AGCAAGAAAT TTGCAGATCA 
AAGTCCATTA AATCCTTAGC TCTTCTAGAA GAACTCTACA ATAGTCAACT TATAGTTGCA 
ACAACATGTA TGGGAATAAA CCATCCAATA TTTTCCCGTA AAATTTTTGA TTTTTGTATT 
GTGGATGAAG CCTCTCAAAT TAGCCAACCA ATTTGTCTGG GCCCCCTTTT TTTTTCACGG 
AGATTTGTGT TAGTGGGGGA CCATCAGCAG CTTCCTCCCC TGGTGCTAAA CCGTGAAGCA 
AGAGCTCTTG GCATGAGTGA AAGCTTATTC AAGAGGCTGG AGCAGAATAA GAGTGCTGTT 
GTACAGTTAA CCGTGCAGTA CAGAATGAAC AGTAAAATTA TGTCCTTAAG TAATAAGCTG 
ACCTATGAGG GCAAGCTGGA GTGTGGATCA GACAAAGTGG CCAATGCAGT GATAAACCTA 
CGTCACTTTA AAGATGTGAA GCTGGAACTG GAATTTTATG CTGACTATTC TGATAATCCT 
TGGTTGATGG GAGTATTTGA ACCCAACAAT CCTGTTTGTT TCCTTAATAC AGACAAGGTT 
CCAGCGCCAG AACAAGTTGA AAAAGGTGGT GTGAGCAATG TAACAGAAGC CAAACTCATA 
GTTTTCCTAA CCTCCATTTT TGTTAAGGCT GGATGCAGTC CCTCTGATAT TGGTATTATT 
GCACCGTACA GGCAGCAATT AAAGATCATC AATGATTTAT TGGCACGTTC TATTGGGATG 
GTCGAAGTTA ATACAGTAGA CAAATACCAA GGAAGGGACA AAAGTATTGT CCTAGTATCT 
TTTGTTAGAA GTAATAAGGA TGGAACTGTT GGTGAACTCT TGAAAGATTG GCGACGTCTT 
AATGTTGCTA TAACCAGAGC CAAACATAAA CTGATTCTTC TGGGGTGTGT GCCCTCACTA 
AATTGCTATC CTCCTTTGGA GAAGCTGCTT AATCATTTAA ACTCAGAAAA ATTAATCATT 
GATCTTCCAT CAAGAGAACA TGAAAGTCTT TGCCACATAT TGGGTGACTT TCAAAGAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073918.2
CDS	110..3292
Translation	MEQLNELELLMEKSFWEEAELPAELFQKKVVASFPRTVLSTGMDNRYLVLAVNTVQNKEGNCEKRLVITASQSLENKELCILRNDWCSVPVEPGDIIHLEGDCTSDTWIIDKDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRSSDPATRQMLIGTVLHEVFQKAINNSFAPEKLQELAFQTIQEIRHLKEMYRLNLSQDEIKQEVEDYLPSFCKWAGDFMHKNTSTDFPQMQLSLPSDNSKDNSTCNIEVVKPMDIEESIWSPRFGLKGKIDVTVGVKIHRGYKTKYKIMPLELKTGKESNSIEHRSQVVLYTLLSQERRADPEAGLLLYLKTGQMYPVPANHLDKRELLKLRNQMAFSLFHRISKSATRQKTQLASLPQIIEEEKTCKYCSQIGNCALYSRAVEQQMDCSSVPIVMLPKIEEETQHLKQTHLEYFSLWCLMLTLESQSKDNKKNHQNIWLMPASEMEKSGSCIGNLIRMEHVKIVCDGQYLHNFQCKHGAIPVTNLMAGDRVIVSGEERSLFALSRGYVKEINMTTVTCLLDRNLSVLPESTLFRLDQEEKNCDIDTPLGNLSKLMENTFVSKKLRDLIIDFREPQFISYLSSVLPHDAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPAIQQFTEQEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKIFDFCIVDEASQISQPICLGPLFFSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKSAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLRHFKDVKLELEFYADYSDNPWLMGVFEPNNPVCFLNTDKVPAPEQVEKGGVSNVTEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLARSIGMVEVNTVDKYQGRDKSIVLVSFVRSNKDGTVGELLKDWRRLNVAITRAKHKLILLGCVPSLNCYPPLEKLLNHLNSEKLIIDLPSREHESLCHILGDFQRE

Target ORF information:

RefSeq Version	NM_001080449.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080449.2

ORF Insert Sequence:

ATGGAGCAGC TGAACGAACT GGAGCTGCTG ATGGAGAAGA GTTTTTGGGA GGAGGCGGAG 
CTGCCGGCGG AGCTATTTCA GAAGAAAGTG GTAGCTTCCT TTCCAAGAAC AGTTCTGAGC 
ACAGGAATGG ATAACCGGTA CCTGGTGTTG GCAGTCAATA CTGTACAGAA CAAAGAGGGA 
AACTGTGAAA AGCGCCTGGT CATCACTGCT TCACAGTCAC TAGAAAATAA AGAACTATGC 
ATCCTTAGGA ATGACTGGTG TTCTGTTCCA GTAGAGCCAG GAGATATCAT TCATTTGGAG 
GGAGACTGCA CATCTGACAC TTGGATAATA GATAAAGATT TTGGATATTT GATTCTGTAT 
CCAGACATGC TGATTTCTGG CACCAGCATA GCCAGTAGTA TTCGATGTAT GAGAAGAGCT 
GTCCTGAGTG AAACTTTTAG GAGCTCTGAT CCAGCCACAC GCCAAATGCT AATTGGTACG 
GTTCTCCATG AGGTGTTTCA AAAAGCCATA AATAATAGCT TTGCCCCAGA AAAGCTACAA 
GAACTTGCTT TTCAAACAAT TCAAGAAATA AGACATTTGA AGGAAATGTA CCGCTTAAAT 
CTAAGTCAAG ATGAAATAAA ACAAGAAGTA GAGGACTATC TTCCTTCGTT TTGTAAATGG 
GCAGGAGATT TCATGCATAA AAACACTTCG ACTGACTTCC CTCAGATGCA GCTCTCTCTG 
CCAAGTGATA ATAGTAAGGA TAATTCAACA TGTAACATTG AAGTCGTGAA ACCAATGGAT 
ATTGAAGAAA GCATTTGGTC CCCTAGGTTT GGATTGAAAG GCAAAATAGA TGTTACAGTT 
GGTGTGAAAA TACATCGAGG GTATAAAACA AAATACAAGA TAATGCCGCT GGAACTTAAA 
ACTGGCAAAG AATCAAATTC TATTGAACAC CGTAGTCAGG TTGTTCTGTA CACTCTACTA 
AGCCAAGAGA GAAGAGCTGA TCCAGAGGCT GGCTTGCTTC TCTACCTCAA GACTGGTCAG 
ATGTACCCTG TGCCTGCCAA CCATCTAGAT AAAAGAGAAT TATTAAAGCT AAGAAACCAG 
ATGGCATTCT CATTGTTTCA CCGTATTAGC AAATCTGCTA CTAGACAGAA GACACAGCTT 
GCTTCTTTGC CACAAATAAT TGAGGAAGAG AAAACTTGTA AATATTGTTC ACAAATTGGC 
AATTGTGCTC TTTATAGCAG AGCAGTTGAA CAACAGATGG ATTGTAGTTC AGTCCCAATT 
GTGATGCTGC CCAAAATAGA AGAAGAAACC CAGCATCTGA AGCAAACACA CTTAGAATAT 
TTCAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCACAAT CGAAGGATAA TAAAAAGAAT 
CACCAAAATA TCTGGCTAAT GCCTGCTTCG GAAATGGAGA AGAGTGGCAG TTGCATTGGA 
AACCTGATTA GAATGGAACA TGTAAAGATA GTTTGTGATG GGCAATATTT ACATAATTTC 
CAATGTAAAC ATGGTGCCAT ACCTGTCACA AATCTAATGG CAGGTGACAG AGTTATTGTA 
AGTGGAGAAG AAAGGTCACT GTTTGCTTTG TCTAGAGGAT ATGTGAAGGA GATTAACATG 
ACAACAGTAA CTTGTTTATT AGACAGAAAC TTGTCGGTCC TTCCAGAATC AACTTTGTTC 
AGATTAGACC AAGAAGAAAA AAATTGTGAT ATAGATACCC CATTAGGAAA TCTTTCCAAA 
TTGATGGAAA ACACGTTTGT CAGCAAAAAA CTTCGAGATT TAATTATTGA CTTTCGTGAA 
CCTCAGTTTA TATCCTACCT TAGTTCTGTT CTTCCACATG ATGCAAAGGA TACAGTTGCC 
TGCATTCTAA AGGGTTTGAA TAAGCCTCAG AGGCAAGCGA TGAAAAAGGT ACTTCTTTCA 
AAAGACTACA CACTCATCGT GGGTATGCCT GGGACAGGAA AAACAACTAC GATATGTACT 
CTCGTAAGAA TTCTCTACGC CTGTGGTTTT AGCGTTTTGT TGACCAGCTA TACACACTCT 
GCTGTTGACA ATATTCTTTT GAAGTTAGCC AAGTTTAAAA TAGGATTTTT GCGTTTGGGT 
CAGATTCAGA AGGTTCATCC AGCTATCCAG CAATTTACAG AGCAAGAAAT TTGCAGATCA 
AAGTCCATTA AATCCTTAGC TCTTCTAGAA GAACTCTACA ATAGTCAACT TATAGTTGCA 
ACAACATGTA TGGGAATAAA CCATCCAATA TTTTCCCGTA AAATTTTTGA TTTTTGTATT 
GTGGATGAAG CCTCTCAAAT TAGCCAACCA ATTTGTCTGG GCCCCCTTTT TTTTTCACGG 
AGATTTGTGT TAGTGGGGGA CCATCAGCAG CTTCCTCCCC TGGTGCTAAA CCGTGAAGCA 
AGAGCTCTTG GCATGAGTGA AAGCTTATTC AAGAGGCTGG AGCAGAATAA GAGTGCTGTT 
GTACAGTTAA CCGTGCAGTA CAGAATGAAC AGTAAAATTA TGTCCTTAAG TAATAAGCTG 
ACCTATGAGG GCAAGCTGGA GTGTGGATCA GACAAAGTGG CCAATGCAGT GATAAACCTA 
CGTCACTTTA AAGATGTGAA GCTGGAACTG GAATTTTATG CTGACTATTC TGATAATCCT 
TGGTTGATGG GAGTATTTGA ACCCAACAAT CCTGTTTGTT TCCTTAATAC AGACAAGGTT 
CCAGCGCCAG AACAAGTTGA AAAAGGTGGT GTGAGCAATG TAACAGAAGC CAAACTCATA 
GTTTTCCTAA CCTCCATTTT TGTTAAGGCT GGATGCAGTC CCTCTGATAT TGGTATTATT 
GCACCGTACA GGCAGCAATT AAAGATCATC AATGATTTAT TGGCACGTTC TATTGGGATG 
GTCGAAGTTA ATACAGTAGA CAAATACCAA GGAAGGGACA AAAGTATTGT CCTAGTATCT 
TTTGTTAGAA GTAATAAGGA TGGAACTGTT GGTGAACTCT TGAAAGATTG GCGACGTCTT 
AATGTTGCTA TAACCAGAGC CAAACATAAA CTGATTCTTC TGGGGTGTGT GCCCTCACTA 
AATTGCTATC CTCCTTTGGA GAAGCTGCTT AATCATTTAA ACTCAGAAAA ATTAATCATT 
GATCTTCCAT CAAGAGAACA TGAAAGTCTT TGCCACATAT TGGGTGACTT TCAAAGAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNA2 ( NM_001080449.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001073918.2 Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27848
Clone ID Related Accession (Same CDS sequence)	NM_001080449.2 , NM_001080449.3
Accession Version	NM_001080449.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	DNA replication ATP-dependent helicase/nuclease DNA2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC404138.1, D42046.1 and BC073945.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001080449.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D42046.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000358410.8/ ENSP00000351185.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCAGC TGAACGAACT GGAGCTGCTG ATGGAGAAGA GTTTTTGGGA GGAGGCGGAG 
CTGCCGGCGG AGCTATTTCA GAAGAAAGTG GTAGCTTCCT TTCCAAGAAC AGTTCTGAGC 
ACAGGAATGG ATAACCGGTA CCTGGTGTTG GCAGTCAATA CTGTACAGAA CAAAGAGGGA 
AACTGTGAAA AGCGCCTGGT CATCACTGCT TCACAGTCAC TAGAAAATAA AGAACTATGC 
ATCCTTAGGA ATGACTGGTG TTCTGTTCCA GTAGAGCCAG GAGATATCAT TCATTTGGAG 
GGAGACTGCA CATCTGACAC TTGGATAATA GATAAAGATT TTGGATATTT GATTCTGTAT 
CCAGACATGC TGATTTCTGG CACCAGCATA GCCAGTAGTA TTCGATGTAT GAGAAGAGCT 
GTCCTGAGTG AAACTTTTAG GAGCTCTGAT CCAGCCACAC GCCAAATGCT AATTGGTACG 
GTTCTCCATG AGGTGTTTCA AAAAGCCATA AATAATAGCT TTGCCCCAGA AAAGCTACAA 
GAACTTGCTT TTCAAACAAT TCAAGAAATA AGACATTTGA AGGAAATGTA CCGCTTAAAT 
CTAAGTCAAG ATGAAATAAA ACAAGAAGTA GAGGACTATC TTCCTTCGTT TTGTAAATGG 
GCAGGAGATT TCATGCATAA AAACACTTCG ACTGACTTCC CTCAGATGCA GCTCTCTCTG 
CCAAGTGATA ATAGTAAGGA TAATTCAACA TGTAACATTG AAGTCGTGAA ACCAATGGAT 
ATTGAAGAAA GCATTTGGTC CCCTAGGTTT GGATTGAAAG GCAAAATAGA TGTTACAGTT 
GGTGTGAAAA TACATCGAGG GTATAAAACA AAATACAAGA TAATGCCGCT GGAACTTAAA 
ACTGGCAAAG AATCAAATTC TATTGAACAC CGTAGTCAGG TTGTTCTGTA CACTCTACTA 
AGCCAAGAGA GAAGAGCTGA TCCAGAGGCT GGCTTGCTTC TCTACCTCAA GACTGGTCAG 
ATGTACCCTG TGCCTGCCAA CCATCTAGAT AAAAGAGAAT TATTAAAGCT AAGAAACCAG 
ATGGCATTCT CATTGTTTCA CCGTATTAGC AAATCTGCTA CTAGACAGAA GACACAGCTT 
GCTTCTTTGC CACAAATAAT TGAGGAAGAG AAAACTTGTA AATATTGTTC ACAAATTGGC 
AATTGTGCTC TTTATAGCAG AGCAGTTGAA CAACAGATGG ATTGTAGTTC AGTCCCAATT 
GTGATGCTGC CCAAAATAGA AGAAGAAACC CAGCATCTGA AGCAAACACA CTTAGAATAT 
TTCAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCACAAT CGAAGGATAA TAAAAAGAAT 
CACCAAAATA TCTGGCTAAT GCCTGCTTCG GAAATGGAGA AGAGTGGCAG TTGCATTGGA 
AACCTGATTA GAATGGAACA TGTAAAGATA GTTTGTGATG GGCAATATTT ACATAATTTC 
CAATGTAAAC ATGGTGCCAT ACCTGTCACA AATCTAATGG CAGGTGACAG AGTTATTGTA 
AGTGGAGAAG AAAGGTCACT GTTTGCTTTG TCTAGAGGAT ATGTGAAGGA GATTAACATG 
ACAACAGTAA CTTGTTTATT AGACAGAAAC TTGTCGGTCC TTCCAGAATC AACTTTGTTC 
AGATTAGACC AAGAAGAAAA AAATTGTGAT ATAGATACCC CATTAGGAAA TCTTTCCAAA 
TTGATGGAAA ACACGTTTGT CAGCAAAAAA CTTCGAGATT TAATTATTGA CTTTCGTGAA 
CCTCAGTTTA TATCCTACCT TAGTTCTGTT CTTCCACATG ATGCAAAGGA TACAGTTGCC 
TGCATTCTAA AGGGTTTGAA TAAGCCTCAG AGGCAAGCGA TGAAAAAGGT ACTTCTTTCA 
AAAGACTACA CACTCATCGT GGGTATGCCT GGGACAGGAA AAACAACTAC GATATGTACT 
CTCGTAAGAA TTCTCTACGC CTGTGGTTTT AGCGTTTTGT TGACCAGCTA TACACACTCT 
GCTGTTGACA ATATTCTTTT GAAGTTAGCC AAGTTTAAAA TAGGATTTTT GCGTTTGGGT 
CAGATTCAGA AGGTTCATCC AGCTATCCAG CAATTTACAG AGCAAGAAAT TTGCAGATCA 
AAGTCCATTA AATCCTTAGC TCTTCTAGAA GAACTCTACA ATAGTCAACT TATAGTTGCA 
ACAACATGTA TGGGAATAAA CCATCCAATA TTTTCCCGTA AAATTTTTGA TTTTTGTATT 
GTGGATGAAG CCTCTCAAAT TAGCCAACCA ATTTGTCTGG GCCCCCTTTT TTTTTCACGG 
AGATTTGTGT TAGTGGGGGA CCATCAGCAG CTTCCTCCCC TGGTGCTAAA CCGTGAAGCA 
AGAGCTCTTG GCATGAGTGA AAGCTTATTC AAGAGGCTGG AGCAGAATAA GAGTGCTGTT 
GTACAGTTAA CCGTGCAGTA CAGAATGAAC AGTAAAATTA TGTCCTTAAG TAATAAGCTG 
ACCTATGAGG GCAAGCTGGA GTGTGGATCA GACAAAGTGG CCAATGCAGT GATAAACCTA 
CGTCACTTTA AAGATGTGAA GCTGGAACTG GAATTTTATG CTGACTATTC TGATAATCCT 
TGGTTGATGG GAGTATTTGA ACCCAACAAT CCTGTTTGTT TCCTTAATAC AGACAAGGTT 
CCAGCGCCAG AACAAGTTGA AAAAGGTGGT GTGAGCAATG TAACAGAAGC CAAACTCATA 
GTTTTCCTAA CCTCCATTTT TGTTAAGGCT GGATGCAGTC CCTCTGATAT TGGTATTATT 
GCACCGTACA GGCAGCAATT AAAGATCATC AATGATTTAT TGGCACGTTC TATTGGGATG 
GTCGAAGTTA ATACAGTAGA CAAATACCAA GGAAGGGACA AAAGTATTGT CCTAGTATCT 
TTTGTTAGAA GTAATAAGGA TGGAACTGTT GGTGAACTCT TGAAAGATTG GCGACGTCTT 
AATGTTGCTA TAACCAGAGC CAAACATAAA CTGATTCTTC TGGGGTGTGT GCCCTCACTA 
AATTGCTATC CTCCTTTGGA GAAGCTGCTT AATCATTTAA ACTCAGAAAA ATTAATCATT 
GATCTTCCAT CAAGAGAACA TGAAAGTCTT TGCCACATAT TGGGTGACTT TCAAAGAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073918.2
CDS	110..3292
Translation	MEQLNELELLMEKSFWEEAELPAELFQKKVVASFPRTVLSTGMDNRYLVLAVNTVQNKEGNCEKRLVITASQSLENKELCILRNDWCSVPVEPGDIIHLEGDCTSDTWIIDKDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRSSDPATRQMLIGTVLHEVFQKAINNSFAPEKLQELAFQTIQEIRHLKEMYRLNLSQDEIKQEVEDYLPSFCKWAGDFMHKNTSTDFPQMQLSLPSDNSKDNSTCNIEVVKPMDIEESIWSPRFGLKGKIDVTVGVKIHRGYKTKYKIMPLELKTGKESNSIEHRSQVVLYTLLSQERRADPEAGLLLYLKTGQMYPVPANHLDKRELLKLRNQMAFSLFHRISKSATRQKTQLASLPQIIEEEKTCKYCSQIGNCALYSRAVEQQMDCSSVPIVMLPKIEEETQHLKQTHLEYFSLWCLMLTLESQSKDNKKNHQNIWLMPASEMEKSGSCIGNLIRMEHVKIVCDGQYLHNFQCKHGAIPVTNLMAGDRVIVSGEERSLFALSRGYVKEINMTTVTCLLDRNLSVLPESTLFRLDQEEKNCDIDTPLGNLSKLMENTFVSKKLRDLIIDFREPQFISYLSSVLPHDAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPAIQQFTEQEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKIFDFCIVDEASQISQPICLGPLFFSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKSAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLRHFKDVKLELEFYADYSDNPWLMGVFEPNNPVCFLNTDKVPAPEQVEKGGVSNVTEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLARSIGMVEVNTVDKYQGRDKSIVLVSFVRSNKDGTVGELLKDWRRLNVAITRAKHKLILLGCVPSLNCYPPLEKLLNHLNSEKLIIDLPSREHESLCHILGDFQRE

Target ORF information:

RefSeq Version	NM_001080449.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080449.3

ORF Insert Sequence:

ATGGAGCAGC TGAACGAACT GGAGCTGCTG ATGGAGAAGA GTTTTTGGGA GGAGGCGGAG 
CTGCCGGCGG AGCTATTTCA GAAGAAAGTG GTAGCTTCCT TTCCAAGAAC AGTTCTGAGC 
ACAGGAATGG ATAACCGGTA CCTGGTGTTG GCAGTCAATA CTGTACAGAA CAAAGAGGGA 
AACTGTGAAA AGCGCCTGGT CATCACTGCT TCACAGTCAC TAGAAAATAA AGAACTATGC 
ATCCTTAGGA ATGACTGGTG TTCTGTTCCA GTAGAGCCAG GAGATATCAT TCATTTGGAG 
GGAGACTGCA CATCTGACAC TTGGATAATA GATAAAGATT TTGGATATTT GATTCTGTAT 
CCAGACATGC TGATTTCTGG CACCAGCATA GCCAGTAGTA TTCGATGTAT GAGAAGAGCT 
GTCCTGAGTG AAACTTTTAG GAGCTCTGAT CCAGCCACAC GCCAAATGCT AATTGGTACG 
GTTCTCCATG AGGTGTTTCA AAAAGCCATA AATAATAGCT TTGCCCCAGA AAAGCTACAA 
GAACTTGCTT TTCAAACAAT TCAAGAAATA AGACATTTGA AGGAAATGTA CCGCTTAAAT 
CTAAGTCAAG ATGAAATAAA ACAAGAAGTA GAGGACTATC TTCCTTCGTT TTGTAAATGG 
GCAGGAGATT TCATGCATAA AAACACTTCG ACTGACTTCC CTCAGATGCA GCTCTCTCTG 
CCAAGTGATA ATAGTAAGGA TAATTCAACA TGTAACATTG AAGTCGTGAA ACCAATGGAT 
ATTGAAGAAA GCATTTGGTC CCCTAGGTTT GGATTGAAAG GCAAAATAGA TGTTACAGTT 
GGTGTGAAAA TACATCGAGG GTATAAAACA AAATACAAGA TAATGCCGCT GGAACTTAAA 
ACTGGCAAAG AATCAAATTC TATTGAACAC CGTAGTCAGG TTGTTCTGTA CACTCTACTA 
AGCCAAGAGA GAAGAGCTGA TCCAGAGGCT GGCTTGCTTC TCTACCTCAA GACTGGTCAG 
ATGTACCCTG TGCCTGCCAA CCATCTAGAT AAAAGAGAAT TATTAAAGCT AAGAAACCAG 
ATGGCATTCT CATTGTTTCA CCGTATTAGC AAATCTGCTA CTAGACAGAA GACACAGCTT 
GCTTCTTTGC CACAAATAAT TGAGGAAGAG AAAACTTGTA AATATTGTTC ACAAATTGGC 
AATTGTGCTC TTTATAGCAG AGCAGTTGAA CAACAGATGG ATTGTAGTTC AGTCCCAATT 
GTGATGCTGC CCAAAATAGA AGAAGAAACC CAGCATCTGA AGCAAACACA CTTAGAATAT 
TTCAGCCTTT GGTGTCTAAT GTTAACCCTG GAGTCACAAT CGAAGGATAA TAAAAAGAAT 
CACCAAAATA TCTGGCTAAT GCCTGCTTCG GAAATGGAGA AGAGTGGCAG TTGCATTGGA 
AACCTGATTA GAATGGAACA TGTAAAGATA GTTTGTGATG GGCAATATTT ACATAATTTC 
CAATGTAAAC ATGGTGCCAT ACCTGTCACA AATCTAATGG CAGGTGACAG AGTTATTGTA 
AGTGGAGAAG AAAGGTCACT GTTTGCTTTG TCTAGAGGAT ATGTGAAGGA GATTAACATG 
ACAACAGTAA CTTGTTTATT AGACAGAAAC TTGTCGGTCC TTCCAGAATC AACTTTGTTC 
AGATTAGACC AAGAAGAAAA AAATTGTGAT ATAGATACCC CATTAGGAAA TCTTTCCAAA 
TTGATGGAAA ACACGTTTGT CAGCAAAAAA CTTCGAGATT TAATTATTGA CTTTCGTGAA 
CCTCAGTTTA TATCCTACCT TAGTTCTGTT CTTCCACATG ATGCAAAGGA TACAGTTGCC 
TGCATTCTAA AGGGTTTGAA TAAGCCTCAG AGGCAAGCGA TGAAAAAGGT ACTTCTTTCA 
AAAGACTACA CACTCATCGT GGGTATGCCT GGGACAGGAA AAACAACTAC GATATGTACT 
CTCGTAAGAA TTCTCTACGC CTGTGGTTTT AGCGTTTTGT TGACCAGCTA TACACACTCT 
GCTGTTGACA ATATTCTTTT GAAGTTAGCC AAGTTTAAAA TAGGATTTTT GCGTTTGGGT 
CAGATTCAGA AGGTTCATCC AGCTATCCAG CAATTTACAG AGCAAGAAAT TTGCAGATCA 
AAGTCCATTA AATCCTTAGC TCTTCTAGAA GAACTCTACA ATAGTCAACT TATAGTTGCA 
ACAACATGTA TGGGAATAAA CCATCCAATA TTTTCCCGTA AAATTTTTGA TTTTTGTATT 
GTGGATGAAG CCTCTCAAAT TAGCCAACCA ATTTGTCTGG GCCCCCTTTT TTTTTCACGG 
AGATTTGTGT TAGTGGGGGA CCATCAGCAG CTTCCTCCCC TGGTGCTAAA CCGTGAAGCA 
AGAGCTCTTG GCATGAGTGA AAGCTTATTC AAGAGGCTGG AGCAGAATAA GAGTGCTGTT 
GTACAGTTAA CCGTGCAGTA CAGAATGAAC AGTAAAATTA TGTCCTTAAG TAATAAGCTG 
ACCTATGAGG GCAAGCTGGA GTGTGGATCA GACAAAGTGG CCAATGCAGT GATAAACCTA 
CGTCACTTTA AAGATGTGAA GCTGGAACTG GAATTTTATG CTGACTATTC TGATAATCCT 
TGGTTGATGG GAGTATTTGA ACCCAACAAT CCTGTTTGTT TCCTTAATAC AGACAAGGTT 
CCAGCGCCAG AACAAGTTGA AAAAGGTGGT GTGAGCAATG TAACAGAAGC CAAACTCATA 
GTTTTCCTAA CCTCCATTTT TGTTAAGGCT GGATGCAGTC CCTCTGATAT TGGTATTATT 
GCACCGTACA GGCAGCAATT AAAGATCATC AATGATTTAT TGGCACGTTC TATTGGGATG 
GTCGAAGTTA ATACAGTAGA CAAATACCAA GGAAGGGACA AAAGTATTGT CCTAGTATCT 
TTTGTTAGAA GTAATAAGGA TGGAACTGTT GGTGAACTCT TGAAAGATTG GCGACGTCTT 
AATGTTGCTA TAACCAGAGC CAAACATAAA CTGATTCTTC TGGGGTGTGT GCCCTCACTA 
AATTGCTATC CTCCTTTGGA GAAGCTGCTT AATCATTTAA ACTCAGAAAA ATTAATCATT 
GATCTTCCAT CAAGAGAACA TGAAAGTCTT TGCCACATAT TGGGTGACTT TCAAAGAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNA2 ( XM_006717680.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717743.1 Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant X1, mRNA.

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CloneID	OHu44095
Clone ID Related Accession (Same CDS sequence)	XM_006717680.3
Accession Version	XM_006717680.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717680.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTC CCTGTATTCC CAGTCCTAAG CAAGGGAGCA AAGGCAGGGG ACAGAGCCCT 
GCTGCTCAGT TTGCGATCCC CGCGTCCAGG ATGGAGCAGC TGAACGAACT GGAGCTGCTG 
ATGGAGAAGA GTTTTTGGGA GGAGGCGGAG CTGCCGGCGG AGCTATTTCA GAAGAAAGTG 
GTAGCTTCCT TTCCAAGAAC AGTTCTGAGC ACAGGAATGG ATAACCGGTA CCTGGTGTTG 
GCAGTCAATA CTGTACAGAA CAAAGAGGGA AACTGTGAAA AGCGCCTGGT CATCACTGCT 
TCACAGTCAC TAGAAAATAA AGAACTATGC ATCCTTAGGA ATGACTGGTG TTCTGTTCCA 
GTAGAGCCAG GAGATATCAT TCATTTGGAG GGAGACTGCA CATCTGACAC TTGGATAATA 
GATAAAGATT TTGGATATTT GATTCTGTAT CCAGACATGC TGATTTCTGG CACCAGCATA 
GCCAGTAGTA TTCGATGTAT GAGAAGAGCT GTCCTGAGTG AAACTTTTAG GAGCTCTGAT 
CCAGCCACAC GCCAAATGCT AATTGGTACG GTTCTCCATG AGGTGTTTCA AAAAGCCATA 
AATAATAGCT TTGCCCCAGA AAAGCTACAA GAACTTGCTT TTCAAACAAT TCAAGAAATA 
AGACATTTGA AGGAAATGTA CCGCTTAAAT CTAAGTCAAG ATGAAATAAA ACAAGAAGTA 
GAGGACTATC TTCCTTCGTT TTGTAAATGG GCAGGAGATT TCATGCATAA AAACACTTCG 
ACTGACTTCC CTCAGATGCA GCTCTCTCTG CCAAGTGATA ATAGTAAGGA TAATTCAACA 
TGTAACATTG AAGTCGTGAA ACCAATGGAT ATTGAAGAAA GCATTTGGTC CCCTAGGTTT 
GGATTGAAAG GCAAAATAGA TGTTACAGTT GGTGTGAAAA TACATCGAGG GTATAAAACA 
AAATACAAGA TAATGCCGCT GGAACTTAAA ACTGGCAAAG AATCAAATTC TATTGAACAC 
CGTAGTCAGG TTGTTCTGTA CACTCTACTA AGCCAAGAGA GAAGAGCTGA TCCAGAGGCT 
GGCTTGCTTC TCTACCTCAA GACTGGTCAG ATGTACCCTG TGCCTGCCAA CCATCTAGAT 
AAAAGAGAAT TATTAAAGCT AAGAAACCAG ATGGCATTCT CATTGTTTCA CCGTATTAGC 
AAATCTGCTA CTAGACAGAA GACACAGCTT GCTTCTTTGC CACAAATAAT TGAGGAAGAG 
AAAACTTGTA AATATTGTTC ACAAATTGGC AATTGTGCTC TTTATAGCAG AGCAGTTGAA 
CAACAGATGG ATTGTAGTTC AGTCCCAATT GTGATGCTGC CCAAAATAGA AGAAGAAACC 
CAGCATCTGA AGCAAACACA CTTAGAATAT TTCAGCCTTT GGTGTCTAAT GTTAACCCTG 
GAGTCACAAT CGAAGGATAA TAAAAAGAAT CACCAAAATA TCTGGCTAAT GCCTGCTTCG 
GAAATGGAGA AGAGTGGCAG TTGCATTGGA AACCTGATTA GAATGGAACA TGTAAAGATA 
GTTTGTGATG GGCAATATTT ACATAATTTC CAATGTAAAC ATGGTGCCAT ACCTGTCACA 
AATCTAATGG CAGGTGACAG AGTTATTGTA AGTGGAGAAG AAAGGTCACT GTTTGCTTTG 
TCTAGAGGAT ATGTGAAGGA GATTAACATG ACAACAGTAA CTTGTTTATT AGACAGAAAC 
TTGTCGGTCC TTCCAGAATC AACTTTGTTC AGATTAGACC AAGAAGAAAA AAATTGTGAT 
ATAGATACCC CATTAGGAAA TCTTTCCAAA TTGATGGAAA ACACGTTTGT CAGCAAAAAA 
CTTCGAGATT TAATTATTGA CTTTCGTGAA CCTCAGTTTA TATCCTACCT TAGTTCTGTT 
CTTCCACATG ATGCAAAGGA TACAGTTGCC TGCATTCTAA AGGGTTTGAA TAAGCCTCAG 
AGGCAAGCGA TGAAAAAGGT ACTTCTTTCA AAAGACTACA CACTCATCGT GGGTATGCCT 
GGGACAGGAA AAACAACTAC GATATGTACT CTCGTAAGAA TTCTCTACGC CTGTGGTTTT 
AGCGTTTTGT TGACCAGCTA TACACACTCT GCTGTTGACA ATATTCTTTT GAAGTTAGCC 
AAGTTTAAAA TAGGATTTTT GCGTTTGGGT CAGATTCAGA AGGTTCATCC AGCTATCCAG 
CAATTTACAG AGCAAGAAAT TTGCAGATCA AAGTCCATTA AATCCTTAGC TCTTCTAGAA 
GAACTCTACA ATAGTCAACT TATAGTTGCA ACAACATGTA TGGGAATAAA CCATCCAATA 
TTTTCCCGTA AAATTTTTGA TTTTTGTATT GTGGATGAAG CCTCTCAAAT TAGCCAACCA 
ATTTGTCTGG GCCCCCTTTT TTTTTCACGG AGATTTGTGT TAGTGGGGGA CCATCAGCAG 
CTTCCTCCCC TGGTGCTAAA CCGTGAAGCA AGAGCTCTTG GCATGAGTGA AAGCTTATTC 
AAGAGGCTGG AGCAGAATAA GAGTGCTGTT GTACAGTTAA CCGTGCAGTA CAGAATGAAC 
AGTAAAATTA TGTCCTTAAG TAATAAGCTG ACCTATGAGG GCAAGCTGGA GTGTGGATCA 
GACAAAGTGG CCAATGCAGT GATAAACCTA CGTCACTTTA AAGATGTGAA GCTGGAACTG 
GAATTTTATG CTGACTATTC TGATAATCCT TGGTTGATGG GAGTATTTGA ACCCAACAAT 
CCTGTTTGTT TCCTTAATAC AGACAAGGTT CCAGCGCCAG AACAAGTTGA AAAAGGTGGT 
GTGAGCAATG TAACAGAAGC CAAACTCATA GTTTTCCTAA CCTCCATTTT TGTTAAGGCT 
GGATGCAGTC CCTCTGATAT TGGTATTATT GCACCGTACA GGCAGCAATT AAAGATCATC 
AATGATTTAT TGGCACGTTC TATTGGGATG GTCGAAGTTA ATACAGTAGA CAAATACCAA 
GGAAGGGACA AAAGTATTGT CCTAGTATCT TTTGTTAGAA GTAATAAGGA TGGAACTGTT 
GGTGAACTCT TGAAAGATTG GCGACGTCTT AATGTTGCTA TAACCAGAGC CAAACATAAA 
CTGATTCTTC TGGGGTGTGT GCCCTCACTA AATTGCTATC CTCCTTTGGA GAAGCTGCTT 
AATCATTTAA ACTCAGAAAA ATTAATCATT GATCTTCCAT CAAGAGAACA TGAAAGTCTT 
TGCCACATAT TGGGTGACTT TCAAAGAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717743.1
CDS	400..3672
Translation	MKTPCIPSPKQGSKGRGQSPAAQFAIPASRMEQLNELELLMEKSFWEEAELPAELFQKKVVASFPRTVLSTGMDNRYLVLAVNTVQNKEGNCEKRLVITASQSLENKELCILRNDWCSVPVEPGDIIHLEGDCTSDTWIIDKDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRSSDPATRQMLIGTVLHEVFQKAINNSFAPEKLQELAFQTIQEIRHLKEMYRLNLSQDEIKQEVEDYLPSFCKWAGDFMHKNTSTDFPQMQLSLPSDNSKDNSTCNIEVVKPMDIEESIWSPRFGLKGKIDVTVGVKIHRGYKTKYKIMPLELKTGKESNSIEHRSQVVLYTLLSQERRADPEAGLLLYLKTGQMYPVPANHLDKRELLKLRNQMAFSLFHRISKSATRQKTQLASLPQIIEEEKTCKYCSQIGNCALYSRAVEQQMDCSSVPIVMLPKIEEETQHLKQTHLEYFSLWCLMLTLESQSKDNKKNHQNIWLMPASEMEKSGSCIGNLIRMEHVKIVCDGQYLHNFQCKHGAIPVTNLMAGDRVIVSGEERSLFALSRGYVKEINMTTVTCLLDRNLSVLPESTLFRLDQEEKNCDIDTPLGNLSKLMENTFVSKKLRDLIIDFREPQFISYLSSVLPHDAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPAIQQFTEQEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKIFDFCIVDEASQISQPICLGPLFFSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKSAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLRHFKDVKLELEFYADYSDNPWLMGVFEPNNPVCFLNTDKVPAPEQVEKGGVSNVTEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLARSIGMVEVNTVDKYQGRDKSIVLVSFVRSNKDGTVGELLKDWRRLNVAITRAKHKLILLGCVPSLNCYPPLEKLLNHLNSEKLIIDLPSREHESLCHILGDFQRE

Target ORF information:

RefSeq Version	XM_006717680.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA replication helicase/nuclease 2 (DNA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717680.3

ORF Insert Sequence:

ATGAAGACTC CCTGTATTCC CAGTCCTAAG CAAGGGAGCA AAGGCAGGGG ACAGAGCCCT 
GCTGCTCAGT TTGCGATCCC CGCGTCCAGG ATGGAGCAGC TGAACGAACT GGAGCTGCTG 
ATGGAGAAGA GTTTTTGGGA GGAGGCGGAG CTGCCGGCGG AGCTATTTCA GAAGAAAGTG 
GTAGCTTCCT TTCCAAGAAC AGTTCTGAGC ACAGGAATGG ATAACCGGTA CCTGGTGTTG 
GCAGTCAATA CTGTACAGAA CAAAGAGGGA AACTGTGAAA AGCGCCTGGT CATCACTGCT 
TCACAGTCAC TAGAAAATAA AGAACTATGC ATCCTTAGGA ATGACTGGTG TTCTGTTCCA 
GTAGAGCCAG GAGATATCAT TCATTTGGAG GGAGACTGCA CATCTGACAC TTGGATAATA 
GATAAAGATT TTGGATATTT GATTCTGTAT CCAGACATGC TGATTTCTGG CACCAGCATA 
GCCAGTAGTA TTCGATGTAT GAGAAGAGCT GTCCTGAGTG AAACTTTTAG GAGCTCTGAT 
CCAGCCACAC GCCAAATGCT AATTGGTACG GTTCTCCATG AGGTGTTTCA AAAAGCCATA 
AATAATAGCT TTGCCCCAGA AAAGCTACAA GAACTTGCTT TTCAAACAAT TCAAGAAATA 
AGACATTTGA AGGAAATGTA CCGCTTAAAT CTAAGTCAAG ATGAAATAAA ACAAGAAGTA 
GAGGACTATC TTCCTTCGTT TTGTAAATGG GCAGGAGATT TCATGCATAA AAACACTTCG 
ACTGACTTCC CTCAGATGCA GCTCTCTCTG CCAAGTGATA ATAGTAAGGA TAATTCAACA 
TGTAACATTG AAGTCGTGAA ACCAATGGAT ATTGAAGAAA GCATTTGGTC CCCTAGGTTT 
GGATTGAAAG GCAAAATAGA TGTTACAGTT GGTGTGAAAA TACATCGAGG GTATAAAACA 
AAATACAAGA TAATGCCGCT GGAACTTAAA ACTGGCAAAG AATCAAATTC TATTGAACAC 
CGTAGTCAGG TTGTTCTGTA CACTCTACTA AGCCAAGAGA GAAGAGCTGA TCCAGAGGCT 
GGCTTGCTTC TCTACCTCAA GACTGGTCAG ATGTACCCTG TGCCTGCCAA CCATCTAGAT 
AAAAGAGAAT TATTAAAGCT AAGAAACCAG ATGGCATTCT CATTGTTTCA CCGTATTAGC 
AAATCTGCTA CTAGACAGAA GACACAGCTT GCTTCTTTGC CACAAATAAT TGAGGAAGAG 
AAAACTTGTA AATATTGTTC ACAAATTGGC AATTGTGCTC TTTATAGCAG AGCAGTTGAA 
CAACAGATGG ATTGTAGTTC AGTCCCAATT GTGATGCTGC CCAAAATAGA AGAAGAAACC 
CAGCATCTGA AGCAAACACA CTTAGAATAT TTCAGCCTTT GGTGTCTAAT GTTAACCCTG 
GAGTCACAAT CGAAGGATAA TAAAAAGAAT CACCAAAATA TCTGGCTAAT GCCTGCTTCG 
GAAATGGAGA AGAGTGGCAG TTGCATTGGA AACCTGATTA GAATGGAACA TGTAAAGATA 
GTTTGTGATG GGCAATATTT ACATAATTTC CAATGTAAAC ATGGTGCCAT ACCTGTCACA 
AATCTAATGG CAGGTGACAG AGTTATTGTA AGTGGAGAAG AAAGGTCACT GTTTGCTTTG 
TCTAGAGGAT ATGTGAAGGA GATTAACATG ACAACAGTAA CTTGTTTATT AGACAGAAAC 
TTGTCGGTCC TTCCAGAATC AACTTTGTTC AGATTAGACC AAGAAGAAAA AAATTGTGAT 
ATAGATACCC CATTAGGAAA TCTTTCCAAA TTGATGGAAA ACACGTTTGT CAGCAAAAAA 
CTTCGAGATT TAATTATTGA CTTTCGTGAA CCTCAGTTTA TATCCTACCT TAGTTCTGTT 
CTTCCACATG ATGCAAAGGA TACAGTTGCC TGCATTCTAA AGGGTTTGAA TAAGCCTCAG 
AGGCAAGCGA TGAAAAAGGT ACTTCTTTCA AAAGACTACA CACTCATCGT GGGTATGCCT 
GGGACAGGAA AAACAACTAC GATATGTACT CTCGTAAGAA TTCTCTACGC CTGTGGTTTT 
AGCGTTTTGT TGACCAGCTA TACACACTCT GCTGTTGACA ATATTCTTTT GAAGTTAGCC 
AAGTTTAAAA TAGGATTTTT GCGTTTGGGT CAGATTCAGA AGGTTCATCC AGCTATCCAG 
CAATTTACAG AGCAAGAAAT TTGCAGATCA AAGTCCATTA AATCCTTAGC TCTTCTAGAA 
GAACTCTACA ATAGTCAACT TATAGTTGCA ACAACATGTA TGGGAATAAA CCATCCAATA 
TTTTCCCGTA AAATTTTTGA TTTTTGTATT GTGGATGAAG CCTCTCAAAT TAGCCAACCA 
ATTTGTCTGG GCCCCCTTTT TTTTTCACGG AGATTTGTGT TAGTGGGGGA CCATCAGCAG 
CTTCCTCCCC TGGTGCTAAA CCGTGAAGCA AGAGCTCTTG GCATGAGTGA AAGCTTATTC 
AAGAGGCTGG AGCAGAATAA GAGTGCTGTT GTACAGTTAA CCGTGCAGTA CAGAATGAAC 
AGTAAAATTA TGTCCTTAAG TAATAAGCTG ACCTATGAGG GCAAGCTGGA GTGTGGATCA 
GACAAAGTGG CCAATGCAGT GATAAACCTA CGTCACTTTA AAGATGTGAA GCTGGAACTG 
GAATTTTATG CTGACTATTC TGATAATCCT TGGTTGATGG GAGTATTTGA ACCCAACAAT 
CCTGTTTGTT TCCTTAATAC AGACAAGGTT CCAGCGCCAG AACAAGTTGA AAAAGGTGGT 
GTGAGCAATG TAACAGAAGC CAAACTCATA GTTTTCCTAA CCTCCATTTT TGTTAAGGCT 
GGATGCAGTC CCTCTGATAT TGGTATTATT GCACCGTACA GGCAGCAATT AAAGATCATC 
AATGATTTAT TGGCACGTTC TATTGGGATG GTCGAAGTTA ATACAGTAGA CAAATACCAA 
GGAAGGGACA AAAGTATTGT CCTAGTATCT TTTGTTAGAA GTAATAAGGA TGGAACTGTT 
GGTGAACTCT TGAAAGATTG GCGACGTCTT AATGTTGCTA TAACCAGAGC CAAACATAAA 
CTGATTCTTC TGGGGTGTGT GCCCTCACTA AATTGCTATC CTCCTTTGGA GAAGCTGCTT 
AATCATTTAA ACTCAGAAAA ATTAATCATT GATCTTCCAT CAAGAGAACA TGAAAGTCTT 
TGCCACATAT TGGGTGACTT TCAAAGAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of human DNA2 (hDNA2) as a potential target for cancer and other diseases: A systematic review.
DNA repair599-19(2017 Nov)
Jia PP,Junaid M,Ma YB,Ahmad F,Jia YF,Li WG,Pei DS

The motor activity of DNA2 functions as an ssDNA translocase to promote DNA end resection.
Genes & development31(5)493-502(2017 Mar)
Levikova M,Pinto C,Cejka P

A balance between elongation and trimming regulates telomere stability in stem cells.
Nature structural & molecular biology24(1)30-39(2017 Jan)
Rivera T,Haggblom C,Cosconati S,Karlseder J

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DNA2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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