SIRT3 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SIRT3
Entrez Gene ID	558775
Full Name	sirtuin 3
Synonyms	zgc:158774
General protein information	Preferred Names sirtuin 3 Names NAD-dependent protein deacetylase sirtuin-3, mitochondrial
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009303487.3	XP_009301762.1	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X1
XM_009303487.2	XP_009301762.1	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X1
NM_001080174.1	NP_001073643.1	NAD-dependent protein deacetylase sirtuin-3, mitochondrial
XM_009303488.3	XP_009301763.1	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X2
XM_017356877.2	XP_017212366.1	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X3
XM_009303488.2	XP_009301763.1	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X2
XM_017356877.1	XP_017212366.1	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SIRT3	XP_003312891.1
Mus musculus (house mouse)	Sirt3	NP_001171275.1
Xenopus tropicalis (tropical clawed frog)	sirt3	XP_002937698.2
Bos taurus (cattle)	SIRT3	NP_001193598.1
Gallus gallus (chicken)	SIRT3	NP_001186422.1
Danio rerio (zebrafish)	sirt3	NP_001073643.1
Homo sapiens (human)	SIRT3	NP_036371.1
	SIRT3	XP_001116486.2
Canis lupus familiaris (dog)	SIRT3	XP_855809.1
Rattus norvegicus (Norway rat)	Sirt3	NP_001099783.1

Gene Ontology
Bibliography

Related articles in PubMed

Altered Adipogenesis in Zebrafish Larvae Following High Fat Diet and Chemical Exposure Is Visualised by Stimulated Raman Scattering Microscopy.
den Broeder MJ, Moester MJB, Kamstra JH, Cenijn PH, Davidoiu V, Kamminga LM, Ariese F, de Boer JF, Legler J
International journal of molecular sciences18(4)(2017 Apr)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SIRT3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIRT3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02514	NM_001080174.1 Latest version!	Danio rerio sirtuin 3 (sirt3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa42910	XM_009303487.3 Latest version!	Danio rerio sirtuin 3 (sirt3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa42911	XM_009303488.3 Latest version!	Danio rerio sirtuin 3 (sirt3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa59637	XM_017356877.2 Latest version!	Danio rerio sirtuin 3 (sirt3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
ODa42910	XM_009303487.2	Danio rerio sirtuin 3 (sirt3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa42911	XM_009303488.2	Danio rerio sirtuin 3 (sirt3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa59637	XM_017356877.1	Danio rerio sirtuin 3 (sirt3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIRT3 ( NM_001080174.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001073643.1 Danio rerio sirtuin 3 (sirt3), mRNA.

Price & Availability↑

CloneID	ODa02514
Clone ID Related Accession (Same CDS sequence)	NM_001080174.1
Accession Version	NM_001080174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Danio rerio(zebrafish)
Product	NAD-dependent protein deacetylase sirtuin-3, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC129442.1. On Mar 11, 2007 this sequence version replaced XM_679133.2. ##Evidence-Data-START## Transcript exon combination :: BC129442.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCTGTACT TAAATACCTT TTTGCCAAGT GTTTGCAGAC GCTGTTTTGC AGAGAATCTC 
TTATGGAGAA GAGGATTGAC TACTACACAG AATCTGTCAA GAACAAAGCT CGTTCATCAA 
AAGACTCTGT CACATTTCCC TCATGCACAG AAGGGTGCTG CATTTCTTTC ACAGTTCATT 
TATTGCCCAG CTGCCTTCAT TAAATGTGGT GGAACAAGAG GCCTGTTTGG AGGAGGCCGT 
GACAATGTTC ACCAGCAAAC CCTTGAAGAC ATTGCAGAAA AAATCAGAGA ACGCAAGTTT 
AAACGAATTG TGGTGATGGC AGGGGCAGGC ATCAGCACAC CAAGTGGAAT CCCAGACTTC 
AGATCACCCG GCAGCGGCCT TTATGATAAC CTTCAACAGT ACAATCTACC CTATGCTGAA 
GCCATATTTG AAATAAATTA TTTCCATCAT AATCCTAACC CTTTTTTCGC TCTTGCCAAA 
GAGCTTTATC CTGGTAATTA CCAACCCAAC CTGACGCACT ACTTCATTCG CATGCTTCAC 
GATAAGGAAC AGCTGCTCAG GATGTACACG CAAAACATTG ATGGACTTGA GCGCATGGCT 
GGCATTCCTC CAAAGATGCT TGTGGAGGCA CATGGAACAT TTGCCACTGC CACATGCACA 
GTTTGTCGGA GGGATTACAA AGGAGAGGAA CTTCGGGATG ACATCATGGC AGGAACAGTA 
CCCAAATGCC CAACTTGTAA AGGAATCATC AAGCCTGACA TTGTGTTCTT TGGTGAGGAG 
CTTCCACAGC ACTTCTTCAC CTACCTTACA GACTTCCCAA TAGCAGACCT GCTCATTGTG 
ATGGGAACAT CTCTTGAGGT GGAGCCCTTT GCAAGTTTGG CTGGTGCTGT GCGTGGTTCT 
GTACCAAGGC TTTTGATAAA TCGAGACCTT GTGGGGCCGT TTGCCTCGGG GTCCCAACGT 
CACACTGACG TGGCTGAGCT GGGTGACGTG GTTAATGGGG TAAAAAAGCT GGTGGAGCTC 
CTGGGCTGGA AGCAGGAATT AGAAGACTTA ATGAATGTTG GCAGAGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073643.1
CDS	67..1140
Translation	MLYLNTFLPSVCRRCFAENLLWRRGLTTTQNLSRTKLVHQKTLSHFPHAQKGAAFLSQFIYCPAAFIKCGGTRGLFGGGRDNVHQQTLEDIAEKIRERKFKRIVVMAGAGISTPSGIPDFRSPGSGLYDNLQQYNLPYAEAIFEINYFHHNPNPFFALAKELYPGNYQPNLTHYFIRMLHDKEQLLRMYTQNIDGLERMAGIPPKMLVEAHGTFATATCTVCRRDYKGEELRDDIMAGTVPKCPTCKGIIKPDIVFFGEELPQHFFTYLTDFPIADLLIVMGTSLEVEPFASLAGAVRGSVPRLLINRDLVGPFASGSQRHTDVAELGDVVNGVKKLVELLGWKQELEDLMNVGRDK

Target ORF information:

RefSeq Version	NM_001080174.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sirtuin 3 (sirt3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080174.1

ORF Insert Sequence:

ATGCTGTACT TAAATACCTT TTTGCCAAGT GTTTGCAGAC GCTGTTTTGC AGAGAATCTC 
TTATGGAGAA GAGGATTGAC TACTACACAG AATCTGTCAA GAACAAAGCT CGTTCATCAA 
AAGACTCTGT CACATTTCCC TCATGCACAG AAGGGTGCTG CATTTCTTTC ACAGTTCATT 
TATTGCCCAG CTGCCTTCAT TAAATGTGGT GGAACAAGAG GCCTGTTTGG AGGAGGCCGT 
GACAATGTTC ACCAGCAAAC CCTTGAAGAC ATTGCAGAAA AAATCAGAGA ACGCAAGTTT 
AAACGAATTG TGGTGATGGC AGGGGCAGGC ATCAGCACAC CAAGTGGAAT CCCAGACTTC 
AGATCACCCG GCAGCGGCCT TTATGATAAC CTTCAACAGT ACAATCTACC CTATGCTGAA 
GCCATATTTG AAATAAATTA TTTCCATCAT AATCCTAACC CTTTTTTCGC TCTTGCCAAA 
GAGCTTTATC CTGGTAATTA CCAACCCAAC CTGACGCACT ACTTCATTCG CATGCTTCAC 
GATAAGGAAC AGCTGCTCAG GATGTACACG CAAAACATTG ATGGACTTGA GCGCATGGCT 
GGCATTCCTC CAAAGATGCT TGTGGAGGCA CATGGAACAT TTGCCACTGC CACATGCACA 
GTTTGTCGGA GGGATTACAA AGGAGAGGAA CTTCGGGATG ACATCATGGC AGGAACAGTA 
CCCAAATGCC CAACTTGTAA AGGAATCATC AAGCCTGACA TTGTGTTCTT TGGTGAGGAG 
CTTCCACAGC ACTTCTTCAC CTACCTTACA GACTTCCCAA TAGCAGACCT GCTCATTGTG 
ATGGGAACAT CTCTTGAGGT GGAGCCCTTT GCAAGTTTGG CTGGTGCTGT GCGTGGTTCT 
GTACCAAGGC TTTTGATAAA TCGAGACCTT GTGGGGCCGT TTGCCTCGGG GTCCCAACGT 
CACACTGACG TGGCTGAGCT GGGTGACGTG GTTAATGGGG TAAAAAAGCT GGTGGAGCTC 
CTGGGCTGGA AGCAGGAATT AGAAGACTTA ATGAATGTTG GCAGAGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT3 ( XM_009303487.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301762.1 Danio rerio sirtuin 3 (sirt3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42910
Clone ID Related Accession (Same CDS sequence)	XM_009303487.3 , XM_009303487.2
Accession Version	XM_009303487.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303487.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTACT TAAATACCTT TTTGCCAAGT GTTTGCAGAC GCTGTTTTGC AGAGAATCTC 
TTATGGAGAA GAGGATTGAC TACTACACAG AATCTGTCAA GAACAAAGCT CGTTCATCAA 
AAGACTCTGT CACATTTCCC TCATGCACAG AAGGGTGCTG CATTTCTTTC ACAGTTCATT 
TATTGCCCAG CTGCCTTCAT TAAATGTGGT GGAACAAGAG GCCTGTTTGG AGGAGGCCGT 
GACAATGTTC ACCAGCAAAC CCTTGAAGAC ATTGCAGAAA AAATCAGAGA ACGCAAGTTT 
AAACGAATTG TGGTGATGGC AGGGGCAGGC ATCAGCACAC CAAGTGGAAT CCCAGACTTC 
AGATCACCCG GCAGCGGCCT TTATGATAAC CTTCAACAGT ACAATCTACC CTATGCTGAA 
GCCATATTTG AAATAAATTA TTTCCATCAT AATCCTAACC CTTTTTTCGC TCTTGCCAAA 
GAGCTTTATC CTGGTAATTA CCAACCCAAC CTGACGCACT ACTTCATTCG CATGCTTCAC 
GATAAGGAAC AGCTGCTCAG GATGTACACG CAAAACATTG ATGGACTTGA GCGCATGGCT 
GGCATTCCTC CAAAGATGCT TGTGGAGGCA CATGGAACAT TTGCCACTGC CACATGCACA 
GTTTGTCGGA GGGATTACAA AGGAGAGGAA CTTCGGGATG ACATCATGGC AGGAACAGTA 
CCCAAATGCC CAACTTGTAA AGGAATCATC AAGCCTGACA TTGTGTTCTT TGGTGAGGAG 
CTTCCACAGC ACTTCTTCAC CTACCTTACA GACTTCCCAA TAGCAGACCT GCTCATTGTG 
ATGGGAACAT CTCTTGAGGT GGAGCCCTTT GCAAGTTTGG CTGGTGCTGT GCGTGGTTCT 
GTACCAAGGC TTTTGATAAA TCGAGACCTT GTGGGGCCGT TTGCCTCGGG GTCCCAACGT 
CACACTGACG TGGCTGAGCT GGGTGACGTG GTTAATGGGG TAAAAAAGCT GGTGGAGCTC 
CTGGGCTGGA AGCAGGAATT AGAAGACTTA ATGAATGTTG GCAGAGACAA GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301762.1
CDS	102..1178
Translation	MLYLNTFLPSVCRRCFAENLLWRRGLTTTQNLSRTKLVHQKTLSHFPHAQKGAAFLSQFIYCPAAFIKCGGTRGLFGGGRDNVHQQTLEDIAEKIRERKFKRIVVMAGAGISTPSGIPDFRSPGSGLYDNLQQYNLPYAEAIFEINYFHHNPNPFFALAKELYPGNYQPNLTHYFIRMLHDKEQLLRMYTQNIDGLERMAGIPPKMLVEAHGTFATATCTVCRRDYKGEELRDDIMAGTVPKCPTCKGIIKPDIVFFGEELPQHFFTYLTDFPIADLLIVMGTSLEVEPFASLAGAVRGSVPRLLINRDLVGPFASGSQRHTDVAELGDVVNGVKKLVELLGWKQELEDLMNVGRDKV

Target ORF information:

RefSeq Version	XM_009303487.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sirtuin 3 (sirt3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303487.3

ORF Insert Sequence:

ATGCTGTACT TAAATACCTT TTTGCCAAGT GTTTGCAGAC GCTGTTTTGC AGAGAATCTC 
TTATGGAGAA GAGGATTGAC TACTACACAG AATCTGTCAA GAACAAAGCT CGTTCATCAA 
AAGACTCTGT CACATTTCCC TCATGCACAG AAGGGTGCTG CATTTCTTTC ACAGTTCATT 
TATTGCCCAG CTGCCTTCAT TAAATGTGGT GGAACAAGAG GCCTGTTTGG AGGAGGCCGT 
GACAATGTTC ACCAGCAAAC CCTTGAAGAC ATTGCAGAAA AAATCAGAGA ACGCAAGTTT 
AAACGAATTG TGGTGATGGC AGGGGCAGGC ATCAGCACAC CAAGTGGAAT CCCAGACTTC 
AGATCACCCG GCAGCGGCCT TTATGATAAC CTTCAACAGT ACAATCTACC CTATGCTGAA 
GCCATATTTG AAATAAATTA TTTCCATCAT AATCCTAACC CTTTTTTCGC TCTTGCCAAA 
GAGCTTTATC CTGGTAATTA CCAACCCAAC CTGACGCACT ACTTCATTCG CATGCTTCAC 
GATAAGGAAC AGCTGCTCAG GATGTACACG CAAAACATTG ATGGACTTGA GCGCATGGCT 
GGCATTCCTC CAAAGATGCT TGTGGAGGCA CATGGAACAT TTGCCACTGC CACATGCACA 
GTTTGTCGGA GGGATTACAA AGGAGAGGAA CTTCGGGATG ACATCATGGC AGGAACAGTA 
CCCAAATGCC CAACTTGTAA AGGAATCATC AAGCCTGACA TTGTGTTCTT TGGTGAGGAG 
CTTCCACAGC ACTTCTTCAC CTACCTTACA GACTTCCCAA TAGCAGACCT GCTCATTGTG 
ATGGGAACAT CTCTTGAGGT GGAGCCCTTT GCAAGTTTGG CTGGTGCTGT GCGTGGTTCT 
GTACCAAGGC TTTTGATAAA TCGAGACCTT GTGGGGCCGT TTGCCTCGGG GTCCCAACGT 
CACACTGACG TGGCTGAGCT GGGTGACGTG GTTAATGGGG TAAAAAAGCT GGTGGAGCTC 
CTGGGCTGGA AGCAGGAATT AGAAGACTTA ATGAATGTTG GCAGAGACAA GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT3 ( XM_009303487.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301762.1 Danio rerio sirtuin 3 (sirt3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42910
Clone ID Related Accession (Same CDS sequence)	XM_009303487.3 , XM_009303487.2
Accession Version	XM_009303487.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTACT TAAATACCTT TTTGCCAAGT GTTTGCAGAC GCTGTTTTGC AGAGAATCTC 
TTATGGAGAA GAGGATTGAC TACTACACAG AATCTGTCAA GAACAAAGCT CGTTCATCAA 
AAGACTCTGT CACATTTCCC TCATGCACAG AAGGGTGCTG CATTTCTTTC ACAGTTCATT 
TATTGCCCAG CTGCCTTCAT TAAATGTGGT GGAACAAGAG GCCTGTTTGG AGGAGGCCGT 
GACAATGTTC ACCAGCAAAC CCTTGAAGAC ATTGCAGAAA AAATCAGAGA ACGCAAGTTT 
AAACGAATTG TGGTGATGGC AGGGGCAGGC ATCAGCACAC CAAGTGGAAT CCCAGACTTC 
AGATCACCCG GCAGCGGCCT TTATGATAAC CTTCAACAGT ACAATCTACC CTATGCTGAA 
GCCATATTTG AAATAAATTA TTTCCATCAT AATCCTAACC CTTTTTTCGC TCTTGCCAAA 
GAGCTTTATC CTGGTAATTA CCAACCCAAC CTGACGCACT ACTTCATTCG CATGCTTCAC 
GATAAGGAAC AGCTGCTCAG GATGTACACG CAAAACATTG ATGGACTTGA GCGCATGGCT 
GGCATTCCTC CAAAGATGCT TGTGGAGGCA CATGGAACAT TTGCCACTGC CACATGCACA 
GTTTGTCGGA GGGATTACAA AGGAGAGGAA CTTCGGGATG ACATCATGGC AGGAACAGTA 
CCCAAATGCC CAACTTGTAA AGGAATCATC AAGCCTGACA TTGTGTTCTT TGGTGAGGAG 
CTTCCACAGC ACTTCTTCAC CTACCTTACA GACTTCCCAA TAGCAGACCT GCTCATTGTG 
ATGGGAACAT CTCTTGAGGT GGAGCCCTTT GCAAGTTTGG CTGGTGCTGT GCGTGGTTCT 
GTACCAAGGC TTTTGATAAA TCGAGACCTT GTGGGGCCGT TTGCCTCGGG GTCCCAACGT 
CACACTGACG TGGCTGAGCT GGGTGACGTG GTTAATGGGG TAAAAAAGCT GGTGGAGCTC 
CTGGGCTGGA AGCAGGAATT AGAAGACTTA ATGAATGTTG GCAGAGACAA GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301762.1
CDS	91..1167
Translation	MLYLNTFLPSVCRRCFAENLLWRRGLTTTQNLSRTKLVHQKTLSHFPHAQKGAAFLSQFIYCPAAFIKCGGTRGLFGGGRDNVHQQTLEDIAEKIRERKFKRIVVMAGAGISTPSGIPDFRSPGSGLYDNLQQYNLPYAEAIFEINYFHHNPNPFFALAKELYPGNYQPNLTHYFIRMLHDKEQLLRMYTQNIDGLERMAGIPPKMLVEAHGTFATATCTVCRRDYKGEELRDDIMAGTVPKCPTCKGIIKPDIVFFGEELPQHFFTYLTDFPIADLLIVMGTSLEVEPFASLAGAVRGSVPRLLINRDLVGPFASGSQRHTDVAELGDVVNGVKKLVELLGWKQELEDLMNVGRDKV

Target ORF information:

RefSeq Version	XM_009303487.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sirtuin 3 (sirt3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303487.2

ORF Insert Sequence:

ATGCTGTACT TAAATACCTT TTTGCCAAGT GTTTGCAGAC GCTGTTTTGC AGAGAATCTC 
TTATGGAGAA GAGGATTGAC TACTACACAG AATCTGTCAA GAACAAAGCT CGTTCATCAA 
AAGACTCTGT CACATTTCCC TCATGCACAG AAGGGTGCTG CATTTCTTTC ACAGTTCATT 
TATTGCCCAG CTGCCTTCAT TAAATGTGGT GGAACAAGAG GCCTGTTTGG AGGAGGCCGT 
GACAATGTTC ACCAGCAAAC CCTTGAAGAC ATTGCAGAAA AAATCAGAGA ACGCAAGTTT 
AAACGAATTG TGGTGATGGC AGGGGCAGGC ATCAGCACAC CAAGTGGAAT CCCAGACTTC 
AGATCACCCG GCAGCGGCCT TTATGATAAC CTTCAACAGT ACAATCTACC CTATGCTGAA 
GCCATATTTG AAATAAATTA TTTCCATCAT AATCCTAACC CTTTTTTCGC TCTTGCCAAA 
GAGCTTTATC CTGGTAATTA CCAACCCAAC CTGACGCACT ACTTCATTCG CATGCTTCAC 
GATAAGGAAC AGCTGCTCAG GATGTACACG CAAAACATTG ATGGACTTGA GCGCATGGCT 
GGCATTCCTC CAAAGATGCT TGTGGAGGCA CATGGAACAT TTGCCACTGC CACATGCACA 
GTTTGTCGGA GGGATTACAA AGGAGAGGAA CTTCGGGATG ACATCATGGC AGGAACAGTA 
CCCAAATGCC CAACTTGTAA AGGAATCATC AAGCCTGACA TTGTGTTCTT TGGTGAGGAG 
CTTCCACAGC ACTTCTTCAC CTACCTTACA GACTTCCCAA TAGCAGACCT GCTCATTGTG 
ATGGGAACAT CTCTTGAGGT GGAGCCCTTT GCAAGTTTGG CTGGTGCTGT GCGTGGTTCT 
GTACCAAGGC TTTTGATAAA TCGAGACCTT GTGGGGCCGT TTGCCTCGGG GTCCCAACGT 
CACACTGACG TGGCTGAGCT GGGTGACGTG GTTAATGGGG TAAAAAAGCT GGTGGAGCTC 
CTGGGCTGGA AGCAGGAATT AGAAGACTTA ATGAATGTTG GCAGAGACAA GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT3 ( XM_009303488.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301763.1 Danio rerio sirtuin 3 (sirt3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42911
Clone ID Related Accession (Same CDS sequence)	XM_009303488.3 , XM_009303488.2
Accession Version	XM_009303488.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303488.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CAGGCATCAG CACACCAAGT GGAATCCCAG ACTTCAGATC ACCCGGCAGC 
GGCCTTTATG ATAACCTTCA ACAGTACAAT CTACCCTATG CTGAAGCCAT ATTTGAAATA 
AATTATTTCC ATCATAATCC TAACCCTTTT TTCGCTCTTG CCAAAGAGCT TTATCCTGGT 
AATTACCAAC CCAACCTGAC GCACTACTTC ATTCGCATGC TTCACGATAA GGAACAGCTG 
CTCAGGATGT ACACGCAAAA CATTGATGGA CTTGAGCGCA TGGCTGGCAT TCCTCCAAAG 
ATGCTTGTGG AGGCACATGG AACATTTGCC ACTGCCACAT GCACAGTTTG TCGGAGGGAT 
TACAAAGGAG AGGAACTTCG GGATGACATC ATGGCAGGAA CAGTACCCAA ATGCCCAACT 
TGTAAAGGAA TCATCAAGCC TGACATTGTG TTCTTTGGTG AGGAGCTTCC ACAGCACTTC 
TTCACCTACC TTACAGACTT CCCAATAGCA GACCTGCTCA TTGTGATGGG AACATCTCTT 
GAGGTGGAGC CCTTTGCAAG TTTGGCTGGT GCTGTGCGTG GTTCTGTACC AAGGCTTTTG 
ATAAATCGAG ACCTTGTGGG GCCGTTTGCC TCGGGGTCCC AACGTCACAC TGACGTGGCT 
GAGCTGGGTG ACGTGGTTAA TGGGGTAAAA AAGCTGGTGG AGCTCCTGGG CTGGAAGCAG 
GAATTAGAAG ACTTAATGAA TGTTGGCAGA GACAAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301763.1
CDS	451..1212
Translation	MAGAGISTPSGIPDFRSPGSGLYDNLQQYNLPYAEAIFEINYFHHNPNPFFALAKELYPGNYQPNLTHYFIRMLHDKEQLLRMYTQNIDGLERMAGIPPKMLVEAHGTFATATCTVCRRDYKGEELRDDIMAGTVPKCPTCKGIIKPDIVFFGEELPQHFFTYLTDFPIADLLIVMGTSLEVEPFASLAGAVRGSVPRLLINRDLVGPFASGSQRHTDVAELGDVVNGVKKLVELLGWKQELEDLMNVGRDKV

Target ORF information:

RefSeq Version	XM_009303488.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sirtuin 3 (sirt3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303488.3

ORF Insert Sequence:

ATGGCAGGGG CAGGCATCAG CACACCAAGT GGAATCCCAG ACTTCAGATC ACCCGGCAGC 
GGCCTTTATG ATAACCTTCA ACAGTACAAT CTACCCTATG CTGAAGCCAT ATTTGAAATA 
AATTATTTCC ATCATAATCC TAACCCTTTT TTCGCTCTTG CCAAAGAGCT TTATCCTGGT 
AATTACCAAC CCAACCTGAC GCACTACTTC ATTCGCATGC TTCACGATAA GGAACAGCTG 
CTCAGGATGT ACACGCAAAA CATTGATGGA CTTGAGCGCA TGGCTGGCAT TCCTCCAAAG 
ATGCTTGTGG AGGCACATGG AACATTTGCC ACTGCCACAT GCACAGTTTG TCGGAGGGAT 
TACAAAGGAG AGGAACTTCG GGATGACATC ATGGCAGGAA CAGTACCCAA ATGCCCAACT 
TGTAAAGGAA TCATCAAGCC TGACATTGTG TTCTTTGGTG AGGAGCTTCC ACAGCACTTC 
TTCACCTACC TTACAGACTT CCCAATAGCA GACCTGCTCA TTGTGATGGG AACATCTCTT 
GAGGTGGAGC CCTTTGCAAG TTTGGCTGGT GCTGTGCGTG GTTCTGTACC AAGGCTTTTG 
ATAAATCGAG ACCTTGTGGG GCCGTTTGCC TCGGGGTCCC AACGTCACAC TGACGTGGCT 
GAGCTGGGTG ACGTGGTTAA TGGGGTAAAA AAGCTGGTGG AGCTCCTGGG CTGGAAGCAG 
GAATTAGAAG ACTTAATGAA TGTTGGCAGA GACAAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT3 ( XM_009303488.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301763.1 Danio rerio sirtuin 3 (sirt3), transcript variant X2, mRNA.

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CloneID	ODa42911
Clone ID Related Accession (Same CDS sequence)	XM_009303488.3 , XM_009303488.2
Accession Version	XM_009303488.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303488.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CAGGCATCAG CACACCAAGT GGAATCCCAG ACTTCAGATC ACCCGGCAGC 
GGCCTTTATG ATAACCTTCA ACAGTACAAT CTACCCTATG CTGAAGCCAT ATTTGAAATA 
AATTATTTCC ATCATAATCC TAACCCTTTT TTCGCTCTTG CCAAAGAGCT TTATCCTGGT 
AATTACCAAC CCAACCTGAC GCACTACTTC ATTCGCATGC TTCACGATAA GGAACAGCTG 
CTCAGGATGT ACACGCAAAA CATTGATGGA CTTGAGCGCA TGGCTGGCAT TCCTCCAAAG 
ATGCTTGTGG AGGCACATGG AACATTTGCC ACTGCCACAT GCACAGTTTG TCGGAGGGAT 
TACAAAGGAG AGGAACTTCG GGATGACATC ATGGCAGGAA CAGTACCCAA ATGCCCAACT 
TGTAAAGGAA TCATCAAGCC TGACATTGTG TTCTTTGGTG AGGAGCTTCC ACAGCACTTC 
TTCACCTACC TTACAGACTT CCCAATAGCA GACCTGCTCA TTGTGATGGG AACATCTCTT 
GAGGTGGAGC CCTTTGCAAG TTTGGCTGGT GCTGTGCGTG GTTCTGTACC AAGGCTTTTG 
ATAAATCGAG ACCTTGTGGG GCCGTTTGCC TCGGGGTCCC AACGTCACAC TGACGTGGCT 
GAGCTGGGTG ACGTGGTTAA TGGGGTAAAA AAGCTGGTGG AGCTCCTGGG CTGGAAGCAG 
GAATTAGAAG ACTTAATGAA TGTTGGCAGA GACAAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301763.1
CDS	443..1204
Translation	MAGAGISTPSGIPDFRSPGSGLYDNLQQYNLPYAEAIFEINYFHHNPNPFFALAKELYPGNYQPNLTHYFIRMLHDKEQLLRMYTQNIDGLERMAGIPPKMLVEAHGTFATATCTVCRRDYKGEELRDDIMAGTVPKCPTCKGIIKPDIVFFGEELPQHFFTYLTDFPIADLLIVMGTSLEVEPFASLAGAVRGSVPRLLINRDLVGPFASGSQRHTDVAELGDVVNGVKKLVELLGWKQELEDLMNVGRDKV

Target ORF information:

RefSeq Version	XM_009303488.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sirtuin 3 (sirt3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303488.2

ORF Insert Sequence:

ATGGCAGGGG CAGGCATCAG CACACCAAGT GGAATCCCAG ACTTCAGATC ACCCGGCAGC 
GGCCTTTATG ATAACCTTCA ACAGTACAAT CTACCCTATG CTGAAGCCAT ATTTGAAATA 
AATTATTTCC ATCATAATCC TAACCCTTTT TTCGCTCTTG CCAAAGAGCT TTATCCTGGT 
AATTACCAAC CCAACCTGAC GCACTACTTC ATTCGCATGC TTCACGATAA GGAACAGCTG 
CTCAGGATGT ACACGCAAAA CATTGATGGA CTTGAGCGCA TGGCTGGCAT TCCTCCAAAG 
ATGCTTGTGG AGGCACATGG AACATTTGCC ACTGCCACAT GCACAGTTTG TCGGAGGGAT 
TACAAAGGAG AGGAACTTCG GGATGACATC ATGGCAGGAA CAGTACCCAA ATGCCCAACT 
TGTAAAGGAA TCATCAAGCC TGACATTGTG TTCTTTGGTG AGGAGCTTCC ACAGCACTTC 
TTCACCTACC TTACAGACTT CCCAATAGCA GACCTGCTCA TTGTGATGGG AACATCTCTT 
GAGGTGGAGC CCTTTGCAAG TTTGGCTGGT GCTGTGCGTG GTTCTGTACC AAGGCTTTTG 
ATAAATCGAG ACCTTGTGGG GCCGTTTGCC TCGGGGTCCC AACGTCACAC TGACGTGGCT 
GAGCTGGGTG ACGTGGTTAA TGGGGTAAAA AAGCTGGTGG AGCTCCTGGG CTGGAAGCAG 
GAATTAGAAG ACTTAATGAA TGTTGGCAGA GACAAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT3 ( XM_017356877.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212366.1 Danio rerio sirtuin 3 (sirt3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa59637
Clone ID Related Accession (Same CDS sequence)	XM_017356877.2 , XM_017356877.1
Accession Version	XM_017356877.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356877.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTACT TAAATACCTT TTTGCCAAGT GTTTGCAGAC GCTGTTTTGC AGAGAATCTC 
TTATGGAGAA GAGGATTGAC TACTACACAG AATCTGTCAA GAACAAAGCT CGTTCATCAA 
AAGACTCTGT CACATTTCCC TCATGCACAG AAGGGTGCTG CATTTCTTTC ACAGTTCATT 
TATTGCCCAG CTGCCTTCAT TAAATGTGGT GGAACAAGAG GCCTGTTTGG AGGAGGCCGT 
GACAATGTTC ACCAGCAAAC CCTTGAAGAC ATTGCAGAAA AAATCAGAGA ACGCAAGTTT 
AAACGAATTG TGGTGATGGC AGGGGCAGGC ATCAGCACAC CAAGTGGAAT CCCAGACTTC 
AGATCACCCG GCAGCGGCCT TTATGATAAC CTTCAACAGT ACAATCTACC CTATGCTGAA 
GCCATATTTG AAATAAATTA TTTCCATCAT AATCCTAACC CTTTTTTCGC TCTTGCCAAA 
GAGCTTTATC CTGGTAATTA CCAACCCAAC CTGACGCACT ACTTCATTCG CATGCTTCAC 
GATAAGGAAC AGCTGCTCAG GATGTACACG CAAAACATTG ATGGACTTGA GCGCAGAGCC 
ACGTGCACAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212366.1
CDS	102..716
Translation	MLYLNTFLPSVCRRCFAENLLWRRGLTTTQNLSRTKLVHQKTLSHFPHAQKGAAFLSQFIYCPAAFIKCGGTRGLFGGGRDNVHQQTLEDIAEKIRERKFKRIVVMAGAGISTPSGIPDFRSPGSGLYDNLQQYNLPYAEAIFEINYFHHNPNPFFALAKELYPGNYQPNLTHYFIRMLHDKEQLLRMYTQNIDGLERRATCTH

Target ORF information:

RefSeq Version	XM_017356877.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sirtuin 3 (sirt3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356877.2

ORF Insert Sequence:

ATGCTGTACT TAAATACCTT TTTGCCAAGT GTTTGCAGAC GCTGTTTTGC AGAGAATCTC 
TTATGGAGAA GAGGATTGAC TACTACACAG AATCTGTCAA GAACAAAGCT CGTTCATCAA 
AAGACTCTGT CACATTTCCC TCATGCACAG AAGGGTGCTG CATTTCTTTC ACAGTTCATT 
TATTGCCCAG CTGCCTTCAT TAAATGTGGT GGAACAAGAG GCCTGTTTGG AGGAGGCCGT 
GACAATGTTC ACCAGCAAAC CCTTGAAGAC ATTGCAGAAA AAATCAGAGA ACGCAAGTTT 
AAACGAATTG TGGTGATGGC AGGGGCAGGC ATCAGCACAC CAAGTGGAAT CCCAGACTTC 
AGATCACCCG GCAGCGGCCT TTATGATAAC CTTCAACAGT ACAATCTACC CTATGCTGAA 
GCCATATTTG AAATAAATTA TTTCCATCAT AATCCTAACC CTTTTTTCGC TCTTGCCAAA 
GAGCTTTATC CTGGTAATTA CCAACCCAAC CTGACGCACT ACTTCATTCG CATGCTTCAC 
GATAAGGAAC AGCTGCTCAG GATGTACACG CAAAACATTG ATGGACTTGA GCGCAGAGCC 
ACGTGCACAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT3 ( XM_017356877.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212366.1 Danio rerio sirtuin 3 (sirt3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa59637
Clone ID Related Accession (Same CDS sequence)	XM_017356877.2 , XM_017356877.1
Accession Version	XM_017356877.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTACT TAAATACCTT TTTGCCAAGT GTTTGCAGAC GCTGTTTTGC AGAGAATCTC 
TTATGGAGAA GAGGATTGAC TACTACACAG AATCTGTCAA GAACAAAGCT CGTTCATCAA 
AAGACTCTGT CACATTTCCC TCATGCACAG AAGGGTGCTG CATTTCTTTC ACAGTTCATT 
TATTGCCCAG CTGCCTTCAT TAAATGTGGT GGAACAAGAG GCCTGTTTGG AGGAGGCCGT 
GACAATGTTC ACCAGCAAAC CCTTGAAGAC ATTGCAGAAA AAATCAGAGA ACGCAAGTTT 
AAACGAATTG TGGTGATGGC AGGGGCAGGC ATCAGCACAC CAAGTGGAAT CCCAGACTTC 
AGATCACCCG GCAGCGGCCT TTATGATAAC CTTCAACAGT ACAATCTACC CTATGCTGAA 
GCCATATTTG AAATAAATTA TTTCCATCAT AATCCTAACC CTTTTTTCGC TCTTGCCAAA 
GAGCTTTATC CTGGTAATTA CCAACCCAAC CTGACGCACT ACTTCATTCG CATGCTTCAC 
GATAAGGAAC AGCTGCTCAG GATGTACACG CAAAACATTG ATGGACTTGA GCGCAGAGCC 
ACGTGCACAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212366.1
CDS	91..705
Translation	MLYLNTFLPSVCRRCFAENLLWRRGLTTTQNLSRTKLVHQKTLSHFPHAQKGAAFLSQFIYCPAAFIKCGGTRGLFGGGRDNVHQQTLEDIAEKIRERKFKRIVVMAGAGISTPSGIPDFRSPGSGLYDNLQQYNLPYAEAIFEINYFHHNPNPFFALAKELYPGNYQPNLTHYFIRMLHDKEQLLRMYTQNIDGLERRATCTH

Target ORF information:

RefSeq Version	XM_017356877.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sirtuin 3 (sirt3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356877.1

ORF Insert Sequence:

ATGCTGTACT TAAATACCTT TTTGCCAAGT GTTTGCAGAC GCTGTTTTGC AGAGAATCTC 
TTATGGAGAA GAGGATTGAC TACTACACAG AATCTGTCAA GAACAAAGCT CGTTCATCAA 
AAGACTCTGT CACATTTCCC TCATGCACAG AAGGGTGCTG CATTTCTTTC ACAGTTCATT 
TATTGCCCAG CTGCCTTCAT TAAATGTGGT GGAACAAGAG GCCTGTTTGG AGGAGGCCGT 
GACAATGTTC ACCAGCAAAC CCTTGAAGAC ATTGCAGAAA AAATCAGAGA ACGCAAGTTT 
AAACGAATTG TGGTGATGGC AGGGGCAGGC ATCAGCACAC CAAGTGGAAT CCCAGACTTC 
AGATCACCCG GCAGCGGCCT TTATGATAAC CTTCAACAGT ACAATCTACC CTATGCTGAA 
GCCATATTTG AAATAAATTA TTTCCATCAT AATCCTAACC CTTTTTTCGC TCTTGCCAAA 
GAGCTTTATC CTGGTAATTA CCAACCCAAC CTGACGCACT ACTTCATTCG CATGCTTCAC 
GATAAGGAAC AGCTGCTCAG GATGTACACG CAAAACATTG ATGGACTTGA GCGCAGAGCC 
ACGTGCACAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Altered Adipogenesis in Zebrafish Larvae Following High Fat Diet and Chemical Exposure Is Visualised by Stimulated Raman Scattering Microscopy.
International journal of molecular sciences18(4)(2017 Apr)
den Broeder MJ,Moester MJB,Kamstra JH,Cenijn PH,Davidoiu V,Kamminga LM,Ariese F,de Boer JF,Legler J

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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SIRT3 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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