SIRT3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SIRT3
Entrez Gene ID	614027
Full Name	sirtuin 3
General protein information	Preferred Names sirtuin 3 Names NAD-dependent protein deacetylase sirtuin-3, mitochondrial
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005200933.4	XP_005200990.1	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X1
XM_010800731.2	XP_010799033.1	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X2
XM_010800731.3	XP_010799033.1	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X2
XM_005200933.3	XP_005200990.1	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X1
NM_001206669.1	NP_001193598.1	NAD-dependent protein deacetylase sirtuin-3, mitochondrial
XM_024999123.1	XP_024854891.1	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SIRT3	XP_003312891.1
Mus musculus (house mouse)	Sirt3	NP_001171275.1
Xenopus tropicalis (tropical clawed frog)	sirt3	XP_002937698.2
Bos taurus (cattle)	SIRT3	NP_001193598.1
Gallus gallus (chicken)	SIRT3	NP_001186422.1
Danio rerio (zebrafish)	sirt3	NP_001073643.1
Homo sapiens (human)	SIRT3	NP_036371.1
	SIRT3	XP_001116486.2
Canis lupus familiaris (dog)	SIRT3	XP_855809.1
Rattus norvegicus (Norway rat)	Sirt3	NP_001099783.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic variants in SIRT3 transcriptional regulatory region affect promoter activity and fat deposition in three cattle breeds.
Gui L, Hong J, Raza SH, Zan L
Molecular and cellular probes3240-45(2017 Apr)

Haplotype distribution in the class I sirtuin genes and their associations with ultrasound carcass traits in Qinchuan cattle (Bos taurus).
Gui L, Hao R, Zhang Y, Zhao X, Zan L
Molecular and cellular probes29(3)167-71(2015 Jun)

Bovine sirtuins: initial characterization and expression of sirtuins 1 and 3 in liver, muscle, and adipose tissue.
Ghinis-Hozumi Y, Gonz?lez-Gallardo A, Gonz?lez-D?valos L, Antaramian A, Villarroya F, Shimada A, Varela-Echavarr?a A, Mora O
Journal of animal science89(8)2529-36(2011 Aug)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results indicate that the polymorphisms in transcription factor binding sites of SIRT3 promoter may affect the transcriptional activity of SIRT3, and thus alter intramuscular fat content in beef cattle.
Title: Genetic variants in SIRT3 transcriptional regulatory region affect promoter activity and fat deposition in three cattle breeds.

The results indicate that the variations in the class I sirtuin genes and their corresponding genotypes may be considered as molecular markers for economic traits in cattle breeding.
Title: Haplotype distribution in the class I sirtuin genes and their associations with ultrasound carcass traits in Qinchuan cattle (Bos taurus).

The expression profile of sirtuin 1 and sirtuin 3 in the liver, muscle, and adipose tissue of calves and bulls is reported.
Title: Bovine sirtuins: initial characterization and expression of sirtuins 1 and 3 in liver, muscle, and adipose tissue.

The following SIRT3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIRT3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa39996	NM_001206669.1 Latest version!	Bos taurus sirtuin 3 (SIRT3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa15311	XM_005200933.4 Latest version!	Bos taurus sirtuin 3 (SIRT3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa15312	XM_024999123.1 Latest version!	Bos taurus sirtuin 3 (SIRT3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa15312	XM_010800731.3 Latest version!	Bos taurus sirtuin 3 (SIRT3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
OBa15312	XM_010800731.2	Bos taurus sirtuin 3 (SIRT3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa15311	XM_005200933.3	Bos taurus sirtuin 3 (SIRT3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIRT3 ( NM_001206669.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193598.1 Bos taurus sirtuin 3 (SIRT3), mRNA.

Price & Availability↑

CloneID	OBa39996
Clone ID Related Accession (Same CDS sequence)	NM_001206669.1
Accession Version	NM_001206669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-01
Organism	Bos taurus(cattle)
Product	NAD-dependent protein deacetylase sirtuin-3, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EV693057.1 and BC149729.1. On or before May 4, 2011 this sequence version replaced XM_002683729.1, XM_873980.3, XM_002703542.1. ##Evidence-Data-START## Transcript exon combination :: BC149729.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTTGT TTCCTCGTTC AGCTGTTCCA GCCCTCAGGT TCTGGGGCCT GCGAGGTGCG 
CGGGCGTGGA CCCGCTGGCC TCGTATTCCA GGTGGAGGAT GGTCCATATC TTGTTTTGCT 
GGTGCCTCAA GTGACACTGG AGGGGGAGAC CACAGTCAGA AGAAGTTTCT CCTGCAGGAC 
ATCGCTGAGC TGATTAAGAC CAGAGCCTGC CAGAAGGTGG TGGTCATGGT GGGGGCCGGC 
ATCAGCACAC CCAGCGGCAT CCCAGACTTC AGGTCTCCGG GGGTTGGCTA CTACAGCATC 
CTCCAGCAGT ACAAGCTCCC CTACCCTGAG GCCATTTTTG AGCTCTCCTT TTTCTTTCAT 
GACCCCAAGC CATTTTTCAC TTTTGCCAAG AAGCTGTACC CTGGGAACTA TAGGCCCAAT 
GCTACTCACT ACTTCCTCCG ATTGCTACAC GAGAAGGGGC TGCTTCTGCG GCTCTACACC 
CAGAACATCG ACGGGCTCGA GAGAGCATCT GGCATCCCTG ACTCAAAGCT CGTTGAAGCT 
CATGGATCCC TTGCCTCTGC CACCTGCACC GTCTGCCGAA GACCCTACCC AGGGGAGGAC 
TTCTGGGCCG ACGTGATGGC GGACAGGGTC CCCCGCTGCC CCGTCTGCTC TGGCGTCACG 
AAGCCTGACA TCGTGTTCTT CGGGGAGCCG CTGCCTGCTA GGTTCCTGCT GCATCTGGCT 
GACTTCCCCA TGGCAGACCT GCTGCTCATC CTTGGGACCT CCCTGGAGGT GGAACCTTTT 
GCCAGCTTGT CCGATGCTGT GCGGAGCTCG GTGCCCCGAC TGCTCATCAA CCGGGACTTG 
GTGGGGTCCT TGGCTAGGAA TCCTCGGGGC AGGGACGTGG CCCAGCTGGG GGATGTGGTC 
CATGGTGTGA AAAGGCTGGT GGAGCTTCTC GGCTGGACAG ACGACATCCA GGACCTCATC 
CAGAGGGAAA CTGGAAAGTT TGATGGCTGG GACAGACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193598.1
CDS	50..1051
Translation	MALFPRSAVPALRFWGLRGARAWTRWPRIPGGGWSISCFAGASSDTGGGDHSQKKFLLQDIAELIKTRACQKVVVMVGAGISTPSGIPDFRSPGVGYYSILQQYKLPYPEAIFELSFFFHDPKPFFTFAKKLYPGNYRPNATHYFLRLLHEKGLLLRLYTQNIDGLERASGIPDSKLVEAHGSLASATCTVCRRPYPGEDFWADVMADRVPRCPVCSGVTKPDIVFFGEPLPARFLLHLADFPMADLLLILGTSLEVEPFASLSDAVRSSVPRLLINRDLVGSLARNPRGRDVAQLGDVVHGVKRLVELLGWTDDIQDLIQRETGKFDGWDRL

Target ORF information:

RefSeq Version	NM_001206669.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sirtuin 3 (SIRT3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206669.1

ORF Insert Sequence:

ATGGCGTTGT TTCCTCGTTC AGCTGTTCCA GCCCTCAGGT TCTGGGGCCT GCGAGGTGCG 
CGGGCGTGGA CCCGCTGGCC TCGTATTCCA GGTGGAGGAT GGTCCATATC TTGTTTTGCT 
GGTGCCTCAA GTGACACTGG AGGGGGAGAC CACAGTCAGA AGAAGTTTCT CCTGCAGGAC 
ATCGCTGAGC TGATTAAGAC CAGAGCCTGC CAGAAGGTGG TGGTCATGGT GGGGGCCGGC 
ATCAGCACAC CCAGCGGCAT CCCAGACTTC AGGTCTCCGG GGGTTGGCTA CTACAGCATC 
CTCCAGCAGT ACAAGCTCCC CTACCCTGAG GCCATTTTTG AGCTCTCCTT TTTCTTTCAT 
GACCCCAAGC CATTTTTCAC TTTTGCCAAG AAGCTGTACC CTGGGAACTA TAGGCCCAAT 
GCTACTCACT ACTTCCTCCG ATTGCTACAC GAGAAGGGGC TGCTTCTGCG GCTCTACACC 
CAGAACATCG ACGGGCTCGA GAGAGCATCT GGCATCCCTG ACTCAAAGCT CGTTGAAGCT 
CATGGATCCC TTGCCTCTGC CACCTGCACC GTCTGCCGAA GACCCTACCC AGGGGAGGAC 
TTCTGGGCCG ACGTGATGGC GGACAGGGTC CCCCGCTGCC CCGTCTGCTC TGGCGTCACG 
AAGCCTGACA TCGTGTTCTT CGGGGAGCCG CTGCCTGCTA GGTTCCTGCT GCATCTGGCT 
GACTTCCCCA TGGCAGACCT GCTGCTCATC CTTGGGACCT CCCTGGAGGT GGAACCTTTT 
GCCAGCTTGT CCGATGCTGT GCGGAGCTCG GTGCCCCGAC TGCTCATCAA CCGGGACTTG 
GTGGGGTCCT TGGCTAGGAA TCCTCGGGGC AGGGACGTGG CCCAGCTGGG GGATGTGGTC 
CATGGTGTGA AAAGGCTGGT GGAGCTTCTC GGCTGGACAG ACGACATCCA GGACCTCATC 
CAGAGGGAAA CTGGAAAGTT TGATGGCTGG GACAGACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT3 ( XM_005200933.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005200990.1 Bos taurus sirtuin 3 (SIRT3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa15311
Clone ID Related Accession (Same CDS sequence)	XM_005200933.4 , XM_005200933.3
Accession Version	XM_005200933.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005200933.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTGT TTCCTCGTTC AGCTGTTCCA GCCCTCAGGT TCTGGGGCCT GCGAGGTGCG 
CGGGCGTGGA CCCGCTGGCC TCGTATTCCA GGTGGAGGAT GGTCCATATC TTGTTTTGCT 
GGTGCCTCAA GTGACACTGG AGGGGGAGAC CACAGTCAGA AGAAGTTTCT CCTGCAGGAC 
ATCGCTGAGC TGATTAAGAC CAGAGCCTGC CAGAAGGTGG TGGTCATGGT GGGGGCCGGC 
ATCAGCACAC CCAGCGGCAT CCCAGACTTC AGGTCTCCGG GGGTTGGCTA CTACAGCATC 
CTCCAGCAGT ACAAGCTCCC CTACCCTGAG GCCATTTTTG AGCTCTCCTT TTTCTTTCAT 
GACCCCAAGC CATTTTTCAC TTTTGCCAAG AAGCTGTACC CTGGGAACTA TAGGCCCAAT 
GCTACTCACT ACTTCCTCCG ATTGCTACAC GAGAAGGGGC TGCTTCTGCG GCTCTACACC 
CAGAACATCG ACGGGCTCGA GAGAGCATCT GGCATCCCTG ACTCAAAGCT CGTTGAAGCT 
CATGGATCCC TTGCCTCTGC CACCTGCACC GTCTGCCGAA GACCCTACCC AGGGGAGGAC 
TTCTGGGTGG AACCTTTTGC CAGCTTGTCC GATGCTGTGC GGAGCTCGGT GCCCCGACTG 
CTCATCAACC GGGACTTGGT GGGGTCCTTG GCTAGGAATC CTCGGGGCAG GGACGTGGCC 
CAGCTGGGGG ATGTGGTCCA TGGTGTGAAA AGGCTGGTGG AGCTTCTCGG CTGGACAGAC 
GACATCCAGG ACCTCATCCA GAGGGAAACT GGAAAGTTTG ATGGCTGGGA CAGACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005200990.1
CDS	63..902
Translation	MALFPRSAVPALRFWGLRGARAWTRWPRIPGGGWSISCFAGASSDTGGGDHSQKKFLLQDIAELIKTRACQKVVVMVGAGISTPSGIPDFRSPGVGYYSILQQYKLPYPEAIFELSFFFHDPKPFFTFAKKLYPGNYRPNATHYFLRLLHEKGLLLRLYTQNIDGLERASGIPDSKLVEAHGSLASATCTVCRRPYPGEDFWVEPFASLSDAVRSSVPRLLINRDLVGSLARNPRGRDVAQLGDVVHGVKRLVELLGWTDDIQDLIQRETGKFDGWDRL

Target ORF information:

RefSeq Version	XM_005200933.4
Organism	Bos taurus(cattle)
Definition	Bos taurus sirtuin 3 (SIRT3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005200933.4

ORF Insert Sequence:

ATGGCGTTGT TTCCTCGTTC AGCTGTTCCA GCCCTCAGGT TCTGGGGCCT GCGAGGTGCG 
CGGGCGTGGA CCCGCTGGCC TCGTATTCCA GGTGGAGGAT GGTCCATATC TTGTTTTGCT 
GGTGCCTCAA GTGACACTGG AGGGGGAGAC CACAGTCAGA AGAAGTTTCT CCTGCAGGAC 
ATCGCTGAGC TGATTAAGAC CAGAGCCTGC CAGAAGGTGG TGGTCATGGT GGGGGCCGGC 
ATCAGCACAC CCAGCGGCAT CCCAGACTTC AGGTCTCCGG GGGTTGGCTA CTACAGCATC 
CTCCAGCAGT ACAAGCTCCC CTACCCTGAG GCCATTTTTG AGCTCTCCTT TTTCTTTCAT 
GACCCCAAGC CATTTTTCAC TTTTGCCAAG AAGCTGTACC CTGGGAACTA TAGGCCCAAT 
GCTACTCACT ACTTCCTCCG ATTGCTACAC GAGAAGGGGC TGCTTCTGCG GCTCTACACC 
CAGAACATCG ACGGGCTCGA GAGAGCATCT GGCATCCCTG ACTCAAAGCT CGTTGAAGCT 
CATGGATCCC TTGCCTCTGC CACCTGCACC GTCTGCCGAA GACCCTACCC AGGGGAGGAC 
TTCTGGGTGG AACCTTTTGC CAGCTTGTCC GATGCTGTGC GGAGCTCGGT GCCCCGACTG 
CTCATCAACC GGGACTTGGT GGGGTCCTTG GCTAGGAATC CTCGGGGCAG GGACGTGGCC 
CAGCTGGGGG ATGTGGTCCA TGGTGTGAAA AGGCTGGTGG AGCTTCTCGG CTGGACAGAC 
GACATCCAGG ACCTCATCCA GAGGGAAACT GGAAAGTTTG ATGGCTGGGA CAGACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT3 ( XM_005200933.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005200990.1 Bos taurus sirtuin 3 (SIRT3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa15311
Clone ID Related Accession (Same CDS sequence)	XM_005200933.4 , XM_005200933.3
Accession Version	XM_005200933.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003072299.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005200933.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTGT TTCCTCGTTC AGCTGTTCCA GCCCTCAGGT TCTGGGGCCT GCGAGGTGCG 
CGGGCGTGGA CCCGCTGGCC TCGTATTCCA GGTGGAGGAT GGTCCATATC TTGTTTTGCT 
GGTGCCTCAA GTGACACTGG AGGGGGAGAC CACAGTCAGA AGAAGTTTCT CCTGCAGGAC 
ATCGCTGAGC TGATTAAGAC CAGAGCCTGC CAGAAGGTGG TGGTCATGGT GGGGGCCGGC 
ATCAGCACAC CCAGCGGCAT CCCAGACTTC AGGTCTCCGG GGGTTGGCTA CTACAGCATC 
CTCCAGCAGT ACAAGCTCCC CTACCCTGAG GCCATTTTTG AGCTCTCCTT TTTCTTTCAT 
GACCCCAAGC CATTTTTCAC TTTTGCCAAG AAGCTGTACC CTGGGAACTA TAGGCCCAAT 
GCTACTCACT ACTTCCTCCG ATTGCTACAC GAGAAGGGGC TGCTTCTGCG GCTCTACACC 
CAGAACATCG ACGGGCTCGA GAGAGCATCT GGCATCCCTG ACTCAAAGCT CGTTGAAGCT 
CATGGATCCC TTGCCTCTGC CACCTGCACC GTCTGCCGAA GACCCTACCC AGGGGAGGAC 
TTCTGGGTGG AACCTTTTGC CAGCTTGTCC GATGCTGTGC GGAGCTCGGT GCCCCGACTG 
CTCATCAACC GGGACTTGGT GGGGTCCTTG GCTAGGAATC CTCGGGGCAG GGACGTGGCC 
CAGCTGGGGG ATGTGGTCCA TGGTGTGAAA AGGCTGGTGG AGCTTCTCGG CTGGACAGAC 
GACATCCAGG ACCTCATCCA GAGGGAAACT GGAAAGTTTG ATGGCTGGGA CAGACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005200990.1
CDS	56..895
Translation	MALFPRSAVPALRFWGLRGARAWTRWPRIPGGGWSISCFAGASSDTGGGDHSQKKFLLQDIAELIKTRACQKVVVMVGAGISTPSGIPDFRSPGVGYYSILQQYKLPYPEAIFELSFFFHDPKPFFTFAKKLYPGNYRPNATHYFLRLLHEKGLLLRLYTQNIDGLERASGIPDSKLVEAHGSLASATCTVCRRPYPGEDFWVEPFASLSDAVRSSVPRLLINRDLVGSLARNPRGRDVAQLGDVVHGVKRLVELLGWTDDIQDLIQRETGKFDGWDRL

Target ORF information:

RefSeq Version	XM_005200933.3
Organism	Bos taurus(cattle)
Definition	Bos taurus sirtuin 3 (SIRT3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005200933.3

ORF Insert Sequence:

ATGGCGTTGT TTCCTCGTTC AGCTGTTCCA GCCCTCAGGT TCTGGGGCCT GCGAGGTGCG 
CGGGCGTGGA CCCGCTGGCC TCGTATTCCA GGTGGAGGAT GGTCCATATC TTGTTTTGCT 
GGTGCCTCAA GTGACACTGG AGGGGGAGAC CACAGTCAGA AGAAGTTTCT CCTGCAGGAC 
ATCGCTGAGC TGATTAAGAC CAGAGCCTGC CAGAAGGTGG TGGTCATGGT GGGGGCCGGC 
ATCAGCACAC CCAGCGGCAT CCCAGACTTC AGGTCTCCGG GGGTTGGCTA CTACAGCATC 
CTCCAGCAGT ACAAGCTCCC CTACCCTGAG GCCATTTTTG AGCTCTCCTT TTTCTTTCAT 
GACCCCAAGC CATTTTTCAC TTTTGCCAAG AAGCTGTACC CTGGGAACTA TAGGCCCAAT 
GCTACTCACT ACTTCCTCCG ATTGCTACAC GAGAAGGGGC TGCTTCTGCG GCTCTACACC 
CAGAACATCG ACGGGCTCGA GAGAGCATCT GGCATCCCTG ACTCAAAGCT CGTTGAAGCT 
CATGGATCCC TTGCCTCTGC CACCTGCACC GTCTGCCGAA GACCCTACCC AGGGGAGGAC 
TTCTGGGTGG AACCTTTTGC CAGCTTGTCC GATGCTGTGC GGAGCTCGGT GCCCCGACTG 
CTCATCAACC GGGACTTGGT GGGGTCCTTG GCTAGGAATC CTCGGGGCAG GGACGTGGCC 
CAGCTGGGGG ATGTGGTCCA TGGTGTGAAA AGGCTGGTGG AGCTTCTCGG CTGGACAGAC 
GACATCCAGG ACCTCATCCA GAGGGAAACT GGAAAGTTTG ATGGCTGGGA CAGACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT3 ( XM_010800731.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799033.1 Bos taurus sirtuin 3 (SIRT3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa15312
Clone ID Related Accession (Same CDS sequence)	XM_010800731.2 , XM_010800731.3 , XM_024999123.1
Accession Version	XM_010800731.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003072299.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010800731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGGG CCGGCATCAG CACACCCAGC GGCATCCCAG ACTTCAGGTC TCCGGGGGTT 
GGCTACTACA GCATCCTCCA GCAGTACAAG CTCCCCTACC CTGAGGCCAT TTTTGAGCTC 
TCCTTTTTCT TTCATGACCC CAAGCCATTT TTCACTTTTG CCAAGAAGCT GTACCCTGGG 
AACTATAGGC CCAATGCTAC TCACTACTTC CTCCGATTGC TACACGAGAA GGGGCTGCTT 
CTGCGGCTCT ACACCCAGAA CATCGACGGG CTCGAGAGAG CATCTGGCAT CCCTGACTCA 
AAGCTCGTTG AAGCTCATGG ATCCCTTGCC TCTGCCACCT GCACCGTCTG CCGAAGACCC 
TACCCAGGGG AGGACTTCTG GGCCGACGTG ATGGCGGACA GGGTCCCCCG CTGCCCCGTC 
TGCTCTGGCG TCACGAAGCC TGACATCGTG TTCTTCGGGG AGCCGCTGCC TGCTAGGTTC 
CTGCTGCATC TGGCTGACTT CCCCATGGCA GACCTGCTGC TCATCCTTGG GACCTCCCTG 
GAGGTGGAAC CTTTTGCCAG CTTGTCCGAT GCTGTGCGGA GCTCGGTGCC CCGACTGCTC 
ATCAACCGGG ACTTGGTGGG GTCCTTGGCT AGGAATCCTC GGGGCAGGGA CGTGGCCCAG 
CTGGGGGATG TGGTCCATGG TGTGAAAAGG CTGGTGGAGC TTCTCGGCTG GACAGACGAC 
ATCCAGGACC TCATCCAGAG GGAAACTGGA AAGTTTGATG GCTGGGACAG ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799033.1
CDS	239..1015
Translation	MVGAGISTPSGIPDFRSPGVGYYSILQQYKLPYPEAIFELSFFFHDPKPFFTFAKKLYPGNYRPNATHYFLRLLHEKGLLLRLYTQNIDGLERASGIPDSKLVEAHGSLASATCTVCRRPYPGEDFWADVMADRVPRCPVCSGVTKPDIVFFGEPLPARFLLHLADFPMADLLLILGTSLEVEPFASLSDAVRSSVPRLLINRDLVGSLARNPRGRDVAQLGDVVHGVKRLVELLGWTDDIQDLIQRETGKFDGWDRL

Target ORF information:

RefSeq Version	XM_010800731.2
Organism	Bos taurus(cattle)
Definition	Bos taurus sirtuin 3 (SIRT3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800731.2

ORF Insert Sequence:

ATGGTGGGGG CCGGCATCAG CACACCCAGC GGCATCCCAG ACTTCAGGTC TCCGGGGGTT 
GGCTACTACA GCATCCTCCA GCAGTACAAG CTCCCCTACC CTGAGGCCAT TTTTGAGCTC 
TCCTTTTTCT TTCATGACCC CAAGCCATTT TTCACTTTTG CCAAGAAGCT GTACCCTGGG 
AACTATAGGC CCAATGCTAC TCACTACTTC CTCCGATTGC TACACGAGAA GGGGCTGCTT 
CTGCGGCTCT ACACCCAGAA CATCGACGGG CTCGAGAGAG CATCTGGCAT CCCTGACTCA 
AAGCTCGTTG AAGCTCATGG ATCCCTTGCC TCTGCCACCT GCACCGTCTG CCGAAGACCC 
TACCCAGGGG AGGACTTCTG GGCCGACGTG ATGGCGGACA GGGTCCCCCG CTGCCCCGTC 
TGCTCTGGCG TCACGAAGCC TGACATCGTG TTCTTCGGGG AGCCGCTGCC TGCTAGGTTC 
CTGCTGCATC TGGCTGACTT CCCCATGGCA GACCTGCTGC TCATCCTTGG GACCTCCCTG 
GAGGTGGAAC CTTTTGCCAG CTTGTCCGAT GCTGTGCGGA GCTCGGTGCC CCGACTGCTC 
ATCAACCGGG ACTTGGTGGG GTCCTTGGCT AGGAATCCTC GGGGCAGGGA CGTGGCCCAG 
CTGGGGGATG TGGTCCATGG TGTGAAAAGG CTGGTGGAGC TTCTCGGCTG GACAGACGAC 
ATCCAGGACC TCATCCAGAG GGAAACTGGA AAGTTTGATG GCTGGGACAG ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT3 ( XM_010800731.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799033.1 Bos taurus sirtuin 3 (SIRT3), transcript variant X3, mRNA.

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CloneID	OBa15312
Clone ID Related Accession (Same CDS sequence)	XM_010800731.2 , XM_010800731.3 , XM_024999123.1
Accession Version	XM_010800731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010800731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGGG CCGGCATCAG CACACCCAGC GGCATCCCAG ACTTCAGGTC TCCGGGGGTT 
GGCTACTACA GCATCCTCCA GCAGTACAAG CTCCCCTACC CTGAGGCCAT TTTTGAGCTC 
TCCTTTTTCT TTCATGACCC CAAGCCATTT TTCACTTTTG CCAAGAAGCT GTACCCTGGG 
AACTATAGGC CCAATGCTAC TCACTACTTC CTCCGATTGC TACACGAGAA GGGGCTGCTT 
CTGCGGCTCT ACACCCAGAA CATCGACGGG CTCGAGAGAG CATCTGGCAT CCCTGACTCA 
AAGCTCGTTG AAGCTCATGG ATCCCTTGCC TCTGCCACCT GCACCGTCTG CCGAAGACCC 
TACCCAGGGG AGGACTTCTG GGCCGACGTG ATGGCGGACA GGGTCCCCCG CTGCCCCGTC 
TGCTCTGGCG TCACGAAGCC TGACATCGTG TTCTTCGGGG AGCCGCTGCC TGCTAGGTTC 
CTGCTGCATC TGGCTGACTT CCCCATGGCA GACCTGCTGC TCATCCTTGG GACCTCCCTG 
GAGGTGGAAC CTTTTGCCAG CTTGTCCGAT GCTGTGCGGA GCTCGGTGCC CCGACTGCTC 
ATCAACCGGG ACTTGGTGGG GTCCTTGGCT AGGAATCCTC GGGGCAGGGA CGTGGCCCAG 
CTGGGGGATG TGGTCCATGG TGTGAAAAGG CTGGTGGAGC TTCTCGGCTG GACAGACGAC 
ATCCAGGACC TCATCCAGAG GGAAACTGGA AAGTTTGATG GCTGGGACAG ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799033.1
CDS	258..1034
Translation	MVGAGISTPSGIPDFRSPGVGYYSILQQYKLPYPEAIFELSFFFHDPKPFFTFAKKLYPGNYRPNATHYFLRLLHEKGLLLRLYTQNIDGLERASGIPDSKLVEAHGSLASATCTVCRRPYPGEDFWADVMADRVPRCPVCSGVTKPDIVFFGEPLPARFLLHLADFPMADLLLILGTSLEVEPFASLSDAVRSSVPRLLINRDLVGSLARNPRGRDVAQLGDVVHGVKRLVELLGWTDDIQDLIQRETGKFDGWDRL

Target ORF information:

RefSeq Version	XM_010800731.3
Organism	Bos taurus(cattle)
Definition	Bos taurus sirtuin 3 (SIRT3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800731.3

ORF Insert Sequence:

ATGGTGGGGG CCGGCATCAG CACACCCAGC GGCATCCCAG ACTTCAGGTC TCCGGGGGTT 
GGCTACTACA GCATCCTCCA GCAGTACAAG CTCCCCTACC CTGAGGCCAT TTTTGAGCTC 
TCCTTTTTCT TTCATGACCC CAAGCCATTT TTCACTTTTG CCAAGAAGCT GTACCCTGGG 
AACTATAGGC CCAATGCTAC TCACTACTTC CTCCGATTGC TACACGAGAA GGGGCTGCTT 
CTGCGGCTCT ACACCCAGAA CATCGACGGG CTCGAGAGAG CATCTGGCAT CCCTGACTCA 
AAGCTCGTTG AAGCTCATGG ATCCCTTGCC TCTGCCACCT GCACCGTCTG CCGAAGACCC 
TACCCAGGGG AGGACTTCTG GGCCGACGTG ATGGCGGACA GGGTCCCCCG CTGCCCCGTC 
TGCTCTGGCG TCACGAAGCC TGACATCGTG TTCTTCGGGG AGCCGCTGCC TGCTAGGTTC 
CTGCTGCATC TGGCTGACTT CCCCATGGCA GACCTGCTGC TCATCCTTGG GACCTCCCTG 
GAGGTGGAAC CTTTTGCCAG CTTGTCCGAT GCTGTGCGGA GCTCGGTGCC CCGACTGCTC 
ATCAACCGGG ACTTGGTGGG GTCCTTGGCT AGGAATCCTC GGGGCAGGGA CGTGGCCCAG 
CTGGGGGATG TGGTCCATGG TGTGAAAAGG CTGGTGGAGC TTCTCGGCTG GACAGACGAC 
ATCCAGGACC TCATCCAGAG GGAAACTGGA AAGTTTGATG GCTGGGACAG ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT3 ( XM_024999123.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854891.1 Bos taurus sirtuin 3 (SIRT3), transcript variant X2, mRNA.

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CloneID	OBa15312
Clone ID Related Accession (Same CDS sequence)	XM_010800731.2 , XM_010800731.3 , XM_024999123.1
Accession Version	XM_024999123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGGG CCGGCATCAG CACACCCAGC GGCATCCCAG ACTTCAGGTC TCCGGGGGTT 
GGCTACTACA GCATCCTCCA GCAGTACAAG CTCCCCTACC CTGAGGCCAT TTTTGAGCTC 
TCCTTTTTCT TTCATGACCC CAAGCCATTT TTCACTTTTG CCAAGAAGCT GTACCCTGGG 
AACTATAGGC CCAATGCTAC TCACTACTTC CTCCGATTGC TACACGAGAA GGGGCTGCTT 
CTGCGGCTCT ACACCCAGAA CATCGACGGG CTCGAGAGAG CATCTGGCAT CCCTGACTCA 
AAGCTCGTTG AAGCTCATGG ATCCCTTGCC TCTGCCACCT GCACCGTCTG CCGAAGACCC 
TACCCAGGGG AGGACTTCTG GGCCGACGTG ATGGCGGACA GGGTCCCCCG CTGCCCCGTC 
TGCTCTGGCG TCACGAAGCC TGACATCGTG TTCTTCGGGG AGCCGCTGCC TGCTAGGTTC 
CTGCTGCATC TGGCTGACTT CCCCATGGCA GACCTGCTGC TCATCCTTGG GACCTCCCTG 
GAGGTGGAAC CTTTTGCCAG CTTGTCCGAT GCTGTGCGGA GCTCGGTGCC CCGACTGCTC 
ATCAACCGGG ACTTGGTGGG GTCCTTGGCT AGGAATCCTC GGGGCAGGGA CGTGGCCCAG 
CTGGGGGATG TGGTCCATGG TGTGAAAAGG CTGGTGGAGC TTCTCGGCTG GACAGACGAC 
ATCCAGGACC TCATCCAGAG GGAAACTGGA AAGTTTGATG GCTGGGACAG ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854891.1
CDS	395..1171
Translation	MVGAGISTPSGIPDFRSPGVGYYSILQQYKLPYPEAIFELSFFFHDPKPFFTFAKKLYPGNYRPNATHYFLRLLHEKGLLLRLYTQNIDGLERASGIPDSKLVEAHGSLASATCTVCRRPYPGEDFWADVMADRVPRCPVCSGVTKPDIVFFGEPLPARFLLHLADFPMADLLLILGTSLEVEPFASLSDAVRSSVPRLLINRDLVGSLARNPRGRDVAQLGDVVHGVKRLVELLGWTDDIQDLIQRETGKFDGWDRL

Target ORF information:

RefSeq Version	XM_024999123.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sirtuin 3 (SIRT3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999123.1

ORF Insert Sequence:

ATGGTGGGGG CCGGCATCAG CACACCCAGC GGCATCCCAG ACTTCAGGTC TCCGGGGGTT 
GGCTACTACA GCATCCTCCA GCAGTACAAG CTCCCCTACC CTGAGGCCAT TTTTGAGCTC 
TCCTTTTTCT TTCATGACCC CAAGCCATTT TTCACTTTTG CCAAGAAGCT GTACCCTGGG 
AACTATAGGC CCAATGCTAC TCACTACTTC CTCCGATTGC TACACGAGAA GGGGCTGCTT 
CTGCGGCTCT ACACCCAGAA CATCGACGGG CTCGAGAGAG CATCTGGCAT CCCTGACTCA 
AAGCTCGTTG AAGCTCATGG ATCCCTTGCC TCTGCCACCT GCACCGTCTG CCGAAGACCC 
TACCCAGGGG AGGACTTCTG GGCCGACGTG ATGGCGGACA GGGTCCCCCG CTGCCCCGTC 
TGCTCTGGCG TCACGAAGCC TGACATCGTG TTCTTCGGGG AGCCGCTGCC TGCTAGGTTC 
CTGCTGCATC TGGCTGACTT CCCCATGGCA GACCTGCTGC TCATCCTTGG GACCTCCCTG 
GAGGTGGAAC CTTTTGCCAG CTTGTCCGAT GCTGTGCGGA GCTCGGTGCC CCGACTGCTC 
ATCAACCGGG ACTTGGTGGG GTCCTTGGCT AGGAATCCTC GGGGCAGGGA CGTGGCCCAG 
CTGGGGGATG TGGTCCATGG TGTGAAAAGG CTGGTGGAGC TTCTCGGCTG GACAGACGAC 
ATCCAGGACC TCATCCAGAG GGAAACTGGA AAGTTTGATG GCTGGGACAG ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic variants in SIRT3 transcriptional regulatory region affect promoter activity and fat deposition in three cattle breeds.
Molecular and cellular probes3240-45(2017 Apr)
Gui L,Hong J,Raza SH,Zan L

Haplotype distribution in the class I sirtuin genes and their associations with ultrasound carcass traits in Qinchuan cattle (Bos taurus).
Molecular and cellular probes29(3)167-71(2015 Jun)
Gui L,Hao R,Zhang Y,Zhao X,Zan L

Bovine sirtuins: initial characterization and expression of sirtuins 1 and 3 in liver, muscle, and adipose tissue.
Journal of animal science89(8)2529-36(2011 Aug)
Ghinis-Hozumi Y,Gonz?lez-Gallardo A,Gonz?lez-D?valos L,Antaramian A,Villarroya F,Shimada A,Varela-Echavarr?a A,Mora O

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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SIRT3 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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