clgn cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	clgn
Entrez Gene ID	556495
Full Name	calmegin
Synonyms	canx,fj49d10,wu:fj24b04,wu:fj49d10,zgc:153946
General protein information	Preferred Names calmegin Names calmegin calnexin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160003.3	XP_005160060.1	calmegin isoform X1
XM_005160003.4	XP_005160060.1	calmegin isoform X1
NM_001077541.1	NP_001071009.1	calmegin precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLGN	XP_003310574.1
Danio rerio (zebrafish)	clgn	NP_001071009.1
Rattus norvegicus (Norway rat)	Clgn	NP_001102942.1
Neurospora crassa	NCU09265	XP_964700.2
Homo sapiens (human)	CLGN	NP_004353.1
Bos taurus (cattle)	CLGN	NP_001029377.1
	CLGN	XP_001089882.1
Mus musculus (house mouse)	Clgn	NP_034034.2
Xenopus tropicalis (tropical clawed frog)	clgn	XP_002932081.2
Canis lupus familiaris (dog)	CLGN	XP_533285.3
Gallus gallus (chicken)	CLGN	XP_420413.4
Schizosaccharomyces pombe (fission yeast)	cnx1	NP_593612.1
Magnaporthe oryzae (rice blast fungus)	MGG_01607	XP_003714591.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish.
Yang D, Liu Q, Yang M, Wu H, Wang Q, Xiao J, Zhang Y
BMC genomics13319(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following clgn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the clgn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37069	NM_001077541.1 Latest version!	Danio rerio calmegin (clgn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa05948	XM_005160003.4 Latest version!	Danio rerio calmegin (clgn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa05948	XM_005160003.3	Danio rerio calmegin (clgn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

clgn ( NM_001077541.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001071009.1 Danio rerio calmegin (clgn), mRNA.

Price & Availability↑

CloneID	ODa37069
Clone ID Related Accession (Same CDS sequence)	NM_001077541.1
Accession Version	NM_001077541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	calmegin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC124496.1. On Nov 9, 2006 this sequence version replaced XM_679319.1. ##Evidence-Data-START## Transcript exon combination :: BC124496.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAGTTGC GGTGGAGCTG GACGTGCTTC TTGTTACTCT CCATCTCCAT GGTAACATTG 
GCCCAGGATG AAGACCTGGA GGATGAAGAG GAAGCTCAAG TGGAAGATGG AGATTTGGAC 
ATGGAGGCTG ACAGCTCTGA AAGCGTTGAA GCTGATGCTA ATGTGTCCTT TCAGGTAACG 
TACAAAACTC CAGTTCCAAC AGGAGATGTT TTCTTCACTG AGACATTTGA TGACGGATCT 
TTGGGCAGTT GGCAGGTGTC TAAAACAATG AAGGAGGACG CTGATGAAGA TATTGCAAAA 
TATGATGGTA AATGGGAGGT TGAGCCCCTG AAGGAAAATA CAGTGCCTGG TGATTTAGGT 
TTGGTGCTGA AATCTCGTGC CAAGCATCAT GCAATCTCGG CTCTGCTTAA CAAACCATTT 
GTGTTTAAGG ACAAACCTCT CATCGTCCAG TACGAGGTCA ATTTTCAAGA TGGAATTGAC 
TGTGGCGGTG CCTACATCAA GCTTTTGTCA GACTCTAAAG ACTTAGAGCT GGAACAGTTC 
CATGATCGCA CACCGTACAG CATCATGTTT GGGCCAGACA AATGTGGAGA AGACTACAAA 
CTGCACTTCA TCTTCAGACA TAAAAATCCC CTCAATGGAG ACGTGGAAGA AAAACATGCC 
AAACGACCCG ATGTTGATCT GAAGAAGATC TACACTGATA AAAAGACTCA CCTCTTTACA 
CTTGTTCTGA ATCCTGACAA TAGCTACGAG ACCTTCATTG ACCAGTCCAG TGTTAGCAGG 
GGAAGTCTGT TGACCGATGT CGTTCCACCT GTCAACCCTC CAAAAGAGAT CGATGATCCC 
AAAGACTCCA AACCGGAGGA CTGGGATGAG AGAGCCAAAA TCCCAGACCC AGAAGCTGTT 
AAACCTGAAG AGTGGGATGA AGAAGCCTTA GCTAAGGTTC CTGATCCAGA TGCAGTGAAG 
CCTGATGGTT GGCTGGATGA TGAGCCAGAG TTTGTGTCTG ACCCCACTGC TGAAAAGCCT 
GATGACTGGG ATGAGGAAAT GGATGGAGAA TGGGAAGCTC CTCAGGTTCC AAATCCGGCA 
TGTACATCTG CTCCAGGCTG TGGTGAATGG AAGCCACCCA TGGTCAATAA TCCTAAGTAT 
AAGGGCAAAT GGAAAGCTCC TCTTATAGAC AACCCCAACT ACCAGGGTGT GTGGAGTCCA 
CGTAAGATCC CAAACCCCGA CCACTTTGAG GACCTTCACC CATTTCGGAT GGAGCCATTC 
AGAGCTGTGG GTCTGGAGCT TTGGTCTATG ACATCCGACA TTTACTTTGA CAATTTTATT 
ATCACCACAG AGAAGGAAGT GGCGGACCGC TGGGCTGCAG ACAGCTGGGG ACTCAAAAAA 
CTTGTGGCGA GTGCCAACGA GCCTGGAGTG TTGAGTCAGC TGGCACTGGC TGCCGAAGAG 
AGGCCATGGC TGTGGGTGAT TTACATCCTT ACCATCGGTT TGCCAATTGG ACTGGTTGTG 
CTTTTCTGCT GGCCAAAGAA GTCAGATGAT GACTATGTCT ACAAGAAAAT CGATCAGCCC 
AAAACAGAAT CAGAGGAGGT GGCTGAAGAA GAAGAAGAAG AGGAGGATGA GGAGGAAGGG 
GACGGAAAAG GAAAGGATGA TGATGAAGCT AAAGCAGAGG AAGCTACTGA AGCTACTGCA 
GGGGATGAGA ATGGAGATAT GATGGGGCAG GAAAATGGAG AAGATGGAGG AGAAACAGAG 
GAGGAGGAAG AGGAAGAGGA CGAAGAGGAG GAAGAGGCCA TTAAGCCAAA TGAAACGGCC 
TCAGAGGATG AAATGAAGGA CGCCGACGAG GGACTCGAAA GCAGAGAAGA AGAGCCTAAA 
GCTGTGAGAA AGAGAAGAGT ACGAAAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071009.1
CDS	108..2000
Translation	MKLRWSWTCFLLLSISMVTLAQDEDLEDEEEAQVEDGDLDMEADSSESVEADANVSFQVTYKTPVPTGDVFFTETFDDGSLGSWQVSKTMKEDADEDIAKYDGKWEVEPLKENTVPGDLGLVLKSRAKHHAISALLNKPFVFKDKPLIVQYEVNFQDGIDCGGAYIKLLSDSKDLELEQFHDRTPYSIMFGPDKCGEDYKLHFIFRHKNPLNGDVEEKHAKRPDVDLKKIYTDKKTHLFTLVLNPDNSYETFIDQSSVSRGSLLTDVVPPVNPPKEIDDPKDSKPEDWDERAKIPDPEAVKPEEWDEEALAKVPDPDAVKPDGWLDDEPEFVSDPTAEKPDDWDEEMDGEWEAPQVPNPACTSAPGCGEWKPPMVNNPKYKGKWKAPLIDNPNYQGVWSPRKIPNPDHFEDLHPFRMEPFRAVGLELWSMTSDIYFDNFIITTEKEVADRWAADSWGLKKLVASANEPGVLSQLALAAEERPWLWVIYILTIGLPIGLVVLFCWPKKSDDDYVYKKIDQPKTESEEVAEEEEEEEDEEEGDGKGKDDDEAKAEEATEATAGDENGDMMGQENGEDGGETEEEEEEEDEEEEEAIKPNETASEDEMKDADEGLESREEEPKAVRKRRVRKD

Target ORF information:

RefSeq Version	NM_001077541.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calmegin (clgn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077541.1

ORF Insert Sequence:

ATGAAGTTGC GGTGGAGCTG GACGTGCTTC TTGTTACTCT CCATCTCCAT GGTAACATTG 
GCCCAGGATG AAGACCTGGA GGATGAAGAG GAAGCTCAAG TGGAAGATGG AGATTTGGAC 
ATGGAGGCTG ACAGCTCTGA AAGCGTTGAA GCTGATGCTA ATGTGTCCTT TCAGGTAACG 
TACAAAACTC CAGTTCCAAC AGGAGATGTT TTCTTCACTG AGACATTTGA TGACGGATCT 
TTGGGCAGTT GGCAGGTGTC TAAAACAATG AAGGAGGACG CTGATGAAGA TATTGCAAAA 
TATGATGGTA AATGGGAGGT TGAGCCCCTG AAGGAAAATA CAGTGCCTGG TGATTTAGGT 
TTGGTGCTGA AATCTCGTGC CAAGCATCAT GCAATCTCGG CTCTGCTTAA CAAACCATTT 
GTGTTTAAGG ACAAACCTCT CATCGTCCAG TACGAGGTCA ATTTTCAAGA TGGAATTGAC 
TGTGGCGGTG CCTACATCAA GCTTTTGTCA GACTCTAAAG ACTTAGAGCT GGAACAGTTC 
CATGATCGCA CACCGTACAG CATCATGTTT GGGCCAGACA AATGTGGAGA AGACTACAAA 
CTGCACTTCA TCTTCAGACA TAAAAATCCC CTCAATGGAG ACGTGGAAGA AAAACATGCC 
AAACGACCCG ATGTTGATCT GAAGAAGATC TACACTGATA AAAAGACTCA CCTCTTTACA 
CTTGTTCTGA ATCCTGACAA TAGCTACGAG ACCTTCATTG ACCAGTCCAG TGTTAGCAGG 
GGAAGTCTGT TGACCGATGT CGTTCCACCT GTCAACCCTC CAAAAGAGAT CGATGATCCC 
AAAGACTCCA AACCGGAGGA CTGGGATGAG AGAGCCAAAA TCCCAGACCC AGAAGCTGTT 
AAACCTGAAG AGTGGGATGA AGAAGCCTTA GCTAAGGTTC CTGATCCAGA TGCAGTGAAG 
CCTGATGGTT GGCTGGATGA TGAGCCAGAG TTTGTGTCTG ACCCCACTGC TGAAAAGCCT 
GATGACTGGG ATGAGGAAAT GGATGGAGAA TGGGAAGCTC CTCAGGTTCC AAATCCGGCA 
TGTACATCTG CTCCAGGCTG TGGTGAATGG AAGCCACCCA TGGTCAATAA TCCTAAGTAT 
AAGGGCAAAT GGAAAGCTCC TCTTATAGAC AACCCCAACT ACCAGGGTGT GTGGAGTCCA 
CGTAAGATCC CAAACCCCGA CCACTTTGAG GACCTTCACC CATTTCGGAT GGAGCCATTC 
AGAGCTGTGG GTCTGGAGCT TTGGTCTATG ACATCCGACA TTTACTTTGA CAATTTTATT 
ATCACCACAG AGAAGGAAGT GGCGGACCGC TGGGCTGCAG ACAGCTGGGG ACTCAAAAAA 
CTTGTGGCGA GTGCCAACGA GCCTGGAGTG TTGAGTCAGC TGGCACTGGC TGCCGAAGAG 
AGGCCATGGC TGTGGGTGAT TTACATCCTT ACCATCGGTT TGCCAATTGG ACTGGTTGTG 
CTTTTCTGCT GGCCAAAGAA GTCAGATGAT GACTATGTCT ACAAGAAAAT CGATCAGCCC 
AAAACAGAAT CAGAGGAGGT GGCTGAAGAA GAAGAAGAAG AGGAGGATGA GGAGGAAGGG 
GACGGAAAAG GAAAGGATGA TGATGAAGCT AAAGCAGAGG AAGCTACTGA AGCTACTGCA 
GGGGATGAGA ATGGAGATAT GATGGGGCAG GAAAATGGAG AAGATGGAGG AGAAACAGAG 
GAGGAGGAAG AGGAAGAGGA CGAAGAGGAG GAAGAGGCCA TTAAGCCAAA TGAAACGGCC 
TCAGAGGATG AAATGAAGGA CGCCGACGAG GGACTCGAAA GCAGAGAAGA AGAGCCTAAA 
GCTGTGAGAA AGAGAAGAGT ACGAAAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clgn ( XM_005160003.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160060.1 Danio rerio calmegin (clgn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05948
Clone ID Related Accession (Same CDS sequence)	XM_005160003.3 , XM_005160003.4
Accession Version	XM_005160003.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calmegin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160003.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGC GGTGGAGCTG GACGTGCTTC TTGTTACTCT CCATCTCCAT GGTAACATTG 
GCCCAGGATG AAGACCTGGA GGATGAAGAG GAAGCTCAAG TGGAAGATGG AGATTTGGAC 
ATGGAGGCTG ACAGCTCTGA AAGCGTTGAA GCTGATGCTA ATGTGTCCTT TCAGGTAACG 
TACAAAACTC CAGTTCCAAC AGGAGATGTT TTCTTCACTG AGACATTTGA TGACGGATCT 
TTGGGCAGTT GGCAGGTGTC TAAAACAATG AAGGAGGACG CTGATGAAGA TATTGCAAAA 
TATGATGGTA AATGGGAGGT TGAGCCCCTG AAGGAAAATA CAGTGCCTGG TGATTTAGGT 
TTGGTGCTGA AATCTCGTGC CAAGCATCAT GCAATCTCGG CTCTGCTTAA CAAACCATTT 
GTGTTTAAGG ACAAACCTCT CATCGTCCAG TACGAGGTCA ATTTTCAAGA TGGAATTGAC 
TGTGGCGGTG CCTACATCAA GCTTTTGTCA GACTCTAAAG ACTTAGAGCT GGAACAGTTC 
CATGATCGCA CACCGTACAG CATCATGTTT GGGCCAGACA AATGTGGAGA AGACTACAAA 
CTGCACTTCA TCTTCAGACA TAAAAATCCC CTCAATGGAG ACGTGGAAGA AAAACATGCC 
AAACGACCCG ATGTTGATCT GAAGAAGATC TACACTGATA AAAAGACTCA CCTCTTTACA 
CTTGTTCTGA ATCCTGACAA TAGCTACGAG ACCTTCATTG ACCAGTCCAG TGTTAGCAGG 
GGAAGTCTGT TGACCGATGT CGTTCCACCT GTCAACCCTC CAAAAGAGAT CGATGATCCC 
AAAGACTCCA AACCGGAGGA CTGGGATGAG AGAGCCAAAA TCCCAGACCC AGAAGCTGTT 
AAACCTGAAG AGTGGGATGA AGAAGCCTTA GCTAAGGTTC CTGATCCAGA TGCAGTGAAG 
CCTGATGGTT GGCTGGATGA TGAGCCAGAG TTTGTGTCTG ACCCCACTGC TGAAAAGCCT 
GATGACTGGG ATGAGGAAAT GGATGGAGAA TGGGAAGCTC CTCAGGTTCC AAATCCGGCA 
TGTACATCTG CTCCAGGCTG TGGTGAATGG AAGCCACCCA TGGTCAATAA TCCTAAGTAT 
AAGGGCAAAT GGAAAGCTCC TCTTATAGAC AACCCCAACT ACCAGGGTGT GTGGAGTCCA 
CGTAAGATCC CAAACCCCGA CCACTTTGAG GACCTTCACC CATTTCGGAT GGAGCCATTC 
AGAGCTGTGG GTCTGGAGCT TTGGTCTATG ACATCCGACA TTTACTTTGA CAATTTTATT 
ATCACCACAG AGAAGGAAGT GGCGGACCGC TGGGCTGCAG ACAGCTGGGG ACTCAAAAAA 
CTTGTGGCGA GTGCCAACGA GCCTGGAGTG TTGAGTCAGC TGGCACTGGC TGCCGAAGAG 
AGGCCATGGC TGTGGGTGAT TTACATCCTT ACCATCGGTT TGCCAATTGG ACTGGTTGTG 
CTTTTCTGCT GGCCAAAGAA GTCAGATGAT GACTATGTCT ACAAGAAAAT CGATCAGCCC 
AAAACAGAAT CAGAGGAGGT GGCTGAAGAA GAAGAAGAAG AGGAGGATGA GGAGGAAGGG 
GACGGAAAAG GAAAGGATGA TGATGAAGCT AAAGCAGAGG AAGCTACTGA AGCTACTGGG 
GATGAGAATG GAGATATGAT GGGGCAGGAA AATGGAGAAG ATGGAGGAGA AACAGAGGAG 
GAGGAAGAGG AAGAGGACGA AGAGGAGGAA GAGGCCATTA AGCCAAATGA AACGGCCTCA 
GAGGATGAAA TGAAGGACGC CGACGAGGGA CTCGAAAGCA GAGAAGAAGA GCCTAAAGCT 
GTGAGAAAGA GAAGAGTACG AAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160060.1
CDS	343..2232
Translation	MKLRWSWTCFLLLSISMVTLAQDEDLEDEEEAQVEDGDLDMEADSSESVEADANVSFQVTYKTPVPTGDVFFTETFDDGSLGSWQVSKTMKEDADEDIAKYDGKWEVEPLKENTVPGDLGLVLKSRAKHHAISALLNKPFVFKDKPLIVQYEVNFQDGIDCGGAYIKLLSDSKDLELEQFHDRTPYSIMFGPDKCGEDYKLHFIFRHKNPLNGDVEEKHAKRPDVDLKKIYTDKKTHLFTLVLNPDNSYETFIDQSSVSRGSLLTDVVPPVNPPKEIDDPKDSKPEDWDERAKIPDPEAVKPEEWDEEALAKVPDPDAVKPDGWLDDEPEFVSDPTAEKPDDWDEEMDGEWEAPQVPNPACTSAPGCGEWKPPMVNNPKYKGKWKAPLIDNPNYQGVWSPRKIPNPDHFEDLHPFRMEPFRAVGLELWSMTSDIYFDNFIITTEKEVADRWAADSWGLKKLVASANEPGVLSQLALAAEERPWLWVIYILTIGLPIGLVVLFCWPKKSDDDYVYKKIDQPKTESEEVAEEEEEEEDEEEGDGKGKDDDEAKAEEATEATGDENGDMMGQENGEDGGETEEEEEEEDEEEEEAIKPNETASEDEMKDADEGLESREEEPKAVRKRRVRKD

Target ORF information:

RefSeq Version	XM_005160003.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calmegin (clgn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160003.3

ORF Insert Sequence:

ATGAAGTTGC GGTGGAGCTG GACGTGCTTC TTGTTACTCT CCATCTCCAT GGTAACATTG 
GCCCAGGATG AAGACCTGGA GGATGAAGAG GAAGCTCAAG TGGAAGATGG AGATTTGGAC 
ATGGAGGCTG ACAGCTCTGA AAGCGTTGAA GCTGATGCTA ATGTGTCCTT TCAGGTAACG 
TACAAAACTC CAGTTCCAAC AGGAGATGTT TTCTTCACTG AGACATTTGA TGACGGATCT 
TTGGGCAGTT GGCAGGTGTC TAAAACAATG AAGGAGGACG CTGATGAAGA TATTGCAAAA 
TATGATGGTA AATGGGAGGT TGAGCCCCTG AAGGAAAATA CAGTGCCTGG TGATTTAGGT 
TTGGTGCTGA AATCTCGTGC CAAGCATCAT GCAATCTCGG CTCTGCTTAA CAAACCATTT 
GTGTTTAAGG ACAAACCTCT CATCGTCCAG TACGAGGTCA ATTTTCAAGA TGGAATTGAC 
TGTGGCGGTG CCTACATCAA GCTTTTGTCA GACTCTAAAG ACTTAGAGCT GGAACAGTTC 
CATGATCGCA CACCGTACAG CATCATGTTT GGGCCAGACA AATGTGGAGA AGACTACAAA 
CTGCACTTCA TCTTCAGACA TAAAAATCCC CTCAATGGAG ACGTGGAAGA AAAACATGCC 
AAACGACCCG ATGTTGATCT GAAGAAGATC TACACTGATA AAAAGACTCA CCTCTTTACA 
CTTGTTCTGA ATCCTGACAA TAGCTACGAG ACCTTCATTG ACCAGTCCAG TGTTAGCAGG 
GGAAGTCTGT TGACCGATGT CGTTCCACCT GTCAACCCTC CAAAAGAGAT CGATGATCCC 
AAAGACTCCA AACCGGAGGA CTGGGATGAG AGAGCCAAAA TCCCAGACCC AGAAGCTGTT 
AAACCTGAAG AGTGGGATGA AGAAGCCTTA GCTAAGGTTC CTGATCCAGA TGCAGTGAAG 
CCTGATGGTT GGCTGGATGA TGAGCCAGAG TTTGTGTCTG ACCCCACTGC TGAAAAGCCT 
GATGACTGGG ATGAGGAAAT GGATGGAGAA TGGGAAGCTC CTCAGGTTCC AAATCCGGCA 
TGTACATCTG CTCCAGGCTG TGGTGAATGG AAGCCACCCA TGGTCAATAA TCCTAAGTAT 
AAGGGCAAAT GGAAAGCTCC TCTTATAGAC AACCCCAACT ACCAGGGTGT GTGGAGTCCA 
CGTAAGATCC CAAACCCCGA CCACTTTGAG GACCTTCACC CATTTCGGAT GGAGCCATTC 
AGAGCTGTGG GTCTGGAGCT TTGGTCTATG ACATCCGACA TTTACTTTGA CAATTTTATT 
ATCACCACAG AGAAGGAAGT GGCGGACCGC TGGGCTGCAG ACAGCTGGGG ACTCAAAAAA 
CTTGTGGCGA GTGCCAACGA GCCTGGAGTG TTGAGTCAGC TGGCACTGGC TGCCGAAGAG 
AGGCCATGGC TGTGGGTGAT TTACATCCTT ACCATCGGTT TGCCAATTGG ACTGGTTGTG 
CTTTTCTGCT GGCCAAAGAA GTCAGATGAT GACTATGTCT ACAAGAAAAT CGATCAGCCC 
AAAACAGAAT CAGAGGAGGT GGCTGAAGAA GAAGAAGAAG AGGAGGATGA GGAGGAAGGG 
GACGGAAAAG GAAAGGATGA TGATGAAGCT AAAGCAGAGG AAGCTACTGA AGCTACTGGG 
GATGAGAATG GAGATATGAT GGGGCAGGAA AATGGAGAAG ATGGAGGAGA AACAGAGGAG 
GAGGAAGAGG AAGAGGACGA AGAGGAGGAA GAGGCCATTA AGCCAAATGA AACGGCCTCA 
GAGGATGAAA TGAAGGACGC CGACGAGGGA CTCGAAAGCA GAGAAGAAGA GCCTAAAGCT 
GTGAGAAAGA GAAGAGTACG AAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clgn ( XM_005160003.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160060.1 Danio rerio calmegin (clgn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05948
Clone ID Related Accession (Same CDS sequence)	XM_005160003.3 , XM_005160003.4
Accession Version	XM_005160003.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calmegin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160003.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGC GGTGGAGCTG GACGTGCTTC TTGTTACTCT CCATCTCCAT GGTAACATTG 
GCCCAGGATG AAGACCTGGA GGATGAAGAG GAAGCTCAAG TGGAAGATGG AGATTTGGAC 
ATGGAGGCTG ACAGCTCTGA AAGCGTTGAA GCTGATGCTA ATGTGTCCTT TCAGGTAACG 
TACAAAACTC CAGTTCCAAC AGGAGATGTT TTCTTCACTG AGACATTTGA TGACGGATCT 
TTGGGCAGTT GGCAGGTGTC TAAAACAATG AAGGAGGACG CTGATGAAGA TATTGCAAAA 
TATGATGGTA AATGGGAGGT TGAGCCCCTG AAGGAAAATA CAGTGCCTGG TGATTTAGGT 
TTGGTGCTGA AATCTCGTGC CAAGCATCAT GCAATCTCGG CTCTGCTTAA CAAACCATTT 
GTGTTTAAGG ACAAACCTCT CATCGTCCAG TACGAGGTCA ATTTTCAAGA TGGAATTGAC 
TGTGGCGGTG CCTACATCAA GCTTTTGTCA GACTCTAAAG ACTTAGAGCT GGAACAGTTC 
CATGATCGCA CACCGTACAG CATCATGTTT GGGCCAGACA AATGTGGAGA AGACTACAAA 
CTGCACTTCA TCTTCAGACA TAAAAATCCC CTCAATGGAG ACGTGGAAGA AAAACATGCC 
AAACGACCCG ATGTTGATCT GAAGAAGATC TACACTGATA AAAAGACTCA CCTCTTTACA 
CTTGTTCTGA ATCCTGACAA TAGCTACGAG ACCTTCATTG ACCAGTCCAG TGTTAGCAGG 
GGAAGTCTGT TGACCGATGT CGTTCCACCT GTCAACCCTC CAAAAGAGAT CGATGATCCC 
AAAGACTCCA AACCGGAGGA CTGGGATGAG AGAGCCAAAA TCCCAGACCC AGAAGCTGTT 
AAACCTGAAG AGTGGGATGA AGAAGCCTTA GCTAAGGTTC CTGATCCAGA TGCAGTGAAG 
CCTGATGGTT GGCTGGATGA TGAGCCAGAG TTTGTGTCTG ACCCCACTGC TGAAAAGCCT 
GATGACTGGG ATGAGGAAAT GGATGGAGAA TGGGAAGCTC CTCAGGTTCC AAATCCGGCA 
TGTACATCTG CTCCAGGCTG TGGTGAATGG AAGCCACCCA TGGTCAATAA TCCTAAGTAT 
AAGGGCAAAT GGAAAGCTCC TCTTATAGAC AACCCCAACT ACCAGGGTGT GTGGAGTCCA 
CGTAAGATCC CAAACCCCGA CCACTTTGAG GACCTTCACC CATTTCGGAT GGAGCCATTC 
AGAGCTGTGG GTCTGGAGCT TTGGTCTATG ACATCCGACA TTTACTTTGA CAATTTTATT 
ATCACCACAG AGAAGGAAGT GGCGGACCGC TGGGCTGCAG ACAGCTGGGG ACTCAAAAAA 
CTTGTGGCGA GTGCCAACGA GCCTGGAGTG TTGAGTCAGC TGGCACTGGC TGCCGAAGAG 
AGGCCATGGC TGTGGGTGAT TTACATCCTT ACCATCGGTT TGCCAATTGG ACTGGTTGTG 
CTTTTCTGCT GGCCAAAGAA GTCAGATGAT GACTATGTCT ACAAGAAAAT CGATCAGCCC 
AAAACAGAAT CAGAGGAGGT GGCTGAAGAA GAAGAAGAAG AGGAGGATGA GGAGGAAGGG 
GACGGAAAAG GAAAGGATGA TGATGAAGCT AAAGCAGAGG AAGCTACTGA AGCTACTGGG 
GATGAGAATG GAGATATGAT GGGGCAGGAA AATGGAGAAG ATGGAGGAGA AACAGAGGAG 
GAGGAAGAGG AAGAGGACGA AGAGGAGGAA GAGGCCATTA AGCCAAATGA AACGGCCTCA 
GAGGATGAAA TGAAGGACGC CGACGAGGGA CTCGAAAGCA GAGAAGAAGA GCCTAAAGCT 
GTGAGAAAGA GAAGAGTACG AAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160060.1
CDS	344..2233
Translation	MKLRWSWTCFLLLSISMVTLAQDEDLEDEEEAQVEDGDLDMEADSSESVEADANVSFQVTYKTPVPTGDVFFTETFDDGSLGSWQVSKTMKEDADEDIAKYDGKWEVEPLKENTVPGDLGLVLKSRAKHHAISALLNKPFVFKDKPLIVQYEVNFQDGIDCGGAYIKLLSDSKDLELEQFHDRTPYSIMFGPDKCGEDYKLHFIFRHKNPLNGDVEEKHAKRPDVDLKKIYTDKKTHLFTLVLNPDNSYETFIDQSSVSRGSLLTDVVPPVNPPKEIDDPKDSKPEDWDERAKIPDPEAVKPEEWDEEALAKVPDPDAVKPDGWLDDEPEFVSDPTAEKPDDWDEEMDGEWEAPQVPNPACTSAPGCGEWKPPMVNNPKYKGKWKAPLIDNPNYQGVWSPRKIPNPDHFEDLHPFRMEPFRAVGLELWSMTSDIYFDNFIITTEKEVADRWAADSWGLKKLVASANEPGVLSQLALAAEERPWLWVIYILTIGLPIGLVVLFCWPKKSDDDYVYKKIDQPKTESEEVAEEEEEEEDEEEGDGKGKDDDEAKAEEATEATGDENGDMMGQENGEDGGETEEEEEEEDEEEEEAIKPNETASEDEMKDADEGLESREEEPKAVRKRRVRKD

Target ORF information:

RefSeq Version	XM_005160003.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calmegin (clgn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160003.4

ORF Insert Sequence:

ATGAAGTTGC GGTGGAGCTG GACGTGCTTC TTGTTACTCT CCATCTCCAT GGTAACATTG 
GCCCAGGATG AAGACCTGGA GGATGAAGAG GAAGCTCAAG TGGAAGATGG AGATTTGGAC 
ATGGAGGCTG ACAGCTCTGA AAGCGTTGAA GCTGATGCTA ATGTGTCCTT TCAGGTAACG 
TACAAAACTC CAGTTCCAAC AGGAGATGTT TTCTTCACTG AGACATTTGA TGACGGATCT 
TTGGGCAGTT GGCAGGTGTC TAAAACAATG AAGGAGGACG CTGATGAAGA TATTGCAAAA 
TATGATGGTA AATGGGAGGT TGAGCCCCTG AAGGAAAATA CAGTGCCTGG TGATTTAGGT 
TTGGTGCTGA AATCTCGTGC CAAGCATCAT GCAATCTCGG CTCTGCTTAA CAAACCATTT 
GTGTTTAAGG ACAAACCTCT CATCGTCCAG TACGAGGTCA ATTTTCAAGA TGGAATTGAC 
TGTGGCGGTG CCTACATCAA GCTTTTGTCA GACTCTAAAG ACTTAGAGCT GGAACAGTTC 
CATGATCGCA CACCGTACAG CATCATGTTT GGGCCAGACA AATGTGGAGA AGACTACAAA 
CTGCACTTCA TCTTCAGACA TAAAAATCCC CTCAATGGAG ACGTGGAAGA AAAACATGCC 
AAACGACCCG ATGTTGATCT GAAGAAGATC TACACTGATA AAAAGACTCA CCTCTTTACA 
CTTGTTCTGA ATCCTGACAA TAGCTACGAG ACCTTCATTG ACCAGTCCAG TGTTAGCAGG 
GGAAGTCTGT TGACCGATGT CGTTCCACCT GTCAACCCTC CAAAAGAGAT CGATGATCCC 
AAAGACTCCA AACCGGAGGA CTGGGATGAG AGAGCCAAAA TCCCAGACCC AGAAGCTGTT 
AAACCTGAAG AGTGGGATGA AGAAGCCTTA GCTAAGGTTC CTGATCCAGA TGCAGTGAAG 
CCTGATGGTT GGCTGGATGA TGAGCCAGAG TTTGTGTCTG ACCCCACTGC TGAAAAGCCT 
GATGACTGGG ATGAGGAAAT GGATGGAGAA TGGGAAGCTC CTCAGGTTCC AAATCCGGCA 
TGTACATCTG CTCCAGGCTG TGGTGAATGG AAGCCACCCA TGGTCAATAA TCCTAAGTAT 
AAGGGCAAAT GGAAAGCTCC TCTTATAGAC AACCCCAACT ACCAGGGTGT GTGGAGTCCA 
CGTAAGATCC CAAACCCCGA CCACTTTGAG GACCTTCACC CATTTCGGAT GGAGCCATTC 
AGAGCTGTGG GTCTGGAGCT TTGGTCTATG ACATCCGACA TTTACTTTGA CAATTTTATT 
ATCACCACAG AGAAGGAAGT GGCGGACCGC TGGGCTGCAG ACAGCTGGGG ACTCAAAAAA 
CTTGTGGCGA GTGCCAACGA GCCTGGAGTG TTGAGTCAGC TGGCACTGGC TGCCGAAGAG 
AGGCCATGGC TGTGGGTGAT TTACATCCTT ACCATCGGTT TGCCAATTGG ACTGGTTGTG 
CTTTTCTGCT GGCCAAAGAA GTCAGATGAT GACTATGTCT ACAAGAAAAT CGATCAGCCC 
AAAACAGAAT CAGAGGAGGT GGCTGAAGAA GAAGAAGAAG AGGAGGATGA GGAGGAAGGG 
GACGGAAAAG GAAAGGATGA TGATGAAGCT AAAGCAGAGG AAGCTACTGA AGCTACTGGG 
GATGAGAATG GAGATATGAT GGGGCAGGAA AATGGAGAAG ATGGAGGAGA AACAGAGGAG 
GAGGAAGAGG AAGAGGACGA AGAGGAGGAA GAGGCCATTA AGCCAAATGA AACGGCCTCA 
GAGGATGAAA TGAAGGACGC CGACGAGGGA CTCGAAAGCA GAGAAGAAGA GCCTAAAGCT 
GTGAGAAAGA GAAGAGTACG AAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish.
BMC genomics13319(2012 Jul)
Yang D,Liu Q,Yang M,Wu H,Wang Q,Xiao J,Zhang Y

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clgn cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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