ORF » Species Summary » Bos taurus » CLGN cDNA ORF clone

CLGN cDNA ORF clone, Bos taurus(cattle)

The following CLGN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLGN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa01423 NM_001034205.2
Latest version!		 Bos taurus calmegin (CLGN), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa01423 XM_024977039.1
Latest version!		 Bos taurus calmegin (CLGN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa01423 XM_024977040.1
Latest version!		 Bos taurus calmegin (CLGN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CLGN ( NM_001034205.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001029377.1 Bos taurus calmegin (CLGN), mRNA.
    Price & Availability↑
    CloneID OBa01423
    Clone ID Related Accession (Same CDS sequence) XM_024977040.1 , NM_001034205.2 , XM_024977039.1
    Accession Version NM_001034205.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-19
    Organism Bos taurus(cattle)
    Product calmegin precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC103401.1. On Aug 30, 2012 this sequence version replaced NM_001034205.1. ##Evidence-Data-START## Transcript exon combination :: BC103401.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCGTTTCC AAGGCTTTTG GCTATGTTTG GGTCTTCTTT TCATCTCAGT TAATGCAGAA 
    TTTATGGATG ATAGTGTTGA GATGGAAGAC TTTGATGAAA ATTCAGAAGA GACTGATGAA 
    CTCTCCTCAG AGATTAAATA TAAGACACCT CAACCTGTAG GAGAAGTGTA TTTTACAGAA 
    ACTTTTGATA GTGGAAGGCT GGCTGGGTGG GTATTATCAA AAGCAAAGAA AGATGATATA 
    GATGCGGAGA TTTCTATATA TGATGGAAGA TGGGAAATAG AAGAATTGAA AGAAAATCGA 
    ATACCTGGTG ATCGAGGACT GGTGTTGAAA TCTAGAGCAA AGCATCATGC AATATCTGCT 
    GTGTTAGCAA AACCCTTCAT CTTTGCTGAT AAACCTCTGG TAGTTCAATA TGAAGTAAAT 
    TTTCAAGATG GTATTGATTG TGGAGGTGCA TACATTAAAC TCCTAGCAGA CACTGATGGT 
    TTGAATCTGG AAAATTTCTA TGATAAAACA TCCTATACCA TTATGTTTGG GCCAGATAAA 
    TGTGGAGAAG ATTATAAACT TCATTTTATC TTCAGACATA AACATCCCAA AACTGGAGTT 
    TTTGAAGAAA AACATGCCAA ACCTCCAGAT GTAGACCTTA AAAGGTTCTT CACAGACAGG 
    AAGACTCATC TTTATACCCT TGTGATGAAT CCAGATGACA CATTTGAAGT ATTAATTGAT 
    CAGATAGTTG TAAACAAAGG AAGCCTACTA GAGGATGTGG TTCCTCCTAT CAATCCTCCC 
    AAAGAAATTG AAGATCCCAC TGATGAAAAA CCTGATGATT GGGATGAAAG AGCAAAAATC 
    CCTGATGCTT CTGCTGTCAA ACCAGAAGAC TGGGATGAAA GTGAACCTCC CCAAATAGTA 
    GATTCAAGTG CTGTTAAACC TGATGGCTGG CTTGATAATG AACCGGAATT TATTCCTGAT 
    CCTAATGCTG AGAAACCCTT TGACTGGAAT GAAGACATGG ATGGAGAATG GGAGGCACCT 
    CATATTTCTA ACCCAGCATG TCGGATTGGG TGTGGTGAAT GGAGCCCTCC CATGATAGAT 
    AACCCGAAAT ACAAAGGAAT ATGGAGACCG CCAATGATAG ATAACCCTAA CTACCAGGGT 
    ATCTGGAGTC CTCGAAAAAT TCCTAATCCA GATTATTTTG AAGACAATCA CCCATTTCTT 
    CTGACTTCTT TCCGTGCTCT TGGTTTAGAG CTTTGGTCCA TGACCTCTGA TATCTACTTT 
    GATAATTTTA TTATCTGTTC AGAAAAGGAA GTAGCAGACC GCTGGGCTGC AGATGGTTGG 
    GGAATGAAGA TACTGATAGA AAATGCTAAT GAGCCTAGTA TATTCAAACA ATTGATGTCA 
    GCTACTGAAC AGCGCCCGTG GCTTTGGTTC ATTTACCTCT TGACAGCAGC ACTTCCAATA 
    GCATTAATTG GTTCATTTTG TTGGCCAAGA AAAGTAAAGA AAAAATATGA AGATGTGGCT 
    TTCGAAAAAC TGGACATATG TAAACCACAA ACAAAGGGAG CACTGGAGCA GGAAGTGAAG 
    GAAGAGAAGG CAGCCCTGGA GAAGCCAGTT GACTTGGAAG AGGAAAAAAA GCAAAGTGAT 
    GGTGAAATTG TTGAAAAAGA AGAGGAAGGT GAACCTGAGG AAAAGAGTGA AGAAGAAATT 
    GAAATTATAG AAGGACAAGA AGAAGGCAAT AAATCCAATA AGTCTGGATC AGAGGATGAG 
    ATGAAAGAGG CAGATGAGAG CACAGGATCT GGAGATGGGC CAATAAAGTC AGTACGCAAA 
    AGAAGAGTAC GGAAGGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001029377.1
    CDS121..1941
    Translation

    Target ORF information:

    RefSeq Version NM_001034205.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calmegin (CLGN), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001034205.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCGTTTCC AAGGCTTTTG GCTATGTTTG GGTCTTCTTT TCATCTCAGT TAATGCAGAA 
    TTTATGGATG ATAGTGTTGA GATGGAAGAC TTTGATGAAA ATTCAGAAGA GACTGATGAA 
    CTCTCCTCAG AGATTAAATA TAAGACACCT CAACCTGTAG GAGAAGTGTA TTTTACAGAA 
    ACTTTTGATA GTGGAAGGCT GGCTGGGTGG GTATTATCAA AAGCAAAGAA AGATGATATA 
    GATGCGGAGA TTTCTATATA TGATGGAAGA TGGGAAATAG AAGAATTGAA AGAAAATCGA 
    ATACCTGGTG ATCGAGGACT GGTGTTGAAA TCTAGAGCAA AGCATCATGC AATATCTGCT 
    GTGTTAGCAA AACCCTTCAT CTTTGCTGAT AAACCTCTGG TAGTTCAATA TGAAGTAAAT 
    TTTCAAGATG GTATTGATTG TGGAGGTGCA TACATTAAAC TCCTAGCAGA CACTGATGGT 
    TTGAATCTGG AAAATTTCTA TGATAAAACA TCCTATACCA TTATGTTTGG GCCAGATAAA 
    TGTGGAGAAG ATTATAAACT TCATTTTATC TTCAGACATA AACATCCCAA AACTGGAGTT 
    TTTGAAGAAA AACATGCCAA ACCTCCAGAT GTAGACCTTA AAAGGTTCTT CACAGACAGG 
    AAGACTCATC TTTATACCCT TGTGATGAAT CCAGATGACA CATTTGAAGT ATTAATTGAT 
    CAGATAGTTG TAAACAAAGG AAGCCTACTA GAGGATGTGG TTCCTCCTAT CAATCCTCCC 
    AAAGAAATTG AAGATCCCAC TGATGAAAAA CCTGATGATT GGGATGAAAG AGCAAAAATC 
    CCTGATGCTT CTGCTGTCAA ACCAGAAGAC TGGGATGAAA GTGAACCTCC CCAAATAGTA 
    GATTCAAGTG CTGTTAAACC TGATGGCTGG CTTGATAATG AACCGGAATT TATTCCTGAT 
    CCTAATGCTG AGAAACCCTT TGACTGGAAT GAAGACATGG ATGGAGAATG GGAGGCACCT 
    CATATTTCTA ACCCAGCATG TCGGATTGGG TGTGGTGAAT GGAGCCCTCC CATGATAGAT 
    AACCCGAAAT ACAAAGGAAT ATGGAGACCG CCAATGATAG ATAACCCTAA CTACCAGGGT 
    ATCTGGAGTC CTCGAAAAAT TCCTAATCCA GATTATTTTG AAGACAATCA CCCATTTCTT 
    CTGACTTCTT TCCGTGCTCT TGGTTTAGAG CTTTGGTCCA TGACCTCTGA TATCTACTTT 
    GATAATTTTA TTATCTGTTC AGAAAAGGAA GTAGCAGACC GCTGGGCTGC AGATGGTTGG 
    GGAATGAAGA TACTGATAGA AAATGCTAAT GAGCCTAGTA TATTCAAACA ATTGATGTCA 
    GCTACTGAAC AGCGCCCGTG GCTTTGGTTC ATTTACCTCT TGACAGCAGC ACTTCCAATA 
    GCATTAATTG GTTCATTTTG TTGGCCAAGA AAAGTAAAGA AAAAATATGA AGATGTGGCT 
    TTCGAAAAAC TGGACATATG TAAACCACAA ACAAAGGGAG CACTGGAGCA GGAAGTGAAG 
    GAAGAGAAGG CAGCCCTGGA GAAGCCAGTT GACTTGGAAG AGGAAAAAAA GCAAAGTGAT 
    GGTGAAATTG TTGAAAAAGA AGAGGAAGGT GAACCTGAGG AAAAGAGTGA AGAAGAAATT 
    GAAATTATAG AAGGACAAGA AGAAGGCAAT AAATCCAATA AGTCTGGATC AGAGGATGAG 
    ATGAAAGAGG CAGATGAGAG CACAGGATCT GGAGATGGGC CAATAAAGTC AGTACGCAAA 
    AGAAGAGTAC GGAAGGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CLGN ( XM_024977040.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832808.1 Bos taurus calmegin (CLGN), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OBa01423
    Clone ID Related Accession (Same CDS sequence) XM_024977040.1 , NM_001034205.2 , XM_024977039.1
    Accession Version XM_024977040.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calmegin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCGTTTCC AAGGCTTTTG GCTATGTTTG GGTCTTCTTT TCATCTCAGT TAATGCAGAA 
    TTTATGGATG ATAGTGTTGA GATGGAAGAC TTTGATGAAA ATTCAGAAGA GACTGATGAA 
    CTCTCCTCAG AGATTAAATA TAAGACACCT CAACCTGTAG GAGAAGTGTA TTTTACAGAA 
    ACTTTTGATA GTGGAAGGCT GGCTGGGTGG GTATTATCAA AAGCAAAGAA AGATGATATA 
    GATGCGGAGA TTTCTATATA TGATGGAAGA TGGGAAATAG AAGAATTGAA AGAAAATCGA 
    ATACCTGGTG ATCGAGGACT GGTGTTGAAA TCTAGAGCAA AGCATCATGC AATATCTGCT 
    GTGTTAGCAA AACCCTTCAT CTTTGCTGAT AAACCTCTGG TAGTTCAATA TGAAGTAAAT 
    TTTCAAGATG GTATTGATTG TGGAGGTGCA TACATTAAAC TCCTAGCAGA CACTGATGGT 
    TTGAATCTGG AAAATTTCTA TGATAAAACA TCCTATACCA TTATGTTTGG GCCAGATAAA 
    TGTGGAGAAG ATTATAAACT TCATTTTATC TTCAGACATA AACATCCCAA AACTGGAGTT 
    TTTGAAGAAA AACATGCCAA ACCTCCAGAT GTAGACCTTA AAAGGTTCTT CACAGACAGG 
    AAGACTCATC TTTATACCCT TGTGATGAAT CCAGATGACA CATTTGAAGT ATTAATTGAT 
    CAGATAGTTG TAAACAAAGG AAGCCTACTA GAGGATGTGG TTCCTCCTAT CAATCCTCCC 
    AAAGAAATTG AAGATCCCAC TGATGAAAAA CCTGATGATT GGGATGAAAG AGCAAAAATC 
    CCTGATGCTT CTGCTGTCAA ACCAGAAGAC TGGGATGAAA GTGAACCTCC CCAAATAGTA 
    GATTCAAGTG CTGTTAAACC TGATGGCTGG CTTGATAATG AACCGGAATT TATTCCTGAT 
    CCTAATGCTG AGAAACCCTT TGACTGGAAT GAAGACATGG ATGGAGAATG GGAGGCACCT 
    CATATTTCTA ACCCAGCATG TCGGATTGGG TGTGGTGAAT GGAGCCCTCC CATGATAGAT 
    AACCCGAAAT ACAAAGGAAT ATGGAGACCG CCAATGATAG ATAACCCTAA CTACCAGGGT 
    ATCTGGAGTC CTCGAAAAAT TCCTAATCCA GATTATTTTG AAGACAATCA CCCATTTCTT 
    CTGACTTCTT TCCGTGCTCT TGGTTTAGAG CTTTGGTCCA TGACCTCTGA TATCTACTTT 
    GATAATTTTA TTATCTGTTC AGAAAAGGAA GTAGCAGACC GCTGGGCTGC AGATGGTTGG 
    GGAATGAAGA TACTGATAGA AAATGCTAAT GAGCCTAGTA TATTCAAACA ATTGATGTCA 
    GCTACTGAAC AGCGCCCGTG GCTTTGGTTC ATTTACCTCT TGACAGCAGC ACTTCCAATA 
    GCATTAATTG GTTCATTTTG TTGGCCAAGA AAAGTAAAGA AAAAATATGA AGATGTGGCT 
    TTCGAAAAAC TGGACATATG TAAACCACAA ACAAAGGGAG CACTGGAGCA GGAAGTGAAG 
    GAAGAGAAGG CAGCCCTGGA GAAGCCAGTT GACTTGGAAG AGGAAAAAAA GCAAAGTGAT 
    GGTGAAATTG TTGAAAAAGA AGAGGAAGGT GAACCTGAGG AAAAGAGTGA AGAAGAAATT 
    GAAATTATAG AAGGACAAGA AGAAGGCAAT AAATCCAATA AGTCTGGATC AGAGGATGAG 
    ATGAAAGAGG CAGATGAGAG CACAGGATCT GGAGATGGGC CAATAAAGTC AGTACGCAAA 
    AGAAGAGTAC GGAAGGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832808.1
    CDS33..1853
    Translation

    Target ORF information:

    RefSeq Version XM_024977040.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calmegin (CLGN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024977040.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCGTTTCC AAGGCTTTTG GCTATGTTTG GGTCTTCTTT TCATCTCAGT TAATGCAGAA 
    TTTATGGATG ATAGTGTTGA GATGGAAGAC TTTGATGAAA ATTCAGAAGA GACTGATGAA 
    CTCTCCTCAG AGATTAAATA TAAGACACCT CAACCTGTAG GAGAAGTGTA TTTTACAGAA 
    ACTTTTGATA GTGGAAGGCT GGCTGGGTGG GTATTATCAA AAGCAAAGAA AGATGATATA 
    GATGCGGAGA TTTCTATATA TGATGGAAGA TGGGAAATAG AAGAATTGAA AGAAAATCGA 
    ATACCTGGTG ATCGAGGACT GGTGTTGAAA TCTAGAGCAA AGCATCATGC AATATCTGCT 
    GTGTTAGCAA AACCCTTCAT CTTTGCTGAT AAACCTCTGG TAGTTCAATA TGAAGTAAAT 
    TTTCAAGATG GTATTGATTG TGGAGGTGCA TACATTAAAC TCCTAGCAGA CACTGATGGT 
    TTGAATCTGG AAAATTTCTA TGATAAAACA TCCTATACCA TTATGTTTGG GCCAGATAAA 
    TGTGGAGAAG ATTATAAACT TCATTTTATC TTCAGACATA AACATCCCAA AACTGGAGTT 
    TTTGAAGAAA AACATGCCAA ACCTCCAGAT GTAGACCTTA AAAGGTTCTT CACAGACAGG 
    AAGACTCATC TTTATACCCT TGTGATGAAT CCAGATGACA CATTTGAAGT ATTAATTGAT 
    CAGATAGTTG TAAACAAAGG AAGCCTACTA GAGGATGTGG TTCCTCCTAT CAATCCTCCC 
    AAAGAAATTG AAGATCCCAC TGATGAAAAA CCTGATGATT GGGATGAAAG AGCAAAAATC 
    CCTGATGCTT CTGCTGTCAA ACCAGAAGAC TGGGATGAAA GTGAACCTCC CCAAATAGTA 
    GATTCAAGTG CTGTTAAACC TGATGGCTGG CTTGATAATG AACCGGAATT TATTCCTGAT 
    CCTAATGCTG AGAAACCCTT TGACTGGAAT GAAGACATGG ATGGAGAATG GGAGGCACCT 
    CATATTTCTA ACCCAGCATG TCGGATTGGG TGTGGTGAAT GGAGCCCTCC CATGATAGAT 
    AACCCGAAAT ACAAAGGAAT ATGGAGACCG CCAATGATAG ATAACCCTAA CTACCAGGGT 
    ATCTGGAGTC CTCGAAAAAT TCCTAATCCA GATTATTTTG AAGACAATCA CCCATTTCTT 
    CTGACTTCTT TCCGTGCTCT TGGTTTAGAG CTTTGGTCCA TGACCTCTGA TATCTACTTT 
    GATAATTTTA TTATCTGTTC AGAAAAGGAA GTAGCAGACC GCTGGGCTGC AGATGGTTGG 
    GGAATGAAGA TACTGATAGA AAATGCTAAT GAGCCTAGTA TATTCAAACA ATTGATGTCA 
    GCTACTGAAC AGCGCCCGTG GCTTTGGTTC ATTTACCTCT TGACAGCAGC ACTTCCAATA 
    GCATTAATTG GTTCATTTTG TTGGCCAAGA AAAGTAAAGA AAAAATATGA AGATGTGGCT 
    TTCGAAAAAC TGGACATATG TAAACCACAA ACAAAGGGAG CACTGGAGCA GGAAGTGAAG 
    GAAGAGAAGG CAGCCCTGGA GAAGCCAGTT GACTTGGAAG AGGAAAAAAA GCAAAGTGAT 
    GGTGAAATTG TTGAAAAAGA AGAGGAAGGT GAACCTGAGG AAAAGAGTGA AGAAGAAATT 
    GAAATTATAG AAGGACAAGA AGAAGGCAAT AAATCCAATA AGTCTGGATC AGAGGATGAG 
    ATGAAAGAGG CAGATGAGAG CACAGGATCT GGAGATGGGC CAATAAAGTC AGTACGCAAA 
    AGAAGAGTAC GGAAGGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CLGN ( XM_024977039.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832807.1 Bos taurus calmegin (CLGN), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBa01423
    Clone ID Related Accession (Same CDS sequence) XM_024977040.1 , NM_001034205.2 , XM_024977039.1
    Accession Version XM_024977039.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calmegin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCGTTTCC AAGGCTTTTG GCTATGTTTG GGTCTTCTTT TCATCTCAGT TAATGCAGAA 
    TTTATGGATG ATAGTGTTGA GATGGAAGAC TTTGATGAAA ATTCAGAAGA GACTGATGAA 
    CTCTCCTCAG AGATTAAATA TAAGACACCT CAACCTGTAG GAGAAGTGTA TTTTACAGAA 
    ACTTTTGATA GTGGAAGGCT GGCTGGGTGG GTATTATCAA AAGCAAAGAA AGATGATATA 
    GATGCGGAGA TTTCTATATA TGATGGAAGA TGGGAAATAG AAGAATTGAA AGAAAATCGA 
    ATACCTGGTG ATCGAGGACT GGTGTTGAAA TCTAGAGCAA AGCATCATGC AATATCTGCT 
    GTGTTAGCAA AACCCTTCAT CTTTGCTGAT AAACCTCTGG TAGTTCAATA TGAAGTAAAT 
    TTTCAAGATG GTATTGATTG TGGAGGTGCA TACATTAAAC TCCTAGCAGA CACTGATGGT 
    TTGAATCTGG AAAATTTCTA TGATAAAACA TCCTATACCA TTATGTTTGG GCCAGATAAA 
    TGTGGAGAAG ATTATAAACT TCATTTTATC TTCAGACATA AACATCCCAA AACTGGAGTT 
    TTTGAAGAAA AACATGCCAA ACCTCCAGAT GTAGACCTTA AAAGGTTCTT CACAGACAGG 
    AAGACTCATC TTTATACCCT TGTGATGAAT CCAGATGACA CATTTGAAGT ATTAATTGAT 
    CAGATAGTTG TAAACAAAGG AAGCCTACTA GAGGATGTGG TTCCTCCTAT CAATCCTCCC 
    AAAGAAATTG AAGATCCCAC TGATGAAAAA CCTGATGATT GGGATGAAAG AGCAAAAATC 
    CCTGATGCTT CTGCTGTCAA ACCAGAAGAC TGGGATGAAA GTGAACCTCC CCAAATAGTA 
    GATTCAAGTG CTGTTAAACC TGATGGCTGG CTTGATAATG AACCGGAATT TATTCCTGAT 
    CCTAATGCTG AGAAACCCTT TGACTGGAAT GAAGACATGG ATGGAGAATG GGAGGCACCT 
    CATATTTCTA ACCCAGCATG TCGGATTGGG TGTGGTGAAT GGAGCCCTCC CATGATAGAT 
    AACCCGAAAT ACAAAGGAAT ATGGAGACCG CCAATGATAG ATAACCCTAA CTACCAGGGT 
    ATCTGGAGTC CTCGAAAAAT TCCTAATCCA GATTATTTTG AAGACAATCA CCCATTTCTT 
    CTGACTTCTT TCCGTGCTCT TGGTTTAGAG CTTTGGTCCA TGACCTCTGA TATCTACTTT 
    GATAATTTTA TTATCTGTTC AGAAAAGGAA GTAGCAGACC GCTGGGCTGC AGATGGTTGG 
    GGAATGAAGA TACTGATAGA AAATGCTAAT GAGCCTAGTA TATTCAAACA ATTGATGTCA 
    GCTACTGAAC AGCGCCCGTG GCTTTGGTTC ATTTACCTCT TGACAGCAGC ACTTCCAATA 
    GCATTAATTG GTTCATTTTG TTGGCCAAGA AAAGTAAAGA AAAAATATGA AGATGTGGCT 
    TTCGAAAAAC TGGACATATG TAAACCACAA ACAAAGGGAG CACTGGAGCA GGAAGTGAAG 
    GAAGAGAAGG CAGCCCTGGA GAAGCCAGTT GACTTGGAAG AGGAAAAAAA GCAAAGTGAT 
    GGTGAAATTG TTGAAAAAGA AGAGGAAGGT GAACCTGAGG AAAAGAGTGA AGAAGAAATT 
    GAAATTATAG AAGGACAAGA AGAAGGCAAT AAATCCAATA AGTCTGGATC AGAGGATGAG 
    ATGAAAGAGG CAGATGAGAG CACAGGATCT GGAGATGGGC CAATAAAGTC AGTACGCAAA 
    AGAAGAGTAC GGAAGGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832807.1
    CDS85..1905
    Translation

    Target ORF information:

    RefSeq Version XM_024977039.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calmegin (CLGN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024977039.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCGTTTCC AAGGCTTTTG GCTATGTTTG GGTCTTCTTT TCATCTCAGT TAATGCAGAA 
    TTTATGGATG ATAGTGTTGA GATGGAAGAC TTTGATGAAA ATTCAGAAGA GACTGATGAA 
    CTCTCCTCAG AGATTAAATA TAAGACACCT CAACCTGTAG GAGAAGTGTA TTTTACAGAA 
    ACTTTTGATA GTGGAAGGCT GGCTGGGTGG GTATTATCAA AAGCAAAGAA AGATGATATA 
    GATGCGGAGA TTTCTATATA TGATGGAAGA TGGGAAATAG AAGAATTGAA AGAAAATCGA 
    ATACCTGGTG ATCGAGGACT GGTGTTGAAA TCTAGAGCAA AGCATCATGC AATATCTGCT 
    GTGTTAGCAA AACCCTTCAT CTTTGCTGAT AAACCTCTGG TAGTTCAATA TGAAGTAAAT 
    TTTCAAGATG GTATTGATTG TGGAGGTGCA TACATTAAAC TCCTAGCAGA CACTGATGGT 
    TTGAATCTGG AAAATTTCTA TGATAAAACA TCCTATACCA TTATGTTTGG GCCAGATAAA 
    TGTGGAGAAG ATTATAAACT TCATTTTATC TTCAGACATA AACATCCCAA AACTGGAGTT 
    TTTGAAGAAA AACATGCCAA ACCTCCAGAT GTAGACCTTA AAAGGTTCTT CACAGACAGG 
    AAGACTCATC TTTATACCCT TGTGATGAAT CCAGATGACA CATTTGAAGT ATTAATTGAT 
    CAGATAGTTG TAAACAAAGG AAGCCTACTA GAGGATGTGG TTCCTCCTAT CAATCCTCCC 
    AAAGAAATTG AAGATCCCAC TGATGAAAAA CCTGATGATT GGGATGAAAG AGCAAAAATC 
    CCTGATGCTT CTGCTGTCAA ACCAGAAGAC TGGGATGAAA GTGAACCTCC CCAAATAGTA 
    GATTCAAGTG CTGTTAAACC TGATGGCTGG CTTGATAATG AACCGGAATT TATTCCTGAT 
    CCTAATGCTG AGAAACCCTT TGACTGGAAT GAAGACATGG ATGGAGAATG GGAGGCACCT 
    CATATTTCTA ACCCAGCATG TCGGATTGGG TGTGGTGAAT GGAGCCCTCC CATGATAGAT 
    AACCCGAAAT ACAAAGGAAT ATGGAGACCG CCAATGATAG ATAACCCTAA CTACCAGGGT 
    ATCTGGAGTC CTCGAAAAAT TCCTAATCCA GATTATTTTG AAGACAATCA CCCATTTCTT 
    CTGACTTCTT TCCGTGCTCT TGGTTTAGAG CTTTGGTCCA TGACCTCTGA TATCTACTTT 
    GATAATTTTA TTATCTGTTC AGAAAAGGAA GTAGCAGACC GCTGGGCTGC AGATGGTTGG 
    GGAATGAAGA TACTGATAGA AAATGCTAAT GAGCCTAGTA TATTCAAACA ATTGATGTCA 
    GCTACTGAAC AGCGCCCGTG GCTTTGGTTC ATTTACCTCT TGACAGCAGC ACTTCCAATA 
    GCATTAATTG GTTCATTTTG TTGGCCAAGA AAAGTAAAGA AAAAATATGA AGATGTGGCT 
    TTCGAAAAAC TGGACATATG TAAACCACAA ACAAAGGGAG CACTGGAGCA GGAAGTGAAG 
    GAAGAGAAGG CAGCCCTGGA GAAGCCAGTT GACTTGGAAG AGGAAAAAAA GCAAAGTGAT 
    GGTGAAATTG TTGAAAAAGA AGAGGAAGGT GAACCTGAGG AAAAGAGTGA AGAAGAAATT 
    GAAATTATAG AAGGACAAGA AGAAGGCAAT AAATCCAATA AGTCTGGATC AGAGGATGAG 
    ATGAAAGAGG CAGATGAGAG CACAGGATCT GGAGATGGGC CAATAAAGTC AGTACGCAAA 
    AGAAGAGTAC GGAAGGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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