hells cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hells
Entrez Gene ID	553328
Full Name	helicase, lymphoid specific
Synonyms	cb65,im:6911667,pasg,sb:cb65,sb:cb749
General protein information	Preferred Names helicase, lymphoid specific Names lymphoid-specific helicase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037101.1	NP_001032178.1	lymphoid-specific helicase

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Hells	NP_001099841.1
Neurospora crassa	NCU06306	XP_962568.2
Rattus norvegicus (Norway rat)	LOC100911660	XP_003749146.2
Gallus gallus (chicken)	HELLS	XP_421626.3
Arabidopsis thaliana (thale cress)	CHR1	NP_201476.1
Danio rerio (zebrafish)	hells	NP_001032178.1
Xenopus tropicalis (tropical clawed frog)	hells	NP_001135693.1

Gene Ontology
Bibliography

Related articles in PubMed

Lsh Is Essential for Maintaining Global DNA Methylation Levels in Amphibia and Fish and Interacts Directly with Dnmt1.
Dunican DS, Pennings S, Meehan RR
BioMed research international2015740637(2015)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Recher G, Jouralet J, Brombin A, Heuz? A, Mugniery E, Hermel JM, Desnoulez S, Savy T, Herbomel P, Bourrat F, Peyri?ras N, Jamen F, Joly JS
Development (Cambridge, England)140(24)4860-9(2013 Dec)

Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.
Baraban SC, Dinday MT, Hortopan GA
Nature communications42410(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lsh Is Essential for Maintaining Global DNA Methylation Levels in Amphibia and Fish and Interacts Directly with Dnmt1.
Title: Lsh Is Essential for Maintaining Global DNA Methylation Levels in Amphibia and Fish and Interacts Directly with Dnmt1.

The following hells gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hells cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00683	NM_001037101.1 Latest version!	Danio rerio helicase, lymphoid specific (hells), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hells ( NM_001037101.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001032178.1 Danio rerio helicase, lymphoid specific (hells), mRNA.

Price & Availability↑

CloneID	ODa00683
Clone ID Related Accession (Same CDS sequence)	NM_001037101.1
Accession Version	NM_001037101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Danio rerio(zebrafish)
Product	lymphoid-specific helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY612850.1. On or before Nov 5, 2005 this sequence version replaced XM_678529.1, XM_704663.1, XM_704662.1, XM_704664.1. ##Evidence-Data-START## Transcript exon combination :: AY612850.1, BC171408.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGGGA TTGTCAAAGA AGAGACTCGT AGTATTTCTC CCCACTGCCC CAAACCTAAC 
GAATCTGAAG AGCCTGAAGG CACTACAATT GAGGAGACCA CAATGGAAAA AAGCAAGCAT 
GAAGAGATGG TCATTACCAA AGAGATGGAG GAAGAAGAAA AGAATCTAGA AGAGGAGGGA 
GAGAAAAAAG AAAGAGAAAT GATGGAGGAG GCCAGACAAT CCAGGGGGAA CGATTCTCAA 
GAAATGCGGT TCAAAAGACT TCAGCACCTT TTGGAGAAAA GCAACATTTA CTCAAAGTTT 
CTTCTTACAA AGATGGAGCA GCAGCAGCAA GAGGAAAAAC TTAAAAAGGA GAGACTGGAA 
AAGAAAGCTG CTGTTCTGAA GCAAAAGGGC AGTGATAAAA AGACTGTAAA AGTTGAGAGA 
AAGAAGCGTG GAAGGGAAGA TTACAAAATT GCAGATGTGA TGACAAAAGA GGAAATTATG 
TCAAAAGCCA AAAAGCCCAA AATGGAAGAG GAGGCTCAAG CATCAGTAAA ACTGGAGGCT 
GAGGATATAG AGAAGCTAAG TGACACAAAC TCTGATATTA AAGGTCGACT CTCTGAGACT 
TTAAGGGAGA ACAGCAAGCA GATGCTGGAT CCAGAAAGAA CGGTGAATGG TCAACCTGTG 
CCAGCACAGC AGCCCCAACT GTTTACTGGG GGTGTGATGA GGTGGTACCA GGTGGAAGGC 
ATAGAGTGGC TCAGGATGCT GTGGGAAAAT GGCATCAACG GCATCCTGGC TGATGAGATG 
GGTCTTGGAA AAACCATTCA GTGCATCGCA CATATTGCAA TGATGGTTGA GAAAAAGGTC 
CTTGGGCCTT TCCTAGTGGT GGCTCCTTTG TCCACTCTTC CAAACTGGAT TTCAGAATTC 
AAGCGTTTCA CTCCAGAGGT GTCTGTCCTG CTGTATCATG GACCTCAAAA AGAGAGGTTG 
GACCTGGTGA AAAAGATCCG TCAGCACCAG GGATCCCTCA GAATGTGCCC TGTTGTTGTT 
ACGTCCTTTG AAATAGCCAT GAGAGACCGG AAGTTTCTTC AGCGTTTCCA CTGGAACTAT 
CTGATTGTGG ATGAGGGTCA CAGGATTAAA AACTTGAACT GTCGTCTGGT GCAAGAACTG 
AAGATGCTGC CGACTGACAA CAAACTGCTC CTGACAGGAA CCCCCCTCCA GAATAACCTG 
TCTGAGCTGT GGTCACTCCT CAATTTCCTC CTTCCTGATG TGTTTGATGA CCTGAAGAGT 
TTTGAATCCT GGTTTGACAT CAGCACCATC ACTTCAGATG CTGAAAATAT TGTGGCTAAC 
GAGCGAGAGC AGAATATTCT TCACATGCTT CATCTGATCC TCACGCCCTT CCTTCTGAGA 
AGATTAAAAT CAGATGTCAC ACTGGAGGTT CCACCTAAGA AAGAGATTGT TGTGTATGCA 
CCCCTAACCA ACAAACAAGA GGCTTTTTAC ATGGCCATTG TTAACAAGAC TATTGCCAAA 
CTGCTGGGTC AGGAGAAGGA TGAATCGGCG CCTGCTCCCC TAACACCCAG TGGCCGACCA 
AAACGCAGGA GCAGGAAGGT GGTGAACTAT ACTGAGCCAA ACACTGACTC TATGAAAGAC 
CTTGAGAAGT ACCTAGAGAA GGTTCAGCAG GAGTTGGACA GTCAGGCAAG CTCTACTCCT 
GTGGTGAATG TGTTTATGCC AGTTGATGCT CAAGTCAACT TGAAACTGCA GAACATCCTG 
ATGCTGCTGA AGAGATGTTG CAATCATGCG TACCTCATAG AGTATCCTCT GGACCCCACA 
ACTGGAGACT TTAAGATTGA TGAACAGTTA GTGGAAGCCT CAGGGAAATT TCTTATTCTG 
GACAGAATGC TTCCTGAACT GAAAAAAAGA GGACACAAGG TGCTGATTTT TAGCCAGATG 
ACCTCCATAT TGGATATTTT GATGGATTAC TGTTATCTCA GGGGTTACGA ATACAGCAGA 
CTGGATGGGT CAATGAGTTA CGCTGACCGT GATGAGAATA TGAAGAAGTT TTCTTCAGAT 
CCAGAGGTGT TTCTTTTTCT CCTGAGCACT CGAGCTGGTG GCCTTGGAAT CAACCTGACA 
TCTGCTGACA CGGTCATCAT ATTTGACAGT GATTGGAATC CTCAGGCAGA TTTGCAGGCA 
CAGGATCGCT GTCACCGTAT TGGGCAAACA AAGCCTGTGG TGGTCCACCG TCTCATTACG 
GCCAACACCA TTGATGAGAA GATTCTGGAG AGAGCTTCTG CCAAGAGAAA GCTAGAAAAG 
ATGGTCATTC ACAAAAACAA GTTTAAAGGC TCTAAAGCAG AACTAAAACA AAGCAAGAGC 
TGCTTGGATG TTGATGAGCT TGTAGAACTA CTGAAGTCCA GAGACTACGA CGGGGCAGTG 
AAAGGCACTA AAGGAAAGGT CATCAGCGAC AAAGATCTGC AGATTTTGCT GGACCGCAGT 
GATTTAATGG ATCAAGCCAA GAAGCGTGTT AAACAGGAGA AAGATGGGGT GTTTAAAGTT 
ATTGAGGCCA AGGATGACAA TGGTGAGATC AGTCTGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032178.1
CDS	200..2761
Translation	MSGIVKEETRSISPHCPKPNESEEPEGTTIEETTMEKSKHEEMVITKEMEEEEKNLEEEGEKKEREMMEEARQSRGNDSQEMRFKRLQHLLEKSNIYSKFLLTKMEQQQQEEKLKKERLEKKAAVLKQKGSDKKTVKVERKKRGREDYKIADVMTKEEIMSKAKKPKMEEEAQASVKLEAEDIEKLSDTNSDIKGRLSETLRENSKQMLDPERTVNGQPVPAQQPQLFTGGVMRWYQVEGIEWLRMLWENGINGILADEMGLGKTIQCIAHIAMMVEKKVLGPFLVVAPLSTLPNWISEFKRFTPEVSVLLYHGPQKERLDLVKKIRQHQGSLRMCPVVVTSFEIAMRDRKFLQRFHWNYLIVDEGHRIKNLNCRLVQELKMLPTDNKLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESWFDISTITSDAENIVANEREQNILHMLHLILTPFLLRRLKSDVTLEVPPKKEIVVYAPLTNKQEAFYMAIVNKTIAKLLGQEKDESAPAPLTPSGRPKRRSRKVVNYTEPNTDSMKDLEKYLEKVQQELDSQASSTPVVNVFMPVDAQVNLKLQNILMLLKRCCNHAYLIEYPLDPTTGDFKIDEQLVEASGKFLILDRMLPELKKRGHKVLIFSQMTSILDILMDYCYLRGYEYSRLDGSMSYADRDENMKKFSSDPEVFLFLLSTRAGGLGINLTSADTVIIFDSDWNPQADLQAQDRCHRIGQTKPVVVHRLITANTIDEKILERASAKRKLEKMVIHKNKFKGSKAELKQSKSCLDVDELVELLKSRDYDGAVKGTKGKVISDKDLQILLDRSDLMDQAKKRVKQEKDGVFKVIEAKDDNGEISLS

Target ORF information:

RefSeq Version	NM_001037101.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio helicase, lymphoid specific (hells), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037101.1

ORF Insert Sequence:

ATGAGTGGGA TTGTCAAAGA AGAGACTCGT AGTATTTCTC CCCACTGCCC CAAACCTAAC 
GAATCTGAAG AGCCTGAAGG CACTACAATT GAGGAGACCA CAATGGAAAA AAGCAAGCAT 
GAAGAGATGG TCATTACCAA AGAGATGGAG GAAGAAGAAA AGAATCTAGA AGAGGAGGGA 
GAGAAAAAAG AAAGAGAAAT GATGGAGGAG GCCAGACAAT CCAGGGGGAA CGATTCTCAA 
GAAATGCGGT TCAAAAGACT TCAGCACCTT TTGGAGAAAA GCAACATTTA CTCAAAGTTT 
CTTCTTACAA AGATGGAGCA GCAGCAGCAA GAGGAAAAAC TTAAAAAGGA GAGACTGGAA 
AAGAAAGCTG CTGTTCTGAA GCAAAAGGGC AGTGATAAAA AGACTGTAAA AGTTGAGAGA 
AAGAAGCGTG GAAGGGAAGA TTACAAAATT GCAGATGTGA TGACAAAAGA GGAAATTATG 
TCAAAAGCCA AAAAGCCCAA AATGGAAGAG GAGGCTCAAG CATCAGTAAA ACTGGAGGCT 
GAGGATATAG AGAAGCTAAG TGACACAAAC TCTGATATTA AAGGTCGACT CTCTGAGACT 
TTAAGGGAGA ACAGCAAGCA GATGCTGGAT CCAGAAAGAA CGGTGAATGG TCAACCTGTG 
CCAGCACAGC AGCCCCAACT GTTTACTGGG GGTGTGATGA GGTGGTACCA GGTGGAAGGC 
ATAGAGTGGC TCAGGATGCT GTGGGAAAAT GGCATCAACG GCATCCTGGC TGATGAGATG 
GGTCTTGGAA AAACCATTCA GTGCATCGCA CATATTGCAA TGATGGTTGA GAAAAAGGTC 
CTTGGGCCTT TCCTAGTGGT GGCTCCTTTG TCCACTCTTC CAAACTGGAT TTCAGAATTC 
AAGCGTTTCA CTCCAGAGGT GTCTGTCCTG CTGTATCATG GACCTCAAAA AGAGAGGTTG 
GACCTGGTGA AAAAGATCCG TCAGCACCAG GGATCCCTCA GAATGTGCCC TGTTGTTGTT 
ACGTCCTTTG AAATAGCCAT GAGAGACCGG AAGTTTCTTC AGCGTTTCCA CTGGAACTAT 
CTGATTGTGG ATGAGGGTCA CAGGATTAAA AACTTGAACT GTCGTCTGGT GCAAGAACTG 
AAGATGCTGC CGACTGACAA CAAACTGCTC CTGACAGGAA CCCCCCTCCA GAATAACCTG 
TCTGAGCTGT GGTCACTCCT CAATTTCCTC CTTCCTGATG TGTTTGATGA CCTGAAGAGT 
TTTGAATCCT GGTTTGACAT CAGCACCATC ACTTCAGATG CTGAAAATAT TGTGGCTAAC 
GAGCGAGAGC AGAATATTCT TCACATGCTT CATCTGATCC TCACGCCCTT CCTTCTGAGA 
AGATTAAAAT CAGATGTCAC ACTGGAGGTT CCACCTAAGA AAGAGATTGT TGTGTATGCA 
CCCCTAACCA ACAAACAAGA GGCTTTTTAC ATGGCCATTG TTAACAAGAC TATTGCCAAA 
CTGCTGGGTC AGGAGAAGGA TGAATCGGCG CCTGCTCCCC TAACACCCAG TGGCCGACCA 
AAACGCAGGA GCAGGAAGGT GGTGAACTAT ACTGAGCCAA ACACTGACTC TATGAAAGAC 
CTTGAGAAGT ACCTAGAGAA GGTTCAGCAG GAGTTGGACA GTCAGGCAAG CTCTACTCCT 
GTGGTGAATG TGTTTATGCC AGTTGATGCT CAAGTCAACT TGAAACTGCA GAACATCCTG 
ATGCTGCTGA AGAGATGTTG CAATCATGCG TACCTCATAG AGTATCCTCT GGACCCCACA 
ACTGGAGACT TTAAGATTGA TGAACAGTTA GTGGAAGCCT CAGGGAAATT TCTTATTCTG 
GACAGAATGC TTCCTGAACT GAAAAAAAGA GGACACAAGG TGCTGATTTT TAGCCAGATG 
ACCTCCATAT TGGATATTTT GATGGATTAC TGTTATCTCA GGGGTTACGA ATACAGCAGA 
CTGGATGGGT CAATGAGTTA CGCTGACCGT GATGAGAATA TGAAGAAGTT TTCTTCAGAT 
CCAGAGGTGT TTCTTTTTCT CCTGAGCACT CGAGCTGGTG GCCTTGGAAT CAACCTGACA 
TCTGCTGACA CGGTCATCAT ATTTGACAGT GATTGGAATC CTCAGGCAGA TTTGCAGGCA 
CAGGATCGCT GTCACCGTAT TGGGCAAACA AAGCCTGTGG TGGTCCACCG TCTCATTACG 
GCCAACACCA TTGATGAGAA GATTCTGGAG AGAGCTTCTG CCAAGAGAAA GCTAGAAAAG 
ATGGTCATTC ACAAAAACAA GTTTAAAGGC TCTAAAGCAG AACTAAAACA AAGCAAGAGC 
TGCTTGGATG TTGATGAGCT TGTAGAACTA CTGAAGTCCA GAGACTACGA CGGGGCAGTG 
AAAGGCACTA AAGGAAAGGT CATCAGCGAC AAAGATCTGC AGATTTTGCT GGACCGCAGT 
GATTTAATGG ATCAAGCCAA GAAGCGTGTT AAACAGGAGA AAGATGGGGT GTTTAAAGTT 
ATTGAGGCCA AGGATGACAA TGGTGAGATC AGTCTGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lsh Is Essential for Maintaining Global DNA Methylation Levels in Amphibia and Fish and Interacts Directly with Dnmt1.
BioMed research international2015740637(2015)
Dunican DS,Pennings S,Meehan RR

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Development (Cambridge, England)140(24)4860-9(2013 Dec)
Recher G,Jouralet J,Brombin A,Heuz? A,Mugniery E,Hermel JM,Desnoulez S,Savy T,Herbomel P,Bourrat F,Peyri?ras N,Jamen F,Joly JS

Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.
Nature communications42410(2013)
Baraban SC,Dinday MT,Hortopan GA

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hells cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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