SMYD2A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMYD2A
Entrez Gene ID	541423
Full Name	SET and MYND domain containing 2a
Synonyms	im:7146196,smyd2,wu:fc02f02,zgc:110553
General protein information	Preferred Names SET and MYND domain containing 2a Names N-lysine methyltransferase SMYD2-A SET and MYND domain containing 2 SET and MYND domain-containing protein 2A histone methyltransferase SMYD2-A
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013550.1	NP_001013568.1	N-lysine methyltransferase SMYD2-A
XM_017351633.1	XP_017207122.1	N-lysine methyltransferase SMYD2-A isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMYD2	XP_537149.3
Xenopus tropicalis (tropical clawed frog)	LOC100495522	XP_002934751.2
Homo sapiens (human)	SMYD2	NP_064582.2
Rattus norvegicus (Norway rat)	Smyd2	NP_996733.1
Danio rerio (zebrafish)	smyd2a	NP_001013568.1
Pan troglodytes (chimpanzee)	SMYD2	XP_003308794.1
	SMYD2	XP_001106482.2
Bos taurus (cattle)	SMYD2	NP_001069832.1
Mus musculus (house mouse)	Smyd2	NP_081072.1
Gallus gallus (chicken)	SMYD2	NP_001264500.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Proper Activity of Histone H3 Lysine 4 (H3K4) Methyltransferase Is Required for Morphogenesis during Zebrafish Cardiogenesis.
Kim JD, Kim E, Koun S, Ham HJ, Rhee M, Kim MJ, Huh TL
Molecules and cells38(6)580-6(2015 Jun)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

SMYD2 is induced during cell differentiation and participates in early development.
Ses? B, Barrero MJ, Fabregat MC, Sander V, Izpisua Belmonte JC
The International journal of developmental biology57(5)357-64(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings suggest that SMYD2 (SET and MYND domain containing protein 2), a histone lysine methyltransferase, plays a critical role at early stages of development.
Title: SMYD2 is induced during cell differentiation and participates in early development.

The following SMYD2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMYD2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00347	NM_001013550.1 Latest version!	Danio rerio SET and MYND domain containing 2a (smyd2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa55577	XM_017351633.1 Latest version!	Danio rerio SET and MYND domain containing 2a (smyd2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMYD2A ( NM_001013550.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001013568.1 Danio rerio SET and MYND domain containing 2a (smyd2a), mRNA.

Price & Availability↑

CloneID	ODa00347
Clone ID Related Accession (Same CDS sequence)	NM_001013550.1
Accession Version	NM_001013550.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Danio rerio(zebrafish)
Product	N-lysine methyltransferase SMYD2-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC091465.1. On Jul 6, 2005 this sequence version replaced XM_696610.1. ##Evidence-Data-START## Transcript exon combination :: BC091465.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAAAAAG AGGGAATCGA GGGTACTGAG AGGTTTCTGA GCCCCGGTAA AGGCCGGGGA 
CTCAAAGCCA TCAAGCATTT TAAAGTTGGT GATCTAGTGT TCGCCTGTCC GGCTTATGCA 
TACGTGCTGA CTGTGAACGA GAGAGGCGGA CGCTGCGAAT GCTGCTTTAC CAGGAAAGAA 
GGGCTGTCGA AGTGTGGGAA ATGTAAACAA GCCTATTACT GCAATGTAGA GTGTCAGAGA 
GGAGACTGGC CCATGCACAA GCTGGAGTGT TCTGCCATGT GTGCGTATGG GGAGAATTGG 
TGTCCATCTG AAACCGTACG ACTGGTCGCC AGAATCATTC TCAAACAGAA GCACCAAACA 
GAAAGGACGC CTTCAGAGAG AGTGCTGACG CTTCGAGAAT TAGAAGCACA TCTGGACAAG 
CTGGATAATG AGAAGAATGA GATGAACGAT ACAGACATTG CTGCGCTACA CCACTTTTAC 
TCTCGACATC TCGATTTTCC TGATAATGCA GCACTTACTG AACTTATCGC TCAGGTGAAC 
TGTAATGGCT TCACCATTGA AGATGAAGAG CTGTCTCATT TGGGATCTGC TTTATTTCCA 
GACGTTGCTC TGATGAATCA CAGCTGTAGC CCAAATGTGA TTGTGACGTA TAAGGGTACA 
GTGGCAGAAG TGAGAGCCGT GCAGGAGATC AACCCTGAAG AGGAGATCTT CAACAGTTAT 
ATTGACCTCC TATATCCAAC AGAAGATAGA ATTGAACGAC TGAAAGACTC TTACTTCTTT 
AATTGTGACT GTAAAGAATG CACCTCAAAA TCAAAGGATG AAGCCAAAAT GGAGATCCGT 
CAGAAGCTCA GCATACCTCC AGAGGAAGAG GAAATCAAAC AGATGGTGAT ATACGCAAGA 
AATGTCATTG AGGAGTTCAG GAGAGCAAAG CACTACAAAA CCCCCAGTGA ATTGTTAGAA 
ATCTGTGAAT TAAGCATGGA GAAAATGGGT GCAATCTTCG CCGAAACCAA CGTGTACATG 
CTGCACATGA TGTATCAGGC AATGGGTGTC TGTCTCTATA TGCAGGATTG GGACGGAGCG 
ATGAAATACG GTGAAAAAAT TATCCATCCC TACAGTGTGC ACTACCCTCC ATACTCCCTG 
AATGTGGCTT CAATGTACCT GAAACTGGGA CGACTGTATC TAGGACTTGA AAAGAGGACT 
CAAGGAGTTA AAGCTTTAAA GAAGGCTCTC GCAATCATGG ACATTGCCCA CGGAAAAGAC 
CACCCTTACA TCGATGAAAT TAAGAAGGAA ATGGAAGAGC AGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013568.1
CDS	144..1451
Translation	MKKEGIEGTERFLSPGKGRGLKAIKHFKVGDLVFACPAYAYVLTVNERGGRCECCFTRKEGLSKCGKCKQAYYCNVECQRGDWPMHKLECSAMCAYGENWCPSETVRLVARIILKQKHQTERTPSERVLTLRELEAHLDKLDNEKNEMNDTDIAALHHFYSRHLDFPDNAALTELIAQVNCNGFTIEDEELSHLGSALFPDVALMNHSCSPNVIVTYKGTVAEVRAVQEINPEEEIFNSYIDLLYPTEDRIERLKDSYFFNCDCKECTSKSKDEAKMEIRQKLSIPPEEEEIKQMVIYARNVIEEFRRAKHYKTPSELLEICELSMEKMGAIFAETNVYMLHMMYQAMGVCLYMQDWDGAMKYGEKIIHPYSVHYPPYSLNVASMYLKLGRLYLGLEKRTQGVKALKKALAIMDIAHGKDHPYIDEIKKEMEEQT

Target ORF information:

RefSeq Version	NM_001013550.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SET and MYND domain containing 2a (smyd2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013550.1

ORF Insert Sequence:

ATGAAAAAAG AGGGAATCGA GGGTACTGAG AGGTTTCTGA GCCCCGGTAA AGGCCGGGGA 
CTCAAAGCCA TCAAGCATTT TAAAGTTGGT GATCTAGTGT TCGCCTGTCC GGCTTATGCA 
TACGTGCTGA CTGTGAACGA GAGAGGCGGA CGCTGCGAAT GCTGCTTTAC CAGGAAAGAA 
GGGCTGTCGA AGTGTGGGAA ATGTAAACAA GCCTATTACT GCAATGTAGA GTGTCAGAGA 
GGAGACTGGC CCATGCACAA GCTGGAGTGT TCTGCCATGT GTGCGTATGG GGAGAATTGG 
TGTCCATCTG AAACCGTACG ACTGGTCGCC AGAATCATTC TCAAACAGAA GCACCAAACA 
GAAAGGACGC CTTCAGAGAG AGTGCTGACG CTTCGAGAAT TAGAAGCACA TCTGGACAAG 
CTGGATAATG AGAAGAATGA GATGAACGAT ACAGACATTG CTGCGCTACA CCACTTTTAC 
TCTCGACATC TCGATTTTCC TGATAATGCA GCACTTACTG AACTTATCGC TCAGGTGAAC 
TGTAATGGCT TCACCATTGA AGATGAAGAG CTGTCTCATT TGGGATCTGC TTTATTTCCA 
GACGTTGCTC TGATGAATCA CAGCTGTAGC CCAAATGTGA TTGTGACGTA TAAGGGTACA 
GTGGCAGAAG TGAGAGCCGT GCAGGAGATC AACCCTGAAG AGGAGATCTT CAACAGTTAT 
ATTGACCTCC TATATCCAAC AGAAGATAGA ATTGAACGAC TGAAAGACTC TTACTTCTTT 
AATTGTGACT GTAAAGAATG CACCTCAAAA TCAAAGGATG AAGCCAAAAT GGAGATCCGT 
CAGAAGCTCA GCATACCTCC AGAGGAAGAG GAAATCAAAC AGATGGTGAT ATACGCAAGA 
AATGTCATTG AGGAGTTCAG GAGAGCAAAG CACTACAAAA CCCCCAGTGA ATTGTTAGAA 
ATCTGTGAAT TAAGCATGGA GAAAATGGGT GCAATCTTCG CCGAAACCAA CGTGTACATG 
CTGCACATGA TGTATCAGGC AATGGGTGTC TGTCTCTATA TGCAGGATTG GGACGGAGCG 
ATGAAATACG GTGAAAAAAT TATCCATCCC TACAGTGTGC ACTACCCTCC ATACTCCCTG 
AATGTGGCTT CAATGTACCT GAAACTGGGA CGACTGTATC TAGGACTTGA AAAGAGGACT 
CAAGGAGTTA AAGCTTTAAA GAAGGCTCTC GCAATCATGG ACATTGCCCA CGGAAAAGAC 
CACCCTTACA TCGATGAAAT TAAGAAGGAA ATGGAAGAGC AGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMYD2A ( XM_017351633.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207122.1 Danio rerio SET and MYND domain containing 2a (smyd2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa55577
Clone ID Related Accession (Same CDS sequence)	XM_017351633.1
Accession Version	XM_017351633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	N-lysine methyltransferase SMYD2-A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAAGC TGGAGTGTTC TGCCATGTGT GCGTATGGGG AGAATTGGTG TCCGTCTGAA 
ACCGTACGAC TGGTCGCCAG AATCATTCTC AAACAGAAGC ACCAAACAGA AAGGACGCCT 
TCAGAGAGAG TGCTGACGCT TCGAGAATTA GAAGCACATC TGGACAAGCT GGATAATGAG 
AAGAATGAGA TGAACGATAC AGACATTGCT GCGCTACACC ACTTTTACTC TCGACATCTC 
GATTTTCCTG ATAATGCAGC ACTTACTGAA CTTATCGCTC AGGTGAACTG TAATGGCTTC 
ACCATTGAAG ATGAAGAGCT GTCTCATTTG GGATCTGCTT TATTTCCAGA CGTTGCTCTG 
ATGAATCACA GCTGTAGCCC AAATGTGATT GTGACGTATA AGGGTACAGT GGCAGAAGTG 
AGAGCCGTGC AGGAGATCAA CCCTGAAGAG GAGATCTTCA ACAGTTATAT TGACCTCCTA 
TATCCAACAG AAGATAGAAT TGAACGACTG AAAGACTCTT ACTTCTTTAA TTGTGACTGT 
AAAGAATGCA CCTCAAAATC AAAGGATGAA GCCAAAATGG AGATCCGTCA GAAGCTCAGC 
ACACCTCCAG AGGAAGAGGA AATCAAACAG ATGGTGATAT ACGCAAGAAA TGTCATTGAG 
GAGTTCAGGA GAGCAAAGCA CTACAAAACC CCCAGTGAAT TGTTGGAAAT CTGTGAATTA 
AGCATGGAGA AAATGGGTGC AATCTTCGCC GAAACCAACG TGTACATGCT GCACATGATG 
TATCAGGCAA TGGGTGTCTG TCTCTATATG CAGGATTGGG ACGGAGCGAT GAAATACGGT 
GAAAAAATTA TCCATCCCTA CAGTGTGCAC TACCCTCCAT ACTCCCTGAA TGTGGCTTCA 
ATGTACCTGA AACTGGGACG ACTGTATCTA GGACTTGAAA AGAGGACTCA AGGAGTTAAA 
GCTTTAAAGA AGGCTCTCGC AATCATGGAC ATTGCCCACG GAAAAGACCA CCCTTACATC 
GATGAAATTA AGAAGGAAAT GGAAGAGCAG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207122.1
CDS	225..1280
Translation	MHKLECSAMCAYGENWCPSETVRLVARIILKQKHQTERTPSERVLTLRELEAHLDKLDNEKNEMNDTDIAALHHFYSRHLDFPDNAALTELIAQVNCNGFTIEDEELSHLGSALFPDVALMNHSCSPNVIVTYKGTVAEVRAVQEINPEEEIFNSYIDLLYPTEDRIERLKDSYFFNCDCKECTSKSKDEAKMEIRQKLSTPPEEEEIKQMVIYARNVIEEFRRAKHYKTPSELLEICELSMEKMGAIFAETNVYMLHMMYQAMGVCLYMQDWDGAMKYGEKIIHPYSVHYPPYSLNVASMYLKLGRLYLGLEKRTQGVKALKKALAIMDIAHGKDHPYIDEIKKEMEEQT

Target ORF information:

RefSeq Version	XM_017351633.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SET and MYND domain containing 2a (smyd2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351633.1

ORF Insert Sequence:

ATGCACAAGC TGGAGTGTTC TGCCATGTGT GCGTATGGGG AGAATTGGTG TCCGTCTGAA 
ACCGTACGAC TGGTCGCCAG AATCATTCTC AAACAGAAGC ACCAAACAGA AAGGACGCCT 
TCAGAGAGAG TGCTGACGCT TCGAGAATTA GAAGCACATC TGGACAAGCT GGATAATGAG 
AAGAATGAGA TGAACGATAC AGACATTGCT GCGCTACACC ACTTTTACTC TCGACATCTC 
GATTTTCCTG ATAATGCAGC ACTTACTGAA CTTATCGCTC AGGTGAACTG TAATGGCTTC 
ACCATTGAAG ATGAAGAGCT GTCTCATTTG GGATCTGCTT TATTTCCAGA CGTTGCTCTG 
ATGAATCACA GCTGTAGCCC AAATGTGATT GTGACGTATA AGGGTACAGT GGCAGAAGTG 
AGAGCCGTGC AGGAGATCAA CCCTGAAGAG GAGATCTTCA ACAGTTATAT TGACCTCCTA 
TATCCAACAG AAGATAGAAT TGAACGACTG AAAGACTCTT ACTTCTTTAA TTGTGACTGT 
AAAGAATGCA CCTCAAAATC AAAGGATGAA GCCAAAATGG AGATCCGTCA GAAGCTCAGC 
ACACCTCCAG AGGAAGAGGA AATCAAACAG ATGGTGATAT ACGCAAGAAA TGTCATTGAG 
GAGTTCAGGA GAGCAAAGCA CTACAAAACC CCCAGTGAAT TGTTGGAAAT CTGTGAATTA 
AGCATGGAGA AAATGGGTGC AATCTTCGCC GAAACCAACG TGTACATGCT GCACATGATG 
TATCAGGCAA TGGGTGTCTG TCTCTATATG CAGGATTGGG ACGGAGCGAT GAAATACGGT 
GAAAAAATTA TCCATCCCTA CAGTGTGCAC TACCCTCCAT ACTCCCTGAA TGTGGCTTCA 
ATGTACCTGA AACTGGGACG ACTGTATCTA GGACTTGAAA AGAGGACTCA AGGAGTTAAA 
GCTTTAAAGA AGGCTCTCGC AATCATGGAC ATTGCCCACG GAAAAGACCA CCCTTACATC 
GATGAAATTA AGAAGGAAAT GGAAGAGCAG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Proper Activity of Histone H3 Lysine 4 (H3K4) Methyltransferase Is Required for Morphogenesis during Zebrafish Cardiogenesis.
Molecules and cells38(6)580-6(2015 Jun)
Kim JD,Kim E,Koun S,Ham HJ,Rhee M,Kim MJ,Huh TL

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

SMYD2 is induced during cell differentiation and participates in early development.
The International journal of developmental biology57(5)357-64(2013)
Ses? B,Barrero MJ,Fabregat MC,Sander V,Izpisua Belmonte JC

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SMYD2A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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