dhtkd1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dhtkd1
Entrez Gene ID	494076
Full Name	dehydrogenase E1 and transketolase domain containing 1
Synonyms	zgc:101818
General protein information	Preferred Names dehydrogenase E1 and transketolase domain containing 1 Names probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial dehydrogenase E1 and transketolase domain-containing protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008619.1	NP_001008619.1	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DHTKD1	XP_001135864.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000551	XP_310532.5
	LOC696445	XP_001085071.2
Mus musculus (house mouse)	Dhtkd1	NP_001074600.1
Bos taurus (cattle)	DHTKD1	NP_001192767.1
Rattus norvegicus (Norway rat)	Dhtkd1	NP_001020891.1
Danio rerio (zebrafish)	dhtkd1	NP_001008619.1
Homo sapiens (human)	DHTKD1	NP_061176.3
Canis lupus familiaris (dog)	DHTKD1	XP_535192.3
Gallus gallus (chicken)	DHTKD1	XP_004937543.1
Drosophila melanogaster (fruit fly)	CG1544	NP_651849.1
Caenorhabditis elegans (roundworm)	ZK836.2	NP_001256410.1
Xenopus tropicalis (tropical clawed frog)	dhtkd1	XP_002931921.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A ?Raf1-ER-inducible oncogenic zebrafish liver cell model identifies hepatocellular carcinoma signatures.
He S, Krens SG, Zhan H, Gong Z, Hogendoorn PC, Spaink HP, Snaar-Jagalska BE
The Journal of pathology225(1)19-28(2011 Sep)

Identification and expression of liver-specific genes after LPS challenge in amphioxus: the hepatic cecum as liver-like organ and "pre-hepatic" acute phase response.
Wang Y, Zhang S
Functional & integrative genomics11(1)111-8(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dhtkd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dhtkd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37520	NM_001008619.1 Latest version!	Danio rerio dehydrogenase E1 and transketolase domain containing 1 (dhtkd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dhtkd1 ( NM_001008619.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001008619.1 Danio rerio dehydrogenase E1 and transketolase domain containing 1 (dhtkd1), mRNA.

Price & Availability↑

CloneID	ODa37520
Clone ID Related Accession (Same CDS sequence)	NM_001008619.1
Accession Version	NM_001008619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC086742.1. ##Evidence-Data-START## Transcript exon combination :: BC086742.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCCGCGC GCAGTATGCT TATACACCTC CCTCGGCTTT TGCACCGCTC CTTTGGTGCC 
AGGAGGTCGT TTTTAGCGAG TTTGTACCAC ACTCAGCGTG GTGTGTACGG TTTCAGACCG 
AGAGACGGTC CAGAAAGACG GGCAGAGTTA ACGCAGATCT CCGCTCTTAA CCGAGATCAC 
GGACTGGCGA GACTGGTGGA GGCCTATCGA GCACATGGAC ACAAAGTAGC TAAAATTAAC 
CCCTTACTAC CACAAAGTCC CGTGCTGGAG AGTGTACCGG AGATAAGCCT GCTGAATGGA 
GCTGTTCGTG GAGTCCTCAA CACTACCGGG TTGCGGCACT TTGGTAAAGC TGAGGCGACT 
GCAGAAGAGG TTGTGGCGTA TCTGGAAAGC ACATACTGTG GGGGAATCTC AGTGGAAACC 
TCTCAGCTGC AGACCTTAGA GGAACGAGAA TGGTTCGCTG ACCGATTTGA GGAGCTCAAA 
AAAGAGGCGT TTTCTCCAGA GGAAAGGAGA ATGCTGGCTA AACTGATGCT CGAGTCACAG 
GAATTTGACC ACTTTTTAGC CACCAAGTTT GCAACTGTGA AGCGGTATGG AGGCGAGGGA 
GCAGAGAGCA TGATGGGATT TTTCTACGAG CTGTTTCGCT CTTCAGTATA CAGTGGAGTG 
ACGGATGTGG TCATGGGAAT GCCTCACCGC GGACGCCTTA ATCTGCTCAC CGGCCTTCTT 
CAGTTTCCTC CTGAGCTCAT GTTTCGTAAG ATGAGGGGTC TCAGTGAGTT TCCGGAGCAC 
TCTCCCTCTA TCGGAGACGT CTTGTCCCAT TTAACGTCTA CAGTGGAGCT TGACTTCGGG 
GCAGGACACC CTCTCCATGT CACAATGCTG CCCAATCCTT CCCACCTGGA GGCCATCAAC 
CCTGTGACAC AGGGGAAAAC ACGAGGCCGA CAGCAGGTCA AACAGGACGG CGACTACTCC 
ACTGACCCTC ATTCACGACC GGGAGACAAA GTCATCTGCC TGCAGGTTCA TGGCGATGCT 
TCATTTTCAG GACAAGGAAT AGTACCGGAA ACCTTCACCT TGTCAAATCT CCCTCATTAT 
AGAGTGGGTG GAAGCATCCA CCTCATTGTA AACAATCAAG TGGGCTACAC CACTCCCTCT 
GAGAGGGGAA GATCATCTCT CTACTGTAGT GATGTTGGTA AAATGGTGGG ATGTGCAATA 
ATCCATGTGA ATGGCGATGA TGCTGAAGAA GTCCTCAGAG CGACCCGGCT GGCGGTGGAG 
TACCAGAGAC GCTTCAGAAA AGACGTCATT GTTGATCTGC TTTGTTACCG GCAGTGGGGC 
CACAATGAGC TGGACGAACC CTTTTTCACA AACCCTGCAA TGTATAAAAT AATACGGTCA 
AGAAAAAGCA TCCCAGATTC CTATGCTGAC CAGCTGATCT CTGAGGGTCT GATGACAGAC 
GAGGAGCGCA GTCAGATCAA GACCAGCTAC TACGCTACGC TCAACAACCA GCTGACTAAC 
ATGACCCTCT ACAGCCCTCC ACCCACAAAC CTGCAGGGCC GCTGGGGAGA CCTGGTGGAG 
CCCCAGAATA GAGTCTCGTG CTGGGACACT GGCGTCGCAC AGCCCCTGCT GCAGTTTGTT 
GGAGCCAAAT CAGTGGACAT ACCTGAGGAG ATCATTCTGC ACAGTCACCT GAGGAAGACA 
CACGTGCAGG CACGTCTGCA GAAACTAGAA GAGGGCACAA AATTGGACTG GTCCACCGCG 
GAGGCTCTGG CGTTTGGCAC ATTGCTATGC CAGGGATTTA ATATCCGTAT CAGTGGTCAG 
GATGTGGGTC GGGGAACGTT CAGTCAGCGC CACGCTATGG TTGTGTGTCA GGAGACCAAC 
GACATGTTCA TCCCACTCAA TCACATCAGC TCTGAACAGA AGGGCCACTT GGAGGTGTGT 
AACAGTGCTC TCTCTGAGGA AGCGGTTCTG GGTTTTGAGT ATGGGATGAG CATTGCTCAG 
CCTCGACTGC TGCCCATCTG GGAGGCTCAG TTTGGAGATT TCTTCAACGG TGCCCAGATT 
ATTTTTGATA CTTTCCTCTC TGGAGGTGAG GCTAAATGGC TCCTGCAGAG TGGACTGGTC 
ATTTTACTGC CACACGGGTA CGATGGGGCC GGTCCTGAGC ATTCATCTTG CCGAATCGAG 
CGCTTCTTAC AGCTCTGCGA CAGTAAAGAG GAAGGTGTGG ACGGGGACAC GGTCAACATG 
GCGGTGGTGA ATCCCACACT TCCAGCTCAA TACTTCCATC TTTTGAGGAG GCAGATGATC 
AGAAACTTCC GGAAACCGCT GATCGTGGCC TCTCCCAAGA CTCTGCTGCG CTTTTCTGGG 
GCAGTGTCAG GCTTGTCTGA TATGGGACCA GGAACGTCCT TTAAGCCGAT TATTGGTGAT 
TCTTCTGTGA ACCCTGCAAG TGTGCAGCGG GTTCTGTTTT GCTCAGGTAA ACATTACTAT 
GCCCTGCTGA AACACAGAGA GACGATACCT GAGGCGGAAA AAAACACAGC GCTAGTCAGG 
GTCGAGGAAC TGTGTCCTTT CCCCACAGAA GCTCTGCAAC AGGAACTAAA TAAATACACA 
AACGCCAAAG AGTTCATCTG GAGTCAAGAG GAGCCACAGA ACATGGGCTG CTGGTCATTT 
GTGTCACCGA GGTTTGAAAA ACAGTTAGCC TGCAAGTTGC GGTTGGTCAG CAGACCCGCA 
TTACCCGCTC CGGCTGTGGG CATCGGAACG CTTCACCATC AGCAGCATGA AGCCATCCTG 
ACTTCGTCTT TCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008619.1
CDS	95..2872
Translation	MSARSMLIHLPRLLHRSFGARRSFLASLYHTQRGVYGFRPRDGPERRAELTQISALNRDHGLARLVEAYRAHGHKVAKINPLLPQSPVLESVPEISLLNGAVRGVLNTTGLRHFGKAEATAEEVVAYLESTYCGGISVETSQLQTLEEREWFADRFEELKKEAFSPEERRMLAKLMLESQEFDHFLATKFATVKRYGGEGAESMMGFFYELFRSSVYSGVTDVVMGMPHRGRLNLLTGLLQFPPELMFRKMRGLSEFPEHSPSIGDVLSHLTSTVELDFGAGHPLHVTMLPNPSHLEAINPVTQGKTRGRQQVKQDGDYSTDPHSRPGDKVICLQVHGDASFSGQGIVPETFTLSNLPHYRVGGSIHLIVNNQVGYTTPSERGRSSLYCSDVGKMVGCAIIHVNGDDAEEVLRATRLAVEYQRRFRKDVIVDLLCYRQWGHNELDEPFFTNPAMYKIIRSRKSIPDSYADQLISEGLMTDEERSQIKTSYYATLNNQLTNMTLYSPPPTNLQGRWGDLVEPQNRVSCWDTGVAQPLLQFVGAKSVDIPEEIILHSHLRKTHVQARLQKLEEGTKLDWSTAEALAFGTLLCQGFNIRISGQDVGRGTFSQRHAMVVCQETNDMFIPLNHISSEQKGHLEVCNSALSEEAVLGFEYGMSIAQPRLLPIWEAQFGDFFNGAQIIFDTFLSGGEAKWLLQSGLVILLPHGYDGAGPEHSSCRIERFLQLCDSKEEGVDGDTVNMAVVNPTLPAQYFHLLRRQMIRNFRKPLIVASPKTLLRFSGAVSGLSDMGPGTSFKPIIGDSSVNPASVQRVLFCSGKHYYALLKHRETIPEAEKNTALVRVEELCPFPTEALQQELNKYTNAKEFIWSQEEPQNMGCWSFVSPRFEKQLACKLRLVSRPALPAPAVGIGTLHHQQHEAILTSSFS

Target ORF information:

RefSeq Version	NM_001008619.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dehydrogenase E1 and transketolase domain containing 1 (dhtkd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008619.1

ORF Insert Sequence:

ATGTCCGCGC GCAGTATGCT TATACACCTC CCTCGGCTTT TGCACCGCTC CTTTGGTGCC 
AGGAGGTCGT TTTTAGCGAG TTTGTACCAC ACTCAGCGTG GTGTGTACGG TTTCAGACCG 
AGAGACGGTC CAGAAAGACG GGCAGAGTTA ACGCAGATCT CCGCTCTTAA CCGAGATCAC 
GGACTGGCGA GACTGGTGGA GGCCTATCGA GCACATGGAC ACAAAGTAGC TAAAATTAAC 
CCCTTACTAC CACAAAGTCC CGTGCTGGAG AGTGTACCGG AGATAAGCCT GCTGAATGGA 
GCTGTTCGTG GAGTCCTCAA CACTACCGGG TTGCGGCACT TTGGTAAAGC TGAGGCGACT 
GCAGAAGAGG TTGTGGCGTA TCTGGAAAGC ACATACTGTG GGGGAATCTC AGTGGAAACC 
TCTCAGCTGC AGACCTTAGA GGAACGAGAA TGGTTCGCTG ACCGATTTGA GGAGCTCAAA 
AAAGAGGCGT TTTCTCCAGA GGAAAGGAGA ATGCTGGCTA AACTGATGCT CGAGTCACAG 
GAATTTGACC ACTTTTTAGC CACCAAGTTT GCAACTGTGA AGCGGTATGG AGGCGAGGGA 
GCAGAGAGCA TGATGGGATT TTTCTACGAG CTGTTTCGCT CTTCAGTATA CAGTGGAGTG 
ACGGATGTGG TCATGGGAAT GCCTCACCGC GGACGCCTTA ATCTGCTCAC CGGCCTTCTT 
CAGTTTCCTC CTGAGCTCAT GTTTCGTAAG ATGAGGGGTC TCAGTGAGTT TCCGGAGCAC 
TCTCCCTCTA TCGGAGACGT CTTGTCCCAT TTAACGTCTA CAGTGGAGCT TGACTTCGGG 
GCAGGACACC CTCTCCATGT CACAATGCTG CCCAATCCTT CCCACCTGGA GGCCATCAAC 
CCTGTGACAC AGGGGAAAAC ACGAGGCCGA CAGCAGGTCA AACAGGACGG CGACTACTCC 
ACTGACCCTC ATTCACGACC GGGAGACAAA GTCATCTGCC TGCAGGTTCA TGGCGATGCT 
TCATTTTCAG GACAAGGAAT AGTACCGGAA ACCTTCACCT TGTCAAATCT CCCTCATTAT 
AGAGTGGGTG GAAGCATCCA CCTCATTGTA AACAATCAAG TGGGCTACAC CACTCCCTCT 
GAGAGGGGAA GATCATCTCT CTACTGTAGT GATGTTGGTA AAATGGTGGG ATGTGCAATA 
ATCCATGTGA ATGGCGATGA TGCTGAAGAA GTCCTCAGAG CGACCCGGCT GGCGGTGGAG 
TACCAGAGAC GCTTCAGAAA AGACGTCATT GTTGATCTGC TTTGTTACCG GCAGTGGGGC 
CACAATGAGC TGGACGAACC CTTTTTCACA AACCCTGCAA TGTATAAAAT AATACGGTCA 
AGAAAAAGCA TCCCAGATTC CTATGCTGAC CAGCTGATCT CTGAGGGTCT GATGACAGAC 
GAGGAGCGCA GTCAGATCAA GACCAGCTAC TACGCTACGC TCAACAACCA GCTGACTAAC 
ATGACCCTCT ACAGCCCTCC ACCCACAAAC CTGCAGGGCC GCTGGGGAGA CCTGGTGGAG 
CCCCAGAATA GAGTCTCGTG CTGGGACACT GGCGTCGCAC AGCCCCTGCT GCAGTTTGTT 
GGAGCCAAAT CAGTGGACAT ACCTGAGGAG ATCATTCTGC ACAGTCACCT GAGGAAGACA 
CACGTGCAGG CACGTCTGCA GAAACTAGAA GAGGGCACAA AATTGGACTG GTCCACCGCG 
GAGGCTCTGG CGTTTGGCAC ATTGCTATGC CAGGGATTTA ATATCCGTAT CAGTGGTCAG 
GATGTGGGTC GGGGAACGTT CAGTCAGCGC CACGCTATGG TTGTGTGTCA GGAGACCAAC 
GACATGTTCA TCCCACTCAA TCACATCAGC TCTGAACAGA AGGGCCACTT GGAGGTGTGT 
AACAGTGCTC TCTCTGAGGA AGCGGTTCTG GGTTTTGAGT ATGGGATGAG CATTGCTCAG 
CCTCGACTGC TGCCCATCTG GGAGGCTCAG TTTGGAGATT TCTTCAACGG TGCCCAGATT 
ATTTTTGATA CTTTCCTCTC TGGAGGTGAG GCTAAATGGC TCCTGCAGAG TGGACTGGTC 
ATTTTACTGC CACACGGGTA CGATGGGGCC GGTCCTGAGC ATTCATCTTG CCGAATCGAG 
CGCTTCTTAC AGCTCTGCGA CAGTAAAGAG GAAGGTGTGG ACGGGGACAC GGTCAACATG 
GCGGTGGTGA ATCCCACACT TCCAGCTCAA TACTTCCATC TTTTGAGGAG GCAGATGATC 
AGAAACTTCC GGAAACCGCT GATCGTGGCC TCTCCCAAGA CTCTGCTGCG CTTTTCTGGG 
GCAGTGTCAG GCTTGTCTGA TATGGGACCA GGAACGTCCT TTAAGCCGAT TATTGGTGAT 
TCTTCTGTGA ACCCTGCAAG TGTGCAGCGG GTTCTGTTTT GCTCAGGTAA ACATTACTAT 
GCCCTGCTGA AACACAGAGA GACGATACCT GAGGCGGAAA AAAACACAGC GCTAGTCAGG 
GTCGAGGAAC TGTGTCCTTT CCCCACAGAA GCTCTGCAAC AGGAACTAAA TAAATACACA 
AACGCCAAAG AGTTCATCTG GAGTCAAGAG GAGCCACAGA ACATGGGCTG CTGGTCATTT 
GTGTCACCGA GGTTTGAAAA ACAGTTAGCC TGCAAGTTGC GGTTGGTCAG CAGACCCGCA 
TTACCCGCTC CGGCTGTGGG CATCGGAACG CTTCACCATC AGCAGCATGA AGCCATCCTG 
ACTTCGTCTT TCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A ?Raf1-ER-inducible oncogenic zebrafish liver cell model identifies hepatocellular carcinoma signatures.
The Journal of pathology225(1)19-28(2011 Sep)
He S,Krens SG,Zhan H,Gong Z,Hogendoorn PC,Spaink HP,Snaar-Jagalska BE

Identification and expression of liver-specific genes after LPS challenge in amphioxus: the hepatic cecum as liver-like organ and "pre-hepatic" acute phase response.
Functional & integrative genomics11(1)111-8(2011 Mar)
Wang Y,Zhang S