DHTKD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DHTKD1
Entrez Gene ID	55526
Full Name	dehydrogenase E1 and transketolase domain containing 1
Synonyms	AMOXAD,CMT2Q
General protein information	Preferred Names dehydrogenase E1 and transketolase domain containing 1 Names probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial dehydrogenase E1 and transketolase domain-containing protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10p14
Summary	This gene encodes a component of a mitochondrial 2-oxoglutarate-dehydrogenase-complex-like protein involved in the degradation pathways of several amino acids, including lysine. Mutations in this gene are associated with 2-aminoadipic 2-oxoadipic aciduria and Charcot-Marie-Tooth Disease Type 2Q. [provided by RefSeq, May 2013].
Disorder MIM:	614984
Disorder Html:	2-aminoadipic 2-oxoadipic aciduria, 204750 (3); ?Charcot-Marie-Tooth disease, axonal, type 2Q, 615025 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_018706.6	NP_061176.3	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
NM_018706.7	NP_061176.4	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DHTKD1	XP_001135864.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000551	XP_310532.5
	LOC696445	XP_001085071.2
Mus musculus (house mouse)	Dhtkd1	NP_001074600.1
Bos taurus (cattle)	DHTKD1	NP_001192767.1
Rattus norvegicus (Norway rat)	Dhtkd1	NP_001020891.1
Danio rerio (zebrafish)	dhtkd1	NP_001008619.1
Homo sapiens (human)	DHTKD1	NP_061176.3
Canis lupus familiaris (dog)	DHTKD1	XP_535192.3
Gallus gallus (chicken)	DHTKD1	XP_004937543.1
Drosophila melanogaster (fruit fly)	CG1544	NP_651849.1
Caenorhabditis elegans (roundworm)	ZK836.2	NP_001256410.1
Xenopus tropicalis (tropical clawed frog)	dhtkd1	XP_002931921.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic basis of alpha-aminoadipic and alpha-ketoadipic aciduria.
Hagen J, te Brinke H, Wanders RJ, Knegt AC, Oussoren E, Hoogeboom AJ, Ruijter GJ, Becker D, Schwab KO, Franke I, Duran M, Waterham HR, Sass JO, Houten SM
Journal of inherited metabolic disease38(5)873-9(2015 Sep)

DHTKD1 is essential for mitochondrial biogenesis and function maintenance.
Xu W, Zhu H, Gu M, Luo Q, Ding J, Yao Y, Chen F, Wang Z
FEBS letters587(21)3587-92(2013 Nov)

DHTKD1 mutations cause 2-aminoadipic and 2-oxoadipic aciduria.
Danhauser K, Sauer SW, Haack TB, Wieland T, Staufner C, Graf E, Zschocke J, Strom TM, Traub T, Okun JG, Meitinger T, Hoffmann GF, Prokisch H, K?lker S
American journal of human genetics91(6)1082-7(2012 Dec)

A nonsense mutation in DHTKD1 causes Charcot-Marie-Tooth disease type 2 in a large Chinese pedigree.
Xu WY, Gu MM, Sun LH, Guo WT, Zhu HB, Ma JF, Yuan WT, Kuang Y, Ji BJ, Wu XL, Chen Y, Zhang HX, Sun FT, Huang W, Huang L, Chen SD, Wang ZG
American journal of human genetics91(6)1088-94(2012 Dec)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DHTKD1 encodes the E1 subunit of the alpha-ketoadipic acid dehydrogenase complex
Title: Genetic basis of alpha-aminoadipic and alpha-ketoadipic aciduria.

DHTKD1 contributes to mitochondrial biogenesis and function maintenance.
Title: DHTKD1 is essential for mitochondrial biogenesis and function maintenance.

DHTKD1 mutations cause 2-aminoadipic and 2-oxoadipic aciduria.
Title: DHTKD1 mutations cause 2-aminoadipic and 2-oxoadipic aciduria.

A nonsense mutation in DHTKD1 causes Charcot-Marie-Tooth disease type 2 in a large Chinese pedigree.
Title: A nonsense mutation in DHTKD1 causes Charcot-Marie-Tooth disease type 2 in a large Chinese pedigree.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following DHTKD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHTKD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu21445	NM_018706.6 Latest version!	Homo sapiens dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu107795	NM_018706.7 Latest version!	Homo sapiens dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DHTKD1 ( NM_018706.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_061176.3 Homo sapiens dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), mRNA.

Price & Availability↑

CloneID	OHu21445
Clone ID Related Accession (Same CDS sequence)	NM_018706.6
Accession Version	NM_018706.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY039281.1, BC007955.2, AC073160.8, AI823832.1 and AW083293.1. This sequence is a reference standard in the RefSeqGene project. On May 16, 2013 this sequence version replaced NM_018706.5. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.207230.1, SRR1803615.247322.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCTG CTACTGCGGC AGCAGCACGA CGGGGCCTCG GCCGGGCTCT CCCTCTCCTC 
TGGCGTGGCT ACCAGACCGA GCGGGGCGTT TACGGCTACC GGCCGAGGAA GCCCGAGAGC 
CGCGAGCCCC AGGGCGCCCT GGAGCGCCCC CCAGTTGATC ATGGCCTTGC CAGGTTGGTG 
ACAGTATATT GTGAGCATGG TCATAAAGCT GCCAAAATCA ACCCCCTCTT CACCGGACAA 
GCCCTGCTGG AGAATGTGCC TGAAATCCAA GCCCTGGTGC AGACACTGCA GGGACCCTTC 
CACACGGCAG GATTATTGAA CATGGGGAAG GAAGAGGCCT CACTTGAGGA AGTGTTAGTC 
TATCTCAATC AAATCTACTG TGGGCAGATT TCTATTGAAA CCTCCCAACT TCAGAGCCAG 
GATGAGAAAG ACTGGTTTGC CAAGCGGTTT GAGGAACTGC AAAAGGAGAC GTTTACCACA 
GAAGAGCGAA AACATCTGTC GAAACTAATG CTGGAATCTC AGGAGTTTGA CCACTTTCTG 
GCCACCAAGT TCTCGACAGT GAAGCGATAT GGAGGCGAAG GGGCTGAAAG CATGATGGGC 
TTTTTCCACG AGCTGCTGAA AATGTCGGCC TACAGCGGGA TCACTGATGT CATTATTGGG 
ATGCCCCATA GAGGGAGGCT GAATTTATTG ACAGGCCTTC TGCAGTTCCC TCCAGAGCTG 
ATGTTCCGTA AAATGCGAGG CTTAAGTGAA TTTCCAGAGA ATTTCTCAGC CACTGGAGAC 
GTCCTGTCTC ACCTGACCTC CTCTGTGGAC CTGTACTTTG GGGCGCACCA TCCCCTCCAT 
GTGACAATGT TGCCCAATCC CTCGCACCTG GAGGCCGTCA ACCCCGTGGC CGTGGGCAAA 
ACTCGCGGCA GGCAGCAGTC TCGCCAAGAC GGCGATTACT CTCCAGACAA CTCAGCCCAG 
CCGGGGGACA GGGTCATTTG CTTACAGGTC CATGGTGATG CTTCTTTCTG TGGTCAAGGG 
ATTGTTCCTG AAACATTCAC GCTGTCCAAT CTCCCACATT TCAGAATTGG TGGGAGTGTG 
CATTTGATTG TTAATAACCA GCTGGGTTAC ACCACTCCAG CTGAAAGAGG AAGGTCTTCT 
TTATACTGCA GTGATATTGG GAAGCTTGTG GGCTGTGCCA TCATCCATGT CAATGGAGAC 
AGCCCAGAGG AAGTGGTCCG TGCCACACGA CTGGCTTTTG AATACCAACG CCAGTTCCGC 
AAGGATGTGA TTATTGATCT GTTGTGCTAC AGGCAGTGGG GCCACAATGA GCTGGATGAG 
CCATTCTACA CCAACCCCAT CATGTACAAA ATCATCAGAG CTCGAAAGAG CATTCCAGAC 
ACATATGCAG AGCACCTCAT TGCTGGCGGA CTCATGACGC AGGAGGAGGT GTCTGAAATA 
AAATCCTCCT ACTATGCCAA GTTGAATGAT CACTTAAATA ACATGGCCCA CTACAGGCCC 
CCTGCCCTGA ACCTGCAGGC CCACTGGCAG GGCCTGGCTC AGCCAGAAGC GCAAATCACC 
ACCTGGAGTA CAGGTGTGCC CCTCGACCTC CTGCGGTTTG TTGGCATGAA GTCTGTAGAG 
GTGCCAAGAG AGCTGCAGAT GCACAGTCAC CTGCTGAAGA CACATGTTCA GTCCAGAATG 
GAGAAGATGA TGGACGGAAT CAAGCTAGAC TGGGCCACCG CGGAAGCTCT TGCCTTGGGT 
TCTTTACTTG CTCAAGGTTT TAATGTTCGT CTAAGTGGCC AAGATGTTGG TCGTGGAACT 
TTCAGTCAGA GGCATGCAAT CGTGGTTTGC CAGGAGACGG ATGACACCTA CATCCCCCTG 
AACCATATGG ACCCAAATCA GAAGGGGTTT CTAGAGGTCA GCAACAGCCC CCTGTCAGAA 
GAGGCCGTCC TGGGATTCGA ATATGGGATG AGCATTGAGA GCCCAAAGTT ACTGCCCCTG 
TGGGAGGCAC AGTTTGGCGA TTTCTTCAAT GGTGCCCAGA TCATCTTTGA CACATTCATC 
TCTGGAGGAG AGGCCAAGTG GCTCCTACAA AGCGGCATCG TCATCCTCCT TCCACATGGC 
TACGATGGGG CTGGGCCAGA CCACTCATCC TGTCGAATAG AGCGTTTCCT GCAGATGTGT 
GACAGTGCGG AAGAGGGGGT GGACGGAGAC ACTGTGAACA TGTTTGTGGT TCACCCAACA 
ACTCCTGCAC AGTATTTCCA CTTGCTTAGG AGACAGATGG TCCGGAACTT CAGAAAACCA 
CTCATTGTTG CTTCCCCTAA GATGTTACTC AGGCTCCCGG CAGCCGTGTC AACTCTTCAA 
GAAATGGCAC CAGGAACAAC ATTTAACCCG GTCATTGGTG ATTCATCTGT GGATCCAAAA 
AAGGTTAAGA CCCTCGTGTT CTGCTCCGGC AAACATTTCT ACTCCCTGGT GAAACAAAGA 
GAATCTCTGG GGGCCAAGAA GCATGACTTT GCCATCATCC GAGTAGAGGA ACTCTGCCCC 
TTCCCGTTGG ATTCTTTACA GCAAGAGATG AGCAAATACA AACATGTTAA AGATCATATT 
TGGAGTCAGG AGGAACCTCA GAACATGGGT CCGTGGTCGT TTGTTTCTCC AAGGTTTGAA 
AAGCAGCTGG CCTGCAAGCT CCGTCTGGTG GGCCGGCCCC CTTTGCCAGT ACCCGCTGTA 
GGAATTGGCA CAGTTCACTT GCACCAGCAT GAAGATATCC TCGCCAAGAC CTTCGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061176.3
CDS	118..2877
Translation	MASATAAAARRGLGRALPLLWRGYQTERGVYGYRPRKPESREPQGALERPPVDHGLARLVTVYCEHGHKAAKINPLFTGQALLENVPEIQALVQTLQGPFHTAGLLNMGKEEASLEEVLVYLNQIYCGQISIETSQLQSQDEKDWFAKRFEELQKETFTTEERKHLSKLMLESQEFDHFLATKFSTVKRYGGEGAESMMGFFHELLKMSAYSGITDVIIGMPHRGRLNLLTGLLQFPPELMFRKMRGLSEFPENFSATGDVLSHLTSSVDLYFGAHHPLHVTMLPNPSHLEAVNPVAVGKTRGRQQSRQDGDYSPDNSAQPGDRVICLQVHGDASFCGQGIVPETFTLSNLPHFRIGGSVHLIVNNQLGYTTPAERGRSSLYCSDIGKLVGCAIIHVNGDSPEEVVRATRLAFEYQRQFRKDVIIDLLCYRQWGHNELDEPFYTNPIMYKIIRARKSIPDTYAEHLIAGGLMTQEEVSEIKSSYYAKLNDHLNNMAHYRPPALNLQAHWQGLAQPEAQITTWSTGVPLDLLRFVGMKSVEVPRELQMHSHLLKTHVQSRMEKMMDGIKLDWATAEALALGSLLAQGFNVRLSGQDVGRGTFSQRHAIVVCQETDDTYIPLNHMDPNQKGFLEVSNSPLSEEAVLGFEYGMSIESPKLLPLWEAQFGDFFNGAQIIFDTFISGGEAKWLLQSGIVILLPHGYDGAGPDHSSCRIERFLQMCDSAEEGVDGDTVNMFVVHPTTPAQYFHLLRRQMVRNFRKPLIVASPKMLLRLPAAVSTLQEMAPGTTFNPVIGDSSVDPKKVKTLVFCSGKHFYSLVKQRESLGAKKHDFAIIRVEELCPFPLDSLQQEMSKYKHVKDHIWSQEEPQNMGPWSFVSPRFEKQLACKLRLVGRPPLPVPAVGIGTVHLHQHEDILAKTFA

Target ORF information:

RefSeq Version	NM_018706.6
Organism	Homo sapiens(human)
Definition	Homo sapiens dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018706.6

ORF Insert Sequence:

ATGGCCTCTG CTACTGCGGC AGCAGCACGA CGGGGCCTCG GCCGGGCTCT CCCTCTCCTC 
TGGCGTGGCT ACCAGACCGA GCGGGGCGTT TACGGCTACC GGCCGAGGAA GCCCGAGAGC 
CGCGAGCCCC AGGGCGCCCT GGAGCGCCCC CCAGTTGATC ATGGCCTTGC CAGGTTGGTG 
ACAGTATATT GTGAGCATGG TCATAAAGCT GCCAAAATCA ACCCCCTCTT CACCGGACAA 
GCCCTGCTGG AGAATGTGCC TGAAATCCAA GCCCTGGTGC AGACACTGCA GGGACCCTTC 
CACACGGCAG GATTATTGAA CATGGGGAAG GAAGAGGCCT CACTTGAGGA AGTGTTAGTC 
TATCTCAATC AAATCTACTG TGGGCAGATT TCTATTGAAA CCTCCCAACT TCAGAGCCAG 
GATGAGAAAG ACTGGTTTGC CAAGCGGTTT GAGGAACTGC AAAAGGAGAC GTTTACCACA 
GAAGAGCGAA AACATCTGTC GAAACTAATG CTGGAATCTC AGGAGTTTGA CCACTTTCTG 
GCCACCAAGT TCTCGACAGT GAAGCGATAT GGAGGCGAAG GGGCTGAAAG CATGATGGGC 
TTTTTCCACG AGCTGCTGAA AATGTCGGCC TACAGCGGGA TCACTGATGT CATTATTGGG 
ATGCCCCATA GAGGGAGGCT GAATTTATTG ACAGGCCTTC TGCAGTTCCC TCCAGAGCTG 
ATGTTCCGTA AAATGCGAGG CTTAAGTGAA TTTCCAGAGA ATTTCTCAGC CACTGGAGAC 
GTCCTGTCTC ACCTGACCTC CTCTGTGGAC CTGTACTTTG GGGCGCACCA TCCCCTCCAT 
GTGACAATGT TGCCCAATCC CTCGCACCTG GAGGCCGTCA ACCCCGTGGC CGTGGGCAAA 
ACTCGCGGCA GGCAGCAGTC TCGCCAAGAC GGCGATTACT CTCCAGACAA CTCAGCCCAG 
CCGGGGGACA GGGTCATTTG CTTACAGGTC CATGGTGATG CTTCTTTCTG TGGTCAAGGG 
ATTGTTCCTG AAACATTCAC GCTGTCCAAT CTCCCACATT TCAGAATTGG TGGGAGTGTG 
CATTTGATTG TTAATAACCA GCTGGGTTAC ACCACTCCAG CTGAAAGAGG AAGGTCTTCT 
TTATACTGCA GTGATATTGG GAAGCTTGTG GGCTGTGCCA TCATCCATGT CAATGGAGAC 
AGCCCAGAGG AAGTGGTCCG TGCCACACGA CTGGCTTTTG AATACCAACG CCAGTTCCGC 
AAGGATGTGA TTATTGATCT GTTGTGCTAC AGGCAGTGGG GCCACAATGA GCTGGATGAG 
CCATTCTACA CCAACCCCAT CATGTACAAA ATCATCAGAG CTCGAAAGAG CATTCCAGAC 
ACATATGCAG AGCACCTCAT TGCTGGCGGA CTCATGACGC AGGAGGAGGT GTCTGAAATA 
AAATCCTCCT ACTATGCCAA GTTGAATGAT CACTTAAATA ACATGGCCCA CTACAGGCCC 
CCTGCCCTGA ACCTGCAGGC CCACTGGCAG GGCCTGGCTC AGCCAGAAGC GCAAATCACC 
ACCTGGAGTA CAGGTGTGCC CCTCGACCTC CTGCGGTTTG TTGGCATGAA GTCTGTAGAG 
GTGCCAAGAG AGCTGCAGAT GCACAGTCAC CTGCTGAAGA CACATGTTCA GTCCAGAATG 
GAGAAGATGA TGGACGGAAT CAAGCTAGAC TGGGCCACCG CGGAAGCTCT TGCCTTGGGT 
TCTTTACTTG CTCAAGGTTT TAATGTTCGT CTAAGTGGCC AAGATGTTGG TCGTGGAACT 
TTCAGTCAGA GGCATGCAAT CGTGGTTTGC CAGGAGACGG ATGACACCTA CATCCCCCTG 
AACCATATGG ACCCAAATCA GAAGGGGTTT CTAGAGGTCA GCAACAGCCC CCTGTCAGAA 
GAGGCCGTCC TGGGATTCGA ATATGGGATG AGCATTGAGA GCCCAAAGTT ACTGCCCCTG 
TGGGAGGCAC AGTTTGGCGA TTTCTTCAAT GGTGCCCAGA TCATCTTTGA CACATTCATC 
TCTGGAGGAG AGGCCAAGTG GCTCCTACAA AGCGGCATCG TCATCCTCCT TCCACATGGC 
TACGATGGGG CTGGGCCAGA CCACTCATCC TGTCGAATAG AGCGTTTCCT GCAGATGTGT 
GACAGTGCGG AAGAGGGGGT GGACGGAGAC ACTGTGAACA TGTTTGTGGT TCACCCAACA 
ACTCCTGCAC AGTATTTCCA CTTGCTTAGG AGACAGATGG TCCGGAACTT CAGAAAACCA 
CTCATTGTTG CTTCCCCTAA GATGTTACTC AGGCTCCCGG CAGCCGTGTC AACTCTTCAA 
GAAATGGCAC CAGGAACAAC ATTTAACCCG GTCATTGGTG ATTCATCTGT GGATCCAAAA 
AAGGTTAAGA CCCTCGTGTT CTGCTCCGGC AAACATTTCT ACTCCCTGGT GAAACAAAGA 
GAATCTCTGG GGGCCAAGAA GCATGACTTT GCCATCATCC GAGTAGAGGA ACTCTGCCCC 
TTCCCGTTGG ATTCTTTACA GCAAGAGATG AGCAAATACA AACATGTTAA AGATCATATT 
TGGAGTCAGG AGGAACCTCA GAACATGGGT CCGTGGTCGT TTGTTTCTCC AAGGTTTGAA 
AAGCAGCTGG CCTGCAAGCT CCGTCTGGTG GGCCGGCCCC CTTTGCCAGT ACCCGCTGTA 
GGAATTGGCA CAGTTCACTT GCACCAGCAT GAAGATATCC TCGCCAAGAC CTTCGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHTKD1 ( NM_018706.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_061176.4 Homo sapiens dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), mRNA.

Price & Availability↑

CloneID	OHu107795
Clone ID Related Accession (Same CDS sequence)	NM_018706.7
Accession Version	NM_018706.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC073160.8. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_018706.6. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.121170.1, SRR1803615.207230.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000263035.9/ ENSP00000263035.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCTCTG CTACTGCGGC AGCAGCACGA CGGGGCCTCG GCCGGGCTCT CCCTCTCTTC 
TGGCGTGGCT ACCAGACCGA GCGGGGCGTT TACGGCTACC GGCCGAGGAA GCCCGAGAGC 
CGCGAGCCCC AGGGCGCCCT GGAGCGCCCC CCAGTTGATC ATGGCCTTGC CAGGTTGGTG 
ACAGTATATT GTGAGCATGG TCATAAAGCT GCCAAAATCA ACCCCCTCTT CACCGGACAA 
GCCCTGCTGG AGAATGTGCC TGAAATCCAA GCCCTGGTGC AGACACTGCA GGGACCCTTC 
CACACGGCAG GATTATTGAA CATGGGGAAG GAAGAGGCCT CACTTGAGGA AGTGTTAGTC 
TATCTCAATC AAATCTACTG TGGGCAGATT TCTATTGAAA CCTCCCAACT TCAGAGCCAG 
GATGAGAAAG ACTGGTTTGC CAAGCGGTTT GAGGAACTGC AAAAGGAGAC GTTTACCACA 
GAAGAGCGAA AACATCTGTC GAAACTAATG CTGGAATCTC AGGAGTTTGA CCACTTTCTG 
GCCACCAAGT TCTCGACAGT GAAGCGATAT GGAGGCGAAG GGGCTGAAAG CATGATGGGC 
TTTTTCCACG AGCTGCTGAA AATGTCGGCC TACAGCGGGA TCACTGATGT CATTATTGGG 
ATGCCCCATA GAGGGAGGCT GAATTTATTG ACAGGCCTTC TGCAGTTCCC TCCAGAGCTG 
ATGTTCCGTA AAATGCGAGG CTTAAGTGAA TTTCCAGAGA ATTTCTCAGC CACTGGAGAC 
GTCCTGTCTC ACCTGACCTC CTCTGTGGAC CTGTACTTTG GGGCGCACCA TCCCCTCCAT 
GTGACAATGT TGCCCAATCC CTCGCACCTG GAGGCCGTCA ACCCCGTGGC CGTGGGCAAA 
ACTCGCGGCA GGCAGCAGTC TCGCCAAGAC GGCGATTACT CTCCAGACAA CTCAGCCCAG 
CCGGGGGACA GGGTCATTTG CTTACAGGTC CATGGTGATG CTTCTTTCTG TGGTCAAGGG 
ATTGTTCCTG AAACATTCAC GCTGTCCAAT CTCCCACATT TCAGAATTGG TGGGAGTGTG 
CATTTGATTG TTAATAACCA GCTGGGTTAC ACCACTCCAG CTGAAAGAGG AAGGTCTTCT 
TTATACTGCA GTGATATTGG GAAGCTTGTG GGCTGTGCCA TCATCCATGT CAATGGAGAC 
AGCCCAGAGG AAGTGGTCCG TGCCACACGA CTGGCTTTTG AATACCAACG CCAGTTCCGC 
AAGGATGTGA TTATTGATCT GTTGTGCTAC AGGCAGTGGG GCCACAATGA GCTGGATGAG 
CCATTCTACA CCAACCCCAT CATGTACAAA ATCATCAGAG CTCGAAAGAG CATTCCAGAC 
ACATATGCAG AGCACCTCAT TGCTGGCGGA CTCATGACGC AGGAGGAGGT GTCTGAAATA 
AAATCCTCCT ACTATGCCAA GTTGAATGAT CACTTAAATA ACATGGCCCA CTACAGGCCC 
CCTGCCCTGA ACCTGCAGGC CCACTGGCAG GGCCTGGCTC AGCCAGAAGC GCAAATCACC 
ACCTGGAGTA CAGGTGTGCC CCTCGACCTC CTGCGGTTTG TTGGCATGAA GTCTGTAGAG 
GTGCCAAGAG AGCTGCAGAT GCACAGTCAC CTGCTGAAGA CACATGTTCA GTCCAGAATG 
GAGAAGATGA TGGACGGAAT CAAGCTAGAC TGGGCCACCG CGGAAGCTCT TGCCTTGGGT 
TCTTTACTTG CTCAAGGTTT TAATGTTCGT CTAAGTGGCC AAGATGTTGG TCGTGGAACT 
TTCAGTCAGA GGCATGCAAT CGTGGTTTGC CAGGAGACGG ATGACACCTA CATCCCCCTG 
AACCATATGG ACCCAAATCA GAAGGGGTTT CTAGAGGTCA GCAACAGCCC ACTGTCAGAA 
GAGGCCGTCC TGGGATTTGA ATATGGGATG AGCATTGAGA GCCCAAAGTT ACTGCCCCTG 
TGGGAGGCAC AGTTTGGCGA TTTCTTCAAT GGTGCCCAGA TCATCTTTGA CACATTCATC 
TCTGGAGGAG AGGCCAAGTG GCTCCTACAA AGCGGCATCG TCATCCTCCT TCCACATGGC 
TACGATGGGG CTGGGCCAGA CCACTCATCC TGTCGAATAG AGCGTTTCCT GCAGATGTGT 
GACAGTGCGG AAGAGGGGGT GGACGGAGAC ACTGTGAACA TGTTTGTGGT TCACCCAACA 
ACTCCTGCAC AGTATTTCCA CTTGCTTAGG AGACAGATGG TCCGGAACTT CAGAAAACCA 
CTCATTGTTG CTTCCCCTAA GATGTTACTC AGGCTCCCGG CAGCCGTGTC AACTCTTCAA 
GAAATGGCAC CAGGAACAAC ATTTAACCCG GTCATTGGTG ATTCATCTGT GGATCCAAAA 
AAGGTTAAGA CCCTCGTGTT CTGCTCCGGC AAACATTTCT ACTCCCTGGT GAAACAAAGA 
GAATCTCTGG GGGCCAAGAA GCATGACTTT GCCATCATCC GAGTAGAGGA ACTCTGCCCC 
TTCCCGTTGG ATTCTTTACA GCAAGAGATG AGCAAATACA AACATGTTAA AGATCATATT 
TGGAGTCAGG AGGAACCTCA GAACATGGGT CCGTGGTCGT TTGTTTCTCC AAGGTTTGAA 
AAGCAGCTGG CCTGCAAGCT CCGTCTGGTG GGCCGGCCCC CTTTGCCAGT ACCCGCTGTA 
GGAATTGGCA CAGTTCACTT GCACCAGCAT GAAGATATCC TCGCCAAGAC CTTCGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061176.4
CDS	81..2840
Translation	MASATAAAARRGLGRALPLFWRGYQTERGVYGYRPRKPESREPQGALERPPVDHGLARLVTVYCEHGHKAAKINPLFTGQALLENVPEIQALVQTLQGPFHTAGLLNMGKEEASLEEVLVYLNQIYCGQISIETSQLQSQDEKDWFAKRFEELQKETFTTEERKHLSKLMLESQEFDHFLATKFSTVKRYGGEGAESMMGFFHELLKMSAYSGITDVIIGMPHRGRLNLLTGLLQFPPELMFRKMRGLSEFPENFSATGDVLSHLTSSVDLYFGAHHPLHVTMLPNPSHLEAVNPVAVGKTRGRQQSRQDGDYSPDNSAQPGDRVICLQVHGDASFCGQGIVPETFTLSNLPHFRIGGSVHLIVNNQLGYTTPAERGRSSLYCSDIGKLVGCAIIHVNGDSPEEVVRATRLAFEYQRQFRKDVIIDLLCYRQWGHNELDEPFYTNPIMYKIIRARKSIPDTYAEHLIAGGLMTQEEVSEIKSSYYAKLNDHLNNMAHYRPPALNLQAHWQGLAQPEAQITTWSTGVPLDLLRFVGMKSVEVPRELQMHSHLLKTHVQSRMEKMMDGIKLDWATAEALALGSLLAQGFNVRLSGQDVGRGTFSQRHAIVVCQETDDTYIPLNHMDPNQKGFLEVSNSPLSEEAVLGFEYGMSIESPKLLPLWEAQFGDFFNGAQIIFDTFISGGEAKWLLQSGIVILLPHGYDGAGPDHSSCRIERFLQMCDSAEEGVDGDTVNMFVVHPTTPAQYFHLLRRQMVRNFRKPLIVASPKMLLRLPAAVSTLQEMAPGTTFNPVIGDSSVDPKKVKTLVFCSGKHFYSLVKQRESLGAKKHDFAIIRVEELCPFPLDSLQQEMSKYKHVKDHIWSQEEPQNMGPWSFVSPRFEKQLACKLRLVGRPPLPVPAVGIGTVHLHQHEDILAKTFA

Target ORF information:

RefSeq Version	NM_018706.7
Organism	Homo sapiens(human)
Definition	Homo sapiens dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018706.7

ORF Insert Sequence:

ATGGCCTCTG CTACTGCGGC AGCAGCACGA CGGGGCCTCG GCCGGGCTCT CCCTCTCTTC 
TGGCGTGGCT ACCAGACCGA GCGGGGCGTT TACGGCTACC GGCCGAGGAA GCCCGAGAGC 
CGCGAGCCCC AGGGCGCCCT GGAGCGCCCC CCAGTTGATC ATGGCCTTGC CAGGTTGGTG 
ACAGTATATT GTGAGCATGG TCATAAAGCT GCCAAAATCA ACCCCCTCTT CACCGGACAA 
GCCCTGCTGG AGAATGTGCC TGAAATCCAA GCCCTGGTGC AGACACTGCA GGGACCCTTC 
CACACGGCAG GATTATTGAA CATGGGGAAG GAAGAGGCCT CACTTGAGGA AGTGTTAGTC 
TATCTCAATC AAATCTACTG TGGGCAGATT TCTATTGAAA CCTCCCAACT TCAGAGCCAG 
GATGAGAAAG ACTGGTTTGC CAAGCGGTTT GAGGAACTGC AAAAGGAGAC GTTTACCACA 
GAAGAGCGAA AACATCTGTC GAAACTAATG CTGGAATCTC AGGAGTTTGA CCACTTTCTG 
GCCACCAAGT TCTCGACAGT GAAGCGATAT GGAGGCGAAG GGGCTGAAAG CATGATGGGC 
TTTTTCCACG AGCTGCTGAA AATGTCGGCC TACAGCGGGA TCACTGATGT CATTATTGGG 
ATGCCCCATA GAGGGAGGCT GAATTTATTG ACAGGCCTTC TGCAGTTCCC TCCAGAGCTG 
ATGTTCCGTA AAATGCGAGG CTTAAGTGAA TTTCCAGAGA ATTTCTCAGC CACTGGAGAC 
GTCCTGTCTC ACCTGACCTC CTCTGTGGAC CTGTACTTTG GGGCGCACCA TCCCCTCCAT 
GTGACAATGT TGCCCAATCC CTCGCACCTG GAGGCCGTCA ACCCCGTGGC CGTGGGCAAA 
ACTCGCGGCA GGCAGCAGTC TCGCCAAGAC GGCGATTACT CTCCAGACAA CTCAGCCCAG 
CCGGGGGACA GGGTCATTTG CTTACAGGTC CATGGTGATG CTTCTTTCTG TGGTCAAGGG 
ATTGTTCCTG AAACATTCAC GCTGTCCAAT CTCCCACATT TCAGAATTGG TGGGAGTGTG 
CATTTGATTG TTAATAACCA GCTGGGTTAC ACCACTCCAG CTGAAAGAGG AAGGTCTTCT 
TTATACTGCA GTGATATTGG GAAGCTTGTG GGCTGTGCCA TCATCCATGT CAATGGAGAC 
AGCCCAGAGG AAGTGGTCCG TGCCACACGA CTGGCTTTTG AATACCAACG CCAGTTCCGC 
AAGGATGTGA TTATTGATCT GTTGTGCTAC AGGCAGTGGG GCCACAATGA GCTGGATGAG 
CCATTCTACA CCAACCCCAT CATGTACAAA ATCATCAGAG CTCGAAAGAG CATTCCAGAC 
ACATATGCAG AGCACCTCAT TGCTGGCGGA CTCATGACGC AGGAGGAGGT GTCTGAAATA 
AAATCCTCCT ACTATGCCAA GTTGAATGAT CACTTAAATA ACATGGCCCA CTACAGGCCC 
CCTGCCCTGA ACCTGCAGGC CCACTGGCAG GGCCTGGCTC AGCCAGAAGC GCAAATCACC 
ACCTGGAGTA CAGGTGTGCC CCTCGACCTC CTGCGGTTTG TTGGCATGAA GTCTGTAGAG 
GTGCCAAGAG AGCTGCAGAT GCACAGTCAC CTGCTGAAGA CACATGTTCA GTCCAGAATG 
GAGAAGATGA TGGACGGAAT CAAGCTAGAC TGGGCCACCG CGGAAGCTCT TGCCTTGGGT 
TCTTTACTTG CTCAAGGTTT TAATGTTCGT CTAAGTGGCC AAGATGTTGG TCGTGGAACT 
TTCAGTCAGA GGCATGCAAT CGTGGTTTGC CAGGAGACGG ATGACACCTA CATCCCCCTG 
AACCATATGG ACCCAAATCA GAAGGGGTTT CTAGAGGTCA GCAACAGCCC ACTGTCAGAA 
GAGGCCGTCC TGGGATTTGA ATATGGGATG AGCATTGAGA GCCCAAAGTT ACTGCCCCTG 
TGGGAGGCAC AGTTTGGCGA TTTCTTCAAT GGTGCCCAGA TCATCTTTGA CACATTCATC 
TCTGGAGGAG AGGCCAAGTG GCTCCTACAA AGCGGCATCG TCATCCTCCT TCCACATGGC 
TACGATGGGG CTGGGCCAGA CCACTCATCC TGTCGAATAG AGCGTTTCCT GCAGATGTGT 
GACAGTGCGG AAGAGGGGGT GGACGGAGAC ACTGTGAACA TGTTTGTGGT TCACCCAACA 
ACTCCTGCAC AGTATTTCCA CTTGCTTAGG AGACAGATGG TCCGGAACTT CAGAAAACCA 
CTCATTGTTG CTTCCCCTAA GATGTTACTC AGGCTCCCGG CAGCCGTGTC AACTCTTCAA 
GAAATGGCAC CAGGAACAAC ATTTAACCCG GTCATTGGTG ATTCATCTGT GGATCCAAAA 
AAGGTTAAGA CCCTCGTGTT CTGCTCCGGC AAACATTTCT ACTCCCTGGT GAAACAAAGA 
GAATCTCTGG GGGCCAAGAA GCATGACTTT GCCATCATCC GAGTAGAGGA ACTCTGCCCC 
TTCCCGTTGG ATTCTTTACA GCAAGAGATG AGCAAATACA AACATGTTAA AGATCATATT 
TGGAGTCAGG AGGAACCTCA GAACATGGGT CCGTGGTCGT TTGTTTCTCC AAGGTTTGAA 
AAGCAGCTGG CCTGCAAGCT CCGTCTGGTG GGCCGGCCCC CTTTGCCAGT ACCCGCTGTA 
GGAATTGGCA CAGTTCACTT GCACCAGCAT GAAGATATCC TCGCCAAGAC CTTCGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic basis of alpha-aminoadipic and alpha-ketoadipic aciduria.
Journal of inherited metabolic disease38(5)873-9(2015 Sep)
Hagen J,te Brinke H,Wanders RJ,Knegt AC,Oussoren E,Hoogeboom AJ,Ruijter GJ,Becker D,Schwab KO,Franke I,Duran M,Waterham HR,Sass JO,Houten SM

DHTKD1 is essential for mitochondrial biogenesis and function maintenance.
FEBS letters587(21)3587-92(2013 Nov)
Xu W,Zhu H,Gu M,Luo Q,Ding J,Yao Y,Chen F,Wang Z

DHTKD1 mutations cause 2-aminoadipic and 2-oxoadipic aciduria.
American journal of human genetics91(6)1082-7(2012 Dec)
Danhauser K,Sauer SW,Haack TB,Wieland T,Staufner C,Graf E,Zschocke J,Strom TM,Traub T,Okun JG,Meitinger T,Hoffmann GF,Prokisch H,K?lker S

A nonsense mutation in DHTKD1 causes Charcot-Marie-Tooth disease type 2 in a large Chinese pedigree.
American journal of human genetics91(6)1088-94(2012 Dec)
Xu WY,Gu MM,Sun LH,Guo WT,Zhu HB,Ma JF,Yuan WT,Kuang Y,Ji BJ,Wu XL,Chen Y,Zhang HX,Sun FT,Huang W,Huang L,Chen SD,Wang ZG

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

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DHTKD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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