DHTKD1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DHTKD1
Entrez Gene ID	517113
Full Name	dehydrogenase E1 and transketolase domain containing 1
General protein information	Preferred Names dehydrogenase E1 and transketolase domain containing 1 Names probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001205838.1	NP_001192767.1	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
NM_001205838.2	NP_001192767.2	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
XM_015473956.1	XP_015329442.1	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial isoform X1
XM_015473957.1	XP_015329443.1	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial isoform X2
XM_015473957.2	XP_015329443.1	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DHTKD1	XP_001135864.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000551	XP_310532.5
	LOC696445	XP_001085071.2
Mus musculus (house mouse)	Dhtkd1	NP_001074600.1
Bos taurus (cattle)	DHTKD1	NP_001192767.1
Rattus norvegicus (Norway rat)	Dhtkd1	NP_001020891.1
Danio rerio (zebrafish)	dhtkd1	NP_001008619.1
Homo sapiens (human)	DHTKD1	NP_061176.3
Canis lupus familiaris (dog)	DHTKD1	XP_535192.3
Gallus gallus (chicken)	DHTKD1	XP_004937543.1
Drosophila melanogaster (fruit fly)	CG1544	NP_651849.1
Caenorhabditis elegans (roundworm)	ZK836.2	NP_001256410.1
Xenopus tropicalis (tropical clawed frog)	dhtkd1	XP_002931921.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DHTKD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHTKD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41165	NM_001205838.1 Latest version!	Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa216369	NM_001205838.2 Latest version!	Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa92217	XM_015473956.1 Latest version!	Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa92218	XM_015473957.2 Latest version!	Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OBa92218	XM_015473957.1	Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DHTKD1 ( NM_001205838.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192767.1 Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), mRNA.

Price & Availability↑

CloneID	OBa41165
Clone ID Related Accession (Same CDS sequence)	NM_001205838.1
Accession Version	NM_001205838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03089586.1, AAFC03089584.1 and AAFC03090688.1. On Apr 20, 2011 this sequence version replaced XM_595276.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCCTGGG TCGCTGTGGC AGCAGCAAGA CTGGGCCTCG GCCGGGCCTC TCCGCTCTTC 
CTCCGGCGCG GCTACCAGAC AGAGAGGGGT GTCTATGGCT ACCGGCCGAG GAAGCCCGAG 
AGCCGGGAGC CCGAGGGCGG CCTGGCGCGC CCCCCAGTGG ACCATGGCCT TGCCAGGTTG 
GTGACGGTGT ATTGTGAGCA TGGGCATAAA GCTGCCAAAA TCAACCCACT CTTCACTGGT 
CAAGCCCTGC AGGAAAATGT GCCTGAAATC CAAGCCCTGG TGCAGACTCT GCAGGGACCC 
TTTAATACCA CAGGATTATT GAACATGGGG AAGGAGGAGG CCTCTCTTGA GGAAGTCGTT 
GCCTATCTCA ACCAGATCTA CTGTGGGCAG ATTTCTATCG AAACCTCTCA GCTTCAGAGC 
CAGGAGGAGA AAGACTGGTT TGCCAAGCGG TTTGAGGAAC TGAAGAAGGA GGCATTTACC 
ACAGAAGAGC GAAAACACCT GTCAAAATTA ATGCTAGAAT CTCAGGAGTT TGACCACTTT 
TTGGCCACCA AGTTTGCCAC AGTGAAGCGA TACGGAGGCG AAGGAGCAGA AAGCATGATG 
GGCTTTTTCT ACGAGCTGCT GAAAATGGCG GCTTACAGCG GAGTAACGGA TGTTATTATT 
GGGATGCCCC ATAGAGGGAG GCTGAACTTG CTGACGGGCC TTCTGCAGTT CCCTCCAGAG 
CTGATGTTCC GTAAAATGCG AGGCTTAAGT GAATTTCCAG AAAACATTTC AGCCGTTGGC 
GATGTCCTGT CTCACCTAAC GTCCTCGGTT GACCTGGACT TCGGTGCGCA CCATCCCCTC 
CATGTGACTA TGCTGCCCAA CCCCTCGCAC CTGGAAGCCG TGAACCCTGT GGCAGTCGGG 
AAGACTCGGG GCAGGCAGCA GTCCAGGCAG GATGGGGACT ACTCCCTGGA CAGTTCAGCT 
CAGCCTGGGG ACAAAGTCAT CTGCTTACAG GTTCATGGTG ATGCTTCTTT CTGTGGTCAA 
GGGATTGTTC CTGAAACATT TACGCTCTCT AATCTCCCGC ATTTCAGAAT TGGTGGGAGT 
GTCCATTTGA TTGTCAATAA CCAGCTGGGT TACACCACTC CTGCAGAAAG AGGAAGGTCT 
TCCTTATACT GTAGTGACAT CGGGAAGCTG GTGGGCTGCG CCATCATCCA CGTCAATGGA 
GACAGCCCAG AGGAAGTGGT GCGGGCCGCA CAACTGGCGT TCGAATACCA GCGCCAGTTC 
CGGAAAGATG TGATTGTTGA CTTGTTGTGC TACAGGCAGT GGGGCCACAA TGAGCTGGAC 
GAGCCCTTCT TCACCAACCC GGGCATGTAC CAGATCATCA GAGCCCGGAA GAGCATCCCT 
GATATGTATG CAGAGTACCT TATGGCCAAC GGACTCACAA CCCAGGAGGA GGTGTCGGAA 
ATCAAAGCCT CCTATTATGT GAAGTTAAAT GGCCATTTGA CTCATGCGGC CCACTACAGG 
CCCCCTGCCA CGAGCCTGCA GGCCCATTGG CAAGGCTTGG TTCAGCCGGA AGCTTGTATT 
ACTTCCTGGA ACACAGGTGT GCCCCTTGAC CTCCTGCAGT TTATTGGTAA GAAGTCTGTG 
GAGGTGCCTG AGGAGTTCCA GATACACAGT CACCTTCTGA AGATGCATGT GCAGTCTCGA 
GTGGAGAAAG TGATGGATGG AACAAAGTTA GACTGGGCCA CGGCAGAAGC TCTTGCATTG 
GGCTCATTGC TTGCTGAAGG TTTTAATGTC CGTCTGAGTG GCCAAGATGT GGGCCGTGGA 
ACTTTTAGCC AGAGGCACGC AATGGTTGTT TGCCAGAAGA CAGATGACAC TTATATCCCC 
CTGAACCATA TGGACCCTAA TCAAAAGGGG TTTCTAGAGG TCAGCAACAG CCCACTGTCG 
GAAGAGGCCG TCCTGGGATT TGAGTATGGG ATGAGCATTG AGAGCCCTAA CCTGCTGCCC 
CTCTGGGAGG CTCAGTTTGG CGATTTCTTC AATGGTGCCC AGATCATCTT TGACACGTTC 
ATCTCTGGAG GCGAGGCCAA GTGGCTCCTC CAGAGCGGCA TTGTGGTCCT CCTTCCCCAC 
GGGTACGACG GGGCTGGACC GGAGCACTCA TCTTGTCGAA TAGAGCGTTT CTTGCAGATG 
TGTGACAGCA CAGAGGAGGG TGTGGATGGG GACACTGTGA ACATGTGCGT GGCTCACCCC 
ACCACGCCCG CCCAGTACTT CCACCTGCTC CGGAGACAGA TGATTCGGAA CTTCAGGAAG 
CCCCTCATCC TTGTTTCTCC GAAGACACTG CTCAGACTCC CCGCAGCTGT ATCAACTCTT 
CAGGAAATGG CACCAGGAAC AACATTCAAA CCGGTCATTG GAGATTCATC TGTGGATCCA 
AAAAAGGTTA AGAGTCTGGT GTTCTGCTGT GGCAAACATT TCTATGCCCT GATGAAGCAA 
AGAGAATCTT TGGAGGCCAG GAAGCATGAC TTTGCCATCA TCCGAATAGA AGACCTCTGT 
CCTTTCCCAC TGGATTCGCT GCAGCAAGAG ATGAGCAAAT ACAAACACGT TAGAGATGTT 
ATCTGGAGTC AAGAGGAGCC TCAGAACATG GGTCCTTGGT TTTTTGTTTC TCCAAGGTTT 
GAGAAGCAGC TGGCCTGCAA GCTCCGTCTT GTGAGCCGAC CCGCGTTGCC TGTACCGGCT 
GTGGGAATCG GCACCATTCA CCTACAGCAG CATGAAGAAA TCCTCACTAA GACCTTCAAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192767.1
CDS	126..2891
Translation	MAWVAVAAARLGLGRASPLFLRRGYQTERGVYGYRPRKPESREPEGGLARPPVDHGLARLVTVYCEHGHKAAKINPLFTGQALQENVPEIQALVQTLQGPFNTTGLLNMGKEEASLEEVVAYLNQIYCGQISIETSQLQSQEEKDWFAKRFEELKKEAFTTEERKHLSKLMLESQEFDHFLATKFATVKRYGGEGAESMMGFFYELLKMAAYSGVTDVIIGMPHRGRLNLLTGLLQFPPELMFRKMRGLSEFPENISAVGDVLSHLTSSVDLDFGAHHPLHVTMLPNPSHLEAVNPVAVGKTRGRQQSRQDGDYSLDSSAQPGDKVICLQVHGDASFCGQGIVPETFTLSNLPHFRIGGSVHLIVNNQLGYTTPAERGRSSLYCSDIGKLVGCAIIHVNGDSPEEVVRAAQLAFEYQRQFRKDVIVDLLCYRQWGHNELDEPFFTNPGMYQIIRARKSIPDMYAEYLMANGLTTQEEVSEIKASYYVKLNGHLTHAAHYRPPATSLQAHWQGLVQPEACITSWNTGVPLDLLQFIGKKSVEVPEEFQIHSHLLKMHVQSRVEKVMDGTKLDWATAEALALGSLLAEGFNVRLSGQDVGRGTFSQRHAMVVCQKTDDTYIPLNHMDPNQKGFLEVSNSPLSEEAVLGFEYGMSIESPNLLPLWEAQFGDFFNGAQIIFDTFISGGEAKWLLQSGIVVLLPHGYDGAGPEHSSCRIERFLQMCDSTEEGVDGDTVNMCVAHPTTPAQYFHLLRRQMIRNFRKPLILVSPKTLLRLPAAVSTLQEMAPGTTFKPVIGDSSVDPKKVKSLVFCCGKHFYALMKQRESLEARKHDFAIIRIEDLCPFPLDSLQQEMSKYKHVRDVIWSQEEPQNMGPWFFVSPRFEKQLACKLRLVSRPALPVPAVGIGTIHLQQHEEILTKTFNP

Target ORF information:

RefSeq Version	NM_001205838.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205838.1

ORF Insert Sequence:

ATGGCCTGGG TCGCTGTGGC AGCAGCAAGA CTGGGCCTCG GCCGGGCCTC TCCGCTCTTC 
CTCCGGCGCG GCTACCAGAC AGAGAGGGGT GTCTATGGCT ACCGGCCGAG GAAGCCCGAG 
AGCCGGGAGC CCGAGGGCGG CCTGGCGCGC CCCCCAGTGG ACCATGGCCT TGCCAGGTTG 
GTGACGGTGT ATTGTGAGCA TGGGCATAAA GCTGCCAAAA TCAACCCACT CTTCACTGGT 
CAAGCCCTGC AGGAAAATGT GCCTGAAATC CAAGCCCTGG TGCAGACTCT GCAGGGACCC 
TTTAATACCA CAGGATTATT GAACATGGGG AAGGAGGAGG CCTCTCTTGA GGAAGTCGTT 
GCCTATCTCA ACCAGATCTA CTGTGGGCAG ATTTCTATCG AAACCTCTCA GCTTCAGAGC 
CAGGAGGAGA AAGACTGGTT TGCCAAGCGG TTTGAGGAAC TGAAGAAGGA GGCATTTACC 
ACAGAAGAGC GAAAACACCT GTCAAAATTA ATGCTAGAAT CTCAGGAGTT TGACCACTTT 
TTGGCCACCA AGTTTGCCAC AGTGAAGCGA TACGGAGGCG AAGGAGCAGA AAGCATGATG 
GGCTTTTTCT ACGAGCTGCT GAAAATGGCG GCTTACAGCG GAGTAACGGA TGTTATTATT 
GGGATGCCCC ATAGAGGGAG GCTGAACTTG CTGACGGGCC TTCTGCAGTT CCCTCCAGAG 
CTGATGTTCC GTAAAATGCG AGGCTTAAGT GAATTTCCAG AAAACATTTC AGCCGTTGGC 
GATGTCCTGT CTCACCTAAC GTCCTCGGTT GACCTGGACT TCGGTGCGCA CCATCCCCTC 
CATGTGACTA TGCTGCCCAA CCCCTCGCAC CTGGAAGCCG TGAACCCTGT GGCAGTCGGG 
AAGACTCGGG GCAGGCAGCA GTCCAGGCAG GATGGGGACT ACTCCCTGGA CAGTTCAGCT 
CAGCCTGGGG ACAAAGTCAT CTGCTTACAG GTTCATGGTG ATGCTTCTTT CTGTGGTCAA 
GGGATTGTTC CTGAAACATT TACGCTCTCT AATCTCCCGC ATTTCAGAAT TGGTGGGAGT 
GTCCATTTGA TTGTCAATAA CCAGCTGGGT TACACCACTC CTGCAGAAAG AGGAAGGTCT 
TCCTTATACT GTAGTGACAT CGGGAAGCTG GTGGGCTGCG CCATCATCCA CGTCAATGGA 
GACAGCCCAG AGGAAGTGGT GCGGGCCGCA CAACTGGCGT TCGAATACCA GCGCCAGTTC 
CGGAAAGATG TGATTGTTGA CTTGTTGTGC TACAGGCAGT GGGGCCACAA TGAGCTGGAC 
GAGCCCTTCT TCACCAACCC GGGCATGTAC CAGATCATCA GAGCCCGGAA GAGCATCCCT 
GATATGTATG CAGAGTACCT TATGGCCAAC GGACTCACAA CCCAGGAGGA GGTGTCGGAA 
ATCAAAGCCT CCTATTATGT GAAGTTAAAT GGCCATTTGA CTCATGCGGC CCACTACAGG 
CCCCCTGCCA CGAGCCTGCA GGCCCATTGG CAAGGCTTGG TTCAGCCGGA AGCTTGTATT 
ACTTCCTGGA ACACAGGTGT GCCCCTTGAC CTCCTGCAGT TTATTGGTAA GAAGTCTGTG 
GAGGTGCCTG AGGAGTTCCA GATACACAGT CACCTTCTGA AGATGCATGT GCAGTCTCGA 
GTGGAGAAAG TGATGGATGG AACAAAGTTA GACTGGGCCA CGGCAGAAGC TCTTGCATTG 
GGCTCATTGC TTGCTGAAGG TTTTAATGTC CGTCTGAGTG GCCAAGATGT GGGCCGTGGA 
ACTTTTAGCC AGAGGCACGC AATGGTTGTT TGCCAGAAGA CAGATGACAC TTATATCCCC 
CTGAACCATA TGGACCCTAA TCAAAAGGGG TTTCTAGAGG TCAGCAACAG CCCACTGTCG 
GAAGAGGCCG TCCTGGGATT TGAGTATGGG ATGAGCATTG AGAGCCCTAA CCTGCTGCCC 
CTCTGGGAGG CTCAGTTTGG CGATTTCTTC AATGGTGCCC AGATCATCTT TGACACGTTC 
ATCTCTGGAG GCGAGGCCAA GTGGCTCCTC CAGAGCGGCA TTGTGGTCCT CCTTCCCCAC 
GGGTACGACG GGGCTGGACC GGAGCACTCA TCTTGTCGAA TAGAGCGTTT CTTGCAGATG 
TGTGACAGCA CAGAGGAGGG TGTGGATGGG GACACTGTGA ACATGTGCGT GGCTCACCCC 
ACCACGCCCG CCCAGTACTT CCACCTGCTC CGGAGACAGA TGATTCGGAA CTTCAGGAAG 
CCCCTCATCC TTGTTTCTCC GAAGACACTG CTCAGACTCC CCGCAGCTGT ATCAACTCTT 
CAGGAAATGG CACCAGGAAC AACATTCAAA CCGGTCATTG GAGATTCATC TGTGGATCCA 
AAAAAGGTTA AGAGTCTGGT GTTCTGCTGT GGCAAACATT TCTATGCCCT GATGAAGCAA 
AGAGAATCTT TGGAGGCCAG GAAGCATGAC TTTGCCATCA TCCGAATAGA AGACCTCTGT 
CCTTTCCCAC TGGATTCGCT GCAGCAAGAG ATGAGCAAAT ACAAACACGT TAGAGATGTT 
ATCTGGAGTC AAGAGGAGCC TCAGAACATG GGTCCTTGGT TTTTTGTTTC TCCAAGGTTT 
GAGAAGCAGC TGGCCTGCAA GCTCCGTCTT GTGAGCCGAC CCGCGTTGCC TGTACCGGCT 
GTGGGAATCG GCACCATTCA CCTACAGCAG CATGAAGAAA TCCTCACTAA GACCTTCAAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHTKD1 ( NM_001205838.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192767.2 Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), mRNA.

Price & Availability↑

CloneID	OBa216369
Clone ID Related Accession (Same CDS sequence)	NM_001205838.2
Accession Version	NM_001205838.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000013.1. On Jun 30, 2017 this sequence version replaced NM_001205838.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5721695.154.1, SRR5571262.30323.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCCTGGG TCGCTGTGGC AGCAGCAAGA CTGGGCCTCG GCCGGGCCTC TCCGCTCTTC 
CTCCGGCGCG GCTACCAGAC AGAGAGGGGT GTCTATGGCT ACCGGCCGAG GAAGCCCGAG 
AGCCGGGAGC CCGAGGGCGG CCTGGCGCGC CCCCCAGTGG ACCATGGCCT TGCCAGGTTG 
GTGACGGTGT ATTGTGAGCA TGGGCATAAA GCTGCCAAAA TCAACCCACT CTTCACTGGT 
CAAGCCCTGC AGGAAAATGT GCCTGAAATC CAAGCCCTGG TGCAGACTCT GCAGGGACCC 
TTTAATACCA CAGGATTATT GAACATGGGG AAGGAGGAGG CCTCTCTTGA GGAAGTCGTT 
GCCTATCTCA ACCAGATCTA CTGTGGGCAG ATTTCTATCG AAACCTCTCA GCTTCAGAGC 
CAGGAGGAGA AAGACTGGTT TGCCAAGCGG TTTGAGGAAC TGAAGAAGGA GGCATTTACC 
ACAGAAGAGC GAAAACACCT GTCAAAATTA ATGCTAGAAT CTCAGGAGTT TGACCACTTT 
TTGGCCACCA AGTTTGCCAC AGTGAAGCGA TACGGAGGCG AAGGAGCAGA AAGCATGATG 
GGCTTTTTCT ACGAGCTGCT GAAAATGGCG GCTTACAGCG GAGTAACGGA TGTTATTATT 
GGGATGCCCC ATAGAGGGAG GCTGAACTTG CTGACGGGCC TTCTGCAGTT CCCTCCAGAG 
CTGATGTTCC GTAAAATGCG AGGCTTAAGT GAATTTCCAG AAAACATTTC AGCCGTTGGC 
GATGTCCTGT CTCACCTAAC GTCCTCGGTT GACCTGGACT TCGGTGCGCA CCATCCCCTC 
CATGTGACTA TGCTGCCCAA CCCCTCGCAC CTGGAAGCCG TGAACCCTGT GGCAGTCGGG 
AAGACTCGGG GCAGGCAGCA GTCCAGGCAG GATGGGGACT ACTCCCTGGA CAGTTCAGCT 
CAGCCTGGGG ACAAAGTCAT CTGCTTACAG GTTCATGGTG ATGCTTCTTT CTGTGGTCAA 
GGGATTGTTC CTGAAACATT TACGCTCTCT AATCTCCCGC ATTTCAGAAT TGGTGGGAGT 
GTCCATTTGA TTGTCAATAA CCAGCTGGGT TACACCACTC CTGCAGAAAG AGGAAGGTCT 
TCCTTATACT GTAGTGACAT CGGGAAGCTG GTGGGCTGCG CCATCATCCA CGTCAATGGA 
GACAGCCCAG AGGAAGTGGT GCGGGCCGCA CAACTGGCGT TCGAATACCA GCGCCAGTTC 
CGGAAAGATG TGATTGTTGA CTTGTTGTGC TACAGGCAGT GGGGCCACAA TGAGCTGGAC 
GAGCCCTTCT TCACCAACCC GGGCATGTAC CAGATCATCA GAGCCCGGAA GAGCATCCCT 
GATATGTATG CAGAGTACCT TATGGCCAAC GGACTCACAA CCCAGGAGGA GGTGTCGGAA 
ATCAAAGCCT CCTATTATGT GAAGTTAAAT GGCCATTTGA CTCATGCGGC CCACTACAGG 
CCCCCTGCCA CGAGCCTGCA GGCCCATTGG CAAGGCTTGG TTCAGCCGGA AGCTTGTATT 
ACTTCCTGGA ACACAGGTGT GCCCCTTGAC CTCCTGCAGT TTATTGGTAA GAAGTCTGTG 
GAGGTGCCTG AGGAGTTCCA GATACACAGT CACCTTCTGA AGATGCATGT GCAGTCTCGA 
GTGGAGAAAG TGATGGATGG AACAAAGTTA GACTGGGCCA CGGCAGAAGC TCTTGCATTG 
GGCTCATTGC TTGCTGAAGG TTTTAATGTC CGTCTGAGTG GCCAAGATGT GGGCCGTGGA 
ACTTTTAGCC AGAGGCACGC AATGGTTGTT TGCCAGAAGA CAGATGACAC TTATATCCCC 
CTGAACCATA TGGACCCTAA TCAAAAGGGG TTTCTAGAGG TCAGCAACAG CCCACTGTCG 
GAAGAGGCCG TCCTGGGATT TGAGTATGGG ATGAGCATTG AGAGCCCTAA CCTGCTGCCC 
CTCTGGGAGG CTCAGTTTGG CGATTTCTTC AATGGTGCCC AGATCATCTT TGACACGTTC 
ATCTCTGGAG GCGAGGCCAA GTGGCTCCTC CAGAGCGGCA TTGTGGTCCT CCTTCCCCAC 
GGGTACGACG GGGCTGGACC GGAGCACTCA TCTTGTCGAA TAGAGCGTTT CTTGCAGATG 
TGTGACAGCA CAGAGGAGGG TGTGGATGGG GACACTGTGA ACATGTGCGT GGCTCACCCC 
ACCACGCCCG CCCAGTACTT CCACCTGCTC CGGAGACAGA TGATTCGGAA CTTCAGGAAG 
CCCCTCATCC TTGTTTCTCC GAAGACACTG CTCAGACTCC CCGCAGCTGT ATCAACTCTT 
CAGGAAATGG CACCAGGAAC AACATTCAAA CCGGTCATTG GAGATTCATC TGTGGATCCA 
AAAAAGGTTA AGAGTCTGGT GTTCTGCTGT GGCAAACATT TCTATGCCCT GATGAAGCAA 
AGAGAATCTT TGGAGGCCAG GAAGCATGAC TTTGCCATCA TCCGAATAGA AGAACTCTGT 
CCTTTCCCAC TGGATTCGCT GCAGCAAGAG ATGAGCAAAT ACAAACACGT TAGAGATGTT 
ATCTGGAGTC AAGAGGAGCC TCAGAACATG GGTCCTTGGT TTTTTGTTTC TCCAAGGTTT 
GAGAAGCAGC TGGCCTGCAA GCTCCGTCTT GTGAGCCGAC CCGCGTTGCC TGTACCGGCT 
GTGGGAATCG GCACCATTCA CCTACAGCAG CATGAAGAAA TCCTCACTAA GACCTTCAAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192767.2
CDS	126..2891
Translation	MAWVAVAAARLGLGRASPLFLRRGYQTERGVYGYRPRKPESREPEGGLARPPVDHGLARLVTVYCEHGHKAAKINPLFTGQALQENVPEIQALVQTLQGPFNTTGLLNMGKEEASLEEVVAYLNQIYCGQISIETSQLQSQEEKDWFAKRFEELKKEAFTTEERKHLSKLMLESQEFDHFLATKFATVKRYGGEGAESMMGFFYELLKMAAYSGVTDVIIGMPHRGRLNLLTGLLQFPPELMFRKMRGLSEFPENISAVGDVLSHLTSSVDLDFGAHHPLHVTMLPNPSHLEAVNPVAVGKTRGRQQSRQDGDYSLDSSAQPGDKVICLQVHGDASFCGQGIVPETFTLSNLPHFRIGGSVHLIVNNQLGYTTPAERGRSSLYCSDIGKLVGCAIIHVNGDSPEEVVRAAQLAFEYQRQFRKDVIVDLLCYRQWGHNELDEPFFTNPGMYQIIRARKSIPDMYAEYLMANGLTTQEEVSEIKASYYVKLNGHLTHAAHYRPPATSLQAHWQGLVQPEACITSWNTGVPLDLLQFIGKKSVEVPEEFQIHSHLLKMHVQSRVEKVMDGTKLDWATAEALALGSLLAEGFNVRLSGQDVGRGTFSQRHAMVVCQKTDDTYIPLNHMDPNQKGFLEVSNSPLSEEAVLGFEYGMSIESPNLLPLWEAQFGDFFNGAQIIFDTFISGGEAKWLLQSGIVVLLPHGYDGAGPEHSSCRIERFLQMCDSTEEGVDGDTVNMCVAHPTTPAQYFHLLRRQMIRNFRKPLILVSPKTLLRLPAAVSTLQEMAPGTTFKPVIGDSSVDPKKVKSLVFCCGKHFYALMKQRESLEARKHDFAIIRIEELCPFPLDSLQQEMSKYKHVRDVIWSQEEPQNMGPWFFVSPRFEKQLACKLRLVSRPALPVPAVGIGTIHLQQHEEILTKTFNP

Target ORF information:

RefSeq Version	NM_001205838.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205838.2

ORF Insert Sequence:

ATGGCCTGGG TCGCTGTGGC AGCAGCAAGA CTGGGCCTCG GCCGGGCCTC TCCGCTCTTC 
CTCCGGCGCG GCTACCAGAC AGAGAGGGGT GTCTATGGCT ACCGGCCGAG GAAGCCCGAG 
AGCCGGGAGC CCGAGGGCGG CCTGGCGCGC CCCCCAGTGG ACCATGGCCT TGCCAGGTTG 
GTGACGGTGT ATTGTGAGCA TGGGCATAAA GCTGCCAAAA TCAACCCACT CTTCACTGGT 
CAAGCCCTGC AGGAAAATGT GCCTGAAATC CAAGCCCTGG TGCAGACTCT GCAGGGACCC 
TTTAATACCA CAGGATTATT GAACATGGGG AAGGAGGAGG CCTCTCTTGA GGAAGTCGTT 
GCCTATCTCA ACCAGATCTA CTGTGGGCAG ATTTCTATCG AAACCTCTCA GCTTCAGAGC 
CAGGAGGAGA AAGACTGGTT TGCCAAGCGG TTTGAGGAAC TGAAGAAGGA GGCATTTACC 
ACAGAAGAGC GAAAACACCT GTCAAAATTA ATGCTAGAAT CTCAGGAGTT TGACCACTTT 
TTGGCCACCA AGTTTGCCAC AGTGAAGCGA TACGGAGGCG AAGGAGCAGA AAGCATGATG 
GGCTTTTTCT ACGAGCTGCT GAAAATGGCG GCTTACAGCG GAGTAACGGA TGTTATTATT 
GGGATGCCCC ATAGAGGGAG GCTGAACTTG CTGACGGGCC TTCTGCAGTT CCCTCCAGAG 
CTGATGTTCC GTAAAATGCG AGGCTTAAGT GAATTTCCAG AAAACATTTC AGCCGTTGGC 
GATGTCCTGT CTCACCTAAC GTCCTCGGTT GACCTGGACT TCGGTGCGCA CCATCCCCTC 
CATGTGACTA TGCTGCCCAA CCCCTCGCAC CTGGAAGCCG TGAACCCTGT GGCAGTCGGG 
AAGACTCGGG GCAGGCAGCA GTCCAGGCAG GATGGGGACT ACTCCCTGGA CAGTTCAGCT 
CAGCCTGGGG ACAAAGTCAT CTGCTTACAG GTTCATGGTG ATGCTTCTTT CTGTGGTCAA 
GGGATTGTTC CTGAAACATT TACGCTCTCT AATCTCCCGC ATTTCAGAAT TGGTGGGAGT 
GTCCATTTGA TTGTCAATAA CCAGCTGGGT TACACCACTC CTGCAGAAAG AGGAAGGTCT 
TCCTTATACT GTAGTGACAT CGGGAAGCTG GTGGGCTGCG CCATCATCCA CGTCAATGGA 
GACAGCCCAG AGGAAGTGGT GCGGGCCGCA CAACTGGCGT TCGAATACCA GCGCCAGTTC 
CGGAAAGATG TGATTGTTGA CTTGTTGTGC TACAGGCAGT GGGGCCACAA TGAGCTGGAC 
GAGCCCTTCT TCACCAACCC GGGCATGTAC CAGATCATCA GAGCCCGGAA GAGCATCCCT 
GATATGTATG CAGAGTACCT TATGGCCAAC GGACTCACAA CCCAGGAGGA GGTGTCGGAA 
ATCAAAGCCT CCTATTATGT GAAGTTAAAT GGCCATTTGA CTCATGCGGC CCACTACAGG 
CCCCCTGCCA CGAGCCTGCA GGCCCATTGG CAAGGCTTGG TTCAGCCGGA AGCTTGTATT 
ACTTCCTGGA ACACAGGTGT GCCCCTTGAC CTCCTGCAGT TTATTGGTAA GAAGTCTGTG 
GAGGTGCCTG AGGAGTTCCA GATACACAGT CACCTTCTGA AGATGCATGT GCAGTCTCGA 
GTGGAGAAAG TGATGGATGG AACAAAGTTA GACTGGGCCA CGGCAGAAGC TCTTGCATTG 
GGCTCATTGC TTGCTGAAGG TTTTAATGTC CGTCTGAGTG GCCAAGATGT GGGCCGTGGA 
ACTTTTAGCC AGAGGCACGC AATGGTTGTT TGCCAGAAGA CAGATGACAC TTATATCCCC 
CTGAACCATA TGGACCCTAA TCAAAAGGGG TTTCTAGAGG TCAGCAACAG CCCACTGTCG 
GAAGAGGCCG TCCTGGGATT TGAGTATGGG ATGAGCATTG AGAGCCCTAA CCTGCTGCCC 
CTCTGGGAGG CTCAGTTTGG CGATTTCTTC AATGGTGCCC AGATCATCTT TGACACGTTC 
ATCTCTGGAG GCGAGGCCAA GTGGCTCCTC CAGAGCGGCA TTGTGGTCCT CCTTCCCCAC 
GGGTACGACG GGGCTGGACC GGAGCACTCA TCTTGTCGAA TAGAGCGTTT CTTGCAGATG 
TGTGACAGCA CAGAGGAGGG TGTGGATGGG GACACTGTGA ACATGTGCGT GGCTCACCCC 
ACCACGCCCG CCCAGTACTT CCACCTGCTC CGGAGACAGA TGATTCGGAA CTTCAGGAAG 
CCCCTCATCC TTGTTTCTCC GAAGACACTG CTCAGACTCC CCGCAGCTGT ATCAACTCTT 
CAGGAAATGG CACCAGGAAC AACATTCAAA CCGGTCATTG GAGATTCATC TGTGGATCCA 
AAAAAGGTTA AGAGTCTGGT GTTCTGCTGT GGCAAACATT TCTATGCCCT GATGAAGCAA 
AGAGAATCTT TGGAGGCCAG GAAGCATGAC TTTGCCATCA TCCGAATAGA AGAACTCTGT 
CCTTTCCCAC TGGATTCGCT GCAGCAAGAG ATGAGCAAAT ACAAACACGT TAGAGATGTT 
ATCTGGAGTC AAGAGGAGCC TCAGAACATG GGTCCTTGGT TTTTTGTTTC TCCAAGGTTT 
GAGAAGCAGC TGGCCTGCAA GCTCCGTCTT GTGAGCCGAC CCGCGTTGCC TGTACCGGCT 
GTGGGAATCG GCACCATTCA CCTACAGCAG CATGAAGAAA TCCTCACTAA GACCTTCAAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHTKD1 ( XM_015473956.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329442.1 Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa92217
Clone ID Related Accession (Same CDS sequence)	XM_015473956.1
Accession Version	XM_015473956.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104362.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGGG TCGCTGTGGC AGCAGCAAGA CTGGGCCTCG GCCGGGCCTC TCCGCTCTTC 
CTCCGGCGCG GCTACCAGAC AGAGAGGGGT GTCTATGGCT ACCGGCCGAG GAAGCCCGAG 
AGCCGGGAGC CCGAGGGCGG CCTGGCGCGC CCCCCAGTGG ACCATGGCCT TGCCAGGTTG 
GTGACGGTGT ATTGTGAGCA TGGGCATAAA GCTGCCAAAA TCAACCCACT CTTCACTGGT 
CAAGCCCTGC AGGAAAATGT GCCTGAAATC CAAGCCCTGG TGCAGACTCT GCAGGGACCC 
TTTAATACCA CAGGATTATT GAACATGGGG AAGGAGGAGG CCTCTCTTGA GGAAGTCGTT 
GCCTATCTCA ACCAGATCTA CTGTGGGCAG ATTTCTATCG AAACCTCTCA GCTTCAGAGC 
CAGGAGGAGA AAGACTGGTT TGCCAAGCGG TTTGAGGAAC TGAAGAAGGA GGCATTTACC 
ACAGAAGAGC GAAAACACCT GTCAAAATTA ATGCTAGAAT CTCAGGAGTT TGACCACTTT 
TTGGCCACCA AGTTTGCCAC AGTGAAGCGA TACGGAGGCG AAGGAGCAGA AAGCATGATG 
GGCTTTTTCT ACGAGCTGCT GAAAATGGCG GCTTACAGCG GAGTAACGGA TGTTATTATT 
GGGATGCCCC ATAGAGGGAG GCTGAACTTG CTGACGGGCC TTCTGCAGTT CCCTCCAGAG 
CTGATGTTCC GTAAAATGCG AGGCTTAAGT GAATTTCCAG AAAACATTTC AGCCGTTGGC 
GATGTCCTGT CTCACCTAAC GTCCTCGGTT GACCTGGACT TCGGTGCGCA CCATCCCCTC 
CATGTGACTA TGCTGCCCAA CCCCTCGCAC CTGGAAGCCG TGAACCCTGT GGCAGTCGGG 
AAGACTCGGG GCAGGCAGCA GTCCAGGCAG GATGGGGACT ACTCCCTGGA CAGTTCAGCT 
CAGCCTGGGG ACAAAGTCAT CTGCTTACAG GTTCATGGTG ATGCTTCTTT CTGTGGTCAA 
GGGATTGTTC CTGAAACATT TACGCTCTCT AATCTCCCGC ATTTCAGAAT TGGTGGGAGT 
GTCCATTTGA TTGTCAATAA CCAGCTGGGT TACACCACTC CTGCAGAAAG AGGAAGGTCT 
TCCTTATACT GTAGTGACAT CGGGAAGCTG GTGGGCTGCG CCATCATCCA CGTCAATGGA 
GACAGCCCAG AGGAAGTGGT GCGGGCCGCA CAACTGGCGT TCGAATACCA GCGCCAGTTC 
CGGAAAGATG TGATTGTTGA CTTGTTGTGC TACAGGCAGT GGGGCCACAA TGAGCTGGAC 
GAGCCCTTCT TCACCAACCC GGGCATGTAC CAGATCATCA GAGCCCGGAA GAGCATCCCT 
GATATGTATG CAGAGTACCT TATGGCCAAC GGACTCACAA CCCAGGAGGA GGTGTCGGAA 
ATCAAAGCCT CCTATTATGT GAAGTTAAAT GGCCATTTGA CTCATGCGGC CCACTACAGG 
CCCCCTGCCA CGAGCCTGCA GGCCCATTGG CAAGGCTTGG TTCAGCCGGA AGCTTGTATT 
ACTTCCTGGA ACACAGGTGT GCCCCTTGAC CTCCTGCAGT TTATTGGTAA GAAGTCTGTG 
GAGGTGCCTG AGGAGTTCCA GATACACAGT CACCTTCTGA AGATGCATGT GCAGTCTCGA 
GTGGAGAAAG TGATGGATGG AACAAAGTTA GACTGGGCCA CGGCAGAAGC TCTTGCATTG 
GGCTCATTGC TTGCTGAAGG TTTTAATGTC CGTCTGAGTG GCCAAGATGT GGGCCGTGGA 
ACTTTTAGCC AGAGGCACGC AATGGTTGTT TGCCAGAAGA CAGATGACAC TTATATCCCC 
CTGAACCATA TGGACCCTAA TCAAAAGGGG TTTCTAGAGG TCAGCAACAG CCCACTGTCG 
GAAGAGGCCG TCCTGGGATT TGAGTATGGG ATGAGCATTG AGAGCCCTAA CCTGCTGCCC 
CTCTGGGAGG CTCAGTTTGG CGATTTCTTC AATGGTGCCC AGATCATCTT TGACACGTTC 
ATCTCTGGAG GCGAGGCCAA GTGGCTCCTC CAGAGCGGCA TTGTGGTCCT CCTTCCCCAC 
GGGTACGACG GGGCTGGACC GGAGCACTCA TCTTGTCGAA TAGAGCGTTT CTTGCAGGCA 
GCTGTATCAA CTCTTCAGGA AATGGCACCA GGAACAACAT TCAAACCGGT CATTGGAGAT 
TCATCTGTGG ATCCAAAAAA GGTTAAGAGT CTGGTGTTCT GCTGTGGCAA ACATTTCTAT 
GCCCTGATGA AGCAAAGAGA ATCTTTGGAG GCCAGGAAGC ATGACTTTGC CATCATCCGA 
ATAGAAGAAC TCTGTCCTTT CCCACTGGAT TCGCTGCAGC AAGAGATGAG CAAATACAAA 
CACGTTAGAG ATGTTATCTG GAGTCAAGAG GAGCCTCAGA ACATGGGTCC TTGGTTTTTT 
GTTTCTCCAA GGTTTGAGAA GCAGCTGGCC TGCAAGCTCC GTCTTGTGAG CCGACCCGCG 
TTGCCTGTAC CGGCTGTGGG AATCGGCACC ATTCACCTAC AGCAGCATGA AGAAATCCTC 
ACTAAGACCT TCAACCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329442.1
CDS	127..2727
Translation	MAWVAVAAARLGLGRASPLFLRRGYQTERGVYGYRPRKPESREPEGGLARPPVDHGLARLVTVYCEHGHKAAKINPLFTGQALQENVPEIQALVQTLQGPFNTTGLLNMGKEEASLEEVVAYLNQIYCGQISIETSQLQSQEEKDWFAKRFEELKKEAFTTEERKHLSKLMLESQEFDHFLATKFATVKRYGGEGAESMMGFFYELLKMAAYSGVTDVIIGMPHRGRLNLLTGLLQFPPELMFRKMRGLSEFPENISAVGDVLSHLTSSVDLDFGAHHPLHVTMLPNPSHLEAVNPVAVGKTRGRQQSRQDGDYSLDSSAQPGDKVICLQVHGDASFCGQGIVPETFTLSNLPHFRIGGSVHLIVNNQLGYTTPAERGRSSLYCSDIGKLVGCAIIHVNGDSPEEVVRAAQLAFEYQRQFRKDVIVDLLCYRQWGHNELDEPFFTNPGMYQIIRARKSIPDMYAEYLMANGLTTQEEVSEIKASYYVKLNGHLTHAAHYRPPATSLQAHWQGLVQPEACITSWNTGVPLDLLQFIGKKSVEVPEEFQIHSHLLKMHVQSRVEKVMDGTKLDWATAEALALGSLLAEGFNVRLSGQDVGRGTFSQRHAMVVCQKTDDTYIPLNHMDPNQKGFLEVSNSPLSEEAVLGFEYGMSIESPNLLPLWEAQFGDFFNGAQIIFDTFISGGEAKWLLQSGIVVLLPHGYDGAGPEHSSCRIERFLQAAVSTLQEMAPGTTFKPVIGDSSVDPKKVKSLVFCCGKHFYALMKQRESLEARKHDFAIIRIEELCPFPLDSLQQEMSKYKHVRDVIWSQEEPQNMGPWFFVSPRFEKQLACKLRLVSRPALPVPAVGIGTIHLQQHEEILTKTFNP

Target ORF information:

RefSeq Version	XM_015473956.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473956.1

ORF Insert Sequence:

ATGGCCTGGG TCGCTGTGGC AGCAGCAAGA CTGGGCCTCG GCCGGGCCTC TCCGCTCTTC 
CTCCGGCGCG GCTACCAGAC AGAGAGGGGT GTCTATGGCT ACCGGCCGAG GAAGCCCGAG 
AGCCGGGAGC CCGAGGGCGG CCTGGCGCGC CCCCCAGTGG ACCATGGCCT TGCCAGGTTG 
GTGACGGTGT ATTGTGAGCA TGGGCATAAA GCTGCCAAAA TCAACCCACT CTTCACTGGT 
CAAGCCCTGC AGGAAAATGT GCCTGAAATC CAAGCCCTGG TGCAGACTCT GCAGGGACCC 
TTTAATACCA CAGGATTATT GAACATGGGG AAGGAGGAGG CCTCTCTTGA GGAAGTCGTT 
GCCTATCTCA ACCAGATCTA CTGTGGGCAG ATTTCTATCG AAACCTCTCA GCTTCAGAGC 
CAGGAGGAGA AAGACTGGTT TGCCAAGCGG TTTGAGGAAC TGAAGAAGGA GGCATTTACC 
ACAGAAGAGC GAAAACACCT GTCAAAATTA ATGCTAGAAT CTCAGGAGTT TGACCACTTT 
TTGGCCACCA AGTTTGCCAC AGTGAAGCGA TACGGAGGCG AAGGAGCAGA AAGCATGATG 
GGCTTTTTCT ACGAGCTGCT GAAAATGGCG GCTTACAGCG GAGTAACGGA TGTTATTATT 
GGGATGCCCC ATAGAGGGAG GCTGAACTTG CTGACGGGCC TTCTGCAGTT CCCTCCAGAG 
CTGATGTTCC GTAAAATGCG AGGCTTAAGT GAATTTCCAG AAAACATTTC AGCCGTTGGC 
GATGTCCTGT CTCACCTAAC GTCCTCGGTT GACCTGGACT TCGGTGCGCA CCATCCCCTC 
CATGTGACTA TGCTGCCCAA CCCCTCGCAC CTGGAAGCCG TGAACCCTGT GGCAGTCGGG 
AAGACTCGGG GCAGGCAGCA GTCCAGGCAG GATGGGGACT ACTCCCTGGA CAGTTCAGCT 
CAGCCTGGGG ACAAAGTCAT CTGCTTACAG GTTCATGGTG ATGCTTCTTT CTGTGGTCAA 
GGGATTGTTC CTGAAACATT TACGCTCTCT AATCTCCCGC ATTTCAGAAT TGGTGGGAGT 
GTCCATTTGA TTGTCAATAA CCAGCTGGGT TACACCACTC CTGCAGAAAG AGGAAGGTCT 
TCCTTATACT GTAGTGACAT CGGGAAGCTG GTGGGCTGCG CCATCATCCA CGTCAATGGA 
GACAGCCCAG AGGAAGTGGT GCGGGCCGCA CAACTGGCGT TCGAATACCA GCGCCAGTTC 
CGGAAAGATG TGATTGTTGA CTTGTTGTGC TACAGGCAGT GGGGCCACAA TGAGCTGGAC 
GAGCCCTTCT TCACCAACCC GGGCATGTAC CAGATCATCA GAGCCCGGAA GAGCATCCCT 
GATATGTATG CAGAGTACCT TATGGCCAAC GGACTCACAA CCCAGGAGGA GGTGTCGGAA 
ATCAAAGCCT CCTATTATGT GAAGTTAAAT GGCCATTTGA CTCATGCGGC CCACTACAGG 
CCCCCTGCCA CGAGCCTGCA GGCCCATTGG CAAGGCTTGG TTCAGCCGGA AGCTTGTATT 
ACTTCCTGGA ACACAGGTGT GCCCCTTGAC CTCCTGCAGT TTATTGGTAA GAAGTCTGTG 
GAGGTGCCTG AGGAGTTCCA GATACACAGT CACCTTCTGA AGATGCATGT GCAGTCTCGA 
GTGGAGAAAG TGATGGATGG AACAAAGTTA GACTGGGCCA CGGCAGAAGC TCTTGCATTG 
GGCTCATTGC TTGCTGAAGG TTTTAATGTC CGTCTGAGTG GCCAAGATGT GGGCCGTGGA 
ACTTTTAGCC AGAGGCACGC AATGGTTGTT TGCCAGAAGA CAGATGACAC TTATATCCCC 
CTGAACCATA TGGACCCTAA TCAAAAGGGG TTTCTAGAGG TCAGCAACAG CCCACTGTCG 
GAAGAGGCCG TCCTGGGATT TGAGTATGGG ATGAGCATTG AGAGCCCTAA CCTGCTGCCC 
CTCTGGGAGG CTCAGTTTGG CGATTTCTTC AATGGTGCCC AGATCATCTT TGACACGTTC 
ATCTCTGGAG GCGAGGCCAA GTGGCTCCTC CAGAGCGGCA TTGTGGTCCT CCTTCCCCAC 
GGGTACGACG GGGCTGGACC GGAGCACTCA TCTTGTCGAA TAGAGCGTTT CTTGCAGGCA 
GCTGTATCAA CTCTTCAGGA AATGGCACCA GGAACAACAT TCAAACCGGT CATTGGAGAT 
TCATCTGTGG ATCCAAAAAA GGTTAAGAGT CTGGTGTTCT GCTGTGGCAA ACATTTCTAT 
GCCCTGATGA AGCAAAGAGA ATCTTTGGAG GCCAGGAAGC ATGACTTTGC CATCATCCGA 
ATAGAAGAAC TCTGTCCTTT CCCACTGGAT TCGCTGCAGC AAGAGATGAG CAAATACAAA 
CACGTTAGAG ATGTTATCTG GAGTCAAGAG GAGCCTCAGA ACATGGGTCC TTGGTTTTTT 
GTTTCTCCAA GGTTTGAGAA GCAGCTGGCC TGCAAGCTCC GTCTTGTGAG CCGACCCGCG 
TTGCCTGTAC CGGCTGTGGG AATCGGCACC ATTCACCTAC AGCAGCATGA AGAAATCCTC 
ACTAAGACCT TCAACCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHTKD1 ( XM_015473957.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329443.1 Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa92218
Clone ID Related Accession (Same CDS sequence)	XM_015473957.1 , XM_015473957.2
Accession Version	XM_015473957.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104362.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGGG TCGCTGTGGC AGCAGCAAGA CTGGGCCTCG GCCGGGCCTC TCCGCTCTTC 
CTCCGGCGCG GCTACCAGAC AGAGAGGGGT GTCTATGGCT ACCGGCCGAG GAAGCCCGAG 
AGCCGGGAGC CCGAGGGCGG CCTGGCGCGC CCCCCAGTGG ACCATGGCCT TGCCAGGTTG 
GTGACGGTGT ATTGTGAGCA TGGGCATAAA GCTGCCAAAA TCAACCCACT CTTCACTGGT 
CAAGCCCTGC AGGAAAATGT GCCTGAAATC CAAGCCCTGG TGCAGACTCT GCAGGGACCC 
TTTAATACCA CAGGATTATT GAACATGGGG AAGGAGGAGG CCTCTCTTGA GGAAGTCGTT 
GCCTATCTCA ACCAGATCTA CTGTGGGCAG ATTTCTATCG AAACCTCTCA GCTTCAGAGC 
CAGGAGGAGA AAGACTGGTT TGCCAAGCGG TTTGAGGAAC TGAAGAAGGA GGCATTTACC 
ACAGAAGAGC GAAAACACCT GTCAAAATTA ATGCTAGAAT CTCAGGAGTT TGACCACTTT 
TTGGCCACCA AGTTTGCCAC AGTGAAGCGA TACGGAGGCG AAGGAGCAGA AAGCATGATG 
GGCTTTTTCT ACGAGCTGCT GAAAATGGCG GCTTACAGCG GAGTAACGGA TGTTATTATT 
GGGATGCCCC ATAGAGGGAG GCTGAACTTG CTGACGGGCC TTCTGCAGTT CCCTCCAGAG 
GTTCATGGTG ATGCTTCTTT CTGTGGTCAA GGGATTGTTC CTGAAACATT TACGCTCTCT 
AATCTCCCGC ATTTCAGAAT TGGTGGGAGT GTCCATTTGA TTGTCAATAA CCAGCTGGGT 
TACACCACTC CTGCAGAAAG AGGAAGGTCT TCCTTATACT GTAGTGACAT CGGGAAGCTG 
GTGGGCTGCG CCATCATCCA CGTCAATGGA GACAGCCCAG AGGAAGTGGT GCGGGCCGCA 
CAACTGGCGT TCGAATACCA GCGCCAGTTC CGGAAAGATG TGATTGTTGA CTTGTTGTGC 
TACAGGCAGT GGGGCCACAA TGAGCTGGAC GAGCCCTTCT TCACCAACCC GGGCATGTAC 
CAGATCATCA GAGCCCGGAA GAGCATCCCT GATATGTATG CAGAGTACCT TATGGCCAAC 
GGACTCACAA CCCAGGAGGA GGTGTCGGAA ATCAAAGCCT CCTATTATGT GAAGTTAAAT 
GGCCATTTGA CTCATGCGGC CCACTACAGG CCCCCTGCCA CGAGCCTGCA GGCCCATTGG 
CAAGGCTTGG TTCAGCCGGA AGCTTGTATT ACTTCCTGGA ACACAGGTGT GCCCCTTGAC 
CTCCTGCAGT TTATTGGTAA GAAGTCTGTG GAGGTGCCTG AGGAGTTCCA GATACACAGT 
CACCTTCTGA AGATGCATGT GCAGTCTCGA GTGGAGAAAG TGATGGATGG AACAAAGTTA 
GACTGGGCCA CGGCAGAAGC TCTTGCATTG GGCTCATTGC TTGCTGAAGG TTTTAATGTC 
CGTCTGAGTG GCCAAGATGT GGGCCGTGGA ACTTTTAGCC AGAGGCACGC AATGGTTGTT 
TGCCAGAAGA CAGATGACAC TTATATCCCC CTGAACCATA TGGACCCTAA TCAAAAGGGG 
TTTCTAGAGG TCAGCAACAG CCCACTGTCG GAAGAGGCCG TCCTGGGATT TGAGTATGGG 
ATGAGCATTG AGAGCCCTAA CCTGCTGCCC CTCTGGGAGG CTCAGTTTGG CGATTTCTTC 
AATGGTGCCC AGATCATCTT TGACACGTTC ATCTCTGGAG GCGAGGCCAA GTGGCTCCTC 
CAGAGCGGCA TTGTGGTCCT CCTTCCCCAC GGGTACGACG GGGCTGGACC GGAGCACTCA 
TCTTGTCGAA TAGAGCGTTT CTTGCAGATG TGTGACAGCA CAGAGGAGGG TGTGGATGGG 
GACACTGTGA ACATGTGCGT GGCTCACCCC ACCACGCCCG CCCAGTACTT CCACCTGCTC 
CGGAGACAGA TGATTCGGAA CTTCAGGAAG CCCCTCATCC TTGTTTCTCC GAAGACACTG 
CTCAGACTCC CCGCAGCTGT ATCAACTCTT CAGGAAATGG CACCAGGAAC AACATTCAAA 
CCGGTCATTG GAGATTCATC TGTGGATCCA AAAAAGGTTA AGAGTCTGGT GTTCTGCTGT 
GGCAAACATT TCTATGCCCT GATGAAGCAA AGAGAATCTT TGGAGGCCAG GAAGCATGAC 
TTTGCCATCA TCCGAATAGA AGAACTCTGT CCTTTCCCAC TGGATTCGCT GCAGCAAGAG 
ATGAGCAAAT ACAAACACGT TAGAGATGTT ATCTGGAGTC AAGAGGAGCC TCAGAACATG 
GGTCCTTGGT TTTTTGTTTC TCCAAGGTTT GAGAAGCAGC TGGCCTGCAA GCTCCGTCTT 
GTGAGCCGAC CCGCGTTGCC TGTACCGGCT GTGGGAATCG GCACCATTCA CCTACAGCAG 
CATGAAGAAA TCCTCACTAA GACCTTCAAC CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329443.1
CDS	127..2622
Translation	MAWVAVAAARLGLGRASPLFLRRGYQTERGVYGYRPRKPESREPEGGLARPPVDHGLARLVTVYCEHGHKAAKINPLFTGQALQENVPEIQALVQTLQGPFNTTGLLNMGKEEASLEEVVAYLNQIYCGQISIETSQLQSQEEKDWFAKRFEELKKEAFTTEERKHLSKLMLESQEFDHFLATKFATVKRYGGEGAESMMGFFYELLKMAAYSGVTDVIIGMPHRGRLNLLTGLLQFPPEVHGDASFCGQGIVPETFTLSNLPHFRIGGSVHLIVNNQLGYTTPAERGRSSLYCSDIGKLVGCAIIHVNGDSPEEVVRAAQLAFEYQRQFRKDVIVDLLCYRQWGHNELDEPFFTNPGMYQIIRARKSIPDMYAEYLMANGLTTQEEVSEIKASYYVKLNGHLTHAAHYRPPATSLQAHWQGLVQPEACITSWNTGVPLDLLQFIGKKSVEVPEEFQIHSHLLKMHVQSRVEKVMDGTKLDWATAEALALGSLLAEGFNVRLSGQDVGRGTFSQRHAMVVCQKTDDTYIPLNHMDPNQKGFLEVSNSPLSEEAVLGFEYGMSIESPNLLPLWEAQFGDFFNGAQIIFDTFISGGEAKWLLQSGIVVLLPHGYDGAGPEHSSCRIERFLQMCDSTEEGVDGDTVNMCVAHPTTPAQYFHLLRRQMIRNFRKPLILVSPKTLLRLPAAVSTLQEMAPGTTFKPVIGDSSVDPKKVKSLVFCCGKHFYALMKQRESLEARKHDFAIIRIEELCPFPLDSLQQEMSKYKHVRDVIWSQEEPQNMGPWFFVSPRFEKQLACKLRLVSRPALPVPAVGIGTIHLQQHEEILTKTFNP

Target ORF information:

RefSeq Version	XM_015473957.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473957.1

ORF Insert Sequence:

ATGGCCTGGG TCGCTGTGGC AGCAGCAAGA CTGGGCCTCG GCCGGGCCTC TCCGCTCTTC 
CTCCGGCGCG GCTACCAGAC AGAGAGGGGT GTCTATGGCT ACCGGCCGAG GAAGCCCGAG 
AGCCGGGAGC CCGAGGGCGG CCTGGCGCGC CCCCCAGTGG ACCATGGCCT TGCCAGGTTG 
GTGACGGTGT ATTGTGAGCA TGGGCATAAA GCTGCCAAAA TCAACCCACT CTTCACTGGT 
CAAGCCCTGC AGGAAAATGT GCCTGAAATC CAAGCCCTGG TGCAGACTCT GCAGGGACCC 
TTTAATACCA CAGGATTATT GAACATGGGG AAGGAGGAGG CCTCTCTTGA GGAAGTCGTT 
GCCTATCTCA ACCAGATCTA CTGTGGGCAG ATTTCTATCG AAACCTCTCA GCTTCAGAGC 
CAGGAGGAGA AAGACTGGTT TGCCAAGCGG TTTGAGGAAC TGAAGAAGGA GGCATTTACC 
ACAGAAGAGC GAAAACACCT GTCAAAATTA ATGCTAGAAT CTCAGGAGTT TGACCACTTT 
TTGGCCACCA AGTTTGCCAC AGTGAAGCGA TACGGAGGCG AAGGAGCAGA AAGCATGATG 
GGCTTTTTCT ACGAGCTGCT GAAAATGGCG GCTTACAGCG GAGTAACGGA TGTTATTATT 
GGGATGCCCC ATAGAGGGAG GCTGAACTTG CTGACGGGCC TTCTGCAGTT CCCTCCAGAG 
GTTCATGGTG ATGCTTCTTT CTGTGGTCAA GGGATTGTTC CTGAAACATT TACGCTCTCT 
AATCTCCCGC ATTTCAGAAT TGGTGGGAGT GTCCATTTGA TTGTCAATAA CCAGCTGGGT 
TACACCACTC CTGCAGAAAG AGGAAGGTCT TCCTTATACT GTAGTGACAT CGGGAAGCTG 
GTGGGCTGCG CCATCATCCA CGTCAATGGA GACAGCCCAG AGGAAGTGGT GCGGGCCGCA 
CAACTGGCGT TCGAATACCA GCGCCAGTTC CGGAAAGATG TGATTGTTGA CTTGTTGTGC 
TACAGGCAGT GGGGCCACAA TGAGCTGGAC GAGCCCTTCT TCACCAACCC GGGCATGTAC 
CAGATCATCA GAGCCCGGAA GAGCATCCCT GATATGTATG CAGAGTACCT TATGGCCAAC 
GGACTCACAA CCCAGGAGGA GGTGTCGGAA ATCAAAGCCT CCTATTATGT GAAGTTAAAT 
GGCCATTTGA CTCATGCGGC CCACTACAGG CCCCCTGCCA CGAGCCTGCA GGCCCATTGG 
CAAGGCTTGG TTCAGCCGGA AGCTTGTATT ACTTCCTGGA ACACAGGTGT GCCCCTTGAC 
CTCCTGCAGT TTATTGGTAA GAAGTCTGTG GAGGTGCCTG AGGAGTTCCA GATACACAGT 
CACCTTCTGA AGATGCATGT GCAGTCTCGA GTGGAGAAAG TGATGGATGG AACAAAGTTA 
GACTGGGCCA CGGCAGAAGC TCTTGCATTG GGCTCATTGC TTGCTGAAGG TTTTAATGTC 
CGTCTGAGTG GCCAAGATGT GGGCCGTGGA ACTTTTAGCC AGAGGCACGC AATGGTTGTT 
TGCCAGAAGA CAGATGACAC TTATATCCCC CTGAACCATA TGGACCCTAA TCAAAAGGGG 
TTTCTAGAGG TCAGCAACAG CCCACTGTCG GAAGAGGCCG TCCTGGGATT TGAGTATGGG 
ATGAGCATTG AGAGCCCTAA CCTGCTGCCC CTCTGGGAGG CTCAGTTTGG CGATTTCTTC 
AATGGTGCCC AGATCATCTT TGACACGTTC ATCTCTGGAG GCGAGGCCAA GTGGCTCCTC 
CAGAGCGGCA TTGTGGTCCT CCTTCCCCAC GGGTACGACG GGGCTGGACC GGAGCACTCA 
TCTTGTCGAA TAGAGCGTTT CTTGCAGATG TGTGACAGCA CAGAGGAGGG TGTGGATGGG 
GACACTGTGA ACATGTGCGT GGCTCACCCC ACCACGCCCG CCCAGTACTT CCACCTGCTC 
CGGAGACAGA TGATTCGGAA CTTCAGGAAG CCCCTCATCC TTGTTTCTCC GAAGACACTG 
CTCAGACTCC CCGCAGCTGT ATCAACTCTT CAGGAAATGG CACCAGGAAC AACATTCAAA 
CCGGTCATTG GAGATTCATC TGTGGATCCA AAAAAGGTTA AGAGTCTGGT GTTCTGCTGT 
GGCAAACATT TCTATGCCCT GATGAAGCAA AGAGAATCTT TGGAGGCCAG GAAGCATGAC 
TTTGCCATCA TCCGAATAGA AGAACTCTGT CCTTTCCCAC TGGATTCGCT GCAGCAAGAG 
ATGAGCAAAT ACAAACACGT TAGAGATGTT ATCTGGAGTC AAGAGGAGCC TCAGAACATG 
GGTCCTTGGT TTTTTGTTTC TCCAAGGTTT GAGAAGCAGC TGGCCTGCAA GCTCCGTCTT 
GTGAGCCGAC CCGCGTTGCC TGTACCGGCT GTGGGAATCG GCACCATTCA CCTACAGCAG 
CATGAAGAAA TCCTCACTAA GACCTTCAAC CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHTKD1 ( XM_015473957.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329443.1 Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), transcript variant X1, mRNA.

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CloneID	OBa92218
Clone ID Related Accession (Same CDS sequence)	XM_015473957.1 , XM_015473957.2
Accession Version	XM_015473957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGGG TCGCTGTGGC AGCAGCAAGA CTGGGCCTCG GCCGGGCCTC TCCGCTCTTC 
CTCCGGCGCG GCTACCAGAC AGAGAGGGGT GTCTATGGCT ACCGGCCGAG GAAGCCCGAG 
AGCCGGGAGC CCGAGGGCGG CCTGGCGCGC CCCCCAGTGG ACCATGGCCT TGCCAGGTTG 
GTGACGGTGT ATTGTGAGCA TGGGCATAAA GCTGCCAAAA TCAACCCACT CTTCACTGGT 
CAAGCCCTGC AGGAAAATGT GCCTGAAATC CAAGCCCTGG TGCAGACTCT GCAGGGACCC 
TTTAATACCA CAGGATTATT GAACATGGGG AAGGAGGAGG CCTCTCTTGA GGAAGTCGTT 
GCCTATCTCA ACCAGATCTA CTGTGGGCAG ATTTCTATCG AAACCTCTCA GCTTCAGAGC 
CAGGAGGAGA AAGACTGGTT TGCCAAGCGG TTTGAGGAAC TGAAGAAGGA GGCATTTACC 
ACAGAAGAGC GAAAACACCT GTCAAAATTA ATGCTAGAAT CTCAGGAGTT TGACCACTTT 
TTGGCCACCA AGTTTGCCAC AGTGAAGCGA TACGGAGGCG AAGGAGCAGA AAGCATGATG 
GGCTTTTTCT ACGAGCTGCT GAAAATGGCG GCTTACAGCG GAGTAACGGA TGTTATTATT 
GGGATGCCCC ATAGAGGGAG GCTGAACTTG CTGACGGGCC TTCTGCAGTT CCCTCCAGAG 
GTTCATGGTG ATGCTTCTTT CTGTGGTCAA GGGATTGTTC CTGAAACATT TACGCTCTCT 
AATCTCCCGC ATTTCAGAAT TGGTGGGAGT GTCCATTTGA TTGTCAATAA CCAGCTGGGT 
TACACCACTC CTGCAGAAAG AGGAAGGTCT TCCTTATACT GTAGTGACAT CGGGAAGCTG 
GTGGGCTGCG CCATCATCCA CGTCAATGGA GACAGCCCAG AGGAAGTGGT GCGGGCCGCA 
CAACTGGCGT TCGAATACCA GCGCCAGTTC CGGAAAGATG TGATTGTTGA CTTGTTGTGC 
TACAGGCAGT GGGGCCACAA TGAGCTGGAC GAGCCCTTCT TCACCAACCC GGGCATGTAC 
CAGATCATCA GAGCCCGGAA GAGCATCCCT GATATGTATG CAGAGTACCT TATGGCCAAC 
GGACTCACAA CCCAGGAGGA GGTGTCGGAA ATCAAAGCCT CCTATTATGT GAAGTTAAAT 
GGCCATTTGA CTCATGCGGC CCACTACAGG CCCCCTGCCA CGAGCCTGCA GGCCCATTGG 
CAAGGCTTGG TTCAGCCGGA AGCTTGTATT ACTTCCTGGA ACACAGGTGT GCCCCTTGAC 
CTCCTGCAGT TTATTGGTAA GAAGTCTGTG GAGGTGCCTG AGGAGTTCCA GATACACAGT 
CACCTTCTGA AGATGCATGT GCAGTCTCGA GTGGAGAAAG TGATGGATGG AACAAAGTTA 
GACTGGGCCA CGGCAGAAGC TCTTGCATTG GGCTCATTGC TTGCTGAAGG TTTTAATGTC 
CGTCTGAGTG GCCAAGATGT GGGCCGTGGA ACTTTTAGCC AGAGGCACGC AATGGTTGTT 
TGCCAGAAGA CAGATGACAC TTATATCCCC CTGAACCATA TGGACCCTAA TCAAAAGGGG 
TTTCTAGAGG TCAGCAACAG CCCACTGTCG GAAGAGGCCG TCCTGGGATT TGAGTATGGG 
ATGAGCATTG AGAGCCCTAA CCTGCTGCCC CTCTGGGAGG CTCAGTTTGG CGATTTCTTC 
AATGGTGCCC AGATCATCTT TGACACGTTC ATCTCTGGAG GCGAGGCCAA GTGGCTCCTC 
CAGAGCGGCA TTGTGGTCCT CCTTCCCCAC GGGTACGACG GGGCTGGACC GGAGCACTCA 
TCTTGTCGAA TAGAGCGTTT CTTGCAGATG TGTGACAGCA CAGAGGAGGG TGTGGATGGG 
GACACTGTGA ACATGTGCGT GGCTCACCCC ACCACGCCCG CCCAGTACTT CCACCTGCTC 
CGGAGACAGA TGATTCGGAA CTTCAGGAAG CCCCTCATCC TTGTTTCTCC GAAGACACTG 
CTCAGACTCC CCGCAGCTGT ATCAACTCTT CAGGAAATGG CACCAGGAAC AACATTCAAA 
CCGGTCATTG GAGATTCATC TGTGGATCCA AAAAAGGTTA AGAGTCTGGT GTTCTGCTGT 
GGCAAACATT TCTATGCCCT GATGAAGCAA AGAGAATCTT TGGAGGCCAG GAAGCATGAC 
TTTGCCATCA TCCGAATAGA AGAACTCTGT CCTTTCCCAC TGGATTCGCT GCAGCAAGAG 
ATGAGCAAAT ACAAACACGT TAGAGATGTT ATCTGGAGTC AAGAGGAGCC TCAGAACATG 
GGTCCTTGGT TTTTTGTTTC TCCAAGGTTT GAGAAGCAGC TGGCCTGCAA GCTCCGTCTT 
GTGAGCCGAC CCGCGTTGCC TGTACCGGCT GTGGGAATCG GCACCATTCA CCTACAGCAG 
CATGAAGAAA TCCTCACTAA GACCTTCAAC CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329443.1
CDS	128..2623
Translation	MAWVAVAAARLGLGRASPLFLRRGYQTERGVYGYRPRKPESREPEGGLARPPVDHGLARLVTVYCEHGHKAAKINPLFTGQALQENVPEIQALVQTLQGPFNTTGLLNMGKEEASLEEVVAYLNQIYCGQISIETSQLQSQEEKDWFAKRFEELKKEAFTTEERKHLSKLMLESQEFDHFLATKFATVKRYGGEGAESMMGFFYELLKMAAYSGVTDVIIGMPHRGRLNLLTGLLQFPPEVHGDASFCGQGIVPETFTLSNLPHFRIGGSVHLIVNNQLGYTTPAERGRSSLYCSDIGKLVGCAIIHVNGDSPEEVVRAAQLAFEYQRQFRKDVIVDLLCYRQWGHNELDEPFFTNPGMYQIIRARKSIPDMYAEYLMANGLTTQEEVSEIKASYYVKLNGHLTHAAHYRPPATSLQAHWQGLVQPEACITSWNTGVPLDLLQFIGKKSVEVPEEFQIHSHLLKMHVQSRVEKVMDGTKLDWATAEALALGSLLAEGFNVRLSGQDVGRGTFSQRHAMVVCQKTDDTYIPLNHMDPNQKGFLEVSNSPLSEEAVLGFEYGMSIESPNLLPLWEAQFGDFFNGAQIIFDTFISGGEAKWLLQSGIVVLLPHGYDGAGPEHSSCRIERFLQMCDSTEEGVDGDTVNMCVAHPTTPAQYFHLLRRQMIRNFRKPLILVSPKTLLRLPAAVSTLQEMAPGTTFKPVIGDSSVDPKKVKSLVFCCGKHFYALMKQRESLEARKHDFAIIRIEELCPFPLDSLQQEMSKYKHVRDVIWSQEEPQNMGPWFFVSPRFEKQLACKLRLVSRPALPVPAVGIGTIHLQQHEEILTKTFNP

Target ORF information:

RefSeq Version	XM_015473957.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473957.2

ORF Insert Sequence:

ATGGCCTGGG TCGCTGTGGC AGCAGCAAGA CTGGGCCTCG GCCGGGCCTC TCCGCTCTTC 
CTCCGGCGCG GCTACCAGAC AGAGAGGGGT GTCTATGGCT ACCGGCCGAG GAAGCCCGAG 
AGCCGGGAGC CCGAGGGCGG CCTGGCGCGC CCCCCAGTGG ACCATGGCCT TGCCAGGTTG 
GTGACGGTGT ATTGTGAGCA TGGGCATAAA GCTGCCAAAA TCAACCCACT CTTCACTGGT 
CAAGCCCTGC AGGAAAATGT GCCTGAAATC CAAGCCCTGG TGCAGACTCT GCAGGGACCC 
TTTAATACCA CAGGATTATT GAACATGGGG AAGGAGGAGG CCTCTCTTGA GGAAGTCGTT 
GCCTATCTCA ACCAGATCTA CTGTGGGCAG ATTTCTATCG AAACCTCTCA GCTTCAGAGC 
CAGGAGGAGA AAGACTGGTT TGCCAAGCGG TTTGAGGAAC TGAAGAAGGA GGCATTTACC 
ACAGAAGAGC GAAAACACCT GTCAAAATTA ATGCTAGAAT CTCAGGAGTT TGACCACTTT 
TTGGCCACCA AGTTTGCCAC AGTGAAGCGA TACGGAGGCG AAGGAGCAGA AAGCATGATG 
GGCTTTTTCT ACGAGCTGCT GAAAATGGCG GCTTACAGCG GAGTAACGGA TGTTATTATT 
GGGATGCCCC ATAGAGGGAG GCTGAACTTG CTGACGGGCC TTCTGCAGTT CCCTCCAGAG 
GTTCATGGTG ATGCTTCTTT CTGTGGTCAA GGGATTGTTC CTGAAACATT TACGCTCTCT 
AATCTCCCGC ATTTCAGAAT TGGTGGGAGT GTCCATTTGA TTGTCAATAA CCAGCTGGGT 
TACACCACTC CTGCAGAAAG AGGAAGGTCT TCCTTATACT GTAGTGACAT CGGGAAGCTG 
GTGGGCTGCG CCATCATCCA CGTCAATGGA GACAGCCCAG AGGAAGTGGT GCGGGCCGCA 
CAACTGGCGT TCGAATACCA GCGCCAGTTC CGGAAAGATG TGATTGTTGA CTTGTTGTGC 
TACAGGCAGT GGGGCCACAA TGAGCTGGAC GAGCCCTTCT TCACCAACCC GGGCATGTAC 
CAGATCATCA GAGCCCGGAA GAGCATCCCT GATATGTATG CAGAGTACCT TATGGCCAAC 
GGACTCACAA CCCAGGAGGA GGTGTCGGAA ATCAAAGCCT CCTATTATGT GAAGTTAAAT 
GGCCATTTGA CTCATGCGGC CCACTACAGG CCCCCTGCCA CGAGCCTGCA GGCCCATTGG 
CAAGGCTTGG TTCAGCCGGA AGCTTGTATT ACTTCCTGGA ACACAGGTGT GCCCCTTGAC 
CTCCTGCAGT TTATTGGTAA GAAGTCTGTG GAGGTGCCTG AGGAGTTCCA GATACACAGT 
CACCTTCTGA AGATGCATGT GCAGTCTCGA GTGGAGAAAG TGATGGATGG AACAAAGTTA 
GACTGGGCCA CGGCAGAAGC TCTTGCATTG GGCTCATTGC TTGCTGAAGG TTTTAATGTC 
CGTCTGAGTG GCCAAGATGT GGGCCGTGGA ACTTTTAGCC AGAGGCACGC AATGGTTGTT 
TGCCAGAAGA CAGATGACAC TTATATCCCC CTGAACCATA TGGACCCTAA TCAAAAGGGG 
TTTCTAGAGG TCAGCAACAG CCCACTGTCG GAAGAGGCCG TCCTGGGATT TGAGTATGGG 
ATGAGCATTG AGAGCCCTAA CCTGCTGCCC CTCTGGGAGG CTCAGTTTGG CGATTTCTTC 
AATGGTGCCC AGATCATCTT TGACACGTTC ATCTCTGGAG GCGAGGCCAA GTGGCTCCTC 
CAGAGCGGCA TTGTGGTCCT CCTTCCCCAC GGGTACGACG GGGCTGGACC GGAGCACTCA 
TCTTGTCGAA TAGAGCGTTT CTTGCAGATG TGTGACAGCA CAGAGGAGGG TGTGGATGGG 
GACACTGTGA ACATGTGCGT GGCTCACCCC ACCACGCCCG CCCAGTACTT CCACCTGCTC 
CGGAGACAGA TGATTCGGAA CTTCAGGAAG CCCCTCATCC TTGTTTCTCC GAAGACACTG 
CTCAGACTCC CCGCAGCTGT ATCAACTCTT CAGGAAATGG CACCAGGAAC AACATTCAAA 
CCGGTCATTG GAGATTCATC TGTGGATCCA AAAAAGGTTA AGAGTCTGGT GTTCTGCTGT 
GGCAAACATT TCTATGCCCT GATGAAGCAA AGAGAATCTT TGGAGGCCAG GAAGCATGAC 
TTTGCCATCA TCCGAATAGA AGAACTCTGT CCTTTCCCAC TGGATTCGCT GCAGCAAGAG 
ATGAGCAAAT ACAAACACGT TAGAGATGTT ATCTGGAGTC AAGAGGAGCC TCAGAACATG 
GGTCCTTGGT TTTTTGTTTC TCCAAGGTTT GAGAAGCAGC TGGCCTGCAA GCTCCGTCTT 
GTGAGCCGAC CCGCGTTGCC TGTACCGGCT GTGGGAATCG GCACCATTCA CCTACAGCAG 
CATGAAGAAA TCCTCACTAA GACCTTCAAC CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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DHTKD1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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