uba1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	uba1
Entrez Gene ID	406335
Full Name	ubiquitin-like modifier activating enzyme 1
Synonyms	ube1,wu:fa01e08,wu:fb30f01,wu:fi21c11,wu:fj14g11,zgc:66143
General protein information	Preferred Names ubiquitin-like modifier activating enzyme 1 Names ubiquitin-like modifier-activating enzyme 1 fb30f01 ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005162051.4	XP_005162108.1	ubiquitin-like modifier-activating enzyme 1 isoform X1
NM_213062.1	NP_998227.1	ubiquitin-like modifier-activating enzyme 1
XM_005162051.3	XP_005162108.1	ubiquitin-like modifier-activating enzyme 1 isoform X1
XM_005162052.3	XP_005162109.1	ubiquitin-like modifier-activating enzyme 1 isoform X1
XM_005162052.4	XP_005162109.1	ubiquitin-like modifier-activating enzyme 1 isoform X1

Pathways from BioSystems

Homologies

	UBA1	XP_001092372.2
Mus musculus (house mouse)	Uba1	NP_033483.2
Drosophila melanogaster (fruit fly)	Uba1	NP_477310.2
Saccharomyces cerevisiae (baker's yeast)	UBA1	NP_012712.1
Homo sapiens (human)	UBA1	NP_003325.2
Canis lupus familiaris (dog)	UBA1	XP_005641334.1
Bos taurus (cattle)	UBA1	NP_001095947.1
Danio rerio (zebrafish)	uba1	NP_998227.1
Caenorhabditis elegans (roundworm)	uba-1	NP_001033404.1
Oryza sativa (rice)	Os03g0294900	NP_001049821.1
Schizosaccharomyces pombe (fission yeast)	ptr3	XP_001713148.1
Magnaporthe oryzae (rice blast fungus)	MGG_01409	XP_003714354.1
Oryza sativa (rice)	Os07g0692900	NP_001060725.1
Rattus norvegicus (Norway rat)	Uba1	NP_001014102.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011872	XP_552371.3
Kluyveromyces lactis	KLLA0B14278g	XP_452166.1
Eremothecium gossypii	AGOS_AFR433C	NP_985980.1
Neurospora crassa	NCU04370	XP_956143.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Dysregulation of ubiquitin homeostasis and ?-catenin signaling promote spinal muscular atrophy.
Wishart TM, Mutsaers CA, Riessland M, Reimer MM, Hunter G, Hannam ML, Eaton SL, Fuller HR, Roche SL, Somers E, Morse R, Young PJ, Lamont DJ, Hammerschmidt M, Joshi A, Hohenstein P, Morris GE, Parson SH, Skehel PA, Becker T, Robinson IM, Becker CG, Wirth B, Gillingwater TH
The Journal of clinical investigation124(4)1821-34(2014 Apr)

Global identification of SMAD2 target genes reveals a role for multiple co-regulatory factors in zebrafish early gastrulas.
Liu Z, Lin X, Cai Z, Zhang Z, Han C, Jia S, Meng A, Wang Q
The Journal of biological chemistry286(32)28520-32(2011 Aug)

Transcriptome kinetics of arsenic-induced adaptive response in zebrafish liver.
Lam SH, Winata CL, Tong Y, Korzh S, Lim WS, Korzh V, Spitsbergen J, Mathavan S, Miller LD, Liu ET, Gong Z
Physiological genomics27(3)351-61(2006 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following uba1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the uba1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34988	NM_213062.1 Latest version!	Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa27570	XM_005162051.4 Latest version!	Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa27570	XM_005162052.4 Latest version!	Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa27570	XM_005162051.3	Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa27570	XM_005162052.3	Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

uba1 ( NM_213062.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998227.1 Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), mRNA.

Price & Availability↑

CloneID	ODa34988
Clone ID Related Accession (Same CDS sequence)	NM_213062.1
Accession Version	NM_213062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	ubiquitin-like modifier-activating enzyme 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC060674.1. ##Evidence-Data-START## Transcript exon combination :: BC060674.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505391, SAMEA4476754 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAGCT CGCCGCTGTC CAAGAAGCGT CGCGTGTCAG GATCAGAGAC GAAGACGGGA 
TCCCATTGCT CCTCCTCTAA CTCTGTCAGA ACTGAACTGT CACACACACC AGCTAACGGC 
ATGGCTAAGA ATGGAAATGA TGCTGAGATC GACGAGGGCC TGTACTCCAG ACAGCTGTAC 
GTGCTGGGTC ATGATGCAAT GAAGCGTATG CAGAGCTCTA ATGTCCTTAT CTCGGGACTG 
CGAGGTTTAG GGGTAGAGAT CGCTAAGAAT GTCATTCTGG GTGGAGTCAA AAGCGTCACC 
CTGCACGATC AGGGAGTGGC CGAGTGGAAG GACCTTTCAT CACAGTTTTA CCTGCGTGAG 
GAGGATCTTG GGAAGAACCG TGCTGATGTC AGTCAGCCTC GACTGGCAGA ACTGAACAGT 
TATGTGCCTG TAACCTCCTA CACTGGCACC CTCACCAATG AATACCTGAC CAAATTTCAG 
GTTGTTGTGC TCACCAACTC TAGTCTTGAT GAACAGACAC GTATCGGGGA ATTTTGCCAC 
AGTAATGGCA TCAAGTTGAT TGTGGCAGAC ACACGTGGAC TGTTTGGGCA ACTGTTCTGT 
GATTTTGGGG AGGTAATGAT AGTATTTGAC ACAAACGGAG AACAGCCATT GAGCGCCATG 
ATCTCAATGA TCACAAAAGA CAGCGCTGGT GTGGTTACGT GTCTGGATGA AGCACGGCAC 
GGATTTGAGA GCGGAGACTA TGTCACTTTC ACAGAAGTGC AGGGCATGAC GGAACTTAAT 
GGCTGTGATC CAGTAGAGAT CAAGACACTG GGTCCTTACA CTTTCAGCAT CTGTGACACC 
AGCTCATTCT CTGACTATGT GAGAGGTGGT ATTGTCACAC AAGTGAAGAT GCCCAAGAAG 
ATTGCCTTTA AATCCCTGTC CTCCTCCATG GCCGAACCAG AGTTCTTGCT GACTGATTTT 
GCCAAATTTG ATCGTCCAGG CCAACTCCAC GTGGGCTTTC AGGCACTGCA TGCATTTGAG 
AAGAAGCACA GTCGTCTGCC AAAGCCCTGG AATCAGGCAG ATGCGGATGA GCTTATGACT 
CTGGCTGAAG AGGTGAATGC TGCACAGACA GGATCTGCCA AGCAAGAGGA GCTGGACCAG 
GCAGTCATAA AAAAGCTGGC CTGCGTGGCT GCTGGAGACC TGGCACCCGT CAATGCCTTT 
ATTGGTGGGC TTGCTGCCCA GGAGGTTATG AAGGCCTGCA CCGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTATTTTGA TGCTTTGGAG TGCTTACCTG AGCCTGAGGA AGTCATTCTT 
ACAGAGGAAG AGTGTGCTCC TAGGAACTGC CGGTATGACG GCCAGATTGC CGTCTTTGGG 
TCCAAGCTGC AGGAGCTGTT AGCCAAGCAG AGATATTTTC TGGTTGGTGC TGGAGCCATT 
GGCTGTGAGC TGCTGAAGAA CTTTGCTATG ATGGGCTTGG CCAGTGGTGA AGGAGAGGTC 
ATAGTCACTG ACATGGACAC CATTGAGAAG TCCAATCTCA ATCGACAGTT CCTCTTCAGA 
CCCTGGGATG TCACGAAGAT GAAGAGTGAG ACTGCTGCTG CGGCAGTGAA GCAGATGAAC 
CCGTCAGTTC GTATCACTGG TCACCAGAAC CGTGTGGGAC CGGACACGGA GAAGGTCTAT 
GATGATGACT TTTTTGAATG CCTTGATGGA GTGGCAAATG CTCTTGACAA TGTAGATGCC 
CGTATGTATA TGGATCGGAG GTGTGTGTAC TACCGTAAGC CATTGCTGGA GTCTGGTACT 
CTTGGCACCA AAGGAAACGT GCAGGTGGTC ATTCCCTTCA TCACAGAGTC CTACAGCTCC 
AGCCAGGATC CTCCAGAGAA GTCCATTCCT ATCTGCACAC TCAAGAACTT CCCCAATGCA 
ATCGAACACA CCCTACAGTG GGCTCGTGAT GAGTTTGAGG GACTGTTCAA GCAACCTGCT 
GAAAACGCCT TGCAGTATCT GACAGACTCA AAGTTTATGG AGCGCACACT GAAGCTCCCT 
GGAGCTCAGC CGCTGGAGGT GGTGGAATCG GTTTACAAGA GTCTGGTGAC AGACAGGCCA 
CGCAACTGGG ATGACTGTGT AACCTGGGCC CGCAACCACT GGCAGTGTCA ATATAACAAT 
AACATCCGTC AGCTGCTGCA CAATTTCCCC CCAGACCAGC TTACAAGCTC TGGGGCGCCA 
TTCTGGTCAG GTCCAAAGAG ATGTCCTCAT CCATTAGAAT TCAGCACTAA CAATGATCTT 
CACATGGACT ACATTTTGGC AGCAGCTAAC TTGTATGCCC TGTCATACGG CCTGCCATCC 
TGCAATGACC GCTCAGCTCT GACCAAGCTT TTGCAGGATA TTAAAGTGCC TGAGTTCACA 
CCCAAGTCAG GAGTCAAGAT TCACGTGTCT GATCAGGAGC TGCAGAGTGC CAACGCATCA 
GTCGATGACT CAAGGCTTGA AGAGCTGAAG ACACTGTTGC CTTCTTTAGA AGCATCTTCT 
CAATTCAAGC TCTGTCCTAT TGAATTTGAA AAGGACGATG ACACAAACTT CCACATGGAC 
TTCATTGTAG CGGCATCTAA TCTGAGAGCA GAGAACTATG ACATTCCTCC TGCAGACAGA 
CACAAGAGCA AACTCATTGC TGGAAAAATA ATTCCAGCCA TCGCCACAAC AACAGCCGCT 
GTTGTAGGTC TGGTGTGTCT GGAGTTGCTG AAGATCGTTC AGGGGCACAA GAAGCTCGAG 
TCCTACAAAA ATGGCTTCAT GAACCTTGCT CTGCCTTTCT TTGCCTTCTC CGAGCCTATA 
GCTGCCCCCA AGCACAAGTA CTACGAGATT GATTGGACAC TTTGGGACCG TTTTAAGGTG 
AAGGGCATTC AGCCTAATGG AGAGGAAATG ACACTCCGGC AGTTCCTGGA TTATTTCAAG 
AATGAACACA AACTGGAGAT CACGATGCTC TCTCAGGGAG TTTCTATGCT CTACTCTTTC 
TTCATGCCTG CAGCCAAACT CAAAGAAAGA CTGGAACTAC CGATGACTGA GATCGTGACC 
AAAGTGTCCA AGAAGAAACT GGGCAAGCAT GTCAAAGCAC TAGTGTTTGA GCTCTGCTGC 
AATGACGACA CAGAAGAAGA CGTAGAGGTG CCCTACGTCA GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998227.1
CDS	127..3303
Translation	MSSSPLSKKRRVSGSETKTGSHCSSSNSVRTELSHTPANGMAKNGNDAEIDEGLYSRQLYVLGHDAMKRMQSSNVLISGLRGLGVEIAKNVILGGVKSVTLHDQGVAEWKDLSSQFYLREEDLGKNRADVSQPRLAELNSYVPVTSYTGTLTNEYLTKFQVVVLTNSSLDEQTRIGEFCHSNGIKLIVADTRGLFGQLFCDFGEVMIVFDTNGEQPLSAMISMITKDSAGVVTCLDEARHGFESGDYVTFTEVQGMTELNGCDPVEIKTLGPYTFSICDTSSFSDYVRGGIVTQVKMPKKIAFKSLSSSMAEPEFLLTDFAKFDRPGQLHVGFQALHAFEKKHSRLPKPWNQADADELMTLAEEVNAAQTGSAKQEELDQAVIKKLACVAAGDLAPVNAFIGGLAAQEVMKACTGKFMPIMQWLYFDALECLPEPEEVILTEEECAPRNCRYDGQIAVFGSKLQELLAKQRYFLVGAGAIGCELLKNFAMMGLASGEGEVIVTDMDTIEKSNLNRQFLFRPWDVTKMKSETAAAAVKQMNPSVRITGHQNRVGPDTEKVYDDDFFECLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFITESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENALQYLTDSKFMERTLKLPGAQPLEVVESVYKSLVTDRPRNWDDCVTWARNHWQCQYNNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLEFSTNNDLHMDYILAAANLYALSYGLPSCNDRSALTKLLQDIKVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKTLLPSLEASSQFKLCPIEFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELLKIVQGHKKLESYKNGFMNLALPFFAFSEPIAAPKHKYYEIDWTLWDRFKVKGIQPNGEEMTLRQFLDYFKNEHKLEITMLSQGVSMLYSFFMPAAKLKERLELPMTEIVTKVSKKKLGKHVKALVFELCCNDDTEEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	NM_213062.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213062.1

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAGCGT CGCGTGTCAG GATCAGAGAC GAAGACGGGA 
TCCCATTGCT CCTCCTCTAA CTCTGTCAGA ACTGAACTGT CACACACACC AGCTAACGGC 
ATGGCTAAGA ATGGAAATGA TGCTGAGATC GACGAGGGCC TGTACTCCAG ACAGCTGTAC 
GTGCTGGGTC ATGATGCAAT GAAGCGTATG CAGAGCTCTA ATGTCCTTAT CTCGGGACTG 
CGAGGTTTAG GGGTAGAGAT CGCTAAGAAT GTCATTCTGG GTGGAGTCAA AAGCGTCACC 
CTGCACGATC AGGGAGTGGC CGAGTGGAAG GACCTTTCAT CACAGTTTTA CCTGCGTGAG 
GAGGATCTTG GGAAGAACCG TGCTGATGTC AGTCAGCCTC GACTGGCAGA ACTGAACAGT 
TATGTGCCTG TAACCTCCTA CACTGGCACC CTCACCAATG AATACCTGAC CAAATTTCAG 
GTTGTTGTGC TCACCAACTC TAGTCTTGAT GAACAGACAC GTATCGGGGA ATTTTGCCAC 
AGTAATGGCA TCAAGTTGAT TGTGGCAGAC ACACGTGGAC TGTTTGGGCA ACTGTTCTGT 
GATTTTGGGG AGGTAATGAT AGTATTTGAC ACAAACGGAG AACAGCCATT GAGCGCCATG 
ATCTCAATGA TCACAAAAGA CAGCGCTGGT GTGGTTACGT GTCTGGATGA AGCACGGCAC 
GGATTTGAGA GCGGAGACTA TGTCACTTTC ACAGAAGTGC AGGGCATGAC GGAACTTAAT 
GGCTGTGATC CAGTAGAGAT CAAGACACTG GGTCCTTACA CTTTCAGCAT CTGTGACACC 
AGCTCATTCT CTGACTATGT GAGAGGTGGT ATTGTCACAC AAGTGAAGAT GCCCAAGAAG 
ATTGCCTTTA AATCCCTGTC CTCCTCCATG GCCGAACCAG AGTTCTTGCT GACTGATTTT 
GCCAAATTTG ATCGTCCAGG CCAACTCCAC GTGGGCTTTC AGGCACTGCA TGCATTTGAG 
AAGAAGCACA GTCGTCTGCC AAAGCCCTGG AATCAGGCAG ATGCGGATGA GCTTATGACT 
CTGGCTGAAG AGGTGAATGC TGCACAGACA GGATCTGCCA AGCAAGAGGA GCTGGACCAG 
GCAGTCATAA AAAAGCTGGC CTGCGTGGCT GCTGGAGACC TGGCACCCGT CAATGCCTTT 
ATTGGTGGGC TTGCTGCCCA GGAGGTTATG AAGGCCTGCA CCGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTATTTTGA TGCTTTGGAG TGCTTACCTG AGCCTGAGGA AGTCATTCTT 
ACAGAGGAAG AGTGTGCTCC TAGGAACTGC CGGTATGACG GCCAGATTGC CGTCTTTGGG 
TCCAAGCTGC AGGAGCTGTT AGCCAAGCAG AGATATTTTC TGGTTGGTGC TGGAGCCATT 
GGCTGTGAGC TGCTGAAGAA CTTTGCTATG ATGGGCTTGG CCAGTGGTGA AGGAGAGGTC 
ATAGTCACTG ACATGGACAC CATTGAGAAG TCCAATCTCA ATCGACAGTT CCTCTTCAGA 
CCCTGGGATG TCACGAAGAT GAAGAGTGAG ACTGCTGCTG CGGCAGTGAA GCAGATGAAC 
CCGTCAGTTC GTATCACTGG TCACCAGAAC CGTGTGGGAC CGGACACGGA GAAGGTCTAT 
GATGATGACT TTTTTGAATG CCTTGATGGA GTGGCAAATG CTCTTGACAA TGTAGATGCC 
CGTATGTATA TGGATCGGAG GTGTGTGTAC TACCGTAAGC CATTGCTGGA GTCTGGTACT 
CTTGGCACCA AAGGAAACGT GCAGGTGGTC ATTCCCTTCA TCACAGAGTC CTACAGCTCC 
AGCCAGGATC CTCCAGAGAA GTCCATTCCT ATCTGCACAC TCAAGAACTT CCCCAATGCA 
ATCGAACACA CCCTACAGTG GGCTCGTGAT GAGTTTGAGG GACTGTTCAA GCAACCTGCT 
GAAAACGCCT TGCAGTATCT GACAGACTCA AAGTTTATGG AGCGCACACT GAAGCTCCCT 
GGAGCTCAGC CGCTGGAGGT GGTGGAATCG GTTTACAAGA GTCTGGTGAC AGACAGGCCA 
CGCAACTGGG ATGACTGTGT AACCTGGGCC CGCAACCACT GGCAGTGTCA ATATAACAAT 
AACATCCGTC AGCTGCTGCA CAATTTCCCC CCAGACCAGC TTACAAGCTC TGGGGCGCCA 
TTCTGGTCAG GTCCAAAGAG ATGTCCTCAT CCATTAGAAT TCAGCACTAA CAATGATCTT 
CACATGGACT ACATTTTGGC AGCAGCTAAC TTGTATGCCC TGTCATACGG CCTGCCATCC 
TGCAATGACC GCTCAGCTCT GACCAAGCTT TTGCAGGATA TTAAAGTGCC TGAGTTCACA 
CCCAAGTCAG GAGTCAAGAT TCACGTGTCT GATCAGGAGC TGCAGAGTGC CAACGCATCA 
GTCGATGACT CAAGGCTTGA AGAGCTGAAG ACACTGTTGC CTTCTTTAGA AGCATCTTCT 
CAATTCAAGC TCTGTCCTAT TGAATTTGAA AAGGACGATG ACACAAACTT CCACATGGAC 
TTCATTGTAG CGGCATCTAA TCTGAGAGCA GAGAACTATG ACATTCCTCC TGCAGACAGA 
CACAAGAGCA AACTCATTGC TGGAAAAATA ATTCCAGCCA TCGCCACAAC AACAGCCGCT 
GTTGTAGGTC TGGTGTGTCT GGAGTTGCTG AAGATCGTTC AGGGGCACAA GAAGCTCGAG 
TCCTACAAAA ATGGCTTCAT GAACCTTGCT CTGCCTTTCT TTGCCTTCTC CGAGCCTATA 
GCTGCCCCCA AGCACAAGTA CTACGAGATT GATTGGACAC TTTGGGACCG TTTTAAGGTG 
AAGGGCATTC AGCCTAATGG AGAGGAAATG ACACTCCGGC AGTTCCTGGA TTATTTCAAG 
AATGAACACA AACTGGAGAT CACGATGCTC TCTCAGGGAG TTTCTATGCT CTACTCTTTC 
TTCATGCCTG CAGCCAAACT CAAAGAAAGA CTGGAACTAC CGATGACTGA GATCGTGACC 
AAAGTGTCCA AGAAGAAACT GGGCAAGCAT GTCAAAGCAC TAGTGTTTGA GCTCTGCTGC 
AATGACGACA CAGAAGAAGA CGTAGAGGTG CCCTACGTCA GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uba1 ( XM_005162051.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162108.1 Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27570
Clone ID Related Accession (Same CDS sequence)	XM_005162051.4 , XM_005162051.3 , XM_005162052.3 , XM_005162052.4
Accession Version	XM_005162051.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin-like modifier-activating enzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162051.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT CGCCGCTGTC CAAGAAGCGT CGCGTGTCAG GATCAGAGAC GAAGACGGGA 
TCCCATTGCT CCTCCTCTAA CTCTGTCAGA ACTGAACTGT CACACACACC AGCTAACGGC 
ATGGCTAAGA ATGGAAATGA TGCTGAGATC GACGAGGGCC TGTACTCCAG ACAGCTGTAC 
GTGCTGGGTC ATGATGCTAT GAAGCGTATG CAGAGCTCTA ATGTTCTTAT CTCGGGACTG 
CGAGGTTTAG GGGTAGAGAT CGCTAAGAAT GTCATTCTGG GTGGAGTCAA AAGCGTCACC 
CTGCACGATC AGGGAGTGGC CGAGTGGAAG GACCTTTCAT CACAGTTTTA CCTGCGTGAG 
GAGGATCTTG GGAAGAACCG TGCTGATGTC AGTCAGCCTC GACTGGCAGA ACTGAACAGT 
TATGTGCCTG TAACCTCCTA CACTGGCACC CTCACCAATG AATACCTGAC CAAATTTCAG 
GTTGTTGTGC TCACCAACTC TAGTCTTGAT GAACAGACAC GTATCGGGGA ATTTTGCCAC 
AGTAATGGCA TCAAGTTGAT TGTGGCAGAC ACACGTGGAC TGTTTGGGCA ACTGTTCTGT 
GATTTTGGGG AGGAAATGAT AGTATTCGAC ACAAACGGAG AACAGCCATT GAGCGCCATG 
ATCTCAATGA TCACAAAAGA CAGCGCTGGT GTGGTTACGT GTCTGGATGA AGCACGGCAC 
GGATTTGAGA GCGGAGACTA TGTCACTTTC ACAGAAGTGC AGGGCATGAC GGAACTTAAT 
GGCTGTGATC CAGTAGAGAT CAAGACACTG GGTCCTTACA CTTTCAGCAT CTGTGACACC 
AGCTCATTCT CTGACTATGT GAGAGGTGGT ATTGTCACAC AAGTGAAGAT GCCCAAGAAG 
ATTGCCTTTA AATCCCTGTC CTCCTCCATG GCCGAACCAG AGTTCTTGCT GACTGATTTT 
GCCAAATTTG ATCGTCCAGG CCAACTCCAC GTGGGCTTTC AGGCACTGCA TGCATTTGAG 
AAGAAGCACA GTCGTCTGCC AAAGCCCTGG AATCAGGCAG ATGCGGATGA GCTTATGACT 
CTGGCTGAAG AGGTGAATGC TGCACAGACA GGATCTGCCA AGCAAGAGGA GCTGGACCAG 
GCAGTCATAA AAAAGCTGGC CTGCGTGGCT GCTGGAGACC TGGCACCCGT CAATGCCTTT 
ATTGGTGGGC TTGCTGCCCA GGAGGTTATG AAGGCCTGCA CAGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTATTTTGA TGCTTTGGAG TGCTTACCTG AGCCTGAGGA AGTCATTCTT 
ACAGAGGAAG AGTGTGCTCC TAGGAACTGC CGGTATGACG GCCAGATTGC CGTCTTTGGG 
TCCAAGCTGC AGGAGCTGTT AGCCAAGCAG AGATATTTTC TGGTTGGTGC TGGAGCCATT 
GGCTGTGAGC TGCTGAAGAA CTTTGCTATG ATGGGCTTGG CCAGTGGTGA AGGAGAGGTC 
ATAGTCACTG ACATGGACAC CATTGAGAAG TCCAATCTCA ATCGACAGTT CCTCTTCAGA 
CCCTGGGATG TCACGAAGAT GAAGAGTGAG ACTGCTGCTG CGGCAGTGAA GCAGATGAAC 
CCGTCAGTCC GTATCACTGG TCACCAGAAC CGTGTGGGAC CGGACACGGA GAAGGTCTAT 
GATGATGACT TTTTTGAATG CCTTGATGGA GTGGCAAATG CTCTTGACAA TGTAGATGCC 
CGTATGTATA TGGATCGGAG GTGTGTGTAC TACCGTAAGC CATTGCTGGA GTCTGGTACT 
CTTGGCACCA AAGGAAACGT GCAGGTGGTC ATTCCCTTCA TCACAGAGTC CTACAGCTCC 
AGCCAGGATC CTCCAGAGAA GTCCATTCCT ATCTGCACAC TCAAGAACTT CCCAAATGCA 
ATCGAACACA CCCTACAGTG GGCTCGTGAT GAGTTTGAGG GACTGTTCAA GCAACCTGCT 
GAAAACGCCT TGCAGTATCT GACAGACTCA AAGTTTATGG AGCGCACACT GAAGCTCCCT 
GGAGCTCAGC CGCTGGAGGT GGTGGAATCG GTTTACAAGA GTCTGGTGAC AGACAGGCCA 
CGCAACTGGG ATGACTGTGT AACCTGGGCC CGCAACCACT GGCAGTGTCA ATATAACAAT 
AACATCCGTC AGCTGCTGCA CAATTTCCCC CCAGACCAGC TTACAAGCTC TGGGGCGCCA 
TTCTGGTCAG GTCCAAAGAG ATGTCCTCAT CCATTAGAAT TCAGCACTAA CAATGATCTT 
CACATGGACT ACATTTTGGC AGCAGCTAAC TTGTATGCCC TGTCATACGG CCTGCCATCC 
TGCAATGACC GCTCAGCTCT GACCAAGCTT TTGCAGGATA TTAAAGTGCC TGAGTTCACA 
CCCAAGTCAG GAGTCAAGAT TCACGTGTCT GATCAGGAGC TGCAGAGTGC CAACGCATCA 
GTCGATGACT CGAGGCTCGA AGAGCTGAAG ACACTGTTGC CTTCTTTAGA AGCATCTTCT 
CAATTCAAGC TCTGTCCTAT TGAATTTGAA AAGGACGATG ACACAAACTT CCACATGGAC 
TTCATTGTAG CGGCATCTAA TCTGAGAGCA GAGAACTATG ACATTCCTCC TGCAGACAGA 
CACAAGAGCA AACTCATTGC TGGAAAAATC ATTCCAGCCA TTGCCACAAC AACAGCCGCT 
GTTGTAGGTC TGGTGTGTCT GGAGTTGCTG AAGATCGTTC AGGGGCACAA GAAGCTCGAG 
TCCTACAAAA ATGGCTTCAT GAACCTTGCT CTGCCTTTCT TTGCCTTCTC CGAGCCTATA 
GCTGCCCCCA AGCACAAGTA CTACGAGATT GATTGGACAC TTTGGGACCG TTTTGAGGTG 
AAGGGCATTC AGCCTAATGG AGAGGAAATG ACACTCCGGC AGTTCCTGGA TTATTTCAAG 
AATGAACACA AACTGGAGAT CACGATGCTC TCTCAGGGAG TTTCTATGCT CTACTCTTTC 
TTCATGCCTG CAGCCAAACT CAAAGAAAGA CTGGAACTAC CGATGACTGA GATCGTGACC 
AAAGTGTCCA AGAAGAAACT GGGCAAGCAT GTCAAAGCAC TAGTGTTTGA GCTCTGCTGC 
AATGACGACA CAGAAGAAGA CGTAGAGGTG CCCTATGTCA GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162108.1
CDS	275..3451
Translation	MSSSPLSKKRRVSGSETKTGSHCSSSNSVRTELSHTPANGMAKNGNDAEIDEGLYSRQLYVLGHDAMKRMQSSNVLISGLRGLGVEIAKNVILGGVKSVTLHDQGVAEWKDLSSQFYLREEDLGKNRADVSQPRLAELNSYVPVTSYTGTLTNEYLTKFQVVVLTNSSLDEQTRIGEFCHSNGIKLIVADTRGLFGQLFCDFGEEMIVFDTNGEQPLSAMISMITKDSAGVVTCLDEARHGFESGDYVTFTEVQGMTELNGCDPVEIKTLGPYTFSICDTSSFSDYVRGGIVTQVKMPKKIAFKSLSSSMAEPEFLLTDFAKFDRPGQLHVGFQALHAFEKKHSRLPKPWNQADADELMTLAEEVNAAQTGSAKQEELDQAVIKKLACVAAGDLAPVNAFIGGLAAQEVMKACTGKFMPIMQWLYFDALECLPEPEEVILTEEECAPRNCRYDGQIAVFGSKLQELLAKQRYFLVGAGAIGCELLKNFAMMGLASGEGEVIVTDMDTIEKSNLNRQFLFRPWDVTKMKSETAAAAVKQMNPSVRITGHQNRVGPDTEKVYDDDFFECLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFITESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENALQYLTDSKFMERTLKLPGAQPLEVVESVYKSLVTDRPRNWDDCVTWARNHWQCQYNNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLEFSTNNDLHMDYILAAANLYALSYGLPSCNDRSALTKLLQDIKVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKTLLPSLEASSQFKLCPIEFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELLKIVQGHKKLESYKNGFMNLALPFFAFSEPIAAPKHKYYEIDWTLWDRFEVKGIQPNGEEMTLRQFLDYFKNEHKLEITMLSQGVSMLYSFFMPAAKLKERLELPMTEIVTKVSKKKLGKHVKALVFELCCNDDTEEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	XM_005162051.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162051.4

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAGCGT CGCGTGTCAG GATCAGAGAC GAAGACGGGA 
TCCCATTGCT CCTCCTCTAA CTCTGTCAGA ACTGAACTGT CACACACACC AGCTAACGGC 
ATGGCTAAGA ATGGAAATGA TGCTGAGATC GACGAGGGCC TGTACTCCAG ACAGCTGTAC 
GTGCTGGGTC ATGATGCTAT GAAGCGTATG CAGAGCTCTA ATGTTCTTAT CTCGGGACTG 
CGAGGTTTAG GGGTAGAGAT CGCTAAGAAT GTCATTCTGG GTGGAGTCAA AAGCGTCACC 
CTGCACGATC AGGGAGTGGC CGAGTGGAAG GACCTTTCAT CACAGTTTTA CCTGCGTGAG 
GAGGATCTTG GGAAGAACCG TGCTGATGTC AGTCAGCCTC GACTGGCAGA ACTGAACAGT 
TATGTGCCTG TAACCTCCTA CACTGGCACC CTCACCAATG AATACCTGAC CAAATTTCAG 
GTTGTTGTGC TCACCAACTC TAGTCTTGAT GAACAGACAC GTATCGGGGA ATTTTGCCAC 
AGTAATGGCA TCAAGTTGAT TGTGGCAGAC ACACGTGGAC TGTTTGGGCA ACTGTTCTGT 
GATTTTGGGG AGGAAATGAT AGTATTCGAC ACAAACGGAG AACAGCCATT GAGCGCCATG 
ATCTCAATGA TCACAAAAGA CAGCGCTGGT GTGGTTACGT GTCTGGATGA AGCACGGCAC 
GGATTTGAGA GCGGAGACTA TGTCACTTTC ACAGAAGTGC AGGGCATGAC GGAACTTAAT 
GGCTGTGATC CAGTAGAGAT CAAGACACTG GGTCCTTACA CTTTCAGCAT CTGTGACACC 
AGCTCATTCT CTGACTATGT GAGAGGTGGT ATTGTCACAC AAGTGAAGAT GCCCAAGAAG 
ATTGCCTTTA AATCCCTGTC CTCCTCCATG GCCGAACCAG AGTTCTTGCT GACTGATTTT 
GCCAAATTTG ATCGTCCAGG CCAACTCCAC GTGGGCTTTC AGGCACTGCA TGCATTTGAG 
AAGAAGCACA GTCGTCTGCC AAAGCCCTGG AATCAGGCAG ATGCGGATGA GCTTATGACT 
CTGGCTGAAG AGGTGAATGC TGCACAGACA GGATCTGCCA AGCAAGAGGA GCTGGACCAG 
GCAGTCATAA AAAAGCTGGC CTGCGTGGCT GCTGGAGACC TGGCACCCGT CAATGCCTTT 
ATTGGTGGGC TTGCTGCCCA GGAGGTTATG AAGGCCTGCA CAGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTATTTTGA TGCTTTGGAG TGCTTACCTG AGCCTGAGGA AGTCATTCTT 
ACAGAGGAAG AGTGTGCTCC TAGGAACTGC CGGTATGACG GCCAGATTGC CGTCTTTGGG 
TCCAAGCTGC AGGAGCTGTT AGCCAAGCAG AGATATTTTC TGGTTGGTGC TGGAGCCATT 
GGCTGTGAGC TGCTGAAGAA CTTTGCTATG ATGGGCTTGG CCAGTGGTGA AGGAGAGGTC 
ATAGTCACTG ACATGGACAC CATTGAGAAG TCCAATCTCA ATCGACAGTT CCTCTTCAGA 
CCCTGGGATG TCACGAAGAT GAAGAGTGAG ACTGCTGCTG CGGCAGTGAA GCAGATGAAC 
CCGTCAGTCC GTATCACTGG TCACCAGAAC CGTGTGGGAC CGGACACGGA GAAGGTCTAT 
GATGATGACT TTTTTGAATG CCTTGATGGA GTGGCAAATG CTCTTGACAA TGTAGATGCC 
CGTATGTATA TGGATCGGAG GTGTGTGTAC TACCGTAAGC CATTGCTGGA GTCTGGTACT 
CTTGGCACCA AAGGAAACGT GCAGGTGGTC ATTCCCTTCA TCACAGAGTC CTACAGCTCC 
AGCCAGGATC CTCCAGAGAA GTCCATTCCT ATCTGCACAC TCAAGAACTT CCCAAATGCA 
ATCGAACACA CCCTACAGTG GGCTCGTGAT GAGTTTGAGG GACTGTTCAA GCAACCTGCT 
GAAAACGCCT TGCAGTATCT GACAGACTCA AAGTTTATGG AGCGCACACT GAAGCTCCCT 
GGAGCTCAGC CGCTGGAGGT GGTGGAATCG GTTTACAAGA GTCTGGTGAC AGACAGGCCA 
CGCAACTGGG ATGACTGTGT AACCTGGGCC CGCAACCACT GGCAGTGTCA ATATAACAAT 
AACATCCGTC AGCTGCTGCA CAATTTCCCC CCAGACCAGC TTACAAGCTC TGGGGCGCCA 
TTCTGGTCAG GTCCAAAGAG ATGTCCTCAT CCATTAGAAT TCAGCACTAA CAATGATCTT 
CACATGGACT ACATTTTGGC AGCAGCTAAC TTGTATGCCC TGTCATACGG CCTGCCATCC 
TGCAATGACC GCTCAGCTCT GACCAAGCTT TTGCAGGATA TTAAAGTGCC TGAGTTCACA 
CCCAAGTCAG GAGTCAAGAT TCACGTGTCT GATCAGGAGC TGCAGAGTGC CAACGCATCA 
GTCGATGACT CGAGGCTCGA AGAGCTGAAG ACACTGTTGC CTTCTTTAGA AGCATCTTCT 
CAATTCAAGC TCTGTCCTAT TGAATTTGAA AAGGACGATG ACACAAACTT CCACATGGAC 
TTCATTGTAG CGGCATCTAA TCTGAGAGCA GAGAACTATG ACATTCCTCC TGCAGACAGA 
CACAAGAGCA AACTCATTGC TGGAAAAATC ATTCCAGCCA TTGCCACAAC AACAGCCGCT 
GTTGTAGGTC TGGTGTGTCT GGAGTTGCTG AAGATCGTTC AGGGGCACAA GAAGCTCGAG 
TCCTACAAAA ATGGCTTCAT GAACCTTGCT CTGCCTTTCT TTGCCTTCTC CGAGCCTATA 
GCTGCCCCCA AGCACAAGTA CTACGAGATT GATTGGACAC TTTGGGACCG TTTTGAGGTG 
AAGGGCATTC AGCCTAATGG AGAGGAAATG ACACTCCGGC AGTTCCTGGA TTATTTCAAG 
AATGAACACA AACTGGAGAT CACGATGCTC TCTCAGGGAG TTTCTATGCT CTACTCTTTC 
TTCATGCCTG CAGCCAAACT CAAAGAAAGA CTGGAACTAC CGATGACTGA GATCGTGACC 
AAAGTGTCCA AGAAGAAACT GGGCAAGCAT GTCAAAGCAC TAGTGTTTGA GCTCTGCTGC 
AATGACGACA CAGAAGAAGA CGTAGAGGTG CCCTATGTCA GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uba1 ( XM_005162051.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162108.1 Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27570
Clone ID Related Accession (Same CDS sequence)	XM_005162051.4 , XM_005162051.3 , XM_005162052.3 , XM_005162052.4
Accession Version	XM_005162051.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin-like modifier-activating enzyme 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162051.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT CGCCGCTGTC CAAGAAGCGT CGCGTGTCAG GATCAGAGAC GAAGACGGGA 
TCCCATTGCT CCTCCTCTAA CTCTGTCAGA ACTGAACTGT CACACACACC AGCTAACGGC 
ATGGCTAAGA ATGGAAATGA TGCTGAGATC GACGAGGGCC TGTACTCCAG ACAGCTGTAC 
GTGCTGGGTC ATGATGCTAT GAAGCGTATG CAGAGCTCTA ATGTTCTTAT CTCGGGACTG 
CGAGGTTTAG GGGTAGAGAT CGCTAAGAAT GTCATTCTGG GTGGAGTCAA AAGCGTCACC 
CTGCACGATC AGGGAGTGGC CGAGTGGAAG GACCTTTCAT CACAGTTTTA CCTGCGTGAG 
GAGGATCTTG GGAAGAACCG TGCTGATGTC AGTCAGCCTC GACTGGCAGA ACTGAACAGT 
TATGTGCCTG TAACCTCCTA CACTGGCACC CTCACCAATG AATACCTGAC CAAATTTCAG 
GTTGTTGTGC TCACCAACTC TAGTCTTGAT GAACAGACAC GTATCGGGGA ATTTTGCCAC 
AGTAATGGCA TCAAGTTGAT TGTGGCAGAC ACACGTGGAC TGTTTGGGCA ACTGTTCTGT 
GATTTTGGGG AGGAAATGAT AGTATTCGAC ACAAACGGAG AACAGCCATT GAGCGCCATG 
ATCTCAATGA TCACAAAAGA CAGCGCTGGT GTGGTTACGT GTCTGGATGA AGCACGGCAC 
GGATTTGAGA GCGGAGACTA TGTCACTTTC ACAGAAGTGC AGGGCATGAC GGAACTTAAT 
GGCTGTGATC CAGTAGAGAT CAAGACACTG GGTCCTTACA CTTTCAGCAT CTGTGACACC 
AGCTCATTCT CTGACTATGT GAGAGGTGGT ATTGTCACAC AAGTGAAGAT GCCCAAGAAG 
ATTGCCTTTA AATCCCTGTC CTCCTCCATG GCCGAACCAG AGTTCTTGCT GACTGATTTT 
GCCAAATTTG ATCGTCCAGG CCAACTCCAC GTGGGCTTTC AGGCACTGCA TGCATTTGAG 
AAGAAGCACA GTCGTCTGCC AAAGCCCTGG AATCAGGCAG ATGCGGATGA GCTTATGACT 
CTGGCTGAAG AGGTGAATGC TGCACAGACA GGATCTGCCA AGCAAGAGGA GCTGGACCAG 
GCAGTCATAA AAAAGCTGGC CTGCGTGGCT GCTGGAGACC TGGCACCCGT CAATGCCTTT 
ATTGGTGGGC TTGCTGCCCA GGAGGTTATG AAGGCCTGCA CAGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTATTTTGA TGCTTTGGAG TGCTTACCTG AGCCTGAGGA AGTCATTCTT 
ACAGAGGAAG AGTGTGCTCC TAGGAACTGC CGGTATGACG GCCAGATTGC CGTCTTTGGG 
TCCAAGCTGC AGGAGCTGTT AGCCAAGCAG AGATATTTTC TGGTTGGTGC TGGAGCCATT 
GGCTGTGAGC TGCTGAAGAA CTTTGCTATG ATGGGCTTGG CCAGTGGTGA AGGAGAGGTC 
ATAGTCACTG ACATGGACAC CATTGAGAAG TCCAATCTCA ATCGACAGTT CCTCTTCAGA 
CCCTGGGATG TCACGAAGAT GAAGAGTGAG ACTGCTGCTG CGGCAGTGAA GCAGATGAAC 
CCGTCAGTCC GTATCACTGG TCACCAGAAC CGTGTGGGAC CGGACACGGA GAAGGTCTAT 
GATGATGACT TTTTTGAATG CCTTGATGGA GTGGCAAATG CTCTTGACAA TGTAGATGCC 
CGTATGTATA TGGATCGGAG GTGTGTGTAC TACCGTAAGC CATTGCTGGA GTCTGGTACT 
CTTGGCACCA AAGGAAACGT GCAGGTGGTC ATTCCCTTCA TCACAGAGTC CTACAGCTCC 
AGCCAGGATC CTCCAGAGAA GTCCATTCCT ATCTGCACAC TCAAGAACTT CCCAAATGCA 
ATCGAACACA CCCTACAGTG GGCTCGTGAT GAGTTTGAGG GACTGTTCAA GCAACCTGCT 
GAAAACGCCT TGCAGTATCT GACAGACTCA AAGTTTATGG AGCGCACACT GAAGCTCCCT 
GGAGCTCAGC CGCTGGAGGT GGTGGAATCG GTTTACAAGA GTCTGGTGAC AGACAGGCCA 
CGCAACTGGG ATGACTGTGT AACCTGGGCC CGCAACCACT GGCAGTGTCA ATATAACAAT 
AACATCCGTC AGCTGCTGCA CAATTTCCCC CCAGACCAGC TTACAAGCTC TGGGGCGCCA 
TTCTGGTCAG GTCCAAAGAG ATGTCCTCAT CCATTAGAAT TCAGCACTAA CAATGATCTT 
CACATGGACT ACATTTTGGC AGCAGCTAAC TTGTATGCCC TGTCATACGG CCTGCCATCC 
TGCAATGACC GCTCAGCTCT GACCAAGCTT TTGCAGGATA TTAAAGTGCC TGAGTTCACA 
CCCAAGTCAG GAGTCAAGAT TCACGTGTCT GATCAGGAGC TGCAGAGTGC CAACGCATCA 
GTCGATGACT CGAGGCTCGA AGAGCTGAAG ACACTGTTGC CTTCTTTAGA AGCATCTTCT 
CAATTCAAGC TCTGTCCTAT TGAATTTGAA AAGGACGATG ACACAAACTT CCACATGGAC 
TTCATTGTAG CGGCATCTAA TCTGAGAGCA GAGAACTATG ACATTCCTCC TGCAGACAGA 
CACAAGAGCA AACTCATTGC TGGAAAAATC ATTCCAGCCA TTGCCACAAC AACAGCCGCT 
GTTGTAGGTC TGGTGTGTCT GGAGTTGCTG AAGATCGTTC AGGGGCACAA GAAGCTCGAG 
TCCTACAAAA ATGGCTTCAT GAACCTTGCT CTGCCTTTCT TTGCCTTCTC CGAGCCTATA 
GCTGCCCCCA AGCACAAGTA CTACGAGATT GATTGGACAC TTTGGGACCG TTTTGAGGTG 
AAGGGCATTC AGCCTAATGG AGAGGAAATG ACACTCCGGC AGTTCCTGGA TTATTTCAAG 
AATGAACACA AACTGGAGAT CACGATGCTC TCTCAGGGAG TTTCTATGCT CTACTCTTTC 
TTCATGCCTG CAGCCAAACT CAAAGAAAGA CTGGAACTAC CGATGACTGA GATCGTGACC 
AAAGTGTCCA AGAAGAAACT GGGCAAGCAT GTCAAAGCAC TAGTGTTTGA GCTCTGCTGC 
AATGACGACA CAGAAGAAGA CGTAGAGGTG CCCTATGTCA GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162108.1
CDS	281..3457
Translation	MSSSPLSKKRRVSGSETKTGSHCSSSNSVRTELSHTPANGMAKNGNDAEIDEGLYSRQLYVLGHDAMKRMQSSNVLISGLRGLGVEIAKNVILGGVKSVTLHDQGVAEWKDLSSQFYLREEDLGKNRADVSQPRLAELNSYVPVTSYTGTLTNEYLTKFQVVVLTNSSLDEQTRIGEFCHSNGIKLIVADTRGLFGQLFCDFGEEMIVFDTNGEQPLSAMISMITKDSAGVVTCLDEARHGFESGDYVTFTEVQGMTELNGCDPVEIKTLGPYTFSICDTSSFSDYVRGGIVTQVKMPKKIAFKSLSSSMAEPEFLLTDFAKFDRPGQLHVGFQALHAFEKKHSRLPKPWNQADADELMTLAEEVNAAQTGSAKQEELDQAVIKKLACVAAGDLAPVNAFIGGLAAQEVMKACTGKFMPIMQWLYFDALECLPEPEEVILTEEECAPRNCRYDGQIAVFGSKLQELLAKQRYFLVGAGAIGCELLKNFAMMGLASGEGEVIVTDMDTIEKSNLNRQFLFRPWDVTKMKSETAAAAVKQMNPSVRITGHQNRVGPDTEKVYDDDFFECLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFITESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENALQYLTDSKFMERTLKLPGAQPLEVVESVYKSLVTDRPRNWDDCVTWARNHWQCQYNNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLEFSTNNDLHMDYILAAANLYALSYGLPSCNDRSALTKLLQDIKVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKTLLPSLEASSQFKLCPIEFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELLKIVQGHKKLESYKNGFMNLALPFFAFSEPIAAPKHKYYEIDWTLWDRFEVKGIQPNGEEMTLRQFLDYFKNEHKLEITMLSQGVSMLYSFFMPAAKLKERLELPMTEIVTKVSKKKLGKHVKALVFELCCNDDTEEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	XM_005162051.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162051.3

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAGCGT CGCGTGTCAG GATCAGAGAC GAAGACGGGA 
TCCCATTGCT CCTCCTCTAA CTCTGTCAGA ACTGAACTGT CACACACACC AGCTAACGGC 
ATGGCTAAGA ATGGAAATGA TGCTGAGATC GACGAGGGCC TGTACTCCAG ACAGCTGTAC 
GTGCTGGGTC ATGATGCTAT GAAGCGTATG CAGAGCTCTA ATGTTCTTAT CTCGGGACTG 
CGAGGTTTAG GGGTAGAGAT CGCTAAGAAT GTCATTCTGG GTGGAGTCAA AAGCGTCACC 
CTGCACGATC AGGGAGTGGC CGAGTGGAAG GACCTTTCAT CACAGTTTTA CCTGCGTGAG 
GAGGATCTTG GGAAGAACCG TGCTGATGTC AGTCAGCCTC GACTGGCAGA ACTGAACAGT 
TATGTGCCTG TAACCTCCTA CACTGGCACC CTCACCAATG AATACCTGAC CAAATTTCAG 
GTTGTTGTGC TCACCAACTC TAGTCTTGAT GAACAGACAC GTATCGGGGA ATTTTGCCAC 
AGTAATGGCA TCAAGTTGAT TGTGGCAGAC ACACGTGGAC TGTTTGGGCA ACTGTTCTGT 
GATTTTGGGG AGGAAATGAT AGTATTCGAC ACAAACGGAG AACAGCCATT GAGCGCCATG 
ATCTCAATGA TCACAAAAGA CAGCGCTGGT GTGGTTACGT GTCTGGATGA AGCACGGCAC 
GGATTTGAGA GCGGAGACTA TGTCACTTTC ACAGAAGTGC AGGGCATGAC GGAACTTAAT 
GGCTGTGATC CAGTAGAGAT CAAGACACTG GGTCCTTACA CTTTCAGCAT CTGTGACACC 
AGCTCATTCT CTGACTATGT GAGAGGTGGT ATTGTCACAC AAGTGAAGAT GCCCAAGAAG 
ATTGCCTTTA AATCCCTGTC CTCCTCCATG GCCGAACCAG AGTTCTTGCT GACTGATTTT 
GCCAAATTTG ATCGTCCAGG CCAACTCCAC GTGGGCTTTC AGGCACTGCA TGCATTTGAG 
AAGAAGCACA GTCGTCTGCC AAAGCCCTGG AATCAGGCAG ATGCGGATGA GCTTATGACT 
CTGGCTGAAG AGGTGAATGC TGCACAGACA GGATCTGCCA AGCAAGAGGA GCTGGACCAG 
GCAGTCATAA AAAAGCTGGC CTGCGTGGCT GCTGGAGACC TGGCACCCGT CAATGCCTTT 
ATTGGTGGGC TTGCTGCCCA GGAGGTTATG AAGGCCTGCA CAGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTATTTTGA TGCTTTGGAG TGCTTACCTG AGCCTGAGGA AGTCATTCTT 
ACAGAGGAAG AGTGTGCTCC TAGGAACTGC CGGTATGACG GCCAGATTGC CGTCTTTGGG 
TCCAAGCTGC AGGAGCTGTT AGCCAAGCAG AGATATTTTC TGGTTGGTGC TGGAGCCATT 
GGCTGTGAGC TGCTGAAGAA CTTTGCTATG ATGGGCTTGG CCAGTGGTGA AGGAGAGGTC 
ATAGTCACTG ACATGGACAC CATTGAGAAG TCCAATCTCA ATCGACAGTT CCTCTTCAGA 
CCCTGGGATG TCACGAAGAT GAAGAGTGAG ACTGCTGCTG CGGCAGTGAA GCAGATGAAC 
CCGTCAGTCC GTATCACTGG TCACCAGAAC CGTGTGGGAC CGGACACGGA GAAGGTCTAT 
GATGATGACT TTTTTGAATG CCTTGATGGA GTGGCAAATG CTCTTGACAA TGTAGATGCC 
CGTATGTATA TGGATCGGAG GTGTGTGTAC TACCGTAAGC CATTGCTGGA GTCTGGTACT 
CTTGGCACCA AAGGAAACGT GCAGGTGGTC ATTCCCTTCA TCACAGAGTC CTACAGCTCC 
AGCCAGGATC CTCCAGAGAA GTCCATTCCT ATCTGCACAC TCAAGAACTT CCCAAATGCA 
ATCGAACACA CCCTACAGTG GGCTCGTGAT GAGTTTGAGG GACTGTTCAA GCAACCTGCT 
GAAAACGCCT TGCAGTATCT GACAGACTCA AAGTTTATGG AGCGCACACT GAAGCTCCCT 
GGAGCTCAGC CGCTGGAGGT GGTGGAATCG GTTTACAAGA GTCTGGTGAC AGACAGGCCA 
CGCAACTGGG ATGACTGTGT AACCTGGGCC CGCAACCACT GGCAGTGTCA ATATAACAAT 
AACATCCGTC AGCTGCTGCA CAATTTCCCC CCAGACCAGC TTACAAGCTC TGGGGCGCCA 
TTCTGGTCAG GTCCAAAGAG ATGTCCTCAT CCATTAGAAT TCAGCACTAA CAATGATCTT 
CACATGGACT ACATTTTGGC AGCAGCTAAC TTGTATGCCC TGTCATACGG CCTGCCATCC 
TGCAATGACC GCTCAGCTCT GACCAAGCTT TTGCAGGATA TTAAAGTGCC TGAGTTCACA 
CCCAAGTCAG GAGTCAAGAT TCACGTGTCT GATCAGGAGC TGCAGAGTGC CAACGCATCA 
GTCGATGACT CGAGGCTCGA AGAGCTGAAG ACACTGTTGC CTTCTTTAGA AGCATCTTCT 
CAATTCAAGC TCTGTCCTAT TGAATTTGAA AAGGACGATG ACACAAACTT CCACATGGAC 
TTCATTGTAG CGGCATCTAA TCTGAGAGCA GAGAACTATG ACATTCCTCC TGCAGACAGA 
CACAAGAGCA AACTCATTGC TGGAAAAATC ATTCCAGCCA TTGCCACAAC AACAGCCGCT 
GTTGTAGGTC TGGTGTGTCT GGAGTTGCTG AAGATCGTTC AGGGGCACAA GAAGCTCGAG 
TCCTACAAAA ATGGCTTCAT GAACCTTGCT CTGCCTTTCT TTGCCTTCTC CGAGCCTATA 
GCTGCCCCCA AGCACAAGTA CTACGAGATT GATTGGACAC TTTGGGACCG TTTTGAGGTG 
AAGGGCATTC AGCCTAATGG AGAGGAAATG ACACTCCGGC AGTTCCTGGA TTATTTCAAG 
AATGAACACA AACTGGAGAT CACGATGCTC TCTCAGGGAG TTTCTATGCT CTACTCTTTC 
TTCATGCCTG CAGCCAAACT CAAAGAAAGA CTGGAACTAC CGATGACTGA GATCGTGACC 
AAAGTGTCCA AGAAGAAACT GGGCAAGCAT GTCAAAGCAC TAGTGTTTGA GCTCTGCTGC 
AATGACGACA CAGAAGAAGA CGTAGAGGTG CCCTATGTCA GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uba1 ( XM_005162052.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162109.1 Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27570
Clone ID Related Accession (Same CDS sequence)	XM_005162051.4 , XM_005162051.3 , XM_005162052.3 , XM_005162052.4
Accession Version	XM_005162052.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin-like modifier-activating enzyme 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162052.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT CGCCGCTGTC CAAGAAGCGT CGCGTGTCAG GATCAGAGAC GAAGACGGGA 
TCCCATTGCT CCTCCTCTAA CTCTGTCAGA ACTGAACTGT CACACACACC AGCTAACGGC 
ATGGCTAAGA ATGGAAATGA TGCTGAGATC GACGAGGGCC TGTACTCCAG ACAGCTGTAC 
GTGCTGGGTC ATGATGCTAT GAAGCGTATG CAGAGCTCTA ATGTTCTTAT CTCGGGACTG 
CGAGGTTTAG GGGTAGAGAT CGCTAAGAAT GTCATTCTGG GTGGAGTCAA AAGCGTCACC 
CTGCACGATC AGGGAGTGGC CGAGTGGAAG GACCTTTCAT CACAGTTTTA CCTGCGTGAG 
GAGGATCTTG GGAAGAACCG TGCTGATGTC AGTCAGCCTC GACTGGCAGA ACTGAACAGT 
TATGTGCCTG TAACCTCCTA CACTGGCACC CTCACCAATG AATACCTGAC CAAATTTCAG 
GTTGTTGTGC TCACCAACTC TAGTCTTGAT GAACAGACAC GTATCGGGGA ATTTTGCCAC 
AGTAATGGCA TCAAGTTGAT TGTGGCAGAC ACACGTGGAC TGTTTGGGCA ACTGTTCTGT 
GATTTTGGGG AGGAAATGAT AGTATTCGAC ACAAACGGAG AACAGCCATT GAGCGCCATG 
ATCTCAATGA TCACAAAAGA CAGCGCTGGT GTGGTTACGT GTCTGGATGA AGCACGGCAC 
GGATTTGAGA GCGGAGACTA TGTCACTTTC ACAGAAGTGC AGGGCATGAC GGAACTTAAT 
GGCTGTGATC CAGTAGAGAT CAAGACACTG GGTCCTTACA CTTTCAGCAT CTGTGACACC 
AGCTCATTCT CTGACTATGT GAGAGGTGGT ATTGTCACAC AAGTGAAGAT GCCCAAGAAG 
ATTGCCTTTA AATCCCTGTC CTCCTCCATG GCCGAACCAG AGTTCTTGCT GACTGATTTT 
GCCAAATTTG ATCGTCCAGG CCAACTCCAC GTGGGCTTTC AGGCACTGCA TGCATTTGAG 
AAGAAGCACA GTCGTCTGCC AAAGCCCTGG AATCAGGCAG ATGCGGATGA GCTTATGACT 
CTGGCTGAAG AGGTGAATGC TGCACAGACA GGATCTGCCA AGCAAGAGGA GCTGGACCAG 
GCAGTCATAA AAAAGCTGGC CTGCGTGGCT GCTGGAGACC TGGCACCCGT CAATGCCTTT 
ATTGGTGGGC TTGCTGCCCA GGAGGTTATG AAGGCCTGCA CAGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTATTTTGA TGCTTTGGAG TGCTTACCTG AGCCTGAGGA AGTCATTCTT 
ACAGAGGAAG AGTGTGCTCC TAGGAACTGC CGGTATGACG GCCAGATTGC CGTCTTTGGG 
TCCAAGCTGC AGGAGCTGTT AGCCAAGCAG AGATATTTTC TGGTTGGTGC TGGAGCCATT 
GGCTGTGAGC TGCTGAAGAA CTTTGCTATG ATGGGCTTGG CCAGTGGTGA AGGAGAGGTC 
ATAGTCACTG ACATGGACAC CATTGAGAAG TCCAATCTCA ATCGACAGTT CCTCTTCAGA 
CCCTGGGATG TCACGAAGAT GAAGAGTGAG ACTGCTGCTG CGGCAGTGAA GCAGATGAAC 
CCGTCAGTCC GTATCACTGG TCACCAGAAC CGTGTGGGAC CGGACACGGA GAAGGTCTAT 
GATGATGACT TTTTTGAATG CCTTGATGGA GTGGCAAATG CTCTTGACAA TGTAGATGCC 
CGTATGTATA TGGATCGGAG GTGTGTGTAC TACCGTAAGC CATTGCTGGA GTCTGGTACT 
CTTGGCACCA AAGGAAACGT GCAGGTGGTC ATTCCCTTCA TCACAGAGTC CTACAGCTCC 
AGCCAGGATC CTCCAGAGAA GTCCATTCCT ATCTGCACAC TCAAGAACTT CCCAAATGCA 
ATCGAACACA CCCTACAGTG GGCTCGTGAT GAGTTTGAGG GACTGTTCAA GCAACCTGCT 
GAAAACGCCT TGCAGTATCT GACAGACTCA AAGTTTATGG AGCGCACACT GAAGCTCCCT 
GGAGCTCAGC CGCTGGAGGT GGTGGAATCG GTTTACAAGA GTCTGGTGAC AGACAGGCCA 
CGCAACTGGG ATGACTGTGT AACCTGGGCC CGCAACCACT GGCAGTGTCA ATATAACAAT 
AACATCCGTC AGCTGCTGCA CAATTTCCCC CCAGACCAGC TTACAAGCTC TGGGGCGCCA 
TTCTGGTCAG GTCCAAAGAG ATGTCCTCAT CCATTAGAAT TCAGCACTAA CAATGATCTT 
CACATGGACT ACATTTTGGC AGCAGCTAAC TTGTATGCCC TGTCATACGG CCTGCCATCC 
TGCAATGACC GCTCAGCTCT GACCAAGCTT TTGCAGGATA TTAAAGTGCC TGAGTTCACA 
CCCAAGTCAG GAGTCAAGAT TCACGTGTCT GATCAGGAGC TGCAGAGTGC CAACGCATCA 
GTCGATGACT CGAGGCTCGA AGAGCTGAAG ACACTGTTGC CTTCTTTAGA AGCATCTTCT 
CAATTCAAGC TCTGTCCTAT TGAATTTGAA AAGGACGATG ACACAAACTT CCACATGGAC 
TTCATTGTAG CGGCATCTAA TCTGAGAGCA GAGAACTATG ACATTCCTCC TGCAGACAGA 
CACAAGAGCA AACTCATTGC TGGAAAAATC ATTCCAGCCA TTGCCACAAC AACAGCCGCT 
GTTGTAGGTC TGGTGTGTCT GGAGTTGCTG AAGATCGTTC AGGGGCACAA GAAGCTCGAG 
TCCTACAAAA ATGGCTTCAT GAACCTTGCT CTGCCTTTCT TTGCCTTCTC CGAGCCTATA 
GCTGCCCCCA AGCACAAGTA CTACGAGATT GATTGGACAC TTTGGGACCG TTTTGAGGTG 
AAGGGCATTC AGCCTAATGG AGAGGAAATG ACACTCCGGC AGTTCCTGGA TTATTTCAAG 
AATGAACACA AACTGGAGAT CACGATGCTC TCTCAGGGAG TTTCTATGCT CTACTCTTTC 
TTCATGCCTG CAGCCAAACT CAAAGAAAGA CTGGAACTAC CGATGACTGA GATCGTGACC 
AAAGTGTCCA AGAAGAAACT GGGCAAGCAT GTCAAAGCAC TAGTGTTTGA GCTCTGCTGC 
AATGACGACA CAGAAGAAGA CGTAGAGGTG CCCTATGTCA GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162109.1
CDS	237..3413
Translation	MSSSPLSKKRRVSGSETKTGSHCSSSNSVRTELSHTPANGMAKNGNDAEIDEGLYSRQLYVLGHDAMKRMQSSNVLISGLRGLGVEIAKNVILGGVKSVTLHDQGVAEWKDLSSQFYLREEDLGKNRADVSQPRLAELNSYVPVTSYTGTLTNEYLTKFQVVVLTNSSLDEQTRIGEFCHSNGIKLIVADTRGLFGQLFCDFGEEMIVFDTNGEQPLSAMISMITKDSAGVVTCLDEARHGFESGDYVTFTEVQGMTELNGCDPVEIKTLGPYTFSICDTSSFSDYVRGGIVTQVKMPKKIAFKSLSSSMAEPEFLLTDFAKFDRPGQLHVGFQALHAFEKKHSRLPKPWNQADADELMTLAEEVNAAQTGSAKQEELDQAVIKKLACVAAGDLAPVNAFIGGLAAQEVMKACTGKFMPIMQWLYFDALECLPEPEEVILTEEECAPRNCRYDGQIAVFGSKLQELLAKQRYFLVGAGAIGCELLKNFAMMGLASGEGEVIVTDMDTIEKSNLNRQFLFRPWDVTKMKSETAAAAVKQMNPSVRITGHQNRVGPDTEKVYDDDFFECLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFITESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENALQYLTDSKFMERTLKLPGAQPLEVVESVYKSLVTDRPRNWDDCVTWARNHWQCQYNNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLEFSTNNDLHMDYILAAANLYALSYGLPSCNDRSALTKLLQDIKVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKTLLPSLEASSQFKLCPIEFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELLKIVQGHKKLESYKNGFMNLALPFFAFSEPIAAPKHKYYEIDWTLWDRFEVKGIQPNGEEMTLRQFLDYFKNEHKLEITMLSQGVSMLYSFFMPAAKLKERLELPMTEIVTKVSKKKLGKHVKALVFELCCNDDTEEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	XM_005162052.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162052.3

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAGCGT CGCGTGTCAG GATCAGAGAC GAAGACGGGA 
TCCCATTGCT CCTCCTCTAA CTCTGTCAGA ACTGAACTGT CACACACACC AGCTAACGGC 
ATGGCTAAGA ATGGAAATGA TGCTGAGATC GACGAGGGCC TGTACTCCAG ACAGCTGTAC 
GTGCTGGGTC ATGATGCTAT GAAGCGTATG CAGAGCTCTA ATGTTCTTAT CTCGGGACTG 
CGAGGTTTAG GGGTAGAGAT CGCTAAGAAT GTCATTCTGG GTGGAGTCAA AAGCGTCACC 
CTGCACGATC AGGGAGTGGC CGAGTGGAAG GACCTTTCAT CACAGTTTTA CCTGCGTGAG 
GAGGATCTTG GGAAGAACCG TGCTGATGTC AGTCAGCCTC GACTGGCAGA ACTGAACAGT 
TATGTGCCTG TAACCTCCTA CACTGGCACC CTCACCAATG AATACCTGAC CAAATTTCAG 
GTTGTTGTGC TCACCAACTC TAGTCTTGAT GAACAGACAC GTATCGGGGA ATTTTGCCAC 
AGTAATGGCA TCAAGTTGAT TGTGGCAGAC ACACGTGGAC TGTTTGGGCA ACTGTTCTGT 
GATTTTGGGG AGGAAATGAT AGTATTCGAC ACAAACGGAG AACAGCCATT GAGCGCCATG 
ATCTCAATGA TCACAAAAGA CAGCGCTGGT GTGGTTACGT GTCTGGATGA AGCACGGCAC 
GGATTTGAGA GCGGAGACTA TGTCACTTTC ACAGAAGTGC AGGGCATGAC GGAACTTAAT 
GGCTGTGATC CAGTAGAGAT CAAGACACTG GGTCCTTACA CTTTCAGCAT CTGTGACACC 
AGCTCATTCT CTGACTATGT GAGAGGTGGT ATTGTCACAC AAGTGAAGAT GCCCAAGAAG 
ATTGCCTTTA AATCCCTGTC CTCCTCCATG GCCGAACCAG AGTTCTTGCT GACTGATTTT 
GCCAAATTTG ATCGTCCAGG CCAACTCCAC GTGGGCTTTC AGGCACTGCA TGCATTTGAG 
AAGAAGCACA GTCGTCTGCC AAAGCCCTGG AATCAGGCAG ATGCGGATGA GCTTATGACT 
CTGGCTGAAG AGGTGAATGC TGCACAGACA GGATCTGCCA AGCAAGAGGA GCTGGACCAG 
GCAGTCATAA AAAAGCTGGC CTGCGTGGCT GCTGGAGACC TGGCACCCGT CAATGCCTTT 
ATTGGTGGGC TTGCTGCCCA GGAGGTTATG AAGGCCTGCA CAGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTATTTTGA TGCTTTGGAG TGCTTACCTG AGCCTGAGGA AGTCATTCTT 
ACAGAGGAAG AGTGTGCTCC TAGGAACTGC CGGTATGACG GCCAGATTGC CGTCTTTGGG 
TCCAAGCTGC AGGAGCTGTT AGCCAAGCAG AGATATTTTC TGGTTGGTGC TGGAGCCATT 
GGCTGTGAGC TGCTGAAGAA CTTTGCTATG ATGGGCTTGG CCAGTGGTGA AGGAGAGGTC 
ATAGTCACTG ACATGGACAC CATTGAGAAG TCCAATCTCA ATCGACAGTT CCTCTTCAGA 
CCCTGGGATG TCACGAAGAT GAAGAGTGAG ACTGCTGCTG CGGCAGTGAA GCAGATGAAC 
CCGTCAGTCC GTATCACTGG TCACCAGAAC CGTGTGGGAC CGGACACGGA GAAGGTCTAT 
GATGATGACT TTTTTGAATG CCTTGATGGA GTGGCAAATG CTCTTGACAA TGTAGATGCC 
CGTATGTATA TGGATCGGAG GTGTGTGTAC TACCGTAAGC CATTGCTGGA GTCTGGTACT 
CTTGGCACCA AAGGAAACGT GCAGGTGGTC ATTCCCTTCA TCACAGAGTC CTACAGCTCC 
AGCCAGGATC CTCCAGAGAA GTCCATTCCT ATCTGCACAC TCAAGAACTT CCCAAATGCA 
ATCGAACACA CCCTACAGTG GGCTCGTGAT GAGTTTGAGG GACTGTTCAA GCAACCTGCT 
GAAAACGCCT TGCAGTATCT GACAGACTCA AAGTTTATGG AGCGCACACT GAAGCTCCCT 
GGAGCTCAGC CGCTGGAGGT GGTGGAATCG GTTTACAAGA GTCTGGTGAC AGACAGGCCA 
CGCAACTGGG ATGACTGTGT AACCTGGGCC CGCAACCACT GGCAGTGTCA ATATAACAAT 
AACATCCGTC AGCTGCTGCA CAATTTCCCC CCAGACCAGC TTACAAGCTC TGGGGCGCCA 
TTCTGGTCAG GTCCAAAGAG ATGTCCTCAT CCATTAGAAT TCAGCACTAA CAATGATCTT 
CACATGGACT ACATTTTGGC AGCAGCTAAC TTGTATGCCC TGTCATACGG CCTGCCATCC 
TGCAATGACC GCTCAGCTCT GACCAAGCTT TTGCAGGATA TTAAAGTGCC TGAGTTCACA 
CCCAAGTCAG GAGTCAAGAT TCACGTGTCT GATCAGGAGC TGCAGAGTGC CAACGCATCA 
GTCGATGACT CGAGGCTCGA AGAGCTGAAG ACACTGTTGC CTTCTTTAGA AGCATCTTCT 
CAATTCAAGC TCTGTCCTAT TGAATTTGAA AAGGACGATG ACACAAACTT CCACATGGAC 
TTCATTGTAG CGGCATCTAA TCTGAGAGCA GAGAACTATG ACATTCCTCC TGCAGACAGA 
CACAAGAGCA AACTCATTGC TGGAAAAATC ATTCCAGCCA TTGCCACAAC AACAGCCGCT 
GTTGTAGGTC TGGTGTGTCT GGAGTTGCTG AAGATCGTTC AGGGGCACAA GAAGCTCGAG 
TCCTACAAAA ATGGCTTCAT GAACCTTGCT CTGCCTTTCT TTGCCTTCTC CGAGCCTATA 
GCTGCCCCCA AGCACAAGTA CTACGAGATT GATTGGACAC TTTGGGACCG TTTTGAGGTG 
AAGGGCATTC AGCCTAATGG AGAGGAAATG ACACTCCGGC AGTTCCTGGA TTATTTCAAG 
AATGAACACA AACTGGAGAT CACGATGCTC TCTCAGGGAG TTTCTATGCT CTACTCTTTC 
TTCATGCCTG CAGCCAAACT CAAAGAAAGA CTGGAACTAC CGATGACTGA GATCGTGACC 
AAAGTGTCCA AGAAGAAACT GGGCAAGCAT GTCAAAGCAC TAGTGTTTGA GCTCTGCTGC 
AATGACGACA CAGAAGAAGA CGTAGAGGTG CCCTATGTCA GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uba1 ( XM_005162052.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162109.1 Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), transcript variant X2, mRNA.

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CloneID	ODa27570
Clone ID Related Accession (Same CDS sequence)	XM_005162051.4 , XM_005162051.3 , XM_005162052.3 , XM_005162052.4
Accession Version	XM_005162052.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin-like modifier-activating enzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162052.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT CGCCGCTGTC CAAGAAGCGT CGCGTGTCAG GATCAGAGAC GAAGACGGGA 
TCCCATTGCT CCTCCTCTAA CTCTGTCAGA ACTGAACTGT CACACACACC AGCTAACGGC 
ATGGCTAAGA ATGGAAATGA TGCTGAGATC GACGAGGGCC TGTACTCCAG ACAGCTGTAC 
GTGCTGGGTC ATGATGCTAT GAAGCGTATG CAGAGCTCTA ATGTTCTTAT CTCGGGACTG 
CGAGGTTTAG GGGTAGAGAT CGCTAAGAAT GTCATTCTGG GTGGAGTCAA AAGCGTCACC 
CTGCACGATC AGGGAGTGGC CGAGTGGAAG GACCTTTCAT CACAGTTTTA CCTGCGTGAG 
GAGGATCTTG GGAAGAACCG TGCTGATGTC AGTCAGCCTC GACTGGCAGA ACTGAACAGT 
TATGTGCCTG TAACCTCCTA CACTGGCACC CTCACCAATG AATACCTGAC CAAATTTCAG 
GTTGTTGTGC TCACCAACTC TAGTCTTGAT GAACAGACAC GTATCGGGGA ATTTTGCCAC 
AGTAATGGCA TCAAGTTGAT TGTGGCAGAC ACACGTGGAC TGTTTGGGCA ACTGTTCTGT 
GATTTTGGGG AGGAAATGAT AGTATTCGAC ACAAACGGAG AACAGCCATT GAGCGCCATG 
ATCTCAATGA TCACAAAAGA CAGCGCTGGT GTGGTTACGT GTCTGGATGA AGCACGGCAC 
GGATTTGAGA GCGGAGACTA TGTCACTTTC ACAGAAGTGC AGGGCATGAC GGAACTTAAT 
GGCTGTGATC CAGTAGAGAT CAAGACACTG GGTCCTTACA CTTTCAGCAT CTGTGACACC 
AGCTCATTCT CTGACTATGT GAGAGGTGGT ATTGTCACAC AAGTGAAGAT GCCCAAGAAG 
ATTGCCTTTA AATCCCTGTC CTCCTCCATG GCCGAACCAG AGTTCTTGCT GACTGATTTT 
GCCAAATTTG ATCGTCCAGG CCAACTCCAC GTGGGCTTTC AGGCACTGCA TGCATTTGAG 
AAGAAGCACA GTCGTCTGCC AAAGCCCTGG AATCAGGCAG ATGCGGATGA GCTTATGACT 
CTGGCTGAAG AGGTGAATGC TGCACAGACA GGATCTGCCA AGCAAGAGGA GCTGGACCAG 
GCAGTCATAA AAAAGCTGGC CTGCGTGGCT GCTGGAGACC TGGCACCCGT CAATGCCTTT 
ATTGGTGGGC TTGCTGCCCA GGAGGTTATG AAGGCCTGCA CAGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTATTTTGA TGCTTTGGAG TGCTTACCTG AGCCTGAGGA AGTCATTCTT 
ACAGAGGAAG AGTGTGCTCC TAGGAACTGC CGGTATGACG GCCAGATTGC CGTCTTTGGG 
TCCAAGCTGC AGGAGCTGTT AGCCAAGCAG AGATATTTTC TGGTTGGTGC TGGAGCCATT 
GGCTGTGAGC TGCTGAAGAA CTTTGCTATG ATGGGCTTGG CCAGTGGTGA AGGAGAGGTC 
ATAGTCACTG ACATGGACAC CATTGAGAAG TCCAATCTCA ATCGACAGTT CCTCTTCAGA 
CCCTGGGATG TCACGAAGAT GAAGAGTGAG ACTGCTGCTG CGGCAGTGAA GCAGATGAAC 
CCGTCAGTCC GTATCACTGG TCACCAGAAC CGTGTGGGAC CGGACACGGA GAAGGTCTAT 
GATGATGACT TTTTTGAATG CCTTGATGGA GTGGCAAATG CTCTTGACAA TGTAGATGCC 
CGTATGTATA TGGATCGGAG GTGTGTGTAC TACCGTAAGC CATTGCTGGA GTCTGGTACT 
CTTGGCACCA AAGGAAACGT GCAGGTGGTC ATTCCCTTCA TCACAGAGTC CTACAGCTCC 
AGCCAGGATC CTCCAGAGAA GTCCATTCCT ATCTGCACAC TCAAGAACTT CCCAAATGCA 
ATCGAACACA CCCTACAGTG GGCTCGTGAT GAGTTTGAGG GACTGTTCAA GCAACCTGCT 
GAAAACGCCT TGCAGTATCT GACAGACTCA AAGTTTATGG AGCGCACACT GAAGCTCCCT 
GGAGCTCAGC CGCTGGAGGT GGTGGAATCG GTTTACAAGA GTCTGGTGAC AGACAGGCCA 
CGCAACTGGG ATGACTGTGT AACCTGGGCC CGCAACCACT GGCAGTGTCA ATATAACAAT 
AACATCCGTC AGCTGCTGCA CAATTTCCCC CCAGACCAGC TTACAAGCTC TGGGGCGCCA 
TTCTGGTCAG GTCCAAAGAG ATGTCCTCAT CCATTAGAAT TCAGCACTAA CAATGATCTT 
CACATGGACT ACATTTTGGC AGCAGCTAAC TTGTATGCCC TGTCATACGG CCTGCCATCC 
TGCAATGACC GCTCAGCTCT GACCAAGCTT TTGCAGGATA TTAAAGTGCC TGAGTTCACA 
CCCAAGTCAG GAGTCAAGAT TCACGTGTCT GATCAGGAGC TGCAGAGTGC CAACGCATCA 
GTCGATGACT CGAGGCTCGA AGAGCTGAAG ACACTGTTGC CTTCTTTAGA AGCATCTTCT 
CAATTCAAGC TCTGTCCTAT TGAATTTGAA AAGGACGATG ACACAAACTT CCACATGGAC 
TTCATTGTAG CGGCATCTAA TCTGAGAGCA GAGAACTATG ACATTCCTCC TGCAGACAGA 
CACAAGAGCA AACTCATTGC TGGAAAAATC ATTCCAGCCA TTGCCACAAC AACAGCCGCT 
GTTGTAGGTC TGGTGTGTCT GGAGTTGCTG AAGATCGTTC AGGGGCACAA GAAGCTCGAG 
TCCTACAAAA ATGGCTTCAT GAACCTTGCT CTGCCTTTCT TTGCCTTCTC CGAGCCTATA 
GCTGCCCCCA AGCACAAGTA CTACGAGATT GATTGGACAC TTTGGGACCG TTTTGAGGTG 
AAGGGCATTC AGCCTAATGG AGAGGAAATG ACACTCCGGC AGTTCCTGGA TTATTTCAAG 
AATGAACACA AACTGGAGAT CACGATGCTC TCTCAGGGAG TTTCTATGCT CTACTCTTTC 
TTCATGCCTG CAGCCAAACT CAAAGAAAGA CTGGAACTAC CGATGACTGA GATCGTGACC 
AAAGTGTCCA AGAAGAAACT GGGCAAGCAT GTCAAAGCAC TAGTGTTTGA GCTCTGCTGC 
AATGACGACA CAGAAGAAGA CGTAGAGGTG CCCTATGTCA GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162109.1
CDS	237..3413
Translation	MSSSPLSKKRRVSGSETKTGSHCSSSNSVRTELSHTPANGMAKNGNDAEIDEGLYSRQLYVLGHDAMKRMQSSNVLISGLRGLGVEIAKNVILGGVKSVTLHDQGVAEWKDLSSQFYLREEDLGKNRADVSQPRLAELNSYVPVTSYTGTLTNEYLTKFQVVVLTNSSLDEQTRIGEFCHSNGIKLIVADTRGLFGQLFCDFGEEMIVFDTNGEQPLSAMISMITKDSAGVVTCLDEARHGFESGDYVTFTEVQGMTELNGCDPVEIKTLGPYTFSICDTSSFSDYVRGGIVTQVKMPKKIAFKSLSSSMAEPEFLLTDFAKFDRPGQLHVGFQALHAFEKKHSRLPKPWNQADADELMTLAEEVNAAQTGSAKQEELDQAVIKKLACVAAGDLAPVNAFIGGLAAQEVMKACTGKFMPIMQWLYFDALECLPEPEEVILTEEECAPRNCRYDGQIAVFGSKLQELLAKQRYFLVGAGAIGCELLKNFAMMGLASGEGEVIVTDMDTIEKSNLNRQFLFRPWDVTKMKSETAAAAVKQMNPSVRITGHQNRVGPDTEKVYDDDFFECLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFITESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENALQYLTDSKFMERTLKLPGAQPLEVVESVYKSLVTDRPRNWDDCVTWARNHWQCQYNNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLEFSTNNDLHMDYILAAANLYALSYGLPSCNDRSALTKLLQDIKVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKTLLPSLEASSQFKLCPIEFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELLKIVQGHKKLESYKNGFMNLALPFFAFSEPIAAPKHKYYEIDWTLWDRFEVKGIQPNGEEMTLRQFLDYFKNEHKLEITMLSQGVSMLYSFFMPAAKLKERLELPMTEIVTKVSKKKLGKHVKALVFELCCNDDTEEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	XM_005162052.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin-like modifier activating enzyme 1 (uba1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162052.4

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAGCGT CGCGTGTCAG GATCAGAGAC GAAGACGGGA 
TCCCATTGCT CCTCCTCTAA CTCTGTCAGA ACTGAACTGT CACACACACC AGCTAACGGC 
ATGGCTAAGA ATGGAAATGA TGCTGAGATC GACGAGGGCC TGTACTCCAG ACAGCTGTAC 
GTGCTGGGTC ATGATGCTAT GAAGCGTATG CAGAGCTCTA ATGTTCTTAT CTCGGGACTG 
CGAGGTTTAG GGGTAGAGAT CGCTAAGAAT GTCATTCTGG GTGGAGTCAA AAGCGTCACC 
CTGCACGATC AGGGAGTGGC CGAGTGGAAG GACCTTTCAT CACAGTTTTA CCTGCGTGAG 
GAGGATCTTG GGAAGAACCG TGCTGATGTC AGTCAGCCTC GACTGGCAGA ACTGAACAGT 
TATGTGCCTG TAACCTCCTA CACTGGCACC CTCACCAATG AATACCTGAC CAAATTTCAG 
GTTGTTGTGC TCACCAACTC TAGTCTTGAT GAACAGACAC GTATCGGGGA ATTTTGCCAC 
AGTAATGGCA TCAAGTTGAT TGTGGCAGAC ACACGTGGAC TGTTTGGGCA ACTGTTCTGT 
GATTTTGGGG AGGAAATGAT AGTATTCGAC ACAAACGGAG AACAGCCATT GAGCGCCATG 
ATCTCAATGA TCACAAAAGA CAGCGCTGGT GTGGTTACGT GTCTGGATGA AGCACGGCAC 
GGATTTGAGA GCGGAGACTA TGTCACTTTC ACAGAAGTGC AGGGCATGAC GGAACTTAAT 
GGCTGTGATC CAGTAGAGAT CAAGACACTG GGTCCTTACA CTTTCAGCAT CTGTGACACC 
AGCTCATTCT CTGACTATGT GAGAGGTGGT ATTGTCACAC AAGTGAAGAT GCCCAAGAAG 
ATTGCCTTTA AATCCCTGTC CTCCTCCATG GCCGAACCAG AGTTCTTGCT GACTGATTTT 
GCCAAATTTG ATCGTCCAGG CCAACTCCAC GTGGGCTTTC AGGCACTGCA TGCATTTGAG 
AAGAAGCACA GTCGTCTGCC AAAGCCCTGG AATCAGGCAG ATGCGGATGA GCTTATGACT 
CTGGCTGAAG AGGTGAATGC TGCACAGACA GGATCTGCCA AGCAAGAGGA GCTGGACCAG 
GCAGTCATAA AAAAGCTGGC CTGCGTGGCT GCTGGAGACC TGGCACCCGT CAATGCCTTT 
ATTGGTGGGC TTGCTGCCCA GGAGGTTATG AAGGCCTGCA CAGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTATTTTGA TGCTTTGGAG TGCTTACCTG AGCCTGAGGA AGTCATTCTT 
ACAGAGGAAG AGTGTGCTCC TAGGAACTGC CGGTATGACG GCCAGATTGC CGTCTTTGGG 
TCCAAGCTGC AGGAGCTGTT AGCCAAGCAG AGATATTTTC TGGTTGGTGC TGGAGCCATT 
GGCTGTGAGC TGCTGAAGAA CTTTGCTATG ATGGGCTTGG CCAGTGGTGA AGGAGAGGTC 
ATAGTCACTG ACATGGACAC CATTGAGAAG TCCAATCTCA ATCGACAGTT CCTCTTCAGA 
CCCTGGGATG TCACGAAGAT GAAGAGTGAG ACTGCTGCTG CGGCAGTGAA GCAGATGAAC 
CCGTCAGTCC GTATCACTGG TCACCAGAAC CGTGTGGGAC CGGACACGGA GAAGGTCTAT 
GATGATGACT TTTTTGAATG CCTTGATGGA GTGGCAAATG CTCTTGACAA TGTAGATGCC 
CGTATGTATA TGGATCGGAG GTGTGTGTAC TACCGTAAGC CATTGCTGGA GTCTGGTACT 
CTTGGCACCA AAGGAAACGT GCAGGTGGTC ATTCCCTTCA TCACAGAGTC CTACAGCTCC 
AGCCAGGATC CTCCAGAGAA GTCCATTCCT ATCTGCACAC TCAAGAACTT CCCAAATGCA 
ATCGAACACA CCCTACAGTG GGCTCGTGAT GAGTTTGAGG GACTGTTCAA GCAACCTGCT 
GAAAACGCCT TGCAGTATCT GACAGACTCA AAGTTTATGG AGCGCACACT GAAGCTCCCT 
GGAGCTCAGC CGCTGGAGGT GGTGGAATCG GTTTACAAGA GTCTGGTGAC AGACAGGCCA 
CGCAACTGGG ATGACTGTGT AACCTGGGCC CGCAACCACT GGCAGTGTCA ATATAACAAT 
AACATCCGTC AGCTGCTGCA CAATTTCCCC CCAGACCAGC TTACAAGCTC TGGGGCGCCA 
TTCTGGTCAG GTCCAAAGAG ATGTCCTCAT CCATTAGAAT TCAGCACTAA CAATGATCTT 
CACATGGACT ACATTTTGGC AGCAGCTAAC TTGTATGCCC TGTCATACGG CCTGCCATCC 
TGCAATGACC GCTCAGCTCT GACCAAGCTT TTGCAGGATA TTAAAGTGCC TGAGTTCACA 
CCCAAGTCAG GAGTCAAGAT TCACGTGTCT GATCAGGAGC TGCAGAGTGC CAACGCATCA 
GTCGATGACT CGAGGCTCGA AGAGCTGAAG ACACTGTTGC CTTCTTTAGA AGCATCTTCT 
CAATTCAAGC TCTGTCCTAT TGAATTTGAA AAGGACGATG ACACAAACTT CCACATGGAC 
TTCATTGTAG CGGCATCTAA TCTGAGAGCA GAGAACTATG ACATTCCTCC TGCAGACAGA 
CACAAGAGCA AACTCATTGC TGGAAAAATC ATTCCAGCCA TTGCCACAAC AACAGCCGCT 
GTTGTAGGTC TGGTGTGTCT GGAGTTGCTG AAGATCGTTC AGGGGCACAA GAAGCTCGAG 
TCCTACAAAA ATGGCTTCAT GAACCTTGCT CTGCCTTTCT TTGCCTTCTC CGAGCCTATA 
GCTGCCCCCA AGCACAAGTA CTACGAGATT GATTGGACAC TTTGGGACCG TTTTGAGGTG 
AAGGGCATTC AGCCTAATGG AGAGGAAATG ACACTCCGGC AGTTCCTGGA TTATTTCAAG 
AATGAACACA AACTGGAGAT CACGATGCTC TCTCAGGGAG TTTCTATGCT CTACTCTTTC 
TTCATGCCTG CAGCCAAACT CAAAGAAAGA CTGGAACTAC CGATGACTGA GATCGTGACC 
AAAGTGTCCA AGAAGAAACT GGGCAAGCAT GTCAAAGCAC TAGTGTTTGA GCTCTGCTGC 
AATGACGACA CAGAAGAAGA CGTAGAGGTG CCCTATGTCA GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Dysregulation of ubiquitin homeostasis and ?-catenin signaling promote spinal muscular atrophy.
The Journal of clinical investigation124(4)1821-34(2014 Apr)
Wishart TM,Mutsaers CA,Riessland M,Reimer MM,Hunter G,Hannam ML,Eaton SL,Fuller HR,Roche SL,Somers E,Morse R,Young PJ,Lamont DJ,Hammerschmidt M,Joshi A,Hohenstein P,Morris GE,Parson SH,Skehel PA,Becker T,Robinson IM,Becker CG,Wirth B,Gillingwater TH

Global identification of SMAD2 target genes reveals a role for multiple co-regulatory factors in zebrafish early gastrulas.
The Journal of biological chemistry286(32)28520-32(2011 Aug)
Liu Z,Lin X,Cai Z,Zhang Z,Han C,Jia S,Meng A,Wang Q

Transcriptome kinetics of arsenic-induced adaptive response in zebrafish liver.
Physiological genomics27(3)351-61(2006 Nov)
Lam SH,Winata CL,Tong Y,Korzh S,Lim WS,Korzh V,Spitsbergen J,Mathavan S,Miller LD,Liu ET,Gong Z

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uba1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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