cacna1da cDNA ORF clone, Danio rerio(zebrafish)

The following cacna1da gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1da cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa36068 NM_203484.1
Latest version!
Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa147856 XM_021479576.1
Latest version!
Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa147857 XM_021479577.1
Latest version!
Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa147858 XM_021479578.1
Latest version!
Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa147859 XM_021479579.1
Latest version!
Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa147860 XM_021479580.1
Latest version!
Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa147861 XM_021479581.1
Latest version!
Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa147862 XM_021479582.1
Latest version!
Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa147863 XM_021479583.1
Latest version!
Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa147864 XM_021479584.1
Latest version!
Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa36068
    Clone ID Related Accession (Same CDS sequence) NM_203484.1
    Accession Version NM_203484.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent L-type calcium channel subunit alpha-1D
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY528224.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY528224.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAATGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCGGG TTACACTTGC CAGCCTCATG GAGAAGAAGA AGAAGGGGTT TGGCTGGTTC
    AGCCAGTCCT CTGACACTCA TGCGAGTATG CCGGCAAGTG AGACTGAATC CATGAATACA
    GAGAACGAGA AAGGAGAAGA TGAGAAGGCG ACATGTTGCG GCCCGACATG TCAAAAAATA
    TCAAAATCAA AGTTCAGTCG GCGCTGGCGT CGCTGGAACC GGTTGTGTCG GAGGAATTGT
    CGCTTGGCTG TAAAATCTGT GCCATTCTAT TGGCTGGTAA TCATCCTGGT GTTCCTGAAC
    ACTTTGACCA TCTCTTCAGA ACATTACAAC CAGCCCATGT GGCTCACACA AGTACAGGAT
    GTGGCTAATA AAGTGCTGCT GGCCATGTTC ACGTGTGAGA TGTTGGTGAA GATGTACAGT
    CTGGGCCTGC AGGCTTATTT TGTGTCTCTT TTTAATCGCT TTGACTGCTT TGTGGTGTGT
    GGAGGCATCA CTGAAACCAT CCTTGTGGAG TTTGAGATTA TGTCACCCCT CGGCATCTCA
    GTGTTTCGAT GTGTTCGACT TCTCAGGATC TTTAAAGTTA CACGCCACTG GGCGTCTCTC
    AGTAACCTGG TGGCATCTCT GCTGAACTCC ATGAAGTCCA TTGCTTCTCT ACTGCTGCTG
    CTCTTCCTCT TCATCATCAT CTTTAGCCTC CTCGGTATGC AGGTGTTCGG CGGCAAGTTT
    AACTTTGACG AGACACAAAC CAAGAGGAGC ACCTTTGACA ACTTCCCTCA GGCCTTGCTC
    ACTGTGTTTC AGATTCTAAC TGGTGAAGAC TGGAATGCCG TCATGTATGA CGGAATTATG
    GCGTACGGCG GCCCTTCGTC ATCAGGAATG ATTGTGTGCA TATACTTCAT CATCCTTTTC
    ATCTGTGGAA ACTACATTCT CCTGAATGTG TTCTTGGCTA TCGCTGTGGA CAATCTTGCT
    GATGCAGAGT CTCTGAACAC GGACGATACG AAGAAACCTG ATGAAATCGA CGAAATTGAA
    GATGAAGCCA AGGCTGGAGA GGAGGATGAG AAGGATAATG CCGAGGAGGA TGAAGAAGAG
    CCAGATGTTC CTGCAGGGCC TCGTCCAAAG ATCTCAGAGC TTGTTAAGAA GGAGAAGATC
    ACTCCAATCC CAGAGGGCAG TGCGTTCTTC ATCTTCAGCA ACACAAACCC AGTTCGAGTC
    GCCTGCCACA AGCTGATAAA CCACCACATC TTCACCAACC TCATCCTTGT GTTCATTATG
    CTAAGCTCAG CGTCATTGGC TGCTGAGGAT CCAATCAGAA ACTTCTCTGC TAGGAATATT
    ATACTTGGTT ACTTTGACTA TGCTTTCACG GCTATCTTTA CTGTAGAGAT CGTGTTAAAG
    ATGACCACGT ATGGCGCTTT TCTCCATAAG GGTGCTTTCT GCAGGAACTA CTTTAATCTT
    CTAGATCTGC TGGTGGTCGG AGTTTCGCTC GTGTCCTTCG GGATTCAATC ATCGGCCATC
    TCAGTGGTGA AGATTCTGAG GGTTCTGAGA GTTTTGCGTC CACTCAGAGC CATAAACAGA
    GCTAAAGGAC TAAAGCATGT GGTCCAGTGT GTGTTTGTGG CCATCAGGAC TATAGGAAAC
    ATCATGATCG TCACCACTCT GCTGCAGTTT ATGTTCGCCT GCATTGGTGT CCAGCTCTTC
    AAGGGAAAGT TTTACCGTTG TAACGATGAG GCCAAGTCCA GCCCAGAGGA GTGCAAGGGA
    ACGTACATCC TGTATAAGGA AGGAGACGTG AACCAGCCAA TCATTCAGAA GCGCCACTGG
    CACAACAGTG ATTTCAACTT TGACAATGTG CTCATGGCCA TGATGGCTCT GTTCACCGTG
    TCCACGTTTG AAGGCTGGCC AGCGTTGCTG TACAAGGCCA TCGACTCCAA CCGGGAGAAC
    ATGGGTCCCA TCTACAACTA CCGAGTGGAG ATTTCCATCT TCTTCATCAT CTACATCATC
    ATCATCGCTT TCTTTATGAT GAACATCTTT GTGGGTTTCG TCATCGTGAC CTTTCAGGAA
    CAGGGAGAGA AGGAGTACAA GAACTGTGAG CTGGACAAGA ACCAGCGTCA GTGTGTGGAG
    TATGCATTGA AAGCACGTCC TCTCAGGAGG TACATCCCAA AGAACCCGTA TCAGTATAAG
    TTCTGGTATG TGGTGAACTC CACCGGCTTC GAGTACATCA TGTTTGTCCT GATTTTACTC
    AACACCATCT GTCTCGCCGT GCAGCACTAT GGTCAGTCTG AGCTCTTCAA TTATGTTATG
    GACATTCTGA ATATGGTCTT CACTGCCGTC TTCACTGTGG AGATGGTGCT CAAGCTGATC
    GCCTTCAAGC CTCGGCATTA TTTCACTGAC GCATGGAACA CATTTGACGC CTTAATTGTT
    GTCGGTAGCG TCGTAGATAT TGCTATCACT GAAGTCAACA ACACTGAGGA CAGCGCTCGC
    ATTTCCATCA CTTTCTTCCG TCTTTTTCGA GTGATGCGAT TGGTCAAGCT GCTGAGCAGA
    GGGGAGGGRA TTCGAACCCT ACTCTGGACC TTTATCAAAT CCTTCCAGGC AYTGCCTTAT
    GTTGCTTTGC TGATAGCCAT GCTCTTCTTC ATCTACGCTG TCATTGGCAT GCAGGTGTTT
    GGGAAAATTG CCATGGTGGA TCACACTCAA ATCAACAGGA ACAACAACTT TCAGACGTTC
    CCTCAGGCTG TGCTTCTGCT CTTCAGGTGT GCTACGGGTG AAGCCTGGCA GGAGATCATG
    TTAGCATGTA TGCCAGGCAA GCTGTGTGAC CCGGAGTCAG ACTATAATCC TGGGGAAGAA
    ATGACCTGCG GGAGCAGTTT CGCCATCATC TACTTCATCA CCTTCTACAT GCTCTGCGCC
    TTCYTGATCA TTAACCTGTT TGTGGCTGTC ATCATGGACA ACTTCGACTA TCTGACCCGT
    GATTGGTCAR TTCTGGGGCC ACATCACCTA GATGAGTTTA AGAGGATTTG GTCAGAATAT
    GACCCAGAGG CCAAGGGCAG AATAAAACAT CTTGATGTAG TGACGTTGTT GCGCCGTATT
    CAGCCTCCTC TCGGCTTTGG GAAACTCTGT CCTCATAGAG TCGCCTGCAA GAGGCTGGTG
    GCTATGAACA TGCCCCTGAA CAGTGACGGC ACGGTCATGT TTAACGCGAC RCTGTTTGCT
    CTCGTGAGAA CCGCAKTGAA AATCAAAACA GAAGGTAACC TAGAACAAGC GAATGAAGAG
    CTCCGAGCTG TGATTAAGAA GATCTGGAAG AGAACCAGCA TGAAGCTTCT GGATCAAGTG
    GTTCCCCCTG CTGGTGATGA TGAGGTAACG GTGGGGAAGT TCTATGCCAC TTTCCTGATA
    CAGGACTACT TTAGGAAATT CAAGAAACGC AAAGAGGAAG GCCTGGTGGG AGTTCATCCG
    GCACAGAACA ACACGGCCAT CGCACTGCAG GCTGGCCTTC GTACGCTGCA TGACATTGGG
    CCAGAAATCC GCCGTGCGAT ATCATGTGAC CTGCAGGATG ATGAGCTAGT AGACTTTATA
    CCAGAGGAGG ACGAAGAAAT TTATAGGCGA AACGGAGGAC TCTTTGGCAA CCACATCAAC
    CACATAAACG GAGACCCCCG GAGGAGCTCT GGCCACCAAA CAAACGCCAC ACAGCGCCCC
    CTGCAGGTGC AGCCGCCCCC ACATTACGTC CACATGGAGC AGCCCGTGGG GCGTCTGGGT
    CGCGCCAATG CCATGGCACA GCAAAACCAT CATCGTCACC ACCACCACCA TCACCATCAT
    CATCATCACA ACAACTCCTA CAACAAATCG CCCAAGTCCA CCAACATCAA CCTCAACAAC
    GCCAACGTGT CCAGTTTNCC CAATGGTGGA CACAATCGCT ACTACGAACA CGCTCCCGCT
    AATGGCTACC CAGGGTCATA TTACGGAGAA TACGACAAGC CCAGGACCCC TCATGGGCAA
    AGAAGGCGCT ACTATGAGAC ATATATTAGA TCGCAGGGCA GCGACAGAAG ACGACCCACG
    ATACGCCGCG AAGAGGAGTA TGAAGAAGAT CGCTACTCTG GAGAGTATTA CAGCGGAGAG
    GAGTTTTATG AAGATGACAG CATGCTATCG GGTGATAGGT ACCCCAACAG TGACCAGGAG
    TACGAGACGC CGCGGGGTTA CCATCATCCT GACAGTTACT ATGAAGATGA TGAGCAGCCC
    CTTTATCACG ATTCACATAG GTCACCAAAG AGAAGACTGC TTCCTCCAAC ACCGCAAGGT
    AATCGGAGAC CGTCGTTCAA TTTTGAGTGT CTGCGCAGAC AGAGCAGTCA AGACGATCTT
    CCTCACCAGC GAACCGCTCT TCCGTTACAT CTCATGCAAC ACCAGGTGAT GGCAGTGGCG
    GGTCTGGACT CTAGCAGGGC TCACAGGCTT TCACCAACTC GATCCACCAG ATCATGGGCT
    TCACCTCCTC CAACTCCTGC AAGTAAAGAC CGGACGCCGT ACTACACACC GCTCATCCGA
    GTGGACCGTC CTCTAAGAGA CAGCGCCAGC AGCAGCCATT CATCCATCAG GAAGAGCTCG
    TGGTACACAG ACGACCCTGA GTACCAGCAG AGGAACTTNT CACCTGTTCA CCTGCAGGTC
    CCACCCGAGT ACAGGAACCA GTACCTGCAG AAGAGAGGCA GCGCTACCAG CCTGGTGGAG
    GCGGTCTTGA TATCAGAAGG ACTGGGTCGA TATGCCAAGG ATCCCAAATT TGTGGCCGCC
    NCAAAGCATG AGATTGCGGA CGCGTGCGAG ATGACCATTG ACGAAATGGA AAGCGCCGCC
    AGCCATNTTT TGAACGGTGG CATTACACCA GTAGTCAACG GCGTCAATGT GTTTCCCATA
    CTGGGCCACA GAGAATACGA GCTGCAGGAC GTCTCGGCGA GCTACAGCGA CGAGGAGCCC
    GAACCCGAGC CCAGACCTCG TTACGAGGAG GACCTGGCAG ATGAAATGAT ATGCATTACC
    ACTTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_982351.1
    CDS223..6471
    Translation

    Target ORF information:

    RefSeq Version NM_203484.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_203484.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAATGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCGGG TTACACTTGC CAGCCTCATG GAGAAGAAGA AGAAGGGGTT TGGCTGGTTC
    AGCCAGTCCT CTGACACTCA TGCGAGTATG CCGGCAAGTG AGACTGAATC CATGAATACA
    GAGAACGAGA AAGGAGAAGA TGAGAAGGCG ACATGTTGCG GCCCGACATG TCAAAAAATA
    TCAAAATCAA AGTTCAGTCG GCGCTGGCGT CGCTGGAACC GGTTGTGTCG GAGGAATTGT
    CGCTTGGCTG TAAAATCTGT GCCATTCTAT TGGCTGGTAA TCATCCTGGT GTTCCTGAAC
    ACTTTGACCA TCTCTTCAGA ACATTACAAC CAGCCCATGT GGCTCACACA AGTACAGGAT
    GTGGCTAATA AAGTGCTGCT GGCCATGTTC ACGTGTGAGA TGTTGGTGAA GATGTACAGT
    CTGGGCCTGC AGGCTTATTT TGTGTCTCTT TTTAATCGCT TTGACTGCTT TGTGGTGTGT
    GGAGGCATCA CTGAAACCAT CCTTGTGGAG TTTGAGATTA TGTCACCCCT CGGCATCTCA
    GTGTTTCGAT GTGTTCGACT TCTCAGGATC TTTAAAGTTA CACGCCACTG GGCGTCTCTC
    AGTAACCTGG TGGCATCTCT GCTGAACTCC ATGAAGTCCA TTGCTTCTCT ACTGCTGCTG
    CTCTTCCTCT TCATCATCAT CTTTAGCCTC CTCGGTATGC AGGTGTTCGG CGGCAAGTTT
    AACTTTGACG AGACACAAAC CAAGAGGAGC ACCTTTGACA ACTTCCCTCA GGCCTTGCTC
    ACTGTGTTTC AGATTCTAAC TGGTGAAGAC TGGAATGCCG TCATGTATGA CGGAATTATG
    GCGTACGGCG GCCCTTCGTC ATCAGGAATG ATTGTGTGCA TATACTTCAT CATCCTTTTC
    ATCTGTGGAA ACTACATTCT CCTGAATGTG TTCTTGGCTA TCGCTGTGGA CAATCTTGCT
    GATGCAGAGT CTCTGAACAC GGACGATACG AAGAAACCTG ATGAAATCGA CGAAATTGAA
    GATGAAGCCA AGGCTGGAGA GGAGGATGAG AAGGATAATG CCGAGGAGGA TGAAGAAGAG
    CCAGATGTTC CTGCAGGGCC TCGTCCAAAG ATCTCAGAGC TTGTTAAGAA GGAGAAGATC
    ACTCCAATCC CAGAGGGCAG TGCGTTCTTC ATCTTCAGCA ACACAAACCC AGTTCGAGTC
    GCCTGCCACA AGCTGATAAA CCACCACATC TTCACCAACC TCATCCTTGT GTTCATTATG
    CTAAGCTCAG CGTCATTGGC TGCTGAGGAT CCAATCAGAA ACTTCTCTGC TAGGAATATT
    ATACTTGGTT ACTTTGACTA TGCTTTCACG GCTATCTTTA CTGTAGAGAT CGTGTTAAAG
    ATGACCACGT ATGGCGCTTT TCTCCATAAG GGTGCTTTCT GCAGGAACTA CTTTAATCTT
    CTAGATCTGC TGGTGGTCGG AGTTTCGCTC GTGTCCTTCG GGATTCAATC ATCGGCCATC
    TCAGTGGTGA AGATTCTGAG GGTTCTGAGA GTTTTGCGTC CACTCAGAGC CATAAACAGA
    GCTAAAGGAC TAAAGCATGT GGTCCAGTGT GTGTTTGTGG CCATCAGGAC TATAGGAAAC
    ATCATGATCG TCACCACTCT GCTGCAGTTT ATGTTCGCCT GCATTGGTGT CCAGCTCTTC
    AAGGGAAAGT TTTACCGTTG TAACGATGAG GCCAAGTCCA GCCCAGAGGA GTGCAAGGGA
    ACGTACATCC TGTATAAGGA AGGAGACGTG AACCAGCCAA TCATTCAGAA GCGCCACTGG
    CACAACAGTG ATTTCAACTT TGACAATGTG CTCATGGCCA TGATGGCTCT GTTCACCGTG
    TCCACGTTTG AAGGCTGGCC AGCGTTGCTG TACAAGGCCA TCGACTCCAA CCGGGAGAAC
    ATGGGTCCCA TCTACAACTA CCGAGTGGAG ATTTCCATCT TCTTCATCAT CTACATCATC
    ATCATCGCTT TCTTTATGAT GAACATCTTT GTGGGTTTCG TCATCGTGAC CTTTCAGGAA
    CAGGGAGAGA AGGAGTACAA GAACTGTGAG CTGGACAAGA ACCAGCGTCA GTGTGTGGAG
    TATGCATTGA AAGCACGTCC TCTCAGGAGG TACATCCCAA AGAACCCGTA TCAGTATAAG
    TTCTGGTATG TGGTGAACTC CACCGGCTTC GAGTACATCA TGTTTGTCCT GATTTTACTC
    AACACCATCT GTCTCGCCGT GCAGCACTAT GGTCAGTCTG AGCTCTTCAA TTATGTTATG
    GACATTCTGA ATATGGTCTT CACTGCCGTC TTCACTGTGG AGATGGTGCT CAAGCTGATC
    GCCTTCAAGC CTCGGCATTA TTTCACTGAC GCATGGAACA CATTTGACGC CTTAATTGTT
    GTCGGTAGCG TCGTAGATAT TGCTATCACT GAAGTCAACA ACACTGAGGA CAGCGCTCGC
    ATTTCCATCA CTTTCTTCCG TCTTTTTCGA GTGATGCGAT TGGTCAAGCT GCTGAGCAGA
    GGGGAGGGRA TTCGAACCCT ACTCTGGACC TTTATCAAAT CCTTCCAGGC AYTGCCTTAT
    GTTGCTTTGC TGATAGCCAT GCTCTTCTTC ATCTACGCTG TCATTGGCAT GCAGGTGTTT
    GGGAAAATTG CCATGGTGGA TCACACTCAA ATCAACAGGA ACAACAACTT TCAGACGTTC
    CCTCAGGCTG TGCTTCTGCT CTTCAGGTGT GCTACGGGTG AAGCCTGGCA GGAGATCATG
    TTAGCATGTA TGCCAGGCAA GCTGTGTGAC CCGGAGTCAG ACTATAATCC TGGGGAAGAA
    ATGACCTGCG GGAGCAGTTT CGCCATCATC TACTTCATCA CCTTCTACAT GCTCTGCGCC
    TTCYTGATCA TTAACCTGTT TGTGGCTGTC ATCATGGACA ACTTCGACTA TCTGACCCGT
    GATTGGTCAR TTCTGGGGCC ACATCACCTA GATGAGTTTA AGAGGATTTG GTCAGAATAT
    GACCCAGAGG CCAAGGGCAG AATAAAACAT CTTGATGTAG TGACGTTGTT GCGCCGTATT
    CAGCCTCCTC TCGGCTTTGG GAAACTCTGT CCTCATAGAG TCGCCTGCAA GAGGCTGGTG
    GCTATGAACA TGCCCCTGAA CAGTGACGGC ACGGTCATGT TTAACGCGAC RCTGTTTGCT
    CTCGTGAGAA CCGCAKTGAA AATCAAAACA GAAGGTAACC TAGAACAAGC GAATGAAGAG
    CTCCGAGCTG TGATTAAGAA GATCTGGAAG AGAACCAGCA TGAAGCTTCT GGATCAAGTG
    GTTCCCCCTG CTGGTGATGA TGAGGTAACG GTGGGGAAGT TCTATGCCAC TTTCCTGATA
    CAGGACTACT TTAGGAAATT CAAGAAACGC AAAGAGGAAG GCCTGGTGGG AGTTCATCCG
    GCACAGAACA ACACGGCCAT CGCACTGCAG GCTGGCCTTC GTACGCTGCA TGACATTGGG
    CCAGAAATCC GCCGTGCGAT ATCATGTGAC CTGCAGGATG ATGAGCTAGT AGACTTTATA
    CCAGAGGAGG ACGAAGAAAT TTATAGGCGA AACGGAGGAC TCTTTGGCAA CCACATCAAC
    CACATAAACG GAGACCCCCG GAGGAGCTCT GGCCACCAAA CAAACGCCAC ACAGCGCCCC
    CTGCAGGTGC AGCCGCCCCC ACATTACGTC CACATGGAGC AGCCCGTGGG GCGTCTGGGT
    CGCGCCAATG CCATGGCACA GCAAAACCAT CATCGTCACC ACCACCACCA TCACCATCAT
    CATCATCACA ACAACTCCTA CAACAAATCG CCCAAGTCCA CCAACATCAA CCTCAACAAC
    GCCAACGTGT CCAGTTTNCC CAATGGTGGA CACAATCGCT ACTACGAACA CGCTCCCGCT
    AATGGCTACC CAGGGTCATA TTACGGAGAA TACGACAAGC CCAGGACCCC TCATGGGCAA
    AGAAGGCGCT ACTATGAGAC ATATATTAGA TCGCAGGGCA GCGACAGAAG ACGACCCACG
    ATACGCCGCG AAGAGGAGTA TGAAGAAGAT CGCTACTCTG GAGAGTATTA CAGCGGAGAG
    GAGTTTTATG AAGATGACAG CATGCTATCG GGTGATAGGT ACCCCAACAG TGACCAGGAG
    TACGAGACGC CGCGGGGTTA CCATCATCCT GACAGTTACT ATGAAGATGA TGAGCAGCCC
    CTTTATCACG ATTCACATAG GTCACCAAAG AGAAGACTGC TTCCTCCAAC ACCGCAAGGT
    AATCGGAGAC CGTCGTTCAA TTTTGAGTGT CTGCGCAGAC AGAGCAGTCA AGACGATCTT
    CCTCACCAGC GAACCGCTCT TCCGTTACAT CTCATGCAAC ACCAGGTGAT GGCAGTGGCG
    GGTCTGGACT CTAGCAGGGC TCACAGGCTT TCACCAACTC GATCCACCAG ATCATGGGCT
    TCACCTCCTC CAACTCCTGC AAGTAAAGAC CGGACGCCGT ACTACACACC GCTCATCCGA
    GTGGACCGTC CTCTAAGAGA CAGCGCCAGC AGCAGCCATT CATCCATCAG GAAGAGCTCG
    TGGTACACAG ACGACCCTGA GTACCAGCAG AGGAACTTNT CACCTGTTCA CCTGCAGGTC
    CCACCCGAGT ACAGGAACCA GTACCTGCAG AAGAGAGGCA GCGCTACCAG CCTGGTGGAG
    GCGGTCTTGA TATCAGAAGG ACTGGGTCGA TATGCCAAGG ATCCCAAATT TGTGGCCGCC
    NCAAAGCATG AGATTGCGGA CGCGTGCGAG ATGACCATTG ACGAAATGGA AAGCGCCGCC
    AGCCATNTTT TGAACGGTGG CATTACACCA GTAGTCAACG GCGTCAATGT GTTTCCCATA
    CTGGGCCACA GAGAATACGA GCTGCAGGAC GTCTCGGCGA GCTACAGCGA CGAGGAGCCC
    GAACCCGAGC CCAGACCTCG TTACGAGGAG GACCTGGCAG ATGAAATGAT ATGCATTACC
    ACTTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147856
    Clone ID Related Accession (Same CDS sequence) XM_021479576.1
    Accession Version XM_021479576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCGGG TTACACTTGC CAGCCTCATG GAGAAGAAGA AGAAGGGGTT TGGCTGGTTC
    AGCCAGTCCT CTGACACTCA TGCGAGTATG CCGGCAAGTG AGACTGAATC CATGAATACA
    GAGAACGAGA AAGGAGAAGA TGAGAAGGCG ACATGTTGCG GCCCGACATG TCAAAAAATA
    TCAAAATCAA AGTTCAGTCG GCGCTGGCGT CGCTGGAACC GGTTGTGTCG GAGGAATTGT
    CGCTTGGCTG TAAAATCTGT GCCATTCTAT TGGCTGGTAA TCATCCTGGT GTTCCTGAAC
    ACTTTGACCA TCTCTTCAGA ACATTACAAC CAGCCCATGT GGCTCACACA AGTACAGGAT
    GTGGCTAATA AAGTGCTGCT GGCCATGTTC ACGTGTGAGA TGTTGGTGAA GATGTACAGT
    CTGGGCCTGC AGGCTTATTT TGTGTCTCTT TTTAATCGCT TTGACTGCTT TGTGGTGTGT
    GGAGGCATCA CTGAAACCAT CCTTGTGGAG TTTGAGATTA TGTCACCCCT CGGCATCTCA
    GTGTTTCGAT GTGTTCGACT TCTCAGGATC TTTAAAGTTA CACGCCACTG GGCGTCTCTC
    AGTAACCTGG TGGCATCTCT GCTGAACTCC ATGAAGTCCA TTGCTTCTCT ACTGCTGCTG
    CTCTTCCTCT TCATCATCAT CTTTAGCCTC CTCGGTATGC AGGTGTTCGG CGGCAAGTTT
    AACTTTGACG AGACACAAAC CAAGAGGAGC ACCTTTGACA ACTTCCCTCA GGCCTTGCTC
    ACTGTGTTTC AGATTCTAAC TGGTGAAGAC TGGAATGCCG TCATGTATGA CGGAATTATG
    GCGTACGGCG GCCCTTCGTC ATCAGGAATG ATTGTGTGCA TATACTTCAT CATCCTTTTC
    ATCTGTGGAA ACTACATTCT CCTGAATGTG TTCTTGGCTA TCGCTGTGGA CAATCTTGCT
    GATGCAGAGT CTCTGAACAC GGACGATACG AAGAAACCTG ATGAAATCGA CGAAATTGAA
    GATGAAGCCA AGGCTGGAGA GGAGGATGAG AAGGATAATG CCGAGGAGGA TGAAGAAGAG
    CCAGATGTTC CTGCAGGGCC TCGTCCAAAG ATCTCAGAGC TTGTTAAGAA GGAGAAGATC
    ACTCCAATCC CAGAGGGCAG TGCGTTCTTC ATCTTCAGCA ACACAAACCC AGTTCGAGTC
    GCCTGCCACA AGCTGATAAA CCACCACATC TTCACCAACC TCATCCTTGT GTTCATTATG
    CTAAGCTCAG CGTCATTGGC TGCTGAGGAT CCAATCAGAA ACTTCTCTGC TAGGAATATT
    ATACTTGGTT ACTTTGACTA TGCTTTCACG GCTATCTTTA CTGTAGAGAT CGTGTTAAAG
    ATGACCACGT ATGGCGCTTT TCTCCATAAG GGTGCTTTCT GCAGGAACTA CTTTAATCTT
    CTAGATCTGC TGGTGGTCGG AGTTTCGCTC GTGTCCTTCG GGATTCAATC ATCGGCCATC
    TCAGTGGTGA AGATTCTGAG GGTTCTGAGA GTTTTGCGTC CACTCAGAGC CATAAACAGA
    GCTAAAGGAC TAAAGCATGT GGTCCAGTGT GTGTTTGTGG CCATCAGGAC TATAGGAAAC
    ATCATGATCG TCACCACTCT GCTGCAGTTT ATGTTCGCCT GCATTGGTGT CCAGCTCTTC
    AAGGGAAAGT TTTACCGTTG TAACGATGAG GCCAAGTCCA GCCCAGAGGA GTGCAAGGGA
    ACGTACATCA TGTATAAGGA AGGAGACGTG AACCAGCCAA TCATTCAGAA GCGCCACTGG
    CACAACAGTG ATTTCAACTT TGACAATGTG CTCATGGCCA TGATGGCTCT GTTCACCGTG
    TCCACGTTTG AAGGCTGGCC AGCGTTGCTG TACAAGGCCA TCGACTCCAA CCGGGAGAAC
    ATGGGTCCCA TCTACAACTA CCGAGTGGAG ATTTCCATCT TCTTCATCAT CTACATCATC
    ATCATCGCTT TCTTTATGAT GAACATCTTT GTGGGTTTCG TCATCGTGAC CTTTCAGGAA
    CAGGGAGAGA AGGAGTACAA GAACTGTGAG CTGGACAAGA ACCAGCGTCA GTGTGTGGAG
    TATGCATTGA AAGCACGTCC TCTCAGGAGG TACATCCCAA AGAACCCATA TCAGTATAAG
    TTCTGGTATG TGGTGAACTC CACCGGCTTC GAGTACATCA TGTTTGTCCT GATTTTACTC
    AACACCATCT GTCTCGCCGT GCAGCACTAT GGTCAGTCTG AGCTCTTCAA TTATGTTATG
    GACATTCTGA ATATGGTCTT CACTGCCGTC TTCACTGTGG AGATGGTGCT CAAGCTGATC
    GCCTTCAAGC CTCGGCATTA TTTCACTGAC GCATGGAACA CATTTGACGC CTTAATTGTT
    GTCGGTAGCG TCGTAGATAT TGCTATCACT GAAGTCAACC CCACAGAGGC ACCTCAGGTG
    GATGAGTCGG GGAACACTGA GGACAGCGCT CGCATTTCCA TCACTTTCTT CCGTCTTTTT
    CGAGTGATGC GATTGGTCAA GCTGCTGAGC AGAGGGGAGG GAATTCGAAC CCTACTCTGG
    ACCTTTATCA AATCCTTCCA GGCACTGCCT TATGTTGCTT TGCTGATAGC CATGCTCTTC
    TTCATCTACG CTGTCATTGG CATGCAGGTG TTTGGGAAAA TTGCCATGGT GGATCACACT
    CAAATCAACA GGAACAACAA CTTTCAGACG TTCCCTCAGG CTGTGCTTCT GCTCTTCAGG
    TGTGCTACGG GTGAAGCCTG GCAGGAGATC ATGTTAGCAT GTATGCCAGG CAAGCTGTGT
    GACCCGGAGT CAGACTATAA TCCTGGGGAA GAAATGACCT GCGGGAGCAG TTTCGCCATC
    ATCTACTTCA TCACCTTCTA CATGCTCTGC GCCTTCCTGA TCATTAACCT GTTTGTGGCT
    GTCATCATGG ACAACTTCGA CTATCTGACC CGTGATTGGT CAATTCTGGG GCCACATCAC
    CTTGATGAGT TTAAGAGGAT TTGGTCAGAA TATGACCCAG AGGCCAAGGG CAGAATAAAA
    CATCTTGATG TAGTGACGTT GTTGCGCCGT ATTCAGCCTC CTCTCGGCTT TGGGAAACTC
    TGTCCTCATA GAGTCGCCTG CAAGAGGCTG GTGGCTATGA ACATGCCCCT GAACAGTGAC
    GGCACGGTCA TGTTTAACGC GACACTGTTT GCTCTCGTGA GAACCGCATT GAAAATCAAA
    ACAGAAGGTA ACCTAGAACA AGCGAATGAA GAGCTCCGAG CTGTGATTAA GAAGATCTGG
    AAGAGAACCA GCATGAAGCT TCTGGATCAA GTGGTTCCCC CTGCTGGTGA TGATGAGGTA
    ACGGTGGGGA AGTTCTATGC CACTTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA
    CGCAAAGAGG AAGGCCTGGT GGGAGTTCAT CCGGCACAGA ACAACACGGC CATCGCACTG
    CAGGCTGGCC TTCGTACGCT GCATGACATT GGGCCAGAAA TCCGCCGTGC GATATCATGT
    GACCTGCAGG ATGATGAGCT AGTAGACTTT ATACCAGAGG AGGACGAAGA AATTTATAGG
    CGAAACGGAG GACTCTTTGG CAACCACATC AACCACATAA ACGGAGACCC CCGGAGGAGC
    TCTGGCCACC AAACAAACGC CACACAGCGC CCCCTGCAGG TGCAGCCGCC CCCACATTAC
    GTCCACATGG AGCAGCCCGT GGGGCGTCTG GGTCGCGCCA ATGCCATGGC ACAGCAAAAC
    CATCATCGTC ACCACCACCA CCATCACCAT CATCATCATC ACAACAACTC CTACAACAAA
    TCGCCCAAGT CCACCAACAT CAACCTCAAC AACGCCAACG TGTCCAGTTT ACCCAATGGT
    GGACACAATC GCTACTACGA ACACGCTCCC GCTAATGGCT ACCCAGGGTC ATATTACGGA
    GAATACGACA AGCCCAGGAC CCCTCATGGG CAAAGAAGGC GCTACTATGA GACATATATT
    AGATCGCAGG GCAGCGACAG AAGACGACCC ACGATACGCC GCGAAGAGGA GTATGAAGAA
    GATCGCTACT CTGGAGAGTA TTACAGCGGA GAGGAGTTTT ATGAAGATGA CAGCATGCTA
    TCGGGTGATA GGTACCCCAA CAGTGACCAG GAGTACGAGA CGCCGCGGGG TTACCATCAT
    CCTGACAGTT ACTATGAAGA TGATGAGCAG CCCCTTTATC ACGATTCACA TAGGTCACCA
    AAGAGAAGAC TGCTTCCTCC AACACCGCAA GGTAATCGGA GACCGTCGTT CAATTTTGAG
    TGTCTGCGCA GACAGAGCAG TCAAGACGAT CTTCCTCACC AGCGAACTGC TCTTCCGTTA
    CATCTCATGC AACACCAGGT GATGGCAGTG GCGGGTCTGG ACTCTAGCAG GGCTCACAGG
    CTTTCACCAA CTCGATCTAC CAGATCATGG GCTTCACCTC CTCCAACTCC TGCAAGTAAA
    GACCGGACGC CGTACTACAC ACCGCTCATC CGCGTGGACC GTCCTCTAAG AGACAGCGCC
    AGCAGCAGCC ATTCATCCAT CAGGAAGAGC TCGTGGTACA CAGACGACCC TGAGTACCAG
    CAGAGGAACT TCTCACCTGT TCACCTGCAG GTCCCACCCG AGTACAGGAA CCAGTACCTG
    CAGAAGAGAG GCAGCGCTAC CAGCCTGGTG GAGGCGGTCT TGATATCAGA AGGACTGGGT
    CGATACGCCA AGGATCCCAA ATTTGTGGCC GCCACAAAGC ATGAGATTGC GGACGCGTGC
    GAGATGACCA TTGACGAAAT GGAAAGCGCC GCCAGCCATC TTCTTAACGG TGGCATTACA
    CCAGTAGTCA ACGGAGTCAA TGTGTTTCCC ATACTGGGCC ACAGAGACTA CGAGCTGCAG
    GACGTCTCGG CGAGCTACAG CGACGAGGAG CCCGAACCCG AGCCCAGACC TCGTTACGAG
    GAGGACCTTG CAGATGAAAT GATATGCATT ACCACTTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335251.1
    CDS411..6692
    Translation

    Target ORF information:

    RefSeq Version XM_021479576.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCGGG TTACACTTGC CAGCCTCATG GAGAAGAAGA AGAAGGGGTT TGGCTGGTTC
    AGCCAGTCCT CTGACACTCA TGCGAGTATG CCGGCAAGTG AGACTGAATC CATGAATACA
    GAGAACGAGA AAGGAGAAGA TGAGAAGGCG ACATGTTGCG GCCCGACATG TCAAAAAATA
    TCAAAATCAA AGTTCAGTCG GCGCTGGCGT CGCTGGAACC GGTTGTGTCG GAGGAATTGT
    CGCTTGGCTG TAAAATCTGT GCCATTCTAT TGGCTGGTAA TCATCCTGGT GTTCCTGAAC
    ACTTTGACCA TCTCTTCAGA ACATTACAAC CAGCCCATGT GGCTCACACA AGTACAGGAT
    GTGGCTAATA AAGTGCTGCT GGCCATGTTC ACGTGTGAGA TGTTGGTGAA GATGTACAGT
    CTGGGCCTGC AGGCTTATTT TGTGTCTCTT TTTAATCGCT TTGACTGCTT TGTGGTGTGT
    GGAGGCATCA CTGAAACCAT CCTTGTGGAG TTTGAGATTA TGTCACCCCT CGGCATCTCA
    GTGTTTCGAT GTGTTCGACT TCTCAGGATC TTTAAAGTTA CACGCCACTG GGCGTCTCTC
    AGTAACCTGG TGGCATCTCT GCTGAACTCC ATGAAGTCCA TTGCTTCTCT ACTGCTGCTG
    CTCTTCCTCT TCATCATCAT CTTTAGCCTC CTCGGTATGC AGGTGTTCGG CGGCAAGTTT
    AACTTTGACG AGACACAAAC CAAGAGGAGC ACCTTTGACA ACTTCCCTCA GGCCTTGCTC
    ACTGTGTTTC AGATTCTAAC TGGTGAAGAC TGGAATGCCG TCATGTATGA CGGAATTATG
    GCGTACGGCG GCCCTTCGTC ATCAGGAATG ATTGTGTGCA TATACTTCAT CATCCTTTTC
    ATCTGTGGAA ACTACATTCT CCTGAATGTG TTCTTGGCTA TCGCTGTGGA CAATCTTGCT
    GATGCAGAGT CTCTGAACAC GGACGATACG AAGAAACCTG ATGAAATCGA CGAAATTGAA
    GATGAAGCCA AGGCTGGAGA GGAGGATGAG AAGGATAATG CCGAGGAGGA TGAAGAAGAG
    CCAGATGTTC CTGCAGGGCC TCGTCCAAAG ATCTCAGAGC TTGTTAAGAA GGAGAAGATC
    ACTCCAATCC CAGAGGGCAG TGCGTTCTTC ATCTTCAGCA ACACAAACCC AGTTCGAGTC
    GCCTGCCACA AGCTGATAAA CCACCACATC TTCACCAACC TCATCCTTGT GTTCATTATG
    CTAAGCTCAG CGTCATTGGC TGCTGAGGAT CCAATCAGAA ACTTCTCTGC TAGGAATATT
    ATACTTGGTT ACTTTGACTA TGCTTTCACG GCTATCTTTA CTGTAGAGAT CGTGTTAAAG
    ATGACCACGT ATGGCGCTTT TCTCCATAAG GGTGCTTTCT GCAGGAACTA CTTTAATCTT
    CTAGATCTGC TGGTGGTCGG AGTTTCGCTC GTGTCCTTCG GGATTCAATC ATCGGCCATC
    TCAGTGGTGA AGATTCTGAG GGTTCTGAGA GTTTTGCGTC CACTCAGAGC CATAAACAGA
    GCTAAAGGAC TAAAGCATGT GGTCCAGTGT GTGTTTGTGG CCATCAGGAC TATAGGAAAC
    ATCATGATCG TCACCACTCT GCTGCAGTTT ATGTTCGCCT GCATTGGTGT CCAGCTCTTC
    AAGGGAAAGT TTTACCGTTG TAACGATGAG GCCAAGTCCA GCCCAGAGGA GTGCAAGGGA
    ACGTACATCA TGTATAAGGA AGGAGACGTG AACCAGCCAA TCATTCAGAA GCGCCACTGG
    CACAACAGTG ATTTCAACTT TGACAATGTG CTCATGGCCA TGATGGCTCT GTTCACCGTG
    TCCACGTTTG AAGGCTGGCC AGCGTTGCTG TACAAGGCCA TCGACTCCAA CCGGGAGAAC
    ATGGGTCCCA TCTACAACTA CCGAGTGGAG ATTTCCATCT TCTTCATCAT CTACATCATC
    ATCATCGCTT TCTTTATGAT GAACATCTTT GTGGGTTTCG TCATCGTGAC CTTTCAGGAA
    CAGGGAGAGA AGGAGTACAA GAACTGTGAG CTGGACAAGA ACCAGCGTCA GTGTGTGGAG
    TATGCATTGA AAGCACGTCC TCTCAGGAGG TACATCCCAA AGAACCCATA TCAGTATAAG
    TTCTGGTATG TGGTGAACTC CACCGGCTTC GAGTACATCA TGTTTGTCCT GATTTTACTC
    AACACCATCT GTCTCGCCGT GCAGCACTAT GGTCAGTCTG AGCTCTTCAA TTATGTTATG
    GACATTCTGA ATATGGTCTT CACTGCCGTC TTCACTGTGG AGATGGTGCT CAAGCTGATC
    GCCTTCAAGC CTCGGCATTA TTTCACTGAC GCATGGAACA CATTTGACGC CTTAATTGTT
    GTCGGTAGCG TCGTAGATAT TGCTATCACT GAAGTCAACC CCACAGAGGC ACCTCAGGTG
    GATGAGTCGG GGAACACTGA GGACAGCGCT CGCATTTCCA TCACTTTCTT CCGTCTTTTT
    CGAGTGATGC GATTGGTCAA GCTGCTGAGC AGAGGGGAGG GAATTCGAAC CCTACTCTGG
    ACCTTTATCA AATCCTTCCA GGCACTGCCT TATGTTGCTT TGCTGATAGC CATGCTCTTC
    TTCATCTACG CTGTCATTGG CATGCAGGTG TTTGGGAAAA TTGCCATGGT GGATCACACT
    CAAATCAACA GGAACAACAA CTTTCAGACG TTCCCTCAGG CTGTGCTTCT GCTCTTCAGG
    TGTGCTACGG GTGAAGCCTG GCAGGAGATC ATGTTAGCAT GTATGCCAGG CAAGCTGTGT
    GACCCGGAGT CAGACTATAA TCCTGGGGAA GAAATGACCT GCGGGAGCAG TTTCGCCATC
    ATCTACTTCA TCACCTTCTA CATGCTCTGC GCCTTCCTGA TCATTAACCT GTTTGTGGCT
    GTCATCATGG ACAACTTCGA CTATCTGACC CGTGATTGGT CAATTCTGGG GCCACATCAC
    CTTGATGAGT TTAAGAGGAT TTGGTCAGAA TATGACCCAG AGGCCAAGGG CAGAATAAAA
    CATCTTGATG TAGTGACGTT GTTGCGCCGT ATTCAGCCTC CTCTCGGCTT TGGGAAACTC
    TGTCCTCATA GAGTCGCCTG CAAGAGGCTG GTGGCTATGA ACATGCCCCT GAACAGTGAC
    GGCACGGTCA TGTTTAACGC GACACTGTTT GCTCTCGTGA GAACCGCATT GAAAATCAAA
    ACAGAAGGTA ACCTAGAACA AGCGAATGAA GAGCTCCGAG CTGTGATTAA GAAGATCTGG
    AAGAGAACCA GCATGAAGCT TCTGGATCAA GTGGTTCCCC CTGCTGGTGA TGATGAGGTA
    ACGGTGGGGA AGTTCTATGC CACTTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA
    CGCAAAGAGG AAGGCCTGGT GGGAGTTCAT CCGGCACAGA ACAACACGGC CATCGCACTG
    CAGGCTGGCC TTCGTACGCT GCATGACATT GGGCCAGAAA TCCGCCGTGC GATATCATGT
    GACCTGCAGG ATGATGAGCT AGTAGACTTT ATACCAGAGG AGGACGAAGA AATTTATAGG
    CGAAACGGAG GACTCTTTGG CAACCACATC AACCACATAA ACGGAGACCC CCGGAGGAGC
    TCTGGCCACC AAACAAACGC CACACAGCGC CCCCTGCAGG TGCAGCCGCC CCCACATTAC
    GTCCACATGG AGCAGCCCGT GGGGCGTCTG GGTCGCGCCA ATGCCATGGC ACAGCAAAAC
    CATCATCGTC ACCACCACCA CCATCACCAT CATCATCATC ACAACAACTC CTACAACAAA
    TCGCCCAAGT CCACCAACAT CAACCTCAAC AACGCCAACG TGTCCAGTTT ACCCAATGGT
    GGACACAATC GCTACTACGA ACACGCTCCC GCTAATGGCT ACCCAGGGTC ATATTACGGA
    GAATACGACA AGCCCAGGAC CCCTCATGGG CAAAGAAGGC GCTACTATGA GACATATATT
    AGATCGCAGG GCAGCGACAG AAGACGACCC ACGATACGCC GCGAAGAGGA GTATGAAGAA
    GATCGCTACT CTGGAGAGTA TTACAGCGGA GAGGAGTTTT ATGAAGATGA CAGCATGCTA
    TCGGGTGATA GGTACCCCAA CAGTGACCAG GAGTACGAGA CGCCGCGGGG TTACCATCAT
    CCTGACAGTT ACTATGAAGA TGATGAGCAG CCCCTTTATC ACGATTCACA TAGGTCACCA
    AAGAGAAGAC TGCTTCCTCC AACACCGCAA GGTAATCGGA GACCGTCGTT CAATTTTGAG
    TGTCTGCGCA GACAGAGCAG TCAAGACGAT CTTCCTCACC AGCGAACTGC TCTTCCGTTA
    CATCTCATGC AACACCAGGT GATGGCAGTG GCGGGTCTGG ACTCTAGCAG GGCTCACAGG
    CTTTCACCAA CTCGATCTAC CAGATCATGG GCTTCACCTC CTCCAACTCC TGCAAGTAAA
    GACCGGACGC CGTACTACAC ACCGCTCATC CGCGTGGACC GTCCTCTAAG AGACAGCGCC
    AGCAGCAGCC ATTCATCCAT CAGGAAGAGC TCGTGGTACA CAGACGACCC TGAGTACCAG
    CAGAGGAACT TCTCACCTGT TCACCTGCAG GTCCCACCCG AGTACAGGAA CCAGTACCTG
    CAGAAGAGAG GCAGCGCTAC CAGCCTGGTG GAGGCGGTCT TGATATCAGA AGGACTGGGT
    CGATACGCCA AGGATCCCAA ATTTGTGGCC GCCACAAAGC ATGAGATTGC GGACGCGTGC
    GAGATGACCA TTGACGAAAT GGAAAGCGCC GCCAGCCATC TTCTTAACGG TGGCATTACA
    CCAGTAGTCA ACGGAGTCAA TGTGTTTCCC ATACTGGGCC ACAGAGACTA CGAGCTGCAG
    GACGTCTCGG CGAGCTACAG CGACGAGGAG CCCGAACCCG AGCCCAGACC TCGTTACGAG
    GAGGACCTTG CAGATGAAAT GATATGCATT ACCACTTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147857
    Clone ID Related Accession (Same CDS sequence) XM_021479577.1
    Accession Version XM_021479577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCGGG TTACACTTGC CAGCCTCATG GAGAAGAAGA AGAAGGGGTT TGGCTGGTTC
    AGCCAGTCCT CTGACACTCA TGCGAGTATG CCGGCAAGTG AGACTGAATC CATGAATACA
    GAGAACGAGA AAGGAGAAGA TGAGAAGGCG ACATGTTGCG GCCCGACATG TCAAAAAATA
    TCAAAATCAA AGTTCAGTCG GCGCTGGCGT CGCTGGAACC GGTTGTGTCG GAGGAATTGT
    CGCTTGGCTG TAAAATCTGT GCCATTCTAT TGGCTGGTAA TCATCCTGGT GTTCCTGAAC
    ACTTTGACCA TCTCTTCAGA ACATTACAAC CAGCCCATGT GGCTCACACA AGTACAGGAT
    GTGGCTAATA AAGTGCTGCT GGCCATGTTC ACGTGTGAGA TGTTGGTGAA GATGTACAGT
    CTGGGCCTGC AGGCTTATTT TGTGTCTCTT TTTAATCGCT TTGACTGCTT TGTGGTGTGT
    GGAGGCATCA CTGAAACCAT CCTTGTGGAG TTTGAGATTA TGTCACCCCT CGGCATCTCA
    GTGTTTCGAT GTGTTCGACT TCTCAGGATC TTTAAAGTTA CACGCCACTG GGCGTCTCTC
    AGTAACCTGG TGGCATCTCT GCTGAACTCC ATGAAGTCCA TTGCTTCTCT ACTGCTGCTG
    CTCTTCCTCT TCATCATCAT CTTTAGCCTC CTCGGTATGC AGGTGTTCGG CGGCAAGTTT
    AACTTTGACG AGACACAAAC CAAGAGGAGC ACCTTTGACA ACTTCCCTCA GGCCTTGCTC
    ACTGTGTTTC AGATTCTAAC TGGTGAAGAC TGGAATGCCG TCATGTATGA CGGAATTATG
    GCGTACGGCG GCCCTTCGTC ATCAGGAATG ATTGTGTGCA TATACTTCAT CATCCTTTTC
    ATCTGTGGAA ACTACATTCT CCTGAATGTG TTCTTGGCTA TCGCTGTGGA CAATCTTGCT
    GATGCAGAGT CTCTGAACAC GGACGATACG AAGAAACCTG ATGAAATCGA CGAAATTGAA
    GATGAAGCCA AGGCTGGAGA GGAGGATGAG AAGGATAATG CCGAGGAGGA TGAAGAAGAG
    CCAGATGTTC CTGCAGGGCC TCGTCCAAAG ATCTCAGAGC TTGTTAAGAA GGAGAAGATC
    ACTCCAATCC CAGAGGGCAG TGCGTTCTTC ATCTTCAGCA ACACAAACCC AGTTCGAGTC
    GCCTGCCACA AGCTGATAAA CCACCACATC TTCACCAACC TCATCCTTGT GTTCATTATG
    CTAAGCTCAG CGTCATTGGC TGCTGAGGAT CCAATCAGAA ACTTCTCTGC TAGGAATATT
    GTCCTAGGCT ATGCAGATTA TGTCTTCACT AGTATGTTTA CATTTGAGAT CGTGTTAAAG
    ATGACCACGT ATGGCGCTTT TCTCCATAAG GGTGCTTTCT GCAGGAACTA CTTTAATCTT
    CTAGATCTGC TGGTGGTCGG AGTTTCGCTC GTGTCCTTCG GGATTCAATC ATCGGCCATC
    TCAGTGGTGA AGATTCTGAG GGTTCTGAGA GTTTTGCGTC CACTCAGAGC CATAAACAGA
    GCTAAAGGAC TAAAGCATGT GGTCCAGTGT GTGTTTGTGG CCATCAGGAC TATAGGAAAC
    ATCATGATCG TCACCACTCT GCTGCAGTTT ATGTTCGCCT GCATTGGTGT CCAGCTCTTC
    AAGGGAAAGT TTTACCGTTG TAACGATGAG GCCAAGTCCA GCCCAGAGGA GTGCAAGGGA
    ACGTACATCA TGTATAAGGA AGGAGACGTG AACCAGCCAA TCATTCAGAA GCGCCACTGG
    CACAACAGTG ATTTCAACTT TGACAATGTG CTCATGGCCA TGATGGCTCT GTTCACCGTG
    TCCACGTTTG AAGGCTGGCC AGCGTTGCTG TACAAGGCCA TCGACTCCAA CCGGGAGAAC
    ATGGGTCCCA TCTACAACTA CCGAGTGGAG ATTTCCATCT TCTTCATCAT CTACATCATC
    ATCATCGCTT TCTTTATGAT GAACATCTTT GTGGGTTTCG TCATCGTGAC CTTTCAGGAA
    CAGGGAGAGA AGGAGTACAA GAACTGTGAG CTGGACAAGA ACCAGCGTCA GTGTGTGGAG
    TATGCATTGA AAGCACGTCC TCTCAGGAGG TACATCCCAA AGAACCCATA TCAGTATAAG
    TTCTGGTATG TGGTGAACTC CACCGGCTTC GAGTACATCA TGTTTGTCCT GATTTTACTC
    AACACCATCT GTCTCGCCGT GCAGCACTAT GGTCAGTCTG AGCTCTTCAA TTATGTTATG
    GACATTCTGA ATATGGTCTT CACTGCCGTC TTCACTGTGG AGATGGTGCT CAAGCTGATC
    GCCTTCAAGC CTCGGCATTA TTTCACTGAC GCATGGAACA CATTTGACGC CTTAATTGTT
    GTCGGTAGCG TCGTAGATAT TGCTATCACT GAAGTCAACC CCACAGAGGC ACCTCAGGTG
    GATGAGTCGG GGAACACTGA GGACAGCGCT CGCATTTCCA TCACTTTCTT CCGTCTTTTT
    CGAGTGATGC GATTGGTCAA GCTGCTGAGC AGAGGGGAGG GAATTCGAAC CCTACTCTGG
    ACCTTTATCA AATCCTTCCA GGCACTGCCT TATGTTGCTT TGCTGATAGC CATGCTCTTC
    TTCATCTACG CTGTCATTGG CATGCAGGTG TTTGGGAAAA TTGCCATGGT GGATCACACT
    CAAATCAACA GGAACAACAA CTTTCAGACG TTCCCTCAGG CTGTGCTTCT GCTCTTCAGG
    TGTGCTACGG GTGAAGCCTG GCAGGAGATC ATGTTAGCAT GTATGCCAGG CAAGCTGTGT
    GACCCGGAGT CAGACTATAA TCCTGGGGAA GAAATGACCT GCGGGAGCAG TTTCGCCATC
    ATCTACTTCA TCACCTTCTA CATGCTCTGC GCCTTCCTGA TCATTAACCT GTTTGTGGCT
    GTCATCATGG ACAACTTCGA CTATCTGACC CGTGATTGGT CAATTCTGGG GCCACATCAC
    CTTGATGAGT TTAAGAGGAT TTGGTCAGAA TATGACCCAG AGGCCAAGGG CAGAATAAAA
    CATCTTGATG TAGTGACGTT GTTGCGCCGT ATTCAGCCTC CTCTCGGCTT TGGGAAACTC
    TGTCCTCATA GAGTCGCCTG CAAGAGGCTG GTGGCTATGA ACATGCCCCT GAACAGTGAC
    GGCACGGTCA TGTTTAACGC GACACTGTTT GCTCTCGTGA GAACCGCATT GAAAATCAAA
    ACAGAAGGTA ACCTAGAACA AGCGAATGAA GAGCTCCGAG CTGTGATTAA GAAGATCTGG
    AAGAGAACCA GCATGAAGCT TCTGGATCAA GTGGTTCCCC CTGCTGGTGA TGATGAGGTA
    ACGGTGGGGA AGTTCTATGC CACTTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA
    CGCAAAGAGG AAGGCCTGGT GGGAGTTCAT CCGGCACAGA ACAACACGGC CATCGCACTG
    CAGGCTGGCC TTCGTACGCT GCATGACATT GGGCCAGAAA TCCGCCGTGC GATATCATGT
    GACCTGCAGG ATGATGAGCT AGTAGACTTT ATACCAGAGG AGGACGAAGA AATTTATAGG
    CGAAACGGAG GACTCTTTGG CAACCACATC AACCACATAA ACGGAGACCC CCGGAGGAGC
    TCTGGCCACC AAACAAACGC CACACAGCGC CCCCTGCAGG TGCAGCCGCC CCCACATTAC
    GTCCACATGG AGCAGCCCGT GGGGCGTCTG GGTCGCGCCA ATGCCATGGC ACAGCAAAAC
    CATCATCGTC ACCACCACCA CCATCACCAT CATCATCATC ACAACAACTC CTACAACAAA
    TCGCCCAAGT CCACCAACAT CAACCTCAAC AACGCCAACG TGTCCAGTTT ACCCAATGGT
    GGACACAATC GCTACTACGA ACACGCTCCC GCTAATGGCT ACCCAGGGTC ATATTACGGA
    GAATACGACA AGCCCAGGAC CCCTCATGGG CAAAGAAGGC GCTACTATGA GACATATATT
    AGATCGCAGG GCAGCGACAG AAGACGACCC ACGATACGCC GCGAAGAGGA GTATGAAGAA
    GATCGCTACT CTGGAGAGTA TTACAGCGGA GAGGAGTTTT ATGAAGATGA CAGCATGCTA
    TCGGGTGATA GGTACCCCAA CAGTGACCAG GAGTACGAGA CGCCGCGGGG TTACCATCAT
    CCTGACAGTT ACTATGAAGA TGATGAGCAG CCCCTTTATC ACGATTCACA TAGGTCACCA
    AAGAGAAGAC TGCTTCCTCC AACACCGCAA GGTAATCGGA GACCGTCGTT CAATTTTGAG
    TGTCTGCGCA GACAGAGCAG TCAAGACGAT CTTCCTCACC AGCGAACTGC TCTTCCGTTA
    CATCTCATGC AACACCAGGT GATGGCAGTG GCGGGTCTGG ACTCTAGCAG GGCTCACAGG
    CTTTCACCAA CTCGATCTAC CAGATCATGG GCTTCACCTC CTCCAACTCC TGCAAGTAAA
    GACCGGACGC CGTACTACAC ACCGCTCATC CGCGTGGACC GTCCTCTAAG AGACAGCGCC
    AGCAGCAGCC ATTCATCCAT CAGGAAGAGC TCGTGGTACA CAGACGACCC TGAGTACCAG
    CAGAGGAACT TCTCACCTGT TCACCTGCAG GTCCCACCCG AGTACAGGAA CCAGTACCTG
    CAGAAGAGAG GCAGCGCTAC CAGCCTGGTG GAGGCGGTCT TGATATCAGA AGGACTGGGT
    CGATACGCCA AGGATCCCAA ATTTGTGGCC GCCACAAAGC ATGAGATTGC GGACGCGTGC
    GAGATGACCA TTGACGAAAT GGAAAGCGCC GCCAGCCATC TTCTTAACGG TGGCATTACA
    CCAGTAGTCA ACGGAGTCAA TGTGTTTCCC ATACTGGGCC ACAGAGACTA CGAGCTGCAG
    GACGTCTCGG CGAGCTACAG CGACGAGGAG CCCGAACCCG AGCCCAGACC TCGTTACGAG
    GAGGACCTTG CAGATGAAAT GATATGCATT ACCACTTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335252.1
    CDS411..6692
    Translation

    Target ORF information:

    RefSeq Version XM_021479577.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCGGG TTACACTTGC CAGCCTCATG GAGAAGAAGA AGAAGGGGTT TGGCTGGTTC
    AGCCAGTCCT CTGACACTCA TGCGAGTATG CCGGCAAGTG AGACTGAATC CATGAATACA
    GAGAACGAGA AAGGAGAAGA TGAGAAGGCG ACATGTTGCG GCCCGACATG TCAAAAAATA
    TCAAAATCAA AGTTCAGTCG GCGCTGGCGT CGCTGGAACC GGTTGTGTCG GAGGAATTGT
    CGCTTGGCTG TAAAATCTGT GCCATTCTAT TGGCTGGTAA TCATCCTGGT GTTCCTGAAC
    ACTTTGACCA TCTCTTCAGA ACATTACAAC CAGCCCATGT GGCTCACACA AGTACAGGAT
    GTGGCTAATA AAGTGCTGCT GGCCATGTTC ACGTGTGAGA TGTTGGTGAA GATGTACAGT
    CTGGGCCTGC AGGCTTATTT TGTGTCTCTT TTTAATCGCT TTGACTGCTT TGTGGTGTGT
    GGAGGCATCA CTGAAACCAT CCTTGTGGAG TTTGAGATTA TGTCACCCCT CGGCATCTCA
    GTGTTTCGAT GTGTTCGACT TCTCAGGATC TTTAAAGTTA CACGCCACTG GGCGTCTCTC
    AGTAACCTGG TGGCATCTCT GCTGAACTCC ATGAAGTCCA TTGCTTCTCT ACTGCTGCTG
    CTCTTCCTCT TCATCATCAT CTTTAGCCTC CTCGGTATGC AGGTGTTCGG CGGCAAGTTT
    AACTTTGACG AGACACAAAC CAAGAGGAGC ACCTTTGACA ACTTCCCTCA GGCCTTGCTC
    ACTGTGTTTC AGATTCTAAC TGGTGAAGAC TGGAATGCCG TCATGTATGA CGGAATTATG
    GCGTACGGCG GCCCTTCGTC ATCAGGAATG ATTGTGTGCA TATACTTCAT CATCCTTTTC
    ATCTGTGGAA ACTACATTCT CCTGAATGTG TTCTTGGCTA TCGCTGTGGA CAATCTTGCT
    GATGCAGAGT CTCTGAACAC GGACGATACG AAGAAACCTG ATGAAATCGA CGAAATTGAA
    GATGAAGCCA AGGCTGGAGA GGAGGATGAG AAGGATAATG CCGAGGAGGA TGAAGAAGAG
    CCAGATGTTC CTGCAGGGCC TCGTCCAAAG ATCTCAGAGC TTGTTAAGAA GGAGAAGATC
    ACTCCAATCC CAGAGGGCAG TGCGTTCTTC ATCTTCAGCA ACACAAACCC AGTTCGAGTC
    GCCTGCCACA AGCTGATAAA CCACCACATC TTCACCAACC TCATCCTTGT GTTCATTATG
    CTAAGCTCAG CGTCATTGGC TGCTGAGGAT CCAATCAGAA ACTTCTCTGC TAGGAATATT
    GTCCTAGGCT ATGCAGATTA TGTCTTCACT AGTATGTTTA CATTTGAGAT CGTGTTAAAG
    ATGACCACGT ATGGCGCTTT TCTCCATAAG GGTGCTTTCT GCAGGAACTA CTTTAATCTT
    CTAGATCTGC TGGTGGTCGG AGTTTCGCTC GTGTCCTTCG GGATTCAATC ATCGGCCATC
    TCAGTGGTGA AGATTCTGAG GGTTCTGAGA GTTTTGCGTC CACTCAGAGC CATAAACAGA
    GCTAAAGGAC TAAAGCATGT GGTCCAGTGT GTGTTTGTGG CCATCAGGAC TATAGGAAAC
    ATCATGATCG TCACCACTCT GCTGCAGTTT ATGTTCGCCT GCATTGGTGT CCAGCTCTTC
    AAGGGAAAGT TTTACCGTTG TAACGATGAG GCCAAGTCCA GCCCAGAGGA GTGCAAGGGA
    ACGTACATCA TGTATAAGGA AGGAGACGTG AACCAGCCAA TCATTCAGAA GCGCCACTGG
    CACAACAGTG ATTTCAACTT TGACAATGTG CTCATGGCCA TGATGGCTCT GTTCACCGTG
    TCCACGTTTG AAGGCTGGCC AGCGTTGCTG TACAAGGCCA TCGACTCCAA CCGGGAGAAC
    ATGGGTCCCA TCTACAACTA CCGAGTGGAG ATTTCCATCT TCTTCATCAT CTACATCATC
    ATCATCGCTT TCTTTATGAT GAACATCTTT GTGGGTTTCG TCATCGTGAC CTTTCAGGAA
    CAGGGAGAGA AGGAGTACAA GAACTGTGAG CTGGACAAGA ACCAGCGTCA GTGTGTGGAG
    TATGCATTGA AAGCACGTCC TCTCAGGAGG TACATCCCAA AGAACCCATA TCAGTATAAG
    TTCTGGTATG TGGTGAACTC CACCGGCTTC GAGTACATCA TGTTTGTCCT GATTTTACTC
    AACACCATCT GTCTCGCCGT GCAGCACTAT GGTCAGTCTG AGCTCTTCAA TTATGTTATG
    GACATTCTGA ATATGGTCTT CACTGCCGTC TTCACTGTGG AGATGGTGCT CAAGCTGATC
    GCCTTCAAGC CTCGGCATTA TTTCACTGAC GCATGGAACA CATTTGACGC CTTAATTGTT
    GTCGGTAGCG TCGTAGATAT TGCTATCACT GAAGTCAACC CCACAGAGGC ACCTCAGGTG
    GATGAGTCGG GGAACACTGA GGACAGCGCT CGCATTTCCA TCACTTTCTT CCGTCTTTTT
    CGAGTGATGC GATTGGTCAA GCTGCTGAGC AGAGGGGAGG GAATTCGAAC CCTACTCTGG
    ACCTTTATCA AATCCTTCCA GGCACTGCCT TATGTTGCTT TGCTGATAGC CATGCTCTTC
    TTCATCTACG CTGTCATTGG CATGCAGGTG TTTGGGAAAA TTGCCATGGT GGATCACACT
    CAAATCAACA GGAACAACAA CTTTCAGACG TTCCCTCAGG CTGTGCTTCT GCTCTTCAGG
    TGTGCTACGG GTGAAGCCTG GCAGGAGATC ATGTTAGCAT GTATGCCAGG CAAGCTGTGT
    GACCCGGAGT CAGACTATAA TCCTGGGGAA GAAATGACCT GCGGGAGCAG TTTCGCCATC
    ATCTACTTCA TCACCTTCTA CATGCTCTGC GCCTTCCTGA TCATTAACCT GTTTGTGGCT
    GTCATCATGG ACAACTTCGA CTATCTGACC CGTGATTGGT CAATTCTGGG GCCACATCAC
    CTTGATGAGT TTAAGAGGAT TTGGTCAGAA TATGACCCAG AGGCCAAGGG CAGAATAAAA
    CATCTTGATG TAGTGACGTT GTTGCGCCGT ATTCAGCCTC CTCTCGGCTT TGGGAAACTC
    TGTCCTCATA GAGTCGCCTG CAAGAGGCTG GTGGCTATGA ACATGCCCCT GAACAGTGAC
    GGCACGGTCA TGTTTAACGC GACACTGTTT GCTCTCGTGA GAACCGCATT GAAAATCAAA
    ACAGAAGGTA ACCTAGAACA AGCGAATGAA GAGCTCCGAG CTGTGATTAA GAAGATCTGG
    AAGAGAACCA GCATGAAGCT TCTGGATCAA GTGGTTCCCC CTGCTGGTGA TGATGAGGTA
    ACGGTGGGGA AGTTCTATGC CACTTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA
    CGCAAAGAGG AAGGCCTGGT GGGAGTTCAT CCGGCACAGA ACAACACGGC CATCGCACTG
    CAGGCTGGCC TTCGTACGCT GCATGACATT GGGCCAGAAA TCCGCCGTGC GATATCATGT
    GACCTGCAGG ATGATGAGCT AGTAGACTTT ATACCAGAGG AGGACGAAGA AATTTATAGG
    CGAAACGGAG GACTCTTTGG CAACCACATC AACCACATAA ACGGAGACCC CCGGAGGAGC
    TCTGGCCACC AAACAAACGC CACACAGCGC CCCCTGCAGG TGCAGCCGCC CCCACATTAC
    GTCCACATGG AGCAGCCCGT GGGGCGTCTG GGTCGCGCCA ATGCCATGGC ACAGCAAAAC
    CATCATCGTC ACCACCACCA CCATCACCAT CATCATCATC ACAACAACTC CTACAACAAA
    TCGCCCAAGT CCACCAACAT CAACCTCAAC AACGCCAACG TGTCCAGTTT ACCCAATGGT
    GGACACAATC GCTACTACGA ACACGCTCCC GCTAATGGCT ACCCAGGGTC ATATTACGGA
    GAATACGACA AGCCCAGGAC CCCTCATGGG CAAAGAAGGC GCTACTATGA GACATATATT
    AGATCGCAGG GCAGCGACAG AAGACGACCC ACGATACGCC GCGAAGAGGA GTATGAAGAA
    GATCGCTACT CTGGAGAGTA TTACAGCGGA GAGGAGTTTT ATGAAGATGA CAGCATGCTA
    TCGGGTGATA GGTACCCCAA CAGTGACCAG GAGTACGAGA CGCCGCGGGG TTACCATCAT
    CCTGACAGTT ACTATGAAGA TGATGAGCAG CCCCTTTATC ACGATTCACA TAGGTCACCA
    AAGAGAAGAC TGCTTCCTCC AACACCGCAA GGTAATCGGA GACCGTCGTT CAATTTTGAG
    TGTCTGCGCA GACAGAGCAG TCAAGACGAT CTTCCTCACC AGCGAACTGC TCTTCCGTTA
    CATCTCATGC AACACCAGGT GATGGCAGTG GCGGGTCTGG ACTCTAGCAG GGCTCACAGG
    CTTTCACCAA CTCGATCTAC CAGATCATGG GCTTCACCTC CTCCAACTCC TGCAAGTAAA
    GACCGGACGC CGTACTACAC ACCGCTCATC CGCGTGGACC GTCCTCTAAG AGACAGCGCC
    AGCAGCAGCC ATTCATCCAT CAGGAAGAGC TCGTGGTACA CAGACGACCC TGAGTACCAG
    CAGAGGAACT TCTCACCTGT TCACCTGCAG GTCCCACCCG AGTACAGGAA CCAGTACCTG
    CAGAAGAGAG GCAGCGCTAC CAGCCTGGTG GAGGCGGTCT TGATATCAGA AGGACTGGGT
    CGATACGCCA AGGATCCCAA ATTTGTGGCC GCCACAAAGC ATGAGATTGC GGACGCGTGC
    GAGATGACCA TTGACGAAAT GGAAAGCGCC GCCAGCCATC TTCTTAACGG TGGCATTACA
    CCAGTAGTCA ACGGAGTCAA TGTGTTTCCC ATACTGGGCC ACAGAGACTA CGAGCTGCAG
    GACGTCTCGG CGAGCTACAG CGACGAGGAG CCCGAACCCG AGCCCAGACC TCGTTACGAG
    GAGGACCTTG CAGATGAAAT GATATGCATT ACCACTTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147858
    Clone ID Related Accession (Same CDS sequence) XM_021479578.1
    Accession Version XM_021479578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCGGG TTACACTTGC CAGCCTCATG GAGAAGAAGA AGAAGGGGTT TGGCTGGTTC
    AGCCAGTCCT CTGACACTCA TGCGAGTATG CCGGCAAGTG AGACTGAATC CATGAATACA
    GAGAACGAGA AAGGAGAAGA TGAGAAGGCG ACATGTTGCG GCCCGACATG TCAAAAAATA
    TCAAAATCAA AGTTCAGTCG GCGCTGGCGT CGCTGGAACC GGTTGTGTCG GAGGAATTGT
    CGCTTGGCTG TAAAATCTGT GCCATTCTAT TGGCTGGTAA TCATCCTGGT GTTCCTGAAC
    ACTTTGACCA TCTCTTCAGA ACATTACAAC CAGCCCATGT GGCTCACACA AGTACAGGAT
    GTGGCTAATA AAGTGCTGCT GGCCATGTTC ACGTGTGAGA TGTTGGTGAA GATGTACAGT
    CTGGGCCTGC AGGCTTATTT TGTGTCTCTT TTTAATCGCT TTGACTGCTT TGTGGTGTGT
    GGAGGCATCA CTGAAACCAT CCTTGTGGAG TTTGAGATTA TGTCACCCCT CGGCATCTCA
    GTGTTTCGAT GTGTTCGACT TCTCAGGATC TTTAAAGTTA CACGCCACTG GGCGTCTCTC
    AGTAACCTGG TGGCATCTCT GCTGAACTCC ATGAAGTCCA TTGCTTCTCT ACTGCTGCTG
    CTCTTCCTCT TCATCATCAT CTTTAGCCTC CTCGGTATGC AGGTGTTCGG CGGCAAGTTT
    AACTTTGACG AGACACAAAC CAAGAGGAGC ACCTTTGACA ACTTCCCTCA GGCCTTGCTC
    ACTGTGTTTC AGATTCTAAC TGGTGAAGAC TGGAATGCCG TCATGTATGA CGGAATTATG
    GCGTACGGCG GCCCTTCGTC ATCAGGAATG ATTGTGTGCA TATACTTCAT CATCCTTTTC
    ATCTGTGGAA ACTACATTCT CCTGAATGTG TTCTTGGCTA TCGCTGTGGA CAATCTTGCT
    GATGCAGAGT CTCTGAACAC GGACGATACG AAGAAACCTG ATGAAATCGA CGAAATTGAA
    GATGAAGCCA AGGCTGGAGA GGAGGATGAG AAGGATAATG CCGAGGAGGA TGAAGAAGAG
    CCAGATGTTC CTGCAGGGCC TCGTCCAAAG ATCTCAGAGC TTGTTAAGAA GGAGAAGATC
    ACTCCAATCC CAGAGGGCAG TGCGTTCTTC ATCTTCAGCA ACACAAACCC AGTTCGAGTC
    GCCTGCCACA AGCTGATAAA CCACCACATC TTCACCAACC TCATCCTTGT GTTCATTATG
    CTAAGCTCAG CGTCATTGGC TGCTGAGGAT CCAATCAGAA ACTTCTCTGC TAGGAATATT
    ATACTTGGTT ACTTTGACTA TGCTTTCACG GCTATCTTTA CTGTAGAGAT CGTGTTAAAG
    ATGACCACGT ATGGCGCTTT TCTCCATAAG GGTGCTTTCT GCAGGAACTA CTTTAATCTT
    CTAGATCTGC TGGTGGTCGG AGTTTCGCTC GTGTCCTTCG GGATTCAATC ATCGGCCATC
    TCAGTGGTGA AGATTCTGAG GGTTCTGAGA GTTTTGCGTC CACTCAGAGC CATAAACAGA
    GCTAAAGGAC TAAAGCATGT GGTCCAGTGT GTGTTTGTGG CCATCAGGAC TATAGGAAAC
    ATCATGATCG TCACCACTCT GCTGCAGTTT ATGTTCGCCT GCATTGGTGT CCAGCTCTTC
    AAGGGAAAGT TTTACCGTTG TAACGATGAG GCCAAGTCCA GCCCAGAGGA GTGCAAGGGA
    ACGTACATCA TGTATAAGGA AGGAGACGTG AACCAGCCAA TCATTCAGAA GCGCCACTGG
    CACAACAGTG ATTTCAACTT TGACAATGTG CTCATGGCCA TGATGGCTCT GTTCACCGTG
    TCCACGTTTG AAGGCTGGCC AGCGTTGCTG TACAAGGCCA TCGACTCCAA CCGGGAGAAC
    ATGGGTCCCA TCTACAACTA CCGAGTGGAG ATTTCCATCT TCTTCATCAT CTACATCATC
    ATCATCGCTT TCTTTATGAT GAACATCTTT GTGGGTTTCG TCATCGTGAC CTTTCAGGAA
    CAGGGAGAGA AGGAGTACAA GAACTGTGAG CTGGACAAGA ACCAGCGTCA GTGTGTGGAG
    TATGCATTGA AAGCACGTCC TCTCAGGAGG TACATCCCAA AGAACCCATA TCAGTATAAG
    TTCTGGTATG TGGTGAACTC CACCGGCTTC GAGTACATCA TGTTTGTCCT GATTTTACTC
    AACACCATCT GTCTCGCCGT GCAGCACTAT GGTCAGTCTG AGCTCTTCAA TTATGTTATG
    GACATTCTGA ATATGGTCTT CACTGCCGTC TTCACTGTGG AGATGGTGCT CAAGCTGATC
    GCCTTCAAGC CTCGGCATTA TTTCACTGAC GCATGGAACA CATTTGACGC CTTAATTGTT
    GTCGGTAGCG TCGTAGATAT TGCTATCACT GAAGTCAACC CCACAGAGGC ACCTCAGGTG
    GATGAGTCGG GGAACACTGA GGACAGCGCT CGCATTTCCA TCACTTTCTT CCGTCTTTTT
    CGAGTGATGC GATTGGTCAA GCTGCTGAGC AGAGGGGAGG GAATTCGAAC CCTACTCTGG
    ACCTTTATCA AATCCTTCCA GGCACTGCCT TATGTTGCTT TGCTGATAGC CATGCTCTTC
    TTCATCTACG CTGTCATTGG CATGCAGGTG TTTGGGAAAA TTGCCATGGT GGATCACACT
    CAAATCAACA GGAACAACAA CTTTCAGACG TTCCCTCAGG CTGTGCTTCT GCTCTTCAGG
    TGTGCTACGG GTGAAGCCTG GCAGGAGATC ATGTTAGCAT GTATGCCAGG CAAGCTGTGT
    GACCCGGAGT CAGACTATAA TCCTGGGGAA GAAATGACCT GCGGGAGCAG TTTCGCCATC
    ATCTACTTCA TCACCTTCTA CATGCTCTGC GCCTTCCTGA TCATTAACCT GTTTGTGGCT
    GTCATCATGG ACAACTTCGA CTATCTGACC CGTGATTGGT CAATTCTGGG GCCACATCAC
    CTTGATGAGT TTAAGAGGAT TTGGTCAGAA TATGACCCAG AGGCCAAGGG CAGAATAAAA
    CATCTTGATG TAGTGACGTT GTTGCGCCGT ATTCAGCCTC CTCTCGGCTT TGGGAAACTC
    TGTCCTCATA GAGTCGCCTG CAAGAGGCTG GTGGCTATGA ACATGCCCCT GAACAGTGAC
    GGCACGGTCA TGTTTAACGC GACACTGTTT GCTCTCGTGA GAACCGCATT GAAAATCAAA
    ACAGAAGGTA ACCTAGAACA AGCGAATGAA GAGCTCCGAG CTGTGATTAA GAAGATCTGG
    AAGAGAACCA GCATGAAGCT TCTGGATCAA GTGGTTCCCC CTGCTGGTGA TGATGAGGTA
    ACGGTGGGGA AGTTCTATGC CACTTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA
    CGCAAAGAGG AAGGCCTGGT GGGAGTTCAT CCGGCACAGA ACAACACGGC CATCGCACTG
    CAGGCTGGCC TTCGTACGCT GCATGACATT GGGCCAGAAA TCCGCCGTGC GATATCATGT
    GACCTGCAGG ATGATGAGCT AGTAGACTTT ATACCAGAGG AGGACGAAGA AATTTATAGG
    CGAAACGGAG GACTCTTTGG CAACCACATC AACCACATAA ACGGAGACCC CCGGAGGAGC
    TCTGGCCACC AAACAAACGC CACACAGCGC CCCCTGCAGG TGCAGCCGCC CCCACATTAC
    GTCCACATGG AGCAGCCCGT GGGGCGTCTG GGTCGCGCCA ATGCCATGGC ACAGCAAAAC
    CATCATCGTC ACCACCACCA CCATCACCAT CATCATCATC ACAACAACTC CTACAACAAA
    TCGCCCAAGT CCACCAACAT CAACCTCAAC AACGCCAACG TGTCCAGTTT ACCCAATGGT
    GGACACAATC GCTACTACGA ACACGCTCCC GCTAATGGCT ACCCAGGGTC ATATTACGGA
    GAATACGACA AGCCCAGGAC CCCTCATGGG CAAAGGCGCT ACTATGAGAC ATATATTAGA
    TCGCAGGGCA GCGACAGAAG ACGACCCACG ATACGCCGCG AAGAGGAGTA TGAAGAAGAT
    CGCTACTCTG GAGAGTATTA CAGCGGAGAG GAGTTTTATG AAGATGACAG CATGCTATCG
    GGTGATAGGT ACCCCAACAG TGACCAGGAG TACGAGACGC CGCGGGGTTA CCATCATCCT
    GACAGTTACT ATGAAGATGA TGAGCAGCCC CTTTATCACG ATTCACATAG GTCACCAAAG
    AGAAGACTGC TTCCTCCAAC ACCGCAAGGT AATCGGAGAC CGTCGTTCAA TTTTGAGTGT
    CTGCGCAGAC AGAGCAGTCA AGACGATCTT CCTCACCAGC GAACTGCTCT TCCGTTACAT
    CTCATGCAAC ACCAGGTGAT GGCAGTGGCG GGTCTGGACT CTAGCAGGGC TCACAGGCTT
    TCACCAACTC GATCTACCAG ATCATGGGCT TCACCTCCTC CAACTCCTGC AAGTAAAGAC
    CGGACGCCGT ACTACACACC GCTCATCCGC GTGGACCGTC CTCTAAGAGA CAGCGCCAGC
    AGCAGCCATT CATCCATCAG GAAGAGCTCG TGGTACACAG ACGACCCTGA GTACCAGCAG
    AGGAACTTCT CACCTGTTCA CCTGCAGGTC CCACCCGAGT ACAGGAACCA GTACCTGCAG
    AAGAGAGGCA GCGCTACCAG CCTGGTGGAG GCGGTCTTGA TATCAGAAGG ACTGGGTCGA
    TACGCCAAGG ATCCCAAATT TGTGGCCGCC ACAAAGCATG AGATTGCGGA CGCGTGCGAG
    ATGACCATTG ACGAAATGGA AAGCGCCGCC AGCCATCTTC TTAACGGTGG CATTACACCA
    GTAGTCAACG GAGTCAATGT GTTTCCCATA CTGGGCCACA GAGACTACGA GCTGCAGGAC
    GTCTCGGCGA GCTACAGCGA CGAGGAGCCC GAACCCGAGC CCAGACCTCG TTACGAGGAG
    GACCTTGCAG ATGAAATGAT ATGCATTACC ACTTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335253.1
    CDS411..6689
    Translation

    Target ORF information:

    RefSeq Version XM_021479578.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCGGG TTACACTTGC CAGCCTCATG GAGAAGAAGA AGAAGGGGTT TGGCTGGTTC
    AGCCAGTCCT CTGACACTCA TGCGAGTATG CCGGCAAGTG AGACTGAATC CATGAATACA
    GAGAACGAGA AAGGAGAAGA TGAGAAGGCG ACATGTTGCG GCCCGACATG TCAAAAAATA
    TCAAAATCAA AGTTCAGTCG GCGCTGGCGT CGCTGGAACC GGTTGTGTCG GAGGAATTGT
    CGCTTGGCTG TAAAATCTGT GCCATTCTAT TGGCTGGTAA TCATCCTGGT GTTCCTGAAC
    ACTTTGACCA TCTCTTCAGA ACATTACAAC CAGCCCATGT GGCTCACACA AGTACAGGAT
    GTGGCTAATA AAGTGCTGCT GGCCATGTTC ACGTGTGAGA TGTTGGTGAA GATGTACAGT
    CTGGGCCTGC AGGCTTATTT TGTGTCTCTT TTTAATCGCT TTGACTGCTT TGTGGTGTGT
    GGAGGCATCA CTGAAACCAT CCTTGTGGAG TTTGAGATTA TGTCACCCCT CGGCATCTCA
    GTGTTTCGAT GTGTTCGACT TCTCAGGATC TTTAAAGTTA CACGCCACTG GGCGTCTCTC
    AGTAACCTGG TGGCATCTCT GCTGAACTCC ATGAAGTCCA TTGCTTCTCT ACTGCTGCTG
    CTCTTCCTCT TCATCATCAT CTTTAGCCTC CTCGGTATGC AGGTGTTCGG CGGCAAGTTT
    AACTTTGACG AGACACAAAC CAAGAGGAGC ACCTTTGACA ACTTCCCTCA GGCCTTGCTC
    ACTGTGTTTC AGATTCTAAC TGGTGAAGAC TGGAATGCCG TCATGTATGA CGGAATTATG
    GCGTACGGCG GCCCTTCGTC ATCAGGAATG ATTGTGTGCA TATACTTCAT CATCCTTTTC
    ATCTGTGGAA ACTACATTCT CCTGAATGTG TTCTTGGCTA TCGCTGTGGA CAATCTTGCT
    GATGCAGAGT CTCTGAACAC GGACGATACG AAGAAACCTG ATGAAATCGA CGAAATTGAA
    GATGAAGCCA AGGCTGGAGA GGAGGATGAG AAGGATAATG CCGAGGAGGA TGAAGAAGAG
    CCAGATGTTC CTGCAGGGCC TCGTCCAAAG ATCTCAGAGC TTGTTAAGAA GGAGAAGATC
    ACTCCAATCC CAGAGGGCAG TGCGTTCTTC ATCTTCAGCA ACACAAACCC AGTTCGAGTC
    GCCTGCCACA AGCTGATAAA CCACCACATC TTCACCAACC TCATCCTTGT GTTCATTATG
    CTAAGCTCAG CGTCATTGGC TGCTGAGGAT CCAATCAGAA ACTTCTCTGC TAGGAATATT
    ATACTTGGTT ACTTTGACTA TGCTTTCACG GCTATCTTTA CTGTAGAGAT CGTGTTAAAG
    ATGACCACGT ATGGCGCTTT TCTCCATAAG GGTGCTTTCT GCAGGAACTA CTTTAATCTT
    CTAGATCTGC TGGTGGTCGG AGTTTCGCTC GTGTCCTTCG GGATTCAATC ATCGGCCATC
    TCAGTGGTGA AGATTCTGAG GGTTCTGAGA GTTTTGCGTC CACTCAGAGC CATAAACAGA
    GCTAAAGGAC TAAAGCATGT GGTCCAGTGT GTGTTTGTGG CCATCAGGAC TATAGGAAAC
    ATCATGATCG TCACCACTCT GCTGCAGTTT ATGTTCGCCT GCATTGGTGT CCAGCTCTTC
    AAGGGAAAGT TTTACCGTTG TAACGATGAG GCCAAGTCCA GCCCAGAGGA GTGCAAGGGA
    ACGTACATCA TGTATAAGGA AGGAGACGTG AACCAGCCAA TCATTCAGAA GCGCCACTGG
    CACAACAGTG ATTTCAACTT TGACAATGTG CTCATGGCCA TGATGGCTCT GTTCACCGTG
    TCCACGTTTG AAGGCTGGCC AGCGTTGCTG TACAAGGCCA TCGACTCCAA CCGGGAGAAC
    ATGGGTCCCA TCTACAACTA CCGAGTGGAG ATTTCCATCT TCTTCATCAT CTACATCATC
    ATCATCGCTT TCTTTATGAT GAACATCTTT GTGGGTTTCG TCATCGTGAC CTTTCAGGAA
    CAGGGAGAGA AGGAGTACAA GAACTGTGAG CTGGACAAGA ACCAGCGTCA GTGTGTGGAG
    TATGCATTGA AAGCACGTCC TCTCAGGAGG TACATCCCAA AGAACCCATA TCAGTATAAG
    TTCTGGTATG TGGTGAACTC CACCGGCTTC GAGTACATCA TGTTTGTCCT GATTTTACTC
    AACACCATCT GTCTCGCCGT GCAGCACTAT GGTCAGTCTG AGCTCTTCAA TTATGTTATG
    GACATTCTGA ATATGGTCTT CACTGCCGTC TTCACTGTGG AGATGGTGCT CAAGCTGATC
    GCCTTCAAGC CTCGGCATTA TTTCACTGAC GCATGGAACA CATTTGACGC CTTAATTGTT
    GTCGGTAGCG TCGTAGATAT TGCTATCACT GAAGTCAACC CCACAGAGGC ACCTCAGGTG
    GATGAGTCGG GGAACACTGA GGACAGCGCT CGCATTTCCA TCACTTTCTT CCGTCTTTTT
    CGAGTGATGC GATTGGTCAA GCTGCTGAGC AGAGGGGAGG GAATTCGAAC CCTACTCTGG
    ACCTTTATCA AATCCTTCCA GGCACTGCCT TATGTTGCTT TGCTGATAGC CATGCTCTTC
    TTCATCTACG CTGTCATTGG CATGCAGGTG TTTGGGAAAA TTGCCATGGT GGATCACACT
    CAAATCAACA GGAACAACAA CTTTCAGACG TTCCCTCAGG CTGTGCTTCT GCTCTTCAGG
    TGTGCTACGG GTGAAGCCTG GCAGGAGATC ATGTTAGCAT GTATGCCAGG CAAGCTGTGT
    GACCCGGAGT CAGACTATAA TCCTGGGGAA GAAATGACCT GCGGGAGCAG TTTCGCCATC
    ATCTACTTCA TCACCTTCTA CATGCTCTGC GCCTTCCTGA TCATTAACCT GTTTGTGGCT
    GTCATCATGG ACAACTTCGA CTATCTGACC CGTGATTGGT CAATTCTGGG GCCACATCAC
    CTTGATGAGT TTAAGAGGAT TTGGTCAGAA TATGACCCAG AGGCCAAGGG CAGAATAAAA
    CATCTTGATG TAGTGACGTT GTTGCGCCGT ATTCAGCCTC CTCTCGGCTT TGGGAAACTC
    TGTCCTCATA GAGTCGCCTG CAAGAGGCTG GTGGCTATGA ACATGCCCCT GAACAGTGAC
    GGCACGGTCA TGTTTAACGC GACACTGTTT GCTCTCGTGA GAACCGCATT GAAAATCAAA
    ACAGAAGGTA ACCTAGAACA AGCGAATGAA GAGCTCCGAG CTGTGATTAA GAAGATCTGG
    AAGAGAACCA GCATGAAGCT TCTGGATCAA GTGGTTCCCC CTGCTGGTGA TGATGAGGTA
    ACGGTGGGGA AGTTCTATGC CACTTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA
    CGCAAAGAGG AAGGCCTGGT GGGAGTTCAT CCGGCACAGA ACAACACGGC CATCGCACTG
    CAGGCTGGCC TTCGTACGCT GCATGACATT GGGCCAGAAA TCCGCCGTGC GATATCATGT
    GACCTGCAGG ATGATGAGCT AGTAGACTTT ATACCAGAGG AGGACGAAGA AATTTATAGG
    CGAAACGGAG GACTCTTTGG CAACCACATC AACCACATAA ACGGAGACCC CCGGAGGAGC
    TCTGGCCACC AAACAAACGC CACACAGCGC CCCCTGCAGG TGCAGCCGCC CCCACATTAC
    GTCCACATGG AGCAGCCCGT GGGGCGTCTG GGTCGCGCCA ATGCCATGGC ACAGCAAAAC
    CATCATCGTC ACCACCACCA CCATCACCAT CATCATCATC ACAACAACTC CTACAACAAA
    TCGCCCAAGT CCACCAACAT CAACCTCAAC AACGCCAACG TGTCCAGTTT ACCCAATGGT
    GGACACAATC GCTACTACGA ACACGCTCCC GCTAATGGCT ACCCAGGGTC ATATTACGGA
    GAATACGACA AGCCCAGGAC CCCTCATGGG CAAAGGCGCT ACTATGAGAC ATATATTAGA
    TCGCAGGGCA GCGACAGAAG ACGACCCACG ATACGCCGCG AAGAGGAGTA TGAAGAAGAT
    CGCTACTCTG GAGAGTATTA CAGCGGAGAG GAGTTTTATG AAGATGACAG CATGCTATCG
    GGTGATAGGT ACCCCAACAG TGACCAGGAG TACGAGACGC CGCGGGGTTA CCATCATCCT
    GACAGTTACT ATGAAGATGA TGAGCAGCCC CTTTATCACG ATTCACATAG GTCACCAAAG
    AGAAGACTGC TTCCTCCAAC ACCGCAAGGT AATCGGAGAC CGTCGTTCAA TTTTGAGTGT
    CTGCGCAGAC AGAGCAGTCA AGACGATCTT CCTCACCAGC GAACTGCTCT TCCGTTACAT
    CTCATGCAAC ACCAGGTGAT GGCAGTGGCG GGTCTGGACT CTAGCAGGGC TCACAGGCTT
    TCACCAACTC GATCTACCAG ATCATGGGCT TCACCTCCTC CAACTCCTGC AAGTAAAGAC
    CGGACGCCGT ACTACACACC GCTCATCCGC GTGGACCGTC CTCTAAGAGA CAGCGCCAGC
    AGCAGCCATT CATCCATCAG GAAGAGCTCG TGGTACACAG ACGACCCTGA GTACCAGCAG
    AGGAACTTCT CACCTGTTCA CCTGCAGGTC CCACCCGAGT ACAGGAACCA GTACCTGCAG
    AAGAGAGGCA GCGCTACCAG CCTGGTGGAG GCGGTCTTGA TATCAGAAGG ACTGGGTCGA
    TACGCCAAGG ATCCCAAATT TGTGGCCGCC ACAAAGCATG AGATTGCGGA CGCGTGCGAG
    ATGACCATTG ACGAAATGGA AAGCGCCGCC AGCCATCTTC TTAACGGTGG CATTACACCA
    GTAGTCAACG GAGTCAATGT GTTTCCCATA CTGGGCCACA GAGACTACGA GCTGCAGGAC
    GTCTCGGCGA GCTACAGCGA CGAGGAGCCC GAACCCGAGC CCAGACCTCG TTACGAGGAG
    GACCTTGCAG ATGAAATGAT ATGCATTACC ACTTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147859
    Clone ID Related Accession (Same CDS sequence) XM_021479579.1
    Accession Version XM_021479579.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCGGG TTACACTTGC CAGCCTCATG GAGAAGAAGA AGAAGGGGTT TGGCTGGTTC
    AGCCAGTCCT CTGACACTCA TGCGAGTATG CCGGCAAGTG AGACTGAATC CATGAATACA
    GAGAACGAGA AAGGAGAAGA TGAGAAGGCG ACATGTTGCG GCCCGACATG TCAAAAAATA
    TCAAAATCAA AGTTCAGTCG GCGCTGGCGT CGCTGGAACC GGTTGTGTCG GAGGAATTGT
    CGCTTGGCTG TAAAATCTGT GCCATTCTAT TGGCTGGTAA TCATCCTGGT GTTCCTGAAC
    ACTTTGACCA TCTCTTCAGA ACATTACAAC CAGCCCATGT GGCTCACACA AGTACAGGAT
    GTGGCTAATA AAGTGCTGCT GGCCATGTTC ACGTGTGAGA TGTTGGTGAA GATGTACAGT
    CTGGGCCTGC AGGCTTATTT TGTGTCTCTT TTTAATCGCT TTGACTGCTT TGTGGTGTGT
    GGAGGCATCA CTGAAACCAT CCTTGTGGAG TTTGAGATTA TGTCACCCCT CGGCATCTCA
    GTGTTTCGAT GTGTTCGACT TCTCAGGATC TTTAAAGTTA CACGCCACTG GGCGTCTCTC
    AGTAACCTGG TGGCATCTCT GCTGAACTCC ATGAAGTCCA TTGCTTCTCT ACTGCTGCTG
    CTCTTCCTCT TCATCATCAT CTTTAGCCTC CTCGGTATGC AGGTGTTCGG CGGCAAGTTT
    AACTTTGACG AGACACAAAC CAAGAGGAGC ACCTTTGACA ACTTCCCTCA GGCCTTGCTC
    ACTGTGTTTC AGATTCTAAC TGGTGAAGAC TGGAATGCCG TCATGTATGA CGGAATTATG
    GCGTACGGCG GCCCTTCGTC ATCAGGAATG ATTGTGTGCA TATACTTCAT CATCCTTTTC
    ATCTGTGGAA ACTACATTCT CCTGAATGTG TTCTTGGCTA TCGCTGTGGA CAATCTTGCT
    GATGCAGAGT CTCTGAACAC GGACGATACG AAGAAACCTG ATGAAATCGA CGAAATTGAA
    GATGAAGCCA AGGCTGGAGA GGAGGATGAG AAGGATAATG CCGAGGAGGA TGAAGAAGAG
    CCAGATGTTC CTGCAGGGCC TCGTCCAAAG ATCTCAGAGC TTGTTAAGAA GGAGAAGATC
    ACTCCAATCC CAGAGGGCAG TGCGTTCTTC ATCTTCAGCA ACACAAACCC AGTTCGAGTC
    GCCTGCCACA AGCTGATAAA CCACCACATC TTCACCAACC TCATCCTTGT GTTCATTATG
    CTAAGCTCAG CGTCATTGGC TGCTGAGGAT CCAATCAGAA ACTTCTCTGC TAGGAATATT
    ATACTTGGTT ACTTTGACTA TGCTTTCACG GCTATCTTTA CTGTAGAGAT CGTGTTAAAG
    ATGACCACGT ATGGCGCTTT TCTCCATAAG GGTGCTTTCT GCAGGAACTA CTTTAATCTT
    CTAGATCTGC TGGTGGTCGG AGTTTCGCTC GTGTCCTTCG GGATTCAATC ATCGGCCATC
    TCAGTGGTGA AGATTCTGAG GGTTCTGAGA GTTTTGCGTC CACTCAGAGC CATAAACAGA
    GCTAAAGGAC TAAAGCATGT GGTCCAGTGT GTGTTTGTGG CCATCAGGAC TATAGGAAAC
    ATCATGATCG TCACCACTCT GCTGCAGTTT ATGTTCGCCT GCATTGGTGT CCAGCTCTTC
    AAGGGAAAGT TTTACCGTTG TAACGATGAG GCCAAGTCCA GCCCAGAGGA GTGCAAGGGA
    ACGTACATCA TGTATAAGGA AGGAGACGTG AACCAGCCAA TCATTCAGAA GCGCCACTGG
    CACAACAGTG ATTTCAACTT TGACAATGTG CTCATGGCCA TGATGGCTCT GTTCACCGTG
    TCCACGTTTG AAGGCTGGCC AGCGTTGCTG TACAAGGCCA TCGACTCCAA CCGGGAGAAC
    ATGGGTCCCA TCTACAACTA CCGAGTGGAG ATTTCCATCT TCTTCATCAT CTACATCATC
    ATCATCGCTT TCTTTATGAT GAACATCTTT GTGGGTTTCG TCATCGTGAC CTTTCAGGAA
    CAGGGAGAGA AGGAGTACAA GAACTGTGAG CTGGACAAGA ACCAGCGTCA GTGTGTGGAG
    TATGCATTGA AAGCACGTCC TCTCAGGAGG TACATCCCAA AGAACCCATA TCAGTATAAG
    TTCTGGTATG TGGTGAACTC CACCGGCTTC GAGTACATCA TGTTTGTCCT GATTTTACTC
    AACACCATCT GTCTCGCCGT GCAGCACTAT GGTCAGTCTG AGCTCTTCAA TTATGTTATG
    GACATTCTGA ATATGGTCTT CACTGCCGTC TTCACTGTGG AGATGGTGCT CAAGCTGATC
    GCCTTCAAGC CTCGGCATTA TTTCACTGAC GCATGGAACA CATTTGACGC CTTAATTGTT
    GTCGGTAGCG TCGTAGATAT TGCTATCACT GAAGTCAACC CCACAGAGGC ACCTCAGGTG
    GATGAGTCGG GGAACACTGA GGACAGCGCT CGCATTTCCA TCACTTTCTT CCGTCTTTTT
    CGAGTGATGC GATTGGTCAA GCTGCTGAGC AGAGGGGAGG GAATTCGAAC CCTACTCTGG
    ACCTTTATCA AATCCTTCCA GGCACTGCCT TATGTTGCTT TGCTGATAGC CATGCTCTTC
    TTCATCTACG CTGTCATTGG CATGCAGGTG TTTGGGAAAA TTGCCATGGT GGATCACACT
    CAAATCAACA GGAACAACAA CTTTCAGACG TTCCCTCAGG CTGTGCTTCT GCTCTTCAGG
    TGTGCTACGG GTGAAGCCTG GCAGGAGATC ATGTTAGCAT GTATGCCAGG CAAGCTGTGT
    GACCCGGAGT CAGACTATAA TCCTGGGGAA GAAATGACCT GCGGGAGCAG TTTCGCCATC
    ATCTACTTCA TCACCTTCTA CATGCTCTGC GCCTTCCTGA TCATTAACCT GTTTGTGGCT
    GTCATCATGG ACAACTTCGA CTATCTGACC CGTGATTGGT CAATTCTGGG GCCACATCAC
    CTTGATGAGT TTAAGAGGAT TTGGTCAGAA TATGACCCAG AGGCCAAGGG CAGAATAAAA
    CATCTTGATG TAGTGACGTT GTTGCGCCGT ATTCAGCCTC CTCTCGGCTT TGGGAAACTC
    TGTCCTCATA GAGTCGCCTG CAAGAGGCTG GTGGCTATGA ACATGCCCCT GAACAGTGAC
    GGCACGGTCA TGTTTAACGC GACACTGTTT GCTCTCGTGA GAACCGCATT GAAAATCAAA
    ACAGAAGGTA ACCTAGAACA AGCGAATGAA GAGCTCCGAG CTGTGATTAA GAAGATCTGG
    AAGAGAACCA GCATGAAGCT TCTGGATCAA GTGGTTCCCC CTGCTGGTGA TGATGAGGTA
    ACGGTGGGGA AGTTCTATGC CACTTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA
    CGCAAAGAGG AAGGCCTGGT GGGAGTTCAT CCGGCACAGA ACAACACGGC CATCGCACTG
    CAGGCTGGCC TTCGTACGCT GCATGACATT GGGCCAGAAA TCCGCCGTGC GATATCATGT
    GACCTGCAGG ATGATGAGCT AGTAGACTTT ATACCAGAGG AGGACGAAGA AATTTATAGG
    CGAAACGGAG GACTCTTTGG CAACCACATC AACCACATAA ACGGAGACCC CCGGAGGAGC
    TCTGGCCACC AAACAAACGC CACACAGCGC CCCCTGCAGG TGCAGCCGCC CCCACATTAC
    GTCCACATGG AGCAGCCCGT GGGGCGTCTG GGTCGCGCCA ATGCCATGGC ACAGCAAAAC
    CATCATCGTC ACCACCACCA CCATCACCAT CATCATCATC ACAACAACTC CTACAACAAA
    TCGCCCAAGT CCACCAACAT CAACCTCAAC AACGCCAACG TGTCCAGTTT ACCCAATGGT
    GGACACAATC GCTACTACGA ACACGCTCCC GCTAATGGCT ACCCAGGGTC ATATTACGGA
    GAATACGACA AGCCCAGGAC CCCTCATGGG CAAAGATCGC AGGGCAGCGA CAGAAGACGA
    CCCACGATAC GCCGCGAAGA GGAGTATGAA GAAGATCGCT ACTCTGGAGA GTATTACAGC
    GGAGAGGAGT TTTATGAAGA TGACAGCATG CTATCGGGTG ATAGGTACCC CAACAGTGAC
    CAGGAGTACG AGACGCCGCG GGGTTACCAT CATCCTGACA GTTACTATGA AGATGATGAG
    CAGCCCCTTT ATCACGATTC ACATAGGTCA CCAAAGAGAA GACTGCTTCC TCCAACACCG
    CAAGGTAATC GGAGACCGTC GTTCAATTTT GAGTGTCTGC GCAGACAGAG CAGTCAAGAC
    GATCTTCCTC ACCAGCGAAC TGCTCTTCCG TTACATCTCA TGCAACACCA GGTGATGGCA
    GTGGCGGGTC TGGACTCTAG CAGGGCTCAC AGGCTTTCAC CAACTCGATC TACCAGATCA
    TGGGCTTCAC CTCCTCCAAC TCCTGCAAGT AAAGACCGGA CGCCGTACTA CACACCGCTC
    ATCCGCGTGG ACCGTCCTCT AAGAGACAGC GCCAGCAGCA GCCATTCATC CATCAGGAAG
    AGCTCGTGGT ACACAGACGA CCCTGAGTAC CAGCAGAGGA ACTTCTCACC TGTTCACCTG
    CAGGTCCCAC CCGAGTACAG GAACCAGTAC CTGCAGAAGA GAGGCAGCGC TACCAGCCTG
    GTGGAGGCGG TCTTGATATC AGAAGGACTG GGTCGATACG CCAAGGATCC CAAATTTGTG
    GCCGCCACAA AGCATGAGAT TGCGGACGCG TGCGAGATGA CCATTGACGA AATGGAAAGC
    GCCGCCAGCC ATCTTCTTAA CGGTGGCATT ACACCAGTAG TCAACGGAGT CAATGTGTTT
    CCCATACTGG GCCACAGAGA CTACGAGCTG CAGGACGTCT CGGCGAGCTA CAGCGACGAG
    GAGCCCGAAC CCGAGCCCAG ACCTCGTTAC GAGGAGGACC TTGCAGATGA AATGATATGC
    ATTACCACTT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335254.1
    CDS411..6665
    Translation

    Target ORF information:

    RefSeq Version XM_021479579.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479579.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCGGG TTACACTTGC CAGCCTCATG GAGAAGAAGA AGAAGGGGTT TGGCTGGTTC
    AGCCAGTCCT CTGACACTCA TGCGAGTATG CCGGCAAGTG AGACTGAATC CATGAATACA
    GAGAACGAGA AAGGAGAAGA TGAGAAGGCG ACATGTTGCG GCCCGACATG TCAAAAAATA
    TCAAAATCAA AGTTCAGTCG GCGCTGGCGT CGCTGGAACC GGTTGTGTCG GAGGAATTGT
    CGCTTGGCTG TAAAATCTGT GCCATTCTAT TGGCTGGTAA TCATCCTGGT GTTCCTGAAC
    ACTTTGACCA TCTCTTCAGA ACATTACAAC CAGCCCATGT GGCTCACACA AGTACAGGAT
    GTGGCTAATA AAGTGCTGCT GGCCATGTTC ACGTGTGAGA TGTTGGTGAA GATGTACAGT
    CTGGGCCTGC AGGCTTATTT TGTGTCTCTT TTTAATCGCT TTGACTGCTT TGTGGTGTGT
    GGAGGCATCA CTGAAACCAT CCTTGTGGAG TTTGAGATTA TGTCACCCCT CGGCATCTCA
    GTGTTTCGAT GTGTTCGACT TCTCAGGATC TTTAAAGTTA CACGCCACTG GGCGTCTCTC
    AGTAACCTGG TGGCATCTCT GCTGAACTCC ATGAAGTCCA TTGCTTCTCT ACTGCTGCTG
    CTCTTCCTCT TCATCATCAT CTTTAGCCTC CTCGGTATGC AGGTGTTCGG CGGCAAGTTT
    AACTTTGACG AGACACAAAC CAAGAGGAGC ACCTTTGACA ACTTCCCTCA GGCCTTGCTC
    ACTGTGTTTC AGATTCTAAC TGGTGAAGAC TGGAATGCCG TCATGTATGA CGGAATTATG
    GCGTACGGCG GCCCTTCGTC ATCAGGAATG ATTGTGTGCA TATACTTCAT CATCCTTTTC
    ATCTGTGGAA ACTACATTCT CCTGAATGTG TTCTTGGCTA TCGCTGTGGA CAATCTTGCT
    GATGCAGAGT CTCTGAACAC GGACGATACG AAGAAACCTG ATGAAATCGA CGAAATTGAA
    GATGAAGCCA AGGCTGGAGA GGAGGATGAG AAGGATAATG CCGAGGAGGA TGAAGAAGAG
    CCAGATGTTC CTGCAGGGCC TCGTCCAAAG ATCTCAGAGC TTGTTAAGAA GGAGAAGATC
    ACTCCAATCC CAGAGGGCAG TGCGTTCTTC ATCTTCAGCA ACACAAACCC AGTTCGAGTC
    GCCTGCCACA AGCTGATAAA CCACCACATC TTCACCAACC TCATCCTTGT GTTCATTATG
    CTAAGCTCAG CGTCATTGGC TGCTGAGGAT CCAATCAGAA ACTTCTCTGC TAGGAATATT
    ATACTTGGTT ACTTTGACTA TGCTTTCACG GCTATCTTTA CTGTAGAGAT CGTGTTAAAG
    ATGACCACGT ATGGCGCTTT TCTCCATAAG GGTGCTTTCT GCAGGAACTA CTTTAATCTT
    CTAGATCTGC TGGTGGTCGG AGTTTCGCTC GTGTCCTTCG GGATTCAATC ATCGGCCATC
    TCAGTGGTGA AGATTCTGAG GGTTCTGAGA GTTTTGCGTC CACTCAGAGC CATAAACAGA
    GCTAAAGGAC TAAAGCATGT GGTCCAGTGT GTGTTTGTGG CCATCAGGAC TATAGGAAAC
    ATCATGATCG TCACCACTCT GCTGCAGTTT ATGTTCGCCT GCATTGGTGT CCAGCTCTTC
    AAGGGAAAGT TTTACCGTTG TAACGATGAG GCCAAGTCCA GCCCAGAGGA GTGCAAGGGA
    ACGTACATCA TGTATAAGGA AGGAGACGTG AACCAGCCAA TCATTCAGAA GCGCCACTGG
    CACAACAGTG ATTTCAACTT TGACAATGTG CTCATGGCCA TGATGGCTCT GTTCACCGTG
    TCCACGTTTG AAGGCTGGCC AGCGTTGCTG TACAAGGCCA TCGACTCCAA CCGGGAGAAC
    ATGGGTCCCA TCTACAACTA CCGAGTGGAG ATTTCCATCT TCTTCATCAT CTACATCATC
    ATCATCGCTT TCTTTATGAT GAACATCTTT GTGGGTTTCG TCATCGTGAC CTTTCAGGAA
    CAGGGAGAGA AGGAGTACAA GAACTGTGAG CTGGACAAGA ACCAGCGTCA GTGTGTGGAG
    TATGCATTGA AAGCACGTCC TCTCAGGAGG TACATCCCAA AGAACCCATA TCAGTATAAG
    TTCTGGTATG TGGTGAACTC CACCGGCTTC GAGTACATCA TGTTTGTCCT GATTTTACTC
    AACACCATCT GTCTCGCCGT GCAGCACTAT GGTCAGTCTG AGCTCTTCAA TTATGTTATG
    GACATTCTGA ATATGGTCTT CACTGCCGTC TTCACTGTGG AGATGGTGCT CAAGCTGATC
    GCCTTCAAGC CTCGGCATTA TTTCACTGAC GCATGGAACA CATTTGACGC CTTAATTGTT
    GTCGGTAGCG TCGTAGATAT TGCTATCACT GAAGTCAACC CCACAGAGGC ACCTCAGGTG
    GATGAGTCGG GGAACACTGA GGACAGCGCT CGCATTTCCA TCACTTTCTT CCGTCTTTTT
    CGAGTGATGC GATTGGTCAA GCTGCTGAGC AGAGGGGAGG GAATTCGAAC CCTACTCTGG
    ACCTTTATCA AATCCTTCCA GGCACTGCCT TATGTTGCTT TGCTGATAGC CATGCTCTTC
    TTCATCTACG CTGTCATTGG CATGCAGGTG TTTGGGAAAA TTGCCATGGT GGATCACACT
    CAAATCAACA GGAACAACAA CTTTCAGACG TTCCCTCAGG CTGTGCTTCT GCTCTTCAGG
    TGTGCTACGG GTGAAGCCTG GCAGGAGATC ATGTTAGCAT GTATGCCAGG CAAGCTGTGT
    GACCCGGAGT CAGACTATAA TCCTGGGGAA GAAATGACCT GCGGGAGCAG TTTCGCCATC
    ATCTACTTCA TCACCTTCTA CATGCTCTGC GCCTTCCTGA TCATTAACCT GTTTGTGGCT
    GTCATCATGG ACAACTTCGA CTATCTGACC CGTGATTGGT CAATTCTGGG GCCACATCAC
    CTTGATGAGT TTAAGAGGAT TTGGTCAGAA TATGACCCAG AGGCCAAGGG CAGAATAAAA
    CATCTTGATG TAGTGACGTT GTTGCGCCGT ATTCAGCCTC CTCTCGGCTT TGGGAAACTC
    TGTCCTCATA GAGTCGCCTG CAAGAGGCTG GTGGCTATGA ACATGCCCCT GAACAGTGAC
    GGCACGGTCA TGTTTAACGC GACACTGTTT GCTCTCGTGA GAACCGCATT GAAAATCAAA
    ACAGAAGGTA ACCTAGAACA AGCGAATGAA GAGCTCCGAG CTGTGATTAA GAAGATCTGG
    AAGAGAACCA GCATGAAGCT TCTGGATCAA GTGGTTCCCC CTGCTGGTGA TGATGAGGTA
    ACGGTGGGGA AGTTCTATGC CACTTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA
    CGCAAAGAGG AAGGCCTGGT GGGAGTTCAT CCGGCACAGA ACAACACGGC CATCGCACTG
    CAGGCTGGCC TTCGTACGCT GCATGACATT GGGCCAGAAA TCCGCCGTGC GATATCATGT
    GACCTGCAGG ATGATGAGCT AGTAGACTTT ATACCAGAGG AGGACGAAGA AATTTATAGG
    CGAAACGGAG GACTCTTTGG CAACCACATC AACCACATAA ACGGAGACCC CCGGAGGAGC
    TCTGGCCACC AAACAAACGC CACACAGCGC CCCCTGCAGG TGCAGCCGCC CCCACATTAC
    GTCCACATGG AGCAGCCCGT GGGGCGTCTG GGTCGCGCCA ATGCCATGGC ACAGCAAAAC
    CATCATCGTC ACCACCACCA CCATCACCAT CATCATCATC ACAACAACTC CTACAACAAA
    TCGCCCAAGT CCACCAACAT CAACCTCAAC AACGCCAACG TGTCCAGTTT ACCCAATGGT
    GGACACAATC GCTACTACGA ACACGCTCCC GCTAATGGCT ACCCAGGGTC ATATTACGGA
    GAATACGACA AGCCCAGGAC CCCTCATGGG CAAAGATCGC AGGGCAGCGA CAGAAGACGA
    CCCACGATAC GCCGCGAAGA GGAGTATGAA GAAGATCGCT ACTCTGGAGA GTATTACAGC
    GGAGAGGAGT TTTATGAAGA TGACAGCATG CTATCGGGTG ATAGGTACCC CAACAGTGAC
    CAGGAGTACG AGACGCCGCG GGGTTACCAT CATCCTGACA GTTACTATGA AGATGATGAG
    CAGCCCCTTT ATCACGATTC ACATAGGTCA CCAAAGAGAA GACTGCTTCC TCCAACACCG
    CAAGGTAATC GGAGACCGTC GTTCAATTTT GAGTGTCTGC GCAGACAGAG CAGTCAAGAC
    GATCTTCCTC ACCAGCGAAC TGCTCTTCCG TTACATCTCA TGCAACACCA GGTGATGGCA
    GTGGCGGGTC TGGACTCTAG CAGGGCTCAC AGGCTTTCAC CAACTCGATC TACCAGATCA
    TGGGCTTCAC CTCCTCCAAC TCCTGCAAGT AAAGACCGGA CGCCGTACTA CACACCGCTC
    ATCCGCGTGG ACCGTCCTCT AAGAGACAGC GCCAGCAGCA GCCATTCATC CATCAGGAAG
    AGCTCGTGGT ACACAGACGA CCCTGAGTAC CAGCAGAGGA ACTTCTCACC TGTTCACCTG
    CAGGTCCCAC CCGAGTACAG GAACCAGTAC CTGCAGAAGA GAGGCAGCGC TACCAGCCTG
    GTGGAGGCGG TCTTGATATC AGAAGGACTG GGTCGATACG CCAAGGATCC CAAATTTGTG
    GCCGCCACAA AGCATGAGAT TGCGGACGCG TGCGAGATGA CCATTGACGA AATGGAAAGC
    GCCGCCAGCC ATCTTCTTAA CGGTGGCATT ACACCAGTAG TCAACGGAGT CAATGTGTTT
    CCCATACTGG GCCACAGAGA CTACGAGCTG CAGGACGTCT CGGCGAGCTA CAGCGACGAG
    GAGCCCGAAC CCGAGCCCAG ACCTCGTTAC GAGGAGGACC TTGCAGATGA AATGATATGC
    ATTACCACTT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147860
    Clone ID Related Accession (Same CDS sequence) XM_021479580.1
    Accession Version XM_021479580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCGGG TTACACTTGC CAGCCTCATG GAGAAGAAGA AGAAGGGGTT TGGCTGGTTC
    AGCCAGTCCT CTGACACTCA TGCGAGTATG CCGGCAAGTG AGACTGAATC CATGAATACA
    GAGAACGAGA AAGGAGAAGA TGAGAAGGCG ACATGTTGCG GCCCGACATG TCAAAAAATA
    TCAAAATCAA AGTTCAGTCG GCGCTGGCGT CGCTGGAACC GGTTGTGTCG GAGGAATTGT
    CGCTTGGCTG TAAAATCTGT GCCATTCTAT TGGCTGGTAA TCATCCTGGT GTTCCTGAAC
    ACTTTGACCA TCTCTTCAGA ACATTACAAC CAGCCCATGT GGCTCACACA AGTACAGGAT
    GTGGCTAATA AAGTGCTGCT GGCCATGTTC ACGTGTGAGA TGTTGGTGAA GATGTACAGT
    CTGGGCCTGC AGGCTTATTT TGTGTCTCTT TTTAATCGCT TTGACTGCTT TGTGGTGTGT
    GGAGGCATCA CTGAAACCAT CCTTGTGGAG TTTGAGATTA TGTCACCCCT CGGCATCTCA
    GTGTTTCGAT GTGTTCGACT TCTCAGGATC TTTAAAGTTA CACGCCACTG GGCGTCTCTC
    AGTAACCTGG TGGCATCTCT GCTGAACTCC ATGAAGTCCA TTGCTTCTCT ACTGCTGCTG
    CTCTTCCTCT TCATCATCAT CTTTAGCCTC CTCGGTATGC AGGTGTTCGG CGGCAAGTTT
    AACTTTGACG AGACACAAAC CAAGAGGAGC ACCTTTGACA ACTTCCCTCA GGCCTTGCTC
    ACTGTGTTTC AGATTCTAAC TGGTGAAGAC TGGAATGCCG TCATGTATGA CGGAATTATG
    GCGTACGGCG GCCCTTCGTC ATCAGGAATG ATTGTGTGCA TATACTTCAT CATCCTTTTC
    ATCTGTGGAA ACTACATTCT CCTGAATGTG TTCTTGGCTA TCGCTGTGGA CAATCTTGCT
    GATGCAGAGT CTCTGAACAC GGACGATACG AAGAAACCTG ATGAAATCGA CGAAATTGAA
    GATGAAGCCA AGGCTGGAGA GGAGGATGAG AAGGATAATG CCGAGGAGGA TGAAGAAGAG
    CCAGATGTTC CTGCAGGGCC TCGTCCAAAG ATCTCAGAGC TTGTTAAGAA GGAGAAGATC
    ACTCCAATCC CAGAGGGCAG TGCGTTCTTC ATCTTCAGCA ACACAAACCC AGTTCGAGTC
    GCCTGCCACA AGCTGATAAA CCACCACATC TTCACCAACC TCATCCTTGT GTTCATTATG
    CTAAGCTCAG CGTCATTGGC TGCTGAGGAT CCAATCAGAA ACTTCTCTGC TAGGAATATT
    GTCCTAGGCT ATGCAGATTA TGTCTTCACT AGTATGTTTA CATTTGAGAT CGTGTTAAAG
    ATGACCACGT ATGGCGCTTT TCTCCATAAG GGTGCTTTCT GCAGGAACTA CTTTAATCTT
    CTAGATCTGC TGGTGGTCGG AGTTTCGCTC GTGTCCTTCG GGATTCAATC ATCGGCCATC
    TCAGTGGTGA AGATTCTGAG GGTTCTGAGA GTTTTGCGTC CACTCAGAGC CATAAACAGA
    GCTAAAGGAC TAAAGCATGT GGTCCAGTGT GTGTTTGTGG CCATCAGGAC TATAGGAAAC
    ATCATGATCG TCACCACTCT GCTGCAGTTT ATGTTCGCCT GCATTGGTGT CCAGCTCTTC
    AAGGGAAAGT TTTACCGTTG TAACGATGAG GCCAAGTCCA GCCCAGAGGA GTGCAAGGGA
    ACGTACATCA TGTATAAGGA AGGAGACGTG AACCAGCCAA TCATTCAGAA GCGCCACTGG
    CACAACAGTG ATTTCAACTT TGACAATGTG CTCATGGCCA TGATGGCTCT GTTCACCGTG
    TCCACGTTTG AAGGCTGGCC AGCGTTGCTG TACAAGGCCA TCGACTCCAA CCGGGAGAAC
    ATGGGTCCCA TCTACAACTA CCGAGTGGAG ATTTCCATCT TCTTCATCAT CTACATCATC
    ATCATCGCTT TCTTTATGAT GAACATCTTT GTGGGTTTCG TCATCGTGAC CTTTCAGGAA
    CAGGGAGAGA AGGAGTACAA GAACTGTGAG CTGGACAAGA ACCAGCGTCA GTGTGTGGAG
    TATGCATTGA AAGCACGTCC TCTCAGGAGG TACATCCCAA AGAACCCATA TCAGTATAAG
    TTCTGGTATG TGGTGAACTC CACCGGCTTC GAGTACATCA TGTTTGTCCT GATTTTACTC
    AACACCATCT GTCTCGCCGT GCAGCACTAT GGTCAGTCTG AGCTCTTCAA TTATGTTATG
    GACATTCTGA ATATGGTCTT CACTGCCGTC TTCACTGTGG AGATGGTGCT CAAGCTGATC
    GCCTTCAAGC CTCGGCATTA TTTCACTGAC GCATGGAACA CATTTGACGC CTTAATTGTT
    GTCGGTAGCG TCGTAGATAT TGCTATCACT GAAGTCAACA ACACTGAGGA CAGCGCTCGC
    ATTTCCATCA CTTTCTTCCG TCTTTTTCGA GTGATGCGAT TGGTCAAGCT GCTGAGCAGA
    GGGGAGGGAA TTCGAACCCT ACTCTGGACC TTTATCAAAT CCTTCCAGGC ACTGCCTTAT
    GTTGCTTTGC TGATAGCCAT GCTCTTCTTC ATCTACGCTG TCATTGGCAT GCAGGTGTTT
    GGGAAAATTG CCATGGTGGA TCACACTCAA ATCAACAGGA ACAACAACTT TCAGACGTTC
    CCTCAGGCTG TGCTTCTGCT CTTCAGGTGT GCTACGGGTG AAGCCTGGCA GGAGATCATG
    TTAGCATGTA TGCCAGGCAA GCTGTGTGAC CCGGAGTCAG ACTATAATCC TGGGGAAGAA
    ATGACCTGCG GGAGCAGTTT CGCCATCATC TACTTCATCA CCTTCTACAT GCTCTGCGCC
    TTCCTGATCA TTAACCTGTT TGTGGCTGTC ATCATGGACA ACTTCGACTA TCTGACCCGT
    GATTGGTCAA TTCTGGGGCC ACATCACCTT GATGAGTTTA AGAGGATTTG GTCAGAATAT
    GACCCAGAGG CCAAGGGCAG AATAAAACAT CTTGATGTAG TGACGTTGTT GCGCCGTATT
    CAGCCTCCTC TCGGCTTTGG GAAACTCTGT CCTCATAGAG TCGCCTGCAA GAGGCTGGTG
    GCTATGAACA TGCCCCTGAA CAGTGACGGC ACGGTCATGT TTAACGCGAC ACTGTTTGCT
    CTCGTGAGAA CCGCATTGAA AATCAAAACA GAAGGTAACC TAGAACAAGC GAATGAAGAG
    CTCCGAGCTG TGATTAAGAA GATCTGGAAG AGAACCAGCA TGAAGCTTCT GGATCAAGTG
    GTTCCCCCTG CTGGTGATGA TGAGGTAACG GTGGGGAAGT TCTATGCCAC TTTCCTGATA
    CAGGACTACT TTAGGAAATT CAAGAAACGC AAAGAGGAAG GCCTGGTGGG AGTTCATCCG
    GCACAGAACA ACACGGCCAT CGCACTGCAG GCTGGCCTTC GTACGCTGCA TGACATTGGG
    CCAGAAATCC GCCGTGCGAT ATCATGTGAC CTGCAGGATG ATGAGCTAGT AGACTTTATA
    CCAGAGGAGG ACGAAGAAAT TTATAGGCGA AACGGAGGAC TCTTTGGCAA CCACATCAAC
    CACATAAACG GAGACCCCCG GAGGAGCTCT GGCCACCAAA CAAACGCCAC ACAGCGCCCC
    CTGCAGGTGC AGCCGCCCCC ACATTACGTC CACATGGAGC AGCCCGTGGG GCGTCTGGGT
    CGCGCCAATG CCATGGCACA GCAAAACCAT CATCGTCACC ACCACCACCA TCACCATCAT
    CATCATCACA ACAACTCCTA CAACAAATCG CCCAAGTCCA CCAACATCAA CCTCAACAAC
    GCCAACGTGT CCAGTTTACC CAATGGTGGA CACAATCGCT ACTACGAACA CGCTCCCGCT
    AATGGCTACC CAGGGTCATA TTACGGAGAA TACGACAAGC CCAGGACCCC TCATGGGCAA
    AGAAGGCGCT ACTATGAGAC ATATATTAGA TCGCAGGGCA GCGACAGAAG ACGACCCACG
    ATACGCCGCG AAGAGGAGTA TGAAGAAGAT CGCTACTCTG GAGAGTATTA CAGCGGAGAG
    GAGTTTTATG AAGATGACAG CATGCTATCG GGTGATAGGT ACCCCAACAG TGACCAGGAG
    TACGAGACGC CGCGGGGTTA CCATCATCCT GACAGTTACT ATGAAGATGA TGAGCAGCCC
    CTTTATCACG ATTCACATAG GTCACCAAAG AGAAGACTGC TTCCTCCAAC ACCGCAAGGT
    AATCGGAGAC CGTCGTTCAA TTTTGAGTGT CTGCGCAGAC AGAGCAGTCA AGACGATCTT
    CCTCACCAGC GAACTGCTCT TCCGTTACAT CTCATGCAAC ACCAGGTGAT GGCAGTGGCG
    GGTCTGGACT CTAGCAGGGC TCACAGGCTT TCACCAACTC GATCTACCAG ATCATGGGCT
    TCACCTCCTC CAACTCCTGC AAGTAAAGAC CGGACGCCGT ACTACACACC GCTCATCCGC
    GTGGACCGTC CTCTAAGAGA CAGCGCCAGC AGCAGCCATT CATCCATCAG GAAGAGCTCG
    TGGTACACAG ACGACCCTGA GTACCAGCAG AGGAACTTCT CACCTGTTCA CCTGCAGGTC
    CCACCCGAGT ACAGGAACCA GTACCTGCAG AAGAGAGGCA GCGCTACCAG CCTGGTGGAG
    GCGGTCTTGA TATCAGAAGG ACTGGGTCGA TACGCCAAGG ATCCCAAATT TGTGGCCGCC
    ACAAAGCATG AGATTGCGGA CGCGTGCGAG ATGACCATTG ACGAAATGGA AAGCGCCGCC
    AGCCATCTTC TTAACGGTGG CATTACACCA GTAGTCAACG GAGTCAATGT GTTTCCCATA
    CTGGGCCACA GAGACTACGA GCTGCAGGAC GTCTCGGCGA GCTACAGCGA CGAGGAGCCC
    GAACCCGAGC CCAGACCTCG TTACGAGGAG GACCTTGCAG ATGAAATGAT ATGCATTACC
    ACTTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335255.1
    CDS410..6658
    Translation

    Target ORF information:

    RefSeq Version XM_021479580.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCGGG TTACACTTGC CAGCCTCATG GAGAAGAAGA AGAAGGGGTT TGGCTGGTTC
    AGCCAGTCCT CTGACACTCA TGCGAGTATG CCGGCAAGTG AGACTGAATC CATGAATACA
    GAGAACGAGA AAGGAGAAGA TGAGAAGGCG ACATGTTGCG GCCCGACATG TCAAAAAATA
    TCAAAATCAA AGTTCAGTCG GCGCTGGCGT CGCTGGAACC GGTTGTGTCG GAGGAATTGT
    CGCTTGGCTG TAAAATCTGT GCCATTCTAT TGGCTGGTAA TCATCCTGGT GTTCCTGAAC
    ACTTTGACCA TCTCTTCAGA ACATTACAAC CAGCCCATGT GGCTCACACA AGTACAGGAT
    GTGGCTAATA AAGTGCTGCT GGCCATGTTC ACGTGTGAGA TGTTGGTGAA GATGTACAGT
    CTGGGCCTGC AGGCTTATTT TGTGTCTCTT TTTAATCGCT TTGACTGCTT TGTGGTGTGT
    GGAGGCATCA CTGAAACCAT CCTTGTGGAG TTTGAGATTA TGTCACCCCT CGGCATCTCA
    GTGTTTCGAT GTGTTCGACT TCTCAGGATC TTTAAAGTTA CACGCCACTG GGCGTCTCTC
    AGTAACCTGG TGGCATCTCT GCTGAACTCC ATGAAGTCCA TTGCTTCTCT ACTGCTGCTG
    CTCTTCCTCT TCATCATCAT CTTTAGCCTC CTCGGTATGC AGGTGTTCGG CGGCAAGTTT
    AACTTTGACG AGACACAAAC CAAGAGGAGC ACCTTTGACA ACTTCCCTCA GGCCTTGCTC
    ACTGTGTTTC AGATTCTAAC TGGTGAAGAC TGGAATGCCG TCATGTATGA CGGAATTATG
    GCGTACGGCG GCCCTTCGTC ATCAGGAATG ATTGTGTGCA TATACTTCAT CATCCTTTTC
    ATCTGTGGAA ACTACATTCT CCTGAATGTG TTCTTGGCTA TCGCTGTGGA CAATCTTGCT
    GATGCAGAGT CTCTGAACAC GGACGATACG AAGAAACCTG ATGAAATCGA CGAAATTGAA
    GATGAAGCCA AGGCTGGAGA GGAGGATGAG AAGGATAATG CCGAGGAGGA TGAAGAAGAG
    CCAGATGTTC CTGCAGGGCC TCGTCCAAAG ATCTCAGAGC TTGTTAAGAA GGAGAAGATC
    ACTCCAATCC CAGAGGGCAG TGCGTTCTTC ATCTTCAGCA ACACAAACCC AGTTCGAGTC
    GCCTGCCACA AGCTGATAAA CCACCACATC TTCACCAACC TCATCCTTGT GTTCATTATG
    CTAAGCTCAG CGTCATTGGC TGCTGAGGAT CCAATCAGAA ACTTCTCTGC TAGGAATATT
    GTCCTAGGCT ATGCAGATTA TGTCTTCACT AGTATGTTTA CATTTGAGAT CGTGTTAAAG
    ATGACCACGT ATGGCGCTTT TCTCCATAAG GGTGCTTTCT GCAGGAACTA CTTTAATCTT
    CTAGATCTGC TGGTGGTCGG AGTTTCGCTC GTGTCCTTCG GGATTCAATC ATCGGCCATC
    TCAGTGGTGA AGATTCTGAG GGTTCTGAGA GTTTTGCGTC CACTCAGAGC CATAAACAGA
    GCTAAAGGAC TAAAGCATGT GGTCCAGTGT GTGTTTGTGG CCATCAGGAC TATAGGAAAC
    ATCATGATCG TCACCACTCT GCTGCAGTTT ATGTTCGCCT GCATTGGTGT CCAGCTCTTC
    AAGGGAAAGT TTTACCGTTG TAACGATGAG GCCAAGTCCA GCCCAGAGGA GTGCAAGGGA
    ACGTACATCA TGTATAAGGA AGGAGACGTG AACCAGCCAA TCATTCAGAA GCGCCACTGG
    CACAACAGTG ATTTCAACTT TGACAATGTG CTCATGGCCA TGATGGCTCT GTTCACCGTG
    TCCACGTTTG AAGGCTGGCC AGCGTTGCTG TACAAGGCCA TCGACTCCAA CCGGGAGAAC
    ATGGGTCCCA TCTACAACTA CCGAGTGGAG ATTTCCATCT TCTTCATCAT CTACATCATC
    ATCATCGCTT TCTTTATGAT GAACATCTTT GTGGGTTTCG TCATCGTGAC CTTTCAGGAA
    CAGGGAGAGA AGGAGTACAA GAACTGTGAG CTGGACAAGA ACCAGCGTCA GTGTGTGGAG
    TATGCATTGA AAGCACGTCC TCTCAGGAGG TACATCCCAA AGAACCCATA TCAGTATAAG
    TTCTGGTATG TGGTGAACTC CACCGGCTTC GAGTACATCA TGTTTGTCCT GATTTTACTC
    AACACCATCT GTCTCGCCGT GCAGCACTAT GGTCAGTCTG AGCTCTTCAA TTATGTTATG
    GACATTCTGA ATATGGTCTT CACTGCCGTC TTCACTGTGG AGATGGTGCT CAAGCTGATC
    GCCTTCAAGC CTCGGCATTA TTTCACTGAC GCATGGAACA CATTTGACGC CTTAATTGTT
    GTCGGTAGCG TCGTAGATAT TGCTATCACT GAAGTCAACA ACACTGAGGA CAGCGCTCGC
    ATTTCCATCA CTTTCTTCCG TCTTTTTCGA GTGATGCGAT TGGTCAAGCT GCTGAGCAGA
    GGGGAGGGAA TTCGAACCCT ACTCTGGACC TTTATCAAAT CCTTCCAGGC ACTGCCTTAT
    GTTGCTTTGC TGATAGCCAT GCTCTTCTTC ATCTACGCTG TCATTGGCAT GCAGGTGTTT
    GGGAAAATTG CCATGGTGGA TCACACTCAA ATCAACAGGA ACAACAACTT TCAGACGTTC
    CCTCAGGCTG TGCTTCTGCT CTTCAGGTGT GCTACGGGTG AAGCCTGGCA GGAGATCATG
    TTAGCATGTA TGCCAGGCAA GCTGTGTGAC CCGGAGTCAG ACTATAATCC TGGGGAAGAA
    ATGACCTGCG GGAGCAGTTT CGCCATCATC TACTTCATCA CCTTCTACAT GCTCTGCGCC
    TTCCTGATCA TTAACCTGTT TGTGGCTGTC ATCATGGACA ACTTCGACTA TCTGACCCGT
    GATTGGTCAA TTCTGGGGCC ACATCACCTT GATGAGTTTA AGAGGATTTG GTCAGAATAT
    GACCCAGAGG CCAAGGGCAG AATAAAACAT CTTGATGTAG TGACGTTGTT GCGCCGTATT
    CAGCCTCCTC TCGGCTTTGG GAAACTCTGT CCTCATAGAG TCGCCTGCAA GAGGCTGGTG
    GCTATGAACA TGCCCCTGAA CAGTGACGGC ACGGTCATGT TTAACGCGAC ACTGTTTGCT
    CTCGTGAGAA CCGCATTGAA AATCAAAACA GAAGGTAACC TAGAACAAGC GAATGAAGAG
    CTCCGAGCTG TGATTAAGAA GATCTGGAAG AGAACCAGCA TGAAGCTTCT GGATCAAGTG
    GTTCCCCCTG CTGGTGATGA TGAGGTAACG GTGGGGAAGT TCTATGCCAC TTTCCTGATA
    CAGGACTACT TTAGGAAATT CAAGAAACGC AAAGAGGAAG GCCTGGTGGG AGTTCATCCG
    GCACAGAACA ACACGGCCAT CGCACTGCAG GCTGGCCTTC GTACGCTGCA TGACATTGGG
    CCAGAAATCC GCCGTGCGAT ATCATGTGAC CTGCAGGATG ATGAGCTAGT AGACTTTATA
    CCAGAGGAGG ACGAAGAAAT TTATAGGCGA AACGGAGGAC TCTTTGGCAA CCACATCAAC
    CACATAAACG GAGACCCCCG GAGGAGCTCT GGCCACCAAA CAAACGCCAC ACAGCGCCCC
    CTGCAGGTGC AGCCGCCCCC ACATTACGTC CACATGGAGC AGCCCGTGGG GCGTCTGGGT
    CGCGCCAATG CCATGGCACA GCAAAACCAT CATCGTCACC ACCACCACCA TCACCATCAT
    CATCATCACA ACAACTCCTA CAACAAATCG CCCAAGTCCA CCAACATCAA CCTCAACAAC
    GCCAACGTGT CCAGTTTACC CAATGGTGGA CACAATCGCT ACTACGAACA CGCTCCCGCT
    AATGGCTACC CAGGGTCATA TTACGGAGAA TACGACAAGC CCAGGACCCC TCATGGGCAA
    AGAAGGCGCT ACTATGAGAC ATATATTAGA TCGCAGGGCA GCGACAGAAG ACGACCCACG
    ATACGCCGCG AAGAGGAGTA TGAAGAAGAT CGCTACTCTG GAGAGTATTA CAGCGGAGAG
    GAGTTTTATG AAGATGACAG CATGCTATCG GGTGATAGGT ACCCCAACAG TGACCAGGAG
    TACGAGACGC CGCGGGGTTA CCATCATCCT GACAGTTACT ATGAAGATGA TGAGCAGCCC
    CTTTATCACG ATTCACATAG GTCACCAAAG AGAAGACTGC TTCCTCCAAC ACCGCAAGGT
    AATCGGAGAC CGTCGTTCAA TTTTGAGTGT CTGCGCAGAC AGAGCAGTCA AGACGATCTT
    CCTCACCAGC GAACTGCTCT TCCGTTACAT CTCATGCAAC ACCAGGTGAT GGCAGTGGCG
    GGTCTGGACT CTAGCAGGGC TCACAGGCTT TCACCAACTC GATCTACCAG ATCATGGGCT
    TCACCTCCTC CAACTCCTGC AAGTAAAGAC CGGACGCCGT ACTACACACC GCTCATCCGC
    GTGGACCGTC CTCTAAGAGA CAGCGCCAGC AGCAGCCATT CATCCATCAG GAAGAGCTCG
    TGGTACACAG ACGACCCTGA GTACCAGCAG AGGAACTTCT CACCTGTTCA CCTGCAGGTC
    CCACCCGAGT ACAGGAACCA GTACCTGCAG AAGAGAGGCA GCGCTACCAG CCTGGTGGAG
    GCGGTCTTGA TATCAGAAGG ACTGGGTCGA TACGCCAAGG ATCCCAAATT TGTGGCCGCC
    ACAAAGCATG AGATTGCGGA CGCGTGCGAG ATGACCATTG ACGAAATGGA AAGCGCCGCC
    AGCCATCTTC TTAACGGTGG CATTACACCA GTAGTCAACG GAGTCAATGT GTTTCCCATA
    CTGGGCCACA GAGACTACGA GCTGCAGGAC GTCTCGGCGA GCTACAGCGA CGAGGAGCCC
    GAACCCGAGC CCAGACCTCG TTACGAGGAG GACCTTGCAG ATGAAATGAT ATGCATTACC
    ACTTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147861
    Clone ID Related Accession (Same CDS sequence) XM_021479581.1
    Accession Version XM_021479581.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCCGA GTATGCCGGC AAGTGAGACT GAATCCATGA ATACAGAGAA CGAGAAAGGA
    GAAGATGAGA AGGCGACATG TTGCGGCCCG ACATGTCAAA AAATATCAAA ATCAAAGTTC
    AGTCGGCGCT GGCGTCGCTG GAACCGGTTG TGTCGGAGGA ATTGTCGCTT GGCTGTAAAA
    TCTGTGCCAT TCTATTGGCT GGTAATCATC CTGGTGTTCC TGAACACTTT GACCATCTCT
    TCAGAACATT ACAACCAGCC CATGTGGCTC ACACAAGTAC AGGATGTGGC TAATAAAGTG
    CTGCTGGCCA TGTTCACGTG TGAGATGTTG GTGAAGATGT ACAGTCTGGG CCTGCAGGCT
    TATTTTGTGT CTCTTTTTAA TCGCTTTGAC TGCTTTGTGG TGTGTGGAGG CATCACTGAA
    ACCATCCTTG TGGAGTTTGA GATTATGTCA CCCCTCGGCA TCTCAGTGTT TCGATGTGTT
    CGACTTCTCA GGATCTTTAA AGTTACACGC CACTGGGCGT CTCTCAGTAA CCTGGTGGCA
    TCTCTGCTGA ACTCCATGAA GTCCATTGCT TCTCTACTGC TGCTGCTCTT CCTCTTCATC
    ATCATCTTTA GCCTCCTCGG TATGCAGGTG TTCGGCGGCA AGTTTAACTT TGACGAGACA
    CAAACCAAGA GGAGCACCTT TGACAACTTC CCTCAGGCCT TGCTCACTGT GTTTCAGATT
    CTAACTGGTG AAGACTGGAA TGCCGTCATG TATGACGGAA TTATGGCGTA CGGCGGCCCT
    TCGTCATCAG GAATGATTGT GTGCATATAC TTCATCATCC TTTTCATCTG TGGAAACTAC
    ATTCTCCTGA ATGTGTTCTT GGCTATCGCT GTGGACAATC TTGCTGATGC AGAGTCTCTG
    AACACGGACG ATACGAAGAA ACCTGATGAA ATCGACGAAA TTGAAGATGA AGCCAAGGCT
    GGAGAGGAGG ATGAGAAGGA TAATGCCGAG GAGGATGAAG AAGAGCCAGA TGTTCCTGCA
    GGGCCTCGTC CAAAGATCTC AGAGCTTGTT AAGAAGGAGA AGATCACTCC AATCCCAGAG
    GGCAGTGCGT TCTTCATCTT CAGCAACACA AACCCAGTTC GAGTCGCCTG CCACAAGCTG
    ATAAACCACC ACATCTTCAC CAACCTCATC CTTGTGTTCA TTATGCTAAG CTCAGCGTCA
    TTGGCTGCTG AGGATCCAAT CAGAAACTTC TCTGCTAGGA ATATTATACT TGGTTACTTT
    GACTATGCTT TCACGGCTAT CTTTACTGTA GAGATCGTGT TAAAGATGAC CACGTATGGC
    GCTTTTCTCC ATAAGGGTGC TTTCTGCAGG AACTACTTTA ATCTTCTAGA TCTGCTGGTG
    GTCGGAGTTT CGCTCGTGTC CTTCGGGATT CAATCATCGG CCATCTCAGT GGTGAAGATT
    CTGAGGGTTC TGAGAGTTTT GCGTCCACTC AGAGCCATAA ACAGAGCTAA AGGACTAAAG
    CATGTGGTCC AGTGTGTGTT TGTGGCCATC AGGACTATAG GAAACATCAT GATCGTCACC
    ACTCTGCTGC AGTTTATGTT CGCCTGCATT GGTGTCCAGC TCTTCAAGGG AAAGTTTTAC
    CGTTGTAACG ATGAGGCCAA GTCCAGCCCA GAGGAGTGCA AGGGAACGTA CATCATGTAT
    AAGGAAGGAG ACGTGAACCA GCCAATCATT CAGAAGCGCC ACTGGCACAA CAGTGATTTC
    AACTTTGACA ATGTGCTCAT GGCCATGATG GCTCTGTTCA CCGTGTCCAC GTTTGAAGGC
    TGGCCAGCGT TGCTGTACAA GGCCATCGAC TCCAACCGGG AGAACATGGG TCCCATCTAC
    AACTACCGAG TGGAGATTTC CATCTTCTTC ATCATCTACA TCATCATCAT CGCTTTCTTT
    ATGATGAACA TCTTTGTGGG TTTCGTCATC GTGACCTTTC AGGAACAGGG AGAGAAGGAG
    TACAAGAACT GTGAGCTGGA CAAGAACCAG CGTCAGTGTG TGGAGTATGC ATTGAAAGCA
    CGTCCTCTCA GGAGGTACAT CCCAAAGAAC CCATATCAGT ATAAGTTCTG GTATGTGGTG
    AACTCCACCG GCTTCGAGTA CATCATGTTT GTCCTGATTT TACTCAACAC CATCTGTCTC
    GCCGTGCAGC ACTATGGTCA GTCTGAGCTC TTCAATTATG TTATGGACAT TCTGAATATG
    GTCTTCACTG CCGTCTTCAC TGTGGAGATG GTGCTCAAGC TGATCGCCTT CAAGCCTCGG
    CATTATTTCA CTGACGCATG GAACACATTT GACGCCTTAA TTGTTGTCGG TAGCGTCGTA
    GATATTGCTA TCACTGAAGT CAACCCCACA GAGGCACCTC AGGTGGATGA GTCGGGGAAC
    ACTGAGGACA GCGCTCGCAT TTCCATCACT TTCTTCCGTC TTTTTCGAGT GATGCGATTG
    GTCAAGCTGC TGAGCAGAGG GGAGGGAATT CGAACCCTAC TCTGGACCTT TATCAAATCC
    TTCCAGGCAC TGCCTTATGT TGCTTTGCTG ATAGCCATGC TCTTCTTCAT CTACGCTGTC
    ATTGGCATGC AGGTGTTTGG GAAAATTGCC ATGGTGGATC ACACTCAAAT CAACAGGAAC
    AACAACTTTC AGACGTTCCC TCAGGCTGTG CTTCTGCTCT TCAGGTGTGC TACGGGTGAA
    GCCTGGCAGG AGATCATGTT AGCATGTATG CCAGGCAAGC TGTGTGACCC GGAGTCAGAC
    TATAATCCTG GGGAAGAAAT GACCTGCGGG AGCAGTTTCG CCATCATCTA CTTCATCACC
    TTCTACATGC TCTGCGCCTT CCTGATCATT AACCTGTTTG TGGCTGTCAT CATGGACAAC
    TTCGACTATC TGACCCGTGA TTGGTCAATT CTGGGGCCAC ATCACCTTGA TGAGTTTAAG
    AGGATTTGGT CAGAATATGA CCCAGAGGCC AAGGGCAGAA TAAAACATCT TGATGTAGTG
    ACGTTGTTGC GCCGTATTCA GCCTCCTCTC GGCTTTGGGA AACTCTGTCC TCATAGAGTC
    GCCTGCAAGA GGCTGGTGGC TATGAACATG CCCCTGAACA GTGACGGCAC GGTCATGTTT
    AACGCGACAC TGTTTGCTCT CGTGAGAACC GCATTGAAAA TCAAAACAGA AGGTAACCTA
    GAACAAGCGA ATGAAGAGCT CCGAGCTGTG ATTAAGAAGA TCTGGAAGAG AACCAGCATG
    AAGCTTCTGG ATCAAGTGGT TCCCCCTGCT GGTGATGATG AGGTAACGGT GGGGAAGTTC
    TATGCCACTT TCCTGATACA GGACTACTTT AGGAAATTCA AGAAACGCAA AGAGGAAGGC
    CTGGTGGGAG TTCATCCGGC ACAGAACAAC ACGGCCATCG CACTGCAGGC TGGCCTTCGT
    ACGCTGCATG ACATTGGGCC AGAAATCCGC CGTGCGATAT CATGTGACCT GCAGGATGAT
    GAGCTAGTAG ACTTTATACC AGAGGAGGAC GAAGAAATTT ATAGGCGAAA CGGAGGACTC
    TTTGGCAACC ACATCAACCA CATAAACGGA GACCCCCGGA GGAGCTCTGG CCACCAAACA
    AACGCCACAC AGCGCCCCCT GCAGGTGCAG CCGCCCCCAC ATTACGTCCA CATGGAGCAG
    CCCGTGGGGC GTCTGGGTCG CGCCAATGCC ATGGCACAGC AAAACCATCA TCGTCACCAC
    CACCACCATC ACCATCATCA TCATCACAAC AACTCCTACA ACAAATCGCC CAAGTCCACC
    AACATCAACC TCAACAACGC CAACGTGTCC AGTTTACCCA ATGGTGGACA CAATCGCTAC
    TACGAACACG CTCCCGCTAA TGGCTACCCA GGGTCATATT ACGGAGAATA CGACAAGCCC
    AGGACCCCTC ATGGGCAAAG AAGGCGCTAC TATGAGACAT ATATTAGATC GCAGGGCAGC
    GACAGAAGAC GACCCACGAT ACGCCGCGAA GAGGAGTATG AAGAAGATCG CTACTCTGGA
    GAGTATTACA GCGGAGAGGA GTTTTATGAA GATGACAGCA TGCTATCGGG TGATAGGTAC
    CCCAACAGTG ACCAGGAGTA CGAGACGCCG CGGGGTTACC ATCATCCTGA CAGTTACTAT
    GAAGATGATG AGCAGCCCCT TTATCACGAT TCACATAGGT CACCAAAGAG AAGACTGCTT
    CCTCCAACAC CGCAAGGTAA TCGGAGACCG TCGTTCAATT TTGAGTGTCT GCGCAGACAG
    AGCAGTCAAG ACGATCTTCC TCACCAGCGA ACTGCTCTTC CGTTACATCT CATGCAACAC
    CAGGTGATGG CAGTGGCGGG TCTGGACTCT AGCAGGGCTC ACAGGCTTTC ACCAACTCGA
    TCTACCAGAT CATGGGCTTC ACCTCCTCCA ACTCCTGCAA GTAAAGACCG GACGCCGTAC
    TACACACCGC TCATCCGCGT GGACCGTCCT CTAAGAGACA GCGCCAGCAG CAGCCATTCA
    TCCATCAGGA AGAGCTCGTG GTACACAGAC GACCCTGAGT ACCAGCAGAG GAACTTCTCA
    CCTGTTCACC TGCAGGTCCC ACCCGAGTAC AGGAACCAGT ACCTGCAGAA GAGAGGCAGC
    GCTACCAGCC TGGTGGAGGC GGTCTTGATA TCAGAAGGAC TGGGTCGATA CGCCAAGGAT
    CCCAAATTTG TGGCCGCCAC AAAGCATGAG ATTGCGGACG CGTGCGAGAT GACCATTGAC
    GAAATGGAAA GCGCCGCCAG CCATCTTCTT AACGGTGGCA TTACACCAGT AGTCAACGGA
    GTCAATGTGT TTCCCATACT GGGCCACAGA GACTACGAGC TGCAGGACGT CTCGGCGAGC
    TACAGCGACG AGGAGCCCGA ACCCGAGCCC AGACCTCGTT ACGAGGAGGA CCTTGCAGAT
    GAAATGATAT GCATTACCAC TTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335256.1
    CDS410..6616
    Translation

    Target ORF information:

    RefSeq Version XM_021479581.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479581.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCCGA GTATGCCGGC AAGTGAGACT GAATCCATGA ATACAGAGAA CGAGAAAGGA
    GAAGATGAGA AGGCGACATG TTGCGGCCCG ACATGTCAAA AAATATCAAA ATCAAAGTTC
    AGTCGGCGCT GGCGTCGCTG GAACCGGTTG TGTCGGAGGA ATTGTCGCTT GGCTGTAAAA
    TCTGTGCCAT TCTATTGGCT GGTAATCATC CTGGTGTTCC TGAACACTTT GACCATCTCT
    TCAGAACATT ACAACCAGCC CATGTGGCTC ACACAAGTAC AGGATGTGGC TAATAAAGTG
    CTGCTGGCCA TGTTCACGTG TGAGATGTTG GTGAAGATGT ACAGTCTGGG CCTGCAGGCT
    TATTTTGTGT CTCTTTTTAA TCGCTTTGAC TGCTTTGTGG TGTGTGGAGG CATCACTGAA
    ACCATCCTTG TGGAGTTTGA GATTATGTCA CCCCTCGGCA TCTCAGTGTT TCGATGTGTT
    CGACTTCTCA GGATCTTTAA AGTTACACGC CACTGGGCGT CTCTCAGTAA CCTGGTGGCA
    TCTCTGCTGA ACTCCATGAA GTCCATTGCT TCTCTACTGC TGCTGCTCTT CCTCTTCATC
    ATCATCTTTA GCCTCCTCGG TATGCAGGTG TTCGGCGGCA AGTTTAACTT TGACGAGACA
    CAAACCAAGA GGAGCACCTT TGACAACTTC CCTCAGGCCT TGCTCACTGT GTTTCAGATT
    CTAACTGGTG AAGACTGGAA TGCCGTCATG TATGACGGAA TTATGGCGTA CGGCGGCCCT
    TCGTCATCAG GAATGATTGT GTGCATATAC TTCATCATCC TTTTCATCTG TGGAAACTAC
    ATTCTCCTGA ATGTGTTCTT GGCTATCGCT GTGGACAATC TTGCTGATGC AGAGTCTCTG
    AACACGGACG ATACGAAGAA ACCTGATGAA ATCGACGAAA TTGAAGATGA AGCCAAGGCT
    GGAGAGGAGG ATGAGAAGGA TAATGCCGAG GAGGATGAAG AAGAGCCAGA TGTTCCTGCA
    GGGCCTCGTC CAAAGATCTC AGAGCTTGTT AAGAAGGAGA AGATCACTCC AATCCCAGAG
    GGCAGTGCGT TCTTCATCTT CAGCAACACA AACCCAGTTC GAGTCGCCTG CCACAAGCTG
    ATAAACCACC ACATCTTCAC CAACCTCATC CTTGTGTTCA TTATGCTAAG CTCAGCGTCA
    TTGGCTGCTG AGGATCCAAT CAGAAACTTC TCTGCTAGGA ATATTATACT TGGTTACTTT
    GACTATGCTT TCACGGCTAT CTTTACTGTA GAGATCGTGT TAAAGATGAC CACGTATGGC
    GCTTTTCTCC ATAAGGGTGC TTTCTGCAGG AACTACTTTA ATCTTCTAGA TCTGCTGGTG
    GTCGGAGTTT CGCTCGTGTC CTTCGGGATT CAATCATCGG CCATCTCAGT GGTGAAGATT
    CTGAGGGTTC TGAGAGTTTT GCGTCCACTC AGAGCCATAA ACAGAGCTAA AGGACTAAAG
    CATGTGGTCC AGTGTGTGTT TGTGGCCATC AGGACTATAG GAAACATCAT GATCGTCACC
    ACTCTGCTGC AGTTTATGTT CGCCTGCATT GGTGTCCAGC TCTTCAAGGG AAAGTTTTAC
    CGTTGTAACG ATGAGGCCAA GTCCAGCCCA GAGGAGTGCA AGGGAACGTA CATCATGTAT
    AAGGAAGGAG ACGTGAACCA GCCAATCATT CAGAAGCGCC ACTGGCACAA CAGTGATTTC
    AACTTTGACA ATGTGCTCAT GGCCATGATG GCTCTGTTCA CCGTGTCCAC GTTTGAAGGC
    TGGCCAGCGT TGCTGTACAA GGCCATCGAC TCCAACCGGG AGAACATGGG TCCCATCTAC
    AACTACCGAG TGGAGATTTC CATCTTCTTC ATCATCTACA TCATCATCAT CGCTTTCTTT
    ATGATGAACA TCTTTGTGGG TTTCGTCATC GTGACCTTTC AGGAACAGGG AGAGAAGGAG
    TACAAGAACT GTGAGCTGGA CAAGAACCAG CGTCAGTGTG TGGAGTATGC ATTGAAAGCA
    CGTCCTCTCA GGAGGTACAT CCCAAAGAAC CCATATCAGT ATAAGTTCTG GTATGTGGTG
    AACTCCACCG GCTTCGAGTA CATCATGTTT GTCCTGATTT TACTCAACAC CATCTGTCTC
    GCCGTGCAGC ACTATGGTCA GTCTGAGCTC TTCAATTATG TTATGGACAT TCTGAATATG
    GTCTTCACTG CCGTCTTCAC TGTGGAGATG GTGCTCAAGC TGATCGCCTT CAAGCCTCGG
    CATTATTTCA CTGACGCATG GAACACATTT GACGCCTTAA TTGTTGTCGG TAGCGTCGTA
    GATATTGCTA TCACTGAAGT CAACCCCACA GAGGCACCTC AGGTGGATGA GTCGGGGAAC
    ACTGAGGACA GCGCTCGCAT TTCCATCACT TTCTTCCGTC TTTTTCGAGT GATGCGATTG
    GTCAAGCTGC TGAGCAGAGG GGAGGGAATT CGAACCCTAC TCTGGACCTT TATCAAATCC
    TTCCAGGCAC TGCCTTATGT TGCTTTGCTG ATAGCCATGC TCTTCTTCAT CTACGCTGTC
    ATTGGCATGC AGGTGTTTGG GAAAATTGCC ATGGTGGATC ACACTCAAAT CAACAGGAAC
    AACAACTTTC AGACGTTCCC TCAGGCTGTG CTTCTGCTCT TCAGGTGTGC TACGGGTGAA
    GCCTGGCAGG AGATCATGTT AGCATGTATG CCAGGCAAGC TGTGTGACCC GGAGTCAGAC
    TATAATCCTG GGGAAGAAAT GACCTGCGGG AGCAGTTTCG CCATCATCTA CTTCATCACC
    TTCTACATGC TCTGCGCCTT CCTGATCATT AACCTGTTTG TGGCTGTCAT CATGGACAAC
    TTCGACTATC TGACCCGTGA TTGGTCAATT CTGGGGCCAC ATCACCTTGA TGAGTTTAAG
    AGGATTTGGT CAGAATATGA CCCAGAGGCC AAGGGCAGAA TAAAACATCT TGATGTAGTG
    ACGTTGTTGC GCCGTATTCA GCCTCCTCTC GGCTTTGGGA AACTCTGTCC TCATAGAGTC
    GCCTGCAAGA GGCTGGTGGC TATGAACATG CCCCTGAACA GTGACGGCAC GGTCATGTTT
    AACGCGACAC TGTTTGCTCT CGTGAGAACC GCATTGAAAA TCAAAACAGA AGGTAACCTA
    GAACAAGCGA ATGAAGAGCT CCGAGCTGTG ATTAAGAAGA TCTGGAAGAG AACCAGCATG
    AAGCTTCTGG ATCAAGTGGT TCCCCCTGCT GGTGATGATG AGGTAACGGT GGGGAAGTTC
    TATGCCACTT TCCTGATACA GGACTACTTT AGGAAATTCA AGAAACGCAA AGAGGAAGGC
    CTGGTGGGAG TTCATCCGGC ACAGAACAAC ACGGCCATCG CACTGCAGGC TGGCCTTCGT
    ACGCTGCATG ACATTGGGCC AGAAATCCGC CGTGCGATAT CATGTGACCT GCAGGATGAT
    GAGCTAGTAG ACTTTATACC AGAGGAGGAC GAAGAAATTT ATAGGCGAAA CGGAGGACTC
    TTTGGCAACC ACATCAACCA CATAAACGGA GACCCCCGGA GGAGCTCTGG CCACCAAACA
    AACGCCACAC AGCGCCCCCT GCAGGTGCAG CCGCCCCCAC ATTACGTCCA CATGGAGCAG
    CCCGTGGGGC GTCTGGGTCG CGCCAATGCC ATGGCACAGC AAAACCATCA TCGTCACCAC
    CACCACCATC ACCATCATCA TCATCACAAC AACTCCTACA ACAAATCGCC CAAGTCCACC
    AACATCAACC TCAACAACGC CAACGTGTCC AGTTTACCCA ATGGTGGACA CAATCGCTAC
    TACGAACACG CTCCCGCTAA TGGCTACCCA GGGTCATATT ACGGAGAATA CGACAAGCCC
    AGGACCCCTC ATGGGCAAAG AAGGCGCTAC TATGAGACAT ATATTAGATC GCAGGGCAGC
    GACAGAAGAC GACCCACGAT ACGCCGCGAA GAGGAGTATG AAGAAGATCG CTACTCTGGA
    GAGTATTACA GCGGAGAGGA GTTTTATGAA GATGACAGCA TGCTATCGGG TGATAGGTAC
    CCCAACAGTG ACCAGGAGTA CGAGACGCCG CGGGGTTACC ATCATCCTGA CAGTTACTAT
    GAAGATGATG AGCAGCCCCT TTATCACGAT TCACATAGGT CACCAAAGAG AAGACTGCTT
    CCTCCAACAC CGCAAGGTAA TCGGAGACCG TCGTTCAATT TTGAGTGTCT GCGCAGACAG
    AGCAGTCAAG ACGATCTTCC TCACCAGCGA ACTGCTCTTC CGTTACATCT CATGCAACAC
    CAGGTGATGG CAGTGGCGGG TCTGGACTCT AGCAGGGCTC ACAGGCTTTC ACCAACTCGA
    TCTACCAGAT CATGGGCTTC ACCTCCTCCA ACTCCTGCAA GTAAAGACCG GACGCCGTAC
    TACACACCGC TCATCCGCGT GGACCGTCCT CTAAGAGACA GCGCCAGCAG CAGCCATTCA
    TCCATCAGGA AGAGCTCGTG GTACACAGAC GACCCTGAGT ACCAGCAGAG GAACTTCTCA
    CCTGTTCACC TGCAGGTCCC ACCCGAGTAC AGGAACCAGT ACCTGCAGAA GAGAGGCAGC
    GCTACCAGCC TGGTGGAGGC GGTCTTGATA TCAGAAGGAC TGGGTCGATA CGCCAAGGAT
    CCCAAATTTG TGGCCGCCAC AAAGCATGAG ATTGCGGACG CGTGCGAGAT GACCATTGAC
    GAAATGGAAA GCGCCGCCAG CCATCTTCTT AACGGTGGCA TTACACCAGT AGTCAACGGA
    GTCAATGTGT TTCCCATACT GGGCCACAGA GACTACGAGC TGCAGGACGT CTCGGCGAGC
    TACAGCGACG AGGAGCCCGA ACCCGAGCCC AGACCTCGTT ACGAGGAGGA CCTTGCAGAT
    GAAATGATAT GCATTACCAC TTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147862
    Clone ID Related Accession (Same CDS sequence) XM_021479582.1
    Accession Version XM_021479582.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCCGA GTATGCCGGC AAGTGAGACT GAATCCATGA ATACAGAGAA CGAGAAAGGA
    GAAGATGAGA AGGCGACATG TTGCGGCCCG ACATGTCAAA AAATATCAAA ATCAAAGTTC
    AGTCGGCGCT GGCGTCGCTG GAACCGGTTG TGTCGGAGGA ATTGTCGCTT GGCTGTAAAA
    TCTGTGCCAT TCTATTGGCT GGTAATCATC CTGGTGTTCC TGAACACTTT GACCATCTCT
    TCAGAACATT ACAACCAGCC CATGTGGCTC ACACAAGTAC AGGATGTGGC TAATAAAGTG
    CTGCTGGCCA TGTTCACGTG TGAGATGTTG GTGAAGATGT ACAGTCTGGG CCTGCAGGCT
    TATTTTGTGT CTCTTTTTAA TCGCTTTGAC TGCTTTGTGG TGTGTGGAGG CATCACTGAA
    ACCATCCTTG TGGAGTTTGA GATTATGTCA CCCCTCGGCA TCTCAGTGTT TCGATGTGTT
    CGACTTCTCA GGATCTTTAA AGTTACACGC CACTGGGCGT CTCTCAGTAA CCTGGTGGCA
    TCTCTGCTGA ACTCCATGAA GTCCATTGCT TCTCTACTGC TGCTGCTCTT CCTCTTCATC
    ATCATCTTTA GCCTCCTCGG TATGCAGGTG TTCGGCGGCA AGTTTAACTT TGACGAGACA
    CAAACCAAGA GGAGCACCTT TGACAACTTC CCTCAGGCCT TGCTCACTGT GTTTCAGATT
    CTAACTGGTG AAGACTGGAA TGCCGTCATG TATGACGGAA TTATGGCGTA CGGCGGCCCT
    TCGTCATCAG GAATGATTGT GTGCATATAC TTCATCATCC TTTTCATCTG TGGAAACTAC
    ATTCTCCTGA ATGTGTTCTT GGCTATCGCT GTGGACAATC TTGCTGATGC AGAGTCTCTG
    AACACGGACG ATACGAAGAA ACCTGATGAA ATCGACGAAA TTGAAGATGA AGCCAAGGCT
    GGAGAGGAGG ATGAGAAGGA TAATGCCGAG GAGGATGAAG AAGAGCCAGA TGTTCCTGCA
    GGGCCTCGTC CAAAGATCTC AGAGCTTGTT AAGAAGGAGA AGATCACTCC AATCCCAGAG
    GGCAGTGCGT TCTTCATCTT CAGCAACACA AACCCAGTTC GAGTCGCCTG CCACAAGCTG
    ATAAACCACC ACATCTTCAC CAACCTCATC CTTGTGTTCA TTATGCTAAG CTCAGCGTCA
    TTGGCTGCTG AGGATCCAAT CAGAAACTTC TCTGCTAGGA ATATTGTCCT AGGCTATGCA
    GATTATGTCT TCACTAGTAT GTTTACATTT GAGATCGTGT TAAAGATGAC CACGTATGGC
    GCTTTTCTCC ATAAGGGTGC TTTCTGCAGG AACTACTTTA ATCTTCTAGA TCTGCTGGTG
    GTCGGAGTTT CGCTCGTGTC CTTCGGGATT CAATCATCGG CCATCTCAGT GGTGAAGATT
    CTGAGGGTTC TGAGAGTTTT GCGTCCACTC AGAGCCATAA ACAGAGCTAA AGGACTAAAG
    CATGTGGTCC AGTGTGTGTT TGTGGCCATC AGGACTATAG GAAACATCAT GATCGTCACC
    ACTCTGCTGC AGTTTATGTT CGCCTGCATT GGTGTCCAGC TCTTCAAGGG AAAGTTTTAC
    CGTTGTAACG ATGAGGCCAA GTCCAGCCCA GAGGAGTGCA AGGGAACGTA CATCATGTAT
    AAGGAAGGAG ACGTGAACCA GCCAATCATT CAGAAGCGCC ACTGGCACAA CAGTGATTTC
    AACTTTGACA ATGTGCTCAT GGCCATGATG GCTCTGTTCA CCGTGTCCAC GTTTGAAGGC
    TGGCCAGCGT TGCTGTACAA GGCCATCGAC TCCAACCGGG AGAACATGGG TCCCATCTAC
    AACTACCGAG TGGAGATTTC CATCTTCTTC ATCATCTACA TCATCATCAT CGCTTTCTTT
    ATGATGAACA TCTTTGTGGG TTTCGTCATC GTGACCTTTC AGGAACAGGG AGAGAAGGAG
    TACAAGAACT GTGAGCTGGA CAAGAACCAG CGTCAGTGTG TGGAGTATGC ATTGAAAGCA
    CGTCCTCTCA GGAGGTACAT CCCAAAGAAC CCATATCAGT ATAAGTTCTG GTATGTGGTG
    AACTCCACCG GCTTCGAGTA CATCATGTTT GTCCTGATTT TACTCAACAC CATCTGTCTC
    GCCGTGCAGC ACTATGGTCA GTCTGAGCTC TTCAATTATG TTATGGACAT TCTGAATATG
    GTCTTCACTG CCGTCTTCAC TGTGGAGATG GTGCTCAAGC TGATCGCCTT CAAGCCTCGG
    CATTATTTCA CTGACGCATG GAACACATTT GACGCCTTAA TTGTTGTCGG TAGCGTCGTA
    GATATTGCTA TCACTGAAGT CAACCCCACA GAGGCACCTC AGGTGGATGA GTCGGGGAAC
    ACTGAGGACA GCGCTCGCAT TTCCATCACT TTCTTCCGTC TTTTTCGAGT GATGCGATTG
    GTCAAGCTGC TGAGCAGAGG GGAGGGAATT CGAACCCTAC TCTGGACCTT TATCAAATCC
    TTCCAGGCAC TGCCTTATGT TGCTTTGCTG ATAGCCATGC TCTTCTTCAT CTACGCTGTC
    ATTGGCATGC AGGTGTTTGG GAAAATTGCC ATGGTGGATC ACACTCAAAT CAACAGGAAC
    AACAACTTTC AGACGTTCCC TCAGGCTGTG CTTCTGCTCT TCAGGTGTGC TACGGGTGAA
    GCCTGGCAGG AGATCATGTT AGCATGTATG CCAGGCAAGC TGTGTGACCC GGAGTCAGAC
    TATAATCCTG GGGAAGAAAT GACCTGCGGG AGCAGTTTCG CCATCATCTA CTTCATCACC
    TTCTACATGC TCTGCGCCTT CCTGATCATT AACCTGTTTG TGGCTGTCAT CATGGACAAC
    TTCGACTATC TGACCCGTGA TTGGTCAATT CTGGGGCCAC ATCACCTTGA TGAGTTTAAG
    AGGATTTGGT CAGAATATGA CCCAGAGGCC AAGGGCAGAA TAAAACATCT TGATGTAGTG
    ACGTTGTTGC GCCGTATTCA GCCTCCTCTC GGCTTTGGGA AACTCTGTCC TCATAGAGTC
    GCCTGCAAGA GGCTGGTGGC TATGAACATG CCCCTGAACA GTGACGGCAC GGTCATGTTT
    AACGCGACAC TGTTTGCTCT CGTGAGAACC GCATTGAAAA TCAAAACAGA AGGTAACCTA
    GAACAAGCGA ATGAAGAGCT CCGAGCTGTG ATTAAGAAGA TCTGGAAGAG AACCAGCATG
    AAGCTTCTGG ATCAAGTGGT TCCCCCTGCT GGTGATGATG AGGTAACGGT GGGGAAGTTC
    TATGCCACTT TCCTGATACA GGACTACTTT AGGAAATTCA AGAAACGCAA AGAGGAAGGC
    CTGGTGGGAG TTCATCCGGC ACAGAACAAC ACGGCCATCG CACTGCAGGC TGGCCTTCGT
    ACGCTGCATG ACATTGGGCC AGAAATCCGC CGTGCGATAT CATGTGACCT GCAGGATGAT
    GAGCTAGTAG ACTTTATACC AGAGGAGGAC GAAGAAATTT ATAGGCGAAA CGGAGGACTC
    TTTGGCAACC ACATCAACCA CATAAACGGA GACCCCCGGA GGAGCTCTGG CCACCAAACA
    AACGCCACAC AGCGCCCCCT GCAGGTGCAG CCGCCCCCAC ATTACGTCCA CATGGAGCAG
    CCCGTGGGGC GTCTGGGTCG CGCCAATGCC ATGGCACAGC AAAACCATCA TCGTCACCAC
    CACCACCATC ACCATCATCA TCATCACAAC AACTCCTACA ACAAATCGCC CAAGTCCACC
    AACATCAACC TCAACAACGC CAACGTGTCC AGTTTACCCA ATGGTGGACA CAATCGCTAC
    TACGAACACG CTCCCGCTAA TGGCTACCCA GGGTCATATT ACGGAGAATA CGACAAGCCC
    AGGACCCCTC ATGGGCAAAG AAGGCGCTAC TATGAGACAT ATATTAGATC GCAGGGCAGC
    GACAGAAGAC GACCCACGAT ACGCCGCGAA GAGGAGTATG AAGAAGATCG CTACTCTGGA
    GAGTATTACA GCGGAGAGGA GTTTTATGAA GATGACAGCA TGCTATCGGG TGATAGGTAC
    CCCAACAGTG ACCAGGAGTA CGAGACGCCG CGGGGTTACC ATCATCCTGA CAGTTACTAT
    GAAGATGATG AGCAGCCCCT TTATCACGAT TCACATAGGT CACCAAAGAG AAGACTGCTT
    CCTCCAACAC CGCAAGGTAA TCGGAGACCG TCGTTCAATT TTGAGTGTCT GCGCAGACAG
    AGCAGTCAAG ACGATCTTCC TCACCAGCGA ACTGCTCTTC CGTTACATCT CATGCAACAC
    CAGGTGATGG CAGTGGCGGG TCTGGACTCT AGCAGGGCTC ACAGGCTTTC ACCAACTCGA
    TCTACCAGAT CATGGGCTTC ACCTCCTCCA ACTCCTGCAA GTAAAGACCG GACGCCGTAC
    TACACACCGC TCATCCGCGT GGACCGTCCT CTAAGAGACA GCGCCAGCAG CAGCCATTCA
    TCCATCAGGA AGAGCTCGTG GTACACAGAC GACCCTGAGT ACCAGCAGAG GAACTTCTCA
    CCTGTTCACC TGCAGGTCCC ACCCGAGTAC AGGAACCAGT ACCTGCAGAA GAGAGGCAGC
    GCTACCAGCC TGGTGGAGGC GGTCTTGATA TCAGAAGGAC TGGGTCGATA CGCCAAGGAT
    CCCAAATTTG TGGCCGCCAC AAAGCATGAG ATTGCGGACG CGTGCGAGAT GACCATTGAC
    GAAATGGAAA GCGCCGCCAG CCATCTTCTT AACGGTGGCA TTACACCAGT AGTCAACGGA
    GTCAATGTGT TTCCCATACT GGGCCACAGA GACTACGAGC TGCAGGACGT CTCGGCGAGC
    TACAGCGACG AGGAGCCCGA ACCCGAGCCC AGACCTCGTT ACGAGGAGGA CCTTGCAGAT
    GAAATGATAT GCATTACCAC TTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335257.1
    CDS105..6311
    Translation

    Target ORF information:

    RefSeq Version XM_021479582.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479582.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCCGA GTATGCCGGC AAGTGAGACT GAATCCATGA ATACAGAGAA CGAGAAAGGA
    GAAGATGAGA AGGCGACATG TTGCGGCCCG ACATGTCAAA AAATATCAAA ATCAAAGTTC
    AGTCGGCGCT GGCGTCGCTG GAACCGGTTG TGTCGGAGGA ATTGTCGCTT GGCTGTAAAA
    TCTGTGCCAT TCTATTGGCT GGTAATCATC CTGGTGTTCC TGAACACTTT GACCATCTCT
    TCAGAACATT ACAACCAGCC CATGTGGCTC ACACAAGTAC AGGATGTGGC TAATAAAGTG
    CTGCTGGCCA TGTTCACGTG TGAGATGTTG GTGAAGATGT ACAGTCTGGG CCTGCAGGCT
    TATTTTGTGT CTCTTTTTAA TCGCTTTGAC TGCTTTGTGG TGTGTGGAGG CATCACTGAA
    ACCATCCTTG TGGAGTTTGA GATTATGTCA CCCCTCGGCA TCTCAGTGTT TCGATGTGTT
    CGACTTCTCA GGATCTTTAA AGTTACACGC CACTGGGCGT CTCTCAGTAA CCTGGTGGCA
    TCTCTGCTGA ACTCCATGAA GTCCATTGCT TCTCTACTGC TGCTGCTCTT CCTCTTCATC
    ATCATCTTTA GCCTCCTCGG TATGCAGGTG TTCGGCGGCA AGTTTAACTT TGACGAGACA
    CAAACCAAGA GGAGCACCTT TGACAACTTC CCTCAGGCCT TGCTCACTGT GTTTCAGATT
    CTAACTGGTG AAGACTGGAA TGCCGTCATG TATGACGGAA TTATGGCGTA CGGCGGCCCT
    TCGTCATCAG GAATGATTGT GTGCATATAC TTCATCATCC TTTTCATCTG TGGAAACTAC
    ATTCTCCTGA ATGTGTTCTT GGCTATCGCT GTGGACAATC TTGCTGATGC AGAGTCTCTG
    AACACGGACG ATACGAAGAA ACCTGATGAA ATCGACGAAA TTGAAGATGA AGCCAAGGCT
    GGAGAGGAGG ATGAGAAGGA TAATGCCGAG GAGGATGAAG AAGAGCCAGA TGTTCCTGCA
    GGGCCTCGTC CAAAGATCTC AGAGCTTGTT AAGAAGGAGA AGATCACTCC AATCCCAGAG
    GGCAGTGCGT TCTTCATCTT CAGCAACACA AACCCAGTTC GAGTCGCCTG CCACAAGCTG
    ATAAACCACC ACATCTTCAC CAACCTCATC CTTGTGTTCA TTATGCTAAG CTCAGCGTCA
    TTGGCTGCTG AGGATCCAAT CAGAAACTTC TCTGCTAGGA ATATTGTCCT AGGCTATGCA
    GATTATGTCT TCACTAGTAT GTTTACATTT GAGATCGTGT TAAAGATGAC CACGTATGGC
    GCTTTTCTCC ATAAGGGTGC TTTCTGCAGG AACTACTTTA ATCTTCTAGA TCTGCTGGTG
    GTCGGAGTTT CGCTCGTGTC CTTCGGGATT CAATCATCGG CCATCTCAGT GGTGAAGATT
    CTGAGGGTTC TGAGAGTTTT GCGTCCACTC AGAGCCATAA ACAGAGCTAA AGGACTAAAG
    CATGTGGTCC AGTGTGTGTT TGTGGCCATC AGGACTATAG GAAACATCAT GATCGTCACC
    ACTCTGCTGC AGTTTATGTT CGCCTGCATT GGTGTCCAGC TCTTCAAGGG AAAGTTTTAC
    CGTTGTAACG ATGAGGCCAA GTCCAGCCCA GAGGAGTGCA AGGGAACGTA CATCATGTAT
    AAGGAAGGAG ACGTGAACCA GCCAATCATT CAGAAGCGCC ACTGGCACAA CAGTGATTTC
    AACTTTGACA ATGTGCTCAT GGCCATGATG GCTCTGTTCA CCGTGTCCAC GTTTGAAGGC
    TGGCCAGCGT TGCTGTACAA GGCCATCGAC TCCAACCGGG AGAACATGGG TCCCATCTAC
    AACTACCGAG TGGAGATTTC CATCTTCTTC ATCATCTACA TCATCATCAT CGCTTTCTTT
    ATGATGAACA TCTTTGTGGG TTTCGTCATC GTGACCTTTC AGGAACAGGG AGAGAAGGAG
    TACAAGAACT GTGAGCTGGA CAAGAACCAG CGTCAGTGTG TGGAGTATGC ATTGAAAGCA
    CGTCCTCTCA GGAGGTACAT CCCAAAGAAC CCATATCAGT ATAAGTTCTG GTATGTGGTG
    AACTCCACCG GCTTCGAGTA CATCATGTTT GTCCTGATTT TACTCAACAC CATCTGTCTC
    GCCGTGCAGC ACTATGGTCA GTCTGAGCTC TTCAATTATG TTATGGACAT TCTGAATATG
    GTCTTCACTG CCGTCTTCAC TGTGGAGATG GTGCTCAAGC TGATCGCCTT CAAGCCTCGG
    CATTATTTCA CTGACGCATG GAACACATTT GACGCCTTAA TTGTTGTCGG TAGCGTCGTA
    GATATTGCTA TCACTGAAGT CAACCCCACA GAGGCACCTC AGGTGGATGA GTCGGGGAAC
    ACTGAGGACA GCGCTCGCAT TTCCATCACT TTCTTCCGTC TTTTTCGAGT GATGCGATTG
    GTCAAGCTGC TGAGCAGAGG GGAGGGAATT CGAACCCTAC TCTGGACCTT TATCAAATCC
    TTCCAGGCAC TGCCTTATGT TGCTTTGCTG ATAGCCATGC TCTTCTTCAT CTACGCTGTC
    ATTGGCATGC AGGTGTTTGG GAAAATTGCC ATGGTGGATC ACACTCAAAT CAACAGGAAC
    AACAACTTTC AGACGTTCCC TCAGGCTGTG CTTCTGCTCT TCAGGTGTGC TACGGGTGAA
    GCCTGGCAGG AGATCATGTT AGCATGTATG CCAGGCAAGC TGTGTGACCC GGAGTCAGAC
    TATAATCCTG GGGAAGAAAT GACCTGCGGG AGCAGTTTCG CCATCATCTA CTTCATCACC
    TTCTACATGC TCTGCGCCTT CCTGATCATT AACCTGTTTG TGGCTGTCAT CATGGACAAC
    TTCGACTATC TGACCCGTGA TTGGTCAATT CTGGGGCCAC ATCACCTTGA TGAGTTTAAG
    AGGATTTGGT CAGAATATGA CCCAGAGGCC AAGGGCAGAA TAAAACATCT TGATGTAGTG
    ACGTTGTTGC GCCGTATTCA GCCTCCTCTC GGCTTTGGGA AACTCTGTCC TCATAGAGTC
    GCCTGCAAGA GGCTGGTGGC TATGAACATG CCCCTGAACA GTGACGGCAC GGTCATGTTT
    AACGCGACAC TGTTTGCTCT CGTGAGAACC GCATTGAAAA TCAAAACAGA AGGTAACCTA
    GAACAAGCGA ATGAAGAGCT CCGAGCTGTG ATTAAGAAGA TCTGGAAGAG AACCAGCATG
    AAGCTTCTGG ATCAAGTGGT TCCCCCTGCT GGTGATGATG AGGTAACGGT GGGGAAGTTC
    TATGCCACTT TCCTGATACA GGACTACTTT AGGAAATTCA AGAAACGCAA AGAGGAAGGC
    CTGGTGGGAG TTCATCCGGC ACAGAACAAC ACGGCCATCG CACTGCAGGC TGGCCTTCGT
    ACGCTGCATG ACATTGGGCC AGAAATCCGC CGTGCGATAT CATGTGACCT GCAGGATGAT
    GAGCTAGTAG ACTTTATACC AGAGGAGGAC GAAGAAATTT ATAGGCGAAA CGGAGGACTC
    TTTGGCAACC ACATCAACCA CATAAACGGA GACCCCCGGA GGAGCTCTGG CCACCAAACA
    AACGCCACAC AGCGCCCCCT GCAGGTGCAG CCGCCCCCAC ATTACGTCCA CATGGAGCAG
    CCCGTGGGGC GTCTGGGTCG CGCCAATGCC ATGGCACAGC AAAACCATCA TCGTCACCAC
    CACCACCATC ACCATCATCA TCATCACAAC AACTCCTACA ACAAATCGCC CAAGTCCACC
    AACATCAACC TCAACAACGC CAACGTGTCC AGTTTACCCA ATGGTGGACA CAATCGCTAC
    TACGAACACG CTCCCGCTAA TGGCTACCCA GGGTCATATT ACGGAGAATA CGACAAGCCC
    AGGACCCCTC ATGGGCAAAG AAGGCGCTAC TATGAGACAT ATATTAGATC GCAGGGCAGC
    GACAGAAGAC GACCCACGAT ACGCCGCGAA GAGGAGTATG AAGAAGATCG CTACTCTGGA
    GAGTATTACA GCGGAGAGGA GTTTTATGAA GATGACAGCA TGCTATCGGG TGATAGGTAC
    CCCAACAGTG ACCAGGAGTA CGAGACGCCG CGGGGTTACC ATCATCCTGA CAGTTACTAT
    GAAGATGATG AGCAGCCCCT TTATCACGAT TCACATAGGT CACCAAAGAG AAGACTGCTT
    CCTCCAACAC CGCAAGGTAA TCGGAGACCG TCGTTCAATT TTGAGTGTCT GCGCAGACAG
    AGCAGTCAAG ACGATCTTCC TCACCAGCGA ACTGCTCTTC CGTTACATCT CATGCAACAC
    CAGGTGATGG CAGTGGCGGG TCTGGACTCT AGCAGGGCTC ACAGGCTTTC ACCAACTCGA
    TCTACCAGAT CATGGGCTTC ACCTCCTCCA ACTCCTGCAA GTAAAGACCG GACGCCGTAC
    TACACACCGC TCATCCGCGT GGACCGTCCT CTAAGAGACA GCGCCAGCAG CAGCCATTCA
    TCCATCAGGA AGAGCTCGTG GTACACAGAC GACCCTGAGT ACCAGCAGAG GAACTTCTCA
    CCTGTTCACC TGCAGGTCCC ACCCGAGTAC AGGAACCAGT ACCTGCAGAA GAGAGGCAGC
    GCTACCAGCC TGGTGGAGGC GGTCTTGATA TCAGAAGGAC TGGGTCGATA CGCCAAGGAT
    CCCAAATTTG TGGCCGCCAC AAAGCATGAG ATTGCGGACG CGTGCGAGAT GACCATTGAC
    GAAATGGAAA GCGCCGCCAG CCATCTTCTT AACGGTGGCA TTACACCAGT AGTCAACGGA
    GTCAATGTGT TTCCCATACT GGGCCACAGA GACTACGAGC TGCAGGACGT CTCGGCGAGC
    TACAGCGACG AGGAGCCCGA ACCCGAGCCC AGACCTCGTT ACGAGGAGGA CCTTGCAGAT
    GAAATGATAT GCATTACCAC TTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147863
    Clone ID Related Accession (Same CDS sequence) XM_021479583.1
    Accession Version XM_021479583.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCGGG TTACACTTGC CAGCCTCATG GAGAAGAAGA AGAAGGGGTT TGGCTGGTTC
    AGCCAGTCCT CTGACACTCA TGCGAGTATG CCGGCAAGTG AGACTGAATC CATGAATACA
    GAGAACGAGA AAGGAGAAGA TGAGAAGGCG ACATGTTGCG GCCCGACATG TCAAAAAATA
    TCAAAATCAA AGTTCAGTCG GCGCTGGCGT CGCTGGAACC GGTTGTGTCG GAGGAATTGT
    CGCTTGGCTG TAAAATCTGT GCCATTCTAT TGGCTGGTAA TCATCCTGGT GTTCCTGAAC
    ACTTTGACCA TCTCTTCAGA ACATTACAAC CAGCCCATGT GGCTCACACA AGTACAGGAT
    GTGGCTAATA AAGTGCTGCT GGCCATGTTC ACGTGTGAGA TGTTGGTGAA GATGTACAGT
    CTGGGCCTGC AGGCTTATTT TGTGTCTCTT TTTAATCGCT TTGACTGCTT TGTGGTGTGT
    GGAGGCATCA CTGAAACCAT CCTTGTGGAG TTTGAGATTA TGTCACCCCT CGGCATCTCA
    GTGTTTCGAT GTGTTCGACT TCTCAGGATC TTTAAAGTTA CACGCCACTG GGCGTCTCTC
    AGTAACCTGG TGGCATCTCT GCTGAACTCC ATGAAGTCCA TTGCTTCTCT ACTGCTGCTG
    CTCTTCCTCT TCATCATCAT CTTTAGCCTC CTCGGTATGC AGGTGTTCGG CGGCAAGTTT
    AACTTTGACG AGACACAAAC CAAGAGGAGC ACCTTTGACA ACTTCCCTCA GGCCTTGCTC
    ACTGTGTTTC AGATTCTAAC TGGTGAAGAC TGGAATGCCG TCATGTATGA CGGAATTATG
    GCGTACGGCG GCCCTTCGTC ATCAGGAATG ATTGTGTGCA TATACTTCAT CATCCTTTTC
    ATCTGTGGAA ACTACATTCT CCTGAATGTG TTCTTGGCTA TCGCTGTGGA CAATCTTGCT
    GATGCAGAGT CTCTGAACAC GGACGATACG AAGAAACCTG ATGAAATCGA CGAAATTGAA
    GATGAAGCCA AGGCTGGAGA GGAGGATGAG AAGGATAATG CCGAGGAGGA TGAAGAAGAG
    CCAGATGTTC CTGCAGGGCC TCGTCCAAAG ATCTCAGAGC TTGTTAAGAA GGAGAAGATC
    ACTCCAATCC CAGAGGGCAG TGCGTTCTTC ATCTTCAGCA ACACAAACCC AGTTCGAGTC
    GCCTGCCACA AGCTGATAAA CCACCACATC TTCACCAACC TCATCCTTGT GTTCATTATG
    CTAAGCTCAG CGTCATTGGC TGCTGAGGAT CCAATCAGAA ACTTCTCTGC TAGGAATATT
    ATACTTGGTT ACTTTGACTA TGCTTTCACG GCTATCTTTA CTGTAGAGAT CGTGTTAAAG
    ATGACCACGT ATGGCGCTTT TCTCCATAAG GGTGCTTTCT GCAGGAACTA CTTTAATCTT
    CTAGATCTGC TGGTGGTCGG AGTTTCGCTC GTGTCCTTCG GGATTCAATC ATCGGCCATC
    TCAGTGGTGA AGATTCTGAG GGTTCTGAGA GTTTTGCGTC CACTCAGAGC CATAAACAGA
    GCTAAAGGAC TAAAGCATGT GGTCCAGTGT GTGTTTGTGG CCATCAGGAC TATAGGAAAC
    ATCATGATCG TCACCACTCT GCTGCAGTTT ATGTTCGCCT GCATTGGTGT CCAGCTCTTC
    AAGGGAAAGT TTTACCGTTG TAACGATGAG GCCAAGTCCA GCCCAGAGGA GTGCAAGGGA
    ACGTACATCA TGTATAAGGA AGGAGACGTG AACCAGCCAA TCATTCAGAA GCGCCACTGG
    CACAACAGTG ATTTCAACTT TGACAATGTG CTCATGGCCA TGATGGCTCT GTTCACCGTG
    TCCACGTTTG AAGGCTGGCC AGCGTTGCTG TACAAGGCCA TCGACTCCAA CCGGGAGAAC
    ATGGGTCCCA TCTACAACTA CCGAGTGGAG ATTTCCATCT TCTTCATCAT CTACATCATC
    ATCATCGCTT TCTTTATGAT GAACATCTTT GTGGGTTTCG TCATCGTGAC CTTTCAGGAA
    CAGGGAGAGA AGGAGTACAA GAACTGTGAG CTGGACAAGA ACCAGCGTCA GTGTGTGGAG
    TATGCATTGA AAGCACGTCC TCTCAGGAGG TACATCCCAA AGAACCCATA TCAGTATAAG
    TTCTGGTATG TGGTGAACTC CACCGGCTTC GAGTACATCA TGTTTGTCCT GATTTTACTC
    AACACCATCT GTCTCGCCGT GCAGCACTAT GGTCAGTCTG AGCTCTTCAA TTATGTTATG
    GACATTCTGA ATATGGTCTT CACTGCCGTC TTCACTGTGG AGATGGTGCT CAAGCTGATC
    GCCTTCAAGC CTCGGCATTA TTTCACTGAC GCATGGAACA CATTTGACGC CTTAATTGTT
    GTCGGTAGCG TCGTAGATAT TGCTATCACT GAAGTCAACC CCACAGAGGC ACCTCAGGTG
    GATGAGTCGG GGAACACTGA GGACAGCGCT CGCATTTCCA TCACTTTCTT CCGTCTTTTT
    CGAGTGATGC GATTGGTCAA GCTGCTGAGC AGAGGGGAGG GAATTCGAAC CCTACTCTGG
    ACCTTTATCA AATCCTTCCA GGCACTGCCT TATGTTGCTT TGCTGATAGC CATGCTCTTC
    TTCATCTACG CTGTCATTGG CATGCAGGTG TTTGGGAAAA TTGCCATGGT GGATCACACT
    CAAATCAACA GGAACAACAA CTTTCAGACG TTCCCTCAGG CTGTGCTTCT GCTCTTCAGG
    TGTGCTACGG GTGAAGCCTG GCAGGAGATC ATGTTAGCAT GTATGCCAGG CAAGCTGTGT
    GACCCGGAGT CAGACTATAA TCCTGGGGAA GAAATGACCT GCGGGAGCAG TTTCGCCATC
    ATCTACTTCA TCACCTTCTA CATGCTCTGC GCCTTCCTGA TCATTAACCT GTTTGTGGCT
    GTCATCATGG ACAACTTCGA CTATCTGACC CGTGATTGGT CAATTCTGGG GCCACATCAC
    CTTGATGAGT TTAAGAGGAT TTGGTCAGAA TATGACCCAG AGGCCAAGGG CAGAATAAAA
    CATCTTGATG TAGTGACGTT GTTGCGCCGT ATTCAGCCTC CTCTCGGCTT TGGGAAACTC
    TGTCCTCATA GAGTCGCCTG CAAGAGGCTG GTGGCTATGA ACATGCCCCT GAACAGTGAC
    GGCACGGTCA TGTTTAACGC GACACTGTTT GCTCTCGTGA GAACCGCATT GAAAATCAAA
    ACAGAAGGTA ACCTAGAACA AGCGAATGAA GAGCTCCGAG CTGTGATTAA GAAGATCTGG
    AAGAGAACCA GCATGAAGCT TCTGGATCAA GTGGTTCCCC CTGCTGGTGA TGATGAGGTA
    ACGGTGGGGA AGTTCTATGC CACTTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA
    CGCAAAGAGG AAGGCCTGGT GGGAGTTCAT CCGGCACAGA ACAACACGGC CATCGCACTG
    CAGCGAAACG GAGGACTCTT TGGCAACCAC ATCAACCACA TAAACGGAGA CCCCCGGAGG
    AGCTCTGGCC ACCAAACAAA CGCCACACAG CGCCCCCTGC AGGTGCAGCC GCCCCCACAT
    TACGTCCACA TGGAGCAGCC CGTGGGGCGT CTGGGTCGCG CCAATGCCAT GGCACAGCAA
    AACCATCATC GTCACCACCA CCACCATCAC CATCATCATC ATCACAACAA CTCCTACAAC
    AAATCGCCCA AGTCCACCAA CATCAACCTC AACAACGCCA ACGTGTCCAG TTTACCCAAT
    GGTGGACACA ATCGCTACTA CGAACACGCT CCCGCTAATG GCTACCCAGG GTCATATTAC
    GGAGAATACG ACAAGCCCAG GACCCCTCAT GGGCAAAGAA GGCGCTACTA TGAGACATAT
    ATTAGATCGC AGGGCAGCGA CAGAAGACGA CCCACGATAC GCCGCGAAGA GGAGTATGAA
    GAAGATCGCT ACTCTGGAGA GTATTACAGC GGAGAGGAGT TTTATGAAGA TGACAGCATG
    CTATCGGGTG ATAGGTACCC CAACAGTGAC CAGGAGTACG AGACGCCGCG GGGTTACCAT
    CATCCTGACA GTTACTATGA AGATGATGAG CAGCCCCTTT ATCACGATTC ACATAGGTCA
    CCAAAGAGAA GACTGCTTCC TCCAACACCG CAAGGTAATC GGAGACCGTC GTTCAATTTT
    GAGTGTCTGC GCAGACAGAG CAGTCAAGAC GATCTTCCTC ACCAGCGAAC TGCTCTTCCG
    TTACATCTCA TGCAACACCA GGTGATGGCA GTGGCGGGTC TGGACTCTAG CAGGGCTCAC
    AGGCTTTCAC CAACTCGATC TACCAGATCA TGGGCTTCAC CTCCTCCAAC TCCTGCAAGT
    AAAGACCGGA CGCCGTACTA CACACCGCTC ATCCGCGTGG ACCGTCCTCT AAGAGACAGC
    GCCAGCAGCA GCCATTCATC CATCAGGAAG AGCTCGTGGT ACACAGACGA CCCTGAGTAC
    CAGCAGAGGA ACTTCTCACC TGTTCACCTG CAGGTCCCAC CCGAGTACAG GAACCAGTAC
    CTGCAGAAGA GAGGCAGCGC TACCAGCCTG GTGGAGGCGG TCTTGATATC AGAAGGACTG
    GGTCGATACG CCAAGGATCC CAAATTTGTG GCCGCCACAA AGCATGAGAT TGCGGACGCG
    TGCGAGATGA CCATTGACGA AATGGAAAGC GCCGCCAGCC ATCTTCTTAA CGGTGGCATT
    ACACCAGTAG TCAACGGAGT CAATGTGTTT CCCATACTGG GCCACAGAGA CTACGAGCTG
    CAGGACGTCT CGGCGAGCTA CAGCGACGAG GAGCCCGAAC CCGAGCCCAG ACCTCGTTAC
    GAGGAGGACC TTGCAGATGA AATGATATGC ATTACCACTT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335258.1
    CDS411..6575
    Translation

    Target ORF information:

    RefSeq Version XM_021479583.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479583.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCGGG TTACACTTGC CAGCCTCATG GAGAAGAAGA AGAAGGGGTT TGGCTGGTTC
    AGCCAGTCCT CTGACACTCA TGCGAGTATG CCGGCAAGTG AGACTGAATC CATGAATACA
    GAGAACGAGA AAGGAGAAGA TGAGAAGGCG ACATGTTGCG GCCCGACATG TCAAAAAATA
    TCAAAATCAA AGTTCAGTCG GCGCTGGCGT CGCTGGAACC GGTTGTGTCG GAGGAATTGT
    CGCTTGGCTG TAAAATCTGT GCCATTCTAT TGGCTGGTAA TCATCCTGGT GTTCCTGAAC
    ACTTTGACCA TCTCTTCAGA ACATTACAAC CAGCCCATGT GGCTCACACA AGTACAGGAT
    GTGGCTAATA AAGTGCTGCT GGCCATGTTC ACGTGTGAGA TGTTGGTGAA GATGTACAGT
    CTGGGCCTGC AGGCTTATTT TGTGTCTCTT TTTAATCGCT TTGACTGCTT TGTGGTGTGT
    GGAGGCATCA CTGAAACCAT CCTTGTGGAG TTTGAGATTA TGTCACCCCT CGGCATCTCA
    GTGTTTCGAT GTGTTCGACT TCTCAGGATC TTTAAAGTTA CACGCCACTG GGCGTCTCTC
    AGTAACCTGG TGGCATCTCT GCTGAACTCC ATGAAGTCCA TTGCTTCTCT ACTGCTGCTG
    CTCTTCCTCT TCATCATCAT CTTTAGCCTC CTCGGTATGC AGGTGTTCGG CGGCAAGTTT
    AACTTTGACG AGACACAAAC CAAGAGGAGC ACCTTTGACA ACTTCCCTCA GGCCTTGCTC
    ACTGTGTTTC AGATTCTAAC TGGTGAAGAC TGGAATGCCG TCATGTATGA CGGAATTATG
    GCGTACGGCG GCCCTTCGTC ATCAGGAATG ATTGTGTGCA TATACTTCAT CATCCTTTTC
    ATCTGTGGAA ACTACATTCT CCTGAATGTG TTCTTGGCTA TCGCTGTGGA CAATCTTGCT
    GATGCAGAGT CTCTGAACAC GGACGATACG AAGAAACCTG ATGAAATCGA CGAAATTGAA
    GATGAAGCCA AGGCTGGAGA GGAGGATGAG AAGGATAATG CCGAGGAGGA TGAAGAAGAG
    CCAGATGTTC CTGCAGGGCC TCGTCCAAAG ATCTCAGAGC TTGTTAAGAA GGAGAAGATC
    ACTCCAATCC CAGAGGGCAG TGCGTTCTTC ATCTTCAGCA ACACAAACCC AGTTCGAGTC
    GCCTGCCACA AGCTGATAAA CCACCACATC TTCACCAACC TCATCCTTGT GTTCATTATG
    CTAAGCTCAG CGTCATTGGC TGCTGAGGAT CCAATCAGAA ACTTCTCTGC TAGGAATATT
    ATACTTGGTT ACTTTGACTA TGCTTTCACG GCTATCTTTA CTGTAGAGAT CGTGTTAAAG
    ATGACCACGT ATGGCGCTTT TCTCCATAAG GGTGCTTTCT GCAGGAACTA CTTTAATCTT
    CTAGATCTGC TGGTGGTCGG AGTTTCGCTC GTGTCCTTCG GGATTCAATC ATCGGCCATC
    TCAGTGGTGA AGATTCTGAG GGTTCTGAGA GTTTTGCGTC CACTCAGAGC CATAAACAGA
    GCTAAAGGAC TAAAGCATGT GGTCCAGTGT GTGTTTGTGG CCATCAGGAC TATAGGAAAC
    ATCATGATCG TCACCACTCT GCTGCAGTTT ATGTTCGCCT GCATTGGTGT CCAGCTCTTC
    AAGGGAAAGT TTTACCGTTG TAACGATGAG GCCAAGTCCA GCCCAGAGGA GTGCAAGGGA
    ACGTACATCA TGTATAAGGA AGGAGACGTG AACCAGCCAA TCATTCAGAA GCGCCACTGG
    CACAACAGTG ATTTCAACTT TGACAATGTG CTCATGGCCA TGATGGCTCT GTTCACCGTG
    TCCACGTTTG AAGGCTGGCC AGCGTTGCTG TACAAGGCCA TCGACTCCAA CCGGGAGAAC
    ATGGGTCCCA TCTACAACTA CCGAGTGGAG ATTTCCATCT TCTTCATCAT CTACATCATC
    ATCATCGCTT TCTTTATGAT GAACATCTTT GTGGGTTTCG TCATCGTGAC CTTTCAGGAA
    CAGGGAGAGA AGGAGTACAA GAACTGTGAG CTGGACAAGA ACCAGCGTCA GTGTGTGGAG
    TATGCATTGA AAGCACGTCC TCTCAGGAGG TACATCCCAA AGAACCCATA TCAGTATAAG
    TTCTGGTATG TGGTGAACTC CACCGGCTTC GAGTACATCA TGTTTGTCCT GATTTTACTC
    AACACCATCT GTCTCGCCGT GCAGCACTAT GGTCAGTCTG AGCTCTTCAA TTATGTTATG
    GACATTCTGA ATATGGTCTT CACTGCCGTC TTCACTGTGG AGATGGTGCT CAAGCTGATC
    GCCTTCAAGC CTCGGCATTA TTTCACTGAC GCATGGAACA CATTTGACGC CTTAATTGTT
    GTCGGTAGCG TCGTAGATAT TGCTATCACT GAAGTCAACC CCACAGAGGC ACCTCAGGTG
    GATGAGTCGG GGAACACTGA GGACAGCGCT CGCATTTCCA TCACTTTCTT CCGTCTTTTT
    CGAGTGATGC GATTGGTCAA GCTGCTGAGC AGAGGGGAGG GAATTCGAAC CCTACTCTGG
    ACCTTTATCA AATCCTTCCA GGCACTGCCT TATGTTGCTT TGCTGATAGC CATGCTCTTC
    TTCATCTACG CTGTCATTGG CATGCAGGTG TTTGGGAAAA TTGCCATGGT GGATCACACT
    CAAATCAACA GGAACAACAA CTTTCAGACG TTCCCTCAGG CTGTGCTTCT GCTCTTCAGG
    TGTGCTACGG GTGAAGCCTG GCAGGAGATC ATGTTAGCAT GTATGCCAGG CAAGCTGTGT
    GACCCGGAGT CAGACTATAA TCCTGGGGAA GAAATGACCT GCGGGAGCAG TTTCGCCATC
    ATCTACTTCA TCACCTTCTA CATGCTCTGC GCCTTCCTGA TCATTAACCT GTTTGTGGCT
    GTCATCATGG ACAACTTCGA CTATCTGACC CGTGATTGGT CAATTCTGGG GCCACATCAC
    CTTGATGAGT TTAAGAGGAT TTGGTCAGAA TATGACCCAG AGGCCAAGGG CAGAATAAAA
    CATCTTGATG TAGTGACGTT GTTGCGCCGT ATTCAGCCTC CTCTCGGCTT TGGGAAACTC
    TGTCCTCATA GAGTCGCCTG CAAGAGGCTG GTGGCTATGA ACATGCCCCT GAACAGTGAC
    GGCACGGTCA TGTTTAACGC GACACTGTTT GCTCTCGTGA GAACCGCATT GAAAATCAAA
    ACAGAAGGTA ACCTAGAACA AGCGAATGAA GAGCTCCGAG CTGTGATTAA GAAGATCTGG
    AAGAGAACCA GCATGAAGCT TCTGGATCAA GTGGTTCCCC CTGCTGGTGA TGATGAGGTA
    ACGGTGGGGA AGTTCTATGC CACTTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA
    CGCAAAGAGG AAGGCCTGGT GGGAGTTCAT CCGGCACAGA ACAACACGGC CATCGCACTG
    CAGCGAAACG GAGGACTCTT TGGCAACCAC ATCAACCACA TAAACGGAGA CCCCCGGAGG
    AGCTCTGGCC ACCAAACAAA CGCCACACAG CGCCCCCTGC AGGTGCAGCC GCCCCCACAT
    TACGTCCACA TGGAGCAGCC CGTGGGGCGT CTGGGTCGCG CCAATGCCAT GGCACAGCAA
    AACCATCATC GTCACCACCA CCACCATCAC CATCATCATC ATCACAACAA CTCCTACAAC
    AAATCGCCCA AGTCCACCAA CATCAACCTC AACAACGCCA ACGTGTCCAG TTTACCCAAT
    GGTGGACACA ATCGCTACTA CGAACACGCT CCCGCTAATG GCTACCCAGG GTCATATTAC
    GGAGAATACG ACAAGCCCAG GACCCCTCAT GGGCAAAGAA GGCGCTACTA TGAGACATAT
    ATTAGATCGC AGGGCAGCGA CAGAAGACGA CCCACGATAC GCCGCGAAGA GGAGTATGAA
    GAAGATCGCT ACTCTGGAGA GTATTACAGC GGAGAGGAGT TTTATGAAGA TGACAGCATG
    CTATCGGGTG ATAGGTACCC CAACAGTGAC CAGGAGTACG AGACGCCGCG GGGTTACCAT
    CATCCTGACA GTTACTATGA AGATGATGAG CAGCCCCTTT ATCACGATTC ACATAGGTCA
    CCAAAGAGAA GACTGCTTCC TCCAACACCG CAAGGTAATC GGAGACCGTC GTTCAATTTT
    GAGTGTCTGC GCAGACAGAG CAGTCAAGAC GATCTTCCTC ACCAGCGAAC TGCTCTTCCG
    TTACATCTCA TGCAACACCA GGTGATGGCA GTGGCGGGTC TGGACTCTAG CAGGGCTCAC
    AGGCTTTCAC CAACTCGATC TACCAGATCA TGGGCTTCAC CTCCTCCAAC TCCTGCAAGT
    AAAGACCGGA CGCCGTACTA CACACCGCTC ATCCGCGTGG ACCGTCCTCT AAGAGACAGC
    GCCAGCAGCA GCCATTCATC CATCAGGAAG AGCTCGTGGT ACACAGACGA CCCTGAGTAC
    CAGCAGAGGA ACTTCTCACC TGTTCACCTG CAGGTCCCAC CCGAGTACAG GAACCAGTAC
    CTGCAGAAGA GAGGCAGCGC TACCAGCCTG GTGGAGGCGG TCTTGATATC AGAAGGACTG
    GGTCGATACG CCAAGGATCC CAAATTTGTG GCCGCCACAA AGCATGAGAT TGCGGACGCG
    TGCGAGATGA CCATTGACGA AATGGAAAGC GCCGCCAGCC ATCTTCTTAA CGGTGGCATT
    ACACCAGTAG TCAACGGAGT CAATGTGTTT CCCATACTGG GCCACAGAGA CTACGAGCTG
    CAGGACGTCT CGGCGAGCTA CAGCGACGAG GAGCCCGAAC CCGAGCCCAG ACCTCGTTAC
    GAGGAGGACC TTGCAGATGA AATGATATGC ATTACCACTT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147864
    Clone ID Related Accession (Same CDS sequence) XM_021479584.1
    Accession Version XM_021479584.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCCGA GTATGCCGGC AAGTGAGACT GAATCCATGA ATACAGAGAA CGAGAAAGGA
    GAAGATGAGA AGGCGACATG TTGCGGCCCG ACATGTCAAA AAATATCAAA ATCAAAGTTC
    AGTCGGCGCT GGCGTCGCTG GAACCGGTTG TGTCGGAGGA ATTGTCGCTT GGCTGTAAAA
    TCTGTGCCAT TCTATTGGCT GGTAATCATC CTGGTGTTCC TGAACACTTT GACCATCTCT
    TCAGAACATT ACAACCAGCC CATGTGGCTC ACACAAGTAC AGGATGTGGC TAATAAAGTG
    CTGCTGGCCA TGTTCACGTG TGAGATGTTG GTGAAGATGT ACAGTCTGGG CCTGCAGGCT
    TATTTTGTGT CTCTTTTTAA TCGCTTTGAC TGCTTTGTGG TGTGTGGAGG CATCACTGAA
    ACCATCCTTG TGGAGTTTGA GATTATGTCA CCCCTCGGCA TCTCAGTGTT TCGATGTGTT
    CGACTTCTCA GGATCTTTAA AGTTACACGC CACTGGGCGT CTCTCAGTAA CCTGGTGGCA
    TCTCTGCTGA ACTCCATGAA GTCCATTGCT TCTCTACTGC TGCTGCTCTT CCTCTTCATC
    ATCATCTTTA GCCTCCTCGG TATGCAGGTG TTCGGCGGCA AGTTTAACTT TGACGAGACA
    CAAACCAAGA GGAGCACCTT TGACAACTTC CCTCAGGCCT TGCTCACTGT GTTTCAGATT
    CTAACTGGTG AAGACTGGAA TGCCGTCATG TATGACGGAA TTATGGCGTA CGGCGGCCCT
    TCGTCATCAG GAATGATTGT GTGCATATAC TTCATCATCC TTTTCATCTG TGGAAACTAC
    ATTCTCCTGA ATGTGTTCTT GGCTATCGCT GTGGACAATC TTGCTGATGC AGAGTCTCTG
    AACACGGACG ATACGAAGAA ACCTGATGAA ATCGACGAAA TTGAAGATGA AGCCAAGGCT
    GGAGAGGAGG ATGAGAAGGA TAATGCCGAG GAGGATGAAG AAGAGCCAGA TGTTCCTGCA
    GGGCCTCGTC CAAAGATCTC AGAGCTTGTT AAGAAGGAGA AGATCACTCC AATCCCAGAG
    GGCAGTGCGT TCTTCATCTT CAGCAACACA AACCCAGTTC GAGTCGCCTG CCACAAGCTG
    ATAAACCACC ACATCTTCAC CAACCTCATC CTTGTGTTCA TTATGCTAAG CTCAGCGTCA
    TTGGCTGCTG AGGATCCAAT CAGAAACTTC TCTGCTAGGA ATATTATACT TGGTTACTTT
    GACTATGCTT TCACGGCTAT CTTTACTGTA GAGATCGTGT TAAAGATGAC CACGTATGGC
    GCTTTTCTCC ATAAGGGTGC TTTCTGCAGG AACTACTTTA ATCTTCTAGA TCTGCTGGTG
    GTCGGAGTTT CGCTCGTGTC CTTCGGGATT CAATCATCGG CCATCTCAGT GGTGAAGATT
    CTGAGGGTTC TGAGAGTTTT GCGTCCACTC AGAGCCATAA ACAGAGCTAA AGGACTAAAG
    CATGTGGTCC AGTGTGTGTT TGTGGCCATC AGGACTATAG GAAACATCAT GATCGTCACC
    ACTCTGCTGC AGTTTATGTT CGCCTGCATT GGTGTCCAGC TCTTCAAGGG AAAGTTTTAC
    CGTTGTAACG ATGAGGCCAA GTCCAGCCCA GAGGAGTGCA AGGGAACGTA CATCATGTAT
    AAGGAAGGAG ACGTGAACCA GCCAATCATT CAGAAGCGCC ACTGGCACAA CAGTGATTTC
    AACTTTGACA ATGTGCTCAT GGCCATGATG GCTCTGTTCA CCGTGTCCAC GTTTGAAGGC
    TGGCCAGCGT TGCTGTACAA GGCCATCGAC TCCAACCGGG AGAACATGGG TCCCATCTAC
    AACTACCGAG TGGAGATTTC CATCTTCTTC ATCATCTACA TCATCATCAT CGCTTTCTTT
    ATGATGAACA TCTTTGTGGG TTTCGTCATC GTGACCTTTC AGGAACAGGG AGAGAAGGAG
    TACAAGAACT GTGAGCTGGA CAAGAACCAG CGTCAGTGTG TGGAGTATGC ATTGAAAGCA
    CGTCCTCTCA GGAGGTACAT CCCAAAGAAC CCATATCAGT ATAAGTTCTG GTATGTGGTG
    AACTCCACCG GCTTCGAGTA CATCATGTTT GTCCTGATTT TACTCAACAC CATCTGTCTC
    GCCGTGCAGC ACTATGGTCA GTCTGAGCTC TTCAATTATG TTATGGACAT TCTGAATATG
    GTCTTCACTG CCGTCTTCAC TGTGGAGATG GTGCTCAAGC TGATCGCCTT CAAGCCTCGG
    CATTATTTCA CTGACGCATG GAACACATTT GACGCCTTAA TTGTTGTCGG TAGCGTCGTA
    GATATTGCTA TCACTGAAGT CAACAACACT GAGGACAGCG CTCGCATTTC CATCACTTTC
    TTCCGTCTTT TTCGAGTGAT GCGATTGGTC AAGCTGCTGA GCAGAGGGGA GGGAATTCGA
    ACCCTACTCT GGACCTTTAT CAAATCCTTC CAGGCACTGC CTTATGTTGC TTTGCTGATA
    GCCATGCTCT TCTTCATCTA CGCTGTCATT GGCATGCAGG TGTTTGGGAA AATTGCCATG
    GTGGATCACA CTCAAATCAA CAGGAACAAC AACTTTCAGA CGTTCCCTCA GGCTGTGCTT
    CTGCTCTTCA GGTGTGCTAC GGGTGAAGCC TGGCAGGAGA TCATGTTAGC ATGTATGCCA
    GGCAAGCTGT GTGACCCGGA GTCAGACTAT AATCCTGGGG AAGAAATGAC CTGCGGGAGC
    AGTTTCGCCA TCATCTACTT CATCACCTTC TACATGCTCT GCGCCTTCCT GATCATTAAC
    CTGTTTGTGG CTGTCATCAT GGACAACTTC GACTATCTGA CCCGTGATTG GTCAATTCTG
    GGGCCACATC ACCTTGATGA GTTTAAGAGG ATTTGGTCAG AATATGACCC AGAGGCCAAG
    GGCAGAATAA AACATCTTGA TGTAGTGACG TTGTTGCGCC GTATTCAGCC TCCTCTCGGC
    TTTGGGAAAC TCTGTCCTCA TAGAGTCGCC TGCAAGAGGC TGGTGGCTAT GAACATGCCC
    CTGAACAGTG ACGGCACGGT CATGTTTAAC GCGACACTGT TTGCTCTCGT GAGAACCGCA
    TTGAAAATCA AAACAGAAGG TAACCTAGAA CAAGCGAATG AAGAGCTCCG AGCTGTGATT
    AAGAAGATCT GGAAGAGAAC CAGCATGAAG CTTCTGGATC AAGTGGTTCC CCCTGCTGGT
    GATGATGAGG TAACGGTGGG GAAGTTCTAT GCCACTTTCC TGATACAGGA CTACTTTAGG
    AAATTCAAGA AACGCAAAGA GGAAGGCCTG GTGGGAGTTC ATCCGGCACA GAACAACACG
    GCCATCGCAC TGCAGGCTGG CCTTCGTACG CTGCATGACA TTGGGCCAGA AATCCGCCGT
    GCGATATCAT GTGACCTGCA GGATGATGAG CTAGTAGACT TTATACCAGA GGAGGACGAA
    GAAATTTATA GGCGAAACGG AGGACTCTTT GGCAACCACA TCAACCACAT AAACGGAGAC
    CCCCGGAGGA GCTCTGGCCA CCAAACAAAC GCCACACAGC GCCCCCTGCA GGTGCAGCCG
    CCCCCACATT ACGTCCACAT GGAGCAGCCC GTGGGGCGTC TGGGTCGCGC CAATGCCATG
    GCACAGCAAA ACCATCATCG TCACCACCAC CACCATCACC ATCATCATCA TCACAACAAC
    TCCTACAACA AATCGCCCAA GTCCACCAAC ATCAACCTCA ACAACGCCAA CGTGTCCAGT
    TTACCCAATG GTGGACACAA TCGCTACTAC GAACACGCTC CCGCTAATGG CTACCCAGGG
    TCATATTACG GAGAATACGA CAAGCCCAGG ACCCCTCATG GGCAAAGAAG GCGCTACTAT
    GAGACATATA TTAGATCGCA GGGCAGCGAC AGAAGACGAC CCACGATACG CCGCGAAGAG
    GAGTATGAAG AAGATCGCTA CTCTGGAGAG TATTACAGCG GAGAGGAGTT TTATGAAGAT
    GACAGCATGC TATCGGGTGA TAGGTACCCC AACAGTGACC AGGAGTACGA GACGCCGCGG
    GGTTACCATC ATCCTGACAG TTACTATGAA GATGATGAGC AGCCCCTTTA TCACGATTCA
    CATAGGTCAC CAAAGAGAAG ACTGCTTCCT CCAACACCGC AAGGTAATCG GAGACCGTCG
    TTCAATTTTG AGTGTCTGCG CAGACAGAGC AGTCAAGACG ATCTTCCTCA CCAGCGAACT
    GCTCTTCCGT TACATCTCAT GCAACACCAG GTGATGGCAG TGGCGGGTCT GGACTCTAGC
    AGGGCTCACA GGCTTTCACC AACTCGATCT ACCAGATCAT GGGCTTCACC TCCTCCAACT
    CCTGCAAGTA AAGACCGGAC GCCGTACTAC ACACCGCTCA TCCGCGTGGA CCGTCCTCTA
    AGAGACAGCG CCAGCAGCAG CCATTCATCC ATCAGGAAGA GCTCGTGGTA CACAGACGAC
    CCTGAGTACC AGCAGAGGAA CTTCTCACCT GTTCACCTGC AGGTCCCACC CGAGTACAGG
    AACCAGTACC TGCAGAAGAG AGGCAGCGCT ACCAGCCTGG TGGAGGCGGT CTTGATATCA
    GAAGGACTGG GTCGATACGC CAAGGATCCC AAATTTGTGG CCGCCACAAA GCATGAGATT
    GCGGACGCGT GCGAGATGAC CATTGACGAA ATGGAAAGCG CCGCCAGCCA TCTTCTTAAC
    GGTGGCATTA CACCAGTAGT CAACGGAGTC AATGTGTTTC CCATACTGGG CCACAGAGAC
    TACGAGCTGC AGGACGTCTC GGCGAGCTAC AGCGACGAGG AGCCCGAACC CGAGCCCAGA
    CCTCGTTACG AGGAGGACCT TGCAGATGAA ATGATATGCA TTACCACTTT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335259.1
    CDS410..6583
    Translation

    Target ORF information:

    RefSeq Version XM_021479584.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a (cacna1da), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479584.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAGCGCCA ACGGCCCTGC TCCGCCTGCT GCGACTCCTG CCGCGCCCCC AGCAGCTGCC 
    GTGCCTGTAC CTTCGGTGGT ACCGGTCGGA TCGCTGGCCC AGAAGAAGCG GGCGCAGTAT
    GCCAAGAGCA AAAAACAGGG CAGTTCGGCC AATACGCGGC CACAGAGAGC TCTCTTCTGC
    CTCAACCTCA ACAACCCCAT CCGCCGGGCC TGTATCAGCC TTGTGGAATG GAAGCCATTT
    GATATCTTTA TATTGATAGC GATTTTCGCC AACTGCATGG CCTTAGCTGT ATATGTCCCT
    TTCCCCGAGG ATGATTCCAA CTCAACCAAC CACGACCTGG AAACAGTCGA ATATGCCTTC
    CTTATCATTT TCACGATCGA AACCTTCCTG AAGATTATAG CGTACGGCTT GGTGATGCAT
    CAGAACGCTT ATGTCCGGAA TGGATGGAAC ATGCTGGATT TCGTCATTGT CGTCATTGGA
    TTGTTTAGTG TTGTCTTGGA AGTGCTGACC AAAGAAGGGG GTGAGAAGGA AGAAGTTGGA
    GAAAATCTCT CCGCTCATGG CCATGGAGGA AAACCAGGAG GTTTCGATGT TAAAGCCCTC
    AGAGCCTTTC GAGTGCTGCG ACCGCTTCGC CTGGTCTCAG GAGTGCCCAG TTTACAGGTG
    GTGCTGAACT CCATCATTAA AGCCATGGTT CCTCTCCTCC ACATCGCCCT GCTGGTTCTG
    TTTGTCATCA TCATCTATGC TATTATCGGT TTGGAGCTTT TCATTGGAAA AATGCACGCT
    TCCTGTTATT TCCAAGGGAC AGATATTTTA GAGGATGAAC CGGCACCGTG TGCGGTGAAT
    GGTCACGGCC GCACATGCCC CATTAACGGC ACTCTGTGTA AAGAAGGCTG GCAAGGACCC
    AACGGAGGCA TCACCAACTT CGACAACTTC ATGTTCGCCA TGCTCACTGT GTTTCAATGC
    ATTACCATGG AGGGCTGGAC GGACGTGCTT TATTGGATGA ATGATGCTAT GGGTTTGGAG
    CTGCCCTGGG TGTACTTTGT GAGTTTGGTG ATCTTCGGCT CTTTCTTCGT CTTGAACCTG
    GTTCTGGGTG TGTTGAGCGG AGAGTTCTCT AAAGAGCGAG AGAAGGCGAA GGCTCGTGGA
    GATTTCCAGA AGCTTCGAGA GAAACAGCAG CTGGAGGAAG ATCTGAAAGG TTATCTGGAC
    TGGATCACGC AGGCTGAGGA CATCGACCCT GAGAACGAGG AGGAAGAGGA GGAGAGCAAA
    CGCAACCCGA GTATGCCGGC AAGTGAGACT GAATCCATGA ATACAGAGAA CGAGAAAGGA
    GAAGATGAGA AGGCGACATG TTGCGGCCCG ACATGTCAAA AAATATCAAA ATCAAAGTTC
    AGTCGGCGCT GGCGTCGCTG GAACCGGTTG TGTCGGAGGA ATTGTCGCTT GGCTGTAAAA
    TCTGTGCCAT TCTATTGGCT GGTAATCATC CTGGTGTTCC TGAACACTTT GACCATCTCT
    TCAGAACATT ACAACCAGCC CATGTGGCTC ACACAAGTAC AGGATGTGGC TAATAAAGTG
    CTGCTGGCCA TGTTCACGTG TGAGATGTTG GTGAAGATGT ACAGTCTGGG CCTGCAGGCT
    TATTTTGTGT CTCTTTTTAA TCGCTTTGAC TGCTTTGTGG TGTGTGGAGG CATCACTGAA
    ACCATCCTTG TGGAGTTTGA GATTATGTCA CCCCTCGGCA TCTCAGTGTT TCGATGTGTT
    CGACTTCTCA GGATCTTTAA AGTTACACGC CACTGGGCGT CTCTCAGTAA CCTGGTGGCA
    TCTCTGCTGA ACTCCATGAA GTCCATTGCT TCTCTACTGC TGCTGCTCTT CCTCTTCATC
    ATCATCTTTA GCCTCCTCGG TATGCAGGTG TTCGGCGGCA AGTTTAACTT TGACGAGACA
    CAAACCAAGA GGAGCACCTT TGACAACTTC CCTCAGGCCT TGCTCACTGT GTTTCAGATT
    CTAACTGGTG AAGACTGGAA TGCCGTCATG TATGACGGAA TTATGGCGTA CGGCGGCCCT
    TCGTCATCAG GAATGATTGT GTGCATATAC TTCATCATCC TTTTCATCTG TGGAAACTAC
    ATTCTCCTGA ATGTGTTCTT GGCTATCGCT GTGGACAATC TTGCTGATGC AGAGTCTCTG
    AACACGGACG ATACGAAGAA ACCTGATGAA ATCGACGAAA TTGAAGATGA AGCCAAGGCT
    GGAGAGGAGG ATGAGAAGGA TAATGCCGAG GAGGATGAAG AAGAGCCAGA TGTTCCTGCA
    GGGCCTCGTC CAAAGATCTC AGAGCTTGTT AAGAAGGAGA AGATCACTCC AATCCCAGAG
    GGCAGTGCGT TCTTCATCTT CAGCAACACA AACCCAGTTC GAGTCGCCTG CCACAAGCTG
    ATAAACCACC ACATCTTCAC CAACCTCATC CTTGTGTTCA TTATGCTAAG CTCAGCGTCA
    TTGGCTGCTG AGGATCCAAT CAGAAACTTC TCTGCTAGGA ATATTATACT TGGTTACTTT
    GACTATGCTT TCACGGCTAT CTTTACTGTA GAGATCGTGT TAAAGATGAC CACGTATGGC
    GCTTTTCTCC ATAAGGGTGC TTTCTGCAGG AACTACTTTA ATCTTCTAGA TCTGCTGGTG
    GTCGGAGTTT CGCTCGTGTC CTTCGGGATT CAATCATCGG CCATCTCAGT GGTGAAGATT
    CTGAGGGTTC TGAGAGTTTT GCGTCCACTC AGAGCCATAA ACAGAGCTAA AGGACTAAAG
    CATGTGGTCC AGTGTGTGTT TGTGGCCATC AGGACTATAG GAAACATCAT GATCGTCACC
    ACTCTGCTGC AGTTTATGTT CGCCTGCATT GGTGTCCAGC TCTTCAAGGG AAAGTTTTAC
    CGTTGTAACG ATGAGGCCAA GTCCAGCCCA GAGGAGTGCA AGGGAACGTA CATCATGTAT
    AAGGAAGGAG ACGTGAACCA GCCAATCATT CAGAAGCGCC ACTGGCACAA CAGTGATTTC
    AACTTTGACA ATGTGCTCAT GGCCATGATG GCTCTGTTCA CCGTGTCCAC GTTTGAAGGC
    TGGCCAGCGT TGCTGTACAA GGCCATCGAC TCCAACCGGG AGAACATGGG TCCCATCTAC
    AACTACCGAG TGGAGATTTC CATCTTCTTC ATCATCTACA TCATCATCAT CGCTTTCTTT
    ATGATGAACA TCTTTGTGGG TTTCGTCATC GTGACCTTTC AGGAACAGGG AGAGAAGGAG
    TACAAGAACT GTGAGCTGGA CAAGAACCAG CGTCAGTGTG TGGAGTATGC ATTGAAAGCA
    CGTCCTCTCA GGAGGTACAT CCCAAAGAAC CCATATCAGT ATAAGTTCTG GTATGTGGTG
    AACTCCACCG GCTTCGAGTA CATCATGTTT GTCCTGATTT TACTCAACAC CATCTGTCTC
    GCCGTGCAGC ACTATGGTCA GTCTGAGCTC TTCAATTATG TTATGGACAT TCTGAATATG
    GTCTTCACTG CCGTCTTCAC TGTGGAGATG GTGCTCAAGC TGATCGCCTT CAAGCCTCGG
    CATTATTTCA CTGACGCATG GAACACATTT GACGCCTTAA TTGTTGTCGG TAGCGTCGTA
    GATATTGCTA TCACTGAAGT CAACAACACT GAGGACAGCG CTCGCATTTC CATCACTTTC
    TTCCGTCTTT TTCGAGTGAT GCGATTGGTC AAGCTGCTGA GCAGAGGGGA GGGAATTCGA
    ACCCTACTCT GGACCTTTAT CAAATCCTTC CAGGCACTGC CTTATGTTGC TTTGCTGATA
    GCCATGCTCT TCTTCATCTA CGCTGTCATT GGCATGCAGG TGTTTGGGAA AATTGCCATG
    GTGGATCACA CTCAAATCAA CAGGAACAAC AACTTTCAGA CGTTCCCTCA GGCTGTGCTT
    CTGCTCTTCA GGTGTGCTAC GGGTGAAGCC TGGCAGGAGA TCATGTTAGC ATGTATGCCA
    GGCAAGCTGT GTGACCCGGA GTCAGACTAT AATCCTGGGG AAGAAATGAC CTGCGGGAGC
    AGTTTCGCCA TCATCTACTT CATCACCTTC TACATGCTCT GCGCCTTCCT GATCATTAAC
    CTGTTTGTGG CTGTCATCAT GGACAACTTC GACTATCTGA CCCGTGATTG GTCAATTCTG
    GGGCCACATC ACCTTGATGA GTTTAAGAGG ATTTGGTCAG AATATGACCC AGAGGCCAAG
    GGCAGAATAA AACATCTTGA TGTAGTGACG TTGTTGCGCC GTATTCAGCC TCCTCTCGGC
    TTTGGGAAAC TCTGTCCTCA TAGAGTCGCC TGCAAGAGGC TGGTGGCTAT GAACATGCCC
    CTGAACAGTG ACGGCACGGT CATGTTTAAC GCGACACTGT TTGCTCTCGT GAGAACCGCA
    TTGAAAATCA AAACAGAAGG TAACCTAGAA CAAGCGAATG AAGAGCTCCG AGCTGTGATT
    AAGAAGATCT GGAAGAGAAC CAGCATGAAG CTTCTGGATC AAGTGGTTCC CCCTGCTGGT
    GATGATGAGG TAACGGTGGG GAAGTTCTAT GCCACTTTCC TGATACAGGA CTACTTTAGG
    AAATTCAAGA AACGCAAAGA GGAAGGCCTG GTGGGAGTTC ATCCGGCACA GAACAACACG
    GCCATCGCAC TGCAGGCTGG CCTTCGTACG CTGCATGACA TTGGGCCAGA AATCCGCCGT
    GCGATATCAT GTGACCTGCA GGATGATGAG CTAGTAGACT TTATACCAGA GGAGGACGAA
    GAAATTTATA GGCGAAACGG AGGACTCTTT GGCAACCACA TCAACCACAT AAACGGAGAC
    CCCCGGAGGA GCTCTGGCCA CCAAACAAAC GCCACACAGC GCCCCCTGCA GGTGCAGCCG
    CCCCCACATT ACGTCCACAT GGAGCAGCCC GTGGGGCGTC TGGGTCGCGC CAATGCCATG
    GCACAGCAAA ACCATCATCG TCACCACCAC CACCATCACC ATCATCATCA TCACAACAAC
    TCCTACAACA AATCGCCCAA GTCCACCAAC ATCAACCTCA ACAACGCCAA CGTGTCCAGT
    TTACCCAATG GTGGACACAA TCGCTACTAC GAACACGCTC CCGCTAATGG CTACCCAGGG
    TCATATTACG GAGAATACGA CAAGCCCAGG ACCCCTCATG GGCAAAGAAG GCGCTACTAT
    GAGACATATA TTAGATCGCA GGGCAGCGAC AGAAGACGAC CCACGATACG CCGCGAAGAG
    GAGTATGAAG AAGATCGCTA CTCTGGAGAG TATTACAGCG GAGAGGAGTT TTATGAAGAT
    GACAGCATGC TATCGGGTGA TAGGTACCCC AACAGTGACC AGGAGTACGA GACGCCGCGG
    GGTTACCATC ATCCTGACAG TTACTATGAA GATGATGAGC AGCCCCTTTA TCACGATTCA
    CATAGGTCAC CAAAGAGAAG ACTGCTTCCT CCAACACCGC AAGGTAATCG GAGACCGTCG
    TTCAATTTTG AGTGTCTGCG CAGACAGAGC AGTCAAGACG ATCTTCCTCA CCAGCGAACT
    GCTCTTCCGT TACATCTCAT GCAACACCAG GTGATGGCAG TGGCGGGTCT GGACTCTAGC
    AGGGCTCACA GGCTTTCACC AACTCGATCT ACCAGATCAT GGGCTTCACC TCCTCCAACT
    CCTGCAAGTA AAGACCGGAC GCCGTACTAC ACACCGCTCA TCCGCGTGGA CCGTCCTCTA
    AGAGACAGCG CCAGCAGCAG CCATTCATCC ATCAGGAAGA GCTCGTGGTA CACAGACGAC
    CCTGAGTACC AGCAGAGGAA CTTCTCACCT GTTCACCTGC AGGTCCCACC CGAGTACAGG
    AACCAGTACC TGCAGAAGAG AGGCAGCGCT ACCAGCCTGG TGGAGGCGGT CTTGATATCA
    GAAGGACTGG GTCGATACGC CAAGGATCCC AAATTTGTGG CCGCCACAAA GCATGAGATT
    GCGGACGCGT GCGAGATGAC CATTGACGAA ATGGAAAGCG CCGCCAGCCA TCTTCTTAAC
    GGTGGCATTA CACCAGTAGT CAACGGAGTC AATGTGTTTC CCATACTGGG CCACAGAGAC
    TACGAGCTGC AGGACGTCTC GGCGAGCTAC AGCGACGAGG AGCCCGAACC CGAGCCCAGA
    CCTCGTTACG AGGAGGACCT TGCAGATGAA ATGATATGCA TTACCACTTT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.