Cacna1d gene cDNA ORF clone, Rattus norvegicus(Norway rat)

mRNA	Protein	Name
XM_017600057.1	XP_017455546.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
XM_017600058.1	XP_017455547.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
XM_017600059.1	XP_017455548.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
XM_017600060.1	XP_017455549.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
XM_017600064.1	XP_017455553.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
XM_006252597.3	XP_006252659.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
XM_017600066.1	XP_017455555.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
XM_008770992.2	XP_008769214.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X19
XM_017600056.1	XP_017455545.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
XM_017600063.1	XP_017455552.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
XM_006252594.3	XP_006252656.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X12
XM_017600065.1	XP_017455554.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
XM_017600068.1	XP_017455557.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X20
NM_017298.1	NP_058994.1	voltage-dependent L-type calcium channel subunit alpha-1D
XM_017600055.1	XP_017455544.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
XM_006252590.3	XP_006252652.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
XM_017600069.1	XP_017455558.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X21
XM_017600067.1	XP_017455556.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X17
XM_008770991.2	XP_008769213.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X18
XM_017600061.1	XP_017455550.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
XM_017600062.1	XP_017455551.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
XM_006252595.3	XP_006252657.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
XM_017600070.1	XP_017455559.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X22

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa58789	XM_017600066.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa58790	XM_017600067.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa43354	XM_008770991.2 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa43355	XM_008770992.2 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa58778	XM_017600055.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa58779	XM_017600056.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa58780	XM_017600057.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa58781	XM_017600058.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa58782	XM_017600059.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa58783	XM_017600060.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa58784	XM_017600061.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa58785	XM_017600062.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa58786	XM_017600063.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa58787	XM_017600064.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa58788	XM_017600065.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa58791	XM_017600068.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa58792	XM_017600069.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa58793	XM_017600070.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa12532	NM_017298.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa37028	XM_006252590.3 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa37032	XM_006252594.3 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa37033	XM_006252595.3 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa37035	XM_006252597.3 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

Cacna1d ( XM_017600066.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455555.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X16, mRNA.

Price & Availability↑

CloneID	ORa58789
Clone ID Related Accession (Same CDS sequence)	XM_017600066.1
Accession Version	XM_017600066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GACAGTGAGA ATATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAGCT TCTCAGCAGA 
GGGGAAGGCA TCCGGACTCT GCTATGGACC TTCATTAAGT CCTTCCAGGC ACTCCCATAT 
GTCGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCCG TCATTGGCAT GCAGATGTTT 
GGGAAGGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACGTTT 
CCCCAGGCAG TGCTGCTGCT CTTCAGGTGT GCAACAGGGG AGGCCTGGCA GGAGATCATG 
CTCGCCTGCC TCCCTGGGAA GCTGTGTGAC CCGGACTCAG ATTACAACCC AGGAGAGGAA 
TATACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCG 
TTCCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACGAGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCTGAATAT 
GACCCCGAGG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTTTGCT CCGACGTATC 
CAGCCTCCCC TGGGATTTGG AAAGTTGTGT CCACACCGAG TGGCGTGTAA GAGATTGGTC 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC TCTGTTTGCT 
TTGGTACGGA CGGCTCTCAA GATCAAGACT GAAGGCAACC TGGAGCAAGC TAATGAAGAG 
CTCCGCGCTG TGATAAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTACT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAGCGG AAAGAGCAAG GCCTGGTGGG GAAATACCCT 
GCGAAGAACA CCACGATCGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AACCAGAAGA CTCCAAACCA 
GAAGAGGAAG ATGTATTCAA ACAGGAAATT CGGTGTGTCA TAACCATCAT AACCATAATT 
CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455555.1
CDS	418..5643
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSDSENIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKQEIRCVITIITIIP

Target ORF information:

RefSeq Version	XM_017600066.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600066.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GACAGTGAGA ATATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAGCT TCTCAGCAGA 
GGGGAAGGCA TCCGGACTCT GCTATGGACC TTCATTAAGT CCTTCCAGGC ACTCCCATAT 
GTCGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCCG TCATTGGCAT GCAGATGTTT 
GGGAAGGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACGTTT 
CCCCAGGCAG TGCTGCTGCT CTTCAGGTGT GCAACAGGGG AGGCCTGGCA GGAGATCATG 
CTCGCCTGCC TCCCTGGGAA GCTGTGTGAC CCGGACTCAG ATTACAACCC AGGAGAGGAA 
TATACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCG 
TTCCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACGAGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCTGAATAT 
GACCCCGAGG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTTTGCT CCGACGTATC 
CAGCCTCCCC TGGGATTTGG AAAGTTGTGT CCACACCGAG TGGCGTGTAA GAGATTGGTC 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC TCTGTTTGCT 
TTGGTACGGA CGGCTCTCAA GATCAAGACT GAAGGCAACC TGGAGCAAGC TAATGAAGAG 
CTCCGCGCTG TGATAAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTACT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAGCGG AAAGAGCAAG GCCTGGTGGG GAAATACCCT 
GCGAAGAACA CCACGATCGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AACCAGAAGA CTCCAAACCA 
GAAGAGGAAG ATGTATTCAA ACAGGAAATT CGGTGTGTCA TAACCATCAT AACCATAATT 
CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600067.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455556.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X17, mRNA.

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CloneID	ORa58790
Clone ID Related Accession (Same CDS sequence)	XM_017600067.1
Accession Version	XM_017600067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GACAGTGAGA ATATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAGCT TCTCAGCAGA 
GGGGAAGGCA TCCGGACTCT GCTATGGACC TTCATTAAGT CCTTCCAGGC ACTCCCATAT 
GTCGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCCG TCATTGGCAT GCAGATGTTT 
GGGAAGGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACGTTT 
CCCCAGGCAG TGCTGCTGCT CTTCAGGTGT GCAACAGGGG AGGCCTGGCA GGAGATCATG 
CTCGCCTGCC TCCCTGGGAA GCTGTGTGAC CCGGACTCAG ATTACAACCC AGGAGAGGAA 
TATACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCG 
TTCCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACGAGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCTGAATAT 
GACCCCGAGG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTTTGCT CCGACGTATC 
CAGCCTCCCC TGGGATTTGG AAAGTTGTGT CCACACCGAG TGGCGTGTAA GAGATTGGTC 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC TCTGTTTGCT 
TTGGTACGGA CGGCTCTCAA GATCAAGACT GAAGGCAACC TGGAGCAAGC TAATGAAGAG 
CTCCGCGCTG TGATAAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTACT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAGCGG AAAGAGCAAG GCCTGGTGGG GAAATACCCT 
GCGAAGAACA CCACGATCGC CCTACAGATG CTTGAACGGA TGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455556.1
CDS	418..5505
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSDSENIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQMLERML

Target ORF information:

RefSeq Version	XM_017600067.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600067.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GACAGTGAGA ATATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAGCT TCTCAGCAGA 
GGGGAAGGCA TCCGGACTCT GCTATGGACC TTCATTAAGT CCTTCCAGGC ACTCCCATAT 
GTCGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCCG TCATTGGCAT GCAGATGTTT 
GGGAAGGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACGTTT 
CCCCAGGCAG TGCTGCTGCT CTTCAGGTGT GCAACAGGGG AGGCCTGGCA GGAGATCATG 
CTCGCCTGCC TCCCTGGGAA GCTGTGTGAC CCGGACTCAG ATTACAACCC AGGAGAGGAA 
TATACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCG 
TTCCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACGAGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCTGAATAT 
GACCCCGAGG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTTTGCT CCGACGTATC 
CAGCCTCCCC TGGGATTTGG AAAGTTGTGT CCACACCGAG TGGCGTGTAA GAGATTGGTC 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC TCTGTTTGCT 
TTGGTACGGA CGGCTCTCAA GATCAAGACT GAAGGCAACC TGGAGCAAGC TAATGAAGAG 
CTCCGCGCTG TGATAAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTACT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAGCGG AAAGAGCAAG GCCTGGTGGG GAAATACCCT 
GCGAAGAACA CCACGATCGC CCTACAGATG CTTGAACGGA TGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_008770991.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769213.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X18, mRNA.

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CloneID	ORa43354
Clone ID Related Accession (Same CDS sequence)	XM_008770991.2
Accession Version	XM_008770991.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAT ACTTTTGATG CCTTAATTGT TGTCGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAGC TTCTCAGCAG AGGGGAAGGC 
ATCCGGACTC TGCTATGGAC CTTCATTAAG TCCTTCCAGG CACTCCCATA TGTCGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCC GTCATTGGCA TGCAGATGTT TGGGAAGGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACGTT TCCCCAGGCA 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGG GAGGCCTGGC AGGAGATCAT GCTCGCCTGC 
CTCCCTGGGA AGCTGTGTGA CCCGGACTCA GATTACAACC CAGGAGAGGA ATATACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC GTTCCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACGAG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCTGAATA TGACCCCGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTTTGC TCCGACGTAT CCAGCCTCCC 
CTGGGATTTG GAAAGTTGTG TCCACACCGA GTGGCGTGTA AGAGATTGGT CGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CTCTGTTTGC TTTGGTACGG 
ACGGCTCTCA AGATCAAGAC TGAAGGCAAC CTGGAGCAAG CTAATGAAGA GCTCCGCGCT 
GTGATAAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAGCG GAAAGAGCAA GGCCTGGTGG GGAAATACCC TGCGAAGAAC 
ACCACGATCG CCCTACAGAT GCTTGAACGG ATGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769213.1
CDS	418..5376
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQMLERML

Target ORF information:

RefSeq Version	XM_008770991.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770991.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAT ACTTTTGATG CCTTAATTGT TGTCGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAGC TTCTCAGCAG AGGGGAAGGC 
ATCCGGACTC TGCTATGGAC CTTCATTAAG TCCTTCCAGG CACTCCCATA TGTCGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCC GTCATTGGCA TGCAGATGTT TGGGAAGGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACGTT TCCCCAGGCA 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGG GAGGCCTGGC AGGAGATCAT GCTCGCCTGC 
CTCCCTGGGA AGCTGTGTGA CCCGGACTCA GATTACAACC CAGGAGAGGA ATATACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC GTTCCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACGAG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCTGAATA TGACCCCGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTTTGC TCCGACGTAT CCAGCCTCCC 
CTGGGATTTG GAAAGTTGTG TCCACACCGA GTGGCGTGTA AGAGATTGGT CGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CTCTGTTTGC TTTGGTACGG 
ACGGCTCTCA AGATCAAGAC TGAAGGCAAC CTGGAGCAAG CTAATGAAGA GCTCCGCGCT 
GTGATAAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAGCG GAAAGAGCAA GGCCTGGTGG GGAAATACCC TGCGAAGAAC 
ACCACGATCG CCCTACAGAT GCTTGAACGG ATGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_008770992.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769214.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X19, mRNA.

Price & Availability↑

CloneID	ORa43355
Clone ID Related Accession (Same CDS sequence)	XM_008770992.2
Accession Version	XM_008770992.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CCATCTGACA GTGAGAATAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAGCTTCTC AGCAGAGGGG AAGGCATCCG GACTCTGCTA 
TGGACCTTCA TTAAGTCCTT CCAGGCACTC CCATATGTCG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCCGTCAT TGGCATGCAG ATGTTTGGGA AGGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACGTTTCCCC AGGCAGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGGGAGGC CTGGCAGGAG ATCATGCTCG CCTGCCTCCC TGGGAAGCTG 
TGTGACCCGG ACTCAGATTA CAACCCAGGA GAGGAATATA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCGTTCC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACGAGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCT GAATATGACC CCGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TTTGCTCCGA CGTATCCAGC CTCCCCTGGG ATTTGGAAAG 
TTGTGTCCAC ACCGAGTGGC GTGTAAGAGA TTGGTCGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACTCTG TTTGCTTTGG TACGGACGGC TCTCAAGATC 
AAGACTGAAG GCAACCTGGA GCAAGCTAAT GAAGAGCTCC GCGCTGTGAT AAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAGCGGAAAG AGCAAGGCCT GGTGGGGAAA TACCCTGCGA AGAACACCAC GATCGCCCTA 
CAGATGCTTG AACGGATGCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769214.1
CDS	418..5361
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPSDSENIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQMLERML

Target ORF information:

RefSeq Version	XM_008770992.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770992.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CCATCTGACA GTGAGAATAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAGCTTCTC AGCAGAGGGG AAGGCATCCG GACTCTGCTA 
TGGACCTTCA TTAAGTCCTT CCAGGCACTC CCATATGTCG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCCGTCAT TGGCATGCAG ATGTTTGGGA AGGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACGTTTCCCC AGGCAGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGGGAGGC CTGGCAGGAG ATCATGCTCG CCTGCCTCCC TGGGAAGCTG 
TGTGACCCGG ACTCAGATTA CAACCCAGGA GAGGAATATA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCGTTCC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACGAGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCT GAATATGACC CCGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TTTGCTCCGA CGTATCCAGC CTCCCCTGGG ATTTGGAAAG 
TTGTGTCCAC ACCGAGTGGC GTGTAAGAGA TTGGTCGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACTCTG TTTGCTTTGG TACGGACGGC TCTCAAGATC 
AAGACTGAAG GCAACCTGGA GCAAGCTAAT GAAGAGCTCC GCGCTGTGAT AAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAGCGGAAAG AGCAAGGCCT GGTGGGGAAA TACCCTGCGA AGAACACCAC GATCGCCCTA 
CAGATGCTTG AACGGATGCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600055.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455544.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X1, mRNA.

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CloneID	ORa58778
Clone ID Related Accession (Same CDS sequence)	XM_017600055.1
Accession Version	XM_017600055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GACAGTGAGA ATATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAGCT TCTCAGCAGA 
GGGGAAGGCA TCCGGACTCT GCTATGGACC TTCATTAAGT CCTTCCAGGC ACTCCCATAT 
GTCGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCCG TCATTGGCAT GCAGATGTTT 
GGGAAGGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACGTTT 
CCCCAGGCAG TGCTGCTGCT CTTCAGGTGT GCAACAGGGG AGGCCTGGCA GGAGATCATG 
CTCGCCTGCC TCCCTGGGAA GCTGTGTGAC CCGGACTCAG ATTACAACCC AGGAGAGGAA 
TATACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCG 
TTCCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACGAGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCTGAATAT 
GACCCCGAGG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTTTGCT CCGACGTATC 
CAGCCTCCCC TGGGATTTGG AAAGTTGTGT CCACACCGAG TGGCGTGTAA GAGATTGGTC 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC TCTGTTTGCT 
TTGGTACGGA CGGCTCTCAA GATCAAGACT GAAGGCAACC TGGAGCAAGC TAATGAAGAG 
CTCCGCGCTG TGATAAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTACT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAGCGG AAAGAGCAAG GCCTGGTGGG GAAATACCCT 
GCGAAGAACA CCACGATCGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AACCAGAAGA CTCCAAACCA 
GAAGAGGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACTATGT CAATCATGTT 
AATAGTGATA GGAGAGAGTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATTCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGTGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGT TCCCACCTCC 
ACAAATGCCA ATCTCAATAA CGCCAATATG TCCAAAGCTG CCCACGGAAA GCGGCCCAGC 
ATTGGGGACC TTGAGCATGT GTCTGAAAAC GGCCATTACT CCTACAAGCA CGACCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTATG AAACTTACAT TAGGTCTGAG 
TCAGGAGACG AGCAGCTTCC AACCATTTGC CGTGAAGACC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTCGG GGAGCAGGAA TATTTCAGTA GCGAGGAGTG CTGTGAGGAT 
GACAGTTCTC CCACCTGGAG CAGGCAAAAC TACAGCTATT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CATCACCCCC AAGGTTTCTT AGAAGATGAC 
GACTCTCCCA TTGGCTATGA CTCACGGAGA TCTCCAAGAA GACGCCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAACAGCCAA 
GATGACGTCC TTCCGTCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTTATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCGCCG 
AGCCACTCTA CCCGGTCATG GGCCACCCCT CCAGCAACCC CTCCGTACCG AGACTGGACC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAT CGCTCAGAGT CTATGGACCA GGTCAATGGC 
AGCCTGCCGT CGCTACACCG CAGTTCCTGG TATACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACAC CGGCCAGCCT GACCGTCCCC AGCAGCTTCC GGAACAAAAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAAGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCAAGGGACC CAAAATTTGT CTCAGCAACA AAACATGAGA TCGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAGAG CGCGGCCAGC ACCCTGCTCA ATGGAAGCGT GTGTCCTCGA 
GCCAACGGGG ACATGGGCCC CATCTCACAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA ACCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAA 
ATGATTTGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455544.1
CDS	418..7041
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSDSENIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017600055.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600055.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GACAGTGAGA ATATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAGCT TCTCAGCAGA 
GGGGAAGGCA TCCGGACTCT GCTATGGACC TTCATTAAGT CCTTCCAGGC ACTCCCATAT 
GTCGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCCG TCATTGGCAT GCAGATGTTT 
GGGAAGGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACGTTT 
CCCCAGGCAG TGCTGCTGCT CTTCAGGTGT GCAACAGGGG AGGCCTGGCA GGAGATCATG 
CTCGCCTGCC TCCCTGGGAA GCTGTGTGAC CCGGACTCAG ATTACAACCC AGGAGAGGAA 
TATACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCG 
TTCCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACGAGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCTGAATAT 
GACCCCGAGG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTTTGCT CCGACGTATC 
CAGCCTCCCC TGGGATTTGG AAAGTTGTGT CCACACCGAG TGGCGTGTAA GAGATTGGTC 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC TCTGTTTGCT 
TTGGTACGGA CGGCTCTCAA GATCAAGACT GAAGGCAACC TGGAGCAAGC TAATGAAGAG 
CTCCGCGCTG TGATAAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTACT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAGCGG AAAGAGCAAG GCCTGGTGGG GAAATACCCT 
GCGAAGAACA CCACGATCGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AACCAGAAGA CTCCAAACCA 
GAAGAGGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACTATGT CAATCATGTT 
AATAGTGATA GGAGAGAGTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATTCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGTGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGT TCCCACCTCC 
ACAAATGCCA ATCTCAATAA CGCCAATATG TCCAAAGCTG CCCACGGAAA GCGGCCCAGC 
ATTGGGGACC TTGAGCATGT GTCTGAAAAC GGCCATTACT CCTACAAGCA CGACCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTATG AAACTTACAT TAGGTCTGAG 
TCAGGAGACG AGCAGCTTCC AACCATTTGC CGTGAAGACC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTCGG GGAGCAGGAA TATTTCAGTA GCGAGGAGTG CTGTGAGGAT 
GACAGTTCTC CCACCTGGAG CAGGCAAAAC TACAGCTATT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CATCACCCCC AAGGTTTCTT AGAAGATGAC 
GACTCTCCCA TTGGCTATGA CTCACGGAGA TCTCCAAGAA GACGCCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAACAGCCAA 
GATGACGTCC TTCCGTCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTTATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCGCCG 
AGCCACTCTA CCCGGTCATG GGCCACCCCT CCAGCAACCC CTCCGTACCG AGACTGGACC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAT CGCTCAGAGT CTATGGACCA GGTCAATGGC 
AGCCTGCCGT CGCTACACCG CAGTTCCTGG TATACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACAC CGGCCAGCCT GACCGTCCCC AGCAGCTTCC GGAACAAAAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAAGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCAAGGGACC CAAAATTTGT CTCAGCAACA AAACATGAGA TCGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAGAG CGCGGCCAGC ACCCTGCTCA ATGGAAGCGT GTGTCCTCGA 
GCCAACGGGG ACATGGGCCC CATCTCACAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA ACCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAA 
ATGATTTGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600056.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455545.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X2, mRNA.

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CloneID	ORa58779
Clone ID Related Accession (Same CDS sequence)	XM_017600056.1
Accession Version	XM_017600056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTGCTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GACAGTGAGA ATATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAGCT TCTCAGCAGA 
GGGGAAGGCA TCCGGACTCT GCTATGGACC TTCATTAAGT CCTTCCAGGC ACTCCCATAT 
GTCGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCCG TCATTGGCAT GCAGATGTTT 
GGGAAGGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACGTTT 
CCCCAGGCAG TGCTGCTGCT CTTCAGGTGT GCAACAGGGG AGGCCTGGCA GGAGATCATG 
CTCGCCTGCC TCCCTGGGAA GCTGTGTGAC CCGGACTCAG ATTACAACCC AGGAGAGGAA 
TATACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCG 
TTCCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACGAGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCTGAATAT 
GACCCCGAGG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTTTGCT CCGACGTATC 
CAGCCTCCCC TGGGATTTGG AAAGTTGTGT CCACACCGAG TGGCGTGTAA GAGATTGGTC 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC TCTGTTTGCT 
TTGGTACGGA CGGCTCTCAA GATCAAGACT GAAGGCAACC TGGAGCAAGC TAATGAAGAG 
CTCCGCGCTG TGATAAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTACT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAGCGG AAAGAGCAAG GCCTGGTGGG GAAATACCCT 
GCGAAGAACA CCACGATCGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AACCAGAAGA CTCCAAACCA 
GAAGAGGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACTATGT CAATCATGTT 
AATAGTGATA GGAGAGAGTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATTCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGTGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGT TCCCACCTCC 
ACAAATGCCA ATCTCAATAA CGCCAATATG TCCAAAGCTG CCCACGGAAA GCGGCCCAGC 
ATTGGGGACC TTGAGCATGT GTCTGAAAAC GGCCATTACT CCTACAAGCA CGACCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTATG AAACTTACAT TAGGTCTGAG 
TCAGGAGACG AGCAGCTTCC AACCATTTGC CGTGAAGACC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTCGG GGAGCAGGAA TATTTCAGTA GCGAGGAGTG CTGTGAGGAT 
GACAGTTCTC CCACCTGGAG CAGGCAAAAC TACAGCTATT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CATCACCCCC AAGGTTTCTT AGAAGATGAC 
GACTCTCCCA TTGGCTATGA CTCACGGAGA TCTCCAAGAA GACGCCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAACAGCCAA 
GATGACGTCC TTCCGTCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTTATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCGCCG 
AGCCACTCTA CCCGGTCATG GGCCACCCCT CCAGCAACCC CTCCGTACCG AGACTGGACC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAT CGCTCAGAGT CTATGGACCA GGTCAATGGC 
AGCCTGCCGT CGCTACACCG CAGTTCCTGG TATACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACAC CGGCCAGCCT GACCGTCCCC AGCAGCTTCC GGAACAAAAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAAGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCAAGGGACC CAAAATTTGT CTCAGCAACA AAACATGAGA TCGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAGAG CGCGGCCAGC ACCCTGCTCA ATGGAAGCGT GTGTCCTCGA 
GCCAACGGGG ACATGGGCCC CATCTCACAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA ACCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAA 
ATGATTTGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455545.1
CDS	418..7041
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSDSENIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017600056.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600056.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTGCTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GACAGTGAGA ATATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAGCT TCTCAGCAGA 
GGGGAAGGCA TCCGGACTCT GCTATGGACC TTCATTAAGT CCTTCCAGGC ACTCCCATAT 
GTCGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCCG TCATTGGCAT GCAGATGTTT 
GGGAAGGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACGTTT 
CCCCAGGCAG TGCTGCTGCT CTTCAGGTGT GCAACAGGGG AGGCCTGGCA GGAGATCATG 
CTCGCCTGCC TCCCTGGGAA GCTGTGTGAC CCGGACTCAG ATTACAACCC AGGAGAGGAA 
TATACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCG 
TTCCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACGAGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCTGAATAT 
GACCCCGAGG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTTTGCT CCGACGTATC 
CAGCCTCCCC TGGGATTTGG AAAGTTGTGT CCACACCGAG TGGCGTGTAA GAGATTGGTC 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC TCTGTTTGCT 
TTGGTACGGA CGGCTCTCAA GATCAAGACT GAAGGCAACC TGGAGCAAGC TAATGAAGAG 
CTCCGCGCTG TGATAAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTACT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAGCGG AAAGAGCAAG GCCTGGTGGG GAAATACCCT 
GCGAAGAACA CCACGATCGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AACCAGAAGA CTCCAAACCA 
GAAGAGGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACTATGT CAATCATGTT 
AATAGTGATA GGAGAGAGTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATTCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGTGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGT TCCCACCTCC 
ACAAATGCCA ATCTCAATAA CGCCAATATG TCCAAAGCTG CCCACGGAAA GCGGCCCAGC 
ATTGGGGACC TTGAGCATGT GTCTGAAAAC GGCCATTACT CCTACAAGCA CGACCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTATG AAACTTACAT TAGGTCTGAG 
TCAGGAGACG AGCAGCTTCC AACCATTTGC CGTGAAGACC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTCGG GGAGCAGGAA TATTTCAGTA GCGAGGAGTG CTGTGAGGAT 
GACAGTTCTC CCACCTGGAG CAGGCAAAAC TACAGCTATT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CATCACCCCC AAGGTTTCTT AGAAGATGAC 
GACTCTCCCA TTGGCTATGA CTCACGGAGA TCTCCAAGAA GACGCCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAACAGCCAA 
GATGACGTCC TTCCGTCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTTATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCGCCG 
AGCCACTCTA CCCGGTCATG GGCCACCCCT CCAGCAACCC CTCCGTACCG AGACTGGACC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAT CGCTCAGAGT CTATGGACCA GGTCAATGGC 
AGCCTGCCGT CGCTACACCG CAGTTCCTGG TATACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACAC CGGCCAGCCT GACCGTCCCC AGCAGCTTCC GGAACAAAAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAAGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCAAGGGACC CAAAATTTGT CTCAGCAACA AAACATGAGA TCGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAGAG CGCGGCCAGC ACCCTGCTCA ATGGAAGCGT GTGTCCTCGA 
GCCAACGGGG ACATGGGCCC CATCTCACAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA ACCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAA 
ATGATTTGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600057.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455546.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X3, mRNA.

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CloneID	ORa58780
Clone ID Related Accession (Same CDS sequence)	XM_017600057.1
Accession Version	XM_017600057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GACAGTGAGA ATATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAGCT TCTCAGCAGA 
GGGGAAGGCA TCCGGACTCT GCTATGGACC TTCATTAAGT CCTTCCAGGC ACTCCCATAT 
GTCGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCCG TCATTGGCAT GCAGATGTTT 
GGGAAGGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACGTTT 
CCCCAGGCAG TGCTGCTGCT CTTCAGGTGT GCAACAGGGG AGGCCTGGCA GGAGATCATG 
CTCGCCTGCC TCCCTGGGAA GCTGTGTGAC CCGGACTCAG ATTACAACCC AGGAGAGGAA 
TATACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCG 
TTCCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACGAGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCTGAATAT 
GACCCCGAGG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTTTGCT CCGACGTATC 
CAGCCTCCCC TGGGATTTGG AAAGTTGTGT CCACACCGAG TGGCGTGTAA GAGATTGGTC 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC TCTGTTTGCT 
TTGGTACGGA CGGCTCTCAA GATCAAGACT GAAGGCAACC TGGAGCAAGC TAATGAAGAG 
CTCCGCGCTG TGATAAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTACT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAGCGG AAAGAGCAAG GCCTGGTGGG GAAATACCCT 
GCGAAGAACA CCACGATCGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AACCAGAAGA CTCCAAACCA 
GAAGAGGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACTATGT CAATCATGTT 
AATAGTGATA GGAGAGAGTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATTCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGTGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGT TCCCACCTCC 
ACAAATGCCA ATCTCAATAA CGCCAATATG TCCAAAGCTG CCCACGGAAA GCGGCCCAGC 
ATTGGGGACC TTGAGCATGT GTCTGAAAAC GGCCATTACT CCTACAAGCA CGACCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGGTCT GAGTCAGGAG ACGAGCAGCT TCCAACCATT 
TGCCGTGAAG ACCCTGAGAT ACACGGCTAC TTCCGAGACC CCCGCTGCTT CGGGGAGCAG 
GAATATTTCA GTAGCGAGGA GTGCTGTGAG GATGACAGTT CTCCCACCTG GAGCAGGCAA 
AACTACAGCT ATTACAACAG GTACCCAGGC AGCTCCATGG ACTTTGAGAG GCCCCGAGGC 
TACCATCACC CCCAAGGTTT CTTAGAAGAT GACGACTCTC CCATTGGCTA TGACTCACGG 
AGATCTCCAA GAAGACGCCT TCTACCTCCT ACCCCACCAT CCCATCGGAG GTCCTCCTTC 
AACTTTGAGT GTCTGCGCAG GCAAAACAGC CAAGATGACG TCCTTCCGTC GCCTGCCCTG 
CCTCACCGCG CTGCCCTGCC CCTGCACCTT ATGCAGCAGC AGATCATGGC CGTTGCAGGC 
CTAGATTCCA GTAAAGCCCA GAAGTACTCG CCGAGCCACT CTACCCGGTC ATGGGCCACC 
CCTCCAGCAA CCCCTCCGTA CCGAGACTGG ACCCCATGCT ACACCCCTTT GATCCAGGTG 
GATCGCTCAG AGTCTATGGA CCAGGTCAAT GGCAGCCTGC CGTCGCTACA CCGCAGTTCC 
TGGTATACAG ATGAACCCGA CATCTCCTAT AGGACTTTCA CACCGGCCAG CCTGACCGTC 
CCCAGCAGCT TCCGGAACAA AAACAGCGAC AAGCAGAGGA GTGCAGACAG CCTGGTGGAA 
GCAGTCCTGA TATCCGAAGG CTTGGGACGC TACGCAAGGG ACCCAAAATT TGTCTCAGCA 
ACAAAACATG AGATCGCCGA TGCCTGTGAC CTAACCATAG ACGAGATGGA GAGCGCGGCC 
AGCACCCTGC TCAATGGAAG CGTGTGTCCT CGAGCCAACG GGGACATGGG CCCCATCTCA 
CACCGCCAGG ACTATGAGCT CCAGGACTTT GGTCCTGGCT ACAGTGACGA GGAACCAGAC 
CCTGGGCGAG AGGAAGAGGA CCTGGCAGAT GAAATGATTT GCATCACCAC CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455546.1
CDS	418..7014
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSDSENIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017600057.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600057.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GACAGTGAGA ATATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAGCT TCTCAGCAGA 
GGGGAAGGCA TCCGGACTCT GCTATGGACC TTCATTAAGT CCTTCCAGGC ACTCCCATAT 
GTCGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCCG TCATTGGCAT GCAGATGTTT 
GGGAAGGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACGTTT 
CCCCAGGCAG TGCTGCTGCT CTTCAGGTGT GCAACAGGGG AGGCCTGGCA GGAGATCATG 
CTCGCCTGCC TCCCTGGGAA GCTGTGTGAC CCGGACTCAG ATTACAACCC AGGAGAGGAA 
TATACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCG 
TTCCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACGAGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCTGAATAT 
GACCCCGAGG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTTTGCT CCGACGTATC 
CAGCCTCCCC TGGGATTTGG AAAGTTGTGT CCACACCGAG TGGCGTGTAA GAGATTGGTC 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC TCTGTTTGCT 
TTGGTACGGA CGGCTCTCAA GATCAAGACT GAAGGCAACC TGGAGCAAGC TAATGAAGAG 
CTCCGCGCTG TGATAAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTACT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAGCGG AAAGAGCAAG GCCTGGTGGG GAAATACCCT 
GCGAAGAACA CCACGATCGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AACCAGAAGA CTCCAAACCA 
GAAGAGGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACTATGT CAATCATGTT 
AATAGTGATA GGAGAGAGTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATTCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGTGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGT TCCCACCTCC 
ACAAATGCCA ATCTCAATAA CGCCAATATG TCCAAAGCTG CCCACGGAAA GCGGCCCAGC 
ATTGGGGACC TTGAGCATGT GTCTGAAAAC GGCCATTACT CCTACAAGCA CGACCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGGTCT GAGTCAGGAG ACGAGCAGCT TCCAACCATT 
TGCCGTGAAG ACCCTGAGAT ACACGGCTAC TTCCGAGACC CCCGCTGCTT CGGGGAGCAG 
GAATATTTCA GTAGCGAGGA GTGCTGTGAG GATGACAGTT CTCCCACCTG GAGCAGGCAA 
AACTACAGCT ATTACAACAG GTACCCAGGC AGCTCCATGG ACTTTGAGAG GCCCCGAGGC 
TACCATCACC CCCAAGGTTT CTTAGAAGAT GACGACTCTC CCATTGGCTA TGACTCACGG 
AGATCTCCAA GAAGACGCCT TCTACCTCCT ACCCCACCAT CCCATCGGAG GTCCTCCTTC 
AACTTTGAGT GTCTGCGCAG GCAAAACAGC CAAGATGACG TCCTTCCGTC GCCTGCCCTG 
CCTCACCGCG CTGCCCTGCC CCTGCACCTT ATGCAGCAGC AGATCATGGC CGTTGCAGGC 
CTAGATTCCA GTAAAGCCCA GAAGTACTCG CCGAGCCACT CTACCCGGTC ATGGGCCACC 
CCTCCAGCAA CCCCTCCGTA CCGAGACTGG ACCCCATGCT ACACCCCTTT GATCCAGGTG 
GATCGCTCAG AGTCTATGGA CCAGGTCAAT GGCAGCCTGC CGTCGCTACA CCGCAGTTCC 
TGGTATACAG ATGAACCCGA CATCTCCTAT AGGACTTTCA CACCGGCCAG CCTGACCGTC 
CCCAGCAGCT TCCGGAACAA AAACAGCGAC AAGCAGAGGA GTGCAGACAG CCTGGTGGAA 
GCAGTCCTGA TATCCGAAGG CTTGGGACGC TACGCAAGGG ACCCAAAATT TGTCTCAGCA 
ACAAAACATG AGATCGCCGA TGCCTGTGAC CTAACCATAG ACGAGATGGA GAGCGCGGCC 
AGCACCCTGC TCAATGGAAG CGTGTGTCCT CGAGCCAACG GGGACATGGG CCCCATCTCA 
CACCGCCAGG ACTATGAGCT CCAGGACTTT GGTCCTGGCT ACAGTGACGA GGAACCAGAC 
CCTGGGCGAG AGGAAGAGGA CCTGGCAGAT GAAATGATTT GCATCACCAC CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600058.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455547.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X4, mRNA.

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CloneID	ORa58781
Clone ID Related Accession (Same CDS sequence)	XM_017600058.1
Accession Version	XM_017600058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAGCTTCTCA GCAGAGGGGA AGGCATCCGG ACTCTGCTAT GGACCTTCAT TAAGTCCTTC 
CAGGCACTCC CATATGTCGC CCTCCTCATT GCCATGCTCT TCTTCATCTA CGCCGTCATT 
GGCATGCAGA TGTTTGGGAA GGTTGCCATG AGAGATAACA ACCAGATCAA TAGGAACAAC 
AACTTCCAGA CGTTTCCCCA GGCAGTGCTG CTGCTCTTCA GGTGTGCAAC AGGGGAGGCC 
TGGCAGGAGA TCATGCTCGC CTGCCTCCCT GGGAAGCTGT GTGACCCGGA CTCAGATTAC 
AACCCAGGAG AGGAATATAC TTGTGGGAGC AACTTTGCCA TTGTCTACTT CATCAGCTTT 
TACATGCTCT GTGCGTTCCT GATCATCAAC CTCTTCGTGG CTGTCATCAT GGACAATTTT 
GACTATCTGA CGAGGGACTG GTCTATTCTG GGGCCTCACC ACTTGGACGA ATTCAAAAGA 
ATATGGTCTG AATATGACCC CGAGGCAAAG GGAAGGATAA AACACCTTGA TGTGGTCACT 
TTGCTCCGAC GTATCCAGCC TCCCCTGGGA TTTGGAAAGT TGTGTCCACA CCGAGTGGCG 
TGTAAGAGAT TGGTCGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTCAAT 
GCAACTCTGT TTGCTTTGGT ACGGACGGCT CTCAAGATCA AGACTGAAGG CAACCTGGAG 
CAAGCTAATG AAGAGCTCCG CGCTGTGATA AAGAAAATCT GGAAGAAGAC AAGCATGAAG 
CTACTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AGCGGAAAGA GCAAGGCCTG 
GTGGGGAAAT ACCCTGCGAA GAACACCACG ATCGCCCTAC AGGCGGGATT AAGGACCCTG 
CATGACATTG GGCCAGAAAT CCGACGGGCT ATATCCTGTG ACTTGCAAGA TGACGAACCA 
GAAGACTCCA AACCAGAAGA GGAAGATGTA TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC 
TATGTCAATC ATGTTAATAG TGATAGGAGA GAGTCCCTTC AGCAGACCAA TACCACCCAC 
CGTCCCCTGC ATGTCCAAAG GCCTTCAATT CCACCTGCAA GTGATACTGA GAAACCGCTG 
TTTCCTCCAG CAGGAAATTC GGTGTGTCAT AACCATCATA ACCATAATTC CATAGGGAAG 
CAAGTTCCCA CCTCCACAAA TGCCAATCTC AATAACGCCA ATATGTCCAA AGCTGCCCAC 
GGAAAGCGGC CCAGCATTGG GGACCTTGAG CATGTGTCTG AAAACGGCCA TTACTCCTAC 
AAGCACGACC GAGAGCTTCA AAGAAGGTCC AGTATTAAAA GAACCCGCTA TTATGAAACT 
TACATTAGGT CTGAGTCAGG AGACGAGCAG CTTCCAACCA TTTGCCGTGA AGACCCTGAG 
ATACACGGCT ACTTCCGAGA CCCCCGCTGC TTCGGGGAGC AGGAATATTT CAGTAGCGAG 
GAGTGCTGTG AGGATGACAG TTCTCCCACC TGGAGCAGGC AAAACTACAG CTATTACAAC 
AGGTACCCAG GCAGCTCCAT GGACTTTGAG AGGCCCCGAG GCTACCATCA CCCCCAAGGT 
TTCTTAGAAG ATGACGACTC TCCCATTGGC TATGACTCAC GGAGATCTCC AAGAAGACGC 
CTTCTACCTC CTACCCCACC ATCCCATCGG AGGTCCTCCT TCAACTTTGA GTGTCTGCGC 
AGGCAAAACA GCCAAGATGA CGTCCTTCCG TCGCCTGCCC TGCCTCACCG CGCTGCCCTG 
CCCCTGCACC TTATGCAGCA GCAGATCATG GCCGTTGCAG GCCTAGATTC CAGTAAAGCC 
CAGAAGTACT CGCCGAGCCA CTCTACCCGG TCATGGGCCA CCCCTCCAGC AACCCCTCCG 
TACCGAGACT GGACCCCATG CTACACCCCT TTGATCCAGG TGGATCGCTC AGAGTCTATG 
GACCAGGTCA ATGGCAGCCT GCCGTCGCTA CACCGCAGTT CCTGGTATAC AGATGAACCC 
GACATCTCCT ATAGGACTTT CACACCGGCC AGCCTGACCG TCCCCAGCAG CTTCCGGAAC 
AAAAACAGCG ACAAGCAGAG GAGTGCAGAC AGCCTGGTGG AAGCAGTCCT GATATCCGAA 
GGCTTGGGAC GCTACGCAAG GGACCCAAAA TTTGTCTCAG CAACAAAACA TGAGATCGCC 
GATGCCTGTG ACCTAACCAT AGACGAGATG GAGAGCGCGG CCAGCACCCT GCTCAATGGA 
AGCGTGTGTC CTCGAGCCAA CGGGGACATG GGCCCCATCT CACACCGCCA GGACTATGAG 
CTCCAGGACT TTGGTCCTGG CTACAGTGAC GAGGAACCAG ACCCTGGGCG AGAGGAAGAG 
GACCTGGCAG ATGAAATGAT TTGCATCACC ACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455547.1
CDS	417..6995
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017600058.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600058.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAGCTTCTCA GCAGAGGGGA AGGCATCCGG ACTCTGCTAT GGACCTTCAT TAAGTCCTTC 
CAGGCACTCC CATATGTCGC CCTCCTCATT GCCATGCTCT TCTTCATCTA CGCCGTCATT 
GGCATGCAGA TGTTTGGGAA GGTTGCCATG AGAGATAACA ACCAGATCAA TAGGAACAAC 
AACTTCCAGA CGTTTCCCCA GGCAGTGCTG CTGCTCTTCA GGTGTGCAAC AGGGGAGGCC 
TGGCAGGAGA TCATGCTCGC CTGCCTCCCT GGGAAGCTGT GTGACCCGGA CTCAGATTAC 
AACCCAGGAG AGGAATATAC TTGTGGGAGC AACTTTGCCA TTGTCTACTT CATCAGCTTT 
TACATGCTCT GTGCGTTCCT GATCATCAAC CTCTTCGTGG CTGTCATCAT GGACAATTTT 
GACTATCTGA CGAGGGACTG GTCTATTCTG GGGCCTCACC ACTTGGACGA ATTCAAAAGA 
ATATGGTCTG AATATGACCC CGAGGCAAAG GGAAGGATAA AACACCTTGA TGTGGTCACT 
TTGCTCCGAC GTATCCAGCC TCCCCTGGGA TTTGGAAAGT TGTGTCCACA CCGAGTGGCG 
TGTAAGAGAT TGGTCGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTCAAT 
GCAACTCTGT TTGCTTTGGT ACGGACGGCT CTCAAGATCA AGACTGAAGG CAACCTGGAG 
CAAGCTAATG AAGAGCTCCG CGCTGTGATA AAGAAAATCT GGAAGAAGAC AAGCATGAAG 
CTACTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AGCGGAAAGA GCAAGGCCTG 
GTGGGGAAAT ACCCTGCGAA GAACACCACG ATCGCCCTAC AGGCGGGATT AAGGACCCTG 
CATGACATTG GGCCAGAAAT CCGACGGGCT ATATCCTGTG ACTTGCAAGA TGACGAACCA 
GAAGACTCCA AACCAGAAGA GGAAGATGTA TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC 
TATGTCAATC ATGTTAATAG TGATAGGAGA GAGTCCCTTC AGCAGACCAA TACCACCCAC 
CGTCCCCTGC ATGTCCAAAG GCCTTCAATT CCACCTGCAA GTGATACTGA GAAACCGCTG 
TTTCCTCCAG CAGGAAATTC GGTGTGTCAT AACCATCATA ACCATAATTC CATAGGGAAG 
CAAGTTCCCA CCTCCACAAA TGCCAATCTC AATAACGCCA ATATGTCCAA AGCTGCCCAC 
GGAAAGCGGC CCAGCATTGG GGACCTTGAG CATGTGTCTG AAAACGGCCA TTACTCCTAC 
AAGCACGACC GAGAGCTTCA AAGAAGGTCC AGTATTAAAA GAACCCGCTA TTATGAAACT 
TACATTAGGT CTGAGTCAGG AGACGAGCAG CTTCCAACCA TTTGCCGTGA AGACCCTGAG 
ATACACGGCT ACTTCCGAGA CCCCCGCTGC TTCGGGGAGC AGGAATATTT CAGTAGCGAG 
GAGTGCTGTG AGGATGACAG TTCTCCCACC TGGAGCAGGC AAAACTACAG CTATTACAAC 
AGGTACCCAG GCAGCTCCAT GGACTTTGAG AGGCCCCGAG GCTACCATCA CCCCCAAGGT 
TTCTTAGAAG ATGACGACTC TCCCATTGGC TATGACTCAC GGAGATCTCC AAGAAGACGC 
CTTCTACCTC CTACCCCACC ATCCCATCGG AGGTCCTCCT TCAACTTTGA GTGTCTGCGC 
AGGCAAAACA GCCAAGATGA CGTCCTTCCG TCGCCTGCCC TGCCTCACCG CGCTGCCCTG 
CCCCTGCACC TTATGCAGCA GCAGATCATG GCCGTTGCAG GCCTAGATTC CAGTAAAGCC 
CAGAAGTACT CGCCGAGCCA CTCTACCCGG TCATGGGCCA CCCCTCCAGC AACCCCTCCG 
TACCGAGACT GGACCCCATG CTACACCCCT TTGATCCAGG TGGATCGCTC AGAGTCTATG 
GACCAGGTCA ATGGCAGCCT GCCGTCGCTA CACCGCAGTT CCTGGTATAC AGATGAACCC 
GACATCTCCT ATAGGACTTT CACACCGGCC AGCCTGACCG TCCCCAGCAG CTTCCGGAAC 
AAAAACAGCG ACAAGCAGAG GAGTGCAGAC AGCCTGGTGG AAGCAGTCCT GATATCCGAA 
GGCTTGGGAC GCTACGCAAG GGACCCAAAA TTTGTCTCAG CAACAAAACA TGAGATCGCC 
GATGCCTGTG ACCTAACCAT AGACGAGATG GAGAGCGCGG CCAGCACCCT GCTCAATGGA 
AGCGTGTGTC CTCGAGCCAA CGGGGACATG GGCCCCATCT CACACCGCCA GGACTATGAG 
CTCCAGGACT TTGGTCCTGG CTACAGTGAC GAGGAACCAG ACCCTGGGCG AGAGGAAGAG 
GACCTGGCAG ATGAAATGAT TTGCATCACC ACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600059.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455548.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X5, mRNA.

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CloneID	ORa58782
Clone ID Related Accession (Same CDS sequence)	XM_017600059.1
Accession Version	XM_017600059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GACAGTGAGA ATATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAGCT TCTCAGCAGA 
GGGGAAGGCA TCCGGACTCT GCTATGGACC TTCATTAAGT CCTTCCAGGC ACTCCCATAT 
GTCGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCCG TCATTGGCAT GCAGATGTTT 
GGGAAGGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACGTTT 
CCCCAGGCAG TGCTGCTGCT CTTCAGGTGT GCAACAGGGG AGGCCTGGCA GGAGATCATG 
CTCGCCTGCC TCCCTGGGAA GCTGTGTGAC CCGGACTCAG ATTACAACCC AGGAGAGGAA 
TATACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCG 
TTCCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACGAGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCTGAATAT 
GACCCCGAGG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTTTGCT CCGACGTATC 
CAGCCTCCCC TGGGATTTGG AAAGTTGTGT CCACACCGAG TGGCGTGTAA GAGATTGGTC 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC TCTGTTTGCT 
TTGGTACGGA CGGCTCTCAA GATCAAGACT GAAGGCAACC TGGAGCAAGC TAATGAAGAG 
CTCCGCGCTG TGATAAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTACT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAGCGG AAAGAGCAAG GCCTGGTGGG GAAATACCCT 
GCGAAGAACA CCACGATCGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AACCAGAAGA CTCCAAACCA 
GAAGAGGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACTATGT CAATCATGTT 
AATAGTGATA GGAGAGAGTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATTCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGTGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGT TCCCACCTCC 
ACAAATGCCA ATCTCAATAA CGCCAATATG TCCAAAGCTG CCCACGGAAA GCGGCCCAGC 
ATTGGGGACC TTGAGCATGT GTCTGAAAAC GGCCATTACT CCTACAAGCA CGACCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTATG AAACTTACAT TAGGTCTGAG 
TCAGGAGACG AGCAGCTTCC AACCATTTGC CGTGAAGACC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTCGG GGAGCAGGAA TATTTCAGTA GCGAGGAGTG CTGTGAGGAT 
GACAGTTCTC CCACCTGGAG CAGGCAAAAC TACAGCTATT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CATCACCCCC AAGGTTTCTT AGAAGATGAC 
GACTCTCCCA TTGGCTATGA CTCACGGAGA TCTCCAAGAA GACGCCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAACAGCCAA 
GATGACGTCC TTCCGTCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTTATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCGCCG 
AGCCACTCTA CCCGGTCATG GGCCACCCCT CCAGCAACCC CTCCGTACCG AGACTGGACC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAT CGCTCAGAGT CTATGGACCA GGTCAATGGC 
AGCCTGCCGT CGCTACACCG CAGTTCCTGG TATACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACAC CGGCCAGCCT GACCGTCCCC AGCAGCTTCC GGAACAAAAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAAGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCAAGGGACC CAAAATTTGT CTCAGCAACA AAACATGAGA TCGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAGAG CGCGGCCAGC ACCCTGCTCA ATGGAAGCGT GTGTCCTCGA 
GCCAACGGGG ACATGGGCCC CATCTCACAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA ACCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAA 
ATGATTTGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455548.1
CDS	417..6980
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSDSENIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017600059.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600059.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GACAGTGAGA ATATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAGCT TCTCAGCAGA 
GGGGAAGGCA TCCGGACTCT GCTATGGACC TTCATTAAGT CCTTCCAGGC ACTCCCATAT 
GTCGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCCG TCATTGGCAT GCAGATGTTT 
GGGAAGGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACGTTT 
CCCCAGGCAG TGCTGCTGCT CTTCAGGTGT GCAACAGGGG AGGCCTGGCA GGAGATCATG 
CTCGCCTGCC TCCCTGGGAA GCTGTGTGAC CCGGACTCAG ATTACAACCC AGGAGAGGAA 
TATACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCG 
TTCCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACGAGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCTGAATAT 
GACCCCGAGG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTTTGCT CCGACGTATC 
CAGCCTCCCC TGGGATTTGG AAAGTTGTGT CCACACCGAG TGGCGTGTAA GAGATTGGTC 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC TCTGTTTGCT 
TTGGTACGGA CGGCTCTCAA GATCAAGACT GAAGGCAACC TGGAGCAAGC TAATGAAGAG 
CTCCGCGCTG TGATAAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTACT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAGCGG AAAGAGCAAG GCCTGGTGGG GAAATACCCT 
GCGAAGAACA CCACGATCGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AACCAGAAGA CTCCAAACCA 
GAAGAGGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACTATGT CAATCATGTT 
AATAGTGATA GGAGAGAGTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATTCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGTGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGT TCCCACCTCC 
ACAAATGCCA ATCTCAATAA CGCCAATATG TCCAAAGCTG CCCACGGAAA GCGGCCCAGC 
ATTGGGGACC TTGAGCATGT GTCTGAAAAC GGCCATTACT CCTACAAGCA CGACCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTATG AAACTTACAT TAGGTCTGAG 
TCAGGAGACG AGCAGCTTCC AACCATTTGC CGTGAAGACC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTCGG GGAGCAGGAA TATTTCAGTA GCGAGGAGTG CTGTGAGGAT 
GACAGTTCTC CCACCTGGAG CAGGCAAAAC TACAGCTATT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CATCACCCCC AAGGTTTCTT AGAAGATGAC 
GACTCTCCCA TTGGCTATGA CTCACGGAGA TCTCCAAGAA GACGCCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAACAGCCAA 
GATGACGTCC TTCCGTCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTTATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCGCCG 
AGCCACTCTA CCCGGTCATG GGCCACCCCT CCAGCAACCC CTCCGTACCG AGACTGGACC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAT CGCTCAGAGT CTATGGACCA GGTCAATGGC 
AGCCTGCCGT CGCTACACCG CAGTTCCTGG TATACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACAC CGGCCAGCCT GACCGTCCCC AGCAGCTTCC GGAACAAAAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAAGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCAAGGGACC CAAAATTTGT CTCAGCAACA AAACATGAGA TCGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAGAG CGCGGCCAGC ACCCTGCTCA ATGGAAGCGT GTGTCCTCGA 
GCCAACGGGG ACATGGGCCC CATCTCACAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA ACCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAA 
ATGATTTGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600060.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455549.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X6, mRNA.

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CloneID	ORa58783
Clone ID Related Accession (Same CDS sequence)	XM_017600060.1
Accession Version	XM_017600060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTGCTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GACAGTGAGA ATATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAGCT TCTCAGCAGA 
GGGGAAGGCA TCCGGACTCT GCTATGGACC TTCATTAAGT CCTTCCAGGC ACTCCCATAT 
GTCGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCCG TCATTGGCAT GCAGATGTTT 
GGGAAGGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACGTTT 
CCCCAGGCAG TGCTGCTGCT CTTCAGGTGT GCAACAGGGG AGGCCTGGCA GGAGATCATG 
CTCGCCTGCC TCCCTGGGAA GCTGTGTGAC CCGGACTCAG ATTACAACCC AGGAGAGGAA 
TATACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCG 
TTCCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACGAGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCTGAATAT 
GACCCCGAGG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTTTGCT CCGACGTATC 
CAGCCTCCCC TGGGATTTGG AAAGTTGTGT CCACACCGAG TGGCGTGTAA GAGATTGGTC 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC TCTGTTTGCT 
TTGGTACGGA CGGCTCTCAA GATCAAGACT GAAGGCAACC TGGAGCAAGC TAATGAAGAG 
CTCCGCGCTG TGATAAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTACT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAGCGG AAAGAGCAAG GCCTGGTGGG GAAATACCCT 
GCGAAGAACA CCACGATCGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AACCAGAAGA CTCCAAACCA 
GAAGAGGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACTATGT CAATCATGTT 
AATAGTGATA GGAGAGAGTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATTCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGTGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGT TCCCACCTCC 
ACAAATGCCA ATCTCAATAA CGCCAATATG TCCAAAGCTG CCCACGGAAA GCGGCCCAGC 
ATTGGGGACC TTGAGCATGT GTCTGAAAAC GGCCATTACT CCTACAAGCA CGACCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTATG AAACTTACAT TAGGTCTGAG 
TCAGGAGACG AGCAGCTTCC AACCATTTGC CGTGAAGACC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTCGG GGAGCAGGAA TATTTCAGTA GCGAGGAGTG CTGTGAGGAT 
GACAGTTCTC CCACCTGGAG CAGGCAAAAC TACAGCTATT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CATCACCCCC AAGGTTTCTT AGAAGATGAC 
GACTCTCCCA TTGGCTATGA CTCACGGAGA TCTCCAAGAA GACGCCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAACAGCCAA 
GATGACGTCC TTCCGTCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTTATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCGCCG 
AGCCACTCTA CCCGGTCATG GGCCACCCCT CCAGCAACCC CTCCGTACCG AGACTGGACC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAT CGCTCAGAGT CTATGGACCA GGTCAATGGC 
AGCCTGCCGT CGCTACACCG CAGTTCCTGG TATACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACAC CGGCCAGCCT GACCGTCCCC AGCAGCTTCC GGAACAAAAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAAGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCAAGGGACC CAAAATTTGT CTCAGCAACA AAACATGAGA TCGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAGAG CGCGGCCAGC ACCCTGCTCA ATGGAAGCGT GTGTCCTCGA 
GCCAACGGGG ACATGGGCCC CATCTCACAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA ACCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAA 
ATGATTTGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455549.1
CDS	417..6980
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSDSENIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017600060.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600060.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTGCTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GACAGTGAGA ATATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAGCT TCTCAGCAGA 
GGGGAAGGCA TCCGGACTCT GCTATGGACC TTCATTAAGT CCTTCCAGGC ACTCCCATAT 
GTCGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCCG TCATTGGCAT GCAGATGTTT 
GGGAAGGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACGTTT 
CCCCAGGCAG TGCTGCTGCT CTTCAGGTGT GCAACAGGGG AGGCCTGGCA GGAGATCATG 
CTCGCCTGCC TCCCTGGGAA GCTGTGTGAC CCGGACTCAG ATTACAACCC AGGAGAGGAA 
TATACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCG 
TTCCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACGAGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCTGAATAT 
GACCCCGAGG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTTTGCT CCGACGTATC 
CAGCCTCCCC TGGGATTTGG AAAGTTGTGT CCACACCGAG TGGCGTGTAA GAGATTGGTC 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC TCTGTTTGCT 
TTGGTACGGA CGGCTCTCAA GATCAAGACT GAAGGCAACC TGGAGCAAGC TAATGAAGAG 
CTCCGCGCTG TGATAAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTACT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAGCGG AAAGAGCAAG GCCTGGTGGG GAAATACCCT 
GCGAAGAACA CCACGATCGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AACCAGAAGA CTCCAAACCA 
GAAGAGGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACTATGT CAATCATGTT 
AATAGTGATA GGAGAGAGTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATTCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGTGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGT TCCCACCTCC 
ACAAATGCCA ATCTCAATAA CGCCAATATG TCCAAAGCTG CCCACGGAAA GCGGCCCAGC 
ATTGGGGACC TTGAGCATGT GTCTGAAAAC GGCCATTACT CCTACAAGCA CGACCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTATG AAACTTACAT TAGGTCTGAG 
TCAGGAGACG AGCAGCTTCC AACCATTTGC CGTGAAGACC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTCGG GGAGCAGGAA TATTTCAGTA GCGAGGAGTG CTGTGAGGAT 
GACAGTTCTC CCACCTGGAG CAGGCAAAAC TACAGCTATT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CATCACCCCC AAGGTTTCTT AGAAGATGAC 
GACTCTCCCA TTGGCTATGA CTCACGGAGA TCTCCAAGAA GACGCCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAACAGCCAA 
GATGACGTCC TTCCGTCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTTATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCGCCG 
AGCCACTCTA CCCGGTCATG GGCCACCCCT CCAGCAACCC CTCCGTACCG AGACTGGACC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAT CGCTCAGAGT CTATGGACCA GGTCAATGGC 
AGCCTGCCGT CGCTACACCG CAGTTCCTGG TATACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACAC CGGCCAGCCT GACCGTCCCC AGCAGCTTCC GGAACAAAAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAAGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCAAGGGACC CAAAATTTGT CTCAGCAACA AAACATGAGA TCGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAGAG CGCGGCCAGC ACCCTGCTCA ATGGAAGCGT GTGTCCTCGA 
GCCAACGGGG ACATGGGCCC CATCTCACAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA ACCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAA 
ATGATTTGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600061.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455550.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X8, mRNA.

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CloneID	ORa58784
Clone ID Related Accession (Same CDS sequence)	XM_017600061.1
Accession Version	XM_017600061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAT ACTTTTGATG CCTTAATTGT TGTCGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGACA GTGAGAATAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAGCTTCTC AGCAGAGGGG AAGGCATCCG GACTCTGCTA 
TGGACCTTCA TTAAGTCCTT CCAGGCACTC CCATATGTCG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCCGTCAT TGGCATGCAG ATGTTTGGGA AGGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACGTTTCCCC AGGCAGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGGGAGGC CTGGCAGGAG ATCATGCTCG CCTGCCTCCC TGGGAAGCTG 
TGTGACCCGG ACTCAGATTA CAACCCAGGA GAGGAATATA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCGTTCC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACGAGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCT GAATATGACC CCGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TTTGCTCCGA CGTATCCAGC CTCCCCTGGG ATTTGGAAAG 
TTGTGTCCAC ACCGAGTGGC GTGTAAGAGA TTGGTCGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACTCTG TTTGCTTTGG TACGGACGGC TCTCAAGATC 
AAGACTGAAG GCAACCTGGA GCAAGCTAAT GAAGAGCTCC GCGCTGTGAT AAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAGCGGAAAG AGCAAGGCCT GGTGGGGAAA TACCCTGCGA AGAACACCAC GATCGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAACC AGAAGACTCC AAACCAGAAG AGGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CTATGTCAAT CATGTTAATA GTGATAGGAG AGAGTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT TCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGTGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGTTCCC ACCTCCACAA ATGCCAATCT CAATAACGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCGG CCCAGCATTG GGGACCTTGA GCATGTGTCT 
GAAAACGGCC ATTACTCCTA CAAGCACGAC CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTATGAAAC TTACATTAGG TCTGAGTCAG GAGACGAGCA GCTTCCAACC 
ATTTGCCGTG AAGACCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTCGGGGAG 
CAGGAATATT TCAGTAGCGA GGAGTGCTGT GAGGATGACA GTTCTCCCAC CTGGAGCAGG 
CAAAACTACA GCTATTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCATC ACCCCCAAGG TTTCTTAGAA GATGACGACT CTCCCATTGG CTATGACTCA 
CGGAGATCTC CAAGAAGACG CCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAAC AGCCAAGATG ACGTCCTTCC GTCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTTATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCGCCGAGCC ACTCTACCCG GTCATGGGCC 
ACCCCTCCAG CAACCCCTCC GTACCGAGAC TGGACCCCAT GCTACACCCC TTTGATCCAG 
GTGGATCGCT CAGAGTCTAT GGACCAGGTC AATGGCAGCC TGCCGTCGCT ACACCGCAGT 
TCCTGGTATA CAGATGAACC CGACATCTCC TATAGGACTT TCACACCGGC CAGCCTGACC 
GTCCCCAGCA GCTTCCGGAA CAAAAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAAGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCAA GGGACCCAAA ATTTGTCTCA 
GCAACAAAAC ATGAGATCGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAGAGCGCG 
GCCAGCACCC TGCTCAATGG AAGCGTGTGT CCTCGAGCCA ACGGGGACAT GGGCCCCATC 
TCACACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAACCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAAATGA TTTGCATCAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455550.1
CDS	417..6956
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPSDSENIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017600061.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600061.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAT ACTTTTGATG CCTTAATTGT TGTCGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGACA GTGAGAATAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAGCTTCTC AGCAGAGGGG AAGGCATCCG GACTCTGCTA 
TGGACCTTCA TTAAGTCCTT CCAGGCACTC CCATATGTCG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCCGTCAT TGGCATGCAG ATGTTTGGGA AGGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACGTTTCCCC AGGCAGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGGGAGGC CTGGCAGGAG ATCATGCTCG CCTGCCTCCC TGGGAAGCTG 
TGTGACCCGG ACTCAGATTA CAACCCAGGA GAGGAATATA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCGTTCC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACGAGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCT GAATATGACC CCGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TTTGCTCCGA CGTATCCAGC CTCCCCTGGG ATTTGGAAAG 
TTGTGTCCAC ACCGAGTGGC GTGTAAGAGA TTGGTCGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACTCTG TTTGCTTTGG TACGGACGGC TCTCAAGATC 
AAGACTGAAG GCAACCTGGA GCAAGCTAAT GAAGAGCTCC GCGCTGTGAT AAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAGCGGAAAG AGCAAGGCCT GGTGGGGAAA TACCCTGCGA AGAACACCAC GATCGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAACC AGAAGACTCC AAACCAGAAG AGGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CTATGTCAAT CATGTTAATA GTGATAGGAG AGAGTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT TCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGTGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGTTCCC ACCTCCACAA ATGCCAATCT CAATAACGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCGG CCCAGCATTG GGGACCTTGA GCATGTGTCT 
GAAAACGGCC ATTACTCCTA CAAGCACGAC CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTATGAAAC TTACATTAGG TCTGAGTCAG GAGACGAGCA GCTTCCAACC 
ATTTGCCGTG AAGACCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTCGGGGAG 
CAGGAATATT TCAGTAGCGA GGAGTGCTGT GAGGATGACA GTTCTCCCAC CTGGAGCAGG 
CAAAACTACA GCTATTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCATC ACCCCCAAGG TTTCTTAGAA GATGACGACT CTCCCATTGG CTATGACTCA 
CGGAGATCTC CAAGAAGACG CCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAAC AGCCAAGATG ACGTCCTTCC GTCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTTATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCGCCGAGCC ACTCTACCCG GTCATGGGCC 
ACCCCTCCAG CAACCCCTCC GTACCGAGAC TGGACCCCAT GCTACACCCC TTTGATCCAG 
GTGGATCGCT CAGAGTCTAT GGACCAGGTC AATGGCAGCC TGCCGTCGCT ACACCGCAGT 
TCCTGGTATA CAGATGAACC CGACATCTCC TATAGGACTT TCACACCGGC CAGCCTGACC 
GTCCCCAGCA GCTTCCGGAA CAAAAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAAGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCAA GGGACCCAAA ATTTGTCTCA 
GCAACAAAAC ATGAGATCGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAGAGCGCG 
GCCAGCACCC TGCTCAATGG AAGCGTGTGT CCTCGAGCCA ACGGGGACAT GGGCCCCATC 
TCACACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAACCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAAATGA TTTGCATCAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600062.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455551.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X9, mRNA.

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CloneID	ORa58785
Clone ID Related Accession (Same CDS sequence)	XM_017600062.1
Accession Version	XM_017600062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTGCTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAT ACTTTTGATG CCTTAATTGT TGTCGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGACA GTGAGAATAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAGCTTCTC AGCAGAGGGG AAGGCATCCG GACTCTGCTA 
TGGACCTTCA TTAAGTCCTT CCAGGCACTC CCATATGTCG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCCGTCAT TGGCATGCAG ATGTTTGGGA AGGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACGTTTCCCC AGGCAGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGGGAGGC CTGGCAGGAG ATCATGCTCG CCTGCCTCCC TGGGAAGCTG 
TGTGACCCGG ACTCAGATTA CAACCCAGGA GAGGAATATA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCGTTCC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACGAGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCT GAATATGACC CCGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TTTGCTCCGA CGTATCCAGC CTCCCCTGGG ATTTGGAAAG 
TTGTGTCCAC ACCGAGTGGC GTGTAAGAGA TTGGTCGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACTCTG TTTGCTTTGG TACGGACGGC TCTCAAGATC 
AAGACTGAAG GCAACCTGGA GCAAGCTAAT GAAGAGCTCC GCGCTGTGAT AAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAGCGGAAAG AGCAAGGCCT GGTGGGGAAA TACCCTGCGA AGAACACCAC GATCGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAACC AGAAGACTCC AAACCAGAAG AGGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CTATGTCAAT CATGTTAATA GTGATAGGAG AGAGTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT TCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGTGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGTTCCC ACCTCCACAA ATGCCAATCT CAATAACGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCGG CCCAGCATTG GGGACCTTGA GCATGTGTCT 
GAAAACGGCC ATTACTCCTA CAAGCACGAC CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTATGAAAC TTACATTAGG TCTGAGTCAG GAGACGAGCA GCTTCCAACC 
ATTTGCCGTG AAGACCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTCGGGGAG 
CAGGAATATT TCAGTAGCGA GGAGTGCTGT GAGGATGACA GTTCTCCCAC CTGGAGCAGG 
CAAAACTACA GCTATTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCATC ACCCCCAAGG TTTCTTAGAA GATGACGACT CTCCCATTGG CTATGACTCA 
CGGAGATCTC CAAGAAGACG CCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAAC AGCCAAGATG ACGTCCTTCC GTCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTTATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCGCCGAGCC ACTCTACCCG GTCATGGGCC 
ACCCCTCCAG CAACCCCTCC GTACCGAGAC TGGACCCCAT GCTACACCCC TTTGATCCAG 
GTGGATCGCT CAGAGTCTAT GGACCAGGTC AATGGCAGCC TGCCGTCGCT ACACCGCAGT 
TCCTGGTATA CAGATGAACC CGACATCTCC TATAGGACTT TCACACCGGC CAGCCTGACC 
GTCCCCAGCA GCTTCCGGAA CAAAAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAAGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCAA GGGACCCAAA ATTTGTCTCA 
GCAACAAAAC ATGAGATCGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAGAGCGCG 
GCCAGCACCC TGCTCAATGG AAGCGTGTGT CCTCGAGCCA ACGGGGACAT GGGCCCCATC 
TCACACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAACCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAAATGA TTTGCATCAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455551.1
CDS	418..6957
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPSDSENIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017600062.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600062.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTGCTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAT ACTTTTGATG CCTTAATTGT TGTCGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGACA GTGAGAATAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAGCTTCTC AGCAGAGGGG AAGGCATCCG GACTCTGCTA 
TGGACCTTCA TTAAGTCCTT CCAGGCACTC CCATATGTCG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCCGTCAT TGGCATGCAG ATGTTTGGGA AGGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACGTTTCCCC AGGCAGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGGGAGGC CTGGCAGGAG ATCATGCTCG CCTGCCTCCC TGGGAAGCTG 
TGTGACCCGG ACTCAGATTA CAACCCAGGA GAGGAATATA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCGTTCC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACGAGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCT GAATATGACC CCGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TTTGCTCCGA CGTATCCAGC CTCCCCTGGG ATTTGGAAAG 
TTGTGTCCAC ACCGAGTGGC GTGTAAGAGA TTGGTCGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACTCTG TTTGCTTTGG TACGGACGGC TCTCAAGATC 
AAGACTGAAG GCAACCTGGA GCAAGCTAAT GAAGAGCTCC GCGCTGTGAT AAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAGCGGAAAG AGCAAGGCCT GGTGGGGAAA TACCCTGCGA AGAACACCAC GATCGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAACC AGAAGACTCC AAACCAGAAG AGGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CTATGTCAAT CATGTTAATA GTGATAGGAG AGAGTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT TCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGTGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGTTCCC ACCTCCACAA ATGCCAATCT CAATAACGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCGG CCCAGCATTG GGGACCTTGA GCATGTGTCT 
GAAAACGGCC ATTACTCCTA CAAGCACGAC CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTATGAAAC TTACATTAGG TCTGAGTCAG GAGACGAGCA GCTTCCAACC 
ATTTGCCGTG AAGACCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTCGGGGAG 
CAGGAATATT TCAGTAGCGA GGAGTGCTGT GAGGATGACA GTTCTCCCAC CTGGAGCAGG 
CAAAACTACA GCTATTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCATC ACCCCCAAGG TTTCTTAGAA GATGACGACT CTCCCATTGG CTATGACTCA 
CGGAGATCTC CAAGAAGACG CCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAAC AGCCAAGATG ACGTCCTTCC GTCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTTATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCGCCGAGCC ACTCTACCCG GTCATGGGCC 
ACCCCTCCAG CAACCCCTCC GTACCGAGAC TGGACCCCAT GCTACACCCC TTTGATCCAG 
GTGGATCGCT CAGAGTCTAT GGACCAGGTC AATGGCAGCC TGCCGTCGCT ACACCGCAGT 
TCCTGGTATA CAGATGAACC CGACATCTCC TATAGGACTT TCACACCGGC CAGCCTGACC 
GTCCCCAGCA GCTTCCGGAA CAAAAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAAGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCAA GGGACCCAAA ATTTGTCTCA 
GCAACAAAAC ATGAGATCGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAGAGCGCG 
GCCAGCACCC TGCTCAATGG AAGCGTGTGT CCTCGAGCCA ACGGGGACAT GGGCCCCATC 
TCACACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAACCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAAATGA TTTGCATCAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600063.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455552.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X10, mRNA.

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CloneID	ORa58786
Clone ID Related Accession (Same CDS sequence)	XM_017600063.1
Accession Version	XM_017600063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAGC TTCTCAGCAG AGGGGAAGGC 
ATCCGGACTC TGCTATGGAC CTTCATTAAG TCCTTCCAGG CACTCCCATA TGTCGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCC GTCATTGGCA TGCAGATGTT TGGGAAGGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACGTT TCCCCAGGCA 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGG GAGGCCTGGC AGGAGATCAT GCTCGCCTGC 
CTCCCTGGGA AGCTGTGTGA CCCGGACTCA GATTACAACC CAGGAGAGGA ATATACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC GTTCCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACGAG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCTGAATA TGACCCCGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTTTGC TCCGACGTAT CCAGCCTCCC 
CTGGGATTTG GAAAGTTGTG TCCACACCGA GTGGCGTGTA AGAGATTGGT CGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CTCTGTTTGC TTTGGTACGG 
ACGGCTCTCA AGATCAAGAC TGAAGGCAAC CTGGAGCAAG CTAATGAAGA GCTCCGCGCT 
GTGATAAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAGCG GAAAGAGCAA GGCCTGGTGG GGAAATACCC TGCGAAGAAC 
ACCACGATCG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAACCAGAAG ACTCCAAACC AGAAGAGGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACTATG TCAATCATGT TAATAGTGAT 
AGGAGAGAGT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATTCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGTG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG TTCCCACCTC CACAAATGCC 
AATCTCAATA ACGCCAATAT GTCCAAAGCT GCCCACGGAA AGCGGCCCAG CATTGGGGAC 
CTTGAGCATG TGTCTGAAAA CGGCCATTAC TCCTACAAGC ACGACCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAT GAAACTTACA TTAGGTCTGA GTCAGGAGAC 
GAGCAGCTTC CAACCATTTG CCGTGAAGAC CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTCG GGGAGCAGGA ATATTTCAGT AGCGAGGAGT GCTGTGAGGA TGACAGTTCT 
CCCACCTGGA GCAGGCAAAA CTACAGCTAT TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCATCACCCC CAAGGTTTCT TAGAAGATGA CGACTCTCCC 
ATTGGCTATG ACTCACGGAG ATCTCCAAGA AGACGCCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAACAGCCA AGATGACGTC 
CTTCCGTCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTTAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCGCC GAGCCACTCT 
ACCCGGTCAT GGGCCACCCC TCCAGCAACC CCTCCGTACC GAGACTGGAC CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA TCGCTCAGAG TCTATGGACC AGGTCAATGG CAGCCTGCCG 
TCGCTACACC GCAGTTCCTG GTATACAGAT GAACCCGACA TCTCCTATAG GACTTTCACA 
CCGGCCAGCC TGACCGTCCC CAGCAGCTTC CGGAACAAAA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAAGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCAAGGGAC 
CCAAAATTTG TCTCAGCAAC AAAACATGAG ATCGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAGA GCGCGGCCAG CACCCTGCTC AATGGAAGCG TGTGTCCTCG AGCCAACGGG 
GACATGGGCC CCATCTCACA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AACCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA AATGATTTGC 
ATCACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455552.1
CDS	417..6911
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017600063.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600063.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAGC TTCTCAGCAG AGGGGAAGGC 
ATCCGGACTC TGCTATGGAC CTTCATTAAG TCCTTCCAGG CACTCCCATA TGTCGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCC GTCATTGGCA TGCAGATGTT TGGGAAGGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACGTT TCCCCAGGCA 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGG GAGGCCTGGC AGGAGATCAT GCTCGCCTGC 
CTCCCTGGGA AGCTGTGTGA CCCGGACTCA GATTACAACC CAGGAGAGGA ATATACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC GTTCCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACGAG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCTGAATA TGACCCCGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTTTGC TCCGACGTAT CCAGCCTCCC 
CTGGGATTTG GAAAGTTGTG TCCACACCGA GTGGCGTGTA AGAGATTGGT CGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CTCTGTTTGC TTTGGTACGG 
ACGGCTCTCA AGATCAAGAC TGAAGGCAAC CTGGAGCAAG CTAATGAAGA GCTCCGCGCT 
GTGATAAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAGCG GAAAGAGCAA GGCCTGGTGG GGAAATACCC TGCGAAGAAC 
ACCACGATCG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAACCAGAAG ACTCCAAACC AGAAGAGGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACTATG TCAATCATGT TAATAGTGAT 
AGGAGAGAGT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATTCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGTG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG TTCCCACCTC CACAAATGCC 
AATCTCAATA ACGCCAATAT GTCCAAAGCT GCCCACGGAA AGCGGCCCAG CATTGGGGAC 
CTTGAGCATG TGTCTGAAAA CGGCCATTAC TCCTACAAGC ACGACCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAT GAAACTTACA TTAGGTCTGA GTCAGGAGAC 
GAGCAGCTTC CAACCATTTG CCGTGAAGAC CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTCG GGGAGCAGGA ATATTTCAGT AGCGAGGAGT GCTGTGAGGA TGACAGTTCT 
CCCACCTGGA GCAGGCAAAA CTACAGCTAT TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCATCACCCC CAAGGTTTCT TAGAAGATGA CGACTCTCCC 
ATTGGCTATG ACTCACGGAG ATCTCCAAGA AGACGCCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAACAGCCA AGATGACGTC 
CTTCCGTCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTTAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCGCC GAGCCACTCT 
ACCCGGTCAT GGGCCACCCC TCCAGCAACC CCTCCGTACC GAGACTGGAC CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA TCGCTCAGAG TCTATGGACC AGGTCAATGG CAGCCTGCCG 
TCGCTACACC GCAGTTCCTG GTATACAGAT GAACCCGACA TCTCCTATAG GACTTTCACA 
CCGGCCAGCC TGACCGTCCC CAGCAGCTTC CGGAACAAAA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAAGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCAAGGGAC 
CCAAAATTTG TCTCAGCAAC AAAACATGAG ATCGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAGA GCGCGGCCAG CACCCTGCTC AATGGAAGCG TGTGTCCTCG AGCCAACGGG 
GACATGGGCC CCATCTCACA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AACCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA AATGATTTGC 
ATCACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600064.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455553.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X11, mRNA.

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CloneID	ORa58787
Clone ID Related Accession (Same CDS sequence)	XM_017600064.1
Accession Version	XM_017600064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAT ACTTTTGATG CCTTAATTGT TGTCGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAGC TTCTCAGCAG AGGGGAAGGC 
ATCCGGACTC TGCTATGGAC CTTCATTAAG TCCTTCCAGG CACTCCCATA TGTCGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCC GTCATTGGCA TGCAGATGTT TGGGAAGGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACGTT TCCCCAGGCA 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGG GAGGCCTGGC AGGAGATCAT GCTCGCCTGC 
CTCCCTGGGA AGCTGTGTGA CCCGGACTCA GATTACAACC CAGGAGAGGA ATATACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC GTTCCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACGAG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCTGAATA TGACCCCGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTTTGC TCCGACGTAT CCAGCCTCCC 
CTGGGATTTG GAAAGTTGTG TCCACACCGA GTGGCGTGTA AGAGATTGGT CGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CTCTGTTTGC TTTGGTACGG 
ACGGCTCTCA AGATCAAGAC TGAAGGCAAC CTGGAGCAAG CTAATGAAGA GCTCCGCGCT 
GTGATAAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAGCG GAAAGAGCAA GGCCTGGTGG GGAAATACCC TGCGAAGAAC 
ACCACGATCG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAACCAGAAG ACTCCAAACC AGAAGAGGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACTATG TCAATCATGT TAATAGTGAT 
AGGAGAGAGT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATTCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGTG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG TTCCCACCTC CACAAATGCC 
AATCTCAATA ACGCCAATAT GTCCAAAGCT GCCCACGGAA AGCGGCCCAG CATTGGGGAC 
CTTGAGCATG TGTCTGAAAA CGGCCATTAC TCCTACAAGC ACGACCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAT GAAACTTACA TTAGGTCTGA GTCAGGAGAC 
GAGCAGCTTC CAACCATTTG CCGTGAAGAC CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTCG GGGAGCAGGA ATATTTCAGT AGCGAGGAGT GCTGTGAGGA TGACAGTTCT 
CCCACCTGGA GCAGGCAAAA CTACAGCTAT TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCATCACCCC CAAGGTTTCT TAGAAGATGA CGACTCTCCC 
ATTGGCTATG ACTCACGGAG ATCTCCAAGA AGACGCCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAACAGCCA AGATGACGTC 
CTTCCGTCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTTAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCGCC GAGCCACTCT 
ACCCGGTCAT GGGCCACCCC TCCAGCAACC CCTCCGTACC GAGACTGGAC CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA TCGCTCAGAG TCTATGGACC AGGTCAATGG CAGCCTGCCG 
TCGCTACACC GCAGTTCCTG GTATACAGAT GAACCCGACA TCTCCTATAG GACTTTCACA 
CCGGCCAGCC TGACCGTCCC CAGCAGCTTC CGGAACAAAA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAAGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCAAGGGAC 
CCAAAATTTG TCTCAGCAAC AAAACATGAG ATCGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAGA GCGCGGCCAG CACCCTGCTC AATGGAAGCG TGTGTCCTCG AGCCAACGGG 
GACATGGGCC CCATCTCACA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AACCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA AATGATTTGC 
ATCACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455553.1
CDS	417..6911
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017600064.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600064.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAT ACTTTTGATG CCTTAATTGT TGTCGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAGC TTCTCAGCAG AGGGGAAGGC 
ATCCGGACTC TGCTATGGAC CTTCATTAAG TCCTTCCAGG CACTCCCATA TGTCGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCC GTCATTGGCA TGCAGATGTT TGGGAAGGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACGTT TCCCCAGGCA 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGG GAGGCCTGGC AGGAGATCAT GCTCGCCTGC 
CTCCCTGGGA AGCTGTGTGA CCCGGACTCA GATTACAACC CAGGAGAGGA ATATACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC GTTCCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACGAG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCTGAATA TGACCCCGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTTTGC TCCGACGTAT CCAGCCTCCC 
CTGGGATTTG GAAAGTTGTG TCCACACCGA GTGGCGTGTA AGAGATTGGT CGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CTCTGTTTGC TTTGGTACGG 
ACGGCTCTCA AGATCAAGAC TGAAGGCAAC CTGGAGCAAG CTAATGAAGA GCTCCGCGCT 
GTGATAAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAGCG GAAAGAGCAA GGCCTGGTGG GGAAATACCC TGCGAAGAAC 
ACCACGATCG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAACCAGAAG ACTCCAAACC AGAAGAGGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACTATG TCAATCATGT TAATAGTGAT 
AGGAGAGAGT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATTCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGTG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG TTCCCACCTC CACAAATGCC 
AATCTCAATA ACGCCAATAT GTCCAAAGCT GCCCACGGAA AGCGGCCCAG CATTGGGGAC 
CTTGAGCATG TGTCTGAAAA CGGCCATTAC TCCTACAAGC ACGACCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAT GAAACTTACA TTAGGTCTGA GTCAGGAGAC 
GAGCAGCTTC CAACCATTTG CCGTGAAGAC CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTCG GGGAGCAGGA ATATTTCAGT AGCGAGGAGT GCTGTGAGGA TGACAGTTCT 
CCCACCTGGA GCAGGCAAAA CTACAGCTAT TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCATCACCCC CAAGGTTTCT TAGAAGATGA CGACTCTCCC 
ATTGGCTATG ACTCACGGAG ATCTCCAAGA AGACGCCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAACAGCCA AGATGACGTC 
CTTCCGTCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTTAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCGCC GAGCCACTCT 
ACCCGGTCAT GGGCCACCCC TCCAGCAACC CCTCCGTACC GAGACTGGAC CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA TCGCTCAGAG TCTATGGACC AGGTCAATGG CAGCCTGCCG 
TCGCTACACC GCAGTTCCTG GTATACAGAT GAACCCGACA TCTCCTATAG GACTTTCACA 
CCGGCCAGCC TGACCGTCCC CAGCAGCTTC CGGAACAAAA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAAGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCAAGGGAC 
CCAAAATTTG TCTCAGCAAC AAAACATGAG ATCGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAGA GCGCGGCCAG CACCCTGCTC AATGGAAGCG TGTGTCCTCG AGCCAACGGG 
GACATGGGCC CCATCTCACA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AACCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA AATGATTTGC 
ATCACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600065.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455554.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X14, mRNA.

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CloneID	ORa58788
Clone ID Related Accession (Same CDS sequence)	XM_017600065.1
Accession Version	XM_017600065.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGGTTGG TGAAGCTTCT CAGCAGAGGG GAAGGCATCC GGACTCTGCT ATGGACCTTC 
ATTAAGTCCT TCCAGGCACT CCCATATGTC GCCCTCCTCA TTGCCATGCT CTTCTTCATC 
TACGCCGTCA TTGGCATGCA GATGTTTGGG AAGGTTGCCA TGAGAGATAA CAACCAGATC 
AATAGGAACA ACAACTTCCA GACGTTTCCC CAGGCAGTGC TGCTGCTCTT CAGGTGTGCA 
ACAGGGGAGG CCTGGCAGGA GATCATGCTC GCCTGCCTCC CTGGGAAGCT GTGTGACCCG 
GACTCAGATT ACAACCCAGG AGAGGAATAT ACTTGTGGGA GCAACTTTGC CATTGTCTAC 
TTCATCAGCT TTTACATGCT CTGTGCGTTC CTGATCATCA ACCTCTTCGT GGCTGTCATC 
ATGGACAATT TTGACTATCT GACGAGGGAC TGGTCTATTC TGGGGCCTCA CCACTTGGAC 
GAATTCAAAA GAATATGGTC TGAATATGAC CCCGAGGCAA AGGGAAGGAT AAAACACCTT 
GATGTGGTCA CTTTGCTCCG ACGTATCCAG CCTCCCCTGG GATTTGGAAA GTTGTGTCCA 
CACCGAGTGG CGTGTAAGAG ATTGGTCGCC ATGAACATGC CTCTCAACAG TGATGGGACA 
GTCATGTTCA ATGCAACTCT GTTTGCTTTG GTACGGACGG CTCTCAAGAT CAAGACTGAA 
GGCAACCTGG AGCAAGCTAA TGAAGAGCTC CGCGCTGTGA TAAAGAAAAT CTGGAAGAAG 
ACAAGCATGA AGCTACTTGA CCAAGTTGTC CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAGCGGAAA 
GAGCAAGGCC TGGTGGGGAA ATACCCTGCG AAGAACACCA CGATCGCCCT ACAGGCGGGA 
TTAAGGACCC TGCATGACAT TGGGCCAGAA ATCCGACGGG CTATATCCTG TGACTTGCAA 
GATGACGAAC CAGAAGACTC CAAACCAGAA GAGGAAGATG TATTCAAAAG AAATGGTGCC 
CTGCTTGGAA ACTATGTCAA TCATGTTAAT AGTGATAGGA GAGAGTCCCT TCAGCAGACC 
AATACCACCC ACCGTCCCCT GCATGTCCAA AGGCCTTCAA TTCCACCTGC AAGTGATACT 
GAGAAACCGC TGTTTCCTCC AGCAGGAAAT TCGGTGTGTC ATAACCATCA TAACCATAAT 
TCCATAGGGA AGCAAGTTCC CACCTCCACA AATGCCAATC TCAATAACGC CAATATGTCC 
AAAGCTGCCC ACGGAAAGCG GCCCAGCATT GGGGACCTTG AGCATGTGTC TGAAAACGGC 
CATTACTCCT ACAAGCACGA CCGAGAGCTT CAAAGAAGGT CCAGTATTAA AAGAACCCGC 
TATTATGAAA CTTACATTAG GTCTGAGTCA GGAGACGAGC AGCTTCCAAC CATTTGCCGT 
GAAGACCCTG AGATACACGG CTACTTCCGA GACCCCCGCT GCTTCGGGGA GCAGGAATAT 
TTCAGTAGCG AGGAGTGCTG TGAGGATGAC AGTTCTCCCA CCTGGAGCAG GCAAAACTAC 
AGCTATTACA ACAGGTACCC AGGCAGCTCC ATGGACTTTG AGAGGCCCCG AGGCTACCAT 
CACCCCCAAG GTTTCTTAGA AGATGACGAC TCTCCCATTG GCTATGACTC ACGGAGATCT 
CCAAGAAGAC GCCTTCTACC TCCTACCCCA CCATCCCATC GGAGGTCCTC CTTCAACTTT 
GAGTGTCTGC GCAGGCAAAA CAGCCAAGAT GACGTCCTTC CGTCGCCTGC CCTGCCTCAC 
CGCGCTGCCC TGCCCCTGCA CCTTATGCAG CAGCAGATCA TGGCCGTTGC AGGCCTAGAT 
TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC CACTCTACCC GGTCATGGGC CACCCCTCCA 
GCAACCCCTC CGTACCGAGA CTGGACCCCA TGCTACACCC CTTTGATCCA GGTGGATCGC 
TCAGAGTCTA TGGACCAGGT CAATGGCAGC CTGCCGTCGC TACACCGCAG TTCCTGGTAT 
ACAGATGAAC CCGACATCTC CTATAGGACT TTCACACCGG CCAGCCTGAC CGTCCCCAGC 
AGCTTCCGGA ACAAAAACAG CGACAAGCAG AGGAGTGCAG ACAGCCTGGT GGAAGCAGTC 
CTGATATCCG AAGGCTTGGG ACGCTACGCA AGGGACCCAA AATTTGTCTC AGCAACAAAA 
CATGAGATCG CCGATGCCTG TGACCTAACC ATAGACGAGA TGGAGAGCGC GGCCAGCACC 
CTGCTCAATG GAAGCGTGTG TCCTCGAGCC AACGGGGACA TGGGCCCCAT CTCACACCGC 
CAGGACTATG AGCTCCAGGA CTTTGGTCCT GGCTACAGTG ACGAGGAACC AGACCCTGGG 
CGAGAGGAAG AGGACCTGGC AGATGAAATG ATTTGCATCA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455554.1
CDS	417..6827
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017600065.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600065.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGGTTGG TGAAGCTTCT CAGCAGAGGG GAAGGCATCC GGACTCTGCT ATGGACCTTC 
ATTAAGTCCT TCCAGGCACT CCCATATGTC GCCCTCCTCA TTGCCATGCT CTTCTTCATC 
TACGCCGTCA TTGGCATGCA GATGTTTGGG AAGGTTGCCA TGAGAGATAA CAACCAGATC 
AATAGGAACA ACAACTTCCA GACGTTTCCC CAGGCAGTGC TGCTGCTCTT CAGGTGTGCA 
ACAGGGGAGG CCTGGCAGGA GATCATGCTC GCCTGCCTCC CTGGGAAGCT GTGTGACCCG 
GACTCAGATT ACAACCCAGG AGAGGAATAT ACTTGTGGGA GCAACTTTGC CATTGTCTAC 
TTCATCAGCT TTTACATGCT CTGTGCGTTC CTGATCATCA ACCTCTTCGT GGCTGTCATC 
ATGGACAATT TTGACTATCT GACGAGGGAC TGGTCTATTC TGGGGCCTCA CCACTTGGAC 
GAATTCAAAA GAATATGGTC TGAATATGAC CCCGAGGCAA AGGGAAGGAT AAAACACCTT 
GATGTGGTCA CTTTGCTCCG ACGTATCCAG CCTCCCCTGG GATTTGGAAA GTTGTGTCCA 
CACCGAGTGG CGTGTAAGAG ATTGGTCGCC ATGAACATGC CTCTCAACAG TGATGGGACA 
GTCATGTTCA ATGCAACTCT GTTTGCTTTG GTACGGACGG CTCTCAAGAT CAAGACTGAA 
GGCAACCTGG AGCAAGCTAA TGAAGAGCTC CGCGCTGTGA TAAAGAAAAT CTGGAAGAAG 
ACAAGCATGA AGCTACTTGA CCAAGTTGTC CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAGCGGAAA 
GAGCAAGGCC TGGTGGGGAA ATACCCTGCG AAGAACACCA CGATCGCCCT ACAGGCGGGA 
TTAAGGACCC TGCATGACAT TGGGCCAGAA ATCCGACGGG CTATATCCTG TGACTTGCAA 
GATGACGAAC CAGAAGACTC CAAACCAGAA GAGGAAGATG TATTCAAAAG AAATGGTGCC 
CTGCTTGGAA ACTATGTCAA TCATGTTAAT AGTGATAGGA GAGAGTCCCT TCAGCAGACC 
AATACCACCC ACCGTCCCCT GCATGTCCAA AGGCCTTCAA TTCCACCTGC AAGTGATACT 
GAGAAACCGC TGTTTCCTCC AGCAGGAAAT TCGGTGTGTC ATAACCATCA TAACCATAAT 
TCCATAGGGA AGCAAGTTCC CACCTCCACA AATGCCAATC TCAATAACGC CAATATGTCC 
AAAGCTGCCC ACGGAAAGCG GCCCAGCATT GGGGACCTTG AGCATGTGTC TGAAAACGGC 
CATTACTCCT ACAAGCACGA CCGAGAGCTT CAAAGAAGGT CCAGTATTAA AAGAACCCGC 
TATTATGAAA CTTACATTAG GTCTGAGTCA GGAGACGAGC AGCTTCCAAC CATTTGCCGT 
GAAGACCCTG AGATACACGG CTACTTCCGA GACCCCCGCT GCTTCGGGGA GCAGGAATAT 
TTCAGTAGCG AGGAGTGCTG TGAGGATGAC AGTTCTCCCA CCTGGAGCAG GCAAAACTAC 
AGCTATTACA ACAGGTACCC AGGCAGCTCC ATGGACTTTG AGAGGCCCCG AGGCTACCAT 
CACCCCCAAG GTTTCTTAGA AGATGACGAC TCTCCCATTG GCTATGACTC ACGGAGATCT 
CCAAGAAGAC GCCTTCTACC TCCTACCCCA CCATCCCATC GGAGGTCCTC CTTCAACTTT 
GAGTGTCTGC GCAGGCAAAA CAGCCAAGAT GACGTCCTTC CGTCGCCTGC CCTGCCTCAC 
CGCGCTGCCC TGCCCCTGCA CCTTATGCAG CAGCAGATCA TGGCCGTTGC AGGCCTAGAT 
TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC CACTCTACCC GGTCATGGGC CACCCCTCCA 
GCAACCCCTC CGTACCGAGA CTGGACCCCA TGCTACACCC CTTTGATCCA GGTGGATCGC 
TCAGAGTCTA TGGACCAGGT CAATGGCAGC CTGCCGTCGC TACACCGCAG TTCCTGGTAT 
ACAGATGAAC CCGACATCTC CTATAGGACT TTCACACCGG CCAGCCTGAC CGTCCCCAGC 
AGCTTCCGGA ACAAAAACAG CGACAAGCAG AGGAGTGCAG ACAGCCTGGT GGAAGCAGTC 
CTGATATCCG AAGGCTTGGG ACGCTACGCA AGGGACCCAA AATTTGTCTC AGCAACAAAA 
CATGAGATCG CCGATGCCTG TGACCTAACC ATAGACGAGA TGGAGAGCGC GGCCAGCACC 
CTGCTCAATG GAAGCGTGTG TCCTCGAGCC AACGGGGACA TGGGCCCCAT CTCACACCGC 
CAGGACTATG AGCTCCAGGA CTTTGGTCCT GGCTACAGTG ACGAGGAACC AGACCCTGGG 
CGAGAGGAAG AGGACCTGGC AGATGAAATG ATTTGCATCA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600068.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455557.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X20, mRNA.

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CloneID	ORa58791
Clone ID Related Accession (Same CDS sequence)	XM_017600068.1
Accession Version	XM_017600068.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAGCTTCTCA GCAGAGGGGA AGGCATCCGG ACTCTGCTAT GGACCTTCAT TAAGTCCTTC 
CAGGCACTCC CATATGTCGC CCTCCTCATT GCCATGCTCT TCTTCATCTA CGCCGTCATT 
GGCATGCAGA TGTTTGGGAA GGTTGCCATG AGAGATAACA ACCAGATCAA TAGGAACAAC 
AACTTCCAGA CGTTTCCCCA GGCAGTGCTG CTGCTCTTCA GGTGTGCAAC AGGGGAGGCC 
TGGCAGGAGA TCATGCTCGC CTGCCTCCCT GGGAAGCTGT GTGACCCGGA CTCAGATTAC 
AACCCAGGAG AGGAATATAC TTGTGGGAGC AACTTTGCCA TTGTCTACTT CATCAGCTTT 
TACATGCTCT GTGCGTTCCT GATCATCAAC CTCTTCGTGG CTGTCATCAT GGACAATTTT 
GACTATCTGA CGAGGGACTG GTCTATTCTG GGGCCTCACC ACTTGGACGA ATTCAAAAGA 
ATATGGTCTG AATATGACCC CGAGGCAAAG GGAAGGATAA AACACCTTGA TGTGGTCACT 
TTGCTCCGAC GTATCCAGCC TCCCCTGGGA TTTGGAAAGT TGTGTCCACA CCGAGTGGCG 
TGTAAGAGAT TGGTCGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTCAAT 
GCAACTCTGT TTGCTTTGGT ACGGACGGCT CTCAAGATCA AGACTGAAGG CAACCTGGAG 
CAAGCTAATG AAGAGCTCCG CGCTGTGATA AAGAAAATCT GGAAGAAGAC AAGCATGAAG 
CTACTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AGCGGAAAGA GCAAGGCCTG 
GTGGGGAAAT ACCCTGCGAA GAACACCACG ATCGCCCTAC AGGCGGGATT AAGGACCCTG 
CATGACATTG GGCCAGAAAT CCGACGGGCT ATATCCTGTG ACTTGCAAGA TGACGAACCA 
GAAGACTCCA AACCAGAAGA GGAAGATGTA TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC 
TATGTCAATC ATGTTAATAG TGATAGGAGA GAGTCCCTTC AGCAGACCAA TACCACCCAC 
CGTCCCCTGC ATGTCCAAAG GCCTTCAATT CCACCTGCAA GTGATACTGA GAAACCGCTG 
TTTCCTCCAG CAGGAAATTC GGTGTGTCAT AACCATCATA ACCATAATTC CATAGGGAAG 
CAAGTTCCCA CCTCCACAAA TGCCAATCTC AATAACGCCA ATATGTCCAA AGCTGCCCAC 
GGAAAGCGGC CCAGCATTGG GGACCTTGAG CATGTGTCTG AAAACGGCCA TTACTCCTAC 
AAGCACGACC GAGAGCTTCA AAGAAGGTCC AGTATTAAAA GAACCCGCTA TTATGAAACT 
TACATTAGGT CTGAGTCAGG AGACGAGCAG CTTCCAACCA TTTGCCGTGA AGACCCTGAG 
ATACACGGCT ACTTCCGAGA CCCCCGCTGC TTCGGGGAGC AGGAATATTT CAGTAGCGAG 
GAGTGCTGTG AGGATGACAG TTCTCCCACC TGGAGCAGGC AAAACTACAG CTATTACAAC 
AGGTACCCAG GCAGCTCCAT GGACTTTGAG AGGCCCCGAG GCTACCATCA CCCCCAAGGT 
TTCTTAGAAG ATGACGACTC TCCCATTGGC TATGACTCAC GGAGATCTCC AAGAAGACGC 
CTTCTACCTC CTACCCCACC ATCCCATCGG AGGTCCTCCT TCAACTTTGA GTGTCTGCGC 
AGGCAAAACA GCCAAGATGA CGTCCTTCCG TCGCCTGCCC TGCCTCACCG CGCTGCCCTG 
CCCCTGCACC TTATGCAGCA GCAGATCATG GCCGTTGCAG GCCTAGATTC CAGTAAAGCC 
CAGAAGTACT CGCCGAGCCA CTCTACCCGG TCATGGGCCA CCCCTCCAGC AACCCCTCCG 
TACCGAGACT GGACCCCATG CTACACCCCT TTGATCCAGG TGGATCGCTC AGAGTCTATG 
GACCAGGTCA ATGGCAGCCT GCCGTCGCTA CACCGCAGTT CCTGGTATAC AGATGAACCC 
GACATCTCCT ATAGGACTTT CACACCGGCC AGCCTGACCG TCCCCAGCAG CTTCCGGAAC 
AAAAACAGCG ACAAGCAGAG GAGTGCAGAC AGCCTGGTGG AAGCAGTCCT GATATCCGAA 
GGCTTGGGAC GCTACGCAAG GGACCCAAAA TTTGTCTCAG CAACAAAACA TGAGATCGCC 
GATGCCTGTG ACCTAACCAT AGACGAGATG GAGAGCGCGG CCAGCACCCT GCTCAATGGA 
AGCGTGTGTC CTCGAGCCAA CGGGGACATG GGCCCCATCT CACACCGCCA GGACTATGAG 
CTCCAGGACT TTGGTCCTGG CTACAGTGAC GAGGAACCAG ACCCTGGGCG AGAGGAAGAG 
GACCTGGCAG ATGAAATGAT TTGCATCACC ACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455557.1
CDS	417..6935
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017600068.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600068.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CACTATTTCA CTGATGCATG GAATACTTTT 
GATGCCTTAA TTGTTGTCGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAGCTTCTCA GCAGAGGGGA AGGCATCCGG ACTCTGCTAT GGACCTTCAT TAAGTCCTTC 
CAGGCACTCC CATATGTCGC CCTCCTCATT GCCATGCTCT TCTTCATCTA CGCCGTCATT 
GGCATGCAGA TGTTTGGGAA GGTTGCCATG AGAGATAACA ACCAGATCAA TAGGAACAAC 
AACTTCCAGA CGTTTCCCCA GGCAGTGCTG CTGCTCTTCA GGTGTGCAAC AGGGGAGGCC 
TGGCAGGAGA TCATGCTCGC CTGCCTCCCT GGGAAGCTGT GTGACCCGGA CTCAGATTAC 
AACCCAGGAG AGGAATATAC TTGTGGGAGC AACTTTGCCA TTGTCTACTT CATCAGCTTT 
TACATGCTCT GTGCGTTCCT GATCATCAAC CTCTTCGTGG CTGTCATCAT GGACAATTTT 
GACTATCTGA CGAGGGACTG GTCTATTCTG GGGCCTCACC ACTTGGACGA ATTCAAAAGA 
ATATGGTCTG AATATGACCC CGAGGCAAAG GGAAGGATAA AACACCTTGA TGTGGTCACT 
TTGCTCCGAC GTATCCAGCC TCCCCTGGGA TTTGGAAAGT TGTGTCCACA CCGAGTGGCG 
TGTAAGAGAT TGGTCGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTCAAT 
GCAACTCTGT TTGCTTTGGT ACGGACGGCT CTCAAGATCA AGACTGAAGG CAACCTGGAG 
CAAGCTAATG AAGAGCTCCG CGCTGTGATA AAGAAAATCT GGAAGAAGAC AAGCATGAAG 
CTACTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AGCGGAAAGA GCAAGGCCTG 
GTGGGGAAAT ACCCTGCGAA GAACACCACG ATCGCCCTAC AGGCGGGATT AAGGACCCTG 
CATGACATTG GGCCAGAAAT CCGACGGGCT ATATCCTGTG ACTTGCAAGA TGACGAACCA 
GAAGACTCCA AACCAGAAGA GGAAGATGTA TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC 
TATGTCAATC ATGTTAATAG TGATAGGAGA GAGTCCCTTC AGCAGACCAA TACCACCCAC 
CGTCCCCTGC ATGTCCAAAG GCCTTCAATT CCACCTGCAA GTGATACTGA GAAACCGCTG 
TTTCCTCCAG CAGGAAATTC GGTGTGTCAT AACCATCATA ACCATAATTC CATAGGGAAG 
CAAGTTCCCA CCTCCACAAA TGCCAATCTC AATAACGCCA ATATGTCCAA AGCTGCCCAC 
GGAAAGCGGC CCAGCATTGG GGACCTTGAG CATGTGTCTG AAAACGGCCA TTACTCCTAC 
AAGCACGACC GAGAGCTTCA AAGAAGGTCC AGTATTAAAA GAACCCGCTA TTATGAAACT 
TACATTAGGT CTGAGTCAGG AGACGAGCAG CTTCCAACCA TTTGCCGTGA AGACCCTGAG 
ATACACGGCT ACTTCCGAGA CCCCCGCTGC TTCGGGGAGC AGGAATATTT CAGTAGCGAG 
GAGTGCTGTG AGGATGACAG TTCTCCCACC TGGAGCAGGC AAAACTACAG CTATTACAAC 
AGGTACCCAG GCAGCTCCAT GGACTTTGAG AGGCCCCGAG GCTACCATCA CCCCCAAGGT 
TTCTTAGAAG ATGACGACTC TCCCATTGGC TATGACTCAC GGAGATCTCC AAGAAGACGC 
CTTCTACCTC CTACCCCACC ATCCCATCGG AGGTCCTCCT TCAACTTTGA GTGTCTGCGC 
AGGCAAAACA GCCAAGATGA CGTCCTTCCG TCGCCTGCCC TGCCTCACCG CGCTGCCCTG 
CCCCTGCACC TTATGCAGCA GCAGATCATG GCCGTTGCAG GCCTAGATTC CAGTAAAGCC 
CAGAAGTACT CGCCGAGCCA CTCTACCCGG TCATGGGCCA CCCCTCCAGC AACCCCTCCG 
TACCGAGACT GGACCCCATG CTACACCCCT TTGATCCAGG TGGATCGCTC AGAGTCTATG 
GACCAGGTCA ATGGCAGCCT GCCGTCGCTA CACCGCAGTT CCTGGTATAC AGATGAACCC 
GACATCTCCT ATAGGACTTT CACACCGGCC AGCCTGACCG TCCCCAGCAG CTTCCGGAAC 
AAAAACAGCG ACAAGCAGAG GAGTGCAGAC AGCCTGGTGG AAGCAGTCCT GATATCCGAA 
GGCTTGGGAC GCTACGCAAG GGACCCAAAA TTTGTCTCAG CAACAAAACA TGAGATCGCC 
GATGCCTGTG ACCTAACCAT AGACGAGATG GAGAGCGCGG CCAGCACCCT GCTCAATGGA 
AGCGTGTGTC CTCGAGCCAA CGGGGACATG GGCCCCATCT CACACCGCCA GGACTATGAG 
CTCCAGGACT TTGGTCCTGG CTACAGTGAC GAGGAACCAG ACCCTGGGCG AGAGGAAGAG 
GACCTGGCAG ATGAAATGAT TTGCATCACC ACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600069.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455558.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X21, mRNA.

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CloneID	ORa58792
Clone ID Related Accession (Same CDS sequence)	XM_017600069.1
Accession Version	XM_017600069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAT ACTTTTGATG CCTTAATTGT TGTCGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAGC TTCTCAGCAG AGGGGAAGGC 
ATCCGGACTC TGCTATGGAC CTTCATTAAG TCCTTCCAGG CACTCCCATA TGTCGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCC GTCATTGGCA TGCAGATGTT TGGGAAGGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACGTT TCCCCAGGCA 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGG GAGGCCTGGC AGGAGATCAT GCTCGCCTGC 
CTCCCTGGGA AGCTGTGTGA CCCGGACTCA GATTACAACC CAGGAGAGGA ATATACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC GTTCCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACGAG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCTGAATA TGACCCCGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTTTGC TCCGACGTAT CCAGCCTCCC 
CTGGGATTTG GAAAGTTGTG TCCACACCGA GTGGCGTGTA AGAGATTGGT CGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CTCTGTTTGC TTTGGTACGG 
ACGGCTCTCA AGATCAAGAC TGAAGGCAAC CTGGAGCAAG CTAATGAAGA GCTCCGCGCT 
GTGATAAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAGCG GAAAGAGCAA GGCCTGGTGG GGAAATACCC TGCGAAGAAC 
ACCACGATCG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAACCAGAAG ACTCCAAACC AGAAGAGGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACTATG TCAATCATGT TAATAGTGAT 
AGGAGAGAGT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATTCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGTG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG TTCCCACCTC CACAAATGCC 
AATCTCAATA ACGCCAATAT GTCCAAAGCT GCCCACGGAA AGCGGCCCAG CATTGGGGAC 
CTTGAGCATG TGTCTGAAAA CGGCCATTAC TCCTACAAGC ACGACCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAT GAAACTTACA TTAGGTCTGA GTCAGGAGAC 
GAGCAGCTTC CAACCATTTG CCGTGAAGAC CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTCG GGGAGCAGGA ATATTTCAGT AGCGAGGAGT GCTGTGAGGA TGACAGTTCT 
CCCACCTGGA GCAGGCAAAA CTACAGCTAT TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCATCACCCC CAAGGTTTCT TAGAAGATGA CGACTCTCCC 
ATTGGCTATG ACTCACGGAG ATCTCCAAGA AGACGCCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAACAGCCA AGATGACGTC 
CTTCCGTCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTTAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCGCC GAGCCACTCT 
ACCCGGTCAT GGGCCACCCC TCCAGCAACC CCTCCGTACC GAGACTGGAC CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA TCGCTCAGAG TCTATGGACC AGGTCAATGG CAGCCTGCCG 
TCGCTACACC GCAGTTCCTG GTATACAGAT GAACCCGACA TCTCCTATAG GACTTTCACA 
CCGGCCAGCC TGACCGTCCC CAGCAGCTTC CGGAACAAAA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAAGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCAAGGGAC 
CCAAAATTTG TCTCAGCAAC AAAACATGAG ATCGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAGA GCGCGGCCAG CACCCTGCTC AATGGAAGCG TGTGTCCTCG AGCCAACGGG 
GACATGGGCC CCATCTCACA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AACCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA AATGATTTGC 
ATCACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455558.1
CDS	417..6851
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017600069.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600069.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAT ACTTTTGATG CCTTAATTGT TGTCGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAGC TTCTCAGCAG AGGGGAAGGC 
ATCCGGACTC TGCTATGGAC CTTCATTAAG TCCTTCCAGG CACTCCCATA TGTCGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCC GTCATTGGCA TGCAGATGTT TGGGAAGGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACGTT TCCCCAGGCA 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGG GAGGCCTGGC AGGAGATCAT GCTCGCCTGC 
CTCCCTGGGA AGCTGTGTGA CCCGGACTCA GATTACAACC CAGGAGAGGA ATATACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC GTTCCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACGAG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCTGAATA TGACCCCGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTTTGC TCCGACGTAT CCAGCCTCCC 
CTGGGATTTG GAAAGTTGTG TCCACACCGA GTGGCGTGTA AGAGATTGGT CGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CTCTGTTTGC TTTGGTACGG 
ACGGCTCTCA AGATCAAGAC TGAAGGCAAC CTGGAGCAAG CTAATGAAGA GCTCCGCGCT 
GTGATAAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAGCG GAAAGAGCAA GGCCTGGTGG GGAAATACCC TGCGAAGAAC 
ACCACGATCG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAACCAGAAG ACTCCAAACC AGAAGAGGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACTATG TCAATCATGT TAATAGTGAT 
AGGAGAGAGT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATTCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGTG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG TTCCCACCTC CACAAATGCC 
AATCTCAATA ACGCCAATAT GTCCAAAGCT GCCCACGGAA AGCGGCCCAG CATTGGGGAC 
CTTGAGCATG TGTCTGAAAA CGGCCATTAC TCCTACAAGC ACGACCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAT GAAACTTACA TTAGGTCTGA GTCAGGAGAC 
GAGCAGCTTC CAACCATTTG CCGTGAAGAC CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTCG GGGAGCAGGA ATATTTCAGT AGCGAGGAGT GCTGTGAGGA TGACAGTTCT 
CCCACCTGGA GCAGGCAAAA CTACAGCTAT TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCATCACCCC CAAGGTTTCT TAGAAGATGA CGACTCTCCC 
ATTGGCTATG ACTCACGGAG ATCTCCAAGA AGACGCCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAACAGCCA AGATGACGTC 
CTTCCGTCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTTAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCGCC GAGCCACTCT 
ACCCGGTCAT GGGCCACCCC TCCAGCAACC CCTCCGTACC GAGACTGGAC CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA TCGCTCAGAG TCTATGGACC AGGTCAATGG CAGCCTGCCG 
TCGCTACACC GCAGTTCCTG GTATACAGAT GAACCCGACA TCTCCTATAG GACTTTCACA 
CCGGCCAGCC TGACCGTCCC CAGCAGCTTC CGGAACAAAA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAAGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCAAGGGAC 
CCAAAATTTG TCTCAGCAAC AAAACATGAG ATCGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAGA GCGCGGCCAG CACCCTGCTC AATGGAAGCG TGTGTCCTCG AGCCAACGGG 
GACATGGGCC CCATCTCACA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AACCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA AATGATTTGC 
ATCACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017600070.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455559.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X22, mRNA.

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CloneID	ORa58793
Clone ID Related Accession (Same CDS sequence)	XM_017600070.1
Accession Version	XM_017600070.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTGCTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAT ACTTTTGATG CCTTAATTGT TGTCGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAGC TTCTCAGCAG AGGGGAAGGC 
ATCCGGACTC TGCTATGGAC CTTCATTAAG TCCTTCCAGG CACTCCCATA TGTCGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCC GTCATTGGCA TGCAGATGTT TGGGAAGGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACGTT TCCCCAGGCA 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGG GAGGCCTGGC AGGAGATCAT GCTCGCCTGC 
CTCCCTGGGA AGCTGTGTGA CCCGGACTCA GATTACAACC CAGGAGAGGA ATATACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC GTTCCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACGAG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCTGAATA TGACCCCGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTTTGC TCCGACGTAT CCAGCCTCCC 
CTGGGATTTG GAAAGTTGTG TCCACACCGA GTGGCGTGTA AGAGATTGGT CGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CTCTGTTTGC TTTGGTACGG 
ACGGCTCTCA AGATCAAGAC TGAAGGCAAC CTGGAGCAAG CTAATGAAGA GCTCCGCGCT 
GTGATAAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAGCG GAAAGAGCAA GGCCTGGTGG GGAAATACCC TGCGAAGAAC 
ACCACGATCG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAACCAGAAG ACTCCAAACC AGAAGAGGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACTATG TCAATCATGT TAATAGTGAT 
AGGAGAGAGT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATTCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGTG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG TTCCCACCTC CACAAATGCC 
AATCTCAATA ACGCCAATAT GTCCAAAGCT GCCCACGGAA AGCGGCCCAG CATTGGGGAC 
CTTGAGCATG TGTCTGAAAA CGGCCATTAC TCCTACAAGC ACGACCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAT GAAACTTACA TTAGGTCTGA GTCAGGAGAC 
GAGCAGCTTC CAACCATTTG CCGTGAAGAC CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTCG GGGAGCAGGA ATATTTCAGT AGCGAGGAGT GCTGTGAGGA TGACAGTTCT 
CCCACCTGGA GCAGGCAAAA CTACAGCTAT TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCATCACCCC CAAGGTTTCT TAGAAGATGA CGACTCTCCC 
ATTGGCTATG ACTCACGGAG ATCTCCAAGA AGACGCCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAACAGCCA AGATGACGTC 
CTTCCGTCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTTAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCGCC GAGCCACTCT 
ACCCGGTCAT GGGCCACCCC TCCAGCAACC CCTCCGTACC GAGACTGGAC CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA TCGCTCAGAG TCTATGGACC AGGTCAATGG CAGCCTGCCG 
TCGCTACACC GCAGTTCCTG GTATACAGAT GAACCCGACA TCTCCTATAG GACTTTCACA 
CCGGCCAGCC TGACCGTCCC CAGCAGCTTC CGGAACAAAA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAAGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCAAGGGAC 
CCAAAATTTG TCTCAGCAAC AAAACATGAG ATCGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAGA GCGCGGCCAG CACCCTGCTC AATGGAAGCG TGTGTCCTCG AGCCAACGGG 
GACATGGGCC CCATCTCACA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AACCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA AATGATTTGC 
ATCACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455559.1
CDS	417..6851
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017600070.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600070.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTGCTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAT ACTTTTGATG CCTTAATTGT TGTCGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAGC TTCTCAGCAG AGGGGAAGGC 
ATCCGGACTC TGCTATGGAC CTTCATTAAG TCCTTCCAGG CACTCCCATA TGTCGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCC GTCATTGGCA TGCAGATGTT TGGGAAGGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACGTT TCCCCAGGCA 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGG GAGGCCTGGC AGGAGATCAT GCTCGCCTGC 
CTCCCTGGGA AGCTGTGTGA CCCGGACTCA GATTACAACC CAGGAGAGGA ATATACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC GTTCCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACGAG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCTGAATA TGACCCCGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTTTGC TCCGACGTAT CCAGCCTCCC 
CTGGGATTTG GAAAGTTGTG TCCACACCGA GTGGCGTGTA AGAGATTGGT CGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CTCTGTTTGC TTTGGTACGG 
ACGGCTCTCA AGATCAAGAC TGAAGGCAAC CTGGAGCAAG CTAATGAAGA GCTCCGCGCT 
GTGATAAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAGCG GAAAGAGCAA GGCCTGGTGG GGAAATACCC TGCGAAGAAC 
ACCACGATCG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAACCAGAAG ACTCCAAACC AGAAGAGGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACTATG TCAATCATGT TAATAGTGAT 
AGGAGAGAGT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATTCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGTG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG TTCCCACCTC CACAAATGCC 
AATCTCAATA ACGCCAATAT GTCCAAAGCT GCCCACGGAA AGCGGCCCAG CATTGGGGAC 
CTTGAGCATG TGTCTGAAAA CGGCCATTAC TCCTACAAGC ACGACCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAT GAAACTTACA TTAGGTCTGA GTCAGGAGAC 
GAGCAGCTTC CAACCATTTG CCGTGAAGAC CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTCG GGGAGCAGGA ATATTTCAGT AGCGAGGAGT GCTGTGAGGA TGACAGTTCT 
CCCACCTGGA GCAGGCAAAA CTACAGCTAT TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCATCACCCC CAAGGTTTCT TAGAAGATGA CGACTCTCCC 
ATTGGCTATG ACTCACGGAG ATCTCCAAGA AGACGCCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAACAGCCA AGATGACGTC 
CTTCCGTCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTTAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCGCC GAGCCACTCT 
ACCCGGTCAT GGGCCACCCC TCCAGCAACC CCTCCGTACC GAGACTGGAC CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA TCGCTCAGAG TCTATGGACC AGGTCAATGG CAGCCTGCCG 
TCGCTACACC GCAGTTCCTG GTATACAGAT GAACCCGACA TCTCCTATAG GACTTTCACA 
CCGGCCAGCC TGACCGTCCC CAGCAGCTTC CGGAACAAAA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAAGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCAAGGGAC 
CCAAAATTTG TCTCAGCAAC AAAACATGAG ATCGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAGA GCGCGGCCAG CACCCTGCTC AATGGAAGCG TGTGTCCTCG AGCCAACGGG 
GACATGGGCC CCATCTCACA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AACCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA AATGATTTGC 
ATCACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( NM_017298.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058994.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), mRNA.

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CloneID	ORa12532
Clone ID Related Accession (Same CDS sequence)	NM_017298.1
Accession Version	NM_017298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D38101.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D38101.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTCCG GACTGCTGCT GCATCCTAAT GCTTCCGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC GAAATCCAAG 
CTCAGATCGC ACGGGGCCCG CGAGGCTTTG TGTGTGTGCC GGTGCAGCCT TGAATCTCTC 
GTGAAACTAT GGACGTCTAG GTTCTCAGCC CACTTGCAGG CTGCATATGT CAGACCCTAC 
AGCCGTCGGT GGCGTCGCTG GAACCGGTTC AATCGCAGGA GATGCAGGGC AGCTGTGAAG 
TCTGTCACGT TTTACTGGCT GGTGATCGTT CTGGTGTTTC TCAACACTCT GACGATTTCC 
TCTGAGCACT ACAATCAGCC AGACTGGCTG ACGCAGATTC AAGATATAGC GAACAAAGTG 
CTCTTGGCTT TGTTCACCTG TGAGATGCTG GTGAAGATGT ACAGCCTGGG CCTCCAGGCC 
TACTTCGTCT CTCTCTTCAA CCGCTTCGAT TGCTTTGTGG TGTGTGGGGG AATCACCGAA 
ACCATCCTGG TGGAACTGGA GCTCATGTCT CCCCTGGGCG TCTCTGTGTT CCGGTGTGTA 
CGTCTCCTGA GGATCTTCAA AGTGACCAGG CACTGGACTT CCCTGAGCAA CTTGGTGGCG 
TCCCTGTTAA ACTCCATGAA GTCCATCGCC TCTCTGCTGT TGCTGCTCTT CCTCTTCATC 
ATCATCTTCT CCCTGCTGGG GATGCAGCTG TTCGGAGGGA AGTTCAATTT CGATGAGACA 
CAGACCAAGC GAAGCACCTT CGACAACTTC CCACAGGCGC TCCTGACTGT GTTCCAGATC 
CTGACGGGCG AAGACTGGAA TGCGGTGATG TATGACGGCA TCATGGCCTA CGGAGGCCCG 
TCCTCCTCTG GAATGATTGT CTGTATTTAC TTCATCATCC TTTTCATCTG TGGTAACTAT 
ATCCTGCTGA AGCTCTTCTT GGCCATTGCT GTAGACAATT TGGCTGATGC TGAAAGTCTG 
AACACTGCTC AGAAAGAGGA AGCCGAAGAA AAAGAAAGGA AAAAAATTGC CAGAAAAGAA 
AGCCTAGAAA ACAAAAAAAA CAACAAACCA GAGGTCAACC AGATAGCCAA CAGTGACAAC 
AAGGTTACTA TTGATGACTA TCAAGAGGAG GCTGAAGATA AGGACCCTTA TCCACCCTGC 
GATGTGCCAG TAGGTGAAGA GGAAGAGGAG GAGGAAGAGG ACGAGCCTGA GGTTCCTGCT 
GGTCCCCGTC CTCGAAGAAT CTCAGAGTTG AACATGAAGG AGAAAATTGC GCCCATCCCC 
GAAGGAAGCG CATTCTTCAT TCTTAGCAAG ACCAACCCGA TCCGTGTGGG TTGCCACAAA 
CTCATCAACC ACCACATCTT CACCAACCTC ATCCTGGTCT TCATCATGCT GAGCAGCGCT 
GCCCTGGCCG CAGAGGATCC CATCCGCAGC CACTCCTTCC GCAACACTAT ACTGGGCTAC 
TTTGACTATG CTTTCACAGC CATCTTTACG GTTGAAATCC TGTTAAAGAT GACAACTTTT 
GGAGCGTTCT TGCACAAAGG GGCTTTCTGT AGGAACTACT TCAATTTGCT GGACATGCTG 
GTCGTTGGGG TGTCTCTGGT GTCATTTGGG ATTCAATCCA GTGCCATCTC GGTTGTGAAG 
ATTCTGAGGG TCTTAAGGGT CTTGAGGCCT CTCAGAGCAA TCAACAGAGC AAAAGGACTT 
AAGCACGTGG TCCAGTGTGT CTTTGTGGCC ATCCGAACCA TCGGCAACAT CATGATCGTC 
ACGACCCTGC TCCAGTTCAT GTTTGCTTGC ATTGGGGTCC AGCTGTTCAA GGGGAAGTTC 
TACCGTTGCA CAGATGAGGC CAAAAGTAAC CCCGAGGAGT GCAGGGGGCT TTTCATCCTT 
TATAAGGACG GCGATGTCGA CAGTCCCGTG GTCCGTGAGA GGATCTGGCA AAACAGTGAT 
TTCAATTTCG ACAATGTCCT TTCGGCTATG ATGGCGCTCT TCACGGTCTC GACTTTTGAG 
GGCTGGCCCG CGTTGCTGTA CAAAGCTATC GACTCAAACG GAGAGAACGT TGGTCCTGTC 
TACAACTACC GTGTGGAGAT CTCCATCTTC TTCATTATCT ACATCATCAT CGTGGCCTTC 
TTCATGATGA ATATCTTCGT GGGCTTCGTC ATCGTCACCT TCCAGGAGCA GGGAGAAAAG 
GAGTATAAGA ACTGTGAGCT GGACAAAAAT CAGCGTCAGT GTGTGGAATA TGCCTTGAAG 
GCCCGCCCCT TAAGGAGATA CATCCCCAAA AACCCATACC AGTACAAGTT CTGGTACGTG 
GTGAACTCCT CGCCTTTCGA ATATATGATG TTTGTCCTCA TCATGCTCAA CACGCTCTGC 
CTGGCCATGC AGCACTATGA GCAATCCAAG ATGTTCAATG ACGCCATGGA CATTCTGAAC 
ATGGTCTTCA CGGGGGTCTT CACCGTTGAG ATGGTTTTGA AAGTCATCGC ATTTAAGCCC 
AAGGGGTATT TTAGTGACGC CTGGAACACG TTTGACTCCC TCATCGTAAT CGGCAGCATT 
ATAGACGTGG CACTCAGCGA AGCTGACAAC TCTGAAGAGA GCAATAGAAT CTCCATCACC 
TTTTTCCGTC TTTTCCGAGT GATGCGGTTG GTGAAGCTTC TCAGCAGAGG GGAAGGCATC 
CGGACTCTGC TATGGACCTT CATTAAGTCC TTCCAGGCAC TCCCATATGT CGCCCTCCTC 
ATTGCCATGC TCTTCTTCAT CTACGCCGTC ATTGGCATGC AGATGTTTGG GAAGGTTGCC 
ATGAGAGATA ACAACCAGAT CAATAGGAAC AACAACTTCC AGACGTTTCC CCAGGCAGTG 
CTGCTGCTCT TCAGGTGTGC AACAGGGGAG GCCTGGCAGG AGATCATGCT CGCCTGCCTC 
CCTGGGAAGC TGTGTGACCC GGACTCAGAT TACAACCCAG GAGAGGAATA TACTTGTGGG 
AGCAACTTTG CCATTGTCTA CTTCATCAGC TTTTACATGC TCTGTGCGTT CCTGATCATC 
AACCTCTTCG TGGCTGTCAT CATGGACAAT TTTGACTATC TGACGAGGGA CTGGTCTATT 
CTGGGGCCTC ACCACTTGGA CGAATTCAAA AGAATATGGT CTGAATATGA CCCCGAGGCA 
AAGGGAAGGA TAAAACACCT TGATGTGGTC ACTTTGCTCC GACGTATCCA GCCTCCCCTG 
GGATTTGGAA AGTTGTGTCC ACACCGAGTG GCGTGTAAGA GATTGGTCGC CATGAACATG 
CCTCTCAACA GTGATGGGAC AGTCATGTTC AATGCAACTC TGTTTGCTTT GGTACGGACG 
GCTCTCAAGA TCAAGACTGA AGGCAACCTG GAGCAAGCTA ATGAAGAGCT CCGCGCTGTG 
ATAAAGAAAA TCTGGAAGAA GACAAGCATG AAGCTACTTG ACCAAGTTGT CCCTCCAGCT 
GGTGATGATG AGGTAACCGT GGGGAAGTTC TATGCCACTT TCCTGATACA GGACTACTTT 
AGGAAATTCA AGAAGCGGAA AGAGCAAGGC CTGGTGGGGA AATACCCTGC GAAGAACACC 
ACGATCGCCC TACAGGCGGG ATTAAGGACC CTGCATGACA TTGGGCCAGA AATCCGACGG 
GCTATATCCT GTGACTTGCA AGATGACGAA CCAGAAGACT CCAAACCAGA AGAGGAAGAT 
GTATTCAAAA GAAATGGTGC CCTGCTTGGA AACTATGTCA ATCATGTTAA TAGTGATAGG 
AGAGAGTCCC TTCAGCAGAC CAATACCACC CACCGTCCCC TGCATGTCCA AAGGCCTTCA 
ATTCCACCTG CAAGTGATAC TGAGAAACCG CTGTTTCCTC CAGCAGGAAA TTCGGTGTGT 
CATAACCATC ATAACCATAA TTCCATAGGG AAGCAAGTTC CCACCTCCAC AAATGCCAAT 
CTCAATAACG CCAATATGTC CAAAGCTGCC CACGGAAAGC GGCCCAGCAT TGGGGACCTT 
GAGCATGTGT CTGAAAACGG CCATTACTCC TACAAGCACG ACCGAGAGCT TCAAAGAAGG 
TCCAGTATTA AAAGAACCCG CTATTATGAA ACTTACATTA GGTCTGAGTC AGGAGACGAG 
CAGCTTCCAA CCATTTTCCG TGAAGACCCT GAGATACACG GCTACTTCCG AGACCCCCGC 
TGCTTCGGGG AGCAGGAATA TTTCAGTAGC GAGGAGTGCT GTGAGGATGA CAGTTCTCCC 
ACCTGGAGCA GGCAAAACTA CAGCTATTAC AACAGGTACC CAGGCAGCTC CATGGACTTT 
GAGAGGCCCC GAGGCTACCA TCACCCCCAA GGTTTCTTAG AAGATGACGA CTCTCCCATT 
GGCTATGACT CACGGAGATC TCCAAGAAGA CGCCTTCTAC CTCCTACCCC ACCATCCCAT 
CGGAGGTCCT CCTTCAACTT TGAGTGTCTG CGCAGGCAAA ACAGCCAAGA TGACGTCCTT 
CCGTCGCCTG CCCTGCCTCA CCGCGCTGCC CTGCCCCTGC ACCTTATGCA GCAGCAGATC 
ATGGCCGTTG CAGGCCTAGA TTCCAGTAAA GCCCAGAAGT ACTCGCCGAG CCACTCTACC 
CGGTCATGGG CCACCCCTCC AGCAACCCCT CCGTACCGAG ACTGGACCCC ATGCTACACC 
CCTTTGATCC AGGTGGATCG CTCAGAGTCT ATGGACCAGG TCAATGGCAG CCTGCCGTCG 
CTACACCGCA GTTCCTGGTA TACAGATGAA CCCGACATCT CCTATAGGAC TTTCACACCG 
GCCAGCCTGA CCGTCCCCAG CAGCTTCCGG AACAAAAACA GCGACAAGCA GAGGAGTGCA 
GACAGCCTGG TGGAAGCAGT CCTGATATCC GAAGGCTTGG GACGCTACGC AAGGGACCCA 
AAATTTGTCT CAGCAACAAA ACATGAGATC GCCGATGCCT GTGACCTAAC CATAGACGAG 
ATGGAGAGCG CGGCCAGCAC CCTGCTCAAT GGAAGCGTGT GTCCTCGAGC CAACGGGGAC 
ATGGGCCCCA TCTCACACCG CCAGGACTAT GAGCTCCAGG ACTTTGGTCC TGGCTACAGT 
GACGAGGAAC CAGACCCTGG GCGAGAGGAA GAGGACCTGG CAGATGAAAT GATTTGCATC 
ACCACCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058994.1
CDS	355..6966
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIASGLLLHPNASVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLWCWWKRRGAAKTGPSGCRRWGQAISKSKLRSHGAREALCVCRCSLESLVKLWTSRFSAHLQAAYVRPYSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLKLFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTIFREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	NM_017298.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017298.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTCCG GACTGCTGCT GCATCCTAAT GCTTCCGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC GAAATCCAAG 
CTCAGATCGC ACGGGGCCCG CGAGGCTTTG TGTGTGTGCC GGTGCAGCCT TGAATCTCTC 
GTGAAACTAT GGACGTCTAG GTTCTCAGCC CACTTGCAGG CTGCATATGT CAGACCCTAC 
AGCCGTCGGT GGCGTCGCTG GAACCGGTTC AATCGCAGGA GATGCAGGGC AGCTGTGAAG 
TCTGTCACGT TTTACTGGCT GGTGATCGTT CTGGTGTTTC TCAACACTCT GACGATTTCC 
TCTGAGCACT ACAATCAGCC AGACTGGCTG ACGCAGATTC AAGATATAGC GAACAAAGTG 
CTCTTGGCTT TGTTCACCTG TGAGATGCTG GTGAAGATGT ACAGCCTGGG CCTCCAGGCC 
TACTTCGTCT CTCTCTTCAA CCGCTTCGAT TGCTTTGTGG TGTGTGGGGG AATCACCGAA 
ACCATCCTGG TGGAACTGGA GCTCATGTCT CCCCTGGGCG TCTCTGTGTT CCGGTGTGTA 
CGTCTCCTGA GGATCTTCAA AGTGACCAGG CACTGGACTT CCCTGAGCAA CTTGGTGGCG 
TCCCTGTTAA ACTCCATGAA GTCCATCGCC TCTCTGCTGT TGCTGCTCTT CCTCTTCATC 
ATCATCTTCT CCCTGCTGGG GATGCAGCTG TTCGGAGGGA AGTTCAATTT CGATGAGACA 
CAGACCAAGC GAAGCACCTT CGACAACTTC CCACAGGCGC TCCTGACTGT GTTCCAGATC 
CTGACGGGCG AAGACTGGAA TGCGGTGATG TATGACGGCA TCATGGCCTA CGGAGGCCCG 
TCCTCCTCTG GAATGATTGT CTGTATTTAC TTCATCATCC TTTTCATCTG TGGTAACTAT 
ATCCTGCTGA AGCTCTTCTT GGCCATTGCT GTAGACAATT TGGCTGATGC TGAAAGTCTG 
AACACTGCTC AGAAAGAGGA AGCCGAAGAA AAAGAAAGGA AAAAAATTGC CAGAAAAGAA 
AGCCTAGAAA ACAAAAAAAA CAACAAACCA GAGGTCAACC AGATAGCCAA CAGTGACAAC 
AAGGTTACTA TTGATGACTA TCAAGAGGAG GCTGAAGATA AGGACCCTTA TCCACCCTGC 
GATGTGCCAG TAGGTGAAGA GGAAGAGGAG GAGGAAGAGG ACGAGCCTGA GGTTCCTGCT 
GGTCCCCGTC CTCGAAGAAT CTCAGAGTTG AACATGAAGG AGAAAATTGC GCCCATCCCC 
GAAGGAAGCG CATTCTTCAT TCTTAGCAAG ACCAACCCGA TCCGTGTGGG TTGCCACAAA 
CTCATCAACC ACCACATCTT CACCAACCTC ATCCTGGTCT TCATCATGCT GAGCAGCGCT 
GCCCTGGCCG CAGAGGATCC CATCCGCAGC CACTCCTTCC GCAACACTAT ACTGGGCTAC 
TTTGACTATG CTTTCACAGC CATCTTTACG GTTGAAATCC TGTTAAAGAT GACAACTTTT 
GGAGCGTTCT TGCACAAAGG GGCTTTCTGT AGGAACTACT TCAATTTGCT GGACATGCTG 
GTCGTTGGGG TGTCTCTGGT GTCATTTGGG ATTCAATCCA GTGCCATCTC GGTTGTGAAG 
ATTCTGAGGG TCTTAAGGGT CTTGAGGCCT CTCAGAGCAA TCAACAGAGC AAAAGGACTT 
AAGCACGTGG TCCAGTGTGT CTTTGTGGCC ATCCGAACCA TCGGCAACAT CATGATCGTC 
ACGACCCTGC TCCAGTTCAT GTTTGCTTGC ATTGGGGTCC AGCTGTTCAA GGGGAAGTTC 
TACCGTTGCA CAGATGAGGC CAAAAGTAAC CCCGAGGAGT GCAGGGGGCT TTTCATCCTT 
TATAAGGACG GCGATGTCGA CAGTCCCGTG GTCCGTGAGA GGATCTGGCA AAACAGTGAT 
TTCAATTTCG ACAATGTCCT TTCGGCTATG ATGGCGCTCT TCACGGTCTC GACTTTTGAG 
GGCTGGCCCG CGTTGCTGTA CAAAGCTATC GACTCAAACG GAGAGAACGT TGGTCCTGTC 
TACAACTACC GTGTGGAGAT CTCCATCTTC TTCATTATCT ACATCATCAT CGTGGCCTTC 
TTCATGATGA ATATCTTCGT GGGCTTCGTC ATCGTCACCT TCCAGGAGCA GGGAGAAAAG 
GAGTATAAGA ACTGTGAGCT GGACAAAAAT CAGCGTCAGT GTGTGGAATA TGCCTTGAAG 
GCCCGCCCCT TAAGGAGATA CATCCCCAAA AACCCATACC AGTACAAGTT CTGGTACGTG 
GTGAACTCCT CGCCTTTCGA ATATATGATG TTTGTCCTCA TCATGCTCAA CACGCTCTGC 
CTGGCCATGC AGCACTATGA GCAATCCAAG ATGTTCAATG ACGCCATGGA CATTCTGAAC 
ATGGTCTTCA CGGGGGTCTT CACCGTTGAG ATGGTTTTGA AAGTCATCGC ATTTAAGCCC 
AAGGGGTATT TTAGTGACGC CTGGAACACG TTTGACTCCC TCATCGTAAT CGGCAGCATT 
ATAGACGTGG CACTCAGCGA AGCTGACAAC TCTGAAGAGA GCAATAGAAT CTCCATCACC 
TTTTTCCGTC TTTTCCGAGT GATGCGGTTG GTGAAGCTTC TCAGCAGAGG GGAAGGCATC 
CGGACTCTGC TATGGACCTT CATTAAGTCC TTCCAGGCAC TCCCATATGT CGCCCTCCTC 
ATTGCCATGC TCTTCTTCAT CTACGCCGTC ATTGGCATGC AGATGTTTGG GAAGGTTGCC 
ATGAGAGATA ACAACCAGAT CAATAGGAAC AACAACTTCC AGACGTTTCC CCAGGCAGTG 
CTGCTGCTCT TCAGGTGTGC AACAGGGGAG GCCTGGCAGG AGATCATGCT CGCCTGCCTC 
CCTGGGAAGC TGTGTGACCC GGACTCAGAT TACAACCCAG GAGAGGAATA TACTTGTGGG 
AGCAACTTTG CCATTGTCTA CTTCATCAGC TTTTACATGC TCTGTGCGTT CCTGATCATC 
AACCTCTTCG TGGCTGTCAT CATGGACAAT TTTGACTATC TGACGAGGGA CTGGTCTATT 
CTGGGGCCTC ACCACTTGGA CGAATTCAAA AGAATATGGT CTGAATATGA CCCCGAGGCA 
AAGGGAAGGA TAAAACACCT TGATGTGGTC ACTTTGCTCC GACGTATCCA GCCTCCCCTG 
GGATTTGGAA AGTTGTGTCC ACACCGAGTG GCGTGTAAGA GATTGGTCGC CATGAACATG 
CCTCTCAACA GTGATGGGAC AGTCATGTTC AATGCAACTC TGTTTGCTTT GGTACGGACG 
GCTCTCAAGA TCAAGACTGA AGGCAACCTG GAGCAAGCTA ATGAAGAGCT CCGCGCTGTG 
ATAAAGAAAA TCTGGAAGAA GACAAGCATG AAGCTACTTG ACCAAGTTGT CCCTCCAGCT 
GGTGATGATG AGGTAACCGT GGGGAAGTTC TATGCCACTT TCCTGATACA GGACTACTTT 
AGGAAATTCA AGAAGCGGAA AGAGCAAGGC CTGGTGGGGA AATACCCTGC GAAGAACACC 
ACGATCGCCC TACAGGCGGG ATTAAGGACC CTGCATGACA TTGGGCCAGA AATCCGACGG 
GCTATATCCT GTGACTTGCA AGATGACGAA CCAGAAGACT CCAAACCAGA AGAGGAAGAT 
GTATTCAAAA GAAATGGTGC CCTGCTTGGA AACTATGTCA ATCATGTTAA TAGTGATAGG 
AGAGAGTCCC TTCAGCAGAC CAATACCACC CACCGTCCCC TGCATGTCCA AAGGCCTTCA 
ATTCCACCTG CAAGTGATAC TGAGAAACCG CTGTTTCCTC CAGCAGGAAA TTCGGTGTGT 
CATAACCATC ATAACCATAA TTCCATAGGG AAGCAAGTTC CCACCTCCAC AAATGCCAAT 
CTCAATAACG CCAATATGTC CAAAGCTGCC CACGGAAAGC GGCCCAGCAT TGGGGACCTT 
GAGCATGTGT CTGAAAACGG CCATTACTCC TACAAGCACG ACCGAGAGCT TCAAAGAAGG 
TCCAGTATTA AAAGAACCCG CTATTATGAA ACTTACATTA GGTCTGAGTC AGGAGACGAG 
CAGCTTCCAA CCATTTTCCG TGAAGACCCT GAGATACACG GCTACTTCCG AGACCCCCGC 
TGCTTCGGGG AGCAGGAATA TTTCAGTAGC GAGGAGTGCT GTGAGGATGA CAGTTCTCCC 
ACCTGGAGCA GGCAAAACTA CAGCTATTAC AACAGGTACC CAGGCAGCTC CATGGACTTT 
GAGAGGCCCC GAGGCTACCA TCACCCCCAA GGTTTCTTAG AAGATGACGA CTCTCCCATT 
GGCTATGACT CACGGAGATC TCCAAGAAGA CGCCTTCTAC CTCCTACCCC ACCATCCCAT 
CGGAGGTCCT CCTTCAACTT TGAGTGTCTG CGCAGGCAAA ACAGCCAAGA TGACGTCCTT 
CCGTCGCCTG CCCTGCCTCA CCGCGCTGCC CTGCCCCTGC ACCTTATGCA GCAGCAGATC 
ATGGCCGTTG CAGGCCTAGA TTCCAGTAAA GCCCAGAAGT ACTCGCCGAG CCACTCTACC 
CGGTCATGGG CCACCCCTCC AGCAACCCCT CCGTACCGAG ACTGGACCCC ATGCTACACC 
CCTTTGATCC AGGTGGATCG CTCAGAGTCT ATGGACCAGG TCAATGGCAG CCTGCCGTCG 
CTACACCGCA GTTCCTGGTA TACAGATGAA CCCGACATCT CCTATAGGAC TTTCACACCG 
GCCAGCCTGA CCGTCCCCAG CAGCTTCCGG AACAAAAACA GCGACAAGCA GAGGAGTGCA 
GACAGCCTGG TGGAAGCAGT CCTGATATCC GAAGGCTTGG GACGCTACGC AAGGGACCCA 
AAATTTGTCT CAGCAACAAA ACATGAGATC GCCGATGCCT GTGACCTAAC CATAGACGAG 
ATGGAGAGCG CGGCCAGCAC CCTGCTCAAT GGAAGCGTGT GTCCTCGAGC CAACGGGGAC 
ATGGGCCCCA TCTCACACCG CCAGGACTAT GAGCTCCAGG ACTTTGGTCC TGGCTACAGT 
GACGAGGAAC CAGACCCTGG GCGAGAGGAA GAGGACCTGG CAGATGAAAT GATTTGCATC 
ACCACCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_006252590.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006252652.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X7, mRNA.

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CloneID	ORa37028
Clone ID Related Accession (Same CDS sequence)	XM_006252590.3
Accession Version	XM_006252590.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006252590.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CCATCTGACA GTGAGAATAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAGCTTCTC AGCAGAGGGG AAGGCATCCG GACTCTGCTA 
TGGACCTTCA TTAAGTCCTT CCAGGCACTC CCATATGTCG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCCGTCAT TGGCATGCAG ATGTTTGGGA AGGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACGTTTCCCC AGGCAGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGGGAGGC CTGGCAGGAG ATCATGCTCG CCTGCCTCCC TGGGAAGCTG 
TGTGACCCGG ACTCAGATTA CAACCCAGGA GAGGAATATA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCGTTCC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACGAGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCT GAATATGACC CCGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TTTGCTCCGA CGTATCCAGC CTCCCCTGGG ATTTGGAAAG 
TTGTGTCCAC ACCGAGTGGC GTGTAAGAGA TTGGTCGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACTCTG TTTGCTTTGG TACGGACGGC TCTCAAGATC 
AAGACTGAAG GCAACCTGGA GCAAGCTAAT GAAGAGCTCC GCGCTGTGAT AAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAGCGGAAAG AGCAAGGCCT GGTGGGGAAA TACCCTGCGA AGAACACCAC GATCGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAACC AGAAGACTCC AAACCAGAAG AGGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CTATGTCAAT CATGTTAATA GTGATAGGAG AGAGTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT TCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGTGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGTTCCC ACCTCCACAA ATGCCAATCT CAATAACGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCGG CCCAGCATTG GGGACCTTGA GCATGTGTCT 
GAAAACGGCC ATTACTCCTA CAAGCACGAC CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTATGAAAC TTACATTAGG TCTGAGTCAG GAGACGAGCA GCTTCCAACC 
ATTTGCCGTG AAGACCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTCGGGGAG 
CAGGAATATT TCAGTAGCGA GGAGTGCTGT GAGGATGACA GTTCTCCCAC CTGGAGCAGG 
CAAAACTACA GCTATTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCATC ACCCCCAAGG TTTCTTAGAA GATGACGACT CTCCCATTGG CTATGACTCA 
CGGAGATCTC CAAGAAGACG CCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAAC AGCCAAGATG ACGTCCTTCC GTCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTTATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCGCCGAGCC ACTCTACCCG GTCATGGGCC 
ACCCCTCCAG CAACCCCTCC GTACCGAGAC TGGACCCCAT GCTACACCCC TTTGATCCAG 
GTGGATCGCT CAGAGTCTAT GGACCAGGTC AATGGCAGCC TGCCGTCGCT ACACCGCAGT 
TCCTGGTATA CAGATGAACC CGACATCTCC TATAGGACTT TCACACCGGC CAGCCTGACC 
GTCCCCAGCA GCTTCCGGAA CAAAAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAAGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCAA GGGACCCAAA ATTTGTCTCA 
GCAACAAAAC ATGAGATCGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAGAGCGCG 
GCCAGCACCC TGCTCAATGG AAGCGTGTGT CCTCGAGCCA ACGGGGACAT GGGCCCCATC 
TCACACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAACCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAAATGA TTTGCATCAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006252652.1
CDS	1..6540
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPSDSENIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_006252590.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006252590.3

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CCATCTGACA GTGAGAATAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAGCTTCTC AGCAGAGGGG AAGGCATCCG GACTCTGCTA 
TGGACCTTCA TTAAGTCCTT CCAGGCACTC CCATATGTCG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCCGTCAT TGGCATGCAG ATGTTTGGGA AGGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACGTTTCCCC AGGCAGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGGGAGGC CTGGCAGGAG ATCATGCTCG CCTGCCTCCC TGGGAAGCTG 
TGTGACCCGG ACTCAGATTA CAACCCAGGA GAGGAATATA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCGTTCC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACGAGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCT GAATATGACC CCGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TTTGCTCCGA CGTATCCAGC CTCCCCTGGG ATTTGGAAAG 
TTGTGTCCAC ACCGAGTGGC GTGTAAGAGA TTGGTCGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACTCTG TTTGCTTTGG TACGGACGGC TCTCAAGATC 
AAGACTGAAG GCAACCTGGA GCAAGCTAAT GAAGAGCTCC GCGCTGTGAT AAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAGCGGAAAG AGCAAGGCCT GGTGGGGAAA TACCCTGCGA AGAACACCAC GATCGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAACC AGAAGACTCC AAACCAGAAG AGGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CTATGTCAAT CATGTTAATA GTGATAGGAG AGAGTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT TCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGTGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGTTCCC ACCTCCACAA ATGCCAATCT CAATAACGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCGG CCCAGCATTG GGGACCTTGA GCATGTGTCT 
GAAAACGGCC ATTACTCCTA CAAGCACGAC CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTATGAAAC TTACATTAGG TCTGAGTCAG GAGACGAGCA GCTTCCAACC 
ATTTGCCGTG AAGACCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTCGGGGAG 
CAGGAATATT TCAGTAGCGA GGAGTGCTGT GAGGATGACA GTTCTCCCAC CTGGAGCAGG 
CAAAACTACA GCTATTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCATC ACCCCCAAGG TTTCTTAGAA GATGACGACT CTCCCATTGG CTATGACTCA 
CGGAGATCTC CAAGAAGACG CCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAAC AGCCAAGATG ACGTCCTTCC GTCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTTATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCGCCGAGCC ACTCTACCCG GTCATGGGCC 
ACCCCTCCAG CAACCCCTCC GTACCGAGAC TGGACCCCAT GCTACACCCC TTTGATCCAG 
GTGGATCGCT CAGAGTCTAT GGACCAGGTC AATGGCAGCC TGCCGTCGCT ACACCGCAGT 
TCCTGGTATA CAGATGAACC CGACATCTCC TATAGGACTT TCACACCGGC CAGCCTGACC 
GTCCCCAGCA GCTTCCGGAA CAAAAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAAGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCAA GGGACCCAAA ATTTGTCTCA 
GCAACAAAAC ATGAGATCGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAGAGCGCG 
GCCAGCACCC TGCTCAATGG AAGCGTGTGT CCTCGAGCCA ACGGGGACAT GGGCCCCATC 
TCACACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAACCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAAATGA TTTGCATCAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_006252594.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006252656.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X12, mRNA.

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CloneID	ORa37032
Clone ID Related Accession (Same CDS sequence)	XM_006252594.3
Accession Version	XM_006252594.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006252594.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTGCTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CCATCTGACA GTGAGAATAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAGCTTCTC AGCAGAGGGG AAGGCATCCG GACTCTGCTA 
TGGACCTTCA TTAAGTCCTT CCAGGCACTC CCATATGTCG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCCGTCAT TGGCATGCAG ATGTTTGGGA AGGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACGTTTCCCC AGGCAGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGGGAGGC CTGGCAGGAG ATCATGCTCG CCTGCCTCCC TGGGAAGCTG 
TGTGACCCGG ACTCAGATTA CAACCCAGGA GAGGAATATA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCGTTCC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACGAGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCT GAATATGACC CCGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TTTGCTCCGA CGTATCCAGC CTCCCCTGGG ATTTGGAAAG 
TTGTGTCCAC ACCGAGTGGC GTGTAAGAGA TTGGTCGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACTCTG TTTGCTTTGG TACGGACGGC TCTCAAGATC 
AAGACTGAAG GCAACCTGGA GCAAGCTAAT GAAGAGCTCC GCGCTGTGAT AAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAGCGGAAAG AGCAAGGCCT GGTGGGGAAA TACCCTGCGA AGAACACCAC GATCGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAACC AGAAGACTCC AAACCAGAAG AGGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CTATGTCAAT CATGTTAATA GTGATAGGAG AGAGTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT TCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGTGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGTTCCC ACCTCCACAA ATGCCAATCT CAATAACGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCGG CCCAGCATTG GGGACCTTGA GCATGTGTCT 
GAAAACGGCC ATTACTCCTA CAAGCACGAC CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTATGAAAC TTACATTAGG TCTGAGTCAG GAGACGAGCA GCTTCCAACC 
ATTTGCCGTG AAGACCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTCGGGGAG 
CAGGAATATT TCAGTAGCGA GGAGTGCTGT GAGGATGACA GTTCTCCCAC CTGGAGCAGG 
CAAAACTACA GCTATTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCATC ACCCCCAAGG TTTCTTAGAA GATGACGACT CTCCCATTGG CTATGACTCA 
CGGAGATCTC CAAGAAGACG CCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAAC AGCCAAGATG ACGTCCTTCC GTCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTTATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCGCCGAGCC ACTCTACCCG GTCATGGGCC 
ACCCCTCCAG CAACCCCTCC GTACCGAGAC TGGACCCCAT GCTACACCCC TTTGATCCAG 
GTGGATCGCT CAGAGTCTAT GGACCAGGTC AATGGCAGCC TGCCGTCGCT ACACCGCAGT 
TCCTGGTATA CAGATGAACC CGACATCTCC TATAGGACTT TCACACCGGC CAGCCTGACC 
GTCCCCAGCA GCTTCCGGAA CAAAAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAAGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCAA GGGACCCAAA ATTTGTCTCA 
GCAACAAAAC ATGAGATCGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAGAGCGCG 
GCCAGCACCC TGCTCAATGG AAGCGTGTGT CCTCGAGCCA ACGGGGACAT GGGCCCCATC 
TCACACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAACCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAAATGA TTTGCATCAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006252656.1
CDS	1..6480
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPSDSENIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_006252594.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006252594.3

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTGCTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC CCATCTGACA GTGAGAATAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAGCTTCTC AGCAGAGGGG AAGGCATCCG GACTCTGCTA 
TGGACCTTCA TTAAGTCCTT CCAGGCACTC CCATATGTCG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCCGTCAT TGGCATGCAG ATGTTTGGGA AGGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACGTTTCCCC AGGCAGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGGGAGGC CTGGCAGGAG ATCATGCTCG CCTGCCTCCC TGGGAAGCTG 
TGTGACCCGG ACTCAGATTA CAACCCAGGA GAGGAATATA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCGTTCC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACGAGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCT GAATATGACC CCGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TTTGCTCCGA CGTATCCAGC CTCCCCTGGG ATTTGGAAAG 
TTGTGTCCAC ACCGAGTGGC GTGTAAGAGA TTGGTCGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACTCTG TTTGCTTTGG TACGGACGGC TCTCAAGATC 
AAGACTGAAG GCAACCTGGA GCAAGCTAAT GAAGAGCTCC GCGCTGTGAT AAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAGCGGAAAG AGCAAGGCCT GGTGGGGAAA TACCCTGCGA AGAACACCAC GATCGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAACC AGAAGACTCC AAACCAGAAG AGGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CTATGTCAAT CATGTTAATA GTGATAGGAG AGAGTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT TCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGTGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGTTCCC ACCTCCACAA ATGCCAATCT CAATAACGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCGG CCCAGCATTG GGGACCTTGA GCATGTGTCT 
GAAAACGGCC ATTACTCCTA CAAGCACGAC CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTATGAAAC TTACATTAGG TCTGAGTCAG GAGACGAGCA GCTTCCAACC 
ATTTGCCGTG AAGACCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTCGGGGAG 
CAGGAATATT TCAGTAGCGA GGAGTGCTGT GAGGATGACA GTTCTCCCAC CTGGAGCAGG 
CAAAACTACA GCTATTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCATC ACCCCCAAGG TTTCTTAGAA GATGACGACT CTCCCATTGG CTATGACTCA 
CGGAGATCTC CAAGAAGACG CCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAAC AGCCAAGATG ACGTCCTTCC GTCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTTATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCGCCGAGCC ACTCTACCCG GTCATGGGCC 
ACCCCTCCAG CAACCCCTCC GTACCGAGAC TGGACCCCAT GCTACACCCC TTTGATCCAG 
GTGGATCGCT CAGAGTCTAT GGACCAGGTC AATGGCAGCC TGCCGTCGCT ACACCGCAGT 
TCCTGGTATA CAGATGAACC CGACATCTCC TATAGGACTT TCACACCGGC CAGCCTGACC 
GTCCCCAGCA GCTTCCGGAA CAAAAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAAGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCAA GGGACCCAAA ATTTGTCTCA 
GCAACAAAAC ATGAGATCGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAGAGCGCG 
GCCAGCACCC TGCTCAATGG AAGCGTGTGT CCTCGAGCCA ACGGGGACAT GGGCCCCATC 
TCACACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAACCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAAATGA TTTGCATCAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_006252595.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006252657.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X13, mRNA.

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CloneID	ORa37033
Clone ID Related Accession (Same CDS sequence)	XM_006252595.3
Accession Version	XM_006252595.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006252595.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAGC TTCTCAGCAG AGGGGAAGGC 
ATCCGGACTC TGCTATGGAC CTTCATTAAG TCCTTCCAGG CACTCCCATA TGTCGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCC GTCATTGGCA TGCAGATGTT TGGGAAGGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACGTT TCCCCAGGCA 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGG GAGGCCTGGC AGGAGATCAT GCTCGCCTGC 
CTCCCTGGGA AGCTGTGTGA CCCGGACTCA GATTACAACC CAGGAGAGGA ATATACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC GTTCCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACGAG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCTGAATA TGACCCCGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTTTGC TCCGACGTAT CCAGCCTCCC 
CTGGGATTTG GAAAGTTGTG TCCACACCGA GTGGCGTGTA AGAGATTGGT CGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CTCTGTTTGC TTTGGTACGG 
ACGGCTCTCA AGATCAAGAC TGAAGGCAAC CTGGAGCAAG CTAATGAAGA GCTCCGCGCT 
GTGATAAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAGCG GAAAGAGCAA GGCCTGGTGG GGAAATACCC TGCGAAGAAC 
ACCACGATCG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAACCAGAAG ACTCCAAACC AGAAGAGGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACTATG TCAATCATGT TAATAGTGAT 
AGGAGAGAGT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATTCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGTG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG TTCCCACCTC CACAAATGCC 
AATCTCAATA ACGCCAATAT GTCCAAAGCT GCCCACGGAA AGCGGCCCAG CATTGGGGAC 
CTTGAGCATG TGTCTGAAAA CGGCCATTAC TCCTACAAGC ACGACCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAT GAAACTTACA TTAGGTCTGA GTCAGGAGAC 
GAGCAGCTTC CAACCATTTG CCGTGAAGAC CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTCG GGGAGCAGGA ATATTTCAGT AGCGAGGAGT GCTGTGAGGA TGACAGTTCT 
CCCACCTGGA GCAGGCAAAA CTACAGCTAT TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCATCACCCC CAAGGTTTCT TAGAAGATGA CGACTCTCCC 
ATTGGCTATG ACTCACGGAG ATCTCCAAGA AGACGCCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAACAGCCA AGATGACGTC 
CTTCCGTCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTTAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCGCC GAGCCACTCT 
ACCCGGTCAT GGGCCACCCC TCCAGCAACC CCTCCGTACC GAGACTGGAC CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA TCGCTCAGAG TCTATGGACC AGGTCAATGG CAGCCTGCCG 
TCGCTACACC GCAGTTCCTG GTATACAGAT GAACCCGACA TCTCCTATAG GACTTTCACA 
CCGGCCAGCC TGACCGTCCC CAGCAGCTTC CGGAACAAAA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAAGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCAAGGGAC 
CCAAAATTTG TCTCAGCAAC AAAACATGAG ATCGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAGA GCGCGGCCAG CACCCTGCTC AATGGAAGCG TGTGTCCTCG AGCCAACGGG 
GACATGGGCC CCATCTCACA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AACCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA AATGATTTGC 
ATCACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006252657.1
CDS	1..6435
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRTRYYETYIRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_006252595.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006252595.3

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAG 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAGC TTCTCAGCAG AGGGGAAGGC 
ATCCGGACTC TGCTATGGAC CTTCATTAAG TCCTTCCAGG CACTCCCATA TGTCGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCC GTCATTGGCA TGCAGATGTT TGGGAAGGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACGTT TCCCCAGGCA 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGG GAGGCCTGGC AGGAGATCAT GCTCGCCTGC 
CTCCCTGGGA AGCTGTGTGA CCCGGACTCA GATTACAACC CAGGAGAGGA ATATACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC GTTCCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACGAG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCTGAATA TGACCCCGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTTTGC TCCGACGTAT CCAGCCTCCC 
CTGGGATTTG GAAAGTTGTG TCCACACCGA GTGGCGTGTA AGAGATTGGT CGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CTCTGTTTGC TTTGGTACGG 
ACGGCTCTCA AGATCAAGAC TGAAGGCAAC CTGGAGCAAG CTAATGAAGA GCTCCGCGCT 
GTGATAAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAGCG GAAAGAGCAA GGCCTGGTGG GGAAATACCC TGCGAAGAAC 
ACCACGATCG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAACCAGAAG ACTCCAAACC AGAAGAGGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACTATG TCAATCATGT TAATAGTGAT 
AGGAGAGAGT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATTCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGTG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG TTCCCACCTC CACAAATGCC 
AATCTCAATA ACGCCAATAT GTCCAAAGCT GCCCACGGAA AGCGGCCCAG CATTGGGGAC 
CTTGAGCATG TGTCTGAAAA CGGCCATTAC TCCTACAAGC ACGACCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAT GAAACTTACA TTAGGTCTGA GTCAGGAGAC 
GAGCAGCTTC CAACCATTTG CCGTGAAGAC CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTCG GGGAGCAGGA ATATTTCAGT AGCGAGGAGT GCTGTGAGGA TGACAGTTCT 
CCCACCTGGA GCAGGCAAAA CTACAGCTAT TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCATCACCCC CAAGGTTTCT TAGAAGATGA CGACTCTCCC 
ATTGGCTATG ACTCACGGAG ATCTCCAAGA AGACGCCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAACAGCCA AGATGACGTC 
CTTCCGTCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTTAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCGCC GAGCCACTCT 
ACCCGGTCAT GGGCCACCCC TCCAGCAACC CCTCCGTACC GAGACTGGAC CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA TCGCTCAGAG TCTATGGACC AGGTCAATGG CAGCCTGCCG 
TCGCTACACC GCAGTTCCTG GTATACAGAT GAACCCGACA TCTCCTATAG GACTTTCACA 
CCGGCCAGCC TGACCGTCCC CAGCAGCTTC CGGAACAAAA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAAGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCAAGGGAC 
CCAAAATTTG TCTCAGCAAC AAAACATGAG ATCGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAGA GCGCGGCCAG CACCCTGCTC AATGGAAGCG TGTGTCCTCG AGCCAACGGG 
GACATGGGCC CCATCTCACA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AACCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA AATGATTTGC 
ATCACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_006252597.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006252659.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X15, mRNA.

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CloneID	ORa37035
Clone ID Related Accession (Same CDS sequence)	XM_006252597.3
Accession Version	XM_006252597.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006252597.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTGCTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAGC TTCTCAGCAG AGGGGAAGGC 
ATCCGGACTC TGCTATGGAC CTTCATTAAG TCCTTCCAGG CACTCCCATA TGTCGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCC GTCATTGGCA TGCAGATGTT TGGGAAGGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACGTT TCCCCAGGCA 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGG GAGGCCTGGC AGGAGATCAT GCTCGCCTGC 
CTCCCTGGGA AGCTGTGTGA CCCGGACTCA GATTACAACC CAGGAGAGGA ATATACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC GTTCCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACGAG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCTGAATA TGACCCCGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTTTGC TCCGACGTAT CCAGCCTCCC 
CTGGGATTTG GAAAGTTGTG TCCACACCGA GTGGCGTGTA AGAGATTGGT CGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CTCTGTTTGC TTTGGTACGG 
ACGGCTCTCA AGATCAAGAC TGAAGGCAAC CTGGAGCAAG CTAATGAAGA GCTCCGCGCT 
GTGATAAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAGCG GAAAGAGCAA GGCCTGGTGG GGAAATACCC TGCGAAGAAC 
ACCACGATCG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAACCAGAAG ACTCCAAACC AGAAGAGGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACTATG TCAATCATGT TAATAGTGAT 
AGGAGAGAGT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATTCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGTG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG TTCCCACCTC CACAAATGCC 
AATCTCAATA ACGCCAATAT GTCCAAAGCT GCCCACGGAA AGCGGCCCAG CATTGGGGAC 
CTTGAGCATG TGTCTGAAAA CGGCCATTAC TCCTACAAGC ACGACCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGGTC TGAGTCAGGA GACGAGCAGC TTCCAACCAT TTGCCGTGAA 
GACCCTGAGA TACACGGCTA CTTCCGAGAC CCCCGCTGCT TCGGGGAGCA GGAATATTTC 
AGTAGCGAGG AGTGCTGTGA GGATGACAGT TCTCCCACCT GGAGCAGGCA AAACTACAGC 
TATTACAACA GGTACCCAGG CAGCTCCATG GACTTTGAGA GGCCCCGAGG CTACCATCAC 
CCCCAAGGTT TCTTAGAAGA TGACGACTCT CCCATTGGCT ATGACTCACG GAGATCTCCA 
AGAAGACGCC TTCTACCTCC TACCCCACCA TCCCATCGGA GGTCCTCCTT CAACTTTGAG 
TGTCTGCGCA GGCAAAACAG CCAAGATGAC GTCCTTCCGT CGCCTGCCCT GCCTCACCGC 
GCTGCCCTGC CCCTGCACCT TATGCAGCAG CAGATCATGG CCGTTGCAGG CCTAGATTCC 
AGTAAAGCCC AGAAGTACTC GCCGAGCCAC TCTACCCGGT CATGGGCCAC CCCTCCAGCA 
ACCCCTCCGT ACCGAGACTG GACCCCATGC TACACCCCTT TGATCCAGGT GGATCGCTCA 
GAGTCTATGG ACCAGGTCAA TGGCAGCCTG CCGTCGCTAC ACCGCAGTTC CTGGTATACA 
GATGAACCCG ACATCTCCTA TAGGACTTTC ACACCGGCCA GCCTGACCGT CCCCAGCAGC 
TTCCGGAACA AAAACAGCGA CAAGCAGAGG AGTGCAGACA GCCTGGTGGA AGCAGTCCTG 
ATATCCGAAG GCTTGGGACG CTACGCAAGG GACCCAAAAT TTGTCTCAGC AACAAAACAT 
GAGATCGCCG ATGCCTGTGA CCTAACCATA GACGAGATGG AGAGCGCGGC CAGCACCCTG 
CTCAATGGAA GCGTGTGTCC TCGAGCCAAC GGGGACATGG GCCCCATCTC ACACCGCCAG 
GACTATGAGC TCCAGGACTT TGGTCCTGGC TACAGTGACG AGGAACCAGA CCCTGGGCGA 
GAGGAAGAGG ACCTGGCAGA TGAAATGATT TGCATCACCA CCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006252659.1
CDS	1..6408
Translation	MMMMMMMKKMQHQRQQQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEEAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNYVNHVNSDRRESLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGDLEHVSENGHYSYKHDRELQRRSSIKRSESGDEQLPTICREDPEIHGYFRDPRCFGEQEYFSSEECCEDDSSPTWSRQNYSYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPIGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQNSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_006252597.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 D (Cacna1d), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006252597.3

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGAGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ACAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT TGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACGTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTCTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG ATTGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCGTTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTACG GACTGCTGCT GCATCCTAAT GCTTACGTTA GGAACGGATG GAACTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC GCTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTCCACA TAGCCCTTTT GGTGTTATTC 
GTAATTATCA TCTATGCGAT TATAGGGTTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCATG CGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAATGTA GGAGCGGCTG GGTCGGCCCC 
AATGGAGGCA TCACTAATTT TGACAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTGCTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCACGTGGA 
GATTTCCAGA AGCTTCGGGA GAAGCAGCAG CTGGAAGAGG ATCTCAAGGG CTACTTGGAC 
TGGATCACCC AGGCAGAAGA CATAGATCCT GAGAATGAAG AGGAGGGTGG AGAGGAGGGC 
AAACGAAACA CTAGCATGCC CACCAGTGAG ACCGAATCTG TGAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA AGTCTCTGCC AAGCCATCTC AAAATCCAAG 
CTCAGCCGTC GGTGGCGTCG CTGGAACCGG TTCAATCGCA GGAGATGCAG GGCAGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATC GTTCTGGTGT TTCTCAACAC TCTGACGATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACGCAGA TTCAAGATAT AGCGAACAAA 
GTGCTCTTGG CTTTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCCTACTTCG TCTCTCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGAATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GCGTCTCTGT GTTCCGGTGT 
GTACGTCTCC TGAGGATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTGGTG 
GCGTCCCTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTGCTGCT CTTCCTCTTC 
ATCATCATCT TCTCCCTGCT GGGAATGCAG CTGTTCGGAG GGAAGTTCAA TTTCGATGAG 
ACACAGACCA AGCGAAGCAC CTTCGACAAC TTCCCACAGG CGCTCCTGAC TGTGTTCCAG 
ATCCTGACGG GCGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTACGGAGGC 
CCGTCCTCCT CTGGAATGAT TGTCTGTATT TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAACGTCTT CTTGGCCATT GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCCGAA GAAAAAGAAA GGAAAAAAAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA AAACAACAAA CCAGAGGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CTATTGATGA CTATCAAGAG GAGGCTGAAG ATAAGGACCC TTATCCACCC 
TGCGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGACGAGCC TGAGGTTCCT 
GCTGGTCCCC GTCCTCGAAG AATCTCAGAG TTGAACATGA AGGAGAAAAT TGCACCCATC 
CCCGAAGGAA GCGCATTCTT CATTCTTAGC AAGACCAACC CGATCCGTGT GGGTTGCCAC 
AAACTCATCA ACCACCACAT CTTCACCAAC CTCATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA TCCCATCCGC AGCCACTCCT TCCGCAACAC TATACTGGGC 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCGT TCTTGCACAA AGGGGCTTTC TGTAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTCGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCGGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCTTGAGG CCTCTCAGAG CAATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATCGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCT TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA GGCCAAAAGT AACCCCGAGG AGTGCAGGGG GCTTTTCATC 
CTTTATAAGG ACGGCGATGT CGACAGTCCC GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAATT TCGACAATGT CCTTTCGGCT ATGATGGCGC TCTTCACGGT CTCGACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATCGACTCAA ACGGAGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATTA TCTACATCAT CATCGTGGCC 
TTCTTCATGA TGAATATCTT CGTGGGCTTC GTCATCGTCA CCTTCCAGGA GCAGGGAGAA 
AAGGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTGGA ATATGCCTTG 
AAGGCCCGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATATATG ATGTTTGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAATCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACGGGGGT CTTCACCGTT GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCACTCAG CGAAGCTGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAGC TTCTCAGCAG AGGGGAAGGC 
ATCCGGACTC TGCTATGGAC CTTCATTAAG TCCTTCCAGG CACTCCCATA TGTCGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCC GTCATTGGCA TGCAGATGTT TGGGAAGGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACGTT TCCCCAGGCA 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGG GAGGCCTGGC AGGAGATCAT GCTCGCCTGC 
CTCCCTGGGA AGCTGTGTGA CCCGGACTCA GATTACAACC CAGGAGAGGA ATATACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC GTTCCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACGAG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCTGAATA TGACCCCGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTTTGC TCCGACGTAT CCAGCCTCCC 
CTGGGATTTG GAAAGTTGTG TCCACACCGA GTGGCGTGTA AGAGATTGGT CGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CTCTGTTTGC TTTGGTACGG 
ACGGCTCTCA AGATCAAGAC TGAAGGCAAC CTGGAGCAAG CTAATGAAGA GCTCCGCGCT 
GTGATAAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAGCG GAAAGAGCAA GGCCTGGTGG GGAAATACCC TGCGAAGAAC 
ACCACGATCG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAACCAGAAG ACTCCAAACC AGAAGAGGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACTATG TCAATCATGT TAATAGTGAT 
AGGAGAGAGT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATTCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGTG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG TTCCCACCTC CACAAATGCC 
AATCTCAATA ACGCCAATAT GTCCAAAGCT GCCCACGGAA AGCGGCCCAG CATTGGGGAC 
CTTGAGCATG TGTCTGAAAA CGGCCATTAC TCCTACAAGC ACGACCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGGTC TGAGTCAGGA GACGAGCAGC TTCCAACCAT TTGCCGTGAA 
GACCCTGAGA TACACGGCTA CTTCCGAGAC CCCCGCTGCT TCGGGGAGCA GGAATATTTC 
AGTAGCGAGG AGTGCTGTGA GGATGACAGT TCTCCCACCT GGAGCAGGCA AAACTACAGC 
TATTACAACA GGTACCCAGG CAGCTCCATG GACTTTGAGA GGCCCCGAGG CTACCATCAC 
CCCCAAGGTT TCTTAGAAGA TGACGACTCT CCCATTGGCT ATGACTCACG GAGATCTCCA 
AGAAGACGCC TTCTACCTCC TACCCCACCA TCCCATCGGA GGTCCTCCTT CAACTTTGAG 
TGTCTGCGCA GGCAAAACAG CCAAGATGAC GTCCTTCCGT CGCCTGCCCT GCCTCACCGC 
GCTGCCCTGC CCCTGCACCT TATGCAGCAG CAGATCATGG CCGTTGCAGG CCTAGATTCC 
AGTAAAGCCC AGAAGTACTC GCCGAGCCAC TCTACCCGGT CATGGGCCAC CCCTCCAGCA 
ACCCCTCCGT ACCGAGACTG GACCCCATGC TACACCCCTT TGATCCAGGT GGATCGCTCA 
GAGTCTATGG ACCAGGTCAA TGGCAGCCTG CCGTCGCTAC ACCGCAGTTC CTGGTATACA 
GATGAACCCG ACATCTCCTA TAGGACTTTC ACACCGGCCA GCCTGACCGT CCCCAGCAGC 
TTCCGGAACA AAAACAGCGA CAAGCAGAGG AGTGCAGACA GCCTGGTGGA AGCAGTCCTG 
ATATCCGAAG GCTTGGGACG CTACGCAAGG GACCCAAAAT TTGTCTCAGC AACAAAACAT 
GAGATCGCCG ATGCCTGTGA CCTAACCATA GACGAGATGG AGAGCGCGGC CAGCACCCTG 
CTCAATGGAA GCGTGTGTCC TCGAGCCAAC GGGGACATGG GCCCCATCTC ACACCGCCAG 
GACTATGAGC TCCAGGACTT TGGTCCTGGC TACAGTGACG AGGAACCAGA CCCTGGGCGA 
GAGGAAGAGG ACCTGGCAGA TGAAATGATT TGCATCACCA CCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Cacna1d
Entrez Gene ID	29716
Full Name	calcium voltage-gated channel subunit alpha1 D
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 D Names voltage-dependent L-type calcium channel subunit alpha-1D CaV1.3alpha1 RBD brain class D calcium channel alpha-1 subunit calcium channel, L type, alpha-1 polypeptide calcium channel, voltage-dependent, L type, alpha 1D subunit voltage-dependent calcium channel subunit alpha1D voltage-gated calcium channel pore forming subunit CaV1.3alpha1 IVS3-IVS4 extracellular linker voltage-gated calcium channel subunit alpha Cav1.3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p16
Summary	has high voltage L-type calcium channel activity that is sensitive to dihydropyridines [RGD, Feb 2006].

Bos taurus (cattle)	CACNA1D	NP_001179954.1
Rattus norvegicus (Norway rat)	Cacna1d	NP_058994.1
Gallus gallus (chicken)	CACNA1D	NP_990365.1
Homo sapiens (human)	CACNA1D	NP_000711.1
Pan troglodytes (chimpanzee)	CACNA1D	XP_001173076.3
	CACNA1D	XP_002802833.1
Mus musculus (house mouse)	Cacna1d	NP_001077085.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012434	XP_001231059.2
Canis lupus familiaris (dog)	CACNA1D	XP_849431.1
Danio rerio (zebrafish)	cacna1da	NP_982351.1
Drosophila melanogaster (fruit fly)	Ca-alpha1D	NP_001260484.1
Caenorhabditis elegans (roundworm)	egl-19	NP_001023079.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Cacna1d cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

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