egl-19 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	egl-19
Entrez Gene ID	177513
Full Name	Voltage-dependent L-type calcium channel subunit alpha
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_171379.4	NP_741442.1	Voltage-dependent L-type calcium channel subunit alpha
NM_001027908.4	NP_001023079.1	Voltage-dependent L-type calcium channel subunit alpha
NM_171379.3	NP_741442.1	Voltage-dependent L-type calcium channel subunit alpha
NM_001307266.1	NP_001294195.1	Voltage-dependent L-type calcium channel subunit alpha
NM_001372868.1	NP_001360343.1	Voltage-dependent L-type calcium channel subunit alpha
NM_001027908.3	NP_001023079.1	Voltage-dependent L-type calcium channel subunit alpha

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CACNA1D	NP_001179954.1
Rattus norvegicus (Norway rat)	Cacna1d	NP_058994.1
Gallus gallus (chicken)	CACNA1D	NP_990365.1
Homo sapiens (human)	CACNA1D	NP_000711.1
Pan troglodytes (chimpanzee)	CACNA1D	XP_001173076.3
	CACNA1D	XP_002802833.1
Mus musculus (house mouse)	Cacna1d	NP_001077085.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012434	XP_001231059.2
Canis lupus familiaris (dog)	CACNA1D	XP_849431.1
Danio rerio (zebrafish)	cacna1da	NP_982351.1
Drosophila melanogaster (fruit fly)	Ca-alpha1D	NP_001260484.1
Caenorhabditis elegans (roundworm)	egl-19	NP_001023079.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SLO-1 and SLO-2 localize close to UNC-2 and EGL-19 in AWC, suggesting a role of possible functional coupling between SLO BK channels and voltage-activated calcium channels in AWC asymmetry.
Title: SLO BK Potassium Channels Couple Gap Junctions to Inhibition of Calcium Signaling in Olfactory Neuron Diversification.

Hyperactivation of L-type voltage-gated Ca2+ channels in Caenorhabditis elegans striated muscle can result from point mutations in the IS6 or the IIIS4 segment of the alpha1 subunit.
Title: Hyperactivation of L-type voltage-gated Ca2+ channels in Caenorhabditis elegans striated muscle can result from point mutations in the IS6 or the IIIS4 segment of the ?1 subunit.

EGL-19, UNC-36, and CCB-1 underlie voltage-dependent calcium currents in C. elegans striated muscle.
Title: The alpha1 subunit EGL-19, the alpha2/delta subunit UNC-36, and the beta subunit CCB-1 underlie voltage-dependent calcium currents in Caenorhabditis elegans striated muscle.

central role for EGL-19 and UNC-36 in excitability and functional activity of the mechanosensory neurons
Title: Effects of voltage-gated calcium channel subunit genes on calcium influx in cultured C. elegans mechanosensory neurons.

EGL-19 activates at high voltages and inactivates slowly; thus it may maintain the plateau phase of the action potential.
Title: CCA-1, EGL-19 and EXP-2 currents shape action potentials in the Caenorhabditis elegans pharynx.

The following egl-19 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the egl-19 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa353390	NM_001307266.1 Latest version!	Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OCa353390	NM_001372868.1 Latest version!	Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OCa343324	NM_171379.4 Latest version!	Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa343325	NM_001027908.4 Latest version!	Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa343324	NM_171379.3	Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa343325	NM_001027908.3	Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

egl-19 ( NM_001307266.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294195.1 Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), partial mRNA.

Price & Availability↑

CloneID	OCa353390
Clone ID Related Accession (Same CDS sequence)	NM_001307266.1 , NM_001372868.1
Accession Version	NM_001307266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Voltage-dependent L-type calcium channel subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGCATCGTG GAGCTCCAAT CAATCAATCA ATAAATCTTC CACCTGTCAA TGGATCAGCT 
CGACGTCTTC CCGCCCTTCC GCCGTACGCA AATCATATTC ACGATGAGAC GGACGACGGC 
CCCAGGTATC GGGATACTGG CGATCGAGCT GGTTACGATC AATCAAGCAG AATGGTTGTT 
GCCAATAGAA ATCTGCCTGT TGATCCCGAT GAAGAAGAGC AATGGATGAG AAGTGGTGGA 
CCGTCAAATC GATCTGACAG ACGAAACCCA CATCTACGAG AACCGATGCT TGTTGCTCGG 
GGAGCCGCTC TTGCACTCGC TGGAATGTCA TCAGAAGCCT ACGAAGGTAC TTATAGGCCG 
GTTGGAGAAG GAAAGTCTGT TCGACTTCCA TTCTCATCTC GACCTGTTCT CCGACCTGCC 
GAAGATTCAC GACCTGTTGA TCGACTGATC GGGCAATCAT TAGGACTTGG AAGGTACGCC 
GACGCTCGGA TTGTCGGAGC CGCTCGACGA GAAATTGAGG AAGCATATTC ACTTGGTGAA 
CAGGAAATTG ACTTGGCAGC AGACTCATTA GCTCCATTAA TGCAACATGT TGGAATGCAT 
GATATCAGAG ATATCAATGA AAACTCTCGA AGCGCACTTT TACGACCCGC CGAAAATTCA 
TCTCGGCAGC ATGATTCACG CGGAGGATCA CAAGAAGATC TACTTTTAGT TACAACTCTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294195.1
CDS	1..723
Translation	MHRGAPINQSINLPPVNGSARRLPALPPYANHIHDETDDGPRYRDTGDRAGYDQSSRMVVANRNLPVDPDEEEQWMRSGGPSNRSDRRNPHLREPMLVARGAALALAGMSSEAYEGTYRPVGEGKSVRLPFSSRPVLRPAEDSRPVDRLIGQSLGLGRYADARIVGAARREIEEAYSLGEQEIDLAADSLAPLMQHVGMHDIRDINENSRSALLRPAENSSRQHDSRGGSQEDLLLVTTL

Target ORF information:

RefSeq Version	NM_001307266.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307266.1

ORF Insert Sequence:

ATGCATCGTG GAGCTCCAAT CAATCAATCA ATAAATCTTC CACCTGTCAA TGGATCAGCT 
CGACGTCTTC CCGCCCTTCC GCCGTACGCA AATCATATTC ACGATGAGAC GGACGACGGC 
CCCAGGTATC GGGATACTGG CGATCGAGCT GGTTACGATC AATCAAGCAG AATGGTTGTT 
GCCAATAGAA ATCTGCCTGT TGATCCCGAT GAAGAAGAGC AATGGATGAG AAGTGGTGGA 
CCGTCAAATC GATCTGACAG ACGAAACCCA CATCTACGAG AACCGATGCT TGTTGCTCGG 
GGAGCCGCTC TTGCACTCGC TGGAATGTCA TCAGAAGCCT ACGAAGGTAC TTATAGGCCG 
GTTGGAGAAG GAAAGTCTGT TCGACTTCCA TTCTCATCTC GACCTGTTCT CCGACCTGCC 
GAAGATTCAC GACCTGTTGA TCGACTGATC GGGCAATCAT TAGGACTTGG AAGGTACGCC 
GACGCTCGGA TTGTCGGAGC CGCTCGACGA GAAATTGAGG AAGCATATTC ACTTGGTGAA 
CAGGAAATTG ACTTGGCAGC AGACTCATTA GCTCCATTAA TGCAACATGT TGGAATGCAT 
GATATCAGAG ATATCAATGA AAACTCTCGA AGCGCACTTT TACGACCCGC CGAAAATTCA 
TCTCGGCAGC ATGATTCACG CGGAGGATCA CAAGAAGATC TACTTTTAGT TACAACTCTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-19 ( NM_001372868.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360343.1 Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), mRNA.

Price & Availability↑

CloneID	OCa353390
Clone ID Related Accession (Same CDS sequence)	NM_001307266.1 , NM_001372868.1
Accession Version	NM_001372868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Voltage-dependent L-type calcium channel subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282).

ATGCATCGTG GAGCTCCAAT CAATCAATCA ATAAATCTTC CACCTGTCAA TGGATCAGCT 
CGACGTCTTC CCGCCCTTCC GCCGTACGCA AATCATATTC ACGATGAGAC GGACGACGGC 
CCCAGGTATC GGGATACTGG CGATCGAGCT GGTTACGATC AATCAAGCAG AATGGTTGTT 
GCCAATAGAA ATCTGCCTGT TGATCCCGAT GAAGAAGAGC AATGGATGAG AAGTGGTGGA 
CCGTCAAATC GATCTGACAG ACGAAACCCA CATCTACGAG AACCGATGCT TGTTGCTCGG 
GGAGCCGCTC TTGCACTCGC TGGAATGTCA TCAGAAGCCT ACGAAGGTAC TTATAGGCCG 
GTTGGAGAAG GAAAGTCTGT TCGACTTCCA TTCTCATCTC GACCTGTTCT CCGACCTGCC 
GAAGATTCAC GACCTGTTGA TCGACTGATC GGGCAATCAT TAGGACTTGG AAGGTACGCC 
GACGCTCGGA TTGTCGGAGC CGCTCGACGA GAAATTGAGG AAGCATATTC ACTTGGTGAA 
CAGGAAATTG ACTTGGCAGC AGACTCATTA GCTCCATTAA TGCAACATGT TGGAATGCAT 
GATATCAGAG ATATCAATGA AAACTCTCGA AGCGCACTTT TACGACCCGC CGAAAATTCA 
TCTCGGCAGC ATGATTCACG CGGAGGATCA CAAGAAGATC TACTTTTAGT TACAACTCTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360343.1
CDS	4820..5542
Translation	MHRGAPINQSINLPPVNGSARRLPALPPYANHIHDETDDGPRYRDTGDRAGYDQSSRMVVANRNLPVDPDEEEQWMRSGGPSNRSDRRNPHLREPMLVARGAALALAGMSSEAYEGTYRPVGEGKSVRLPFSSRPVLRPAEDSRPVDRLIGQSLGLGRYADARIVGAARREIEEAYSLGEQEIDLAADSLAPLMQHVGMHDIRDINENSRSALLRPAENSSRQHDSRGGSQEDLLLVTTL

Target ORF information:

RefSeq Version	NM_001372868.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372868.1

ORF Insert Sequence:

ATGCATCGTG GAGCTCCAAT CAATCAATCA ATAAATCTTC CACCTGTCAA TGGATCAGCT 
CGACGTCTTC CCGCCCTTCC GCCGTACGCA AATCATATTC ACGATGAGAC GGACGACGGC 
CCCAGGTATC GGGATACTGG CGATCGAGCT GGTTACGATC AATCAAGCAG AATGGTTGTT 
GCCAATAGAA ATCTGCCTGT TGATCCCGAT GAAGAAGAGC AATGGATGAG AAGTGGTGGA 
CCGTCAAATC GATCTGACAG ACGAAACCCA CATCTACGAG AACCGATGCT TGTTGCTCGG 
GGAGCCGCTC TTGCACTCGC TGGAATGTCA TCAGAAGCCT ACGAAGGTAC TTATAGGCCG 
GTTGGAGAAG GAAAGTCTGT TCGACTTCCA TTCTCATCTC GACCTGTTCT CCGACCTGCC 
GAAGATTCAC GACCTGTTGA TCGACTGATC GGGCAATCAT TAGGACTTGG AAGGTACGCC 
GACGCTCGGA TTGTCGGAGC CGCTCGACGA GAAATTGAGG AAGCATATTC ACTTGGTGAA 
CAGGAAATTG ACTTGGCAGC AGACTCATTA GCTCCATTAA TGCAACATGT TGGAATGCAT 
GATATCAGAG ATATCAATGA AAACTCTCGA AGCGCACTTT TACGACCCGC CGAAAATTCA 
TCTCGGCAGC ATGATTCACG CGGAGGATCA CAAGAAGATC TACTTTTAGT TACAACTCTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-19 ( NM_171379.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_741442.1 Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), mRNA.

Price & Availability↑

CloneID	OCa343324
Clone ID Related Accession (Same CDS sequence)	NM_171379.4 , NM_171379.3
Accession Version	NM_171379.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Voltage-dependent L-type calcium channel subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_171379.3.

ATGTCAGTGT TAGCGAGTAT GATGTCATCG GGGGAGGACG AGGAGCAGGC GGCAGCTGAT 
GAGCAGGAGA GAACCGATCT GTGGCAGCAG ACATTACAGG CCGCCGTGGC AGCTTCATCA 
TCACAAGATG CCACAAAGAA ACGACCCGCA CAACGGAAAC CTCTTCGACA AACGAACGTC 
GTCGAAAGAT CCGAACGATC ACTTCTTTGC CTCTCCTTGA ATAATCCCAT CCGAAAATTG 
TGCATCAGTA TTGTTGAATG GAAACCATTC GAGTTTTTAA TATTGTTTAT GATATGTGCC 
AACTGTATCG CTCTAGCCAT CTATCAACCC TATCCTGCTC AAGATTCAGA CTATAAAAAT 
ACATTACTTG AAACAATTGA ATACGTGTTC ATTGTCGTAT TCACGATAGA ATGTGTGCTC 
AAAATAGTGG CTATGGGGTT TATGTTTCAT CCCAGTGCAT ATCTTCGAAA CGCATGGAAT 
ATTCTCGATT TTATTATTGT TGTCATAGGA CTTGTGAGTA CCATATTATC AAAAATGTCA 
ATTCAAGGAT TCGATGTAAA AGCTTTGAGA GCATTTCGTG TACTTCGACC GCTTCGTCTT 
GTATCAGGAG TACCAAGTTT ACAAGTTGTG CTCAACGCCA TCCTTCGAGC CATGATTCCT 
TTGCTCCACA TTGCCTTATT GGTTCTGTTT GTTATTTTGA TCTATGCTAT TATTGGATTA 
GAGTTATTCT GCGGAAAACT TCATTCAACA TGTATAGATC CAGCAACTGG ACAATTAGCG 
CAAAAAGATC CAACACCTTG TGGTACTGAT ACAGAAGGAA GTGCATTCAA ATGTCAACCA 
TCTGATTCTT TAACCAATAT GGGTGTTCGA TGGGAATGTT CAAGTAACAC AACATGGCCT 
GGTCCAAACA ATGGAATCAC GAACTTTGAT AACTTTGGAC TTGCTATGCT TACAGTATTC 
CAATGTGTAT CATTAGAAGG TTGGACAGAT GTAATGTACT GGGTAAATGA TGCTGTTGGC 
AGAGAGTGGC CGTGGATATA TTTTGTCACT CTTGTCATTC TTGGATCATT TTTCGTACTC 
AATCTTGTCT TGGGAGTCTT GTCTGGAGAA TTCTCAAAAG AACGAGAAAA GGCAAGAGCG 
AGAGGATTGT TTCAAAAATT TCGAGAAAAG CAACAACTTG AAGAAGATTT GAAAGGATAT 
CTCGACTGGA TCACACAAGC AGAAGATATT GAACCAGTGA ATGAAGATGA ACAAGAAGAT 
GAACCAGTTG CACAAACTGT AGTTGGAGAA GAAGCTGATG AAGAAGGAGA AGAACGAGTT 
GAAGATGTTA GACCGAGTAA ATGGGCAGCT AGAATGAAAC GATTAGAAAA ATTAAATAGG 
AGATGTCGAA GAGCTTGTAG AAGATTGGTC AAAAGTCAAA CATTTTATTG GCTGGTTATT 
CTTCTTGTGT TGCTCAATAC ATTAGTACTA ACATCTGAAC ATTATGGACA ATCCGAATGG 
CTCGATCATT TCCAAACAAT GGCAAACTTG TTCTTCGTAA TCCTTTTCTC GATGGAAATG 
CTCTTGAAAA TGTATTCGCT TGGGTTCACG ACTTACACGA CATCTCAATT TAATCGATTC 
GATTGTTTCG TCGTTATCAG TTCGATTTTG GAGTTTGTGC TTGTCTATTT CGATCTTATG 
AAGCCACTTG GAGTGTCTGT GCTGCGTTCA GCACGTCTTC TGAGAATATT CAAAGTTACA 
AAGTACTGGA CATCACTTCG TAATTTGGTA TCTTCACTTT TGAACTCTCT TCGATCCATC 
ATTTCCCTTC TGTTACTGCT TTTCCTGTTC ATTGTTATCT TTGCTTTATT GGGAATGCAA 
GTTTTCGGAG GAAAATTCAA TTTCAATCCA CAACAACCAA AACCAAGAGC CAATTTCGAT 
ACATTCGTTC AAGCATTGCT AACAGTATTC CAAATTCTTA CTGGTGAAGA TTGGAATACT 
GTAATGTATC ATGGAATTGA ATCATTTGGA GGTGTTGGAA CACTTGGAGT CATCGTCTGT 
ATCTACTATA TTGTTCTGTT CATTTGCGGT AATTACATTT TACTGAATGT CTTCTTGGCT 
ATTGCTGTCG ATAATTTGGC CGATGCAGAC AGTCTGACAA ATGCAGAAAA AGAAGAAGAG 
CAACAAGAAA TTGAAGGTGA AGATGAAGAA TTTGAGGAAG GAGAGGATGA AGGTGAAGAG 
CATGGAATGG ATGAACCAGA AGGAGATGAA GAAATGACAT CAGCAAGACC GAGAAGAATG 
AGTGAAGTTC CTGCTGCAAG TACGGTCAAA CCGATTCCAA AGGCTTCTTC GCTTTTCATT 
CTCAGTCATA CCAACTCTTT TCGAGTGTTT TGCAACATGG TTGTCAATCA TTCATATTTC 
ACCAACGCCG TTCTTTTTTG TATTTTGGTA TCATCTGCTA TGTTAGCTGC TGAAGATCCT 
CTACAAGCAA ACTCAACTAG AAATATGATT CTGAATTACT TTGACTACTT CTTCACGTCT 
GTGTTCACAG TTGAGATCAC GTTGAAAGTC ATCGTATTTG GTTTGGTATT CCACAAAGGA 
TCATTCTGTC GAAATGCATT CAATCTTCTC GATATCCTCG TCGTTGCAGT ATCATTAACA 
TCTTTTGTAC TTCGAACTGA TGCAATGTCT GTCGTCAAGA TTCTTCGTGT GCTCCGTGTG 
CTCCGTCCAC TTCGTGCAAT TAACCGAGCC AAGGGTTTGA AACATGTTGT ACAATGTGTA 
ATTGTTGCTG TAAAGACTAT CGGAAATATC ATGTTGGTCA CATTCATGCT TCAATTCATG 
TTTGCAATTA TCGGAGTTCA ACTTTTCAAG GGAACATTCT TCCTTTGCAA TGATCTTTCG 
AAAATGACAG AAGCAGAATG CCGTGGAGAA TATATTCATT ATGAAGATGG AGATCCAACA 
AAACCAGTTT CAAAGAAAAG AGTTTGGTCG AATAATGACT TCAATTTTGA TAACGTCGGA 
GATGCGATGA TTTCACTTTT CGTAGTTTCA ACTTTCGAAG GATGGCCACA ACTTCTTTAT 
GTTGCAATTG ATTCAAATGA AGAAGATAAG GGACCTATAC ATAATTCAAG ACAAGCTGTT 
GCTTTGTTCT TCATTGCTTT CATCATTGTC ATCGCATTCT TCATGATGAA CATCTTCGTA 
GGTTTCGTTA TCGTTACATT CCAAAATGAA GGAGAACGTG AATACGAGAA TTGTGAATTG 
GACAAGAATC AAAGAAAATG TATTGAATTT GCATTGAAAG CTAAACCACA CAGAAGATAC 
ATTCCACGAA ACAGATTACA ATACAGAGTT TGGTGGTTTG TCACATCAAG AGCTTTTGAG 
TATGTCATCT TTCTGATTAT CGTCATGAAT ACAGTATCTT TGGCGTGCAA GCATTATCCA 
AGTTCGAGAG GATTCGAAGA CTTTTTGGAT GTGTTCAATT TGATTTTCAC TGGAGTTTTC 
GCATTTGAAG CTGTTCTGAA GATTGTTGCA TTGAATCCGA AAAATTATAT TTCCGATCGA 
TGGAATGTCT TTGATTTGTT GGTTGTTGTC GGATCATTTA TTGATATCAC ATATGGAAAA 
TTGAATCCAG GAGGAACAAA TCTTATTTCA ATCAACTTTT TCCGTCTGTT CCGTGTGATG 
CGTCTCGTGA AGCTGCTTTC AAGAGGAGAA GGAATTCGAA CACTTTTATG GACATTCATG 
AAATCATTTC AAGCTCTTCC ATACGTCGCC TTGCTCATCG TTTTGCTCTT TTTCATTTAT 
GCTGTCATCG GAATGCAATT CTTTGGAAAA GTTGCACTGG ACGATTCAAC ATCTATACAC 
AGGAACAATA ACTTCCATTC GTTCCCTGCA GCGATTTTGG TGTTATTCAG ATCAGCAACT 
GGAGAAGCCT GGCAAGATAT CATGCTTTCG TGTTCGGATC GAGAAGATGT CAGATGTGAT 
CCAATGTCTG ATGATTACCA TAAAGGAGGC CTCAACGAAT CTAGATGTGG AAACAATTTT 
GCATATCCAT ACTTCATTTC ATTCTTCATG CTTTGCTCTT TCCTTGTCAT CAATCTTTTC 
GTAGCTGTTA TCATGGACAA TTTCGATTAT TTAACAAGAG ATTGGTCAAT TCTTGGGCCA 
CATCATTTGG AAGAATTTGT AAGATTATGG TCTGAATATG ATCCAGATGC AAAAGGACGA 
ATTAAACATC TTGACGTCGT CACACTTCTT CGAAAAATCT CTCCTCCACT TGGATTTGGA 
AAACTGTGTC CTCATCGATT GGCCTGTAAG AGATTGGTAT CAATGAATAT GCCCTTGAAC 
TCGGATGGTA CTGTATGCTT CAATGCAACT CTTTTTGCTC TCGTTCGTAC CAATTTGAAG 
ATTTATACTG AAGGAAACAT CGACGAAGCA AATGAACAGC TGCGATCAGC AATTAAACGA 
ATTTGGAAAC GAACTCATAA GGATTTGTTG GATGAGGTTG TTCCACCGGC AGGAAAAGAA 
GATGATGTGA CCGTTGGAAA ATTCTATGCC ACCTTCCTCA TTCAAGACTA TTTCCGTCGT 
TTCAAGAAGC GAAAAGAGAT GGAAGCAAAG GGTGTCTTAC CAGCTCAAAC ACCTCAAGCG 
ATGGCTCTTC AGGCCGGACT CCGCACGCTA CACGAGATCG GACCAGAATT GAAAAGAGCG 
ATTTCTGGAA ACTTGGAGAC CGACTTCAAC TTTGACGAAC CAGAGCCTCA ACACAGAAGA 
CCACACTCTC TTTTCAACAA TTTGGTACAT CGTCTATCTG GTGCCGGATC CAAATCTCCT 
ACTGAACACG AACGTATAGA AAAGGGCTCT ACACTTTTGC CATTCCAACC ACGCTCTTTC 
TCTCCAACTC ATTCACTTGC TGGCGCTGAA GGATCACCAG TACCATCACA AATGCATCGT 
GGAGCTCCAA TCAATCAATC AATAAATCTT CCACCTGTCA ATGGATCAGC TCGACGTCTT 
CCCGCCCTTC CGCCGTACGC AAATCATATT CACGATGAGA CGGACGACGG CCCCAGGTAT 
CGGGATACTG GCGATCGAGC TGGTTACGAT CAATCAAGCA GAATGGTTGT TGCCAATAGA 
AAGTACGCCG ACGCTCGGAT TGTCGGAGCC GCTCGACGAG AAATTGAGGA AGCATATTCA 
CTTGGTGAAC AGGAAATTGA CTTGGCAGCA GACTCATTAG CTCCATTAAT GCAACATGTT 
GGAATGCATG ATATCAGAGA TATCAATGAA AACTCTCGAA GCGCACTTTT ACGACCCGCC 
GAAAATTCAT CTCGGCAGCA TGATTCACGC GGAGGATCAC AAGAAGATCT ACTTTTAGTT 
ACAACTCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_741442.1
CDS	123..5474
Translation	MSVLASMMSSGEDEEQAAADEQERTDLWQQTLQAAVAASSSQDATKKRPAQRKPLRQTNVVERSERSLLCLSLNNPIRKLCISIVEWKPFEFLILFMICANCIALAIYQPYPAQDSDYKNTLLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNILDFIIVVIGLVSTILSKMSIQGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILRAMIPLLHIALLVLFVILIYAIIGLELFCGKLHSTCIDPATGQLAQKDPTPCGTDTEGSAFKCQPSDSLTNMGVRWECSSNTTWPGPNNGITNFDNFGLAMLTVFQCVSLEGWTDVMYWVNDAVGREWPWIYFVTLVILGSFFVLNLVLGVLSGEFSKEREKARARGLFQKFREKQQLEEDLKGYLDWITQAEDIEPVNEDEQEDEPVAQTVVGEEADEEGEERVEDVRPSKWAARMKRLEKLNRRCRRACRRLVKSQTFYWLVILLVLLNTLVLTSEHYGQSEWLDHFQTMANLFFVILFSMEMLLKMYSLGFTTYTTSQFNRFDCFVVISSILEFVLVYFDLMKPLGVSVLRSARLLRIFKVTKYWTSLRNLVSSLLNSLRSIISLLLLLFLFIVIFALLGMQVFGGKFNFNPQQPKPRANFDTFVQALLTVFQILTGEDWNTVMYHGIESFGGVGTLGVIVCIYYIVLFICGNYILLNVFLAIAVDNLADADSLTNAEKEEEQQEIEGEDEEFEEGEDEGEEHGMDEPEGDEEMTSARPRRMSEVPAASTVKPIPKASSLFILSHTNSFRVFCNMVVNHSYFTNAVLFCILVSSAMLAAEDPLQANSTRNMILNYFDYFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRTDAMSVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIMLVTFMLQFMFAIIGVQLFKGTFFLCNDLSKMTEAECRGEYIHYEDGDPTKPVSKKRVWSNNDFNFDNVGDAMISLFVVSTFEGWPQLLYVAIDSNEEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIFVGFVIVTFQNEGEREYENCELDKNQRKCIEFALKAKPHRRYIPRNRLQYRVWWFVTSRAFEYVIFLIIVMNTVSLACKHYPSSRGFEDFLDVFNLIFTGVFAFEAVLKIVALNPKNYISDRWNVFDLLVVVGSFIDITYGKLNPGGTNLISINFFRLFRVMRLVKLLSRGEGIRTLLWTFMKSFQALPYVALLIVLLFFIYAVIGMQFFGKVALDDSTSIHRNNNFHSFPAAILVLFRSATGEAWQDIMLSCSDREDVRCDPMSDDYHKGGLNESRCGNNFAYPYFISFFMLCSFLVINLFVAVIMDNFDYLTRDWSILGPHHLEEFVRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRLACKRLVSMNMPLNSDGTVCFNATLFALVRTNLKIYTEGNIDEANEQLRSAIKRIWKRTHKDLLDEVVPPAGKEDDVTVGKFYATFLIQDYFRRFKKRKEMEAKGVLPAQTPQAMALQAGLRTLHEIGPELKRAISGNLETDFNFDEPEPQHRRPHSLFNNLVHRLSGAGSKSPTEHERIEKGSTLLPFQPRSFSPTHSLAGAEGSPVPSQMHRGAPINQSINLPPVNGSARRLPALPPYANHIHDETDDGPRYRDTGDRAGYDQSSRMVVANRKYADARIVGAARREIEEAYSLGEQEIDLAADSLAPLMQHVGMHDIRDINENSRSALLRPAENSSRQHDSRGGSQEDLLLVTTL

Target ORF information:

RefSeq Version	NM_171379.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_171379.4

ORF Insert Sequence:

ATGTCAGTGT TAGCGAGTAT GATGTCATCG GGGGAGGACG AGGAGCAGGC GGCAGCTGAT 
GAGCAGGAGA GAACCGATCT GTGGCAGCAG ACATTACAGG CCGCCGTGGC AGCTTCATCA 
TCACAAGATG CCACAAAGAA ACGACCCGCA CAACGGAAAC CTCTTCGACA AACGAACGTC 
GTCGAAAGAT CCGAACGATC ACTTCTTTGC CTCTCCTTGA ATAATCCCAT CCGAAAATTG 
TGCATCAGTA TTGTTGAATG GAAACCATTC GAGTTTTTAA TATTGTTTAT GATATGTGCC 
AACTGTATCG CTCTAGCCAT CTATCAACCC TATCCTGCTC AAGATTCAGA CTATAAAAAT 
ACATTACTTG AAACAATTGA ATACGTGTTC ATTGTCGTAT TCACGATAGA ATGTGTGCTC 
AAAATAGTGG CTATGGGGTT TATGTTTCAT CCCAGTGCAT ATCTTCGAAA CGCATGGAAT 
ATTCTCGATT TTATTATTGT TGTCATAGGA CTTGTGAGTA CCATATTATC AAAAATGTCA 
ATTCAAGGAT TCGATGTAAA AGCTTTGAGA GCATTTCGTG TACTTCGACC GCTTCGTCTT 
GTATCAGGAG TACCAAGTTT ACAAGTTGTG CTCAACGCCA TCCTTCGAGC CATGATTCCT 
TTGCTCCACA TTGCCTTATT GGTTCTGTTT GTTATTTTGA TCTATGCTAT TATTGGATTA 
GAGTTATTCT GCGGAAAACT TCATTCAACA TGTATAGATC CAGCAACTGG ACAATTAGCG 
CAAAAAGATC CAACACCTTG TGGTACTGAT ACAGAAGGAA GTGCATTCAA ATGTCAACCA 
TCTGATTCTT TAACCAATAT GGGTGTTCGA TGGGAATGTT CAAGTAACAC AACATGGCCT 
GGTCCAAACA ATGGAATCAC GAACTTTGAT AACTTTGGAC TTGCTATGCT TACAGTATTC 
CAATGTGTAT CATTAGAAGG TTGGACAGAT GTAATGTACT GGGTAAATGA TGCTGTTGGC 
AGAGAGTGGC CGTGGATATA TTTTGTCACT CTTGTCATTC TTGGATCATT TTTCGTACTC 
AATCTTGTCT TGGGAGTCTT GTCTGGAGAA TTCTCAAAAG AACGAGAAAA GGCAAGAGCG 
AGAGGATTGT TTCAAAAATT TCGAGAAAAG CAACAACTTG AAGAAGATTT GAAAGGATAT 
CTCGACTGGA TCACACAAGC AGAAGATATT GAACCAGTGA ATGAAGATGA ACAAGAAGAT 
GAACCAGTTG CACAAACTGT AGTTGGAGAA GAAGCTGATG AAGAAGGAGA AGAACGAGTT 
GAAGATGTTA GACCGAGTAA ATGGGCAGCT AGAATGAAAC GATTAGAAAA ATTAAATAGG 
AGATGTCGAA GAGCTTGTAG AAGATTGGTC AAAAGTCAAA CATTTTATTG GCTGGTTATT 
CTTCTTGTGT TGCTCAATAC ATTAGTACTA ACATCTGAAC ATTATGGACA ATCCGAATGG 
CTCGATCATT TCCAAACAAT GGCAAACTTG TTCTTCGTAA TCCTTTTCTC GATGGAAATG 
CTCTTGAAAA TGTATTCGCT TGGGTTCACG ACTTACACGA CATCTCAATT TAATCGATTC 
GATTGTTTCG TCGTTATCAG TTCGATTTTG GAGTTTGTGC TTGTCTATTT CGATCTTATG 
AAGCCACTTG GAGTGTCTGT GCTGCGTTCA GCACGTCTTC TGAGAATATT CAAAGTTACA 
AAGTACTGGA CATCACTTCG TAATTTGGTA TCTTCACTTT TGAACTCTCT TCGATCCATC 
ATTTCCCTTC TGTTACTGCT TTTCCTGTTC ATTGTTATCT TTGCTTTATT GGGAATGCAA 
GTTTTCGGAG GAAAATTCAA TTTCAATCCA CAACAACCAA AACCAAGAGC CAATTTCGAT 
ACATTCGTTC AAGCATTGCT AACAGTATTC CAAATTCTTA CTGGTGAAGA TTGGAATACT 
GTAATGTATC ATGGAATTGA ATCATTTGGA GGTGTTGGAA CACTTGGAGT CATCGTCTGT 
ATCTACTATA TTGTTCTGTT CATTTGCGGT AATTACATTT TACTGAATGT CTTCTTGGCT 
ATTGCTGTCG ATAATTTGGC CGATGCAGAC AGTCTGACAA ATGCAGAAAA AGAAGAAGAG 
CAACAAGAAA TTGAAGGTGA AGATGAAGAA TTTGAGGAAG GAGAGGATGA AGGTGAAGAG 
CATGGAATGG ATGAACCAGA AGGAGATGAA GAAATGACAT CAGCAAGACC GAGAAGAATG 
AGTGAAGTTC CTGCTGCAAG TACGGTCAAA CCGATTCCAA AGGCTTCTTC GCTTTTCATT 
CTCAGTCATA CCAACTCTTT TCGAGTGTTT TGCAACATGG TTGTCAATCA TTCATATTTC 
ACCAACGCCG TTCTTTTTTG TATTTTGGTA TCATCTGCTA TGTTAGCTGC TGAAGATCCT 
CTACAAGCAA ACTCAACTAG AAATATGATT CTGAATTACT TTGACTACTT CTTCACGTCT 
GTGTTCACAG TTGAGATCAC GTTGAAAGTC ATCGTATTTG GTTTGGTATT CCACAAAGGA 
TCATTCTGTC GAAATGCATT CAATCTTCTC GATATCCTCG TCGTTGCAGT ATCATTAACA 
TCTTTTGTAC TTCGAACTGA TGCAATGTCT GTCGTCAAGA TTCTTCGTGT GCTCCGTGTG 
CTCCGTCCAC TTCGTGCAAT TAACCGAGCC AAGGGTTTGA AACATGTTGT ACAATGTGTA 
ATTGTTGCTG TAAAGACTAT CGGAAATATC ATGTTGGTCA CATTCATGCT TCAATTCATG 
TTTGCAATTA TCGGAGTTCA ACTTTTCAAG GGAACATTCT TCCTTTGCAA TGATCTTTCG 
AAAATGACAG AAGCAGAATG CCGTGGAGAA TATATTCATT ATGAAGATGG AGATCCAACA 
AAACCAGTTT CAAAGAAAAG AGTTTGGTCG AATAATGACT TCAATTTTGA TAACGTCGGA 
GATGCGATGA TTTCACTTTT CGTAGTTTCA ACTTTCGAAG GATGGCCACA ACTTCTTTAT 
GTTGCAATTG ATTCAAATGA AGAAGATAAG GGACCTATAC ATAATTCAAG ACAAGCTGTT 
GCTTTGTTCT TCATTGCTTT CATCATTGTC ATCGCATTCT TCATGATGAA CATCTTCGTA 
GGTTTCGTTA TCGTTACATT CCAAAATGAA GGAGAACGTG AATACGAGAA TTGTGAATTG 
GACAAGAATC AAAGAAAATG TATTGAATTT GCATTGAAAG CTAAACCACA CAGAAGATAC 
ATTCCACGAA ACAGATTACA ATACAGAGTT TGGTGGTTTG TCACATCAAG AGCTTTTGAG 
TATGTCATCT TTCTGATTAT CGTCATGAAT ACAGTATCTT TGGCGTGCAA GCATTATCCA 
AGTTCGAGAG GATTCGAAGA CTTTTTGGAT GTGTTCAATT TGATTTTCAC TGGAGTTTTC 
GCATTTGAAG CTGTTCTGAA GATTGTTGCA TTGAATCCGA AAAATTATAT TTCCGATCGA 
TGGAATGTCT TTGATTTGTT GGTTGTTGTC GGATCATTTA TTGATATCAC ATATGGAAAA 
TTGAATCCAG GAGGAACAAA TCTTATTTCA ATCAACTTTT TCCGTCTGTT CCGTGTGATG 
CGTCTCGTGA AGCTGCTTTC AAGAGGAGAA GGAATTCGAA CACTTTTATG GACATTCATG 
AAATCATTTC AAGCTCTTCC ATACGTCGCC TTGCTCATCG TTTTGCTCTT TTTCATTTAT 
GCTGTCATCG GAATGCAATT CTTTGGAAAA GTTGCACTGG ACGATTCAAC ATCTATACAC 
AGGAACAATA ACTTCCATTC GTTCCCTGCA GCGATTTTGG TGTTATTCAG ATCAGCAACT 
GGAGAAGCCT GGCAAGATAT CATGCTTTCG TGTTCGGATC GAGAAGATGT CAGATGTGAT 
CCAATGTCTG ATGATTACCA TAAAGGAGGC CTCAACGAAT CTAGATGTGG AAACAATTTT 
GCATATCCAT ACTTCATTTC ATTCTTCATG CTTTGCTCTT TCCTTGTCAT CAATCTTTTC 
GTAGCTGTTA TCATGGACAA TTTCGATTAT TTAACAAGAG ATTGGTCAAT TCTTGGGCCA 
CATCATTTGG AAGAATTTGT AAGATTATGG TCTGAATATG ATCCAGATGC AAAAGGACGA 
ATTAAACATC TTGACGTCGT CACACTTCTT CGAAAAATCT CTCCTCCACT TGGATTTGGA 
AAACTGTGTC CTCATCGATT GGCCTGTAAG AGATTGGTAT CAATGAATAT GCCCTTGAAC 
TCGGATGGTA CTGTATGCTT CAATGCAACT CTTTTTGCTC TCGTTCGTAC CAATTTGAAG 
ATTTATACTG AAGGAAACAT CGACGAAGCA AATGAACAGC TGCGATCAGC AATTAAACGA 
ATTTGGAAAC GAACTCATAA GGATTTGTTG GATGAGGTTG TTCCACCGGC AGGAAAAGAA 
GATGATGTGA CCGTTGGAAA ATTCTATGCC ACCTTCCTCA TTCAAGACTA TTTCCGTCGT 
TTCAAGAAGC GAAAAGAGAT GGAAGCAAAG GGTGTCTTAC CAGCTCAAAC ACCTCAAGCG 
ATGGCTCTTC AGGCCGGACT CCGCACGCTA CACGAGATCG GACCAGAATT GAAAAGAGCG 
ATTTCTGGAA ACTTGGAGAC CGACTTCAAC TTTGACGAAC CAGAGCCTCA ACACAGAAGA 
CCACACTCTC TTTTCAACAA TTTGGTACAT CGTCTATCTG GTGCCGGATC CAAATCTCCT 
ACTGAACACG AACGTATAGA AAAGGGCTCT ACACTTTTGC CATTCCAACC ACGCTCTTTC 
TCTCCAACTC ATTCACTTGC TGGCGCTGAA GGATCACCAG TACCATCACA AATGCATCGT 
GGAGCTCCAA TCAATCAATC AATAAATCTT CCACCTGTCA ATGGATCAGC TCGACGTCTT 
CCCGCCCTTC CGCCGTACGC AAATCATATT CACGATGAGA CGGACGACGG CCCCAGGTAT 
CGGGATACTG GCGATCGAGC TGGTTACGAT CAATCAAGCA GAATGGTTGT TGCCAATAGA 
AAGTACGCCG ACGCTCGGAT TGTCGGAGCC GCTCGACGAG AAATTGAGGA AGCATATTCA 
CTTGGTGAAC AGGAAATTGA CTTGGCAGCA GACTCATTAG CTCCATTAAT GCAACATGTT 
GGAATGCATG ATATCAGAGA TATCAATGAA AACTCTCGAA GCGCACTTTT ACGACCCGCC 
GAAAATTCAT CTCGGCAGCA TGATTCACGC GGAGGATCAC AAGAAGATCT ACTTTTAGTT 
ACAACTCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-19 ( NM_171379.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_741442.1 Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), partial mRNA.

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CloneID	OCa343324
Clone ID Related Accession (Same CDS sequence)	NM_171379.4 , NM_171379.3
Accession Version	NM_171379.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Voltage-dependent L-type calcium channel subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_171379.2. COMPLETENESS: incomplete on both ends.

ATGTCAGTGT TAGCGAGTAT GATGTCATCG GGGGAGGACG AGGAGCAGGC GGCAGCTGAT 
GAGCAGGAGA GAACCGATCT GTGGCAGCAG ACATTACAGG CCGCCGTGGC AGCTTCATCA 
TCACAAGATG CCACAAAGAA ACGACCCGCA CAACGGAAAC CTCTTCGACA AACGAACGTC 
GTCGAAAGAT CCGAACGATC ACTTCTTTGC CTCTCCTTGA ATAATCCCAT CCGAAAATTG 
TGCATCAGTA TTGTTGAATG GAAACCATTC GAGTTTTTAA TATTGTTTAT GATATGTGCC 
AACTGTATCG CTCTAGCCAT CTATCAACCC TATCCTGCTC AAGATTCAGA CTATAAAAAT 
ACATTACTTG AAACAATTGA ATACGTGTTC ATTGTCGTAT TCACGATAGA ATGTGTGCTC 
AAAATAGTGG CTATGGGGTT TATGTTTCAT CCCAGTGCAT ATCTTCGAAA CGCATGGAAT 
ATTCTCGATT TTATTATTGT TGTCATAGGA CTTGTGAGTA CCATATTATC AAAAATGTCA 
ATTCAAGGAT TCGATGTAAA AGCTTTGAGA GCATTTCGTG TACTTCGACC GCTTCGTCTT 
GTATCAGGAG TACCAAGTTT ACAAGTTGTG CTCAACGCCA TCCTTCGAGC CATGATTCCT 
TTGCTCCACA TTGCCTTATT GGTTCTGTTT GTTATTTTGA TCTATGCTAT TATTGGATTA 
GAGTTATTCT GCGGAAAACT TCATTCAACA TGTATAGATC CAGCAACTGG ACAATTAGCG 
CAAAAAGATC CAACACCTTG TGGTACTGAT ACAGAAGGAA GTGCATTCAA ATGTCAACCA 
TCTGATTCTT TAACCAATAT GGGTGTTCGA TGGGAATGTT CAAGTAACAC AACATGGCCT 
GGTCCAAACA ATGGAATCAC GAACTTTGAT AACTTTGGAC TTGCTATGCT TACAGTATTC 
CAATGTGTAT CATTAGAAGG TTGGACAGAT GTAATGTACT GGGTAAATGA TGCTGTTGGC 
AGAGAGTGGC CGTGGATATA TTTTGTCACT CTTGTCATTC TTGGATCATT TTTCGTACTC 
AATCTTGTCT TGGGAGTCTT GTCTGGAGAA TTCTCAAAAG AACGAGAAAA GGCAAGAGCG 
AGAGGATTGT TTCAAAAATT TCGAGAAAAG CAACAACTTG AAGAAGATTT GAAAGGATAT 
CTCGACTGGA TCACACAAGC AGAAGATATT GAACCAGTGA ATGAAGATGA ACAAGAAGAT 
GAACCAGTTG CACAAACTGT AGTTGGAGAA GAAGCTGATG AAGAAGGAGA AGAACGAGTT 
GAAGATGTTA GACCGAGTAA ATGGGCAGCT AGAATGAAAC GATTAGAAAA ATTAAATAGG 
AGATGTCGAA GAGCTTGTAG AAGATTGGTC AAAAGTCAAA CATTTTATTG GCTGGTTATT 
CTTCTTGTGT TGCTCAATAC ATTAGTACTA ACATCTGAAC ATTATGGACA ATCCGAATGG 
CTCGATCATT TCCAAACAAT GGCAAACTTG TTCTTCGTAA TCCTTTTCTC GATGGAAATG 
CTCTTGAAAA TGTATTCGCT TGGGTTCACG ACTTACACGA CATCTCAATT TAATCGATTC 
GATTGTTTCG TCGTTATCAG TTCGATTTTG GAGTTTGTGC TTGTCTATTT CGATCTTATG 
AAGCCACTTG GAGTGTCTGT GCTGCGTTCA GCACGTCTTC TGAGAATATT CAAAGTTACA 
AAGTACTGGA CATCACTTCG TAATTTGGTA TCTTCACTTT TGAACTCTCT TCGATCCATC 
ATTTCCCTTC TGTTACTGCT TTTCCTGTTC ATTGTTATCT TTGCTTTATT GGGAATGCAA 
GTTTTCGGAG GAAAATTCAA TTTCAATCCA CAACAACCAA AACCAAGAGC CAATTTCGAT 
ACATTCGTTC AAGCATTGCT AACAGTATTC CAAATTCTTA CTGGTGAAGA TTGGAATACT 
GTAATGTATC ATGGAATTGA ATCATTTGGA GGTGTTGGAA CACTTGGAGT CATCGTCTGT 
ATCTACTATA TTGTTCTGTT CATTTGCGGT AATTACATTT TACTGAATGT CTTCTTGGCT 
ATTGCTGTCG ATAATTTGGC CGATGCAGAC AGTCTGACAA ATGCAGAAAA AGAAGAAGAG 
CAACAAGAAA TTGAAGGTGA AGATGAAGAA TTTGAGGAAG GAGAGGATGA AGGTGAAGAG 
CATGGAATGG ATGAACCAGA AGGAGATGAA GAAATGACAT CAGCAAGACC GAGAAGAATG 
AGTGAAGTTC CTGCTGCAAG TACGGTCAAA CCGATTCCAA AGGCTTCTTC GCTTTTCATT 
CTCAGTCATA CCAACTCTTT TCGAGTGTTT TGCAACATGG TTGTCAATCA TTCATATTTC 
ACCAACGCCG TTCTTTTTTG TATTTTGGTA TCATCTGCTA TGTTAGCTGC TGAAGATCCT 
CTACAAGCAA ACTCAACTAG AAATATGATT CTGAATTACT TTGACTACTT CTTCACGTCT 
GTGTTCACAG TTGAGATCAC GTTGAAAGTC ATCGTATTTG GTTTGGTATT CCACAAAGGA 
TCATTCTGTC GAAATGCATT CAATCTTCTC GATATCCTCG TCGTTGCAGT ATCATTAACA 
TCTTTTGTAC TTCGAACTGA TGCAATGTCT GTCGTCAAGA TTCTTCGTGT GCTCCGTGTG 
CTCCGTCCAC TTCGTGCAAT TAACCGAGCC AAGGGTTTGA AACATGTTGT ACAATGTGTA 
ATTGTTGCTG TAAAGACTAT CGGAAATATC ATGTTGGTCA CATTCATGCT TCAATTCATG 
TTTGCAATTA TCGGAGTTCA ACTTTTCAAG GGAACATTCT TCCTTTGCAA TGATCTTTCG 
AAAATGACAG AAGCAGAATG CCGTGGAGAA TATATTCATT ATGAAGATGG AGATCCAACA 
AAACCAGTTT CAAAGAAAAG AGTTTGGTCG AATAATGACT TCAATTTTGA TAACGTCGGA 
GATGCGATGA TTTCACTTTT CGTAGTTTCA ACTTTCGAAG GATGGCCACA ACTTCTTTAT 
GTTGCAATTG ATTCAAATGA AGAAGATAAG GGACCTATAC ATAATTCAAG ACAAGCTGTT 
GCTTTGTTCT TCATTGCTTT CATCATTGTC ATCGCATTCT TCATGATGAA CATCTTCGTA 
GGTTTCGTTA TCGTTACATT CCAAAATGAA GGAGAACGTG AATACGAGAA TTGTGAATTG 
GACAAGAATC AAAGAAAATG TATTGAATTT GCATTGAAAG CTAAACCACA CAGAAGATAC 
ATTCCACGAA ACAGATTACA ATACAGAGTT TGGTGGTTTG TCACATCAAG AGCTTTTGAG 
TATGTCATCT TTCTGATTAT CGTCATGAAT ACAGTATCTT TGGCGTGCAA GCATTATCCA 
AGTTCGAGAG GATTCGAAGA CTTTTTGGAT GTGTTCAATT TGATTTTCAC TGGAGTTTTC 
GCATTTGAAG CTGTTCTGAA GATTGTTGCA TTGAATCCGA AAAATTATAT TTCCGATCGA 
TGGAATGTCT TTGATTTGTT GGTTGTTGTC GGATCATTTA TTGATATCAC ATATGGAAAA 
TTGAATCCAG GAGGAACAAA TCTTATTTCA ATCAACTTTT TCCGTCTGTT CCGTGTGATG 
CGTCTCGTGA AGCTGCTTTC AAGAGGAGAA GGAATTCGAA CACTTTTATG GACATTCATG 
AAATCATTTC AAGCTCTTCC ATACGTCGCC TTGCTCATCG TTTTGCTCTT TTTCATTTAT 
GCTGTCATCG GAATGCAATT CTTTGGAAAA GTTGCACTGG ACGATTCAAC ATCTATACAC 
AGGAACAATA ACTTCCATTC GTTCCCTGCA GCGATTTTGG TGTTATTCAG ATCAGCAACT 
GGAGAAGCCT GGCAAGATAT CATGCTTTCG TGTTCGGATC GAGAAGATGT CAGATGTGAT 
CCAATGTCTG ATGATTACCA TAAAGGAGGC CTCAACGAAT CTAGATGTGG AAACAATTTT 
GCATATCCAT ACTTCATTTC ATTCTTCATG CTTTGCTCTT TCCTTGTCAT CAATCTTTTC 
GTAGCTGTTA TCATGGACAA TTTCGATTAT TTAACAAGAG ATTGGTCAAT TCTTGGGCCA 
CATCATTTGG AAGAATTTGT AAGATTATGG TCTGAATATG ATCCAGATGC AAAAGGACGA 
ATTAAACATC TTGACGTCGT CACACTTCTT CGAAAAATCT CTCCTCCACT TGGATTTGGA 
AAACTGTGTC CTCATCGATT GGCCTGTAAG AGATTGGTAT CAATGAATAT GCCCTTGAAC 
TCGGATGGTA CTGTATGCTT CAATGCAACT CTTTTTGCTC TCGTTCGTAC CAATTTGAAG 
ATTTATACTG AAGGAAACAT CGACGAAGCA AATGAACAGC TGCGATCAGC AATTAAACGA 
ATTTGGAAAC GAACTCATAA GGATTTGTTG GATGAGGTTG TTCCACCGGC AGGAAAAGAA 
GATGATGTGA CCGTTGGAAA ATTCTATGCC ACCTTCCTCA TTCAAGACTA TTTCCGTCGT 
TTCAAGAAGC GAAAAGAGAT GGAAGCAAAG GGTGTCTTAC CAGCTCAAAC ACCTCAAGCG 
ATGGCTCTTC AGGCCGGACT CCGCACGCTA CACGAGATCG GACCAGAATT GAAAAGAGCG 
ATTTCTGGAA ACTTGGAGAC CGACTTCAAC TTTGACGAAC CAGAGCCTCA ACACAGAAGA 
CCACACTCTC TTTTCAACAA TTTGGTACAT CGTCTATCTG GTGCCGGATC CAAATCTCCT 
ACTGAACACG AACGTATAGA AAAGGGCTCT ACACTTTTGC CATTCCAACC ACGCTCTTTC 
TCTCCAACTC ATTCACTTGC TGGCGCTGAA GGATCACCAG TACCATCACA AATGCATCGT 
GGAGCTCCAA TCAATCAATC AATAAATCTT CCACCTGTCA ATGGATCAGC TCGACGTCTT 
CCCGCCCTTC CGCCGTACGC AAATCATATT CACGATGAGA CGGACGACGG CCCCAGGTAT 
CGGGATACTG GCGATCGAGC TGGTTACGAT CAATCAAGCA GAATGGTTGT TGCCAATAGA 
AAGTACGCCG ACGCTCGGAT TGTCGGAGCC GCTCGACGAG AAATTGAGGA AGCATATTCA 
CTTGGTGAAC AGGAAATTGA CTTGGCAGCA GACTCATTAG CTCCATTAAT GCAACATGTT 
GGAATGCATG ATATCAGAGA TATCAATGAA AACTCTCGAA GCGCACTTTT ACGACCCGCC 
GAAAATTCAT CTCGGCAGCA TGATTCACGC GGAGGATCAC AAGAAGATCT ACTTTTAGTT 
ACAACTCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_741442.1
CDS	1..5352
Translation	MSVLASMMSSGEDEEQAAADEQERTDLWQQTLQAAVAASSSQDATKKRPAQRKPLRQTNVVERSERSLLCLSLNNPIRKLCISIVEWKPFEFLILFMICANCIALAIYQPYPAQDSDYKNTLLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNILDFIIVVIGLVSTILSKMSIQGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILRAMIPLLHIALLVLFVILIYAIIGLELFCGKLHSTCIDPATGQLAQKDPTPCGTDTEGSAFKCQPSDSLTNMGVRWECSSNTTWPGPNNGITNFDNFGLAMLTVFQCVSLEGWTDVMYWVNDAVGREWPWIYFVTLVILGSFFVLNLVLGVLSGEFSKEREKARARGLFQKFREKQQLEEDLKGYLDWITQAEDIEPVNEDEQEDEPVAQTVVGEEADEEGEERVEDVRPSKWAARMKRLEKLNRRCRRACRRLVKSQTFYWLVILLVLLNTLVLTSEHYGQSEWLDHFQTMANLFFVILFSMEMLLKMYSLGFTTYTTSQFNRFDCFVVISSILEFVLVYFDLMKPLGVSVLRSARLLRIFKVTKYWTSLRNLVSSLLNSLRSIISLLLLLFLFIVIFALLGMQVFGGKFNFNPQQPKPRANFDTFVQALLTVFQILTGEDWNTVMYHGIESFGGVGTLGVIVCIYYIVLFICGNYILLNVFLAIAVDNLADADSLTNAEKEEEQQEIEGEDEEFEEGEDEGEEHGMDEPEGDEEMTSARPRRMSEVPAASTVKPIPKASSLFILSHTNSFRVFCNMVVNHSYFTNAVLFCILVSSAMLAAEDPLQANSTRNMILNYFDYFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRTDAMSVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIMLVTFMLQFMFAIIGVQLFKGTFFLCNDLSKMTEAECRGEYIHYEDGDPTKPVSKKRVWSNNDFNFDNVGDAMISLFVVSTFEGWPQLLYVAIDSNEEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIFVGFVIVTFQNEGEREYENCELDKNQRKCIEFALKAKPHRRYIPRNRLQYRVWWFVTSRAFEYVIFLIIVMNTVSLACKHYPSSRGFEDFLDVFNLIFTGVFAFEAVLKIVALNPKNYISDRWNVFDLLVVVGSFIDITYGKLNPGGTNLISINFFRLFRVMRLVKLLSRGEGIRTLLWTFMKSFQALPYVALLIVLLFFIYAVIGMQFFGKVALDDSTSIHRNNNFHSFPAAILVLFRSATGEAWQDIMLSCSDREDVRCDPMSDDYHKGGLNESRCGNNFAYPYFISFFMLCSFLVINLFVAVIMDNFDYLTRDWSILGPHHLEEFVRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRLACKRLVSMNMPLNSDGTVCFNATLFALVRTNLKIYTEGNIDEANEQLRSAIKRIWKRTHKDLLDEVVPPAGKEDDVTVGKFYATFLIQDYFRRFKKRKEMEAKGVLPAQTPQAMALQAGLRTLHEIGPELKRAISGNLETDFNFDEPEPQHRRPHSLFNNLVHRLSGAGSKSPTEHERIEKGSTLLPFQPRSFSPTHSLAGAEGSPVPSQMHRGAPINQSINLPPVNGSARRLPALPPYANHIHDETDDGPRYRDTGDRAGYDQSSRMVVANRKYADARIVGAARREIEEAYSLGEQEIDLAADSLAPLMQHVGMHDIRDINENSRSALLRPAENSSRQHDSRGGSQEDLLLVTTL

Target ORF information:

RefSeq Version	NM_171379.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_171379.3

ORF Insert Sequence:

ATGTCAGTGT TAGCGAGTAT GATGTCATCG GGGGAGGACG AGGAGCAGGC GGCAGCTGAT 
GAGCAGGAGA GAACCGATCT GTGGCAGCAG ACATTACAGG CCGCCGTGGC AGCTTCATCA 
TCACAAGATG CCACAAAGAA ACGACCCGCA CAACGGAAAC CTCTTCGACA AACGAACGTC 
GTCGAAAGAT CCGAACGATC ACTTCTTTGC CTCTCCTTGA ATAATCCCAT CCGAAAATTG 
TGCATCAGTA TTGTTGAATG GAAACCATTC GAGTTTTTAA TATTGTTTAT GATATGTGCC 
AACTGTATCG CTCTAGCCAT CTATCAACCC TATCCTGCTC AAGATTCAGA CTATAAAAAT 
ACATTACTTG AAACAATTGA ATACGTGTTC ATTGTCGTAT TCACGATAGA ATGTGTGCTC 
AAAATAGTGG CTATGGGGTT TATGTTTCAT CCCAGTGCAT ATCTTCGAAA CGCATGGAAT 
ATTCTCGATT TTATTATTGT TGTCATAGGA CTTGTGAGTA CCATATTATC AAAAATGTCA 
ATTCAAGGAT TCGATGTAAA AGCTTTGAGA GCATTTCGTG TACTTCGACC GCTTCGTCTT 
GTATCAGGAG TACCAAGTTT ACAAGTTGTG CTCAACGCCA TCCTTCGAGC CATGATTCCT 
TTGCTCCACA TTGCCTTATT GGTTCTGTTT GTTATTTTGA TCTATGCTAT TATTGGATTA 
GAGTTATTCT GCGGAAAACT TCATTCAACA TGTATAGATC CAGCAACTGG ACAATTAGCG 
CAAAAAGATC CAACACCTTG TGGTACTGAT ACAGAAGGAA GTGCATTCAA ATGTCAACCA 
TCTGATTCTT TAACCAATAT GGGTGTTCGA TGGGAATGTT CAAGTAACAC AACATGGCCT 
GGTCCAAACA ATGGAATCAC GAACTTTGAT AACTTTGGAC TTGCTATGCT TACAGTATTC 
CAATGTGTAT CATTAGAAGG TTGGACAGAT GTAATGTACT GGGTAAATGA TGCTGTTGGC 
AGAGAGTGGC CGTGGATATA TTTTGTCACT CTTGTCATTC TTGGATCATT TTTCGTACTC 
AATCTTGTCT TGGGAGTCTT GTCTGGAGAA TTCTCAAAAG AACGAGAAAA GGCAAGAGCG 
AGAGGATTGT TTCAAAAATT TCGAGAAAAG CAACAACTTG AAGAAGATTT GAAAGGATAT 
CTCGACTGGA TCACACAAGC AGAAGATATT GAACCAGTGA ATGAAGATGA ACAAGAAGAT 
GAACCAGTTG CACAAACTGT AGTTGGAGAA GAAGCTGATG AAGAAGGAGA AGAACGAGTT 
GAAGATGTTA GACCGAGTAA ATGGGCAGCT AGAATGAAAC GATTAGAAAA ATTAAATAGG 
AGATGTCGAA GAGCTTGTAG AAGATTGGTC AAAAGTCAAA CATTTTATTG GCTGGTTATT 
CTTCTTGTGT TGCTCAATAC ATTAGTACTA ACATCTGAAC ATTATGGACA ATCCGAATGG 
CTCGATCATT TCCAAACAAT GGCAAACTTG TTCTTCGTAA TCCTTTTCTC GATGGAAATG 
CTCTTGAAAA TGTATTCGCT TGGGTTCACG ACTTACACGA CATCTCAATT TAATCGATTC 
GATTGTTTCG TCGTTATCAG TTCGATTTTG GAGTTTGTGC TTGTCTATTT CGATCTTATG 
AAGCCACTTG GAGTGTCTGT GCTGCGTTCA GCACGTCTTC TGAGAATATT CAAAGTTACA 
AAGTACTGGA CATCACTTCG TAATTTGGTA TCTTCACTTT TGAACTCTCT TCGATCCATC 
ATTTCCCTTC TGTTACTGCT TTTCCTGTTC ATTGTTATCT TTGCTTTATT GGGAATGCAA 
GTTTTCGGAG GAAAATTCAA TTTCAATCCA CAACAACCAA AACCAAGAGC CAATTTCGAT 
ACATTCGTTC AAGCATTGCT AACAGTATTC CAAATTCTTA CTGGTGAAGA TTGGAATACT 
GTAATGTATC ATGGAATTGA ATCATTTGGA GGTGTTGGAA CACTTGGAGT CATCGTCTGT 
ATCTACTATA TTGTTCTGTT CATTTGCGGT AATTACATTT TACTGAATGT CTTCTTGGCT 
ATTGCTGTCG ATAATTTGGC CGATGCAGAC AGTCTGACAA ATGCAGAAAA AGAAGAAGAG 
CAACAAGAAA TTGAAGGTGA AGATGAAGAA TTTGAGGAAG GAGAGGATGA AGGTGAAGAG 
CATGGAATGG ATGAACCAGA AGGAGATGAA GAAATGACAT CAGCAAGACC GAGAAGAATG 
AGTGAAGTTC CTGCTGCAAG TACGGTCAAA CCGATTCCAA AGGCTTCTTC GCTTTTCATT 
CTCAGTCATA CCAACTCTTT TCGAGTGTTT TGCAACATGG TTGTCAATCA TTCATATTTC 
ACCAACGCCG TTCTTTTTTG TATTTTGGTA TCATCTGCTA TGTTAGCTGC TGAAGATCCT 
CTACAAGCAA ACTCAACTAG AAATATGATT CTGAATTACT TTGACTACTT CTTCACGTCT 
GTGTTCACAG TTGAGATCAC GTTGAAAGTC ATCGTATTTG GTTTGGTATT CCACAAAGGA 
TCATTCTGTC GAAATGCATT CAATCTTCTC GATATCCTCG TCGTTGCAGT ATCATTAACA 
TCTTTTGTAC TTCGAACTGA TGCAATGTCT GTCGTCAAGA TTCTTCGTGT GCTCCGTGTG 
CTCCGTCCAC TTCGTGCAAT TAACCGAGCC AAGGGTTTGA AACATGTTGT ACAATGTGTA 
ATTGTTGCTG TAAAGACTAT CGGAAATATC ATGTTGGTCA CATTCATGCT TCAATTCATG 
TTTGCAATTA TCGGAGTTCA ACTTTTCAAG GGAACATTCT TCCTTTGCAA TGATCTTTCG 
AAAATGACAG AAGCAGAATG CCGTGGAGAA TATATTCATT ATGAAGATGG AGATCCAACA 
AAACCAGTTT CAAAGAAAAG AGTTTGGTCG AATAATGACT TCAATTTTGA TAACGTCGGA 
GATGCGATGA TTTCACTTTT CGTAGTTTCA ACTTTCGAAG GATGGCCACA ACTTCTTTAT 
GTTGCAATTG ATTCAAATGA AGAAGATAAG GGACCTATAC ATAATTCAAG ACAAGCTGTT 
GCTTTGTTCT TCATTGCTTT CATCATTGTC ATCGCATTCT TCATGATGAA CATCTTCGTA 
GGTTTCGTTA TCGTTACATT CCAAAATGAA GGAGAACGTG AATACGAGAA TTGTGAATTG 
GACAAGAATC AAAGAAAATG TATTGAATTT GCATTGAAAG CTAAACCACA CAGAAGATAC 
ATTCCACGAA ACAGATTACA ATACAGAGTT TGGTGGTTTG TCACATCAAG AGCTTTTGAG 
TATGTCATCT TTCTGATTAT CGTCATGAAT ACAGTATCTT TGGCGTGCAA GCATTATCCA 
AGTTCGAGAG GATTCGAAGA CTTTTTGGAT GTGTTCAATT TGATTTTCAC TGGAGTTTTC 
GCATTTGAAG CTGTTCTGAA GATTGTTGCA TTGAATCCGA AAAATTATAT TTCCGATCGA 
TGGAATGTCT TTGATTTGTT GGTTGTTGTC GGATCATTTA TTGATATCAC ATATGGAAAA 
TTGAATCCAG GAGGAACAAA TCTTATTTCA ATCAACTTTT TCCGTCTGTT CCGTGTGATG 
CGTCTCGTGA AGCTGCTTTC AAGAGGAGAA GGAATTCGAA CACTTTTATG GACATTCATG 
AAATCATTTC AAGCTCTTCC ATACGTCGCC TTGCTCATCG TTTTGCTCTT TTTCATTTAT 
GCTGTCATCG GAATGCAATT CTTTGGAAAA GTTGCACTGG ACGATTCAAC ATCTATACAC 
AGGAACAATA ACTTCCATTC GTTCCCTGCA GCGATTTTGG TGTTATTCAG ATCAGCAACT 
GGAGAAGCCT GGCAAGATAT CATGCTTTCG TGTTCGGATC GAGAAGATGT CAGATGTGAT 
CCAATGTCTG ATGATTACCA TAAAGGAGGC CTCAACGAAT CTAGATGTGG AAACAATTTT 
GCATATCCAT ACTTCATTTC ATTCTTCATG CTTTGCTCTT TCCTTGTCAT CAATCTTTTC 
GTAGCTGTTA TCATGGACAA TTTCGATTAT TTAACAAGAG ATTGGTCAAT TCTTGGGCCA 
CATCATTTGG AAGAATTTGT AAGATTATGG TCTGAATATG ATCCAGATGC AAAAGGACGA 
ATTAAACATC TTGACGTCGT CACACTTCTT CGAAAAATCT CTCCTCCACT TGGATTTGGA 
AAACTGTGTC CTCATCGATT GGCCTGTAAG AGATTGGTAT CAATGAATAT GCCCTTGAAC 
TCGGATGGTA CTGTATGCTT CAATGCAACT CTTTTTGCTC TCGTTCGTAC CAATTTGAAG 
ATTTATACTG AAGGAAACAT CGACGAAGCA AATGAACAGC TGCGATCAGC AATTAAACGA 
ATTTGGAAAC GAACTCATAA GGATTTGTTG GATGAGGTTG TTCCACCGGC AGGAAAAGAA 
GATGATGTGA CCGTTGGAAA ATTCTATGCC ACCTTCCTCA TTCAAGACTA TTTCCGTCGT 
TTCAAGAAGC GAAAAGAGAT GGAAGCAAAG GGTGTCTTAC CAGCTCAAAC ACCTCAAGCG 
ATGGCTCTTC AGGCCGGACT CCGCACGCTA CACGAGATCG GACCAGAATT GAAAAGAGCG 
ATTTCTGGAA ACTTGGAGAC CGACTTCAAC TTTGACGAAC CAGAGCCTCA ACACAGAAGA 
CCACACTCTC TTTTCAACAA TTTGGTACAT CGTCTATCTG GTGCCGGATC CAAATCTCCT 
ACTGAACACG AACGTATAGA AAAGGGCTCT ACACTTTTGC CATTCCAACC ACGCTCTTTC 
TCTCCAACTC ATTCACTTGC TGGCGCTGAA GGATCACCAG TACCATCACA AATGCATCGT 
GGAGCTCCAA TCAATCAATC AATAAATCTT CCACCTGTCA ATGGATCAGC TCGACGTCTT 
CCCGCCCTTC CGCCGTACGC AAATCATATT CACGATGAGA CGGACGACGG CCCCAGGTAT 
CGGGATACTG GCGATCGAGC TGGTTACGAT CAATCAAGCA GAATGGTTGT TGCCAATAGA 
AAGTACGCCG ACGCTCGGAT TGTCGGAGCC GCTCGACGAG AAATTGAGGA AGCATATTCA 
CTTGGTGAAC AGGAAATTGA CTTGGCAGCA GACTCATTAG CTCCATTAAT GCAACATGTT 
GGAATGCATG ATATCAGAGA TATCAATGAA AACTCTCGAA GCGCACTTTT ACGACCCGCC 
GAAAATTCAT CTCGGCAGCA TGATTCACGC GGAGGATCAC AAGAAGATCT ACTTTTAGTT 
ACAACTCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-19 ( NM_001027908.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001023079.1 Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), mRNA.

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CloneID	OCa343325
Clone ID Related Accession (Same CDS sequence)	NM_001027908.4 , NM_001027908.3
Accession Version	NM_001027908.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Voltage-dependent L-type calcium channel subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001027908.3.

ATGTCAGTGT TAGCGAGTAT GATGTCATCG GGGGAGGACG AGGAGCAGGC GGCAGCTGAT 
GAGCAGGAGA GAACCGATCT GTGGCAGCAG ACATTACAGG CCGCCGTGGC AGCTTCATCA 
TCACAAGATG CCACAAAGAA ACGACCCGCA CAACGGAAAC CTCTTCGACA AACGAACGTC 
GTCGAAAGAT CCGAACGATC ACTTCTTTGC CTCTCCTTGA ATAATCCCAT CCGAAAATTG 
TGCATCAGTA TTGTTGAATG GAAACCATTC GAGTTTTTAA TATTGTTTAT GATATGTGCC 
AACTGTATCG CTCTAGCCAT CTATCAACCC TATCCTGCTC AAGATTCAGA CTATAAAAAT 
ACATTACTTG AAACAATTGA ATACGTGTTC ATTGTCGTAT TCACGATAGA ATGTGTGCTC 
AAAATAGTGG CTATGGGGTT TATGTTTCAT CCCAGTGCAT ATCTTCGAAA CGCATGGAAT 
ATTCTCGATT TTATTATTGT TGTCATAGGA CTTGTGAGTA CCATATTATC AAAAATGTCA 
ATTCAAGGAT TCGATGTAAA AGCTTTGAGA GCATTTCGTG TACTTCGACC GCTTCGTCTT 
GTATCAGGAG TACCAAGTTT ACAAGTTGTG CTCAACGCCA TCCTTCGAGC CATGATTCCT 
TTGCTCCACA TTGCCTTATT GGTTCTGTTT GTTATTTTGA TCTATGCTAT TATTGGATTA 
GAGTTATTCT GCGGAAAACT TCATTCAACA TGTATAGATC CAGCAACTGG ACAATTAGCG 
CAAAAAGATC CAACACCTTG TGGTACTGAT ACAGAAGGAA GTGCATTCAA ATGTCAACCA 
TCTGATTCTT TAACCAATAT GGGTGTTCGA TGGGAATGTT CAAGTAACAC AACATGGCCT 
GGTCCAAACA ATGGAATCAC GAACTTTGAT AACTTTGGAC TTGCTATGCT TACAGTATTC 
CAATGTGTAT CATTAGAAGG TTGGACAGAT GTAATGTACT GGGTAAATGA TGCTGTTGGC 
AGAGAGTGGC CGTGGATATA TTTTGTCACT CTTGTCATTC TTGGATCATT TTTCGTACTC 
AATCTTGTCT TGGGAGTCTT GTCTGGAGAA TTCTCAAAAG AACGAGAAAA GGCAAGAGCG 
AGAGGATTGT TTCAAAAATT TCGAGAAAAG CAACAACTTG AAGAAGATTT GAAAGGATAT 
CTCGACTGGA TCACACAAGC AGAAGATATT GAACCAGTGA ATGAAGATGA ACAAGAAGAT 
GAACCAGTTG CACAAACTGT AGTTGGAGAA GAAGCTGATG AAGAAGGAGA AGAACGAGTT 
GAAGATGTTA GACCGAGTAA ATGGGCAGCT AGAATGAAAC GATTAGAAAA ATTAAATAGG 
AGATGTCGAA GAGCTTGTAG AAGATTGGTC AAAAGTCAAA CATTTTATTG GCTGGTTATT 
CTTCTTGTGT TGCTCAATAC ATTAGTACTA ACATCTGAAC ATTATGGACA ATCCGAATGG 
CTCGATCATT TCCAAACAAT GGCAAACTTG TTCTTCGTAA TCCTTTTCTC GATGGAAATG 
CTCTTGAAAA TGTATTCGCT TGGGTTCACG ACTTACACGA CATCTCAATT TAATCGATTC 
GATTGTTTCG TCGTTATCAG TTCGATTTTG GAGTTTGTGC TTGTCTATTT CGATCTTATG 
AAGCCACTTG GAGTGTCTGT GCTGCGTTCA GCACGTCTTC TGAGAATATT CAAAGTTACA 
AAGTACTGGA CATCACTTCG TAATTTGGTA TCTTCACTTT TGAACTCTCT TCGATCCATC 
ATTTCCCTTC TGTTACTGCT TTTCCTGTTC ATTGTTATCT TTGCTTTATT GGGAATGCAA 
GTTTTCGGAG GAAAATTCAA TTTCAATCCA CAACAACCAA AACCAAGAGC CAATTTCGAT 
ACATTCGTTC AAGCATTGCT AACAGTATTC CAAATTCTTA CTGGTGAAGA TTGGAATACT 
GTAATGTATC ATGGAATTGA ATCATTTGGA GGTGTTGGAA CACTTGGAGT CATCGTCTGT 
ATCTACTATA TTGTTCTGTT CATTTGCGGT AATTACATTT TACTGAATGT CTTCTTGGCT 
ATTGCTGTCG ATAATTTGGC CGATGCAGAC AGTCTGACAA ATGCAGAAAA AGAAGAAGAG 
CAACAAGAAA TTGAAGGTGA AGATGAAGAA TTTGAGGAAG GAGAGGATGA AGGTGAAGAG 
CATGGAATGG ATGAACCAGA AGGAGATGAA GAAATGACAT CAGCAAGACC GAGAAGAATG 
AGTGAAGTTC CTGCTGCAAG TACGGTCAAA CCGATTCCAA AGGCTTCTTC GCTTTTCATT 
CTCAGTCATA CCAACTCTTT TCGAGTGTTT TGCAACATGG TTGTCAATCA TTCATATTTC 
ACCAACGCCG TTCTTTTTTG TATTTTGGTA TCATCTGCTA TGTTAGCTGC TGAAGATCCT 
CTACAAGCAA ACTCAACTAG AAATATGATT CTGAATTACT TTGACTACTT CTTCACGTCT 
GTGTTCACAG TTGAGATCAC GTTGAAAGTC ATCGTATTTG GTTTGGTATT CCACAAAGGA 
TCATTCTGTC GAAATGCATT CAATCTTCTC GATATCCTCG TCGTTGCAGT ATCATTAACA 
TCTTTTGTAC TTCGAACTGA TGCAATGTCT GTCGTCAAGA TTCTTCGTGT GCTCCGTGTG 
CTCCGTCCAC TTCGTGCAAT TAACCGAGCC AAGGGTTTGA AACATGTTGT ACAATGTGTA 
ATTGTTGCTG TAAAGACTAT CGGAAATATC ATGTTGGTCA CATTCATGCT TCAATTCATG 
TTTGCAATTA TCGGAGTTCA ACTTTTCAAG GGAACATTCT TCCTTTGCAA TGATCTTTCG 
AAAATGACAG AAGCAGAATG CCGTGGAGAA TATATTCATT ATGAAGATGG AGATCCAACA 
AAACCAGTTT CAAAGAAAAG AGTTTGGTCG AATAATGACT TCAATTTTGA TAACGTCGGA 
GATGCGATGA TTTCACTTTT CGTAGTTTCA ACTTTCGAAG GATGGCCACA ACTTCTTTAT 
GTTGCAATTG ATTCAAATGA AGAAGATAAG GGACCTATAC ATAATTCAAG ACAAGCTGTT 
GCTTTGTTCT TCATTGCTTT CATCATTGTC ATCGCATTCT TCATGATGAA CATCTTCGTA 
GGTTTCGTTA TCGTTACATT CCAAAATGAA GGAGAACGTG AATACGAGAA TTGTGAATTG 
GACAAGAATC AAAGAAAATG TATTGAATTT GCATTGAAAG CTAAACCACA CAGAAGATAC 
ATTCCACGAA ACAGATTACA ATACAGAGTT TGGTGGTTTG TCACATCAAG AGCTTTTGAG 
TATGTCATCT TTCTGATTAT CGTCATGAAT ACAGTATCTT TGGCGTGCAA GCATTATCCA 
AGTTCGAGAG GATTCGAAGA CTTTTTGGAT GTGTTCAATT TGATTTTCAC TGGAGTTTTC 
GCATTTGAAG CTGTTCTGAA GATTGTTGCA TTGAATCCGA AAAATTATAT TTCCGATCGA 
TGGAATGTCT TTGATTTGTT GGTTGTTGTC GGATCATTTA TTGATATCAC ATATGGAAAA 
TTGAATCCAG GAGGAACAAA TCTTATTTCA ATCAACTTTT TCCGTCTGTT CCGTGTGATG 
CGTCTCGTGA AGCTGCTTTC AAGAGGAGAA GGAATTCGAA CACTTTTATG GACATTCATG 
AAATCATTTC AAGCTCTTCC ATACGTCGCC TTGCTCATCG TTTTGCTCTT TTTCATTTAT 
GCTGTCATCG GAATGCAATT CTTTGGAAAA GTTGCACTGG ACGATTCAAC ATCTATACAC 
AGGAACAATA ACTTCCATTC GTTCCCTGCA GCGATTTTGG TGTTATTCAG ATCAGCAACT 
GGAGAAGCCT GGCAAGATAT CATGCTTTCG TGTTCGGATC GAGAAGATGT CAGATGTGAT 
CCAATGTCTG ATGATTACCA TAAAGGAGGC CTCAACGAAT CTAGATGTGG AAACAATTTT 
GCATATCCAT ACTTCATTTC ATTCTTCATG CTTTGCTCTT TCCTTGTCAT CAATCTTTTC 
GTAGCTGTTA TCATGGACAA TTTCGATTAT TTAACAAGAG ATTGGTCAAT TCTTGGGCCA 
CATCATTTGG AAGAATTTGT AAGATTATGG TCTGAATATG ATCCAGATGC AAAAGGACGA 
ATTAAACATC TTGACGTCGT CACACTTCTT CGAAAAATCT CTCCTCCACT TGGATTTGGA 
AAACTGTGTC CTCATCGATT GGCCTGTAAG AGATTGGTAT CAATGAATAT GCCCTTGAAC 
TCGGATGGTA CTGTATGCTT CAATGCAACT CTTTTTGCTC TCGTTCGTAC CAATTTGAAG 
ATTTATACTG AAGGAAACAT CGACGAAGCA AATGAACAGC TGCGATCAGC AATTAAACGA 
ATTTGGAAAC GAACTCATAA GGATTTGTTG GATGAGGTTG TTCCACCGGC AGGAAAAGAA 
GATGATGTGA CCGTTGGAAA ATTCTATGCC ACCTTCCTCA TTCAAGACTA TTTCCGTCGT 
TTCAAGAAGC GAAAAGAGAT GGAAGCAAAG GGTGTCTTAC CAGCTCAAAC ACCTCAAGCG 
ATGGCTCTTC AGGCCGGACT CCGCACGCTA CACGAGATCG GACCAGAATT GAAAAGAGCG 
ATTTCTGGAA ACTTGGAGAC CGACTTCAAC TTTGACGAAC CAGAGCCTCA ACACAGAAGA 
CCACACTCTC TTTTCAACAA TTTGGTACAT CGTCTATCTG GTGCCGGATC CAAATCTCCT 
ACTGAACACG AACGTATAGA AAAGGGCTCT ACACTTTTGC CATTCCAACC ACGCTCTTTC 
TCTCCAACTC ATTCACTTGC TGGCGCTGAA GGATCACCAG TACCATCACA AATGCATCGT 
GGAGCTCCAA TCAATCAATC AATAAATCTT CCACCTGTCA ATGGATCAGC TCGACGTCTT 
CCCGCCCTTC CGCCGTACGC AAATCATATT CACGATGAGA CGGACGACGG CCCCAGGTAT 
CGGGATACTG GCGATCGAGC TGGTTACGAT CAATCAAGCA GAATGGTTGT TGCCAATAGA 
AATCTGCCTG TTGATCCCGA TGAAGAAGAG CAATGGATGA GAAGTGGTGG ACCGTCAAAT 
CGATCTGACA GACGAAACCC ACATCTACGA GAACCGATGC TTGTTGCTCG GGGAGCCGCT 
CTTGCACTCG CTGGAATGTC ATCAGAAGCC TACGAAGGTA CTTATAGGCC GGTTGGAGAA 
GGAAAGTCTG TTCGACTTCC ATTCTCATCT CGACCTGTTC TCCGACCTGC CGAAGATTCA 
CGACCTGTTG ATCGACTGAT CGGGCAATCA TTAGGACTTG GAAGGTACGC CGACGCTCGG 
ATTGTCGGAG CCGCTCGACG AGAAATTGAG GAAGCATATT CACTTGGTGA ACAGGAAATT 
GACTTGGCAG CAGACTCATT AGCTCCATTA ATGCAACATG TTGGAATGCA TGATATCAGA 
GATATCAATG AAAACTCTCG AAGCGCACTT TTACGACCCG CCGAAAATTC ATCTCGGCAG 
CATGATTCAC GCGGAGGATC ACAAGAAGAT CTACTTTTAG TTACAACTCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001023079.1
CDS	123..5756
Translation	MSVLASMMSSGEDEEQAAADEQERTDLWQQTLQAAVAASSSQDATKKRPAQRKPLRQTNVVERSERSLLCLSLNNPIRKLCISIVEWKPFEFLILFMICANCIALAIYQPYPAQDSDYKNTLLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNILDFIIVVIGLVSTILSKMSIQGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILRAMIPLLHIALLVLFVILIYAIIGLELFCGKLHSTCIDPATGQLAQKDPTPCGTDTEGSAFKCQPSDSLTNMGVRWECSSNTTWPGPNNGITNFDNFGLAMLTVFQCVSLEGWTDVMYWVNDAVGREWPWIYFVTLVILGSFFVLNLVLGVLSGEFSKEREKARARGLFQKFREKQQLEEDLKGYLDWITQAEDIEPVNEDEQEDEPVAQTVVGEEADEEGEERVEDVRPSKWAARMKRLEKLNRRCRRACRRLVKSQTFYWLVILLVLLNTLVLTSEHYGQSEWLDHFQTMANLFFVILFSMEMLLKMYSLGFTTYTTSQFNRFDCFVVISSILEFVLVYFDLMKPLGVSVLRSARLLRIFKVTKYWTSLRNLVSSLLNSLRSIISLLLLLFLFIVIFALLGMQVFGGKFNFNPQQPKPRANFDTFVQALLTVFQILTGEDWNTVMYHGIESFGGVGTLGVIVCIYYIVLFICGNYILLNVFLAIAVDNLADADSLTNAEKEEEQQEIEGEDEEFEEGEDEGEEHGMDEPEGDEEMTSARPRRMSEVPAASTVKPIPKASSLFILSHTNSFRVFCNMVVNHSYFTNAVLFCILVSSAMLAAEDPLQANSTRNMILNYFDYFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRTDAMSVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIMLVTFMLQFMFAIIGVQLFKGTFFLCNDLSKMTEAECRGEYIHYEDGDPTKPVSKKRVWSNNDFNFDNVGDAMISLFVVSTFEGWPQLLYVAIDSNEEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIFVGFVIVTFQNEGEREYENCELDKNQRKCIEFALKAKPHRRYIPRNRLQYRVWWFVTSRAFEYVIFLIIVMNTVSLACKHYPSSRGFEDFLDVFNLIFTGVFAFEAVLKIVALNPKNYISDRWNVFDLLVVVGSFIDITYGKLNPGGTNLISINFFRLFRVMRLVKLLSRGEGIRTLLWTFMKSFQALPYVALLIVLLFFIYAVIGMQFFGKVALDDSTSIHRNNNFHSFPAAILVLFRSATGEAWQDIMLSCSDREDVRCDPMSDDYHKGGLNESRCGNNFAYPYFISFFMLCSFLVINLFVAVIMDNFDYLTRDWSILGPHHLEEFVRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRLACKRLVSMNMPLNSDGTVCFNATLFALVRTNLKIYTEGNIDEANEQLRSAIKRIWKRTHKDLLDEVVPPAGKEDDVTVGKFYATFLIQDYFRRFKKRKEMEAKGVLPAQTPQAMALQAGLRTLHEIGPELKRAISGNLETDFNFDEPEPQHRRPHSLFNNLVHRLSGAGSKSPTEHERIEKGSTLLPFQPRSFSPTHSLAGAEGSPVPSQMHRGAPINQSINLPPVNGSARRLPALPPYANHIHDETDDGPRYRDTGDRAGYDQSSRMVVANRNLPVDPDEEEQWMRSGGPSNRSDRRNPHLREPMLVARGAALALAGMSSEAYEGTYRPVGEGKSVRLPFSSRPVLRPAEDSRPVDRLIGQSLGLGRYADARIVGAARREIEEAYSLGEQEIDLAADSLAPLMQHVGMHDIRDINENSRSALLRPAENSSRQHDSRGGSQEDLLLVTTL

Target ORF information:

RefSeq Version	NM_001027908.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027908.4

ORF Insert Sequence:

ATGTCAGTGT TAGCGAGTAT GATGTCATCG GGGGAGGACG AGGAGCAGGC GGCAGCTGAT 
GAGCAGGAGA GAACCGATCT GTGGCAGCAG ACATTACAGG CCGCCGTGGC AGCTTCATCA 
TCACAAGATG CCACAAAGAA ACGACCCGCA CAACGGAAAC CTCTTCGACA AACGAACGTC 
GTCGAAAGAT CCGAACGATC ACTTCTTTGC CTCTCCTTGA ATAATCCCAT CCGAAAATTG 
TGCATCAGTA TTGTTGAATG GAAACCATTC GAGTTTTTAA TATTGTTTAT GATATGTGCC 
AACTGTATCG CTCTAGCCAT CTATCAACCC TATCCTGCTC AAGATTCAGA CTATAAAAAT 
ACATTACTTG AAACAATTGA ATACGTGTTC ATTGTCGTAT TCACGATAGA ATGTGTGCTC 
AAAATAGTGG CTATGGGGTT TATGTTTCAT CCCAGTGCAT ATCTTCGAAA CGCATGGAAT 
ATTCTCGATT TTATTATTGT TGTCATAGGA CTTGTGAGTA CCATATTATC AAAAATGTCA 
ATTCAAGGAT TCGATGTAAA AGCTTTGAGA GCATTTCGTG TACTTCGACC GCTTCGTCTT 
GTATCAGGAG TACCAAGTTT ACAAGTTGTG CTCAACGCCA TCCTTCGAGC CATGATTCCT 
TTGCTCCACA TTGCCTTATT GGTTCTGTTT GTTATTTTGA TCTATGCTAT TATTGGATTA 
GAGTTATTCT GCGGAAAACT TCATTCAACA TGTATAGATC CAGCAACTGG ACAATTAGCG 
CAAAAAGATC CAACACCTTG TGGTACTGAT ACAGAAGGAA GTGCATTCAA ATGTCAACCA 
TCTGATTCTT TAACCAATAT GGGTGTTCGA TGGGAATGTT CAAGTAACAC AACATGGCCT 
GGTCCAAACA ATGGAATCAC GAACTTTGAT AACTTTGGAC TTGCTATGCT TACAGTATTC 
CAATGTGTAT CATTAGAAGG TTGGACAGAT GTAATGTACT GGGTAAATGA TGCTGTTGGC 
AGAGAGTGGC CGTGGATATA TTTTGTCACT CTTGTCATTC TTGGATCATT TTTCGTACTC 
AATCTTGTCT TGGGAGTCTT GTCTGGAGAA TTCTCAAAAG AACGAGAAAA GGCAAGAGCG 
AGAGGATTGT TTCAAAAATT TCGAGAAAAG CAACAACTTG AAGAAGATTT GAAAGGATAT 
CTCGACTGGA TCACACAAGC AGAAGATATT GAACCAGTGA ATGAAGATGA ACAAGAAGAT 
GAACCAGTTG CACAAACTGT AGTTGGAGAA GAAGCTGATG AAGAAGGAGA AGAACGAGTT 
GAAGATGTTA GACCGAGTAA ATGGGCAGCT AGAATGAAAC GATTAGAAAA ATTAAATAGG 
AGATGTCGAA GAGCTTGTAG AAGATTGGTC AAAAGTCAAA CATTTTATTG GCTGGTTATT 
CTTCTTGTGT TGCTCAATAC ATTAGTACTA ACATCTGAAC ATTATGGACA ATCCGAATGG 
CTCGATCATT TCCAAACAAT GGCAAACTTG TTCTTCGTAA TCCTTTTCTC GATGGAAATG 
CTCTTGAAAA TGTATTCGCT TGGGTTCACG ACTTACACGA CATCTCAATT TAATCGATTC 
GATTGTTTCG TCGTTATCAG TTCGATTTTG GAGTTTGTGC TTGTCTATTT CGATCTTATG 
AAGCCACTTG GAGTGTCTGT GCTGCGTTCA GCACGTCTTC TGAGAATATT CAAAGTTACA 
AAGTACTGGA CATCACTTCG TAATTTGGTA TCTTCACTTT TGAACTCTCT TCGATCCATC 
ATTTCCCTTC TGTTACTGCT TTTCCTGTTC ATTGTTATCT TTGCTTTATT GGGAATGCAA 
GTTTTCGGAG GAAAATTCAA TTTCAATCCA CAACAACCAA AACCAAGAGC CAATTTCGAT 
ACATTCGTTC AAGCATTGCT AACAGTATTC CAAATTCTTA CTGGTGAAGA TTGGAATACT 
GTAATGTATC ATGGAATTGA ATCATTTGGA GGTGTTGGAA CACTTGGAGT CATCGTCTGT 
ATCTACTATA TTGTTCTGTT CATTTGCGGT AATTACATTT TACTGAATGT CTTCTTGGCT 
ATTGCTGTCG ATAATTTGGC CGATGCAGAC AGTCTGACAA ATGCAGAAAA AGAAGAAGAG 
CAACAAGAAA TTGAAGGTGA AGATGAAGAA TTTGAGGAAG GAGAGGATGA AGGTGAAGAG 
CATGGAATGG ATGAACCAGA AGGAGATGAA GAAATGACAT CAGCAAGACC GAGAAGAATG 
AGTGAAGTTC CTGCTGCAAG TACGGTCAAA CCGATTCCAA AGGCTTCTTC GCTTTTCATT 
CTCAGTCATA CCAACTCTTT TCGAGTGTTT TGCAACATGG TTGTCAATCA TTCATATTTC 
ACCAACGCCG TTCTTTTTTG TATTTTGGTA TCATCTGCTA TGTTAGCTGC TGAAGATCCT 
CTACAAGCAA ACTCAACTAG AAATATGATT CTGAATTACT TTGACTACTT CTTCACGTCT 
GTGTTCACAG TTGAGATCAC GTTGAAAGTC ATCGTATTTG GTTTGGTATT CCACAAAGGA 
TCATTCTGTC GAAATGCATT CAATCTTCTC GATATCCTCG TCGTTGCAGT ATCATTAACA 
TCTTTTGTAC TTCGAACTGA TGCAATGTCT GTCGTCAAGA TTCTTCGTGT GCTCCGTGTG 
CTCCGTCCAC TTCGTGCAAT TAACCGAGCC AAGGGTTTGA AACATGTTGT ACAATGTGTA 
ATTGTTGCTG TAAAGACTAT CGGAAATATC ATGTTGGTCA CATTCATGCT TCAATTCATG 
TTTGCAATTA TCGGAGTTCA ACTTTTCAAG GGAACATTCT TCCTTTGCAA TGATCTTTCG 
AAAATGACAG AAGCAGAATG CCGTGGAGAA TATATTCATT ATGAAGATGG AGATCCAACA 
AAACCAGTTT CAAAGAAAAG AGTTTGGTCG AATAATGACT TCAATTTTGA TAACGTCGGA 
GATGCGATGA TTTCACTTTT CGTAGTTTCA ACTTTCGAAG GATGGCCACA ACTTCTTTAT 
GTTGCAATTG ATTCAAATGA AGAAGATAAG GGACCTATAC ATAATTCAAG ACAAGCTGTT 
GCTTTGTTCT TCATTGCTTT CATCATTGTC ATCGCATTCT TCATGATGAA CATCTTCGTA 
GGTTTCGTTA TCGTTACATT CCAAAATGAA GGAGAACGTG AATACGAGAA TTGTGAATTG 
GACAAGAATC AAAGAAAATG TATTGAATTT GCATTGAAAG CTAAACCACA CAGAAGATAC 
ATTCCACGAA ACAGATTACA ATACAGAGTT TGGTGGTTTG TCACATCAAG AGCTTTTGAG 
TATGTCATCT TTCTGATTAT CGTCATGAAT ACAGTATCTT TGGCGTGCAA GCATTATCCA 
AGTTCGAGAG GATTCGAAGA CTTTTTGGAT GTGTTCAATT TGATTTTCAC TGGAGTTTTC 
GCATTTGAAG CTGTTCTGAA GATTGTTGCA TTGAATCCGA AAAATTATAT TTCCGATCGA 
TGGAATGTCT TTGATTTGTT GGTTGTTGTC GGATCATTTA TTGATATCAC ATATGGAAAA 
TTGAATCCAG GAGGAACAAA TCTTATTTCA ATCAACTTTT TCCGTCTGTT CCGTGTGATG 
CGTCTCGTGA AGCTGCTTTC AAGAGGAGAA GGAATTCGAA CACTTTTATG GACATTCATG 
AAATCATTTC AAGCTCTTCC ATACGTCGCC TTGCTCATCG TTTTGCTCTT TTTCATTTAT 
GCTGTCATCG GAATGCAATT CTTTGGAAAA GTTGCACTGG ACGATTCAAC ATCTATACAC 
AGGAACAATA ACTTCCATTC GTTCCCTGCA GCGATTTTGG TGTTATTCAG ATCAGCAACT 
GGAGAAGCCT GGCAAGATAT CATGCTTTCG TGTTCGGATC GAGAAGATGT CAGATGTGAT 
CCAATGTCTG ATGATTACCA TAAAGGAGGC CTCAACGAAT CTAGATGTGG AAACAATTTT 
GCATATCCAT ACTTCATTTC ATTCTTCATG CTTTGCTCTT TCCTTGTCAT CAATCTTTTC 
GTAGCTGTTA TCATGGACAA TTTCGATTAT TTAACAAGAG ATTGGTCAAT TCTTGGGCCA 
CATCATTTGG AAGAATTTGT AAGATTATGG TCTGAATATG ATCCAGATGC AAAAGGACGA 
ATTAAACATC TTGACGTCGT CACACTTCTT CGAAAAATCT CTCCTCCACT TGGATTTGGA 
AAACTGTGTC CTCATCGATT GGCCTGTAAG AGATTGGTAT CAATGAATAT GCCCTTGAAC 
TCGGATGGTA CTGTATGCTT CAATGCAACT CTTTTTGCTC TCGTTCGTAC CAATTTGAAG 
ATTTATACTG AAGGAAACAT CGACGAAGCA AATGAACAGC TGCGATCAGC AATTAAACGA 
ATTTGGAAAC GAACTCATAA GGATTTGTTG GATGAGGTTG TTCCACCGGC AGGAAAAGAA 
GATGATGTGA CCGTTGGAAA ATTCTATGCC ACCTTCCTCA TTCAAGACTA TTTCCGTCGT 
TTCAAGAAGC GAAAAGAGAT GGAAGCAAAG GGTGTCTTAC CAGCTCAAAC ACCTCAAGCG 
ATGGCTCTTC AGGCCGGACT CCGCACGCTA CACGAGATCG GACCAGAATT GAAAAGAGCG 
ATTTCTGGAA ACTTGGAGAC CGACTTCAAC TTTGACGAAC CAGAGCCTCA ACACAGAAGA 
CCACACTCTC TTTTCAACAA TTTGGTACAT CGTCTATCTG GTGCCGGATC CAAATCTCCT 
ACTGAACACG AACGTATAGA AAAGGGCTCT ACACTTTTGC CATTCCAACC ACGCTCTTTC 
TCTCCAACTC ATTCACTTGC TGGCGCTGAA GGATCACCAG TACCATCACA AATGCATCGT 
GGAGCTCCAA TCAATCAATC AATAAATCTT CCACCTGTCA ATGGATCAGC TCGACGTCTT 
CCCGCCCTTC CGCCGTACGC AAATCATATT CACGATGAGA CGGACGACGG CCCCAGGTAT 
CGGGATACTG GCGATCGAGC TGGTTACGAT CAATCAAGCA GAATGGTTGT TGCCAATAGA 
AATCTGCCTG TTGATCCCGA TGAAGAAGAG CAATGGATGA GAAGTGGTGG ACCGTCAAAT 
CGATCTGACA GACGAAACCC ACATCTACGA GAACCGATGC TTGTTGCTCG GGGAGCCGCT 
CTTGCACTCG CTGGAATGTC ATCAGAAGCC TACGAAGGTA CTTATAGGCC GGTTGGAGAA 
GGAAAGTCTG TTCGACTTCC ATTCTCATCT CGACCTGTTC TCCGACCTGC CGAAGATTCA 
CGACCTGTTG ATCGACTGAT CGGGCAATCA TTAGGACTTG GAAGGTACGC CGACGCTCGG 
ATTGTCGGAG CCGCTCGACG AGAAATTGAG GAAGCATATT CACTTGGTGA ACAGGAAATT 
GACTTGGCAG CAGACTCATT AGCTCCATTA ATGCAACATG TTGGAATGCA TGATATCAGA 
GATATCAATG AAAACTCTCG AAGCGCACTT TTACGACCCG CCGAAAATTC ATCTCGGCAG 
CATGATTCAC GCGGAGGATC ACAAGAAGAT CTACTTTTAG TTACAACTCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl-19 ( NM_001027908.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001023079.1 Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), partial mRNA.

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CloneID	OCa343325
Clone ID Related Accession (Same CDS sequence)	NM_001027908.4 , NM_001027908.3
Accession Version	NM_001027908.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Voltage-dependent L-type calcium channel subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_001027908.2. COMPLETENESS: incomplete on both ends.

ATGTCAGTGT TAGCGAGTAT GATGTCATCG GGGGAGGACG AGGAGCAGGC GGCAGCTGAT 
GAGCAGGAGA GAACCGATCT GTGGCAGCAG ACATTACAGG CCGCCGTGGC AGCTTCATCA 
TCACAAGATG CCACAAAGAA ACGACCCGCA CAACGGAAAC CTCTTCGACA AACGAACGTC 
GTCGAAAGAT CCGAACGATC ACTTCTTTGC CTCTCCTTGA ATAATCCCAT CCGAAAATTG 
TGCATCAGTA TTGTTGAATG GAAACCATTC GAGTTTTTAA TATTGTTTAT GATATGTGCC 
AACTGTATCG CTCTAGCCAT CTATCAACCC TATCCTGCTC AAGATTCAGA CTATAAAAAT 
ACATTACTTG AAACAATTGA ATACGTGTTC ATTGTCGTAT TCACGATAGA ATGTGTGCTC 
AAAATAGTGG CTATGGGGTT TATGTTTCAT CCCAGTGCAT ATCTTCGAAA CGCATGGAAT 
ATTCTCGATT TTATTATTGT TGTCATAGGA CTTGTGAGTA CCATATTATC AAAAATGTCA 
ATTCAAGGAT TCGATGTAAA AGCTTTGAGA GCATTTCGTG TACTTCGACC GCTTCGTCTT 
GTATCAGGAG TACCAAGTTT ACAAGTTGTG CTCAACGCCA TCCTTCGAGC CATGATTCCT 
TTGCTCCACA TTGCCTTATT GGTTCTGTTT GTTATTTTGA TCTATGCTAT TATTGGATTA 
GAGTTATTCT GCGGAAAACT TCATTCAACA TGTATAGATC CAGCAACTGG ACAATTAGCG 
CAAAAAGATC CAACACCTTG TGGTACTGAT ACAGAAGGAA GTGCATTCAA ATGTCAACCA 
TCTGATTCTT TAACCAATAT GGGTGTTCGA TGGGAATGTT CAAGTAACAC AACATGGCCT 
GGTCCAAACA ATGGAATCAC GAACTTTGAT AACTTTGGAC TTGCTATGCT TACAGTATTC 
CAATGTGTAT CATTAGAAGG TTGGACAGAT GTAATGTACT GGGTAAATGA TGCTGTTGGC 
AGAGAGTGGC CGTGGATATA TTTTGTCACT CTTGTCATTC TTGGATCATT TTTCGTACTC 
AATCTTGTCT TGGGAGTCTT GTCTGGAGAA TTCTCAAAAG AACGAGAAAA GGCAAGAGCG 
AGAGGATTGT TTCAAAAATT TCGAGAAAAG CAACAACTTG AAGAAGATTT GAAAGGATAT 
CTCGACTGGA TCACACAAGC AGAAGATATT GAACCAGTGA ATGAAGATGA ACAAGAAGAT 
GAACCAGTTG CACAAACTGT AGTTGGAGAA GAAGCTGATG AAGAAGGAGA AGAACGAGTT 
GAAGATGTTA GACCGAGTAA ATGGGCAGCT AGAATGAAAC GATTAGAAAA ATTAAATAGG 
AGATGTCGAA GAGCTTGTAG AAGATTGGTC AAAAGTCAAA CATTTTATTG GCTGGTTATT 
CTTCTTGTGT TGCTCAATAC ATTAGTACTA ACATCTGAAC ATTATGGACA ATCCGAATGG 
CTCGATCATT TCCAAACAAT GGCAAACTTG TTCTTCGTAA TCCTTTTCTC GATGGAAATG 
CTCTTGAAAA TGTATTCGCT TGGGTTCACG ACTTACACGA CATCTCAATT TAATCGATTC 
GATTGTTTCG TCGTTATCAG TTCGATTTTG GAGTTTGTGC TTGTCTATTT CGATCTTATG 
AAGCCACTTG GAGTGTCTGT GCTGCGTTCA GCACGTCTTC TGAGAATATT CAAAGTTACA 
AAGTACTGGA CATCACTTCG TAATTTGGTA TCTTCACTTT TGAACTCTCT TCGATCCATC 
ATTTCCCTTC TGTTACTGCT TTTCCTGTTC ATTGTTATCT TTGCTTTATT GGGAATGCAA 
GTTTTCGGAG GAAAATTCAA TTTCAATCCA CAACAACCAA AACCAAGAGC CAATTTCGAT 
ACATTCGTTC AAGCATTGCT AACAGTATTC CAAATTCTTA CTGGTGAAGA TTGGAATACT 
GTAATGTATC ATGGAATTGA ATCATTTGGA GGTGTTGGAA CACTTGGAGT CATCGTCTGT 
ATCTACTATA TTGTTCTGTT CATTTGCGGT AATTACATTT TACTGAATGT CTTCTTGGCT 
ATTGCTGTCG ATAATTTGGC CGATGCAGAC AGTCTGACAA ATGCAGAAAA AGAAGAAGAG 
CAACAAGAAA TTGAAGGTGA AGATGAAGAA TTTGAGGAAG GAGAGGATGA AGGTGAAGAG 
CATGGAATGG ATGAACCAGA AGGAGATGAA GAAATGACAT CAGCAAGACC GAGAAGAATG 
AGTGAAGTTC CTGCTGCAAG TACGGTCAAA CCGATTCCAA AGGCTTCTTC GCTTTTCATT 
CTCAGTCATA CCAACTCTTT TCGAGTGTTT TGCAACATGG TTGTCAATCA TTCATATTTC 
ACCAACGCCG TTCTTTTTTG TATTTTGGTA TCATCTGCTA TGTTAGCTGC TGAAGATCCT 
CTACAAGCAA ACTCAACTAG AAATATGATT CTGAATTACT TTGACTACTT CTTCACGTCT 
GTGTTCACAG TTGAGATCAC GTTGAAAGTC ATCGTATTTG GTTTGGTATT CCACAAAGGA 
TCATTCTGTC GAAATGCATT CAATCTTCTC GATATCCTCG TCGTTGCAGT ATCATTAACA 
TCTTTTGTAC TTCGAACTGA TGCAATGTCT GTCGTCAAGA TTCTTCGTGT GCTCCGTGTG 
CTCCGTCCAC TTCGTGCAAT TAACCGAGCC AAGGGTTTGA AACATGTTGT ACAATGTGTA 
ATTGTTGCTG TAAAGACTAT CGGAAATATC ATGTTGGTCA CATTCATGCT TCAATTCATG 
TTTGCAATTA TCGGAGTTCA ACTTTTCAAG GGAACATTCT TCCTTTGCAA TGATCTTTCG 
AAAATGACAG AAGCAGAATG CCGTGGAGAA TATATTCATT ATGAAGATGG AGATCCAACA 
AAACCAGTTT CAAAGAAAAG AGTTTGGTCG AATAATGACT TCAATTTTGA TAACGTCGGA 
GATGCGATGA TTTCACTTTT CGTAGTTTCA ACTTTCGAAG GATGGCCACA ACTTCTTTAT 
GTTGCAATTG ATTCAAATGA AGAAGATAAG GGACCTATAC ATAATTCAAG ACAAGCTGTT 
GCTTTGTTCT TCATTGCTTT CATCATTGTC ATCGCATTCT TCATGATGAA CATCTTCGTA 
GGTTTCGTTA TCGTTACATT CCAAAATGAA GGAGAACGTG AATACGAGAA TTGTGAATTG 
GACAAGAATC AAAGAAAATG TATTGAATTT GCATTGAAAG CTAAACCACA CAGAAGATAC 
ATTCCACGAA ACAGATTACA ATACAGAGTT TGGTGGTTTG TCACATCAAG AGCTTTTGAG 
TATGTCATCT TTCTGATTAT CGTCATGAAT ACAGTATCTT TGGCGTGCAA GCATTATCCA 
AGTTCGAGAG GATTCGAAGA CTTTTTGGAT GTGTTCAATT TGATTTTCAC TGGAGTTTTC 
GCATTTGAAG CTGTTCTGAA GATTGTTGCA TTGAATCCGA AAAATTATAT TTCCGATCGA 
TGGAATGTCT TTGATTTGTT GGTTGTTGTC GGATCATTTA TTGATATCAC ATATGGAAAA 
TTGAATCCAG GAGGAACAAA TCTTATTTCA ATCAACTTTT TCCGTCTGTT CCGTGTGATG 
CGTCTCGTGA AGCTGCTTTC AAGAGGAGAA GGAATTCGAA CACTTTTATG GACATTCATG 
AAATCATTTC AAGCTCTTCC ATACGTCGCC TTGCTCATCG TTTTGCTCTT TTTCATTTAT 
GCTGTCATCG GAATGCAATT CTTTGGAAAA GTTGCACTGG ACGATTCAAC ATCTATACAC 
AGGAACAATA ACTTCCATTC GTTCCCTGCA GCGATTTTGG TGTTATTCAG ATCAGCAACT 
GGAGAAGCCT GGCAAGATAT CATGCTTTCG TGTTCGGATC GAGAAGATGT CAGATGTGAT 
CCAATGTCTG ATGATTACCA TAAAGGAGGC CTCAACGAAT CTAGATGTGG AAACAATTTT 
GCATATCCAT ACTTCATTTC ATTCTTCATG CTTTGCTCTT TCCTTGTCAT CAATCTTTTC 
GTAGCTGTTA TCATGGACAA TTTCGATTAT TTAACAAGAG ATTGGTCAAT TCTTGGGCCA 
CATCATTTGG AAGAATTTGT AAGATTATGG TCTGAATATG ATCCAGATGC AAAAGGACGA 
ATTAAACATC TTGACGTCGT CACACTTCTT CGAAAAATCT CTCCTCCACT TGGATTTGGA 
AAACTGTGTC CTCATCGATT GGCCTGTAAG AGATTGGTAT CAATGAATAT GCCCTTGAAC 
TCGGATGGTA CTGTATGCTT CAATGCAACT CTTTTTGCTC TCGTTCGTAC CAATTTGAAG 
ATTTATACTG AAGGAAACAT CGACGAAGCA AATGAACAGC TGCGATCAGC AATTAAACGA 
ATTTGGAAAC GAACTCATAA GGATTTGTTG GATGAGGTTG TTCCACCGGC AGGAAAAGAA 
GATGATGTGA CCGTTGGAAA ATTCTATGCC ACCTTCCTCA TTCAAGACTA TTTCCGTCGT 
TTCAAGAAGC GAAAAGAGAT GGAAGCAAAG GGTGTCTTAC CAGCTCAAAC ACCTCAAGCG 
ATGGCTCTTC AGGCCGGACT CCGCACGCTA CACGAGATCG GACCAGAATT GAAAAGAGCG 
ATTTCTGGAA ACTTGGAGAC CGACTTCAAC TTTGACGAAC CAGAGCCTCA ACACAGAAGA 
CCACACTCTC TTTTCAACAA TTTGGTACAT CGTCTATCTG GTGCCGGATC CAAATCTCCT 
ACTGAACACG AACGTATAGA AAAGGGCTCT ACACTTTTGC CATTCCAACC ACGCTCTTTC 
TCTCCAACTC ATTCACTTGC TGGCGCTGAA GGATCACCAG TACCATCACA AATGCATCGT 
GGAGCTCCAA TCAATCAATC AATAAATCTT CCACCTGTCA ATGGATCAGC TCGACGTCTT 
CCCGCCCTTC CGCCGTACGC AAATCATATT CACGATGAGA CGGACGACGG CCCCAGGTAT 
CGGGATACTG GCGATCGAGC TGGTTACGAT CAATCAAGCA GAATGGTTGT TGCCAATAGA 
AATCTGCCTG TTGATCCCGA TGAAGAAGAG CAATGGATGA GAAGTGGTGG ACCGTCAAAT 
CGATCTGACA GACGAAACCC ACATCTACGA GAACCGATGC TTGTTGCTCG GGGAGCCGCT 
CTTGCACTCG CTGGAATGTC ATCAGAAGCC TACGAAGGTA CTTATAGGCC GGTTGGAGAA 
GGAAAGTCTG TTCGACTTCC ATTCTCATCT CGACCTGTTC TCCGACCTGC CGAAGATTCA 
CGACCTGTTG ATCGACTGAT CGGGCAATCA TTAGGACTTG GAAGGTACGC CGACGCTCGG 
ATTGTCGGAG CCGCTCGACG AGAAATTGAG GAAGCATATT CACTTGGTGA ACAGGAAATT 
GACTTGGCAG CAGACTCATT AGCTCCATTA ATGCAACATG TTGGAATGCA TGATATCAGA 
GATATCAATG AAAACTCTCG AAGCGCACTT TTACGACCCG CCGAAAATTC ATCTCGGCAG 
CATGATTCAC GCGGAGGATC ACAAGAAGAT CTACTTTTAG TTACAACTCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001023079.1
CDS	1..5634
Translation	MSVLASMMSSGEDEEQAAADEQERTDLWQQTLQAAVAASSSQDATKKRPAQRKPLRQTNVVERSERSLLCLSLNNPIRKLCISIVEWKPFEFLILFMICANCIALAIYQPYPAQDSDYKNTLLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNILDFIIVVIGLVSTILSKMSIQGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILRAMIPLLHIALLVLFVILIYAIIGLELFCGKLHSTCIDPATGQLAQKDPTPCGTDTEGSAFKCQPSDSLTNMGVRWECSSNTTWPGPNNGITNFDNFGLAMLTVFQCVSLEGWTDVMYWVNDAVGREWPWIYFVTLVILGSFFVLNLVLGVLSGEFSKEREKARARGLFQKFREKQQLEEDLKGYLDWITQAEDIEPVNEDEQEDEPVAQTVVGEEADEEGEERVEDVRPSKWAARMKRLEKLNRRCRRACRRLVKSQTFYWLVILLVLLNTLVLTSEHYGQSEWLDHFQTMANLFFVILFSMEMLLKMYSLGFTTYTTSQFNRFDCFVVISSILEFVLVYFDLMKPLGVSVLRSARLLRIFKVTKYWTSLRNLVSSLLNSLRSIISLLLLLFLFIVIFALLGMQVFGGKFNFNPQQPKPRANFDTFVQALLTVFQILTGEDWNTVMYHGIESFGGVGTLGVIVCIYYIVLFICGNYILLNVFLAIAVDNLADADSLTNAEKEEEQQEIEGEDEEFEEGEDEGEEHGMDEPEGDEEMTSARPRRMSEVPAASTVKPIPKASSLFILSHTNSFRVFCNMVVNHSYFTNAVLFCILVSSAMLAAEDPLQANSTRNMILNYFDYFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRTDAMSVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIMLVTFMLQFMFAIIGVQLFKGTFFLCNDLSKMTEAECRGEYIHYEDGDPTKPVSKKRVWSNNDFNFDNVGDAMISLFVVSTFEGWPQLLYVAIDSNEEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIFVGFVIVTFQNEGEREYENCELDKNQRKCIEFALKAKPHRRYIPRNRLQYRVWWFVTSRAFEYVIFLIIVMNTVSLACKHYPSSRGFEDFLDVFNLIFTGVFAFEAVLKIVALNPKNYISDRWNVFDLLVVVGSFIDITYGKLNPGGTNLISINFFRLFRVMRLVKLLSRGEGIRTLLWTFMKSFQALPYVALLIVLLFFIYAVIGMQFFGKVALDDSTSIHRNNNFHSFPAAILVLFRSATGEAWQDIMLSCSDREDVRCDPMSDDYHKGGLNESRCGNNFAYPYFISFFMLCSFLVINLFVAVIMDNFDYLTRDWSILGPHHLEEFVRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRLACKRLVSMNMPLNSDGTVCFNATLFALVRTNLKIYTEGNIDEANEQLRSAIKRIWKRTHKDLLDEVVPPAGKEDDVTVGKFYATFLIQDYFRRFKKRKEMEAKGVLPAQTPQAMALQAGLRTLHEIGPELKRAISGNLETDFNFDEPEPQHRRPHSLFNNLVHRLSGAGSKSPTEHERIEKGSTLLPFQPRSFSPTHSLAGAEGSPVPSQMHRGAPINQSINLPPVNGSARRLPALPPYANHIHDETDDGPRYRDTGDRAGYDQSSRMVVANRNLPVDPDEEEQWMRSGGPSNRSDRRNPHLREPMLVARGAALALAGMSSEAYEGTYRPVGEGKSVRLPFSSRPVLRPAEDSRPVDRLIGQSLGLGRYADARIVGAARREIEEAYSLGEQEIDLAADSLAPLMQHVGMHDIRDINENSRSALLRPAENSSRQHDSRGGSQEDLLLVTTL

Target ORF information:

RefSeq Version	NM_001027908.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Voltage-dependent L-type calcium channel subunit alpha (egl-19), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027908.3

ORF Insert Sequence:

ATGTCAGTGT TAGCGAGTAT GATGTCATCG GGGGAGGACG AGGAGCAGGC GGCAGCTGAT 
GAGCAGGAGA GAACCGATCT GTGGCAGCAG ACATTACAGG CCGCCGTGGC AGCTTCATCA 
TCACAAGATG CCACAAAGAA ACGACCCGCA CAACGGAAAC CTCTTCGACA AACGAACGTC 
GTCGAAAGAT CCGAACGATC ACTTCTTTGC CTCTCCTTGA ATAATCCCAT CCGAAAATTG 
TGCATCAGTA TTGTTGAATG GAAACCATTC GAGTTTTTAA TATTGTTTAT GATATGTGCC 
AACTGTATCG CTCTAGCCAT CTATCAACCC TATCCTGCTC AAGATTCAGA CTATAAAAAT 
ACATTACTTG AAACAATTGA ATACGTGTTC ATTGTCGTAT TCACGATAGA ATGTGTGCTC 
AAAATAGTGG CTATGGGGTT TATGTTTCAT CCCAGTGCAT ATCTTCGAAA CGCATGGAAT 
ATTCTCGATT TTATTATTGT TGTCATAGGA CTTGTGAGTA CCATATTATC AAAAATGTCA 
ATTCAAGGAT TCGATGTAAA AGCTTTGAGA GCATTTCGTG TACTTCGACC GCTTCGTCTT 
GTATCAGGAG TACCAAGTTT ACAAGTTGTG CTCAACGCCA TCCTTCGAGC CATGATTCCT 
TTGCTCCACA TTGCCTTATT GGTTCTGTTT GTTATTTTGA TCTATGCTAT TATTGGATTA 
GAGTTATTCT GCGGAAAACT TCATTCAACA TGTATAGATC CAGCAACTGG ACAATTAGCG 
CAAAAAGATC CAACACCTTG TGGTACTGAT ACAGAAGGAA GTGCATTCAA ATGTCAACCA 
TCTGATTCTT TAACCAATAT GGGTGTTCGA TGGGAATGTT CAAGTAACAC AACATGGCCT 
GGTCCAAACA ATGGAATCAC GAACTTTGAT AACTTTGGAC TTGCTATGCT TACAGTATTC 
CAATGTGTAT CATTAGAAGG TTGGACAGAT GTAATGTACT GGGTAAATGA TGCTGTTGGC 
AGAGAGTGGC CGTGGATATA TTTTGTCACT CTTGTCATTC TTGGATCATT TTTCGTACTC 
AATCTTGTCT TGGGAGTCTT GTCTGGAGAA TTCTCAAAAG AACGAGAAAA GGCAAGAGCG 
AGAGGATTGT TTCAAAAATT TCGAGAAAAG CAACAACTTG AAGAAGATTT GAAAGGATAT 
CTCGACTGGA TCACACAAGC AGAAGATATT GAACCAGTGA ATGAAGATGA ACAAGAAGAT 
GAACCAGTTG CACAAACTGT AGTTGGAGAA GAAGCTGATG AAGAAGGAGA AGAACGAGTT 
GAAGATGTTA GACCGAGTAA ATGGGCAGCT AGAATGAAAC GATTAGAAAA ATTAAATAGG 
AGATGTCGAA GAGCTTGTAG AAGATTGGTC AAAAGTCAAA CATTTTATTG GCTGGTTATT 
CTTCTTGTGT TGCTCAATAC ATTAGTACTA ACATCTGAAC ATTATGGACA ATCCGAATGG 
CTCGATCATT TCCAAACAAT GGCAAACTTG TTCTTCGTAA TCCTTTTCTC GATGGAAATG 
CTCTTGAAAA TGTATTCGCT TGGGTTCACG ACTTACACGA CATCTCAATT TAATCGATTC 
GATTGTTTCG TCGTTATCAG TTCGATTTTG GAGTTTGTGC TTGTCTATTT CGATCTTATG 
AAGCCACTTG GAGTGTCTGT GCTGCGTTCA GCACGTCTTC TGAGAATATT CAAAGTTACA 
AAGTACTGGA CATCACTTCG TAATTTGGTA TCTTCACTTT TGAACTCTCT TCGATCCATC 
ATTTCCCTTC TGTTACTGCT TTTCCTGTTC ATTGTTATCT TTGCTTTATT GGGAATGCAA 
GTTTTCGGAG GAAAATTCAA TTTCAATCCA CAACAACCAA AACCAAGAGC CAATTTCGAT 
ACATTCGTTC AAGCATTGCT AACAGTATTC CAAATTCTTA CTGGTGAAGA TTGGAATACT 
GTAATGTATC ATGGAATTGA ATCATTTGGA GGTGTTGGAA CACTTGGAGT CATCGTCTGT 
ATCTACTATA TTGTTCTGTT CATTTGCGGT AATTACATTT TACTGAATGT CTTCTTGGCT 
ATTGCTGTCG ATAATTTGGC CGATGCAGAC AGTCTGACAA ATGCAGAAAA AGAAGAAGAG 
CAACAAGAAA TTGAAGGTGA AGATGAAGAA TTTGAGGAAG GAGAGGATGA AGGTGAAGAG 
CATGGAATGG ATGAACCAGA AGGAGATGAA GAAATGACAT CAGCAAGACC GAGAAGAATG 
AGTGAAGTTC CTGCTGCAAG TACGGTCAAA CCGATTCCAA AGGCTTCTTC GCTTTTCATT 
CTCAGTCATA CCAACTCTTT TCGAGTGTTT TGCAACATGG TTGTCAATCA TTCATATTTC 
ACCAACGCCG TTCTTTTTTG TATTTTGGTA TCATCTGCTA TGTTAGCTGC TGAAGATCCT 
CTACAAGCAA ACTCAACTAG AAATATGATT CTGAATTACT TTGACTACTT CTTCACGTCT 
GTGTTCACAG TTGAGATCAC GTTGAAAGTC ATCGTATTTG GTTTGGTATT CCACAAAGGA 
TCATTCTGTC GAAATGCATT CAATCTTCTC GATATCCTCG TCGTTGCAGT ATCATTAACA 
TCTTTTGTAC TTCGAACTGA TGCAATGTCT GTCGTCAAGA TTCTTCGTGT GCTCCGTGTG 
CTCCGTCCAC TTCGTGCAAT TAACCGAGCC AAGGGTTTGA AACATGTTGT ACAATGTGTA 
ATTGTTGCTG TAAAGACTAT CGGAAATATC ATGTTGGTCA CATTCATGCT TCAATTCATG 
TTTGCAATTA TCGGAGTTCA ACTTTTCAAG GGAACATTCT TCCTTTGCAA TGATCTTTCG 
AAAATGACAG AAGCAGAATG CCGTGGAGAA TATATTCATT ATGAAGATGG AGATCCAACA 
AAACCAGTTT CAAAGAAAAG AGTTTGGTCG AATAATGACT TCAATTTTGA TAACGTCGGA 
GATGCGATGA TTTCACTTTT CGTAGTTTCA ACTTTCGAAG GATGGCCACA ACTTCTTTAT 
GTTGCAATTG ATTCAAATGA AGAAGATAAG GGACCTATAC ATAATTCAAG ACAAGCTGTT 
GCTTTGTTCT TCATTGCTTT CATCATTGTC ATCGCATTCT TCATGATGAA CATCTTCGTA 
GGTTTCGTTA TCGTTACATT CCAAAATGAA GGAGAACGTG AATACGAGAA TTGTGAATTG 
GACAAGAATC AAAGAAAATG TATTGAATTT GCATTGAAAG CTAAACCACA CAGAAGATAC 
ATTCCACGAA ACAGATTACA ATACAGAGTT TGGTGGTTTG TCACATCAAG AGCTTTTGAG 
TATGTCATCT TTCTGATTAT CGTCATGAAT ACAGTATCTT TGGCGTGCAA GCATTATCCA 
AGTTCGAGAG GATTCGAAGA CTTTTTGGAT GTGTTCAATT TGATTTTCAC TGGAGTTTTC 
GCATTTGAAG CTGTTCTGAA GATTGTTGCA TTGAATCCGA AAAATTATAT TTCCGATCGA 
TGGAATGTCT TTGATTTGTT GGTTGTTGTC GGATCATTTA TTGATATCAC ATATGGAAAA 
TTGAATCCAG GAGGAACAAA TCTTATTTCA ATCAACTTTT TCCGTCTGTT CCGTGTGATG 
CGTCTCGTGA AGCTGCTTTC AAGAGGAGAA GGAATTCGAA CACTTTTATG GACATTCATG 
AAATCATTTC AAGCTCTTCC ATACGTCGCC TTGCTCATCG TTTTGCTCTT TTTCATTTAT 
GCTGTCATCG GAATGCAATT CTTTGGAAAA GTTGCACTGG ACGATTCAAC ATCTATACAC 
AGGAACAATA ACTTCCATTC GTTCCCTGCA GCGATTTTGG TGTTATTCAG ATCAGCAACT 
GGAGAAGCCT GGCAAGATAT CATGCTTTCG TGTTCGGATC GAGAAGATGT CAGATGTGAT 
CCAATGTCTG ATGATTACCA TAAAGGAGGC CTCAACGAAT CTAGATGTGG AAACAATTTT 
GCATATCCAT ACTTCATTTC ATTCTTCATG CTTTGCTCTT TCCTTGTCAT CAATCTTTTC 
GTAGCTGTTA TCATGGACAA TTTCGATTAT TTAACAAGAG ATTGGTCAAT TCTTGGGCCA 
CATCATTTGG AAGAATTTGT AAGATTATGG TCTGAATATG ATCCAGATGC AAAAGGACGA 
ATTAAACATC TTGACGTCGT CACACTTCTT CGAAAAATCT CTCCTCCACT TGGATTTGGA 
AAACTGTGTC CTCATCGATT GGCCTGTAAG AGATTGGTAT CAATGAATAT GCCCTTGAAC 
TCGGATGGTA CTGTATGCTT CAATGCAACT CTTTTTGCTC TCGTTCGTAC CAATTTGAAG 
ATTTATACTG AAGGAAACAT CGACGAAGCA AATGAACAGC TGCGATCAGC AATTAAACGA 
ATTTGGAAAC GAACTCATAA GGATTTGTTG GATGAGGTTG TTCCACCGGC AGGAAAAGAA 
GATGATGTGA CCGTTGGAAA ATTCTATGCC ACCTTCCTCA TTCAAGACTA TTTCCGTCGT 
TTCAAGAAGC GAAAAGAGAT GGAAGCAAAG GGTGTCTTAC CAGCTCAAAC ACCTCAAGCG 
ATGGCTCTTC AGGCCGGACT CCGCACGCTA CACGAGATCG GACCAGAATT GAAAAGAGCG 
ATTTCTGGAA ACTTGGAGAC CGACTTCAAC TTTGACGAAC CAGAGCCTCA ACACAGAAGA 
CCACACTCTC TTTTCAACAA TTTGGTACAT CGTCTATCTG GTGCCGGATC CAAATCTCCT 
ACTGAACACG AACGTATAGA AAAGGGCTCT ACACTTTTGC CATTCCAACC ACGCTCTTTC 
TCTCCAACTC ATTCACTTGC TGGCGCTGAA GGATCACCAG TACCATCACA AATGCATCGT 
GGAGCTCCAA TCAATCAATC AATAAATCTT CCACCTGTCA ATGGATCAGC TCGACGTCTT 
CCCGCCCTTC CGCCGTACGC AAATCATATT CACGATGAGA CGGACGACGG CCCCAGGTAT 
CGGGATACTG GCGATCGAGC TGGTTACGAT CAATCAAGCA GAATGGTTGT TGCCAATAGA 
AATCTGCCTG TTGATCCCGA TGAAGAAGAG CAATGGATGA GAAGTGGTGG ACCGTCAAAT 
CGATCTGACA GACGAAACCC ACATCTACGA GAACCGATGC TTGTTGCTCG GGGAGCCGCT 
CTTGCACTCG CTGGAATGTC ATCAGAAGCC TACGAAGGTA CTTATAGGCC GGTTGGAGAA 
GGAAAGTCTG TTCGACTTCC ATTCTCATCT CGACCTGTTC TCCGACCTGC CGAAGATTCA 
CGACCTGTTG ATCGACTGAT CGGGCAATCA TTAGGACTTG GAAGGTACGC CGACGCTCGG 
ATTGTCGGAG CCGCTCGACG AGAAATTGAG GAAGCATATT CACTTGGTGA ACAGGAAATT 
GACTTGGCAG CAGACTCATT AGCTCCATTA ATGCAACATG TTGGAATGCA TGATATCAGA 
GATATCAATG AAAACTCTCG AAGCGCACTT TTACGACCCG CCGAAAATTC ATCTCGGCAG 
CATGATTCAC GCGGAGGATC ACAAGAAGAT CTACTTTTAG TTACAACTCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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egl-19 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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