CACNA1D cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1D
Entrez Gene ID	408013
Full Name	calcium voltage-gated channel subunit alpha1 D
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 D Names voltage-dependent L-type calcium channel subunit alpha-1D voltage-gated calcium channel alpha 1D subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010817672.1	XP_010815974.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
XM_015459540.1	XP_015315026.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
XM_015459542.1	XP_015315028.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X17
XM_010817681.1	XP_010815983.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X20
XM_010817674.2	XP_010815976.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
XM_005222722.4	XP_005222779.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
XM_010817680.2	XP_010815982.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
XM_010817673.1	XP_010815975.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
XM_010817676.1	XP_010815978.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
XM_010817679.1	XP_010815981.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X18
XM_005222720.3	XP_005222777.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
XM_005222722.3	XP_005222779.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
XM_010817678.2	XP_010815980.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
XM_005222720.4	XP_005222777.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
XM_015459537.1	XP_015315023.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
XM_010817670.1	XP_010815972.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
XM_010817671.1	XP_010815973.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
XM_010817678.1	XP_010815980.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
XM_005222721.3	XP_005222778.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
XM_024982436.1	XP_024838204.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
NM_001193025.1	NP_001179954.1	voltage-dependent L-type calcium channel subunit alpha-1D
XM_015459538.1	XP_015315024.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
XM_010817680.1	XP_010815982.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X19
XM_010817676.2	XP_010815978.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
XM_010817674.1	XP_010815976.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
XM_010817677.1	XP_010815979.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
XM_015459541.1	XP_015315027.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
XM_015459539.1	XP_015315025.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
XM_010817677.2	XP_010815979.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CACNA1D	NP_001179954.1
Rattus norvegicus (Norway rat)	Cacna1d	NP_058994.1
Gallus gallus (chicken)	CACNA1D	NP_990365.1
Homo sapiens (human)	CACNA1D	NP_000711.1
Pan troglodytes (chimpanzee)	CACNA1D	XP_001173076.3
	CACNA1D	XP_002802833.1
Mus musculus (house mouse)	Cacna1d	NP_001077085.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012434	XP_001231059.2
Canis lupus familiaris (dog)	CACNA1D	XP_849431.1
Danio rerio (zebrafish)	cacna1da	NP_982351.1
Drosophila melanogaster (fruit fly)	Ca-alpha1D	NP_001260484.1
Caenorhabditis elegans (roundworm)	egl-19	NP_001023079.1

Gene Ontology
Bibliography

Related articles in PubMed

Adrenal fasciculata cells express T-type and rapidly and slowly activating L-type Ca2+ channels that regulate cortisol secretion.
Enyeart JJ, Enyeart JA
American journal of physiology. Cell physiology308(11)C899-918(2015 Jun)

Expression profile of the pore-forming subunits ?1A and ?1D in the foetal bovine hypothalamus: a mammal with a long gestation.
Peruffo A, Giacomello M, Montelli S, Panin M, Cozzi B
Neuroscience letters556124-8(2013 Nov)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Adrenal medulla calcium channel population is not conserved in bovine chromaffin cells in culture.
Benavides A, Calvo S, Tornero D, Gonz?lez-Garc?a C, Ce?a V
Neuroscience128(1)99-109(2004)

Differential expression of calcium channel subtypes in the bovine adrenal medulla.
Garc?a-Palomero E, Renart J, Andr?s-Mateos E, Sol?s-Garrido LM, Matute C, Herrero CJ, Garc?a AG, Montiel C
Neuroendocrinology74(4)251-61(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

new information on the hypothalamic expression of alpha1A and alpha1D subunits during development in a mammal with a long gestation period and a large and convoluted brain.
Title: Expression profile of the pore-forming subunits ?1A and ?1D in the foetal bovine hypothalamus: a mammal with a long gestation.

The following CACNA1D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa94404	XM_015459540.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94405	XM_015459541.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94406	XM_015459542.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa228749	XM_024982436.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa01213	NM_001193025.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa31497	XM_005222720.4 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa31498	XM_005222721.3 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa31499	XM_005222722.4 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45767	XM_010817670.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45768	XM_010817671.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45769	XM_010817672.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45770	XM_010817673.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45771	XM_010817674.2 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45773	XM_010817676.2 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45774	XM_010817677.2 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45775	XM_010817678.2 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45776	XM_010817679.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45777	XM_010817680.2 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45778	XM_010817681.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa94401	XM_015459537.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa94402	XM_015459538.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa94403	XM_015459539.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
OBa31499	XM_005222722.3	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa31497	XM_005222720.3	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45775	XM_010817678.1	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45777	XM_010817680.1	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45773	XM_010817676.1	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45771	XM_010817674.1	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa45774	XM_010817677.1	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1D ( XM_015459540.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315026.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X15, mRNA.

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CloneID	OBa94404
Clone ID Related Accession (Same CDS sequence)	XM_015459540.1
Accession Version	XM_015459540.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAAGAAAGC AAGTTCCCAG CTCAACAAAT GCCAATCTCA 
ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG AACCTTGAGC 
TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT CACAGAAGGT 
GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA GGAGACGAGC 
AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG GACCCCCACT 
GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC AGCTCGCCCA 
CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC AGCAGCTTGG 
ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC GACGACTCGC 
CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC ACCCCTACAT 
CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC CAGGAGGAGG 
TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315026.1
CDS	259..6069
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESESVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKESKFPAQQMPISIMPICAKLPLESGPALGTLSLCLKTGIIPSTSMAVSLTEGAVLKEPAIMKLTLGLTQETSSSRRFAGRTRRYTATSGTPTAQGSRSISAARSTMRMTARPPGAGKATAAATITGTWAAAWTLSGLEATITPKASQRTTTRPLATIPGGLRGDAYYLPPLHPPAGLPSTSSACAGRAARRRSRRRPPSRPARPCLCT

Target ORF information:

RefSeq Version	XM_015459540.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459540.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAAGAAAGC AAGTTCCCAG CTCAACAAAT GCCAATCTCA 
ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG AACCTTGAGC 
TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT CACAGAAGGT 
GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA GGAGACGAGC 
AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG GACCCCCACT 
GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC AGCTCGCCCA 
CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC AGCAGCTTGG 
ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC GACGACTCGC 
CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC ACCCCTACAT 
CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC CAGGAGGAGG 
TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015459541.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315027.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X16, mRNA.

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CloneID	OBa94405
Clone ID Related Accession (Same CDS sequence)	XM_015459541.1
Accession Version	XM_015459541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAAGAAAGC AAGTTCCCAG CTCAACAAAT GCCAATCTCA 
ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG AACCTTGAGC 
TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT CACAGAAGGT 
GCAGTATTAA AAGGTCTGAC TCAGGAGACG AGCAGTTCCC GACGATTTGC CGGGAGGACC 
CGGAGATACA CGGCTACTTC AGGGACCCCC ACTGCCCAGG GGAGCAGGAG TATTTCAGCA 
GCGAGGAGTA CTATGAGGAT GACAGCTCGC CCACCGGGAG CAGGCAAAGC TACGGCTGCG 
GCTACTATAA CTGGTACCTG GGCAGCAGCT TGGACTTTGA GTGGCCTCGA GGCTACCATC 
ACCCCCAAAG CTTCTCAGAG GACGACGACT CGCCCGTTGG CTACGATTCC CGGAGGTCTC 
CGAGGCGACG CCTACTACCT CCCACCCCTA CATCCCCCCG CAGGTCTTCC TTCAACTTCG 
AGTGCCTGCG CCGGCAGAGC AGCCAGGAGG AGGTCCCGCC GTCGCCCGCC TTCCCGCCCC 
GCACGGCCCT GCCTCTGCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315027.1
CDS	259..6042
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESESVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKESKFPAQQMPISIMPICAKLPLESGPALGTLSLCLKTGIIPSTSMAVSLTEGAVLKGLTQETSSSRRFAGRTRRYTATSGTPTAQGSRSISAARSTMRMTARPPGAGKATAAATITGTWAAAWTLSGLEATITPKASQRTTTRPLATIPGGLRGDAYYLPPLHPPAGLPSTSSACAGRAARRRSRRRPPSRPARPCLCT

Target ORF information:

RefSeq Version	XM_015459541.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459541.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAAGAAAGC AAGTTCCCAG CTCAACAAAT GCCAATCTCA 
ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG AACCTTGAGC 
TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT CACAGAAGGT 
GCAGTATTAA AAGGTCTGAC TCAGGAGACG AGCAGTTCCC GACGATTTGC CGGGAGGACC 
CGGAGATACA CGGCTACTTC AGGGACCCCC ACTGCCCAGG GGAGCAGGAG TATTTCAGCA 
GCGAGGAGTA CTATGAGGAT GACAGCTCGC CCACCGGGAG CAGGCAAAGC TACGGCTGCG 
GCTACTATAA CTGGTACCTG GGCAGCAGCT TGGACTTTGA GTGGCCTCGA GGCTACCATC 
ACCCCCAAAG CTTCTCAGAG GACGACGACT CGCCCGTTGG CTACGATTCC CGGAGGTCTC 
CGAGGCGACG CCTACTACCT CCCACCCCTA CATCCCCCCG CAGGTCTTCC TTCAACTTCG 
AGTGCCTGCG CCGGCAGAGC AGCCAGGAGG AGGTCCCGCC GTCGCCCGCC TTCCCGCCCC 
GCACGGCCCT GCCTCTGCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015459542.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315028.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X17, mRNA.

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CloneID	OBa94406
Clone ID Related Accession (Same CDS sequence)	XM_015459542.1
Accession Version	XM_015459542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAACAGGAA ATTCGGTGTG TCATAACCAT CATAACCATA 
ATTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315028.1
CDS	259..5487
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESESVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKQEIRCVITIITIIP

Target ORF information:

RefSeq Version	XM_015459542.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459542.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAACAGGAA ATTCGGTGTG TCATAACCAT CATAACCATA 
ATTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_024982436.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838204.1 Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X6, mRNA.

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CloneID	OBa228749
Clone ID Related Accession (Same CDS sequence)	XM_024982436.1
Accession Version	XM_024982436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGGTCTGAC TCAGGAGACG AGCAGTTCCC GACGATTTGC 
CGGGAGGACC CGGAGATACA CGGCTACTTC AGGGACCCCC ACTGCCCAGG GGAGCAGGAG 
TATTTCAGCA GCGAGGAGTA CTATGAGGAT GACAGCTCGC CCACCGGGAG CAGGCAAAGC 
TACGGCTGCG GCTACTATAA CTGGTACCTG GGCAGCAGCT TGGACTTTGA GTGGCCTCGA 
GGCTACCATC ACCCCCAAAG CTTCTCAGAG GACGACGACT CGCCCGTTGG CTACGATTCC 
CGGAGGTCTC CGAGGCGACG CCTACTACCT CCCACCCCTA CATCCCCCCG CAGGTCTTCC 
TTCAACTTCG AGTGCCTGCG CCGGCAGAGC AGCCAGGAGG AGGTCCCGCC GTCGCCCGCC 
TTCCCGCCCC GCACGGCCCT GCCTCTGCAC CTGATGCAGC AGCAGATCAT GGCAGTCGCC 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCGCCGAGCC ACTCCACCCG GTCGTGGGCC 
ACGCCCCCGG CCACGCCGTC GTATCGGGAC TGGACGCCGT GCTATACCCC GCTGATCCAG 
GTTGAGCGGC CAGAGGCCCC AGACCAGGTC AACGGCAGCC TCCCGTCCCT GCACCGCAGC 
TCGTGGTACA CGGACGAGCC CAGCGTCTCC TATCGGACGT TCACACCAGC CAGCCTGACC 
GTCCCCAGCA GCTTCCACAA CAAGAACAGC GACAAGCAGA GGAGCGCGGA CAGCCTGGTG 
GAGGCAGTTC TGATATCCGA AGGCTTAGGA CGGTACGCGA GGGACCCAAA GTTCGTGTCG 
GCGACAAAAC ACGAAATCGC CGATGCCTGT GACTTAACCA TCGACGAGAT GGAGAGTGCG 
GCCAGCAACC TGCTGAACGG GAACGTGCAT CCGCGGGCCA ATGGAGACGT GGGCCCCGTT 
TCCCACCGGC AAGACTACGA GCTCCAGGAC TTTGGGCCTG GCTACAGCGA CGAGGAGCCG 
GACCCCGGGA GGGACGAGGA GGACCTGGCA GACGAGATGA TTTGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838204.1
CDS	374..6793
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_024982436.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982436.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGGTCTGAC TCAGGAGACG AGCAGTTCCC GACGATTTGC 
CGGGAGGACC CGGAGATACA CGGCTACTTC AGGGACCCCC ACTGCCCAGG GGAGCAGGAG 
TATTTCAGCA GCGAGGAGTA CTATGAGGAT GACAGCTCGC CCACCGGGAG CAGGCAAAGC 
TACGGCTGCG GCTACTATAA CTGGTACCTG GGCAGCAGCT TGGACTTTGA GTGGCCTCGA 
GGCTACCATC ACCCCCAAAG CTTCTCAGAG GACGACGACT CGCCCGTTGG CTACGATTCC 
CGGAGGTCTC CGAGGCGACG CCTACTACCT CCCACCCCTA CATCCCCCCG CAGGTCTTCC 
TTCAACTTCG AGTGCCTGCG CCGGCAGAGC AGCCAGGAGG AGGTCCCGCC GTCGCCCGCC 
TTCCCGCCCC GCACGGCCCT GCCTCTGCAC CTGATGCAGC AGCAGATCAT GGCAGTCGCC 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCGCCGAGCC ACTCCACCCG GTCGTGGGCC 
ACGCCCCCGG CCACGCCGTC GTATCGGGAC TGGACGCCGT GCTATACCCC GCTGATCCAG 
GTTGAGCGGC CAGAGGCCCC AGACCAGGTC AACGGCAGCC TCCCGTCCCT GCACCGCAGC 
TCGTGGTACA CGGACGAGCC CAGCGTCTCC TATCGGACGT TCACACCAGC CAGCCTGACC 
GTCCCCAGCA GCTTCCACAA CAAGAACAGC GACAAGCAGA GGAGCGCGGA CAGCCTGGTG 
GAGGCAGTTC TGATATCCGA AGGCTTAGGA CGGTACGCGA GGGACCCAAA GTTCGTGTCG 
GCGACAAAAC ACGAAATCGC CGATGCCTGT GACTTAACCA TCGACGAGAT GGAGAGTGCG 
GCCAGCAACC TGCTGAACGG GAACGTGCAT CCGCGGGCCA ATGGAGACGT GGGCCCCGTT 
TCCCACCGGC AAGACTACGA GCTCCAGGAC TTTGGGCCTG GCTACAGCGA CGAGGAGCCG 
GACCCCGGGA GGGACGAGGA GGACCTGGCA GACGAGATGA TTTGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( NM_001193025.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179954.1 Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), mRNA.

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CloneID	OBa01213
Clone ID Related Accession (Same CDS sequence)	NM_001193025.1
Accession Version	NM_001193025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000022.1. On Jul 17, 2010 this sequence version replaced XM_865676.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CCAACTGAAA GTGAAAGTGT CCCTGTCCCA 
ACCGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACGCTGCTG 
TGGACTTTTA TTAAGTCCTT CCAGGCGCTC CCGTATGTCG CCCTTCTTAT CGCCATGCTG 
TTCTTCATCT ACGCGGTCAT CGGCATGCAG ATGTTCGGGA AAGTGGCCAT GAGAGATAAC 
AACCAGATCA ACAGAAACAA CAATTTCCAG ACCTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCGA CGGGGGAGGC CTGGCAGGAA ATCATGTTAG CCTGCCTCCC GGGGAAGCAA 
TGTGACCCCG ACTCGGATTA CAACCCCGGG GAGGAATACA CATGTGGGAG CAACTTCGCC 
ATCGTTTATT TCATCAGTTT TTATATGCTC TGCGCATTTC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGATTATCTC ACCCGGGACT GGTCTATTCT GGGGCCTCAC 
CATTTAGATG AATTCAAACG GATCTGGTCT GAATATGACC CCGAGGCAAA GGGCAGGATC 
AAGCACCTGG ACGTGGTCAC TCTGCTCCGC CGCATCCAGC CTCCCCTGGG CTTCGGGAAG 
TTGTGTCCAC ACCGGGTAGC CTGCAAGAGA CTAGTTGCTA TGAACATGCC ACTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACTCTG TTTGCTCTGG TTCGAACGGC TCTTAAAATC 
AAGACTGAAG GCAACCTGGA ACAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ACTACTTGAC CAAGTTGTTC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCCGCGA AGAACAGCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGC 
GATTTGCAAG ATGATGATCC AGAGGAAACA AAACGAGAAG ACGAAGAAGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT GCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCTTGTG 
TCTGAAAACG GGCATCATTC CTTCCACAAG CATGGCCGTG AGCCTCACAG AAGGTGCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCTG ACTCAGGAGA CGAGCAGTTC 
CCGACGATTT GCCGGGAGGA CCCGGAGATA CACGGCTACT TCAGGGACCC CCACTGCCCA 
GGGGAGCAGG AGTATTTCAG CAGCGAGGAG TACTATGAGG ATGACAGCTC GCCCACCGGG 
AGCAGGCAAA GCTACGGCTG CGGCTACTAT AACTGGTACC TGGGCAGCAG CTTGGACTTT 
GAGTGGCCTC GAGGCTACCA TCACCCCCAA AGCTTCTCAG AGGACGACGA CTCGCCCGTT 
GGCTACGATT CCCGGAGGTC TCCGAGGCGA CGCCTACTAC CTCCCACCCC TACATCCCCC 
CGCAGGTCTT CCTTCAACTT CGAGTGCCTG CGCCGGCAGA GCAGCCAGGA GGAGGTCCCG 
CCGTCGCCCG CCTTCCCGCC CCGCACGGCC CTGCCTCTGC ACCTGATGCA GCAGCAGATC 
ATGGCAGTCG CCGGCCTAGA TTCCAGTAAA GCCCAGAAGT ACTCGCCGAG CCACTCCACC 
CGGTCGTGGG CCACGCCCCC GGCCACGCCG TCGTATCGGG ACTGGACGCC GTGCTATACC 
CCGCTGATCC AGGTTGAGCG GCCAGAGGCC CCAGACCAGG TCAACGGCAG CCTCCCGTCC 
CTGCACCGCA GCTCGTGGTA CACGGACGAG CCCAGCGTCT CCTATCGGAC GTTCACACCA 
GCCAGCCTGA CCGTCCCCAG CAGCTTCCAC AACAAGAACA GCGACAAGCA GAGGAGCGCG 
GACAGCCTGG TGGAGGCAGT TCTGATATCC GAAGGCTTAG GACGGTACGC GAGGGACCCA 
AAGTTCGTGT CGGCGACAAA ACACGAAATC GCCGATGCCT GTGACTTAAC CATCGACGAG 
ATGGAGAGTG CGGCCAGCAA CCTGCTGAAC GGGAACGTGC ATCCGCGGGC CAATGGAGAC 
GTGGGCCCCG TTTCCCACCG GCAAGACTAC GAGCTCCAGG ACTTTGGGCC TGGCTACAGC 
GACGAGGAGC CGGACCCCGG GAGGGACGAG GAGGACCTGG CAGACGAGAT GATTTGCATC 
ACCACCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179954.1
CDS	1..6552
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADPTESESVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	NM_001193025.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193025.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CCAACTGAAA GTGAAAGTGT CCCTGTCCCA 
ACCGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACGCTGCTG 
TGGACTTTTA TTAAGTCCTT CCAGGCGCTC CCGTATGTCG CCCTTCTTAT CGCCATGCTG 
TTCTTCATCT ACGCGGTCAT CGGCATGCAG ATGTTCGGGA AAGTGGCCAT GAGAGATAAC 
AACCAGATCA ACAGAAACAA CAATTTCCAG ACCTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCGA CGGGGGAGGC CTGGCAGGAA ATCATGTTAG CCTGCCTCCC GGGGAAGCAA 
TGTGACCCCG ACTCGGATTA CAACCCCGGG GAGGAATACA CATGTGGGAG CAACTTCGCC 
ATCGTTTATT TCATCAGTTT TTATATGCTC TGCGCATTTC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGATTATCTC ACCCGGGACT GGTCTATTCT GGGGCCTCAC 
CATTTAGATG AATTCAAACG GATCTGGTCT GAATATGACC CCGAGGCAAA GGGCAGGATC 
AAGCACCTGG ACGTGGTCAC TCTGCTCCGC CGCATCCAGC CTCCCCTGGG CTTCGGGAAG 
TTGTGTCCAC ACCGGGTAGC CTGCAAGAGA CTAGTTGCTA TGAACATGCC ACTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACTCTG TTTGCTCTGG TTCGAACGGC TCTTAAAATC 
AAGACTGAAG GCAACCTGGA ACAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ACTACTTGAC CAAGTTGTTC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCCGCGA AGAACAGCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGC 
GATTTGCAAG ATGATGATCC AGAGGAAACA AAACGAGAAG ACGAAGAAGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT GCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCTTGTG 
TCTGAAAACG GGCATCATTC CTTCCACAAG CATGGCCGTG AGCCTCACAG AAGGTGCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCTG ACTCAGGAGA CGAGCAGTTC 
CCGACGATTT GCCGGGAGGA CCCGGAGATA CACGGCTACT TCAGGGACCC CCACTGCCCA 
GGGGAGCAGG AGTATTTCAG CAGCGAGGAG TACTATGAGG ATGACAGCTC GCCCACCGGG 
AGCAGGCAAA GCTACGGCTG CGGCTACTAT AACTGGTACC TGGGCAGCAG CTTGGACTTT 
GAGTGGCCTC GAGGCTACCA TCACCCCCAA AGCTTCTCAG AGGACGACGA CTCGCCCGTT 
GGCTACGATT CCCGGAGGTC TCCGAGGCGA CGCCTACTAC CTCCCACCCC TACATCCCCC 
CGCAGGTCTT CCTTCAACTT CGAGTGCCTG CGCCGGCAGA GCAGCCAGGA GGAGGTCCCG 
CCGTCGCCCG CCTTCCCGCC CCGCACGGCC CTGCCTCTGC ACCTGATGCA GCAGCAGATC 
ATGGCAGTCG CCGGCCTAGA TTCCAGTAAA GCCCAGAAGT ACTCGCCGAG CCACTCCACC 
CGGTCGTGGG CCACGCCCCC GGCCACGCCG TCGTATCGGG ACTGGACGCC GTGCTATACC 
CCGCTGATCC AGGTTGAGCG GCCAGAGGCC CCAGACCAGG TCAACGGCAG CCTCCCGTCC 
CTGCACCGCA GCTCGTGGTA CACGGACGAG CCCAGCGTCT CCTATCGGAC GTTCACACCA 
GCCAGCCTGA CCGTCCCCAG CAGCTTCCAC AACAAGAACA GCGACAAGCA GAGGAGCGCG 
GACAGCCTGG TGGAGGCAGT TCTGATATCC GAAGGCTTAG GACGGTACGC GAGGGACCCA 
AAGTTCGTGT CGGCGACAAA ACACGAAATC GCCGATGCCT GTGACTTAAC CATCGACGAG 
ATGGAGAGTG CGGCCAGCAA CCTGCTGAAC GGGAACGTGC ATCCGCGGGC CAATGGAGAC 
GTGGGCCCCG TTTCCCACCG GCAAGACTAC GAGCTCCAGG ACTTTGGGCC TGGCTACAGC 
GACGAGGAGC CGGACCCCGG GAGGGACGAG GAGGACCTGG CAGACGAGAT GATTTGCATC 
ACCACCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_005222720.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222777.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X11, mRNA.

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CloneID	OBa31497
Clone ID Related Accession (Same CDS sequence)	XM_005222720.3 , XM_005222720.4
Accession Version	XM_005222720.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CCAACTGAAA GTGAAAGTGT CCCTGTCCCA 
ACCGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACGCTGCTG 
TGGACTTTTA TTAAGTCCTT CCAGGCGCTC CCGTATGTCG CCCTTCTTAT CGCCATGCTG 
TTCTTCATCT ACGCGGTCAT CGGCATGCAG ATGTTCGGGA AAGTGGCCAT GAGAGATAAC 
AACCAGATCA ACAGAAACAA CAATTTCCAG ACCTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCGA CGGGGGAGGC CTGGCAGGAA ATCATGTTAG CCTGCCTCCC GGGGAAGCAA 
TGTGACCCCG ACTCGGATTA CAACCCCGGG GAGGAATACA CATGTGGGAG CAACTTCGCC 
ATCGTTTATT TCATCAGTTT TTATATGCTC TGCGCATTTC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGATTATCTC ACCCGGGACT GGTCTATTCT GGGGCCTCAC 
CATTTAGATG AATTCAAACG GATCTGGTCT GAATATGACC CCGAGGCAAA GGGCAGGATC 
AAGCACCTGG ACGTGGTCAC TCTGCTCCGC CGCATCCAGC CTCCCCTGGG CTTCGGGAAG 
TTGTGTCCAC ACCGGGTAGC CTGCAAGAGA CTAGTTGCTA TGAACATGCC ACTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACTCTG TTTGCTCTGG TTCGAACGGC TCTTAAAATC 
AAGACTGAAG GCAACCTGGA ACAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ACTACTTGAC CAAGTTGTTC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCCGCGA AGAACAGCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGC 
GATTTGCAAG ATGATGATCC AGAGGAAACA AAACGAGAAG ACGAAGAAGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT GCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCTTGTG 
TCTGAAAACG GGCATCATTC CTTCCACAAG CATGGCCGTG AGCCTCACAG AAGGTGCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCTG ACTCAGGAGA CGAGCAGTTC 
CCGACGATTT GCCGGGAGGA CCCGGAGATA CACGGCTACT TCAGGGACCC CCACTGCCCA 
GGGGAGCAGG AGTATTTCAG CAGCGAGGAG TACTATGAGG ATGACAGCTC GCCCACCGGG 
AGCAGGCAAA GCTACGGCTG CGGCTACTAT AACTGGTACC TGGGCAGCAG CTTGGACTTT 
GAGTGGCCTC GAGGCTACCA TCACCCCCAA AGCTTCTCAG AGGACGACGA CTCGCCCGTT 
GGCTACGATT CCCGGAGGTC TCCGAGGCGA CGCCTACTAC CTCCCACCCC TACATCCCCC 
CGCAGGTCTT CCTTCAACTT CGAGTGCCTG CGCCGGCAGA GCAGCCAGGA GGAGGTCCCG 
CCGTCGCCCG CCTTCCCGCC CCGCACGGCC CTGCCTCTGC ACCTGATGCA GCAGCAGATC 
ATGGCAGTCG CCGGCCTAGA TTCCAGTAAA GCCCAGAAGT ACTCGCCGAG CCACTCCACC 
CGGTCGTGGG CCACGCCCCC GGCCACGCCG TCGTATCGGG ACTGGACGCC GTGCTATACC 
CCGCTGATCC AGGTTGAGCG GCCAGAGGCC CCAGACCAGG TCAACGGCAG CCTCCCGTCC 
CTGCACCGCA GCTCGTGGTA CACGGACGAG CCCAGCGTCT CCTATCGGAC GTTCACACCA 
GCCAGCCTGA CCGTCCCCAG CAGCTTCCAC AACAAGAACA GCGACAAGCA GAGGAGCGCG 
GACAGCCTGG TGGAGGCAGT TCTGATATCC GAAGGCTTAG GACGGTACGC GAGGGACCCA 
AAGTTCGTGT CGGCGACAAA ACACGAAATC GCCGATGCCT GTGACTTAAC CATCGACGAG 
ATGGAGAGTG CGGCCAGCAA CCTGCTGAAC GGGAACGTGC ATCCGCGGGC CAATGGAGAC 
GTGGGCCCCG TTTCCCACCG GCAAGACTAC GAGCTCCAGG ACTTTGGGCC TGGCTACAGC 
GACGAGGAGC CGGACCCCGG GAGGGACGAG GAGGACCTGG CAGACGAGAT GATTTGCATC 
ACCACCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222777.1
CDS	1..6492
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADPTESESVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_005222720.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222720.3

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CCAACTGAAA GTGAAAGTGT CCCTGTCCCA 
ACCGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACGCTGCTG 
TGGACTTTTA TTAAGTCCTT CCAGGCGCTC CCGTATGTCG CCCTTCTTAT CGCCATGCTG 
TTCTTCATCT ACGCGGTCAT CGGCATGCAG ATGTTCGGGA AAGTGGCCAT GAGAGATAAC 
AACCAGATCA ACAGAAACAA CAATTTCCAG ACCTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCGA CGGGGGAGGC CTGGCAGGAA ATCATGTTAG CCTGCCTCCC GGGGAAGCAA 
TGTGACCCCG ACTCGGATTA CAACCCCGGG GAGGAATACA CATGTGGGAG CAACTTCGCC 
ATCGTTTATT TCATCAGTTT TTATATGCTC TGCGCATTTC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGATTATCTC ACCCGGGACT GGTCTATTCT GGGGCCTCAC 
CATTTAGATG AATTCAAACG GATCTGGTCT GAATATGACC CCGAGGCAAA GGGCAGGATC 
AAGCACCTGG ACGTGGTCAC TCTGCTCCGC CGCATCCAGC CTCCCCTGGG CTTCGGGAAG 
TTGTGTCCAC ACCGGGTAGC CTGCAAGAGA CTAGTTGCTA TGAACATGCC ACTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACTCTG TTTGCTCTGG TTCGAACGGC TCTTAAAATC 
AAGACTGAAG GCAACCTGGA ACAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ACTACTTGAC CAAGTTGTTC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCCGCGA AGAACAGCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGC 
GATTTGCAAG ATGATGATCC AGAGGAAACA AAACGAGAAG ACGAAGAAGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT GCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCTTGTG 
TCTGAAAACG GGCATCATTC CTTCCACAAG CATGGCCGTG AGCCTCACAG AAGGTGCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCTG ACTCAGGAGA CGAGCAGTTC 
CCGACGATTT GCCGGGAGGA CCCGGAGATA CACGGCTACT TCAGGGACCC CCACTGCCCA 
GGGGAGCAGG AGTATTTCAG CAGCGAGGAG TACTATGAGG ATGACAGCTC GCCCACCGGG 
AGCAGGCAAA GCTACGGCTG CGGCTACTAT AACTGGTACC TGGGCAGCAG CTTGGACTTT 
GAGTGGCCTC GAGGCTACCA TCACCCCCAA AGCTTCTCAG AGGACGACGA CTCGCCCGTT 
GGCTACGATT CCCGGAGGTC TCCGAGGCGA CGCCTACTAC CTCCCACCCC TACATCCCCC 
CGCAGGTCTT CCTTCAACTT CGAGTGCCTG CGCCGGCAGA GCAGCCAGGA GGAGGTCCCG 
CCGTCGCCCG CCTTCCCGCC CCGCACGGCC CTGCCTCTGC ACCTGATGCA GCAGCAGATC 
ATGGCAGTCG CCGGCCTAGA TTCCAGTAAA GCCCAGAAGT ACTCGCCGAG CCACTCCACC 
CGGTCGTGGG CCACGCCCCC GGCCACGCCG TCGTATCGGG ACTGGACGCC GTGCTATACC 
CCGCTGATCC AGGTTGAGCG GCCAGAGGCC CCAGACCAGG TCAACGGCAG CCTCCCGTCC 
CTGCACCGCA GCTCGTGGTA CACGGACGAG CCCAGCGTCT CCTATCGGAC GTTCACACCA 
GCCAGCCTGA CCGTCCCCAG CAGCTTCCAC AACAAGAACA GCGACAAGCA GAGGAGCGCG 
GACAGCCTGG TGGAGGCAGT TCTGATATCC GAAGGCTTAG GACGGTACGC GAGGGACCCA 
AAGTTCGTGT CGGCGACAAA ACACGAAATC GCCGATGCCT GTGACTTAAC CATCGACGAG 
ATGGAGAGTG CGGCCAGCAA CCTGCTGAAC GGGAACGTGC ATCCGCGGGC CAATGGAGAC 
GTGGGCCCCG TTTCCCACCG GCAAGACTAC GAGCTCCAGG ACTTTGGGCC TGGCTACAGC 
GACGAGGAGC CGGACCCCGG GAGGGACGAG GAGGACCTGG CAGACGAGAT GATTTGCATC 
ACCACCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_005222720.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222777.1 Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X7, mRNA.

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CloneID	OBa31497
Clone ID Related Accession (Same CDS sequence)	XM_005222720.3 , XM_005222720.4
Accession Version	XM_005222720.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222720.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CCAACTGAAA GTGAAAGTGT CCCTGTCCCA 
ACCGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACGCTGCTG 
TGGACTTTTA TTAAGTCCTT CCAGGCGCTC CCGTATGTCG CCCTTCTTAT CGCCATGCTG 
TTCTTCATCT ACGCGGTCAT CGGCATGCAG ATGTTCGGGA AAGTGGCCAT GAGAGATAAC 
AACCAGATCA ACAGAAACAA CAATTTCCAG ACCTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCGA CGGGGGAGGC CTGGCAGGAA ATCATGTTAG CCTGCCTCCC GGGGAAGCAA 
TGTGACCCCG ACTCGGATTA CAACCCCGGG GAGGAATACA CATGTGGGAG CAACTTCGCC 
ATCGTTTATT TCATCAGTTT TTATATGCTC TGCGCATTTC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGATTATCTC ACCCGGGACT GGTCTATTCT GGGGCCTCAC 
CATTTAGATG AATTCAAACG GATCTGGTCT GAATATGACC CCGAGGCAAA GGGCAGGATC 
AAGCACCTGG ACGTGGTCAC TCTGCTCCGC CGCATCCAGC CTCCCCTGGG CTTCGGGAAG 
TTGTGTCCAC ACCGGGTAGC CTGCAAGAGA CTAGTTGCTA TGAACATGCC ACTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACTCTG TTTGCTCTGG TTCGAACGGC TCTTAAAATC 
AAGACTGAAG GCAACCTGGA ACAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ACTACTTGAC CAAGTTGTTC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCCGCGA AGAACAGCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGC 
GATTTGCAAG ATGATGATCC AGAGGAAACA AAACGAGAAG ACGAAGAAGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT GCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCTTGTG 
TCTGAAAACG GGCATCATTC CTTCCACAAG CATGGCCGTG AGCCTCACAG AAGGTGCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCTG ACTCAGGAGA CGAGCAGTTC 
CCGACGATTT GCCGGGAGGA CCCGGAGATA CACGGCTACT TCAGGGACCC CCACTGCCCA 
GGGGAGCAGG AGTATTTCAG CAGCGAGGAG TACTATGAGG ATGACAGCTC GCCCACCGGG 
AGCAGGCAAA GCTACGGCTG CGGCTACTAT AACTGGTACC TGGGCAGCAG CTTGGACTTT 
GAGTGGCCTC GAGGCTACCA TCACCCCCAA AGCTTCTCAG AGGACGACGA CTCGCCCGTT 
GGCTACGATT CCCGGAGGTC TCCGAGGCGA CGCCTACTAC CTCCCACCCC TACATCCCCC 
CGCAGGTCTT CCTTCAACTT CGAGTGCCTG CGCCGGCAGA GCAGCCAGGA GGAGGTCCCG 
CCGTCGCCCG CCTTCCCGCC CCGCACGGCC CTGCCTCTGC ACCTGATGCA GCAGCAGATC 
ATGGCAGTCG CCGGCCTAGA TTCCAGTAAA GCCCAGAAGT ACTCGCCGAG CCACTCCACC 
CGGTCGTGGG CCACGCCCCC GGCCACGCCG TCGTATCGGG ACTGGACGCC GTGCTATACC 
CCGCTGATCC AGGTTGAGCG GCCAGAGGCC CCAGACCAGG TCAACGGCAG CCTCCCGTCC 
CTGCACCGCA GCTCGTGGTA CACGGACGAG CCCAGCGTCT CCTATCGGAC GTTCACACCA 
GCCAGCCTGA CCGTCCCCAG CAGCTTCCAC AACAAGAACA GCGACAAGCA GAGGAGCGCG 
GACAGCCTGG TGGAGGCAGT TCTGATATCC GAAGGCTTAG GACGGTACGC GAGGGACCCA 
AAGTTCGTGT CGGCGACAAA ACACGAAATC GCCGATGCCT GTGACTTAAC CATCGACGAG 
ATGGAGAGTG CGGCCAGCAA CCTGCTGAAC GGGAACGTGC ATCCGCGGGC CAATGGAGAC 
GTGGGCCCCG TTTCCCACCG GCAAGACTAC GAGCTCCAGG ACTTTGGGCC TGGCTACAGC 
GACGAGGAGC CGGACCCCGG GAGGGACGAG GAGGACCTGG CAGACGAGAT GATTTGCATC 
ACCACCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222777.1
CDS	375..6866
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADPTESESVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_005222720.4
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222720.4

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CCAACTGAAA GTGAAAGTGT CCCTGTCCCA 
ACCGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACGCTGCTG 
TGGACTTTTA TTAAGTCCTT CCAGGCGCTC CCGTATGTCG CCCTTCTTAT CGCCATGCTG 
TTCTTCATCT ACGCGGTCAT CGGCATGCAG ATGTTCGGGA AAGTGGCCAT GAGAGATAAC 
AACCAGATCA ACAGAAACAA CAATTTCCAG ACCTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCGA CGGGGGAGGC CTGGCAGGAA ATCATGTTAG CCTGCCTCCC GGGGAAGCAA 
TGTGACCCCG ACTCGGATTA CAACCCCGGG GAGGAATACA CATGTGGGAG CAACTTCGCC 
ATCGTTTATT TCATCAGTTT TTATATGCTC TGCGCATTTC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGATTATCTC ACCCGGGACT GGTCTATTCT GGGGCCTCAC 
CATTTAGATG AATTCAAACG GATCTGGTCT GAATATGACC CCGAGGCAAA GGGCAGGATC 
AAGCACCTGG ACGTGGTCAC TCTGCTCCGC CGCATCCAGC CTCCCCTGGG CTTCGGGAAG 
TTGTGTCCAC ACCGGGTAGC CTGCAAGAGA CTAGTTGCTA TGAACATGCC ACTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACTCTG TTTGCTCTGG TTCGAACGGC TCTTAAAATC 
AAGACTGAAG GCAACCTGGA ACAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ACTACTTGAC CAAGTTGTTC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCCGCGA AGAACAGCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGC 
GATTTGCAAG ATGATGATCC AGAGGAAACA AAACGAGAAG ACGAAGAAGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT GCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCTTGTG 
TCTGAAAACG GGCATCATTC CTTCCACAAG CATGGCCGTG AGCCTCACAG AAGGTGCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCTG ACTCAGGAGA CGAGCAGTTC 
CCGACGATTT GCCGGGAGGA CCCGGAGATA CACGGCTACT TCAGGGACCC CCACTGCCCA 
GGGGAGCAGG AGTATTTCAG CAGCGAGGAG TACTATGAGG ATGACAGCTC GCCCACCGGG 
AGCAGGCAAA GCTACGGCTG CGGCTACTAT AACTGGTACC TGGGCAGCAG CTTGGACTTT 
GAGTGGCCTC GAGGCTACCA TCACCCCCAA AGCTTCTCAG AGGACGACGA CTCGCCCGTT 
GGCTACGATT CCCGGAGGTC TCCGAGGCGA CGCCTACTAC CTCCCACCCC TACATCCCCC 
CGCAGGTCTT CCTTCAACTT CGAGTGCCTG CGCCGGCAGA GCAGCCAGGA GGAGGTCCCG 
CCGTCGCCCG CCTTCCCGCC CCGCACGGCC CTGCCTCTGC ACCTGATGCA GCAGCAGATC 
ATGGCAGTCG CCGGCCTAGA TTCCAGTAAA GCCCAGAAGT ACTCGCCGAG CCACTCCACC 
CGGTCGTGGG CCACGCCCCC GGCCACGCCG TCGTATCGGG ACTGGACGCC GTGCTATACC 
CCGCTGATCC AGGTTGAGCG GCCAGAGGCC CCAGACCAGG TCAACGGCAG CCTCCCGTCC 
CTGCACCGCA GCTCGTGGTA CACGGACGAG CCCAGCGTCT CCTATCGGAC GTTCACACCA 
GCCAGCCTGA CCGTCCCCAG CAGCTTCCAC AACAAGAACA GCGACAAGCA GAGGAGCGCG 
GACAGCCTGG TGGAGGCAGT TCTGATATCC GAAGGCTTAG GACGGTACGC GAGGGACCCA 
AAGTTCGTGT CGGCGACAAA ACACGAAATC GCCGATGCCT GTGACTTAAC CATCGACGAG 
ATGGAGAGTG CGGCCAGCAA CCTGCTGAAC GGGAACGTGC ATCCGCGGGC CAATGGAGAC 
GTGGGCCCCG TTTCCCACCG GCAAGACTAC GAGCTCCAGG ACTTTGGGCC TGGCTACAGC 
GACGAGGAGC CGGACCCCGG GAGGGACGAG GAGGACCTGG CAGACGAGAT GATTTGCATC 
ACCACCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_005222721.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222778.1 Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X4, mRNA.

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CloneID	OBa31498
Clone ID Related Accession (Same CDS sequence)	XM_005222721.3
Accession Version	XM_005222721.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222721.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222778.1
CDS	1..6447
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_005222721.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222721.3

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_005222722.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222779.1 Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X8, mRNA.

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CloneID	OBa31499
Clone ID Related Accession (Same CDS sequence)	XM_005222722.4 , XM_005222722.3
Accession Version	XM_005222722.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222722.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGGTCTGAC TCAGGAGACG AGCAGTTCCC GACGATTTGC 
CGGGAGGACC CGGAGATACA CGGCTACTTC AGGGACCCCC ACTGCCCAGG GGAGCAGGAG 
TATTTCAGCA GCGAGGAGTA CTATGAGGAT GACAGCTCGC CCACCGGGAG CAGGCAAAGC 
TACGGCTGCG GCTACTATAA CTGGTACCTG GGCAGCAGCT TGGACTTTGA GTGGCCTCGA 
GGCTACCATC ACCCCCAAAG CTTCTCAGAG GACGACGACT CGCCCGTTGG CTACGATTCC 
CGGAGGTCTC CGAGGCGACG CCTACTACCT CCCACCCCTA CATCCCCCCG CAGGTCTTCC 
TTCAACTTCG AGTGCCTGCG CCGGCAGAGC AGCCAGGAGG AGGTCCCGCC GTCGCCCGCC 
TTCCCGCCCC GCACGGCCCT GCCTCTGCAC CTGATGCAGC AGCAGATCAT GGCAGTCGCC 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCGCCGAGCC ACTCCACCCG GTCGTGGGCC 
ACGCCCCCGG CCACGCCGTC GTATCGGGAC TGGACGCCGT GCTATACCCC GCTGATCCAG 
GTTGAGCGGC CAGAGGCCCC AGACCAGGTC AACGGCAGCC TCCCGTCCCT GCACCGCAGC 
TCGTGGTACA CGGACGAGCC CAGCGTCTCC TATCGGACGT TCACACCAGC CAGCCTGACC 
GTCCCCAGCA GCTTCCACAA CAAGAACAGC GACAAGCAGA GGAGCGCGGA CAGCCTGGTG 
GAGGCAGTTC TGATATCCGA AGGCTTAGGA CGGTACGCGA GGGACCCAAA GTTCGTGTCG 
GCGACAAAAC ACGAAATCGC CGATGCCTGT GACTTAACCA TCGACGAGAT GGAGAGTGCG 
GCCAGCAACC TGCTGAACGG GAACGTGCAT CCGCGGGCCA ATGGAGACGT GGGCCCCGTT 
TCCCACCGGC AAGACTACGA GCTCCAGGAC TTTGGGCCTG GCTACAGCGA CGAGGAGCCG 
GACCCCGGGA GGGACGAGGA GGACCTGGCA GACGAGATGA TTTGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222779.1
CDS	374..6793
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_005222722.4
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222722.4

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGGTCTGAC TCAGGAGACG AGCAGTTCCC GACGATTTGC 
CGGGAGGACC CGGAGATACA CGGCTACTTC AGGGACCCCC ACTGCCCAGG GGAGCAGGAG 
TATTTCAGCA GCGAGGAGTA CTATGAGGAT GACAGCTCGC CCACCGGGAG CAGGCAAAGC 
TACGGCTGCG GCTACTATAA CTGGTACCTG GGCAGCAGCT TGGACTTTGA GTGGCCTCGA 
GGCTACCATC ACCCCCAAAG CTTCTCAGAG GACGACGACT CGCCCGTTGG CTACGATTCC 
CGGAGGTCTC CGAGGCGACG CCTACTACCT CCCACCCCTA CATCCCCCCG CAGGTCTTCC 
TTCAACTTCG AGTGCCTGCG CCGGCAGAGC AGCCAGGAGG AGGTCCCGCC GTCGCCCGCC 
TTCCCGCCCC GCACGGCCCT GCCTCTGCAC CTGATGCAGC AGCAGATCAT GGCAGTCGCC 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCGCCGAGCC ACTCCACCCG GTCGTGGGCC 
ACGCCCCCGG CCACGCCGTC GTATCGGGAC TGGACGCCGT GCTATACCCC GCTGATCCAG 
GTTGAGCGGC CAGAGGCCCC AGACCAGGTC AACGGCAGCC TCCCGTCCCT GCACCGCAGC 
TCGTGGTACA CGGACGAGCC CAGCGTCTCC TATCGGACGT TCACACCAGC CAGCCTGACC 
GTCCCCAGCA GCTTCCACAA CAAGAACAGC GACAAGCAGA GGAGCGCGGA CAGCCTGGTG 
GAGGCAGTTC TGATATCCGA AGGCTTAGGA CGGTACGCGA GGGACCCAAA GTTCGTGTCG 
GCGACAAAAC ACGAAATCGC CGATGCCTGT GACTTAACCA TCGACGAGAT GGAGAGTGCG 
GCCAGCAACC TGCTGAACGG GAACGTGCAT CCGCGGGCCA ATGGAGACGT GGGCCCCGTT 
TCCCACCGGC AAGACTACGA GCTCCAGGAC TTTGGGCCTG GCTACAGCGA CGAGGAGCCG 
GACCCCGGGA GGGACGAGGA GGACCTGGCA GACGAGATGA TTTGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_005222722.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222779.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X14, mRNA.

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CloneID	OBa31499
Clone ID Related Accession (Same CDS sequence)	XM_005222722.4 , XM_005222722.3
Accession Version	XM_005222722.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222722.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGGTCTGAC TCAGGAGACG AGCAGTTCCC GACGATTTGC 
CGGGAGGACC CGGAGATACA CGGCTACTTC AGGGACCCCC ACTGCCCAGG GGAGCAGGAG 
TATTTCAGCA GCGAGGAGTA CTATGAGGAT GACAGCTCGC CCACCGGGAG CAGGCAAAGC 
TACGGCTGCG GCTACTATAA CTGGTACCTG GGCAGCAGCT TGGACTTTGA GTGGCCTCGA 
GGCTACCATC ACCCCCAAAG CTTCTCAGAG GACGACGACT CGCCCGTTGG CTACGATTCC 
CGGAGGTCTC CGAGGCGACG CCTACTACCT CCCACCCCTA CATCCCCCCG CAGGTCTTCC 
TTCAACTTCG AGTGCCTGCG CCGGCAGAGC AGCCAGGAGG AGGTCCCGCC GTCGCCCGCC 
TTCCCGCCCC GCACGGCCCT GCCTCTGCAC CTGATGCAGC AGCAGATCAT GGCAGTCGCC 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCGCCGAGCC ACTCCACCCG GTCGTGGGCC 
ACGCCCCCGG CCACGCCGTC GTATCGGGAC TGGACGCCGT GCTATACCCC GCTGATCCAG 
GTTGAGCGGC CAGAGGCCCC AGACCAGGTC AACGGCAGCC TCCCGTCCCT GCACCGCAGC 
TCGTGGTACA CGGACGAGCC CAGCGTCTCC TATCGGACGT TCACACCAGC CAGCCTGACC 
GTCCCCAGCA GCTTCCACAA CAAGAACAGC GACAAGCAGA GGAGCGCGGA CAGCCTGGTG 
GAGGCAGTTC TGATATCCGA AGGCTTAGGA CGGTACGCGA GGGACCCAAA GTTCGTGTCG 
GCGACAAAAC ACGAAATCGC CGATGCCTGT GACTTAACCA TCGACGAGAT GGAGAGTGCG 
GCCAGCAACC TGCTGAACGG GAACGTGCAT CCGCGGGCCA ATGGAGACGT GGGCCCCGTT 
TCCCACCGGC AAGACTACGA GCTCCAGGAC TTTGGGCCTG GCTACAGCGA CGAGGAGCCG 
GACCCCGGGA GGGACGAGGA GGACCTGGCA GACGAGATGA TTTGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222779.1
CDS	1..6420
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_005222722.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222722.3

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGGTCTGAC TCAGGAGACG AGCAGTTCCC GACGATTTGC 
CGGGAGGACC CGGAGATACA CGGCTACTTC AGGGACCCCC ACTGCCCAGG GGAGCAGGAG 
TATTTCAGCA GCGAGGAGTA CTATGAGGAT GACAGCTCGC CCACCGGGAG CAGGCAAAGC 
TACGGCTGCG GCTACTATAA CTGGTACCTG GGCAGCAGCT TGGACTTTGA GTGGCCTCGA 
GGCTACCATC ACCCCCAAAG CTTCTCAGAG GACGACGACT CGCCCGTTGG CTACGATTCC 
CGGAGGTCTC CGAGGCGACG CCTACTACCT CCCACCCCTA CATCCCCCCG CAGGTCTTCC 
TTCAACTTCG AGTGCCTGCG CCGGCAGAGC AGCCAGGAGG AGGTCCCGCC GTCGCCCGCC 
TTCCCGCCCC GCACGGCCCT GCCTCTGCAC CTGATGCAGC AGCAGATCAT GGCAGTCGCC 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCGCCGAGCC ACTCCACCCG GTCGTGGGCC 
ACGCCCCCGG CCACGCCGTC GTATCGGGAC TGGACGCCGT GCTATACCCC GCTGATCCAG 
GTTGAGCGGC CAGAGGCCCC AGACCAGGTC AACGGCAGCC TCCCGTCCCT GCACCGCAGC 
TCGTGGTACA CGGACGAGCC CAGCGTCTCC TATCGGACGT TCACACCAGC CAGCCTGACC 
GTCCCCAGCA GCTTCCACAA CAAGAACAGC GACAAGCAGA GGAGCGCGGA CAGCCTGGTG 
GAGGCAGTTC TGATATCCGA AGGCTTAGGA CGGTACGCGA GGGACCCAAA GTTCGTGTCG 
GCGACAAAAC ACGAAATCGC CGATGCCTGT GACTTAACCA TCGACGAGAT GGAGAGTGCG 
GCCAGCAACC TGCTGAACGG GAACGTGCAT CCGCGGGCCA ATGGAGACGT GGGCCCCGTT 
TCCCACCGGC AAGACTACGA GCTCCAGGAC TTTGGGCCTG GCTACAGCGA CGAGGAGCCG 
GACCCCGGGA GGGACGAGGA GGACCTGGCA GACGAGATGA TTTGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817670.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815972.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X3, mRNA.

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CloneID	OBa45767
Clone ID Related Accession (Same CDS sequence)	XM_010817670.1
Accession Version	XM_010817670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGGTGAAGA GGAAGAGGAG GAGGAAGAGG ATGAACCTGA GGTTCCTGCT 
GGCCCCCGTC CTCGCAGAAT CTCTGAGTTG AACATGAAAG AGAAAATCGC CCCCATCCCG 
GAAGGGAGTG CTTTCTTCAT TCTCAGCAAG ACCAACCCGA TCCGCGTGGG CTGCCACAAG 
CTCATCAACC ACCACATCTT CACCAACCTC ATCCTTGTCT TCATCATGCT GAGCAGCGCC 
GCCCTCGCCG CTGAGGACCC CATCCGCAGC CACTCCTTCC GGAACACTAT ACTGGGTTAC 
TTTGACTATG CTTTCACAGC CATCTTTACT GTTGAAATCC TGTTGAAGAT GATGACCTTT 
GGCGCTTTCC TCCACAAAGG CGCTTTCTGC AGGAACTACT TCAACTTACT GGATATGCTG 
GTTGTTGGGG TGTCTTTGGT GTCATTTGGC ATCCAATCCA GTGCCATCTC TGTTGTGAAG 
ATTCTGAGGG TCCTAAGGGT CCTGAGGCCT CTCAGGGCCA TCAACAGAGC GAAAGGACTT 
AAGCATGTGG TCCAGTGTGT CTTCGTGGCC ATCCGGACGA TTGGCAACAT CATGATCGTC 
ACCACGCTCC TCCAGTTCAT GTTCGCCTGC ATCGGGGTCC AGCTCTTCAA GGGAAAGTTC 
TATCGCTGCA CAGACGAAGC CAAAAGTAAC CCCGAAGAGT GCAGGGGGCT CTTCATCCTC 
TACAAGGATG GGGATGTTGA TAGTCCTGTG GTCCGTGAGA GGATCTGGCA AAACAGTGAT 
TTCAACTTTG ACAACGTCCT CTCGGCTATG ATGGCTCTGT TCACAGTCTC CACGTTCGAG 
GGCTGGCCCG CGTTGCTGTA CAAAGCCATC GATTCGAACG GAGAGAATGT GGGCCCCATC 
TACAACTACC GTGTGGAGAT CTCCATCTTC TTCATCATCT ACATCATCAT TGTCGCATTC 
TTCATGATGA ACATCTTCGT GGGCTTCGTC ATCGTCACAT TTCAGGAGCA GGGAGAGAAA 
GAGTATAAGA ACTGTGAGCT GGACAAAAAT CAGCGTCAGT GTGTTGAATA CGCCTTGAAG 
GCACGTCCGC TGCGGAGATA CATCCCCAAA AACCCCTACC AGTACAAGTT CTGGTACGTG 
GTGAACTCCT CGCCTTTCGA ATACATGATG TTTGTCCTCA TCATGCTCAA CACACTCTGC 
TTGGCCATGC AGCACTACGA GCAGTCCAAG ATGTTTAATG ACGCCATGGA CATCCTGAAC 
ATGGTCTTCA CTGGGGTGTT CACTGTCGAG ATGGTTTTGA AAGTCATTGC ATTTAAGCCT 
AAGGGGTACT TTAGTGACGC CTGGAACACC TTTGACTCCC TCATCGTAAT CGGCAGCATT 
GTAGACGTGG CCCTCAGCGA AGCCGACCAC TATTTCACTG ATGCATGGAA CACTTTTGAT 
GCCTTAATTG TTGTTGGTAG CGTCGTTGAT ATTGCTATAA CTGAAGTGAA TCCAACTGAA 
AGTGAAAGTG TCCCTGTCCC AACCGCTACA CCTGGGAACT CTGAAGAGAG CAATAGAATC 
TCCATCACCT TTTTCCGTCT TTTCCGAGTG ATGCGATTGG TGAAGCTTCT CAGCAGGGGG 
GAAGGCATCC GGACGCTGCT GTGGACTTTT ATTAAGTCCT TCCAGGCGCT CCCGTATGTC 
GCCCTTCTTA TCGCCATGCT GTTCTTCATC TACGCGGTCA TCGGCATGCA GATGTTCGGG 
AAAGTGGCCA TGAGAGATAA CAACCAGATC AACAGAAACA ACAATTTCCA GACCTTTCCC 
CAGGCGGTGC TGCTGCTCTT CAGGTGTGCG ACGGGGGAGG CCTGGCAGGA AATCATGTTA 
GCCTGCCTCC CGGGGAAGCA ATGTGACCCC GACTCGGATT ACAACCCCGG GGAGGAATAC 
ACATGTGGGA GCAACTTCGC CATCGTTTAT TTCATCAGTT TTTATATGCT CTGCGCATTT 
CTGATCATCA ACCTCTTCGT GGCTGTCATC ATGGACAATT TTGATTATCT CACCCGGGAC 
TGGTCTATTC TGGGGCCTCA CCATTTAGAT GAATTCAAAC GGATCTGGTC TGAATATGAC 
CCCGAGGCAA AGGGCAGGAT CAAGCACCTG GACGTGGTCA CTCTGCTCCG CCGCATCCAG 
CCTCCCCTGG GCTTCGGGAA GTTGTGTCCA CACCGGGTAG CCTGCAAGAG ACTAGTTGCT 
ATGAACATGC CACTCAACAG TGATGGGACA GTCATGTTTA ATGCAACTCT GTTTGCTCTG 
GTTCGAACGG CTCTTAAAAT CAAGACTGAA GGCAACCTGG AACAAGCTAA TGAAGAACTC 
CGAGCTGTGA TCAAGAAAAT TTGGAAGAAA ACCAGCATGA AACTACTTGA CCAAGTTGTT 
CCTCCAGCTG GTGATGATGA GGTAACCGTG GGGAAGTTCT ATGCCACTTT CCTGATACAG 
GACTACTTTA GGAAATTCAA GAAACGGAAA GAACAAGGAC TGGTGGGAAA ATACCCCGCG 
AAGAACAGCA CAATTGCCCT ACAGGCGGGA TTAAGGACAC TGCATGACAT TGGGCCAGAA 
ATCCGGCGTG CTATATCATG CGATTTGCAA GATGATGATC CAGAGGAAAC AAAACGAGAA 
GACGAAGAAG ATGTGTTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATTCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGTGT GTCATAACCA TCATAACCAT AATTCCATAG GAAAGCAAGT TCCCAGCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TGCAAAGCTG CCCCTGGAAA GCGGCCCAGC 
ATTGGGAACC TTGAGCTTGT GTCTGAAAAC GGGCATCATT CCTTCCACAA GCATGGCCGT 
GAGCCTCACA GAAGGTGCAG TATTAAAAGA ACCCGCTATT ATGAAACTTA CATTAGGTCT 
GACTCAGGAG ACGAGCAGTT CCCGACGATT TGCCGGGAGG ACCCGGAGAT ACACGGCTAC 
TTCAGGGACC CCCACTGCCC AGGGGAGCAG GAGTATTTCA GCAGCGAGGA GTACTATGAG 
GATGACAGCT CGCCCACCGG GAGCAGGCAA AGCTACGGCT GCGGCTACTA TAACTGGTAC 
CTGGGCAGCA GCTTGGACTT TGAGTGGCCT CGAGGCTACC ATCACCCCCA AAGCTTCTCA 
GAGGACGACG ACTCGCCCGT TGGCTACGAT TCCCGGAGGT CTCCGAGGCG ACGCCTACTA 
CCTCCCACCC CTACATCCCC CCGCAGGTCT TCCTTCAACT TCGAGTGCCT GCGCCGGCAG 
AGCAGCCAGG AGGAGGTCCC GCCGTCGCCC GCCTTCCCGC CCCGCACGGC CCTGCCTCTG 
CACCTGATGC AGCAGCAGAT CATGGCAGTC GCCGGCCTAG ATTCCAGTAA AGCCCAGAAG 
TACTCGCCGA GCCACTCCAC CCGGTCGTGG GCCACGCCCC CGGCCACGCC GTCGTATCGG 
GACTGGACGC CGTGCTATAC CCCGCTGATC CAGGTTGAGC GGCCAGAGGC CCCAGACCAG 
GTCAACGGCA GCCTCCCGTC CCTGCACCGC AGCTCGTGGT ACACGGACGA GCCCAGCGTC 
TCCTATCGGA CGTTCACACC AGCCAGCCTG ACCGTCCCCA GCAGCTTCCA CAACAAGAAC 
AGCGACAAGC AGAGGAGCGC GGACAGCCTG GTGGAGGCAG TTCTGATATC CGAAGGCTTA 
GGACGGTACG CGAGGGACCC AAAGTTCGTG TCGGCGACAA AACACGAAAT CGCCGATGCC 
TGTGACTTAA CCATCGACGA GATGGAGAGT GCGGCCAGCA ACCTGCTGAA CGGGAACGTG 
CATCCGCGGG CCAATGGAGA CGTGGGCCCC GTTTCCCACC GGCAAGACTA CGAGCTCCAG 
GACTTTGGGC CTGGCTACAG CGACGAGGAG CCGGACCCCG GGAGGGACGA GGAGGACCTG 
GCAGACGAGA TGATTTGCAT CACCACCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815972.1
CDS	259..6891
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESESVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817670.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817670.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGGTGAAGA GGAAGAGGAG GAGGAAGAGG ATGAACCTGA GGTTCCTGCT 
GGCCCCCGTC CTCGCAGAAT CTCTGAGTTG AACATGAAAG AGAAAATCGC CCCCATCCCG 
GAAGGGAGTG CTTTCTTCAT TCTCAGCAAG ACCAACCCGA TCCGCGTGGG CTGCCACAAG 
CTCATCAACC ACCACATCTT CACCAACCTC ATCCTTGTCT TCATCATGCT GAGCAGCGCC 
GCCCTCGCCG CTGAGGACCC CATCCGCAGC CACTCCTTCC GGAACACTAT ACTGGGTTAC 
TTTGACTATG CTTTCACAGC CATCTTTACT GTTGAAATCC TGTTGAAGAT GATGACCTTT 
GGCGCTTTCC TCCACAAAGG CGCTTTCTGC AGGAACTACT TCAACTTACT GGATATGCTG 
GTTGTTGGGG TGTCTTTGGT GTCATTTGGC ATCCAATCCA GTGCCATCTC TGTTGTGAAG 
ATTCTGAGGG TCCTAAGGGT CCTGAGGCCT CTCAGGGCCA TCAACAGAGC GAAAGGACTT 
AAGCATGTGG TCCAGTGTGT CTTCGTGGCC ATCCGGACGA TTGGCAACAT CATGATCGTC 
ACCACGCTCC TCCAGTTCAT GTTCGCCTGC ATCGGGGTCC AGCTCTTCAA GGGAAAGTTC 
TATCGCTGCA CAGACGAAGC CAAAAGTAAC CCCGAAGAGT GCAGGGGGCT CTTCATCCTC 
TACAAGGATG GGGATGTTGA TAGTCCTGTG GTCCGTGAGA GGATCTGGCA AAACAGTGAT 
TTCAACTTTG ACAACGTCCT CTCGGCTATG ATGGCTCTGT TCACAGTCTC CACGTTCGAG 
GGCTGGCCCG CGTTGCTGTA CAAAGCCATC GATTCGAACG GAGAGAATGT GGGCCCCATC 
TACAACTACC GTGTGGAGAT CTCCATCTTC TTCATCATCT ACATCATCAT TGTCGCATTC 
TTCATGATGA ACATCTTCGT GGGCTTCGTC ATCGTCACAT TTCAGGAGCA GGGAGAGAAA 
GAGTATAAGA ACTGTGAGCT GGACAAAAAT CAGCGTCAGT GTGTTGAATA CGCCTTGAAG 
GCACGTCCGC TGCGGAGATA CATCCCCAAA AACCCCTACC AGTACAAGTT CTGGTACGTG 
GTGAACTCCT CGCCTTTCGA ATACATGATG TTTGTCCTCA TCATGCTCAA CACACTCTGC 
TTGGCCATGC AGCACTACGA GCAGTCCAAG ATGTTTAATG ACGCCATGGA CATCCTGAAC 
ATGGTCTTCA CTGGGGTGTT CACTGTCGAG ATGGTTTTGA AAGTCATTGC ATTTAAGCCT 
AAGGGGTACT TTAGTGACGC CTGGAACACC TTTGACTCCC TCATCGTAAT CGGCAGCATT 
GTAGACGTGG CCCTCAGCGA AGCCGACCAC TATTTCACTG ATGCATGGAA CACTTTTGAT 
GCCTTAATTG TTGTTGGTAG CGTCGTTGAT ATTGCTATAA CTGAAGTGAA TCCAACTGAA 
AGTGAAAGTG TCCCTGTCCC AACCGCTACA CCTGGGAACT CTGAAGAGAG CAATAGAATC 
TCCATCACCT TTTTCCGTCT TTTCCGAGTG ATGCGATTGG TGAAGCTTCT CAGCAGGGGG 
GAAGGCATCC GGACGCTGCT GTGGACTTTT ATTAAGTCCT TCCAGGCGCT CCCGTATGTC 
GCCCTTCTTA TCGCCATGCT GTTCTTCATC TACGCGGTCA TCGGCATGCA GATGTTCGGG 
AAAGTGGCCA TGAGAGATAA CAACCAGATC AACAGAAACA ACAATTTCCA GACCTTTCCC 
CAGGCGGTGC TGCTGCTCTT CAGGTGTGCG ACGGGGGAGG CCTGGCAGGA AATCATGTTA 
GCCTGCCTCC CGGGGAAGCA ATGTGACCCC GACTCGGATT ACAACCCCGG GGAGGAATAC 
ACATGTGGGA GCAACTTCGC CATCGTTTAT TTCATCAGTT TTTATATGCT CTGCGCATTT 
CTGATCATCA ACCTCTTCGT GGCTGTCATC ATGGACAATT TTGATTATCT CACCCGGGAC 
TGGTCTATTC TGGGGCCTCA CCATTTAGAT GAATTCAAAC GGATCTGGTC TGAATATGAC 
CCCGAGGCAA AGGGCAGGAT CAAGCACCTG GACGTGGTCA CTCTGCTCCG CCGCATCCAG 
CCTCCCCTGG GCTTCGGGAA GTTGTGTCCA CACCGGGTAG CCTGCAAGAG ACTAGTTGCT 
ATGAACATGC CACTCAACAG TGATGGGACA GTCATGTTTA ATGCAACTCT GTTTGCTCTG 
GTTCGAACGG CTCTTAAAAT CAAGACTGAA GGCAACCTGG AACAAGCTAA TGAAGAACTC 
CGAGCTGTGA TCAAGAAAAT TTGGAAGAAA ACCAGCATGA AACTACTTGA CCAAGTTGTT 
CCTCCAGCTG GTGATGATGA GGTAACCGTG GGGAAGTTCT ATGCCACTTT CCTGATACAG 
GACTACTTTA GGAAATTCAA GAAACGGAAA GAACAAGGAC TGGTGGGAAA ATACCCCGCG 
AAGAACAGCA CAATTGCCCT ACAGGCGGGA TTAAGGACAC TGCATGACAT TGGGCCAGAA 
ATCCGGCGTG CTATATCATG CGATTTGCAA GATGATGATC CAGAGGAAAC AAAACGAGAA 
GACGAAGAAG ATGTGTTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATTCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGTGT GTCATAACCA TCATAACCAT AATTCCATAG GAAAGCAAGT TCCCAGCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TGCAAAGCTG CCCCTGGAAA GCGGCCCAGC 
ATTGGGAACC TTGAGCTTGT GTCTGAAAAC GGGCATCATT CCTTCCACAA GCATGGCCGT 
GAGCCTCACA GAAGGTGCAG TATTAAAAGA ACCCGCTATT ATGAAACTTA CATTAGGTCT 
GACTCAGGAG ACGAGCAGTT CCCGACGATT TGCCGGGAGG ACCCGGAGAT ACACGGCTAC 
TTCAGGGACC CCCACTGCCC AGGGGAGCAG GAGTATTTCA GCAGCGAGGA GTACTATGAG 
GATGACAGCT CGCCCACCGG GAGCAGGCAA AGCTACGGCT GCGGCTACTA TAACTGGTAC 
CTGGGCAGCA GCTTGGACTT TGAGTGGCCT CGAGGCTACC ATCACCCCCA AAGCTTCTCA 
GAGGACGACG ACTCGCCCGT TGGCTACGAT TCCCGGAGGT CTCCGAGGCG ACGCCTACTA 
CCTCCCACCC CTACATCCCC CCGCAGGTCT TCCTTCAACT TCGAGTGCCT GCGCCGGCAG 
AGCAGCCAGG AGGAGGTCCC GCCGTCGCCC GCCTTCCCGC CCCGCACGGC CCTGCCTCTG 
CACCTGATGC AGCAGCAGAT CATGGCAGTC GCCGGCCTAG ATTCCAGTAA AGCCCAGAAG 
TACTCGCCGA GCCACTCCAC CCGGTCGTGG GCCACGCCCC CGGCCACGCC GTCGTATCGG 
GACTGGACGC CGTGCTATAC CCCGCTGATC CAGGTTGAGC GGCCAGAGGC CCCAGACCAG 
GTCAACGGCA GCCTCCCGTC CCTGCACCGC AGCTCGTGGT ACACGGACGA GCCCAGCGTC 
TCCTATCGGA CGTTCACACC AGCCAGCCTG ACCGTCCCCA GCAGCTTCCA CAACAAGAAC 
AGCGACAAGC AGAGGAGCGC GGACAGCCTG GTGGAGGCAG TTCTGATATC CGAAGGCTTA 
GGACGGTACG CGAGGGACCC AAAGTTCGTG TCGGCGACAA AACACGAAAT CGCCGATGCC 
TGTGACTTAA CCATCGACGA GATGGAGAGT GCGGCCAGCA ACCTGCTGAA CGGGAACGTG 
CATCCGCGGG CCAATGGAGA CGTGGGCCCC GTTTCCCACC GGCAAGACTA CGAGCTCCAG 
GACTTTGGGC CTGGCTACAG CGACGAGGAG CCGGACCCCG GGAGGGACGA GGAGGACCTG 
GCAGACGAGA TGATTTGCAT CACCACCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817671.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815973.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X4, mRNA.

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CloneID	OBa45768
Clone ID Related Accession (Same CDS sequence)	XM_010817671.1
Accession Version	XM_010817671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA TTCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAATTCC ACCTGCAAGT GATACTGAGA AACCGCTGTT TCCTCCAGCA 
GGAAATTCGG TGTGTCATAA CCATCATAAC CATAATTCCA TAGGAAAGCA AGTTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTGCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCT TGTGTCTGAA AACGGGCATC ATTCCTTCCA CAAGCATGGC 
CGTGAGCCTC ACAGAAGGTG CAGTATTAAA AGGTCTGACT CAGGAGACGA GCAGTTCCCG 
ACGATTTGCC GGGAGGACCC GGAGATACAC GGCTACTTCA GGGACCCCCA CTGCCCAGGG 
GAGCAGGAGT ATTTCAGCAG CGAGGAGTAC TATGAGGATG ACAGCTCGCC CACCGGGAGC 
AGGCAAAGCT ACGGCTGCGG CTACTATAAC TGGTACCTGG GCAGCAGCTT GGACTTTGAG 
TGGCCTCGAG GCTACCATCA CCCCCAAAGC TTCTCAGAGG ACGACGACTC GCCCGTTGGC 
TACGATTCCC GGAGGTCTCC GAGGCGACGC CTACTACCTC CCACCCCTAC ATCCCCCCGC 
AGGTCTTCCT TCAACTTCGA GTGCCTGCGC CGGCAGAGCA GCCAGGAGGA GGTCCCGCCG 
TCGCCCGCCT TCCCGCCCCG CACGGCCCTG CCTCTGCACC TGATGCAGCA GCAGATCATG 
GCAGTCGCCG GCCTAGATTC CAGTAAAGCC CAGAAGTACT CGCCGAGCCA CTCCACCCGG 
TCGTGGGCCA CGCCCCCGGC CACGCCGTCG TATCGGGACT GGACGCCGTG CTATACCCCG 
CTGATCCAGG TTGAGCGGCC AGAGGCCCCA GACCAGGTCA ACGGCAGCCT CCCGTCCCTG 
CACCGCAGCT CGTGGTACAC GGACGAGCCC AGCGTCTCCT ATCGGACGTT CACACCAGCC 
AGCCTGACCG TCCCCAGCAG CTTCCACAAC AAGAACAGCG ACAAGCAGAG GAGCGCGGAC 
AGCCTGGTGG AGGCAGTTCT GATATCCGAA GGCTTAGGAC GGTACGCGAG GGACCCAAAG 
TTCGTGTCGG CGACAAAACA CGAAATCGCC GATGCCTGTG ACTTAACCAT CGACGAGATG 
GAGAGTGCGG CCAGCAACCT GCTGAACGGG AACGTGCATC CGCGGGCCAA TGGAGACGTG 
GGCCCCGTTT CCCACCGGCA AGACTACGAG CTCCAGGACT TTGGGCCTGG CTACAGCGAC 
GAGGAGCCGG ACCCCGGGAG GGACGAGGAG GACCTGGCAG ACGAGATGAT TTGCATCACC 
ACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815973.1
CDS	259..6867
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESESVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817671.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817671.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA TTCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAATTCC ACCTGCAAGT GATACTGAGA AACCGCTGTT TCCTCCAGCA 
GGAAATTCGG TGTGTCATAA CCATCATAAC CATAATTCCA TAGGAAAGCA AGTTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTGCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCT TGTGTCTGAA AACGGGCATC ATTCCTTCCA CAAGCATGGC 
CGTGAGCCTC ACAGAAGGTG CAGTATTAAA AGGTCTGACT CAGGAGACGA GCAGTTCCCG 
ACGATTTGCC GGGAGGACCC GGAGATACAC GGCTACTTCA GGGACCCCCA CTGCCCAGGG 
GAGCAGGAGT ATTTCAGCAG CGAGGAGTAC TATGAGGATG ACAGCTCGCC CACCGGGAGC 
AGGCAAAGCT ACGGCTGCGG CTACTATAAC TGGTACCTGG GCAGCAGCTT GGACTTTGAG 
TGGCCTCGAG GCTACCATCA CCCCCAAAGC TTCTCAGAGG ACGACGACTC GCCCGTTGGC 
TACGATTCCC GGAGGTCTCC GAGGCGACGC CTACTACCTC CCACCCCTAC ATCCCCCCGC 
AGGTCTTCCT TCAACTTCGA GTGCCTGCGC CGGCAGAGCA GCCAGGAGGA GGTCCCGCCG 
TCGCCCGCCT TCCCGCCCCG CACGGCCCTG CCTCTGCACC TGATGCAGCA GCAGATCATG 
GCAGTCGCCG GCCTAGATTC CAGTAAAGCC CAGAAGTACT CGCCGAGCCA CTCCACCCGG 
TCGTGGGCCA CGCCCCCGGC CACGCCGTCG TATCGGGACT GGACGCCGTG CTATACCCCG 
CTGATCCAGG TTGAGCGGCC AGAGGCCCCA GACCAGGTCA ACGGCAGCCT CCCGTCCCTG 
CACCGCAGCT CGTGGTACAC GGACGAGCCC AGCGTCTCCT ATCGGACGTT CACACCAGCC 
AGCCTGACCG TCCCCAGCAG CTTCCACAAC AAGAACAGCG ACAAGCAGAG GAGCGCGGAC 
AGCCTGGTGG AGGCAGTTCT GATATCCGAA GGCTTAGGAC GGTACGCGAG GGACCCAAAG 
TTCGTGTCGG CGACAAAACA CGAAATCGCC GATGCCTGTG ACTTAACCAT CGACGAGATG 
GAGAGTGCGG CCAGCAACCT GCTGAACGGG AACGTGCATC CGCGGGCCAA TGGAGACGTG 
GGCCCCGTTT CCCACCGGCA AGACTACGAG CTCCAGGACT TTGGGCCTGG CTACAGCGAC 
GAGGAGCCGG ACCCCGGGAG GGACGAGGAG GACCTGGCAG ACGAGATGAT TTGCATCACC 
ACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817672.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815974.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X5, mRNA.

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CloneID	OBa45769
Clone ID Related Accession (Same CDS sequence)	XM_010817672.1
Accession Version	XM_010817672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGATTGGTG 
AAGCTTCTCA GCAGGGGGGA AGGCATCCGG ACGCTGCTGT GGACTTTTAT TAAGTCCTTC 
CAGGCGCTCC CGTATGTCGC CCTTCTTATC GCCATGCTGT TCTTCATCTA CGCGGTCATC 
GGCATGCAGA TGTTCGGGAA AGTGGCCATG AGAGATAACA ACCAGATCAA CAGAAACAAC 
AATTTCCAGA CCTTTCCCCA GGCGGTGCTG CTGCTCTTCA GGTGTGCGAC GGGGGAGGCC 
TGGCAGGAAA TCATGTTAGC CTGCCTCCCG GGGAAGCAAT GTGACCCCGA CTCGGATTAC 
AACCCCGGGG AGGAATACAC ATGTGGGAGC AACTTCGCCA TCGTTTATTT CATCAGTTTT 
TATATGCTCT GCGCATTTCT GATCATCAAC CTCTTCGTGG CTGTCATCAT GGACAATTTT 
GATTATCTCA CCCGGGACTG GTCTATTCTG GGGCCTCACC ATTTAGATGA ATTCAAACGG 
ATCTGGTCTG AATATGACCC CGAGGCAAAG GGCAGGATCA AGCACCTGGA CGTGGTCACT 
CTGCTCCGCC GCATCCAGCC TCCCCTGGGC TTCGGGAAGT TGTGTCCACA CCGGGTAGCC 
TGCAAGAGAC TAGTTGCTAT GAACATGCCA CTCAACAGTG ATGGGACAGT CATGTTTAAT 
GCAACTCTGT TTGCTCTGGT TCGAACGGCT CTTAAAATCA AGACTGAAGG CAACCTGGAA 
CAAGCTAATG AAGAACTCCG AGCTGTGATC AAGAAAATTT GGAAGAAAAC CAGCATGAAA 
CTACTTGACC AAGTTGTTCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA ACAAGGACTG 
GTGGGAAAAT ACCCCGCGAA GAACAGCACA ATTGCCCTAC AGGCGGGATT AAGGACACTG 
CATGACATTG GGCCAGAAAT CCGGCGTGCT ATATCATGCG ATTTGCAAGA TGATGATCCA 
GAGGAAACAA AACGAGAAGA CGAAGAAGAT GTGTTCAAAA GAAATGGTGC CCTGCTTGGA 
AACCATGTCA ATCATGTTAA TAGTGATAGG AGAGATTCCC TTCAGCAGAC CAATACCACC 
CACCGTCCCC TGCATGTCCA AAGGCCTTCA ATTCCACCTG CAAGTGATAC TGAGAAACCG 
CTGTTTCCTC CAGCAGGAAA TTCGGTGTGT CATAACCATC ATAACCATAA TTCCATAGGA 
AAGCAAGTTC CCAGCTCAAC AAATGCCAAT CTCAATAATG CCAATATGTG CAAAGCTGCC 
CCTGGAAAGC GGCCCAGCAT TGGGAACCTT GAGCTTGTGT CTGAAAACGG GCATCATTCC 
TTCCACAAGC ATGGCCGTGA GCCTCACAGA AGGTGCAGTA TTAAAAGAAC CCGCTATTAT 
GAAACTTACA TTAGGTCTGA CTCAGGAGAC GAGCAGTTCC CGACGATTTG CCGGGAGGAC 
CCGGAGATAC ACGGCTACTT CAGGGACCCC CACTGCCCAG GGGAGCAGGA GTATTTCAGC 
AGCGAGGAGT ACTATGAGGA TGACAGCTCG CCCACCGGGA GCAGGCAAAG CTACGGCTGC 
GGCTACTATA ACTGGTACCT GGGCAGCAGC TTGGACTTTG AGTGGCCTCG AGGCTACCAT 
CACCCCCAAA GCTTCTCAGA GGACGACGAC TCGCCCGTTG GCTACGATTC CCGGAGGTCT 
CCGAGGCGAC GCCTACTACC TCCCACCCCT ACATCCCCCC GCAGGTCTTC CTTCAACTTC 
GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG GAGGTCCCGC CGTCGCCCGC CTTCCCGCCC 
CGCACGGCCC TGCCTCTGCA CCTGATGCAG CAGCAGATCA TGGCAGTCGC CGGCCTAGAT 
TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC CACTCCACCC GGTCGTGGGC CACGCCCCCG 
GCCACGCCGT CGTATCGGGA CTGGACGCCG TGCTATACCC CGCTGATCCA GGTTGAGCGG 
CCAGAGGCCC CAGACCAGGT CAACGGCAGC CTCCCGTCCC TGCACCGCAG CTCGTGGTAC 
ACGGACGAGC CCAGCGTCTC CTATCGGACG TTCACACCAG CCAGCCTGAC CGTCCCCAGC 
AGCTTCCACA ACAAGAACAG CGACAAGCAG AGGAGCGCGG ACAGCCTGGT GGAGGCAGTT 
CTGATATCCG AAGGCTTAGG ACGGTACGCG AGGGACCCAA AGTTCGTGTC GGCGACAAAA 
CACGAAATCG CCGATGCCTG TGACTTAACC ATCGACGAGA TGGAGAGTGC GGCCAGCAAC 
CTGCTGAACG GGAACGTGCA TCCGCGGGCC AATGGAGACG TGGGCCCCGT TTCCCACCGG 
CAAGACTACG AGCTCCAGGA CTTTGGGCCT GGCTACAGCG ACGAGGAGCC GGACCCCGGG 
AGGGACGAGG AGGACCTGGC AGACGAGATG ATTTGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815974.1
CDS	259..6849
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817672.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817672.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGATTGGTG 
AAGCTTCTCA GCAGGGGGGA AGGCATCCGG ACGCTGCTGT GGACTTTTAT TAAGTCCTTC 
CAGGCGCTCC CGTATGTCGC CCTTCTTATC GCCATGCTGT TCTTCATCTA CGCGGTCATC 
GGCATGCAGA TGTTCGGGAA AGTGGCCATG AGAGATAACA ACCAGATCAA CAGAAACAAC 
AATTTCCAGA CCTTTCCCCA GGCGGTGCTG CTGCTCTTCA GGTGTGCGAC GGGGGAGGCC 
TGGCAGGAAA TCATGTTAGC CTGCCTCCCG GGGAAGCAAT GTGACCCCGA CTCGGATTAC 
AACCCCGGGG AGGAATACAC ATGTGGGAGC AACTTCGCCA TCGTTTATTT CATCAGTTTT 
TATATGCTCT GCGCATTTCT GATCATCAAC CTCTTCGTGG CTGTCATCAT GGACAATTTT 
GATTATCTCA CCCGGGACTG GTCTATTCTG GGGCCTCACC ATTTAGATGA ATTCAAACGG 
ATCTGGTCTG AATATGACCC CGAGGCAAAG GGCAGGATCA AGCACCTGGA CGTGGTCACT 
CTGCTCCGCC GCATCCAGCC TCCCCTGGGC TTCGGGAAGT TGTGTCCACA CCGGGTAGCC 
TGCAAGAGAC TAGTTGCTAT GAACATGCCA CTCAACAGTG ATGGGACAGT CATGTTTAAT 
GCAACTCTGT TTGCTCTGGT TCGAACGGCT CTTAAAATCA AGACTGAAGG CAACCTGGAA 
CAAGCTAATG AAGAACTCCG AGCTGTGATC AAGAAAATTT GGAAGAAAAC CAGCATGAAA 
CTACTTGACC AAGTTGTTCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA ACAAGGACTG 
GTGGGAAAAT ACCCCGCGAA GAACAGCACA ATTGCCCTAC AGGCGGGATT AAGGACACTG 
CATGACATTG GGCCAGAAAT CCGGCGTGCT ATATCATGCG ATTTGCAAGA TGATGATCCA 
GAGGAAACAA AACGAGAAGA CGAAGAAGAT GTGTTCAAAA GAAATGGTGC CCTGCTTGGA 
AACCATGTCA ATCATGTTAA TAGTGATAGG AGAGATTCCC TTCAGCAGAC CAATACCACC 
CACCGTCCCC TGCATGTCCA AAGGCCTTCA ATTCCACCTG CAAGTGATAC TGAGAAACCG 
CTGTTTCCTC CAGCAGGAAA TTCGGTGTGT CATAACCATC ATAACCATAA TTCCATAGGA 
AAGCAAGTTC CCAGCTCAAC AAATGCCAAT CTCAATAATG CCAATATGTG CAAAGCTGCC 
CCTGGAAAGC GGCCCAGCAT TGGGAACCTT GAGCTTGTGT CTGAAAACGG GCATCATTCC 
TTCCACAAGC ATGGCCGTGA GCCTCACAGA AGGTGCAGTA TTAAAAGAAC CCGCTATTAT 
GAAACTTACA TTAGGTCTGA CTCAGGAGAC GAGCAGTTCC CGACGATTTG CCGGGAGGAC 
CCGGAGATAC ACGGCTACTT CAGGGACCCC CACTGCCCAG GGGAGCAGGA GTATTTCAGC 
AGCGAGGAGT ACTATGAGGA TGACAGCTCG CCCACCGGGA GCAGGCAAAG CTACGGCTGC 
GGCTACTATA ACTGGTACCT GGGCAGCAGC TTGGACTTTG AGTGGCCTCG AGGCTACCAT 
CACCCCCAAA GCTTCTCAGA GGACGACGAC TCGCCCGTTG GCTACGATTC CCGGAGGTCT 
CCGAGGCGAC GCCTACTACC TCCCACCCCT ACATCCCCCC GCAGGTCTTC CTTCAACTTC 
GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG GAGGTCCCGC CGTCGCCCGC CTTCCCGCCC 
CGCACGGCCC TGCCTCTGCA CCTGATGCAG CAGCAGATCA TGGCAGTCGC CGGCCTAGAT 
TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC CACTCCACCC GGTCGTGGGC CACGCCCCCG 
GCCACGCCGT CGTATCGGGA CTGGACGCCG TGCTATACCC CGCTGATCCA GGTTGAGCGG 
CCAGAGGCCC CAGACCAGGT CAACGGCAGC CTCCCGTCCC TGCACCGCAG CTCGTGGTAC 
ACGGACGAGC CCAGCGTCTC CTATCGGACG TTCACACCAG CCAGCCTGAC CGTCCCCAGC 
AGCTTCCACA ACAAGAACAG CGACAAGCAG AGGAGCGCGG ACAGCCTGGT GGAGGCAGTT 
CTGATATCCG AAGGCTTAGG ACGGTACGCG AGGGACCCAA AGTTCGTGTC GGCGACAAAA 
CACGAAATCG CCGATGCCTG TGACTTAACC ATCGACGAGA TGGAGAGTGC GGCCAGCAAC 
CTGCTGAACG GGAACGTGCA TCCGCGGGCC AATGGAGACG TGGGCCCCGT TTCCCACCGG 
CAAGACTACG AGCTCCAGGA CTTTGGGCCT GGCTACAGCG ACGAGGAGCC GGACCCCGGG 
AGGGACGAGG AGGACCTGGC AGACGAGATG ATTTGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817673.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815975.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X6, mRNA.

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CloneID	OBa45770
Clone ID Related Accession (Same CDS sequence)	XM_010817673.1
Accession Version	XM_010817673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA TTCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAATTCC ACCTGCAAGT GATACTGAGA AACCGCTGTT TCCTCCAGCA 
GGAAATTCGG TGTGTCATAA CCATCATAAC CATAATTCCA TAGGAAAGCA AGTTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTGCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCT TGTGTCTGAA AACGGGCATC ATTCCTTCCA CAAGCATGGC 
CGTGAGCCTC ACAGAAGGTG CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGG 
TCTGACTCAG GAGACGAGCA GTTCCCGACG ATTTGCCGGG AGGACCCGGA GATACACGGC 
TACTTCAGGG ACCCCCACTG CCCAGGGGAG CAGGAGTATT TCAGCAGCGA GGAGTACTAT 
GAGGATGACA GCTCGCCCAC CGGGAGCAGG CAAAGCTACG GCTGCGGCTA CTATAACTGG 
TACCTGGGCA GCAGCTTGGA CTTTGAGTGG CCTCGAGGCT ACCATCACCC CCAAAGCTTC 
TCAGAGGACG ACGACTCGCC CGTTGGCTAC GATTCCCGGA GGTCTCCGAG GCGACGCCTA 
CTACCTCCCA CCCCTACATC CCCCCGCAGG TCTTCCTTCA ACTTCGAGTG CCTGCGCCGG 
CAGAGCAGCC AGGAGGAGGT CCCGCCGTCG CCCGCCTTCC CGCCCCGCAC GGCCCTGCCT 
CTGCACCTGA TGCAGCAGCA GATCATGGCA GTCGCCGGCC TAGATTCCAG TAAAGCCCAG 
AAGTACTCGC CGAGCCACTC CACCCGGTCG TGGGCCACGC CCCCGGCCAC GCCGTCGTAT 
CGGGACTGGA CGCCGTGCTA TACCCCGCTG ATCCAGGTTG AGCGGCCAGA GGCCCCAGAC 
CAGGTCAACG GCAGCCTCCC GTCCCTGCAC CGCAGCTCGT GGTACACGGA CGAGCCCAGC 
GTCTCCTATC GGACGTTCAC ACCAGCCAGC CTGACCGTCC CCAGCAGCTT CCACAACAAG 
AACAGCGACA AGCAGAGGAG CGCGGACAGC CTGGTGGAGG CAGTTCTGAT ATCCGAAGGC 
TTAGGACGGT ACGCGAGGGA CCCAAAGTTC GTGTCGGCGA CAAAACACGA AATCGCCGAT 
GCCTGTGACT TAACCATCGA CGAGATGGAG AGTGCGGCCA GCAACCTGCT GAACGGGAAC 
GTGCATCCGC GGGCCAATGG AGACGTGGGC CCCGTTTCCC ACCGGCAAGA CTACGAGCTC 
CAGGACTTTG GGCCTGGCTA CAGCGACGAG GAGCCGGACC CCGGGAGGGA CGAGGAGGAC 
CTGGCAGACG AGATGATTTG CATCACCACC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815975.1
CDS	259..6834
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESESVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817673.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817673.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA TTCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAATTCC ACCTGCAAGT GATACTGAGA AACCGCTGTT TCCTCCAGCA 
GGAAATTCGG TGTGTCATAA CCATCATAAC CATAATTCCA TAGGAAAGCA AGTTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTGCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCT TGTGTCTGAA AACGGGCATC ATTCCTTCCA CAAGCATGGC 
CGTGAGCCTC ACAGAAGGTG CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGG 
TCTGACTCAG GAGACGAGCA GTTCCCGACG ATTTGCCGGG AGGACCCGGA GATACACGGC 
TACTTCAGGG ACCCCCACTG CCCAGGGGAG CAGGAGTATT TCAGCAGCGA GGAGTACTAT 
GAGGATGACA GCTCGCCCAC CGGGAGCAGG CAAAGCTACG GCTGCGGCTA CTATAACTGG 
TACCTGGGCA GCAGCTTGGA CTTTGAGTGG CCTCGAGGCT ACCATCACCC CCAAAGCTTC 
TCAGAGGACG ACGACTCGCC CGTTGGCTAC GATTCCCGGA GGTCTCCGAG GCGACGCCTA 
CTACCTCCCA CCCCTACATC CCCCCGCAGG TCTTCCTTCA ACTTCGAGTG CCTGCGCCGG 
CAGAGCAGCC AGGAGGAGGT CCCGCCGTCG CCCGCCTTCC CGCCCCGCAC GGCCCTGCCT 
CTGCACCTGA TGCAGCAGCA GATCATGGCA GTCGCCGGCC TAGATTCCAG TAAAGCCCAG 
AAGTACTCGC CGAGCCACTC CACCCGGTCG TGGGCCACGC CCCCGGCCAC GCCGTCGTAT 
CGGGACTGGA CGCCGTGCTA TACCCCGCTG ATCCAGGTTG AGCGGCCAGA GGCCCCAGAC 
CAGGTCAACG GCAGCCTCCC GTCCCTGCAC CGCAGCTCGT GGTACACGGA CGAGCCCAGC 
GTCTCCTATC GGACGTTCAC ACCAGCCAGC CTGACCGTCC CCAGCAGCTT CCACAACAAG 
AACAGCGACA AGCAGAGGAG CGCGGACAGC CTGGTGGAGG CAGTTCTGAT ATCCGAAGGC 
TTAGGACGGT ACGCGAGGGA CCCAAAGTTC GTGTCGGCGA CAAAACACGA AATCGCCGAT 
GCCTGTGACT TAACCATCGA CGAGATGGAG AGTGCGGCCA GCAACCTGCT GAACGGGAAC 
GTGCATCCGC GGGCCAATGG AGACGTGGGC CCCGTTTCCC ACCGGCAAGA CTACGAGCTC 
CAGGACTTTG GGCCTGGCTA CAGCGACGAG GAGCCGGACC CCGGGAGGGA CGAGGAGGAC 
CTGGCAGACG AGATGATTTG CATCACCACC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817674.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815976.1 Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X1, mRNA.

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CloneID	OBa45771
Clone ID Related Accession (Same CDS sequence)	XM_010817674.2 , XM_010817674.1
Accession Version	XM_010817674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCAACTGAAA GTGAAAGTGT CCCTGTCCCA 
ACCGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACGCTGCTG 
TGGACTTTTA TTAAGTCCTT CCAGGCGCTC CCGTATGTCG CCCTTCTTAT CGCCATGCTG 
TTCTTCATCT ACGCGGTCAT CGGCATGCAG ATGTTCGGGA AAGTGGCCAT GAGAGATAAC 
AACCAGATCA ACAGAAACAA CAATTTCCAG ACCTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCGA CGGGGGAGGC CTGGCAGGAA ATCATGTTAG CCTGCCTCCC GGGGAAGCAA 
TGTGACCCCG ACTCGGATTA CAACCCCGGG GAGGAATACA CATGTGGGAG CAACTTCGCC 
ATCGTTTATT TCATCAGTTT TTATATGCTC TGCGCATTTC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGATTATCTC ACCCGGGACT GGTCTATTCT GGGGCCTCAC 
CATTTAGATG AATTCAAACG GATCTGGTCT GAATATGACC CCGAGGCAAA GGGCAGGATC 
AAGCACCTGG ACGTGGTCAC TCTGCTCCGC CGCATCCAGC CTCCCCTGGG CTTCGGGAAG 
TTGTGTCCAC ACCGGGTAGC CTGCAAGAGA CTAGTTGCTA TGAACATGCC ACTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACTCTG TTTGCTCTGG TTCGAACGGC TCTTAAAATC 
AAGACTGAAG GCAACCTGGA ACAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ACTACTTGAC CAAGTTGTTC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCCGCGA AGAACAGCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGC 
GATTTGCAAG ATGATGATCC AGAGGAAACA AAACGAGAAG ACGAAGAAGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT GCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCTTGTG 
TCTGAAAACG GGCATCATTC CTTCCACAAG CATGGCCGTG AGCCTCACAG AAGGTGCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCTG ACTCAGGAGA CGAGCAGTTC 
CCGACGATTT GCCGGGAGGA CCCGGAGATA CACGGCTACT TCAGGGACCC CCACTGCCCA 
GGGGAGCAGG AGTATTTCAG CAGCGAGGAG TACTATGAGG ATGACAGCTC GCCCACCGGG 
AGCAGGCAAA GCTACGGCTG CGGCTACTAT AACTGGTACC TGGGCAGCAG CTTGGACTTT 
GAGTGGCCTC GAGGCTACCA TCACCCCCAA AGCTTCTCAG AGGACGACGA CTCGCCCGTT 
GGCTACGATT CCCGGAGGTC TCCGAGGCGA CGCCTACTAC CTCCCACCCC TACATCCCCC 
CGCAGGTCTT CCTTCAACTT CGAGTGCCTG CGCCGGCAGA GCAGCCAGGA GGAGGTCCCG 
CCGTCGCCCG CCTTCCCGCC CCGCACGGCC CTGCCTCTGC ACCTGATGCA GCAGCAGATC 
ATGGCAGTCG CCGGCCTAGA TTCCAGTAAA GCCCAGAAGT ACTCGCCGAG CCACTCCACC 
CGGTCGTGGG CCACGCCCCC GGCCACGCCG TCGTATCGGG ACTGGACGCC GTGCTATACC 
CCGCTGATCC AGGTTGAGCG GCCAGAGGCC CCAGACCAGG TCAACGGCAG CCTCCCGTCC 
CTGCACCGCA GCTCGTGGTA CACGGACGAG CCCAGCGTCT CCTATCGGAC GTTCACACCA 
GCCAGCCTGA CCGTCCCCAG CAGCTTCCAC AACAAGAACA GCGACAAGCA GAGGAGCGCG 
GACAGCCTGG TGGAGGCAGT TCTGATATCC GAAGGCTTAG GACGGTACGC GAGGGACCCA 
AAGTTCGTGT CGGCGACAAA ACACGAAATC GCCGATGCCT GTGACTTAAC CATCGACGAG 
ATGGAGAGTG CGGCCAGCAA CCTGCTGAAC GGGAACGTGC ATCCGCGGGC CAATGGAGAC 
GTGGGCCCCG TTTCCCACCG GCAAGACTAC GAGCTCCAGG ACTTTGGGCC TGGCTACAGC 
GACGAGGAGC CGGACCCCGG GAGGGACGAG GAGGACCTGG CAGACGAGAT GATTTGCATC 
ACCACCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815976.1
CDS	376..6927
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPTESESVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817674.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817674.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCAACTGAAA GTGAAAGTGT CCCTGTCCCA 
ACCGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACGCTGCTG 
TGGACTTTTA TTAAGTCCTT CCAGGCGCTC CCGTATGTCG CCCTTCTTAT CGCCATGCTG 
TTCTTCATCT ACGCGGTCAT CGGCATGCAG ATGTTCGGGA AAGTGGCCAT GAGAGATAAC 
AACCAGATCA ACAGAAACAA CAATTTCCAG ACCTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCGA CGGGGGAGGC CTGGCAGGAA ATCATGTTAG CCTGCCTCCC GGGGAAGCAA 
TGTGACCCCG ACTCGGATTA CAACCCCGGG GAGGAATACA CATGTGGGAG CAACTTCGCC 
ATCGTTTATT TCATCAGTTT TTATATGCTC TGCGCATTTC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGATTATCTC ACCCGGGACT GGTCTATTCT GGGGCCTCAC 
CATTTAGATG AATTCAAACG GATCTGGTCT GAATATGACC CCGAGGCAAA GGGCAGGATC 
AAGCACCTGG ACGTGGTCAC TCTGCTCCGC CGCATCCAGC CTCCCCTGGG CTTCGGGAAG 
TTGTGTCCAC ACCGGGTAGC CTGCAAGAGA CTAGTTGCTA TGAACATGCC ACTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACTCTG TTTGCTCTGG TTCGAACGGC TCTTAAAATC 
AAGACTGAAG GCAACCTGGA ACAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ACTACTTGAC CAAGTTGTTC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCCGCGA AGAACAGCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGC 
GATTTGCAAG ATGATGATCC AGAGGAAACA AAACGAGAAG ACGAAGAAGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT GCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCTTGTG 
TCTGAAAACG GGCATCATTC CTTCCACAAG CATGGCCGTG AGCCTCACAG AAGGTGCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCTG ACTCAGGAGA CGAGCAGTTC 
CCGACGATTT GCCGGGAGGA CCCGGAGATA CACGGCTACT TCAGGGACCC CCACTGCCCA 
GGGGAGCAGG AGTATTTCAG CAGCGAGGAG TACTATGAGG ATGACAGCTC GCCCACCGGG 
AGCAGGCAAA GCTACGGCTG CGGCTACTAT AACTGGTACC TGGGCAGCAG CTTGGACTTT 
GAGTGGCCTC GAGGCTACCA TCACCCCCAA AGCTTCTCAG AGGACGACGA CTCGCCCGTT 
GGCTACGATT CCCGGAGGTC TCCGAGGCGA CGCCTACTAC CTCCCACCCC TACATCCCCC 
CGCAGGTCTT CCTTCAACTT CGAGTGCCTG CGCCGGCAGA GCAGCCAGGA GGAGGTCCCG 
CCGTCGCCCG CCTTCCCGCC CCGCACGGCC CTGCCTCTGC ACCTGATGCA GCAGCAGATC 
ATGGCAGTCG CCGGCCTAGA TTCCAGTAAA GCCCAGAAGT ACTCGCCGAG CCACTCCACC 
CGGTCGTGGG CCACGCCCCC GGCCACGCCG TCGTATCGGG ACTGGACGCC GTGCTATACC 
CCGCTGATCC AGGTTGAGCG GCCAGAGGCC CCAGACCAGG TCAACGGCAG CCTCCCGTCC 
CTGCACCGCA GCTCGTGGTA CACGGACGAG CCCAGCGTCT CCTATCGGAC GTTCACACCA 
GCCAGCCTGA CCGTCCCCAG CAGCTTCCAC AACAAGAACA GCGACAAGCA GAGGAGCGCG 
GACAGCCTGG TGGAGGCAGT TCTGATATCC GAAGGCTTAG GACGGTACGC GAGGGACCCA 
AAGTTCGTGT CGGCGACAAA ACACGAAATC GCCGATGCCT GTGACTTAAC CATCGACGAG 
ATGGAGAGTG CGGCCAGCAA CCTGCTGAAC GGGAACGTGC ATCCGCGGGC CAATGGAGAC 
GTGGGCCCCG TTTCCCACCG GCAAGACTAC GAGCTCCAGG ACTTTGGGCC TGGCTACAGC 
GACGAGGAGC CGGACCCCGG GAGGGACGAG GAGGACCTGG CAGACGAGAT GATTTGCATC 
ACCACCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817674.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815976.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X8, mRNA.

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CloneID	OBa45771
Clone ID Related Accession (Same CDS sequence)	XM_010817674.2 , XM_010817674.1
Accession Version	XM_010817674.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCAACTGAAA GTGAAAGTGT CCCTGTCCCA 
ACCGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACGCTGCTG 
TGGACTTTTA TTAAGTCCTT CCAGGCGCTC CCGTATGTCG CCCTTCTTAT CGCCATGCTG 
TTCTTCATCT ACGCGGTCAT CGGCATGCAG ATGTTCGGGA AAGTGGCCAT GAGAGATAAC 
AACCAGATCA ACAGAAACAA CAATTTCCAG ACCTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCGA CGGGGGAGGC CTGGCAGGAA ATCATGTTAG CCTGCCTCCC GGGGAAGCAA 
TGTGACCCCG ACTCGGATTA CAACCCCGGG GAGGAATACA CATGTGGGAG CAACTTCGCC 
ATCGTTTATT TCATCAGTTT TTATATGCTC TGCGCATTTC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGATTATCTC ACCCGGGACT GGTCTATTCT GGGGCCTCAC 
CATTTAGATG AATTCAAACG GATCTGGTCT GAATATGACC CCGAGGCAAA GGGCAGGATC 
AAGCACCTGG ACGTGGTCAC TCTGCTCCGC CGCATCCAGC CTCCCCTGGG CTTCGGGAAG 
TTGTGTCCAC ACCGGGTAGC CTGCAAGAGA CTAGTTGCTA TGAACATGCC ACTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACTCTG TTTGCTCTGG TTCGAACGGC TCTTAAAATC 
AAGACTGAAG GCAACCTGGA ACAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ACTACTTGAC CAAGTTGTTC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCCGCGA AGAACAGCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGC 
GATTTGCAAG ATGATGATCC AGAGGAAACA AAACGAGAAG ACGAAGAAGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT GCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCTTGTG 
TCTGAAAACG GGCATCATTC CTTCCACAAG CATGGCCGTG AGCCTCACAG AAGGTGCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCTG ACTCAGGAGA CGAGCAGTTC 
CCGACGATTT GCCGGGAGGA CCCGGAGATA CACGGCTACT TCAGGGACCC CCACTGCCCA 
GGGGAGCAGG AGTATTTCAG CAGCGAGGAG TACTATGAGG ATGACAGCTC GCCCACCGGG 
AGCAGGCAAA GCTACGGCTG CGGCTACTAT AACTGGTACC TGGGCAGCAG CTTGGACTTT 
GAGTGGCCTC GAGGCTACCA TCACCCCCAA AGCTTCTCAG AGGACGACGA CTCGCCCGTT 
GGCTACGATT CCCGGAGGTC TCCGAGGCGA CGCCTACTAC CTCCCACCCC TACATCCCCC 
CGCAGGTCTT CCTTCAACTT CGAGTGCCTG CGCCGGCAGA GCAGCCAGGA GGAGGTCCCG 
CCGTCGCCCG CCTTCCCGCC CCGCACGGCC CTGCCTCTGC ACCTGATGCA GCAGCAGATC 
ATGGCAGTCG CCGGCCTAGA TTCCAGTAAA GCCCAGAAGT ACTCGCCGAG CCACTCCACC 
CGGTCGTGGG CCACGCCCCC GGCCACGCCG TCGTATCGGG ACTGGACGCC GTGCTATACC 
CCGCTGATCC AGGTTGAGCG GCCAGAGGCC CCAGACCAGG TCAACGGCAG CCTCCCGTCC 
CTGCACCGCA GCTCGTGGTA CACGGACGAG CCCAGCGTCT CCTATCGGAC GTTCACACCA 
GCCAGCCTGA CCGTCCCCAG CAGCTTCCAC AACAAGAACA GCGACAAGCA GAGGAGCGCG 
GACAGCCTGG TGGAGGCAGT TCTGATATCC GAAGGCTTAG GACGGTACGC GAGGGACCCA 
AAGTTCGTGT CGGCGACAAA ACACGAAATC GCCGATGCCT GTGACTTAAC CATCGACGAG 
ATGGAGAGTG CGGCCAGCAA CCTGCTGAAC GGGAACGTGC ATCCGCGGGC CAATGGAGAC 
GTGGGCCCCG TTTCCCACCG GCAAGACTAC GAGCTCCAGG ACTTTGGGCC TGGCTACAGC 
GACGAGGAGC CGGACCCCGG GAGGGACGAG GAGGACCTGG CAGACGAGAT GATTTGCATC 
ACCACCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815976.1
CDS	259..6810
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPTESESVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817674.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817674.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCAACTGAAA GTGAAAGTGT CCCTGTCCCA 
ACCGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACGCTGCTG 
TGGACTTTTA TTAAGTCCTT CCAGGCGCTC CCGTATGTCG CCCTTCTTAT CGCCATGCTG 
TTCTTCATCT ACGCGGTCAT CGGCATGCAG ATGTTCGGGA AAGTGGCCAT GAGAGATAAC 
AACCAGATCA ACAGAAACAA CAATTTCCAG ACCTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCGA CGGGGGAGGC CTGGCAGGAA ATCATGTTAG CCTGCCTCCC GGGGAAGCAA 
TGTGACCCCG ACTCGGATTA CAACCCCGGG GAGGAATACA CATGTGGGAG CAACTTCGCC 
ATCGTTTATT TCATCAGTTT TTATATGCTC TGCGCATTTC TGATCATCAA CCTCTTCGTG 
GCTGTCATCA TGGACAATTT TGATTATCTC ACCCGGGACT GGTCTATTCT GGGGCCTCAC 
CATTTAGATG AATTCAAACG GATCTGGTCT GAATATGACC CCGAGGCAAA GGGCAGGATC 
AAGCACCTGG ACGTGGTCAC TCTGCTCCGC CGCATCCAGC CTCCCCTGGG CTTCGGGAAG 
TTGTGTCCAC ACCGGGTAGC CTGCAAGAGA CTAGTTGCTA TGAACATGCC ACTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACTCTG TTTGCTCTGG TTCGAACGGC TCTTAAAATC 
AAGACTGAAG GCAACCTGGA ACAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ACTACTTGAC CAAGTTGTTC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCCGCGA AGAACAGCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGC 
GATTTGCAAG ATGATGATCC AGAGGAAACA AAACGAGAAG ACGAAGAAGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT GCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCTTGTG 
TCTGAAAACG GGCATCATTC CTTCCACAAG CATGGCCGTG AGCCTCACAG AAGGTGCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCTG ACTCAGGAGA CGAGCAGTTC 
CCGACGATTT GCCGGGAGGA CCCGGAGATA CACGGCTACT TCAGGGACCC CCACTGCCCA 
GGGGAGCAGG AGTATTTCAG CAGCGAGGAG TACTATGAGG ATGACAGCTC GCCCACCGGG 
AGCAGGCAAA GCTACGGCTG CGGCTACTAT AACTGGTACC TGGGCAGCAG CTTGGACTTT 
GAGTGGCCTC GAGGCTACCA TCACCCCCAA AGCTTCTCAG AGGACGACGA CTCGCCCGTT 
GGCTACGATT CCCGGAGGTC TCCGAGGCGA CGCCTACTAC CTCCCACCCC TACATCCCCC 
CGCAGGTCTT CCTTCAACTT CGAGTGCCTG CGCCGGCAGA GCAGCCAGGA GGAGGTCCCG 
CCGTCGCCCG CCTTCCCGCC CCGCACGGCC CTGCCTCTGC ACCTGATGCA GCAGCAGATC 
ATGGCAGTCG CCGGCCTAGA TTCCAGTAAA GCCCAGAAGT ACTCGCCGAG CCACTCCACC 
CGGTCGTGGG CCACGCCCCC GGCCACGCCG TCGTATCGGG ACTGGACGCC GTGCTATACC 
CCGCTGATCC AGGTTGAGCG GCCAGAGGCC CCAGACCAGG TCAACGGCAG CCTCCCGTCC 
CTGCACCGCA GCTCGTGGTA CACGGACGAG CCCAGCGTCT CCTATCGGAC GTTCACACCA 
GCCAGCCTGA CCGTCCCCAG CAGCTTCCAC AACAAGAACA GCGACAAGCA GAGGAGCGCG 
GACAGCCTGG TGGAGGCAGT TCTGATATCC GAAGGCTTAG GACGGTACGC GAGGGACCCA 
AAGTTCGTGT CGGCGACAAA ACACGAAATC GCCGATGCCT GTGACTTAAC CATCGACGAG 
ATGGAGAGTG CGGCCAGCAA CCTGCTGAAC GGGAACGTGC ATCCGCGGGC CAATGGAGAC 
GTGGGCCCCG TTTCCCACCG GCAAGACTAC GAGCTCCAGG ACTTTGGGCC TGGCTACAGC 
GACGAGGAGC CGGACCCCGG GAGGGACGAG GAGGACCTGG CAGACGAGAT GATTTGCATC 
ACCACCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817676.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815978.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X9, mRNA.

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CloneID	OBa45773
Clone ID Related Accession (Same CDS sequence)	XM_010817676.1 , XM_010817676.2
Accession Version	XM_010817676.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815978.1
CDS	259..6765
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817676.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817676.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817676.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815978.1 Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X2, mRNA.

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CloneID	OBa45773
Clone ID Related Accession (Same CDS sequence)	XM_010817676.1 , XM_010817676.2
Accession Version	XM_010817676.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817676.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815978.1
CDS	375..6881
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817676.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817676.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817677.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815979.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X10, mRNA.

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CloneID	OBa45774
Clone ID Related Accession (Same CDS sequence)	XM_010817677.1 , XM_010817677.2
Accession Version	XM_010817677.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815979.1
CDS	259..6765
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817677.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817677.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817677.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815979.1 Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X3, mRNA.

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CloneID	OBa45774
Clone ID Related Accession (Same CDS sequence)	XM_010817677.1 , XM_010817677.2
Accession Version	XM_010817677.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815979.1
CDS	375..6881
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817677.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817677.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817678.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815980.1 Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X5, mRNA.

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CloneID	OBa45775
Clone ID Related Accession (Same CDS sequence)	XM_010817678.2 , XM_010817678.1
Accession Version	XM_010817678.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGATTGG TGAAGCTTCT CAGCAGGGGG GAAGGCATCC GGACGCTGCT GTGGACTTTT 
ATTAAGTCCT TCCAGGCGCT CCCGTATGTC GCCCTTCTTA TCGCCATGCT GTTCTTCATC 
TACGCGGTCA TCGGCATGCA GATGTTCGGG AAAGTGGCCA TGAGAGATAA CAACCAGATC 
AACAGAAACA ACAATTTCCA GACCTTTCCC CAGGCGGTGC TGCTGCTCTT CAGGTGTGCG 
ACGGGGGAGG CCTGGCAGGA AATCATGTTA GCCTGCCTCC CGGGGAAGCA ATGTGACCCC 
GACTCGGATT ACAACCCCGG GGAGGAATAC ACATGTGGGA GCAACTTCGC CATCGTTTAT 
TTCATCAGTT TTTATATGCT CTGCGCATTT CTGATCATCA ACCTCTTCGT GGCTGTCATC 
ATGGACAATT TTGATTATCT CACCCGGGAC TGGTCTATTC TGGGGCCTCA CCATTTAGAT 
GAATTCAAAC GGATCTGGTC TGAATATGAC CCCGAGGCAA AGGGCAGGAT CAAGCACCTG 
GACGTGGTCA CTCTGCTCCG CCGCATCCAG CCTCCCCTGG GCTTCGGGAA GTTGTGTCCA 
CACCGGGTAG CCTGCAAGAG ACTAGTTGCT ATGAACATGC CACTCAACAG TGATGGGACA 
GTCATGTTTA ATGCAACTCT GTTTGCTCTG GTTCGAACGG CTCTTAAAAT CAAGACTGAA 
GGCAACCTGG AACAAGCTAA TGAAGAACTC CGAGCTGTGA TCAAGAAAAT TTGGAAGAAA 
ACCAGCATGA AACTACTTGA CCAAGTTGTT CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAACAAGGAC TGGTGGGAAA ATACCCCGCG AAGAACAGCA CAATTGCCCT ACAGGCGGGA 
TTAAGGACAC TGCATGACAT TGGGCCAGAA ATCCGGCGTG CTATATCATG CGATTTGCAA 
GATGATGATC CAGAGGAAAC AAAACGAGAA GACGAAGAAG ATGTGTTCAA AAGAAATGGT 
GCCCTGCTTG GAAACCATGT CAATCATGTT AATAGTGATA GGAGAGATTC CCTTCAGCAG 
ACCAATACCA CCCACCGTCC CCTGCATGTC CAAAGGCCTT CAATTCCACC TGCAAGTGAT 
ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA AATTCGGTGT GTCATAACCA TCATAACCAT 
AATTCCATAG GAAAGCAAGT TCCCAGCTCA ACAAATGCCA ATCTCAATAA TGCCAATATG 
TGCAAAGCTG CCCCTGGAAA GCGGCCCAGC ATTGGGAACC TTGAGCTTGT GTCTGAAAAC 
GGGCATCATT CCTTCCACAA GCATGGCCGT GAGCCTCACA GAAGGTGCAG TATTAAAAGA 
ACCCGCTATT ATGAAACTTA CATTAGGTCT GACTCAGGAG ACGAGCAGTT CCCGACGATT 
TGCCGGGAGG ACCCGGAGAT ACACGGCTAC TTCAGGGACC CCCACTGCCC AGGGGAGCAG 
GAGTATTTCA GCAGCGAGGA GTACTATGAG GATGACAGCT CGCCCACCGG GAGCAGGCAA 
AGCTACGGCT GCGGCTACTA TAACTGGTAC CTGGGCAGCA GCTTGGACTT TGAGTGGCCT 
CGAGGCTACC ATCACCCCCA AAGCTTCTCA GAGGACGACG ACTCGCCCGT TGGCTACGAT 
TCCCGGAGGT CTCCGAGGCG ACGCCTACTA CCTCCCACCC CTACATCCCC CCGCAGGTCT 
TCCTTCAACT TCGAGTGCCT GCGCCGGCAG AGCAGCCAGG AGGAGGTCCC GCCGTCGCCC 
GCCTTCCCGC CCCGCACGGC CCTGCCTCTG CACCTGATGC AGCAGCAGAT CATGGCAGTC 
GCCGGCCTAG ATTCCAGTAA AGCCCAGAAG TACTCGCCGA GCCACTCCAC CCGGTCGTGG 
GCCACGCCCC CGGCCACGCC GTCGTATCGG GACTGGACGC CGTGCTATAC CCCGCTGATC 
CAGGTTGAGC GGCCAGAGGC CCCAGACCAG GTCAACGGCA GCCTCCCGTC CCTGCACCGC 
AGCTCGTGGT ACACGGACGA GCCCAGCGTC TCCTATCGGA CGTTCACACC AGCCAGCCTG 
ACCGTCCCCA GCAGCTTCCA CAACAAGAAC AGCGACAAGC AGAGGAGCGC GGACAGCCTG 
GTGGAGGCAG TTCTGATATC CGAAGGCTTA GGACGGTACG CGAGGGACCC AAAGTTCGTG 
TCGGCGACAA AACACGAAAT CGCCGATGCC TGTGACTTAA CCATCGACGA GATGGAGAGT 
GCGGCCAGCA ACCTGCTGAA CGGGAACGTG CATCCGCGGG CCAATGGAGA CGTGGGCCCC 
GTTTCCCACC GGCAAGACTA CGAGCTCCAG GACTTTGGGC CTGGCTACAG CGACGAGGAG 
CCGGACCCCG GGAGGGACGA GGAGGACCTG GCAGACGAGA TGATTTGCAT CACCACCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815980.1
CDS	375..6797
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817678.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817678.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGATTGG TGAAGCTTCT CAGCAGGGGG GAAGGCATCC GGACGCTGCT GTGGACTTTT 
ATTAAGTCCT TCCAGGCGCT CCCGTATGTC GCCCTTCTTA TCGCCATGCT GTTCTTCATC 
TACGCGGTCA TCGGCATGCA GATGTTCGGG AAAGTGGCCA TGAGAGATAA CAACCAGATC 
AACAGAAACA ACAATTTCCA GACCTTTCCC CAGGCGGTGC TGCTGCTCTT CAGGTGTGCG 
ACGGGGGAGG CCTGGCAGGA AATCATGTTA GCCTGCCTCC CGGGGAAGCA ATGTGACCCC 
GACTCGGATT ACAACCCCGG GGAGGAATAC ACATGTGGGA GCAACTTCGC CATCGTTTAT 
TTCATCAGTT TTTATATGCT CTGCGCATTT CTGATCATCA ACCTCTTCGT GGCTGTCATC 
ATGGACAATT TTGATTATCT CACCCGGGAC TGGTCTATTC TGGGGCCTCA CCATTTAGAT 
GAATTCAAAC GGATCTGGTC TGAATATGAC CCCGAGGCAA AGGGCAGGAT CAAGCACCTG 
GACGTGGTCA CTCTGCTCCG CCGCATCCAG CCTCCCCTGG GCTTCGGGAA GTTGTGTCCA 
CACCGGGTAG CCTGCAAGAG ACTAGTTGCT ATGAACATGC CACTCAACAG TGATGGGACA 
GTCATGTTTA ATGCAACTCT GTTTGCTCTG GTTCGAACGG CTCTTAAAAT CAAGACTGAA 
GGCAACCTGG AACAAGCTAA TGAAGAACTC CGAGCTGTGA TCAAGAAAAT TTGGAAGAAA 
ACCAGCATGA AACTACTTGA CCAAGTTGTT CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAACAAGGAC TGGTGGGAAA ATACCCCGCG AAGAACAGCA CAATTGCCCT ACAGGCGGGA 
TTAAGGACAC TGCATGACAT TGGGCCAGAA ATCCGGCGTG CTATATCATG CGATTTGCAA 
GATGATGATC CAGAGGAAAC AAAACGAGAA GACGAAGAAG ATGTGTTCAA AAGAAATGGT 
GCCCTGCTTG GAAACCATGT CAATCATGTT AATAGTGATA GGAGAGATTC CCTTCAGCAG 
ACCAATACCA CCCACCGTCC CCTGCATGTC CAAAGGCCTT CAATTCCACC TGCAAGTGAT 
ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA AATTCGGTGT GTCATAACCA TCATAACCAT 
AATTCCATAG GAAAGCAAGT TCCCAGCTCA ACAAATGCCA ATCTCAATAA TGCCAATATG 
TGCAAAGCTG CCCCTGGAAA GCGGCCCAGC ATTGGGAACC TTGAGCTTGT GTCTGAAAAC 
GGGCATCATT CCTTCCACAA GCATGGCCGT GAGCCTCACA GAAGGTGCAG TATTAAAAGA 
ACCCGCTATT ATGAAACTTA CATTAGGTCT GACTCAGGAG ACGAGCAGTT CCCGACGATT 
TGCCGGGAGG ACCCGGAGAT ACACGGCTAC TTCAGGGACC CCCACTGCCC AGGGGAGCAG 
GAGTATTTCA GCAGCGAGGA GTACTATGAG GATGACAGCT CGCCCACCGG GAGCAGGCAA 
AGCTACGGCT GCGGCTACTA TAACTGGTAC CTGGGCAGCA GCTTGGACTT TGAGTGGCCT 
CGAGGCTACC ATCACCCCCA AAGCTTCTCA GAGGACGACG ACTCGCCCGT TGGCTACGAT 
TCCCGGAGGT CTCCGAGGCG ACGCCTACTA CCTCCCACCC CTACATCCCC CCGCAGGTCT 
TCCTTCAACT TCGAGTGCCT GCGCCGGCAG AGCAGCCAGG AGGAGGTCCC GCCGTCGCCC 
GCCTTCCCGC CCCGCACGGC CCTGCCTCTG CACCTGATGC AGCAGCAGAT CATGGCAGTC 
GCCGGCCTAG ATTCCAGTAA AGCCCAGAAG TACTCGCCGA GCCACTCCAC CCGGTCGTGG 
GCCACGCCCC CGGCCACGCC GTCGTATCGG GACTGGACGC CGTGCTATAC CCCGCTGATC 
CAGGTTGAGC GGCCAGAGGC CCCAGACCAG GTCAACGGCA GCCTCCCGTC CCTGCACCGC 
AGCTCGTGGT ACACGGACGA GCCCAGCGTC TCCTATCGGA CGTTCACACC AGCCAGCCTG 
ACCGTCCCCA GCAGCTTCCA CAACAAGAAC AGCGACAAGC AGAGGAGCGC GGACAGCCTG 
GTGGAGGCAG TTCTGATATC CGAAGGCTTA GGACGGTACG CGAGGGACCC AAAGTTCGTG 
TCGGCGACAA AACACGAAAT CGCCGATGCC TGTGACTTAA CCATCGACGA GATGGAGAGT 
GCGGCCAGCA ACCTGCTGAA CGGGAACGTG CATCCGCGGG CCAATGGAGA CGTGGGCCCC 
GTTTCCCACC GGCAAGACTA CGAGCTCCAG GACTTTGGGC CTGGCTACAG CGACGAGGAG 
CCGGACCCCG GGAGGGACGA GGAGGACCTG GCAGACGAGA TGATTTGCAT CACCACCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817678.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815980.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X13, mRNA.

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CloneID	OBa45775
Clone ID Related Accession (Same CDS sequence)	XM_010817678.2 , XM_010817678.1
Accession Version	XM_010817678.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGATTGG TGAAGCTTCT CAGCAGGGGG GAAGGCATCC GGACGCTGCT GTGGACTTTT 
ATTAAGTCCT TCCAGGCGCT CCCGTATGTC GCCCTTCTTA TCGCCATGCT GTTCTTCATC 
TACGCGGTCA TCGGCATGCA GATGTTCGGG AAAGTGGCCA TGAGAGATAA CAACCAGATC 
AACAGAAACA ACAATTTCCA GACCTTTCCC CAGGCGGTGC TGCTGCTCTT CAGGTGTGCG 
ACGGGGGAGG CCTGGCAGGA AATCATGTTA GCCTGCCTCC CGGGGAAGCA ATGTGACCCC 
GACTCGGATT ACAACCCCGG GGAGGAATAC ACATGTGGGA GCAACTTCGC CATCGTTTAT 
TTCATCAGTT TTTATATGCT CTGCGCATTT CTGATCATCA ACCTCTTCGT GGCTGTCATC 
ATGGACAATT TTGATTATCT CACCCGGGAC TGGTCTATTC TGGGGCCTCA CCATTTAGAT 
GAATTCAAAC GGATCTGGTC TGAATATGAC CCCGAGGCAA AGGGCAGGAT CAAGCACCTG 
GACGTGGTCA CTCTGCTCCG CCGCATCCAG CCTCCCCTGG GCTTCGGGAA GTTGTGTCCA 
CACCGGGTAG CCTGCAAGAG ACTAGTTGCT ATGAACATGC CACTCAACAG TGATGGGACA 
GTCATGTTTA ATGCAACTCT GTTTGCTCTG GTTCGAACGG CTCTTAAAAT CAAGACTGAA 
GGCAACCTGG AACAAGCTAA TGAAGAACTC CGAGCTGTGA TCAAGAAAAT TTGGAAGAAA 
ACCAGCATGA AACTACTTGA CCAAGTTGTT CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAACAAGGAC TGGTGGGAAA ATACCCCGCG AAGAACAGCA CAATTGCCCT ACAGGCGGGA 
TTAAGGACAC TGCATGACAT TGGGCCAGAA ATCCGGCGTG CTATATCATG CGATTTGCAA 
GATGATGATC CAGAGGAAAC AAAACGAGAA GACGAAGAAG ATGTGTTCAA AAGAAATGGT 
GCCCTGCTTG GAAACCATGT CAATCATGTT AATAGTGATA GGAGAGATTC CCTTCAGCAG 
ACCAATACCA CCCACCGTCC CCTGCATGTC CAAAGGCCTT CAATTCCACC TGCAAGTGAT 
ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA AATTCGGTGT GTCATAACCA TCATAACCAT 
AATTCCATAG GAAAGCAAGT TCCCAGCTCA ACAAATGCCA ATCTCAATAA TGCCAATATG 
TGCAAAGCTG CCCCTGGAAA GCGGCCCAGC ATTGGGAACC TTGAGCTTGT GTCTGAAAAC 
GGGCATCATT CCTTCCACAA GCATGGCCGT GAGCCTCACA GAAGGTGCAG TATTAAAAGA 
ACCCGCTATT ATGAAACTTA CATTAGGTCT GACTCAGGAG ACGAGCAGTT CCCGACGATT 
TGCCGGGAGG ACCCGGAGAT ACACGGCTAC TTCAGGGACC CCCACTGCCC AGGGGAGCAG 
GAGTATTTCA GCAGCGAGGA GTACTATGAG GATGACAGCT CGCCCACCGG GAGCAGGCAA 
AGCTACGGCT GCGGCTACTA TAACTGGTAC CTGGGCAGCA GCTTGGACTT TGAGTGGCCT 
CGAGGCTACC ATCACCCCCA AAGCTTCTCA GAGGACGACG ACTCGCCCGT TGGCTACGAT 
TCCCGGAGGT CTCCGAGGCG ACGCCTACTA CCTCCCACCC CTACATCCCC CCGCAGGTCT 
TCCTTCAACT TCGAGTGCCT GCGCCGGCAG AGCAGCCAGG AGGAGGTCCC GCCGTCGCCC 
GCCTTCCCGC CCCGCACGGC CCTGCCTCTG CACCTGATGC AGCAGCAGAT CATGGCAGTC 
GCCGGCCTAG ATTCCAGTAA AGCCCAGAAG TACTCGCCGA GCCACTCCAC CCGGTCGTGG 
GCCACGCCCC CGGCCACGCC GTCGTATCGG GACTGGACGC CGTGCTATAC CCCGCTGATC 
CAGGTTGAGC GGCCAGAGGC CCCAGACCAG GTCAACGGCA GCCTCCCGTC CCTGCACCGC 
AGCTCGTGGT ACACGGACGA GCCCAGCGTC TCCTATCGGA CGTTCACACC AGCCAGCCTG 
ACCGTCCCCA GCAGCTTCCA CAACAAGAAC AGCGACAAGC AGAGGAGCGC GGACAGCCTG 
GTGGAGGCAG TTCTGATATC CGAAGGCTTA GGACGGTACG CGAGGGACCC AAAGTTCGTG 
TCGGCGACAA AACACGAAAT CGCCGATGCC TGTGACTTAA CCATCGACGA GATGGAGAGT 
GCGGCCAGCA ACCTGCTGAA CGGGAACGTG CATCCGCGGG CCAATGGAGA CGTGGGCCCC 
GTTTCCCACC GGCAAGACTA CGAGCTCCAG GACTTTGGGC CTGGCTACAG CGACGAGGAG 
CCGGACCCCG GGAGGGACGA GGAGGACCTG GCAGACGAGA TGATTTGCAT CACCACCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815980.1
CDS	259..6681
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817678.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817678.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGATTGG TGAAGCTTCT CAGCAGGGGG GAAGGCATCC GGACGCTGCT GTGGACTTTT 
ATTAAGTCCT TCCAGGCGCT CCCGTATGTC GCCCTTCTTA TCGCCATGCT GTTCTTCATC 
TACGCGGTCA TCGGCATGCA GATGTTCGGG AAAGTGGCCA TGAGAGATAA CAACCAGATC 
AACAGAAACA ACAATTTCCA GACCTTTCCC CAGGCGGTGC TGCTGCTCTT CAGGTGTGCG 
ACGGGGGAGG CCTGGCAGGA AATCATGTTA GCCTGCCTCC CGGGGAAGCA ATGTGACCCC 
GACTCGGATT ACAACCCCGG GGAGGAATAC ACATGTGGGA GCAACTTCGC CATCGTTTAT 
TTCATCAGTT TTTATATGCT CTGCGCATTT CTGATCATCA ACCTCTTCGT GGCTGTCATC 
ATGGACAATT TTGATTATCT CACCCGGGAC TGGTCTATTC TGGGGCCTCA CCATTTAGAT 
GAATTCAAAC GGATCTGGTC TGAATATGAC CCCGAGGCAA AGGGCAGGAT CAAGCACCTG 
GACGTGGTCA CTCTGCTCCG CCGCATCCAG CCTCCCCTGG GCTTCGGGAA GTTGTGTCCA 
CACCGGGTAG CCTGCAAGAG ACTAGTTGCT ATGAACATGC CACTCAACAG TGATGGGACA 
GTCATGTTTA ATGCAACTCT GTTTGCTCTG GTTCGAACGG CTCTTAAAAT CAAGACTGAA 
GGCAACCTGG AACAAGCTAA TGAAGAACTC CGAGCTGTGA TCAAGAAAAT TTGGAAGAAA 
ACCAGCATGA AACTACTTGA CCAAGTTGTT CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAACAAGGAC TGGTGGGAAA ATACCCCGCG AAGAACAGCA CAATTGCCCT ACAGGCGGGA 
TTAAGGACAC TGCATGACAT TGGGCCAGAA ATCCGGCGTG CTATATCATG CGATTTGCAA 
GATGATGATC CAGAGGAAAC AAAACGAGAA GACGAAGAAG ATGTGTTCAA AAGAAATGGT 
GCCCTGCTTG GAAACCATGT CAATCATGTT AATAGTGATA GGAGAGATTC CCTTCAGCAG 
ACCAATACCA CCCACCGTCC CCTGCATGTC CAAAGGCCTT CAATTCCACC TGCAAGTGAT 
ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA AATTCGGTGT GTCATAACCA TCATAACCAT 
AATTCCATAG GAAAGCAAGT TCCCAGCTCA ACAAATGCCA ATCTCAATAA TGCCAATATG 
TGCAAAGCTG CCCCTGGAAA GCGGCCCAGC ATTGGGAACC TTGAGCTTGT GTCTGAAAAC 
GGGCATCATT CCTTCCACAA GCATGGCCGT GAGCCTCACA GAAGGTGCAG TATTAAAAGA 
ACCCGCTATT ATGAAACTTA CATTAGGTCT GACTCAGGAG ACGAGCAGTT CCCGACGATT 
TGCCGGGAGG ACCCGGAGAT ACACGGCTAC TTCAGGGACC CCCACTGCCC AGGGGAGCAG 
GAGTATTTCA GCAGCGAGGA GTACTATGAG GATGACAGCT CGCCCACCGG GAGCAGGCAA 
AGCTACGGCT GCGGCTACTA TAACTGGTAC CTGGGCAGCA GCTTGGACTT TGAGTGGCCT 
CGAGGCTACC ATCACCCCCA AAGCTTCTCA GAGGACGACG ACTCGCCCGT TGGCTACGAT 
TCCCGGAGGT CTCCGAGGCG ACGCCTACTA CCTCCCACCC CTACATCCCC CCGCAGGTCT 
TCCTTCAACT TCGAGTGCCT GCGCCGGCAG AGCAGCCAGG AGGAGGTCCC GCCGTCGCCC 
GCCTTCCCGC CCCGCACGGC CCTGCCTCTG CACCTGATGC AGCAGCAGAT CATGGCAGTC 
GCCGGCCTAG ATTCCAGTAA AGCCCAGAAG TACTCGCCGA GCCACTCCAC CCGGTCGTGG 
GCCACGCCCC CGGCCACGCC GTCGTATCGG GACTGGACGC CGTGCTATAC CCCGCTGATC 
CAGGTTGAGC GGCCAGAGGC CCCAGACCAG GTCAACGGCA GCCTCCCGTC CCTGCACCGC 
AGCTCGTGGT ACACGGACGA GCCCAGCGTC TCCTATCGGA CGTTCACACC AGCCAGCCTG 
ACCGTCCCCA GCAGCTTCCA CAACAAGAAC AGCGACAAGC AGAGGAGCGC GGACAGCCTG 
GTGGAGGCAG TTCTGATATC CGAAGGCTTA GGACGGTACG CGAGGGACCC AAAGTTCGTG 
TCGGCGACAA AACACGAAAT CGCCGATGCC TGTGACTTAA CCATCGACGA GATGGAGAGT 
GCGGCCAGCA ACCTGCTGAA CGGGAACGTG CATCCGCGGG CCAATGGAGA CGTGGGCCCC 
GTTTCCCACC GGCAAGACTA CGAGCTCCAG GACTTTGGGC CTGGCTACAG CGACGAGGAG 
CCGGACCCCG GGAGGGACGA GGAGGACCTG GCAGACGAGA TGATTTGCAT CACCACCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817679.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815981.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X18, mRNA.

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CloneID	OBa45776
Clone ID Related Accession (Same CDS sequence)	XM_010817679.1
Accession Version	XM_010817679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGATTGGTG 
AAGCTTCTCA GCAGGGGGGA AGGCATCCGG ACGCTGCTGT GGACTTTTAT TAAGTCCTTC 
CAGGCGCTCC CGTATGTCGC CCTTCTTATC GCCATGCTGT TCTTCATCTA CGCGGTCATC 
GGCATGCAGA TGTTCGGGAA AGTGGCCATG AGAGATAACA ACCAGATCAA CAGAAACAAC 
AATTTCCAGA CCTTTCCCCA GGCGGTGCTG CTGCTCTTCA GGTGTGCGAC GGGGGAGGCC 
TGGCAGGAAA TCATGTTAGC CTGCCTCCCG GGGAAGCAAT GTGACCCCGA CTCGGATTAC 
AACCCCGGGG AGGAATACAC ATGTGGGAGC AACTTCGCCA TCGTTTATTT CATCAGTTTT 
TATATGCTCT GCGCATTTCT GATCATCAAC CTCTTCGTGG CTGTCATCAT GGACAATTTT 
GATTATCTCA CCCGGGACTG GTCTATTCTG GGGCCTCACC ATTTAGATGA ATTCAAACGG 
ATCTGGTCTG AATATGACCC CGAGGCAAAG GGCAGGATCA AGCACCTGGA CGTGGTCACT 
CTGCTCCGCC GCATCCAGCC TCCCCTGGGC TTCGGGAAGT TGTGTCCACA CCGGGTAGCC 
TGCAAGAGAC TAGTTGCTAT GAACATGCCA CTCAACAGTG ATGGGACAGT CATGTTTAAT 
GCAACTCTGT TTGCTCTGGT TCGAACGGCT CTTAAAATCA AGACTGAAGG CAACCTGGAA 
CAAGCTAATG AAGAACTCCG AGCTGTGATC AAGAAAATTT GGAAGAAAAC CAGCATGAAA 
CTACTTGACC AAGTTGTTCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA ACAAGGACTG 
GTGGGAAAAT ACCCCGCGAA GAACAGCACA ATTGCCCTAC AGGCGGGATT AAGGACACTG 
CATGACATTG GGCCAGAAAT CCGGCGTGCT ATATCATGCG ATTTGCAAGA TGATGATCCA 
GAGGAAACAA AACGAGAAGA CGAAGAAGAT GTGTTCAAAA GAAATGGTGC CCTGCTTGGA 
AACCATGTCA ATCATGTTAA TAGTGATAGG AGAGATTCCC TTCAGCAGAC CAATACCACC 
CACCGTCCCC TGCATGTCCA AAGGCCTTCA ATTCCACCTG CAAGTGATAC TGAGAAACCG 
CTGTTTCCTC CAGCAGGAAA TTCGGTGTGT CATAACCATC ATAACCATAA TTCCATAGGA 
AAGCAAGTTC CCAGCTCAAC AAATGCCAAT CTCAATAATG CCAATATGTG CAAAGCTGCC 
CCTGGAAAGC GGCCCAGCAT TGGGAACCTT GAGCTTGTGT CTGAAAACGG GCATCATTCC 
TTCCACAAGC ATGGCCGTGA GCCTCACAGA AGGTGCAGTA TTAAAAGAAC CCGCTATTAT 
GAAACTTACA TTAGGTCTGA CTCAGGAGAC GAGCAGTTCC CGACGATTTG CCGGGAGGAC 
CCGGAGATAC ACGGCTACTT CAGGGACCCC CACTGCCCAG GGGAGCAGGA GTATTTCAGC 
AGCGAGGAGT ACTATGAGGA TGACAGCTCG CCCACCGGGA GCAGGCAAAG CTACGGCTGC 
GGCTACTATA ACTGGTACCT GGGCAGCAGC TTGGACTTTG AGTGGCCTCG AGGCTACCAT 
CACCCCCAAA GCTTCTCAGA GGACGACGAC TCGCCCGTTG GCTACGATTC CCGGAGGTCT 
CCGAGGCGAC GCCTACTACC TCCCACCCCT ACATCCCCCC GCAGGTCTTC CTTCAACTTC 
GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG GAGGTCCCGC CGTCGCCCGC CTTCCCGCCC 
CGCACGGCCC TGCCTCTGCA CCTGATGCAG CAGCAGATCA TGGCAGTCGC CGGCCTAGAT 
TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC CACTCCACCC GGTCGTGGGC CACGCCCCCG 
GCCACGCCGT CGTATCGGGA CTGGACGCCG TGCTATACCC CGCTGATCCA GGTTGAGCGG 
CCAGAGGCCC CAGACCAGGT CAACGGCAGC CTCCCGTCCC TGCACCGCAG CTCGTGGTAC 
ACGGACGAGC CCAGCGTCTC CTATCGGACG TTCACACCAG CCAGCCTGAC CGTCCCCAGC 
AGCTTCCACA ACAAGAACAG CGACAAGCAG AGGAGCGCGG ACAGCCTGGT GGAGGCAGTT 
CTGATATCCG AAGGCTTAGG ACGGTACGCG AGGGACCCAA AGTTCGTGTC GGCGACAAAA 
CACGAAATCG CCGATGCCTG TGACTTAACC ATCGACGAGA TGGAGAGTGC GGCCAGCAAC 
CTGCTGAACG GGAACGTGCA TCCGCGGGCC AATGGAGACG TGGGCCCCGT TTCCCACCGG 
CAAGACTACG AGCTCCAGGA CTTTGGGCCT GGCTACAGCG ACGAGGAGCC GGACCCCGGG 
AGGGACGAGG AGGACCTGGC AGACGAGATG ATTTGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815981.1
CDS	259..6789
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817679.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817679.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGATTGGTG 
AAGCTTCTCA GCAGGGGGGA AGGCATCCGG ACGCTGCTGT GGACTTTTAT TAAGTCCTTC 
CAGGCGCTCC CGTATGTCGC CCTTCTTATC GCCATGCTGT TCTTCATCTA CGCGGTCATC 
GGCATGCAGA TGTTCGGGAA AGTGGCCATG AGAGATAACA ACCAGATCAA CAGAAACAAC 
AATTTCCAGA CCTTTCCCCA GGCGGTGCTG CTGCTCTTCA GGTGTGCGAC GGGGGAGGCC 
TGGCAGGAAA TCATGTTAGC CTGCCTCCCG GGGAAGCAAT GTGACCCCGA CTCGGATTAC 
AACCCCGGGG AGGAATACAC ATGTGGGAGC AACTTCGCCA TCGTTTATTT CATCAGTTTT 
TATATGCTCT GCGCATTTCT GATCATCAAC CTCTTCGTGG CTGTCATCAT GGACAATTTT 
GATTATCTCA CCCGGGACTG GTCTATTCTG GGGCCTCACC ATTTAGATGA ATTCAAACGG 
ATCTGGTCTG AATATGACCC CGAGGCAAAG GGCAGGATCA AGCACCTGGA CGTGGTCACT 
CTGCTCCGCC GCATCCAGCC TCCCCTGGGC TTCGGGAAGT TGTGTCCACA CCGGGTAGCC 
TGCAAGAGAC TAGTTGCTAT GAACATGCCA CTCAACAGTG ATGGGACAGT CATGTTTAAT 
GCAACTCTGT TTGCTCTGGT TCGAACGGCT CTTAAAATCA AGACTGAAGG CAACCTGGAA 
CAAGCTAATG AAGAACTCCG AGCTGTGATC AAGAAAATTT GGAAGAAAAC CAGCATGAAA 
CTACTTGACC AAGTTGTTCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA ACAAGGACTG 
GTGGGAAAAT ACCCCGCGAA GAACAGCACA ATTGCCCTAC AGGCGGGATT AAGGACACTG 
CATGACATTG GGCCAGAAAT CCGGCGTGCT ATATCATGCG ATTTGCAAGA TGATGATCCA 
GAGGAAACAA AACGAGAAGA CGAAGAAGAT GTGTTCAAAA GAAATGGTGC CCTGCTTGGA 
AACCATGTCA ATCATGTTAA TAGTGATAGG AGAGATTCCC TTCAGCAGAC CAATACCACC 
CACCGTCCCC TGCATGTCCA AAGGCCTTCA ATTCCACCTG CAAGTGATAC TGAGAAACCG 
CTGTTTCCTC CAGCAGGAAA TTCGGTGTGT CATAACCATC ATAACCATAA TTCCATAGGA 
AAGCAAGTTC CCAGCTCAAC AAATGCCAAT CTCAATAATG CCAATATGTG CAAAGCTGCC 
CCTGGAAAGC GGCCCAGCAT TGGGAACCTT GAGCTTGTGT CTGAAAACGG GCATCATTCC 
TTCCACAAGC ATGGCCGTGA GCCTCACAGA AGGTGCAGTA TTAAAAGAAC CCGCTATTAT 
GAAACTTACA TTAGGTCTGA CTCAGGAGAC GAGCAGTTCC CGACGATTTG CCGGGAGGAC 
CCGGAGATAC ACGGCTACTT CAGGGACCCC CACTGCCCAG GGGAGCAGGA GTATTTCAGC 
AGCGAGGAGT ACTATGAGGA TGACAGCTCG CCCACCGGGA GCAGGCAAAG CTACGGCTGC 
GGCTACTATA ACTGGTACCT GGGCAGCAGC TTGGACTTTG AGTGGCCTCG AGGCTACCAT 
CACCCCCAAA GCTTCTCAGA GGACGACGAC TCGCCCGTTG GCTACGATTC CCGGAGGTCT 
CCGAGGCGAC GCCTACTACC TCCCACCCCT ACATCCCCCC GCAGGTCTTC CTTCAACTTC 
GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG GAGGTCCCGC CGTCGCCCGC CTTCCCGCCC 
CGCACGGCCC TGCCTCTGCA CCTGATGCAG CAGCAGATCA TGGCAGTCGC CGGCCTAGAT 
TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC CACTCCACCC GGTCGTGGGC CACGCCCCCG 
GCCACGCCGT CGTATCGGGA CTGGACGCCG TGCTATACCC CGCTGATCCA GGTTGAGCGG 
CCAGAGGCCC CAGACCAGGT CAACGGCAGC CTCCCGTCCC TGCACCGCAG CTCGTGGTAC 
ACGGACGAGC CCAGCGTCTC CTATCGGACG TTCACACCAG CCAGCCTGAC CGTCCCCAGC 
AGCTTCCACA ACAAGAACAG CGACAAGCAG AGGAGCGCGG ACAGCCTGGT GGAGGCAGTT 
CTGATATCCG AAGGCTTAGG ACGGTACGCG AGGGACCCAA AGTTCGTGTC GGCGACAAAA 
CACGAAATCG CCGATGCCTG TGACTTAACC ATCGACGAGA TGGAGAGTGC GGCCAGCAAC 
CTGCTGAACG GGAACGTGCA TCCGCGGGCC AATGGAGACG TGGGCCCCGT TTCCCACCGG 
CAAGACTACG AGCTCCAGGA CTTTGGGCCT GGCTACAGCG ACGAGGAGCC GGACCCCGGG 
AGGGACGAGG AGGACCTGGC AGACGAGATG ATTTGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817680.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815982.1 Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X9, mRNA.

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CloneID	OBa45777
Clone ID Related Accession (Same CDS sequence)	XM_010817680.2 , XM_010817680.1
Accession Version	XM_010817680.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817680.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815982.1
CDS	375..6821
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817680.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817680.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817680.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815982.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X19, mRNA.

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CloneID	OBa45777
Clone ID Related Accession (Same CDS sequence)	XM_010817680.2 , XM_010817680.1
Accession Version	XM_010817680.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815982.1
CDS	259..6705
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817680.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817680.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_010817681.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815983.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X20, mRNA.

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CloneID	OBa45778
Clone ID Related Accession (Same CDS sequence)	XM_010817681.1
Accession Version	XM_010817681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815983.1
CDS	259..6705
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_010817681.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817681.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGC TGCTGTGGAC TTTTATTAAG TCCTTCCAGG CGCTCCCGTA TGTCGCCCTT 
CTTATCGCCA TGCTGTTCTT CATCTACGCG GTCATCGGCA TGCAGATGTT CGGGAAAGTG 
GCCATGAGAG ATAACAACCA GATCAACAGA AACAACAATT TCCAGACCTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCGACGGGG GAGGCCTGGC AGGAAATCAT GTTAGCCTGC 
CTCCCGGGGA AGCAATGTGA CCCCGACTCG GATTACAACC CCGGGGAGGA ATACACATGT 
GGGAGCAACT TCGCCATCGT TTATTTCATC AGTTTTTATA TGCTCTGCGC ATTTCTGATC 
ATCAACCTCT TCGTGGCTGT CATCATGGAC AATTTTGATT ATCTCACCCG GGACTGGTCT 
ATTCTGGGGC CTCACCATTT AGATGAATTC AAACGGATCT GGTCTGAATA TGACCCCGAG 
GCAAAGGGCA GGATCAAGCA CCTGGACGTG GTCACTCTGC TCCGCCGCAT CCAGCCTCCC 
CTGGGCTTCG GGAAGTTGTG TCCACACCGG GTAGCCTGCA AGAGACTAGT TGCTATGAAC 
ATGCCACTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CTCTGTTTGC TCTGGTTCGA 
ACGGCTCTTA AAATCAAGAC TGAAGGCAAC CTGGAACAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATTTGGAA GAAAACCAGC ATGAAACTAC TTGACCAAGT TGTTCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC CGCGAAGAAC 
AGCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGCGATTT GCAAGATGAT GATCCAGAGG AAACAAAACG AGAAGACGAA 
GAAGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTGCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC TTGTGTCTGA AAACGGGCAT CATTCCTTCC ACAAGCATGG CCGTGAGCCT 
CACAGAAGGT GCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCTGACTCA 
GGAGACGAGC AGTTCCCGAC GATTTGCCGG GAGGACCCGG AGATACACGG CTACTTCAGG 
GACCCCCACT GCCCAGGGGA GCAGGAGTAT TTCAGCAGCG AGGAGTACTA TGAGGATGAC 
AGCTCGCCCA CCGGGAGCAG GCAAAGCTAC GGCTGCGGCT ACTATAACTG GTACCTGGGC 
AGCAGCTTGG ACTTTGAGTG GCCTCGAGGC TACCATCACC CCCAAAGCTT CTCAGAGGAC 
GACGACTCGC CCGTTGGCTA CGATTCCCGG AGGTCTCCGA GGCGACGCCT ACTACCTCCC 
ACCCCTACAT CCCCCCGCAG GTCTTCCTTC AACTTCGAGT GCCTGCGCCG GCAGAGCAGC 
CAGGAGGAGG TCCCGCCGTC GCCCGCCTTC CCGCCCCGCA CGGCCCTGCC TCTGCACCTG 
ATGCAGCAGC AGATCATGGC AGTCGCCGGC CTAGATTCCA GTAAAGCCCA GAAGTACTCG 
CCGAGCCACT CCACCCGGTC GTGGGCCACG CCCCCGGCCA CGCCGTCGTA TCGGGACTGG 
ACGCCGTGCT ATACCCCGCT GATCCAGGTT GAGCGGCCAG AGGCCCCAGA CCAGGTCAAC 
GGCAGCCTCC CGTCCCTGCA CCGCAGCTCG TGGTACACGG ACGAGCCCAG CGTCTCCTAT 
CGGACGTTCA CACCAGCCAG CCTGACCGTC CCCAGCAGCT TCCACAACAA GAACAGCGAC 
AAGCAGAGGA GCGCGGACAG CCTGGTGGAG GCAGTTCTGA TATCCGAAGG CTTAGGACGG 
TACGCGAGGG ACCCAAAGTT CGTGTCGGCG ACAAAACACG AAATCGCCGA TGCCTGTGAC 
TTAACCATCG ACGAGATGGA GAGTGCGGCC AGCAACCTGC TGAACGGGAA CGTGCATCCG 
CGGGCCAATG GAGACGTGGG CCCCGTTTCC CACCGGCAAG ACTACGAGCT CCAGGACTTT 
GGGCCTGGCT ACAGCGACGA GGAGCCGGAC CCCGGGAGGG ACGAGGAGGA CCTGGCAGAC 
GAGATGATTT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015459537.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315023.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X1, mRNA.

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CloneID	OBa94401
Clone ID Related Accession (Same CDS sequence)	XM_015459537.1
Accession Version	XM_015459537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA TTCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAATTCC ACCTGCAAGT GATACTGAGA AACCGCTGTT TCCTCCAGCA 
GGAAATTCGG TGTGTCATAA CCATCATAAC CATAATTCCA TAGGAAAGCA AGTTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTGCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCT TGTGTCTGAA AACGGGCATC ATTCCTTCCA CAAGCATGGC 
CGTGAGCCTC ACAGAAGGTG CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGG 
TCTGACTCAG GAGACGAGCA GTTCCCGACG ATTTGCCGGG AGGACCCGGA GATACACGGC 
TACTTCAGGG ACCCCCACTG CCCAGGGGAG CAGGAGTATT TCAGCAGCGA GGAGTACTAT 
GAGGATGACA GCTCGCCCAC CGGGAGCAGG CAAAGCTACG GCTGCGGCTA CTATAACTGG 
TACCTGGGCA GCAGCTTGGA CTTTGAGTGG CCTCGAGGCT ACCATCACCC CCAAAGCTTC 
TCAGAGGACG ACGACTCGCC CGTTGGCTAC GATTCCCGGA GGTCTCCGAG GCGACGCCTA 
CTACCTCCCA CCCCTACATC CCCCCGCAGG TCTTCCTTCA ACTTCGAGTG CCTGCGCCGG 
CAGAGCAGCC AGGAGGAGGT CCCGCCGTCG CCCGCCTTCC CGCCCCGCAC GGCCCTGCCT 
CTGCACCTGA TGCAGCAGCA GATCATGGCA GTCGCCGGCC TAGATTCCAG TAAAGCCCAG 
AAGTACTCGC CGAGCCACTC CACCCGGTCG TGGGCCACGC CCCCGGCCAC GCCGTCGTAT 
CGGGACTGGA CGCCGTGCTA TACCCCGCTG ATCCAGGTTG AGCGGCCAGA GGCCCCAGAC 
CAGGTCAACG GCAGCCTCCC GTCCCTGCAC CGCAGCTCGT GGTACACGGA CGAGCCCAGC 
GTCTCCTATC GGACGTTCAC ACCAGCCAGC CTGACCGTCC CCAGCAGCTT CCACAACAAG 
AACAGCGACA AGCAGAGGAG CGCGGACAGC CTGGTGGAGG CAGTTCTGAT ATCCGAAGGC 
TTAGGACGGT ACGCGAGGGA CCCAAAGTTC GTGTCGGCGA CAAAACACGA AATCGCCGAT 
GCCTGTGACT TAACCATCGA CGAGATGGAG AGTGCGGCCA GCAACCTGCT GAACGGGAAC 
GTGCATCCGC GGGCCAATGG AGACGTGGGC CCCGTTTCCC ACCGGCAAGA CTACGAGCTC 
CAGGACTTTG GGCCTGGCTA CAGCGACGAG GAGCCGGACC CCGGGAGGGA CGAGGAGGAC 
CTGGCAGACG AGATGATTTG CATCACCACC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315023.1
CDS	259..6894
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESESVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_015459537.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459537.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTCATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTCGGTG TCCTTAGTGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA TTCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAATTCC ACCTGCAAGT GATACTGAGA AACCGCTGTT TCCTCCAGCA 
GGAAATTCGG TGTGTCATAA CCATCATAAC CATAATTCCA TAGGAAAGCA AGTTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTGCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCT TGTGTCTGAA AACGGGCATC ATTCCTTCCA CAAGCATGGC 
CGTGAGCCTC ACAGAAGGTG CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGG 
TCTGACTCAG GAGACGAGCA GTTCCCGACG ATTTGCCGGG AGGACCCGGA GATACACGGC 
TACTTCAGGG ACCCCCACTG CCCAGGGGAG CAGGAGTATT TCAGCAGCGA GGAGTACTAT 
GAGGATGACA GCTCGCCCAC CGGGAGCAGG CAAAGCTACG GCTGCGGCTA CTATAACTGG 
TACCTGGGCA GCAGCTTGGA CTTTGAGTGG CCTCGAGGCT ACCATCACCC CCAAAGCTTC 
TCAGAGGACG ACGACTCGCC CGTTGGCTAC GATTCCCGGA GGTCTCCGAG GCGACGCCTA 
CTACCTCCCA CCCCTACATC CCCCCGCAGG TCTTCCTTCA ACTTCGAGTG CCTGCGCCGG 
CAGAGCAGCC AGGAGGAGGT CCCGCCGTCG CCCGCCTTCC CGCCCCGCAC GGCCCTGCCT 
CTGCACCTGA TGCAGCAGCA GATCATGGCA GTCGCCGGCC TAGATTCCAG TAAAGCCCAG 
AAGTACTCGC CGAGCCACTC CACCCGGTCG TGGGCCACGC CCCCGGCCAC GCCGTCGTAT 
CGGGACTGGA CGCCGTGCTA TACCCCGCTG ATCCAGGTTG AGCGGCCAGA GGCCCCAGAC 
CAGGTCAACG GCAGCCTCCC GTCCCTGCAC CGCAGCTCGT GGTACACGGA CGAGCCCAGC 
GTCTCCTATC GGACGTTCAC ACCAGCCAGC CTGACCGTCC CCAGCAGCTT CCACAACAAG 
AACAGCGACA AGCAGAGGAG CGCGGACAGC CTGGTGGAGG CAGTTCTGAT ATCCGAAGGC 
TTAGGACGGT ACGCGAGGGA CCCAAAGTTC GTGTCGGCGA CAAAACACGA AATCGCCGAT 
GCCTGTGACT TAACCATCGA CGAGATGGAG AGTGCGGCCA GCAACCTGCT GAACGGGAAC 
GTGCATCCGC GGGCCAATGG AGACGTGGGC CCCGTTTCCC ACCGGCAAGA CTACGAGCTC 
CAGGACTTTG GGCCTGGCTA CAGCGACGAG GAGCCGGACC CCGGGAGGGA CGAGGAGGAC 
CTGGCAGACG AGATGATTTG CATCACCACC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015459538.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315024.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X2, mRNA.

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CloneID	OBa94402
Clone ID Related Accession (Same CDS sequence)	XM_015459538.1
Accession Version	XM_015459538.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA TTCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAATTCC ACCTGCAAGT GATACTGAGA AACCGCTGTT TCCTCCAGCA 
GGAAATTCGG TGTGTCATAA CCATCATAAC CATAATTCCA TAGGAAAGCA AGTTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTGCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCT TGTGTCTGAA AACGGGCATC ATTCCTTCCA CAAGCATGGC 
CGTGAGCCTC ACAGAAGGTG CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGG 
TCTGACTCAG GAGACGAGCA GTTCCCGACG ATTTGCCGGG AGGACCCGGA GATACACGGC 
TACTTCAGGG ACCCCCACTG CCCAGGGGAG CAGGAGTATT TCAGCAGCGA GGAGTACTAT 
GAGGATGACA GCTCGCCCAC CGGGAGCAGG CAAAGCTACG GCTGCGGCTA CTATAACTGG 
TACCTGGGCA GCAGCTTGGA CTTTGAGTGG CCTCGAGGCT ACCATCACCC CCAAAGCTTC 
TCAGAGGACG ACGACTCGCC CGTTGGCTAC GATTCCCGGA GGTCTCCGAG GCGACGCCTA 
CTACCTCCCA CCCCTACATC CCCCCGCAGG TCTTCCTTCA ACTTCGAGTG CCTGCGCCGG 
CAGAGCAGCC AGGAGGAGGT CCCGCCGTCG CCCGCCTTCC CGCCCCGCAC GGCCCTGCCT 
CTGCACCTGA TGCAGCAGCA GATCATGGCA GTCGCCGGCC TAGATTCCAG TAAAGCCCAG 
AAGTACTCGC CGAGCCACTC CACCCGGTCG TGGGCCACGC CCCCGGCCAC GCCGTCGTAT 
CGGGACTGGA CGCCGTGCTA TACCCCGCTG ATCCAGGTTG AGCGGCCAGA GGCCCCAGAC 
CAGGTCAACG GCAGCCTCCC GTCCCTGCAC CGCAGCTCGT GGTACACGGA CGAGCCCAGC 
GTCTCCTATC GGACGTTCAC ACCAGCCAGC CTGACCGTCC CCAGCAGCTT CCACAACAAG 
AACAGCGACA AGCAGAGGAG CGCGGACAGC CTGGTGGAGG CAGTTCTGAT ATCCGAAGGC 
TTAGGACGGT ACGCGAGGGA CCCAAAGTTC GTGTCGGCGA CAAAACACGA AATCGCCGAT 
GCCTGTGACT TAACCATCGA CGAGATGGAG AGTGCGGCCA GCAACCTGCT GAACGGGAAC 
GTGCATCCGC GGGCCAATGG AGACGTGGGC CCCGTTTCCC ACCGGCAAGA CTACGAGCTC 
CAGGACTTTG GGCCTGGCTA CAGCGACGAG GAGCCGGACC CCGGGAGGGA CGAGGAGGAC 
CTGGCAGACG AGATGATTTG CATCACCACC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315024.1
CDS	259..6894
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESESVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_015459538.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459538.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGGT GCTGGTGGAG AAGAAGAGGC 
GCTGCCAAGG CCGGGCCCTC TGGGTGTCGG CGCTGGGGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA TTCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAATTCC ACCTGCAAGT GATACTGAGA AACCGCTGTT TCCTCCAGCA 
GGAAATTCGG TGTGTCATAA CCATCATAAC CATAATTCCA TAGGAAAGCA AGTTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTGCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCT TGTGTCTGAA AACGGGCATC ATTCCTTCCA CAAGCATGGC 
CGTGAGCCTC ACAGAAGGTG CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGG 
TCTGACTCAG GAGACGAGCA GTTCCCGACG ATTTGCCGGG AGGACCCGGA GATACACGGC 
TACTTCAGGG ACCCCCACTG CCCAGGGGAG CAGGAGTATT TCAGCAGCGA GGAGTACTAT 
GAGGATGACA GCTCGCCCAC CGGGAGCAGG CAAAGCTACG GCTGCGGCTA CTATAACTGG 
TACCTGGGCA GCAGCTTGGA CTTTGAGTGG CCTCGAGGCT ACCATCACCC CCAAAGCTTC 
TCAGAGGACG ACGACTCGCC CGTTGGCTAC GATTCCCGGA GGTCTCCGAG GCGACGCCTA 
CTACCTCCCA CCCCTACATC CCCCCGCAGG TCTTCCTTCA ACTTCGAGTG CCTGCGCCGG 
CAGAGCAGCC AGGAGGAGGT CCCGCCGTCG CCCGCCTTCC CGCCCCGCAC GGCCCTGCCT 
CTGCACCTGA TGCAGCAGCA GATCATGGCA GTCGCCGGCC TAGATTCCAG TAAAGCCCAG 
AAGTACTCGC CGAGCCACTC CACCCGGTCG TGGGCCACGC CCCCGGCCAC GCCGTCGTAT 
CGGGACTGGA CGCCGTGCTA TACCCCGCTG ATCCAGGTTG AGCGGCCAGA GGCCCCAGAC 
CAGGTCAACG GCAGCCTCCC GTCCCTGCAC CGCAGCTCGT GGTACACGGA CGAGCCCAGC 
GTCTCCTATC GGACGTTCAC ACCAGCCAGC CTGACCGTCC CCAGCAGCTT CCACAACAAG 
AACAGCGACA AGCAGAGGAG CGCGGACAGC CTGGTGGAGG CAGTTCTGAT ATCCGAAGGC 
TTAGGACGGT ACGCGAGGGA CCCAAAGTTC GTGTCGGCGA CAAAACACGA AATCGCCGAT 
GCCTGTGACT TAACCATCGA CGAGATGGAG AGTGCGGCCA GCAACCTGCT GAACGGGAAC 
GTGCATCCGC GGGCCAATGG AGACGTGGGC CCCGTTTCCC ACCGGCAAGA CTACGAGCTC 
CAGGACTTTG GGCCTGGCTA CAGCGACGAG GAGCCGGACC CCGGGAGGGA CGAGGAGGAC 
CTGGCAGACG AGATGATTTG CATCACCACC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015459539.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315025.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X7, mRNA.

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CloneID	OBa94403
Clone ID Related Accession (Same CDS sequence)	XM_015459539.1
Accession Version	XM_015459539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA TTCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAATTCC ACCTGCAAGT GATACTGAGA AACCGCTGTT TCCTCCAGCA 
GGAAATTCGG TGTGTCATAA CCATCATAAC CATAATTCCA TAGGAAAGCA AGTTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTGCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCT TGTGTCTGAA AACGGGCATC ATTCCTTCCA CAAGCATGGC 
CGTGAGCCTC ACAGAAGGTG CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGG 
TCTGACTCAG GAGACGAGCA GTTCCCGACG ATTTGCCGGG AGGACCCGGA GATACACGGC 
TACTTCAGGG ACCCCCACTG CCCAGGGGAG CAGGAGTATT TCAGCAGCGA GGAGTACTAT 
GAGGATGACA GCTCGCCCAC CGGGAGCAGG CAAAGCTACG GCTGCGGCTA CTATAACTGG 
TACCTGGGCA GCAGCTTGGA CTTTGAGTGG CCTCGAGGCT ACCATCACCC CCAAAGCTTC 
TCAGAGGACG ACGACTCGCC CGTTGGCTAC GATTCCCGGA GGTCTCCGAG GCGACGCCTA 
CTACCTCCCA CCCCTACATC CCCCCGCAGG TCTTCCTTCA ACTTCGAGTG CCTGCGCCGG 
CAGAGCAGCC AGGAGGAGGT CCCGCCGTCG CCCGCCTTCC CGCCCCGCAC GGCCCTGCCT 
CTGCACCTGA TGCAGCAGCA GATCATGGCA GTCGCCGGCC TAGATTCCAG TAAAGCCCAG 
AAGTACTCGC CGAGCCACTC CACCCGGTCG TGGGCCACGC CCCCGGCCAC GCCGTCGTAT 
CGGGACTGGA CGCCGTGCTA TACCCCGCTG ATCCAGGTTG AGCGGCCAGA GGCCCCAGAC 
CAGGTCAACG GCAGCCTCCC GTCCCTGCAC CGCAGCTCGT GGTACACGGA CGAGCCCAGC 
GTCTCCTATC GGACGTTCAC ACCAGCCAGC CTGACCGTCC CCAGCAGCTT CCACAACAAG 
AACAGCGACA AGCAGAGGAG CGCGGACAGC CTGGTGGAGG CAGTTCTGAT ATCCGAAGGC 
TTAGGACGGT ACGCGAGGGA CCCAAAGTTC GTGTCGGCGA CAAAACACGA AATCGCCGAT 
GCCTGTGACT TAACCATCGA CGAGATGGAG AGTGCGGCCA GCAACCTGCT GAACGGGAAC 
GTGCATCCGC GGGCCAATGG AGACGTGGGC CCCGTTTCCC ACCGGCAAGA CTACGAGCTC 
CAGGACTTTG GGCCTGGCTA CAGCGACGAG GAGCCGGACC CCGGGAGGGA CGAGGAGGAC 
CTGGCAGACG AGATGATTTG CATCACCACC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315025.1
CDS	259..6834
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDMVAEEDPAPCAFSGNGRQCTANGTECRSGWAGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFSRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMMTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPIYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIVDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESESVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKQCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNSTIALQAGLRTLHDIGPEIRRAISCDLQDDDPEETKREDEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPSSTNANLNNANMCKAAPGKRPSIGNLELVSENGHHSFHKHGREPHRRCSIKRTRYYETYIRSDSGDEQFPTICREDPEIHGYFRDPHCPGEQEYFSSEEYYEDDSSPTGSRQSYGCGYYNWYLGSSLDFEWPRGYHHPQSFSEDDDSPVGYDSRRSPRRRLLPPTPTSPRRSSFNFECLRRQSSQEEVPPSPAFPPRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPSYRDWTPCYTPLIQVERPEAPDQVNGSLPSLHRSSWYTDEPSVSYRTFTPASLTVPSSFHNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGNVHPRANGDVGPVSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_015459539.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459539.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGGCTCCCTC TATCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGACGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCCGCAC CCCCACCTGT CGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTGTCT CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAAACATT TTTGAAGATT 
ATAGCATATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTCATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAA TCAGGAGGCT TTGACGTCAA AGCCCTCCGC 
GCCTTTCGAG TGTTACGACC ACTTCGACTA GTATCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAGCTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATGGTGGCT GAAGAGGACC CAGCGCCGTG TGCGTTCTCA 
GGGAACGGAC GCCAGTGCAC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGCTGGCCCC 
AATGGCGGCA TCACCAACTT TGACAACTTT GCCTTTGCCA TGCTCACCGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGACGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAGTTCTCG AAGGAAAGGG AGAAAGCAAA AGCACGAGGA 
GACTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAAGAGG ATCTTAAGGG CTACTTGGAC 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAATGAAG AAGAAGGAGG CGAGGAGAGC 
AAACGAAATA CCAGCATGCC CACCAGTGAG ACGGAGTCTG TGAACACAGA GAACGTGAGC 
GGGGAAGGCG AGAACCGAGG CTGCTGTGGG AGTCTCTGTC AAGCCATCTC AAAGTCCAAG 
CTCAGCCGCC GCTGGCGCCG CTGGAACCGC TTCAGTCGCA GAAGGTGTAG GGCTGCTGTG 
AAGTCCGTCA CGTTTTACTG GCTGGTCATT GTCCTGGTGT TTCTCAACAC CTTAACCATT 
TCCTCCGAGC ACTACAACCA GCCTGACTGG TTGACACAGA TTCAAGATAT TGCTAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTCAAAA TGTACAGCTT GGGTCTCCAG 
GCGTACTTTG TCTCTCTTTT CAATCGGTTT GATTGCTTCG TGGTGTGCGG CGGAATTACC 
GAGACGATCC TGGTGGAACT GGAAATCATG TCTCCCCTGG GCATCTCTGT GTTCCGCTGT 
GTGCGGCTCT TGAGAATTTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGC TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTCGATAAT TTCCCGCAAG CCCTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGATTG GAATGCTGTG ATGTATGACG GTATCATGGC GTATGGGGGC 
CCATCATCTT CTGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGCGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTAGCTGA TGCTGAAAGT 
TTGAATACTG CTCAGAAGGA GGAAGCTGAA GAAAAGGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGTCTGG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CCATCGACGA CTACAGAGAA GAGGATGAGG ACAAGGACCC CTACCCACCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCTGAG TTGAACATGA AAGAGAAAAT CGCCCCCATC 
CCGGAAGGGA GTGCTTTCTT CATTCTCAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCCGCCCTCG CCGCTGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTGAA GATGATGACC 
TTTGGCGCTT TCCTCCACAA AGGCGCTTTC TGCAGGAACT ACTTCAACTT ACTGGATATG 
CTGGTTGTTG GGGTGTCTTT GGTGTCATTT GGCATCCAAT CCAGTGCCAT CTCTGTTGTG 
AAGATTCTGA GGGTCCTAAG GGTCCTGAGG CCTCTCAGGG CCATCAACAG AGCGAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CGATTGGCAA CATCATGATC 
GTCACCACGC TCCTCCAGTT CATGTTCGCC TGCATCGGGG TCCAGCTCTT CAAGGGAAAG 
TTCTATCGCT GCACAGACGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTCTTCATC 
CTCTACAAGG ATGGGGATGT TGATAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAACAGT 
GATTTCAACT TTGACAACGT CCTCTCGGCT ATGATGGCTC TGTTCACAGT CTCCACGTTC 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCC ATCGATTCGA ACGGAGAGAA TGTGGGCCCC 
ATCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTCGCA 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAGGCACGTC CGCTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTTA ATGACGCCAT GGACATCCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT TGCATTTAAG 
CCTAAGGGGT ACTTTAGTGA CGCCTGGAAC ACCTTTGACT CCCTCATCGT AATCGGCAGC 
ATTGTAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA GTGTCCCTGT CCCAACCGCT ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGAT TGGTGAAGCT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGCT GCTGTGGACT TTTATTAAGT CCTTCCAGGC GCTCCCGTAT 
GTCGCCCTTC TTATCGCCAT GCTGTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAAGTGG CCATGAGAGA TAACAACCAG ATCAACAGAA ACAACAATTT CCAGACCTTT 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGT GCGACGGGGG AGGCCTGGCA GGAAATCATG 
TTAGCCTGCC TCCCGGGGAA GCAATGTGAC CCCGACTCGG ATTACAACCC CGGGGAGGAA 
TACACATGTG GGAGCAACTT CGCCATCGTT TATTTCATCA GTTTTTATAT GCTCTGCGCA 
TTTCTGATCA TCAACCTCTT CGTGGCTGTC ATCATGGACA ATTTTGATTA TCTCACCCGG 
GACTGGTCTA TTCTGGGGCC TCACCATTTA GATGAATTCA AACGGATCTG GTCTGAATAT 
GACCCCGAGG CAAAGGGCAG GATCAAGCAC CTGGACGTGG TCACTCTGCT CCGCCGCATC 
CAGCCTCCCC TGGGCTTCGG GAAGTTGTGT CCACACCGGG TAGCCTGCAA GAGACTAGTT 
GCTATGAACA TGCCACTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC TCTGTTTGCT 
CTGGTTCGAA CGGCTCTTAA AATCAAGACT GAAGGCAACC TGGAACAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATTTGGAAG AAAACCAGCA TGAAACTACT TGACCAAGTT 
GTTCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCC 
GCGAAGAACA GCACAATTGC CCTACAGGCG GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGCGATTTG CAAGATGATG ATCCAGAGGA AACAAAACGA 
GAAGACGAAG AAGATGTGTT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA TTCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAATTCC ACCTGCAAGT GATACTGAGA AACCGCTGTT TCCTCCAGCA 
GGAAATTCGG TGTGTCATAA CCATCATAAC CATAATTCCA TAGGAAAGCA AGTTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTGCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCT TGTGTCTGAA AACGGGCATC ATTCCTTCCA CAAGCATGGC 
CGTGAGCCTC ACAGAAGGTG CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGG 
TCTGACTCAG GAGACGAGCA GTTCCCGACG ATTTGCCGGG AGGACCCGGA GATACACGGC 
TACTTCAGGG ACCCCCACTG CCCAGGGGAG CAGGAGTATT TCAGCAGCGA GGAGTACTAT 
GAGGATGACA GCTCGCCCAC CGGGAGCAGG CAAAGCTACG GCTGCGGCTA CTATAACTGG 
TACCTGGGCA GCAGCTTGGA CTTTGAGTGG CCTCGAGGCT ACCATCACCC CCAAAGCTTC 
TCAGAGGACG ACGACTCGCC CGTTGGCTAC GATTCCCGGA GGTCTCCGAG GCGACGCCTA 
CTACCTCCCA CCCCTACATC CCCCCGCAGG TCTTCCTTCA ACTTCGAGTG CCTGCGCCGG 
CAGAGCAGCC AGGAGGAGGT CCCGCCGTCG CCCGCCTTCC CGCCCCGCAC GGCCCTGCCT 
CTGCACCTGA TGCAGCAGCA GATCATGGCA GTCGCCGGCC TAGATTCCAG TAAAGCCCAG 
AAGTACTCGC CGAGCCACTC CACCCGGTCG TGGGCCACGC CCCCGGCCAC GCCGTCGTAT 
CGGGACTGGA CGCCGTGCTA TACCCCGCTG ATCCAGGTTG AGCGGCCAGA GGCCCCAGAC 
CAGGTCAACG GCAGCCTCCC GTCCCTGCAC CGCAGCTCGT GGTACACGGA CGAGCCCAGC 
GTCTCCTATC GGACGTTCAC ACCAGCCAGC CTGACCGTCC CCAGCAGCTT CCACAACAAG 
AACAGCGACA AGCAGAGGAG CGCGGACAGC CTGGTGGAGG CAGTTCTGAT ATCCGAAGGC 
TTAGGACGGT ACGCGAGGGA CCCAAAGTTC GTGTCGGCGA CAAAACACGA AATCGCCGAT 
GCCTGTGACT TAACCATCGA CGAGATGGAG AGTGCGGCCA GCAACCTGCT GAACGGGAAC 
GTGCATCCGC GGGCCAATGG AGACGTGGGC CCCGTTTCCC ACCGGCAAGA CTACGAGCTC 
CAGGACTTTG GGCCTGGCTA CAGCGACGAG GAGCCGGACC CCGGGAGGGA CGAGGAGGAC 
CTGGCAGACG AGATGATTTG CATCACCACC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Adrenal fasciculata cells express T-type and rapidly and slowly activating L-type Ca2+ channels that regulate cortisol secretion.
American journal of physiology. Cell physiology308(11)C899-918(2015 Jun)
Enyeart JJ,Enyeart JA

Expression profile of the pore-forming subunits ?1A and ?1D in the foetal bovine hypothalamus: a mammal with a long gestation.
Neuroscience letters556124-8(2013 Nov)
Peruffo A,Giacomello M,Montelli S,Panin M,Cozzi B

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Adrenal medulla calcium channel population is not conserved in bovine chromaffin cells in culture.
Neuroscience128(1)99-109(2004)
Benavides A,Calvo S,Tornero D,Gonz?lez-Garc?a C,Ce?a V

Differential expression of calcium channel subtypes in the bovine adrenal medulla.
Neuroendocrinology74(4)251-61(2001 Oct)
Garc?a-Palomero E,Renart J,Andr?s-Mateos E,Sol?s-Garrido LM,Matute C,Herrero CJ,Garc?a AG,Montiel C

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CACNA1D cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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