Cacna1d cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cacna1d
Entrez Gene ID	12289
Full Name	calcium channel, voltage-dependent, L type, alpha 1D subunit
Synonyms	8430418G19Rik,C79217,Cach3,Cacn4,Cacnl1a2,Cav1.3,Cchl1a,Cchl1a2,D-LTCC
General protein information	Preferred Names calcium channel, voltage-dependent, L type, alpha 1D subunit Names voltage-dependent L-type calcium channel subunit alpha-1D Cav1.3alpha1 alpha 1 E calcium channel, L type, alpha-1 polypeptide voltage-gated calcium channel subunit alpha Cav1.3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 18.43 cM\|14 B
Summary	This gene encodes a pore-forming subunit of the L-type, voltage-activated calcium channel family. These channels have been found to play a role in heart and smooth muscle contraction and in the transmission of auditory information. Homozygous knockout mice for this gene exhibit deafness and heart defects. These channels have also been linked to mitochondrial oxidative stress in a mouse model of Parkinson's disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017315808.1	XP_017171297.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
XM_017315809.1	XP_017171298.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
XM_017315811.1	XP_017171300.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
XM_017315816.1	XP_017171305.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
XM_017315818.1	XP_017171307.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
XM_006518480.1	XP_006518543.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
XM_017315821.1	XP_017171310.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X17
XM_017315824.1	XP_017171313.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X20
XM_017315808.2	XP_017171297.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
XM_017315809.2	XP_017171298.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
XM_017315811.2	XP_017171300.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
XM_017315813.2	XP_017171302.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
XM_017315815.2	XP_017171304.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
XM_017315817.2	XP_017171306.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X12
XM_017315810.1	XP_017171299.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
XM_017315812.1	XP_017171301.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
XM_017315815.1	XP_017171304.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
XM_006518479.1	XP_006518542.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
XM_017315820.1	XP_017171309.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
XM_017315825.1	XP_017171314.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X21
XM_006518475.2	XP_006518538.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
XM_017315812.2	XP_017171301.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
XM_017315814.2	XP_017171303.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
XM_017315816.2	XP_017171305.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
XM_017315822.2	XP_017171311.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X18
XM_017315814.1	XP_017171303.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
XM_017315817.1	XP_017171306.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X12
XM_017315819.1	XP_017171308.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
XM_017315823.1	XP_017171312.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X19
XM_017315819.2	XP_017171308.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
XM_006518480.2	XP_006518543.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
XM_017315824.2	XP_017171313.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X20
XM_017315825.2	XP_017171314.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X21
NM_001302637.1	NP_001289566.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform 3
NM_001083616.2	NP_001077085.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform 2
XM_017315813.1	XP_017171302.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
XM_017315810.2	XP_017171299.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
XM_006518479.2	XP_006518542.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
XM_017315821.2	XP_017171310.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X17
NM_028981.3	NP_083257.2	voltage-dependent L-type calcium channel subunit alpha-1D isoform 1
XM_006518475.1	XP_006518538.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
XM_017315822.1	XP_017171311.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X18
XM_017315818.2	XP_017171307.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
XM_017315820.2	XP_017171309.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
XM_017315823.2	XP_017171312.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X19

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CACNA1D	NP_001179954.1
Rattus norvegicus (Norway rat)	Cacna1d	NP_058994.1
Gallus gallus (chicken)	CACNA1D	NP_990365.1
Homo sapiens (human)	CACNA1D	NP_000711.1
Pan troglodytes (chimpanzee)	CACNA1D	XP_001173076.3
	CACNA1D	XP_002802833.1
Mus musculus (house mouse)	Cacna1d	NP_001077085.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012434	XP_001231059.2
Canis lupus familiaris (dog)	CACNA1D	XP_849431.1
Danio rerio (zebrafish)	cacna1da	NP_982351.1
Drosophila melanogaster (fruit fly)	Ca-alpha1D	NP_001260484.1
Caenorhabditis elegans (roundworm)	egl-19	NP_001023079.1

Gene Ontology
Bibliography

Related articles in PubMed

Cav1.3 and Cav1.2 channels of adrenal chromaffin cells: emerging views on cAMP/cGMP-mediated phosphorylation and role in pacemaking.
Vandael DH, Mahapatra S, Calorio C, Marcantoni A, Carbone E
Biochimica et biophysica acta1828(7)1608-18(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ca(2+) entry serves critical cellular functions in virtually every cell type, and appropriate regulation of Ca(2+) in neurons is essential for proper function.
Title: Inhibition of L-Type Ca

Auditory inner hair cells (IHCs) encode sounds into nerve impulses through fast and indefatigable Ca(2+)-dependent exocytosis at their ribbon synapses. We show that this synaptic process involves long and short C-terminal isoforms of the Cav1.3 Ca(2+) channel that differ in the kinetics of their Ca(2+)-dependent inactivation and their relative sensitivity to the l-type Ca(2+) channel blocker nifedipine.
Title: Different Ca

alpha1D Ca and SK4 channels are coupled in the atria, and deletion of alpha1D leads to decreased SK4 mRNA and BNP secretion providing evidence for a novel role of alpha1D in atrial endocrine function
Title: Novel function of ?

These results provide the evidence of a direct regulatory role of Snapin on Cav1.3 channels in atrial myocytes.
Title: Physical and functional interaction of Snapin with Cav1.3 calcium channel impacts channel protein trafficking in atrial myocytes.

Inner hair cells possess calcium channels that are essential for transmitting sound information into synaptic transmitter release. Voltage-gated calcium channels can coassemble with auxiliary subunit alpha2delta isoforms 1-4. We found that hair cells of the mouse express the auxiliary subunit alpha2delta2, which is needed for normal hearing thresholds. Using a mouse model with a mutant, nonfunctional alpha2delta2 prote...
Title: ?2?2 Controls the Function and Trans-Synaptic Coupling of Cav1.3 Channels in Mouse Inner Hair Cells and Is Essential for Normal Hearing.

The following Cacna1d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna1d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu78650	XM_017315820.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78651	XM_017315821.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78652	XM_017315822.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu49612	XM_006518475.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu49616	XM_006518479.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu49617	XM_006518480.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78638	XM_017315808.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78639	XM_017315809.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78640	XM_017315810.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78641	XM_017315811.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78642	XM_017315812.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78643	XM_017315813.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78644	XM_017315814.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78645	XM_017315815.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78646	XM_017315816.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78647	XM_017315817.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78648	XM_017315818.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78649	XM_017315819.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78653	XM_017315823.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78654	XM_017315824.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78655	XM_017315825.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu20903	NM_028981.3 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu20905	NM_001083616.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67259	NM_001302637.1 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu78638	XM_017315808.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78639	XM_017315809.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78641	XM_017315811.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78645	XM_017315815.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78642	XM_017315812.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78646	XM_017315816.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78644	XM_017315814.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78647	XM_017315817.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78649	XM_017315819.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu49617	XM_006518480.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu78654	XM_017315824.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78655	XM_017315825.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78643	XM_017315813.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78640	XM_017315810.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu49616	XM_006518479.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu78651	XM_017315821.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu49612	XM_006518475.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu78652	XM_017315822.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78648	XM_017315818.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu78650	XM_017315820.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78653	XM_017315823.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cacna1d ( XM_017315820.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171309.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X17, mRNA.

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CloneID	OMu78650
Clone ID Related Accession (Same CDS sequence)	XM_017315820.1 , XM_017315820.2
Accession Version	XM_017315820.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGGAAA TTCAAGAAAC GGAAAGAGCA AGGACTGGTG GGGAAGTACC 
CTGCGAAGAA CACCACGATT GCCCTACAGA TGCTTGAACG GATGCTTTAG AGTTCTCTGC 
CTGAGCTACC GCACCAAGCT GGTTAGTCCG AAGGCGCTTG TGGCTAAGGC CTTGAAAGGG 
CAAACTCCCA CTCGGGCTTA TTTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171309.1
CDS	561..5690
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGGNSRNGKSKDWWGSTLRRTPRLPYRCLNGCFRVLCLSYRTKLVSPKALVAKALKGQTPTRAYFC

Target ORF information:

RefSeq Version	XM_017315820.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315820.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGGAAA TTCAAGAAAC GGAAAGAGCA AGGACTGGTG GGGAAGTACC 
CTGCGAAGAA CACCACGATT GCCCTACAGA TGCTTGAACG GATGCTTTAG AGTTCTCTGC 
CTGAGCTACC GCACCAAGCT GGTTAGTCCG AAGGCGCTTG TGGCTAAGGC CTTGAAAGGG 
CAAACTCCCA CTCGGGCTTA TTTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315820.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171309.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X17, mRNA.

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CloneID	OMu78650
Clone ID Related Accession (Same CDS sequence)	XM_017315820.1 , XM_017315820.2
Accession Version	XM_017315820.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315820.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGGAAA TTCAAGAAAC GGAAAGAGCA AGGACTGGTG GGGAAGTACC 
CTGCGAAGAA CACCACGATT GCCCTACAGA TGCTTGAACG GATGCTTTAG AGTTCTCTGC 
CTGAGCTACC GCACCAAGCT GGTTAGTCCG AAGGCGCTTG TGGCTAAGGC CTTGAAAGGG 
CAAACTCCCA CTCGGGCTTA TTTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171309.1
CDS	494..5623
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGGNSRNGKSKDWWGSTLRRTPRLPYRCLNGCFRVLCLSYRTKLVSPKALVAKALKGQTPTRAYFC

Target ORF information:

RefSeq Version	XM_017315820.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315820.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGGAAA TTCAAGAAAC GGAAAGAGCA AGGACTGGTG GGGAAGTACC 
CTGCGAAGAA CACCACGATT GCCCTACAGA TGCTTGAACG GATGCTTTAG AGTTCTCTGC 
CTGAGCTACC GCACCAAGCT GGTTAGTCCG AAGGCGCTTG TGGCTAAGGC CTTGAAAGGG 
CAAACTCCCA CTCGGGCTTA TTTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315821.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171310.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X18, mRNA.

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CloneID	OMu78651
Clone ID Related Accession (Same CDS sequence)	XM_017315821.1 , XM_017315821.2
Accession Version	XM_017315821.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGATG CTTGAACGGA TGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171310.1
CDS	561..5648
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQMLERML

Target ORF information:

RefSeq Version	XM_017315821.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315821.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGATG CTTGAACGGA TGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315821.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171310.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X18, mRNA.

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CloneID	OMu78651
Clone ID Related Accession (Same CDS sequence)	XM_017315821.1 , XM_017315821.2
Accession Version	XM_017315821.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315821.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGATG CTTGAACGGA TGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171310.1
CDS	494..5581
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQMLERML

Target ORF information:

RefSeq Version	XM_017315821.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315821.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGATG CTTGAACGGA TGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315822.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171311.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X19, mRNA.

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CloneID	OMu78652
Clone ID Related Accession (Same CDS sequence)	XM_017315822.2 , XM_017315822.1
Accession Version	XM_017315822.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315822.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGGAAA TTCAAGAAAC GGAAAGAGCA AGGACTGGTG GGGAAGTACC 
CTGCGAAGAA CACCACGATT GCCCTACAGG CGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171311.1
CDS	494..5512
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGGNSRNGKSKDWWGSTLRRTPRLPYRRD

Target ORF information:

RefSeq Version	XM_017315822.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315822.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGGAAA TTCAAGAAAC GGAAAGAGCA AGGACTGGTG GGGAAGTACC 
CTGCGAAGAA CACCACGATT GCCCTACAGG CGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315822.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171311.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X19, mRNA.

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CloneID	OMu78652
Clone ID Related Accession (Same CDS sequence)	XM_017315822.2 , XM_017315822.1
Accession Version	XM_017315822.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGGAAA TTCAAGAAAC GGAAAGAGCA AGGACTGGTG GGGAAGTACC 
CTGCGAAGAA CACCACGATT GCCCTACAGG CGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171311.1
CDS	561..5579
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGGNSRNGKSKDWWGSTLRRTPRLPYRRD

Target ORF information:

RefSeq Version	XM_017315822.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315822.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGGAAA TTCAAGAAAC GGAAAGAGCA AGGACTGGTG GGGAAGTACC 
CTGCGAAGAA CACCACGATT GCCCTACAGG CGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_006518475.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518538.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X1, mRNA.

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CloneID	OMu49612
Clone ID Related Accession (Same CDS sequence)	XM_006518475.2 , XM_006518475.1
Accession Version	XM_006518475.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006518475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518538.1
CDS	494..7117
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_006518475.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518475.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_006518479.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518542.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X11, mRNA.

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CloneID	OMu49616
Clone ID Related Accession (Same CDS sequence)	XM_006518479.1 , XM_006518479.2
Accession Version	XM_006518479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006518479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518542.1
CDS	494..6973
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_006518479.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518479.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_006518480.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518543.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X15, mRNA.

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CloneID	OMu49617
Clone ID Related Accession (Same CDS sequence)	XM_006518480.1 , XM_006518480.2
Accession Version	XM_006518480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006518480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGGTC TGAGTCAGGA GATGAGCAAT TTCCAACCAT TTGCCGTGAA 
GATCCTGAGA TACACGGCTA CTTCCGAGAC CCCCGCTGCT TGGGGGAACA GGAGTATTTC 
AGTAGCGAGG AGTGCTGTGA GGACGACAGC TCTCCCACCT GGAGCAGGCA AAACTACAAC 
TACTACAACA GGTACCCAGG CAGCTCCATG GACTTTGAGA GGCCCCGAGG CTACCACCAC 
CCCCAAGGCT TCTTAGAAGA TGATGACTCT CCCACTGGCT ATGATTCACG GAGATCTCCG 
AGGAGACGTC TTCTACCTCC TACCCCACCA TCCCATCGGA GGTCCTCCTT CAACTTTGAG 
TGTCTGCGCA GGCAAAGCAG CCAAGACGAT GTCCTTCCAT CGCCTGCCCT GCCTCACCGC 
GCTGCCCTGC CCCTGCACCT GATGCAGCAG CAGATCATGG CCGTTGCAGG CCTAGATTCC 
AGTAAAGCCC AGAAGTACTC ACCGAGCCAC TCTACCCGGT CGTGGGCCAC CCCTCCAGCA 
ACCCCTCCAT ACCGAGACTG GAGCCCATGC TACACCCCTT TGATCCAGGT GGACCGCTCA 
GAATCTATGG ACCAGGTCAA TGGCAGCCTG CCATCCCTAC ACCGAAGCTC CTGGTACACA 
GATGAACCCG ACATCTCCTA TAGGACTTTC ACGCCAGCTA GTCTGACTGT CCCCAGCAGC 
TTCCGGAACA AGAACAGCGA CAAGCAGAGG AGTGCAGACA GCCTGGTGGA GGCAGTCCTG 
ATATCCGAAG GCTTGGGACG CTACGCGAGG GACCCCAAAT TTGTCTCAGC GACAAAACAT 
GAGATTGCCG ATGCCTGTGA CCTAACCATA GACGAGATGG AAAGTGCTGC CAGCACCCTG 
CTCAATGGCA GTGTGTGTCC TCGAGCCAAT GGGGACATGG GCCCCATCTC CCACCGCCAG 
GACTATGAGC TCCAGGACTT TGGTCCTGGC TACAGTGACG AGGAGCCAGA CCCTGGGCGA 
GAGGAAGAGG ACCTGGCAGA TGAGATGATT TGCATTACCA CCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518543.1
CDS	494..6901
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_006518480.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518480.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGGTC TGAGTCAGGA GATGAGCAAT TTCCAACCAT TTGCCGTGAA 
GATCCTGAGA TACACGGCTA CTTCCGAGAC CCCCGCTGCT TGGGGGAACA GGAGTATTTC 
AGTAGCGAGG AGTGCTGTGA GGACGACAGC TCTCCCACCT GGAGCAGGCA AAACTACAAC 
TACTACAACA GGTACCCAGG CAGCTCCATG GACTTTGAGA GGCCCCGAGG CTACCACCAC 
CCCCAAGGCT TCTTAGAAGA TGATGACTCT CCCACTGGCT ATGATTCACG GAGATCTCCG 
AGGAGACGTC TTCTACCTCC TACCCCACCA TCCCATCGGA GGTCCTCCTT CAACTTTGAG 
TGTCTGCGCA GGCAAAGCAG CCAAGACGAT GTCCTTCCAT CGCCTGCCCT GCCTCACCGC 
GCTGCCCTGC CCCTGCACCT GATGCAGCAG CAGATCATGG CCGTTGCAGG CCTAGATTCC 
AGTAAAGCCC AGAAGTACTC ACCGAGCCAC TCTACCCGGT CGTGGGCCAC CCCTCCAGCA 
ACCCCTCCAT ACCGAGACTG GAGCCCATGC TACACCCCTT TGATCCAGGT GGACCGCTCA 
GAATCTATGG ACCAGGTCAA TGGCAGCCTG CCATCCCTAC ACCGAAGCTC CTGGTACACA 
GATGAACCCG ACATCTCCTA TAGGACTTTC ACGCCAGCTA GTCTGACTGT CCCCAGCAGC 
TTCCGGAACA AGAACAGCGA CAAGCAGAGG AGTGCAGACA GCCTGGTGGA GGCAGTCCTG 
ATATCCGAAG GCTTGGGACG CTACGCGAGG GACCCCAAAT TTGTCTCAGC GACAAAACAT 
GAGATTGCCG ATGCCTGTGA CCTAACCATA GACGAGATGG AAAGTGCTGC CAGCACCCTG 
CTCAATGGCA GTGTGTGTCC TCGAGCCAAT GGGGACATGG GCCCCATCTC CCACCGCCAG 
GACTATGAGC TCCAGGACTT TGGTCCTGGC TACAGTGACG AGGAGCCAGA CCCTGGGCGA 
GAGGAAGAGG ACCTGGCAGA TGAGATGATT TGCATTACCA CCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315808.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171297.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X2, mRNA.

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CloneID	OMu78638
Clone ID Related Accession (Same CDS sequence)	XM_017315808.1 , XM_017315808.2
Accession Version	XM_017315808.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171297.1
CDS	561..7184
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315808.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315808.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315808.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171297.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X2, mRNA.

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CloneID	OMu78638
Clone ID Related Accession (Same CDS sequence)	XM_017315808.1 , XM_017315808.2
Accession Version	XM_017315808.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315808.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171297.1
CDS	494..7117
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315808.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315808.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315809.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171298.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X3, mRNA.

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CloneID	OMu78639
Clone ID Related Accession (Same CDS sequence)	XM_017315809.1 , XM_017315809.2
Accession Version	XM_017315809.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGGTCT GAGTCAGGAG ATGAGCAATT TCCAACCATT 
TGCCGTGAAG ATCCTGAGAT ACACGGCTAC TTCCGAGACC CCCGCTGCTT GGGGGAACAG 
GAGTATTTCA GTAGCGAGGA GTGCTGTGAG GACGACAGCT CTCCCACCTG GAGCAGGCAA 
AACTACAACT ACTACAACAG GTACCCAGGC AGCTCCATGG ACTTTGAGAG GCCCCGAGGC 
TACCACCACC CCCAAGGCTT CTTAGAAGAT GATGACTCTC CCACTGGCTA TGATTCACGG 
AGATCTCCGA GGAGACGTCT TCTACCTCCT ACCCCACCAT CCCATCGGAG GTCCTCCTTC 
AACTTTGAGT GTCTGCGCAG GCAAAGCAGC CAAGACGATG TCCTTCCATC GCCTGCCCTG 
CCTCACCGCG CTGCCCTGCC CCTGCACCTG ATGCAGCAGC AGATCATGGC CGTTGCAGGC 
CTAGATTCCA GTAAAGCCCA GAAGTACTCA CCGAGCCACT CTACCCGGTC GTGGGCCACC 
CCTCCAGCAA CCCCTCCATA CCGAGACTGG AGCCCATGCT ACACCCCTTT GATCCAGGTG 
GACCGCTCAG AATCTATGGA CCAGGTCAAT GGCAGCCTGC CATCCCTACA CCGAAGCTCC 
TGGTACACAG ATGAACCCGA CATCTCCTAT AGGACTTTCA CGCCAGCTAG TCTGACTGTC 
CCCAGCAGCT TCCGGAACAA GAACAGCGAC AAGCAGAGGA GTGCAGACAG CCTGGTGGAG 
GCAGTCCTGA TATCCGAAGG CTTGGGACGC TACGCGAGGG ACCCCAAATT TGTCTCAGCG 
ACAAAACATG AGATTGCCGA TGCCTGTGAC CTAACCATAG ACGAGATGGA AAGTGCTGCC 
AGCACCCTGC TCAATGGCAG TGTGTGTCCT CGAGCCAATG GGGACATGGG CCCCATCTCC 
CACCGCCAGG ACTATGAGCT CCAGGACTTT GGTCCTGGCT ACAGTGACGA GGAGCCAGAC 
CCTGGGCGAG AGGAAGAGGA CCTGGCAGAT GAGATGATTT GCATTACCAC CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171298.1
CDS	561..7157
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315809.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315809.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGGTCT GAGTCAGGAG ATGAGCAATT TCCAACCATT 
TGCCGTGAAG ATCCTGAGAT ACACGGCTAC TTCCGAGACC CCCGCTGCTT GGGGGAACAG 
GAGTATTTCA GTAGCGAGGA GTGCTGTGAG GACGACAGCT CTCCCACCTG GAGCAGGCAA 
AACTACAACT ACTACAACAG GTACCCAGGC AGCTCCATGG ACTTTGAGAG GCCCCGAGGC 
TACCACCACC CCCAAGGCTT CTTAGAAGAT GATGACTCTC CCACTGGCTA TGATTCACGG 
AGATCTCCGA GGAGACGTCT TCTACCTCCT ACCCCACCAT CCCATCGGAG GTCCTCCTTC 
AACTTTGAGT GTCTGCGCAG GCAAAGCAGC CAAGACGATG TCCTTCCATC GCCTGCCCTG 
CCTCACCGCG CTGCCCTGCC CCTGCACCTG ATGCAGCAGC AGATCATGGC CGTTGCAGGC 
CTAGATTCCA GTAAAGCCCA GAAGTACTCA CCGAGCCACT CTACCCGGTC GTGGGCCACC 
CCTCCAGCAA CCCCTCCATA CCGAGACTGG AGCCCATGCT ACACCCCTTT GATCCAGGTG 
GACCGCTCAG AATCTATGGA CCAGGTCAAT GGCAGCCTGC CATCCCTACA CCGAAGCTCC 
TGGTACACAG ATGAACCCGA CATCTCCTAT AGGACTTTCA CGCCAGCTAG TCTGACTGTC 
CCCAGCAGCT TCCGGAACAA GAACAGCGAC AAGCAGAGGA GTGCAGACAG CCTGGTGGAG 
GCAGTCCTGA TATCCGAAGG CTTGGGACGC TACGCGAGGG ACCCCAAATT TGTCTCAGCG 
ACAAAACATG AGATTGCCGA TGCCTGTGAC CTAACCATAG ACGAGATGGA AAGTGCTGCC 
AGCACCCTGC TCAATGGCAG TGTGTGTCCT CGAGCCAATG GGGACATGGG CCCCATCTCC 
CACCGCCAGG ACTATGAGCT CCAGGACTTT GGTCCTGGCT ACAGTGACGA GGAGCCAGAC 
CCTGGGCGAG AGGAAGAGGA CCTGGCAGAT GAGATGATTT GCATTACCAC CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315809.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171298.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X3, mRNA.

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CloneID	OMu78639
Clone ID Related Accession (Same CDS sequence)	XM_017315809.1 , XM_017315809.2
Accession Version	XM_017315809.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGGTCT GAGTCAGGAG ATGAGCAATT TCCAACCATT 
TGCCGTGAAG ATCCTGAGAT ACACGGCTAC TTCCGAGACC CCCGCTGCTT GGGGGAACAG 
GAGTATTTCA GTAGCGAGGA GTGCTGTGAG GACGACAGCT CTCCCACCTG GAGCAGGCAA 
AACTACAACT ACTACAACAG GTACCCAGGC AGCTCCATGG ACTTTGAGAG GCCCCGAGGC 
TACCACCACC CCCAAGGCTT CTTAGAAGAT GATGACTCTC CCACTGGCTA TGATTCACGG 
AGATCTCCGA GGAGACGTCT TCTACCTCCT ACCCCACCAT CCCATCGGAG GTCCTCCTTC 
AACTTTGAGT GTCTGCGCAG GCAAAGCAGC CAAGACGATG TCCTTCCATC GCCTGCCCTG 
CCTCACCGCG CTGCCCTGCC CCTGCACCTG ATGCAGCAGC AGATCATGGC CGTTGCAGGC 
CTAGATTCCA GTAAAGCCCA GAAGTACTCA CCGAGCCACT CTACCCGGTC GTGGGCCACC 
CCTCCAGCAA CCCCTCCATA CCGAGACTGG AGCCCATGCT ACACCCCTTT GATCCAGGTG 
GACCGCTCAG AATCTATGGA CCAGGTCAAT GGCAGCCTGC CATCCCTACA CCGAAGCTCC 
TGGTACACAG ATGAACCCGA CATCTCCTAT AGGACTTTCA CGCCAGCTAG TCTGACTGTC 
CCCAGCAGCT TCCGGAACAA GAACAGCGAC AAGCAGAGGA GTGCAGACAG CCTGGTGGAG 
GCAGTCCTGA TATCCGAAGG CTTGGGACGC TACGCGAGGG ACCCCAAATT TGTCTCAGCG 
ACAAAACATG AGATTGCCGA TGCCTGTGAC CTAACCATAG ACGAGATGGA AAGTGCTGCC 
AGCACCCTGC TCAATGGCAG TGTGTGTCCT CGAGCCAATG GGGACATGGG CCCCATCTCC 
CACCGCCAGG ACTATGAGCT CCAGGACTTT GGTCCTGGCT ACAGTGACGA GGAGCCAGAC 
CCTGGGCGAG AGGAAGAGGA CCTGGCAGAT GAGATGATTT GCATTACCAC CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171298.1
CDS	494..7090
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315809.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315809.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGGTCT GAGTCAGGAG ATGAGCAATT TCCAACCATT 
TGCCGTGAAG ATCCTGAGAT ACACGGCTAC TTCCGAGACC CCCGCTGCTT GGGGGAACAG 
GAGTATTTCA GTAGCGAGGA GTGCTGTGAG GACGACAGCT CTCCCACCTG GAGCAGGCAA 
AACTACAACT ACTACAACAG GTACCCAGGC AGCTCCATGG ACTTTGAGAG GCCCCGAGGC 
TACCACCACC CCCAAGGCTT CTTAGAAGAT GATGACTCTC CCACTGGCTA TGATTCACGG 
AGATCTCCGA GGAGACGTCT TCTACCTCCT ACCCCACCAT CCCATCGGAG GTCCTCCTTC 
AACTTTGAGT GTCTGCGCAG GCAAAGCAGC CAAGACGATG TCCTTCCATC GCCTGCCCTG 
CCTCACCGCG CTGCCCTGCC CCTGCACCTG ATGCAGCAGC AGATCATGGC CGTTGCAGGC 
CTAGATTCCA GTAAAGCCCA GAAGTACTCA CCGAGCCACT CTACCCGGTC GTGGGCCACC 
CCTCCAGCAA CCCCTCCATA CCGAGACTGG AGCCCATGCT ACACCCCTTT GATCCAGGTG 
GACCGCTCAG AATCTATGGA CCAGGTCAAT GGCAGCCTGC CATCCCTACA CCGAAGCTCC 
TGGTACACAG ATGAACCCGA CATCTCCTAT AGGACTTTCA CGCCAGCTAG TCTGACTGTC 
CCCAGCAGCT TCCGGAACAA GAACAGCGAC AAGCAGAGGA GTGCAGACAG CCTGGTGGAG 
GCAGTCCTGA TATCCGAAGG CTTGGGACGC TACGCGAGGG ACCCCAAATT TGTCTCAGCG 
ACAAAACATG AGATTGCCGA TGCCTGTGAC CTAACCATAG ACGAGATGGA AAGTGCTGCC 
AGCACCCTGC TCAATGGCAG TGTGTGTCCT CGAGCCAATG GGGACATGGG CCCCATCTCC 
CACCGCCAGG ACTATGAGCT CCAGGACTTT GGTCCTGGCT ACAGTGACGA GGAGCCAGAC 
CCTGGGCGAG AGGAAGAGGA CCTGGCAGAT GAGATGATTT GCATTACCAC CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315810.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171299.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X4, mRNA.

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CloneID	OMu78640
Clone ID Related Accession (Same CDS sequence)	XM_017315810.1 , XM_017315810.2
Accession Version	XM_017315810.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAACTTCTCA GCAGGGGGGA AGGCATCCGG ACACTGCTGT GGACCTTCAT CAAGTCCTTT 
CAGGCGCTGC CGTATGTTGC CCTCCTCATT GCCATGCTCT TCTTCATCTA CGCTGTCATA 
GGCATGCAGA TGTTTGGGAA AGTTGCCATG AGAGATAACA ACCAGATCAA TAGGAACAAC 
AACTTCCAGA CCTTTCCTCA GGCAGTGCTC CTACTCTTCA GGTGTGCAAC AGGGGAAGCC 
TGGCAGGAGA TCATGCTTGC CTGCCTCCCA GGAAAGCTGT GCGACCCAGA CTCCGATTAC 
AACCCAGGAG AGGAGTACAC TTGTGGGAGC AACTTTGCCA TTGTCTACTT CATCAGCTTT 
TACATGCTCT GTGCATTCCT GATCATCAAC CTCTTTGTGG CTGTCATCAT GGACAATTTT 
GACTATCTGA CTCGGGACTG GTCTATTCTG GGGCCTCACC ACTTGGACGA ATTCAAAAGA 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AACACCTTGA TGTGGTCACT 
CTGCTCCGGC GGATCCAGCC TCCCCTGGGG TTTGGAAAAT TATGCCCACA CCGAGTAGCA 
TGTAAGAGAT TGGTTGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTCAAT 
GCAACCCTGT TTGCTTTGGT CCGGACAGCT CTCAAGATCA AGACTGAAGG GAACCTGGAG 
CAAGCTAATG AAGAACTCCG AGCTGTGATC AAGAAAATCT GGAAGAAGAC AAGCATGAAG 
CTGCTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA GCAAGGACTG 
GTGGGGAAGT ACCCTGCGAA GAACACCACG ATTGCCCTAC AGGCGGGATT AAGGACCCTG 
CATGACATTG GGCCAGAAAT CCGACGGGCT ATATCCTGTG ACTTGCAAGA TGACGAGCCA 
GAAGACTCCA AACCAGAAGA AGAAGATGTA TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC 
CATGTCAATC ATGTTAATAG TGATAGGAGA GATTCCCTTC AGCAGACCAA TACCACCCAC 
CGTCCCCTGC ATGTCCAAAG GCCTTCAATG CCACCTGCAA GTGATACTGA GAAACCGCTG 
TTTCCTCCAG CAGGAAATTC GGGGTGTCAT AACCATCATA ACCATAATTC CATAGGGAAG 
CAAGCTCCCA CCTCAACAAA TGCCAATCTC AATAATGCCA ATATGTCCAA AGCTGCCCAC 
GGAAAGCCGC CCAGCATTGG GAACCTTGAG CATGTGTCTG AAAATGGGCA TTACTCCTGC 
AAGCATGATC GAGAGCTTCA AAGAAGGTCC AGTATTAAAA GAACCCGCTA TTACGAGACT 
TACATTAGGT CTGAGTCAGG AGATGAGCAA TTTCCAACCA TTTGCCGTGA AGATCCTGAG 
ATACACGGCT ACTTCCGAGA CCCCCGCTGC TTGGGGGAAC AGGAGTATTT CAGTAGCGAG 
GAGTGCTGTG AGGACGACAG CTCTCCCACC TGGAGCAGGC AAAACTACAA CTACTACAAC 
AGGTACCCAG GCAGCTCCAT GGACTTTGAG AGGCCCCGAG GCTACCACCA CCCCCAAGGC 
TTCTTAGAAG ATGATGACTC TCCCACTGGC TATGATTCAC GGAGATCTCC GAGGAGACGT 
CTTCTACCTC CTACCCCACC ATCCCATCGG AGGTCCTCCT TCAACTTTGA GTGTCTGCGC 
AGGCAAAGCA GCCAAGACGA TGTCCTTCCA TCGCCTGCCC TGCCTCACCG CGCTGCCCTG 
CCCCTGCACC TGATGCAGCA GCAGATCATG GCCGTTGCAG GCCTAGATTC CAGTAAAGCC 
CAGAAGTACT CACCGAGCCA CTCTACCCGG TCGTGGGCCA CCCCTCCAGC AACCCCTCCA 
TACCGAGACT GGAGCCCATG CTACACCCCT TTGATCCAGG TGGACCGCTC AGAATCTATG 
GACCAGGTCA ATGGCAGCCT GCCATCCCTA CACCGAAGCT CCTGGTACAC AGATGAACCC 
GACATCTCCT ATAGGACTTT CACGCCAGCT AGTCTGACTG TCCCCAGCAG CTTCCGGAAC 
AAGAACAGCG ACAAGCAGAG GAGTGCAGAC AGCCTGGTGG AGGCAGTCCT GATATCCGAA 
GGCTTGGGAC GCTACGCGAG GGACCCCAAA TTTGTCTCAG CGACAAAACA TGAGATTGCC 
GATGCCTGTG ACCTAACCAT AGACGAGATG GAAAGTGCTG CCAGCACCCT GCTCAATGGC 
AGTGTGTGTC CTCGAGCCAA TGGGGACATG GGCCCCATCT CCCACCGCCA GGACTATGAG 
CTCCAGGACT TTGGTCCTGG CTACAGTGAC GAGGAGCCAG ACCCTGGGCG AGAGGAAGAG 
GACCTGGCAG ATGAGATGAT TTGCATTACC ACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171299.1
CDS	561..7139
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315810.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315810.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAACTTCTCA GCAGGGGGGA AGGCATCCGG ACACTGCTGT GGACCTTCAT CAAGTCCTTT 
CAGGCGCTGC CGTATGTTGC CCTCCTCATT GCCATGCTCT TCTTCATCTA CGCTGTCATA 
GGCATGCAGA TGTTTGGGAA AGTTGCCATG AGAGATAACA ACCAGATCAA TAGGAACAAC 
AACTTCCAGA CCTTTCCTCA GGCAGTGCTC CTACTCTTCA GGTGTGCAAC AGGGGAAGCC 
TGGCAGGAGA TCATGCTTGC CTGCCTCCCA GGAAAGCTGT GCGACCCAGA CTCCGATTAC 
AACCCAGGAG AGGAGTACAC TTGTGGGAGC AACTTTGCCA TTGTCTACTT CATCAGCTTT 
TACATGCTCT GTGCATTCCT GATCATCAAC CTCTTTGTGG CTGTCATCAT GGACAATTTT 
GACTATCTGA CTCGGGACTG GTCTATTCTG GGGCCTCACC ACTTGGACGA ATTCAAAAGA 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AACACCTTGA TGTGGTCACT 
CTGCTCCGGC GGATCCAGCC TCCCCTGGGG TTTGGAAAAT TATGCCCACA CCGAGTAGCA 
TGTAAGAGAT TGGTTGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTCAAT 
GCAACCCTGT TTGCTTTGGT CCGGACAGCT CTCAAGATCA AGACTGAAGG GAACCTGGAG 
CAAGCTAATG AAGAACTCCG AGCTGTGATC AAGAAAATCT GGAAGAAGAC AAGCATGAAG 
CTGCTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA GCAAGGACTG 
GTGGGGAAGT ACCCTGCGAA GAACACCACG ATTGCCCTAC AGGCGGGATT AAGGACCCTG 
CATGACATTG GGCCAGAAAT CCGACGGGCT ATATCCTGTG ACTTGCAAGA TGACGAGCCA 
GAAGACTCCA AACCAGAAGA AGAAGATGTA TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC 
CATGTCAATC ATGTTAATAG TGATAGGAGA GATTCCCTTC AGCAGACCAA TACCACCCAC 
CGTCCCCTGC ATGTCCAAAG GCCTTCAATG CCACCTGCAA GTGATACTGA GAAACCGCTG 
TTTCCTCCAG CAGGAAATTC GGGGTGTCAT AACCATCATA ACCATAATTC CATAGGGAAG 
CAAGCTCCCA CCTCAACAAA TGCCAATCTC AATAATGCCA ATATGTCCAA AGCTGCCCAC 
GGAAAGCCGC CCAGCATTGG GAACCTTGAG CATGTGTCTG AAAATGGGCA TTACTCCTGC 
AAGCATGATC GAGAGCTTCA AAGAAGGTCC AGTATTAAAA GAACCCGCTA TTACGAGACT 
TACATTAGGT CTGAGTCAGG AGATGAGCAA TTTCCAACCA TTTGCCGTGA AGATCCTGAG 
ATACACGGCT ACTTCCGAGA CCCCCGCTGC TTGGGGGAAC AGGAGTATTT CAGTAGCGAG 
GAGTGCTGTG AGGACGACAG CTCTCCCACC TGGAGCAGGC AAAACTACAA CTACTACAAC 
AGGTACCCAG GCAGCTCCAT GGACTTTGAG AGGCCCCGAG GCTACCACCA CCCCCAAGGC 
TTCTTAGAAG ATGATGACTC TCCCACTGGC TATGATTCAC GGAGATCTCC GAGGAGACGT 
CTTCTACCTC CTACCCCACC ATCCCATCGG AGGTCCTCCT TCAACTTTGA GTGTCTGCGC 
AGGCAAAGCA GCCAAGACGA TGTCCTTCCA TCGCCTGCCC TGCCTCACCG CGCTGCCCTG 
CCCCTGCACC TGATGCAGCA GCAGATCATG GCCGTTGCAG GCCTAGATTC CAGTAAAGCC 
CAGAAGTACT CACCGAGCCA CTCTACCCGG TCGTGGGCCA CCCCTCCAGC AACCCCTCCA 
TACCGAGACT GGAGCCCATG CTACACCCCT TTGATCCAGG TGGACCGCTC AGAATCTATG 
GACCAGGTCA ATGGCAGCCT GCCATCCCTA CACCGAAGCT CCTGGTACAC AGATGAACCC 
GACATCTCCT ATAGGACTTT CACGCCAGCT AGTCTGACTG TCCCCAGCAG CTTCCGGAAC 
AAGAACAGCG ACAAGCAGAG GAGTGCAGAC AGCCTGGTGG AGGCAGTCCT GATATCCGAA 
GGCTTGGGAC GCTACGCGAG GGACCCCAAA TTTGTCTCAG CGACAAAACA TGAGATTGCC 
GATGCCTGTG ACCTAACCAT AGACGAGATG GAAAGTGCTG CCAGCACCCT GCTCAATGGC 
AGTGTGTGTC CTCGAGCCAA TGGGGACATG GGCCCCATCT CCCACCGCCA GGACTATGAG 
CTCCAGGACT TTGGTCCTGG CTACAGTGAC GAGGAGCCAG ACCCTGGGCG AGAGGAAGAG 
GACCTGGCAG ATGAGATGAT TTGCATTACC ACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315810.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171299.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X4, mRNA.

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CloneID	OMu78640
Clone ID Related Accession (Same CDS sequence)	XM_017315810.1 , XM_017315810.2
Accession Version	XM_017315810.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315810.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAACTTCTCA GCAGGGGGGA AGGCATCCGG ACACTGCTGT GGACCTTCAT CAAGTCCTTT 
CAGGCGCTGC CGTATGTTGC CCTCCTCATT GCCATGCTCT TCTTCATCTA CGCTGTCATA 
GGCATGCAGA TGTTTGGGAA AGTTGCCATG AGAGATAACA ACCAGATCAA TAGGAACAAC 
AACTTCCAGA CCTTTCCTCA GGCAGTGCTC CTACTCTTCA GGTGTGCAAC AGGGGAAGCC 
TGGCAGGAGA TCATGCTTGC CTGCCTCCCA GGAAAGCTGT GCGACCCAGA CTCCGATTAC 
AACCCAGGAG AGGAGTACAC TTGTGGGAGC AACTTTGCCA TTGTCTACTT CATCAGCTTT 
TACATGCTCT GTGCATTCCT GATCATCAAC CTCTTTGTGG CTGTCATCAT GGACAATTTT 
GACTATCTGA CTCGGGACTG GTCTATTCTG GGGCCTCACC ACTTGGACGA ATTCAAAAGA 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AACACCTTGA TGTGGTCACT 
CTGCTCCGGC GGATCCAGCC TCCCCTGGGG TTTGGAAAAT TATGCCCACA CCGAGTAGCA 
TGTAAGAGAT TGGTTGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTCAAT 
GCAACCCTGT TTGCTTTGGT CCGGACAGCT CTCAAGATCA AGACTGAAGG GAACCTGGAG 
CAAGCTAATG AAGAACTCCG AGCTGTGATC AAGAAAATCT GGAAGAAGAC AAGCATGAAG 
CTGCTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA GCAAGGACTG 
GTGGGGAAGT ACCCTGCGAA GAACACCACG ATTGCCCTAC AGGCGGGATT AAGGACCCTG 
CATGACATTG GGCCAGAAAT CCGACGGGCT ATATCCTGTG ACTTGCAAGA TGACGAGCCA 
GAAGACTCCA AACCAGAAGA AGAAGATGTA TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC 
CATGTCAATC ATGTTAATAG TGATAGGAGA GATTCCCTTC AGCAGACCAA TACCACCCAC 
CGTCCCCTGC ATGTCCAAAG GCCTTCAATG CCACCTGCAA GTGATACTGA GAAACCGCTG 
TTTCCTCCAG CAGGAAATTC GGGGTGTCAT AACCATCATA ACCATAATTC CATAGGGAAG 
CAAGCTCCCA CCTCAACAAA TGCCAATCTC AATAATGCCA ATATGTCCAA AGCTGCCCAC 
GGAAAGCCGC CCAGCATTGG GAACCTTGAG CATGTGTCTG AAAATGGGCA TTACTCCTGC 
AAGCATGATC GAGAGCTTCA AAGAAGGTCC AGTATTAAAA GAACCCGCTA TTACGAGACT 
TACATTAGGT CTGAGTCAGG AGATGAGCAA TTTCCAACCA TTTGCCGTGA AGATCCTGAG 
ATACACGGCT ACTTCCGAGA CCCCCGCTGC TTGGGGGAAC AGGAGTATTT CAGTAGCGAG 
GAGTGCTGTG AGGACGACAG CTCTCCCACC TGGAGCAGGC AAAACTACAA CTACTACAAC 
AGGTACCCAG GCAGCTCCAT GGACTTTGAG AGGCCCCGAG GCTACCACCA CCCCCAAGGC 
TTCTTAGAAG ATGATGACTC TCCCACTGGC TATGATTCAC GGAGATCTCC GAGGAGACGT 
CTTCTACCTC CTACCCCACC ATCCCATCGG AGGTCCTCCT TCAACTTTGA GTGTCTGCGC 
AGGCAAAGCA GCCAAGACGA TGTCCTTCCA TCGCCTGCCC TGCCTCACCG CGCTGCCCTG 
CCCCTGCACC TGATGCAGCA GCAGATCATG GCCGTTGCAG GCCTAGATTC CAGTAAAGCC 
CAGAAGTACT CACCGAGCCA CTCTACCCGG TCGTGGGCCA CCCCTCCAGC AACCCCTCCA 
TACCGAGACT GGAGCCCATG CTACACCCCT TTGATCCAGG TGGACCGCTC AGAATCTATG 
GACCAGGTCA ATGGCAGCCT GCCATCCCTA CACCGAAGCT CCTGGTACAC AGATGAACCC 
GACATCTCCT ATAGGACTTT CACGCCAGCT AGTCTGACTG TCCCCAGCAG CTTCCGGAAC 
AAGAACAGCG ACAAGCAGAG GAGTGCAGAC AGCCTGGTGG AGGCAGTCCT GATATCCGAA 
GGCTTGGGAC GCTACGCGAG GGACCCCAAA TTTGTCTCAG CGACAAAACA TGAGATTGCC 
GATGCCTGTG ACCTAACCAT AGACGAGATG GAAAGTGCTG CCAGCACCCT GCTCAATGGC 
AGTGTGTGTC CTCGAGCCAA TGGGGACATG GGCCCCATCT CCCACCGCCA GGACTATGAG 
CTCCAGGACT TTGGTCCTGG CTACAGTGAC GAGGAGCCAG ACCCTGGGCG AGAGGAAGAG 
GACCTGGCAG ATGAGATGAT TTGCATTACC ACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171299.1
CDS	494..7072
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315810.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315810.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAACTTCTCA GCAGGGGGGA AGGCATCCGG ACACTGCTGT GGACCTTCAT CAAGTCCTTT 
CAGGCGCTGC CGTATGTTGC CCTCCTCATT GCCATGCTCT TCTTCATCTA CGCTGTCATA 
GGCATGCAGA TGTTTGGGAA AGTTGCCATG AGAGATAACA ACCAGATCAA TAGGAACAAC 
AACTTCCAGA CCTTTCCTCA GGCAGTGCTC CTACTCTTCA GGTGTGCAAC AGGGGAAGCC 
TGGCAGGAGA TCATGCTTGC CTGCCTCCCA GGAAAGCTGT GCGACCCAGA CTCCGATTAC 
AACCCAGGAG AGGAGTACAC TTGTGGGAGC AACTTTGCCA TTGTCTACTT CATCAGCTTT 
TACATGCTCT GTGCATTCCT GATCATCAAC CTCTTTGTGG CTGTCATCAT GGACAATTTT 
GACTATCTGA CTCGGGACTG GTCTATTCTG GGGCCTCACC ACTTGGACGA ATTCAAAAGA 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AACACCTTGA TGTGGTCACT 
CTGCTCCGGC GGATCCAGCC TCCCCTGGGG TTTGGAAAAT TATGCCCACA CCGAGTAGCA 
TGTAAGAGAT TGGTTGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTCAAT 
GCAACCCTGT TTGCTTTGGT CCGGACAGCT CTCAAGATCA AGACTGAAGG GAACCTGGAG 
CAAGCTAATG AAGAACTCCG AGCTGTGATC AAGAAAATCT GGAAGAAGAC AAGCATGAAG 
CTGCTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA GCAAGGACTG 
GTGGGGAAGT ACCCTGCGAA GAACACCACG ATTGCCCTAC AGGCGGGATT AAGGACCCTG 
CATGACATTG GGCCAGAAAT CCGACGGGCT ATATCCTGTG ACTTGCAAGA TGACGAGCCA 
GAAGACTCCA AACCAGAAGA AGAAGATGTA TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC 
CATGTCAATC ATGTTAATAG TGATAGGAGA GATTCCCTTC AGCAGACCAA TACCACCCAC 
CGTCCCCTGC ATGTCCAAAG GCCTTCAATG CCACCTGCAA GTGATACTGA GAAACCGCTG 
TTTCCTCCAG CAGGAAATTC GGGGTGTCAT AACCATCATA ACCATAATTC CATAGGGAAG 
CAAGCTCCCA CCTCAACAAA TGCCAATCTC AATAATGCCA ATATGTCCAA AGCTGCCCAC 
GGAAAGCCGC CCAGCATTGG GAACCTTGAG CATGTGTCTG AAAATGGGCA TTACTCCTGC 
AAGCATGATC GAGAGCTTCA AAGAAGGTCC AGTATTAAAA GAACCCGCTA TTACGAGACT 
TACATTAGGT CTGAGTCAGG AGATGAGCAA TTTCCAACCA TTTGCCGTGA AGATCCTGAG 
ATACACGGCT ACTTCCGAGA CCCCCGCTGC TTGGGGGAAC AGGAGTATTT CAGTAGCGAG 
GAGTGCTGTG AGGACGACAG CTCTCCCACC TGGAGCAGGC AAAACTACAA CTACTACAAC 
AGGTACCCAG GCAGCTCCAT GGACTTTGAG AGGCCCCGAG GCTACCACCA CCCCCAAGGC 
TTCTTAGAAG ATGATGACTC TCCCACTGGC TATGATTCAC GGAGATCTCC GAGGAGACGT 
CTTCTACCTC CTACCCCACC ATCCCATCGG AGGTCCTCCT TCAACTTTGA GTGTCTGCGC 
AGGCAAAGCA GCCAAGACGA TGTCCTTCCA TCGCCTGCCC TGCCTCACCG CGCTGCCCTG 
CCCCTGCACC TGATGCAGCA GCAGATCATG GCCGTTGCAG GCCTAGATTC CAGTAAAGCC 
CAGAAGTACT CACCGAGCCA CTCTACCCGG TCGTGGGCCA CCCCTCCAGC AACCCCTCCA 
TACCGAGACT GGAGCCCATG CTACACCCCT TTGATCCAGG TGGACCGCTC AGAATCTATG 
GACCAGGTCA ATGGCAGCCT GCCATCCCTA CACCGAAGCT CCTGGTACAC AGATGAACCC 
GACATCTCCT ATAGGACTTT CACGCCAGCT AGTCTGACTG TCCCCAGCAG CTTCCGGAAC 
AAGAACAGCG ACAAGCAGAG GAGTGCAGAC AGCCTGGTGG AGGCAGTCCT GATATCCGAA 
GGCTTGGGAC GCTACGCGAG GGACCCCAAA TTTGTCTCAG CGACAAAACA TGAGATTGCC 
GATGCCTGTG ACCTAACCAT AGACGAGATG GAAAGTGCTG CCAGCACCCT GCTCAATGGC 
AGTGTGTGTC CTCGAGCCAA TGGGGACATG GGCCCCATCT CCCACCGCCA GGACTATGAG 
CTCCAGGACT TTGGTCCTGG CTACAGTGAC GAGGAGCCAG ACCCTGGGCG AGAGGAAGAG 
GACCTGGCAG ATGAGATGAT TTGCATTACC ACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315811.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171300.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X5, mRNA.

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CloneID	OMu78641
Clone ID Related Accession (Same CDS sequence)	XM_017315811.1 , XM_017315811.2
Accession Version	XM_017315811.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171300.1
CDS	561..7124
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315811.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315811.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315811.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171300.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X5, mRNA.

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CloneID	OMu78641
Clone ID Related Accession (Same CDS sequence)	XM_017315811.1 , XM_017315811.2
Accession Version	XM_017315811.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315811.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171300.1
CDS	494..7057
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315811.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315811.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315812.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171301.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X6, mRNA.

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CloneID	OMu78642
Clone ID Related Accession (Same CDS sequence)	XM_017315812.1 , XM_017315812.2
Accession Version	XM_017315812.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171301.1
CDS	561..7124
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315812.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315812.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315812.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171301.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X6, mRNA.

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CloneID	OMu78642
Clone ID Related Accession (Same CDS sequence)	XM_017315812.1 , XM_017315812.2
Accession Version	XM_017315812.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315812.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171301.1
CDS	494..7057
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315812.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315812.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315813.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171302.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X7, mRNA.

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CloneID	OMu78643
Clone ID Related Accession (Same CDS sequence)	XM_017315813.2 , XM_017315813.1
Accession Version	XM_017315813.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315813.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171302.1
CDS	494..7033
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315813.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315813.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315813.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171302.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X7, mRNA.

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CloneID	OMu78643
Clone ID Related Accession (Same CDS sequence)	XM_017315813.2 , XM_017315813.1
Accession Version	XM_017315813.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171302.1
CDS	561..7100
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315813.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315813.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315814.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171303.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X8, mRNA.

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CloneID	OMu78644
Clone ID Related Accession (Same CDS sequence)	XM_017315814.2 , XM_017315814.1
Accession Version	XM_017315814.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315814.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171303.1
CDS	494..7033
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315814.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315814.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315814.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171303.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X8, mRNA.

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CloneID	OMu78644
Clone ID Related Accession (Same CDS sequence)	XM_017315814.2 , XM_017315814.1
Accession Version	XM_017315814.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171303.1
CDS	561..7100
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315814.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315814.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315815.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171304.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X9, mRNA.

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CloneID	OMu78645
Clone ID Related Accession (Same CDS sequence)	XM_017315815.2 , XM_017315815.1
Accession Version	XM_017315815.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315815.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171304.1
CDS	494..6988
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315815.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315815.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315815.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171304.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X9, mRNA.

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CloneID	OMu78645
Clone ID Related Accession (Same CDS sequence)	XM_017315815.2 , XM_017315815.1
Accession Version	XM_017315815.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171304.1
CDS	561..7055
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315815.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315815.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315816.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171305.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X10, mRNA.

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CloneID	OMu78646
Clone ID Related Accession (Same CDS sequence)	XM_017315816.1 , XM_017315816.2
Accession Version	XM_017315816.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171305.1
CDS	561..7055
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315816.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315816.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315816.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171305.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X10, mRNA.

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CloneID	OMu78646
Clone ID Related Accession (Same CDS sequence)	XM_017315816.1 , XM_017315816.2
Accession Version	XM_017315816.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315816.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171305.1
CDS	494..6988
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315816.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315816.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315817.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171306.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X12, mRNA.

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CloneID	OMu78647
Clone ID Related Accession (Same CDS sequence)	XM_017315817.2 , XM_017315817.1
Accession Version	XM_017315817.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCCAT CTGAAAGTGA GACTATCCCT CTCCCAACTG CCACACCTGG GAACTCTGAA 
GAGAGCAATA GAATCTCCAT CACCTTTTTC CGTCTTTTCC GAGTGATGCG GTTGGTGAAA 
CTTCTCAGCA GGGGGGAAGG CATCCGGACA CTGCTGTGGA CCTTCATCAA GTCCTTTCAG 
GCGCTGCCGT ATGTTGCCCT CCTCATTGCC ATGCTCTTCT TCATCTACGC TGTCATAGGC 
ATGCAGATGT TTGGGAAAGT TGCCATGAGA GATAACAACC AGATCAATAG GAACAACAAC 
TTCCAGACCT TTCCTCAGGC AGTGCTCCTA CTCTTCAGGT GTGCAACAGG GGAAGCCTGG 
CAGGAGATCA TGCTTGCCTG CCTCCCAGGA AAGCTGTGCG ACCCAGACTC CGATTACAAC 
CCAGGAGAGG AGTACACTTG TGGGAGCAAC TTTGCCATTG TCTACTTCAT CAGCTTTTAC 
ATGCTCTGTG CATTCCTGAT CATCAACCTC TTTGTGGCTG TCATCATGGA CAATTTTGAC 
TATCTGACTC GGGACTGGTC TATTCTGGGG CCTCACCACT TGGACGAATT CAAAAGAATA 
TGGTCAGAAT ATGACCCTGA AGCAAAGGGA AGGATAAAAC ACCTTGATGT GGTCACTCTG 
CTCCGGCGGA TCCAGCCTCC CCTGGGGTTT GGAAAATTAT GCCCACACCG AGTAGCATGT 
AAGAGATTGG TTGCCATGAA CATGCCTCTC AACAGTGATG GGACAGTCAT GTTCAATGCA 
ACCCTGTTTG CTTTGGTCCG GACAGCTCTC AAGATCAAGA CTGAAGGGAA CCTGGAGCAA 
GCTAATGAAG AACTCCGAGC TGTGATCAAG AAAATCTGGA AGAAGACAAG CATGAAGCTG 
CTTGACCAAG TTGTCCCTCC AGCTGGTGAT GATGAGGTAA CCGTGGGGAA GTTCTATGCC 
ACTTTCCTGA TACAGGACTA CTTTAGGAAA TTCAAGAAAC GGAAAGAGCA AGGACTGGTG 
GGGAAGTACC CTGCGAAGAA CACCACGATT GCCCTACAGG CGGGATTAAG GACCCTGCAT 
GACATTGGGC CAGAAATCCG ACGGGCTATA TCCTGTGACT TGCAAGATGA CGAGCCAGAA 
GACTCCAAAC CAGAAGAAGA AGATGTATTC AAAAGAAATG GTGCCCTGCT TGGAAACCAT 
GTCAATCATG TTAATAGTGA TAGGAGAGAT TCCCTTCAGC AGACCAATAC CACCCACCGT 
CCCCTGCATG TCCAAAGGCC TTCAATGCCA CCTGCAAGTG ATACTGAGAA ACCGCTGTTT 
CCTCCAGCAG GAAATTCGGG GTGTCATAAC CATCATAACC ATAATTCCAT AGGGAAGCAA 
GCTCCCACCT CAACAAATGC CAATCTCAAT AATGCCAATA TGTCCAAAGC TGCCCACGGA 
AAGCCGCCCA GCATTGGGAA CCTTGAGCAT GTGTCTGAAA ATGGGCATTA CTCCTGCAAG 
CATGATCGAG AGCTTCAAAG AAGGTCCAGT ATTAAAAGAA CCCGCTATTA CGAGACTTAC 
ATTAGGTCTG AGTCAGGAGA TGAGCAATTT CCAACCATTT GCCGTGAAGA TCCTGAGATA 
CACGGCTACT TCCGAGACCC CCGCTGCTTG GGGGAACAGG AGTATTTCAG TAGCGAGGAG 
TGCTGTGAGG ACGACAGCTC TCCCACCTGG AGCAGGCAAA ACTACAACTA CTACAACAGG 
TACCCAGGCA GCTCCATGGA CTTTGAGAGG CCCCGAGGCT ACCACCACCC CCAAGGCTTC 
TTAGAAGATG ATGACTCTCC CACTGGCTAT GATTCACGGA GATCTCCGAG GAGACGTCTT 
CTACCTCCTA CCCCACCATC CCATCGGAGG TCCTCCTTCA ACTTTGAGTG TCTGCGCAGG 
CAAAGCAGCC AAGACGATGT CCTTCCATCG CCTGCCCTGC CTCACCGCGC TGCCCTGCCC 
CTGCACCTGA TGCAGCAGCA GATCATGGCC GTTGCAGGCC TAGATTCCAG TAAAGCCCAG 
AAGTACTCAC CGAGCCACTC TACCCGGTCG TGGGCCACCC CTCCAGCAAC CCCTCCATAC 
CGAGACTGGA GCCCATGCTA CACCCCTTTG ATCCAGGTGG ACCGCTCAGA ATCTATGGAC 
CAGGTCAATG GCAGCCTGCC ATCCCTACAC CGAAGCTCCT GGTACACAGA TGAACCCGAC 
ATCTCCTATA GGACTTTCAC GCCAGCTAGT CTGACTGTCC CCAGCAGCTT CCGGAACAAG 
AACAGCGACA AGCAGAGGAG TGCAGACAGC CTGGTGGAGG CAGTCCTGAT ATCCGAAGGC 
TTGGGACGCT ACGCGAGGGA CCCCAAATTT GTCTCAGCGA CAAAACATGA GATTGCCGAT 
GCCTGTGACC TAACCATAGA CGAGATGGAA AGTGCTGCCA GCACCCTGCT CAATGGCAGT 
GTGTGTCCTC GAGCCAATGG GGACATGGGC CCCATCTCCC ACCGCCAGGA CTATGAGCTC 
CAGGACTTTG GTCCTGGCTA CAGTGACGAG GAGCCAGACC CTGGGCGAGA GGAAGAGGAC 
CTGGCAGATG AGATGATTTG CATTACCACC TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171306.1
CDS	494..6949
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315817.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315817.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCCAT CTGAAAGTGA GACTATCCCT CTCCCAACTG CCACACCTGG GAACTCTGAA 
GAGAGCAATA GAATCTCCAT CACCTTTTTC CGTCTTTTCC GAGTGATGCG GTTGGTGAAA 
CTTCTCAGCA GGGGGGAAGG CATCCGGACA CTGCTGTGGA CCTTCATCAA GTCCTTTCAG 
GCGCTGCCGT ATGTTGCCCT CCTCATTGCC ATGCTCTTCT TCATCTACGC TGTCATAGGC 
ATGCAGATGT TTGGGAAAGT TGCCATGAGA GATAACAACC AGATCAATAG GAACAACAAC 
TTCCAGACCT TTCCTCAGGC AGTGCTCCTA CTCTTCAGGT GTGCAACAGG GGAAGCCTGG 
CAGGAGATCA TGCTTGCCTG CCTCCCAGGA AAGCTGTGCG ACCCAGACTC CGATTACAAC 
CCAGGAGAGG AGTACACTTG TGGGAGCAAC TTTGCCATTG TCTACTTCAT CAGCTTTTAC 
ATGCTCTGTG CATTCCTGAT CATCAACCTC TTTGTGGCTG TCATCATGGA CAATTTTGAC 
TATCTGACTC GGGACTGGTC TATTCTGGGG CCTCACCACT TGGACGAATT CAAAAGAATA 
TGGTCAGAAT ATGACCCTGA AGCAAAGGGA AGGATAAAAC ACCTTGATGT GGTCACTCTG 
CTCCGGCGGA TCCAGCCTCC CCTGGGGTTT GGAAAATTAT GCCCACACCG AGTAGCATGT 
AAGAGATTGG TTGCCATGAA CATGCCTCTC AACAGTGATG GGACAGTCAT GTTCAATGCA 
ACCCTGTTTG CTTTGGTCCG GACAGCTCTC AAGATCAAGA CTGAAGGGAA CCTGGAGCAA 
GCTAATGAAG AACTCCGAGC TGTGATCAAG AAAATCTGGA AGAAGACAAG CATGAAGCTG 
CTTGACCAAG TTGTCCCTCC AGCTGGTGAT GATGAGGTAA CCGTGGGGAA GTTCTATGCC 
ACTTTCCTGA TACAGGACTA CTTTAGGAAA TTCAAGAAAC GGAAAGAGCA AGGACTGGTG 
GGGAAGTACC CTGCGAAGAA CACCACGATT GCCCTACAGG CGGGATTAAG GACCCTGCAT 
GACATTGGGC CAGAAATCCG ACGGGCTATA TCCTGTGACT TGCAAGATGA CGAGCCAGAA 
GACTCCAAAC CAGAAGAAGA AGATGTATTC AAAAGAAATG GTGCCCTGCT TGGAAACCAT 
GTCAATCATG TTAATAGTGA TAGGAGAGAT TCCCTTCAGC AGACCAATAC CACCCACCGT 
CCCCTGCATG TCCAAAGGCC TTCAATGCCA CCTGCAAGTG ATACTGAGAA ACCGCTGTTT 
CCTCCAGCAG GAAATTCGGG GTGTCATAAC CATCATAACC ATAATTCCAT AGGGAAGCAA 
GCTCCCACCT CAACAAATGC CAATCTCAAT AATGCCAATA TGTCCAAAGC TGCCCACGGA 
AAGCCGCCCA GCATTGGGAA CCTTGAGCAT GTGTCTGAAA ATGGGCATTA CTCCTGCAAG 
CATGATCGAG AGCTTCAAAG AAGGTCCAGT ATTAAAAGAA CCCGCTATTA CGAGACTTAC 
ATTAGGTCTG AGTCAGGAGA TGAGCAATTT CCAACCATTT GCCGTGAAGA TCCTGAGATA 
CACGGCTACT TCCGAGACCC CCGCTGCTTG GGGGAACAGG AGTATTTCAG TAGCGAGGAG 
TGCTGTGAGG ACGACAGCTC TCCCACCTGG AGCAGGCAAA ACTACAACTA CTACAACAGG 
TACCCAGGCA GCTCCATGGA CTTTGAGAGG CCCCGAGGCT ACCACCACCC CCAAGGCTTC 
TTAGAAGATG ATGACTCTCC CACTGGCTAT GATTCACGGA GATCTCCGAG GAGACGTCTT 
CTACCTCCTA CCCCACCATC CCATCGGAGG TCCTCCTTCA ACTTTGAGTG TCTGCGCAGG 
CAAAGCAGCC AAGACGATGT CCTTCCATCG CCTGCCCTGC CTCACCGCGC TGCCCTGCCC 
CTGCACCTGA TGCAGCAGCA GATCATGGCC GTTGCAGGCC TAGATTCCAG TAAAGCCCAG 
AAGTACTCAC CGAGCCACTC TACCCGGTCG TGGGCCACCC CTCCAGCAAC CCCTCCATAC 
CGAGACTGGA GCCCATGCTA CACCCCTTTG ATCCAGGTGG ACCGCTCAGA ATCTATGGAC 
CAGGTCAATG GCAGCCTGCC ATCCCTACAC CGAAGCTCCT GGTACACAGA TGAACCCGAC 
ATCTCCTATA GGACTTTCAC GCCAGCTAGT CTGACTGTCC CCAGCAGCTT CCGGAACAAG 
AACAGCGACA AGCAGAGGAG TGCAGACAGC CTGGTGGAGG CAGTCCTGAT ATCCGAAGGC 
TTGGGACGCT ACGCGAGGGA CCCCAAATTT GTCTCAGCGA CAAAACATGA GATTGCCGAT 
GCCTGTGACC TAACCATAGA CGAGATGGAA AGTGCTGCCA GCACCCTGCT CAATGGCAGT 
GTGTGTCCTC GAGCCAATGG GGACATGGGC CCCATCTCCC ACCGCCAGGA CTATGAGCTC 
CAGGACTTTG GTCCTGGCTA CAGTGACGAG GAGCCAGACC CTGGGCGAGA GGAAGAGGAC 
CTGGCAGATG AGATGATTTG CATTACCACC TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315817.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171306.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X12, mRNA.

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CloneID	OMu78647
Clone ID Related Accession (Same CDS sequence)	XM_017315817.2 , XM_017315817.1
Accession Version	XM_017315817.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCCAT CTGAAAGTGA GACTATCCCT CTCCCAACTG CCACACCTGG GAACTCTGAA 
GAGAGCAATA GAATCTCCAT CACCTTTTTC CGTCTTTTCC GAGTGATGCG GTTGGTGAAA 
CTTCTCAGCA GGGGGGAAGG CATCCGGACA CTGCTGTGGA CCTTCATCAA GTCCTTTCAG 
GCGCTGCCGT ATGTTGCCCT CCTCATTGCC ATGCTCTTCT TCATCTACGC TGTCATAGGC 
ATGCAGATGT TTGGGAAAGT TGCCATGAGA GATAACAACC AGATCAATAG GAACAACAAC 
TTCCAGACCT TTCCTCAGGC AGTGCTCCTA CTCTTCAGGT GTGCAACAGG GGAAGCCTGG 
CAGGAGATCA TGCTTGCCTG CCTCCCAGGA AAGCTGTGCG ACCCAGACTC CGATTACAAC 
CCAGGAGAGG AGTACACTTG TGGGAGCAAC TTTGCCATTG TCTACTTCAT CAGCTTTTAC 
ATGCTCTGTG CATTCCTGAT CATCAACCTC TTTGTGGCTG TCATCATGGA CAATTTTGAC 
TATCTGACTC GGGACTGGTC TATTCTGGGG CCTCACCACT TGGACGAATT CAAAAGAATA 
TGGTCAGAAT ATGACCCTGA AGCAAAGGGA AGGATAAAAC ACCTTGATGT GGTCACTCTG 
CTCCGGCGGA TCCAGCCTCC CCTGGGGTTT GGAAAATTAT GCCCACACCG AGTAGCATGT 
AAGAGATTGG TTGCCATGAA CATGCCTCTC AACAGTGATG GGACAGTCAT GTTCAATGCA 
ACCCTGTTTG CTTTGGTCCG GACAGCTCTC AAGATCAAGA CTGAAGGGAA CCTGGAGCAA 
GCTAATGAAG AACTCCGAGC TGTGATCAAG AAAATCTGGA AGAAGACAAG CATGAAGCTG 
CTTGACCAAG TTGTCCCTCC AGCTGGTGAT GATGAGGTAA CCGTGGGGAA GTTCTATGCC 
ACTTTCCTGA TACAGGACTA CTTTAGGAAA TTCAAGAAAC GGAAAGAGCA AGGACTGGTG 
GGGAAGTACC CTGCGAAGAA CACCACGATT GCCCTACAGG CGGGATTAAG GACCCTGCAT 
GACATTGGGC CAGAAATCCG ACGGGCTATA TCCTGTGACT TGCAAGATGA CGAGCCAGAA 
GACTCCAAAC CAGAAGAAGA AGATGTATTC AAAAGAAATG GTGCCCTGCT TGGAAACCAT 
GTCAATCATG TTAATAGTGA TAGGAGAGAT TCCCTTCAGC AGACCAATAC CACCCACCGT 
CCCCTGCATG TCCAAAGGCC TTCAATGCCA CCTGCAAGTG ATACTGAGAA ACCGCTGTTT 
CCTCCAGCAG GAAATTCGGG GTGTCATAAC CATCATAACC ATAATTCCAT AGGGAAGCAA 
GCTCCCACCT CAACAAATGC CAATCTCAAT AATGCCAATA TGTCCAAAGC TGCCCACGGA 
AAGCCGCCCA GCATTGGGAA CCTTGAGCAT GTGTCTGAAA ATGGGCATTA CTCCTGCAAG 
CATGATCGAG AGCTTCAAAG AAGGTCCAGT ATTAAAAGAA CCCGCTATTA CGAGACTTAC 
ATTAGGTCTG AGTCAGGAGA TGAGCAATTT CCAACCATTT GCCGTGAAGA TCCTGAGATA 
CACGGCTACT TCCGAGACCC CCGCTGCTTG GGGGAACAGG AGTATTTCAG TAGCGAGGAG 
TGCTGTGAGG ACGACAGCTC TCCCACCTGG AGCAGGCAAA ACTACAACTA CTACAACAGG 
TACCCAGGCA GCTCCATGGA CTTTGAGAGG CCCCGAGGCT ACCACCACCC CCAAGGCTTC 
TTAGAAGATG ATGACTCTCC CACTGGCTAT GATTCACGGA GATCTCCGAG GAGACGTCTT 
CTACCTCCTA CCCCACCATC CCATCGGAGG TCCTCCTTCA ACTTTGAGTG TCTGCGCAGG 
CAAAGCAGCC AAGACGATGT CCTTCCATCG CCTGCCCTGC CTCACCGCGC TGCCCTGCCC 
CTGCACCTGA TGCAGCAGCA GATCATGGCC GTTGCAGGCC TAGATTCCAG TAAAGCCCAG 
AAGTACTCAC CGAGCCACTC TACCCGGTCG TGGGCCACCC CTCCAGCAAC CCCTCCATAC 
CGAGACTGGA GCCCATGCTA CACCCCTTTG ATCCAGGTGG ACCGCTCAGA ATCTATGGAC 
CAGGTCAATG GCAGCCTGCC ATCCCTACAC CGAAGCTCCT GGTACACAGA TGAACCCGAC 
ATCTCCTATA GGACTTTCAC GCCAGCTAGT CTGACTGTCC CCAGCAGCTT CCGGAACAAG 
AACAGCGACA AGCAGAGGAG TGCAGACAGC CTGGTGGAGG CAGTCCTGAT ATCCGAAGGC 
TTGGGACGCT ACGCGAGGGA CCCCAAATTT GTCTCAGCGA CAAAACATGA GATTGCCGAT 
GCCTGTGACC TAACCATAGA CGAGATGGAA AGTGCTGCCA GCACCCTGCT CAATGGCAGT 
GTGTGTCCTC GAGCCAATGG GGACATGGGC CCCATCTCCC ACCGCCAGGA CTATGAGCTC 
CAGGACTTTG GTCCTGGCTA CAGTGACGAG GAGCCAGACC CTGGGCGAGA GGAAGAGGAC 
CTGGCAGATG AGATGATTTG CATTACCACC TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171306.1
CDS	561..7016
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315817.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315817.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCCAT CTGAAAGTGA GACTATCCCT CTCCCAACTG CCACACCTGG GAACTCTGAA 
GAGAGCAATA GAATCTCCAT CACCTTTTTC CGTCTTTTCC GAGTGATGCG GTTGGTGAAA 
CTTCTCAGCA GGGGGGAAGG CATCCGGACA CTGCTGTGGA CCTTCATCAA GTCCTTTCAG 
GCGCTGCCGT ATGTTGCCCT CCTCATTGCC ATGCTCTTCT TCATCTACGC TGTCATAGGC 
ATGCAGATGT TTGGGAAAGT TGCCATGAGA GATAACAACC AGATCAATAG GAACAACAAC 
TTCCAGACCT TTCCTCAGGC AGTGCTCCTA CTCTTCAGGT GTGCAACAGG GGAAGCCTGG 
CAGGAGATCA TGCTTGCCTG CCTCCCAGGA AAGCTGTGCG ACCCAGACTC CGATTACAAC 
CCAGGAGAGG AGTACACTTG TGGGAGCAAC TTTGCCATTG TCTACTTCAT CAGCTTTTAC 
ATGCTCTGTG CATTCCTGAT CATCAACCTC TTTGTGGCTG TCATCATGGA CAATTTTGAC 
TATCTGACTC GGGACTGGTC TATTCTGGGG CCTCACCACT TGGACGAATT CAAAAGAATA 
TGGTCAGAAT ATGACCCTGA AGCAAAGGGA AGGATAAAAC ACCTTGATGT GGTCACTCTG 
CTCCGGCGGA TCCAGCCTCC CCTGGGGTTT GGAAAATTAT GCCCACACCG AGTAGCATGT 
AAGAGATTGG TTGCCATGAA CATGCCTCTC AACAGTGATG GGACAGTCAT GTTCAATGCA 
ACCCTGTTTG CTTTGGTCCG GACAGCTCTC AAGATCAAGA CTGAAGGGAA CCTGGAGCAA 
GCTAATGAAG AACTCCGAGC TGTGATCAAG AAAATCTGGA AGAAGACAAG CATGAAGCTG 
CTTGACCAAG TTGTCCCTCC AGCTGGTGAT GATGAGGTAA CCGTGGGGAA GTTCTATGCC 
ACTTTCCTGA TACAGGACTA CTTTAGGAAA TTCAAGAAAC GGAAAGAGCA AGGACTGGTG 
GGGAAGTACC CTGCGAAGAA CACCACGATT GCCCTACAGG CGGGATTAAG GACCCTGCAT 
GACATTGGGC CAGAAATCCG ACGGGCTATA TCCTGTGACT TGCAAGATGA CGAGCCAGAA 
GACTCCAAAC CAGAAGAAGA AGATGTATTC AAAAGAAATG GTGCCCTGCT TGGAAACCAT 
GTCAATCATG TTAATAGTGA TAGGAGAGAT TCCCTTCAGC AGACCAATAC CACCCACCGT 
CCCCTGCATG TCCAAAGGCC TTCAATGCCA CCTGCAAGTG ATACTGAGAA ACCGCTGTTT 
CCTCCAGCAG GAAATTCGGG GTGTCATAAC CATCATAACC ATAATTCCAT AGGGAAGCAA 
GCTCCCACCT CAACAAATGC CAATCTCAAT AATGCCAATA TGTCCAAAGC TGCCCACGGA 
AAGCCGCCCA GCATTGGGAA CCTTGAGCAT GTGTCTGAAA ATGGGCATTA CTCCTGCAAG 
CATGATCGAG AGCTTCAAAG AAGGTCCAGT ATTAAAAGAA CCCGCTATTA CGAGACTTAC 
ATTAGGTCTG AGTCAGGAGA TGAGCAATTT CCAACCATTT GCCGTGAAGA TCCTGAGATA 
CACGGCTACT TCCGAGACCC CCGCTGCTTG GGGGAACAGG AGTATTTCAG TAGCGAGGAG 
TGCTGTGAGG ACGACAGCTC TCCCACCTGG AGCAGGCAAA ACTACAACTA CTACAACAGG 
TACCCAGGCA GCTCCATGGA CTTTGAGAGG CCCCGAGGCT ACCACCACCC CCAAGGCTTC 
TTAGAAGATG ATGACTCTCC CACTGGCTAT GATTCACGGA GATCTCCGAG GAGACGTCTT 
CTACCTCCTA CCCCACCATC CCATCGGAGG TCCTCCTTCA ACTTTGAGTG TCTGCGCAGG 
CAAAGCAGCC AAGACGATGT CCTTCCATCG CCTGCCCTGC CTCACCGCGC TGCCCTGCCC 
CTGCACCTGA TGCAGCAGCA GATCATGGCC GTTGCAGGCC TAGATTCCAG TAAAGCCCAG 
AAGTACTCAC CGAGCCACTC TACCCGGTCG TGGGCCACCC CTCCAGCAAC CCCTCCATAC 
CGAGACTGGA GCCCATGCTA CACCCCTTTG ATCCAGGTGG ACCGCTCAGA ATCTATGGAC 
CAGGTCAATG GCAGCCTGCC ATCCCTACAC CGAAGCTCCT GGTACACAGA TGAACCCGAC 
ATCTCCTATA GGACTTTCAC GCCAGCTAGT CTGACTGTCC CCAGCAGCTT CCGGAACAAG 
AACAGCGACA AGCAGAGGAG TGCAGACAGC CTGGTGGAGG CAGTCCTGAT ATCCGAAGGC 
TTGGGACGCT ACGCGAGGGA CCCCAAATTT GTCTCAGCGA CAAAACATGA GATTGCCGAT 
GCCTGTGACC TAACCATAGA CGAGATGGAA AGTGCTGCCA GCACCCTGCT CAATGGCAGT 
GTGTGTCCTC GAGCCAATGG GGACATGGGC CCCATCTCCC ACCGCCAGGA CTATGAGCTC 
CAGGACTTTG GTCCTGGCTA CAGTGACGAG GAGCCAGACC CTGGGCGAGA GGAAGAGGAC 
CTGGCAGATG AGATGATTTG CATTACCACC TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315818.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171307.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X13, mRNA.

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CloneID	OMu78648
Clone ID Related Accession (Same CDS sequence)	XM_017315818.1 , XM_017315818.2
Accession Version	XM_017315818.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCT CTGCACCCCC ACCTGTAGGA TCTCTCTCCC AAAGAAAACG TCAGCAATAC 
GCCAAGAGCA AAAAACAGGG TAACTCGTCT AACAGCCGAC CTGCCCGTGC CCTTTTCTGT 
TTATCCCTCA ATAACCCCAT CCGAAGAGCC TGCATTAGTA TAGTGGAATG GAAACCATTT 
GACATATTTA TATTATTGGC TATTTTTGCC AATTGTGTGG CCTTAGCTAT TTACATCCCA 
TTCCCTGAAG ATGATTCTAA TTCAACAAAT CATAACTTGG AAAAAGTAGA ATATGCCTTC 
CTGATTATTT TTACAGTGGA GACATTTCTG AAGATTATAG CGTATGGACT GTTGCTGCAT 
CCTAATGCTT ACGTTAGGAA TGGATGGAAT TTACTGGATT TTGTTATAGT AATTGTAGGA 
TTGTTTAGTG TAATTCTGGA ACAATTAACC AAAGAAACAG AAGGCGGGAA CCACTCTAGT 
GGCAAATCAG GAGGCTTTGA TGTCAAAGCC CTCCGTGCCT TCCGAGTGTT AAGACCACTT 
CGACTTGTAT CAGGAGTGCC CAGTTTACAA GTTGTTCTGA ACTCCATTAT AAAAGCCATG 
GTCCCCCTCC TTCACATAGC CCTTTTGGTA TTATTTGTAA TTATCATCTA TGCTATTATA 
GGATTGGAAC TTTTTATTGG AAAAATGCAC AAAACATGTT TTTTTGCTGA CTCAGATATA 
GTAGCTGAAG AGGACCCAGC TCCGTGTGCG TTCTCAGGGA ATGGACGCCA GTGCACCGCC 
AATGGCACGG AATGTAGGAG TGGCTGGGTT GGCCCCAATG GAGGCATCAC TAACTTTGAT 
AACTTTGCCT TTGCCATGCT CACTGTGTTC CAGTGCATTA CCATGGAGGG CTGGACAGAT 
GTGCTCTACT GGGTTAATGA TGCGATAGGA TGGGAATGGC CATGGGTGTA TTTTGTTAGT 
CTGATCATCC TTGGCTCATT TTTCGTCCTT AACCTGGTTC TTGGTGTCCT TAGTGGAGAA 
TTCTCCAAGG AAAGAGAGAA GGCTAAAGCT CGTGGAGATT TCCAGAAGCT TCGGGAAAAA 
CAGCAGCTGG AAGAGGACCT CAAGGGCTAC CTGGACTGGA TCACCCAGGC AGAAGACATC 
GATCCCGAGA ACGAGGAGGA GGGTGGCGAG GAAGGCAAAC GAAACACTAG CATGCCCACC 
AGCGAGACTG AATCTGTCAA CACAGAGAAC GTAAGTGGTG AAGGCGAGAC CCAGGGATGC 
TGTGGAACTC TCTGGTGCTG GTGGAAAAGA AGAGGCGCGG CCAAGACCGG GCCCTCTGGG 
TGTCGGCGGT GGGGTCAGGC CATCTCGAAA TCCAAACTCA GCCGTCGATG GCGTCGCTGG 
AACCGGTTCA ATCGCAGGAG GTGCAGGGCT GCCGTGAAGT CTGTCACGTT TTACTGGCTG 
GTGATTGTCC TGGTGTTTCT CAACACTTTA ACTATTTCCT CTGAGCATTA CAATCAGCCA 
GACTGGCTGA CACAGATTCA AGACATCGCA AACAAAGTCC TCTTGGCTCT GTTCACCTGT 
GAGATGCTGG TGAAGATGTA CAGCCTGGGC CTCCAGGCTT ACTTTGTCTC CCTCTTCAAC 
CGCTTCGATT GCTTTGTGGT GTGTGGGGGC ATCACCGAAA CCATCCTGGT GGAACTGGAG 
CTCATGTCTC CCCTGGGGGT CTCCGTGTTC CGGTGTGTGC GCCTCCTGAG AATCTTCAAA 
GTGACCAGGC ACTGGACCTC CCTGAGCAAC TTGGTGGCAT CCTTGTTAAA CTCCATGAAG 
TCCATCGCCT CTCTGCTGTT ACTCCTCTTC CTCTTCATCA TCATCTTCTC CTTGCTGGGG 
ATGCAGTTGT TTGGTGGGAA GTTTAATTTT GACGAGACTC AAACCAAGCG AAGCACCTTT 
GACAACTTTC CGCAAGCACT CCTGACGGTG TTCCAGATCC TGACAGGTGA AGACTGGAAT 
GCGGTGATGT ATGACGGCAT CATGGCCTAT GGAGGCCCTT CCTCTTCCGG GATGATTGTC 
TGTATCTACT TCATCATCCT TTTCATCTGT GGTAACTATA TCCTGCTGAA TGTCTTCTTG 
GCCATCGCTG TGGACAATTT GGCTGATGCT GAAAGTCTGA ACACTGCTCA GAAAGAGGAA 
GCTGAAGAAA AAGAAAGGAA AAAGATTGCC AGAAAAGAAA GCCTAGAAAA CAAAAAGAAC 
AACAAACCAG AAGTCAACCA GATAGCCAAC AGTGACAACA AGGTTACAAT TGATGATTAT 
CAGGAAGATG CTGAAGACAA GGACCCTTAC CCGCCCTGTG ATGTGCCAGT AGGTGAAGAG 
GAAGAGGAGG AGGAAGAGGA TGAGCCCGAA GTTCCTGCTG GTCCCCGCCC TCGCAGAATC 
TCAGAGTTGA ACATGAAGGA GAAGATTGCG CCCATCCCAG AAGGAAGCGC CTTCTTCATT 
CTTAGCAAGA CCAACCCGAT ACGTGTGGGT TGCCACAAGC TCATTAACCA CCACATCTTC 
ACCAACCTTA TCCTGGTCTT CATCATGCTG AGCAGCGCTG CCCTGGCTGC AGAGGACCCC 
ATCCGTAGCC ACTCATTCCG CAACACGATA CTGGGCTACT TTGACTATGC CTTCACAGCC 
ATCTTTACGG TTGAAATCCT GTTAAAGATG ACAACTTTTG GAGCCTTCTT GCACAAAGGG 
GCTTTCTGCA GGAACTACTT CAATTTGCTG GACATGCTGG TTGTTGGGGT GTCGCTGGTG 
TCCTTTGGGA TTCAGTCCAG CGCCATCTCC GTTGTGAAGA TTCTGAGGGT TTTGAGGGTC 
TTGCGGCCTC TCAGAGCAAT CAACAGAGCA AAGGGACTTA AGCACGTGGT CCAGTGTGTC 
TTTGTGGCCA TCCGAACCAT TGGCAACATC ATGATCGTCA CGACCCTGCT CCAGTTCATG 
TTTGCATGCA TTGGGGTCCA GCTGTTCAAG GGGAAGTTCT ACCGTTGCAC AGATGAAGCC 
AAAAGTAACC CTGAGGAGTG CAGGGGGCTT TTCATTCTTT ATAAGGACGG CGATGTTGAC 
AGTCCTGTGG TCCGTGAGAG GATCTGGCAA AATAGTGATT TCAATTTTGA CAATGTCCTT 
TCGGCTATGA TGGCGCTGTT CACAGTCTCA ACTTTTGAGG GCTGGCCCGC GTTGCTGTAC 
AAAGCTATTG ATTCAAACGG TGAGAACGTT GGTCCTGTCT ACAACTACCG TGTGGAGATC 
TCCATCTTCT TCATCATCTA CATCATCATC GTTGCCTTCT TCATGATGAA CATCTTTGTG 
GGCTTCGTCA TCGTCACCTT CCAGGAACAG GGAGAAAAAG AGTATAAGAA CTGTGAGCTG 
GACAAAAATC AGCGTCAGTG TGTTGAATAT GCCTTGAAAG CACGCCCCTT AAGGAGATAC 
ATCCCCAAAA ACCCATACCA GTACAAGTTC TGGTATGTGG TGAACTCCTC GCCTTTCGAA 
TACATGATGT TCGTCCTCAT CATGCTCAAC ACGCTCTGCC TGGCCATGCA GCACTATGAG 
CAGTCCAAGA TGTTCAATGA TGCCATGGAC ATTCTGAACA TGGTCTTCAC AGGGGTCTTC 
ACTGTTGAGA TGGTTTTGAA AGTCATTGCT TTCAAGCCCA AGGGGTATTT TAGTGACGCC 
TGGAACACGT TTGACTCCCT CATCGTAATC GGCAGCATTA TAGACGTGGC CCTCAGCGAA 
GCCGACCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171307.1
CDS	287..6706
Translation	MSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315818.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315818.1

ORF Insert Sequence:

ATGAGCACCT CTGCACCCCC ACCTGTAGGA TCTCTCTCCC AAAGAAAACG TCAGCAATAC 
GCCAAGAGCA AAAAACAGGG TAACTCGTCT AACAGCCGAC CTGCCCGTGC CCTTTTCTGT 
TTATCCCTCA ATAACCCCAT CCGAAGAGCC TGCATTAGTA TAGTGGAATG GAAACCATTT 
GACATATTTA TATTATTGGC TATTTTTGCC AATTGTGTGG CCTTAGCTAT TTACATCCCA 
TTCCCTGAAG ATGATTCTAA TTCAACAAAT CATAACTTGG AAAAAGTAGA ATATGCCTTC 
CTGATTATTT TTACAGTGGA GACATTTCTG AAGATTATAG CGTATGGACT GTTGCTGCAT 
CCTAATGCTT ACGTTAGGAA TGGATGGAAT TTACTGGATT TTGTTATAGT AATTGTAGGA 
TTGTTTAGTG TAATTCTGGA ACAATTAACC AAAGAAACAG AAGGCGGGAA CCACTCTAGT 
GGCAAATCAG GAGGCTTTGA TGTCAAAGCC CTCCGTGCCT TCCGAGTGTT AAGACCACTT 
CGACTTGTAT CAGGAGTGCC CAGTTTACAA GTTGTTCTGA ACTCCATTAT AAAAGCCATG 
GTCCCCCTCC TTCACATAGC CCTTTTGGTA TTATTTGTAA TTATCATCTA TGCTATTATA 
GGATTGGAAC TTTTTATTGG AAAAATGCAC AAAACATGTT TTTTTGCTGA CTCAGATATA 
GTAGCTGAAG AGGACCCAGC TCCGTGTGCG TTCTCAGGGA ATGGACGCCA GTGCACCGCC 
AATGGCACGG AATGTAGGAG TGGCTGGGTT GGCCCCAATG GAGGCATCAC TAACTTTGAT 
AACTTTGCCT TTGCCATGCT CACTGTGTTC CAGTGCATTA CCATGGAGGG CTGGACAGAT 
GTGCTCTACT GGGTTAATGA TGCGATAGGA TGGGAATGGC CATGGGTGTA TTTTGTTAGT 
CTGATCATCC TTGGCTCATT TTTCGTCCTT AACCTGGTTC TTGGTGTCCT TAGTGGAGAA 
TTCTCCAAGG AAAGAGAGAA GGCTAAAGCT CGTGGAGATT TCCAGAAGCT TCGGGAAAAA 
CAGCAGCTGG AAGAGGACCT CAAGGGCTAC CTGGACTGGA TCACCCAGGC AGAAGACATC 
GATCCCGAGA ACGAGGAGGA GGGTGGCGAG GAAGGCAAAC GAAACACTAG CATGCCCACC 
AGCGAGACTG AATCTGTCAA CACAGAGAAC GTAAGTGGTG AAGGCGAGAC CCAGGGATGC 
TGTGGAACTC TCTGGTGCTG GTGGAAAAGA AGAGGCGCGG CCAAGACCGG GCCCTCTGGG 
TGTCGGCGGT GGGGTCAGGC CATCTCGAAA TCCAAACTCA GCCGTCGATG GCGTCGCTGG 
AACCGGTTCA ATCGCAGGAG GTGCAGGGCT GCCGTGAAGT CTGTCACGTT TTACTGGCTG 
GTGATTGTCC TGGTGTTTCT CAACACTTTA ACTATTTCCT CTGAGCATTA CAATCAGCCA 
GACTGGCTGA CACAGATTCA AGACATCGCA AACAAAGTCC TCTTGGCTCT GTTCACCTGT 
GAGATGCTGG TGAAGATGTA CAGCCTGGGC CTCCAGGCTT ACTTTGTCTC CCTCTTCAAC 
CGCTTCGATT GCTTTGTGGT GTGTGGGGGC ATCACCGAAA CCATCCTGGT GGAACTGGAG 
CTCATGTCTC CCCTGGGGGT CTCCGTGTTC CGGTGTGTGC GCCTCCTGAG AATCTTCAAA 
GTGACCAGGC ACTGGACCTC CCTGAGCAAC TTGGTGGCAT CCTTGTTAAA CTCCATGAAG 
TCCATCGCCT CTCTGCTGTT ACTCCTCTTC CTCTTCATCA TCATCTTCTC CTTGCTGGGG 
ATGCAGTTGT TTGGTGGGAA GTTTAATTTT GACGAGACTC AAACCAAGCG AAGCACCTTT 
GACAACTTTC CGCAAGCACT CCTGACGGTG TTCCAGATCC TGACAGGTGA AGACTGGAAT 
GCGGTGATGT ATGACGGCAT CATGGCCTAT GGAGGCCCTT CCTCTTCCGG GATGATTGTC 
TGTATCTACT TCATCATCCT TTTCATCTGT GGTAACTATA TCCTGCTGAA TGTCTTCTTG 
GCCATCGCTG TGGACAATTT GGCTGATGCT GAAAGTCTGA ACACTGCTCA GAAAGAGGAA 
GCTGAAGAAA AAGAAAGGAA AAAGATTGCC AGAAAAGAAA GCCTAGAAAA CAAAAAGAAC 
AACAAACCAG AAGTCAACCA GATAGCCAAC AGTGACAACA AGGTTACAAT TGATGATTAT 
CAGGAAGATG CTGAAGACAA GGACCCTTAC CCGCCCTGTG ATGTGCCAGT AGGTGAAGAG 
GAAGAGGAGG AGGAAGAGGA TGAGCCCGAA GTTCCTGCTG GTCCCCGCCC TCGCAGAATC 
TCAGAGTTGA ACATGAAGGA GAAGATTGCG CCCATCCCAG AAGGAAGCGC CTTCTTCATT 
CTTAGCAAGA CCAACCCGAT ACGTGTGGGT TGCCACAAGC TCATTAACCA CCACATCTTC 
ACCAACCTTA TCCTGGTCTT CATCATGCTG AGCAGCGCTG CCCTGGCTGC AGAGGACCCC 
ATCCGTAGCC ACTCATTCCG CAACACGATA CTGGGCTACT TTGACTATGC CTTCACAGCC 
ATCTTTACGG TTGAAATCCT GTTAAAGATG ACAACTTTTG GAGCCTTCTT GCACAAAGGG 
GCTTTCTGCA GGAACTACTT CAATTTGCTG GACATGCTGG TTGTTGGGGT GTCGCTGGTG 
TCCTTTGGGA TTCAGTCCAG CGCCATCTCC GTTGTGAAGA TTCTGAGGGT TTTGAGGGTC 
TTGCGGCCTC TCAGAGCAAT CAACAGAGCA AAGGGACTTA AGCACGTGGT CCAGTGTGTC 
TTTGTGGCCA TCCGAACCAT TGGCAACATC ATGATCGTCA CGACCCTGCT CCAGTTCATG 
TTTGCATGCA TTGGGGTCCA GCTGTTCAAG GGGAAGTTCT ACCGTTGCAC AGATGAAGCC 
AAAAGTAACC CTGAGGAGTG CAGGGGGCTT TTCATTCTTT ATAAGGACGG CGATGTTGAC 
AGTCCTGTGG TCCGTGAGAG GATCTGGCAA AATAGTGATT TCAATTTTGA CAATGTCCTT 
TCGGCTATGA TGGCGCTGTT CACAGTCTCA ACTTTTGAGG GCTGGCCCGC GTTGCTGTAC 
AAAGCTATTG ATTCAAACGG TGAGAACGTT GGTCCTGTCT ACAACTACCG TGTGGAGATC 
TCCATCTTCT TCATCATCTA CATCATCATC GTTGCCTTCT TCATGATGAA CATCTTTGTG 
GGCTTCGTCA TCGTCACCTT CCAGGAACAG GGAGAAAAAG AGTATAAGAA CTGTGAGCTG 
GACAAAAATC AGCGTCAGTG TGTTGAATAT GCCTTGAAAG CACGCCCCTT AAGGAGATAC 
ATCCCCAAAA ACCCATACCA GTACAAGTTC TGGTATGTGG TGAACTCCTC GCCTTTCGAA 
TACATGATGT TCGTCCTCAT CATGCTCAAC ACGCTCTGCC TGGCCATGCA GCACTATGAG 
CAGTCCAAGA TGTTCAATGA TGCCATGGAC ATTCTGAACA TGGTCTTCAC AGGGGTCTTC 
ACTGTTGAGA TGGTTTTGAA AGTCATTGCT TTCAAGCCCA AGGGGTATTT TAGTGACGCC 
TGGAACACGT TTGACTCCCT CATCGTAATC GGCAGCATTA TAGACGTGGC CCTCAGCGAA 
GCCGACCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315818.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171307.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X13, mRNA.

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CloneID	OMu78648
Clone ID Related Accession (Same CDS sequence)	XM_017315818.1 , XM_017315818.2
Accession Version	XM_017315818.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315818.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCT CTGCACCCCC ACCTGTAGGA TCTCTCTCCC AAAGAAAACG TCAGCAATAC 
GCCAAGAGCA AAAAACAGGG TAACTCGTCT AACAGCCGAC CTGCCCGTGC CCTTTTCTGT 
TTATCCCTCA ATAACCCCAT CCGAAGAGCC TGCATTAGTA TAGTGGAATG GAAACCATTT 
GACATATTTA TATTATTGGC TATTTTTGCC AATTGTGTGG CCTTAGCTAT TTACATCCCA 
TTCCCTGAAG ATGATTCTAA TTCAACAAAT CATAACTTGG AAAAAGTAGA ATATGCCTTC 
CTGATTATTT TTACAGTGGA GACATTTCTG AAGATTATAG CGTATGGACT GTTGCTGCAT 
CCTAATGCTT ACGTTAGGAA TGGATGGAAT TTACTGGATT TTGTTATAGT AATTGTAGGA 
TTGTTTAGTG TAATTCTGGA ACAATTAACC AAAGAAACAG AAGGCGGGAA CCACTCTAGT 
GGCAAATCAG GAGGCTTTGA TGTCAAAGCC CTCCGTGCCT TCCGAGTGTT AAGACCACTT 
CGACTTGTAT CAGGAGTGCC CAGTTTACAA GTTGTTCTGA ACTCCATTAT AAAAGCCATG 
GTCCCCCTCC TTCACATAGC CCTTTTGGTA TTATTTGTAA TTATCATCTA TGCTATTATA 
GGATTGGAAC TTTTTATTGG AAAAATGCAC AAAACATGTT TTTTTGCTGA CTCAGATATA 
GTAGCTGAAG AGGACCCAGC TCCGTGTGCG TTCTCAGGGA ATGGACGCCA GTGCACCGCC 
AATGGCACGG AATGTAGGAG TGGCTGGGTT GGCCCCAATG GAGGCATCAC TAACTTTGAT 
AACTTTGCCT TTGCCATGCT CACTGTGTTC CAGTGCATTA CCATGGAGGG CTGGACAGAT 
GTGCTCTACT GGGTTAATGA TGCGATAGGA TGGGAATGGC CATGGGTGTA TTTTGTTAGT 
CTGATCATCC TTGGCTCATT TTTCGTCCTT AACCTGGTTC TTGGTGTCCT TAGTGGAGAA 
TTCTCCAAGG AAAGAGAGAA GGCTAAAGCT CGTGGAGATT TCCAGAAGCT TCGGGAAAAA 
CAGCAGCTGG AAGAGGACCT CAAGGGCTAC CTGGACTGGA TCACCCAGGC AGAAGACATC 
GATCCCGAGA ACGAGGAGGA GGGTGGCGAG GAAGGCAAAC GAAACACTAG CATGCCCACC 
AGCGAGACTG AATCTGTCAA CACAGAGAAC GTAAGTGGTG AAGGCGAGAC CCAGGGATGC 
TGTGGAACTC TCTGGTGCTG GTGGAAAAGA AGAGGCGCGG CCAAGACCGG GCCCTCTGGG 
TGTCGGCGGT GGGGTCAGGC CATCTCGAAA TCCAAACTCA GCCGTCGATG GCGTCGCTGG 
AACCGGTTCA ATCGCAGGAG GTGCAGGGCT GCCGTGAAGT CTGTCACGTT TTACTGGCTG 
GTGATTGTCC TGGTGTTTCT CAACACTTTA ACTATTTCCT CTGAGCATTA CAATCAGCCA 
GACTGGCTGA CACAGATTCA AGACATCGCA AACAAAGTCC TCTTGGCTCT GTTCACCTGT 
GAGATGCTGG TGAAGATGTA CAGCCTGGGC CTCCAGGCTT ACTTTGTCTC CCTCTTCAAC 
CGCTTCGATT GCTTTGTGGT GTGTGGGGGC ATCACCGAAA CCATCCTGGT GGAACTGGAG 
CTCATGTCTC CCCTGGGGGT CTCCGTGTTC CGGTGTGTGC GCCTCCTGAG AATCTTCAAA 
GTGACCAGGC ACTGGACCTC CCTGAGCAAC TTGGTGGCAT CCTTGTTAAA CTCCATGAAG 
TCCATCGCCT CTCTGCTGTT ACTCCTCTTC CTCTTCATCA TCATCTTCTC CTTGCTGGGG 
ATGCAGTTGT TTGGTGGGAA GTTTAATTTT GACGAGACTC AAACCAAGCG AAGCACCTTT 
GACAACTTTC CGCAAGCACT CCTGACGGTG TTCCAGATCC TGACAGGTGA AGACTGGAAT 
GCGGTGATGT ATGACGGCAT CATGGCCTAT GGAGGCCCTT CCTCTTCCGG GATGATTGTC 
TGTATCTACT TCATCATCCT TTTCATCTGT GGTAACTATA TCCTGCTGAA TGTCTTCTTG 
GCCATCGCTG TGGACAATTT GGCTGATGCT GAAAGTCTGA ACACTGCTCA GAAAGAGGAA 
GCTGAAGAAA AAGAAAGGAA AAAGATTGCC AGAAAAGAAA GCCTAGAAAA CAAAAAGAAC 
AACAAACCAG AAGTCAACCA GATAGCCAAC AGTGACAACA AGGTTACAAT TGATGATTAT 
CAGGAAGATG CTGAAGACAA GGACCCTTAC CCGCCCTGTG ATGTGCCAGT AGGTGAAGAG 
GAAGAGGAGG AGGAAGAGGA TGAGCCCGAA GTTCCTGCTG GTCCCCGCCC TCGCAGAATC 
TCAGAGTTGA ACATGAAGGA GAAGATTGCG CCCATCCCAG AAGGAAGCGC CTTCTTCATT 
CTTAGCAAGA CCAACCCGAT ACGTGTGGGT TGCCACAAGC TCATTAACCA CCACATCTTC 
ACCAACCTTA TCCTGGTCTT CATCATGCTG AGCAGCGCTG CCCTGGCTGC AGAGGACCCC 
ATCCGTAGCC ACTCATTCCG CAACACGATA CTGGGCTACT TTGACTATGC CTTCACAGCC 
ATCTTTACGG TTGAAATCCT GTTAAAGATG ACAACTTTTG GAGCCTTCTT GCACAAAGGG 
GCTTTCTGCA GGAACTACTT CAATTTGCTG GACATGCTGG TTGTTGGGGT GTCGCTGGTG 
TCCTTTGGGA TTCAGTCCAG CGCCATCTCC GTTGTGAAGA TTCTGAGGGT TTTGAGGGTC 
TTGCGGCCTC TCAGAGCAAT CAACAGAGCA AAGGGACTTA AGCACGTGGT CCAGTGTGTC 
TTTGTGGCCA TCCGAACCAT TGGCAACATC ATGATCGTCA CGACCCTGCT CCAGTTCATG 
TTTGCATGCA TTGGGGTCCA GCTGTTCAAG GGGAAGTTCT ACCGTTGCAC AGATGAAGCC 
AAAAGTAACC CTGAGGAGTG CAGGGGGCTT TTCATTCTTT ATAAGGACGG CGATGTTGAC 
AGTCCTGTGG TCCGTGAGAG GATCTGGCAA AATAGTGATT TCAATTTTGA CAATGTCCTT 
TCGGCTATGA TGGCGCTGTT CACAGTCTCA ACTTTTGAGG GCTGGCCCGC GTTGCTGTAC 
AAAGCTATTG ATTCAAACGG TGAGAACGTT GGTCCTGTCT ACAACTACCG TGTGGAGATC 
TCCATCTTCT TCATCATCTA CATCATCATC GTTGCCTTCT TCATGATGAA CATCTTTGTG 
GGCTTCGTCA TCGTCACCTT CCAGGAACAG GGAGAAAAAG AGTATAAGAA CTGTGAGCTG 
GACAAAAATC AGCGTCAGTG TGTTGAATAT GCCTTGAAAG CACGCCCCTT AAGGAGATAC 
ATCCCCAAAA ACCCATACCA GTACAAGTTC TGGTATGTGG TGAACTCCTC GCCTTTCGAA 
TACATGATGT TCGTCCTCAT CATGCTCAAC ACGCTCTGCC TGGCCATGCA GCACTATGAG 
CAGTCCAAGA TGTTCAATGA TGCCATGGAC ATTCTGAACA TGGTCTTCAC AGGGGTCTTC 
ACTGTTGAGA TGGTTTTGAA AGTCATTGCT TTCAAGCCCA AGGGGTATTT TAGTGACGCC 
TGGAACACGT TTGACTCCCT CATCGTAATC GGCAGCATTA TAGACGTGGC CCTCAGCGAA 
GCCGACCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171307.1
CDS	285..6704
Translation	MSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315818.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315818.2

ORF Insert Sequence:

ATGAGCACCT CTGCACCCCC ACCTGTAGGA TCTCTCTCCC AAAGAAAACG TCAGCAATAC 
GCCAAGAGCA AAAAACAGGG TAACTCGTCT AACAGCCGAC CTGCCCGTGC CCTTTTCTGT 
TTATCCCTCA ATAACCCCAT CCGAAGAGCC TGCATTAGTA TAGTGGAATG GAAACCATTT 
GACATATTTA TATTATTGGC TATTTTTGCC AATTGTGTGG CCTTAGCTAT TTACATCCCA 
TTCCCTGAAG ATGATTCTAA TTCAACAAAT CATAACTTGG AAAAAGTAGA ATATGCCTTC 
CTGATTATTT TTACAGTGGA GACATTTCTG AAGATTATAG CGTATGGACT GTTGCTGCAT 
CCTAATGCTT ACGTTAGGAA TGGATGGAAT TTACTGGATT TTGTTATAGT AATTGTAGGA 
TTGTTTAGTG TAATTCTGGA ACAATTAACC AAAGAAACAG AAGGCGGGAA CCACTCTAGT 
GGCAAATCAG GAGGCTTTGA TGTCAAAGCC CTCCGTGCCT TCCGAGTGTT AAGACCACTT 
CGACTTGTAT CAGGAGTGCC CAGTTTACAA GTTGTTCTGA ACTCCATTAT AAAAGCCATG 
GTCCCCCTCC TTCACATAGC CCTTTTGGTA TTATTTGTAA TTATCATCTA TGCTATTATA 
GGATTGGAAC TTTTTATTGG AAAAATGCAC AAAACATGTT TTTTTGCTGA CTCAGATATA 
GTAGCTGAAG AGGACCCAGC TCCGTGTGCG TTCTCAGGGA ATGGACGCCA GTGCACCGCC 
AATGGCACGG AATGTAGGAG TGGCTGGGTT GGCCCCAATG GAGGCATCAC TAACTTTGAT 
AACTTTGCCT TTGCCATGCT CACTGTGTTC CAGTGCATTA CCATGGAGGG CTGGACAGAT 
GTGCTCTACT GGGTTAATGA TGCGATAGGA TGGGAATGGC CATGGGTGTA TTTTGTTAGT 
CTGATCATCC TTGGCTCATT TTTCGTCCTT AACCTGGTTC TTGGTGTCCT TAGTGGAGAA 
TTCTCCAAGG AAAGAGAGAA GGCTAAAGCT CGTGGAGATT TCCAGAAGCT TCGGGAAAAA 
CAGCAGCTGG AAGAGGACCT CAAGGGCTAC CTGGACTGGA TCACCCAGGC AGAAGACATC 
GATCCCGAGA ACGAGGAGGA GGGTGGCGAG GAAGGCAAAC GAAACACTAG CATGCCCACC 
AGCGAGACTG AATCTGTCAA CACAGAGAAC GTAAGTGGTG AAGGCGAGAC CCAGGGATGC 
TGTGGAACTC TCTGGTGCTG GTGGAAAAGA AGAGGCGCGG CCAAGACCGG GCCCTCTGGG 
TGTCGGCGGT GGGGTCAGGC CATCTCGAAA TCCAAACTCA GCCGTCGATG GCGTCGCTGG 
AACCGGTTCA ATCGCAGGAG GTGCAGGGCT GCCGTGAAGT CTGTCACGTT TTACTGGCTG 
GTGATTGTCC TGGTGTTTCT CAACACTTTA ACTATTTCCT CTGAGCATTA CAATCAGCCA 
GACTGGCTGA CACAGATTCA AGACATCGCA AACAAAGTCC TCTTGGCTCT GTTCACCTGT 
GAGATGCTGG TGAAGATGTA CAGCCTGGGC CTCCAGGCTT ACTTTGTCTC CCTCTTCAAC 
CGCTTCGATT GCTTTGTGGT GTGTGGGGGC ATCACCGAAA CCATCCTGGT GGAACTGGAG 
CTCATGTCTC CCCTGGGGGT CTCCGTGTTC CGGTGTGTGC GCCTCCTGAG AATCTTCAAA 
GTGACCAGGC ACTGGACCTC CCTGAGCAAC TTGGTGGCAT CCTTGTTAAA CTCCATGAAG 
TCCATCGCCT CTCTGCTGTT ACTCCTCTTC CTCTTCATCA TCATCTTCTC CTTGCTGGGG 
ATGCAGTTGT TTGGTGGGAA GTTTAATTTT GACGAGACTC AAACCAAGCG AAGCACCTTT 
GACAACTTTC CGCAAGCACT CCTGACGGTG TTCCAGATCC TGACAGGTGA AGACTGGAAT 
GCGGTGATGT ATGACGGCAT CATGGCCTAT GGAGGCCCTT CCTCTTCCGG GATGATTGTC 
TGTATCTACT TCATCATCCT TTTCATCTGT GGTAACTATA TCCTGCTGAA TGTCTTCTTG 
GCCATCGCTG TGGACAATTT GGCTGATGCT GAAAGTCTGA ACACTGCTCA GAAAGAGGAA 
GCTGAAGAAA AAGAAAGGAA AAAGATTGCC AGAAAAGAAA GCCTAGAAAA CAAAAAGAAC 
AACAAACCAG AAGTCAACCA GATAGCCAAC AGTGACAACA AGGTTACAAT TGATGATTAT 
CAGGAAGATG CTGAAGACAA GGACCCTTAC CCGCCCTGTG ATGTGCCAGT AGGTGAAGAG 
GAAGAGGAGG AGGAAGAGGA TGAGCCCGAA GTTCCTGCTG GTCCCCGCCC TCGCAGAATC 
TCAGAGTTGA ACATGAAGGA GAAGATTGCG CCCATCCCAG AAGGAAGCGC CTTCTTCATT 
CTTAGCAAGA CCAACCCGAT ACGTGTGGGT TGCCACAAGC TCATTAACCA CCACATCTTC 
ACCAACCTTA TCCTGGTCTT CATCATGCTG AGCAGCGCTG CCCTGGCTGC AGAGGACCCC 
ATCCGTAGCC ACTCATTCCG CAACACGATA CTGGGCTACT TTGACTATGC CTTCACAGCC 
ATCTTTACGG TTGAAATCCT GTTAAAGATG ACAACTTTTG GAGCCTTCTT GCACAAAGGG 
GCTTTCTGCA GGAACTACTT CAATTTGCTG GACATGCTGG TTGTTGGGGT GTCGCTGGTG 
TCCTTTGGGA TTCAGTCCAG CGCCATCTCC GTTGTGAAGA TTCTGAGGGT TTTGAGGGTC 
TTGCGGCCTC TCAGAGCAAT CAACAGAGCA AAGGGACTTA AGCACGTGGT CCAGTGTGTC 
TTTGTGGCCA TCCGAACCAT TGGCAACATC ATGATCGTCA CGACCCTGCT CCAGTTCATG 
TTTGCATGCA TTGGGGTCCA GCTGTTCAAG GGGAAGTTCT ACCGTTGCAC AGATGAAGCC 
AAAAGTAACC CTGAGGAGTG CAGGGGGCTT TTCATTCTTT ATAAGGACGG CGATGTTGAC 
AGTCCTGTGG TCCGTGAGAG GATCTGGCAA AATAGTGATT TCAATTTTGA CAATGTCCTT 
TCGGCTATGA TGGCGCTGTT CACAGTCTCA ACTTTTGAGG GCTGGCCCGC GTTGCTGTAC 
AAAGCTATTG ATTCAAACGG TGAGAACGTT GGTCCTGTCT ACAACTACCG TGTGGAGATC 
TCCATCTTCT TCATCATCTA CATCATCATC GTTGCCTTCT TCATGATGAA CATCTTTGTG 
GGCTTCGTCA TCGTCACCTT CCAGGAACAG GGAGAAAAAG AGTATAAGAA CTGTGAGCTG 
GACAAAAATC AGCGTCAGTG TGTTGAATAT GCCTTGAAAG CACGCCCCTT AAGGAGATAC 
ATCCCCAAAA ACCCATACCA GTACAAGTTC TGGTATGTGG TGAACTCCTC GCCTTTCGAA 
TACATGATGT TCGTCCTCAT CATGCTCAAC ACGCTCTGCC TGGCCATGCA GCACTATGAG 
CAGTCCAAGA TGTTCAATGA TGCCATGGAC ATTCTGAACA TGGTCTTCAC AGGGGTCTTC 
ACTGTTGAGA TGGTTTTGAA AGTCATTGCT TTCAAGCCCA AGGGGTATTT TAGTGACGCC 
TGGAACACGT TTGACTCCCT CATCGTAATC GGCAGCATTA TAGACGTGGC CCTCAGCGAA 
GCCGACCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315819.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171308.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X14, mRNA.

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CloneID	OMu78649
Clone ID Related Accession (Same CDS sequence)	XM_017315819.1 , XM_017315819.2
Accession Version	XM_017315819.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGGTTGG TGAAACTTCT CAGCAGGGGG GAAGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TTCAGGCGCT GCCGTATGTT GCCCTCCTCA TTGCCATGCT CTTCTTCATC 
TACGCTGTCA TAGGCATGCA GATGTTTGGG AAAGTTGCCA TGAGAGATAA CAACCAGATC 
AATAGGAACA ACAACTTCCA GACCTTTCCT CAGGCAGTGC TCCTACTCTT CAGGTGTGCA 
ACAGGGGAAG CCTGGCAGGA GATCATGCTT GCCTGCCTCC CAGGAAAGCT GTGCGACCCA 
GACTCCGATT ACAACCCAGG AGAGGAGTAC ACTTGTGGGA GCAACTTTGC CATTGTCTAC 
TTCATCAGCT TTTACATGCT CTGTGCATTC CTGATCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAATT TTGACTATCT GACTCGGGAC TGGTCTATTC TGGGGCCTCA CCACTTGGAC 
GAATTCAAAA GAATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAACACCTT 
GATGTGGTCA CTCTGCTCCG GCGGATCCAG CCTCCCCTGG GGTTTGGAAA ATTATGCCCA 
CACCGAGTAG CATGTAAGAG ATTGGTTGCC ATGAACATGC CTCTCAACAG TGATGGGACA 
GTCATGTTCA ATGCAACCCT GTTTGCTTTG GTCCGGACAG CTCTCAAGAT CAAGACTGAA 
GGGAACCTGG AGCAAGCTAA TGAAGAACTC CGAGCTGTGA TCAAGAAAAT CTGGAAGAAG 
ACAAGCATGA AGCTGCTTGA CCAAGTTGTC CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAGCAAGGAC TGGTGGGGAA GTACCCTGCG AAGAACACCA CGATTGCCCT ACAGGCGGGA 
TTAAGGACCC TGCATGACAT TGGGCCAGAA ATCCGACGGG CTATATCCTG TGACTTGCAA 
GATGACGAGC CAGAAGACTC CAAACCAGAA GAAGAAGATG TATTCAAAAG AAATGGTGCC 
CTGCTTGGAA ACCATGTCAA TCATGTTAAT AGTGATAGGA GAGATTCCCT TCAGCAGACC 
AATACCACCC ACCGTCCCCT GCATGTCCAA AGGCCTTCAA TGCCACCTGC AAGTGATACT 
GAGAAACCGC TGTTTCCTCC AGCAGGAAAT TCGGGGTGTC ATAACCATCA TAACCATAAT 
TCCATAGGGA AGCAAGCTCC CACCTCAACA AATGCCAATC TCAATAATGC CAATATGTCC 
AAAGCTGCCC ACGGAAAGCC GCCCAGCATT GGGAACCTTG AGCATGTGTC TGAAAATGGG 
CATTACTCCT GCAAGCATGA TCGAGAGCTT CAAAGAAGGT CCAGTATTAA AAGAACCCGC 
TATTACGAGA CTTACATTAG GTCTGAGTCA GGAGATGAGC AATTTCCAAC CATTTGCCGT 
GAAGATCCTG AGATACACGG CTACTTCCGA GACCCCCGCT GCTTGGGGGA ACAGGAGTAT 
TTCAGTAGCG AGGAGTGCTG TGAGGACGAC AGCTCTCCCA CCTGGAGCAG GCAAAACTAC 
AACTACTACA ACAGGTACCC AGGCAGCTCC ATGGACTTTG AGAGGCCCCG AGGCTACCAC 
CACCCCCAAG GCTTCTTAGA AGATGATGAC TCTCCCACTG GCTATGATTC ACGGAGATCT 
CCGAGGAGAC GTCTTCTACC TCCTACCCCA CCATCCCATC GGAGGTCCTC CTTCAACTTT 
GAGTGTCTGC GCAGGCAAAG CAGCCAAGAC GATGTCCTTC CATCGCCTGC CCTGCCTCAC 
CGCGCTGCCC TGCCCCTGCA CCTGATGCAG CAGCAGATCA TGGCCGTTGC AGGCCTAGAT 
TCCAGTAAAG CCCAGAAGTA CTCACCGAGC CACTCTACCC GGTCGTGGGC CACCCCTCCA 
GCAACCCCTC CATACCGAGA CTGGAGCCCA TGCTACACCC CTTTGATCCA GGTGGACCGC 
TCAGAATCTA TGGACCAGGT CAATGGCAGC CTGCCATCCC TACACCGAAG CTCCTGGTAC 
ACAGATGAAC CCGACATCTC CTATAGGACT TTCACGCCAG CTAGTCTGAC TGTCCCCAGC 
AGCTTCCGGA ACAAGAACAG CGACAAGCAG AGGAGTGCAG ACAGCCTGGT GGAGGCAGTC 
CTGATATCCG AAGGCTTGGG ACGCTACGCG AGGGACCCCA AATTTGTCTC AGCGACAAAA 
CATGAGATTG CCGATGCCTG TGACCTAACC ATAGACGAGA TGGAAAGTGC TGCCAGCACC 
CTGCTCAATG GCAGTGTGTG TCCTCGAGCC AATGGGGACA TGGGCCCCAT CTCCCACCGC 
CAGGACTATG AGCTCCAGGA CTTTGGTCCT GGCTACAGTG ACGAGGAGCC AGACCCTGGG 
CGAGAGGAAG AGGACCTGGC AGATGAGATG ATTTGCATTA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171308.1
CDS	561..6971
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315819.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315819.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGGTTGG TGAAACTTCT CAGCAGGGGG GAAGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TTCAGGCGCT GCCGTATGTT GCCCTCCTCA TTGCCATGCT CTTCTTCATC 
TACGCTGTCA TAGGCATGCA GATGTTTGGG AAAGTTGCCA TGAGAGATAA CAACCAGATC 
AATAGGAACA ACAACTTCCA GACCTTTCCT CAGGCAGTGC TCCTACTCTT CAGGTGTGCA 
ACAGGGGAAG CCTGGCAGGA GATCATGCTT GCCTGCCTCC CAGGAAAGCT GTGCGACCCA 
GACTCCGATT ACAACCCAGG AGAGGAGTAC ACTTGTGGGA GCAACTTTGC CATTGTCTAC 
TTCATCAGCT TTTACATGCT CTGTGCATTC CTGATCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAATT TTGACTATCT GACTCGGGAC TGGTCTATTC TGGGGCCTCA CCACTTGGAC 
GAATTCAAAA GAATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAACACCTT 
GATGTGGTCA CTCTGCTCCG GCGGATCCAG CCTCCCCTGG GGTTTGGAAA ATTATGCCCA 
CACCGAGTAG CATGTAAGAG ATTGGTTGCC ATGAACATGC CTCTCAACAG TGATGGGACA 
GTCATGTTCA ATGCAACCCT GTTTGCTTTG GTCCGGACAG CTCTCAAGAT CAAGACTGAA 
GGGAACCTGG AGCAAGCTAA TGAAGAACTC CGAGCTGTGA TCAAGAAAAT CTGGAAGAAG 
ACAAGCATGA AGCTGCTTGA CCAAGTTGTC CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAGCAAGGAC TGGTGGGGAA GTACCCTGCG AAGAACACCA CGATTGCCCT ACAGGCGGGA 
TTAAGGACCC TGCATGACAT TGGGCCAGAA ATCCGACGGG CTATATCCTG TGACTTGCAA 
GATGACGAGC CAGAAGACTC CAAACCAGAA GAAGAAGATG TATTCAAAAG AAATGGTGCC 
CTGCTTGGAA ACCATGTCAA TCATGTTAAT AGTGATAGGA GAGATTCCCT TCAGCAGACC 
AATACCACCC ACCGTCCCCT GCATGTCCAA AGGCCTTCAA TGCCACCTGC AAGTGATACT 
GAGAAACCGC TGTTTCCTCC AGCAGGAAAT TCGGGGTGTC ATAACCATCA TAACCATAAT 
TCCATAGGGA AGCAAGCTCC CACCTCAACA AATGCCAATC TCAATAATGC CAATATGTCC 
AAAGCTGCCC ACGGAAAGCC GCCCAGCATT GGGAACCTTG AGCATGTGTC TGAAAATGGG 
CATTACTCCT GCAAGCATGA TCGAGAGCTT CAAAGAAGGT CCAGTATTAA AAGAACCCGC 
TATTACGAGA CTTACATTAG GTCTGAGTCA GGAGATGAGC AATTTCCAAC CATTTGCCGT 
GAAGATCCTG AGATACACGG CTACTTCCGA GACCCCCGCT GCTTGGGGGA ACAGGAGTAT 
TTCAGTAGCG AGGAGTGCTG TGAGGACGAC AGCTCTCCCA CCTGGAGCAG GCAAAACTAC 
AACTACTACA ACAGGTACCC AGGCAGCTCC ATGGACTTTG AGAGGCCCCG AGGCTACCAC 
CACCCCCAAG GCTTCTTAGA AGATGATGAC TCTCCCACTG GCTATGATTC ACGGAGATCT 
CCGAGGAGAC GTCTTCTACC TCCTACCCCA CCATCCCATC GGAGGTCCTC CTTCAACTTT 
GAGTGTCTGC GCAGGCAAAG CAGCCAAGAC GATGTCCTTC CATCGCCTGC CCTGCCTCAC 
CGCGCTGCCC TGCCCCTGCA CCTGATGCAG CAGCAGATCA TGGCCGTTGC AGGCCTAGAT 
TCCAGTAAAG CCCAGAAGTA CTCACCGAGC CACTCTACCC GGTCGTGGGC CACCCCTCCA 
GCAACCCCTC CATACCGAGA CTGGAGCCCA TGCTACACCC CTTTGATCCA GGTGGACCGC 
TCAGAATCTA TGGACCAGGT CAATGGCAGC CTGCCATCCC TACACCGAAG CTCCTGGTAC 
ACAGATGAAC CCGACATCTC CTATAGGACT TTCACGCCAG CTAGTCTGAC TGTCCCCAGC 
AGCTTCCGGA ACAAGAACAG CGACAAGCAG AGGAGTGCAG ACAGCCTGGT GGAGGCAGTC 
CTGATATCCG AAGGCTTGGG ACGCTACGCG AGGGACCCCA AATTTGTCTC AGCGACAAAA 
CATGAGATTG CCGATGCCTG TGACCTAACC ATAGACGAGA TGGAAAGTGC TGCCAGCACC 
CTGCTCAATG GCAGTGTGTG TCCTCGAGCC AATGGGGACA TGGGCCCCAT CTCCCACCGC 
CAGGACTATG AGCTCCAGGA CTTTGGTCCT GGCTACAGTG ACGAGGAGCC AGACCCTGGG 
CGAGAGGAAG AGGACCTGGC AGATGAGATG ATTTGCATTA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315819.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171308.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X14, mRNA.

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CloneID	OMu78649
Clone ID Related Accession (Same CDS sequence)	XM_017315819.1 , XM_017315819.2
Accession Version	XM_017315819.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315819.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGGTTGG TGAAACTTCT CAGCAGGGGG GAAGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TTCAGGCGCT GCCGTATGTT GCCCTCCTCA TTGCCATGCT CTTCTTCATC 
TACGCTGTCA TAGGCATGCA GATGTTTGGG AAAGTTGCCA TGAGAGATAA CAACCAGATC 
AATAGGAACA ACAACTTCCA GACCTTTCCT CAGGCAGTGC TCCTACTCTT CAGGTGTGCA 
ACAGGGGAAG CCTGGCAGGA GATCATGCTT GCCTGCCTCC CAGGAAAGCT GTGCGACCCA 
GACTCCGATT ACAACCCAGG AGAGGAGTAC ACTTGTGGGA GCAACTTTGC CATTGTCTAC 
TTCATCAGCT TTTACATGCT CTGTGCATTC CTGATCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAATT TTGACTATCT GACTCGGGAC TGGTCTATTC TGGGGCCTCA CCACTTGGAC 
GAATTCAAAA GAATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAACACCTT 
GATGTGGTCA CTCTGCTCCG GCGGATCCAG CCTCCCCTGG GGTTTGGAAA ATTATGCCCA 
CACCGAGTAG CATGTAAGAG ATTGGTTGCC ATGAACATGC CTCTCAACAG TGATGGGACA 
GTCATGTTCA ATGCAACCCT GTTTGCTTTG GTCCGGACAG CTCTCAAGAT CAAGACTGAA 
GGGAACCTGG AGCAAGCTAA TGAAGAACTC CGAGCTGTGA TCAAGAAAAT CTGGAAGAAG 
ACAAGCATGA AGCTGCTTGA CCAAGTTGTC CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAGCAAGGAC TGGTGGGGAA GTACCCTGCG AAGAACACCA CGATTGCCCT ACAGGCGGGA 
TTAAGGACCC TGCATGACAT TGGGCCAGAA ATCCGACGGG CTATATCCTG TGACTTGCAA 
GATGACGAGC CAGAAGACTC CAAACCAGAA GAAGAAGATG TATTCAAAAG AAATGGTGCC 
CTGCTTGGAA ACCATGTCAA TCATGTTAAT AGTGATAGGA GAGATTCCCT TCAGCAGACC 
AATACCACCC ACCGTCCCCT GCATGTCCAA AGGCCTTCAA TGCCACCTGC AAGTGATACT 
GAGAAACCGC TGTTTCCTCC AGCAGGAAAT TCGGGGTGTC ATAACCATCA TAACCATAAT 
TCCATAGGGA AGCAAGCTCC CACCTCAACA AATGCCAATC TCAATAATGC CAATATGTCC 
AAAGCTGCCC ACGGAAAGCC GCCCAGCATT GGGAACCTTG AGCATGTGTC TGAAAATGGG 
CATTACTCCT GCAAGCATGA TCGAGAGCTT CAAAGAAGGT CCAGTATTAA AAGAACCCGC 
TATTACGAGA CTTACATTAG GTCTGAGTCA GGAGATGAGC AATTTCCAAC CATTTGCCGT 
GAAGATCCTG AGATACACGG CTACTTCCGA GACCCCCGCT GCTTGGGGGA ACAGGAGTAT 
TTCAGTAGCG AGGAGTGCTG TGAGGACGAC AGCTCTCCCA CCTGGAGCAG GCAAAACTAC 
AACTACTACA ACAGGTACCC AGGCAGCTCC ATGGACTTTG AGAGGCCCCG AGGCTACCAC 
CACCCCCAAG GCTTCTTAGA AGATGATGAC TCTCCCACTG GCTATGATTC ACGGAGATCT 
CCGAGGAGAC GTCTTCTACC TCCTACCCCA CCATCCCATC GGAGGTCCTC CTTCAACTTT 
GAGTGTCTGC GCAGGCAAAG CAGCCAAGAC GATGTCCTTC CATCGCCTGC CCTGCCTCAC 
CGCGCTGCCC TGCCCCTGCA CCTGATGCAG CAGCAGATCA TGGCCGTTGC AGGCCTAGAT 
TCCAGTAAAG CCCAGAAGTA CTCACCGAGC CACTCTACCC GGTCGTGGGC CACCCCTCCA 
GCAACCCCTC CATACCGAGA CTGGAGCCCA TGCTACACCC CTTTGATCCA GGTGGACCGC 
TCAGAATCTA TGGACCAGGT CAATGGCAGC CTGCCATCCC TACACCGAAG CTCCTGGTAC 
ACAGATGAAC CCGACATCTC CTATAGGACT TTCACGCCAG CTAGTCTGAC TGTCCCCAGC 
AGCTTCCGGA ACAAGAACAG CGACAAGCAG AGGAGTGCAG ACAGCCTGGT GGAGGCAGTC 
CTGATATCCG AAGGCTTGGG ACGCTACGCG AGGGACCCCA AATTTGTCTC AGCGACAAAA 
CATGAGATTG CCGATGCCTG TGACCTAACC ATAGACGAGA TGGAAAGTGC TGCCAGCACC 
CTGCTCAATG GCAGTGTGTG TCCTCGAGCC AATGGGGACA TGGGCCCCAT CTCCCACCGC 
CAGGACTATG AGCTCCAGGA CTTTGGTCCT GGCTACAGTG ACGAGGAGCC AGACCCTGGG 
CGAGAGGAAG AGGACCTGGC AGATGAGATG ATTTGCATTA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171308.1
CDS	494..6904
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315819.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315819.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGGTTGG TGAAACTTCT CAGCAGGGGG GAAGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TTCAGGCGCT GCCGTATGTT GCCCTCCTCA TTGCCATGCT CTTCTTCATC 
TACGCTGTCA TAGGCATGCA GATGTTTGGG AAAGTTGCCA TGAGAGATAA CAACCAGATC 
AATAGGAACA ACAACTTCCA GACCTTTCCT CAGGCAGTGC TCCTACTCTT CAGGTGTGCA 
ACAGGGGAAG CCTGGCAGGA GATCATGCTT GCCTGCCTCC CAGGAAAGCT GTGCGACCCA 
GACTCCGATT ACAACCCAGG AGAGGAGTAC ACTTGTGGGA GCAACTTTGC CATTGTCTAC 
TTCATCAGCT TTTACATGCT CTGTGCATTC CTGATCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAATT TTGACTATCT GACTCGGGAC TGGTCTATTC TGGGGCCTCA CCACTTGGAC 
GAATTCAAAA GAATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAACACCTT 
GATGTGGTCA CTCTGCTCCG GCGGATCCAG CCTCCCCTGG GGTTTGGAAA ATTATGCCCA 
CACCGAGTAG CATGTAAGAG ATTGGTTGCC ATGAACATGC CTCTCAACAG TGATGGGACA 
GTCATGTTCA ATGCAACCCT GTTTGCTTTG GTCCGGACAG CTCTCAAGAT CAAGACTGAA 
GGGAACCTGG AGCAAGCTAA TGAAGAACTC CGAGCTGTGA TCAAGAAAAT CTGGAAGAAG 
ACAAGCATGA AGCTGCTTGA CCAAGTTGTC CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAGCAAGGAC TGGTGGGGAA GTACCCTGCG AAGAACACCA CGATTGCCCT ACAGGCGGGA 
TTAAGGACCC TGCATGACAT TGGGCCAGAA ATCCGACGGG CTATATCCTG TGACTTGCAA 
GATGACGAGC CAGAAGACTC CAAACCAGAA GAAGAAGATG TATTCAAAAG AAATGGTGCC 
CTGCTTGGAA ACCATGTCAA TCATGTTAAT AGTGATAGGA GAGATTCCCT TCAGCAGACC 
AATACCACCC ACCGTCCCCT GCATGTCCAA AGGCCTTCAA TGCCACCTGC AAGTGATACT 
GAGAAACCGC TGTTTCCTCC AGCAGGAAAT TCGGGGTGTC ATAACCATCA TAACCATAAT 
TCCATAGGGA AGCAAGCTCC CACCTCAACA AATGCCAATC TCAATAATGC CAATATGTCC 
AAAGCTGCCC ACGGAAAGCC GCCCAGCATT GGGAACCTTG AGCATGTGTC TGAAAATGGG 
CATTACTCCT GCAAGCATGA TCGAGAGCTT CAAAGAAGGT CCAGTATTAA AAGAACCCGC 
TATTACGAGA CTTACATTAG GTCTGAGTCA GGAGATGAGC AATTTCCAAC CATTTGCCGT 
GAAGATCCTG AGATACACGG CTACTTCCGA GACCCCCGCT GCTTGGGGGA ACAGGAGTAT 
TTCAGTAGCG AGGAGTGCTG TGAGGACGAC AGCTCTCCCA CCTGGAGCAG GCAAAACTAC 
AACTACTACA ACAGGTACCC AGGCAGCTCC ATGGACTTTG AGAGGCCCCG AGGCTACCAC 
CACCCCCAAG GCTTCTTAGA AGATGATGAC TCTCCCACTG GCTATGATTC ACGGAGATCT 
CCGAGGAGAC GTCTTCTACC TCCTACCCCA CCATCCCATC GGAGGTCCTC CTTCAACTTT 
GAGTGTCTGC GCAGGCAAAG CAGCCAAGAC GATGTCCTTC CATCGCCTGC CCTGCCTCAC 
CGCGCTGCCC TGCCCCTGCA CCTGATGCAG CAGCAGATCA TGGCCGTTGC AGGCCTAGAT 
TCCAGTAAAG CCCAGAAGTA CTCACCGAGC CACTCTACCC GGTCGTGGGC CACCCCTCCA 
GCAACCCCTC CATACCGAGA CTGGAGCCCA TGCTACACCC CTTTGATCCA GGTGGACCGC 
TCAGAATCTA TGGACCAGGT CAATGGCAGC CTGCCATCCC TACACCGAAG CTCCTGGTAC 
ACAGATGAAC CCGACATCTC CTATAGGACT TTCACGCCAG CTAGTCTGAC TGTCCCCAGC 
AGCTTCCGGA ACAAGAACAG CGACAAGCAG AGGAGTGCAG ACAGCCTGGT GGAGGCAGTC 
CTGATATCCG AAGGCTTGGG ACGCTACGCG AGGGACCCCA AATTTGTCTC AGCGACAAAA 
CATGAGATTG CCGATGCCTG TGACCTAACC ATAGACGAGA TGGAAAGTGC TGCCAGCACC 
CTGCTCAATG GCAGTGTGTG TCCTCGAGCC AATGGGGACA TGGGCCCCAT CTCCCACCGC 
CAGGACTATG AGCTCCAGGA CTTTGGTCCT GGCTACAGTG ACGAGGAGCC AGACCCTGGG 
CGAGAGGAAG AGGACCTGGC AGATGAGATG ATTTGCATTA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315823.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171312.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X20, mRNA.

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CloneID	OMu78653
Clone ID Related Accession (Same CDS sequence)	XM_017315823.1 , XM_017315823.2
Accession Version	XM_017315823.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAACTTCTCA GCAGGGGGGA AGGCATCCGG ACACTGCTGT GGACCTTCAT CAAGTCCTTT 
CAGGCGCTGC CGTATGTTGC CCTCCTCATT GCCATGCTCT TCTTCATCTA CGCTGTCATA 
GGCATGCAGA TGTTTGGGAA AGTTGCCATG AGAGATAACA ACCAGATCAA TAGGAACAAC 
AACTTCCAGA CCTTTCCTCA GGCAGTGCTC CTACTCTTCA GGTGTGCAAC AGGGGAAGCC 
TGGCAGGAGA TCATGCTTGC CTGCCTCCCA GGAAAGCTGT GCGACCCAGA CTCCGATTAC 
AACCCAGGAG AGGAGTACAC TTGTGGGAGC AACTTTGCCA TTGTCTACTT CATCAGCTTT 
TACATGCTCT GTGCATTCCT GATCATCAAC CTCTTTGTGG CTGTCATCAT GGACAATTTT 
GACTATCTGA CTCGGGACTG GTCTATTCTG GGGCCTCACC ACTTGGACGA ATTCAAAAGA 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AACACCTTGA TGTGGTCACT 
CTGCTCCGGC GGATCCAGCC TCCCCTGGGG TTTGGAAAAT TATGCCCACA CCGAGTAGCA 
TGTAAGAGAT TGGTTGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTCAAT 
GCAACCCTGT TTGCTTTGGT CCGGACAGCT CTCAAGATCA AGACTGAAGG GAACCTGGAG 
CAAGCTAATG AAGAACTCCG AGCTGTGATC AAGAAAATCT GGAAGAAGAC AAGCATGAAG 
CTGCTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA GCAAGGACTG 
GTGGGGAAGT ACCCTGCGAA GAACACCACG ATTGCCCTAC AGGCGGGATT AAGGACCCTG 
CATGACATTG GGCCAGAAAT CCGACGGGCT ATATCCTGTG ACTTGCAAGA TGACGAGCCA 
GAAGACTCCA AACCAGAAGA AGAAGATGTA TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC 
CATGTCAATC ATGTTAATAG TGATAGGAGA GATTCCCTTC AGCAGACCAA TACCACCCAC 
CGTCCCCTGC ATGTCCAAAG GCCTTCAATG CCACCTGCAA GTGATACTGA GAAACCGCTG 
TTTCCTCCAG CAGGAAATTC GGGGTGTCAT AACCATCATA ACCATAATTC CATAGGGAAG 
CAAGCTCCCA CCTCAACAAA TGCCAATCTC AATAATGCCA ATATGTCCAA AGCTGCCCAC 
GGAAAGCCGC CCAGCATTGG GAACCTTGAG CATGTGTCTG AAAATGGGCA TTACTCCTGC 
AAGCATGATC GAGAGCTTCA AAGAAGGTCC AGTATTAAAA GAACCCGCTA TTACGAGACT 
TACATTAGGT CTGAGTCAGG AGATGAGCAA TTTCCAACCA TTTGCCGTGA AGATCCTGAG 
ATACACGGCT ACTTCCGAGA CCCCCGCTGC TTGGGGGAAC AGGAGTATTT CAGTAGCGAG 
GAGTGCTGTG AGGACGACAG CTCTCCCACC TGGAGCAGGC AAAACTACAA CTACTACAAC 
AGGTACCCAG GCAGCTCCAT GGACTTTGAG AGGCCCCGAG GCTACCACCA CCCCCAAGGC 
TTCTTAGAAG ATGATGACTC TCCCACTGGC TATGATTCAC GGAGATCTCC GAGGAGACGT 
CTTCTACCTC CTACCCCACC ATCCCATCGG AGGTCCTCCT TCAACTTTGA GTGTCTGCGC 
AGGCAAAGCA GCCAAGACGA TGTCCTTCCA TCGCCTGCCC TGCCTCACCG CGCTGCCCTG 
CCCCTGCACC TGATGCAGCA GCAGATCATG GCCGTTGCAG GCCTAGATTC CAGTAAAGCC 
CAGAAGTACT CACCGAGCCA CTCTACCCGG TCGTGGGCCA CCCCTCCAGC AACCCCTCCA 
TACCGAGACT GGAGCCCATG CTACACCCCT TTGATCCAGG TGGACCGCTC AGAATCTATG 
GACCAGGTCA ATGGCAGCCT GCCATCCCTA CACCGAAGCT CCTGGTACAC AGATGAACCC 
GACATCTCCT ATAGGACTTT CACGCCAGCT AGTCTGACTG TCCCCAGCAG CTTCCGGAAC 
AAGAACAGCG ACAAGCAGAG GAGTGCAGAC AGCCTGGTGG AGGCAGTCCT GATATCCGAA 
GGCTTGGGAC GCTACGCGAG GGACCCCAAA TTTGTCTCAG CGACAAAACA TGAGATTGCC 
GATGCCTGTG ACCTAACCAT AGACGAGATG GAAAGTGCTG CCAGCACCCT GCTCAATGGC 
AGTGTGTGTC CTCGAGCCAA TGGGGACATG GGCCCCATCT CCCACCGCCA GGACTATGAG 
CTCCAGGACT TTGGTCCTGG CTACAGTGAC GAGGAGCCAG ACCCTGGGCG AGAGGAAGAG 
GACCTGGCAG ATGAGATGAT TTGCATTACC ACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171312.1
CDS	561..7079
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315823.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315823.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAACTTCTCA GCAGGGGGGA AGGCATCCGG ACACTGCTGT GGACCTTCAT CAAGTCCTTT 
CAGGCGCTGC CGTATGTTGC CCTCCTCATT GCCATGCTCT TCTTCATCTA CGCTGTCATA 
GGCATGCAGA TGTTTGGGAA AGTTGCCATG AGAGATAACA ACCAGATCAA TAGGAACAAC 
AACTTCCAGA CCTTTCCTCA GGCAGTGCTC CTACTCTTCA GGTGTGCAAC AGGGGAAGCC 
TGGCAGGAGA TCATGCTTGC CTGCCTCCCA GGAAAGCTGT GCGACCCAGA CTCCGATTAC 
AACCCAGGAG AGGAGTACAC TTGTGGGAGC AACTTTGCCA TTGTCTACTT CATCAGCTTT 
TACATGCTCT GTGCATTCCT GATCATCAAC CTCTTTGTGG CTGTCATCAT GGACAATTTT 
GACTATCTGA CTCGGGACTG GTCTATTCTG GGGCCTCACC ACTTGGACGA ATTCAAAAGA 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AACACCTTGA TGTGGTCACT 
CTGCTCCGGC GGATCCAGCC TCCCCTGGGG TTTGGAAAAT TATGCCCACA CCGAGTAGCA 
TGTAAGAGAT TGGTTGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTCAAT 
GCAACCCTGT TTGCTTTGGT CCGGACAGCT CTCAAGATCA AGACTGAAGG GAACCTGGAG 
CAAGCTAATG AAGAACTCCG AGCTGTGATC AAGAAAATCT GGAAGAAGAC AAGCATGAAG 
CTGCTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA GCAAGGACTG 
GTGGGGAAGT ACCCTGCGAA GAACACCACG ATTGCCCTAC AGGCGGGATT AAGGACCCTG 
CATGACATTG GGCCAGAAAT CCGACGGGCT ATATCCTGTG ACTTGCAAGA TGACGAGCCA 
GAAGACTCCA AACCAGAAGA AGAAGATGTA TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC 
CATGTCAATC ATGTTAATAG TGATAGGAGA GATTCCCTTC AGCAGACCAA TACCACCCAC 
CGTCCCCTGC ATGTCCAAAG GCCTTCAATG CCACCTGCAA GTGATACTGA GAAACCGCTG 
TTTCCTCCAG CAGGAAATTC GGGGTGTCAT AACCATCATA ACCATAATTC CATAGGGAAG 
CAAGCTCCCA CCTCAACAAA TGCCAATCTC AATAATGCCA ATATGTCCAA AGCTGCCCAC 
GGAAAGCCGC CCAGCATTGG GAACCTTGAG CATGTGTCTG AAAATGGGCA TTACTCCTGC 
AAGCATGATC GAGAGCTTCA AAGAAGGTCC AGTATTAAAA GAACCCGCTA TTACGAGACT 
TACATTAGGT CTGAGTCAGG AGATGAGCAA TTTCCAACCA TTTGCCGTGA AGATCCTGAG 
ATACACGGCT ACTTCCGAGA CCCCCGCTGC TTGGGGGAAC AGGAGTATTT CAGTAGCGAG 
GAGTGCTGTG AGGACGACAG CTCTCCCACC TGGAGCAGGC AAAACTACAA CTACTACAAC 
AGGTACCCAG GCAGCTCCAT GGACTTTGAG AGGCCCCGAG GCTACCACCA CCCCCAAGGC 
TTCTTAGAAG ATGATGACTC TCCCACTGGC TATGATTCAC GGAGATCTCC GAGGAGACGT 
CTTCTACCTC CTACCCCACC ATCCCATCGG AGGTCCTCCT TCAACTTTGA GTGTCTGCGC 
AGGCAAAGCA GCCAAGACGA TGTCCTTCCA TCGCCTGCCC TGCCTCACCG CGCTGCCCTG 
CCCCTGCACC TGATGCAGCA GCAGATCATG GCCGTTGCAG GCCTAGATTC CAGTAAAGCC 
CAGAAGTACT CACCGAGCCA CTCTACCCGG TCGTGGGCCA CCCCTCCAGC AACCCCTCCA 
TACCGAGACT GGAGCCCATG CTACACCCCT TTGATCCAGG TGGACCGCTC AGAATCTATG 
GACCAGGTCA ATGGCAGCCT GCCATCCCTA CACCGAAGCT CCTGGTACAC AGATGAACCC 
GACATCTCCT ATAGGACTTT CACGCCAGCT AGTCTGACTG TCCCCAGCAG CTTCCGGAAC 
AAGAACAGCG ACAAGCAGAG GAGTGCAGAC AGCCTGGTGG AGGCAGTCCT GATATCCGAA 
GGCTTGGGAC GCTACGCGAG GGACCCCAAA TTTGTCTCAG CGACAAAACA TGAGATTGCC 
GATGCCTGTG ACCTAACCAT AGACGAGATG GAAAGTGCTG CCAGCACCCT GCTCAATGGC 
AGTGTGTGTC CTCGAGCCAA TGGGGACATG GGCCCCATCT CCCACCGCCA GGACTATGAG 
CTCCAGGACT TTGGTCCTGG CTACAGTGAC GAGGAGCCAG ACCCTGGGCG AGAGGAAGAG 
GACCTGGCAG ATGAGATGAT TTGCATTACC ACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315823.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171312.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X20, mRNA.

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CloneID	OMu78653
Clone ID Related Accession (Same CDS sequence)	XM_017315823.1 , XM_017315823.2
Accession Version	XM_017315823.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315823.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAACTTCTCA GCAGGGGGGA AGGCATCCGG ACACTGCTGT GGACCTTCAT CAAGTCCTTT 
CAGGCGCTGC CGTATGTTGC CCTCCTCATT GCCATGCTCT TCTTCATCTA CGCTGTCATA 
GGCATGCAGA TGTTTGGGAA AGTTGCCATG AGAGATAACA ACCAGATCAA TAGGAACAAC 
AACTTCCAGA CCTTTCCTCA GGCAGTGCTC CTACTCTTCA GGTGTGCAAC AGGGGAAGCC 
TGGCAGGAGA TCATGCTTGC CTGCCTCCCA GGAAAGCTGT GCGACCCAGA CTCCGATTAC 
AACCCAGGAG AGGAGTACAC TTGTGGGAGC AACTTTGCCA TTGTCTACTT CATCAGCTTT 
TACATGCTCT GTGCATTCCT GATCATCAAC CTCTTTGTGG CTGTCATCAT GGACAATTTT 
GACTATCTGA CTCGGGACTG GTCTATTCTG GGGCCTCACC ACTTGGACGA ATTCAAAAGA 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AACACCTTGA TGTGGTCACT 
CTGCTCCGGC GGATCCAGCC TCCCCTGGGG TTTGGAAAAT TATGCCCACA CCGAGTAGCA 
TGTAAGAGAT TGGTTGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTCAAT 
GCAACCCTGT TTGCTTTGGT CCGGACAGCT CTCAAGATCA AGACTGAAGG GAACCTGGAG 
CAAGCTAATG AAGAACTCCG AGCTGTGATC AAGAAAATCT GGAAGAAGAC AAGCATGAAG 
CTGCTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA GCAAGGACTG 
GTGGGGAAGT ACCCTGCGAA GAACACCACG ATTGCCCTAC AGGCGGGATT AAGGACCCTG 
CATGACATTG GGCCAGAAAT CCGACGGGCT ATATCCTGTG ACTTGCAAGA TGACGAGCCA 
GAAGACTCCA AACCAGAAGA AGAAGATGTA TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC 
CATGTCAATC ATGTTAATAG TGATAGGAGA GATTCCCTTC AGCAGACCAA TACCACCCAC 
CGTCCCCTGC ATGTCCAAAG GCCTTCAATG CCACCTGCAA GTGATACTGA GAAACCGCTG 
TTTCCTCCAG CAGGAAATTC GGGGTGTCAT AACCATCATA ACCATAATTC CATAGGGAAG 
CAAGCTCCCA CCTCAACAAA TGCCAATCTC AATAATGCCA ATATGTCCAA AGCTGCCCAC 
GGAAAGCCGC CCAGCATTGG GAACCTTGAG CATGTGTCTG AAAATGGGCA TTACTCCTGC 
AAGCATGATC GAGAGCTTCA AAGAAGGTCC AGTATTAAAA GAACCCGCTA TTACGAGACT 
TACATTAGGT CTGAGTCAGG AGATGAGCAA TTTCCAACCA TTTGCCGTGA AGATCCTGAG 
ATACACGGCT ACTTCCGAGA CCCCCGCTGC TTGGGGGAAC AGGAGTATTT CAGTAGCGAG 
GAGTGCTGTG AGGACGACAG CTCTCCCACC TGGAGCAGGC AAAACTACAA CTACTACAAC 
AGGTACCCAG GCAGCTCCAT GGACTTTGAG AGGCCCCGAG GCTACCACCA CCCCCAAGGC 
TTCTTAGAAG ATGATGACTC TCCCACTGGC TATGATTCAC GGAGATCTCC GAGGAGACGT 
CTTCTACCTC CTACCCCACC ATCCCATCGG AGGTCCTCCT TCAACTTTGA GTGTCTGCGC 
AGGCAAAGCA GCCAAGACGA TGTCCTTCCA TCGCCTGCCC TGCCTCACCG CGCTGCCCTG 
CCCCTGCACC TGATGCAGCA GCAGATCATG GCCGTTGCAG GCCTAGATTC CAGTAAAGCC 
CAGAAGTACT CACCGAGCCA CTCTACCCGG TCGTGGGCCA CCCCTCCAGC AACCCCTCCA 
TACCGAGACT GGAGCCCATG CTACACCCCT TTGATCCAGG TGGACCGCTC AGAATCTATG 
GACCAGGTCA ATGGCAGCCT GCCATCCCTA CACCGAAGCT CCTGGTACAC AGATGAACCC 
GACATCTCCT ATAGGACTTT CACGCCAGCT AGTCTGACTG TCCCCAGCAG CTTCCGGAAC 
AAGAACAGCG ACAAGCAGAG GAGTGCAGAC AGCCTGGTGG AGGCAGTCCT GATATCCGAA 
GGCTTGGGAC GCTACGCGAG GGACCCCAAA TTTGTCTCAG CGACAAAACA TGAGATTGCC 
GATGCCTGTG ACCTAACCAT AGACGAGATG GAAAGTGCTG CCAGCACCCT GCTCAATGGC 
AGTGTGTGTC CTCGAGCCAA TGGGGACATG GGCCCCATCT CCCACCGCCA GGACTATGAG 
CTCCAGGACT TTGGTCCTGG CTACAGTGAC GAGGAGCCAG ACCCTGGGCG AGAGGAAGAG 
GACCTGGCAG ATGAGATGAT TTGCATTACC ACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171312.1
CDS	494..7012
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315823.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315823.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAACTTCTCA GCAGGGGGGA AGGCATCCGG ACACTGCTGT GGACCTTCAT CAAGTCCTTT 
CAGGCGCTGC CGTATGTTGC CCTCCTCATT GCCATGCTCT TCTTCATCTA CGCTGTCATA 
GGCATGCAGA TGTTTGGGAA AGTTGCCATG AGAGATAACA ACCAGATCAA TAGGAACAAC 
AACTTCCAGA CCTTTCCTCA GGCAGTGCTC CTACTCTTCA GGTGTGCAAC AGGGGAAGCC 
TGGCAGGAGA TCATGCTTGC CTGCCTCCCA GGAAAGCTGT GCGACCCAGA CTCCGATTAC 
AACCCAGGAG AGGAGTACAC TTGTGGGAGC AACTTTGCCA TTGTCTACTT CATCAGCTTT 
TACATGCTCT GTGCATTCCT GATCATCAAC CTCTTTGTGG CTGTCATCAT GGACAATTTT 
GACTATCTGA CTCGGGACTG GTCTATTCTG GGGCCTCACC ACTTGGACGA ATTCAAAAGA 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AACACCTTGA TGTGGTCACT 
CTGCTCCGGC GGATCCAGCC TCCCCTGGGG TTTGGAAAAT TATGCCCACA CCGAGTAGCA 
TGTAAGAGAT TGGTTGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTCAAT 
GCAACCCTGT TTGCTTTGGT CCGGACAGCT CTCAAGATCA AGACTGAAGG GAACCTGGAG 
CAAGCTAATG AAGAACTCCG AGCTGTGATC AAGAAAATCT GGAAGAAGAC AAGCATGAAG 
CTGCTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA GCAAGGACTG 
GTGGGGAAGT ACCCTGCGAA GAACACCACG ATTGCCCTAC AGGCGGGATT AAGGACCCTG 
CATGACATTG GGCCAGAAAT CCGACGGGCT ATATCCTGTG ACTTGCAAGA TGACGAGCCA 
GAAGACTCCA AACCAGAAGA AGAAGATGTA TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC 
CATGTCAATC ATGTTAATAG TGATAGGAGA GATTCCCTTC AGCAGACCAA TACCACCCAC 
CGTCCCCTGC ATGTCCAAAG GCCTTCAATG CCACCTGCAA GTGATACTGA GAAACCGCTG 
TTTCCTCCAG CAGGAAATTC GGGGTGTCAT AACCATCATA ACCATAATTC CATAGGGAAG 
CAAGCTCCCA CCTCAACAAA TGCCAATCTC AATAATGCCA ATATGTCCAA AGCTGCCCAC 
GGAAAGCCGC CCAGCATTGG GAACCTTGAG CATGTGTCTG AAAATGGGCA TTACTCCTGC 
AAGCATGATC GAGAGCTTCA AAGAAGGTCC AGTATTAAAA GAACCCGCTA TTACGAGACT 
TACATTAGGT CTGAGTCAGG AGATGAGCAA TTTCCAACCA TTTGCCGTGA AGATCCTGAG 
ATACACGGCT ACTTCCGAGA CCCCCGCTGC TTGGGGGAAC AGGAGTATTT CAGTAGCGAG 
GAGTGCTGTG AGGACGACAG CTCTCCCACC TGGAGCAGGC AAAACTACAA CTACTACAAC 
AGGTACCCAG GCAGCTCCAT GGACTTTGAG AGGCCCCGAG GCTACCACCA CCCCCAAGGC 
TTCTTAGAAG ATGATGACTC TCCCACTGGC TATGATTCAC GGAGATCTCC GAGGAGACGT 
CTTCTACCTC CTACCCCACC ATCCCATCGG AGGTCCTCCT TCAACTTTGA GTGTCTGCGC 
AGGCAAAGCA GCCAAGACGA TGTCCTTCCA TCGCCTGCCC TGCCTCACCG CGCTGCCCTG 
CCCCTGCACC TGATGCAGCA GCAGATCATG GCCGTTGCAG GCCTAGATTC CAGTAAAGCC 
CAGAAGTACT CACCGAGCCA CTCTACCCGG TCGTGGGCCA CCCCTCCAGC AACCCCTCCA 
TACCGAGACT GGAGCCCATG CTACACCCCT TTGATCCAGG TGGACCGCTC AGAATCTATG 
GACCAGGTCA ATGGCAGCCT GCCATCCCTA CACCGAAGCT CCTGGTACAC AGATGAACCC 
GACATCTCCT ATAGGACTTT CACGCCAGCT AGTCTGACTG TCCCCAGCAG CTTCCGGAAC 
AAGAACAGCG ACAAGCAGAG GAGTGCAGAC AGCCTGGTGG AGGCAGTCCT GATATCCGAA 
GGCTTGGGAC GCTACGCGAG GGACCCCAAA TTTGTCTCAG CGACAAAACA TGAGATTGCC 
GATGCCTGTG ACCTAACCAT AGACGAGATG GAAAGTGCTG CCAGCACCCT GCTCAATGGC 
AGTGTGTGTC CTCGAGCCAA TGGGGACATG GGCCCCATCT CCCACCGCCA GGACTATGAG 
CTCCAGGACT TTGGTCCTGG CTACAGTGAC GAGGAGCCAG ACCCTGGGCG AGAGGAAGAG 
GACCTGGCAG ATGAGATGAT TTGCATTACC ACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315824.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171313.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X21, mRNA.

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CloneID	OMu78654
Clone ID Related Accession (Same CDS sequence)	XM_017315824.1 , XM_017315824.2
Accession Version	XM_017315824.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171313.1
CDS	561..6995
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315824.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315824.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315824.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171313.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X21, mRNA.

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CloneID	OMu78654
Clone ID Related Accession (Same CDS sequence)	XM_017315824.1 , XM_017315824.2
Accession Version	XM_017315824.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315824.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171313.1
CDS	494..6928
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315824.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315824.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315825.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171314.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X22, mRNA.

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CloneID	OMu78655
Clone ID Related Accession (Same CDS sequence)	XM_017315825.1 , XM_017315825.2
Accession Version	XM_017315825.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171314.1
CDS	561..6995
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315825.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315825.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_017315825.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171314.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X22, mRNA.

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CloneID	OMu78655
Clone ID Related Accession (Same CDS sequence)	XM_017315825.1 , XM_017315825.2
Accession Version	XM_017315825.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315825.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171314.1
CDS	494..6928
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017315825.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315825.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( NM_028981.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083257.2 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant 1, mRNA.

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CloneID	OMu20903
Clone ID Related Accession (Same CDS sequence)	NM_028981.3
Accession Version	NM_028981.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ437292.1, AK147415.1, AJ437291.1, AK032552.1 and BP761485.1. On Nov 5, 2014 this sequence version replaced NM_028981.2. Transcript Variant: This variant (1) differs in the 5' UTR and has multiple coding region differences, compared to variant 3. These differences cause translation initiation at an alternate start codon, compared to variant 3. This results in a distinct N-terminus and shorter protein (isoform 1), compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCTTC CGACATTTTC TAGTGATCTA ATTTTAATTA AAAGCGTTCT CTCCCAAGAG 
ACTGATGCCC GATATAAAGG CAGAGTTGTA AGTGCGGTAG AAAGCACGGA GGACTTCTCC 
CAGGCCTTTG CAGAGGCAAA CTATGCAAGA GGCACCAGAC TCCCTATTTC TGGTGAAGGA 
CCAACTTCTC AGCCCAACAG CTCCAAGCAA ACTGTCCTAT CTTGGCAAGC TGCAATCGAT 
GCTGCTAGAC AGGCCAAGGC CGCCCAGACC ATGAGCACCT CTGCACCCCC ACCTGTAGGA 
TCTCTCTCCC AAAGAAAACG TCAGCAATAC GCCAAGAGCA AAAAACAGGG TAACTCGTCT 
AACAGCCGAC CTGCCCGTGC CCTTTTCTGT TTATCCCTCA ATAACCCCAT CCGAAGAGCC 
TGCATTAGTA TAGTGGAATG GAAACCATTT GACATATTTA TATTATTGGC TATTTTTGCC 
AATTGTGTGG CCTTAGCTAT TTACATCCCA TTCCCTGAAG ATGATTCTAA TTCAACAAAT 
CATAACTTGG AAAAAGTAGA ATATGCCTTC CTGATTATTT TTACAGTGGA GACATTTCTG 
AAGATTATAG CGTATGGACT GTTGCTGCAT CCTAATGCTT ACGTTAGGAA TGGATGGAAT 
TTACTGGATT TTGTTATAGT AATTGTAGGA TTGTTTAGTG TAATTCTGGA ACAATTAACC 
AAAGAAACAG AAGGCGGGAA CCACTCTAGT GGCAAATCAG GAGGCTTTGA TGTCAAAGCC 
CTCCGTGCCT TCCGAGTGTT AAGACCACTT CGACTTGTAT CAGGAGTGCC CAGTTTACAA 
GTTGTTCTGA ACTCCATTAT AAAAGCCATG GTCCCCCTCC TTCACATAGC CCTTTTGGTA 
TTATTTGTAA TTATCATCTA TGCTATTATA GGATTGGAAC TTTTTATTGG AAAAATGCAC 
AAAACATGTT TTTTTGCTGA CTCAGATATA GTAGCTGAAG AGGACCCAGC TCCGTGTGCG 
TTCTCAGGGA ATGGACGCCA GTGCACCGCC AATGGCACGG AATGTAGGAG TGGCTGGGTT 
GGCCCCAATG GAGGCATCAC TAACTTTGAT AACTTTGCCT TTGCCATGCT CACTGTGTTC 
CAGTGCATTA CCATGGAGGG CTGGACAGAT GTGCTCTACT GGGTTAATGA TGCGATAGGA 
TGGGAATGGC CATGGGTGTA TTTTGTTAGT CTGATCATCC TTGGCTCATT TTTCGTCCTT 
AACCTGGTTC TTGGTGTCCT TAGTGGAGAA TTCTCCAAGG AAAGAGAGAA GGCTAAAGCT 
CGTGGAGATT TCCAGAAGCT TCGGGAAAAA CAGCAGCTGG AAGAGGACCT CAAGGGCTAC 
CTGGACTGGA TCACCCAGGC AGAAGACATC GATCCCGAGA ACGAGGAGGA GGGTGGCGAG 
GAAGGCAAAC GAAACACTAG CATGCCCACC AGCGAGACTG AATCTGTCAA CACAGAGAAC 
GTAAGTGGTG AAGGCGAGAC CCAGGGATGC TGTGGAACTC TCTGTCAGGC CATCTCGAAA 
TCCAAACTCA GCCGTCGATG GCGTCGCTGG AACCGGTTCA ATCGCAGGAG GTGCAGGGCT 
GCCGTGAAGT CTGTCACGTT TTACTGGCTG GTGATTGTCC TGGTGTTTCT CAACACTTTA 
ACTATTTCCT CTGAGCATTA CAATCAGCCA GACTGGCTGA CACAGATTCA AGACATCGCA 
AACAAAGTCC TCTTGGCTCT GTTCACCTGT GAGATGCTGG TGAAGATGTA CAGCCTGGGC 
CTCCAGGCTT ACTTTGTCTC CCTCTTCAAC CGCTTCGATT GCTTTGTGGT GTGTGGGGGC 
ATCACCGAAA CCATCCTGGT GGAACTGGAG CTCATGTCTC CCCTGGGGGT CTCCGTGTTC 
CGGTGTGTGC GCCTCCTGAG AATCTTCAAA GTGACCAGGC ACTGGACCTC CCTGAGCAAC 
TTGGTGGCAT CCTTGTTAAA CTCCATGAAG TCCATCGCCT CTCTGCTGTT ACTCCTCTTC 
CTCTTCATCA TCATCTTCTC CTTGCTGGGG ATGCAGTTGT TTGGTGGGAA GTTTAATTTT 
GACGAGACTC AAACCAAGCG AAGCACCTTT GACAACTTTC CGCAAGCACT CCTGACGGTG 
TTCCAGATCC TGACAGGTGA AGACTGGAAT GCGGTGATGT ATGACGGCAT CATGGCCTAT 
GGAGGCCCTT CCTCTTCCGG GATGATTGTC TGTATCTACT TCATCATCCT TTTCATCTGT 
GGTAACTATA TCCTGCTGAA TGTCTTCTTG GCCATCGCTG TGGACAATTT GGCTGATGCT 
GAAAGTCTGA ACACTGCTCA GAAAGAGGAA GCTGAAGAAA AAGAAAGGAA AAAGATTGCC 
AGAAAAGAAA GCCTAGAAAA CAAAAAGAAC AACAAACCAG AAGTCAACCA GATAGCCAAC 
AGTGACAACA AGGTTACAAT TGATGATTAT CAGGAAGATG CTGAAGACAA GGACCCTTAC 
CCGCCCTGTG ATGTGCCAGT AGGTGAAGAG GAAGAGGAGG AGGAAGAGGA TGAGCCCGAA 
GTTCCTGCTG GTCCCCGCCC TCGCAGAATC TCAGAGTTGA ACATGAAGGA GAAGATTGCG 
CCCATCCCAG AAGGAAGCGC CTTCTTCATT CTTAGCAAGA CCAACCCGAT ACGTGTGGGT 
TGCCACAAGC TCATTAACCA CCACATCTTC ACCAACCTTA TCCTGGTCTT CATCATGCTG 
AGCAGCGCTG CCCTGGCTGC AGAGGACCCC ATCCGTAGCC ACTCATTCCG CAACACGATA 
CTGGGCTACT TTGACTATGC CTTCACAGCC ATCTTTACGG TTGAAATCCT GTTAAAGATG 
ACAACTTTTG GAGCCTTCTT GCACAAAGGG GCTTTCTGCA GGAACTACTT CAATTTGCTG 
GACATGCTGG TTGTTGGGGT GTCGCTGGTG TCCTTTGGGA TTCAGTCCAG CGCCATCTCC 
GTTGTGAAGA TTCTGAGGGT TTTGAGGGTC TTGCGGCCTC TCAGAGCAAT CAACAGAGCA 
AAGGGACTTA AGCACGTGGT CCAGTGTGTC TTTGTGGCCA TCCGAACCAT TGGCAACATC 
ATGATCGTCA CGACCCTGCT CCAGTTCATG TTTGCATGCA TTGGGGTCCA GCTGTTCAAG 
GGGAAGTTCT ACCGTTGCAC AGATGAAGCC AAAAGTAACC CTGAGGAGTG CAGGGGGCTT 
TTCATTCTTT ATAAGGACGG CGATGTTGAC AGTCCTGTGG TCCGTGAGAG GATCTGGCAA 
AATAGTGATT TCAATTTTGA CAATGTCCTT TCGGCTATGA TGGCGCTGTT CACAGTCTCA 
ACTTTTGAGG GCTGGCCCGC GTTGCTGTAC AAAGCTATTG ATTCAAACGG TGAGAACGTT 
GGTCCTGTCT ACAACTACCG TGTGGAGATC TCCATCTTCT TCATCATCTA CATCATCATC 
GTTGCCTTCT TCATGATGAA CATCTTTGTG GGCTTCGTCA TCGTCACCTT CCAGGAACAG 
GGAGAAAAAG AGTATAAGAA CTGTGAGCTG GACAAAAATC AGCGTCAGTG TGTTGAATAT 
GCCTTGAAAG CACGCCCCTT AAGGAGATAC ATCCCCAAAA ACCCATACCA GTACAAGTTC 
TGGTATGTGG TGAACTCCTC GCCTTTCGAA TACATGATGT TCGTCCTCAT CATGCTCAAC 
ACGCTCTGCC TGGCCATGCA GCACTATGAG CAGTCCAAGA TGTTCAATGA TGCCATGGAC 
ATTCTGAACA TGGTCTTCAC AGGGGTCTTC ACTGTTGAGA TGGTTTTGAA AGTCATTGCT 
TTCAAGCCCA AGGGGTATTT TAGTGACGCC TGGAACACGT TTGACTCCCT CATCGTAATC 
GGCAGCATTA TAGACGTGGC CCTCAGCGAA GCCGACAACT CTGAAGAGAG CAATAGAATC 
TCCATCACCT TTTTCCGTCT TTTCCGAGTG ATGCGGTTGG TGAAACTTCT CAGCAGGGGG 
GAAGGCATCC GGACACTGCT GTGGACCTTC ATCAAGTCCT TTCAGGCGCT GCCGTATGTT 
GCCCTCCTCA TTGCCATGCT CTTCTTCATC TACGCTGTCA TAGGCATGCA GATGTTTGGG 
AAAGTTGCCA TGAGAGATAA CAACCAGATC AATAGGAACA ACAACTTCCA GACCTTTCCT 
CAGGCAGTGC TCCTACTCTT CAGGTGTGCA ACAGGGGAAG CCTGGCAGGA GATCATGCTT 
GCCTGCCTCC CAGGAAAGCT GTGCGACCCA GACTCCGATT ACAACCCAGG AGAGGAGTAC 
ACTTGTGGGA GCAACTTTGC CATTGTCTAC TTCATCAGCT TTTACATGCT CTGTGCATTC 
CTGATCATCA ACCTCTTTGT GGCTGTCATC ATGGACAATT TTGACTATCT GACTCGGGAC 
TGGTCTATTC TGGGGCCTCA CCACTTGGAC GAATTCAAAA GAATATGGTC AGAATATGAC 
CCTGAAGCAA AGGGAAGGAT AAAACACCTT GATGTGGTCA CTCTGCTCCG GCGGATCCAG 
CCTCCCCTGG GGTTTGGAAA ATTATGCCCA CACCGAGTAG CATGTAAGAG ATTGGTTGCC 
ATGAACATGC CTCTCAACAG TGATGGGACA GTCATGTTCA ATGCAACCCT GTTTGCTTTG 
GTCCGGACAG CTCTCAAGAT CAAGACTGAA GGGAACCTGG AGCAAGCTAA TGAAGAACTC 
CGAGCTGTGA TCAAGAAAAT CTGGAAGAAG ACAAGCATGA AGCTGCTTGA CCAAGTTGTC 
CCTCCAGCTG GTGATGATGA GGTAACCGTG GGGAAGTTCT ATGCCACTTT CCTGATACAG 
GACTACTTTA GGAAATTCAA GAAACGGAAA GAGCAAGGAC TGGTGGGGAA GTACCCTGCG 
AAGAACACCA CGATTGCCCT ACAGGCGGGA TTAAGGACCC TGCATGACAT TGGGCCAGAA 
ATCCGACGGG CTATATCCTG TGACTTGCAA GATGACGAGC CAGAAGACTC CAAACCAGAA 
GAAGAAGATG TATTCAAAAG AAATGGTGCC CTGCTTGGAA ACCATGTCAA TCATGTTAAT 
AGTGATAGGA GAGATTCCCT TCAGCAGACC AATACCACCC ACCGTCCCCT GCATGTCCAA 
AGGCCTTCAA TGCCACCTGC AAGTGATACT GAGAAACCGC TGTTTCCTCC AGCAGGAAAT 
TCGGGGTGTC ATAACCATCA TAACCATAAT TCCATAGGGA AGCAAGCTCC CACCTCAACA 
AATGCCAATC TCAATAATGC CAATATGTCC AAAGCTGCCC ACGGAAAGCC GCCCAGCATT 
GGGAACCTTG AGCATGTGTC TGAAAATGGG CATTACTCCT GCAAGCATGA TCGAGAGCTT 
CAAAGAAGGT CCAGTATTAA AAGAACCCGC TATTACGAGA CTTACATTAG GTCTGAGTCA 
GGAGATGAGC AATTTCCAAC CATTTGCCGT GAAGATCCTG AGATACACGG CTACTTCCGA 
GACCCCCGCT GCTTGGGGGA ACAGGAGTAT TTCAGTAGCG AGGAGTGCTG TGAGGACGAC 
AGCTCTCCCA CCTGGAGCAG GCAAAACTAC AACTACTACA ACAGGTACCC AGGCAGCTCC 
ATGGACTTTG AGAGGCCCCG AGGCTACCAC CACCCCCAAG GCTTCTTAGA AGATGATGAC 
TCTCCCACTG GCTATGATTC ACGGAGATCT CCGAGGAGAC GTCTTCTACC TCCTACCCCA 
CCATCCCATC GGAGGTCCTC CTTCAACTTT GAGTGTCTGC GCAGGCAAAG CAGCCAAGAC 
GATGTCCTTC CATCGCCTGC CCTGCCTCAC CGCGCTGCCC TGCCCCTGCA CCTGATGCAG 
CAGCAGATCA TGGCCGTTGC AGGCCTAGAT TCCAGTAAAG CCCAGAAGTA CTCACCGAGC 
CACTCTACCC GGTCGTGGGC CACCCCTCCA GCAACCCCTC CATACCGAGA CTGGAGCCCA 
TGCTACACCC CTTTGATCCA GGTGGACCGC TCAGAATCTA TGGACCAGGT CAATGGCAGC 
CTGCCATCCC TACACCGAAG CTCCTGGTAC ACAGATGAAC CCGACATCTC CTATAGGACT 
TTCACGCCAG CTAGTCTGAC TGTCCCCAGC AGCTTCCGGA ACAAGAACAG CGACAAGCAG 
AGGAGTGCAG ACAGCCTGGT GGAGGCAGTC CTGATATCCG AAGGCTTGGG ACGCTACGCG 
AGGGACCCCA AATTTGTCTC AGCGACAAAA CATGAGATTG CCGATGCCTG TGACCTAACC 
ATAGACGAGA TGGAAAGTGC TGCCAGCACC CTGCTCAATG GCAGTGTGTG TCCTCGAGCC 
AATGGGGACA TGGGCCCCAT CTCCCACCGC CAGGACTATG AGCTCCAGGA CTTTGGTCCT 
GGCTACAGTG ACGAGGAGCC AGACCCTGGG CGAGAGGAAG AGGACCTGGC AGATGAGATG 
ATTTGCATTA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083257.2
CDS	56..6556
Translation	MNLPTFSSDLILIKSVLSQETDARYKGRVVSAVESTEDFSQAFAEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	NM_028981.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028981.3

ORF Insert Sequence:

ATGAACCTTC CGACATTTTC TAGTGATCTA ATTTTAATTA AAAGCGTTCT CTCCCAAGAG 
ACTGATGCCC GATATAAAGG CAGAGTTGTA AGTGCGGTAG AAAGCACGGA GGACTTCTCC 
CAGGCCTTTG CAGAGGCAAA CTATGCAAGA GGCACCAGAC TCCCTATTTC TGGTGAAGGA 
CCAACTTCTC AGCCCAACAG CTCCAAGCAA ACTGTCCTAT CTTGGCAAGC TGCAATCGAT 
GCTGCTAGAC AGGCCAAGGC CGCCCAGACC ATGAGCACCT CTGCACCCCC ACCTGTAGGA 
TCTCTCTCCC AAAGAAAACG TCAGCAATAC GCCAAGAGCA AAAAACAGGG TAACTCGTCT 
AACAGCCGAC CTGCCCGTGC CCTTTTCTGT TTATCCCTCA ATAACCCCAT CCGAAGAGCC 
TGCATTAGTA TAGTGGAATG GAAACCATTT GACATATTTA TATTATTGGC TATTTTTGCC 
AATTGTGTGG CCTTAGCTAT TTACATCCCA TTCCCTGAAG ATGATTCTAA TTCAACAAAT 
CATAACTTGG AAAAAGTAGA ATATGCCTTC CTGATTATTT TTACAGTGGA GACATTTCTG 
AAGATTATAG CGTATGGACT GTTGCTGCAT CCTAATGCTT ACGTTAGGAA TGGATGGAAT 
TTACTGGATT TTGTTATAGT AATTGTAGGA TTGTTTAGTG TAATTCTGGA ACAATTAACC 
AAAGAAACAG AAGGCGGGAA CCACTCTAGT GGCAAATCAG GAGGCTTTGA TGTCAAAGCC 
CTCCGTGCCT TCCGAGTGTT AAGACCACTT CGACTTGTAT CAGGAGTGCC CAGTTTACAA 
GTTGTTCTGA ACTCCATTAT AAAAGCCATG GTCCCCCTCC TTCACATAGC CCTTTTGGTA 
TTATTTGTAA TTATCATCTA TGCTATTATA GGATTGGAAC TTTTTATTGG AAAAATGCAC 
AAAACATGTT TTTTTGCTGA CTCAGATATA GTAGCTGAAG AGGACCCAGC TCCGTGTGCG 
TTCTCAGGGA ATGGACGCCA GTGCACCGCC AATGGCACGG AATGTAGGAG TGGCTGGGTT 
GGCCCCAATG GAGGCATCAC TAACTTTGAT AACTTTGCCT TTGCCATGCT CACTGTGTTC 
CAGTGCATTA CCATGGAGGG CTGGACAGAT GTGCTCTACT GGGTTAATGA TGCGATAGGA 
TGGGAATGGC CATGGGTGTA TTTTGTTAGT CTGATCATCC TTGGCTCATT TTTCGTCCTT 
AACCTGGTTC TTGGTGTCCT TAGTGGAGAA TTCTCCAAGG AAAGAGAGAA GGCTAAAGCT 
CGTGGAGATT TCCAGAAGCT TCGGGAAAAA CAGCAGCTGG AAGAGGACCT CAAGGGCTAC 
CTGGACTGGA TCACCCAGGC AGAAGACATC GATCCCGAGA ACGAGGAGGA GGGTGGCGAG 
GAAGGCAAAC GAAACACTAG CATGCCCACC AGCGAGACTG AATCTGTCAA CACAGAGAAC 
GTAAGTGGTG AAGGCGAGAC CCAGGGATGC TGTGGAACTC TCTGTCAGGC CATCTCGAAA 
TCCAAACTCA GCCGTCGATG GCGTCGCTGG AACCGGTTCA ATCGCAGGAG GTGCAGGGCT 
GCCGTGAAGT CTGTCACGTT TTACTGGCTG GTGATTGTCC TGGTGTTTCT CAACACTTTA 
ACTATTTCCT CTGAGCATTA CAATCAGCCA GACTGGCTGA CACAGATTCA AGACATCGCA 
AACAAAGTCC TCTTGGCTCT GTTCACCTGT GAGATGCTGG TGAAGATGTA CAGCCTGGGC 
CTCCAGGCTT ACTTTGTCTC CCTCTTCAAC CGCTTCGATT GCTTTGTGGT GTGTGGGGGC 
ATCACCGAAA CCATCCTGGT GGAACTGGAG CTCATGTCTC CCCTGGGGGT CTCCGTGTTC 
CGGTGTGTGC GCCTCCTGAG AATCTTCAAA GTGACCAGGC ACTGGACCTC CCTGAGCAAC 
TTGGTGGCAT CCTTGTTAAA CTCCATGAAG TCCATCGCCT CTCTGCTGTT ACTCCTCTTC 
CTCTTCATCA TCATCTTCTC CTTGCTGGGG ATGCAGTTGT TTGGTGGGAA GTTTAATTTT 
GACGAGACTC AAACCAAGCG AAGCACCTTT GACAACTTTC CGCAAGCACT CCTGACGGTG 
TTCCAGATCC TGACAGGTGA AGACTGGAAT GCGGTGATGT ATGACGGCAT CATGGCCTAT 
GGAGGCCCTT CCTCTTCCGG GATGATTGTC TGTATCTACT TCATCATCCT TTTCATCTGT 
GGTAACTATA TCCTGCTGAA TGTCTTCTTG GCCATCGCTG TGGACAATTT GGCTGATGCT 
GAAAGTCTGA ACACTGCTCA GAAAGAGGAA GCTGAAGAAA AAGAAAGGAA AAAGATTGCC 
AGAAAAGAAA GCCTAGAAAA CAAAAAGAAC AACAAACCAG AAGTCAACCA GATAGCCAAC 
AGTGACAACA AGGTTACAAT TGATGATTAT CAGGAAGATG CTGAAGACAA GGACCCTTAC 
CCGCCCTGTG ATGTGCCAGT AGGTGAAGAG GAAGAGGAGG AGGAAGAGGA TGAGCCCGAA 
GTTCCTGCTG GTCCCCGCCC TCGCAGAATC TCAGAGTTGA ACATGAAGGA GAAGATTGCG 
CCCATCCCAG AAGGAAGCGC CTTCTTCATT CTTAGCAAGA CCAACCCGAT ACGTGTGGGT 
TGCCACAAGC TCATTAACCA CCACATCTTC ACCAACCTTA TCCTGGTCTT CATCATGCTG 
AGCAGCGCTG CCCTGGCTGC AGAGGACCCC ATCCGTAGCC ACTCATTCCG CAACACGATA 
CTGGGCTACT TTGACTATGC CTTCACAGCC ATCTTTACGG TTGAAATCCT GTTAAAGATG 
ACAACTTTTG GAGCCTTCTT GCACAAAGGG GCTTTCTGCA GGAACTACTT CAATTTGCTG 
GACATGCTGG TTGTTGGGGT GTCGCTGGTG TCCTTTGGGA TTCAGTCCAG CGCCATCTCC 
GTTGTGAAGA TTCTGAGGGT TTTGAGGGTC TTGCGGCCTC TCAGAGCAAT CAACAGAGCA 
AAGGGACTTA AGCACGTGGT CCAGTGTGTC TTTGTGGCCA TCCGAACCAT TGGCAACATC 
ATGATCGTCA CGACCCTGCT CCAGTTCATG TTTGCATGCA TTGGGGTCCA GCTGTTCAAG 
GGGAAGTTCT ACCGTTGCAC AGATGAAGCC AAAAGTAACC CTGAGGAGTG CAGGGGGCTT 
TTCATTCTTT ATAAGGACGG CGATGTTGAC AGTCCTGTGG TCCGTGAGAG GATCTGGCAA 
AATAGTGATT TCAATTTTGA CAATGTCCTT TCGGCTATGA TGGCGCTGTT CACAGTCTCA 
ACTTTTGAGG GCTGGCCCGC GTTGCTGTAC AAAGCTATTG ATTCAAACGG TGAGAACGTT 
GGTCCTGTCT ACAACTACCG TGTGGAGATC TCCATCTTCT TCATCATCTA CATCATCATC 
GTTGCCTTCT TCATGATGAA CATCTTTGTG GGCTTCGTCA TCGTCACCTT CCAGGAACAG 
GGAGAAAAAG AGTATAAGAA CTGTGAGCTG GACAAAAATC AGCGTCAGTG TGTTGAATAT 
GCCTTGAAAG CACGCCCCTT AAGGAGATAC ATCCCCAAAA ACCCATACCA GTACAAGTTC 
TGGTATGTGG TGAACTCCTC GCCTTTCGAA TACATGATGT TCGTCCTCAT CATGCTCAAC 
ACGCTCTGCC TGGCCATGCA GCACTATGAG CAGTCCAAGA TGTTCAATGA TGCCATGGAC 
ATTCTGAACA TGGTCTTCAC AGGGGTCTTC ACTGTTGAGA TGGTTTTGAA AGTCATTGCT 
TTCAAGCCCA AGGGGTATTT TAGTGACGCC TGGAACACGT TTGACTCCCT CATCGTAATC 
GGCAGCATTA TAGACGTGGC CCTCAGCGAA GCCGACAACT CTGAAGAGAG CAATAGAATC 
TCCATCACCT TTTTCCGTCT TTTCCGAGTG ATGCGGTTGG TGAAACTTCT CAGCAGGGGG 
GAAGGCATCC GGACACTGCT GTGGACCTTC ATCAAGTCCT TTCAGGCGCT GCCGTATGTT 
GCCCTCCTCA TTGCCATGCT CTTCTTCATC TACGCTGTCA TAGGCATGCA GATGTTTGGG 
AAAGTTGCCA TGAGAGATAA CAACCAGATC AATAGGAACA ACAACTTCCA GACCTTTCCT 
CAGGCAGTGC TCCTACTCTT CAGGTGTGCA ACAGGGGAAG CCTGGCAGGA GATCATGCTT 
GCCTGCCTCC CAGGAAAGCT GTGCGACCCA GACTCCGATT ACAACCCAGG AGAGGAGTAC 
ACTTGTGGGA GCAACTTTGC CATTGTCTAC TTCATCAGCT TTTACATGCT CTGTGCATTC 
CTGATCATCA ACCTCTTTGT GGCTGTCATC ATGGACAATT TTGACTATCT GACTCGGGAC 
TGGTCTATTC TGGGGCCTCA CCACTTGGAC GAATTCAAAA GAATATGGTC AGAATATGAC 
CCTGAAGCAA AGGGAAGGAT AAAACACCTT GATGTGGTCA CTCTGCTCCG GCGGATCCAG 
CCTCCCCTGG GGTTTGGAAA ATTATGCCCA CACCGAGTAG CATGTAAGAG ATTGGTTGCC 
ATGAACATGC CTCTCAACAG TGATGGGACA GTCATGTTCA ATGCAACCCT GTTTGCTTTG 
GTCCGGACAG CTCTCAAGAT CAAGACTGAA GGGAACCTGG AGCAAGCTAA TGAAGAACTC 
CGAGCTGTGA TCAAGAAAAT CTGGAAGAAG ACAAGCATGA AGCTGCTTGA CCAAGTTGTC 
CCTCCAGCTG GTGATGATGA GGTAACCGTG GGGAAGTTCT ATGCCACTTT CCTGATACAG 
GACTACTTTA GGAAATTCAA GAAACGGAAA GAGCAAGGAC TGGTGGGGAA GTACCCTGCG 
AAGAACACCA CGATTGCCCT ACAGGCGGGA TTAAGGACCC TGCATGACAT TGGGCCAGAA 
ATCCGACGGG CTATATCCTG TGACTTGCAA GATGACGAGC CAGAAGACTC CAAACCAGAA 
GAAGAAGATG TATTCAAAAG AAATGGTGCC CTGCTTGGAA ACCATGTCAA TCATGTTAAT 
AGTGATAGGA GAGATTCCCT TCAGCAGACC AATACCACCC ACCGTCCCCT GCATGTCCAA 
AGGCCTTCAA TGCCACCTGC AAGTGATACT GAGAAACCGC TGTTTCCTCC AGCAGGAAAT 
TCGGGGTGTC ATAACCATCA TAACCATAAT TCCATAGGGA AGCAAGCTCC CACCTCAACA 
AATGCCAATC TCAATAATGC CAATATGTCC AAAGCTGCCC ACGGAAAGCC GCCCAGCATT 
GGGAACCTTG AGCATGTGTC TGAAAATGGG CATTACTCCT GCAAGCATGA TCGAGAGCTT 
CAAAGAAGGT CCAGTATTAA AAGAACCCGC TATTACGAGA CTTACATTAG GTCTGAGTCA 
GGAGATGAGC AATTTCCAAC CATTTGCCGT GAAGATCCTG AGATACACGG CTACTTCCGA 
GACCCCCGCT GCTTGGGGGA ACAGGAGTAT TTCAGTAGCG AGGAGTGCTG TGAGGACGAC 
AGCTCTCCCA CCTGGAGCAG GCAAAACTAC AACTACTACA ACAGGTACCC AGGCAGCTCC 
ATGGACTTTG AGAGGCCCCG AGGCTACCAC CACCCCCAAG GCTTCTTAGA AGATGATGAC 
TCTCCCACTG GCTATGATTC ACGGAGATCT CCGAGGAGAC GTCTTCTACC TCCTACCCCA 
CCATCCCATC GGAGGTCCTC CTTCAACTTT GAGTGTCTGC GCAGGCAAAG CAGCCAAGAC 
GATGTCCTTC CATCGCCTGC CCTGCCTCAC CGCGCTGCCC TGCCCCTGCA CCTGATGCAG 
CAGCAGATCA TGGCCGTTGC AGGCCTAGAT TCCAGTAAAG CCCAGAAGTA CTCACCGAGC 
CACTCTACCC GGTCGTGGGC CACCCCTCCA GCAACCCCTC CATACCGAGA CTGGAGCCCA 
TGCTACACCC CTTTGATCCA GGTGGACCGC TCAGAATCTA TGGACCAGGT CAATGGCAGC 
CTGCCATCCC TACACCGAAG CTCCTGGTAC ACAGATGAAC CCGACATCTC CTATAGGACT 
TTCACGCCAG CTAGTCTGAC TGTCCCCAGC AGCTTCCGGA ACAAGAACAG CGACAAGCAG 
AGGAGTGCAG ACAGCCTGGT GGAGGCAGTC CTGATATCCG AAGGCTTGGG ACGCTACGCG 
AGGGACCCCA AATTTGTCTC AGCGACAAAA CATGAGATTG CCGATGCCTG TGACCTAACC 
ATAGACGAGA TGGAAAGTGC TGCCAGCACC CTGCTCAATG GCAGTGTGTG TCCTCGAGCC 
AATGGGGACA TGGGCCCCAT CTCCCACCGC CAGGACTATG AGCTCCAGGA CTTTGGTCCT 
GGCTACAGTG ACGAGGAGCC AGACCCTGGG CGAGAGGAAG AGGACCTGGC AGATGAGATG 
ATTTGCATTA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( NM_001083616.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001077085.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant 2, mRNA.

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CloneID	OMu20905
Clone ID Related Accession (Same CDS sequence)	NM_001083616.2
Accession Version	NM_001083616.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK147415.1, AJ437291.1, AK032552.1 and BP761485.1. On Nov 5, 2014 this sequence version replaced NM_001083616.1. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the coding region, compared to variant 3. This results in a shorter protein (isoform 2), compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077085.1
CDS	496..6930
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	NM_001083616.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083616.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGAAC CCGCTATTAC GAGACTTACA TTAGGTCTGA GTCAGGAGAT 
GAGCAATTTC CAACCATTTG CCGTGAAGAT CCTGAGATAC ACGGCTACTT CCGAGACCCC 
CGCTGCTTGG GGGAACAGGA GTATTTCAGT AGCGAGGAGT GCTGTGAGGA CGACAGCTCT 
CCCACCTGGA GCAGGCAAAA CTACAACTAC TACAACAGGT ACCCAGGCAG CTCCATGGAC 
TTTGAGAGGC CCCGAGGCTA CCACCACCCC CAAGGCTTCT TAGAAGATGA TGACTCTCCC 
ACTGGCTATG ATTCACGGAG ATCTCCGAGG AGACGTCTTC TACCTCCTAC CCCACCATCC 
CATCGGAGGT CCTCCTTCAA CTTTGAGTGT CTGCGCAGGC AAAGCAGCCA AGACGATGTC 
CTTCCATCGC CTGCCCTGCC TCACCGCGCT GCCCTGCCCC TGCACCTGAT GCAGCAGCAG 
ATCATGGCCG TTGCAGGCCT AGATTCCAGT AAAGCCCAGA AGTACTCACC GAGCCACTCT 
ACCCGGTCGT GGGCCACCCC TCCAGCAACC CCTCCATACC GAGACTGGAG CCCATGCTAC 
ACCCCTTTGA TCCAGGTGGA CCGCTCAGAA TCTATGGACC AGGTCAATGG CAGCCTGCCA 
TCCCTACACC GAAGCTCCTG GTACACAGAT GAACCCGACA TCTCCTATAG GACTTTCACG 
CCAGCTAGTC TGACTGTCCC CAGCAGCTTC CGGAACAAGA ACAGCGACAA GCAGAGGAGT 
GCAGACAGCC TGGTGGAGGC AGTCCTGATA TCCGAAGGCT TGGGACGCTA CGCGAGGGAC 
CCCAAATTTG TCTCAGCGAC AAAACATGAG ATTGCCGATG CCTGTGACCT AACCATAGAC 
GAGATGGAAA GTGCTGCCAG CACCCTGCTC AATGGCAGTG TGTGTCCTCG AGCCAATGGG 
GACATGGGCC CCATCTCCCA CCGCCAGGAC TATGAGCTCC AGGACTTTGG TCCTGGCTAC 
AGTGACGAGG AGCCAGACCC TGGGCGAGAG GAAGAGGACC TGGCAGATGA GATGATTTGC 
ATTACCACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( NM_001302637.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001289566.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant 3, mRNA.

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CloneID	OMu67259
Clone ID Related Accession (Same CDS sequence)	NM_001302637.1
Accession Version	NM_001302637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK147415.1, AJ437291.1, AK032552.1 and BP761485.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289566.1
CDS	496..7035
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	NM_001302637.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302637.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_006518475.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518538.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X1, mRNA.

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CloneID	OMu49612
Clone ID Related Accession (Same CDS sequence)	XM_006518475.2 , XM_006518475.1
Accession Version	XM_006518475.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518538.1
CDS	561..7184
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLWCWWKRRGAAKTGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_006518475.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518475.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTTA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
GCGGCCAAGA CCGGGCCCTC TGGGTGTCGG CGGTGGGGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCATCT 
GAAAGTGAGA CTATCCCTCT CCCAACTGCC ACACCTGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTTCAGGC GCTGCCGTAT 
GTTGCCCTCC TCATTGCCAT GCTCTTCTTC ATCTACGCTG TCATAGGCAT GCAGATGTTT 
GGGAAAGTTG CCATGAGAGA TAACAACCAG ATCAATAGGA ACAACAACTT CCAGACCTTT 
CCTCAGGCAG TGCTCCTACT CTTCAGGTGT GCAACAGGGG AAGCCTGGCA GGAGATCATG 
CTTGCCTGCC TCCCAGGAAA GCTGTGCGAC CCAGACTCCG ATTACAACCC AGGAGAGGAG 
TACACTTGTG GGAGCAACTT TGCCATTGTC TACTTCATCA GCTTTTACAT GCTCTGTGCA 
TTCCTGATCA TCAACCTCTT TGTGGCTGTC ATCATGGACA ATTTTGACTA TCTGACTCGG 
GACTGGTCTA TTCTGGGGCC TCACCACTTG GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAACAC CTTGATGTGG TCACTCTGCT CCGGCGGATC 
CAGCCTCCCC TGGGGTTTGG AAAATTATGC CCACACCGAG TAGCATGTAA GAGATTGGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TCAATGCAAC CCTGTTTGCT 
TTGGTCCGGA CAGCTCTCAA GATCAAGACT GAAGGGAACC TGGAGCAAGC TAATGAAGAA 
CTCCGAGCTG TGATCAAGAA AATCTGGAAG AAGACAAGCA TGAAGCTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAGCAAG GACTGGTGGG GAAGTACCCT 
GCGAAGAACA CCACGATTGC CCTACAGGCG GGATTAAGGA CCCTGCATGA CATTGGGCCA 
GAAATCCGAC GGGCTATATC CTGTGACTTG CAAGATGACG AGCCAGAAGA CTCCAAACCA 
GAAGAAGAAG ATGTATTCAA AAGAAATGGT GCCCTGCTTG GAAACCATGT CAATCATGTT 
AATAGTGATA GGAGAGATTC CCTTCAGCAG ACCAATACCA CCCACCGTCC CCTGCATGTC 
CAAAGGCCTT CAATGCCACC TGCAAGTGAT ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA 
AATTCGGGGT GTCATAACCA TCATAACCAT AATTCCATAG GGAAGCAAGC TCCCACCTCA 
ACAAATGCCA ATCTCAATAA TGCCAATATG TCCAAAGCTG CCCACGGAAA GCCGCCCAGC 
ATTGGGAACC TTGAGCATGT GTCTGAAAAT GGGCATTACT CCTGCAAGCA TGATCGAGAG 
CTTCAAAGAA GGTCCAGTAT TAAAAGAACC CGCTATTACG AGACTTACAT TAGGTCTGAG 
TCAGGAGATG AGCAATTTCC AACCATTTGC CGTGAAGATC CTGAGATACA CGGCTACTTC 
CGAGACCCCC GCTGCTTGGG GGAACAGGAG TATTTCAGTA GCGAGGAGTG CTGTGAGGAC 
GACAGCTCTC CCACCTGGAG CAGGCAAAAC TACAACTACT ACAACAGGTA CCCAGGCAGC 
TCCATGGACT TTGAGAGGCC CCGAGGCTAC CACCACCCCC AAGGCTTCTT AGAAGATGAT 
GACTCTCCCA CTGGCTATGA TTCACGGAGA TCTCCGAGGA GACGTCTTCT ACCTCCTACC 
CCACCATCCC ATCGGAGGTC CTCCTTCAAC TTTGAGTGTC TGCGCAGGCA AAGCAGCCAA 
GACGATGTCC TTCCATCGCC TGCCCTGCCT CACCGCGCTG CCCTGCCCCT GCACCTGATG 
CAGCAGCAGA TCATGGCCGT TGCAGGCCTA GATTCCAGTA AAGCCCAGAA GTACTCACCG 
AGCCACTCTA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCATACCG AGACTGGAGC 
CCATGCTACA CCCCTTTGAT CCAGGTGGAC CGCTCAGAAT CTATGGACCA GGTCAATGGC 
AGCCTGCCAT CCCTACACCG AAGCTCCTGG TACACAGATG AACCCGACAT CTCCTATAGG 
ACTTTCACGC CAGCTAGTCT GACTGTCCCC AGCAGCTTCC GGAACAAGAA CAGCGACAAG 
CAGAGGAGTG CAGACAGCCT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAC 
GCGAGGGACC CCAAATTTGT CTCAGCGACA AAACATGAGA TTGCCGATGC CTGTGACCTA 
ACCATAGACG AGATGGAAAG TGCTGCCAGC ACCCTGCTCA ATGGCAGTGT GTGTCCTCGA 
GCCAATGGGG ACATGGGCCC CATCTCCCAC CGCCAGGACT ATGAGCTCCA GGACTTTGGT 
CCTGGCTACA GTGACGAGGA GCCAGACCCT GGGCGAGAGG AAGAGGACCT GGCAGATGAG 
ATGATTTGCA TTACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_006518479.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518542.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X11, mRNA.

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CloneID	OMu49616
Clone ID Related Accession (Same CDS sequence)	XM_006518479.1 , XM_006518479.2
Accession Version	XM_006518479.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518542.1
CDS	561..7040
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_006518479.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518479.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CCATCTGAAA GTGAGACTAT CCCTCTCCCA 
ACTGCCACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACACTGCTG 
TGGACCTTCA TCAAGTCCTT TCAGGCGCTG CCGTATGTTG CCCTCCTCAT TGCCATGCTC 
TTCTTCATCT ACGCTGTCAT AGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA CAACTTCCAG ACCTTTCCTC AGGCAGTGCT CCTACTCTTC 
AGGTGTGCAA CAGGGGAAGC CTGGCAGGAG ATCATGCTTG CCTGCCTCCC AGGAAAGCTG 
TGCGACCCAG ACTCCGATTA CAACCCAGGA GAGGAGTACA CTTGTGGGAG CAACTTTGCC 
ATTGTCTACT TCATCAGCTT TTACATGCTC TGTGCATTCC TGATCATCAA CCTCTTTGTG 
GCTGTCATCA TGGACAATTT TGACTATCTG ACTCGGGACT GGTCTATTCT GGGGCCTCAC 
CACTTGGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTCCGG CGGATCCAGC CTCCCCTGGG GTTTGGAAAA 
TTATGCCCAC ACCGAGTAGC ATGTAAGAGA TTGGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTCAA TGCAACCCTG TTTGCTTTGG TCCGGACAGC TCTCAAGATC 
AAGACTGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTCC GAGCTGTGAT CAAGAAAATC 
TGGAAGAAGA CAAGCATGAA GCTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AGCAAGGACT GGTGGGGAAG TACCCTGCGA AGAACACCAC GATTGCCCTA 
CAGGCGGGAT TAAGGACCCT GCATGACATT GGGCCAGAAA TCCGACGGGC TATATCCTGT 
GACTTGCAAG ATGACGAGCC AGAAGACTCC AAACCAGAAG AAGAAGATGT ATTCAAAAGA 
AATGGTGCCC TGCTTGGAAA CCATGTCAAT CATGTTAATA GTGATAGGAG AGATTCCCTT 
CAGCAGACCA ATACCACCCA CCGTCCCCTG CATGTCCAAA GGCCTTCAAT GCCACCTGCA 
AGTGATACTG AGAAACCGCT GTTTCCTCCA GCAGGAAATT CGGGGTGTCA TAACCATCAT 
AACCATAATT CCATAGGGAA GCAAGCTCCC ACCTCAACAA ATGCCAATCT CAATAATGCC 
AATATGTCCA AAGCTGCCCA CGGAAAGCCG CCCAGCATTG GGAACCTTGA GCATGTGTCT 
GAAAATGGGC ATTACTCCTG CAAGCATGAT CGAGAGCTTC AAAGAAGGTC CAGTATTAAA 
AGAACCCGCT ATTACGAGAC TTACATTAGG TCTGAGTCAG GAGATGAGCA ATTTCCAACC 
ATTTGCCGTG AAGATCCTGA GATACACGGC TACTTCCGAG ACCCCCGCTG CTTGGGGGAA 
CAGGAGTATT TCAGTAGCGA GGAGTGCTGT GAGGACGACA GCTCTCCCAC CTGGAGCAGG 
CAAAACTACA ACTACTACAA CAGGTACCCA GGCAGCTCCA TGGACTTTGA GAGGCCCCGA 
GGCTACCACC ACCCCCAAGG CTTCTTAGAA GATGATGACT CTCCCACTGG CTATGATTCA 
CGGAGATCTC CGAGGAGACG TCTTCTACCT CCTACCCCAC CATCCCATCG GAGGTCCTCC 
TTCAACTTTG AGTGTCTGCG CAGGCAAAGC AGCCAAGACG ATGTCCTTCC ATCGCCTGCC 
CTGCCTCACC GCGCTGCCCT GCCCCTGCAC CTGATGCAGC AGCAGATCAT GGCCGTTGCA 
GGCCTAGATT CCAGTAAAGC CCAGAAGTAC TCACCGAGCC ACTCTACCCG GTCGTGGGCC 
ACCCCTCCAG CAACCCCTCC ATACCGAGAC TGGAGCCCAT GCTACACCCC TTTGATCCAG 
GTGGACCGCT CAGAATCTAT GGACCAGGTC AATGGCAGCC TGCCATCCCT ACACCGAAGC 
TCCTGGTACA CAGATGAACC CGACATCTCC TATAGGACTT TCACGCCAGC TAGTCTGACT 
GTCCCCAGCA GCTTCCGGAA CAAGAACAGC GACAAGCAGA GGAGTGCAGA CAGCCTGGTG 
GAGGCAGTCC TGATATCCGA AGGCTTGGGA CGCTACGCGA GGGACCCCAA ATTTGTCTCA 
GCGACAAAAC ATGAGATTGC CGATGCCTGT GACCTAACCA TAGACGAGAT GGAAAGTGCT 
GCCAGCACCC TGCTCAATGG CAGTGTGTGT CCTCGAGCCA ATGGGGACAT GGGCCCCATC 
TCCCACCGCC AGGACTATGA GCTCCAGGAC TTTGGTCCTG GCTACAGTGA CGAGGAGCCA 
GACCCTGGGC GAGAGGAAGA GGACCTGGCA GATGAGATGA TTTGCATTAC CACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1d ( XM_006518480.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518543.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X15, mRNA.

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CloneID	OMu49617
Clone ID Related Accession (Same CDS sequence)	XM_006518480.1 , XM_006518480.2
Accession Version	XM_006518480.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGGTC TGAGTCAGGA GATGAGCAAT TTCCAACCAT TTGCCGTGAA 
GATCCTGAGA TACACGGCTA CTTCCGAGAC CCCCGCTGCT TGGGGGAACA GGAGTATTTC 
AGTAGCGAGG AGTGCTGTGA GGACGACAGC TCTCCCACCT GGAGCAGGCA AAACTACAAC 
TACTACAACA GGTACCCAGG CAGCTCCATG GACTTTGAGA GGCCCCGAGG CTACCACCAC 
CCCCAAGGCT TCTTAGAAGA TGATGACTCT CCCACTGGCT ATGATTCACG GAGATCTCCG 
AGGAGACGTC TTCTACCTCC TACCCCACCA TCCCATCGGA GGTCCTCCTT CAACTTTGAG 
TGTCTGCGCA GGCAAAGCAG CCAAGACGAT GTCCTTCCAT CGCCTGCCCT GCCTCACCGC 
GCTGCCCTGC CCCTGCACCT GATGCAGCAG CAGATCATGG CCGTTGCAGG CCTAGATTCC 
AGTAAAGCCC AGAAGTACTC ACCGAGCCAC TCTACCCGGT CGTGGGCCAC CCCTCCAGCA 
ACCCCTCCAT ACCGAGACTG GAGCCCATGC TACACCCCTT TGATCCAGGT GGACCGCTCA 
GAATCTATGG ACCAGGTCAA TGGCAGCCTG CCATCCCTAC ACCGAAGCTC CTGGTACACA 
GATGAACCCG ACATCTCCTA TAGGACTTTC ACGCCAGCTA GTCTGACTGT CCCCAGCAGC 
TTCCGGAACA AGAACAGCGA CAAGCAGAGG AGTGCAGACA GCCTGGTGGA GGCAGTCCTG 
ATATCCGAAG GCTTGGGACG CTACGCGAGG GACCCCAAAT TTGTCTCAGC GACAAAACAT 
GAGATTGCCG ATGCCTGTGA CCTAACCATA GACGAGATGG AAAGTGCTGC CAGCACCCTG 
CTCAATGGCA GTGTGTGTCC TCGAGCCAAT GGGGACATGG GCCCCATCTC CCACCGCCAG 
GACTATGAGC TCCAGGACTT TGGTCCTGGC TACAGTGACG AGGAGCCAGA CCCTGGGCGA 
GAGGAAGAGG ACCTGGCAGA TGAGATGATT TGCATTACCA CCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518543.1
CDS	561..6968
Translation	MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRSESGDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_006518480.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518480.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAACATCAAC GGCAGCACCA AGAGGACCAT 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTA TTTCTGGTGA AGGACCAACT 
TCTCAGCCCA ACAGCTCCAA GCAAACTGTC CTATCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCCGCCCA GACCATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCTAACAGC 
CGACCTGCCC GTGCCCTTTT CTGTTTATCC CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TGGAGACATT TCTGAAGATT 
ATAGCGTATG GACTGTTGCT GCATCCTAAT GCTTACGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATTGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC TAGTGGCAAA TCAGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTCCGAG TGTTAAGACC ACTTCGACTT GTATCAGGAG TGCCCAGTTT ACAAGTTGTT 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATTATCA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATAGTAGCT GAAGAGGACC CAGCTCCGTG TGCGTTCTCA 
GGGAATGGAC GCCAGTGCAC CGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCC 
AATGGAGGCA TCACTAACTT TGATAACTTT GCCTTTGCCA TGCTCACTGT GTTCCAGTGC 
ATTACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCC AAGGAAAGAG AGAAGGCTAA AGCTCGTGGA 
GATTTCCAGA AGCTTCGGGA AAAACAGCAG CTGGAAGAGG ACCTCAAGGG CTACCTGGAC 
TGGATCACCC AGGCAGAAGA CATCGATCCC GAGAACGAGG AGGAGGGTGG CGAGGAAGGC 
AAACGAAACA CTAGCATGCC CACCAGCGAG ACTGAATCTG TCAACACAGA GAACGTAAGT 
GGTGAAGGCG AGACCCAGGG ATGCTGTGGA ACTCTCTGTC AGGCCATCTC GAAATCCAAA 
CTCAGCCGTC GATGGCGTCG CTGGAACCGG TTCAATCGCA GGAGGTGCAG GGCTGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTGATT GTCCTGGTGT TTCTCAACAC TTTAACTATT 
TCCTCTGAGC ATTACAATCA GCCAGACTGG CTGACACAGA TTCAAGACAT CGCAAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGTGAGATG CTGGTGAAGA TGTACAGCCT GGGCCTCCAG 
GCTTACTTTG TCTCCCTCTT CAACCGCTTC GATTGCTTTG TGGTGTGTGG GGGCATCACC 
GAAACCATCC TGGTGGAACT GGAGCTCATG TCTCCCCTGG GGGTCTCCGT GTTCCGGTGT 
GTGCGCCTCC TGAGAATCTT CAAAGTGACC AGGCACTGGA CCTCCCTGAG CAACTTGGTG 
GCATCCTTGT TAAACTCCAT GAAGTCCATC GCCTCTCTGC TGTTACTCCT CTTCCTCTTC 
ATCATCATCT TCTCCTTGCT GGGGATGCAG TTGTTTGGTG GGAAGTTTAA TTTTGACGAG 
ACTCAAACCA AGCGAAGCAC CTTTGACAAC TTTCCGCAAG CACTCCTGAC GGTGTTCCAG 
ATCCTGACAG GTGAAGACTG GAATGCGGTG ATGTATGACG GCATCATGGC CTATGGAGGC 
CCTTCCTCTT CCGGGATGAT TGTCTGTATC TACTTCATCA TCCTTTTCAT CTGTGGTAAC 
TATATCCTGC TGAATGTCTT CTTGGCCATC GCTGTGGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA GGAAGCTGAA GAAAAAGAAA GGAAAAAGAT TGCCAGAAAA 
GAAAGCCTAG AAAACAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA TTATCAGGAA GATGCTGAAG ACAAGGACCC TTACCCGCCC 
TGTGATGTGC CAGTAGGTGA AGAGGAAGAG GAGGAGGAAG AGGATGAGCC CGAAGTTCCT 
GCTGGTCCCC GCCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAGAT TGCGCCCATC 
CCAGAAGGAA GCGCCTTCTT CATTCTTAGC AAGACCAACC CGATACGTGT GGGTTGCCAC 
AAGCTCATTA ACCACCACAT CTTCACCAAC CTTATCCTGG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CTGCAGAGGA CCCCATCCGT AGCCACTCAT TCCGCAACAC GATACTGGGC 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACGGTTGAAA TCCTGTTAAA GATGACAACT 
TTTGGAGCCT TCTTGCACAA AGGGGCTTTC TGCAGGAACT ACTTCAATTT GCTGGACATG 
CTGGTTGTTG GGGTGTCGCT GGTGTCCTTT GGGATTCAGT CCAGCGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTTTTGAG GGTCTTGCGG CCTCTCAGAG CAATCAACAG AGCAAAGGGA 
CTTAAGCACG TGGTCCAGTG TGTCTTTGTG GCCATCCGAA CCATTGGCAA CATCATGATC 
GTCACGACCC TGCTCCAGTT CATGTTTGCA TGCATTGGGG TCCAGCTGTT CAAGGGGAAG 
TTCTACCGTT GCACAGATGA AGCCAAAAGT AACCCTGAGG AGTGCAGGGG GCTTTTCATT 
CTTTATAAGG ACGGCGATGT TGACAGTCCT GTGGTCCGTG AGAGGATCTG GCAAAATAGT 
GATTTCAATT TTGACAATGT CCTTTCGGCT ATGATGGCGC TGTTCACAGT CTCAACTTTT 
GAGGGCTGGC CCGCGTTGCT GTACAAAGCT ATTGATTCAA ACGGTGAGAA CGTTGGTCCT 
GTCTACAACT ACCGTGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTTGCC 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CCTTCCAGGA ACAGGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATATGCCTTG 
AAAGCACGCC CCTTAAGGAG ATACATCCCC AAAAACCCAT ACCAGTACAA GTTCTGGTAT 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTCGTCC TCATCATGCT CAACACGCTC 
TGCCTGGCCA TGCAGCACTA TGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACAGGGGT CTTCACTGTT GAGATGGTTT TGAAAGTCAT TGCTTTCAAG 
CCCAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAC TGCTGTGGAC CTTCATCAAG TCCTTTCAGG CGCTGCCGTA TGTTGCCCTC 
CTCATTGCCA TGCTCTTCTT CATCTACGCT GTCATAGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAACAACT TCCAGACCTT TCCTCAGGCA 
GTGCTCCTAC TCTTCAGGTG TGCAACAGGG GAAGCCTGGC AGGAGATCAT GCTTGCCTGC 
CTCCCAGGAA AGCTGTGCGA CCCAGACTCC GATTACAACC CAGGAGAGGA GTACACTTGT 
GGGAGCAACT TTGCCATTGT CTACTTCATC AGCTTTTACA TGCTCTGTGC ATTCCTGATC 
ATCAACCTCT TTGTGGCTGT CATCATGGAC AATTTTGACT ATCTGACTCG GGACTGGTCT 
ATTCTGGGGC CTCACCACTT GGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAA 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TCCGGCGGAT CCAGCCTCCC 
CTGGGGTTTG GAAAATTATG CCCACACCGA GTAGCATGTA AGAGATTGGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTCAATGCAA CCCTGTTTGC TTTGGTCCGG 
ACAGCTCTCA AGATCAAGAC TGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTCCGAGCT 
GTGATCAAGA AAATCTGGAA GAAGACAAGC ATGAAGCTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAGCAA GGACTGGTGG GGAAGTACCC TGCGAAGAAC 
ACCACGATTG CCCTACAGGC GGGATTAAGG ACCCTGCATG ACATTGGGCC AGAAATCCGA 
CGGGCTATAT CCTGTGACTT GCAAGATGAC GAGCCAGAAG ACTCCAAACC AGAAGAAGAA 
GATGTATTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATGCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGGG 
TGTCATAACC ATCATAACCA TAATTCCATA GGGAAGCAAG CTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCACGGAA AGCCGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATTAC TCCTGCAAGC ATGATCGAGA GCTTCAAAGA 
AGGTCCAGTA TTAAAAGGTC TGAGTCAGGA GATGAGCAAT TTCCAACCAT TTGCCGTGAA 
GATCCTGAGA TACACGGCTA CTTCCGAGAC CCCCGCTGCT TGGGGGAACA GGAGTATTTC 
AGTAGCGAGG AGTGCTGTGA GGACGACAGC TCTCCCACCT GGAGCAGGCA AAACTACAAC 
TACTACAACA GGTACCCAGG CAGCTCCATG GACTTTGAGA GGCCCCGAGG CTACCACCAC 
CCCCAAGGCT TCTTAGAAGA TGATGACTCT CCCACTGGCT ATGATTCACG GAGATCTCCG 
AGGAGACGTC TTCTACCTCC TACCCCACCA TCCCATCGGA GGTCCTCCTT CAACTTTGAG 
TGTCTGCGCA GGCAAAGCAG CCAAGACGAT GTCCTTCCAT CGCCTGCCCT GCCTCACCGC 
GCTGCCCTGC CCCTGCACCT GATGCAGCAG CAGATCATGG CCGTTGCAGG CCTAGATTCC 
AGTAAAGCCC AGAAGTACTC ACCGAGCCAC TCTACCCGGT CGTGGGCCAC CCCTCCAGCA 
ACCCCTCCAT ACCGAGACTG GAGCCCATGC TACACCCCTT TGATCCAGGT GGACCGCTCA 
GAATCTATGG ACCAGGTCAA TGGCAGCCTG CCATCCCTAC ACCGAAGCTC CTGGTACACA 
GATGAACCCG ACATCTCCTA TAGGACTTTC ACGCCAGCTA GTCTGACTGT CCCCAGCAGC 
TTCCGGAACA AGAACAGCGA CAAGCAGAGG AGTGCAGACA GCCTGGTGGA GGCAGTCCTG 
ATATCCGAAG GCTTGGGACG CTACGCGAGG GACCCCAAAT TTGTCTCAGC GACAAAACAT 
GAGATTGCCG ATGCCTGTGA CCTAACCATA GACGAGATGG AAAGTGCTGC CAGCACCCTG 
CTCAATGGCA GTGTGTGTCC TCGAGCCAAT GGGGACATGG GCCCCATCTC CCACCGCCAG 
GACTATGAGC TCCAGGACTT TGGTCCTGGC TACAGTGACG AGGAGCCAGA CCCTGGGCGA 
GAGGAAGAGG ACCTGGCAGA TGAGATGATT TGCATTACCA CCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cav1.3 and Cav1.2 channels of adrenal chromaffin cells: emerging views on cAMP/cGMP-mediated phosphorylation and role in pacemaking.
Biochimica et biophysica acta1828(7)1608-18(2013 Jul)
Vandael DH,Mahapatra S,Calorio C,Marcantoni A,Carbone E

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Cacna1d cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: