rpl28 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rpl28
Entrez Gene ID	394036
Full Name	ribosomal protein L28
Synonyms	rpl28l,zgc:66289
General protein information	Preferred Names ribosomal protein L28 Names 60S ribosomal protein L28
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201061.2	NP_957355.1	60S ribosomal protein L28
XM_021466651.1	XP_021322326.1	60S ribosomal protein L28 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rpl28	NP_033107.1
Rattus norvegicus (Norway rat)	Rpl28	NP_073188.2
	RPL28	XP_001087214.1
Canis lupus familiaris (dog)	RPL28	XP_533581.3
Drosophila melanogaster (fruit fly)	RpL28	NP_001163336.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005427	XP_315433.4
Schizosaccharomyces pombe (fission yeast)	rpl44	NP_593124.1
Xenopus tropicalis (tropical clawed frog)	rpl28	NP_001016714.1
Magnaporthe oryzae (rice blast fungus)	MGG_08834	XP_003713853.1
Neurospora crassa	NCU06210	XP_962926.1
Bos taurus (cattle)	RPL28	NP_001030580.1
Danio rerio (zebrafish)	rpl28	NP_957355.1
Homo sapiens (human)	RPL28	NP_000982.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

On the crucial cerebellar wound healing-related pathways and their cross-talks after traumatic brain injury in Danio rerio.
Wu CC, Tsai TH, Chang C, Lee TT, Lin C, Cheng IH, Sun MC, Chuang YJ, Chen BS
PloS one9(6)e97902(2014)

Multiple ribosomal proteins are expressed at high levels in developing zebrafish endoderm and are required for normal exocrine pancreas development.
Provost E, Weier CA, Leach SD
Zebrafish10(2)161-9(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rpl28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rpl28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31449	NM_201061.2 Latest version!	Danio rerio ribosomal protein L28 (rpl28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa149218	XM_021466651.1 Latest version!	Danio rerio ribosomal protein L28 (rpl28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rpl28 ( NM_201061.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957355.1 Danio rerio ribosomal protein L28 (rpl28), mRNA.

Price & Availability↑

CloneID	ODa31449
Clone ID Related Accession (Same CDS sequence)	NM_201061.2
Accession Version	NM_201061.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	60S ribosomal protein L28
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC055584.1. On Aug 31, 2012 this sequence version replaced NM_201061.1. ##Evidence-Data-START## Transcript exon combination :: BC055584.1, CK400894.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCGC CTCACCTGCA ATGGATGGTC ATCAGAAACA GCTCCAGTTT TCTCATCAAG 
AGGAACAAAC AGACCTACAG CACTGAGCCG AATAACCTGA AGGCCAGGAA TGCTTTCCGC 
TTCAATGGTC TCATTCACAG GAAGACCATT GGCATTGAGC CGGCCGCTGA CGGCAAAGGT 
GTGGTCGTCG TCCTGAAGAA ACGTTCAGGT CAGCGCAAAC CTGCCACCTC ATATGGGAAG 
ATCACCATCA ACAAGAACTC TCGCGCCACC CTCGCCAGCG TCAGGAACAT CATCCGCAAA 
AACAAATACA GGAGAGATCT GCGTATGGCT GCTCTGAGAC GCGTGAGCGC CATCCTCAGC 
AGTCAGAAGC CTGTGGTGGT GAGGAAGAAG CGCTCCAGGG CTCCCAAGAG TTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957355.1
CDS	39..455
Translation	MASPHLQWMVIRNSSSFLIKRNKQTYSTEPNNLKARNAFRFNGLIHRKTIGIEPAADGKGVVVVLKKRSGQRKPATSYGKITINKNSRATLASVRNIIRKNKYRRDLRMAALRRVSAILSSQKPVVVRKKRSRAPKSS

Target ORF information:

RefSeq Version	NM_201061.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ribosomal protein L28 (rpl28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201061.2

ORF Insert Sequence:

ATGGCATCGC CTCACCTGCA ATGGATGGTC ATCAGAAACA GCTCCAGTTT TCTCATCAAG 
AGGAACAAAC AGACCTACAG CACTGAGCCG AATAACCTGA AGGCCAGGAA TGCTTTCCGC 
TTCAATGGTC TCATTCACAG GAAGACCATT GGCATTGAGC CGGCCGCTGA CGGCAAAGGT 
GTGGTCGTCG TCCTGAAGAA ACGTTCAGGT CAGCGCAAAC CTGCCACCTC ATATGGGAAG 
ATCACCATCA ACAAGAACTC TCGCGCCACC CTCGCCAGCG TCAGGAACAT CATCCGCAAA 
AACAAATACA GGAGAGATCT GCGTATGGCT GCTCTGAGAC GCGTGAGCGC CATCCTCAGC 
AGTCAGAAGC CTGTGGTGGT GAGGAAGAAG CGCTCCAGGG CTCCCAAGAG TTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rpl28 ( XM_021466651.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322326.1 Danio rerio ribosomal protein L28 (rpl28), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa149218
Clone ID Related Accession (Same CDS sequence)	XM_021466651.1
Accession Version	XM_021466651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	60S ribosomal protein L28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCGC CTCACCTGCA GTGGATGGTC ATCAGAAACA GCTCTAGTTT TCTCATCAAG 
AGGAACAAAC AGACCTACAG CACCGAGCCG AATAACCTGA AGGCCAGGAA TGCTTTCCGC 
TTCAATGGTC TCATTCACAG GAAGACCATT GGCATTGAGC CGGCCGCTGA CGGCAAAGGT 
GTGGTCGTGG TCCTGAAGAA ACGTTCAGGT CAGCGCAAAC CTGCCTCCTC ATATGGGAAG 
ATCACCATCA ACAAGAACTC TCGCGCCACC CTCGCCAGCG TCAGGAACAT CATCCGCAAA 
AACAAATACA GGAGAGATCT GCGTATGGCT GCTCTGAGAC GCGTGAGCGC CATCCTCAGC 
AGTCAGAAGC CTGTGGTGGT GAGGAAGAAG CGCTCCAGGG CTCCCAAGAG TTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322326.1
CDS	114..530
Translation	MASPHLQWMVIRNSSSFLIKRNKQTYSTEPNNLKARNAFRFNGLIHRKTIGIEPAADGKGVVVVLKKRSGQRKPASSYGKITINKNSRATLASVRNIIRKNKYRRDLRMAALRRVSAILSSQKPVVVRKKRSRAPKSS

Target ORF information:

RefSeq Version	XM_021466651.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ribosomal protein L28 (rpl28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466651.1

ORF Insert Sequence:

ATGGCATCGC CTCACCTGCA GTGGATGGTC ATCAGAAACA GCTCTAGTTT TCTCATCAAG 
AGGAACAAAC AGACCTACAG CACCGAGCCG AATAACCTGA AGGCCAGGAA TGCTTTCCGC 
TTCAATGGTC TCATTCACAG GAAGACCATT GGCATTGAGC CGGCCGCTGA CGGCAAAGGT 
GTGGTCGTGG TCCTGAAGAA ACGTTCAGGT CAGCGCAAAC CTGCCTCCTC ATATGGGAAG 
ATCACCATCA ACAAGAACTC TCGCGCCACC CTCGCCAGCG TCAGGAACAT CATCCGCAAA 
AACAAATACA GGAGAGATCT GCGTATGGCT GCTCTGAGAC GCGTGAGCGC CATCCTCAGC 
AGTCAGAAGC CTGTGGTGGT GAGGAAGAAG CGCTCCAGGG CTCCCAAGAG TTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

On the crucial cerebellar wound healing-related pathways and their cross-talks after traumatic brain injury in Danio rerio.
PloS one9(6)e97902(2014)
Wu CC,Tsai TH,Chang C,Lee TT,Lin C,Cheng IH,Sun MC,Chuang YJ,Chen BS

Multiple ribosomal proteins are expressed at high levels in developing zebrafish endoderm and are required for normal exocrine pancreas development.
Zebrafish10(2)161-9(2013 Jun)
Provost E,Weier CA,Leach SD