RPL28 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPL28
Entrez Gene ID	6158
Full Name	ribosomal protein L28
Synonyms	L28
General protein information	Preferred Names ribosomal protein L28 Names 60S ribosomal protein L28 large ribosomal subunit protein eL28
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.42
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008].
Disorder MIM:	603638

mRNA and Protein(s)

mRNA	Protein	Name
NM_001136137.1	NP_001129609.1	60S ribosomal protein L28 isoform 5
NM_001136136.1	NP_001129608.1	60S ribosomal protein L28 isoform 4
NM_001363697.1	NP_001350626.1	60S ribosomal protein L28 isoform 6
NM_001136135.2	NP_001129607.1	60S ribosomal protein L28 isoform 3
NM_000991.4	NP_000982.2	60S ribosomal protein L28 isoform 2
NM_001136134.1	NP_001129606.1	60S ribosomal protein L28 isoform 1
NM_001136135.1	NP_001129607.1	60S ribosomal protein L28 isoform 3
NM_000991.5	NP_000982.2	60S ribosomal protein L28 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rpl28	NP_033107.1
Rattus norvegicus (Norway rat)	Rpl28	NP_073188.2
	RPL28	XP_001087214.1
Canis lupus familiaris (dog)	RPL28	XP_533581.3
Drosophila melanogaster (fruit fly)	RpL28	NP_001163336.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005427	XP_315433.4
Schizosaccharomyces pombe (fission yeast)	rpl44	NP_593124.1
Xenopus tropicalis (tropical clawed frog)	rpl28	NP_001016714.1
Magnaporthe oryzae (rice blast fungus)	MGG_08834	XP_003713853.1
Neurospora crassa	NCU06210	XP_962926.1
Bos taurus (cattle)	RPL28	NP_001030580.1
Danio rerio (zebrafish)	rpl28	NP_957355.1
Homo sapiens (human)	RPL28	NP_000982.2

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Identification of Novel Tau Interactions with Endoplasmic Reticulum Proteins in Alzheimer's Disease Brain.
Meier S, Bell M, Lyons DN, Ingram A, Chen J, Gensel JC, Zhu H, Nelson PT, Abisambra JF
Journal of Alzheimer's disease : JAD48(3)687-702(2015)

Genome-wide RNAi screen identifies the Parkinson disease GWAS risk locus SREBF1 as a regulator of mitophagy.
Ivatt RM, Sanchez-Martinez A, Godena VK, Brown S, Ziviani E, Whitworth AJ
Proceedings of the National Academy of Sciences of the United States of America111(23)8494-9(2014 Jun)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging.

The following RPL28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPL28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31352	NM_000991.5 Latest version!	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31549	NM_001136137.1 Latest version!	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu07405	NM_001136135.2 Latest version!	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu30619	NM_001136134.1 Latest version!	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu31657	NM_001136136.1 Latest version!	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu52416	NM_001363697.1 Latest version!	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu07405	NM_001136135.1	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu31352	NM_000991.4	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPL28 ( NM_000991.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000982.2 Homo sapiens ribosomal protein L28 (RPL28), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu31352
Clone ID Related Accession (Same CDS sequence)	NM_000991.4 , NM_000991.5
Accession Version	NM_000991.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	60S ribosomal protein L28 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AK311760.1, AC020922.9 and BC011582.1. On Oct 23, 2008 this sequence version replaced NM_000991.3. Transcript Variant: This variant (2) lacks a stop-codon-containing segment in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) has a distinct and shorter C-terminus when compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic and transcript sequence to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011582.1, SRR1803614.86365.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGGCCAG CGGAAGCCTG CCACCTCCTA TGTGCGGACC 
ACCATCAACA AGAATGCTCG CGCCACGCTC AGCAGCATCA GACACATGAT CCGCAAGAAC 
AAGTACCGCC CCGACCTGCG CATGGCAGCC ATCCGCAGGG CCAGCGCCAT CCTGCGCAGC 
CAGAAGCCTG TGATGGTGAA GAGGAAGCGG ACCCGCCCCA CCAAGAGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000982.2
CDS	43..456
Translation	MSAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKGVVVVIKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMAAIRRASAILRSQKPVMVKRKRTRPTKSS

Target ORF information:

RefSeq Version	NM_000991.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000991.4

ORF Insert Sequence:

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGGCCAG CGGAAGCCTG CCACCTCCTA TGTGCGGACC 
ACCATCAACA AGAATGCTCG CGCCACGCTC AGCAGCATCA GACACATGAT CCGCAAGAAC 
AAGTACCGCC CCGACCTGCG CATGGCAGCC ATCCGCAGGG CCAGCGCCAT CCTGCGCAGC 
CAGAAGCCTG TGATGGTGAA GAGGAAGCGG ACCCGCCCCA CCAAGAGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL28 ( NM_000991.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000982.2 Homo sapiens ribosomal protein L28 (RPL28), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu31352
Clone ID Related Accession (Same CDS sequence)	NM_000991.4 , NM_000991.5
Accession Version	NM_000991.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	60S ribosomal protein L28 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AK311760.1, AC020922.9 and BC011582.1. On Jun 2, 2019 this sequence version replaced NM_000991.4. Transcript Variant: This variant (2) lacks a stop-codon-containing segment in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) has a distinct and shorter C-terminus when compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic and transcript sequence to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011582.1, AU131942.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGGCCAG CGGAAGCCTG CCACCTCCTA TGTGCGGACC 
ACCATCAACA AGAATGCTCG CGCCACGCTC AGCAGCATCA GACACATGAT CCGCAAGAAC 
AAGTACCGCC CCGACCTGCG CATGGCAGCC ATCCGCAGGG CCAGCGCCAT CCTGCGCAGC 
CAGAAGCCTG TGATGGTGAA GAGGAAGCGG ACCCGCCCCA CCAAGAGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000982.2
CDS	43..456
Translation	MSAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKGVVVVIKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMAAIRRASAILRSQKPVMVKRKRTRPTKSS

Target ORF information:

RefSeq Version	NM_000991.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000991.5

ORF Insert Sequence:

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGGCCAG CGGAAGCCTG CCACCTCCTA TGTGCGGACC 
ACCATCAACA AGAATGCTCG CGCCACGCTC AGCAGCATCA GACACATGAT CCGCAAGAAC 
AAGTACCGCC CCGACCTGCG CATGGCAGCC ATCCGCAGGG CCAGCGCCAT CCTGCGCAGC 
CAGAAGCCTG TGATGGTGAA GAGGAAGCGG ACCCGCCCCA CCAAGAGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL28 ( NM_001136137.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129609.1 Homo sapiens ribosomal protein L28 (RPL28), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu31549
Clone ID Related Accession (Same CDS sequence)	NM_001136137.1
Accession Version	NM_001136137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	60S ribosomal protein L28 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA092122.1 and BM702093.1. Transcript Variant: This variant (5) differs in the 3' UTR and has multiple coding region differences, compared to variant 1. The encoded protein (isoform 5) has a distinct and shorter C-terminus when compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CF128099.1, BI333175.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129609.1
CDS	43..252
Translation	MSAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKGVVVVIKRRSE

Target ORF information:

RefSeq Version	NM_001136137.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136137.1

ORF Insert Sequence:

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL28 ( NM_001136135.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129607.1 Homo sapiens ribosomal protein L28 (RPL28), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07405
Clone ID Related Accession (Same CDS sequence)	NM_001136135.2 , NM_001136135.1
Accession Version	NM_001136135.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	60S ribosomal protein L28 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP227047.1, AC020922.9 and BC011582.1. On Jun 2, 2019 this sequence version replaced NM_001136135.1. Transcript Variant: This variant (3) lacks a stop-codon-containing segment in the 3' coding region, compared to variant 1. The encoded protein (isoform 3) has a distinct and longer C-terminus when compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic and transcript sequence to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.574282.1, SRR7346977.208999.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGGCCAG CGGAAGCCTG CCACCTCCTA TGTGCGGACC 
ACCATCAACA AGAATGCTCG CGCCACGCTC AGCAGCATCA GACACATGAT CCGCAAGAAC 
AAGTACCGCC CCGACCTGCG CATGGACATG CTGGCATCTA CTGGGTCAGG GCTCTGCTGC 
TCGGTGGCTG TGCAACCTTG GGCAAGTTCC TCAACCTCTC TGTGTCTTCG TACCCTCATC 
TGTAACATGC GTGTCGATAG ACCCTACTAC TCAGGGTTGA TGAGAAGATT AAATGTGCAA 
AACCTGCTTG ACTGTGCCCA CAAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129607.1
CDS	43..552
Translation	MSAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKGVVVVIKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMDMLASTGSGLCCSVAVQPWASSSTSLCLRTLICNMRVDRPYYSGLMRRLNVQNLLDCAHKS

Target ORF information:

RefSeq Version	NM_001136135.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136135.2

ORF Insert Sequence:

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGGCCAG CGGAAGCCTG CCACCTCCTA TGTGCGGACC 
ACCATCAACA AGAATGCTCG CGCCACGCTC AGCAGCATCA GACACATGAT CCGCAAGAAC 
AAGTACCGCC CCGACCTGCG CATGGACATG CTGGCATCTA CTGGGTCAGG GCTCTGCTGC 
TCGGTGGCTG TGCAACCTTG GGCAAGTTCC TCAACCTCTC TGTGTCTTCG TACCCTCATC 
TGTAACATGC GTGTCGATAG ACCCTACTAC TCAGGGTTGA TGAGAAGATT AAATGTGCAA 
AACCTGCTTG ACTGTGCCCA CAAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL28 ( NM_001136135.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129607.1 Homo sapiens ribosomal protein L28 (RPL28), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07405
Clone ID Related Accession (Same CDS sequence)	NM_001136135.2 , NM_001136135.1
Accession Version	NM_001136135.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-05
Organism	Homo sapiens(human)
Product	60S ribosomal protein L28 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP227047.1, AC020922.9 and BC011582.1. Transcript Variant: This variant (3) lacks a stop-codon-containing segment in the 3' coding region, compared to variant 1. The encoded protein (isoform 3) has a distinct and longer C-terminus when compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic and transcript sequence to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.312645.1, SRR1163655.542874.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGGCCAG CGGAAGCCTG CCACCTCCTA TGTGCGGACC 
ACCATCAACA AGAATGCTCG CGCCACGCTC AGCAGCATCA GACACATGAT CCGCAAGAAC 
AAGTACCGCC CCGACCTGCG CATGGACATG CTGGCATCTA CTGGGTCAGG GCTCTGCTGC 
TCGGTGGCTG TGCAACCTTG GGCAAGTTCC TCAACCTCTC TGTGTCTTCG TACCCTCATC 
TGTAACATGC GTGTCGATAG ACCCTACTAC TCAGGGTTGA TGAGAAGATT AAATGTGCAA 
AACCTGCTTG ACTGTGCCCA CAAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129607.1
CDS	43..552
Translation	MSAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKGVVVVIKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMDMLASTGSGLCCSVAVQPWASSSTSLCLRTLICNMRVDRPYYSGLMRRLNVQNLLDCAHKS

Target ORF information:

RefSeq Version	NM_001136135.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136135.1

ORF Insert Sequence:

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGGCCAG CGGAAGCCTG CCACCTCCTA TGTGCGGACC 
ACCATCAACA AGAATGCTCG CGCCACGCTC AGCAGCATCA GACACATGAT CCGCAAGAAC 
AAGTACCGCC CCGACCTGCG CATGGACATG CTGGCATCTA CTGGGTCAGG GCTCTGCTGC 
TCGGTGGCTG TGCAACCTTG GGCAAGTTCC TCAACCTCTC TGTGTCTTCG TACCCTCATC 
TGTAACATGC GTGTCGATAG ACCCTACTAC TCAGGGTTGA TGAGAAGATT AAATGTGCAA 
AACCTGCTTG ACTGTGCCCA CAAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL28 ( NM_001136134.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129606.1 Homo sapiens ribosomal protein L28 (RPL28), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30619
Clone ID Related Accession (Same CDS sequence)	NM_001136134.1
Accession Version	NM_001136134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	60S ribosomal protein L28 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020922.9, AK293736.1 and BC011582.1. Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic and transcript sequence to make the sequence consistent with the reference genome assembly. The extent of this transcript is based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DC400649.1, AK293736.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGGCCAG CGGAAGCCTG CCACCTCCTA TGTGCGGACC 
ACCATCAACA AGAATGCTCG CGCCACGCTC AGCAGCATCA GACACATGAT CCGCAAGAAC 
AAGTACCGCC CCGACCTGCG CATGGTGAGC TGGGGTTTGG GGATCAGGCT TGGGGAGACT 
GGCCAGTGCT GTGGGGAAGG GCCTCCCACT ACTGGTTGCA ATATGGGCTG GAGAGGGATG 
GATTCTTGCT TTCAGCCTAC TCCCCACACC CAGCATTGGC CTAGGGGGCG GCTTGTGGAG 
TGTATGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129606.1
CDS	43..534
Translation	MSAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKGVVVVIKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMVSWGLGIRLGETGQCCGEGPPTTGCNMGWRGMDSCFQPTPHTQHWPRGRLVECMG

Target ORF information:

RefSeq Version	NM_001136134.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136134.1

ORF Insert Sequence:

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGGCCAG CGGAAGCCTG CCACCTCCTA TGTGCGGACC 
ACCATCAACA AGAATGCTCG CGCCACGCTC AGCAGCATCA GACACATGAT CCGCAAGAAC 
AAGTACCGCC CCGACCTGCG CATGGTGAGC TGGGGTTTGG GGATCAGGCT TGGGGAGACT 
GGCCAGTGCT GTGGGGAAGG GCCTCCCACT ACTGGTTGCA ATATGGGCTG GAGAGGGATG 
GATTCTTGCT TTCAGCCTAC TCCCCACACC CAGCATTGGC CTAGGGGGCG GCTTGTGGAG 
TGTATGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL28 ( NM_001136136.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129608.1 Homo sapiens ribosomal protein L28 (RPL28), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu31657
Clone ID Related Accession (Same CDS sequence)	NM_001136136.1
Accession Version	NM_001136136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	60S ribosomal protein L28 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA820669.1 and DA333143.1. Transcript Variant: This variant (4) differs in the 3' UTR and has multiple coding region differences, compared to variant 1. The encoded protein (isoform 4) has a distinct and shorter C-terminus when compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.383096.1, SRR1660805.294481.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGGTGAG TTTTGTCTGG TTTGGGCCAG AGAGCGGCCC 
CTTTCCCGGG TCTGGGAGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129608.1
CDS	43..306
Translation	MSAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKGVVVVIKRRSGEFCLVWARERPLSRVWEL

Target ORF information:

RefSeq Version	NM_001136136.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136136.1

ORF Insert Sequence:

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGGTGAG TTTTGTCTGG TTTGGGCCAG AGAGCGGCCC 
CTTTCCCGGG TCTGGGAGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL28 ( NM_001363697.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350626.1 Homo sapiens ribosomal protein L28 (RPL28), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu52416
Clone ID Related Accession (Same CDS sequence)	NM_001363697.1
Accession Version	NM_001363697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	60S ribosomal protein L28 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK311760.1, BG716117.1, BF686026.1, DB540276.1 and AA579944.1. On May 25, 2018 this sequence version replaced XM_024451636.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG716117.1, DB471544.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGGCCAG CGGAAGCCTG CCACCTCCTA TGTGCGGACC 
ACCATCAACA AGAATGCTCG CGCCACGCTC AGCAGCATCA GACACATGAT CCGCAAGAAC 
AAGTACCGCC CCGACCTGCG CATGCTTGCG GGAAGGGTTG GGAGGCAGCA GGCTGTAAGC 
AGCCTGGAGC ACCAGCCTAG ACCAGGACGC CTCCACCTCA GCAACACCGC AGCCAGGTCA 
TTCTGTGTCA TGGAGCCATC TCGTACGCTG CAGGATTTGG GCAGCACCCT TGGCCTCCAC 
CCACTAGATG CTAGTGGCAC CCCCGAGTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350626.1
CDS	43..555
Translation	MSAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKGVVVVIKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMLAGRVGRQQAVSSLEHQPRPGRLHLSNTAARSFCVMEPSRTLQDLGSTLGLHPLDASGTPEL

Target ORF information:

RefSeq Version	NM_001363697.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L28 (RPL28), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363697.1

ORF Insert Sequence:

ATGTCTGCGC ATCTGCAATG GATGGTCGTG CGGAACTGCT CCAGTTTCCT GATCAAGAGG 
AATAAGCAGA CCTACAGCAC TGAGCCCAAT AACTTGAAGG CCCGCAATTC CTTCCGCTAC 
AACGGACTGA TTCACCGCAA GACTGTGGGC GTGGAGCCGG CAGCCGACGG CAAAGGTGTC 
GTGGTGGTCA TTAAGCGGAG ATCCGGCCAG CGGAAGCCTG CCACCTCCTA TGTGCGGACC 
ACCATCAACA AGAATGCTCG CGCCACGCTC AGCAGCATCA GACACATGAT CCGCAAGAAC 
AAGTACCGCC CCGACCTGCG CATGCTTGCG GGAAGGGTTG GGAGGCAGCA GGCTGTAAGC 
AGCCTGGAGC ACCAGCCTAG ACCAGGACGC CTCCACCTCA GCAACACCGC AGCCAGGTCA 
TTCTGTGTCA TGGAGCCATC TCGTACGCTG CAGGATTTGG GCAGCACCCT TGGCCTCCAC 
CCACTAGATG CTAGTGGCAC CCCCGAGTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Identification of Novel Tau Interactions with Endoplasmic Reticulum Proteins in Alzheimer's Disease Brain.
Journal of Alzheimer's disease : JAD48(3)687-702(2015)
Meier S,Bell M,Lyons DN,Ingram A,Chen J,Gensel JC,Zhu H,Nelson PT,Abisambra JF

Genome-wide RNAi screen identifies the Parkinson disease GWAS risk locus SREBF1 as a regulator of mitophagy.
Proceedings of the National Academy of Sciences of the United States of America111(23)8494-9(2014 Jun)
Ivatt RM,Sanchez-Martinez A,Godena VK,Brown S,Ziviani E,Whitworth AJ

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

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RPL28 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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