ANKRD27 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKRD27
Entrez Gene ID	386887
Full Name	ankyrin repeat domain 27 (VPS9 domain)
Synonyms	fc41e04,si:ch211-113a14.30,wu:fc41e04
General protein information	Preferred Names ankyrin repeat domain 27 (VPS9 domain) Names ankyrin repeat domain-containing protein 27
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021470748.1	XP_021326423.1	ankyrin repeat domain-containing protein 27 isoform X3
XM_021470749.1	XP_021326424.1	ankyrin repeat domain-containing protein 27 isoform X4
XM_002666959.5	XP_002667005.4	ankyrin repeat domain-containing protein 27 isoform X2
XM_021470747.1	XP_021326422.1	ankyrin repeat domain-containing protein 27 isoform X3
XM_017354281.1	XP_017209770.1	ankyrin repeat domain-containing protein 27 isoform X1
XM_021470745.1	XP_021326420.1	ankyrin repeat domain-containing protein 27 isoform X1
XM_021470746.1	XP_021326421.1	ankyrin repeat domain-containing protein 27 isoform X2
XM_017354282.1	XP_017209771.1	ankyrin repeat domain-containing protein 27 isoform X2

Pathways from BioSystems

Homologies

	LOC716232	XP_001106852.1
Rattus norvegicus (Norway rat)	Ankrd27	NP_001258193.1
Pan troglodytes (chimpanzee)	ANKRD27	XP_001151730.1
Bos taurus (cattle)	ANKRD27	NP_001096002.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003151	XP_001688409.2
Homo sapiens (human)	ANKRD27	NP_115515.2
Danio rerio (zebrafish)	wu:fc41e04	XP_005163257.1
Xenopus tropicalis (tropical clawed frog)	ankrd27	XP_004913610.1
Canis lupus familiaris (dog)	ANKRD27	XP_005616956.1
Mus musculus (house mouse)	Ankrd27	NP_663608.3
Gallus gallus (chicken)	ANKRD27	NP_001129150.1

Gene Ontology

The following ANKRD27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKRD27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa141495	XM_021470745.1 Latest version!	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa141496	XM_021470746.1 Latest version!	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa141497	XM_021470747.1 Latest version!	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa141497	XM_021470748.1 Latest version!	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa141498	XM_021470749.1 Latest version!	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa57976	XM_017354281.1 Latest version!	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa57977	XM_002666959.5 Latest version!	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa57977	XM_017354282.1 Latest version!	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKRD27 ( XM_021470745.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326420.1 Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa141495
Clone ID Related Accession (Same CDS sequence)	XM_021470745.1
Accession Version	XM_021470745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat domain-containing protein 27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 983 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCGTTCC TCTTCCTCAT TCGTCATTTG ATGGATTTAA AGATGAAATT GTACATGGTG 
TCGAAAACTC TTCTTAACCT TCATAAATAT GAGCTGGAGT TTGGGACACA TATGGCTGTG 
TATGATGAGA ATATCCTGAA AAACCCTTTT TACCAGGCTC TGGAGAAACA CAGACCGGAC 
CTGTGCAGCC GGGTGGCAGA GCTCCATGGC ATTATCCTGG TACCGTGTTG TGGAAGTTTA 
CCAACGGGCT CTTTTACTGC AGCTCAGTTT GACGGATACG TTCTTCAACC TTCCGAGCAG 
GGTTACCAGA CTGTAGATGG AAAGGAAGTG TCTATAAACA ATAACCGGGT CCACCTGAGT 
GCAGGGTTGC CCAATCCTAT CTCTGTCCCC ATCCTGTTTG AGGAGACCTT CTACAATGAG 
AAAGAGCAGG CCTACAGTAT ACTGTGCATC GCACGGCCCT TCGAATCTGA CCACATTCCT 
GCTGAGGAGT TGGGTTCTGT TCCTGCTCCG TACTGTCTGA AAAATATTGA GGATGTGAGG 
GAGTTTTTGG GCCGCCATGC TGAAAAACTG GACAAGTTTG TTTCCTCCTT CTGTCTCTCC 
TTTAAAGAAC AGGAGAGGAA AGGCCTACGC CATCACATAG ACTCGGTGAA TACGCTTTAC 
ACCAGATGTC TTCAGTGTCT GCTGAGAGAC TCTCGTCTGA AGCTTTTGGC GAGACAGGAG 
ATTCAGATGA CTCTACTCAA AGAAGCAGTA GAGATGTACA TCCATCATGG CATCCATGAT 
CTACTCTTTA GTTATGTGGG CACTCTTGAA GCTAGTCGGG ATGCAGCGTT CAACAAAACA 
ACACGGTCCT TGCAGGATCT GCAGCAGAAA GAGCTGGGAG TGAAGTCCGA GTTCAGTATT 
AATATTCCTC GGGCGAAGCG TGAGCTGATT CAGCTGAACG TCTGCACGTC TCCACAGCAG 
AAACTCCTCT GCATCCGGAA AGTCATTTTA ACCATCATGC AGTCGACCAG ACGCAGAGAT 
ACAGCGGTGA GCATCGAGTC CATGTGTGCC GATGATCTGC TGTTGGTCAT TCTGTATCTG 
CTCATCAAGA CAGAAATTCC CAACTGGATG GCCAATCTGA GTTATATAAA GCACTTCCGC 
TTTCGGAGCT CCAGCAAGGA TGAGTTGAGT TACTGTCTGA CGTCTTTCGA GGCCGCGGTC 
GAGTATATCA GCCTGGGAAA CCTCAAACAC AACACCGATG GGTCAGGAAA CAGACTGTCC 
TTCAGGCAGA AGGTGGACCT ACTGTCCCAG AATGCAACAC CCATTGACCG GCTGTTTGAG 
CATATTGTGA GCGGAAATGA GCAGGAGGTT CAGCGTTTGC TCAGTGAAAG CGAGAGTGAG 
GAGGACGGCA GGAAGTTGTG TCACCCTCTG TGTTCCTGTG ACGCCTGCGA CCTGCGCATC 
TCTGGGAGAT TGAATGATCC TTCCATCGTC ACTCCATTCT CTCGAGACGA CAGAGGATAC 
ACTCCTTTAC ATGTTGCTGC TATTTGTGGT CAGTCTCAGA TGATTGATTT ACTGGTGTCA 
AAGGGAGCAG TGGTAAACGC TACCGATTAC CATGCACTCA CACCGCTGCA CCTCTCCTGC 
CAGAAGGGCT ACCAGGGCGT CACGCTTCTC CTGCTGCATT ATAAGGCCAA CACTGATGCT 
CAGGACAACA ATGGAAACAC TCCTCTACAT CTGGCCTGCA TGTACGGACA TGAAGACTGT 
GTGAAGGCGC TGGTGTACTT CGATCTGCAC TGCTGTCGGC TGAACGTTCA GAACGATAAA 
GGAGACTCGC CGCTGCACAT CGCCGCTCGC TGGGGATACG AGGGCATCAT GGAGGTGCTG 
CTGGAGAACG GAGCGTCCAC GCTCATCCAC AATAAAGCCA AGGAGACGCC GCTGCACTGC 
GCGCTCAACT CCAAGATTCT GTCATTGTTG GAGCGCAGAC ACAACGACTC CAGTATAAGG 
GAAAGTGGAT TCGAGTCTCC GAGCCGCTCT CCTCCTCCGT CAGACGGCAG CAGCAGACGC 
TCCTCCATCT CCAGCTCATC CTCTCTGAGT ATTGAAACCT CTCCGCTGGA GTCTGACCGC 
AGCCGGCACA GAGAGGTGGA GAAGCTGTTG AGAGCCGTGG CTGACGGAGA TGTGGAGATG 
GTGCGTTATC TCCTGGAGTG GCTCGATGAG GATCCAGATG AAGAGGAGTC TGCAGTGCCA 
GTCAAGACGG AGCTCTGCCA TCCGCTGTGC CAGTGTGCCA ACTGCGAACC CACACAGAAG 
AAGGCAGCTC ATCTTCATCC AGACAGTGTG GGTGTAAACA GCAGCAGTGT TGACGGCTTC 
ACGCCGCTGC ATGTGGCGGC GCTGCATGGA CACACACCTC TCATCTCTCT GTTCACACGC 
CACGGCGCAA ACCTGAACGC TCGCAACAAC CACAGCGCCA CCCCACTGCA CCTGGCCTGC 
CAGAACAACC ACACAGAGGT GGTCTGTGCT CTCCTGGAGT GTAATGCCAA ACTCAATAAG 
AAGGATCATT ATGGGAACAC ACCGCTCATT CAGGCCTGTC TGAAGGGGAA TCTGGAGATC 
GCCGGCGTCC TGCTGGAGAG CGGTGCGCTG GTAAATCTGG CCAATAACCA CGGGAACACG 
GGTCTGCATG AGGCTGTGAG AGGAGCAAAC GTGTCGCTGG TGGAGCTGCT GCTGCACAGA 
GGAGCTTCAG TTCACATCCG CAACAACAGA CAGAGAACAT CGCTGGACTG CGCACACGAG 
GCCGCCGCCA AGAACTCAGA GATTTTGAGA CTCCTTCAGA AAGCATATGA TGAAGCCCAG 
ACGCAAAGGG CCAGAGCACC AGAGGGCATC ATGGCAAGAG TTAAACAGAG TCCTAACAAT 
GGTGGACAGG ATTTTGAGCA CCACAAACAG AGGAGTCTGA AGAGAACAGA CAGAGCGGCA 
GCTACAACAG CAGCAGCACA ACAGACTGCA GGGAGCGACA GACGGAGACT GACCCGAGGA 
GAAACACTGG ACATCAGTGA TCCACAGCCT CCGAGCAGAC CTCTGACCCG CAGCCACACC 
ATCAGCCAAG ACCTGAGTTC CAGCGTTCAG CCCGAAGAGC AGATCAACGG TGGAAAACGG 
GAGGATGAAG AAGATCCGTC CGGTGCTGAA GCAGATCCTG CAGACACTGA GATGGACAGC 
GTGGTGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326420.1
CDS	50..3241
Translation	MAFLFLIRHLMDLKMKLYMVSKTLLNLHKYELEFGTHMAVYDENILKNPFYQALEKHRPDLCSRVAELHGIILVPCCGSLPTGSFTAAQFDGYVLQPSEQGYQTVDGKEVSINNNRVHLSAGLPNPISVPILFEETFYNEKEQAYSILCIARPFESDHIPAEELGSVPAPYCLKNIEDVREFLGRHAEKLDKFVSSFCLSFKEQERKGLRHHIDSVNTLYTRCLQCLLRDSRLKLLARQEIQMTLLKEAVEMYIHHGIHDLLFSYVGTLEASRDAAFNKTTRSLQDLQQKELGVKSEFSINIPRAKRELIQLNVCTSPQQKLLCIRKVILTIMQSTRRRDTAVSIESMCADDLLLVILYLLIKTEIPNWMANLSYIKHFRFRSSSKDELSYCLTSFEAAVEYISLGNLKHNTDGSGNRLSFRQKVDLLSQNATPIDRLFEHIVSGNEQEVQRLLSESESEEDGRKLCHPLCSCDACDLRISGRLNDPSIVTPFSRDDRGYTPLHVAAICGQSQMIDLLVSKGAVVNATDYHALTPLHLSCQKGYQGVTLLLLHYKANTDAQDNNGNTPLHLACMYGHEDCVKALVYFDLHCCRLNVQNDKGDSPLHIAARWGYEGIMEVLLENGASTLIHNKAKETPLHCALNSKILSLLERRHNDSSIRESGFESPSRSPPPSDGSSRRSSISSSSSLSIETSPLESDRSRHREVEKLLRAVADGDVEMVRYLLEWLDEDPDEEESAVPVKTELCHPLCQCANCEPTQKKAAHLHPDSVGVNSSSVDGFTPLHVAALHGHTPLISLFTRHGANLNARNNHSATPLHLACQNNHTEVVCALLECNAKLNKKDHYGNTPLIQACLKGNLEIAGVLLESGALVNLANNHGNTGLHEAVRGANVSLVELLLHRGASVHIRNNRQRTSLDCAHEAAAKNSEILRLLQKAYDEAQTQRARAPEGIMARVKQSPNNGGQDFEHHKQRSLKRTDRAAATTAAAQQTAGSDRRRLTRGETLDISDPQPPSRPLTRSHTISQDLSSSVQPEEQINGGKREDEEDPSGAEADPADTEMDSVVL

Target ORF information:

RefSeq Version	XM_021470745.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470745.1

ORF Insert Sequence:

ATGGCGTTCC TCTTCCTCAT TCGTCATTTG ATGGATTTAA AGATGAAATT GTACATGGTG 
TCGAAAACTC TTCTTAACCT TCATAAATAT GAGCTGGAGT TTGGGACACA TATGGCTGTG 
TATGATGAGA ATATCCTGAA AAACCCTTTT TACCAGGCTC TGGAGAAACA CAGACCGGAC 
CTGTGCAGCC GGGTGGCAGA GCTCCATGGC ATTATCCTGG TACCGTGTTG TGGAAGTTTA 
CCAACGGGCT CTTTTACTGC AGCTCAGTTT GACGGATACG TTCTTCAACC TTCCGAGCAG 
GGTTACCAGA CTGTAGATGG AAAGGAAGTG TCTATAAACA ATAACCGGGT CCACCTGAGT 
GCAGGGTTGC CCAATCCTAT CTCTGTCCCC ATCCTGTTTG AGGAGACCTT CTACAATGAG 
AAAGAGCAGG CCTACAGTAT ACTGTGCATC GCACGGCCCT TCGAATCTGA CCACATTCCT 
GCTGAGGAGT TGGGTTCTGT TCCTGCTCCG TACTGTCTGA AAAATATTGA GGATGTGAGG 
GAGTTTTTGG GCCGCCATGC TGAAAAACTG GACAAGTTTG TTTCCTCCTT CTGTCTCTCC 
TTTAAAGAAC AGGAGAGGAA AGGCCTACGC CATCACATAG ACTCGGTGAA TACGCTTTAC 
ACCAGATGTC TTCAGTGTCT GCTGAGAGAC TCTCGTCTGA AGCTTTTGGC GAGACAGGAG 
ATTCAGATGA CTCTACTCAA AGAAGCAGTA GAGATGTACA TCCATCATGG CATCCATGAT 
CTACTCTTTA GTTATGTGGG CACTCTTGAA GCTAGTCGGG ATGCAGCGTT CAACAAAACA 
ACACGGTCCT TGCAGGATCT GCAGCAGAAA GAGCTGGGAG TGAAGTCCGA GTTCAGTATT 
AATATTCCTC GGGCGAAGCG TGAGCTGATT CAGCTGAACG TCTGCACGTC TCCACAGCAG 
AAACTCCTCT GCATCCGGAA AGTCATTTTA ACCATCATGC AGTCGACCAG ACGCAGAGAT 
ACAGCGGTGA GCATCGAGTC CATGTGTGCC GATGATCTGC TGTTGGTCAT TCTGTATCTG 
CTCATCAAGA CAGAAATTCC CAACTGGATG GCCAATCTGA GTTATATAAA GCACTTCCGC 
TTTCGGAGCT CCAGCAAGGA TGAGTTGAGT TACTGTCTGA CGTCTTTCGA GGCCGCGGTC 
GAGTATATCA GCCTGGGAAA CCTCAAACAC AACACCGATG GGTCAGGAAA CAGACTGTCC 
TTCAGGCAGA AGGTGGACCT ACTGTCCCAG AATGCAACAC CCATTGACCG GCTGTTTGAG 
CATATTGTGA GCGGAAATGA GCAGGAGGTT CAGCGTTTGC TCAGTGAAAG CGAGAGTGAG 
GAGGACGGCA GGAAGTTGTG TCACCCTCTG TGTTCCTGTG ACGCCTGCGA CCTGCGCATC 
TCTGGGAGAT TGAATGATCC TTCCATCGTC ACTCCATTCT CTCGAGACGA CAGAGGATAC 
ACTCCTTTAC ATGTTGCTGC TATTTGTGGT CAGTCTCAGA TGATTGATTT ACTGGTGTCA 
AAGGGAGCAG TGGTAAACGC TACCGATTAC CATGCACTCA CACCGCTGCA CCTCTCCTGC 
CAGAAGGGCT ACCAGGGCGT CACGCTTCTC CTGCTGCATT ATAAGGCCAA CACTGATGCT 
CAGGACAACA ATGGAAACAC TCCTCTACAT CTGGCCTGCA TGTACGGACA TGAAGACTGT 
GTGAAGGCGC TGGTGTACTT CGATCTGCAC TGCTGTCGGC TGAACGTTCA GAACGATAAA 
GGAGACTCGC CGCTGCACAT CGCCGCTCGC TGGGGATACG AGGGCATCAT GGAGGTGCTG 
CTGGAGAACG GAGCGTCCAC GCTCATCCAC AATAAAGCCA AGGAGACGCC GCTGCACTGC 
GCGCTCAACT CCAAGATTCT GTCATTGTTG GAGCGCAGAC ACAACGACTC CAGTATAAGG 
GAAAGTGGAT TCGAGTCTCC GAGCCGCTCT CCTCCTCCGT CAGACGGCAG CAGCAGACGC 
TCCTCCATCT CCAGCTCATC CTCTCTGAGT ATTGAAACCT CTCCGCTGGA GTCTGACCGC 
AGCCGGCACA GAGAGGTGGA GAAGCTGTTG AGAGCCGTGG CTGACGGAGA TGTGGAGATG 
GTGCGTTATC TCCTGGAGTG GCTCGATGAG GATCCAGATG AAGAGGAGTC TGCAGTGCCA 
GTCAAGACGG AGCTCTGCCA TCCGCTGTGC CAGTGTGCCA ACTGCGAACC CACACAGAAG 
AAGGCAGCTC ATCTTCATCC AGACAGTGTG GGTGTAAACA GCAGCAGTGT TGACGGCTTC 
ACGCCGCTGC ATGTGGCGGC GCTGCATGGA CACACACCTC TCATCTCTCT GTTCACACGC 
CACGGCGCAA ACCTGAACGC TCGCAACAAC CACAGCGCCA CCCCACTGCA CCTGGCCTGC 
CAGAACAACC ACACAGAGGT GGTCTGTGCT CTCCTGGAGT GTAATGCCAA ACTCAATAAG 
AAGGATCATT ATGGGAACAC ACCGCTCATT CAGGCCTGTC TGAAGGGGAA TCTGGAGATC 
GCCGGCGTCC TGCTGGAGAG CGGTGCGCTG GTAAATCTGG CCAATAACCA CGGGAACACG 
GGTCTGCATG AGGCTGTGAG AGGAGCAAAC GTGTCGCTGG TGGAGCTGCT GCTGCACAGA 
GGAGCTTCAG TTCACATCCG CAACAACAGA CAGAGAACAT CGCTGGACTG CGCACACGAG 
GCCGCCGCCA AGAACTCAGA GATTTTGAGA CTCCTTCAGA AAGCATATGA TGAAGCCCAG 
ACGCAAAGGG CCAGAGCACC AGAGGGCATC ATGGCAAGAG TTAAACAGAG TCCTAACAAT 
GGTGGACAGG ATTTTGAGCA CCACAAACAG AGGAGTCTGA AGAGAACAGA CAGAGCGGCA 
GCTACAACAG CAGCAGCACA ACAGACTGCA GGGAGCGACA GACGGAGACT GACCCGAGGA 
GAAACACTGG ACATCAGTGA TCCACAGCCT CCGAGCAGAC CTCTGACCCG CAGCCACACC 
ATCAGCCAAG ACCTGAGTTC CAGCGTTCAG CCCGAAGAGC AGATCAACGG TGGAAAACGG 
GAGGATGAAG AAGATCCGTC CGGTGCTGAA GCAGATCCTG CAGACACTGA GATGGACAGC 
GTGGTGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD27 ( XM_021470746.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326421.1 Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa141496
Clone ID Related Accession (Same CDS sequence)	XM_021470746.1
Accession Version	XM_021470746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat domain-containing protein 27 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 983 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCGTTCC TCTTCCTCAT TCGTCATTTG ATGGATTTAA AGATGAAATT GTACATGGTG 
TCGAAAACTC TTCTTAACCT TCATAAATAT GAGCTGGAGT TTGGGACACA TATGGCTGTG 
TATGATGAGA ATATCCTGAA AAACCCTTTT TACCAGGCTC TGGAGAAACA CAGACCGGAC 
CTGTGCAGCC GGGTGGCAGA GCTCCATGGC ATTATCCTGG TACCGTGTTG TGGAAGTTTA 
CCAACGGGCT CTTTTACTGC AGCTCAGTTT GACGGATACG TTCTTCAACC TTCCGAGCAG 
GGTTACCAGA CTGTAGATGG AAAGGAAGTG TCTATAAACA ATAACCGGGT CCACCTGAGT 
GCAGGGTTGC CCAATCCTAT CTCTGTCCCC ATCCTGTTTG AGGAGACCTT CTACAATGAG 
AAAGAGCAGG CCTACAGTAT ACTGTGCATC GCACGGCCCT TCGAATCTGA CCACATTCCT 
GAGGAGTTGG GTTCTGTTCC TGCTCCGTAC TGTCTGAAAA ATATTGAGGA TGTGAGGGAG 
TTTTTGGGCC GCCATGCTGA AAAACTGGAC AAGTTTGTTT CCTCCTTCTG TCTCTCCTTT 
AAAGAACAGG AGAGGAAAGG CCTACGCCAT CACATAGACT CGGTGAATAC GCTTTACACC 
AGATGTCTTC AGTGTCTGCT GAGAGACTCT CGTCTGAAGC TTTTGGCGAG ACAGGAGATT 
CAGATGACTC TACTCAAAGA AGCAGTAGAG ATGTACATCC ATCATGGCAT CCATGATCTA 
CTCTTTAGTT ATGTGGGCAC TCTTGAAGCT AGTCGGGATG CAGCGTTCAA CAAAACAACA 
CGGTCCTTGC AGGATCTGCA GCAGAAAGAG CTGGGAGTGA AGTCCGAGTT CAGTATTAAT 
ATTCCTCGGG CGAAGCGTGA GCTGATTCAG CTGAACGTCT GCACGTCTCC ACAGCAGAAA 
CTCCTCTGCA TCCGGAAAGT CATTTTAACC ATCATGCAGT CGACCAGACG CAGAGATACA 
GCGGTGAGCA TCGAGTCCAT GTGTGCCGAT GATCTGCTGT TGGTCATTCT GTATCTGCTC 
ATCAAGACAG AAATTCCCAA CTGGATGGCC AATCTGAGTT ATATAAAGCA CTTCCGCTTT 
CGGAGCTCCA GCAAGGATGA GTTGAGTTAC TGTCTGACGT CTTTCGAGGC CGCGGTCGAG 
TATATCAGCC TGGGAAACCT CAAACACAAC ACCGATGGGT CAGGAAACAG ACTGTCCTTC 
AGGCAGAAGG TGGACCTACT GTCCCAGAAT GCAACACCCA TTGACCGGCT GTTTGAGCAT 
ATTGTGAGCG GAAATGAGCA GGAGGTTCAG CGTTTGCTCA GTGAAAGCGA GAGTGAGGAG 
GACGGCAGGA AGTTGTGTCA CCCTCTGTGT TCCTGTGACG CCTGCGACCT GCGCATCTCT 
GGGAGATTGA ATGATCCTTC CATCGTCACT CCATTCTCTC GAGACGACAG AGGATACACT 
CCTTTACATG TTGCTGCTAT TTGTGGTCAG TCTCAGATGA TTGATTTACT GGTGTCAAAG 
GGAGCAGTGG TAAACGCTAC CGATTACCAT GCACTCACAC CGCTGCACCT CTCCTGCCAG 
AAGGGCTACC AGGGCGTCAC GCTTCTCCTG CTGCATTATA AGGCCAACAC TGATGCTCAG 
GACAACAATG GAAACACTCC TCTACATCTG GCCTGCATGT ACGGACATGA AGACTGTGTG 
AAGGCGCTGG TGTACTTCGA TCTGCACTGC TGTCGGCTGA ACGTTCAGAA CGATAAAGGA 
GACTCGCCGC TGCACATCGC CGCTCGCTGG GGATACGAGG GCATCATGGA GGTGCTGCTG 
GAGAACGGAG CGTCCACGCT CATCCACAAT AAAGCCAAGG AGACGCCGCT GCACTGCGCG 
CTCAACTCCA AGATTCTGTC ATTGTTGGAG CGCAGACACA ACGACTCCAG TATAAGGGAA 
AGTGGATTCG AGTCTCCGAG CCGCTCTCCT CCTCCGTCAG ACGGCAGCAG CAGACGCTCC 
TCCATCTCCA GCTCATCCTC TCTGAGTATT GAAACCTCTC CGCTGGAGTC TGACCGCAGC 
CGGCACAGAG AGGTGGAGAA GCTGTTGAGA GCCGTGGCTG ACGGAGATGT GGAGATGGTG 
CGTTATCTCC TGGAGTGGCT CGATGAGGAT CCAGATGAAG AGGAGTCTGC AGTGCCAGTC 
AAGACGGAGC TCTGCCATCC GCTGTGCCAG TGTGCCAACT GCGAACCCAC ACAGAAGAAG 
GCAGCTCATC TTCATCCAGA CAGTGTGGGT GTAAACAGCA GCAGTGTTGA CGGCTTCACG 
CCGCTGCATG TGGCGGCGCT GCATGGACAC ACACCTCTCA TCTCTCTGTT CACACGCCAC 
GGCGCAAACC TGAACGCTCG CAACAACCAC AGCGCCACCC CACTGCACCT GGCCTGCCAG 
AACAACCACA CAGAGGTGGT CTGTGCTCTC CTGGAGTGTA ATGCCAAACT CAATAAGAAG 
GATCATTATG GGAACACACC GCTCATTCAG GCCTGTCTGA AGGGGAATCT GGAGATCGCC 
GGCGTCCTGC TGGAGAGCGG TGCGCTGGTA AATCTGGCCA ATAACCACGG GAACACGGGT 
CTGCATGAGG CTGTGAGAGG AGCAAACGTG TCGCTGGTGG AGCTGCTGCT GCACAGAGGA 
GCTTCAGTTC ACATCCGCAA CAACAGACAG AGAACATCGC TGGACTGCGC ACACGAGGCC 
GCCGCCAAGA ACTCAGAGAT TTTGAGACTC CTTCAGAAAG CATATGATGA AGCCCAGACG 
CAAAGGGCCA GAGCACCAGA GGGCATCATG GCAAGAGTTA AACAGAGTCC TAACAATGGT 
GGACAGGATT TTGAGCACCA CAAACAGAGG AGTCTGAAGA GAACAGACAG AGCGGCAGCT 
ACAACAGCAG CAGCACAACA GACTGCAGGG AGCGACAGAC GGAGACTGAC CCGAGGAGAA 
ACACTGGACA TCAGTGATCC ACAGCCTCCG AGCAGACCTC TGACCCGCAG CCACACCATC 
AGCCAAGACC TGAGTTCCAG CGTTCAGCCC GAAGAGCAGA TCAACGGTGG AAAACGGGAG 
GATGAAGAAG ATCCGTCCGG TGCTGAAGCA GATCCTGCAG ACACTGAGAT GGACAGCGTG 
GTGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326421.1
CDS	50..3238
Translation	MAFLFLIRHLMDLKMKLYMVSKTLLNLHKYELEFGTHMAVYDENILKNPFYQALEKHRPDLCSRVAELHGIILVPCCGSLPTGSFTAAQFDGYVLQPSEQGYQTVDGKEVSINNNRVHLSAGLPNPISVPILFEETFYNEKEQAYSILCIARPFESDHIPEELGSVPAPYCLKNIEDVREFLGRHAEKLDKFVSSFCLSFKEQERKGLRHHIDSVNTLYTRCLQCLLRDSRLKLLARQEIQMTLLKEAVEMYIHHGIHDLLFSYVGTLEASRDAAFNKTTRSLQDLQQKELGVKSEFSINIPRAKRELIQLNVCTSPQQKLLCIRKVILTIMQSTRRRDTAVSIESMCADDLLLVILYLLIKTEIPNWMANLSYIKHFRFRSSSKDELSYCLTSFEAAVEYISLGNLKHNTDGSGNRLSFRQKVDLLSQNATPIDRLFEHIVSGNEQEVQRLLSESESEEDGRKLCHPLCSCDACDLRISGRLNDPSIVTPFSRDDRGYTPLHVAAICGQSQMIDLLVSKGAVVNATDYHALTPLHLSCQKGYQGVTLLLLHYKANTDAQDNNGNTPLHLACMYGHEDCVKALVYFDLHCCRLNVQNDKGDSPLHIAARWGYEGIMEVLLENGASTLIHNKAKETPLHCALNSKILSLLERRHNDSSIRESGFESPSRSPPPSDGSSRRSSISSSSSLSIETSPLESDRSRHREVEKLLRAVADGDVEMVRYLLEWLDEDPDEEESAVPVKTELCHPLCQCANCEPTQKKAAHLHPDSVGVNSSSVDGFTPLHVAALHGHTPLISLFTRHGANLNARNNHSATPLHLACQNNHTEVVCALLECNAKLNKKDHYGNTPLIQACLKGNLEIAGVLLESGALVNLANNHGNTGLHEAVRGANVSLVELLLHRGASVHIRNNRQRTSLDCAHEAAAKNSEILRLLQKAYDEAQTQRARAPEGIMARVKQSPNNGGQDFEHHKQRSLKRTDRAAATTAAAQQTAGSDRRRLTRGETLDISDPQPPSRPLTRSHTISQDLSSSVQPEEQINGGKREDEEDPSGAEADPADTEMDSVVL

Target ORF information:

RefSeq Version	XM_021470746.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470746.1

ORF Insert Sequence:

ATGGCGTTCC TCTTCCTCAT TCGTCATTTG ATGGATTTAA AGATGAAATT GTACATGGTG 
TCGAAAACTC TTCTTAACCT TCATAAATAT GAGCTGGAGT TTGGGACACA TATGGCTGTG 
TATGATGAGA ATATCCTGAA AAACCCTTTT TACCAGGCTC TGGAGAAACA CAGACCGGAC 
CTGTGCAGCC GGGTGGCAGA GCTCCATGGC ATTATCCTGG TACCGTGTTG TGGAAGTTTA 
CCAACGGGCT CTTTTACTGC AGCTCAGTTT GACGGATACG TTCTTCAACC TTCCGAGCAG 
GGTTACCAGA CTGTAGATGG AAAGGAAGTG TCTATAAACA ATAACCGGGT CCACCTGAGT 
GCAGGGTTGC CCAATCCTAT CTCTGTCCCC ATCCTGTTTG AGGAGACCTT CTACAATGAG 
AAAGAGCAGG CCTACAGTAT ACTGTGCATC GCACGGCCCT TCGAATCTGA CCACATTCCT 
GAGGAGTTGG GTTCTGTTCC TGCTCCGTAC TGTCTGAAAA ATATTGAGGA TGTGAGGGAG 
TTTTTGGGCC GCCATGCTGA AAAACTGGAC AAGTTTGTTT CCTCCTTCTG TCTCTCCTTT 
AAAGAACAGG AGAGGAAAGG CCTACGCCAT CACATAGACT CGGTGAATAC GCTTTACACC 
AGATGTCTTC AGTGTCTGCT GAGAGACTCT CGTCTGAAGC TTTTGGCGAG ACAGGAGATT 
CAGATGACTC TACTCAAAGA AGCAGTAGAG ATGTACATCC ATCATGGCAT CCATGATCTA 
CTCTTTAGTT ATGTGGGCAC TCTTGAAGCT AGTCGGGATG CAGCGTTCAA CAAAACAACA 
CGGTCCTTGC AGGATCTGCA GCAGAAAGAG CTGGGAGTGA AGTCCGAGTT CAGTATTAAT 
ATTCCTCGGG CGAAGCGTGA GCTGATTCAG CTGAACGTCT GCACGTCTCC ACAGCAGAAA 
CTCCTCTGCA TCCGGAAAGT CATTTTAACC ATCATGCAGT CGACCAGACG CAGAGATACA 
GCGGTGAGCA TCGAGTCCAT GTGTGCCGAT GATCTGCTGT TGGTCATTCT GTATCTGCTC 
ATCAAGACAG AAATTCCCAA CTGGATGGCC AATCTGAGTT ATATAAAGCA CTTCCGCTTT 
CGGAGCTCCA GCAAGGATGA GTTGAGTTAC TGTCTGACGT CTTTCGAGGC CGCGGTCGAG 
TATATCAGCC TGGGAAACCT CAAACACAAC ACCGATGGGT CAGGAAACAG ACTGTCCTTC 
AGGCAGAAGG TGGACCTACT GTCCCAGAAT GCAACACCCA TTGACCGGCT GTTTGAGCAT 
ATTGTGAGCG GAAATGAGCA GGAGGTTCAG CGTTTGCTCA GTGAAAGCGA GAGTGAGGAG 
GACGGCAGGA AGTTGTGTCA CCCTCTGTGT TCCTGTGACG CCTGCGACCT GCGCATCTCT 
GGGAGATTGA ATGATCCTTC CATCGTCACT CCATTCTCTC GAGACGACAG AGGATACACT 
CCTTTACATG TTGCTGCTAT TTGTGGTCAG TCTCAGATGA TTGATTTACT GGTGTCAAAG 
GGAGCAGTGG TAAACGCTAC CGATTACCAT GCACTCACAC CGCTGCACCT CTCCTGCCAG 
AAGGGCTACC AGGGCGTCAC GCTTCTCCTG CTGCATTATA AGGCCAACAC TGATGCTCAG 
GACAACAATG GAAACACTCC TCTACATCTG GCCTGCATGT ACGGACATGA AGACTGTGTG 
AAGGCGCTGG TGTACTTCGA TCTGCACTGC TGTCGGCTGA ACGTTCAGAA CGATAAAGGA 
GACTCGCCGC TGCACATCGC CGCTCGCTGG GGATACGAGG GCATCATGGA GGTGCTGCTG 
GAGAACGGAG CGTCCACGCT CATCCACAAT AAAGCCAAGG AGACGCCGCT GCACTGCGCG 
CTCAACTCCA AGATTCTGTC ATTGTTGGAG CGCAGACACA ACGACTCCAG TATAAGGGAA 
AGTGGATTCG AGTCTCCGAG CCGCTCTCCT CCTCCGTCAG ACGGCAGCAG CAGACGCTCC 
TCCATCTCCA GCTCATCCTC TCTGAGTATT GAAACCTCTC CGCTGGAGTC TGACCGCAGC 
CGGCACAGAG AGGTGGAGAA GCTGTTGAGA GCCGTGGCTG ACGGAGATGT GGAGATGGTG 
CGTTATCTCC TGGAGTGGCT CGATGAGGAT CCAGATGAAG AGGAGTCTGC AGTGCCAGTC 
AAGACGGAGC TCTGCCATCC GCTGTGCCAG TGTGCCAACT GCGAACCCAC ACAGAAGAAG 
GCAGCTCATC TTCATCCAGA CAGTGTGGGT GTAAACAGCA GCAGTGTTGA CGGCTTCACG 
CCGCTGCATG TGGCGGCGCT GCATGGACAC ACACCTCTCA TCTCTCTGTT CACACGCCAC 
GGCGCAAACC TGAACGCTCG CAACAACCAC AGCGCCACCC CACTGCACCT GGCCTGCCAG 
AACAACCACA CAGAGGTGGT CTGTGCTCTC CTGGAGTGTA ATGCCAAACT CAATAAGAAG 
GATCATTATG GGAACACACC GCTCATTCAG GCCTGTCTGA AGGGGAATCT GGAGATCGCC 
GGCGTCCTGC TGGAGAGCGG TGCGCTGGTA AATCTGGCCA ATAACCACGG GAACACGGGT 
CTGCATGAGG CTGTGAGAGG AGCAAACGTG TCGCTGGTGG AGCTGCTGCT GCACAGAGGA 
GCTTCAGTTC ACATCCGCAA CAACAGACAG AGAACATCGC TGGACTGCGC ACACGAGGCC 
GCCGCCAAGA ACTCAGAGAT TTTGAGACTC CTTCAGAAAG CATATGATGA AGCCCAGACG 
CAAAGGGCCA GAGCACCAGA GGGCATCATG GCAAGAGTTA AACAGAGTCC TAACAATGGT 
GGACAGGATT TTGAGCACCA CAAACAGAGG AGTCTGAAGA GAACAGACAG AGCGGCAGCT 
ACAACAGCAG CAGCACAACA GACTGCAGGG AGCGACAGAC GGAGACTGAC CCGAGGAGAA 
ACACTGGACA TCAGTGATCC ACAGCCTCCG AGCAGACCTC TGACCCGCAG CCACACCATC 
AGCCAAGACC TGAGTTCCAG CGTTCAGCCC GAAGAGCAGA TCAACGGTGG AAAACGGGAG 
GATGAAGAAG ATCCGTCCGG TGCTGAAGCA GATCCTGCAG ACACTGAGAT GGACAGCGTG 
GTGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD27 ( XM_021470748.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326423.1 Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa141497
Clone ID Related Accession (Same CDS sequence)	XM_021470748.1 , XM_021470747.1
Accession Version	XM_021470748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat domain-containing protein 27 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 983 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT 
GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT 
TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC 
CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC 
TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC 
CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG 
GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC 
TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT 
ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG 
AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC 
ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC 
AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG 
TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT 
CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA 
CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT 
CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG 
CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG 
GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC 
AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG 
CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC 
GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC 
CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC 
AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA 
CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC 
CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC 
ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT 
GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG 
AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG 
GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG 
CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC 
AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC 
AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG 
TCTGACCGCA GCCGGCACAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT 
GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTCGATGAGG ATCCAGATGA AGAGGAGTCT 
GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC 
ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTAAACAG CAGCAGTGTT 
GACGGCTTCA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG 
TTCACACGCC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC 
CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA 
CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT 
CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC 
GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGTCGCTGGT GGAGCTGCTG 
CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACATC GCTGGACTGC 
GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT 
GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT 
CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC 
AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG 
ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC 
AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT 
GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG 
ATGGACAGCG TGGTGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326423.1
CDS	41..3121
Translation	MAVYDENILKNPFYQALEKHRPDLCSRVAELHGIILVPCCGSLPTGSFTAAQFDGYVLQPSEQGYQTVDGKEVSINNNRVHLSAGLPNPISVPILFEETFYNEKEQAYSILCIARPFESDHIPAEELGSVPAPYCLKNIEDVREFLGRHAEKLDKFVSSFCLSFKEQERKGLRHHIDSVNTLYTRCLQCLLRDSRLKLLARQEIQMTLLKEAVEMYIHHGIHDLLFSYVGTLEASRDAAFNKTTRSLQDLQQKELGVKSEFSINIPRAKRELIQLNVCTSPQQKLLCIRKVILTIMQSTRRRDTAVSIESMCADDLLLVILYLLIKTEIPNWMANLSYIKHFRFRSSSKDELSYCLTSFEAAVEYISLGNLKHNTDGSGNRLSFRQKVDLLSQNATPIDRLFEHIVSGNEQEVQRLLSESESEEDGRKLCHPLCSCDACDLRISGRLNDPSIVTPFSRDDRGYTPLHVAAICGQSQMIDLLVSKGAVVNATDYHALTPLHLSCQKGYQGVTLLLLHYKANTDAQDNNGNTPLHLACMYGHEDCVKALVYFDLHCCRLNVQNDKGDSPLHIAARWGYEGIMEVLLENGASTLIHNKAKETPLHCALNSKILSLLERRHNDSSIRESGFESPSRSPPPSDGSSRRSSISSSSSLSIETSPLESDRSRHREVEKLLRAVADGDVEMVRYLLEWLDEDPDEEESAVPVKTELCHPLCQCANCEPTQKKAAHLHPDSVGVNSSSVDGFTPLHVAALHGHTPLISLFTRHGANLNARNNHSATPLHLACQNNHTEVVCALLECNAKLNKKDHYGNTPLIQACLKGNLEIAGVLLESGALVNLANNHGNTGLHEAVRGANVSLVELLLHRGASVHIRNNRQRTSLDCAHEAAAKNSEILRLLQKAYDEAQTQRARAPEGIMARVKQSPNNGGQDFEHHKQRSLKRTDRAAATTAAAQQTAGSDRRRLTRGETLDISDPQPPSRPLTRSHTISQDLSSSVQPEEQINGGKREDEEDPSGAEADPADTEMDSVVL

Target ORF information:

RefSeq Version	XM_021470748.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470748.1

ORF Insert Sequence:

ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT 
GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT 
TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC 
CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC 
TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC 
CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG 
GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC 
TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT 
ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG 
AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC 
ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC 
AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG 
TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT 
CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA 
CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT 
CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG 
CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG 
GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC 
AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG 
CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC 
GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC 
CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC 
AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA 
CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC 
CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC 
ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT 
GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG 
AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG 
GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG 
CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC 
AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC 
AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG 
TCTGACCGCA GCCGGCACAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT 
GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTCGATGAGG ATCCAGATGA AGAGGAGTCT 
GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC 
ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTAAACAG CAGCAGTGTT 
GACGGCTTCA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG 
TTCACACGCC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC 
CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA 
CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT 
CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC 
GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGTCGCTGGT GGAGCTGCTG 
CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACATC GCTGGACTGC 
GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT 
GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT 
CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC 
AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG 
ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC 
AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT 
GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG 
ATGGACAGCG TGGTGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD27 ( XM_021470747.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326422.1 Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa141497
Clone ID Related Accession (Same CDS sequence)	XM_021470748.1 , XM_021470747.1
Accession Version	XM_021470747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat domain-containing protein 27 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 983 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT 
GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT 
TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC 
CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC 
TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC 
CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG 
GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC 
TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT 
ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG 
AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC 
ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC 
AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG 
TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT 
CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA 
CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT 
CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG 
CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG 
GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC 
AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG 
CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC 
GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC 
CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC 
AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA 
CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC 
CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC 
ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT 
GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG 
AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG 
GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG 
CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC 
AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC 
AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG 
TCTGACCGCA GCCGGCACAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT 
GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTCGATGAGG ATCCAGATGA AGAGGAGTCT 
GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC 
ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTAAACAG CAGCAGTGTT 
GACGGCTTCA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG 
TTCACACGCC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC 
CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA 
CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT 
CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC 
GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGTCGCTGGT GGAGCTGCTG 
CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACATC GCTGGACTGC 
GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT 
GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT 
CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC 
AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG 
ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC 
AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT 
GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG 
ATGGACAGCG TGGTGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326422.1
CDS	171..3251
Translation	MAVYDENILKNPFYQALEKHRPDLCSRVAELHGIILVPCCGSLPTGSFTAAQFDGYVLQPSEQGYQTVDGKEVSINNNRVHLSAGLPNPISVPILFEETFYNEKEQAYSILCIARPFESDHIPAEELGSVPAPYCLKNIEDVREFLGRHAEKLDKFVSSFCLSFKEQERKGLRHHIDSVNTLYTRCLQCLLRDSRLKLLARQEIQMTLLKEAVEMYIHHGIHDLLFSYVGTLEASRDAAFNKTTRSLQDLQQKELGVKSEFSINIPRAKRELIQLNVCTSPQQKLLCIRKVILTIMQSTRRRDTAVSIESMCADDLLLVILYLLIKTEIPNWMANLSYIKHFRFRSSSKDELSYCLTSFEAAVEYISLGNLKHNTDGSGNRLSFRQKVDLLSQNATPIDRLFEHIVSGNEQEVQRLLSESESEEDGRKLCHPLCSCDACDLRISGRLNDPSIVTPFSRDDRGYTPLHVAAICGQSQMIDLLVSKGAVVNATDYHALTPLHLSCQKGYQGVTLLLLHYKANTDAQDNNGNTPLHLACMYGHEDCVKALVYFDLHCCRLNVQNDKGDSPLHIAARWGYEGIMEVLLENGASTLIHNKAKETPLHCALNSKILSLLERRHNDSSIRESGFESPSRSPPPSDGSSRRSSISSSSSLSIETSPLESDRSRHREVEKLLRAVADGDVEMVRYLLEWLDEDPDEEESAVPVKTELCHPLCQCANCEPTQKKAAHLHPDSVGVNSSSVDGFTPLHVAALHGHTPLISLFTRHGANLNARNNHSATPLHLACQNNHTEVVCALLECNAKLNKKDHYGNTPLIQACLKGNLEIAGVLLESGALVNLANNHGNTGLHEAVRGANVSLVELLLHRGASVHIRNNRQRTSLDCAHEAAAKNSEILRLLQKAYDEAQTQRARAPEGIMARVKQSPNNGGQDFEHHKQRSLKRTDRAAATTAAAQQTAGSDRRRLTRGETLDISDPQPPSRPLTRSHTISQDLSSSVQPEEQINGGKREDEEDPSGAEADPADTEMDSVVL

Target ORF information:

RefSeq Version	XM_021470747.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470747.1

ORF Insert Sequence:

ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT 
GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT 
TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC 
CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC 
TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC 
CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG 
GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC 
TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT 
ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG 
AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC 
ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC 
AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG 
TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT 
CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA 
CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT 
CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG 
CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG 
GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC 
AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG 
CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC 
GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC 
CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC 
AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA 
CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC 
CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC 
ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT 
GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG 
AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG 
GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG 
CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC 
AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC 
AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG 
TCTGACCGCA GCCGGCACAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT 
GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTCGATGAGG ATCCAGATGA AGAGGAGTCT 
GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC 
ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTAAACAG CAGCAGTGTT 
GACGGCTTCA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG 
TTCACACGCC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC 
CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA 
CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT 
CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC 
GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGTCGCTGGT GGAGCTGCTG 
CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACATC GCTGGACTGC 
GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT 
GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT 
CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC 
AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG 
ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC 
AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT 
GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG 
ATGGACAGCG TGGTGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD27 ( XM_021470749.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326424.1 Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa141498
Clone ID Related Accession (Same CDS sequence)	XM_021470749.1
Accession Version	XM_021470749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat domain-containing protein 27 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 983 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT 
GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT 
TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC 
CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC 
TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC 
CACATTCCTG AGGAGTTGGG TTCTGTTCCT GCTCCGTACT GTCTGAAAAA TATTGAGGAT 
GTGAGGGAGT TTTTGGGCCG CCATGCTGAA AAACTGGACA AGTTTGTTTC CTCCTTCTGT 
CTCTCCTTTA AAGAACAGGA GAGGAAAGGC CTACGCCATC ACATAGACTC GGTGAATACG 
CTTTACACCA GATGTCTTCA GTGTCTGCTG AGAGACTCTC GTCTGAAGCT TTTGGCGAGA 
CAGGAGATTC AGATGACTCT ACTCAAAGAA GCAGTAGAGA TGTACATCCA TCATGGCATC 
CATGATCTAC TCTTTAGTTA TGTGGGCACT CTTGAAGCTA GTCGGGATGC AGCGTTCAAC 
AAAACAACAC GGTCCTTGCA GGATCTGCAG CAGAAAGAGC TGGGAGTGAA GTCCGAGTTC 
AGTATTAATA TTCCTCGGGC GAAGCGTGAG CTGATTCAGC TGAACGTCTG CACGTCTCCA 
CAGCAGAAAC TCCTCTGCAT CCGGAAAGTC ATTTTAACCA TCATGCAGTC GACCAGACGC 
AGAGATACAG CGGTGAGCAT CGAGTCCATG TGTGCCGATG ATCTGCTGTT GGTCATTCTG 
TATCTGCTCA TCAAGACAGA AATTCCCAAC TGGATGGCCA ATCTGAGTTA TATAAAGCAC 
TTCCGCTTTC GGAGCTCCAG CAAGGATGAG TTGAGTTACT GTCTGACGTC TTTCGAGGCC 
GCGGTCGAGT ATATCAGCCT GGGAAACCTC AAACACAACA CCGATGGGTC AGGAAACAGA 
CTGTCCTTCA GGCAGAAGGT GGACCTACTG TCCCAGAATG CAACACCCAT TGACCGGCTG 
TTTGAGCATA TTGTGAGCGG AAATGAGCAG GAGGTTCAGC GTTTGCTCAG TGAAAGCGAG 
AGTGAGGAGG ACGGCAGGAA GTTGTGTCAC CCTCTGTGTT CCTGTGACGC CTGCGACCTG 
CGCATCTCTG GGAGATTGAA TGATCCTTCC ATCGTCACTC CATTCTCTCG AGACGACAGA 
GGATACACTC CTTTACATGT TGCTGCTATT TGTGGTCAGT CTCAGATGAT TGATTTACTG 
GTGTCAAAGG GAGCAGTGGT AAACGCTACC GATTACCATG CACTCACACC GCTGCACCTC 
TCCTGCCAGA AGGGCTACCA GGGCGTCACG CTTCTCCTGC TGCATTATAA GGCCAACACT 
GATGCTCAGG ACAACAATGG AAACACTCCT CTACATCTGG CCTGCATGTA CGGACATGAA 
GACTGTGTGA AGGCGCTGGT GTACTTCGAT CTGCACTGCT GTCGGCTGAA CGTTCAGAAC 
GATAAAGGAG ACTCGCCGCT GCACATCGCC GCTCGCTGGG GATACGAGGG CATCATGGAG 
GTGCTGCTGG AGAACGGAGC GTCCACGCTC ATCCACAATA AAGCCAAGGA GACGCCGCTG 
CACTGCGCGC TCAACTCCAA GATTCTGTCA TTGTTGGAGC GCAGACACAA CGACTCCAGT 
ATAAGGGAAA GTGGATTCGA GTCTCCGAGC CGCTCTCCTC CTCCGTCAGA CGGCAGCAGC 
AGACGCTCCT CCATCTCCAG CTCATCCTCT CTGAGTATTG AAACCTCTCC GCTGGAGTCT 
GACCGCAGCC GGCACAGAGA GGTGGAGAAG CTGTTGAGAG CCGTGGCTGA CGGAGATGTG 
GAGATGGTGC GTTATCTCCT GGAGTGGCTC GATGAGGATC CAGATGAAGA GGAGTCTGCA 
GTGCCAGTCA AGACGGAGCT CTGCCATCCG CTGTGCCAGT GTGCCAACTG CGAACCCACA 
CAGAAGAAGG CAGCTCATCT TCATCCAGAC AGTGTGGGTG TAAACAGCAG CAGTGTTGAC 
GGCTTCACGC CGCTGCATGT GGCGGCGCTG CATGGACACA CACCTCTCAT CTCTCTGTTC 
ACACGCCACG GCGCAAACCT GAACGCTCGC AACAACCACA GCGCCACCCC ACTGCACCTG 
GCCTGCCAGA ACAACCACAC AGAGGTGGTC TGTGCTCTCC TGGAGTGTAA TGCCAAACTC 
AATAAGAAGG ATCATTATGG GAACACACCG CTCATTCAGG CCTGTCTGAA GGGGAATCTG 
GAGATCGCCG GCGTCCTGCT GGAGAGCGGT GCGCTGGTAA ATCTGGCCAA TAACCACGGG 
AACACGGGTC TGCATGAGGC TGTGAGAGGA GCAAACGTGT CGCTGGTGGA GCTGCTGCTG 
CACAGAGGAG CTTCAGTTCA CATCCGCAAC AACAGACAGA GAACATCGCT GGACTGCGCA 
CACGAGGCCG CCGCCAAGAA CTCAGAGATT TTGAGACTCC TTCAGAAAGC ATATGATGAA 
GCCCAGACGC AAAGGGCCAG AGCACCAGAG GGCATCATGG CAAGAGTTAA ACAGAGTCCT 
AACAATGGTG GACAGGATTT TGAGCACCAC AAACAGAGGA GTCTGAAGAG AACAGACAGA 
GCGGCAGCTA CAACAGCAGC AGCACAACAG ACTGCAGGGA GCGACAGACG GAGACTGACC 
CGAGGAGAAA CACTGGACAT CAGTGATCCA CAGCCTCCGA GCAGACCTCT GACCCGCAGC 
CACACCATCA GCCAAGACCT GAGTTCCAGC GTTCAGCCCG AAGAGCAGAT CAACGGTGGA 
AAACGGGAGG ATGAAGAAGA TCCGTCCGGT GCTGAAGCAG ATCCTGCAGA CACTGAGATG 
GACAGCGTGG TGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326424.1
CDS	171..3248
Translation	MAVYDENILKNPFYQALEKHRPDLCSRVAELHGIILVPCCGSLPTGSFTAAQFDGYVLQPSEQGYQTVDGKEVSINNNRVHLSAGLPNPISVPILFEETFYNEKEQAYSILCIARPFESDHIPEELGSVPAPYCLKNIEDVREFLGRHAEKLDKFVSSFCLSFKEQERKGLRHHIDSVNTLYTRCLQCLLRDSRLKLLARQEIQMTLLKEAVEMYIHHGIHDLLFSYVGTLEASRDAAFNKTTRSLQDLQQKELGVKSEFSINIPRAKRELIQLNVCTSPQQKLLCIRKVILTIMQSTRRRDTAVSIESMCADDLLLVILYLLIKTEIPNWMANLSYIKHFRFRSSSKDELSYCLTSFEAAVEYISLGNLKHNTDGSGNRLSFRQKVDLLSQNATPIDRLFEHIVSGNEQEVQRLLSESESEEDGRKLCHPLCSCDACDLRISGRLNDPSIVTPFSRDDRGYTPLHVAAICGQSQMIDLLVSKGAVVNATDYHALTPLHLSCQKGYQGVTLLLLHYKANTDAQDNNGNTPLHLACMYGHEDCVKALVYFDLHCCRLNVQNDKGDSPLHIAARWGYEGIMEVLLENGASTLIHNKAKETPLHCALNSKILSLLERRHNDSSIRESGFESPSRSPPPSDGSSRRSSISSSSSLSIETSPLESDRSRHREVEKLLRAVADGDVEMVRYLLEWLDEDPDEEESAVPVKTELCHPLCQCANCEPTQKKAAHLHPDSVGVNSSSVDGFTPLHVAALHGHTPLISLFTRHGANLNARNNHSATPLHLACQNNHTEVVCALLECNAKLNKKDHYGNTPLIQACLKGNLEIAGVLLESGALVNLANNHGNTGLHEAVRGANVSLVELLLHRGASVHIRNNRQRTSLDCAHEAAAKNSEILRLLQKAYDEAQTQRARAPEGIMARVKQSPNNGGQDFEHHKQRSLKRTDRAAATTAAAQQTAGSDRRRLTRGETLDISDPQPPSRPLTRSHTISQDLSSSVQPEEQINGGKREDEEDPSGAEADPADTEMDSVVL

Target ORF information:

RefSeq Version	XM_021470749.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470749.1

ORF Insert Sequence:

ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT 
GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT 
TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC 
CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC 
TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC 
CACATTCCTG AGGAGTTGGG TTCTGTTCCT GCTCCGTACT GTCTGAAAAA TATTGAGGAT 
GTGAGGGAGT TTTTGGGCCG CCATGCTGAA AAACTGGACA AGTTTGTTTC CTCCTTCTGT 
CTCTCCTTTA AAGAACAGGA GAGGAAAGGC CTACGCCATC ACATAGACTC GGTGAATACG 
CTTTACACCA GATGTCTTCA GTGTCTGCTG AGAGACTCTC GTCTGAAGCT TTTGGCGAGA 
CAGGAGATTC AGATGACTCT ACTCAAAGAA GCAGTAGAGA TGTACATCCA TCATGGCATC 
CATGATCTAC TCTTTAGTTA TGTGGGCACT CTTGAAGCTA GTCGGGATGC AGCGTTCAAC 
AAAACAACAC GGTCCTTGCA GGATCTGCAG CAGAAAGAGC TGGGAGTGAA GTCCGAGTTC 
AGTATTAATA TTCCTCGGGC GAAGCGTGAG CTGATTCAGC TGAACGTCTG CACGTCTCCA 
CAGCAGAAAC TCCTCTGCAT CCGGAAAGTC ATTTTAACCA TCATGCAGTC GACCAGACGC 
AGAGATACAG CGGTGAGCAT CGAGTCCATG TGTGCCGATG ATCTGCTGTT GGTCATTCTG 
TATCTGCTCA TCAAGACAGA AATTCCCAAC TGGATGGCCA ATCTGAGTTA TATAAAGCAC 
TTCCGCTTTC GGAGCTCCAG CAAGGATGAG TTGAGTTACT GTCTGACGTC TTTCGAGGCC 
GCGGTCGAGT ATATCAGCCT GGGAAACCTC AAACACAACA CCGATGGGTC AGGAAACAGA 
CTGTCCTTCA GGCAGAAGGT GGACCTACTG TCCCAGAATG CAACACCCAT TGACCGGCTG 
TTTGAGCATA TTGTGAGCGG AAATGAGCAG GAGGTTCAGC GTTTGCTCAG TGAAAGCGAG 
AGTGAGGAGG ACGGCAGGAA GTTGTGTCAC CCTCTGTGTT CCTGTGACGC CTGCGACCTG 
CGCATCTCTG GGAGATTGAA TGATCCTTCC ATCGTCACTC CATTCTCTCG AGACGACAGA 
GGATACACTC CTTTACATGT TGCTGCTATT TGTGGTCAGT CTCAGATGAT TGATTTACTG 
GTGTCAAAGG GAGCAGTGGT AAACGCTACC GATTACCATG CACTCACACC GCTGCACCTC 
TCCTGCCAGA AGGGCTACCA GGGCGTCACG CTTCTCCTGC TGCATTATAA GGCCAACACT 
GATGCTCAGG ACAACAATGG AAACACTCCT CTACATCTGG CCTGCATGTA CGGACATGAA 
GACTGTGTGA AGGCGCTGGT GTACTTCGAT CTGCACTGCT GTCGGCTGAA CGTTCAGAAC 
GATAAAGGAG ACTCGCCGCT GCACATCGCC GCTCGCTGGG GATACGAGGG CATCATGGAG 
GTGCTGCTGG AGAACGGAGC GTCCACGCTC ATCCACAATA AAGCCAAGGA GACGCCGCTG 
CACTGCGCGC TCAACTCCAA GATTCTGTCA TTGTTGGAGC GCAGACACAA CGACTCCAGT 
ATAAGGGAAA GTGGATTCGA GTCTCCGAGC CGCTCTCCTC CTCCGTCAGA CGGCAGCAGC 
AGACGCTCCT CCATCTCCAG CTCATCCTCT CTGAGTATTG AAACCTCTCC GCTGGAGTCT 
GACCGCAGCC GGCACAGAGA GGTGGAGAAG CTGTTGAGAG CCGTGGCTGA CGGAGATGTG 
GAGATGGTGC GTTATCTCCT GGAGTGGCTC GATGAGGATC CAGATGAAGA GGAGTCTGCA 
GTGCCAGTCA AGACGGAGCT CTGCCATCCG CTGTGCCAGT GTGCCAACTG CGAACCCACA 
CAGAAGAAGG CAGCTCATCT TCATCCAGAC AGTGTGGGTG TAAACAGCAG CAGTGTTGAC 
GGCTTCACGC CGCTGCATGT GGCGGCGCTG CATGGACACA CACCTCTCAT CTCTCTGTTC 
ACACGCCACG GCGCAAACCT GAACGCTCGC AACAACCACA GCGCCACCCC ACTGCACCTG 
GCCTGCCAGA ACAACCACAC AGAGGTGGTC TGTGCTCTCC TGGAGTGTAA TGCCAAACTC 
AATAAGAAGG ATCATTATGG GAACACACCG CTCATTCAGG CCTGTCTGAA GGGGAATCTG 
GAGATCGCCG GCGTCCTGCT GGAGAGCGGT GCGCTGGTAA ATCTGGCCAA TAACCACGGG 
AACACGGGTC TGCATGAGGC TGTGAGAGGA GCAAACGTGT CGCTGGTGGA GCTGCTGCTG 
CACAGAGGAG CTTCAGTTCA CATCCGCAAC AACAGACAGA GAACATCGCT GGACTGCGCA 
CACGAGGCCG CCGCCAAGAA CTCAGAGATT TTGAGACTCC TTCAGAAAGC ATATGATGAA 
GCCCAGACGC AAAGGGCCAG AGCACCAGAG GGCATCATGG CAAGAGTTAA ACAGAGTCCT 
AACAATGGTG GACAGGATTT TGAGCACCAC AAACAGAGGA GTCTGAAGAG AACAGACAGA 
GCGGCAGCTA CAACAGCAGC AGCACAACAG ACTGCAGGGA GCGACAGACG GAGACTGACC 
CGAGGAGAAA CACTGGACAT CAGTGATCCA CAGCCTCCGA GCAGACCTCT GACCCGCAGC 
CACACCATCA GCCAAGACCT GAGTTCCAGC GTTCAGCCCG AAGAGCAGAT CAACGGTGGA 
AAACGGGAGG ATGAAGAAGA TCCGTCCGGT GCTGAAGCAG ATCCTGCAGA CACTGAGATG 
GACAGCGTGG TGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD27 ( XM_017354281.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209770.1 Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa57976
Clone ID Related Accession (Same CDS sequence)	XM_017354281.1
Accession Version	XM_017354281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin repeat domain-containing protein 27 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 2012 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCGTTCC TCTTCCTCAT TCGTCATTTG ATGGATTTAA AGATGAAATT GTACATGGTG 
TCGAAAACTC TTCTTAACCT TCATAAATAT GAGCTGGAGT TTGGGACACA TATGGCTGTG 
TATGATGAGA ATATCCTGAA AAACCCTTTT TACCAGGCTC TGGAGAAACA CAGACCGGAC 
CTGTGCAGCC GGGTGGCAGA GCTCCATGGC ATTATCCTGG TACCGTGTTG TGGAAGTTTA 
CCAACGGGCT CTTTTACTGC AGCTCAGTTT GACGGATACG TTCTTCAACC TTCCGAGCAG 
GGTTACCAGA CTGTAGATGG AAAGGAAGTG TCTATAAACA ATAACCGGGT CCACCTGAGT 
GCAGGGTTGC CCAATCCTAT CTCTGTCCCC ATCCTGTTTG AGGAGACCTT CTACAATGAG 
AAAGAGCAGG CCTACAGTAT ACTGTGCATC GCACGGCCCT TCGAATCTGA CCACATTCCT 
GCTGAGGAGT TGGGTTCTGT TCCTGCTCCG TACTGTCTGA AAAATATTGA GGATGTGAGG 
GAGTTTTTGG GCCGCCATGC TGAAAAACTG GACAAGTTTG TTTCCTCCTT CTGTCTCTCC 
TTTAAAGAAC AGGAGAGGAA AGGCCTACGC CATCACATAG ACTCGGTGAA TACGCTTTAC 
ACCAGATGTC TTCAGTGTCT GCTGAGAGAC TCTCGTCTGA AGCTTTTGGC GAGACAGGAG 
ATTCAGATGA CTCTACTCAA AGAAGCAGTA GAGATGTACA TCCATCATGG CATCCATGAT 
CTACTCTTTA GTTATGTGGG CACTCTTGAA GCTAGTCGGG ATGCAGCGTT CAACAAAACA 
ACACGGTCCT TGCAGGATCT GCAGCAGAAA GAGCTGGGAG TGAAGTCCGA GTTCAGTATT 
AATATTCCTC GGGCGAAGCG TGAGCTGATT CAGCTGAACG TCTGCACGTC TCCACAGCAG 
AAACTCCTCT GCATCCGGAA AGTCATTTTA ACCATCATGC AGTCGACCAG ACGCAGAGAT 
ACAGCGGTGA GCATCGAGTC CATGTGTGCC GATGATCTGC TGTTGGTCAT TCTGTATCTG 
CTCATCAAGA CAGAAATTCC CAACTGGATG GCCAATCTGA GTTATATAAA GCACTTCCGC 
TTTCGGAGCT CCAGCAAGGA TGAGTTGAGT TACTGTCTGA CGTCTTTCGA GGCCGCGGTC 
GAGTATATCA GCCTGGGAAA CCTCAAACAC AACACCGATG GGTCAGGAAA CAGACTGTCC 
TTCAGGCAGA AGGTGGACCT ACTGTCCCAG AATGCAACAC CCATTGACCG GCTGTTTGAG 
CATATTGTGA GCGGAAATGA GCAGGAGGTT CAGCGTTTGC TCAGTGAAAG CGAGAGTGAG 
GAGGACGGCA GGAAGTTGTG TCACCCTCTG TGTTCCTGTG ACGCCTGCGA CCTGCGCATC 
TCTGGGAGAT TGAATGATCC TTCCATCGTC ACTCCATTCT CTCGAGACGA CAGAGGATAC 
ACTCCTTTAC ATGTTGCTGC TATTTGTGGT CAGTCTCAGA TGATTGATTT ACTGGTGTCA 
AAGGGAGCAG TGGTAAACGC TACCGATTAC CATGCACTCA CACCGCTGCA CCTCTCCTGC 
CAGAAGGGCT ACCAGGGCGT CACGCTTCTC CTGCTGCATT ATAAGGCCAA CACTGATGCT 
CAGGACAACA ATGGAAACAC TCCTCTACAT CTGGCCTGCA TGTACGGACA TGAAGACTGT 
GTGAAGGCGC TGGTGTACTT CGATCTGCAC TGCTGTCGGC TGAACGTTCA GAACGATAAA 
GGAGACTCGC CGCTGCACAT CGCCGCTCGC TGGGGATACG AGGGCATCAT GGAGGTGCTG 
CTGGAGAACG GAGCGTCCAC GCTCATCCAC AATAAAGCCA AGGAGACGCC GCTGCACTGC 
GCGCTCAACT CCAAGATTCT GTCATTGTTG GAGCGCAGAC ACAACGACTC CAGTATAAGG 
GAAAGTGGAT TCGAGTCTCC GAGCCGCTCT CCTCCTCCGT CAGACGGCAG CAGCAGACGC 
TCCTCCATCT CCAGCTCATC CTCTCTGAGT ATTGAAACCT CTCCGCTGGA GTCTGACCGC 
AGCCGGCATA GAGAGGTGGA GAAGCTGTTG AGAGCCGTGG CTGACGGAGA TGTGGAGATG 
GTGCGTTATC TCCTGGAGTG GCTGGATGAG GATCCAGATG AAGAGGAGTC TGCAGTGCCA 
GTCAAGACGG AGCTCTGCCA TCCGCTGTGC CAGTGTGCCA ACTGCGAACC CACACAGAAG 
AAGGCAGCTC ATCTTCATCC AGACAGTGTG GGTGTGAACA GCAGCAGTGT TGACGGCTTT 
ACGCCGCTGC ATGTGGCGGC GCTGCATGGA CACACACCTC TCATCTCTCT GTTCACACAC 
CACGGCGCAA ACCTGAACGC TCGCAACAAC CACAGCGCCA CCCCACTGCA CCTGGCCTGC 
CAGAACAACC ACACAGAGGT GGTCTGTGCT CTCCTGGAGT GTAATGCCAA ACTCAATAAG 
AAGGATCATT ATGGGAACAC ACCGCTCATT CAGGCCTGTC TGAAGGGGAA TCTGGAGATC 
GCCGGCGTCC TGCTGGAGAG CGGTGCGCTG GTAAATCTGG CCAATAACCA CGGGAACACG 
GGTCTGCATG AGGCTGTGAG AGGAGCAAAC GTGCCGCTGG TGGAGCTGCT GCTGCACAGA 
GGAGCTTCAG TTCACATCCG CAACAACAGA CAGAGAACAG CGCTGGACTG CGCACACGAG 
GCCGCCGCCA AGAACTCAGA GATTTTGAGA CTCCTTCAGA AAGCATATGA TGAAGCCCAG 
ACGCAAAGGG CCAGAGCACC AGAGGGCATC ATGGCAAGAG TTAAACAGAG TCCTAACAAT 
GGTGGACAGG ATTTTGAGCA CCACAAACAG AGGAGTCTGA AGAGAACAGA CAGAGCGGCA 
GCTACAACAG CAGCAGCACA ACAGACTGCA GGGAGCGACA GACGGAGACT GACCCGAGGA 
GAAACACTGG ACATCAGTGA TCCACAGCCT CCGAGCAGAC CTCTGACCCG CAGCCACACC 
ATCAGCCAAG ACCTGAGTTC CAGCGTTCAG CCCGAAGAGC AGATCAACGG TGGAAAACGG 
GAGGATGAAG AAGATCCGTC CGGTGCTGAA GCAGATCCTG CAGACACTGA GATGGACAGC 
GTGGTGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209770.1
CDS	185..3376
Translation	MAFLFLIRHLMDLKMKLYMVSKTLLNLHKYELEFGTHMAVYDENILKNPFYQALEKHRPDLCSRVAELHGIILVPCCGSLPTGSFTAAQFDGYVLQPSEQGYQTVDGKEVSINNNRVHLSAGLPNPISVPILFEETFYNEKEQAYSILCIARPFESDHIPAEELGSVPAPYCLKNIEDVREFLGRHAEKLDKFVSSFCLSFKEQERKGLRHHIDSVNTLYTRCLQCLLRDSRLKLLARQEIQMTLLKEAVEMYIHHGIHDLLFSYVGTLEASRDAAFNKTTRSLQDLQQKELGVKSEFSINIPRAKRELIQLNVCTSPQQKLLCIRKVILTIMQSTRRRDTAVSIESMCADDLLLVILYLLIKTEIPNWMANLSYIKHFRFRSSSKDELSYCLTSFEAAVEYISLGNLKHNTDGSGNRLSFRQKVDLLSQNATPIDRLFEHIVSGNEQEVQRLLSESESEEDGRKLCHPLCSCDACDLRISGRLNDPSIVTPFSRDDRGYTPLHVAAICGQSQMIDLLVSKGAVVNATDYHALTPLHLSCQKGYQGVTLLLLHYKANTDAQDNNGNTPLHLACMYGHEDCVKALVYFDLHCCRLNVQNDKGDSPLHIAARWGYEGIMEVLLENGASTLIHNKAKETPLHCALNSKILSLLERRHNDSSIRESGFESPSRSPPPSDGSSRRSSISSSSSLSIETSPLESDRSRHREVEKLLRAVADGDVEMVRYLLEWLDEDPDEEESAVPVKTELCHPLCQCANCEPTQKKAAHLHPDSVGVNSSSVDGFTPLHVAALHGHTPLISLFTHHGANLNARNNHSATPLHLACQNNHTEVVCALLECNAKLNKKDHYGNTPLIQACLKGNLEIAGVLLESGALVNLANNHGNTGLHEAVRGANVPLVELLLHRGASVHIRNNRQRTALDCAHEAAAKNSEILRLLQKAYDEAQTQRARAPEGIMARVKQSPNNGGQDFEHHKQRSLKRTDRAAATTAAAQQTAGSDRRRLTRGETLDISDPQPPSRPLTRSHTISQDLSSSVQPEEQINGGKREDEEDPSGAEADPADTEMDSVVL

Target ORF information:

RefSeq Version	XM_017354281.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354281.1

ORF Insert Sequence:

ATGGCGTTCC TCTTCCTCAT TCGTCATTTG ATGGATTTAA AGATGAAATT GTACATGGTG 
TCGAAAACTC TTCTTAACCT TCATAAATAT GAGCTGGAGT TTGGGACACA TATGGCTGTG 
TATGATGAGA ATATCCTGAA AAACCCTTTT TACCAGGCTC TGGAGAAACA CAGACCGGAC 
CTGTGCAGCC GGGTGGCAGA GCTCCATGGC ATTATCCTGG TACCGTGTTG TGGAAGTTTA 
CCAACGGGCT CTTTTACTGC AGCTCAGTTT GACGGATACG TTCTTCAACC TTCCGAGCAG 
GGTTACCAGA CTGTAGATGG AAAGGAAGTG TCTATAAACA ATAACCGGGT CCACCTGAGT 
GCAGGGTTGC CCAATCCTAT CTCTGTCCCC ATCCTGTTTG AGGAGACCTT CTACAATGAG 
AAAGAGCAGG CCTACAGTAT ACTGTGCATC GCACGGCCCT TCGAATCTGA CCACATTCCT 
GCTGAGGAGT TGGGTTCTGT TCCTGCTCCG TACTGTCTGA AAAATATTGA GGATGTGAGG 
GAGTTTTTGG GCCGCCATGC TGAAAAACTG GACAAGTTTG TTTCCTCCTT CTGTCTCTCC 
TTTAAAGAAC AGGAGAGGAA AGGCCTACGC CATCACATAG ACTCGGTGAA TACGCTTTAC 
ACCAGATGTC TTCAGTGTCT GCTGAGAGAC TCTCGTCTGA AGCTTTTGGC GAGACAGGAG 
ATTCAGATGA CTCTACTCAA AGAAGCAGTA GAGATGTACA TCCATCATGG CATCCATGAT 
CTACTCTTTA GTTATGTGGG CACTCTTGAA GCTAGTCGGG ATGCAGCGTT CAACAAAACA 
ACACGGTCCT TGCAGGATCT GCAGCAGAAA GAGCTGGGAG TGAAGTCCGA GTTCAGTATT 
AATATTCCTC GGGCGAAGCG TGAGCTGATT CAGCTGAACG TCTGCACGTC TCCACAGCAG 
AAACTCCTCT GCATCCGGAA AGTCATTTTA ACCATCATGC AGTCGACCAG ACGCAGAGAT 
ACAGCGGTGA GCATCGAGTC CATGTGTGCC GATGATCTGC TGTTGGTCAT TCTGTATCTG 
CTCATCAAGA CAGAAATTCC CAACTGGATG GCCAATCTGA GTTATATAAA GCACTTCCGC 
TTTCGGAGCT CCAGCAAGGA TGAGTTGAGT TACTGTCTGA CGTCTTTCGA GGCCGCGGTC 
GAGTATATCA GCCTGGGAAA CCTCAAACAC AACACCGATG GGTCAGGAAA CAGACTGTCC 
TTCAGGCAGA AGGTGGACCT ACTGTCCCAG AATGCAACAC CCATTGACCG GCTGTTTGAG 
CATATTGTGA GCGGAAATGA GCAGGAGGTT CAGCGTTTGC TCAGTGAAAG CGAGAGTGAG 
GAGGACGGCA GGAAGTTGTG TCACCCTCTG TGTTCCTGTG ACGCCTGCGA CCTGCGCATC 
TCTGGGAGAT TGAATGATCC TTCCATCGTC ACTCCATTCT CTCGAGACGA CAGAGGATAC 
ACTCCTTTAC ATGTTGCTGC TATTTGTGGT CAGTCTCAGA TGATTGATTT ACTGGTGTCA 
AAGGGAGCAG TGGTAAACGC TACCGATTAC CATGCACTCA CACCGCTGCA CCTCTCCTGC 
CAGAAGGGCT ACCAGGGCGT CACGCTTCTC CTGCTGCATT ATAAGGCCAA CACTGATGCT 
CAGGACAACA ATGGAAACAC TCCTCTACAT CTGGCCTGCA TGTACGGACA TGAAGACTGT 
GTGAAGGCGC TGGTGTACTT CGATCTGCAC TGCTGTCGGC TGAACGTTCA GAACGATAAA 
GGAGACTCGC CGCTGCACAT CGCCGCTCGC TGGGGATACG AGGGCATCAT GGAGGTGCTG 
CTGGAGAACG GAGCGTCCAC GCTCATCCAC AATAAAGCCA AGGAGACGCC GCTGCACTGC 
GCGCTCAACT CCAAGATTCT GTCATTGTTG GAGCGCAGAC ACAACGACTC CAGTATAAGG 
GAAAGTGGAT TCGAGTCTCC GAGCCGCTCT CCTCCTCCGT CAGACGGCAG CAGCAGACGC 
TCCTCCATCT CCAGCTCATC CTCTCTGAGT ATTGAAACCT CTCCGCTGGA GTCTGACCGC 
AGCCGGCATA GAGAGGTGGA GAAGCTGTTG AGAGCCGTGG CTGACGGAGA TGTGGAGATG 
GTGCGTTATC TCCTGGAGTG GCTGGATGAG GATCCAGATG AAGAGGAGTC TGCAGTGCCA 
GTCAAGACGG AGCTCTGCCA TCCGCTGTGC CAGTGTGCCA ACTGCGAACC CACACAGAAG 
AAGGCAGCTC ATCTTCATCC AGACAGTGTG GGTGTGAACA GCAGCAGTGT TGACGGCTTT 
ACGCCGCTGC ATGTGGCGGC GCTGCATGGA CACACACCTC TCATCTCTCT GTTCACACAC 
CACGGCGCAA ACCTGAACGC TCGCAACAAC CACAGCGCCA CCCCACTGCA CCTGGCCTGC 
CAGAACAACC ACACAGAGGT GGTCTGTGCT CTCCTGGAGT GTAATGCCAA ACTCAATAAG 
AAGGATCATT ATGGGAACAC ACCGCTCATT CAGGCCTGTC TGAAGGGGAA TCTGGAGATC 
GCCGGCGTCC TGCTGGAGAG CGGTGCGCTG GTAAATCTGG CCAATAACCA CGGGAACACG 
GGTCTGCATG AGGCTGTGAG AGGAGCAAAC GTGCCGCTGG TGGAGCTGCT GCTGCACAGA 
GGAGCTTCAG TTCACATCCG CAACAACAGA CAGAGAACAG CGCTGGACTG CGCACACGAG 
GCCGCCGCCA AGAACTCAGA GATTTTGAGA CTCCTTCAGA AAGCATATGA TGAAGCCCAG 
ACGCAAAGGG CCAGAGCACC AGAGGGCATC ATGGCAAGAG TTAAACAGAG TCCTAACAAT 
GGTGGACAGG ATTTTGAGCA CCACAAACAG AGGAGTCTGA AGAGAACAGA CAGAGCGGCA 
GCTACAACAG CAGCAGCACA ACAGACTGCA GGGAGCGACA GACGGAGACT GACCCGAGGA 
GAAACACTGG ACATCAGTGA TCCACAGCCT CCGAGCAGAC CTCTGACCCG CAGCCACACC 
ATCAGCCAAG ACCTGAGTTC CAGCGTTCAG CCCGAAGAGC AGATCAACGG TGGAAAACGG 
GAGGATGAAG AAGATCCGTC CGGTGCTGAA GCAGATCCTG CAGACACTGA GATGGACAGC 
GTGGTGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD27 ( XM_002666959.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002667005.4 Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X2, mRNA.

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CloneID	ODa57977
Clone ID Related Accession (Same CDS sequence)	XM_002666959.5 , XM_017354282.1
Accession Version	XM_002666959.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin repeat domain-containing protein 27 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002666959.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 2012 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT 
GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT 
TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC 
CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC 
TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC 
CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG 
GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC 
TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT 
ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG 
AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC 
ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC 
AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG 
TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT 
CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA 
CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT 
CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG 
CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG 
GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC 
AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG 
CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC 
GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC 
CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC 
AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA 
CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC 
CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC 
ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT 
GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG 
AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG 
GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG 
CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC 
AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC 
AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG 
TCTGACCGCA GCCGGCATAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT 
GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTGGATGAGG ATCCAGATGA AGAGGAGTCT 
GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC 
ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTGAACAG CAGCAGTGTT 
GACGGCTTTA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG 
TTCACACACC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC 
CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA 
CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT 
CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC 
GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGCCGCTGGT GGAGCTGCTG 
CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACAGC GCTGGACTGC 
GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT 
GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT 
CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC 
AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG 
ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC 
AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT 
GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG 
ATGGACAGCG TGGTGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002667005.4
CDS	142..3222
Translation	MAVYDENILKNPFYQALEKHRPDLCSRVAELHGIILVPCCGSLPTGSFTAAQFDGYVLQPSEQGYQTVDGKEVSINNNRVHLSAGLPNPISVPILFEETFYNEKEQAYSILCIARPFESDHIPAEELGSVPAPYCLKNIEDVREFLGRHAEKLDKFVSSFCLSFKEQERKGLRHHIDSVNTLYTRCLQCLLRDSRLKLLARQEIQMTLLKEAVEMYIHHGIHDLLFSYVGTLEASRDAAFNKTTRSLQDLQQKELGVKSEFSINIPRAKRELIQLNVCTSPQQKLLCIRKVILTIMQSTRRRDTAVSIESMCADDLLLVILYLLIKTEIPNWMANLSYIKHFRFRSSSKDELSYCLTSFEAAVEYISLGNLKHNTDGSGNRLSFRQKVDLLSQNATPIDRLFEHIVSGNEQEVQRLLSESESEEDGRKLCHPLCSCDACDLRISGRLNDPSIVTPFSRDDRGYTPLHVAAICGQSQMIDLLVSKGAVVNATDYHALTPLHLSCQKGYQGVTLLLLHYKANTDAQDNNGNTPLHLACMYGHEDCVKALVYFDLHCCRLNVQNDKGDSPLHIAARWGYEGIMEVLLENGASTLIHNKAKETPLHCALNSKILSLLERRHNDSSIRESGFESPSRSPPPSDGSSRRSSISSSSSLSIETSPLESDRSRHREVEKLLRAVADGDVEMVRYLLEWLDEDPDEEESAVPVKTELCHPLCQCANCEPTQKKAAHLHPDSVGVNSSSVDGFTPLHVAALHGHTPLISLFTHHGANLNARNNHSATPLHLACQNNHTEVVCALLECNAKLNKKDHYGNTPLIQACLKGNLEIAGVLLESGALVNLANNHGNTGLHEAVRGANVPLVELLLHRGASVHIRNNRQRTALDCAHEAAAKNSEILRLLQKAYDEAQTQRARAPEGIMARVKQSPNNGGQDFEHHKQRSLKRTDRAAATTAAAQQTAGSDRRRLTRGETLDISDPQPPSRPLTRSHTISQDLSSSVQPEEQINGGKREDEEDPSGAEADPADTEMDSVVL

Target ORF information:

RefSeq Version	XM_002666959.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002666959.5

ORF Insert Sequence:

ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT 
GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT 
TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC 
CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC 
TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC 
CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG 
GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC 
TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT 
ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG 
AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC 
ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC 
AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG 
TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT 
CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA 
CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT 
CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG 
CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG 
GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC 
AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG 
CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC 
GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC 
CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC 
AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA 
CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC 
CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC 
ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT 
GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG 
AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG 
GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG 
CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC 
AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC 
AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG 
TCTGACCGCA GCCGGCATAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT 
GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTGGATGAGG ATCCAGATGA AGAGGAGTCT 
GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC 
ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTGAACAG CAGCAGTGTT 
GACGGCTTTA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG 
TTCACACACC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC 
CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA 
CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT 
CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC 
GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGCCGCTGGT GGAGCTGCTG 
CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACAGC GCTGGACTGC 
GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT 
GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT 
CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC 
AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG 
ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC 
AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT 
GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG 
ATGGACAGCG TGGTGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD27 ( XM_017354282.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209771.1 Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X3, mRNA.

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CloneID	ODa57977
Clone ID Related Accession (Same CDS sequence)	XM_002666959.5 , XM_017354282.1
Accession Version	XM_017354282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin repeat domain-containing protein 27 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 2012 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT 
GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT 
TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC 
CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC 
TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC 
CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG 
GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC 
TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT 
ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG 
AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC 
ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC 
AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG 
TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT 
CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA 
CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT 
CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG 
CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG 
GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC 
AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG 
CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC 
GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC 
CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC 
AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA 
CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC 
CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC 
ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT 
GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG 
AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG 
GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG 
CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC 
AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC 
AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG 
TCTGACCGCA GCCGGCATAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT 
GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTGGATGAGG ATCCAGATGA AGAGGAGTCT 
GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC 
ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTGAACAG CAGCAGTGTT 
GACGGCTTTA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG 
TTCACACACC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC 
CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA 
CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT 
CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC 
GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGCCGCTGGT GGAGCTGCTG 
CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACAGC GCTGGACTGC 
GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT 
GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT 
CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC 
AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG 
ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC 
AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT 
GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG 
ATGGACAGCG TGGTGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209771.1
CDS	63..3143
Translation	MAVYDENILKNPFYQALEKHRPDLCSRVAELHGIILVPCCGSLPTGSFTAAQFDGYVLQPSEQGYQTVDGKEVSINNNRVHLSAGLPNPISVPILFEETFYNEKEQAYSILCIARPFESDHIPAEELGSVPAPYCLKNIEDVREFLGRHAEKLDKFVSSFCLSFKEQERKGLRHHIDSVNTLYTRCLQCLLRDSRLKLLARQEIQMTLLKEAVEMYIHHGIHDLLFSYVGTLEASRDAAFNKTTRSLQDLQQKELGVKSEFSINIPRAKRELIQLNVCTSPQQKLLCIRKVILTIMQSTRRRDTAVSIESMCADDLLLVILYLLIKTEIPNWMANLSYIKHFRFRSSSKDELSYCLTSFEAAVEYISLGNLKHNTDGSGNRLSFRQKVDLLSQNATPIDRLFEHIVSGNEQEVQRLLSESESEEDGRKLCHPLCSCDACDLRISGRLNDPSIVTPFSRDDRGYTPLHVAAICGQSQMIDLLVSKGAVVNATDYHALTPLHLSCQKGYQGVTLLLLHYKANTDAQDNNGNTPLHLACMYGHEDCVKALVYFDLHCCRLNVQNDKGDSPLHIAARWGYEGIMEVLLENGASTLIHNKAKETPLHCALNSKILSLLERRHNDSSIRESGFESPSRSPPPSDGSSRRSSISSSSSLSIETSPLESDRSRHREVEKLLRAVADGDVEMVRYLLEWLDEDPDEEESAVPVKTELCHPLCQCANCEPTQKKAAHLHPDSVGVNSSSVDGFTPLHVAALHGHTPLISLFTHHGANLNARNNHSATPLHLACQNNHTEVVCALLECNAKLNKKDHYGNTPLIQACLKGNLEIAGVLLESGALVNLANNHGNTGLHEAVRGANVPLVELLLHRGASVHIRNNRQRTALDCAHEAAAKNSEILRLLQKAYDEAQTQRARAPEGIMARVKQSPNNGGQDFEHHKQRSLKRTDRAAATTAAAQQTAGSDRRRLTRGETLDISDPQPPSRPLTRSHTISQDLSSSVQPEEQINGGKREDEEDPSGAEADPADTEMDSVVL

Target ORF information:

RefSeq Version	XM_017354282.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354282.1

ORF Insert Sequence:

ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT 
GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT 
TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC 
CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC 
TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC 
CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG 
GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC 
TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT 
ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG 
AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC 
ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC 
AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG 
TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT 
CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA 
CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT 
CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG 
CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG 
GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC 
AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG 
CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC 
GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC 
CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC 
AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA 
CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC 
CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC 
ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT 
GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG 
AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG 
GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG 
CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC 
AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC 
AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG 
TCTGACCGCA GCCGGCATAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT 
GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTGGATGAGG ATCCAGATGA AGAGGAGTCT 
GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC 
ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTGAACAG CAGCAGTGTT 
GACGGCTTTA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG 
TTCACACACC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC 
CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA 
CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT 
CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC 
GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGCCGCTGGT GGAGCTGCTG 
CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACAGC GCTGGACTGC 
GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT 
GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT 
CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC 
AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG 
ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC 
AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT 
GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG 
ATGGACAGCG TGGTGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ANKRD27 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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