synj1 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005155358.3	XP_005155415.1	synaptojanin-1 isoform X2
XM_005155359.3	XP_005155416.1	synaptojanin-1 isoform X4
XM_005155360.3	XP_005155417.1	synaptojanin-1 isoform X6
XM_005155362.3	XP_005155419.1	synaptojanin-1 isoform X7
XM_005155363.3	XP_005155420.1	synaptojanin-1 isoform X9
XM_005155363.4	XP_005155420.1	synaptojanin-1 isoform X9
XM_009305307.2	XP_009303582.1	synaptojanin-1 isoform X3
XM_005155361.3	XP_005155418.1	synaptojanin-1 isoform X5
XM_009305307.3	XP_009303582.1	synaptojanin-1 isoform X3
XM_005155360.4	XP_005155417.1	synaptojanin-1 isoform X5
XM_005155362.4	XP_005155419.1	synaptojanin-1 isoform X7
XM_021479112.1	XP_021334787.1	synaptojanin-1 isoform X12
XM_005155357.3	XP_005155414.1	synaptojanin-1 isoform X1
XM_005155357.4	XP_005155414.1	synaptojanin-1 isoform X1
XM_009305308.3	XP_009303583.1	synaptojanin-1 isoform X8
XM_005155364.4	XP_005155421.1	synaptojanin-1 isoform X10
XM_005155364.3	XP_005155421.1	synaptojanin-1 isoform X10
XM_009305308.2	XP_009303583.1	synaptojanin-1 isoform X8
XM_009305309.2	XP_009303584.1	synaptojanin-1 isoform X11
NM_001007030.2	NP_001007031.2	synaptojanin-1
XM_005155358.4	XP_005155415.1	synaptojanin-1 isoform X2
XM_005155359.4	XP_005155416.1	synaptojanin-1 isoform X4
XM_005155361.4	XP_005155418.1	synaptojanin-1 isoform X6
XM_009305309.3	XP_009303584.1	synaptojanin-1 isoform X11

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa53333	NM_001007030.2 Latest version!	Danio rerio synaptojanin 1 (synj1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa147588	XM_021479112.1 Latest version!	Danio rerio synaptojanin 1 (synj1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa16147	XM_005155357.4 Latest version!	Danio rerio synaptojanin 1 (synj1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16148	XM_005155358.4 Latest version!	Danio rerio synaptojanin 1 (synj1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16149	XM_005155359.4 Latest version!	Danio rerio synaptojanin 1 (synj1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16150	XM_005155360.4 Latest version!	Danio rerio synaptojanin 1 (synj1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16151	XM_005155361.4 Latest version!	Danio rerio synaptojanin 1 (synj1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16152	XM_005155362.4 Latest version!	Danio rerio synaptojanin 1 (synj1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16153	XM_005155363.4 Latest version!	Danio rerio synaptojanin 1 (synj1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16154	XM_005155364.4 Latest version!	Danio rerio synaptojanin 1 (synj1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa40105	XM_009305307.3 Latest version!	Danio rerio synaptojanin 1 (synj1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa40106	XM_009305308.3 Latest version!	Danio rerio synaptojanin 1 (synj1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa40107	XM_009305309.3 Latest version!	Danio rerio synaptojanin 1 (synj1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa16153	XM_005155363.3	Danio rerio synaptojanin 1 (synj1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa40105	XM_009305307.2	Danio rerio synaptojanin 1 (synj1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16150	XM_005155360.3	Danio rerio synaptojanin 1 (synj1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16152	XM_005155362.3	Danio rerio synaptojanin 1 (synj1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16147	XM_005155357.3	Danio rerio synaptojanin 1 (synj1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16154	XM_005155364.3	Danio rerio synaptojanin 1 (synj1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa40106	XM_009305308.2	Danio rerio synaptojanin 1 (synj1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16148	XM_005155358.3	Danio rerio synaptojanin 1 (synj1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16149	XM_005155359.3	Danio rerio synaptojanin 1 (synj1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa16151	XM_005155361.3	Danio rerio synaptojanin 1 (synj1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa40107	XM_009305309.2	Danio rerio synaptojanin 1 (synj1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

synj1 ( NM_001007030.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007031.2 Danio rerio synaptojanin 1 (synj1), mRNA.

Price & Availability↑

CloneID	ODa53333
Clone ID Related Accession (Same CDS sequence)	NM_001007030.2
Accession Version	NM_001007030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	synaptojanin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT027781.7. On May 24, 2016 this sequence version replaced NM_001007030.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY736013.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705, SAMN06350654 [ECO:0000348] ##Evidence-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGTTCT 
CCAGTTGATG GTCAGCCAGC AGGAGCTCCA TTCTCACAGG GACTAGAGCC AAAACGTCCC 
CCTCCGCCTC GCCCTAATGC CCCGCCAGCC CGACCTGCAC CTCCACAGCG CCCACCACCA 
CCCTCAGGTC GAGGTCAAGC CACCGGACCA GCTCCTGGAG GCATCCCAAG GCCGATCCCA 
CCCCGAGCAG GAGTCATCAG CGTCACGCCT CAGGCCAGAC CTCCTCCTCC TGCTCATCCC 
GGGGCCCCCA GACCCACAGC AGAGGTGCAT CCTGGAGCCC CTCGACCCTC ACCAGATAAT 
CATCCCGGAG CTCCAAGACC CACTGCTGAA CCCCAAAGCA AACCGTCTGA GCTGCCTCTG 
GGTCCACCCC TGACACTGCC AGGTCCCGTA AGGCCTCAGA TGACATCACC CATGCAGCCT 
CAGTCTGTGT CGCCCGTTCA GCCTCCAGTC CAACCCCAAC TGCCCCCACC CATACAATCC 
CAGCTCCCTC CACCAATGCA GCCCACCTTA CCTGCTCCTC TGATGCCTCA GCAGGCCCCT 
CAGACATCAG CCGGAGCCGG AGCCGCTGCC GCCCCTCAGC CTGGACTGGC ATCTCCCAAA 
CCTCCACCCC GCAGCCGTTC CTCTCATGCA CTGCCACCTG AATCTGCTCC AGCTCCAACA 
ACACAGCAGG AGCAATCCTC AGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007031.2
CDS	116..4042
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQQEQSSG

Target ORF information:

RefSeq Version	NM_001007030.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007030.2

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGTTCT 
CCAGTTGATG GTCAGCCAGC AGGAGCTCCA TTCTCACAGG GACTAGAGCC AAAACGTCCC 
CCTCCGCCTC GCCCTAATGC CCCGCCAGCC CGACCTGCAC CTCCACAGCG CCCACCACCA 
CCCTCAGGTC GAGGTCAAGC CACCGGACCA GCTCCTGGAG GCATCCCAAG GCCGATCCCA 
CCCCGAGCAG GAGTCATCAG CGTCACGCCT CAGGCCAGAC CTCCTCCTCC TGCTCATCCC 
GGGGCCCCCA GACCCACAGC AGAGGTGCAT CCTGGAGCCC CTCGACCCTC ACCAGATAAT 
CATCCCGGAG CTCCAAGACC CACTGCTGAA CCCCAAAGCA AACCGTCTGA GCTGCCTCTG 
GGTCCACCCC TGACACTGCC AGGTCCCGTA AGGCCTCAGA TGACATCACC CATGCAGCCT 
CAGTCTGTGT CGCCCGTTCA GCCTCCAGTC CAACCCCAAC TGCCCCCACC CATACAATCC 
CAGCTCCCTC CACCAATGCA GCCCACCTTA CCTGCTCCTC TGATGCCTCA GCAGGCCCCT 
CAGACATCAG CCGGAGCCGG AGCCGCTGCC GCCCCTCAGC CTGGACTGGC ATCTCCCAAA 
CCTCCACCCC GCAGCCGTTC CTCTCATGCA CTGCCACCTG AATCTGCTCC AGCTCCAACA 
ACACAGCAGG AGCAATCCTC AGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_021479112.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334787.1 Danio rerio synaptojanin 1 (synj1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ODa147588
Clone ID Related Accession (Same CDS sequence)	XM_021479112.1
Accession Version	XM_021479112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptojanin-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCAGA ACCACTTCAA ATACACTCGG CCCAAGAAGA TCCGAGTATG TGTGGGAACG 
TGGAACGTAA ATGGAGGCAA ACAGTTTCGC AGCATTGCGT TTCGTAACCA TACGCTCAAC 
GACTGGCTTC TGGATGCTCC TAAGAAAGCC GGACACCCAG AATTTCAGGA TGTCAAAAAC 
AACCCTGTGG ACATCTTTGC TATTGGTTTT GAGGAAATGG TGGAGCTGAA TGCTGGTAAT 
ATAGTCAGCG CAAGCACCAC AAACCAAAAG CTGTGGGCCG CAGAGCTGCA GAAGAACATC 
TCCCGAGATC AACGATATGT CCTGCTGGCC TCAGAACAGC TGGTCGGTGT GTGTCTTTTC 
GTCTTCATTC GCCCTCAGCA CGCACCCTTT ATCAGGGATG TTGCTGTAGA CACAGTGAAG 
ACTGGAATGG GCGGAGCCAC TGGTAATAAG GGAGGCGTGG CTATTCGTAT GCTCTTCCAC 
ACTACCAGCA TCTGCTTCGT GTGTTCGCAC TTTGCTGCCG GTCAATCACA AGTCAAAGAG 
AGGAACGACG ACTATAATGA GATTGCACGC AAACTGTCCT TCCCCATGGG TCGTCTTCTG 
TACTCTCATG ATTATGTATT CTGGTGTGGA GACTTCAACT ACCGAATCAA TATTCCCAAT 
GAAGAGGTGA AGGAGCTGAT CAGACAGCAG AACTGGGATG CACTTATTGG TGGAGATCAA 
CTGGTGGAGC AGAAGAATGC AGGACAGGTC TTCAGAGGCT TTATTGAAGG AAAGCTGGAT 
TTTGCGCCCA CTTATAAATA CGACCTTTTT TCTGAGGATT ACGACACTAG TGAGAAGTGC 
CGCACACCGG CCTGGACCGA CCGTGTGCTG TGGAAAAGAA GAAAGTGGAA CTTTGATAAA 
ACTGCGGAGG AGTTGGAGTT GAATGTAGTA GGAGCTCCAG TGAATGAGGA AGAACAGTAT 
CCATGGAGCC CTGGAGACCT CAAGTATTAC GGCAGAGCTG AGCTGAAAAC CTCTGATCAC 
AGACCTGTGG TGGCCATCAT AGATGTGGAC ATACTTGAGG TGGATCCCGA GGCTCGTCAT 
CAGGTGTATA AGGAAGTGAT CGCCCTGCAG GGTCCTCCCG ACGGCACCAT CCTAGTCTCT 
CTCTGCTCTT CTGGTCCCGA TGACTACTTT GATGATGCTT TAATTGACGA TCTTTTGGAC 
AAGTTTGCTA ATTTTGGCGA GGTTATTCTT ATCAGGTTTG TTGAAGAGAA AATGTGGGTG 
ACATTTTTGG AGGGATACTC AGCTCTGGCC GCTCTGTCTT TGAGTGGGTC TACTGTGAAT 
GGAAAGACCA TAGACATCCG CCTGAGGAGT CCTGGATGGA TAAAGAGTCT TGAGGAGGAA 
ATGAGTGTGG AGAGAATCTG TGGTAGTATT CCAACATCCA CCAGCTCCAC CCTGCTGGCA 
GAAAACTCTG ACCTAGGAGA AGAGTATGAC ATGGAAGGTG ATGTGGACGA GGAGGTTGAG 
GATATTTTAC CCCAGCACCT GCAGCCTGGA GCAGGCATGG ATCTGAGTGC GTCTCCTGCC 
ACATCTCCAC GCACCAGCCC ATGCCCTTCA CCCACTCACG GAGAGCCAGC ACCACCCATC 
CGACCCAGCA GAGCGCCACC ACGCACAGCC GGACCACCAC AGGGAGGATT AAGCCCAGCA 
TCCATTAGAA GGGAACTGGC AGGTTCTCCA GTTGATGGTC AGCCAGCAGG AGCTCCATTC 
TCACAGGGAC TAGAGCCAAA ACGTCCCCCT CCGCCTCGCC CTAATGCCCC GCCAGCCCGA 
CCTGCACCTC CACAGCGCCC ACCACCACCC TCAGGTGGCA GAAGTCCCAC ACCTGTTAGA 
AAAGATTCAG CAGGCTTCGC TGAGCCTCTC AATCTAGAGT TTGATCTGAC TGATAATGAA 
GGACAAAAAA GCCCTGCGTT AGCACGAGCA GATGCAGCTG GTCGAGGTCA AGCCACCGGA 
CCAGCTCCTG GAGGCATCCC AAGGCCGATC CCACCCCGAG CAGGAGTCAT CAGCGTCACG 
CCTCAGGCCA GACCTCCTCC TCCTGCTCAT CCCGGGGCCC CCAGACCCAC AGCAGAGGTG 
CATCCTGGAG CCCCTCGACC CTCACCAGAT AATCATCCCG GAGCTCCAAG ACCCACTGCT 
GAACCCCAAA GCAAACCGTC TGAGCTGCCT CTGGGTCCAC CCCTGACACT GCCAGGTCCC 
GTAAGGCCTC AGATGACATC ACCCATGCAG CCTCAGTCTG TGTCGCCCGT TCAGCCTCCA 
GTCCAACCCC AACTGCCCCC ACCCATACAA TCCCAGCTCC CTCCACCAAT GCAGCCCACC 
TTACCTGCTC CTCTGATGCC TCAGCAGGCC CCTCAGACAT CAGCCGGAGC CGGAGCCGCT 
GCCGCCCCTC AGCCTGGACT GGCATCTCCC AAACCTCCAC CCCGCAGCCG TTCCTCTCAT 
GCACTGCCAC CTGAATCTGC TCCAGCTCCA ACAACACAGG CCAAGAACAT GAATGGAGTC 
CAAAGAGAAG CACAATGGAA CCTAGACCCC TTCGACATGC TTAACCCTCA ATCCCTCTTC 
CAGAACACAC CATTCACCGC TTCCCTCTCT CGCTCCTCAT CCTCCTCTAC ATCCACTCCA 
TCCCCTTCTT CCTCATCCTC AACGTTACCC AGCAACCTTT CCCTGTTCTC AGCTCCGGAC 
ACCAGCAGCG CTTCGTGTCT CCTCGCTCCC CCCGCCCCAT CCCGCAGCAA ATCCCAAGAG 
ACCCTCCGTT GCTCCCCTAA TCCATTCCTC ACAGACATCC AACCAAGACC CAACAGCACC 
AACCCCTTCA CCAGCGGGCT GCAGTCCTCC CCGCGCCGAT CGCTTACACC TGATTTCTAC 
ACCCAAATGC AAGCCTCTAA ACCTGATTTC AATAGAGCTA TGTCTGCTGT AGGCCACAGT 
TCCACCCTTC CTCCAACGTT CTCCCGACAA CAGTCCTTAG TTACTGCTAC AGCTCCAGCC 
CCCAACAAAC AAACGCAAAA GTGGGTCACC TTTGATGATG ATTTGGATTT CCTTTCAAAA 
AGGGTTGGGA CCGGCCCATT AGCTTCACCT CTTCCTTTTC CACAAACCCA AAGCAGTTTC 
CCAAGCTCAG GCTTTGGCAT GGACAACAAC TGGGCATCGC TTCCCACCTC TGCATTCCCA 
GCAATCCCTC CGCCTGTACC AACCCGGACT AATACCAGCA TCAAAAACGC CCAACTCCCT 
TCCAGAAATG ATTTCACAGA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334787.1
CDS	287..3553
Translation	MCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_021479112.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479112.1

ORF Insert Sequence:

ATGTGTCAGA ACCACTTCAA ATACACTCGG CCCAAGAAGA TCCGAGTATG TGTGGGAACG 
TGGAACGTAA ATGGAGGCAA ACAGTTTCGC AGCATTGCGT TTCGTAACCA TACGCTCAAC 
GACTGGCTTC TGGATGCTCC TAAGAAAGCC GGACACCCAG AATTTCAGGA TGTCAAAAAC 
AACCCTGTGG ACATCTTTGC TATTGGTTTT GAGGAAATGG TGGAGCTGAA TGCTGGTAAT 
ATAGTCAGCG CAAGCACCAC AAACCAAAAG CTGTGGGCCG CAGAGCTGCA GAAGAACATC 
TCCCGAGATC AACGATATGT CCTGCTGGCC TCAGAACAGC TGGTCGGTGT GTGTCTTTTC 
GTCTTCATTC GCCCTCAGCA CGCACCCTTT ATCAGGGATG TTGCTGTAGA CACAGTGAAG 
ACTGGAATGG GCGGAGCCAC TGGTAATAAG GGAGGCGTGG CTATTCGTAT GCTCTTCCAC 
ACTACCAGCA TCTGCTTCGT GTGTTCGCAC TTTGCTGCCG GTCAATCACA AGTCAAAGAG 
AGGAACGACG ACTATAATGA GATTGCACGC AAACTGTCCT TCCCCATGGG TCGTCTTCTG 
TACTCTCATG ATTATGTATT CTGGTGTGGA GACTTCAACT ACCGAATCAA TATTCCCAAT 
GAAGAGGTGA AGGAGCTGAT CAGACAGCAG AACTGGGATG CACTTATTGG TGGAGATCAA 
CTGGTGGAGC AGAAGAATGC AGGACAGGTC TTCAGAGGCT TTATTGAAGG AAAGCTGGAT 
TTTGCGCCCA CTTATAAATA CGACCTTTTT TCTGAGGATT ACGACACTAG TGAGAAGTGC 
CGCACACCGG CCTGGACCGA CCGTGTGCTG TGGAAAAGAA GAAAGTGGAA CTTTGATAAA 
ACTGCGGAGG AGTTGGAGTT GAATGTAGTA GGAGCTCCAG TGAATGAGGA AGAACAGTAT 
CCATGGAGCC CTGGAGACCT CAAGTATTAC GGCAGAGCTG AGCTGAAAAC CTCTGATCAC 
AGACCTGTGG TGGCCATCAT AGATGTGGAC ATACTTGAGG TGGATCCCGA GGCTCGTCAT 
CAGGTGTATA AGGAAGTGAT CGCCCTGCAG GGTCCTCCCG ACGGCACCAT CCTAGTCTCT 
CTCTGCTCTT CTGGTCCCGA TGACTACTTT GATGATGCTT TAATTGACGA TCTTTTGGAC 
AAGTTTGCTA ATTTTGGCGA GGTTATTCTT ATCAGGTTTG TTGAAGAGAA AATGTGGGTG 
ACATTTTTGG AGGGATACTC AGCTCTGGCC GCTCTGTCTT TGAGTGGGTC TACTGTGAAT 
GGAAAGACCA TAGACATCCG CCTGAGGAGT CCTGGATGGA TAAAGAGTCT TGAGGAGGAA 
ATGAGTGTGG AGAGAATCTG TGGTAGTATT CCAACATCCA CCAGCTCCAC CCTGCTGGCA 
GAAAACTCTG ACCTAGGAGA AGAGTATGAC ATGGAAGGTG ATGTGGACGA GGAGGTTGAG 
GATATTTTAC CCCAGCACCT GCAGCCTGGA GCAGGCATGG ATCTGAGTGC GTCTCCTGCC 
ACATCTCCAC GCACCAGCCC ATGCCCTTCA CCCACTCACG GAGAGCCAGC ACCACCCATC 
CGACCCAGCA GAGCGCCACC ACGCACAGCC GGACCACCAC AGGGAGGATT AAGCCCAGCA 
TCCATTAGAA GGGAACTGGC AGGTTCTCCA GTTGATGGTC AGCCAGCAGG AGCTCCATTC 
TCACAGGGAC TAGAGCCAAA ACGTCCCCCT CCGCCTCGCC CTAATGCCCC GCCAGCCCGA 
CCTGCACCTC CACAGCGCCC ACCACCACCC TCAGGTGGCA GAAGTCCCAC ACCTGTTAGA 
AAAGATTCAG CAGGCTTCGC TGAGCCTCTC AATCTAGAGT TTGATCTGAC TGATAATGAA 
GGACAAAAAA GCCCTGCGTT AGCACGAGCA GATGCAGCTG GTCGAGGTCA AGCCACCGGA 
CCAGCTCCTG GAGGCATCCC AAGGCCGATC CCACCCCGAG CAGGAGTCAT CAGCGTCACG 
CCTCAGGCCA GACCTCCTCC TCCTGCTCAT CCCGGGGCCC CCAGACCCAC AGCAGAGGTG 
CATCCTGGAG CCCCTCGACC CTCACCAGAT AATCATCCCG GAGCTCCAAG ACCCACTGCT 
GAACCCCAAA GCAAACCGTC TGAGCTGCCT CTGGGTCCAC CCCTGACACT GCCAGGTCCC 
GTAAGGCCTC AGATGACATC ACCCATGCAG CCTCAGTCTG TGTCGCCCGT TCAGCCTCCA 
GTCCAACCCC AACTGCCCCC ACCCATACAA TCCCAGCTCC CTCCACCAAT GCAGCCCACC 
TTACCTGCTC CTCTGATGCC TCAGCAGGCC CCTCAGACAT CAGCCGGAGC CGGAGCCGCT 
GCCGCCCCTC AGCCTGGACT GGCATCTCCC AAACCTCCAC CCCGCAGCCG TTCCTCTCAT 
GCACTGCCAC CTGAATCTGC TCCAGCTCCA ACAACACAGG CCAAGAACAT GAATGGAGTC 
CAAAGAGAAG CACAATGGAA CCTAGACCCC TTCGACATGC TTAACCCTCA ATCCCTCTTC 
CAGAACACAC CATTCACCGC TTCCCTCTCT CGCTCCTCAT CCTCCTCTAC ATCCACTCCA 
TCCCCTTCTT CCTCATCCTC AACGTTACCC AGCAACCTTT CCCTGTTCTC AGCTCCGGAC 
ACCAGCAGCG CTTCGTGTCT CCTCGCTCCC CCCGCCCCAT CCCGCAGCAA ATCCCAAGAG 
ACCCTCCGTT GCTCCCCTAA TCCATTCCTC ACAGACATCC AACCAAGACC CAACAGCACC 
AACCCCTTCA CCAGCGGGCT GCAGTCCTCC CCGCGCCGAT CGCTTACACC TGATTTCTAC 
ACCCAAATGC AAGCCTCTAA ACCTGATTTC AATAGAGCTA TGTCTGCTGT AGGCCACAGT 
TCCACCCTTC CTCCAACGTT CTCCCGACAA CAGTCCTTAG TTACTGCTAC AGCTCCAGCC 
CCCAACAAAC AAACGCAAAA GTGGGTCACC TTTGATGATG ATTTGGATTT CCTTTCAAAA 
AGGGTTGGGA CCGGCCCATT AGCTTCACCT CTTCCTTTTC CACAAACCCA AAGCAGTTTC 
CCAAGCTCAG GCTTTGGCAT GGACAACAAC TGGGCATCGC TTCCCACCTC TGCATTCCCA 
GCAATCCCTC CGCCTGTACC AACCCGGACT AATACCAGCA TCAAAAACGC CCAACTCCCT 
TCCAGAAATG ATTTCACAGA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155357.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155414.1 Danio rerio synaptojanin 1 (synj1), transcript variant X1, mRNA.

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CloneID	ODa16147
Clone ID Related Accession (Same CDS sequence)	XM_005155357.3 , XM_005155357.4
Accession Version	XM_005155357.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synaptojanin-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155357.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGCTTC GCTGAGCCTC TCAATCTAGA GTTTGATCTG 
ACTGATAATG AAGGACAAAA AAGCCCTGCG TTAGCACGAG CAGATGCAGC TGGTCGAGGT 
CAAGCCACCG GACCAGCTCC TGGAGGCATC CCAAGGCCGA TCCCACCCCG AGCAGGAGTC 
ATCAGCGTCA CGCCTCAGGC CAGACCTCCT CCTCCTGCTC ATCCCGGGGC CCCCAGACCC 
ACAGCAGAGG TGCATCCTGG AGCCCCTCGA CCCTCACCAG ATAATCATCC CGGAGCTCCA 
AGACCCACTG CTGAACCCCA AAGCAAACCG TCTGAGCTGC CTCTGGGTCC ACCCCTGACA 
CTGCCAGGTC CCGTAAGGCC TCAGATGACA TCACCCATGC AGCCTCAGTC TGTGTCGCCC 
GTTCAGCCTC CAGTCCAACC CCAACTGCCC CCACCCATAC AATCCCAGCT CCCTCCACCA 
ATGCAGCCCA CCTTACCTGC TCCTCTGATG CCTCAGCAGG CCCCTCAGAC ATCAGCCGGA 
GCCGGAGCCG CTGCCGCCCC TCAGCCTGGA CTGGCATCTC CCAAACCTCC ACCCCGCAGC 
CGTTCCTCTC ATGCACTGCC ACCTGAATCT GCTCCAGCTC CAACAACACA GGCCAAGAAC 
ATGAATGGAG TCCAAAGAGA AGCACAATGG AACCTAGACC CCTTCGACAT GCTTAACCCT 
CAATCCCTCT TCCAGAACAC ACCATTCACC GCTTCCCTCT CTCGCTCCTC ATCCTCCTCT 
ACATCCACTC CATCCCCTTC TTCCTCATCC TCAACGTTAC CCAGCAACCT TTCCCTGTTC 
TCAGCTCCGG ACACCAGCAG CGCTTCGTGT CTCCTCGCTC CCCCCGCCCC ATCCCGCAGC 
AAATCCCAAG AGACCCTCCG TTGCTCCCCT AATCCATTCC TCACAGACAT CCAACCAAGA 
CCCAACAGCA CCAACCCCTT CACCAGCGGG CTGCAGTCCT CCCCGCGCCG ATCGCTTACA 
CCTGATTTCT ACACCCAAAT GCAAGCCTCT AAACCTGATT TCAATAGAGC TATGTCTGCT 
GTAGGCCACA GTTCCACCCT TCCTCCAACG TTCTCCCGAC AACAGTCCTT AGTTACTGCT 
ACAGCTCCAG CCCCCAACAA ACAAACGCAA AAGTGGGTCA CCTTTGATGA TGATTTGGAT 
TTCCTTTCAA AAAGGGTTGG GACCGGCCCA TTAGCTTCAC CTCTTCCTTT TCCACAAACC 
CAAAGCAGTT TCCCAAGCTC AGGCTTTGGC ATGGACAACA ACTGGGCATC GCTTCCCACC 
TCTGCATTCC CAGCAATCCC TCCGCCTGTA CCAACCCGGA CTAATACCAG CATCAAAAAC 
GCCCAACTCC CTTCCAGAAA TGATTTCACA GAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155414.1
CDS	118..4956
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_005155357.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155357.3

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGCTTC GCTGAGCCTC TCAATCTAGA GTTTGATCTG 
ACTGATAATG AAGGACAAAA AAGCCCTGCG TTAGCACGAG CAGATGCAGC TGGTCGAGGT 
CAAGCCACCG GACCAGCTCC TGGAGGCATC CCAAGGCCGA TCCCACCCCG AGCAGGAGTC 
ATCAGCGTCA CGCCTCAGGC CAGACCTCCT CCTCCTGCTC ATCCCGGGGC CCCCAGACCC 
ACAGCAGAGG TGCATCCTGG AGCCCCTCGA CCCTCACCAG ATAATCATCC CGGAGCTCCA 
AGACCCACTG CTGAACCCCA AAGCAAACCG TCTGAGCTGC CTCTGGGTCC ACCCCTGACA 
CTGCCAGGTC CCGTAAGGCC TCAGATGACA TCACCCATGC AGCCTCAGTC TGTGTCGCCC 
GTTCAGCCTC CAGTCCAACC CCAACTGCCC CCACCCATAC AATCCCAGCT CCCTCCACCA 
ATGCAGCCCA CCTTACCTGC TCCTCTGATG CCTCAGCAGG CCCCTCAGAC ATCAGCCGGA 
GCCGGAGCCG CTGCCGCCCC TCAGCCTGGA CTGGCATCTC CCAAACCTCC ACCCCGCAGC 
CGTTCCTCTC ATGCACTGCC ACCTGAATCT GCTCCAGCTC CAACAACACA GGCCAAGAAC 
ATGAATGGAG TCCAAAGAGA AGCACAATGG AACCTAGACC CCTTCGACAT GCTTAACCCT 
CAATCCCTCT TCCAGAACAC ACCATTCACC GCTTCCCTCT CTCGCTCCTC ATCCTCCTCT 
ACATCCACTC CATCCCCTTC TTCCTCATCC TCAACGTTAC CCAGCAACCT TTCCCTGTTC 
TCAGCTCCGG ACACCAGCAG CGCTTCGTGT CTCCTCGCTC CCCCCGCCCC ATCCCGCAGC 
AAATCCCAAG AGACCCTCCG TTGCTCCCCT AATCCATTCC TCACAGACAT CCAACCAAGA 
CCCAACAGCA CCAACCCCTT CACCAGCGGG CTGCAGTCCT CCCCGCGCCG ATCGCTTACA 
CCTGATTTCT ACACCCAAAT GCAAGCCTCT AAACCTGATT TCAATAGAGC TATGTCTGCT 
GTAGGCCACA GTTCCACCCT TCCTCCAACG TTCTCCCGAC AACAGTCCTT AGTTACTGCT 
ACAGCTCCAG CCCCCAACAA ACAAACGCAA AAGTGGGTCA CCTTTGATGA TGATTTGGAT 
TTCCTTTCAA AAAGGGTTGG GACCGGCCCA TTAGCTTCAC CTCTTCCTTT TCCACAAACC 
CAAAGCAGTT TCCCAAGCTC AGGCTTTGGC ATGGACAACA ACTGGGCATC GCTTCCCACC 
TCTGCATTCC CAGCAATCCC TCCGCCTGTA CCAACCCGGA CTAATACCAG CATCAAAAAC 
GCCCAACTCC CTTCCAGAAA TGATTTCACA GAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155357.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155414.1 Danio rerio synaptojanin 1 (synj1), transcript variant X1, mRNA.

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CloneID	ODa16147
Clone ID Related Accession (Same CDS sequence)	XM_005155357.3 , XM_005155357.4
Accession Version	XM_005155357.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptojanin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155357.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGCTTC GCTGAGCCTC TCAATCTAGA GTTTGATCTG 
ACTGATAATG AAGGACAAAA AAGCCCTGCG TTAGCACGAG CAGATGCAGC TGGTCGAGGT 
CAAGCCACCG GACCAGCTCC TGGAGGCATC CCAAGGCCGA TCCCACCCCG AGCAGGAGTC 
ATCAGCGTCA CGCCTCAGGC CAGACCTCCT CCTCCTGCTC ATCCCGGGGC CCCCAGACCC 
ACAGCAGAGG TGCATCCTGG AGCCCCTCGA CCCTCACCAG ATAATCATCC CGGAGCTCCA 
AGACCCACTG CTGAACCCCA AAGCAAACCG TCTGAGCTGC CTCTGGGTCC ACCCCTGACA 
CTGCCAGGTC CCGTAAGGCC TCAGATGACA TCACCCATGC AGCCTCAGTC TGTGTCGCCC 
GTTCAGCCTC CAGTCCAACC CCAACTGCCC CCACCCATAC AATCCCAGCT CCCTCCACCA 
ATGCAGCCCA CCTTACCTGC TCCTCTGATG CCTCAGCAGG CCCCTCAGAC ATCAGCCGGA 
GCCGGAGCCG CTGCCGCCCC TCAGCCTGGA CTGGCATCTC CCAAACCTCC ACCCCGCAGC 
CGTTCCTCTC ATGCACTGCC ACCTGAATCT GCTCCAGCTC CAACAACACA GGCCAAGAAC 
ATGAATGGAG TCCAAAGAGA AGCACAATGG AACCTAGACC CCTTCGACAT GCTTAACCCT 
CAATCCCTCT TCCAGAACAC ACCATTCACC GCTTCCCTCT CTCGCTCCTC ATCCTCCTCT 
ACATCCACTC CATCCCCTTC TTCCTCATCC TCAACGTTAC CCAGCAACCT TTCCCTGTTC 
TCAGCTCCGG ACACCAGCAG CGCTTCGTGT CTCCTCGCTC CCCCCGCCCC ATCCCGCAGC 
AAATCCCAAG AGACCCTCCG TTGCTCCCCT AATCCATTCC TCACAGACAT CCAACCAAGA 
CCCAACAGCA CCAACCCCTT CACCAGCGGG CTGCAGTCCT CCCCGCGCCG ATCGCTTACA 
CCTGATTTCT ACACCCAAAT GCAAGCCTCT AAACCTGATT TCAATAGAGC TATGTCTGCT 
GTAGGCCACA GTTCCACCCT TCCTCCAACG TTCTCCCGAC AACAGTCCTT AGTTACTGCT 
ACAGCTCCAG CCCCCAACAA ACAAACGCAA AAGTGGGTCA CCTTTGATGA TGATTTGGAT 
TTCCTTTCAA AAAGGGTTGG GACCGGCCCA TTAGCTTCAC CTCTTCCTTT TCCACAAACC 
CAAAGCAGTT TCCCAAGCTC AGGCTTTGGC ATGGACAACA ACTGGGCATC GCTTCCCACC 
TCTGCATTCC CAGCAATCCC TCCGCCTGTA CCAACCCGGA CTAATACCAG CATCAAAAAC 
GCCCAACTCC CTTCCAGAAA TGATTTCACA GAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155414.1
CDS	120..4958
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_005155357.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155357.4

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGCTTC GCTGAGCCTC TCAATCTAGA GTTTGATCTG 
ACTGATAATG AAGGACAAAA AAGCCCTGCG TTAGCACGAG CAGATGCAGC TGGTCGAGGT 
CAAGCCACCG GACCAGCTCC TGGAGGCATC CCAAGGCCGA TCCCACCCCG AGCAGGAGTC 
ATCAGCGTCA CGCCTCAGGC CAGACCTCCT CCTCCTGCTC ATCCCGGGGC CCCCAGACCC 
ACAGCAGAGG TGCATCCTGG AGCCCCTCGA CCCTCACCAG ATAATCATCC CGGAGCTCCA 
AGACCCACTG CTGAACCCCA AAGCAAACCG TCTGAGCTGC CTCTGGGTCC ACCCCTGACA 
CTGCCAGGTC CCGTAAGGCC TCAGATGACA TCACCCATGC AGCCTCAGTC TGTGTCGCCC 
GTTCAGCCTC CAGTCCAACC CCAACTGCCC CCACCCATAC AATCCCAGCT CCCTCCACCA 
ATGCAGCCCA CCTTACCTGC TCCTCTGATG CCTCAGCAGG CCCCTCAGAC ATCAGCCGGA 
GCCGGAGCCG CTGCCGCCCC TCAGCCTGGA CTGGCATCTC CCAAACCTCC ACCCCGCAGC 
CGTTCCTCTC ATGCACTGCC ACCTGAATCT GCTCCAGCTC CAACAACACA GGCCAAGAAC 
ATGAATGGAG TCCAAAGAGA AGCACAATGG AACCTAGACC CCTTCGACAT GCTTAACCCT 
CAATCCCTCT TCCAGAACAC ACCATTCACC GCTTCCCTCT CTCGCTCCTC ATCCTCCTCT 
ACATCCACTC CATCCCCTTC TTCCTCATCC TCAACGTTAC CCAGCAACCT TTCCCTGTTC 
TCAGCTCCGG ACACCAGCAG CGCTTCGTGT CTCCTCGCTC CCCCCGCCCC ATCCCGCAGC 
AAATCCCAAG AGACCCTCCG TTGCTCCCCT AATCCATTCC TCACAGACAT CCAACCAAGA 
CCCAACAGCA CCAACCCCTT CACCAGCGGG CTGCAGTCCT CCCCGCGCCG ATCGCTTACA 
CCTGATTTCT ACACCCAAAT GCAAGCCTCT AAACCTGATT TCAATAGAGC TATGTCTGCT 
GTAGGCCACA GTTCCACCCT TCCTCCAACG TTCTCCCGAC AACAGTCCTT AGTTACTGCT 
ACAGCTCCAG CCCCCAACAA ACAAACGCAA AAGTGGGTCA CCTTTGATGA TGATTTGGAT 
TTCCTTTCAA AAAGGGTTGG GACCGGCCCA TTAGCTTCAC CTCTTCCTTT TCCACAAACC 
CAAAGCAGTT TCCCAAGCTC AGGCTTTGGC ATGGACAACA ACTGGGCATC GCTTCCCACC 
TCTGCATTCC CAGCAATCCC TCCGCCTGTA CCAACCCGGA CTAATACCAG CATCAAAAAC 
GCCCAACTCC CTTCCAGAAA TGATTTCACA GAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155358.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155415.1 Danio rerio synaptojanin 1 (synj1), transcript variant X2, mRNA.

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CloneID	ODa16148
Clone ID Related Accession (Same CDS sequence)	XM_005155358.3 , XM_005155358.4
Accession Version	XM_005155358.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synaptojanin-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155358.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGC TAAAAGATGG AGCTCGCTCG 
GTCACCAGAA CCATCCAGAA CAACTTCTTT GACAGCTCTA AACAGGAAGC CATTGACATC 
CTGAGACTGG GCAGCACCCT TAACAGTGAC CTGGCAGATA AAGCACGAGC ACTTCTCACG 
ACAAGCAGCC TATATGTCTC TGAGCCAATT TTACAGTCAG CCTCTCCAAG GGTGCTTCTA 
GGCATGTGTC AGAACCACTT CAAATACACT CGGCCCAAGA AGATCCGAGT ATGTGTGGGA 
ACGTGGAACG TAAATGGAGG CAAACAGTTT CGCAGCATTG CGTTTCGTAA CCATACGCTC 
AACGACTGGC TTCTGGATGC TCCTAAGAAA GCCGGACACC CAGAATTTCA GGATGTCAAA 
AACAACCCTG TGGACATCTT TGCTATTGGT TTTGAGGAAA TGGTGGAGCT GAATGCTGGT 
AATATAGTCA GCGCAAGCAC CACAAACCAA AAGCTGTGGG CCGCAGAGCT GCAGAAGAAC 
ATCTCCCGAG ATCAACGATA TGTCCTGCTG GCCTCAGAAC AGCTGGTCGG TGTGTGTCTT 
TTCGTCTTCA TTCGCCCTCA GCACGCACCC TTTATCAGGG ATGTTGCTGT AGACACAGTG 
AAGACTGGAA TGGGCGGAGC CACTGGTAAT AAGGGAGGCG TGGCTATTCG TATGCTCTTC 
CACACTACCA GCATCTGCTT CGTGTGTTCG CACTTTGCTG CCGGTCAATC ACAAGTCAAA 
GAGAGGAACG ACGACTATAA TGAGATTGCA CGCAAACTGT CCTTCCCCAT GGGTCGTCTT 
CTGTACTCTC ATGATTATGT ATTCTGGTGT GGAGACTTCA ACTACCGAAT CAATATTCCC 
AATGAAGAGG TGAAGGAGCT GATCAGACAG CAGAACTGGG ATGCACTTAT TGGTGGAGAT 
CAACTGGTGG AGCAGAAGAA TGCAGGACAG GTCTTCAGAG GCTTTATTGA AGGAAAGCTG 
GATTTTGCGC CCACTTATAA ATACGACCTT TTTTCTGAGG ATTACGACAC TAGTGAGAAG 
TGCCGCACAC CGGCCTGGAC CGACCGTGTG CTGTGGAAAA GAAGAAAGTG GAACTTTGAT 
AAAACTGCGG AGGAGTTGGA GTTGAATGTA GTAGGAGCTC CAGTGAATGA GGAAGAACAG 
TATCCATGGA GCCCTGGAGA CCTCAAGTAT TACGGCAGAG CTGAGCTGAA AACCTCTGAT 
CACAGACCTG TGGTGGCCAT CATAGATGTG GACATACTTG AGGTGGATCC CGAGGCTCGT 
CATCAGGTGT ATAAGGAAGT GATCGCCCTG CAGGGTCCTC CCGACGGCAC CATCCTAGTC 
TCTCTCTGCT CTTCTGGTCC CGATGACTAC TTTGATGATG CTTTAATTGA CGATCTTTTG 
GACAAGTTTG CTAATTTTGG CGAGGTTATT CTTATCAGGT TTGTTGAAGA GAAAATGTGG 
GTGACATTTT TGGAGGGATA CTCAGCTCTG GCCGCTCTGT CTTTGAGTGG GTCTACTGTG 
AATGGAAAGA CCATAGACAT CCGCCTGAGG AGTCCTGGAT GGATAAAGAG TCTTGAGGAG 
GAAATGAGTG TGGAGAGAAT CTGTGGTAGT ATTCCAACAT CCACCAGCTC CACCCTGCTG 
GCAGAAAACT CTGACCTAGG AGAAGAGTAT GACATGGAAG GTGATGTGGA CGAGGAGGTT 
GAGGATATTT TACCCCAGCA CCTGCAGCCT GGAGCAGGCA TGGATCTGAG TGCGTCTCCT 
GCCACATCTC CACGCACCAG CCCATGCCCT TCACCCACTC ACGGAGAGCC AGCACCACCC 
ATCCGACCCA GCAGAGCGCC ACCACGCACA GCCGGACCAC CACAGGGAGG ATTAAGCCCA 
GCATCCATTA GAAGGGAACT GGCAGGTTCT CCAGTTGATG GTCAGCCAGC AGGAGCTCCA 
TTCTCACAGG GACTAGAGCC AAAACGTCCC CCTCCGCCTC GCCCTAATGC CCCGCCAGCC 
CGACCTGCAC CTCCACAGCG CCCACCACCA CCCTCAGGTG GCAGAAGTCC CACACCTGTT 
AGAAAAGATT CAGCAGGCTT CGCTGAGCCT CTCAATCTAG AGTTTGATCT GACTGATAAT 
GAAGGACAAA AAAGCCCTGC GTTAGCACGA GCAGATGCAG CTGGTCGAGG TCAAGCCACC 
GGACCAGCTC CTGGAGGCAT CCCAAGGCCG ATCCCACCCC GAGCAGGAGT CATCAGCGTC 
ACGCCTCAGG CCAGACCTCC TCCTCCTGCT CATCCCGGGG CCCCCAGACC CACAGCAGAG 
GTGCATCCTG GAGCCCCTCG ACCCTCACCA GATAATCATC CCGGAGCTCC AAGACCCACT 
GCTGAACCCC AAAGCAAACC GTCTGAGCTG CCTCTGGGTC CACCCCTGAC ACTGCCAGGT 
CCCGTAAGGC CTCAGATGAC ATCACCCATG CAGCCTCAGT CTGTGTCGCC CGTTCAGCCT 
CCAGTCCAAC CCCAACTGCC CCCACCCATA CAATCCCAGC TCCCTCCACC AATGCAGCCC 
ACCTTACCTG CTCCTCTGAT GCCTCAGCAG GCCCCTCAGA CATCAGCCGG AGCCGGAGCC 
GCTGCCGCCC CTCAGCCTGG ACTGGCATCT CCCAAACCTC CACCCCGCAG CCGTTCCTCT 
CATGCACTGC CACCTGAATC TGCTCCAGCT CCAACAACAC AGGCCAAGAA CATGAATGGA 
GTCCAAAGAG AAGCACAATG GAACCTAGAC CCCTTCGACA TGCTTAACCC TCAATCCCTC 
TTCCAGAACA CACCATTCAC CGCTTCCCTC TCTCGCTCCT CATCCTCCTC TACATCCACT 
CCATCCCCTT CTTCCTCATC CTCAACGTTA CCCAGCAACC TTTCCCTGTT CTCAGCTCCG 
GACACCAGCA GCGCTTCGTG TCTCCTCGCT CCCCCCGCCC CATCCCGCAG CAAATCCCAA 
GAGACCCTCC GTTGCTCCCC TAATCCATTC CTCACAGACA TCCAACCAAG ACCCAACAGC 
ACCAACCCCT TCACCAGCGG GCTGCAGTCC TCCCCGCGCC GATCGCTTAC ACCTGATTTC 
TACACCCAAA TGCAAGCCTC TAAACCTGAT TTCAATAGAG CTATGTCTGC TGTAGGCCAC 
AGTTCCACCC TTCCTCCAAC GTTCTCCCGA CAACAGTCCT TAGTTACTGC TACAGCTCCA 
GCCCCCAACA AACAAACGCA AAAGTGGGTC ACCTTTGATG ATGATTTGGA TTTCCTTTCA 
AAAAGGGTTG GGACCGGCCC ATTAGCTTCA CCTCTTCCTT TTCCACAAAC CCAAAGCAGT 
TTCCCAAGCT CAGGCTTTGG CATGGACAAC AACTGGGCAT CGCTTCCCAC CTCTGCATTC 
CCAGCAATCC CTCCGCCTGT ACCAACCCGG ACTAATACCA GCATCAAAAA CGCCCAACTC 
CCTTCCAGAA ATGATTTCAC AGAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155415.1
CDS	118..4947
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_005155358.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155358.3

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGC TAAAAGATGG AGCTCGCTCG 
GTCACCAGAA CCATCCAGAA CAACTTCTTT GACAGCTCTA AACAGGAAGC CATTGACATC 
CTGAGACTGG GCAGCACCCT TAACAGTGAC CTGGCAGATA AAGCACGAGC ACTTCTCACG 
ACAAGCAGCC TATATGTCTC TGAGCCAATT TTACAGTCAG CCTCTCCAAG GGTGCTTCTA 
GGCATGTGTC AGAACCACTT CAAATACACT CGGCCCAAGA AGATCCGAGT ATGTGTGGGA 
ACGTGGAACG TAAATGGAGG CAAACAGTTT CGCAGCATTG CGTTTCGTAA CCATACGCTC 
AACGACTGGC TTCTGGATGC TCCTAAGAAA GCCGGACACC CAGAATTTCA GGATGTCAAA 
AACAACCCTG TGGACATCTT TGCTATTGGT TTTGAGGAAA TGGTGGAGCT GAATGCTGGT 
AATATAGTCA GCGCAAGCAC CACAAACCAA AAGCTGTGGG CCGCAGAGCT GCAGAAGAAC 
ATCTCCCGAG ATCAACGATA TGTCCTGCTG GCCTCAGAAC AGCTGGTCGG TGTGTGTCTT 
TTCGTCTTCA TTCGCCCTCA GCACGCACCC TTTATCAGGG ATGTTGCTGT AGACACAGTG 
AAGACTGGAA TGGGCGGAGC CACTGGTAAT AAGGGAGGCG TGGCTATTCG TATGCTCTTC 
CACACTACCA GCATCTGCTT CGTGTGTTCG CACTTTGCTG CCGGTCAATC ACAAGTCAAA 
GAGAGGAACG ACGACTATAA TGAGATTGCA CGCAAACTGT CCTTCCCCAT GGGTCGTCTT 
CTGTACTCTC ATGATTATGT ATTCTGGTGT GGAGACTTCA ACTACCGAAT CAATATTCCC 
AATGAAGAGG TGAAGGAGCT GATCAGACAG CAGAACTGGG ATGCACTTAT TGGTGGAGAT 
CAACTGGTGG AGCAGAAGAA TGCAGGACAG GTCTTCAGAG GCTTTATTGA AGGAAAGCTG 
GATTTTGCGC CCACTTATAA ATACGACCTT TTTTCTGAGG ATTACGACAC TAGTGAGAAG 
TGCCGCACAC CGGCCTGGAC CGACCGTGTG CTGTGGAAAA GAAGAAAGTG GAACTTTGAT 
AAAACTGCGG AGGAGTTGGA GTTGAATGTA GTAGGAGCTC CAGTGAATGA GGAAGAACAG 
TATCCATGGA GCCCTGGAGA CCTCAAGTAT TACGGCAGAG CTGAGCTGAA AACCTCTGAT 
CACAGACCTG TGGTGGCCAT CATAGATGTG GACATACTTG AGGTGGATCC CGAGGCTCGT 
CATCAGGTGT ATAAGGAAGT GATCGCCCTG CAGGGTCCTC CCGACGGCAC CATCCTAGTC 
TCTCTCTGCT CTTCTGGTCC CGATGACTAC TTTGATGATG CTTTAATTGA CGATCTTTTG 
GACAAGTTTG CTAATTTTGG CGAGGTTATT CTTATCAGGT TTGTTGAAGA GAAAATGTGG 
GTGACATTTT TGGAGGGATA CTCAGCTCTG GCCGCTCTGT CTTTGAGTGG GTCTACTGTG 
AATGGAAAGA CCATAGACAT CCGCCTGAGG AGTCCTGGAT GGATAAAGAG TCTTGAGGAG 
GAAATGAGTG TGGAGAGAAT CTGTGGTAGT ATTCCAACAT CCACCAGCTC CACCCTGCTG 
GCAGAAAACT CTGACCTAGG AGAAGAGTAT GACATGGAAG GTGATGTGGA CGAGGAGGTT 
GAGGATATTT TACCCCAGCA CCTGCAGCCT GGAGCAGGCA TGGATCTGAG TGCGTCTCCT 
GCCACATCTC CACGCACCAG CCCATGCCCT TCACCCACTC ACGGAGAGCC AGCACCACCC 
ATCCGACCCA GCAGAGCGCC ACCACGCACA GCCGGACCAC CACAGGGAGG ATTAAGCCCA 
GCATCCATTA GAAGGGAACT GGCAGGTTCT CCAGTTGATG GTCAGCCAGC AGGAGCTCCA 
TTCTCACAGG GACTAGAGCC AAAACGTCCC CCTCCGCCTC GCCCTAATGC CCCGCCAGCC 
CGACCTGCAC CTCCACAGCG CCCACCACCA CCCTCAGGTG GCAGAAGTCC CACACCTGTT 
AGAAAAGATT CAGCAGGCTT CGCTGAGCCT CTCAATCTAG AGTTTGATCT GACTGATAAT 
GAAGGACAAA AAAGCCCTGC GTTAGCACGA GCAGATGCAG CTGGTCGAGG TCAAGCCACC 
GGACCAGCTC CTGGAGGCAT CCCAAGGCCG ATCCCACCCC GAGCAGGAGT CATCAGCGTC 
ACGCCTCAGG CCAGACCTCC TCCTCCTGCT CATCCCGGGG CCCCCAGACC CACAGCAGAG 
GTGCATCCTG GAGCCCCTCG ACCCTCACCA GATAATCATC CCGGAGCTCC AAGACCCACT 
GCTGAACCCC AAAGCAAACC GTCTGAGCTG CCTCTGGGTC CACCCCTGAC ACTGCCAGGT 
CCCGTAAGGC CTCAGATGAC ATCACCCATG CAGCCTCAGT CTGTGTCGCC CGTTCAGCCT 
CCAGTCCAAC CCCAACTGCC CCCACCCATA CAATCCCAGC TCCCTCCACC AATGCAGCCC 
ACCTTACCTG CTCCTCTGAT GCCTCAGCAG GCCCCTCAGA CATCAGCCGG AGCCGGAGCC 
GCTGCCGCCC CTCAGCCTGG ACTGGCATCT CCCAAACCTC CACCCCGCAG CCGTTCCTCT 
CATGCACTGC CACCTGAATC TGCTCCAGCT CCAACAACAC AGGCCAAGAA CATGAATGGA 
GTCCAAAGAG AAGCACAATG GAACCTAGAC CCCTTCGACA TGCTTAACCC TCAATCCCTC 
TTCCAGAACA CACCATTCAC CGCTTCCCTC TCTCGCTCCT CATCCTCCTC TACATCCACT 
CCATCCCCTT CTTCCTCATC CTCAACGTTA CCCAGCAACC TTTCCCTGTT CTCAGCTCCG 
GACACCAGCA GCGCTTCGTG TCTCCTCGCT CCCCCCGCCC CATCCCGCAG CAAATCCCAA 
GAGACCCTCC GTTGCTCCCC TAATCCATTC CTCACAGACA TCCAACCAAG ACCCAACAGC 
ACCAACCCCT TCACCAGCGG GCTGCAGTCC TCCCCGCGCC GATCGCTTAC ACCTGATTTC 
TACACCCAAA TGCAAGCCTC TAAACCTGAT TTCAATAGAG CTATGTCTGC TGTAGGCCAC 
AGTTCCACCC TTCCTCCAAC GTTCTCCCGA CAACAGTCCT TAGTTACTGC TACAGCTCCA 
GCCCCCAACA AACAAACGCA AAAGTGGGTC ACCTTTGATG ATGATTTGGA TTTCCTTTCA 
AAAAGGGTTG GGACCGGCCC ATTAGCTTCA CCTCTTCCTT TTCCACAAAC CCAAAGCAGT 
TTCCCAAGCT CAGGCTTTGG CATGGACAAC AACTGGGCAT CGCTTCCCAC CTCTGCATTC 
CCAGCAATCC CTCCGCCTGT ACCAACCCGG ACTAATACCA GCATCAAAAA CGCCCAACTC 
CCTTCCAGAA ATGATTTCAC AGAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155358.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155415.1 Danio rerio synaptojanin 1 (synj1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa16148
Clone ID Related Accession (Same CDS sequence)	XM_005155358.3 , XM_005155358.4
Accession Version	XM_005155358.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptojanin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155358.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGC TAAAAGATGG AGCTCGCTCG 
GTCACCAGAA CCATCCAGAA CAACTTCTTT GACAGCTCTA AACAGGAAGC CATTGACATC 
CTGAGACTGG GCAGCACCCT TAACAGTGAC CTGGCAGATA AAGCACGAGC ACTTCTCACG 
ACAAGCAGCC TATATGTCTC TGAGCCAATT TTACAGTCAG CCTCTCCAAG GGTGCTTCTA 
GGCATGTGTC AGAACCACTT CAAATACACT CGGCCCAAGA AGATCCGAGT ATGTGTGGGA 
ACGTGGAACG TAAATGGAGG CAAACAGTTT CGCAGCATTG CGTTTCGTAA CCATACGCTC 
AACGACTGGC TTCTGGATGC TCCTAAGAAA GCCGGACACC CAGAATTTCA GGATGTCAAA 
AACAACCCTG TGGACATCTT TGCTATTGGT TTTGAGGAAA TGGTGGAGCT GAATGCTGGT 
AATATAGTCA GCGCAAGCAC CACAAACCAA AAGCTGTGGG CCGCAGAGCT GCAGAAGAAC 
ATCTCCCGAG ATCAACGATA TGTCCTGCTG GCCTCAGAAC AGCTGGTCGG TGTGTGTCTT 
TTCGTCTTCA TTCGCCCTCA GCACGCACCC TTTATCAGGG ATGTTGCTGT AGACACAGTG 
AAGACTGGAA TGGGCGGAGC CACTGGTAAT AAGGGAGGCG TGGCTATTCG TATGCTCTTC 
CACACTACCA GCATCTGCTT CGTGTGTTCG CACTTTGCTG CCGGTCAATC ACAAGTCAAA 
GAGAGGAACG ACGACTATAA TGAGATTGCA CGCAAACTGT CCTTCCCCAT GGGTCGTCTT 
CTGTACTCTC ATGATTATGT ATTCTGGTGT GGAGACTTCA ACTACCGAAT CAATATTCCC 
AATGAAGAGG TGAAGGAGCT GATCAGACAG CAGAACTGGG ATGCACTTAT TGGTGGAGAT 
CAACTGGTGG AGCAGAAGAA TGCAGGACAG GTCTTCAGAG GCTTTATTGA AGGAAAGCTG 
GATTTTGCGC CCACTTATAA ATACGACCTT TTTTCTGAGG ATTACGACAC TAGTGAGAAG 
TGCCGCACAC CGGCCTGGAC CGACCGTGTG CTGTGGAAAA GAAGAAAGTG GAACTTTGAT 
AAAACTGCGG AGGAGTTGGA GTTGAATGTA GTAGGAGCTC CAGTGAATGA GGAAGAACAG 
TATCCATGGA GCCCTGGAGA CCTCAAGTAT TACGGCAGAG CTGAGCTGAA AACCTCTGAT 
CACAGACCTG TGGTGGCCAT CATAGATGTG GACATACTTG AGGTGGATCC CGAGGCTCGT 
CATCAGGTGT ATAAGGAAGT GATCGCCCTG CAGGGTCCTC CCGACGGCAC CATCCTAGTC 
TCTCTCTGCT CTTCTGGTCC CGATGACTAC TTTGATGATG CTTTAATTGA CGATCTTTTG 
GACAAGTTTG CTAATTTTGG CGAGGTTATT CTTATCAGGT TTGTTGAAGA GAAAATGTGG 
GTGACATTTT TGGAGGGATA CTCAGCTCTG GCCGCTCTGT CTTTGAGTGG GTCTACTGTG 
AATGGAAAGA CCATAGACAT CCGCCTGAGG AGTCCTGGAT GGATAAAGAG TCTTGAGGAG 
GAAATGAGTG TGGAGAGAAT CTGTGGTAGT ATTCCAACAT CCACCAGCTC CACCCTGCTG 
GCAGAAAACT CTGACCTAGG AGAAGAGTAT GACATGGAAG GTGATGTGGA CGAGGAGGTT 
GAGGATATTT TACCCCAGCA CCTGCAGCCT GGAGCAGGCA TGGATCTGAG TGCGTCTCCT 
GCCACATCTC CACGCACCAG CCCATGCCCT TCACCCACTC ACGGAGAGCC AGCACCACCC 
ATCCGACCCA GCAGAGCGCC ACCACGCACA GCCGGACCAC CACAGGGAGG ATTAAGCCCA 
GCATCCATTA GAAGGGAACT GGCAGGTTCT CCAGTTGATG GTCAGCCAGC AGGAGCTCCA 
TTCTCACAGG GACTAGAGCC AAAACGTCCC CCTCCGCCTC GCCCTAATGC CCCGCCAGCC 
CGACCTGCAC CTCCACAGCG CCCACCACCA CCCTCAGGTG GCAGAAGTCC CACACCTGTT 
AGAAAAGATT CAGCAGGCTT CGCTGAGCCT CTCAATCTAG AGTTTGATCT GACTGATAAT 
GAAGGACAAA AAAGCCCTGC GTTAGCACGA GCAGATGCAG CTGGTCGAGG TCAAGCCACC 
GGACCAGCTC CTGGAGGCAT CCCAAGGCCG ATCCCACCCC GAGCAGGAGT CATCAGCGTC 
ACGCCTCAGG CCAGACCTCC TCCTCCTGCT CATCCCGGGG CCCCCAGACC CACAGCAGAG 
GTGCATCCTG GAGCCCCTCG ACCCTCACCA GATAATCATC CCGGAGCTCC AAGACCCACT 
GCTGAACCCC AAAGCAAACC GTCTGAGCTG CCTCTGGGTC CACCCCTGAC ACTGCCAGGT 
CCCGTAAGGC CTCAGATGAC ATCACCCATG CAGCCTCAGT CTGTGTCGCC CGTTCAGCCT 
CCAGTCCAAC CCCAACTGCC CCCACCCATA CAATCCCAGC TCCCTCCACC AATGCAGCCC 
ACCTTACCTG CTCCTCTGAT GCCTCAGCAG GCCCCTCAGA CATCAGCCGG AGCCGGAGCC 
GCTGCCGCCC CTCAGCCTGG ACTGGCATCT CCCAAACCTC CACCCCGCAG CCGTTCCTCT 
CATGCACTGC CACCTGAATC TGCTCCAGCT CCAACAACAC AGGCCAAGAA CATGAATGGA 
GTCCAAAGAG AAGCACAATG GAACCTAGAC CCCTTCGACA TGCTTAACCC TCAATCCCTC 
TTCCAGAACA CACCATTCAC CGCTTCCCTC TCTCGCTCCT CATCCTCCTC TACATCCACT 
CCATCCCCTT CTTCCTCATC CTCAACGTTA CCCAGCAACC TTTCCCTGTT CTCAGCTCCG 
GACACCAGCA GCGCTTCGTG TCTCCTCGCT CCCCCCGCCC CATCCCGCAG CAAATCCCAA 
GAGACCCTCC GTTGCTCCCC TAATCCATTC CTCACAGACA TCCAACCAAG ACCCAACAGC 
ACCAACCCCT TCACCAGCGG GCTGCAGTCC TCCCCGCGCC GATCGCTTAC ACCTGATTTC 
TACACCCAAA TGCAAGCCTC TAAACCTGAT TTCAATAGAG CTATGTCTGC TGTAGGCCAC 
AGTTCCACCC TTCCTCCAAC GTTCTCCCGA CAACAGTCCT TAGTTACTGC TACAGCTCCA 
GCCCCCAACA AACAAACGCA AAAGTGGGTC ACCTTTGATG ATGATTTGGA TTTCCTTTCA 
AAAAGGGTTG GGACCGGCCC ATTAGCTTCA CCTCTTCCTT TTCCACAAAC CCAAAGCAGT 
TTCCCAAGCT CAGGCTTTGG CATGGACAAC AACTGGGCAT CGCTTCCCAC CTCTGCATTC 
CCAGCAATCC CTCCGCCTGT ACCAACCCGG ACTAATACCA GCATCAAAAA CGCCCAACTC 
CCTTCCAGAA ATGATTTCAC AGAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155415.1
CDS	120..4949
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_005155358.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155358.4

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGC TAAAAGATGG AGCTCGCTCG 
GTCACCAGAA CCATCCAGAA CAACTTCTTT GACAGCTCTA AACAGGAAGC CATTGACATC 
CTGAGACTGG GCAGCACCCT TAACAGTGAC CTGGCAGATA AAGCACGAGC ACTTCTCACG 
ACAAGCAGCC TATATGTCTC TGAGCCAATT TTACAGTCAG CCTCTCCAAG GGTGCTTCTA 
GGCATGTGTC AGAACCACTT CAAATACACT CGGCCCAAGA AGATCCGAGT ATGTGTGGGA 
ACGTGGAACG TAAATGGAGG CAAACAGTTT CGCAGCATTG CGTTTCGTAA CCATACGCTC 
AACGACTGGC TTCTGGATGC TCCTAAGAAA GCCGGACACC CAGAATTTCA GGATGTCAAA 
AACAACCCTG TGGACATCTT TGCTATTGGT TTTGAGGAAA TGGTGGAGCT GAATGCTGGT 
AATATAGTCA GCGCAAGCAC CACAAACCAA AAGCTGTGGG CCGCAGAGCT GCAGAAGAAC 
ATCTCCCGAG ATCAACGATA TGTCCTGCTG GCCTCAGAAC AGCTGGTCGG TGTGTGTCTT 
TTCGTCTTCA TTCGCCCTCA GCACGCACCC TTTATCAGGG ATGTTGCTGT AGACACAGTG 
AAGACTGGAA TGGGCGGAGC CACTGGTAAT AAGGGAGGCG TGGCTATTCG TATGCTCTTC 
CACACTACCA GCATCTGCTT CGTGTGTTCG CACTTTGCTG CCGGTCAATC ACAAGTCAAA 
GAGAGGAACG ACGACTATAA TGAGATTGCA CGCAAACTGT CCTTCCCCAT GGGTCGTCTT 
CTGTACTCTC ATGATTATGT ATTCTGGTGT GGAGACTTCA ACTACCGAAT CAATATTCCC 
AATGAAGAGG TGAAGGAGCT GATCAGACAG CAGAACTGGG ATGCACTTAT TGGTGGAGAT 
CAACTGGTGG AGCAGAAGAA TGCAGGACAG GTCTTCAGAG GCTTTATTGA AGGAAAGCTG 
GATTTTGCGC CCACTTATAA ATACGACCTT TTTTCTGAGG ATTACGACAC TAGTGAGAAG 
TGCCGCACAC CGGCCTGGAC CGACCGTGTG CTGTGGAAAA GAAGAAAGTG GAACTTTGAT 
AAAACTGCGG AGGAGTTGGA GTTGAATGTA GTAGGAGCTC CAGTGAATGA GGAAGAACAG 
TATCCATGGA GCCCTGGAGA CCTCAAGTAT TACGGCAGAG CTGAGCTGAA AACCTCTGAT 
CACAGACCTG TGGTGGCCAT CATAGATGTG GACATACTTG AGGTGGATCC CGAGGCTCGT 
CATCAGGTGT ATAAGGAAGT GATCGCCCTG CAGGGTCCTC CCGACGGCAC CATCCTAGTC 
TCTCTCTGCT CTTCTGGTCC CGATGACTAC TTTGATGATG CTTTAATTGA CGATCTTTTG 
GACAAGTTTG CTAATTTTGG CGAGGTTATT CTTATCAGGT TTGTTGAAGA GAAAATGTGG 
GTGACATTTT TGGAGGGATA CTCAGCTCTG GCCGCTCTGT CTTTGAGTGG GTCTACTGTG 
AATGGAAAGA CCATAGACAT CCGCCTGAGG AGTCCTGGAT GGATAAAGAG TCTTGAGGAG 
GAAATGAGTG TGGAGAGAAT CTGTGGTAGT ATTCCAACAT CCACCAGCTC CACCCTGCTG 
GCAGAAAACT CTGACCTAGG AGAAGAGTAT GACATGGAAG GTGATGTGGA CGAGGAGGTT 
GAGGATATTT TACCCCAGCA CCTGCAGCCT GGAGCAGGCA TGGATCTGAG TGCGTCTCCT 
GCCACATCTC CACGCACCAG CCCATGCCCT TCACCCACTC ACGGAGAGCC AGCACCACCC 
ATCCGACCCA GCAGAGCGCC ACCACGCACA GCCGGACCAC CACAGGGAGG ATTAAGCCCA 
GCATCCATTA GAAGGGAACT GGCAGGTTCT CCAGTTGATG GTCAGCCAGC AGGAGCTCCA 
TTCTCACAGG GACTAGAGCC AAAACGTCCC CCTCCGCCTC GCCCTAATGC CCCGCCAGCC 
CGACCTGCAC CTCCACAGCG CCCACCACCA CCCTCAGGTG GCAGAAGTCC CACACCTGTT 
AGAAAAGATT CAGCAGGCTT CGCTGAGCCT CTCAATCTAG AGTTTGATCT GACTGATAAT 
GAAGGACAAA AAAGCCCTGC GTTAGCACGA GCAGATGCAG CTGGTCGAGG TCAAGCCACC 
GGACCAGCTC CTGGAGGCAT CCCAAGGCCG ATCCCACCCC GAGCAGGAGT CATCAGCGTC 
ACGCCTCAGG CCAGACCTCC TCCTCCTGCT CATCCCGGGG CCCCCAGACC CACAGCAGAG 
GTGCATCCTG GAGCCCCTCG ACCCTCACCA GATAATCATC CCGGAGCTCC AAGACCCACT 
GCTGAACCCC AAAGCAAACC GTCTGAGCTG CCTCTGGGTC CACCCCTGAC ACTGCCAGGT 
CCCGTAAGGC CTCAGATGAC ATCACCCATG CAGCCTCAGT CTGTGTCGCC CGTTCAGCCT 
CCAGTCCAAC CCCAACTGCC CCCACCCATA CAATCCCAGC TCCCTCCACC AATGCAGCCC 
ACCTTACCTG CTCCTCTGAT GCCTCAGCAG GCCCCTCAGA CATCAGCCGG AGCCGGAGCC 
GCTGCCGCCC CTCAGCCTGG ACTGGCATCT CCCAAACCTC CACCCCGCAG CCGTTCCTCT 
CATGCACTGC CACCTGAATC TGCTCCAGCT CCAACAACAC AGGCCAAGAA CATGAATGGA 
GTCCAAAGAG AAGCACAATG GAACCTAGAC CCCTTCGACA TGCTTAACCC TCAATCCCTC 
TTCCAGAACA CACCATTCAC CGCTTCCCTC TCTCGCTCCT CATCCTCCTC TACATCCACT 
CCATCCCCTT CTTCCTCATC CTCAACGTTA CCCAGCAACC TTTCCCTGTT CTCAGCTCCG 
GACACCAGCA GCGCTTCGTG TCTCCTCGCT CCCCCCGCCC CATCCCGCAG CAAATCCCAA 
GAGACCCTCC GTTGCTCCCC TAATCCATTC CTCACAGACA TCCAACCAAG ACCCAACAGC 
ACCAACCCCT TCACCAGCGG GCTGCAGTCC TCCCCGCGCC GATCGCTTAC ACCTGATTTC 
TACACCCAAA TGCAAGCCTC TAAACCTGAT TTCAATAGAG CTATGTCTGC TGTAGGCCAC 
AGTTCCACCC TTCCTCCAAC GTTCTCCCGA CAACAGTCCT TAGTTACTGC TACAGCTCCA 
GCCCCCAACA AACAAACGCA AAAGTGGGTC ACCTTTGATG ATGATTTGGA TTTCCTTTCA 
AAAAGGGTTG GGACCGGCCC ATTAGCTTCA CCTCTTCCTT TTCCACAAAC CCAAAGCAGT 
TTCCCAAGCT CAGGCTTTGG CATGGACAAC AACTGGGCAT CGCTTCCCAC CTCTGCATTC 
CCAGCAATCC CTCCGCCTGT ACCAACCCGG ACTAATACCA GCATCAAAAA CGCCCAACTC 
CCTTCCAGAA ATGATTTCAC AGAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155359.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155416.1 Danio rerio synaptojanin 1 (synj1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa16149
Clone ID Related Accession (Same CDS sequence)	XM_005155359.3 , XM_005155359.4
Accession Version	XM_005155359.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synaptojanin-1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGTTCT 
CCAGTTGATG GTCAGCCAGC AGGAGCTCCA TTCTCACAGG GACTAGAGCC AAAACGTCCC 
CCTCCGCCTC GCCCTAATGC CCCGCCAGCC CGACCTGCAC CTCCACAGCG CCCACCACCA 
CCCTCAGGTG GCAGAAGTCC CACACCTGTT AGAAAAGATT CAGCAGGCTT CGCTGAGCCT 
CTCAATCTAG AGTTTGATCT GACTGATAAT GAAGGACAAA AAAGCCCTGC GTTAGCACGA 
GCAGATGCAG CTGGTCGAGG TCAAGCCACC GGACCAGCTC CTGGAGGCAT CCCAAGGCCG 
ATCCCACCCC GAGCAGGAGT CATCAGCGTC ACGCCTCAGG CCAGACCTCC TCCTCCTGCT 
CATCCCGGGG CCCCCAGACC CACAGCAGAG GTGCATCCTG GAGCCCCTCG ACCCTCACCA 
GATAATCATC CCGGAGCTCC AAGACCCACT GCTGAACCCC AAAGCAAACC GTCTGAGCTG 
CCTCTGGGTC CACCCCTGAC ACTGCCAGGT CCCGTAAGGC CTCAGATGAC ATCACCCATG 
CAGCCTCAGT CTGTGTCGCC CGTTCAGCCT CCAGTCCAAC CCCAACTGCC CCCACCCATA 
CAATCCCAGC TCCCTCCACC AATGCAGCCC ACCTTACCTG CTCCTCTGAT GCCTCAGCAG 
GCCCCTCAGA CATCAGCCGG AGCCGGAGCC GCTGCCGCCC CTCAGCCTGG ACTGGCATCT 
CCCAAACCTC CACCCCGCAG CCGTTCCTCT CATGCACTGC CACCTGAATC TGCTCCAGCT 
CCAACAACAC AGGCCAAGAA CATGAATGGA GTCCAAAGAG AAGCACAATG GAACCTAGAC 
CCCTTCGACA TGCTTAACCC TCAATCCCTC TTCCAGAACA CACCATTCAC CGCTTCCCTC 
TCTCGCTCCT CATCCTCCTC TACATCCACT CCATCCCCTT CTTCCTCATC CTCAACGTTA 
CCCAGCAACC TTTCCCTGTT CTCAGCTCCG GACACCAGCA GCGCTTCGTG TCTCCTCGCT 
CCCCCCGCCC CATCCCGCAG CAAATCCCAA GAGACCCTCC GTTGCTCCCC TAATCCATTC 
CTCACAGACA TCCAACCAAG ACCCAACAGC ACCAACCCCT TCACCAGCGG GCTGCAGTCC 
TCCCCGCGCC GATCGCTTAC ACCTGATTTC TACACCCAAA TGCAAGCCTC TAAACCTGAT 
TTCAATAGAG CTATGTCTGC TGTAGGCCAC AGTTCCACCC TTCCTCCAAC GTTCTCCCGA 
CAACAGTCCT TAGTTACTGC TACAGCTCCA GCCCCCAACA AACAAACGCA AAAGTGGGTC 
ACCTTTGATG ATGATTTGGA TTTCCTTTCA AAAAGGGTTG GGACCGGCCC ATTAGCTTCA 
CCTCTTCCTT TTCCACAAAC CCAAAGCAGT TTCCCAAGCT CAGGCTTTGG CATGGACAAC 
AACTGGGCAT CGCTTCCCAC CTCTGCATTC CCAGCAATCC CTCCGCCTGT ACCAACCCGG 
ACTAATACCA GCATCAAAAA CGCCCAACTC CCTTCCAGAA ATGATTTCAC AGAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155416.1
CDS	118..4917
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_005155359.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155359.3

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGTTCT 
CCAGTTGATG GTCAGCCAGC AGGAGCTCCA TTCTCACAGG GACTAGAGCC AAAACGTCCC 
CCTCCGCCTC GCCCTAATGC CCCGCCAGCC CGACCTGCAC CTCCACAGCG CCCACCACCA 
CCCTCAGGTG GCAGAAGTCC CACACCTGTT AGAAAAGATT CAGCAGGCTT CGCTGAGCCT 
CTCAATCTAG AGTTTGATCT GACTGATAAT GAAGGACAAA AAAGCCCTGC GTTAGCACGA 
GCAGATGCAG CTGGTCGAGG TCAAGCCACC GGACCAGCTC CTGGAGGCAT CCCAAGGCCG 
ATCCCACCCC GAGCAGGAGT CATCAGCGTC ACGCCTCAGG CCAGACCTCC TCCTCCTGCT 
CATCCCGGGG CCCCCAGACC CACAGCAGAG GTGCATCCTG GAGCCCCTCG ACCCTCACCA 
GATAATCATC CCGGAGCTCC AAGACCCACT GCTGAACCCC AAAGCAAACC GTCTGAGCTG 
CCTCTGGGTC CACCCCTGAC ACTGCCAGGT CCCGTAAGGC CTCAGATGAC ATCACCCATG 
CAGCCTCAGT CTGTGTCGCC CGTTCAGCCT CCAGTCCAAC CCCAACTGCC CCCACCCATA 
CAATCCCAGC TCCCTCCACC AATGCAGCCC ACCTTACCTG CTCCTCTGAT GCCTCAGCAG 
GCCCCTCAGA CATCAGCCGG AGCCGGAGCC GCTGCCGCCC CTCAGCCTGG ACTGGCATCT 
CCCAAACCTC CACCCCGCAG CCGTTCCTCT CATGCACTGC CACCTGAATC TGCTCCAGCT 
CCAACAACAC AGGCCAAGAA CATGAATGGA GTCCAAAGAG AAGCACAATG GAACCTAGAC 
CCCTTCGACA TGCTTAACCC TCAATCCCTC TTCCAGAACA CACCATTCAC CGCTTCCCTC 
TCTCGCTCCT CATCCTCCTC TACATCCACT CCATCCCCTT CTTCCTCATC CTCAACGTTA 
CCCAGCAACC TTTCCCTGTT CTCAGCTCCG GACACCAGCA GCGCTTCGTG TCTCCTCGCT 
CCCCCCGCCC CATCCCGCAG CAAATCCCAA GAGACCCTCC GTTGCTCCCC TAATCCATTC 
CTCACAGACA TCCAACCAAG ACCCAACAGC ACCAACCCCT TCACCAGCGG GCTGCAGTCC 
TCCCCGCGCC GATCGCTTAC ACCTGATTTC TACACCCAAA TGCAAGCCTC TAAACCTGAT 
TTCAATAGAG CTATGTCTGC TGTAGGCCAC AGTTCCACCC TTCCTCCAAC GTTCTCCCGA 
CAACAGTCCT TAGTTACTGC TACAGCTCCA GCCCCCAACA AACAAACGCA AAAGTGGGTC 
ACCTTTGATG ATGATTTGGA TTTCCTTTCA AAAAGGGTTG GGACCGGCCC ATTAGCTTCA 
CCTCTTCCTT TTCCACAAAC CCAAAGCAGT TTCCCAAGCT CAGGCTTTGG CATGGACAAC 
AACTGGGCAT CGCTTCCCAC CTCTGCATTC CCAGCAATCC CTCCGCCTGT ACCAACCCGG 
ACTAATACCA GCATCAAAAA CGCCCAACTC CCTTCCAGAA ATGATTTCAC AGAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155359.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155416.1 Danio rerio synaptojanin 1 (synj1), transcript variant X4, mRNA.

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CloneID	ODa16149
Clone ID Related Accession (Same CDS sequence)	XM_005155359.3 , XM_005155359.4
Accession Version	XM_005155359.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptojanin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155359.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGTTCT 
CCAGTTGATG GTCAGCCAGC AGGAGCTCCA TTCTCACAGG GACTAGAGCC AAAACGTCCC 
CCTCCGCCTC GCCCTAATGC CCCGCCAGCC CGACCTGCAC CTCCACAGCG CCCACCACCA 
CCCTCAGGTG GCAGAAGTCC CACACCTGTT AGAAAAGATT CAGCAGGCTT CGCTGAGCCT 
CTCAATCTAG AGTTTGATCT GACTGATAAT GAAGGACAAA AAAGCCCTGC GTTAGCACGA 
GCAGATGCAG CTGGTCGAGG TCAAGCCACC GGACCAGCTC CTGGAGGCAT CCCAAGGCCG 
ATCCCACCCC GAGCAGGAGT CATCAGCGTC ACGCCTCAGG CCAGACCTCC TCCTCCTGCT 
CATCCCGGGG CCCCCAGACC CACAGCAGAG GTGCATCCTG GAGCCCCTCG ACCCTCACCA 
GATAATCATC CCGGAGCTCC AAGACCCACT GCTGAACCCC AAAGCAAACC GTCTGAGCTG 
CCTCTGGGTC CACCCCTGAC ACTGCCAGGT CCCGTAAGGC CTCAGATGAC ATCACCCATG 
CAGCCTCAGT CTGTGTCGCC CGTTCAGCCT CCAGTCCAAC CCCAACTGCC CCCACCCATA 
CAATCCCAGC TCCCTCCACC AATGCAGCCC ACCTTACCTG CTCCTCTGAT GCCTCAGCAG 
GCCCCTCAGA CATCAGCCGG AGCCGGAGCC GCTGCCGCCC CTCAGCCTGG ACTGGCATCT 
CCCAAACCTC CACCCCGCAG CCGTTCCTCT CATGCACTGC CACCTGAATC TGCTCCAGCT 
CCAACAACAC AGGCCAAGAA CATGAATGGA GTCCAAAGAG AAGCACAATG GAACCTAGAC 
CCCTTCGACA TGCTTAACCC TCAATCCCTC TTCCAGAACA CACCATTCAC CGCTTCCCTC 
TCTCGCTCCT CATCCTCCTC TACATCCACT CCATCCCCTT CTTCCTCATC CTCAACGTTA 
CCCAGCAACC TTTCCCTGTT CTCAGCTCCG GACACCAGCA GCGCTTCGTG TCTCCTCGCT 
CCCCCCGCCC CATCCCGCAG CAAATCCCAA GAGACCCTCC GTTGCTCCCC TAATCCATTC 
CTCACAGACA TCCAACCAAG ACCCAACAGC ACCAACCCCT TCACCAGCGG GCTGCAGTCC 
TCCCCGCGCC GATCGCTTAC ACCTGATTTC TACACCCAAA TGCAAGCCTC TAAACCTGAT 
TTCAATAGAG CTATGTCTGC TGTAGGCCAC AGTTCCACCC TTCCTCCAAC GTTCTCCCGA 
CAACAGTCCT TAGTTACTGC TACAGCTCCA GCCCCCAACA AACAAACGCA AAAGTGGGTC 
ACCTTTGATG ATGATTTGGA TTTCCTTTCA AAAAGGGTTG GGACCGGCCC ATTAGCTTCA 
CCTCTTCCTT TTCCACAAAC CCAAAGCAGT TTCCCAAGCT CAGGCTTTGG CATGGACAAC 
AACTGGGCAT CGCTTCCCAC CTCTGCATTC CCAGCAATCC CTCCGCCTGT ACCAACCCGG 
ACTAATACCA GCATCAAAAA CGCCCAACTC CCTTCCAGAA ATGATTTCAC AGAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155416.1
CDS	120..4919
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_005155359.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155359.4

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGTTCT 
CCAGTTGATG GTCAGCCAGC AGGAGCTCCA TTCTCACAGG GACTAGAGCC AAAACGTCCC 
CCTCCGCCTC GCCCTAATGC CCCGCCAGCC CGACCTGCAC CTCCACAGCG CCCACCACCA 
CCCTCAGGTG GCAGAAGTCC CACACCTGTT AGAAAAGATT CAGCAGGCTT CGCTGAGCCT 
CTCAATCTAG AGTTTGATCT GACTGATAAT GAAGGACAAA AAAGCCCTGC GTTAGCACGA 
GCAGATGCAG CTGGTCGAGG TCAAGCCACC GGACCAGCTC CTGGAGGCAT CCCAAGGCCG 
ATCCCACCCC GAGCAGGAGT CATCAGCGTC ACGCCTCAGG CCAGACCTCC TCCTCCTGCT 
CATCCCGGGG CCCCCAGACC CACAGCAGAG GTGCATCCTG GAGCCCCTCG ACCCTCACCA 
GATAATCATC CCGGAGCTCC AAGACCCACT GCTGAACCCC AAAGCAAACC GTCTGAGCTG 
CCTCTGGGTC CACCCCTGAC ACTGCCAGGT CCCGTAAGGC CTCAGATGAC ATCACCCATG 
CAGCCTCAGT CTGTGTCGCC CGTTCAGCCT CCAGTCCAAC CCCAACTGCC CCCACCCATA 
CAATCCCAGC TCCCTCCACC AATGCAGCCC ACCTTACCTG CTCCTCTGAT GCCTCAGCAG 
GCCCCTCAGA CATCAGCCGG AGCCGGAGCC GCTGCCGCCC CTCAGCCTGG ACTGGCATCT 
CCCAAACCTC CACCCCGCAG CCGTTCCTCT CATGCACTGC CACCTGAATC TGCTCCAGCT 
CCAACAACAC AGGCCAAGAA CATGAATGGA GTCCAAAGAG AAGCACAATG GAACCTAGAC 
CCCTTCGACA TGCTTAACCC TCAATCCCTC TTCCAGAACA CACCATTCAC CGCTTCCCTC 
TCTCGCTCCT CATCCTCCTC TACATCCACT CCATCCCCTT CTTCCTCATC CTCAACGTTA 
CCCAGCAACC TTTCCCTGTT CTCAGCTCCG GACACCAGCA GCGCTTCGTG TCTCCTCGCT 
CCCCCCGCCC CATCCCGCAG CAAATCCCAA GAGACCCTCC GTTGCTCCCC TAATCCATTC 
CTCACAGACA TCCAACCAAG ACCCAACAGC ACCAACCCCT TCACCAGCGG GCTGCAGTCC 
TCCCCGCGCC GATCGCTTAC ACCTGATTTC TACACCCAAA TGCAAGCCTC TAAACCTGAT 
TTCAATAGAG CTATGTCTGC TGTAGGCCAC AGTTCCACCC TTCCTCCAAC GTTCTCCCGA 
CAACAGTCCT TAGTTACTGC TACAGCTCCA GCCCCCAACA AACAAACGCA AAAGTGGGTC 
ACCTTTGATG ATGATTTGGA TTTCCTTTCA AAAAGGGTTG GGACCGGCCC ATTAGCTTCA 
CCTCTTCCTT TTCCACAAAC CCAAAGCAGT TTCCCAAGCT CAGGCTTTGG CATGGACAAC 
AACTGGGCAT CGCTTCCCAC CTCTGCATTC CCAGCAATCC CTCCGCCTGT ACCAACCCGG 
ACTAATACCA GCATCAAAAA CGCCCAACTC CCTTCCAGAA ATGATTTCAC AGAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155360.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155417.1 Danio rerio synaptojanin 1 (synj1), transcript variant X6, mRNA.

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CloneID	ODa16150
Clone ID Related Accession (Same CDS sequence)	XM_005155360.3 , XM_005155360.4
Accession Version	XM_005155360.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synaptojanin-1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGC TAAAAGATGG AGCTCGCTCG 
GTCACCAGAA CCATCCAGAA CAACTTCTTT GACAGCTCTA AACAGGAAGC CATTGACATC 
CTGAGACTGG GCAGCACCCT TAACAGTGAC CTGGCAGATA AAGCACGAGC ACTTCTCACG 
ACAAGCAGCC TATATGTCTC TGAGCCAATT TTACAGTCAG CCTCTCCAAG GGTGCTTCTA 
GGCATGTGTC AGAACCACTT CAAATACACT CGGCCCAAGA AGATCCGAGT ATGTGTGGGA 
ACGTGGAACG TAAATGGAGG CAAACAGTTT CGCAGCATTG CGTTTCGTAA CCATACGCTC 
AACGACTGGC TTCTGGATGC TCCTAAGAAA GCCGGACACC CAGAATTTCA GGATGTCAAA 
AACAACCCTG TGGACATCTT TGCTATTGGT TTTGAGGAAA TGGTGGAGCT GAATGCTGGT 
AATATAGTCA GCGCAAGCAC CACAAACCAA AAGCTGTGGG CCGCAGAGCT GCAGAAGAAC 
ATCTCCCGAG ATCAACGATA TGTCCTGCTG GCCTCAGAAC AGCTGGTCGG TGTGTGTCTT 
TTCGTCTTCA TTCGCCCTCA GCACGCACCC TTTATCAGGG ATGTTGCTGT AGACACAGTG 
AAGACTGGAA TGGGCGGAGC CACTGGTAAT AAGGGAGGCG TGGCTATTCG TATGCTCTTC 
CACACTACCA GCATCTGCTT CGTGTGTTCG CACTTTGCTG CCGGTCAATC ACAAGTCAAA 
GAGAGGAACG ACGACTATAA TGAGATTGCA CGCAAACTGT CCTTCCCCAT GGGTCGTCTT 
CTGTACTCTC ATGATTATGT ATTCTGGTGT GGAGACTTCA ACTACCGAAT CAATATTCCC 
AATGAAGAGG TGAAGGAGCT GATCAGACAG CAGAACTGGG ATGCACTTAT TGGTGGAGAT 
CAACTGGTGG AGCAGAAGAA TGCAGGACAG GTCTTCAGAG GCTTTATTGA AGGAAAGCTG 
GATTTTGCGC CCACTTATAA ATACGACCTT TTTTCTGAGG ATTACGACAC TAGTGAGAAG 
TGCCGCACAC CGGCCTGGAC CGACCGTGTG CTGTGGAAAA GAAGAAAGTG GAACTTTGAT 
AAAACTGCGG AGGAGTTGGA GTTGAATGTA GTAGGAGCTC CAGTGAATGA GGAAGAACAG 
TATCCATGGA GCCCTGGAGA CCTCAAGTAT TACGGCAGAG CTGAGCTGAA AACCTCTGAT 
CACAGACCTG TGGTGGCCAT CATAGATGTG GACATACTTG AGGTGGATCC CGAGGCTCGT 
CATCAGGTGT ATAAGGAAGT GATCGCCCTG CAGGGTCCTC CCGACGGCAC CATCCTAGTC 
TCTCTCTGCT CTTCTGGTCC CGATGACTAC TTTGATGATG CTTTAATTGA CGATCTTTTG 
GACAAGTTTG CTAATTTTGG CGAGGTTATT CTTATCAGGT TTGTTGAAGA GAAAATGTGG 
GTGACATTTT TGGAGGGATA CTCAGCTCTG GCCGCTCTGT CTTTGAGTGG GTCTACTGTG 
AATGGAAAGA CCATAGACAT CCGCCTGAGG AGTCCTGGAT GGATAAAGAG TCTTGAGGAG 
GAAATGAGTG TGGAGAGAAT CTGTGGTAGT ATTCCAACAT CCACCAGCTC CACCCTGCTG 
GCAGAAAACT CTGACCTAGG AGAAGAGTAT GACATGGAAG GTGATGTGGA CGAGGAGGTT 
GAGGATATTT TACCCCAGCA CCTGCAGCCT GGAGCAGGCA TGGATCTGAG TGCGTCTCCT 
GCCACATCTC CACGCACCAG CCCATGCCCT TCACCCACTC ACGGAGAGCC AGCACCACCC 
ATCCGACCCA GCAGAGCGCC ACCACGCACA GCCGGACCAC CACAGGGTTC TCCAGTTGAT 
GGTCAGCCAG CAGGAGCTCC ATTCTCACAG GGACTAGAGC CAAAACGTCC CCCTCCGCCT 
CGCCCTAATG CCCCGCCAGC CCGACCTGCA CCTCCACAGC GCCCACCACC ACCCTCAGGT 
GGCAGAAGTC CCACACCTGT TAGAAAAGAT TCAGCAGGCT TCGCTGAGCC TCTCAATCTA 
GAGTTTGATC TGACTGATAA TGAAGGACAA AAAAGCCCTG CGTTAGCACG AGCAGATGCA 
GCTGGTCGAG GTCAAGCCAC CGGACCAGCT CCTGGAGGCA TCCCAAGGCC GATCCCACCC 
CGAGCAGGAG TCATCAGCGT CACGCCTCAG GCCAGACCTC CTCCTCCTGC TCATCCCGGG 
GCCCCCAGAC CCACAGCAGA GGTGCATCCT GGAGCCCCTC GACCCTCACC AGATAATCAT 
CCCGGAGCTC CAAGACCCAC TGCTGAACCC CAAAGCAAAC CGTCTGAGCT GCCTCTGGGT 
CCACCCCTGA CACTGCCAGG TCCCGTAAGG CCTCAGATGA CATCACCCAT GCAGCCTCAG 
TCTGTGTCGC CCGTTCAGCC TCCAGTCCAA CCCCAACTGC CCCCACCCAT ACAATCCCAG 
CTCCCTCCAC CAATGCAGCC CACCTTACCT GCTCCTCTGA TGCCTCAGCA GGCCCCTCAG 
ACATCAGCCG GAGCCGGAGC CGCTGCCGCC CCTCAGCCTG GACTGGCATC TCCCAAACCT 
CCACCCCGCA GCCGTTCCTC TCATGCACTG CCACCTGAAT CTGCTCCAGC TCCAACAACA 
CAGGCCAAGA ACATGAATGG AGTCCAAAGA GAAGCACAAT GGAACCTAGA CCCCTTCGAC 
ATGCTTAACC CTCAATCCCT CTTCCAGAAC ACACCATTCA CCGCTTCCCT CTCTCGCTCC 
TCATCCTCCT CTACATCCAC TCCATCCCCT TCTTCCTCAT CCTCAACGTT ACCCAGCAAC 
CTTTCCCTGT TCTCAGCTCC GGACACCAGC AGCGCTTCGT GTCTCCTCGC TCCCCCCGCC 
CCATCCCGCA GCAAATCCCA AGAGACCCTC CGTTGCTCCC CTAATCCATT CCTCACAGAC 
ATCCAACCAA GACCCAACAG CACCAACCCC TTCACCAGCG GGCTGCAGTC CTCCCCGCGC 
CGATCGCTTA CACCTGATTT CTACACCCAA ATGCAAGCCT CTAAACCTGA TTTCAATAGA 
GCTATGTCTG CTGTAGGCCA CAGTTCCACC CTTCCTCCAA CGTTCTCCCG ACAACAGTCC 
TTAGTTACTG CTACAGCTCC AGCCCCCAAC AAACAAACGC AAAAGTGGGT CACCTTTGAT 
GATGATTTGG ATTTCCTTTC AAAAAGGGTT GGGACCGGCC CATTAGCTTC ACCTCTTCCT 
TTTCCACAAA CCCAAAGCAG TTTCCCAAGC TCAGGCTTTG GCATGGACAA CAACTGGGCA 
TCGCTTCCCA CCTCTGCATT CCCAGCAATC CCTCCGCCTG TACCAACCCG GACTAATACC 
AGCATCAAAA ACGCCCAACT CCCTTCCAGA AATGATTTCA CAGAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155417.1
CDS	118..4908
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_005155360.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155360.3

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGC TAAAAGATGG AGCTCGCTCG 
GTCACCAGAA CCATCCAGAA CAACTTCTTT GACAGCTCTA AACAGGAAGC CATTGACATC 
CTGAGACTGG GCAGCACCCT TAACAGTGAC CTGGCAGATA AAGCACGAGC ACTTCTCACG 
ACAAGCAGCC TATATGTCTC TGAGCCAATT TTACAGTCAG CCTCTCCAAG GGTGCTTCTA 
GGCATGTGTC AGAACCACTT CAAATACACT CGGCCCAAGA AGATCCGAGT ATGTGTGGGA 
ACGTGGAACG TAAATGGAGG CAAACAGTTT CGCAGCATTG CGTTTCGTAA CCATACGCTC 
AACGACTGGC TTCTGGATGC TCCTAAGAAA GCCGGACACC CAGAATTTCA GGATGTCAAA 
AACAACCCTG TGGACATCTT TGCTATTGGT TTTGAGGAAA TGGTGGAGCT GAATGCTGGT 
AATATAGTCA GCGCAAGCAC CACAAACCAA AAGCTGTGGG CCGCAGAGCT GCAGAAGAAC 
ATCTCCCGAG ATCAACGATA TGTCCTGCTG GCCTCAGAAC AGCTGGTCGG TGTGTGTCTT 
TTCGTCTTCA TTCGCCCTCA GCACGCACCC TTTATCAGGG ATGTTGCTGT AGACACAGTG 
AAGACTGGAA TGGGCGGAGC CACTGGTAAT AAGGGAGGCG TGGCTATTCG TATGCTCTTC 
CACACTACCA GCATCTGCTT CGTGTGTTCG CACTTTGCTG CCGGTCAATC ACAAGTCAAA 
GAGAGGAACG ACGACTATAA TGAGATTGCA CGCAAACTGT CCTTCCCCAT GGGTCGTCTT 
CTGTACTCTC ATGATTATGT ATTCTGGTGT GGAGACTTCA ACTACCGAAT CAATATTCCC 
AATGAAGAGG TGAAGGAGCT GATCAGACAG CAGAACTGGG ATGCACTTAT TGGTGGAGAT 
CAACTGGTGG AGCAGAAGAA TGCAGGACAG GTCTTCAGAG GCTTTATTGA AGGAAAGCTG 
GATTTTGCGC CCACTTATAA ATACGACCTT TTTTCTGAGG ATTACGACAC TAGTGAGAAG 
TGCCGCACAC CGGCCTGGAC CGACCGTGTG CTGTGGAAAA GAAGAAAGTG GAACTTTGAT 
AAAACTGCGG AGGAGTTGGA GTTGAATGTA GTAGGAGCTC CAGTGAATGA GGAAGAACAG 
TATCCATGGA GCCCTGGAGA CCTCAAGTAT TACGGCAGAG CTGAGCTGAA AACCTCTGAT 
CACAGACCTG TGGTGGCCAT CATAGATGTG GACATACTTG AGGTGGATCC CGAGGCTCGT 
CATCAGGTGT ATAAGGAAGT GATCGCCCTG CAGGGTCCTC CCGACGGCAC CATCCTAGTC 
TCTCTCTGCT CTTCTGGTCC CGATGACTAC TTTGATGATG CTTTAATTGA CGATCTTTTG 
GACAAGTTTG CTAATTTTGG CGAGGTTATT CTTATCAGGT TTGTTGAAGA GAAAATGTGG 
GTGACATTTT TGGAGGGATA CTCAGCTCTG GCCGCTCTGT CTTTGAGTGG GTCTACTGTG 
AATGGAAAGA CCATAGACAT CCGCCTGAGG AGTCCTGGAT GGATAAAGAG TCTTGAGGAG 
GAAATGAGTG TGGAGAGAAT CTGTGGTAGT ATTCCAACAT CCACCAGCTC CACCCTGCTG 
GCAGAAAACT CTGACCTAGG AGAAGAGTAT GACATGGAAG GTGATGTGGA CGAGGAGGTT 
GAGGATATTT TACCCCAGCA CCTGCAGCCT GGAGCAGGCA TGGATCTGAG TGCGTCTCCT 
GCCACATCTC CACGCACCAG CCCATGCCCT TCACCCACTC ACGGAGAGCC AGCACCACCC 
ATCCGACCCA GCAGAGCGCC ACCACGCACA GCCGGACCAC CACAGGGTTC TCCAGTTGAT 
GGTCAGCCAG CAGGAGCTCC ATTCTCACAG GGACTAGAGC CAAAACGTCC CCCTCCGCCT 
CGCCCTAATG CCCCGCCAGC CCGACCTGCA CCTCCACAGC GCCCACCACC ACCCTCAGGT 
GGCAGAAGTC CCACACCTGT TAGAAAAGAT TCAGCAGGCT TCGCTGAGCC TCTCAATCTA 
GAGTTTGATC TGACTGATAA TGAAGGACAA AAAAGCCCTG CGTTAGCACG AGCAGATGCA 
GCTGGTCGAG GTCAAGCCAC CGGACCAGCT CCTGGAGGCA TCCCAAGGCC GATCCCACCC 
CGAGCAGGAG TCATCAGCGT CACGCCTCAG GCCAGACCTC CTCCTCCTGC TCATCCCGGG 
GCCCCCAGAC CCACAGCAGA GGTGCATCCT GGAGCCCCTC GACCCTCACC AGATAATCAT 
CCCGGAGCTC CAAGACCCAC TGCTGAACCC CAAAGCAAAC CGTCTGAGCT GCCTCTGGGT 
CCACCCCTGA CACTGCCAGG TCCCGTAAGG CCTCAGATGA CATCACCCAT GCAGCCTCAG 
TCTGTGTCGC CCGTTCAGCC TCCAGTCCAA CCCCAACTGC CCCCACCCAT ACAATCCCAG 
CTCCCTCCAC CAATGCAGCC CACCTTACCT GCTCCTCTGA TGCCTCAGCA GGCCCCTCAG 
ACATCAGCCG GAGCCGGAGC CGCTGCCGCC CCTCAGCCTG GACTGGCATC TCCCAAACCT 
CCACCCCGCA GCCGTTCCTC TCATGCACTG CCACCTGAAT CTGCTCCAGC TCCAACAACA 
CAGGCCAAGA ACATGAATGG AGTCCAAAGA GAAGCACAAT GGAACCTAGA CCCCTTCGAC 
ATGCTTAACC CTCAATCCCT CTTCCAGAAC ACACCATTCA CCGCTTCCCT CTCTCGCTCC 
TCATCCTCCT CTACATCCAC TCCATCCCCT TCTTCCTCAT CCTCAACGTT ACCCAGCAAC 
CTTTCCCTGT TCTCAGCTCC GGACACCAGC AGCGCTTCGT GTCTCCTCGC TCCCCCCGCC 
CCATCCCGCA GCAAATCCCA AGAGACCCTC CGTTGCTCCC CTAATCCATT CCTCACAGAC 
ATCCAACCAA GACCCAACAG CACCAACCCC TTCACCAGCG GGCTGCAGTC CTCCCCGCGC 
CGATCGCTTA CACCTGATTT CTACACCCAA ATGCAAGCCT CTAAACCTGA TTTCAATAGA 
GCTATGTCTG CTGTAGGCCA CAGTTCCACC CTTCCTCCAA CGTTCTCCCG ACAACAGTCC 
TTAGTTACTG CTACAGCTCC AGCCCCCAAC AAACAAACGC AAAAGTGGGT CACCTTTGAT 
GATGATTTGG ATTTCCTTTC AAAAAGGGTT GGGACCGGCC CATTAGCTTC ACCTCTTCCT 
TTTCCACAAA CCCAAAGCAG TTTCCCAAGC TCAGGCTTTG GCATGGACAA CAACTGGGCA 
TCGCTTCCCA CCTCTGCATT CCCAGCAATC CCTCCGCCTG TACCAACCCG GACTAATACC 
AGCATCAAAA ACGCCCAACT CCCTTCCAGA AATGATTTCA CAGAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155360.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155417.1 Danio rerio synaptojanin 1 (synj1), transcript variant X5, mRNA.

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CloneID	ODa16150
Clone ID Related Accession (Same CDS sequence)	XM_005155360.3 , XM_005155360.4
Accession Version	XM_005155360.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptojanin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155360.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGC TAAAAGATGG AGCTCGCTCG 
GTCACCAGAA CCATCCAGAA CAACTTCTTT GACAGCTCTA AACAGGAAGC CATTGACATC 
CTGAGACTGG GCAGCACCCT TAACAGTGAC CTGGCAGATA AAGCACGAGC ACTTCTCACG 
ACAAGCAGCC TATATGTCTC TGAGCCAATT TTACAGTCAG CCTCTCCAAG GGTGCTTCTA 
GGCATGTGTC AGAACCACTT CAAATACACT CGGCCCAAGA AGATCCGAGT ATGTGTGGGA 
ACGTGGAACG TAAATGGAGG CAAACAGTTT CGCAGCATTG CGTTTCGTAA CCATACGCTC 
AACGACTGGC TTCTGGATGC TCCTAAGAAA GCCGGACACC CAGAATTTCA GGATGTCAAA 
AACAACCCTG TGGACATCTT TGCTATTGGT TTTGAGGAAA TGGTGGAGCT GAATGCTGGT 
AATATAGTCA GCGCAAGCAC CACAAACCAA AAGCTGTGGG CCGCAGAGCT GCAGAAGAAC 
ATCTCCCGAG ATCAACGATA TGTCCTGCTG GCCTCAGAAC AGCTGGTCGG TGTGTGTCTT 
TTCGTCTTCA TTCGCCCTCA GCACGCACCC TTTATCAGGG ATGTTGCTGT AGACACAGTG 
AAGACTGGAA TGGGCGGAGC CACTGGTAAT AAGGGAGGCG TGGCTATTCG TATGCTCTTC 
CACACTACCA GCATCTGCTT CGTGTGTTCG CACTTTGCTG CCGGTCAATC ACAAGTCAAA 
GAGAGGAACG ACGACTATAA TGAGATTGCA CGCAAACTGT CCTTCCCCAT GGGTCGTCTT 
CTGTACTCTC ATGATTATGT ATTCTGGTGT GGAGACTTCA ACTACCGAAT CAATATTCCC 
AATGAAGAGG TGAAGGAGCT GATCAGACAG CAGAACTGGG ATGCACTTAT TGGTGGAGAT 
CAACTGGTGG AGCAGAAGAA TGCAGGACAG GTCTTCAGAG GCTTTATTGA AGGAAAGCTG 
GATTTTGCGC CCACTTATAA ATACGACCTT TTTTCTGAGG ATTACGACAC TAGTGAGAAG 
TGCCGCACAC CGGCCTGGAC CGACCGTGTG CTGTGGAAAA GAAGAAAGTG GAACTTTGAT 
AAAACTGCGG AGGAGTTGGA GTTGAATGTA GTAGGAGCTC CAGTGAATGA GGAAGAACAG 
TATCCATGGA GCCCTGGAGA CCTCAAGTAT TACGGCAGAG CTGAGCTGAA AACCTCTGAT 
CACAGACCTG TGGTGGCCAT CATAGATGTG GACATACTTG AGGTGGATCC CGAGGCTCGT 
CATCAGGTGT ATAAGGAAGT GATCGCCCTG CAGGGTCCTC CCGACGGCAC CATCCTAGTC 
TCTCTCTGCT CTTCTGGTCC CGATGACTAC TTTGATGATG CTTTAATTGA CGATCTTTTG 
GACAAGTTTG CTAATTTTGG CGAGGTTATT CTTATCAGGT TTGTTGAAGA GAAAATGTGG 
GTGACATTTT TGGAGGGATA CTCAGCTCTG GCCGCTCTGT CTTTGAGTGG GTCTACTGTG 
AATGGAAAGA CCATAGACAT CCGCCTGAGG AGTCCTGGAT GGATAAAGAG TCTTGAGGAG 
GAAATGAGTG TGGAGAGAAT CTGTGGTAGT ATTCCAACAT CCACCAGCTC CACCCTGCTG 
GCAGAAAACT CTGACCTAGG AGAAGAGTAT GACATGGAAG GTGATGTGGA CGAGGAGGTT 
GAGGATATTT TACCCCAGCA CCTGCAGCCT GGAGCAGGCA TGGATCTGAG TGCGTCTCCT 
GCCACATCTC CACGCACCAG CCCATGCCCT TCACCCACTC ACGGAGAGCC AGCACCACCC 
ATCCGACCCA GCAGAGCGCC ACCACGCACA GCCGGACCAC CACAGGGTTC TCCAGTTGAT 
GGTCAGCCAG CAGGAGCTCC ATTCTCACAG GGACTAGAGC CAAAACGTCC CCCTCCGCCT 
CGCCCTAATG CCCCGCCAGC CCGACCTGCA CCTCCACAGC GCCCACCACC ACCCTCAGGT 
GGCAGAAGTC CCACACCTGT TAGAAAAGAT TCAGCAGGCT TCGCTGAGCC TCTCAATCTA 
GAGTTTGATC TGACTGATAA TGAAGGACAA AAAAGCCCTG CGTTAGCACG AGCAGATGCA 
GCTGGTCGAG GTCAAGCCAC CGGACCAGCT CCTGGAGGCA TCCCAAGGCC GATCCCACCC 
CGAGCAGGAG TCATCAGCGT CACGCCTCAG GCCAGACCTC CTCCTCCTGC TCATCCCGGG 
GCCCCCAGAC CCACAGCAGA GGTGCATCCT GGAGCCCCTC GACCCTCACC AGATAATCAT 
CCCGGAGCTC CAAGACCCAC TGCTGAACCC CAAAGCAAAC CGTCTGAGCT GCCTCTGGGT 
CCACCCCTGA CACTGCCAGG TCCCGTAAGG CCTCAGATGA CATCACCCAT GCAGCCTCAG 
TCTGTGTCGC CCGTTCAGCC TCCAGTCCAA CCCCAACTGC CCCCACCCAT ACAATCCCAG 
CTCCCTCCAC CAATGCAGCC CACCTTACCT GCTCCTCTGA TGCCTCAGCA GGCCCCTCAG 
ACATCAGCCG GAGCCGGAGC CGCTGCCGCC CCTCAGCCTG GACTGGCATC TCCCAAACCT 
CCACCCCGCA GCCGTTCCTC TCATGCACTG CCACCTGAAT CTGCTCCAGC TCCAACAACA 
CAGGCCAAGA ACATGAATGG AGTCCAAAGA GAAGCACAAT GGAACCTAGA CCCCTTCGAC 
ATGCTTAACC CTCAATCCCT CTTCCAGAAC ACACCATTCA CCGCTTCCCT CTCTCGCTCC 
TCATCCTCCT CTACATCCAC TCCATCCCCT TCTTCCTCAT CCTCAACGTT ACCCAGCAAC 
CTTTCCCTGT TCTCAGCTCC GGACACCAGC AGCGCTTCGT GTCTCCTCGC TCCCCCCGCC 
CCATCCCGCA GCAAATCCCA AGAGACCCTC CGTTGCTCCC CTAATCCATT CCTCACAGAC 
ATCCAACCAA GACCCAACAG CACCAACCCC TTCACCAGCG GGCTGCAGTC CTCCCCGCGC 
CGATCGCTTA CACCTGATTT CTACACCCAA ATGCAAGCCT CTAAACCTGA TTTCAATAGA 
GCTATGTCTG CTGTAGGCCA CAGTTCCACC CTTCCTCCAA CGTTCTCCCG ACAACAGTCC 
TTAGTTACTG CTACAGCTCC AGCCCCCAAC AAACAAACGC AAAAGTGGGT CACCTTTGAT 
GATGATTTGG ATTTCCTTTC AAAAAGGGTT GGGACCGGCC CATTAGCTTC ACCTCTTCCT 
TTTCCACAAA CCCAAAGCAG TTTCCCAAGC TCAGGCTTTG GCATGGACAA CAACTGGGCA 
TCGCTTCCCA CCTCTGCATT CCCAGCAATC CCTCCGCCTG TACCAACCCG GACTAATACC 
AGCATCAAAA ACGCCCAACT CCCTTCCAGA AATGATTTCA CAGAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155417.1
CDS	120..4910
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_005155360.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155360.4

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGC TAAAAGATGG AGCTCGCTCG 
GTCACCAGAA CCATCCAGAA CAACTTCTTT GACAGCTCTA AACAGGAAGC CATTGACATC 
CTGAGACTGG GCAGCACCCT TAACAGTGAC CTGGCAGATA AAGCACGAGC ACTTCTCACG 
ACAAGCAGCC TATATGTCTC TGAGCCAATT TTACAGTCAG CCTCTCCAAG GGTGCTTCTA 
GGCATGTGTC AGAACCACTT CAAATACACT CGGCCCAAGA AGATCCGAGT ATGTGTGGGA 
ACGTGGAACG TAAATGGAGG CAAACAGTTT CGCAGCATTG CGTTTCGTAA CCATACGCTC 
AACGACTGGC TTCTGGATGC TCCTAAGAAA GCCGGACACC CAGAATTTCA GGATGTCAAA 
AACAACCCTG TGGACATCTT TGCTATTGGT TTTGAGGAAA TGGTGGAGCT GAATGCTGGT 
AATATAGTCA GCGCAAGCAC CACAAACCAA AAGCTGTGGG CCGCAGAGCT GCAGAAGAAC 
ATCTCCCGAG ATCAACGATA TGTCCTGCTG GCCTCAGAAC AGCTGGTCGG TGTGTGTCTT 
TTCGTCTTCA TTCGCCCTCA GCACGCACCC TTTATCAGGG ATGTTGCTGT AGACACAGTG 
AAGACTGGAA TGGGCGGAGC CACTGGTAAT AAGGGAGGCG TGGCTATTCG TATGCTCTTC 
CACACTACCA GCATCTGCTT CGTGTGTTCG CACTTTGCTG CCGGTCAATC ACAAGTCAAA 
GAGAGGAACG ACGACTATAA TGAGATTGCA CGCAAACTGT CCTTCCCCAT GGGTCGTCTT 
CTGTACTCTC ATGATTATGT ATTCTGGTGT GGAGACTTCA ACTACCGAAT CAATATTCCC 
AATGAAGAGG TGAAGGAGCT GATCAGACAG CAGAACTGGG ATGCACTTAT TGGTGGAGAT 
CAACTGGTGG AGCAGAAGAA TGCAGGACAG GTCTTCAGAG GCTTTATTGA AGGAAAGCTG 
GATTTTGCGC CCACTTATAA ATACGACCTT TTTTCTGAGG ATTACGACAC TAGTGAGAAG 
TGCCGCACAC CGGCCTGGAC CGACCGTGTG CTGTGGAAAA GAAGAAAGTG GAACTTTGAT 
AAAACTGCGG AGGAGTTGGA GTTGAATGTA GTAGGAGCTC CAGTGAATGA GGAAGAACAG 
TATCCATGGA GCCCTGGAGA CCTCAAGTAT TACGGCAGAG CTGAGCTGAA AACCTCTGAT 
CACAGACCTG TGGTGGCCAT CATAGATGTG GACATACTTG AGGTGGATCC CGAGGCTCGT 
CATCAGGTGT ATAAGGAAGT GATCGCCCTG CAGGGTCCTC CCGACGGCAC CATCCTAGTC 
TCTCTCTGCT CTTCTGGTCC CGATGACTAC TTTGATGATG CTTTAATTGA CGATCTTTTG 
GACAAGTTTG CTAATTTTGG CGAGGTTATT CTTATCAGGT TTGTTGAAGA GAAAATGTGG 
GTGACATTTT TGGAGGGATA CTCAGCTCTG GCCGCTCTGT CTTTGAGTGG GTCTACTGTG 
AATGGAAAGA CCATAGACAT CCGCCTGAGG AGTCCTGGAT GGATAAAGAG TCTTGAGGAG 
GAAATGAGTG TGGAGAGAAT CTGTGGTAGT ATTCCAACAT CCACCAGCTC CACCCTGCTG 
GCAGAAAACT CTGACCTAGG AGAAGAGTAT GACATGGAAG GTGATGTGGA CGAGGAGGTT 
GAGGATATTT TACCCCAGCA CCTGCAGCCT GGAGCAGGCA TGGATCTGAG TGCGTCTCCT 
GCCACATCTC CACGCACCAG CCCATGCCCT TCACCCACTC ACGGAGAGCC AGCACCACCC 
ATCCGACCCA GCAGAGCGCC ACCACGCACA GCCGGACCAC CACAGGGTTC TCCAGTTGAT 
GGTCAGCCAG CAGGAGCTCC ATTCTCACAG GGACTAGAGC CAAAACGTCC CCCTCCGCCT 
CGCCCTAATG CCCCGCCAGC CCGACCTGCA CCTCCACAGC GCCCACCACC ACCCTCAGGT 
GGCAGAAGTC CCACACCTGT TAGAAAAGAT TCAGCAGGCT TCGCTGAGCC TCTCAATCTA 
GAGTTTGATC TGACTGATAA TGAAGGACAA AAAAGCCCTG CGTTAGCACG AGCAGATGCA 
GCTGGTCGAG GTCAAGCCAC CGGACCAGCT CCTGGAGGCA TCCCAAGGCC GATCCCACCC 
CGAGCAGGAG TCATCAGCGT CACGCCTCAG GCCAGACCTC CTCCTCCTGC TCATCCCGGG 
GCCCCCAGAC CCACAGCAGA GGTGCATCCT GGAGCCCCTC GACCCTCACC AGATAATCAT 
CCCGGAGCTC CAAGACCCAC TGCTGAACCC CAAAGCAAAC CGTCTGAGCT GCCTCTGGGT 
CCACCCCTGA CACTGCCAGG TCCCGTAAGG CCTCAGATGA CATCACCCAT GCAGCCTCAG 
TCTGTGTCGC CCGTTCAGCC TCCAGTCCAA CCCCAACTGC CCCCACCCAT ACAATCCCAG 
CTCCCTCCAC CAATGCAGCC CACCTTACCT GCTCCTCTGA TGCCTCAGCA GGCCCCTCAG 
ACATCAGCCG GAGCCGGAGC CGCTGCCGCC CCTCAGCCTG GACTGGCATC TCCCAAACCT 
CCACCCCGCA GCCGTTCCTC TCATGCACTG CCACCTGAAT CTGCTCCAGC TCCAACAACA 
CAGGCCAAGA ACATGAATGG AGTCCAAAGA GAAGCACAAT GGAACCTAGA CCCCTTCGAC 
ATGCTTAACC CTCAATCCCT CTTCCAGAAC ACACCATTCA CCGCTTCCCT CTCTCGCTCC 
TCATCCTCCT CTACATCCAC TCCATCCCCT TCTTCCTCAT CCTCAACGTT ACCCAGCAAC 
CTTTCCCTGT TCTCAGCTCC GGACACCAGC AGCGCTTCGT GTCTCCTCGC TCCCCCCGCC 
CCATCCCGCA GCAAATCCCA AGAGACCCTC CGTTGCTCCC CTAATCCATT CCTCACAGAC 
ATCCAACCAA GACCCAACAG CACCAACCCC TTCACCAGCG GGCTGCAGTC CTCCCCGCGC 
CGATCGCTTA CACCTGATTT CTACACCCAA ATGCAAGCCT CTAAACCTGA TTTCAATAGA 
GCTATGTCTG CTGTAGGCCA CAGTTCCACC CTTCCTCCAA CGTTCTCCCG ACAACAGTCC 
TTAGTTACTG CTACAGCTCC AGCCCCCAAC AAACAAACGC AAAAGTGGGT CACCTTTGAT 
GATGATTTGG ATTTCCTTTC AAAAAGGGTT GGGACCGGCC CATTAGCTTC ACCTCTTCCT 
TTTCCACAAA CCCAAAGCAG TTTCCCAAGC TCAGGCTTTG GCATGGACAA CAACTGGGCA 
TCGCTTCCCA CCTCTGCATT CCCAGCAATC CCTCCGCCTG TACCAACCCG GACTAATACC 
AGCATCAAAA ACGCCCAACT CCCTTCCAGA AATGATTTCA CAGAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155361.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155418.1 Danio rerio synaptojanin 1 (synj1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa16151
Clone ID Related Accession (Same CDS sequence)	XM_005155361.3 , XM_005155361.4
Accession Version	XM_005155361.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synaptojanin-1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155361.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGACAA AAAAGCCCTG CGTTAGCACG AGCAGATGCA 
GCTGGTCGAG GTCAAGCCAC CGGACCAGCT CCTGGAGGCA TCCCAAGGCC GATCCCACCC 
CGAGCAGGAG TCATCAGCGT CACGCCTCAG GCCAGACCTC CTCCTCCTGC TCATCCCGGG 
GCCCCCAGAC CCACAGCAGA GGTGCATCCT GGAGCCCCTC GACCCTCACC AGATAATCAT 
CCCGGAGCTC CAAGACCCAC TGCTGAACCC CAAAGCAAAC CGTCTGAGCT GCCTCTGGGT 
CCACCCCTGA CACTGCCAGG TCCCGTAAGG CCTCAGATGA CATCACCCAT GCAGCCTCAG 
TCTGTGTCGC CCGTTCAGCC TCCAGTCCAA CCCCAACTGC CCCCACCCAT ACAATCCCAG 
CTCCCTCCAC CAATGCAGCC CACCTTACCT GCTCCTCTGA TGCCTCAGCA GGCCCCTCAG 
ACATCAGCCG GAGCCGGAGC CGCTGCCGCC CCTCAGCCTG GACTGGCATC TCCCAAACCT 
CCACCCCGCA GCCGTTCCTC TCATGCACTG CCACCTGAAT CTGCTCCAGC TCCAACAACA 
CAGGCCAAGA ACATGAATGG AGTCCAAAGA GAAGCACAAT GGAACCTAGA CCCCTTCGAC 
ATGCTTAACC CTCAATCCCT CTTCCAGAAC ACACCATTCA CCGCTTCCCT CTCTCGCTCC 
TCATCCTCCT CTACATCCAC TCCATCCCCT TCTTCCTCAT CCTCAACGTT ACCCAGCAAC 
CTTTCCCTGT TCTCAGCTCC GGACACCAGC AGCGCTTCGT GTCTCCTCGC TCCCCCCGCC 
CCATCCCGCA GCAAATCCCA AGAGACCCTC CGTTGCTCCC CTAATCCATT CCTCACAGAC 
ATCCAACCAA GACCCAACAG CACCAACCCC TTCACCAGCG GGCTGCAGTC CTCCCCGCGC 
CGATCGCTTA CACCTGATTT CTACACCCAA ATGCAAGCCT CTAAACCTGA TTTCAATAGA 
GCTATGTCTG CTGTAGGCCA CAGTTCCACC CTTCCTCCAA CGTTCTCCCG ACAACAGTCC 
TTAGTTACTG CTACAGCTCC AGCCCCCAAC AAACAAACGC AAAAGTGGGT CACCTTTGAT 
GATGATTTGG ATTTCCTTTC AAAAAGGGTT GGGACCGGCC CATTAGCTTC ACCTCTTCCT 
TTTCCACAAA CCCAAAGCAG TTTCCCAAGC TCAGGCTTTG GCATGGACAA CAACTGGGCA 
TCGCTTCCCA CCTCTGCATT CCCAGCAATC CCTCCGCCTG TACCAACCCG GACTAATACC 
AGCATCAAAA ACGCCCAACT CCCTTCCAGA AATGATTTCA CAGAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155418.1
CDS	118..4908
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_005155361.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155361.3

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGACAA AAAAGCCCTG CGTTAGCACG AGCAGATGCA 
GCTGGTCGAG GTCAAGCCAC CGGACCAGCT CCTGGAGGCA TCCCAAGGCC GATCCCACCC 
CGAGCAGGAG TCATCAGCGT CACGCCTCAG GCCAGACCTC CTCCTCCTGC TCATCCCGGG 
GCCCCCAGAC CCACAGCAGA GGTGCATCCT GGAGCCCCTC GACCCTCACC AGATAATCAT 
CCCGGAGCTC CAAGACCCAC TGCTGAACCC CAAAGCAAAC CGTCTGAGCT GCCTCTGGGT 
CCACCCCTGA CACTGCCAGG TCCCGTAAGG CCTCAGATGA CATCACCCAT GCAGCCTCAG 
TCTGTGTCGC CCGTTCAGCC TCCAGTCCAA CCCCAACTGC CCCCACCCAT ACAATCCCAG 
CTCCCTCCAC CAATGCAGCC CACCTTACCT GCTCCTCTGA TGCCTCAGCA GGCCCCTCAG 
ACATCAGCCG GAGCCGGAGC CGCTGCCGCC CCTCAGCCTG GACTGGCATC TCCCAAACCT 
CCACCCCGCA GCCGTTCCTC TCATGCACTG CCACCTGAAT CTGCTCCAGC TCCAACAACA 
CAGGCCAAGA ACATGAATGG AGTCCAAAGA GAAGCACAAT GGAACCTAGA CCCCTTCGAC 
ATGCTTAACC CTCAATCCCT CTTCCAGAAC ACACCATTCA CCGCTTCCCT CTCTCGCTCC 
TCATCCTCCT CTACATCCAC TCCATCCCCT TCTTCCTCAT CCTCAACGTT ACCCAGCAAC 
CTTTCCCTGT TCTCAGCTCC GGACACCAGC AGCGCTTCGT GTCTCCTCGC TCCCCCCGCC 
CCATCCCGCA GCAAATCCCA AGAGACCCTC CGTTGCTCCC CTAATCCATT CCTCACAGAC 
ATCCAACCAA GACCCAACAG CACCAACCCC TTCACCAGCG GGCTGCAGTC CTCCCCGCGC 
CGATCGCTTA CACCTGATTT CTACACCCAA ATGCAAGCCT CTAAACCTGA TTTCAATAGA 
GCTATGTCTG CTGTAGGCCA CAGTTCCACC CTTCCTCCAA CGTTCTCCCG ACAACAGTCC 
TTAGTTACTG CTACAGCTCC AGCCCCCAAC AAACAAACGC AAAAGTGGGT CACCTTTGAT 
GATGATTTGG ATTTCCTTTC AAAAAGGGTT GGGACCGGCC CATTAGCTTC ACCTCTTCCT 
TTTCCACAAA CCCAAAGCAG TTTCCCAAGC TCAGGCTTTG GCATGGACAA CAACTGGGCA 
TCGCTTCCCA CCTCTGCATT CCCAGCAATC CCTCCGCCTG TACCAACCCG GACTAATACC 
AGCATCAAAA ACGCCCAACT CCCTTCCAGA AATGATTTCA CAGAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155361.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155418.1 Danio rerio synaptojanin 1 (synj1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa16151
Clone ID Related Accession (Same CDS sequence)	XM_005155361.3 , XM_005155361.4
Accession Version	XM_005155361.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptojanin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155361.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGACAA AAAAGCCCTG CGTTAGCACG AGCAGATGCA 
GCTGGTCGAG GTCAAGCCAC CGGACCAGCT CCTGGAGGCA TCCCAAGGCC GATCCCACCC 
CGAGCAGGAG TCATCAGCGT CACGCCTCAG GCCAGACCTC CTCCTCCTGC TCATCCCGGG 
GCCCCCAGAC CCACAGCAGA GGTGCATCCT GGAGCCCCTC GACCCTCACC AGATAATCAT 
CCCGGAGCTC CAAGACCCAC TGCTGAACCC CAAAGCAAAC CGTCTGAGCT GCCTCTGGGT 
CCACCCCTGA CACTGCCAGG TCCCGTAAGG CCTCAGATGA CATCACCCAT GCAGCCTCAG 
TCTGTGTCGC CCGTTCAGCC TCCAGTCCAA CCCCAACTGC CCCCACCCAT ACAATCCCAG 
CTCCCTCCAC CAATGCAGCC CACCTTACCT GCTCCTCTGA TGCCTCAGCA GGCCCCTCAG 
ACATCAGCCG GAGCCGGAGC CGCTGCCGCC CCTCAGCCTG GACTGGCATC TCCCAAACCT 
CCACCCCGCA GCCGTTCCTC TCATGCACTG CCACCTGAAT CTGCTCCAGC TCCAACAACA 
CAGGCCAAGA ACATGAATGG AGTCCAAAGA GAAGCACAAT GGAACCTAGA CCCCTTCGAC 
ATGCTTAACC CTCAATCCCT CTTCCAGAAC ACACCATTCA CCGCTTCCCT CTCTCGCTCC 
TCATCCTCCT CTACATCCAC TCCATCCCCT TCTTCCTCAT CCTCAACGTT ACCCAGCAAC 
CTTTCCCTGT TCTCAGCTCC GGACACCAGC AGCGCTTCGT GTCTCCTCGC TCCCCCCGCC 
CCATCCCGCA GCAAATCCCA AGAGACCCTC CGTTGCTCCC CTAATCCATT CCTCACAGAC 
ATCCAACCAA GACCCAACAG CACCAACCCC TTCACCAGCG GGCTGCAGTC CTCCCCGCGC 
CGATCGCTTA CACCTGATTT CTACACCCAA ATGCAAGCCT CTAAACCTGA TTTCAATAGA 
GCTATGTCTG CTGTAGGCCA CAGTTCCACC CTTCCTCCAA CGTTCTCCCG ACAACAGTCC 
TTAGTTACTG CTACAGCTCC AGCCCCCAAC AAACAAACGC AAAAGTGGGT CACCTTTGAT 
GATGATTTGG ATTTCCTTTC AAAAAGGGTT GGGACCGGCC CATTAGCTTC ACCTCTTCCT 
TTTCCACAAA CCCAAAGCAG TTTCCCAAGC TCAGGCTTTG GCATGGACAA CAACTGGGCA 
TCGCTTCCCA CCTCTGCATT CCCAGCAATC CCTCCGCCTG TACCAACCCG GACTAATACC 
AGCATCAAAA ACGCCCAACT CCCTTCCAGA AATGATTTCA CAGAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155418.1
CDS	120..4910
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_005155361.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155361.4

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGACAA AAAAGCCCTG CGTTAGCACG AGCAGATGCA 
GCTGGTCGAG GTCAAGCCAC CGGACCAGCT CCTGGAGGCA TCCCAAGGCC GATCCCACCC 
CGAGCAGGAG TCATCAGCGT CACGCCTCAG GCCAGACCTC CTCCTCCTGC TCATCCCGGG 
GCCCCCAGAC CCACAGCAGA GGTGCATCCT GGAGCCCCTC GACCCTCACC AGATAATCAT 
CCCGGAGCTC CAAGACCCAC TGCTGAACCC CAAAGCAAAC CGTCTGAGCT GCCTCTGGGT 
CCACCCCTGA CACTGCCAGG TCCCGTAAGG CCTCAGATGA CATCACCCAT GCAGCCTCAG 
TCTGTGTCGC CCGTTCAGCC TCCAGTCCAA CCCCAACTGC CCCCACCCAT ACAATCCCAG 
CTCCCTCCAC CAATGCAGCC CACCTTACCT GCTCCTCTGA TGCCTCAGCA GGCCCCTCAG 
ACATCAGCCG GAGCCGGAGC CGCTGCCGCC CCTCAGCCTG GACTGGCATC TCCCAAACCT 
CCACCCCGCA GCCGTTCCTC TCATGCACTG CCACCTGAAT CTGCTCCAGC TCCAACAACA 
CAGGCCAAGA ACATGAATGG AGTCCAAAGA GAAGCACAAT GGAACCTAGA CCCCTTCGAC 
ATGCTTAACC CTCAATCCCT CTTCCAGAAC ACACCATTCA CCGCTTCCCT CTCTCGCTCC 
TCATCCTCCT CTACATCCAC TCCATCCCCT TCTTCCTCAT CCTCAACGTT ACCCAGCAAC 
CTTTCCCTGT TCTCAGCTCC GGACACCAGC AGCGCTTCGT GTCTCCTCGC TCCCCCCGCC 
CCATCCCGCA GCAAATCCCA AGAGACCCTC CGTTGCTCCC CTAATCCATT CCTCACAGAC 
ATCCAACCAA GACCCAACAG CACCAACCCC TTCACCAGCG GGCTGCAGTC CTCCCCGCGC 
CGATCGCTTA CACCTGATTT CTACACCCAA ATGCAAGCCT CTAAACCTGA TTTCAATAGA 
GCTATGTCTG CTGTAGGCCA CAGTTCCACC CTTCCTCCAA CGTTCTCCCG ACAACAGTCC 
TTAGTTACTG CTACAGCTCC AGCCCCCAAC AAACAAACGC AAAAGTGGGT CACCTTTGAT 
GATGATTTGG ATTTCCTTTC AAAAAGGGTT GGGACCGGCC CATTAGCTTC ACCTCTTCCT 
TTTCCACAAA CCCAAAGCAG TTTCCCAAGC TCAGGCTTTG GCATGGACAA CAACTGGGCA 
TCGCTTCCCA CCTCTGCATT CCCAGCAATC CCTCCGCCTG TACCAACCCG GACTAATACC 
AGCATCAAAA ACGCCCAACT CCCTTCCAGA AATGATTTCA CAGAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155362.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155419.1 Danio rerio synaptojanin 1 (synj1), transcript variant X7, mRNA.

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CloneID	ODa16152
Clone ID Related Accession (Same CDS sequence)	XM_005155362.3 , XM_005155362.4
Accession Version	XM_005155362.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synaptojanin-1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155362.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGACA AAAAAGCCCT 
GCGTTAGCAC GAGCAGATGC AGCTGGTCGA GGTCAAGCCA CCGGACCAGC TCCTGGAGGC 
ATCCCAAGGC CGATCCCACC CCGAGCAGGA GTCATCAGCG TCACGCCTCA GGCCAGACCT 
CCTCCTCCTG CTCATCCCGG GGCCCCCAGA CCCACAGCAG AGGTGCATCC TGGAGCCCCT 
CGACCCTCAC CAGATAATCA TCCCGGAGCT CCAAGACCCA CTGCTGAACC CCAAAGCAAA 
CCGTCTGAGC TGCCTCTGGG TCCACCCCTG ACACTGCCAG GTCCCGTAAG GCCTCAGATG 
ACATCACCCA TGCAGCCTCA GTCTGTGTCG CCCGTTCAGC CTCCAGTCCA ACCCCAACTG 
CCCCCACCCA TACAATCCCA GCTCCCTCCA CCAATGCAGC CCACCTTACC TGCTCCTCTG 
ATGCCTCAGC AGGCCCCTCA GACATCAGCC GGAGCCGGAG CCGCTGCCGC CCCTCAGCCT 
GGACTGGCAT CTCCCAAACC TCCACCCCGC AGCCGTTCCT CTCATGCACT GCCACCTGAA 
TCTGCTCCAG CTCCAACAAC ACAGGCCAAG AACATGAATG GAGTCCAAAG AGAAGCACAA 
TGGAACCTAG ACCCCTTCGA CATGCTTAAC CCTCAATCCC TCTTCCAGAA CACACCATTC 
ACCGCTTCCC TCTCTCGCTC CTCATCCTCC TCTACATCCA CTCCATCCCC TTCTTCCTCA 
TCCTCAACGT TACCCAGCAA CCTTTCCCTG TTCTCAGCTC CGGACACCAG CAGCGCTTCG 
TGTCTCCTCG CTCCCCCCGC CCCATCCCGC AGCAAATCCC AAGAGACCCT CCGTTGCTCC 
CCTAATCCAT TCCTCACAGA CATCCAACCA AGACCCAACA GCACCAACCC CTTCACCAGC 
GGGCTGCAGT CCTCCCCGCG CCGATCGCTT ACACCTGATT TCTACACCCA AATGCAAGCC 
TCTAAACCTG ATTTCAATAG AGCTATGTCT GCTGTAGGCC ACAGTTCCAC CCTTCCTCCA 
ACGTTCTCCC GACAACAGTC CTTAGTTACT GCTACAGCTC CAGCCCCCAA CAAACAAACG 
CAAAAGTGGG TCACCTTTGA TGATGATTTG GATTTCCTTT CAAAAAGGGT TGGGACCGGC 
CCATTAGCTT CACCTCTTCC TTTTCCACAA ACCCAAAGCA GTTTCCCAAG CTCAGGCTTT 
GGCATGGACA ACAACTGGGC ATCGCTTCCC ACCTCTGCAT TCCCAGCAAT CCCTCCGCCT 
GTACCAACCC GGACTAATAC CAGCATCAAA AACGCCCAAC TCCCTTCCAG AAATGATTTC 
ACAGAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155419.1
CDS	118..4869
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_005155362.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155362.3

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGACA AAAAAGCCCT 
GCGTTAGCAC GAGCAGATGC AGCTGGTCGA GGTCAAGCCA CCGGACCAGC TCCTGGAGGC 
ATCCCAAGGC CGATCCCACC CCGAGCAGGA GTCATCAGCG TCACGCCTCA GGCCAGACCT 
CCTCCTCCTG CTCATCCCGG GGCCCCCAGA CCCACAGCAG AGGTGCATCC TGGAGCCCCT 
CGACCCTCAC CAGATAATCA TCCCGGAGCT CCAAGACCCA CTGCTGAACC CCAAAGCAAA 
CCGTCTGAGC TGCCTCTGGG TCCACCCCTG ACACTGCCAG GTCCCGTAAG GCCTCAGATG 
ACATCACCCA TGCAGCCTCA GTCTGTGTCG CCCGTTCAGC CTCCAGTCCA ACCCCAACTG 
CCCCCACCCA TACAATCCCA GCTCCCTCCA CCAATGCAGC CCACCTTACC TGCTCCTCTG 
ATGCCTCAGC AGGCCCCTCA GACATCAGCC GGAGCCGGAG CCGCTGCCGC CCCTCAGCCT 
GGACTGGCAT CTCCCAAACC TCCACCCCGC AGCCGTTCCT CTCATGCACT GCCACCTGAA 
TCTGCTCCAG CTCCAACAAC ACAGGCCAAG AACATGAATG GAGTCCAAAG AGAAGCACAA 
TGGAACCTAG ACCCCTTCGA CATGCTTAAC CCTCAATCCC TCTTCCAGAA CACACCATTC 
ACCGCTTCCC TCTCTCGCTC CTCATCCTCC TCTACATCCA CTCCATCCCC TTCTTCCTCA 
TCCTCAACGT TACCCAGCAA CCTTTCCCTG TTCTCAGCTC CGGACACCAG CAGCGCTTCG 
TGTCTCCTCG CTCCCCCCGC CCCATCCCGC AGCAAATCCC AAGAGACCCT CCGTTGCTCC 
CCTAATCCAT TCCTCACAGA CATCCAACCA AGACCCAACA GCACCAACCC CTTCACCAGC 
GGGCTGCAGT CCTCCCCGCG CCGATCGCTT ACACCTGATT TCTACACCCA AATGCAAGCC 
TCTAAACCTG ATTTCAATAG AGCTATGTCT GCTGTAGGCC ACAGTTCCAC CCTTCCTCCA 
ACGTTCTCCC GACAACAGTC CTTAGTTACT GCTACAGCTC CAGCCCCCAA CAAACAAACG 
CAAAAGTGGG TCACCTTTGA TGATGATTTG GATTTCCTTT CAAAAAGGGT TGGGACCGGC 
CCATTAGCTT CACCTCTTCC TTTTCCACAA ACCCAAAGCA GTTTCCCAAG CTCAGGCTTT 
GGCATGGACA ACAACTGGGC ATCGCTTCCC ACCTCTGCAT TCCCAGCAAT CCCTCCGCCT 
GTACCAACCC GGACTAATAC CAGCATCAAA AACGCCCAAC TCCCTTCCAG AAATGATTTC 
ACAGAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155362.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155419.1 Danio rerio synaptojanin 1 (synj1), transcript variant X7, mRNA.

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CloneID	ODa16152
Clone ID Related Accession (Same CDS sequence)	XM_005155362.3 , XM_005155362.4
Accession Version	XM_005155362.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptojanin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155362.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGACA AAAAAGCCCT 
GCGTTAGCAC GAGCAGATGC AGCTGGTCGA GGTCAAGCCA CCGGACCAGC TCCTGGAGGC 
ATCCCAAGGC CGATCCCACC CCGAGCAGGA GTCATCAGCG TCACGCCTCA GGCCAGACCT 
CCTCCTCCTG CTCATCCCGG GGCCCCCAGA CCCACAGCAG AGGTGCATCC TGGAGCCCCT 
CGACCCTCAC CAGATAATCA TCCCGGAGCT CCAAGACCCA CTGCTGAACC CCAAAGCAAA 
CCGTCTGAGC TGCCTCTGGG TCCACCCCTG ACACTGCCAG GTCCCGTAAG GCCTCAGATG 
ACATCACCCA TGCAGCCTCA GTCTGTGTCG CCCGTTCAGC CTCCAGTCCA ACCCCAACTG 
CCCCCACCCA TACAATCCCA GCTCCCTCCA CCAATGCAGC CCACCTTACC TGCTCCTCTG 
ATGCCTCAGC AGGCCCCTCA GACATCAGCC GGAGCCGGAG CCGCTGCCGC CCCTCAGCCT 
GGACTGGCAT CTCCCAAACC TCCACCCCGC AGCCGTTCCT CTCATGCACT GCCACCTGAA 
TCTGCTCCAG CTCCAACAAC ACAGGCCAAG AACATGAATG GAGTCCAAAG AGAAGCACAA 
TGGAACCTAG ACCCCTTCGA CATGCTTAAC CCTCAATCCC TCTTCCAGAA CACACCATTC 
ACCGCTTCCC TCTCTCGCTC CTCATCCTCC TCTACATCCA CTCCATCCCC TTCTTCCTCA 
TCCTCAACGT TACCCAGCAA CCTTTCCCTG TTCTCAGCTC CGGACACCAG CAGCGCTTCG 
TGTCTCCTCG CTCCCCCCGC CCCATCCCGC AGCAAATCCC AAGAGACCCT CCGTTGCTCC 
CCTAATCCAT TCCTCACAGA CATCCAACCA AGACCCAACA GCACCAACCC CTTCACCAGC 
GGGCTGCAGT CCTCCCCGCG CCGATCGCTT ACACCTGATT TCTACACCCA AATGCAAGCC 
TCTAAACCTG ATTTCAATAG AGCTATGTCT GCTGTAGGCC ACAGTTCCAC CCTTCCTCCA 
ACGTTCTCCC GACAACAGTC CTTAGTTACT GCTACAGCTC CAGCCCCCAA CAAACAAACG 
CAAAAGTGGG TCACCTTTGA TGATGATTTG GATTTCCTTT CAAAAAGGGT TGGGACCGGC 
CCATTAGCTT CACCTCTTCC TTTTCCACAA ACCCAAAGCA GTTTCCCAAG CTCAGGCTTT 
GGCATGGACA ACAACTGGGC ATCGCTTCCC ACCTCTGCAT TCCCAGCAAT CCCTCCGCCT 
GTACCAACCC GGACTAATAC CAGCATCAAA AACGCCCAAC TCCCTTCCAG AAATGATTTC 
ACAGAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155419.1
CDS	120..4871
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_005155362.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155362.4

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGACA AAAAAGCCCT 
GCGTTAGCAC GAGCAGATGC AGCTGGTCGA GGTCAAGCCA CCGGACCAGC TCCTGGAGGC 
ATCCCAAGGC CGATCCCACC CCGAGCAGGA GTCATCAGCG TCACGCCTCA GGCCAGACCT 
CCTCCTCCTG CTCATCCCGG GGCCCCCAGA CCCACAGCAG AGGTGCATCC TGGAGCCCCT 
CGACCCTCAC CAGATAATCA TCCCGGAGCT CCAAGACCCA CTGCTGAACC CCAAAGCAAA 
CCGTCTGAGC TGCCTCTGGG TCCACCCCTG ACACTGCCAG GTCCCGTAAG GCCTCAGATG 
ACATCACCCA TGCAGCCTCA GTCTGTGTCG CCCGTTCAGC CTCCAGTCCA ACCCCAACTG 
CCCCCACCCA TACAATCCCA GCTCCCTCCA CCAATGCAGC CCACCTTACC TGCTCCTCTG 
ATGCCTCAGC AGGCCCCTCA GACATCAGCC GGAGCCGGAG CCGCTGCCGC CCCTCAGCCT 
GGACTGGCAT CTCCCAAACC TCCACCCCGC AGCCGTTCCT CTCATGCACT GCCACCTGAA 
TCTGCTCCAG CTCCAACAAC ACAGGCCAAG AACATGAATG GAGTCCAAAG AGAAGCACAA 
TGGAACCTAG ACCCCTTCGA CATGCTTAAC CCTCAATCCC TCTTCCAGAA CACACCATTC 
ACCGCTTCCC TCTCTCGCTC CTCATCCTCC TCTACATCCA CTCCATCCCC TTCTTCCTCA 
TCCTCAACGT TACCCAGCAA CCTTTCCCTG TTCTCAGCTC CGGACACCAG CAGCGCTTCG 
TGTCTCCTCG CTCCCCCCGC CCCATCCCGC AGCAAATCCC AAGAGACCCT CCGTTGCTCC 
CCTAATCCAT TCCTCACAGA CATCCAACCA AGACCCAACA GCACCAACCC CTTCACCAGC 
GGGCTGCAGT CCTCCCCGCG CCGATCGCTT ACACCTGATT TCTACACCCA AATGCAAGCC 
TCTAAACCTG ATTTCAATAG AGCTATGTCT GCTGTAGGCC ACAGTTCCAC CCTTCCTCCA 
ACGTTCTCCC GACAACAGTC CTTAGTTACT GCTACAGCTC CAGCCCCCAA CAAACAAACG 
CAAAAGTGGG TCACCTTTGA TGATGATTTG GATTTCCTTT CAAAAAGGGT TGGGACCGGC 
CCATTAGCTT CACCTCTTCC TTTTCCACAA ACCCAAAGCA GTTTCCCAAG CTCAGGCTTT 
GGCATGGACA ACAACTGGGC ATCGCTTCCC ACCTCTGCAT TCCCAGCAAT CCCTCCGCCT 
GTACCAACCC GGACTAATAC CAGCATCAAA AACGCCCAAC TCCCTTCCAG AAATGATTTC 
ACAGAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155363.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155420.1 Danio rerio synaptojanin 1 (synj1), transcript variant X9, mRNA.

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CloneID	ODa16153
Clone ID Related Accession (Same CDS sequence)	XM_005155363.3 , XM_005155363.4
Accession Version	XM_005155363.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synaptojanin-1 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155363.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTCG AGGTCAAGCC 
ACCGGACCAG CTCCTGGAGG CATCCCAAGG CCGATCCCAC CCCGAGCAGG AGTCATCAGC 
GTCACGCCTC AGGCCAGACC TCCTCCTCCT GCTCATCCCG GGGCCCCCAG ACCCACAGCA 
GAGGTGCATC CTGGAGCCCC TCGACCCTCA CCAGATAATC ATCCCGGAGC TCCAAGACCC 
ACTGCTGAAC CCCAAAGCAA ACCGTCTGAG CTGCCTCTGG GTCCACCCCT GACACTGCCA 
GGTCCCGTAA GGCCTCAGAT GACATCACCC ATGCAGCCTC AGTCTGTGTC GCCCGTTCAG 
CCTCCAGTCC AACCCCAACT GCCCCCACCC ATACAATCCC AGCTCCCTCC ACCAATGCAG 
CCCACCTTAC CTGCTCCTCT GATGCCTCAG CAGGCCCCTC AGACATCAGC CGGAGCCGGA 
GCCGCTGCCG CCCCTCAGCC TGGACTGGCA TCTCCCAAAC CTCCACCCCG CAGCCGTTCC 
TCTCATGCAC TGCCACCTGA ATCTGCTCCA GCTCCAACAA CACAGGCCAA GAACATGAAT 
GGAGTCCAAA GAGAAGCACA ATGGAACCTA GACCCCTTCG ACATGCTTAA CCCTCAATCC 
CTCTTCCAGA ACACACCATT CACCGCTTCC CTCTCTCGCT CCTCATCCTC CTCTACATCC 
ACTCCATCCC CTTCTTCCTC ATCCTCAACG TTACCCAGCA ACCTTTCCCT GTTCTCAGCT 
CCGGACACCA GCAGCGCTTC GTGTCTCCTC GCTCCCCCCG CCCCATCCCG CAGCAAATCC 
CAAGAGACCC TCCGTTGCTC CCCTAATCCA TTCCTCACAG ACATCCAACC AAGACCCAAC 
AGCACCAACC CCTTCACCAG CGGGCTGCAG TCCTCCCCGC GCCGATCGCT TACACCTGAT 
TTCTACACCC AAATGCAAGC CTCTAAACCT GATTTCAATA GAGCTATGTC TGCTGTAGGC 
CACAGTTCCA CCCTTCCTCC AACGTTCTCC CGACAACAGT CCTTAGTTAC TGCTACAGCT 
CCAGCCCCCA ACAAACAAAC GCAAAAGTGG GTCACCTTTG ATGATGATTT GGATTTCCTT 
TCAAAAAGGG TTGGGACCGG CCCATTAGCT TCACCTCTTC CTTTTCCACA AACCCAAAGC 
AGTTTCCCAA GCTCAGGCTT TGGCATGGAC AACAACTGGG CATCGCTTCC CACCTCTGCA 
TTCCCAGCAA TCCCTCCGCC TGTACCAACC CGGACTAATA CCAGCATCAA AAACGCCCAA 
CTCCCTTCCA GAAATGATTT CACAGAGAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155420.1
CDS	118..4830
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_005155363.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155363.3

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTCG AGGTCAAGCC 
ACCGGACCAG CTCCTGGAGG CATCCCAAGG CCGATCCCAC CCCGAGCAGG AGTCATCAGC 
GTCACGCCTC AGGCCAGACC TCCTCCTCCT GCTCATCCCG GGGCCCCCAG ACCCACAGCA 
GAGGTGCATC CTGGAGCCCC TCGACCCTCA CCAGATAATC ATCCCGGAGC TCCAAGACCC 
ACTGCTGAAC CCCAAAGCAA ACCGTCTGAG CTGCCTCTGG GTCCACCCCT GACACTGCCA 
GGTCCCGTAA GGCCTCAGAT GACATCACCC ATGCAGCCTC AGTCTGTGTC GCCCGTTCAG 
CCTCCAGTCC AACCCCAACT GCCCCCACCC ATACAATCCC AGCTCCCTCC ACCAATGCAG 
CCCACCTTAC CTGCTCCTCT GATGCCTCAG CAGGCCCCTC AGACATCAGC CGGAGCCGGA 
GCCGCTGCCG CCCCTCAGCC TGGACTGGCA TCTCCCAAAC CTCCACCCCG CAGCCGTTCC 
TCTCATGCAC TGCCACCTGA ATCTGCTCCA GCTCCAACAA CACAGGCCAA GAACATGAAT 
GGAGTCCAAA GAGAAGCACA ATGGAACCTA GACCCCTTCG ACATGCTTAA CCCTCAATCC 
CTCTTCCAGA ACACACCATT CACCGCTTCC CTCTCTCGCT CCTCATCCTC CTCTACATCC 
ACTCCATCCC CTTCTTCCTC ATCCTCAACG TTACCCAGCA ACCTTTCCCT GTTCTCAGCT 
CCGGACACCA GCAGCGCTTC GTGTCTCCTC GCTCCCCCCG CCCCATCCCG CAGCAAATCC 
CAAGAGACCC TCCGTTGCTC CCCTAATCCA TTCCTCACAG ACATCCAACC AAGACCCAAC 
AGCACCAACC CCTTCACCAG CGGGCTGCAG TCCTCCCCGC GCCGATCGCT TACACCTGAT 
TTCTACACCC AAATGCAAGC CTCTAAACCT GATTTCAATA GAGCTATGTC TGCTGTAGGC 
CACAGTTCCA CCCTTCCTCC AACGTTCTCC CGACAACAGT CCTTAGTTAC TGCTACAGCT 
CCAGCCCCCA ACAAACAAAC GCAAAAGTGG GTCACCTTTG ATGATGATTT GGATTTCCTT 
TCAAAAAGGG TTGGGACCGG CCCATTAGCT TCACCTCTTC CTTTTCCACA AACCCAAAGC 
AGTTTCCCAA GCTCAGGCTT TGGCATGGAC AACAACTGGG CATCGCTTCC CACCTCTGCA 
TTCCCAGCAA TCCCTCCGCC TGTACCAACC CGGACTAATA CCAGCATCAA AAACGCCCAA 
CTCCCTTCCA GAAATGATTT CACAGAGAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155363.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155420.1 Danio rerio synaptojanin 1 (synj1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa16153
Clone ID Related Accession (Same CDS sequence)	XM_005155363.3 , XM_005155363.4
Accession Version	XM_005155363.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptojanin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155363.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTCG AGGTCAAGCC 
ACCGGACCAG CTCCTGGAGG CATCCCAAGG CCGATCCCAC CCCGAGCAGG AGTCATCAGC 
GTCACGCCTC AGGCCAGACC TCCTCCTCCT GCTCATCCCG GGGCCCCCAG ACCCACAGCA 
GAGGTGCATC CTGGAGCCCC TCGACCCTCA CCAGATAATC ATCCCGGAGC TCCAAGACCC 
ACTGCTGAAC CCCAAAGCAA ACCGTCTGAG CTGCCTCTGG GTCCACCCCT GACACTGCCA 
GGTCCCGTAA GGCCTCAGAT GACATCACCC ATGCAGCCTC AGTCTGTGTC GCCCGTTCAG 
CCTCCAGTCC AACCCCAACT GCCCCCACCC ATACAATCCC AGCTCCCTCC ACCAATGCAG 
CCCACCTTAC CTGCTCCTCT GATGCCTCAG CAGGCCCCTC AGACATCAGC CGGAGCCGGA 
GCCGCTGCCG CCCCTCAGCC TGGACTGGCA TCTCCCAAAC CTCCACCCCG CAGCCGTTCC 
TCTCATGCAC TGCCACCTGA ATCTGCTCCA GCTCCAACAA CACAGGCCAA GAACATGAAT 
GGAGTCCAAA GAGAAGCACA ATGGAACCTA GACCCCTTCG ACATGCTTAA CCCTCAATCC 
CTCTTCCAGA ACACACCATT CACCGCTTCC CTCTCTCGCT CCTCATCCTC CTCTACATCC 
ACTCCATCCC CTTCTTCCTC ATCCTCAACG TTACCCAGCA ACCTTTCCCT GTTCTCAGCT 
CCGGACACCA GCAGCGCTTC GTGTCTCCTC GCTCCCCCCG CCCCATCCCG CAGCAAATCC 
CAAGAGACCC TCCGTTGCTC CCCTAATCCA TTCCTCACAG ACATCCAACC AAGACCCAAC 
AGCACCAACC CCTTCACCAG CGGGCTGCAG TCCTCCCCGC GCCGATCGCT TACACCTGAT 
TTCTACACCC AAATGCAAGC CTCTAAACCT GATTTCAATA GAGCTATGTC TGCTGTAGGC 
CACAGTTCCA CCCTTCCTCC AACGTTCTCC CGACAACAGT CCTTAGTTAC TGCTACAGCT 
CCAGCCCCCA ACAAACAAAC GCAAAAGTGG GTCACCTTTG ATGATGATTT GGATTTCCTT 
TCAAAAAGGG TTGGGACCGG CCCATTAGCT TCACCTCTTC CTTTTCCACA AACCCAAAGC 
AGTTTCCCAA GCTCAGGCTT TGGCATGGAC AACAACTGGG CATCGCTTCC CACCTCTGCA 
TTCCCAGCAA TCCCTCCGCC TGTACCAACC CGGACTAATA CCAGCATCAA AAACGCCCAA 
CTCCCTTCCA GAAATGATTT CACAGAGAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155420.1
CDS	120..4832
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_005155363.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155363.4

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTCG AGGTCAAGCC 
ACCGGACCAG CTCCTGGAGG CATCCCAAGG CCGATCCCAC CCCGAGCAGG AGTCATCAGC 
GTCACGCCTC AGGCCAGACC TCCTCCTCCT GCTCATCCCG GGGCCCCCAG ACCCACAGCA 
GAGGTGCATC CTGGAGCCCC TCGACCCTCA CCAGATAATC ATCCCGGAGC TCCAAGACCC 
ACTGCTGAAC CCCAAAGCAA ACCGTCTGAG CTGCCTCTGG GTCCACCCCT GACACTGCCA 
GGTCCCGTAA GGCCTCAGAT GACATCACCC ATGCAGCCTC AGTCTGTGTC GCCCGTTCAG 
CCTCCAGTCC AACCCCAACT GCCCCCACCC ATACAATCCC AGCTCCCTCC ACCAATGCAG 
CCCACCTTAC CTGCTCCTCT GATGCCTCAG CAGGCCCCTC AGACATCAGC CGGAGCCGGA 
GCCGCTGCCG CCCCTCAGCC TGGACTGGCA TCTCCCAAAC CTCCACCCCG CAGCCGTTCC 
TCTCATGCAC TGCCACCTGA ATCTGCTCCA GCTCCAACAA CACAGGCCAA GAACATGAAT 
GGAGTCCAAA GAGAAGCACA ATGGAACCTA GACCCCTTCG ACATGCTTAA CCCTCAATCC 
CTCTTCCAGA ACACACCATT CACCGCTTCC CTCTCTCGCT CCTCATCCTC CTCTACATCC 
ACTCCATCCC CTTCTTCCTC ATCCTCAACG TTACCCAGCA ACCTTTCCCT GTTCTCAGCT 
CCGGACACCA GCAGCGCTTC GTGTCTCCTC GCTCCCCCCG CCCCATCCCG CAGCAAATCC 
CAAGAGACCC TCCGTTGCTC CCCTAATCCA TTCCTCACAG ACATCCAACC AAGACCCAAC 
AGCACCAACC CCTTCACCAG CGGGCTGCAG TCCTCCCCGC GCCGATCGCT TACACCTGAT 
TTCTACACCC AAATGCAAGC CTCTAAACCT GATTTCAATA GAGCTATGTC TGCTGTAGGC 
CACAGTTCCA CCCTTCCTCC AACGTTCTCC CGACAACAGT CCTTAGTTAC TGCTACAGCT 
CCAGCCCCCA ACAAACAAAC GCAAAAGTGG GTCACCTTTG ATGATGATTT GGATTTCCTT 
TCAAAAAGGG TTGGGACCGG CCCATTAGCT TCACCTCTTC CTTTTCCACA AACCCAAAGC 
AGTTTCCCAA GCTCAGGCTT TGGCATGGAC AACAACTGGG CATCGCTTCC CACCTCTGCA 
TTCCCAGCAA TCCCTCCGCC TGTACCAACC CGGACTAATA CCAGCATCAA AAACGCCCAA 
CTCCCTTCCA GAAATGATTT CACAGAGAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155364.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155421.1 Danio rerio synaptojanin 1 (synj1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa16154
Clone ID Related Accession (Same CDS sequence)	XM_005155364.4 , XM_005155364.3
Accession Version	XM_005155364.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptojanin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155364.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGCTTC GCTGAGCCTC TCAATCTAGA GTTTGATCTG 
ACTGATAATG AAGGACAAAA AAGCCCTGCG TTAGCACGAG CAGATGCAGC TGGTCGAGGT 
CAAGCCACCG GACCAGCTCC TGGAGGCATC CCAAGGCCGA TCCCACCCCG AGCAGGAGTC 
ATCAGCGTCA CGCCTCAGGC CAGACCTCCT CCTCCTGCTC ATCCCGGGGC CCCCAGACCC 
ACAGCAGAGG TGCATCCTGG AGCCCCTCGA CCCTCACCAG ATAATCATCC CGGAGCTCCA 
AGACCCACTG CTGAACCCCA AAGCAAACCG TCTGAGCTGC CTCTGGGTCC ACCCCTGACA 
CTGCCAGGTC CCGTAAGGCC TCAGATGACA TCACCCATGC AGCCTCAGTC TGTGTCGCCC 
GTTCAGCCTC CAGTCCAACC CCAACTGCCC CCACCCATAC AATCCCAGCT CCCTCCACCA 
ATGCAGCCCA CCTTACCTGC TCCTCTGATG CCTCAGCAGG CCCCTCAGAC ATCAGCCGGA 
GCCGGAGCCG CTGCCGCCCC TCAGCCTGGA CTGGCATCTC CCAAACCTCC ACCCCGCAGC 
CGTTCCTCTC ATGCACTGCC ACCTGAATCT GCTCCAGCTC CAACAACACA GCAGGAGCAA 
TCCTCAGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155421.1
CDS	120..4211
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQQEQSSG

Target ORF information:

RefSeq Version	XM_005155364.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155364.4

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGCTTC GCTGAGCCTC TCAATCTAGA GTTTGATCTG 
ACTGATAATG AAGGACAAAA AAGCCCTGCG TTAGCACGAG CAGATGCAGC TGGTCGAGGT 
CAAGCCACCG GACCAGCTCC TGGAGGCATC CCAAGGCCGA TCCCACCCCG AGCAGGAGTC 
ATCAGCGTCA CGCCTCAGGC CAGACCTCCT CCTCCTGCTC ATCCCGGGGC CCCCAGACCC 
ACAGCAGAGG TGCATCCTGG AGCCCCTCGA CCCTCACCAG ATAATCATCC CGGAGCTCCA 
AGACCCACTG CTGAACCCCA AAGCAAACCG TCTGAGCTGC CTCTGGGTCC ACCCCTGACA 
CTGCCAGGTC CCGTAAGGCC TCAGATGACA TCACCCATGC AGCCTCAGTC TGTGTCGCCC 
GTTCAGCCTC CAGTCCAACC CCAACTGCCC CCACCCATAC AATCCCAGCT CCCTCCACCA 
ATGCAGCCCA CCTTACCTGC TCCTCTGATG CCTCAGCAGG CCCCTCAGAC ATCAGCCGGA 
GCCGGAGCCG CTGCCGCCCC TCAGCCTGGA CTGGCATCTC CCAAACCTCC ACCCCGCAGC 
CGTTCCTCTC ATGCACTGCC ACCTGAATCT GCTCCAGCTC CAACAACACA GCAGGAGCAA 
TCCTCAGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_005155364.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155421.1 Danio rerio synaptojanin 1 (synj1), transcript variant X10, mRNA.

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CloneID	ODa16154
Clone ID Related Accession (Same CDS sequence)	XM_005155364.4 , XM_005155364.3
Accession Version	XM_005155364.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synaptojanin-1 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155364.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGCTTC GCTGAGCCTC TCAATCTAGA GTTTGATCTG 
ACTGATAATG AAGGACAAAA AAGCCCTGCG TTAGCACGAG CAGATGCAGC TGGTCGAGGT 
CAAGCCACCG GACCAGCTCC TGGAGGCATC CCAAGGCCGA TCCCACCCCG AGCAGGAGTC 
ATCAGCGTCA CGCCTCAGGC CAGACCTCCT CCTCCTGCTC ATCCCGGGGC CCCCAGACCC 
ACAGCAGAGG TGCATCCTGG AGCCCCTCGA CCCTCACCAG ATAATCATCC CGGAGCTCCA 
AGACCCACTG CTGAACCCCA AAGCAAACCG TCTGAGCTGC CTCTGGGTCC ACCCCTGACA 
CTGCCAGGTC CCGTAAGGCC TCAGATGACA TCACCCATGC AGCCTCAGTC TGTGTCGCCC 
GTTCAGCCTC CAGTCCAACC CCAACTGCCC CCACCCATAC AATCCCAGCT CCCTCCACCA 
ATGCAGCCCA CCTTACCTGC TCCTCTGATG CCTCAGCAGG CCCCTCAGAC ATCAGCCGGA 
GCCGGAGCCG CTGCCGCCCC TCAGCCTGGA CTGGCATCTC CCAAACCTCC ACCCCGCAGC 
CGTTCCTCTC ATGCACTGCC ACCTGAATCT GCTCCAGCTC CAACAACACA GCAGGAGCAA 
TCCTCAGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155421.1
CDS	118..4209
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQQEQSSG

Target ORF information:

RefSeq Version	XM_005155364.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155364.3

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGCTTC GCTGAGCCTC TCAATCTAGA GTTTGATCTG 
ACTGATAATG AAGGACAAAA AAGCCCTGCG TTAGCACGAG CAGATGCAGC TGGTCGAGGT 
CAAGCCACCG GACCAGCTCC TGGAGGCATC CCAAGGCCGA TCCCACCCCG AGCAGGAGTC 
ATCAGCGTCA CGCCTCAGGC CAGACCTCCT CCTCCTGCTC ATCCCGGGGC CCCCAGACCC 
ACAGCAGAGG TGCATCCTGG AGCCCCTCGA CCCTCACCAG ATAATCATCC CGGAGCTCCA 
AGACCCACTG CTGAACCCCA AAGCAAACCG TCTGAGCTGC CTCTGGGTCC ACCCCTGACA 
CTGCCAGGTC CCGTAAGGCC TCAGATGACA TCACCCATGC AGCCTCAGTC TGTGTCGCCC 
GTTCAGCCTC CAGTCCAACC CCAACTGCCC CCACCCATAC AATCCCAGCT CCCTCCACCA 
ATGCAGCCCA CCTTACCTGC TCCTCTGATG CCTCAGCAGG CCCCTCAGAC ATCAGCCGGA 
GCCGGAGCCG CTGCCGCCCC TCAGCCTGGA CTGGCATCTC CCAAACCTCC ACCCCGCAGC 
CGTTCCTCTC ATGCACTGCC ACCTGAATCT GCTCCAGCTC CAACAACACA GCAGGAGCAA 
TCCTCAGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_009305307.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303582.1 Danio rerio synaptojanin 1 (synj1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa40105
Clone ID Related Accession (Same CDS sequence)	XM_009305307.2 , XM_009305307.3
Accession Version	XM_009305307.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synaptojanin-1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305307.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGCCTCT CCAAGGGTGC TTCTAGGCAT GTGTCAGAAC 
CACTTCAAAT ACACTCGGCC CAAGAAGATC CGAGTATGTG TGGGAACGTG GAACGTAAAT 
GGAGGCAAAC AGTTTCGCAG CATTGCGTTT CGTAACCATA CGCTCAACGA CTGGCTTCTG 
GATGCTCCTA AGAAAGCCGG ACACCCAGAA TTTCAGGATG TCAAAAACAA CCCTGTGGAC 
ATCTTTGCTA TTGGTTTTGA GGAAATGGTG GAGCTGAATG CTGGTAATAT AGTCAGCGCA 
AGCACCACAA ACCAAAAGCT GTGGGCCGCA GAGCTGCAGA AGAACATCTC CCGAGATCAA 
CGATATGTCC TGCTGGCCTC AGAACAGCTG GTCGGTGTGT GTCTTTTCGT CTTCATTCGC 
CCTCAGCACG CACCCTTTAT CAGGGATGTT GCTGTAGACA CAGTGAAGAC TGGAATGGGC 
GGAGCCACTG GTAATAAGGG AGGCGTGGCT ATTCGTATGC TCTTCCACAC TACCAGCATC 
TGCTTCGTGT GTTCGCACTT TGCTGCCGGT CAATCACAAG TCAAAGAGAG GAACGACGAC 
TATAATGAGA TTGCACGCAA ACTGTCCTTC CCCATGGGTC GTCTTCTGTA CTCTCATGAT 
TATGTATTCT GGTGTGGAGA CTTCAACTAC CGAATCAATA TTCCCAATGA AGAGGTGAAG 
GAGCTGATCA GACAGCAGAA CTGGGATGCA CTTATTGGTG GAGATCAACT GGTGGAGCAG 
AAGAATGCAG GACAGGTCTT CAGAGGCTTT ATTGAAGGAA AGCTGGATTT TGCGCCCACT 
TATAAATACG ACCTTTTTTC TGAGGATTAC GACACTAGTG AGAAGTGCCG CACACCGGCC 
TGGACCGACC GTGTGCTGTG GAAAAGAAGA AAGTGGAACT TTGATAAAAC TGCGGAGGAG 
TTGGAGTTGA ATGTAGTAGG AGCTCCAGTG AATGAGGAAG AACAGTATCC ATGGAGCCCT 
GGAGACCTCA AGTATTACGG CAGAGCTGAG CTGAAAACCT CTGATCACAG ACCTGTGGTG 
GCCATCATAG ATGTGGACAT ACTTGAGGTG GATCCCGAGG CTCGTCATCA GGTGTATAAG 
GAAGTGATCG CCCTGCAGGG TCCTCCCGAC GGCACCATCC TAGTCTCTCT CTGCTCTTCT 
GGTCCCGATG ACTACTTTGA TGATGCTTTA ATTGACGATC TTTTGGACAA GTTTGCTAAT 
TTTGGCGAGG TTATTCTTAT CAGGTTTGTT GAAGAGAAAA TGTGGGTGAC ATTTTTGGAG 
GGATACTCAG CTCTGGCCGC TCTGTCTTTG AGTGGGTCTA CTGTGAATGG AAAGACCATA 
GACATCCGCC TGAGGAGTCC TGGATGGATA AAGAGTCTTG AGGAGGAAAT GAGTGTGGAG 
AGAATCTGTG GTAGTATTCC AACATCCACC AGCTCCACCC TGCTGGCAGA AAACTCTGAC 
CTAGGAGAAG AGTATGACAT GGAAGGTGAT GTGGACGAGG AGGTTGAGGA TATTTTACCC 
CAGCACCTGC AGCCTGGAGC AGGCATGGAT CTGAGTGCGT CTCCTGCCAC ATCTCCACGC 
ACCAGCCCAT GCCCTTCACC CACTCACGGA GAGCCAGCAC CACCCATCCG ACCCAGCAGA 
GCGCCACCAC GCACAGCCGG ACCACCACAG GGAGGATTAA GCCCAGCATC CATTAGAAGG 
GAACTGGCAG GTTCTCCAGT TGATGGTCAG CCAGCAGGAG CTCCATTCTC ACAGGGACTA 
GAGCCAAAAC GTCCCCCTCC GCCTCGCCCT AATGCCCCGC CAGCCCGACC TGCACCTCCA 
CAGCGCCCAC CACCACCCTC AGGTGGCAGA AGTCCCACAC CTGTTAGAAA AGATTCAGCA 
GGCTTCGCTG AGCCTCTCAA TCTAGAGTTT GATCTGACTG ATAATGAAGG ACAAAAAAGC 
CCTGCGTTAG CACGAGCAGA TGCAGCTGGT CGAGGTCAAG CCACCGGACC AGCTCCTGGA 
GGCATCCCAA GGCCGATCCC ACCCCGAGCA GGAGTCATCA GCGTCACGCC TCAGGCCAGA 
CCTCCTCCTC CTGCTCATCC CGGGGCCCCC AGACCCACAG CAGAGGTGCA TCCTGGAGCC 
CCTCGACCCT CACCAGATAA TCATCCCGGA GCTCCAAGAC CCACTGCTGA ACCCCAAAGC 
AAACCGTCTG AGCTGCCTCT GGGTCCACCC CTGACACTGC CAGGTCCCGT AAGGCCTCAG 
ATGACATCAC CCATGCAGCC TCAGTCTGTG TCGCCCGTTC AGCCTCCAGT CCAACCCCAA 
CTGCCCCCAC CCATACAATC CCAGCTCCCT CCACCAATGC AGCCCACCTT ACCTGCTCCT 
CTGATGCCTC AGCAGGCCCC TCAGACATCA GCCGGAGCCG GAGCCGCTGC CGCCCCTCAG 
CCTGGACTGG CATCTCCCAA ACCTCCACCC CGCAGCCGTT CCTCTCATGC ACTGCCACCT 
GAATCTGCTC CAGCTCCAAC AACACAGGCC AAGAACATGA ATGGAGTCCA AAGAGAAGCA 
CAATGGAACC TAGACCCCTT CGACATGCTT AACCCTCAAT CCCTCTTCCA GAACACACCA 
TTCACCGCTT CCCTCTCTCG CTCCTCATCC TCCTCTACAT CCACTCCATC CCCTTCTTCC 
TCATCCTCAA CGTTACCCAG CAACCTTTCC CTGTTCTCAG CTCCGGACAC CAGCAGCGCT 
TCGTGTCTCC TCGCTCCCCC CGCCCCATCC CGCAGCAAAT CCCAAGAGAC CCTCCGTTGC 
TCCCCTAATC CATTCCTCAC AGACATCCAA CCAAGACCCA ACAGCACCAA CCCCTTCACC 
AGCGGGCTGC AGTCCTCCCC GCGCCGATCG CTTACACCTG ATTTCTACAC CCAAATGCAA 
GCCTCTAAAC CTGATTTCAA TAGAGCTATG TCTGCTGTAG GCCACAGTTC CACCCTTCCT 
CCAACGTTCT CCCGACAACA GTCCTTAGTT ACTGCTACAG CTCCAGCCCC CAACAAACAA 
ACGCAAAAGT GGGTCACCTT TGATGATGAT TTGGATTTCC TTTCAAAAAG GGTTGGGACC 
GGCCCATTAG CTTCACCTCT TCCTTTTCCA CAAACCCAAA GCAGTTTCCC AAGCTCAGGC 
TTTGGCATGG ACAACAACTG GGCATCGCTT CCCACCTCTG CATTCCCAGC AATCCCTCCG 
CCTGTACCAA CCCGGACTAA TACCAGCATC AAAAACGCCC AACTCCCTTC CAGAAATGAT 
TTCACAGAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303582.1
CDS	118..4932
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_009305307.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305307.2

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGCCTCT CCAAGGGTGC TTCTAGGCAT GTGTCAGAAC 
CACTTCAAAT ACACTCGGCC CAAGAAGATC CGAGTATGTG TGGGAACGTG GAACGTAAAT 
GGAGGCAAAC AGTTTCGCAG CATTGCGTTT CGTAACCATA CGCTCAACGA CTGGCTTCTG 
GATGCTCCTA AGAAAGCCGG ACACCCAGAA TTTCAGGATG TCAAAAACAA CCCTGTGGAC 
ATCTTTGCTA TTGGTTTTGA GGAAATGGTG GAGCTGAATG CTGGTAATAT AGTCAGCGCA 
AGCACCACAA ACCAAAAGCT GTGGGCCGCA GAGCTGCAGA AGAACATCTC CCGAGATCAA 
CGATATGTCC TGCTGGCCTC AGAACAGCTG GTCGGTGTGT GTCTTTTCGT CTTCATTCGC 
CCTCAGCACG CACCCTTTAT CAGGGATGTT GCTGTAGACA CAGTGAAGAC TGGAATGGGC 
GGAGCCACTG GTAATAAGGG AGGCGTGGCT ATTCGTATGC TCTTCCACAC TACCAGCATC 
TGCTTCGTGT GTTCGCACTT TGCTGCCGGT CAATCACAAG TCAAAGAGAG GAACGACGAC 
TATAATGAGA TTGCACGCAA ACTGTCCTTC CCCATGGGTC GTCTTCTGTA CTCTCATGAT 
TATGTATTCT GGTGTGGAGA CTTCAACTAC CGAATCAATA TTCCCAATGA AGAGGTGAAG 
GAGCTGATCA GACAGCAGAA CTGGGATGCA CTTATTGGTG GAGATCAACT GGTGGAGCAG 
AAGAATGCAG GACAGGTCTT CAGAGGCTTT ATTGAAGGAA AGCTGGATTT TGCGCCCACT 
TATAAATACG ACCTTTTTTC TGAGGATTAC GACACTAGTG AGAAGTGCCG CACACCGGCC 
TGGACCGACC GTGTGCTGTG GAAAAGAAGA AAGTGGAACT TTGATAAAAC TGCGGAGGAG 
TTGGAGTTGA ATGTAGTAGG AGCTCCAGTG AATGAGGAAG AACAGTATCC ATGGAGCCCT 
GGAGACCTCA AGTATTACGG CAGAGCTGAG CTGAAAACCT CTGATCACAG ACCTGTGGTG 
GCCATCATAG ATGTGGACAT ACTTGAGGTG GATCCCGAGG CTCGTCATCA GGTGTATAAG 
GAAGTGATCG CCCTGCAGGG TCCTCCCGAC GGCACCATCC TAGTCTCTCT CTGCTCTTCT 
GGTCCCGATG ACTACTTTGA TGATGCTTTA ATTGACGATC TTTTGGACAA GTTTGCTAAT 
TTTGGCGAGG TTATTCTTAT CAGGTTTGTT GAAGAGAAAA TGTGGGTGAC ATTTTTGGAG 
GGATACTCAG CTCTGGCCGC TCTGTCTTTG AGTGGGTCTA CTGTGAATGG AAAGACCATA 
GACATCCGCC TGAGGAGTCC TGGATGGATA AAGAGTCTTG AGGAGGAAAT GAGTGTGGAG 
AGAATCTGTG GTAGTATTCC AACATCCACC AGCTCCACCC TGCTGGCAGA AAACTCTGAC 
CTAGGAGAAG AGTATGACAT GGAAGGTGAT GTGGACGAGG AGGTTGAGGA TATTTTACCC 
CAGCACCTGC AGCCTGGAGC AGGCATGGAT CTGAGTGCGT CTCCTGCCAC ATCTCCACGC 
ACCAGCCCAT GCCCTTCACC CACTCACGGA GAGCCAGCAC CACCCATCCG ACCCAGCAGA 
GCGCCACCAC GCACAGCCGG ACCACCACAG GGAGGATTAA GCCCAGCATC CATTAGAAGG 
GAACTGGCAG GTTCTCCAGT TGATGGTCAG CCAGCAGGAG CTCCATTCTC ACAGGGACTA 
GAGCCAAAAC GTCCCCCTCC GCCTCGCCCT AATGCCCCGC CAGCCCGACC TGCACCTCCA 
CAGCGCCCAC CACCACCCTC AGGTGGCAGA AGTCCCACAC CTGTTAGAAA AGATTCAGCA 
GGCTTCGCTG AGCCTCTCAA TCTAGAGTTT GATCTGACTG ATAATGAAGG ACAAAAAAGC 
CCTGCGTTAG CACGAGCAGA TGCAGCTGGT CGAGGTCAAG CCACCGGACC AGCTCCTGGA 
GGCATCCCAA GGCCGATCCC ACCCCGAGCA GGAGTCATCA GCGTCACGCC TCAGGCCAGA 
CCTCCTCCTC CTGCTCATCC CGGGGCCCCC AGACCCACAG CAGAGGTGCA TCCTGGAGCC 
CCTCGACCCT CACCAGATAA TCATCCCGGA GCTCCAAGAC CCACTGCTGA ACCCCAAAGC 
AAACCGTCTG AGCTGCCTCT GGGTCCACCC CTGACACTGC CAGGTCCCGT AAGGCCTCAG 
ATGACATCAC CCATGCAGCC TCAGTCTGTG TCGCCCGTTC AGCCTCCAGT CCAACCCCAA 
CTGCCCCCAC CCATACAATC CCAGCTCCCT CCACCAATGC AGCCCACCTT ACCTGCTCCT 
CTGATGCCTC AGCAGGCCCC TCAGACATCA GCCGGAGCCG GAGCCGCTGC CGCCCCTCAG 
CCTGGACTGG CATCTCCCAA ACCTCCACCC CGCAGCCGTT CCTCTCATGC ACTGCCACCT 
GAATCTGCTC CAGCTCCAAC AACACAGGCC AAGAACATGA ATGGAGTCCA AAGAGAAGCA 
CAATGGAACC TAGACCCCTT CGACATGCTT AACCCTCAAT CCCTCTTCCA GAACACACCA 
TTCACCGCTT CCCTCTCTCG CTCCTCATCC TCCTCTACAT CCACTCCATC CCCTTCTTCC 
TCATCCTCAA CGTTACCCAG CAACCTTTCC CTGTTCTCAG CTCCGGACAC CAGCAGCGCT 
TCGTGTCTCC TCGCTCCCCC CGCCCCATCC CGCAGCAAAT CCCAAGAGAC CCTCCGTTGC 
TCCCCTAATC CATTCCTCAC AGACATCCAA CCAAGACCCA ACAGCACCAA CCCCTTCACC 
AGCGGGCTGC AGTCCTCCCC GCGCCGATCG CTTACACCTG ATTTCTACAC CCAAATGCAA 
GCCTCTAAAC CTGATTTCAA TAGAGCTATG TCTGCTGTAG GCCACAGTTC CACCCTTCCT 
CCAACGTTCT CCCGACAACA GTCCTTAGTT ACTGCTACAG CTCCAGCCCC CAACAAACAA 
ACGCAAAAGT GGGTCACCTT TGATGATGAT TTGGATTTCC TTTCAAAAAG GGTTGGGACC 
GGCCCATTAG CTTCACCTCT TCCTTTTCCA CAAACCCAAA GCAGTTTCCC AAGCTCAGGC 
TTTGGCATGG ACAACAACTG GGCATCGCTT CCCACCTCTG CATTCCCAGC AATCCCTCCG 
CCTGTACCAA CCCGGACTAA TACCAGCATC AAAAACGCCC AACTCCCTTC CAGAAATGAT 
TTCACAGAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_009305307.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303582.1 Danio rerio synaptojanin 1 (synj1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa40105
Clone ID Related Accession (Same CDS sequence)	XM_009305307.2 , XM_009305307.3
Accession Version	XM_009305307.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptojanin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305307.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGCCTCT CCAAGGGTGC TTCTAGGCAT GTGTCAGAAC 
CACTTCAAAT ACACTCGGCC CAAGAAGATC CGAGTATGTG TGGGAACGTG GAACGTAAAT 
GGAGGCAAAC AGTTTCGCAG CATTGCGTTT CGTAACCATA CGCTCAACGA CTGGCTTCTG 
GATGCTCCTA AGAAAGCCGG ACACCCAGAA TTTCAGGATG TCAAAAACAA CCCTGTGGAC 
ATCTTTGCTA TTGGTTTTGA GGAAATGGTG GAGCTGAATG CTGGTAATAT AGTCAGCGCA 
AGCACCACAA ACCAAAAGCT GTGGGCCGCA GAGCTGCAGA AGAACATCTC CCGAGATCAA 
CGATATGTCC TGCTGGCCTC AGAACAGCTG GTCGGTGTGT GTCTTTTCGT CTTCATTCGC 
CCTCAGCACG CACCCTTTAT CAGGGATGTT GCTGTAGACA CAGTGAAGAC TGGAATGGGC 
GGAGCCACTG GTAATAAGGG AGGCGTGGCT ATTCGTATGC TCTTCCACAC TACCAGCATC 
TGCTTCGTGT GTTCGCACTT TGCTGCCGGT CAATCACAAG TCAAAGAGAG GAACGACGAC 
TATAATGAGA TTGCACGCAA ACTGTCCTTC CCCATGGGTC GTCTTCTGTA CTCTCATGAT 
TATGTATTCT GGTGTGGAGA CTTCAACTAC CGAATCAATA TTCCCAATGA AGAGGTGAAG 
GAGCTGATCA GACAGCAGAA CTGGGATGCA CTTATTGGTG GAGATCAACT GGTGGAGCAG 
AAGAATGCAG GACAGGTCTT CAGAGGCTTT ATTGAAGGAA AGCTGGATTT TGCGCCCACT 
TATAAATACG ACCTTTTTTC TGAGGATTAC GACACTAGTG AGAAGTGCCG CACACCGGCC 
TGGACCGACC GTGTGCTGTG GAAAAGAAGA AAGTGGAACT TTGATAAAAC TGCGGAGGAG 
TTGGAGTTGA ATGTAGTAGG AGCTCCAGTG AATGAGGAAG AACAGTATCC ATGGAGCCCT 
GGAGACCTCA AGTATTACGG CAGAGCTGAG CTGAAAACCT CTGATCACAG ACCTGTGGTG 
GCCATCATAG ATGTGGACAT ACTTGAGGTG GATCCCGAGG CTCGTCATCA GGTGTATAAG 
GAAGTGATCG CCCTGCAGGG TCCTCCCGAC GGCACCATCC TAGTCTCTCT CTGCTCTTCT 
GGTCCCGATG ACTACTTTGA TGATGCTTTA ATTGACGATC TTTTGGACAA GTTTGCTAAT 
TTTGGCGAGG TTATTCTTAT CAGGTTTGTT GAAGAGAAAA TGTGGGTGAC ATTTTTGGAG 
GGATACTCAG CTCTGGCCGC TCTGTCTTTG AGTGGGTCTA CTGTGAATGG AAAGACCATA 
GACATCCGCC TGAGGAGTCC TGGATGGATA AAGAGTCTTG AGGAGGAAAT GAGTGTGGAG 
AGAATCTGTG GTAGTATTCC AACATCCACC AGCTCCACCC TGCTGGCAGA AAACTCTGAC 
CTAGGAGAAG AGTATGACAT GGAAGGTGAT GTGGACGAGG AGGTTGAGGA TATTTTACCC 
CAGCACCTGC AGCCTGGAGC AGGCATGGAT CTGAGTGCGT CTCCTGCCAC ATCTCCACGC 
ACCAGCCCAT GCCCTTCACC CACTCACGGA GAGCCAGCAC CACCCATCCG ACCCAGCAGA 
GCGCCACCAC GCACAGCCGG ACCACCACAG GGAGGATTAA GCCCAGCATC CATTAGAAGG 
GAACTGGCAG GTTCTCCAGT TGATGGTCAG CCAGCAGGAG CTCCATTCTC ACAGGGACTA 
GAGCCAAAAC GTCCCCCTCC GCCTCGCCCT AATGCCCCGC CAGCCCGACC TGCACCTCCA 
CAGCGCCCAC CACCACCCTC AGGTGGCAGA AGTCCCACAC CTGTTAGAAA AGATTCAGCA 
GGCTTCGCTG AGCCTCTCAA TCTAGAGTTT GATCTGACTG ATAATGAAGG ACAAAAAAGC 
CCTGCGTTAG CACGAGCAGA TGCAGCTGGT CGAGGTCAAG CCACCGGACC AGCTCCTGGA 
GGCATCCCAA GGCCGATCCC ACCCCGAGCA GGAGTCATCA GCGTCACGCC TCAGGCCAGA 
CCTCCTCCTC CTGCTCATCC CGGGGCCCCC AGACCCACAG CAGAGGTGCA TCCTGGAGCC 
CCTCGACCCT CACCAGATAA TCATCCCGGA GCTCCAAGAC CCACTGCTGA ACCCCAAAGC 
AAACCGTCTG AGCTGCCTCT GGGTCCACCC CTGACACTGC CAGGTCCCGT AAGGCCTCAG 
ATGACATCAC CCATGCAGCC TCAGTCTGTG TCGCCCGTTC AGCCTCCAGT CCAACCCCAA 
CTGCCCCCAC CCATACAATC CCAGCTCCCT CCACCAATGC AGCCCACCTT ACCTGCTCCT 
CTGATGCCTC AGCAGGCCCC TCAGACATCA GCCGGAGCCG GAGCCGCTGC CGCCCCTCAG 
CCTGGACTGG CATCTCCCAA ACCTCCACCC CGCAGCCGTT CCTCTCATGC ACTGCCACCT 
GAATCTGCTC CAGCTCCAAC AACACAGGCC AAGAACATGA ATGGAGTCCA AAGAGAAGCA 
CAATGGAACC TAGACCCCTT CGACATGCTT AACCCTCAAT CCCTCTTCCA GAACACACCA 
TTCACCGCTT CCCTCTCTCG CTCCTCATCC TCCTCTACAT CCACTCCATC CCCTTCTTCC 
TCATCCTCAA CGTTACCCAG CAACCTTTCC CTGTTCTCAG CTCCGGACAC CAGCAGCGCT 
TCGTGTCTCC TCGCTCCCCC CGCCCCATCC CGCAGCAAAT CCCAAGAGAC CCTCCGTTGC 
TCCCCTAATC CATTCCTCAC AGACATCCAA CCAAGACCCA ACAGCACCAA CCCCTTCACC 
AGCGGGCTGC AGTCCTCCCC GCGCCGATCG CTTACACCTG ATTTCTACAC CCAAATGCAA 
GCCTCTAAAC CTGATTTCAA TAGAGCTATG TCTGCTGTAG GCCACAGTTC CACCCTTCCT 
CCAACGTTCT CCCGACAACA GTCCTTAGTT ACTGCTACAG CTCCAGCCCC CAACAAACAA 
ACGCAAAAGT GGGTCACCTT TGATGATGAT TTGGATTTCC TTTCAAAAAG GGTTGGGACC 
GGCCCATTAG CTTCACCTCT TCCTTTTCCA CAAACCCAAA GCAGTTTCCC AAGCTCAGGC 
TTTGGCATGG ACAACAACTG GGCATCGCTT CCCACCTCTG CATTCCCAGC AATCCCTCCG 
CCTGTACCAA CCCGGACTAA TACCAGCATC AAAAACGCCC AACTCCCTTC CAGAAATGAT 
TTCACAGAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303582.1
CDS	121..4935
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_009305307.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305307.3

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGCCTCT CCAAGGGTGC TTCTAGGCAT GTGTCAGAAC 
CACTTCAAAT ACACTCGGCC CAAGAAGATC CGAGTATGTG TGGGAACGTG GAACGTAAAT 
GGAGGCAAAC AGTTTCGCAG CATTGCGTTT CGTAACCATA CGCTCAACGA CTGGCTTCTG 
GATGCTCCTA AGAAAGCCGG ACACCCAGAA TTTCAGGATG TCAAAAACAA CCCTGTGGAC 
ATCTTTGCTA TTGGTTTTGA GGAAATGGTG GAGCTGAATG CTGGTAATAT AGTCAGCGCA 
AGCACCACAA ACCAAAAGCT GTGGGCCGCA GAGCTGCAGA AGAACATCTC CCGAGATCAA 
CGATATGTCC TGCTGGCCTC AGAACAGCTG GTCGGTGTGT GTCTTTTCGT CTTCATTCGC 
CCTCAGCACG CACCCTTTAT CAGGGATGTT GCTGTAGACA CAGTGAAGAC TGGAATGGGC 
GGAGCCACTG GTAATAAGGG AGGCGTGGCT ATTCGTATGC TCTTCCACAC TACCAGCATC 
TGCTTCGTGT GTTCGCACTT TGCTGCCGGT CAATCACAAG TCAAAGAGAG GAACGACGAC 
TATAATGAGA TTGCACGCAA ACTGTCCTTC CCCATGGGTC GTCTTCTGTA CTCTCATGAT 
TATGTATTCT GGTGTGGAGA CTTCAACTAC CGAATCAATA TTCCCAATGA AGAGGTGAAG 
GAGCTGATCA GACAGCAGAA CTGGGATGCA CTTATTGGTG GAGATCAACT GGTGGAGCAG 
AAGAATGCAG GACAGGTCTT CAGAGGCTTT ATTGAAGGAA AGCTGGATTT TGCGCCCACT 
TATAAATACG ACCTTTTTTC TGAGGATTAC GACACTAGTG AGAAGTGCCG CACACCGGCC 
TGGACCGACC GTGTGCTGTG GAAAAGAAGA AAGTGGAACT TTGATAAAAC TGCGGAGGAG 
TTGGAGTTGA ATGTAGTAGG AGCTCCAGTG AATGAGGAAG AACAGTATCC ATGGAGCCCT 
GGAGACCTCA AGTATTACGG CAGAGCTGAG CTGAAAACCT CTGATCACAG ACCTGTGGTG 
GCCATCATAG ATGTGGACAT ACTTGAGGTG GATCCCGAGG CTCGTCATCA GGTGTATAAG 
GAAGTGATCG CCCTGCAGGG TCCTCCCGAC GGCACCATCC TAGTCTCTCT CTGCTCTTCT 
GGTCCCGATG ACTACTTTGA TGATGCTTTA ATTGACGATC TTTTGGACAA GTTTGCTAAT 
TTTGGCGAGG TTATTCTTAT CAGGTTTGTT GAAGAGAAAA TGTGGGTGAC ATTTTTGGAG 
GGATACTCAG CTCTGGCCGC TCTGTCTTTG AGTGGGTCTA CTGTGAATGG AAAGACCATA 
GACATCCGCC TGAGGAGTCC TGGATGGATA AAGAGTCTTG AGGAGGAAAT GAGTGTGGAG 
AGAATCTGTG GTAGTATTCC AACATCCACC AGCTCCACCC TGCTGGCAGA AAACTCTGAC 
CTAGGAGAAG AGTATGACAT GGAAGGTGAT GTGGACGAGG AGGTTGAGGA TATTTTACCC 
CAGCACCTGC AGCCTGGAGC AGGCATGGAT CTGAGTGCGT CTCCTGCCAC ATCTCCACGC 
ACCAGCCCAT GCCCTTCACC CACTCACGGA GAGCCAGCAC CACCCATCCG ACCCAGCAGA 
GCGCCACCAC GCACAGCCGG ACCACCACAG GGAGGATTAA GCCCAGCATC CATTAGAAGG 
GAACTGGCAG GTTCTCCAGT TGATGGTCAG CCAGCAGGAG CTCCATTCTC ACAGGGACTA 
GAGCCAAAAC GTCCCCCTCC GCCTCGCCCT AATGCCCCGC CAGCCCGACC TGCACCTCCA 
CAGCGCCCAC CACCACCCTC AGGTGGCAGA AGTCCCACAC CTGTTAGAAA AGATTCAGCA 
GGCTTCGCTG AGCCTCTCAA TCTAGAGTTT GATCTGACTG ATAATGAAGG ACAAAAAAGC 
CCTGCGTTAG CACGAGCAGA TGCAGCTGGT CGAGGTCAAG CCACCGGACC AGCTCCTGGA 
GGCATCCCAA GGCCGATCCC ACCCCGAGCA GGAGTCATCA GCGTCACGCC TCAGGCCAGA 
CCTCCTCCTC CTGCTCATCC CGGGGCCCCC AGACCCACAG CAGAGGTGCA TCCTGGAGCC 
CCTCGACCCT CACCAGATAA TCATCCCGGA GCTCCAAGAC CCACTGCTGA ACCCCAAAGC 
AAACCGTCTG AGCTGCCTCT GGGTCCACCC CTGACACTGC CAGGTCCCGT AAGGCCTCAG 
ATGACATCAC CCATGCAGCC TCAGTCTGTG TCGCCCGTTC AGCCTCCAGT CCAACCCCAA 
CTGCCCCCAC CCATACAATC CCAGCTCCCT CCACCAATGC AGCCCACCTT ACCTGCTCCT 
CTGATGCCTC AGCAGGCCCC TCAGACATCA GCCGGAGCCG GAGCCGCTGC CGCCCCTCAG 
CCTGGACTGG CATCTCCCAA ACCTCCACCC CGCAGCCGTT CCTCTCATGC ACTGCCACCT 
GAATCTGCTC CAGCTCCAAC AACACAGGCC AAGAACATGA ATGGAGTCCA AAGAGAAGCA 
CAATGGAACC TAGACCCCTT CGACATGCTT AACCCTCAAT CCCTCTTCCA GAACACACCA 
TTCACCGCTT CCCTCTCTCG CTCCTCATCC TCCTCTACAT CCACTCCATC CCCTTCTTCC 
TCATCCTCAA CGTTACCCAG CAACCTTTCC CTGTTCTCAG CTCCGGACAC CAGCAGCGCT 
TCGTGTCTCC TCGCTCCCCC CGCCCCATCC CGCAGCAAAT CCCAAGAGAC CCTCCGTTGC 
TCCCCTAATC CATTCCTCAC AGACATCCAA CCAAGACCCA ACAGCACCAA CCCCTTCACC 
AGCGGGCTGC AGTCCTCCCC GCGCCGATCG CTTACACCTG ATTTCTACAC CCAAATGCAA 
GCCTCTAAAC CTGATTTCAA TAGAGCTATG TCTGCTGTAG GCCACAGTTC CACCCTTCCT 
CCAACGTTCT CCCGACAACA GTCCTTAGTT ACTGCTACAG CTCCAGCCCC CAACAAACAA 
ACGCAAAAGT GGGTCACCTT TGATGATGAT TTGGATTTCC TTTCAAAAAG GGTTGGGACC 
GGCCCATTAG CTTCACCTCT TCCTTTTCCA CAAACCCAAA GCAGTTTCCC AAGCTCAGGC 
TTTGGCATGG ACAACAACTG GGCATCGCTT CCCACCTCTG CATTCCCAGC AATCCCTCCG 
CCTGTACCAA CCCGGACTAA TACCAGCATC AAAAACGCCC AACTCCCTTC CAGAAATGAT 
TTCACAGAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_009305308.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303583.1 Danio rerio synaptojanin 1 (synj1), transcript variant X8, mRNA.

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CloneID	ODa40106
Clone ID Related Accession (Same CDS sequence)	XM_009305308.3 , XM_009305308.2
Accession Version	XM_009305308.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptojanin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305308.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGTCGA GGTCAAGCCA CCGGACCAGC TCCTGGAGGC 
ATCCCAAGGC CGATCCCACC CCGAGCAGGA GTCATCAGCG TCACGCCTCA GGCCAGACCT 
CCTCCTCCTG CTCATCCCGG GGCCCCCAGA CCCACAGCAG AGGTGCATCC TGGAGCCCCT 
CGACCCTCAC CAGATAATCA TCCCGGAGCT CCAAGACCCA CTGCTGAACC CCAAAGCAAA 
CCGTCTGAGC TGCCTCTGGG TCCACCCCTG ACACTGCCAG GTCCCGTAAG GCCTCAGATG 
ACATCACCCA TGCAGCCTCA GTCTGTGTCG CCCGTTCAGC CTCCAGTCCA ACCCCAACTG 
CCCCCACCCA TACAATCCCA GCTCCCTCCA CCAATGCAGC CCACCTTACC TGCTCCTCTG 
ATGCCTCAGC AGGCCCCTCA GACATCAGCC GGAGCCGGAG CCGCTGCCGC CCCTCAGCCT 
GGACTGGCAT CTCCCAAACC TCCACCCCGC AGCCGTTCCT CTCATGCACT GCCACCTGAA 
TCTGCTCCAG CTCCAACAAC ACAGGCCAAG AACATGAATG GAGTCCAAAG AGAAGCACAA 
TGGAACCTAG ACCCCTTCGA CATGCTTAAC CCTCAATCCC TCTTCCAGAA CACACCATTC 
ACCGCTTCCC TCTCTCGCTC CTCATCCTCC TCTACATCCA CTCCATCCCC TTCTTCCTCA 
TCCTCAACGT TACCCAGCAA CCTTTCCCTG TTCTCAGCTC CGGACACCAG CAGCGCTTCG 
TGTCTCCTCG CTCCCCCCGC CCCATCCCGC AGCAAATCCC AAGAGACCCT CCGTTGCTCC 
CCTAATCCAT TCCTCACAGA CATCCAACCA AGACCCAACA GCACCAACCC CTTCACCAGC 
GGGCTGCAGT CCTCCCCGCG CCGATCGCTT ACACCTGATT TCTACACCCA AATGCAAGCC 
TCTAAACCTG ATTTCAATAG AGCTATGTCT GCTGTAGGCC ACAGTTCCAC CCTTCCTCCA 
ACGTTCTCCC GACAACAGTC CTTAGTTACT GCTACAGCTC CAGCCCCCAA CAAACAAACG 
CAAAAGTGGG TCACCTTTGA TGATGATTTG GATTTCCTTT CAAAAAGGGT TGGGACCGGC 
CCATTAGCTT CACCTCTTCC TTTTCCACAA ACCCAAAGCA GTTTCCCAAG CTCAGGCTTT 
GGCATGGACA ACAACTGGGC ATCGCTTCCC ACCTCTGCAT TCCCAGCAAT CCCTCCGCCT 
GTACCAACCC GGACTAATAC CAGCATCAAA AACGCCCAAC TCCCTTCCAG AAATGATTTC 
ACAGAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303583.1
CDS	120..4871
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_009305308.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305308.3

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGTCGA GGTCAAGCCA CCGGACCAGC TCCTGGAGGC 
ATCCCAAGGC CGATCCCACC CCGAGCAGGA GTCATCAGCG TCACGCCTCA GGCCAGACCT 
CCTCCTCCTG CTCATCCCGG GGCCCCCAGA CCCACAGCAG AGGTGCATCC TGGAGCCCCT 
CGACCCTCAC CAGATAATCA TCCCGGAGCT CCAAGACCCA CTGCTGAACC CCAAAGCAAA 
CCGTCTGAGC TGCCTCTGGG TCCACCCCTG ACACTGCCAG GTCCCGTAAG GCCTCAGATG 
ACATCACCCA TGCAGCCTCA GTCTGTGTCG CCCGTTCAGC CTCCAGTCCA ACCCCAACTG 
CCCCCACCCA TACAATCCCA GCTCCCTCCA CCAATGCAGC CCACCTTACC TGCTCCTCTG 
ATGCCTCAGC AGGCCCCTCA GACATCAGCC GGAGCCGGAG CCGCTGCCGC CCCTCAGCCT 
GGACTGGCAT CTCCCAAACC TCCACCCCGC AGCCGTTCCT CTCATGCACT GCCACCTGAA 
TCTGCTCCAG CTCCAACAAC ACAGGCCAAG AACATGAATG GAGTCCAAAG AGAAGCACAA 
TGGAACCTAG ACCCCTTCGA CATGCTTAAC CCTCAATCCC TCTTCCAGAA CACACCATTC 
ACCGCTTCCC TCTCTCGCTC CTCATCCTCC TCTACATCCA CTCCATCCCC TTCTTCCTCA 
TCCTCAACGT TACCCAGCAA CCTTTCCCTG TTCTCAGCTC CGGACACCAG CAGCGCTTCG 
TGTCTCCTCG CTCCCCCCGC CCCATCCCGC AGCAAATCCC AAGAGACCCT CCGTTGCTCC 
CCTAATCCAT TCCTCACAGA CATCCAACCA AGACCCAACA GCACCAACCC CTTCACCAGC 
GGGCTGCAGT CCTCCCCGCG CCGATCGCTT ACACCTGATT TCTACACCCA AATGCAAGCC 
TCTAAACCTG ATTTCAATAG AGCTATGTCT GCTGTAGGCC ACAGTTCCAC CCTTCCTCCA 
ACGTTCTCCC GACAACAGTC CTTAGTTACT GCTACAGCTC CAGCCCCCAA CAAACAAACG 
CAAAAGTGGG TCACCTTTGA TGATGATTTG GATTTCCTTT CAAAAAGGGT TGGGACCGGC 
CCATTAGCTT CACCTCTTCC TTTTCCACAA ACCCAAAGCA GTTTCCCAAG CTCAGGCTTT 
GGCATGGACA ACAACTGGGC ATCGCTTCCC ACCTCTGCAT TCCCAGCAAT CCCTCCGCCT 
GTACCAACCC GGACTAATAC CAGCATCAAA AACGCCCAAC TCCCTTCCAG AAATGATTTC 
ACAGAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_009305308.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303583.1 Danio rerio synaptojanin 1 (synj1), transcript variant X8, mRNA.

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CloneID	ODa40106
Clone ID Related Accession (Same CDS sequence)	XM_009305308.3 , XM_009305308.2
Accession Version	XM_009305308.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synaptojanin-1 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305308.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGTCGA GGTCAAGCCA CCGGACCAGC TCCTGGAGGC 
ATCCCAAGGC CGATCCCACC CCGAGCAGGA GTCATCAGCG TCACGCCTCA GGCCAGACCT 
CCTCCTCCTG CTCATCCCGG GGCCCCCAGA CCCACAGCAG AGGTGCATCC TGGAGCCCCT 
CGACCCTCAC CAGATAATCA TCCCGGAGCT CCAAGACCCA CTGCTGAACC CCAAAGCAAA 
CCGTCTGAGC TGCCTCTGGG TCCACCCCTG ACACTGCCAG GTCCCGTAAG GCCTCAGATG 
ACATCACCCA TGCAGCCTCA GTCTGTGTCG CCCGTTCAGC CTCCAGTCCA ACCCCAACTG 
CCCCCACCCA TACAATCCCA GCTCCCTCCA CCAATGCAGC CCACCTTACC TGCTCCTCTG 
ATGCCTCAGC AGGCCCCTCA GACATCAGCC GGAGCCGGAG CCGCTGCCGC CCCTCAGCCT 
GGACTGGCAT CTCCCAAACC TCCACCCCGC AGCCGTTCCT CTCATGCACT GCCACCTGAA 
TCTGCTCCAG CTCCAACAAC ACAGGCCAAG AACATGAATG GAGTCCAAAG AGAAGCACAA 
TGGAACCTAG ACCCCTTCGA CATGCTTAAC CCTCAATCCC TCTTCCAGAA CACACCATTC 
ACCGCTTCCC TCTCTCGCTC CTCATCCTCC TCTACATCCA CTCCATCCCC TTCTTCCTCA 
TCCTCAACGT TACCCAGCAA CCTTTCCCTG TTCTCAGCTC CGGACACCAG CAGCGCTTCG 
TGTCTCCTCG CTCCCCCCGC CCCATCCCGC AGCAAATCCC AAGAGACCCT CCGTTGCTCC 
CCTAATCCAT TCCTCACAGA CATCCAACCA AGACCCAACA GCACCAACCC CTTCACCAGC 
GGGCTGCAGT CCTCCCCGCG CCGATCGCTT ACACCTGATT TCTACACCCA AATGCAAGCC 
TCTAAACCTG ATTTCAATAG AGCTATGTCT GCTGTAGGCC ACAGTTCCAC CCTTCCTCCA 
ACGTTCTCCC GACAACAGTC CTTAGTTACT GCTACAGCTC CAGCCCCCAA CAAACAAACG 
CAAAAGTGGG TCACCTTTGA TGATGATTTG GATTTCCTTT CAAAAAGGGT TGGGACCGGC 
CCATTAGCTT CACCTCTTCC TTTTCCACAA ACCCAAAGCA GTTTCCCAAG CTCAGGCTTT 
GGCATGGACA ACAACTGGGC ATCGCTTCCC ACCTCTGCAT TCCCAGCAAT CCCTCCGCCT 
GTACCAACCC GGACTAATAC CAGCATCAAA AACGCCCAAC TCCCTTCCAG AAATGATTTC 
ACAGAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303583.1
CDS	118..4869
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQAKNMNGVQREAQWNLDPFDMLNPQSLFQNTPFTASLSRSSSSSTSTPSPSSSSSTLPSNLSLFSAPDTSSASCLLAPPAPSRSKSQETLRCSPNPFLTDIQPRPNSTNPFTSGLQSSPRRSLTPDFYTQMQASKPDFNRAMSAVGHSSTLPPTFSRQQSLVTATAPAPNKQTQKWVTFDDDLDFLSKRVGTGPLASPLPFPQTQSSFPSSGFGMDNNWASLPTSAFPAIPPPVPTRTNTSIKNAQLPSRNDFTER

Target ORF information:

RefSeq Version	XM_009305308.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305308.2

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGTCGA GGTCAAGCCA CCGGACCAGC TCCTGGAGGC 
ATCCCAAGGC CGATCCCACC CCGAGCAGGA GTCATCAGCG TCACGCCTCA GGCCAGACCT 
CCTCCTCCTG CTCATCCCGG GGCCCCCAGA CCCACAGCAG AGGTGCATCC TGGAGCCCCT 
CGACCCTCAC CAGATAATCA TCCCGGAGCT CCAAGACCCA CTGCTGAACC CCAAAGCAAA 
CCGTCTGAGC TGCCTCTGGG TCCACCCCTG ACACTGCCAG GTCCCGTAAG GCCTCAGATG 
ACATCACCCA TGCAGCCTCA GTCTGTGTCG CCCGTTCAGC CTCCAGTCCA ACCCCAACTG 
CCCCCACCCA TACAATCCCA GCTCCCTCCA CCAATGCAGC CCACCTTACC TGCTCCTCTG 
ATGCCTCAGC AGGCCCCTCA GACATCAGCC GGAGCCGGAG CCGCTGCCGC CCCTCAGCCT 
GGACTGGCAT CTCCCAAACC TCCACCCCGC AGCCGTTCCT CTCATGCACT GCCACCTGAA 
TCTGCTCCAG CTCCAACAAC ACAGGCCAAG AACATGAATG GAGTCCAAAG AGAAGCACAA 
TGGAACCTAG ACCCCTTCGA CATGCTTAAC CCTCAATCCC TCTTCCAGAA CACACCATTC 
ACCGCTTCCC TCTCTCGCTC CTCATCCTCC TCTACATCCA CTCCATCCCC TTCTTCCTCA 
TCCTCAACGT TACCCAGCAA CCTTTCCCTG TTCTCAGCTC CGGACACCAG CAGCGCTTCG 
TGTCTCCTCG CTCCCCCCGC CCCATCCCGC AGCAAATCCC AAGAGACCCT CCGTTGCTCC 
CCTAATCCAT TCCTCACAGA CATCCAACCA AGACCCAACA GCACCAACCC CTTCACCAGC 
GGGCTGCAGT CCTCCCCGCG CCGATCGCTT ACACCTGATT TCTACACCCA AATGCAAGCC 
TCTAAACCTG ATTTCAATAG AGCTATGTCT GCTGTAGGCC ACAGTTCCAC CCTTCCTCCA 
ACGTTCTCCC GACAACAGTC CTTAGTTACT GCTACAGCTC CAGCCCCCAA CAAACAAACG 
CAAAAGTGGG TCACCTTTGA TGATGATTTG GATTTCCTTT CAAAAAGGGT TGGGACCGGC 
CCATTAGCTT CACCTCTTCC TTTTCCACAA ACCCAAAGCA GTTTCCCAAG CTCAGGCTTT 
GGCATGGACA ACAACTGGGC ATCGCTTCCC ACCTCTGCAT TCCCAGCAAT CCCTCCGCCT 
GTACCAACCC GGACTAATAC CAGCATCAAA AACGCCCAAC TCCCTTCCAG AAATGATTTC 
ACAGAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_009305309.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303584.1 Danio rerio synaptojanin 1 (synj1), transcript variant X11, mRNA.

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CloneID	ODa40107
Clone ID Related Accession (Same CDS sequence)	XM_009305309.2 , XM_009305309.3
Accession Version	XM_009305309.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synaptojanin-1 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305309.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGCTTC GCTGAGCCTC TCAATCTAGA GTTTGATCTG 
ACTGATAATG AAGGACAAAA AAGCCCTGCG TTAGCACGAG CAGATGCAGC TGGTCGAGGT 
CAAGCCACCG GACCAGCTCC TGGAGGCATC CCAAGGCCGA TCCCACCCCG AGCAGGAGTC 
ATCAGCGTCA CGCCTCAGGC CAGACCTCCT CCTCCTGCTC ATCCCGGGGC CCCCAGACCC 
ACAGCAGAGG TGCATCCTGG AGCCCCTCGA CCCTCACCAG ATAATCATCC CGGAGCTCCA 
AGACCCACTG CTGAACCCCA AAGCAAACCG TCTGAGCTGC CTCTGGGTCC ACCCCTGACA 
CTGCCAGGTC CCGTAAGGCC TCAGATGACA TCACCCATGC AGCCTCAGTC TGTGTCGCCC 
GTTCAGCCTC CAGTCCAACC CCAACTGCCC CCACCCATAC AATCCCAGCT CCCTCCACCA 
ATGCAGCCCA CCTTACCTGC TCCTCTGATG CCTCAGCAGG CCCCTCAGAC ATCAGCCGGA 
GCCGGAGCCG CTGCCGCCCC TCAGCCTGGA CTGGCATCTC CCAAACCTCC ACCCCGCAGC 
CGTTCCTCTC ATGCACTGCC ACCTGAATCT GCTCCAGCTC CAACAACACA GGAGCAATCC 
TCAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303584.1
CDS	118..4206
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQEQSSG

Target ORF information:

RefSeq Version	XM_009305309.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305309.2

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGCTTC GCTGAGCCTC TCAATCTAGA GTTTGATCTG 
ACTGATAATG AAGGACAAAA AAGCCCTGCG TTAGCACGAG CAGATGCAGC TGGTCGAGGT 
CAAGCCACCG GACCAGCTCC TGGAGGCATC CCAAGGCCGA TCCCACCCCG AGCAGGAGTC 
ATCAGCGTCA CGCCTCAGGC CAGACCTCCT CCTCCTGCTC ATCCCGGGGC CCCCAGACCC 
ACAGCAGAGG TGCATCCTGG AGCCCCTCGA CCCTCACCAG ATAATCATCC CGGAGCTCCA 
AGACCCACTG CTGAACCCCA AAGCAAACCG TCTGAGCTGC CTCTGGGTCC ACCCCTGACA 
CTGCCAGGTC CCGTAAGGCC TCAGATGACA TCACCCATGC AGCCTCAGTC TGTGTCGCCC 
GTTCAGCCTC CAGTCCAACC CCAACTGCCC CCACCCATAC AATCCCAGCT CCCTCCACCA 
ATGCAGCCCA CCTTACCTGC TCCTCTGATG CCTCAGCAGG CCCCTCAGAC ATCAGCCGGA 
GCCGGAGCCG CTGCCGCCCC TCAGCCTGGA CTGGCATCTC CCAAACCTCC ACCCCGCAGC 
CGTTCCTCTC ATGCACTGCC ACCTGAATCT GCTCCAGCTC CAACAACACA GGAGCAATCC 
TCAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synj1 ( XM_009305309.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303584.1 Danio rerio synaptojanin 1 (synj1), transcript variant X11, mRNA.

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CloneID	ODa40107
Clone ID Related Accession (Same CDS sequence)	XM_009305309.2 , XM_009305309.3
Accession Version	XM_009305309.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptojanin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305309.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGCTTC GCTGAGCCTC TCAATCTAGA GTTTGATCTG 
ACTGATAATG AAGGACAAAA AAGCCCTGCG TTAGCACGAG CAGATGCAGC TGGTCGAGGT 
CAAGCCACCG GACCAGCTCC TGGAGGCATC CCAAGGCCGA TCCCACCCCG AGCAGGAGTC 
ATCAGCGTCA CGCCTCAGGC CAGACCTCCT CCTCCTGCTC ATCCCGGGGC CCCCAGACCC 
ACAGCAGAGG TGCATCCTGG AGCCCCTCGA CCCTCACCAG ATAATCATCC CGGAGCTCCA 
AGACCCACTG CTGAACCCCA AAGCAAACCG TCTGAGCTGC CTCTGGGTCC ACCCCTGACA 
CTGCCAGGTC CCGTAAGGCC TCAGATGACA TCACCCATGC AGCCTCAGTC TGTGTCGCCC 
GTTCAGCCTC CAGTCCAACC CCAACTGCCC CCACCCATAC AATCCCAGCT CCCTCCACCA 
ATGCAGCCCA CCTTACCTGC TCCTCTGATG CCTCAGCAGG CCCCTCAGAC ATCAGCCGGA 
GCCGGAGCCG CTGCCGCCCC TCAGCCTGGA CTGGCATCTC CCAAACCTCC ACCCCGCAGC 
CGTTCCTCTC ATGCACTGCC ACCTGAATCT GCTCCAGCTC CAACAACACA GGAGCAATCC 
TCAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303584.1
CDS	120..4208
Translation	MAFSKGYRIYHKLDPPPYSVIVETRNREECLMFESGAVAVLSAAEKETIKASYTKMLDAYGILGVLRLNLGDSMLHSLVVVTGCSSVGKVQDSEVFRVTGTDFVSLKNDPTDEDRIADVRKVLNSGNFYFAWSSTGVSLDLSLNAHRRIREDTSDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAGHKQAKACVISRLSSERAGTRFNVRGTNDDGQVANFVETEQIIFLDDKVSSFIQIRGSIPLFWEQPGIQVGSHRVKLSRGFEANAPAFERHFSALKRLYGKQLIINLLGMKEGEHMLSKAFQSHLKASEHSNAVKMLNFDYHQMVKGGKTEKLQTVLKPQISKFVEDCDFFYYSGETGIQRCQSGTIRSNCLDCLDRTNSVQAFIALEMLPKQLEAMGLTEKPQLVARFQEVFRSMWSTNGDSISKIYAGTGALDGKAKGGKLKDGARSVTRTIQNNFFDSSKQEAIDILRLGSTLNSDLADKARALLTTSSLYVSEPILQSASPRVLLGMCQNHFKYTRPKKIRVCVGTWNVNGGKQFRSIAFRNHTLNDWLLDAPKKAGHPEFQDVKNNPVDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKNISRDQRYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGGVAIRMLFHTTSICFVCSHFAAGQSQVKERNDDYNEIARKLSFPMGRLLYSHDYVFWCGDFNYRINIPNEEVKELIRQQNWDALIGGDQLVEQKNAGQVFRGFIEGKLDFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWKRRKWNFDKTAEELELNVVGAPVNEEEQYPWSPGDLKYYGRAELKTSDHRPVVAIIDVDILEVDPEARHQVYKEVIALQGPPDGTILVSLCSSGPDDYFDDALIDDLLDKFANFGEVILIRFVEEKMWVTFLEGYSALAALSLSGSTVNGKTIDIRLRSPGWIKSLEEEMSVERICGSIPTSTSSTLLAENSDLGEEYDMEGDVDEEVEDILPQHLQPGAGMDLSASPATSPRTSPCPSPTHGEPAPPIRPSRAPPRTAGPPQGGLSPASIRRELAGSPVDGQPAGAPFSQGLEPKRPPPPRPNAPPARPAPPQRPPPPSGGRSPTPVRKDSAGFAEPLNLEFDLTDNEGQKSPALARADAAGRGQATGPAPGGIPRPIPPRAGVISVTPQARPPPPAHPGAPRPTAEVHPGAPRPSPDNHPGAPRPTAEPQSKPSELPLGPPLTLPGPVRPQMTSPMQPQSVSPVQPPVQPQLPPPIQSQLPPPMQPTLPAPLMPQQAPQTSAGAGAAAAPQPGLASPKPPPRSRSSHALPPESAPAPTTQEQSSG

Target ORF information:

RefSeq Version	XM_009305309.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptojanin 1 (synj1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305309.3

ORF Insert Sequence:

ATGGCATTCA GTAAAGGTTA TCGTATTTAT CACAAGCTGG ATCCACCTCC ATACAGTGTG 
ATTGTGGAGA CCAGAAATCG AGAAGAATGT CTGATGTTCG AGTCTGGAGC TGTTGCTGTA 
CTGTCGGCGG CAGAGAAAGA GACCATAAAA GCATCGTACA CCAAGATGCT GGATGCCTAT 
GGGATCCTGG GAGTTCTGCG TCTGAACCTT GGTGATTCTA TGCTGCACAG TCTGGTGGTC 
GTAACAGGCT GCAGCTCAGT GGGAAAAGTC CAGGATTCAG AAGTTTTTCG GGTAACAGGC 
ACAGACTTTG TCTCTCTGAA GAATGACCCC ACGGATGAGG ACCGGATCGC TGATGTCAGG 
AAAGTCTTAA ACTCTGGGAA CTTCTATTTC GCCTGGTCCT CAACTGGAGT GAGTCTGGAT 
CTCAGCTTGA ATGCCCATCG CAGAATCAGA GAAGACACAT CCGACAATCG CTTTTTCTGG 
AATCAGTCCC TTCATCTCCA TCTCAAGCAT TATGGGGTGA ACTGTGACGA CTGGCTTCTG 
AGATTGATGT GTGGTGGCGT GGAGATCCGC ACCATCTATG CTGGGCACAA ACAGGCCAAA 
GCTTGTGTGA TTTCTCGGCT CAGTTCGGAG AGAGCAGGCA CACGTTTCAA TGTCCGAGGC 
ACGAATGATG ATGGCCAGGT GGCCAATTTT GTGGAGACTG AGCAGATTAT TTTCCTTGAT 
GACAAAGTAT CCTCCTTCAT TCAGATCCGA GGGTCAATAC CGCTATTCTG GGAGCAGCCG 
GGAATTCAGG TTGGATCCCA CCGCGTTAAG CTGTCCCGTG GTTTTGAAGC CAACGCGCCG 
GCTTTTGAAA GACATTTCAG TGCTCTGAAA AGGCTGTATG GCAAACAGTT GATCATCAAT 
CTTCTGGGAA TGAAGGAGGG TGAACACATG CTCAGCAAAG CATTCCAGAG TCACCTCAAA 
GCTTCAGAGC ATTCCAACGC TGTGAAAATG TTGAACTTTG ACTATCATCA GATGGTTAAA 
GGAGGGAAGA CTGAGAAGCT TCAGACTGTG CTGAAACCTC AGATCAGCAA GTTTGTGGAG 
GATTGTGATT TCTTCTACTA CTCAGGGGAA ACCGGCATTC AGAGATGTCA AAGCGGAACC 
ATCCGTTCCA ACTGTCTGGA CTGCTTGGAC CGAACAAACA GCGTCCAGGC CTTTATTGCA 
CTTGAGATGC TTCCCAAACA GCTGGAAGCC ATGGGTTTGA CAGAGAAACC TCAGCTGGTG 
GCTCGTTTTC AGGAGGTTTT CCGCTCCATG TGGTCCACCA ATGGAGACTC CATCAGCAAG 
ATCTACGCAG GCACTGGTGC TTTAGATGGC AAGGCTAAGG GCGGAAAGCT AAAAGATGGA 
GCTCGCTCGG TCACCAGAAC CATCCAGAAC AACTTCTTTG ACAGCTCTAA ACAGGAAGCC 
ATTGACATCC TGAGACTGGG CAGCACCCTT AACAGTGACC TGGCAGATAA AGCACGAGCA 
CTTCTCACGA CAAGCAGCCT ATATGTCTCT GAGCCAATTT TACAGTCAGC CTCTCCAAGG 
GTGCTTCTAG GCATGTGTCA GAACCACTTC AAATACACTC GGCCCAAGAA GATCCGAGTA 
TGTGTGGGAA CGTGGAACGT AAATGGAGGC AAACAGTTTC GCAGCATTGC GTTTCGTAAC 
CATACGCTCA ACGACTGGCT TCTGGATGCT CCTAAGAAAG CCGGACACCC AGAATTTCAG 
GATGTCAAAA ACAACCCTGT GGACATCTTT GCTATTGGTT TTGAGGAAAT GGTGGAGCTG 
AATGCTGGTA ATATAGTCAG CGCAAGCACC ACAAACCAAA AGCTGTGGGC CGCAGAGCTG 
CAGAAGAACA TCTCCCGAGA TCAACGATAT GTCCTGCTGG CCTCAGAACA GCTGGTCGGT 
GTGTGTCTTT TCGTCTTCAT TCGCCCTCAG CACGCACCCT TTATCAGGGA TGTTGCTGTA 
GACACAGTGA AGACTGGAAT GGGCGGAGCC ACTGGTAATA AGGGAGGCGT GGCTATTCGT 
ATGCTCTTCC ACACTACCAG CATCTGCTTC GTGTGTTCGC ACTTTGCTGC CGGTCAATCA 
CAAGTCAAAG AGAGGAACGA CGACTATAAT GAGATTGCAC GCAAACTGTC CTTCCCCATG 
GGTCGTCTTC TGTACTCTCA TGATTATGTA TTCTGGTGTG GAGACTTCAA CTACCGAATC 
AATATTCCCA ATGAAGAGGT GAAGGAGCTG ATCAGACAGC AGAACTGGGA TGCACTTATT 
GGTGGAGATC AACTGGTGGA GCAGAAGAAT GCAGGACAGG TCTTCAGAGG CTTTATTGAA 
GGAAAGCTGG ATTTTGCGCC CACTTATAAA TACGACCTTT TTTCTGAGGA TTACGACACT 
AGTGAGAAGT GCCGCACACC GGCCTGGACC GACCGTGTGC TGTGGAAAAG AAGAAAGTGG 
AACTTTGATA AAACTGCGGA GGAGTTGGAG TTGAATGTAG TAGGAGCTCC AGTGAATGAG 
GAAGAACAGT ATCCATGGAG CCCTGGAGAC CTCAAGTATT ACGGCAGAGC TGAGCTGAAA 
ACCTCTGATC ACAGACCTGT GGTGGCCATC ATAGATGTGG ACATACTTGA GGTGGATCCC 
GAGGCTCGTC ATCAGGTGTA TAAGGAAGTG ATCGCCCTGC AGGGTCCTCC CGACGGCACC 
ATCCTAGTCT CTCTCTGCTC TTCTGGTCCC GATGACTACT TTGATGATGC TTTAATTGAC 
GATCTTTTGG ACAAGTTTGC TAATTTTGGC GAGGTTATTC TTATCAGGTT TGTTGAAGAG 
AAAATGTGGG TGACATTTTT GGAGGGATAC TCAGCTCTGG CCGCTCTGTC TTTGAGTGGG 
TCTACTGTGA ATGGAAAGAC CATAGACATC CGCCTGAGGA GTCCTGGATG GATAAAGAGT 
CTTGAGGAGG AAATGAGTGT GGAGAGAATC TGTGGTAGTA TTCCAACATC CACCAGCTCC 
ACCCTGCTGG CAGAAAACTC TGACCTAGGA GAAGAGTATG ACATGGAAGG TGATGTGGAC 
GAGGAGGTTG AGGATATTTT ACCCCAGCAC CTGCAGCCTG GAGCAGGCAT GGATCTGAGT 
GCGTCTCCTG CCACATCTCC ACGCACCAGC CCATGCCCTT CACCCACTCA CGGAGAGCCA 
GCACCACCCA TCCGACCCAG CAGAGCGCCA CCACGCACAG CCGGACCACC ACAGGGAGGA 
TTAAGCCCAG CATCCATTAG AAGGGAACTG GCAGGTTCTC CAGTTGATGG TCAGCCAGCA 
GGAGCTCCAT TCTCACAGGG ACTAGAGCCA AAACGTCCCC CTCCGCCTCG CCCTAATGCC 
CCGCCAGCCC GACCTGCACC TCCACAGCGC CCACCACCAC CCTCAGGTGG CAGAAGTCCC 
ACACCTGTTA GAAAAGATTC AGCAGGCTTC GCTGAGCCTC TCAATCTAGA GTTTGATCTG 
ACTGATAATG AAGGACAAAA AAGCCCTGCG TTAGCACGAG CAGATGCAGC TGGTCGAGGT 
CAAGCCACCG GACCAGCTCC TGGAGGCATC CCAAGGCCGA TCCCACCCCG AGCAGGAGTC 
ATCAGCGTCA CGCCTCAGGC CAGACCTCCT CCTCCTGCTC ATCCCGGGGC CCCCAGACCC 
ACAGCAGAGG TGCATCCTGG AGCCCCTCGA CCCTCACCAG ATAATCATCC CGGAGCTCCA 
AGACCCACTG CTGAACCCCA AAGCAAACCG TCTGAGCTGC CTCTGGGTCC ACCCCTGACA 
CTGCCAGGTC CCGTAAGGCC TCAGATGACA TCACCCATGC AGCCTCAGTC TGTGTCGCCC 
GTTCAGCCTC CAGTCCAACC CCAACTGCCC CCACCCATAC AATCCCAGCT CCCTCCACCA 
ATGCAGCCCA CCTTACCTGC TCCTCTGATG CCTCAGCAGG CCCCTCAGAC ATCAGCCGGA 
GCCGGAGCCG CTGCCGCCCC TCAGCCTGGA CTGGCATCTC CCAAACCTCC ACCCCGCAGC 
CGTTCCTCTC ATGCACTGCC ACCTGAATCT GCTCCAGCTC CAACAACACA GGAGCAATCC 
TCAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	synj1
Entrez Gene ID	337236
Full Name	synaptojanin 1
Synonyms	fb02f11,fi15a11,nrc,wu:fb02f11,wu:fi15a11
General protein information	Preferred Names synaptojanin 1 Names synaptojanin-1 no optokinetic response c slacker slak
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Homo sapiens (human)	SYNJ1	NP_003886.3
Pan troglodytes (chimpanzee)	SYNJ1	XP_531429.2
Gallus gallus (chicken)	SYNJ1	XP_416706.3
Xenopus tropicalis (tropical clawed frog)	synj1	XP_004912203.1
Canis lupus familiaris (dog)	SYNJ1	XP_005638893.1
Caenorhabditis elegans (roundworm)	unc-26	NP_001023266.1
Rattus norvegicus (Norway rat)	Synj1	NP_445928.2
Danio rerio (zebrafish)	synj1	NP_001007031.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012175	XP_320360.4
Caenorhabditis elegans (roundworm)	unc-26	NP_001129862.1
	SYNJ1	XP_002803210.1
Bos taurus (cattle)	SYNJ1	NP_776893.2
Mus musculus (house mouse)	Synj1	NP_001157955.1
Drosophila melanogaster (fruit fly)	synj	NP_001246454.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

synj1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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