Synj cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Synj
Entrez Gene ID	37517
Full Name	Synaptojanin
Synonyms	CG6562,Dmel\CG6562,IPP,synj
General protein information	Preferred Names Synaptojanin Names CG6562 gene product from transcript CG6562-RB CG6562-PA CG6562-PB CG6562-PC Synj-PA Synj-PB Synj-PC synaptojanin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 58B4-58B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259525.1	NP_001246454.1	synaptojanin, isoform C
NM_130462.4	NP_569729.1	synaptojanin, isoform B
NM_166505.2	NP_726155.1	synaptojanin, isoform A
NM_166505.1	NP_726155.1	synaptojanin, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	SYNJ1	NP_003886.3
Pan troglodytes (chimpanzee)	SYNJ1	XP_531429.2
Gallus gallus (chicken)	SYNJ1	XP_416706.3
Xenopus tropicalis (tropical clawed frog)	synj1	XP_004912203.1
Canis lupus familiaris (dog)	SYNJ1	XP_005638893.1
Caenorhabditis elegans (roundworm)	unc-26	NP_001023266.1
Rattus norvegicus (Norway rat)	Synj1	NP_445928.2
Danio rerio (zebrafish)	synj1	NP_001007031.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012175	XP_320360.4
Caenorhabditis elegans (roundworm)	unc-26	NP_001129862.1
	SYNJ1	XP_002803210.1
Bos taurus (cattle)	SYNJ1	NP_776893.2
Mus musculus (house mouse)	Synj1	NP_001157955.1
Drosophila melanogaster (fruit fly)	synj	NP_001246454.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated that Synaptojanin, a protein with a known role in synaptic vesicle endocytosis, is phosphorylated at S1029 in vivo by the Minibrain kinase. We further demonstrate that the phosphorylation status of Synaptojanin at S1029 differentially regulates its participation in the recycling of distinct synaptic vesicle pools.
Title: Phosphorylation of Synaptojanin Differentially Regulates Endocytosis of Functionally Distinct Synaptic Vesicle Pools.

Mnb is a synaptic kinase that promotes efficient synaptic vesicle recycling by Synj function at the neuromuscular junction.
Title: Activity-dependent facilitation of Synaptojanin and synaptic vesicle recycling by the Minibrain kinase.

Findings imply that restoring the level of any one of these genes dap160/itsn1, synj/synj1, and nla/dscr1 may reduce endocytic defects seen in DS.
Title: Upregulation of three Drosophila homologs of human chromosome 21 genes alters synaptic function: implications for Down syndrome.

In the absence of synaptojanin or endophilin, vesicles undergo full fusion and re-formation.
Title: A slowed classical pathway rather than kiss-and-run mediates endocytosis at synapses lacking synaptojanin and endophilin.

Synjanin is mislocalized and destabilized at synapses devoid of Endophilin, suggesting that Endophilin recruits and stabilizes Synjanin on newly formed vesicles to promote vesicle uncoating.
Title: Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating.

The following Synj gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Synj cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10878	NM_001259525.1 Latest version!	Drosophila melanogaster synaptojanin, transcript variant C (Synj), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa10878	NM_130462.4 Latest version!	Drosophila melanogaster synaptojanin, transcript variant B (Synj), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa10878	NM_166505.2 Latest version!	Drosophila melanogaster synaptojanin, transcript variant A (Synj), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OFa10878	NM_166505.1	Drosophila melanogaster synaptojanin, transcript variant A (Synj), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Synj ( NM_001259525.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246454.1 Drosophila melanogaster synaptojanin, transcript variant C (Synj), mRNA.

Price & Availability↑

CloneID	OFa10878
Clone ID Related Accession (Same CDS sequence)	NM_001259525.1 , NM_130462.4 , NM_166505.2 , NM_166505.1
Accession Version	NM_001259525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	synaptojanin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATGT CCAAGGTGAT CCGTGTGCTG GAGAAGTCCA TTGCCCCCTC GCCGCACAGC 
GTATTACTGG AGCATCGGAA CAAGAGCGAC AGCATCCTGT TCGAGTCCCA TGCGGTGGCC 
CTGCTGACCC AGCAGGAGAC GGATGTCATC CGGAAGCAGT ACACCAAGGT CTGCGACGCC 
TACGGATGTC TGGGTGCCCT CCAACTAAAC GCCGGCGAGA GCACCGTGCT GTTCCTGGTG 
CTGGTCACCG GCTGTGTGTC CATGGGCAAG ATCGGCGACA TCGAGATCTT CCGGATCACA 
CAAACCACTT TTGTCTCGCT ACAAAATGCA GCGCCCAACG AAGACAAGAT CAGCGAGGTG 
CGCAAGCTGC TCAACTCGGG CACCTTCTAC TTTGCCCACA CCAATGCCAG TGCATCGGCA 
TCCGGAGCGT CATCGTATCG GTTCGATATT ACGCTTTGCG CCCAGCGACG CCAGCAAACG 
CAGGAGACGG ACAACCGTTT CTTCTGGAAT CGCATGATGC ACATCCACCT GATGCGCTTC 
GGTATCGATT GCCAGTCCTG GTTGTTGCAA GCCATGTGCG GCTCCGTAGA AGTGCGCACC 
GTCTACATCG GTGCCAAACA GGCCCGTGCC GCCATCATTT CCCGACTGAG CTGCGAACGG 
GCTGGCACGC GTTTCAATGT CCGTGGTACC AACGATGAGG GCTATGTGGC TAACTTTGTG 
GAAACCGAGC AAGTGATCTA CGTGGACGGC GATGTTACTA GTTATGTGCA GACGCGAGGA 
TCGGTGCCAC TCTTCTGGGA ACAGCCAGGC GTCCAGGTTG GCTCGCACAA GGTGAAGCTA 
TCACGAGGAT TCGAGACATC GGCCGCCGCC TTTGATCGAC ACATGAGCAT GATGAGGCAA 
CGTTACGGCT ATCAGACGGT GGTGAATCTG CTAGGCAGCT CCCTTGTTGG CAGCAAGGAG 
GGCGAGGCCA TGCTGAGTAA TGAGTTCCAG CGTCATCACG GCATGTCAGC CCACAAGGAT 
GTGCCGCATG TGGTGTTTGA CTATCATCAG GAGTGTCGCG GCGGCAATTT CTCGGCGCTG 
GCCAAGCTCA AGGAACGGAT CGTAGCCTGC GGTGCTAACT ACGGCGTCTT CCACGCGTCC 
AATGGTCAGG TGTTGCGCGA GCAGTTCGGT GTTGTGCGCA CGAATTGCCT AGACTGTTTG 
GACAGGACAA ACTGTGTGCA GACGTATCTC GGGCTTGACA CGCTCGGTAT CCAGCTAGAG 
GCTTTGAAAA TGGGCGGCAA GCAGCAGAAT ATTTCGCGGT TTGAGGAGAT CTTCCGACAG 
ATGTGGATCA ACAACGGAAA TGAGGTCAGC AAGATCTACG CCGGCACCGG GGCCATCCAG 
GGGGGATCAA AACTAATGGA TGGTGCGCGA TCTGCAGCAA GAACAATTCA AAATAACCTA 
CTGGACAACT CAAAGCAGGA AGCCATTGAT GTCCTGCTAG TGGGCTCCAC GCTTAGCTCG 
GAGCTTGCGG ATCGGGCTCG CATCCTACTG CCCTCCAATA TGTTGCATGC ACCTACCACT 
GTGTTGAGAG AGCTATGCAA GCGCTACACT GAATATGTGC GTCCTCGAAT GGCACGTGTA 
GCCGTGGGTA CCTATAACGT CAACGGCGGC AAGCACTTCC GCAGCATTGT GTTCAAGGAT 
TCGCTGGCCG ATTGGCTGCT CGACTGCCAT GCCCTTGCCC GCTCCAAGGC GCTTGTAGAT 
GTGAACAATC CGTCGGAGAA CGTCGATCAT CCGGTGGATA TCTACGCCAT TGGATTCGAG 
GAGATTGTGG ATCTGAATGC TTCCAACATA ATGGCGGCCA GCACCGACAA TGCCAAGTTG 
TGGGCCGAGG AGCTGCAGAA AACGATCTCG CGGGACAATG ACTACGTGCT GCTCACATAC 
CAGCAACTGG TGGGCGTGTG CCTATACATC TACATCCGAC CGGAGCACGC GCCGCACATC 
CGGGACGTGG CCATCGACTG TGTTAAGACA GGATTGGGTG GTGCCACTGG GAATAAGGGT 
GCCTGTGCCA TTCGATTTGT GCTTCATGGT ACTTCCATGT GCTTCGTGTG TGCCCACTTT 
GCAGCCGGAC AGTCACAGGT GGCTGAAAGG AACGCTGACT ACGCGGAAAT CACCCGGAAG 
CTGGCCTTCC CGATGGGCAG GACGCTAAAA TCACACGACT GGGTGTTTTG GTGCGGCGAC 
TTCAACTATC GCATCGACAT GGAGAAGGAC GAATTAAAGG AGTGCGTACG TAATGGAGAT 
CTCTCAACCG TCCTCGAGTT CGATCAATTG CGCAAGGAGC AGGAGGCTGG CAATGTGTTT 
GGCGAATTCC TCGAGGGAGA GATCACTTTC GACCCGACGT ACAAGTATGA TTTGTTCAGC 
GACGACTACG ACACCTCGGA GAAACAGCGA GCGCCCGCCT GGACAGATCG GGTTCTCTGG 
CGTCGCAGGA AGGCGCTGGC CGAGGGCGAC TTTGCTGCCT CAGCCTGGAA TCCCGGCAAA 
TTGATTCACT ACGGTCGTTC GGAGCTAAAG CAGAGTGATC ATAGACCCGT GATCGCTATT 
ATTGATGCTG AGATAATGGA GATCGATCAG CAGCGGAGAC GTGCTGTATT CGAGCAGGTT 
ATCCGAGATC TGGGTCCGCC GGACTCCACG ATTGTGGTAC ATGTCCTGGA GTCTTCAGCA 
ACTGGAGATG AGGATGGACC AACCATATAC GACGAGAATG TGATGTCAGC CCTGATTACC 
GAGCTATCCA AGATGGGAGA GGTCACTTTG GTGCGCTATG TGGAGGACAC CATGTGGGTC 
ACATTTCGGG ATGGAGAATC GGCTTTAAAT GCGTCTTCTA AGAAGAGTAT CCAAGTATGC 
GGATTAGATC TTATCCTGGA GCTGAAGTCA AAGGACTGGC AGCATCTGGT GGACAGCGAG 
ATAGAGCTAT GCACCACGAA CACCATACCG CTGTGCGCTA ATCCTGTAGA GCATGCACAA 
CTCCTGCAGG CCATTACGCC GGAGTTGCCC CAGCGGCCCA AGCAGCCGCC CACACGTCCC 
CCAGCCCGTC CCCCAATGCC TATGTCGCCA AAGAACTCAC CACGCCACCT GCCCCACGTC 
GGGGTCATCA GCATTGTGCC CAAGCCGGCA AAGCCACCGA TGCCACCGCA ACCGCAATCT 
CAACCTCTAA TTCCGTCGCC GCTTCAGCCG CAGGTGGCGC CGCCTCGTCC GCCGGCAATG 
GACACCACGC CATCCTCCAA ATCGCAATCG CCAACGGAAC TTGTATCCGC TAGTTCTTCG 
ACGTCCTCTT CGGGAAAGAC TTCGCCCACA GCTCCAGCTG TGCTCTCCGG TCCGCCGACG 
CCGCCGCGCC AAATGCAGAG CAAGCAGGCC ACACCCGTCT CGACGCCCAC GCGCCAAGTC 
GGTTCGCCCA AGGATCCGAT TCCATCTGAT ACCGCCTACG AAACCGCCAG TAATATTTAC 
GAGGAGATTC AGGAGGATGT GCCTGCGCCA CGCCATCCGC CGCCGGCTGG ACCGCCGCCA 
GTGATAGGAG CACCAATGGG CCCGCCGCCA CCGCTGCCGA ATCGCAGGGG CCCACCACCC 
ATACCAAATA GGAGCGGCAA TGCTCCACCG CTGCCCACGC GACCCGCCAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246454.1
CDS	185..3841
Translation	MAMSKVIRVLEKSIAPSPHSVLLEHRNKSDSILFESHAVALLTQQETDVIRKQYTKVCDAYGCLGALQLNAGESTVLFLVLVTGCVSMGKIGDIEIFRITQTTFVSLQNAAPNEDKISEVRKLLNSGTFYFAHTNASASASGASSYRFDITLCAQRRQQTQETDNRFFWNRMMHIHLMRFGIDCQSWLLQAMCGSVEVRTVYIGAKQARAAIISRLSCERAGTRFNVRGTNDEGYVANFVETEQVIYVDGDVTSYVQTRGSVPLFWEQPGVQVGSHKVKLSRGFETSAAAFDRHMSMMRQRYGYQTVVNLLGSSLVGSKEGEAMLSNEFQRHHGMSAHKDVPHVVFDYHQECRGGNFSALAKLKERIVACGANYGVFHASNGQVLREQFGVVRTNCLDCLDRTNCVQTYLGLDTLGIQLEALKMGGKQQNISRFEEIFRQMWINNGNEVSKIYAGTGAIQGGSKLMDGARSAARTIQNNLLDNSKQEAIDVLLVGSTLSSELADRARILLPSNMLHAPTTVLRELCKRYTEYVRPRMARVAVGTYNVNGGKHFRSIVFKDSLADWLLDCHALARSKALVDVNNPSENVDHPVDIYAIGFEEIVDLNASNIMAASTDNAKLWAEELQKTISRDNDYVLLTYQQLVGVCLYIYIRPEHAPHIRDVAIDCVKTGLGGATGNKGACAIRFVLHGTSMCFVCAHFAAGQSQVAERNADYAEITRKLAFPMGRTLKSHDWVFWCGDFNYRIDMEKDELKECVRNGDLSTVLEFDQLRKEQEAGNVFGEFLEGEITFDPTYKYDLFSDDYDTSEKQRAPAWTDRVLWRRRKALAEGDFAASAWNPGKLIHYGRSELKQSDHRPVIAIIDAEIMEIDQQRRRAVFEQVIRDLGPPDSTIVVHVLESSATGDEDGPTIYDENVMSALITELSKMGEVTLVRYVEDTMWVTFRDGESALNASSKKSIQVCGLDLILELKSKDWQHLVDSEIELCTTNTIPLCANPVEHAQLLQAITPELPQRPKQPPTRPPARPPMPMSPKNSPRHLPHVGVISIVPKPAKPPMPPQPQSQPLIPSPLQPQVAPPRPPAMDTTPSSKSQSPTELVSASSSTSSSGKTSPTAPAVLSGPPTPPRQMQSKQATPVSTPTRQVGSPKDPIPSDTAYETASNIYEEIQEDVPAPRHPPPAGPPPVIGAPMGPPPPLPNRRGPPPIPNRSGNAPPLPTRPANN

Target ORF information:

RefSeq Version	NM_001259525.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster synaptojanin, transcript variant C (Synj), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259525.1

ORF Insert Sequence:

ATGGCCATGT CCAAGGTGAT CCGTGTGCTG GAGAAGTCCA TTGCCCCCTC GCCGCACAGC 
GTATTACTGG AGCATCGGAA CAAGAGCGAC AGCATCCTGT TCGAGTCCCA TGCGGTGGCC 
CTGCTGACCC AGCAGGAGAC GGATGTCATC CGGAAGCAGT ACACCAAGGT CTGCGACGCC 
TACGGATGTC TGGGTGCCCT CCAACTAAAC GCCGGCGAGA GCACCGTGCT GTTCCTGGTG 
CTGGTCACCG GCTGTGTGTC CATGGGCAAG ATCGGCGACA TCGAGATCTT CCGGATCACA 
CAAACCACTT TTGTCTCGCT ACAAAATGCA GCGCCCAACG AAGACAAGAT CAGCGAGGTG 
CGCAAGCTGC TCAACTCGGG CACCTTCTAC TTTGCCCACA CCAATGCCAG TGCATCGGCA 
TCCGGAGCGT CATCGTATCG GTTCGATATT ACGCTTTGCG CCCAGCGACG CCAGCAAACG 
CAGGAGACGG ACAACCGTTT CTTCTGGAAT CGCATGATGC ACATCCACCT GATGCGCTTC 
GGTATCGATT GCCAGTCCTG GTTGTTGCAA GCCATGTGCG GCTCCGTAGA AGTGCGCACC 
GTCTACATCG GTGCCAAACA GGCCCGTGCC GCCATCATTT CCCGACTGAG CTGCGAACGG 
GCTGGCACGC GTTTCAATGT CCGTGGTACC AACGATGAGG GCTATGTGGC TAACTTTGTG 
GAAACCGAGC AAGTGATCTA CGTGGACGGC GATGTTACTA GTTATGTGCA GACGCGAGGA 
TCGGTGCCAC TCTTCTGGGA ACAGCCAGGC GTCCAGGTTG GCTCGCACAA GGTGAAGCTA 
TCACGAGGAT TCGAGACATC GGCCGCCGCC TTTGATCGAC ACATGAGCAT GATGAGGCAA 
CGTTACGGCT ATCAGACGGT GGTGAATCTG CTAGGCAGCT CCCTTGTTGG CAGCAAGGAG 
GGCGAGGCCA TGCTGAGTAA TGAGTTCCAG CGTCATCACG GCATGTCAGC CCACAAGGAT 
GTGCCGCATG TGGTGTTTGA CTATCATCAG GAGTGTCGCG GCGGCAATTT CTCGGCGCTG 
GCCAAGCTCA AGGAACGGAT CGTAGCCTGC GGTGCTAACT ACGGCGTCTT CCACGCGTCC 
AATGGTCAGG TGTTGCGCGA GCAGTTCGGT GTTGTGCGCA CGAATTGCCT AGACTGTTTG 
GACAGGACAA ACTGTGTGCA GACGTATCTC GGGCTTGACA CGCTCGGTAT CCAGCTAGAG 
GCTTTGAAAA TGGGCGGCAA GCAGCAGAAT ATTTCGCGGT TTGAGGAGAT CTTCCGACAG 
ATGTGGATCA ACAACGGAAA TGAGGTCAGC AAGATCTACG CCGGCACCGG GGCCATCCAG 
GGGGGATCAA AACTAATGGA TGGTGCGCGA TCTGCAGCAA GAACAATTCA AAATAACCTA 
CTGGACAACT CAAAGCAGGA AGCCATTGAT GTCCTGCTAG TGGGCTCCAC GCTTAGCTCG 
GAGCTTGCGG ATCGGGCTCG CATCCTACTG CCCTCCAATA TGTTGCATGC ACCTACCACT 
GTGTTGAGAG AGCTATGCAA GCGCTACACT GAATATGTGC GTCCTCGAAT GGCACGTGTA 
GCCGTGGGTA CCTATAACGT CAACGGCGGC AAGCACTTCC GCAGCATTGT GTTCAAGGAT 
TCGCTGGCCG ATTGGCTGCT CGACTGCCAT GCCCTTGCCC GCTCCAAGGC GCTTGTAGAT 
GTGAACAATC CGTCGGAGAA CGTCGATCAT CCGGTGGATA TCTACGCCAT TGGATTCGAG 
GAGATTGTGG ATCTGAATGC TTCCAACATA ATGGCGGCCA GCACCGACAA TGCCAAGTTG 
TGGGCCGAGG AGCTGCAGAA AACGATCTCG CGGGACAATG ACTACGTGCT GCTCACATAC 
CAGCAACTGG TGGGCGTGTG CCTATACATC TACATCCGAC CGGAGCACGC GCCGCACATC 
CGGGACGTGG CCATCGACTG TGTTAAGACA GGATTGGGTG GTGCCACTGG GAATAAGGGT 
GCCTGTGCCA TTCGATTTGT GCTTCATGGT ACTTCCATGT GCTTCGTGTG TGCCCACTTT 
GCAGCCGGAC AGTCACAGGT GGCTGAAAGG AACGCTGACT ACGCGGAAAT CACCCGGAAG 
CTGGCCTTCC CGATGGGCAG GACGCTAAAA TCACACGACT GGGTGTTTTG GTGCGGCGAC 
TTCAACTATC GCATCGACAT GGAGAAGGAC GAATTAAAGG AGTGCGTACG TAATGGAGAT 
CTCTCAACCG TCCTCGAGTT CGATCAATTG CGCAAGGAGC AGGAGGCTGG CAATGTGTTT 
GGCGAATTCC TCGAGGGAGA GATCACTTTC GACCCGACGT ACAAGTATGA TTTGTTCAGC 
GACGACTACG ACACCTCGGA GAAACAGCGA GCGCCCGCCT GGACAGATCG GGTTCTCTGG 
CGTCGCAGGA AGGCGCTGGC CGAGGGCGAC TTTGCTGCCT CAGCCTGGAA TCCCGGCAAA 
TTGATTCACT ACGGTCGTTC GGAGCTAAAG CAGAGTGATC ATAGACCCGT GATCGCTATT 
ATTGATGCTG AGATAATGGA GATCGATCAG CAGCGGAGAC GTGCTGTATT CGAGCAGGTT 
ATCCGAGATC TGGGTCCGCC GGACTCCACG ATTGTGGTAC ATGTCCTGGA GTCTTCAGCA 
ACTGGAGATG AGGATGGACC AACCATATAC GACGAGAATG TGATGTCAGC CCTGATTACC 
GAGCTATCCA AGATGGGAGA GGTCACTTTG GTGCGCTATG TGGAGGACAC CATGTGGGTC 
ACATTTCGGG ATGGAGAATC GGCTTTAAAT GCGTCTTCTA AGAAGAGTAT CCAAGTATGC 
GGATTAGATC TTATCCTGGA GCTGAAGTCA AAGGACTGGC AGCATCTGGT GGACAGCGAG 
ATAGAGCTAT GCACCACGAA CACCATACCG CTGTGCGCTA ATCCTGTAGA GCATGCACAA 
CTCCTGCAGG CCATTACGCC GGAGTTGCCC CAGCGGCCCA AGCAGCCGCC CACACGTCCC 
CCAGCCCGTC CCCCAATGCC TATGTCGCCA AAGAACTCAC CACGCCACCT GCCCCACGTC 
GGGGTCATCA GCATTGTGCC CAAGCCGGCA AAGCCACCGA TGCCACCGCA ACCGCAATCT 
CAACCTCTAA TTCCGTCGCC GCTTCAGCCG CAGGTGGCGC CGCCTCGTCC GCCGGCAATG 
GACACCACGC CATCCTCCAA ATCGCAATCG CCAACGGAAC TTGTATCCGC TAGTTCTTCG 
ACGTCCTCTT CGGGAAAGAC TTCGCCCACA GCTCCAGCTG TGCTCTCCGG TCCGCCGACG 
CCGCCGCGCC AAATGCAGAG CAAGCAGGCC ACACCCGTCT CGACGCCCAC GCGCCAAGTC 
GGTTCGCCCA AGGATCCGAT TCCATCTGAT ACCGCCTACG AAACCGCCAG TAATATTTAC 
GAGGAGATTC AGGAGGATGT GCCTGCGCCA CGCCATCCGC CGCCGGCTGG ACCGCCGCCA 
GTGATAGGAG CACCAATGGG CCCGCCGCCA CCGCTGCCGA ATCGCAGGGG CCCACCACCC 
ATACCAAATA GGAGCGGCAA TGCTCCACCG CTGCCCACGC GACCCGCCAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj ( NM_130462.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569729.1 Drosophila melanogaster synaptojanin, transcript variant B (Synj), mRNA.

Price & Availability↑

CloneID	OFa10878
Clone ID Related Accession (Same CDS sequence)	NM_001259525.1 , NM_130462.4 , NM_166505.2 , NM_166505.1
Accession Version	NM_130462.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	synaptojanin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_130462.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATGT CCAAGGTGAT CCGTGTGCTG GAGAAGTCCA TTGCCCCCTC GCCGCACAGC 
GTATTACTGG AGCATCGGAA CAAGAGCGAC AGCATCCTGT TCGAGTCCCA TGCGGTGGCC 
CTGCTGACCC AGCAGGAGAC GGATGTCATC CGGAAGCAGT ACACCAAGGT CTGCGACGCC 
TACGGATGTC TGGGTGCCCT CCAACTAAAC GCCGGCGAGA GCACCGTGCT GTTCCTGGTG 
CTGGTCACCG GCTGTGTGTC CATGGGCAAG ATCGGCGACA TCGAGATCTT CCGGATCACA 
CAAACCACTT TTGTCTCGCT ACAAAATGCA GCGCCCAACG AAGACAAGAT CAGCGAGGTG 
CGCAAGCTGC TCAACTCGGG CACCTTCTAC TTTGCCCACA CCAATGCCAG TGCATCGGCA 
TCCGGAGCGT CATCGTATCG GTTCGATATT ACGCTTTGCG CCCAGCGACG CCAGCAAACG 
CAGGAGACGG ACAACCGTTT CTTCTGGAAT CGCATGATGC ACATCCACCT GATGCGCTTC 
GGTATCGATT GCCAGTCCTG GTTGTTGCAA GCCATGTGCG GCTCCGTAGA AGTGCGCACC 
GTCTACATCG GTGCCAAACA GGCCCGTGCC GCCATCATTT CCCGACTGAG CTGCGAACGG 
GCTGGCACGC GTTTCAATGT CCGTGGTACC AACGATGAGG GCTATGTGGC TAACTTTGTG 
GAAACCGAGC AAGTGATCTA CGTGGACGGC GATGTTACTA GTTATGTGCA GACGCGAGGA 
TCGGTGCCAC TCTTCTGGGA ACAGCCAGGC GTCCAGGTTG GCTCGCACAA GGTGAAGCTA 
TCACGAGGAT TCGAGACATC GGCCGCCGCC TTTGATCGAC ACATGAGCAT GATGAGGCAA 
CGTTACGGCT ATCAGACGGT GGTGAATCTG CTAGGCAGCT CCCTTGTTGG CAGCAAGGAG 
GGCGAGGCCA TGCTGAGTAA TGAGTTCCAG CGTCATCACG GCATGTCAGC CCACAAGGAT 
GTGCCGCATG TGGTGTTTGA CTATCATCAG GAGTGTCGCG GCGGCAATTT CTCGGCGCTG 
GCCAAGCTCA AGGAACGGAT CGTAGCCTGC GGTGCTAACT ACGGCGTCTT CCACGCGTCC 
AATGGTCAGG TGTTGCGCGA GCAGTTCGGT GTTGTGCGCA CGAATTGCCT AGACTGTTTG 
GACAGGACAA ACTGTGTGCA GACGTATCTC GGGCTTGACA CGCTCGGTAT CCAGCTAGAG 
GCTTTGAAAA TGGGCGGCAA GCAGCAGAAT ATTTCGCGGT TTGAGGAGAT CTTCCGACAG 
ATGTGGATCA ACAACGGAAA TGAGGTCAGC AAGATCTACG CCGGCACCGG GGCCATCCAG 
GGGGGATCAA AACTAATGGA TGGTGCGCGA TCTGCAGCAA GAACAATTCA AAATAACCTA 
CTGGACAACT CAAAGCAGGA AGCCATTGAT GTCCTGCTAG TGGGCTCCAC GCTTAGCTCG 
GAGCTTGCGG ATCGGGCTCG CATCCTACTG CCCTCCAATA TGTTGCATGC ACCTACCACT 
GTGTTGAGAG AGCTATGCAA GCGCTACACT GAATATGTGC GTCCTCGAAT GGCACGTGTA 
GCCGTGGGTA CCTATAACGT CAACGGCGGC AAGCACTTCC GCAGCATTGT GTTCAAGGAT 
TCGCTGGCCG ATTGGCTGCT CGACTGCCAT GCCCTTGCCC GCTCCAAGGC GCTTGTAGAT 
GTGAACAATC CGTCGGAGAA CGTCGATCAT CCGGTGGATA TCTACGCCAT TGGATTCGAG 
GAGATTGTGG ATCTGAATGC TTCCAACATA ATGGCGGCCA GCACCGACAA TGCCAAGTTG 
TGGGCCGAGG AGCTGCAGAA AACGATCTCG CGGGACAATG ACTACGTGCT GCTCACATAC 
CAGCAACTGG TGGGCGTGTG CCTATACATC TACATCCGAC CGGAGCACGC GCCGCACATC 
CGGGACGTGG CCATCGACTG TGTTAAGACA GGATTGGGTG GTGCCACTGG GAATAAGGGT 
GCCTGTGCCA TTCGATTTGT GCTTCATGGT ACTTCCATGT GCTTCGTGTG TGCCCACTTT 
GCAGCCGGAC AGTCACAGGT GGCTGAAAGG AACGCTGACT ACGCGGAAAT CACCCGGAAG 
CTGGCCTTCC CGATGGGCAG GACGCTAAAA TCACACGACT GGGTGTTTTG GTGCGGCGAC 
TTCAACTATC GCATCGACAT GGAGAAGGAC GAATTAAAGG AGTGCGTACG TAATGGAGAT 
CTCTCAACCG TCCTCGAGTT CGATCAATTG CGCAAGGAGC AGGAGGCTGG CAATGTGTTT 
GGCGAATTCC TCGAGGGAGA GATCACTTTC GACCCGACGT ACAAGTATGA TTTGTTCAGC 
GACGACTACG ACACCTCGGA GAAACAGCGA GCGCCCGCCT GGACAGATCG GGTTCTCTGG 
CGTCGCAGGA AGGCGCTGGC CGAGGGCGAC TTTGCTGCCT CAGCCTGGAA TCCCGGCAAA 
TTGATTCACT ACGGTCGTTC GGAGCTAAAG CAGAGTGATC ATAGACCCGT GATCGCTATT 
ATTGATGCTG AGATAATGGA GATCGATCAG CAGCGGAGAC GTGCTGTATT CGAGCAGGTT 
ATCCGAGATC TGGGTCCGCC GGACTCCACG ATTGTGGTAC ATGTCCTGGA GTCTTCAGCA 
ACTGGAGATG AGGATGGACC AACCATATAC GACGAGAATG TGATGTCAGC CCTGATTACC 
GAGCTATCCA AGATGGGAGA GGTCACTTTG GTGCGCTATG TGGAGGACAC CATGTGGGTC 
ACATTTCGGG ATGGAGAATC GGCTTTAAAT GCGTCTTCTA AGAAGAGTAT CCAAGTATGC 
GGATTAGATC TTATCCTGGA GCTGAAGTCA AAGGACTGGC AGCATCTGGT GGACAGCGAG 
ATAGAGCTAT GCACCACGAA CACCATACCG CTGTGCGCTA ATCCTGTAGA GCATGCACAA 
CTCCTGCAGG CCATTACGCC GGAGTTGCCC CAGCGGCCCA AGCAGCCGCC CACACGTCCC 
CCAGCCCGTC CCCCAATGCC TATGTCGCCA AAGAACTCAC CACGCCACCT GCCCCACGTC 
GGGGTCATCA GCATTGTGCC CAAGCCGGCA AAGCCACCGA TGCCACCGCA ACCGCAATCT 
CAACCTCTAA TTCCGTCGCC GCTTCAGCCG CAGGTGGCGC CGCCTCGTCC GCCGGCAATG 
GACACCACGC CATCCTCCAA ATCGCAATCG CCAACGGAAC TTGTATCCGC TAGTTCTTCG 
ACGTCCTCTT CGGGAAAGAC TTCGCCCACA GCTCCAGCTG TGCTCTCCGG TCCGCCGACG 
CCGCCGCGCC AAATGCAGAG CAAGCAGGCC ACACCCGTCT CGACGCCCAC GCGCCAAGTC 
GGTTCGCCCA AGGATCCGAT TCCATCTGAT ACCGCCTACG AAACCGCCAG TAATATTTAC 
GAGGAGATTC AGGAGGATGT GCCTGCGCCA CGCCATCCGC CGCCGGCTGG ACCGCCGCCA 
GTGATAGGAG CACCAATGGG CCCGCCGCCA CCGCTGCCGA ATCGCAGGGG CCCACCACCC 
ATACCAAATA GGAGCGGCAA TGCTCCACCG CTGCCCACGC GACCCGCCAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569729.1
CDS	136..3792
Translation	MAMSKVIRVLEKSIAPSPHSVLLEHRNKSDSILFESHAVALLTQQETDVIRKQYTKVCDAYGCLGALQLNAGESTVLFLVLVTGCVSMGKIGDIEIFRITQTTFVSLQNAAPNEDKISEVRKLLNSGTFYFAHTNASASASGASSYRFDITLCAQRRQQTQETDNRFFWNRMMHIHLMRFGIDCQSWLLQAMCGSVEVRTVYIGAKQARAAIISRLSCERAGTRFNVRGTNDEGYVANFVETEQVIYVDGDVTSYVQTRGSVPLFWEQPGVQVGSHKVKLSRGFETSAAAFDRHMSMMRQRYGYQTVVNLLGSSLVGSKEGEAMLSNEFQRHHGMSAHKDVPHVVFDYHQECRGGNFSALAKLKERIVACGANYGVFHASNGQVLREQFGVVRTNCLDCLDRTNCVQTYLGLDTLGIQLEALKMGGKQQNISRFEEIFRQMWINNGNEVSKIYAGTGAIQGGSKLMDGARSAARTIQNNLLDNSKQEAIDVLLVGSTLSSELADRARILLPSNMLHAPTTVLRELCKRYTEYVRPRMARVAVGTYNVNGGKHFRSIVFKDSLADWLLDCHALARSKALVDVNNPSENVDHPVDIYAIGFEEIVDLNASNIMAASTDNAKLWAEELQKTISRDNDYVLLTYQQLVGVCLYIYIRPEHAPHIRDVAIDCVKTGLGGATGNKGACAIRFVLHGTSMCFVCAHFAAGQSQVAERNADYAEITRKLAFPMGRTLKSHDWVFWCGDFNYRIDMEKDELKECVRNGDLSTVLEFDQLRKEQEAGNVFGEFLEGEITFDPTYKYDLFSDDYDTSEKQRAPAWTDRVLWRRRKALAEGDFAASAWNPGKLIHYGRSELKQSDHRPVIAIIDAEIMEIDQQRRRAVFEQVIRDLGPPDSTIVVHVLESSATGDEDGPTIYDENVMSALITELSKMGEVTLVRYVEDTMWVTFRDGESALNASSKKSIQVCGLDLILELKSKDWQHLVDSEIELCTTNTIPLCANPVEHAQLLQAITPELPQRPKQPPTRPPARPPMPMSPKNSPRHLPHVGVISIVPKPAKPPMPPQPQSQPLIPSPLQPQVAPPRPPAMDTTPSSKSQSPTELVSASSSTSSSGKTSPTAPAVLSGPPTPPRQMQSKQATPVSTPTRQVGSPKDPIPSDTAYETASNIYEEIQEDVPAPRHPPPAGPPPVIGAPMGPPPPLPNRRGPPPIPNRSGNAPPLPTRPANN

Target ORF information:

RefSeq Version	NM_130462.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster synaptojanin, transcript variant B (Synj), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130462.4

ORF Insert Sequence:

ATGGCCATGT CCAAGGTGAT CCGTGTGCTG GAGAAGTCCA TTGCCCCCTC GCCGCACAGC 
GTATTACTGG AGCATCGGAA CAAGAGCGAC AGCATCCTGT TCGAGTCCCA TGCGGTGGCC 
CTGCTGACCC AGCAGGAGAC GGATGTCATC CGGAAGCAGT ACACCAAGGT CTGCGACGCC 
TACGGATGTC TGGGTGCCCT CCAACTAAAC GCCGGCGAGA GCACCGTGCT GTTCCTGGTG 
CTGGTCACCG GCTGTGTGTC CATGGGCAAG ATCGGCGACA TCGAGATCTT CCGGATCACA 
CAAACCACTT TTGTCTCGCT ACAAAATGCA GCGCCCAACG AAGACAAGAT CAGCGAGGTG 
CGCAAGCTGC TCAACTCGGG CACCTTCTAC TTTGCCCACA CCAATGCCAG TGCATCGGCA 
TCCGGAGCGT CATCGTATCG GTTCGATATT ACGCTTTGCG CCCAGCGACG CCAGCAAACG 
CAGGAGACGG ACAACCGTTT CTTCTGGAAT CGCATGATGC ACATCCACCT GATGCGCTTC 
GGTATCGATT GCCAGTCCTG GTTGTTGCAA GCCATGTGCG GCTCCGTAGA AGTGCGCACC 
GTCTACATCG GTGCCAAACA GGCCCGTGCC GCCATCATTT CCCGACTGAG CTGCGAACGG 
GCTGGCACGC GTTTCAATGT CCGTGGTACC AACGATGAGG GCTATGTGGC TAACTTTGTG 
GAAACCGAGC AAGTGATCTA CGTGGACGGC GATGTTACTA GTTATGTGCA GACGCGAGGA 
TCGGTGCCAC TCTTCTGGGA ACAGCCAGGC GTCCAGGTTG GCTCGCACAA GGTGAAGCTA 
TCACGAGGAT TCGAGACATC GGCCGCCGCC TTTGATCGAC ACATGAGCAT GATGAGGCAA 
CGTTACGGCT ATCAGACGGT GGTGAATCTG CTAGGCAGCT CCCTTGTTGG CAGCAAGGAG 
GGCGAGGCCA TGCTGAGTAA TGAGTTCCAG CGTCATCACG GCATGTCAGC CCACAAGGAT 
GTGCCGCATG TGGTGTTTGA CTATCATCAG GAGTGTCGCG GCGGCAATTT CTCGGCGCTG 
GCCAAGCTCA AGGAACGGAT CGTAGCCTGC GGTGCTAACT ACGGCGTCTT CCACGCGTCC 
AATGGTCAGG TGTTGCGCGA GCAGTTCGGT GTTGTGCGCA CGAATTGCCT AGACTGTTTG 
GACAGGACAA ACTGTGTGCA GACGTATCTC GGGCTTGACA CGCTCGGTAT CCAGCTAGAG 
GCTTTGAAAA TGGGCGGCAA GCAGCAGAAT ATTTCGCGGT TTGAGGAGAT CTTCCGACAG 
ATGTGGATCA ACAACGGAAA TGAGGTCAGC AAGATCTACG CCGGCACCGG GGCCATCCAG 
GGGGGATCAA AACTAATGGA TGGTGCGCGA TCTGCAGCAA GAACAATTCA AAATAACCTA 
CTGGACAACT CAAAGCAGGA AGCCATTGAT GTCCTGCTAG TGGGCTCCAC GCTTAGCTCG 
GAGCTTGCGG ATCGGGCTCG CATCCTACTG CCCTCCAATA TGTTGCATGC ACCTACCACT 
GTGTTGAGAG AGCTATGCAA GCGCTACACT GAATATGTGC GTCCTCGAAT GGCACGTGTA 
GCCGTGGGTA CCTATAACGT CAACGGCGGC AAGCACTTCC GCAGCATTGT GTTCAAGGAT 
TCGCTGGCCG ATTGGCTGCT CGACTGCCAT GCCCTTGCCC GCTCCAAGGC GCTTGTAGAT 
GTGAACAATC CGTCGGAGAA CGTCGATCAT CCGGTGGATA TCTACGCCAT TGGATTCGAG 
GAGATTGTGG ATCTGAATGC TTCCAACATA ATGGCGGCCA GCACCGACAA TGCCAAGTTG 
TGGGCCGAGG AGCTGCAGAA AACGATCTCG CGGGACAATG ACTACGTGCT GCTCACATAC 
CAGCAACTGG TGGGCGTGTG CCTATACATC TACATCCGAC CGGAGCACGC GCCGCACATC 
CGGGACGTGG CCATCGACTG TGTTAAGACA GGATTGGGTG GTGCCACTGG GAATAAGGGT 
GCCTGTGCCA TTCGATTTGT GCTTCATGGT ACTTCCATGT GCTTCGTGTG TGCCCACTTT 
GCAGCCGGAC AGTCACAGGT GGCTGAAAGG AACGCTGACT ACGCGGAAAT CACCCGGAAG 
CTGGCCTTCC CGATGGGCAG GACGCTAAAA TCACACGACT GGGTGTTTTG GTGCGGCGAC 
TTCAACTATC GCATCGACAT GGAGAAGGAC GAATTAAAGG AGTGCGTACG TAATGGAGAT 
CTCTCAACCG TCCTCGAGTT CGATCAATTG CGCAAGGAGC AGGAGGCTGG CAATGTGTTT 
GGCGAATTCC TCGAGGGAGA GATCACTTTC GACCCGACGT ACAAGTATGA TTTGTTCAGC 
GACGACTACG ACACCTCGGA GAAACAGCGA GCGCCCGCCT GGACAGATCG GGTTCTCTGG 
CGTCGCAGGA AGGCGCTGGC CGAGGGCGAC TTTGCTGCCT CAGCCTGGAA TCCCGGCAAA 
TTGATTCACT ACGGTCGTTC GGAGCTAAAG CAGAGTGATC ATAGACCCGT GATCGCTATT 
ATTGATGCTG AGATAATGGA GATCGATCAG CAGCGGAGAC GTGCTGTATT CGAGCAGGTT 
ATCCGAGATC TGGGTCCGCC GGACTCCACG ATTGTGGTAC ATGTCCTGGA GTCTTCAGCA 
ACTGGAGATG AGGATGGACC AACCATATAC GACGAGAATG TGATGTCAGC CCTGATTACC 
GAGCTATCCA AGATGGGAGA GGTCACTTTG GTGCGCTATG TGGAGGACAC CATGTGGGTC 
ACATTTCGGG ATGGAGAATC GGCTTTAAAT GCGTCTTCTA AGAAGAGTAT CCAAGTATGC 
GGATTAGATC TTATCCTGGA GCTGAAGTCA AAGGACTGGC AGCATCTGGT GGACAGCGAG 
ATAGAGCTAT GCACCACGAA CACCATACCG CTGTGCGCTA ATCCTGTAGA GCATGCACAA 
CTCCTGCAGG CCATTACGCC GGAGTTGCCC CAGCGGCCCA AGCAGCCGCC CACACGTCCC 
CCAGCCCGTC CCCCAATGCC TATGTCGCCA AAGAACTCAC CACGCCACCT GCCCCACGTC 
GGGGTCATCA GCATTGTGCC CAAGCCGGCA AAGCCACCGA TGCCACCGCA ACCGCAATCT 
CAACCTCTAA TTCCGTCGCC GCTTCAGCCG CAGGTGGCGC CGCCTCGTCC GCCGGCAATG 
GACACCACGC CATCCTCCAA ATCGCAATCG CCAACGGAAC TTGTATCCGC TAGTTCTTCG 
ACGTCCTCTT CGGGAAAGAC TTCGCCCACA GCTCCAGCTG TGCTCTCCGG TCCGCCGACG 
CCGCCGCGCC AAATGCAGAG CAAGCAGGCC ACACCCGTCT CGACGCCCAC GCGCCAAGTC 
GGTTCGCCCA AGGATCCGAT TCCATCTGAT ACCGCCTACG AAACCGCCAG TAATATTTAC 
GAGGAGATTC AGGAGGATGT GCCTGCGCCA CGCCATCCGC CGCCGGCTGG ACCGCCGCCA 
GTGATAGGAG CACCAATGGG CCCGCCGCCA CCGCTGCCGA ATCGCAGGGG CCCACCACCC 
ATACCAAATA GGAGCGGCAA TGCTCCACCG CTGCCCACGC GACCCGCCAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj ( NM_166505.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726155.1 Drosophila melanogaster synaptojanin, transcript variant A (Synj), mRNA.

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CloneID	OFa10878
Clone ID Related Accession (Same CDS sequence)	NM_001259525.1 , NM_130462.4 , NM_166505.2 , NM_166505.1
Accession Version	NM_166505.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	synaptojanin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Apr 25, 2019 this sequence version replaced NM_166505.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATGT CCAAGGTGAT CCGTGTGCTG GAGAAGTCCA TTGCCCCCTC GCCGCACAGC 
GTATTACTGG AGCATCGGAA CAAGAGCGAC AGCATCCTGT TCGAGTCCCA TGCGGTGGCC 
CTGCTGACCC AGCAGGAGAC GGATGTCATC CGGAAGCAGT ACACCAAGGT CTGCGACGCC 
TACGGATGTC TGGGTGCCCT CCAACTAAAC GCCGGCGAGA GCACCGTGCT GTTCCTGGTG 
CTGGTCACCG GCTGTGTGTC CATGGGCAAG ATCGGCGACA TCGAGATCTT CCGGATCACA 
CAAACCACTT TTGTCTCGCT ACAAAATGCA GCGCCCAACG AAGACAAGAT CAGCGAGGTG 
CGCAAGCTGC TCAACTCGGG CACCTTCTAC TTTGCCCACA CCAATGCCAG TGCATCGGCA 
TCCGGAGCGT CATCGTATCG GTTCGATATT ACGCTTTGCG CCCAGCGACG CCAGCAAACG 
CAGGAGACGG ACAACCGTTT CTTCTGGAAT CGCATGATGC ACATCCACCT GATGCGCTTC 
GGTATCGATT GCCAGTCCTG GTTGTTGCAA GCCATGTGCG GCTCCGTAGA AGTGCGCACC 
GTCTACATCG GTGCCAAACA GGCCCGTGCC GCCATCATTT CCCGACTGAG CTGCGAACGG 
GCTGGCACGC GTTTCAATGT CCGTGGTACC AACGATGAGG GCTATGTGGC TAACTTTGTG 
GAAACCGAGC AAGTGATCTA CGTGGACGGC GATGTTACTA GTTATGTGCA GACGCGAGGA 
TCGGTGCCAC TCTTCTGGGA ACAGCCAGGC GTCCAGGTTG GCTCGCACAA GGTGAAGCTA 
TCACGAGGAT TCGAGACATC GGCCGCCGCC TTTGATCGAC ACATGAGCAT GATGAGGCAA 
CGTTACGGCT ATCAGACGGT GGTGAATCTG CTAGGCAGCT CCCTTGTTGG CAGCAAGGAG 
GGCGAGGCCA TGCTGAGTAA TGAGTTCCAG CGTCATCACG GCATGTCAGC CCACAAGGAT 
GTGCCGCATG TGGTGTTTGA CTATCATCAG GAGTGTCGCG GCGGCAATTT CTCGGCGCTG 
GCCAAGCTCA AGGAACGGAT CGTAGCCTGC GGTGCTAACT ACGGCGTCTT CCACGCGTCC 
AATGGTCAGG TGTTGCGCGA GCAGTTCGGT GTTGTGCGCA CGAATTGCCT AGACTGTTTG 
GACAGGACAA ACTGTGTGCA GACGTATCTC GGGCTTGACA CGCTCGGTAT CCAGCTAGAG 
GCTTTGAAAA TGGGCGGCAA GCAGCAGAAT ATTTCGCGGT TTGAGGAGAT CTTCCGACAG 
ATGTGGATCA ACAACGGAAA TGAGGTCAGC AAGATCTACG CCGGCACCGG GGCCATCCAG 
GGGGGATCAA AACTAATGGA TGGTGCGCGA TCTGCAGCAA GAACAATTCA AAATAACCTA 
CTGGACAACT CAAAGCAGGA AGCCATTGAT GTCCTGCTAG TGGGCTCCAC GCTTAGCTCG 
GAGCTTGCGG ATCGGGCTCG CATCCTACTG CCCTCCAATA TGTTGCATGC ACCTACCACT 
GTGTTGAGAG AGCTATGCAA GCGCTACACT GAATATGTGC GTCCTCGAAT GGCACGTGTA 
GCCGTGGGTA CCTATAACGT CAACGGCGGC AAGCACTTCC GCAGCATTGT GTTCAAGGAT 
TCGCTGGCCG ATTGGCTGCT CGACTGCCAT GCCCTTGCCC GCTCCAAGGC GCTTGTAGAT 
GTGAACAATC CGTCGGAGAA CGTCGATCAT CCGGTGGATA TCTACGCCAT TGGATTCGAG 
GAGATTGTGG ATCTGAATGC TTCCAACATA ATGGCGGCCA GCACCGACAA TGCCAAGTTG 
TGGGCCGAGG AGCTGCAGAA AACGATCTCG CGGGACAATG ACTACGTGCT GCTCACATAC 
CAGCAACTGG TGGGCGTGTG CCTATACATC TACATCCGAC CGGAGCACGC GCCGCACATC 
CGGGACGTGG CCATCGACTG TGTTAAGACA GGATTGGGTG GTGCCACTGG GAATAAGGGT 
GCCTGTGCCA TTCGATTTGT GCTTCATGGT ACTTCCATGT GCTTCGTGTG TGCCCACTTT 
GCAGCCGGAC AGTCACAGGT GGCTGAAAGG AACGCTGACT ACGCGGAAAT CACCCGGAAG 
CTGGCCTTCC CGATGGGCAG GACGCTAAAA TCACACGACT GGGTGTTTTG GTGCGGCGAC 
TTCAACTATC GCATCGACAT GGAGAAGGAC GAATTAAAGG AGTGCGTACG TAATGGAGAT 
CTCTCAACCG TCCTCGAGTT CGATCAATTG CGCAAGGAGC AGGAGGCTGG CAATGTGTTT 
GGCGAATTCC TCGAGGGAGA GATCACTTTC GACCCGACGT ACAAGTATGA TTTGTTCAGC 
GACGACTACG ACACCTCGGA GAAACAGCGA GCGCCCGCCT GGACAGATCG GGTTCTCTGG 
CGTCGCAGGA AGGCGCTGGC CGAGGGCGAC TTTGCTGCCT CAGCCTGGAA TCCCGGCAAA 
TTGATTCACT ACGGTCGTTC GGAGCTAAAG CAGAGTGATC ATAGACCCGT GATCGCTATT 
ATTGATGCTG AGATAATGGA GATCGATCAG CAGCGGAGAC GTGCTGTATT CGAGCAGGTT 
ATCCGAGATC TGGGTCCGCC GGACTCCACG ATTGTGGTAC ATGTCCTGGA GTCTTCAGCA 
ACTGGAGATG AGGATGGACC AACCATATAC GACGAGAATG TGATGTCAGC CCTGATTACC 
GAGCTATCCA AGATGGGAGA GGTCACTTTG GTGCGCTATG TGGAGGACAC CATGTGGGTC 
ACATTTCGGG ATGGAGAATC GGCTTTAAAT GCGTCTTCTA AGAAGAGTAT CCAAGTATGC 
GGATTAGATC TTATCCTGGA GCTGAAGTCA AAGGACTGGC AGCATCTGGT GGACAGCGAG 
ATAGAGCTAT GCACCACGAA CACCATACCG CTGTGCGCTA ATCCTGTAGA GCATGCACAA 
CTCCTGCAGG CCATTACGCC GGAGTTGCCC CAGCGGCCCA AGCAGCCGCC CACACGTCCC 
CCAGCCCGTC CCCCAATGCC TATGTCGCCA AAGAACTCAC CACGCCACCT GCCCCACGTC 
GGGGTCATCA GCATTGTGCC CAAGCCGGCA AAGCCACCGA TGCCACCGCA ACCGCAATCT 
CAACCTCTAA TTCCGTCGCC GCTTCAGCCG CAGGTGGCGC CGCCTCGTCC GCCGGCAATG 
GACACCACGC CATCCTCCAA ATCGCAATCG CCAACGGAAC TTGTATCCGC TAGTTCTTCG 
ACGTCCTCTT CGGGAAAGAC TTCGCCCACA GCTCCAGCTG TGCTCTCCGG TCCGCCGACG 
CCGCCGCGCC AAATGCAGAG CAAGCAGGCC ACACCCGTCT CGACGCCCAC GCGCCAAGTC 
GGTTCGCCCA AGGATCCGAT TCCATCTGAT ACCGCCTACG AAACCGCCAG TAATATTTAC 
GAGGAGATTC AGGAGGATGT GCCTGCGCCA CGCCATCCGC CGCCGGCTGG ACCGCCGCCA 
GTGATAGGAG CACCAATGGG CCCGCCGCCA CCGCTGCCGA ATCGCAGGGG CCCACCACCC 
ATACCAAATA GGAGCGGCAA TGCTCCACCG CTGCCCACGC GACCCGCCAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726155.1
CDS	202..3858
Translation	MAMSKVIRVLEKSIAPSPHSVLLEHRNKSDSILFESHAVALLTQQETDVIRKQYTKVCDAYGCLGALQLNAGESTVLFLVLVTGCVSMGKIGDIEIFRITQTTFVSLQNAAPNEDKISEVRKLLNSGTFYFAHTNASASASGASSYRFDITLCAQRRQQTQETDNRFFWNRMMHIHLMRFGIDCQSWLLQAMCGSVEVRTVYIGAKQARAAIISRLSCERAGTRFNVRGTNDEGYVANFVETEQVIYVDGDVTSYVQTRGSVPLFWEQPGVQVGSHKVKLSRGFETSAAAFDRHMSMMRQRYGYQTVVNLLGSSLVGSKEGEAMLSNEFQRHHGMSAHKDVPHVVFDYHQECRGGNFSALAKLKERIVACGANYGVFHASNGQVLREQFGVVRTNCLDCLDRTNCVQTYLGLDTLGIQLEALKMGGKQQNISRFEEIFRQMWINNGNEVSKIYAGTGAIQGGSKLMDGARSAARTIQNNLLDNSKQEAIDVLLVGSTLSSELADRARILLPSNMLHAPTTVLRELCKRYTEYVRPRMARVAVGTYNVNGGKHFRSIVFKDSLADWLLDCHALARSKALVDVNNPSENVDHPVDIYAIGFEEIVDLNASNIMAASTDNAKLWAEELQKTISRDNDYVLLTYQQLVGVCLYIYIRPEHAPHIRDVAIDCVKTGLGGATGNKGACAIRFVLHGTSMCFVCAHFAAGQSQVAERNADYAEITRKLAFPMGRTLKSHDWVFWCGDFNYRIDMEKDELKECVRNGDLSTVLEFDQLRKEQEAGNVFGEFLEGEITFDPTYKYDLFSDDYDTSEKQRAPAWTDRVLWRRRKALAEGDFAASAWNPGKLIHYGRSELKQSDHRPVIAIIDAEIMEIDQQRRRAVFEQVIRDLGPPDSTIVVHVLESSATGDEDGPTIYDENVMSALITELSKMGEVTLVRYVEDTMWVTFRDGESALNASSKKSIQVCGLDLILELKSKDWQHLVDSEIELCTTNTIPLCANPVEHAQLLQAITPELPQRPKQPPTRPPARPPMPMSPKNSPRHLPHVGVISIVPKPAKPPMPPQPQSQPLIPSPLQPQVAPPRPPAMDTTPSSKSQSPTELVSASSSTSSSGKTSPTAPAVLSGPPTPPRQMQSKQATPVSTPTRQVGSPKDPIPSDTAYETASNIYEEIQEDVPAPRHPPPAGPPPVIGAPMGPPPPLPNRRGPPPIPNRSGNAPPLPTRPANN

Target ORF information:

RefSeq Version	NM_166505.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster synaptojanin, transcript variant A (Synj), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166505.2

ORF Insert Sequence:

ATGGCCATGT CCAAGGTGAT CCGTGTGCTG GAGAAGTCCA TTGCCCCCTC GCCGCACAGC 
GTATTACTGG AGCATCGGAA CAAGAGCGAC AGCATCCTGT TCGAGTCCCA TGCGGTGGCC 
CTGCTGACCC AGCAGGAGAC GGATGTCATC CGGAAGCAGT ACACCAAGGT CTGCGACGCC 
TACGGATGTC TGGGTGCCCT CCAACTAAAC GCCGGCGAGA GCACCGTGCT GTTCCTGGTG 
CTGGTCACCG GCTGTGTGTC CATGGGCAAG ATCGGCGACA TCGAGATCTT CCGGATCACA 
CAAACCACTT TTGTCTCGCT ACAAAATGCA GCGCCCAACG AAGACAAGAT CAGCGAGGTG 
CGCAAGCTGC TCAACTCGGG CACCTTCTAC TTTGCCCACA CCAATGCCAG TGCATCGGCA 
TCCGGAGCGT CATCGTATCG GTTCGATATT ACGCTTTGCG CCCAGCGACG CCAGCAAACG 
CAGGAGACGG ACAACCGTTT CTTCTGGAAT CGCATGATGC ACATCCACCT GATGCGCTTC 
GGTATCGATT GCCAGTCCTG GTTGTTGCAA GCCATGTGCG GCTCCGTAGA AGTGCGCACC 
GTCTACATCG GTGCCAAACA GGCCCGTGCC GCCATCATTT CCCGACTGAG CTGCGAACGG 
GCTGGCACGC GTTTCAATGT CCGTGGTACC AACGATGAGG GCTATGTGGC TAACTTTGTG 
GAAACCGAGC AAGTGATCTA CGTGGACGGC GATGTTACTA GTTATGTGCA GACGCGAGGA 
TCGGTGCCAC TCTTCTGGGA ACAGCCAGGC GTCCAGGTTG GCTCGCACAA GGTGAAGCTA 
TCACGAGGAT TCGAGACATC GGCCGCCGCC TTTGATCGAC ACATGAGCAT GATGAGGCAA 
CGTTACGGCT ATCAGACGGT GGTGAATCTG CTAGGCAGCT CCCTTGTTGG CAGCAAGGAG 
GGCGAGGCCA TGCTGAGTAA TGAGTTCCAG CGTCATCACG GCATGTCAGC CCACAAGGAT 
GTGCCGCATG TGGTGTTTGA CTATCATCAG GAGTGTCGCG GCGGCAATTT CTCGGCGCTG 
GCCAAGCTCA AGGAACGGAT CGTAGCCTGC GGTGCTAACT ACGGCGTCTT CCACGCGTCC 
AATGGTCAGG TGTTGCGCGA GCAGTTCGGT GTTGTGCGCA CGAATTGCCT AGACTGTTTG 
GACAGGACAA ACTGTGTGCA GACGTATCTC GGGCTTGACA CGCTCGGTAT CCAGCTAGAG 
GCTTTGAAAA TGGGCGGCAA GCAGCAGAAT ATTTCGCGGT TTGAGGAGAT CTTCCGACAG 
ATGTGGATCA ACAACGGAAA TGAGGTCAGC AAGATCTACG CCGGCACCGG GGCCATCCAG 
GGGGGATCAA AACTAATGGA TGGTGCGCGA TCTGCAGCAA GAACAATTCA AAATAACCTA 
CTGGACAACT CAAAGCAGGA AGCCATTGAT GTCCTGCTAG TGGGCTCCAC GCTTAGCTCG 
GAGCTTGCGG ATCGGGCTCG CATCCTACTG CCCTCCAATA TGTTGCATGC ACCTACCACT 
GTGTTGAGAG AGCTATGCAA GCGCTACACT GAATATGTGC GTCCTCGAAT GGCACGTGTA 
GCCGTGGGTA CCTATAACGT CAACGGCGGC AAGCACTTCC GCAGCATTGT GTTCAAGGAT 
TCGCTGGCCG ATTGGCTGCT CGACTGCCAT GCCCTTGCCC GCTCCAAGGC GCTTGTAGAT 
GTGAACAATC CGTCGGAGAA CGTCGATCAT CCGGTGGATA TCTACGCCAT TGGATTCGAG 
GAGATTGTGG ATCTGAATGC TTCCAACATA ATGGCGGCCA GCACCGACAA TGCCAAGTTG 
TGGGCCGAGG AGCTGCAGAA AACGATCTCG CGGGACAATG ACTACGTGCT GCTCACATAC 
CAGCAACTGG TGGGCGTGTG CCTATACATC TACATCCGAC CGGAGCACGC GCCGCACATC 
CGGGACGTGG CCATCGACTG TGTTAAGACA GGATTGGGTG GTGCCACTGG GAATAAGGGT 
GCCTGTGCCA TTCGATTTGT GCTTCATGGT ACTTCCATGT GCTTCGTGTG TGCCCACTTT 
GCAGCCGGAC AGTCACAGGT GGCTGAAAGG AACGCTGACT ACGCGGAAAT CACCCGGAAG 
CTGGCCTTCC CGATGGGCAG GACGCTAAAA TCACACGACT GGGTGTTTTG GTGCGGCGAC 
TTCAACTATC GCATCGACAT GGAGAAGGAC GAATTAAAGG AGTGCGTACG TAATGGAGAT 
CTCTCAACCG TCCTCGAGTT CGATCAATTG CGCAAGGAGC AGGAGGCTGG CAATGTGTTT 
GGCGAATTCC TCGAGGGAGA GATCACTTTC GACCCGACGT ACAAGTATGA TTTGTTCAGC 
GACGACTACG ACACCTCGGA GAAACAGCGA GCGCCCGCCT GGACAGATCG GGTTCTCTGG 
CGTCGCAGGA AGGCGCTGGC CGAGGGCGAC TTTGCTGCCT CAGCCTGGAA TCCCGGCAAA 
TTGATTCACT ACGGTCGTTC GGAGCTAAAG CAGAGTGATC ATAGACCCGT GATCGCTATT 
ATTGATGCTG AGATAATGGA GATCGATCAG CAGCGGAGAC GTGCTGTATT CGAGCAGGTT 
ATCCGAGATC TGGGTCCGCC GGACTCCACG ATTGTGGTAC ATGTCCTGGA GTCTTCAGCA 
ACTGGAGATG AGGATGGACC AACCATATAC GACGAGAATG TGATGTCAGC CCTGATTACC 
GAGCTATCCA AGATGGGAGA GGTCACTTTG GTGCGCTATG TGGAGGACAC CATGTGGGTC 
ACATTTCGGG ATGGAGAATC GGCTTTAAAT GCGTCTTCTA AGAAGAGTAT CCAAGTATGC 
GGATTAGATC TTATCCTGGA GCTGAAGTCA AAGGACTGGC AGCATCTGGT GGACAGCGAG 
ATAGAGCTAT GCACCACGAA CACCATACCG CTGTGCGCTA ATCCTGTAGA GCATGCACAA 
CTCCTGCAGG CCATTACGCC GGAGTTGCCC CAGCGGCCCA AGCAGCCGCC CACACGTCCC 
CCAGCCCGTC CCCCAATGCC TATGTCGCCA AAGAACTCAC CACGCCACCT GCCCCACGTC 
GGGGTCATCA GCATTGTGCC CAAGCCGGCA AAGCCACCGA TGCCACCGCA ACCGCAATCT 
CAACCTCTAA TTCCGTCGCC GCTTCAGCCG CAGGTGGCGC CGCCTCGTCC GCCGGCAATG 
GACACCACGC CATCCTCCAA ATCGCAATCG CCAACGGAAC TTGTATCCGC TAGTTCTTCG 
ACGTCCTCTT CGGGAAAGAC TTCGCCCACA GCTCCAGCTG TGCTCTCCGG TCCGCCGACG 
CCGCCGCGCC AAATGCAGAG CAAGCAGGCC ACACCCGTCT CGACGCCCAC GCGCCAAGTC 
GGTTCGCCCA AGGATCCGAT TCCATCTGAT ACCGCCTACG AAACCGCCAG TAATATTTAC 
GAGGAGATTC AGGAGGATGT GCCTGCGCCA CGCCATCCGC CGCCGGCTGG ACCGCCGCCA 
GTGATAGGAG CACCAATGGG CCCGCCGCCA CCGCTGCCGA ATCGCAGGGG CCCACCACCC 
ATACCAAATA GGAGCGGCAA TGCTCCACCG CTGCCCACGC GACCCGCCAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj ( NM_166505.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726155.1 Drosophila melanogaster synaptojanin, transcript variant A (Synj), mRNA.

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CloneID	OFa10878
Clone ID Related Accession (Same CDS sequence)	NM_001259525.1 , NM_130462.4 , NM_166505.2 , NM_166505.1
Accession Version	NM_166505.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-15
Organism	Drosophila melanogaster(fruit fly)
Product	synaptojanin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.18 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATGT CCAAGGTGAT CCGTGTGCTG GAGAAGTCCA TTGCCCCCTC GCCGCACAGC 
GTATTACTGG AGCATCGGAA CAAGAGCGAC AGCATCCTGT TCGAGTCCCA TGCGGTGGCC 
CTGCTGACCC AGCAGGAGAC GGATGTCATC CGGAAGCAGT ACACCAAGGT CTGCGACGCC 
TACGGATGTC TGGGTGCCCT CCAACTAAAC GCCGGCGAGA GCACCGTGCT GTTCCTGGTG 
CTGGTCACCG GCTGTGTGTC CATGGGCAAG ATCGGCGACA TCGAGATCTT CCGGATCACA 
CAAACCACTT TTGTCTCGCT ACAAAATGCA GCGCCCAACG AAGACAAGAT CAGCGAGGTG 
CGCAAGCTGC TCAACTCGGG CACCTTCTAC TTTGCCCACA CCAATGCCAG TGCATCGGCA 
TCCGGAGCGT CATCGTATCG GTTCGATATT ACGCTTTGCG CCCAGCGACG CCAGCAAACG 
CAGGAGACGG ACAACCGTTT CTTCTGGAAT CGCATGATGC ACATCCACCT GATGCGCTTC 
GGTATCGATT GCCAGTCCTG GTTGTTGCAA GCCATGTGCG GCTCCGTAGA AGTGCGCACC 
GTCTACATCG GTGCCAAACA GGCCCGTGCC GCCATCATTT CCCGACTGAG CTGCGAACGG 
GCTGGCACGC GTTTCAATGT CCGTGGTACC AACGATGAGG GCTATGTGGC TAACTTTGTG 
GAAACCGAGC AAGTGATCTA CGTGGACGGC GATGTTACTA GTTATGTGCA GACGCGAGGA 
TCGGTGCCAC TCTTCTGGGA ACAGCCAGGC GTCCAGGTTG GCTCGCACAA GGTGAAGCTA 
TCACGAGGAT TCGAGACATC GGCCGCCGCC TTTGATCGAC ACATGAGCAT GATGAGGCAA 
CGTTACGGCT ATCAGACGGT GGTGAATCTG CTAGGCAGCT CCCTTGTTGG CAGCAAGGAG 
GGCGAGGCCA TGCTGAGTAA TGAGTTCCAG CGTCATCACG GCATGTCAGC CCACAAGGAT 
GTGCCGCATG TGGTGTTTGA CTATCATCAG GAGTGTCGCG GCGGCAATTT CTCGGCGCTG 
GCCAAGCTCA AGGAACGGAT CGTAGCCTGC GGTGCTAACT ACGGCGTCTT CCACGCGTCC 
AATGGTCAGG TGTTGCGCGA GCAGTTCGGT GTTGTGCGCA CGAATTGCCT AGACTGTTTG 
GACAGGACAA ACTGTGTGCA GACGTATCTC GGGCTTGACA CGCTCGGTAT CCAGCTAGAG 
GCTTTGAAAA TGGGCGGCAA GCAGCAGAAT ATTTCGCGGT TTGAGGAGAT CTTCCGACAG 
ATGTGGATCA ACAACGGAAA TGAGGTCAGC AAGATCTACG CCGGCACCGG GGCCATCCAG 
GGGGGATCAA AACTAATGGA TGGTGCGCGA TCTGCAGCAA GAACAATTCA AAATAACCTA 
CTGGACAACT CAAAGCAGGA AGCCATTGAT GTCCTGCTAG TGGGCTCCAC GCTTAGCTCG 
GAGCTTGCGG ATCGGGCTCG CATCCTACTG CCCTCCAATA TGTTGCATGC ACCTACCACT 
GTGTTGAGAG AGCTATGCAA GCGCTACACT GAATATGTGC GTCCTCGAAT GGCACGTGTA 
GCCGTGGGTA CCTATAACGT CAACGGCGGC AAGCACTTCC GCAGCATTGT GTTCAAGGAT 
TCGCTGGCCG ATTGGCTGCT CGACTGCCAT GCCCTTGCCC GCTCCAAGGC GCTTGTAGAT 
GTGAACAATC CGTCGGAGAA CGTCGATCAT CCGGTGGATA TCTACGCCAT TGGATTCGAG 
GAGATTGTGG ATCTGAATGC TTCCAACATA ATGGCGGCCA GCACCGACAA TGCCAAGTTG 
TGGGCCGAGG AGCTGCAGAA AACGATCTCG CGGGACAATG ACTACGTGCT GCTCACATAC 
CAGCAACTGG TGGGCGTGTG CCTATACATC TACATCCGAC CGGAGCACGC GCCGCACATC 
CGGGACGTGG CCATCGACTG TGTTAAGACA GGATTGGGTG GTGCCACTGG GAATAAGGGT 
GCCTGTGCCA TTCGATTTGT GCTTCATGGT ACTTCCATGT GCTTCGTGTG TGCCCACTTT 
GCAGCCGGAC AGTCACAGGT GGCTGAAAGG AACGCTGACT ACGCGGAAAT CACCCGGAAG 
CTGGCCTTCC CGATGGGCAG GACGCTAAAA TCACACGACT GGGTGTTTTG GTGCGGCGAC 
TTCAACTATC GCATCGACAT GGAGAAGGAC GAATTAAAGG AGTGCGTACG TAATGGAGAT 
CTCTCAACCG TCCTCGAGTT CGATCAATTG CGCAAGGAGC AGGAGGCTGG CAATGTGTTT 
GGCGAATTCC TCGAGGGAGA GATCACTTTC GACCCGACGT ACAAGTATGA TTTGTTCAGC 
GACGACTACG ACACCTCGGA GAAACAGCGA GCGCCCGCCT GGACAGATCG GGTTCTCTGG 
CGTCGCAGGA AGGCGCTGGC CGAGGGCGAC TTTGCTGCCT CAGCCTGGAA TCCCGGCAAA 
TTGATTCACT ACGGTCGTTC GGAGCTAAAG CAGAGTGATC ATAGACCCGT GATCGCTATT 
ATTGATGCTG AGATAATGGA GATCGATCAG CAGCGGAGAC GTGCTGTATT CGAGCAGGTT 
ATCCGAGATC TGGGTCCGCC GGACTCCACG ATTGTGGTAC ATGTCCTGGA GTCTTCAGCA 
ACTGGAGATG AGGATGGACC AACCATATAC GACGAGAATG TGATGTCAGC CCTGATTACC 
GAGCTATCCA AGATGGGAGA GGTCACTTTG GTGCGCTATG TGGAGGACAC CATGTGGGTC 
ACATTTCGGG ATGGAGAATC GGCTTTAAAT GCGTCTTCTA AGAAGAGTAT CCAAGTATGC 
GGATTAGATC TTATCCTGGA GCTGAAGTCA AAGGACTGGC AGCATCTGGT GGACAGCGAG 
ATAGAGCTAT GCACCACGAA CACCATACCG CTGTGCGCTA ATCCTGTAGA GCATGCACAA 
CTCCTGCAGG CCATTACGCC GGAGTTGCCC CAGCGGCCCA AGCAGCCGCC CACACGTCCC 
CCAGCCCGTC CCCCAATGCC TATGTCGCCA AAGAACTCAC CACGCCACCT GCCCCACGTC 
GGGGTCATCA GCATTGTGCC CAAGCCGGCA AAGCCACCGA TGCCACCGCA ACCGCAATCT 
CAACCTCTAA TTCCGTCGCC GCTTCAGCCG CAGGTGGCGC CGCCTCGTCC GCCGGCAATG 
GACACCACGC CATCCTCCAA ATCGCAATCG CCAACGGAAC TTGTATCCGC TAGTTCTTCG 
ACGTCCTCTT CGGGAAAGAC TTCGCCCACA GCTCCAGCTG TGCTCTCCGG TCCGCCGACG 
CCGCCGCGCC AAATGCAGAG CAAGCAGGCC ACACCCGTCT CGACGCCCAC GCGCCAAGTC 
GGTTCGCCCA AGGATCCGAT TCCATCTGAT ACCGCCTACG AAACCGCCAG TAATATTTAC 
GAGGAGATTC AGGAGGATGT GCCTGCGCCA CGCCATCCGC CGCCGGCTGG ACCGCCGCCA 
GTGATAGGAG CACCAATGGG CCCGCCGCCA CCGCTGCCGA ATCGCAGGGG CCCACCACCC 
ATACCAAATA GGAGCGGCAA TGCTCCACCG CTGCCCACGC GACCCGCCAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726155.1
CDS	202..3858
Translation	MAMSKVIRVLEKSIAPSPHSVLLEHRNKSDSILFESHAVALLTQQETDVIRKQYTKVCDAYGCLGALQLNAGESTVLFLVLVTGCVSMGKIGDIEIFRITQTTFVSLQNAAPNEDKISEVRKLLNSGTFYFAHTNASASASGASSYRFDITLCAQRRQQTQETDNRFFWNRMMHIHLMRFGIDCQSWLLQAMCGSVEVRTVYIGAKQARAAIISRLSCERAGTRFNVRGTNDEGYVANFVETEQVIYVDGDVTSYVQTRGSVPLFWEQPGVQVGSHKVKLSRGFETSAAAFDRHMSMMRQRYGYQTVVNLLGSSLVGSKEGEAMLSNEFQRHHGMSAHKDVPHVVFDYHQECRGGNFSALAKLKERIVACGANYGVFHASNGQVLREQFGVVRTNCLDCLDRTNCVQTYLGLDTLGIQLEALKMGGKQQNISRFEEIFRQMWINNGNEVSKIYAGTGAIQGGSKLMDGARSAARTIQNNLLDNSKQEAIDVLLVGSTLSSELADRARILLPSNMLHAPTTVLRELCKRYTEYVRPRMARVAVGTYNVNGGKHFRSIVFKDSLADWLLDCHALARSKALVDVNNPSENVDHPVDIYAIGFEEIVDLNASNIMAASTDNAKLWAEELQKTISRDNDYVLLTYQQLVGVCLYIYIRPEHAPHIRDVAIDCVKTGLGGATGNKGACAIRFVLHGTSMCFVCAHFAAGQSQVAERNADYAEITRKLAFPMGRTLKSHDWVFWCGDFNYRIDMEKDELKECVRNGDLSTVLEFDQLRKEQEAGNVFGEFLEGEITFDPTYKYDLFSDDYDTSEKQRAPAWTDRVLWRRRKALAEGDFAASAWNPGKLIHYGRSELKQSDHRPVIAIIDAEIMEIDQQRRRAVFEQVIRDLGPPDSTIVVHVLESSATGDEDGPTIYDENVMSALITELSKMGEVTLVRYVEDTMWVTFRDGESALNASSKKSIQVCGLDLILELKSKDWQHLVDSEIELCTTNTIPLCANPVEHAQLLQAITPELPQRPKQPPTRPPARPPMPMSPKNSPRHLPHVGVISIVPKPAKPPMPPQPQSQPLIPSPLQPQVAPPRPPAMDTTPSSKSQSPTELVSASSSTSSSGKTSPTAPAVLSGPPTPPRQMQSKQATPVSTPTRQVGSPKDPIPSDTAYETASNIYEEIQEDVPAPRHPPPAGPPPVIGAPMGPPPPLPNRRGPPPIPNRSGNAPPLPTRPANN

Target ORF information:

RefSeq Version	NM_166505.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster synaptojanin, transcript variant A (Synj), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166505.1

ORF Insert Sequence:

ATGGCCATGT CCAAGGTGAT CCGTGTGCTG GAGAAGTCCA TTGCCCCCTC GCCGCACAGC 
GTATTACTGG AGCATCGGAA CAAGAGCGAC AGCATCCTGT TCGAGTCCCA TGCGGTGGCC 
CTGCTGACCC AGCAGGAGAC GGATGTCATC CGGAAGCAGT ACACCAAGGT CTGCGACGCC 
TACGGATGTC TGGGTGCCCT CCAACTAAAC GCCGGCGAGA GCACCGTGCT GTTCCTGGTG 
CTGGTCACCG GCTGTGTGTC CATGGGCAAG ATCGGCGACA TCGAGATCTT CCGGATCACA 
CAAACCACTT TTGTCTCGCT ACAAAATGCA GCGCCCAACG AAGACAAGAT CAGCGAGGTG 
CGCAAGCTGC TCAACTCGGG CACCTTCTAC TTTGCCCACA CCAATGCCAG TGCATCGGCA 
TCCGGAGCGT CATCGTATCG GTTCGATATT ACGCTTTGCG CCCAGCGACG CCAGCAAACG 
CAGGAGACGG ACAACCGTTT CTTCTGGAAT CGCATGATGC ACATCCACCT GATGCGCTTC 
GGTATCGATT GCCAGTCCTG GTTGTTGCAA GCCATGTGCG GCTCCGTAGA AGTGCGCACC 
GTCTACATCG GTGCCAAACA GGCCCGTGCC GCCATCATTT CCCGACTGAG CTGCGAACGG 
GCTGGCACGC GTTTCAATGT CCGTGGTACC AACGATGAGG GCTATGTGGC TAACTTTGTG 
GAAACCGAGC AAGTGATCTA CGTGGACGGC GATGTTACTA GTTATGTGCA GACGCGAGGA 
TCGGTGCCAC TCTTCTGGGA ACAGCCAGGC GTCCAGGTTG GCTCGCACAA GGTGAAGCTA 
TCACGAGGAT TCGAGACATC GGCCGCCGCC TTTGATCGAC ACATGAGCAT GATGAGGCAA 
CGTTACGGCT ATCAGACGGT GGTGAATCTG CTAGGCAGCT CCCTTGTTGG CAGCAAGGAG 
GGCGAGGCCA TGCTGAGTAA TGAGTTCCAG CGTCATCACG GCATGTCAGC CCACAAGGAT 
GTGCCGCATG TGGTGTTTGA CTATCATCAG GAGTGTCGCG GCGGCAATTT CTCGGCGCTG 
GCCAAGCTCA AGGAACGGAT CGTAGCCTGC GGTGCTAACT ACGGCGTCTT CCACGCGTCC 
AATGGTCAGG TGTTGCGCGA GCAGTTCGGT GTTGTGCGCA CGAATTGCCT AGACTGTTTG 
GACAGGACAA ACTGTGTGCA GACGTATCTC GGGCTTGACA CGCTCGGTAT CCAGCTAGAG 
GCTTTGAAAA TGGGCGGCAA GCAGCAGAAT ATTTCGCGGT TTGAGGAGAT CTTCCGACAG 
ATGTGGATCA ACAACGGAAA TGAGGTCAGC AAGATCTACG CCGGCACCGG GGCCATCCAG 
GGGGGATCAA AACTAATGGA TGGTGCGCGA TCTGCAGCAA GAACAATTCA AAATAACCTA 
CTGGACAACT CAAAGCAGGA AGCCATTGAT GTCCTGCTAG TGGGCTCCAC GCTTAGCTCG 
GAGCTTGCGG ATCGGGCTCG CATCCTACTG CCCTCCAATA TGTTGCATGC ACCTACCACT 
GTGTTGAGAG AGCTATGCAA GCGCTACACT GAATATGTGC GTCCTCGAAT GGCACGTGTA 
GCCGTGGGTA CCTATAACGT CAACGGCGGC AAGCACTTCC GCAGCATTGT GTTCAAGGAT 
TCGCTGGCCG ATTGGCTGCT CGACTGCCAT GCCCTTGCCC GCTCCAAGGC GCTTGTAGAT 
GTGAACAATC CGTCGGAGAA CGTCGATCAT CCGGTGGATA TCTACGCCAT TGGATTCGAG 
GAGATTGTGG ATCTGAATGC TTCCAACATA ATGGCGGCCA GCACCGACAA TGCCAAGTTG 
TGGGCCGAGG AGCTGCAGAA AACGATCTCG CGGGACAATG ACTACGTGCT GCTCACATAC 
CAGCAACTGG TGGGCGTGTG CCTATACATC TACATCCGAC CGGAGCACGC GCCGCACATC 
CGGGACGTGG CCATCGACTG TGTTAAGACA GGATTGGGTG GTGCCACTGG GAATAAGGGT 
GCCTGTGCCA TTCGATTTGT GCTTCATGGT ACTTCCATGT GCTTCGTGTG TGCCCACTTT 
GCAGCCGGAC AGTCACAGGT GGCTGAAAGG AACGCTGACT ACGCGGAAAT CACCCGGAAG 
CTGGCCTTCC CGATGGGCAG GACGCTAAAA TCACACGACT GGGTGTTTTG GTGCGGCGAC 
TTCAACTATC GCATCGACAT GGAGAAGGAC GAATTAAAGG AGTGCGTACG TAATGGAGAT 
CTCTCAACCG TCCTCGAGTT CGATCAATTG CGCAAGGAGC AGGAGGCTGG CAATGTGTTT 
GGCGAATTCC TCGAGGGAGA GATCACTTTC GACCCGACGT ACAAGTATGA TTTGTTCAGC 
GACGACTACG ACACCTCGGA GAAACAGCGA GCGCCCGCCT GGACAGATCG GGTTCTCTGG 
CGTCGCAGGA AGGCGCTGGC CGAGGGCGAC TTTGCTGCCT CAGCCTGGAA TCCCGGCAAA 
TTGATTCACT ACGGTCGTTC GGAGCTAAAG CAGAGTGATC ATAGACCCGT GATCGCTATT 
ATTGATGCTG AGATAATGGA GATCGATCAG CAGCGGAGAC GTGCTGTATT CGAGCAGGTT 
ATCCGAGATC TGGGTCCGCC GGACTCCACG ATTGTGGTAC ATGTCCTGGA GTCTTCAGCA 
ACTGGAGATG AGGATGGACC AACCATATAC GACGAGAATG TGATGTCAGC CCTGATTACC 
GAGCTATCCA AGATGGGAGA GGTCACTTTG GTGCGCTATG TGGAGGACAC CATGTGGGTC 
ACATTTCGGG ATGGAGAATC GGCTTTAAAT GCGTCTTCTA AGAAGAGTAT CCAAGTATGC 
GGATTAGATC TTATCCTGGA GCTGAAGTCA AAGGACTGGC AGCATCTGGT GGACAGCGAG 
ATAGAGCTAT GCACCACGAA CACCATACCG CTGTGCGCTA ATCCTGTAGA GCATGCACAA 
CTCCTGCAGG CCATTACGCC GGAGTTGCCC CAGCGGCCCA AGCAGCCGCC CACACGTCCC 
CCAGCCCGTC CCCCAATGCC TATGTCGCCA AAGAACTCAC CACGCCACCT GCCCCACGTC 
GGGGTCATCA GCATTGTGCC CAAGCCGGCA AAGCCACCGA TGCCACCGCA ACCGCAATCT 
CAACCTCTAA TTCCGTCGCC GCTTCAGCCG CAGGTGGCGC CGCCTCGTCC GCCGGCAATG 
GACACCACGC CATCCTCCAA ATCGCAATCG CCAACGGAAC TTGTATCCGC TAGTTCTTCG 
ACGTCCTCTT CGGGAAAGAC TTCGCCCACA GCTCCAGCTG TGCTCTCCGG TCCGCCGACG 
CCGCCGCGCC AAATGCAGAG CAAGCAGGCC ACACCCGTCT CGACGCCCAC GCGCCAAGTC 
GGTTCGCCCA AGGATCCGAT TCCATCTGAT ACCGCCTACG AAACCGCCAG TAATATTTAC 
GAGGAGATTC AGGAGGATGT GCCTGCGCCA CGCCATCCGC CGCCGGCTGG ACCGCCGCCA 
GTGATAGGAG CACCAATGGG CCCGCCGCCA CCGCTGCCGA ATCGCAGGGG CCCACCACCC 
ATACCAAATA GGAGCGGCAA TGCTCCACCG CTGCCCACGC GACCCGCCAA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Synj cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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