Synj1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Synj1
Entrez Gene ID	104015
Full Name	synaptojanin 1
Synonyms	A930006D20Rik,AA675315,mKIAA0910
General protein information	Preferred Names synaptojanin 1 Names synaptojanin-1 synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 C3.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_011246080.2	XP_011244382.1	synaptojanin-1 isoform X7
XM_011246081.2	XP_011244383.1	synaptojanin-1 isoform X9
XM_011246085.2	XP_011244387.1	synaptojanin-1 isoform X14
XM_006522846.2	XP_006522909.1	synaptojanin-1 isoform X22
XM_011246078.3	XP_011244380.1	synaptojanin-1 isoform X5
XM_030248903.1	XP_030104763.1	synaptojanin-1 isoform X9
XM_017316836.2	XP_017172325.1	synaptojanin-1 isoform X10
XM_011246082.3	XP_011244384.1	synaptojanin-1 isoform X12
XM_017316837.2	XP_017172326.1	synaptojanin-1 isoform X18
XM_011246088.3	XP_011244390.1	synaptojanin-1 isoform X21
XM_030248909.1	XP_030104769.1	synaptojanin-1 isoform X25
XM_030248910.1	XP_030104770.1	synaptojanin-1 isoform X27
XM_006522846.3	XP_006522909.1	synaptojanin-1 isoform X31
XM_011246075.2	XP_011244377.1	synaptojanin-1 isoform X1
XM_006522837.3	XP_006522900.1	synaptojanin-1 isoform X5
XM_011246087.2	XP_011244389.1	synaptojanin-1 isoform X15
XM_017316838.1	XP_017172327.1	synaptojanin-1 isoform X17
XM_017316841.1	XP_017172330.1	synaptojanin-1 isoform X20
XM_011246081.3	XP_011244383.1	synaptojanin-1 isoform X11
XM_011246089.3	XP_011244391.1	synaptojanin-1 isoform X30
XM_011246076.2	XP_011244378.1	synaptojanin-1 isoform X2
XM_011246077.2	XP_011244379.1	synaptojanin-1 isoform X3
XM_011246089.2	XP_011244391.1	synaptojanin-1 isoform X21
XM_011246076.3	XP_011244378.1	synaptojanin-1 isoform X2
XM_030248902.1	XP_030104762.1	synaptojanin-1 isoform X3
XM_011246077.3	XP_011244379.1	synaptojanin-1 isoform X4
XM_011246079.3	XP_011244381.1	synaptojanin-1 isoform X7
XM_030248904.1	XP_030104764.1	synaptojanin-1 isoform X13
XM_030248906.1	XP_030104766.1	synaptojanin-1 isoform X17
XM_011246085.3	XP_011244387.1	synaptojanin-1 isoform X19
XM_030248908.1	XP_030104768.1	synaptojanin-1 isoform X24
XM_017316839.2	XP_017172328.1	synaptojanin-1 isoform X26
XM_017316841.2	XP_017172330.1	synaptojanin-1 isoform X29
NM_001164483.1	NP_001157955.1	synaptojanin-1 isoform a
XM_011246078.2	XP_011244380.1	synaptojanin-1 isoform X4
XM_011246079.2	XP_011244381.1	synaptojanin-1 isoform X6
XM_011246082.2	XP_011244384.1	synaptojanin-1 isoform X10
XM_017316840.1	XP_017172329.1	synaptojanin-1 isoform X19
XM_011246083.1	XP_011244385.1	synaptojanin-1 isoform X14
XM_011246084.1	XP_011244386.1	synaptojanin-1 isoform X16
XM_017316840.2	XP_017172329.1	synaptojanin-1 isoform X28
XM_017316836.1	XP_017172325.1	synaptojanin-1 isoform X8
XM_017316837.1	XP_017172326.1	synaptojanin-1 isoform X13
XM_011246088.2	XP_011244390.1	synaptojanin-1 isoform X16
XM_017316839.1	XP_017172328.1	synaptojanin-1 isoform X18
XM_011246075.3	XP_011244377.1	synaptojanin-1 isoform X1
XM_006522837.4	XP_006522900.1	synaptojanin-1 isoform X6
XM_011246080.3	XP_011244382.1	synaptojanin-1 isoform X8
XM_030248905.1	XP_030104765.1	synaptojanin-1 isoform X15
XM_011246087.3	XP_011244389.1	synaptojanin-1 isoform X20
XM_030248907.1	XP_030104767.1	synaptojanin-1 isoform X22
XM_017316838.2	XP_017172327.1	synaptojanin-1 isoform X23
NM_001045515.1	NP_001038980.1	synaptojanin-1 isoform b

Pathways from BioSystems

Homologies

Homo sapiens (human)	SYNJ1	NP_003886.3
Pan troglodytes (chimpanzee)	SYNJ1	XP_531429.2
Gallus gallus (chicken)	SYNJ1	XP_416706.3
Xenopus tropicalis (tropical clawed frog)	synj1	XP_004912203.1
Canis lupus familiaris (dog)	SYNJ1	XP_005638893.1
Caenorhabditis elegans (roundworm)	unc-26	NP_001023266.1
Rattus norvegicus (Norway rat)	Synj1	NP_445928.2
Danio rerio (zebrafish)	synj1	NP_001007031.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012175	XP_320360.4
Caenorhabditis elegans (roundworm)	unc-26	NP_001129862.1
	SYNJ1	XP_002803210.1
Bos taurus (cattle)	SYNJ1	NP_776893.2
Mus musculus (house mouse)	Synj1	NP_001157955.1
Drosophila melanogaster (fruit fly)	synj	NP_001246454.1

Gene Ontology
Bibliography

Related articles in PubMed

Parkinson's Disease-Associated LRRK2 Hyperactive Kinase Mutant Disrupts Synaptic Vesicle Trafficking in Ventral Midbrain Neurons.
Pan PY, Li X, Wang J, Powell J, Wang Q, Zhang Y, Chen Z, Wicinski B, Hof P, Ryan TA, Yue Z
The Journal of neuroscience : the official journal of the Society for Neuroscience37(47)11366-11376(2017 Nov)

ApoE4-associated phospholipid dysregulation contributes to development of Tau hyper-phosphorylation after traumatic brain injury.
Cao J, Gaamouch FE, Meabon JS, Meeker KD, Zhu L, Zhong MB, Bendik J, Elder G, Jing P, Xia J, Luo W, Cook DG, Cai D
Scientific reports7(1)11372(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Parkinson Sac Domain Mutation in Synaptojanin 1 Impairs Clathrin Uncoating at Synapses
Title: Parkinson Sac Domain Mutation in Synaptojanin 1 Impairs Clathrin Uncoating at Synapses and Triggers Dystrophic Changes in Dopaminergic Axons.

report showing expression, location and isoforms of Synj1 and Synj2 in the small and large intestine and that they might participate in intestinal pathology.
Title: The Synaptojanins in the murine small and large intestine.

a novel mechanism by which reduction of a PI(4,5)P2-degrading enzyme, synj1, improves amyloid-induced neuropathology and behavior deficits through accelerating cellular Abeta clearance.
Title: Reduction of synaptojanin 1 accelerates A? clearance and attenuates cognitive deterioration in an Alzheimer mouse model.

These results implicate Pak5 in Pacsin1- and Synaptojanin1-mediated synaptic vesicle trafficking.
Title: Identification of neuronal substrates implicates Pak5 in synaptic vesicle trafficking.

PI(4,5)P-to-PI4P conversion achieved by Synj1 at sites of high curvature may cooperate with dynamin to achieve membrane fission.
Title: Synaptojanin 1-mediated PI(4,5)P2 hydrolysis is modulated by membrane curvature and facilitates membrane fission.

The following Synj1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Synj1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu52623	XM_006522846.3 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu72077	XM_011246085.3 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu72078	XM_011246087.3 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu72079	XM_011246088.3 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu72080	XM_011246089.3 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu79240	XM_017316837.2 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu79241	XM_017316838.2 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu79242	XM_017316839.2 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu79243	XM_017316840.2 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu79244	XM_017316841.2 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu86850	XM_030248907.1 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu86851	XM_030248908.1 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu86852	XM_030248909.1 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu86853	XM_030248910.1 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu12395	NM_001045515.1 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu52615	XM_006522837.4 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu72067	XM_011246075.3 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu72068	XM_011246076.3 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu72069	XM_011246077.3 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu72070	XM_011246078.3 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu72071	XM_011246079.3 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu72072	XM_011246080.3 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu72073	XM_011246081.3 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu72074	XM_011246082.3 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79239	XM_017316836.2 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu86845	XM_030248902.1 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu86846	XM_030248903.1 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu86847	XM_030248904.1 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu86848	XM_030248905.1 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu86849	XM_030248906.1 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu09829	NM_001164483.1 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu72075	XM_011246083.1 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu72076	XM_011246084.1 Latest version!	Mus musculus synaptojanin 1 (Synj1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu52623	XM_006522846.2	Mus musculus synaptojanin 1 (Synj1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu72073	XM_011246081.2	Mus musculus synaptojanin 1 (Synj1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu72080	XM_011246089.2	Mus musculus synaptojanin 1 (Synj1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu72068	XM_011246076.2	Mus musculus synaptojanin 1 (Synj1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu72069	XM_011246077.2	Mus musculus synaptojanin 1 (Synj1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu72077	XM_011246085.2	Mus musculus synaptojanin 1 (Synj1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu79244	XM_017316841.1	Mus musculus synaptojanin 1 (Synj1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu72070	XM_011246078.2	Mus musculus synaptojanin 1 (Synj1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu72071	XM_011246079.2	Mus musculus synaptojanin 1 (Synj1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu72074	XM_011246082.2	Mus musculus synaptojanin 1 (Synj1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu79243	XM_017316840.1	Mus musculus synaptojanin 1 (Synj1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu79239	XM_017316836.1	Mus musculus synaptojanin 1 (Synj1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79240	XM_017316837.1	Mus musculus synaptojanin 1 (Synj1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu72079	XM_011246088.2	Mus musculus synaptojanin 1 (Synj1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu79242	XM_017316839.1	Mus musculus synaptojanin 1 (Synj1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu72067	XM_011246075.2	Mus musculus synaptojanin 1 (Synj1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu52615	XM_006522837.3	Mus musculus synaptojanin 1 (Synj1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu72072	XM_011246080.2	Mus musculus synaptojanin 1 (Synj1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu72078	XM_011246087.2	Mus musculus synaptojanin 1 (Synj1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu79241	XM_017316838.1	Mus musculus synaptojanin 1 (Synj1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Synj1 ( XM_006522846.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522909.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OMu52623
Clone ID Related Accession (Same CDS sequence)	XM_006522846.2 , XM_006522846.3
Accession Version	XM_006522846.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522846.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGAGA ACTTCTACAA ATACTCAAAG CCCAAGAAGA TCCGAGTGTG TGTTGGCACC 
TGGAATGTGA ACGGCGGGAA GCAGTTCCGC AGCATAGCGT TCAAGAACCA GACGCTCACA 
GACTGGCTTC TGGACGCTCC CAAGTTAGCC GGCATCCAGG AGTTTCAAGA TAAGAGAAGT 
AAGCCAACTG ATATATTTGC AATTGGCTTT GAAGAAATGG TGGAGCTGAA TGCTGGGAAC 
ATTGTGAATG CAAGCACAAC CAACCAGAAG CTGTGGGCAG TGGAGCTGCA GAAGACCATC 
TCCAGGGACA ACAAGTATGT CCTGCTGGCC TCGGAGCAGC TGGTGGGTGT CTGCCTGTTT 
GTCTTTATCA GACCACAGCA CGCTCCTTTT ATCAGGGATG TTGCAGTTGA TACTGTGAAA 
ACTGGCATGG GAGGTGCAAC TGGAAACAAG GGCGCCGTTG CAATTCGGAT GCTCTTCCAC 
ACCACCAGCC TTTGCTTCGT CTGCAGCCAC TTTGCTGCAG GGCAGTCCCA AGTCAAAGAG 
AGAAATGAAG ATTTTGTAGA AATAGCACGA AAACTGAGTT TCCCCATGGG ACGAATGCTC 
TTCTCCCATG ACTATGTATT TTGGTGTGGT GATTTCAACT ATCGAATTGA CCTTCCGAAT 
GAAGAGGTTA AAGAGCTCAT AAGACAACAA AACTGGGATT CTCTGATCGC TGGGGATCAG 
CTCATCAACC AGAAAAATGC AGGACAGATC TTTAGAGGAT TCTTAGAGGG AAAAGTAACG 
TTTGCTCCAA CCTATAAATA TGACTTGTTT TCTGAAGACT ATGACACCAG TGAGAAGTGT 
CGCACCCCTG CTTGGACAGA CCGTGTCCTC TGGAGAAGGA GGAAGTGGCC TTTTGACAGA 
TCAGCTGAAG ATTTAGATCT CCTGAATGCT AGTTTCCAAG ATGAAAGTAA AATTCTTTAT 
ACATGGACCC CTGGCACCTT GCTGCACTAT GGAAGAGCCG AGCTGAAGAC TTCTGACCAT 
AGGCCCGTCG TTGCCTTGAT TGACATAGAT ATATTTGAAG TTGAAGCTGA AGAGAGACAA 
AAAATTTATA AAGAAGTTAT TGCCGTCCAG GGCCCACCAG ATGGCACAGT GCTGGTCTCA 
ATCAAAAGTT CTGCACAAGA AAGTACTTTT TTTGATGATG CTTTGATTGA TGAGCTTCTG 
CGGCAGTTTG CACACTTTGG TGAAGTTATA CTCATAAGAT TTGTAGAAGA TAAAATGTGG 
GTTACATTTT TGGAGGGAAG CTCTGCCTTG AATGCTCTGA GCCTAAATGG GAAAGAGCTA 
TTAAATCGGA CTATAACAAT TACTTTAAAA AGTCCAGACT GGATCAAACA TTTGGAAGAA 
GAGATGAGTT TAGAGAAAAT CAGTGTCACG TTGCCCTCAT CAGCAAGCTC CACCCTGCTT 
GGTGAAGACG CAGAGGTCGC AGCAGACTTT GACATGGAAG GTGACGTCGA TGACTACAGC 
GCTGAAGTAG AGGAGCTTCT TCCTCAGCAT CTGCAGCCGT CCTCAAGTTC TGGCCTGGGC 
ACTTCTCCAA GTTCTTCACC CCGGACCAGT CCCTGCCAGT CCCCCACAGT ACCAGAGTAC 
TCCGCCCCTT CTCTTCCCAT CAGACCTAGC CGAGCACCGT CAAGAACTCC AGGACCTCCA 
AGTTCGCAGG GCTCTCCAGT TGACACTCAG CCAGCGGCCC AGAAGGATTC TTCCCAGACT 
CTAGAGCCCA AGAGACCACC CCCTCCCCGC CCAGTCGCTC CTCCGGCACG TCCTGCCCCA 
CCACAGAGAC CACCTCCACC TTCAGGGGCT AGGAGTCCTG CGCCTGCTAG AAAAGAATTT 
GGAGGTGTTG GAGCCCCTCC CAGTCCCGGG GTTGCTAGGA GAGAGATAGA AGCACCCAAA 
AGCCCTGGAA CAGCACGGAA AGATAATATA GGGCGTAATC AGCCTTCCCC TCAAGCCGGA 
CTTGCAGGCC CAGGACCCGC TGGATACGGT GCGGCTAGAC CGACAATTCC AGCTCGTGCT 
GGAGTGATCA GCGCCCCTCA GAGCCAGGCT CGGGTATGTG CTGGAAGGCC GACTCCTGAC 
AGCCAAAGCA AGCCCTCGGA GACATTGAAA GGTCCTGCTG TCCTTCCAGA ACCACTGAAG 
CCTCAGGCTG CATTTCCTCA GCAGCCTTCT CTGCCCACAC CTGCTCAAAA GTTACAGGAC 
CCGCTTGTCC CCATAGCAGC ACCTACTATG CCTCCCTCTG GCCCCCAACC AAATTTGGAA 
ACCCCCCCAC AGCCCCCACC TCGGAGCAGG TCATCTCAAA GCTTGCCTTC AGACTCCTCA 
CCACAGCTGC AGGTAAAAAT AAATGGCATT TCTGGTGTCA AACAAGAGCC AACATTGAAG 
AGTGACCCAT TTGAAGATCT CTCACTTAGT GTGCTTGCTG TATCAAAGGC TCAGCCTTCT 
GTTCAGATTT CACCTGTTCT AACCCCAGAC CCAAAGATGT TGATTCAGTT GCCTTCTGCA 
TCGCAAAGTC AAGTTAACCC TTTGAGTTCT GTAAGTTGCA TGCCAACCAG GCCTCCAGGT 
CCAGAGGAAA GCAAGTCACA GGAGAGTATG GGCAGTTCTG CAAACCCGTT TCCCAGCCTG 
CCCTGCCGGA ACCCTTTTAC AGACAGGACT GCTGCCCCTG GAAACCCATT TAGAGTTCAG 
TCCCAAGAAT CAGAGGCAAC TTCTTGGCTC TCCAAAGAAG AGCCTGTTCC CAACAGTCCC 
TTCCCTCCTC TCATGCCTCT CAGTCATGAC ACGAGCAAGG CTTCAAGTTC GCTTGGTGGC 
TTTGAGGACA ATTTTGATCT GCAGAGCCAG TCTACAGTAA AAACAAGCAA CCCCAAAGGA 
TGGGTAACCT TTGACGAAGA CGACAACTTT CCTACAACAG GAAAGTCAAA GTCAGTTTGT 
CCAGACTTAG TGGGTAACGC GCCAGCTTCA TTTGATGATG ACTGGAGTAA AGGTGCGAGC 
GTTTCTTTCT GTGTGTTGCC AGCCAGAAGG CCACCACCAC CACCACCTCC TGTCCCTCTG 
CTACCACCTG GCACAACCTC ATCTGCAGGA CCTAGCACAA CCCTGCCATC TAAGGCACCT 
TCCACATTGG ACTTCACGGA AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522909.1
CDS	398..3544
Translation	MCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_006522846.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522846.3

ORF Insert Sequence:

ATGTGTGAGA ACTTCTACAA ATACTCAAAG CCCAAGAAGA TCCGAGTGTG TGTTGGCACC 
TGGAATGTGA ACGGCGGGAA GCAGTTCCGC AGCATAGCGT TCAAGAACCA GACGCTCACA 
GACTGGCTTC TGGACGCTCC CAAGTTAGCC GGCATCCAGG AGTTTCAAGA TAAGAGAAGT 
AAGCCAACTG ATATATTTGC AATTGGCTTT GAAGAAATGG TGGAGCTGAA TGCTGGGAAC 
ATTGTGAATG CAAGCACAAC CAACCAGAAG CTGTGGGCAG TGGAGCTGCA GAAGACCATC 
TCCAGGGACA ACAAGTATGT CCTGCTGGCC TCGGAGCAGC TGGTGGGTGT CTGCCTGTTT 
GTCTTTATCA GACCACAGCA CGCTCCTTTT ATCAGGGATG TTGCAGTTGA TACTGTGAAA 
ACTGGCATGG GAGGTGCAAC TGGAAACAAG GGCGCCGTTG CAATTCGGAT GCTCTTCCAC 
ACCACCAGCC TTTGCTTCGT CTGCAGCCAC TTTGCTGCAG GGCAGTCCCA AGTCAAAGAG 
AGAAATGAAG ATTTTGTAGA AATAGCACGA AAACTGAGTT TCCCCATGGG ACGAATGCTC 
TTCTCCCATG ACTATGTATT TTGGTGTGGT GATTTCAACT ATCGAATTGA CCTTCCGAAT 
GAAGAGGTTA AAGAGCTCAT AAGACAACAA AACTGGGATT CTCTGATCGC TGGGGATCAG 
CTCATCAACC AGAAAAATGC AGGACAGATC TTTAGAGGAT TCTTAGAGGG AAAAGTAACG 
TTTGCTCCAA CCTATAAATA TGACTTGTTT TCTGAAGACT ATGACACCAG TGAGAAGTGT 
CGCACCCCTG CTTGGACAGA CCGTGTCCTC TGGAGAAGGA GGAAGTGGCC TTTTGACAGA 
TCAGCTGAAG ATTTAGATCT CCTGAATGCT AGTTTCCAAG ATGAAAGTAA AATTCTTTAT 
ACATGGACCC CTGGCACCTT GCTGCACTAT GGAAGAGCCG AGCTGAAGAC TTCTGACCAT 
AGGCCCGTCG TTGCCTTGAT TGACATAGAT ATATTTGAAG TTGAAGCTGA AGAGAGACAA 
AAAATTTATA AAGAAGTTAT TGCCGTCCAG GGCCCACCAG ATGGCACAGT GCTGGTCTCA 
ATCAAAAGTT CTGCACAAGA AAGTACTTTT TTTGATGATG CTTTGATTGA TGAGCTTCTG 
CGGCAGTTTG CACACTTTGG TGAAGTTATA CTCATAAGAT TTGTAGAAGA TAAAATGTGG 
GTTACATTTT TGGAGGGAAG CTCTGCCTTG AATGCTCTGA GCCTAAATGG GAAAGAGCTA 
TTAAATCGGA CTATAACAAT TACTTTAAAA AGTCCAGACT GGATCAAACA TTTGGAAGAA 
GAGATGAGTT TAGAGAAAAT CAGTGTCACG TTGCCCTCAT CAGCAAGCTC CACCCTGCTT 
GGTGAAGACG CAGAGGTCGC AGCAGACTTT GACATGGAAG GTGACGTCGA TGACTACAGC 
GCTGAAGTAG AGGAGCTTCT TCCTCAGCAT CTGCAGCCGT CCTCAAGTTC TGGCCTGGGC 
ACTTCTCCAA GTTCTTCACC CCGGACCAGT CCCTGCCAGT CCCCCACAGT ACCAGAGTAC 
TCCGCCCCTT CTCTTCCCAT CAGACCTAGC CGAGCACCGT CAAGAACTCC AGGACCTCCA 
AGTTCGCAGG GCTCTCCAGT TGACACTCAG CCAGCGGCCC AGAAGGATTC TTCCCAGACT 
CTAGAGCCCA AGAGACCACC CCCTCCCCGC CCAGTCGCTC CTCCGGCACG TCCTGCCCCA 
CCACAGAGAC CACCTCCACC TTCAGGGGCT AGGAGTCCTG CGCCTGCTAG AAAAGAATTT 
GGAGGTGTTG GAGCCCCTCC CAGTCCCGGG GTTGCTAGGA GAGAGATAGA AGCACCCAAA 
AGCCCTGGAA CAGCACGGAA AGATAATATA GGGCGTAATC AGCCTTCCCC TCAAGCCGGA 
CTTGCAGGCC CAGGACCCGC TGGATACGGT GCGGCTAGAC CGACAATTCC AGCTCGTGCT 
GGAGTGATCA GCGCCCCTCA GAGCCAGGCT CGGGTATGTG CTGGAAGGCC GACTCCTGAC 
AGCCAAAGCA AGCCCTCGGA GACATTGAAA GGTCCTGCTG TCCTTCCAGA ACCACTGAAG 
CCTCAGGCTG CATTTCCTCA GCAGCCTTCT CTGCCCACAC CTGCTCAAAA GTTACAGGAC 
CCGCTTGTCC CCATAGCAGC ACCTACTATG CCTCCCTCTG GCCCCCAACC AAATTTGGAA 
ACCCCCCCAC AGCCCCCACC TCGGAGCAGG TCATCTCAAA GCTTGCCTTC AGACTCCTCA 
CCACAGCTGC AGGTAAAAAT AAATGGCATT TCTGGTGTCA AACAAGAGCC AACATTGAAG 
AGTGACCCAT TTGAAGATCT CTCACTTAGT GTGCTTGCTG TATCAAAGGC TCAGCCTTCT 
GTTCAGATTT CACCTGTTCT AACCCCAGAC CCAAAGATGT TGATTCAGTT GCCTTCTGCA 
TCGCAAAGTC AAGTTAACCC TTTGAGTTCT GTAAGTTGCA TGCCAACCAG GCCTCCAGGT 
CCAGAGGAAA GCAAGTCACA GGAGAGTATG GGCAGTTCTG CAAACCCGTT TCCCAGCCTG 
CCCTGCCGGA ACCCTTTTAC AGACAGGACT GCTGCCCCTG GAAACCCATT TAGAGTTCAG 
TCCCAAGAAT CAGAGGCAAC TTCTTGGCTC TCCAAAGAAG AGCCTGTTCC CAACAGTCCC 
TTCCCTCCTC TCATGCCTCT CAGTCATGAC ACGAGCAAGG CTTCAAGTTC GCTTGGTGGC 
TTTGAGGACA ATTTTGATCT GCAGAGCCAG TCTACAGTAA AAACAAGCAA CCCCAAAGGA 
TGGGTAACCT TTGACGAAGA CGACAACTTT CCTACAACAG GAAAGTCAAA GTCAGTTTGT 
CCAGACTTAG TGGGTAACGC GCCAGCTTCA TTTGATGATG ACTGGAGTAA AGGTGCGAGC 
GTTTCTTTCT GTGTGTTGCC AGCCAGAAGG CCACCACCAC CACCACCTCC TGTCCCTCTG 
CTACCACCTG GCACAACCTC ATCTGCAGGA CCTAGCACAA CCCTGCCATC TAAGGCACCT 
TCCACATTGG ACTTCACGGA AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246085.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244387.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X19, mRNA.

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CloneID	OMu72077
Clone ID Related Accession (Same CDS sequence)	XM_011246085.2 , XM_011246085.3
Accession Version	XM_011246085.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246085.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG GAGCAACCAA CAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244387.1
CDS	2226..6593
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQEQPTG

Target ORF information:

RefSeq Version	XM_011246085.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246085.3

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG GAGCAACCAA CAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246087.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244389.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OMu72078
Clone ID Related Accession (Same CDS sequence)	XM_011246087.2 , XM_011246087.3
Accession Version	XM_011246087.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246087.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG AATCAGTGCT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244389.1
CDS	2226..6590
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQNQCS

Target ORF information:

RefSeq Version	XM_011246087.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246087.3

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG AATCAGTGCT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246088.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244390.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OMu72079
Clone ID Related Accession (Same CDS sequence)	XM_011246088.3 , XM_011246088.2
Accession Version	XM_011246088.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246088.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGGTGTTGG AGCCCCTCCC AGTCCCGGGG TTGCTAGGAG AGAGATAGAA 
GCACCCAAAA GCCCTGGAAC AGCACGGAAA GATAATATAG GGCGTAATCA GCCTTCCCCT 
CAAGCCGGAC TTGCAGGCCC AGGACCCGCT GGATACGGTG CGGCTAGACC GACAATTCCA 
GCTCGTGCTG GAGTGATCAG CGCCCCTCAG AGCCAGGCTC GGGTATGTGC TGGAAGGCCG 
ACTCCTGACA GCCAAAGCAA GCCCTCGGAG ACATTGAAAG GTCCTGCTGT CCTTCCAGAA 
CCACTGAAGC CTCAGGCTGC ATTTCCTCAG CAGCCTTCTC TGCCCACACC TGCTCAAAAG 
TTACAGGACC CGCTTGTCCC CATAGCAGCA CCTACTATGC CTCCCTCTGG CCCCCAACCA 
AATTTGGAAA CCCCCCCACA GCCCCCACCT CGGAGCAGGT CATCTCAAAG CTTGCCTTCA 
GACTCCTCAC CACAGCTGCA GCAGGAGCAA CCAACAGGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244390.1
CDS	2226..6587
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	XM_011246088.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246088.3

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGGTGTTGG AGCCCCTCCC AGTCCCGGGG TTGCTAGGAG AGAGATAGAA 
GCACCCAAAA GCCCTGGAAC AGCACGGAAA GATAATATAG GGCGTAATCA GCCTTCCCCT 
CAAGCCGGAC TTGCAGGCCC AGGACCCGCT GGATACGGTG CGGCTAGACC GACAATTCCA 
GCTCGTGCTG GAGTGATCAG CGCCCCTCAG AGCCAGGCTC GGGTATGTGC TGGAAGGCCG 
ACTCCTGACA GCCAAAGCAA GCCCTCGGAG ACATTGAAAG GTCCTGCTGT CCTTCCAGAA 
CCACTGAAGC CTCAGGCTGC ATTTCCTCAG CAGCCTTCTC TGCCCACACC TGCTCAAAAG 
TTACAGGACC CGCTTGTCCC CATAGCAGCA CCTACTATGC CTCCCTCTGG CCCCCAACCA 
AATTTGGAAA CCCCCCCACA GCCCCCACCT CGGAGCAGGT CATCTCAAAG CTTGCCTTCA 
GACTCCTCAC CACAGCTGCA GCAGGAGCAA CCAACAGGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246089.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244391.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X31, mRNA.

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CloneID	OMu72080
Clone ID Related Accession (Same CDS sequence)	XM_011246089.3 , XM_011246089.2
Accession Version	XM_011246089.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246089.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA AGTTAAAAGA TGGTGCGCGA TCTGTCACCA GAACCATCCA GAATAACTTC 
TTCGACAGCT CCAAGCAGGA AGCCATTGAT GTCCTGCTCC TGGGAAATAC TCTCAACAGC 
GATTTAGCTG ACAAAGCCCG AGCGCTTCTA ACTACTGGAA GTTTGCGTGT TTCTGAACAG 
ACATTACAGT CAGCATCTTC CAAAGTCTTA AAGAACATGT GTGAGAACTT CTACAAATAC 
TCAAAGCCCA AGAAGATCCG AGTGTGTGTT GGCACCTGGA ATGTGAACGG CGGGAAGCAG 
TTCCGCAGCA TAGCGTTCAA GAACCAGACG CTCACAGACT GGCTTCTGGA CGCTCCCAAG 
TTAGCCGGCA TCCAGGAGTT TCAAGATAAG AGAAGTAAGC CAACTGATAT ATTTGCAATT 
GGCTTTGAAG AAATGGTGGA GCTGAATGCT GGGAACATTG TGAATGCAAG CACAACCAAC 
CAGAAGCTGT GGGCAGTGGA GCTGCAGAAG ACCATCTCCA GGGACAACAA GTATGTCCTG 
CTGGCCTCGG AGCAGCTGGT GGGTGTCTGC CTGTTTGTCT TTATCAGACC ACAGCACGCT 
CCTTTTATCA GGGATGTTGC AGTTGATACT GTGAAAACTG GCATGGGAGG TGCAACTGGA 
AACAAGGGCG CCGTTGCAAT TCGGATGCTC TTCCACACCA CCAGCCTTTG CTTCGTCTGC 
AGCCACTTTG CTGCAGGGCA GTCCCAAGTC AAAGAGAGAA ATGAAGATTT TGTAGAAATA 
GCACGAAAAC TGAGTTTCCC CATGGGACGA ATGCTCTTCT CCCATGACTA TGTATTTTGG 
TGTGGTGATT TCAACTATCG AATTGACCTT CCGAATGAAG AGGTTAAAGA GCTCATAAGA 
CAACAAAACT GGGATTCTCT GATCGCTGGG GATCAGCTCA TCAACCAGAA AAATGCAGGA 
CAGATCTTTA GAGGATTCTT AGAGGGAAAA GTAACGTTTG CTCCAACCTA TAAATATGAC 
TTGTTTTCTG AAGACTATGA CACCAGTGAG AAGTGTCGCA CCCCTGCTTG GACAGACCGT 
GTCCTCTGGA GAAGGAGGAA GTGGCCTTTT GACAGATCAG CTGAAGATTT AGATCTCCTG 
AATGCTAGTT TCCAAGATGA AAGTAAAATT CTTTATACAT GGACCCCTGG CACCTTGCTG 
CACTATGGAA GAGCCGAGCT GAAGACTTCT GACCATAGGC CCGTCGTTGC CTTGATTGAC 
ATAGATATAT TTGAAGTTGA AGCTGAAGAG AGACAAAAAA TTTATAAAGA AGTTATTGCC 
GTCCAGGGCC CACCAGATGG CACAGTGCTG GTCTCAATCA AAAGTTCTGC ACAAGAAAGT 
ACTTTTTTTG ATGATGCTTT GATTGATGAG CTTCTGCGGC AGTTTGCACA CTTTGGTGAA 
GTTATACTCA TAAGATTTGT AGAAGATAAA ATGTGGGTTA CATTTTTGGA GGGAAGCTCT 
GCCTTGAATG CTCTGAGCCT AAATGGGAAA GAGCTATTAA ATCGGACTAT AACAATTACT 
TTAAAAAGTC CAGACTGGAT CAAACATTTG GAAGAAGAGA TGAGTTTAGA GAAAATCAGT 
GTCACGTTGC CCTCATCAGC AAGCTCCACC CTGCTTGGTG AAGACGCAGA GGTCGCAGCA 
GACTTTGACA TGGAAGGTGA CGTCGATGAC TACAGCGCTG AAGTAGAGGA GCTTCTTCCT 
CAGCATCTGC AGCCGTCCTC AAGTTCTGGC CTGGGCACTT CTCCAAGTTC TTCACCCCGG 
ACCAGTCCCT GCCAGTCCCC CACAGTACCA GAGTACTCCG CCCCTTCTCT TCCCATCAGA 
CCTAGCCGAG CACCGTCAAG AACTCCAGGA CCTCCAAGTT CGCAGGGCTC TCCAGTTGAC 
ACTCAGCCAG CGGCCCAGAA GGATTCTTCC CAGACTCTAG AGCCCAAGAG ACCACCCCCT 
CCCCGCCCAG TCGCTCCTCC GGCACGTCCT GCCCCACCAC AGAGACCACC TCCACCTTCA 
GGGGCTAGGA GTCCTGCGCC TGCTAGAAAA GAATTTGGAG GTGTTGGAGC CCCTCCCAGT 
CCCGGGGTTG CTAGGAGAGA GATAGAAGCA CCCAAAAGCC CTGGAACAGC ACGGAAAGAT 
AATATAGGGC GTAATCAGCC TTCCCCTCAA GCCGGACTTG CAGGCCCAGG ACCCGCTGGA 
TACGGTGCGG CTAGACCGAC AATTCCAGCT CGTGCTGGAG TGATCAGCGC CCCTCAGAGC 
CAGGCTCGGG TATGTGCTGG AAGGCCGACT CCTGACAGCC AAAGCAAGCC CTCGGAGACA 
TTGAAAGGTC CTGCTGTCCT TCCAGAACCA CTGAAGCCTC AGGCTGCATT TCCTCAGCAG 
CCTTCTCTGC CCACACCTGC TCAAAAGTTA CAGGACCCGC TTGTCCCCAT AGCAGCACCT 
ACTATGCCTC CCTCTGGCCC CCAACCAAAT TTGGAAACCC CCCCACAGCC CCCACCTCGG 
AGCAGGTCAT CTCAAAGCTT GCCTTCAGAC TCCTCACCAC AGCTGCAGGT AAAAATAAAT 
GGCATTTCTG GTGTCAAACA AGAGCCAACA TTGAAGAGTG ACCCATTTGA AGATCTCTCA 
CTTAGTGTGC TTGCTGTATC AAAGGCTCAG CCTTCTGTTC AGATTTCACC TGTTCTAACC 
CCAGACCCAA AGATGTTGAT TCAGTTGCCT TCTGCATCGC AAAGTCAAGT TAACCCTTTG 
AGTTCTGTAA GTTGCATGCC AACCAGGCCT CCAGGTCCAG AGGAAAGCAA GTCACAGGAG 
AGTATGGGCA GTTCTGCAAA CCCGTTTCCC AGCCTGCCCT GCCGGAACCC TTTTACAGAC 
AGGACTGCTG CCCCTGGAAA CCCATTTAGA GTTCAGTCCC AAGAATCAGA GGCAACTTCT 
TGGCTCTCCA AAGAAGAGCC TGTTCCCAAC AGTCCCTTCC CTCCTCTCAT GCCTCTCAGT 
CATGACACGA GCAAGGCTTC AAGTTCGCTT GGTGGCTTTG AGGACAATTT TGATCTGCAG 
AGCCAGTCTA CAGTAAAAAC AAGCAACCCC AAAGGATGGG TAACCTTTGA CGAAGACGAC 
AACTTTCCTA CAACAGGAAA GTCAAAGTCA GTTTGTCCAG ACTTAGTGGG TAACGCGCCA 
GCTTCATTTG ATGATGACTG GAGTAAAGGT GCGAGCGTTT CTTTCTGTGT GTTGCCAGCC 
AGAAGGCCAC CACCACCACC ACCTCCTGTC CCTCTGCTAC CACCTGGCAC AACCTCATCT 
GCAGGACCTA GCACAACCCT GCCATCTAAG GCACCTTCCA CATTGGACTT CACGGAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244391.1
CDS	1666..5028
Translation	MAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246089.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246089.3

ORF Insert Sequence:

ATGGCTGGAA AGTTAAAAGA TGGTGCGCGA TCTGTCACCA GAACCATCCA GAATAACTTC 
TTCGACAGCT CCAAGCAGGA AGCCATTGAT GTCCTGCTCC TGGGAAATAC TCTCAACAGC 
GATTTAGCTG ACAAAGCCCG AGCGCTTCTA ACTACTGGAA GTTTGCGTGT TTCTGAACAG 
ACATTACAGT CAGCATCTTC CAAAGTCTTA AAGAACATGT GTGAGAACTT CTACAAATAC 
TCAAAGCCCA AGAAGATCCG AGTGTGTGTT GGCACCTGGA ATGTGAACGG CGGGAAGCAG 
TTCCGCAGCA TAGCGTTCAA GAACCAGACG CTCACAGACT GGCTTCTGGA CGCTCCCAAG 
TTAGCCGGCA TCCAGGAGTT TCAAGATAAG AGAAGTAAGC CAACTGATAT ATTTGCAATT 
GGCTTTGAAG AAATGGTGGA GCTGAATGCT GGGAACATTG TGAATGCAAG CACAACCAAC 
CAGAAGCTGT GGGCAGTGGA GCTGCAGAAG ACCATCTCCA GGGACAACAA GTATGTCCTG 
CTGGCCTCGG AGCAGCTGGT GGGTGTCTGC CTGTTTGTCT TTATCAGACC ACAGCACGCT 
CCTTTTATCA GGGATGTTGC AGTTGATACT GTGAAAACTG GCATGGGAGG TGCAACTGGA 
AACAAGGGCG CCGTTGCAAT TCGGATGCTC TTCCACACCA CCAGCCTTTG CTTCGTCTGC 
AGCCACTTTG CTGCAGGGCA GTCCCAAGTC AAAGAGAGAA ATGAAGATTT TGTAGAAATA 
GCACGAAAAC TGAGTTTCCC CATGGGACGA ATGCTCTTCT CCCATGACTA TGTATTTTGG 
TGTGGTGATT TCAACTATCG AATTGACCTT CCGAATGAAG AGGTTAAAGA GCTCATAAGA 
CAACAAAACT GGGATTCTCT GATCGCTGGG GATCAGCTCA TCAACCAGAA AAATGCAGGA 
CAGATCTTTA GAGGATTCTT AGAGGGAAAA GTAACGTTTG CTCCAACCTA TAAATATGAC 
TTGTTTTCTG AAGACTATGA CACCAGTGAG AAGTGTCGCA CCCCTGCTTG GACAGACCGT 
GTCCTCTGGA GAAGGAGGAA GTGGCCTTTT GACAGATCAG CTGAAGATTT AGATCTCCTG 
AATGCTAGTT TCCAAGATGA AAGTAAAATT CTTTATACAT GGACCCCTGG CACCTTGCTG 
CACTATGGAA GAGCCGAGCT GAAGACTTCT GACCATAGGC CCGTCGTTGC CTTGATTGAC 
ATAGATATAT TTGAAGTTGA AGCTGAAGAG AGACAAAAAA TTTATAAAGA AGTTATTGCC 
GTCCAGGGCC CACCAGATGG CACAGTGCTG GTCTCAATCA AAAGTTCTGC ACAAGAAAGT 
ACTTTTTTTG ATGATGCTTT GATTGATGAG CTTCTGCGGC AGTTTGCACA CTTTGGTGAA 
GTTATACTCA TAAGATTTGT AGAAGATAAA ATGTGGGTTA CATTTTTGGA GGGAAGCTCT 
GCCTTGAATG CTCTGAGCCT AAATGGGAAA GAGCTATTAA ATCGGACTAT AACAATTACT 
TTAAAAAGTC CAGACTGGAT CAAACATTTG GAAGAAGAGA TGAGTTTAGA GAAAATCAGT 
GTCACGTTGC CCTCATCAGC AAGCTCCACC CTGCTTGGTG AAGACGCAGA GGTCGCAGCA 
GACTTTGACA TGGAAGGTGA CGTCGATGAC TACAGCGCTG AAGTAGAGGA GCTTCTTCCT 
CAGCATCTGC AGCCGTCCTC AAGTTCTGGC CTGGGCACTT CTCCAAGTTC TTCACCCCGG 
ACCAGTCCCT GCCAGTCCCC CACAGTACCA GAGTACTCCG CCCCTTCTCT TCCCATCAGA 
CCTAGCCGAG CACCGTCAAG AACTCCAGGA CCTCCAAGTT CGCAGGGCTC TCCAGTTGAC 
ACTCAGCCAG CGGCCCAGAA GGATTCTTCC CAGACTCTAG AGCCCAAGAG ACCACCCCCT 
CCCCGCCCAG TCGCTCCTCC GGCACGTCCT GCCCCACCAC AGAGACCACC TCCACCTTCA 
GGGGCTAGGA GTCCTGCGCC TGCTAGAAAA GAATTTGGAG GTGTTGGAGC CCCTCCCAGT 
CCCGGGGTTG CTAGGAGAGA GATAGAAGCA CCCAAAAGCC CTGGAACAGC ACGGAAAGAT 
AATATAGGGC GTAATCAGCC TTCCCCTCAA GCCGGACTTG CAGGCCCAGG ACCCGCTGGA 
TACGGTGCGG CTAGACCGAC AATTCCAGCT CGTGCTGGAG TGATCAGCGC CCCTCAGAGC 
CAGGCTCGGG TATGTGCTGG AAGGCCGACT CCTGACAGCC AAAGCAAGCC CTCGGAGACA 
TTGAAAGGTC CTGCTGTCCT TCCAGAACCA CTGAAGCCTC AGGCTGCATT TCCTCAGCAG 
CCTTCTCTGC CCACACCTGC TCAAAAGTTA CAGGACCCGC TTGTCCCCAT AGCAGCACCT 
ACTATGCCTC CCTCTGGCCC CCAACCAAAT TTGGAAACCC CCCCACAGCC CCCACCTCGG 
AGCAGGTCAT CTCAAAGCTT GCCTTCAGAC TCCTCACCAC AGCTGCAGGT AAAAATAAAT 
GGCATTTCTG GTGTCAAACA AGAGCCAACA TTGAAGAGTG ACCCATTTGA AGATCTCTCA 
CTTAGTGTGC TTGCTGTATC AAAGGCTCAG CCTTCTGTTC AGATTTCACC TGTTCTAACC 
CCAGACCCAA AGATGTTGAT TCAGTTGCCT TCTGCATCGC AAAGTCAAGT TAACCCTTTG 
AGTTCTGTAA GTTGCATGCC AACCAGGCCT CCAGGTCCAG AGGAAAGCAA GTCACAGGAG 
AGTATGGGCA GTTCTGCAAA CCCGTTTCCC AGCCTGCCCT GCCGGAACCC TTTTACAGAC 
AGGACTGCTG CCCCTGGAAA CCCATTTAGA GTTCAGTCCC AAGAATCAGA GGCAACTTCT 
TGGCTCTCCA AAGAAGAGCC TGTTCCCAAC AGTCCCTTCC CTCCTCTCAT GCCTCTCAGT 
CATGACACGA GCAAGGCTTC AAGTTCGCTT GGTGGCTTTG AGGACAATTT TGATCTGCAG 
AGCCAGTCTA CAGTAAAAAC AAGCAACCCC AAAGGATGGG TAACCTTTGA CGAAGACGAC 
AACTTTCCTA CAACAGGAAA GTCAAAGTCA GTTTGTCCAG ACTTAGTGGG TAACGCGCCA 
GCTTCATTTG ATGATGACTG GAGTAAAGGT GCGAGCGTTT CTTTCTGTGT GTTGCCAGCC 
AGAAGGCCAC CACCACCACC ACCTCCTGTC CCTCTGCTAC CACCTGGCAC AACCTCATCT 
GCAGGACCTA GCACAACCCT GCCATCTAAG GCACCTTCCA CATTGGACTT CACGGAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_017316837.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172326.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OMu79240
Clone ID Related Accession (Same CDS sequence)	XM_017316837.2 , XM_017316837.1
Accession Version	XM_017316837.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316837.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG CAGGAGCAAC CAACAGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172326.1
CDS	2226..6596
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	XM_017316837.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316837.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG CAGGAGCAAC CAACAGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_017316837.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172326.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu79240
Clone ID Related Accession (Same CDS sequence)	XM_017316837.2 , XM_017316837.1
Accession Version	XM_017316837.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG CAGGAGCAAC CAACAGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172326.1
CDS	632..5002
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	XM_017316837.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316837.1

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG CAGGAGCAAC CAACAGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_017316838.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172327.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X18, mRNA.

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CloneID	OMu79241
Clone ID Related Accession (Same CDS sequence)	XM_017316838.1 , XM_017316838.2
Accession Version	XM_017316838.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGCACCCAA AAGCCCTGGA ACAGCACGGA AAGATAATAT AGGGCGTAAT 
CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC CCAGGACCCG CTGGATACGG TGCGGCTAGA 
CCGACAATTC CAGCTCGTGC TGGAGTGATC AGCGCCCCTC AGAGCCAGGC TCGGGTATGT 
GCTGGAAGGC CGACTCCTGA CAGCCAAAGC AAGCCCTCGG AGACATTGAA AGGTCCTGCT 
GTCCTTCCAG AACCACTGAA GCCTCAGGCT GCATTTCCTC AGCAGCCTTC TCTGCCCACA 
CCTGCTCAAA AGTTACAGGA CCCGCTTGTC CCCATAGCAG CACCTACTAT GCCTCCCTCT 
GGCCCCCAAC CAAATTTGGA AACCCCCCCA CAGCCCCCAC CTCGGAGCAG GTCATCTCAA 
AGCTTGCCTT CAGACTCCTC ACCACAGCTG CAGCAGGAGC AACCAACAGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172327.1
CDS	631..4944
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	XM_017316838.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316838.1

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGCACCCAA AAGCCCTGGA ACAGCACGGA AAGATAATAT AGGGCGTAAT 
CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC CCAGGACCCG CTGGATACGG TGCGGCTAGA 
CCGACAATTC CAGCTCGTGC TGGAGTGATC AGCGCCCCTC AGAGCCAGGC TCGGGTATGT 
GCTGGAAGGC CGACTCCTGA CAGCCAAAGC AAGCCCTCGG AGACATTGAA AGGTCCTGCT 
GTCCTTCCAG AACCACTGAA GCCTCAGGCT GCATTTCCTC AGCAGCCTTC TCTGCCCACA 
CCTGCTCAAA AGTTACAGGA CCCGCTTGTC CCCATAGCAG CACCTACTAT GCCTCCCTCT 
GGCCCCCAAC CAAATTTGGA AACCCCCCCA CAGCCCCCAC CTCGGAGCAG GTCATCTCAA 
AGCTTGCCTT CAGACTCCTC ACCACAGCTG CAGCAGGAGC AACCAACAGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_017316838.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172327.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X24, mRNA.

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CloneID	OMu79241
Clone ID Related Accession (Same CDS sequence)	XM_017316838.1 , XM_017316838.2
Accession Version	XM_017316838.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316838.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGCACCCAA AAGCCCTGGA ACAGCACGGA AAGATAATAT AGGGCGTAAT 
CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC CCAGGACCCG CTGGATACGG TGCGGCTAGA 
CCGACAATTC CAGCTCGTGC TGGAGTGATC AGCGCCCCTC AGAGCCAGGC TCGGGTATGT 
GCTGGAAGGC CGACTCCTGA CAGCCAAAGC AAGCCCTCGG AGACATTGAA AGGTCCTGCT 
GTCCTTCCAG AACCACTGAA GCCTCAGGCT GCATTTCCTC AGCAGCCTTC TCTGCCCACA 
CCTGCTCAAA AGTTACAGGA CCCGCTTGTC CCCATAGCAG CACCTACTAT GCCTCCCTCT 
GGCCCCCAAC CAAATTTGGA AACCCCCCCA CAGCCCCCAC CTCGGAGCAG GTCATCTCAA 
AGCTTGCCTT CAGACTCCTC ACCACAGCTG CAGCAGGAGC AACCAACAGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172327.1
CDS	2226..6539
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	XM_017316838.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316838.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGCACCCAA AAGCCCTGGA ACAGCACGGA AAGATAATAT AGGGCGTAAT 
CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC CCAGGACCCG CTGGATACGG TGCGGCTAGA 
CCGACAATTC CAGCTCGTGC TGGAGTGATC AGCGCCCCTC AGAGCCAGGC TCGGGTATGT 
GCTGGAAGGC CGACTCCTGA CAGCCAAAGC AAGCCCTCGG AGACATTGAA AGGTCCTGCT 
GTCCTTCCAG AACCACTGAA GCCTCAGGCT GCATTTCCTC AGCAGCCTTC TCTGCCCACA 
CCTGCTCAAA AGTTACAGGA CCCGCTTGTC CCCATAGCAG CACCTACTAT GCCTCCCTCT 
GGCCCCCAAC CAAATTTGGA AACCCCCCCA CAGCCCCCAC CTCGGAGCAG GTCATCTCAA 
AGCTTGCCTT CAGACTCCTC ACCACAGCTG CAGCAGGAGC AACCAACAGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_017316839.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172328.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OMu79242
Clone ID Related Accession (Same CDS sequence)	XM_017316839.2 , XM_017316839.1
Accession Version	XM_017316839.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316839.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGCGTAA TCAGCCTTCC 
CCTCAAGCCG GACTTGCAGG CCCAGGACCC GCTGGATACG GTGCGGCTAG ACCGACAATT 
CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT CAGAGCCAGG CTCGGGTATG TGCTGGAAGG 
CCGACTCCTG ACAGCCAAAG CAAGCCCTCG GAGACATTGA AAGGTCCTGC TGTCCTTCCA 
GAACCACTGA AGCCTCAGGC TGCATTTCCT CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA 
AAGTTACAGG ACCCGCTTGT CCCCATAGCA GCACCTACTA TGCCTCCCTC TGGCCCCCAA 
CCAAATTTGG AAACCCCCCC ACAGCCCCCA CCTCGGAGCA GGTCATCTCA AAGCTTGCCT 
TCAGACTCCT CACCACAGCT GCAGCAGGAG CAACCAACAG GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172328.1
CDS	2226..6470
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	XM_017316839.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316839.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGCGTAA TCAGCCTTCC 
CCTCAAGCCG GACTTGCAGG CCCAGGACCC GCTGGATACG GTGCGGCTAG ACCGACAATT 
CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT CAGAGCCAGG CTCGGGTATG TGCTGGAAGG 
CCGACTCCTG ACAGCCAAAG CAAGCCCTCG GAGACATTGA AAGGTCCTGC TGTCCTTCCA 
GAACCACTGA AGCCTCAGGC TGCATTTCCT CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA 
AAGTTACAGG ACCCGCTTGT CCCCATAGCA GCACCTACTA TGCCTCCCTC TGGCCCCCAA 
CCAAATTTGG AAACCCCCCC ACAGCCCCCA CCTCGGAGCA GGTCATCTCA AAGCTTGCCT 
TCAGACTCCT CACCACAGCT GCAGCAGGAG CAACCAACAG GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_017316839.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172328.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OMu79242
Clone ID Related Accession (Same CDS sequence)	XM_017316839.2 , XM_017316839.1
Accession Version	XM_017316839.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGCGTAA TCAGCCTTCC 
CCTCAAGCCG GACTTGCAGG CCCAGGACCC GCTGGATACG GTGCGGCTAG ACCGACAATT 
CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT CAGAGCCAGG CTCGGGTATG TGCTGGAAGG 
CCGACTCCTG ACAGCCAAAG CAAGCCCTCG GAGACATTGA AAGGTCCTGC TGTCCTTCCA 
GAACCACTGA AGCCTCAGGC TGCATTTCCT CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA 
AAGTTACAGG ACCCGCTTGT CCCCATAGCA GCACCTACTA TGCCTCCCTC TGGCCCCCAA 
CCAAATTTGG AAACCCCCCC ACAGCCCCCA CCTCGGAGCA GGTCATCTCA AAGCTTGCCT 
TCAGACTCCT CACCACAGCT GCAGCAGGAG CAACCAACAG GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172328.1
CDS	630..4874
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	XM_017316839.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316839.1

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGCGTAA TCAGCCTTCC 
CCTCAAGCCG GACTTGCAGG CCCAGGACCC GCTGGATACG GTGCGGCTAG ACCGACAATT 
CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT CAGAGCCAGG CTCGGGTATG TGCTGGAAGG 
CCGACTCCTG ACAGCCAAAG CAAGCCCTCG GAGACATTGA AAGGTCCTGC TGTCCTTCCA 
GAACCACTGA AGCCTCAGGC TGCATTTCCT CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA 
AAGTTACAGG ACCCGCTTGT CCCCATAGCA GCACCTACTA TGCCTCCCTC TGGCCCCCAA 
CCAAATTTGG AAACCCCCCC ACAGCCCCCA CCTCGGAGCA GGTCATCTCA AAGCTTGCCT 
TCAGACTCCT CACCACAGCT GCAGCAGGAG CAACCAACAG GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_017316840.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172329.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X20, mRNA.

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CloneID	OMu79243
Clone ID Related Accession (Same CDS sequence)	XM_017316840.1 , XM_017316840.2
Accession Version	XM_017316840.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGCGTA ATCAGCCTTC CCCTCAAGCC 
GGACTTGCAG GCCCAGGACC CGCTGGATAC GGTGCGGCTA GACCGACAAT TCCAGCTCGT 
GCTGGAGTGA TCAGCGCCCC TCAGAGCCAG GCTCGGGTAT GTGCTGGAAG GCCGACTCCT 
GACAGCCAAA GCAAGCCCTC GGAGACATTG AAAGGTCCTG CTGTCCTTCC AGAACCACTG 
AAGCCTCAGG CTGCATTTCC TCAGCAGCCT TCTCTGCCCA CACCTGCTCA AAAGTTACAG 
GACCCGCTTG TCCCCATAGC AGCACCTACT ATGCCTCCCT CTGGCCCCCA ACCAAATTTG 
GAAACCCCCC CACAGCCCCC ACCTCGGAGC AGGTCATCTC AAAGCTTGCC TTCAGACTCC 
TCACCACAGC TGCAGCAGGA GCAACCAACA GGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172329.1
CDS	629..4864
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	XM_017316840.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316840.1

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGCGTA ATCAGCCTTC CCCTCAAGCC 
GGACTTGCAG GCCCAGGACC CGCTGGATAC GGTGCGGCTA GACCGACAAT TCCAGCTCGT 
GCTGGAGTGA TCAGCGCCCC TCAGAGCCAG GCTCGGGTAT GTGCTGGAAG GCCGACTCCT 
GACAGCCAAA GCAAGCCCTC GGAGACATTG AAAGGTCCTG CTGTCCTTCC AGAACCACTG 
AAGCCTCAGG CTGCATTTCC TCAGCAGCCT TCTCTGCCCA CACCTGCTCA AAAGTTACAG 
GACCCGCTTG TCCCCATAGC AGCACCTACT ATGCCTCCCT CTGGCCCCCA ACCAAATTTG 
GAAACCCCCC CACAGCCCCC ACCTCGGAGC AGGTCATCTC AAAGCTTGCC TTCAGACTCC 
TCACCACAGC TGCAGCAGGA GCAACCAACA GGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_017316840.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172329.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X29, mRNA.

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CloneID	OMu79243
Clone ID Related Accession (Same CDS sequence)	XM_017316840.1 , XM_017316840.2
Accession Version	XM_017316840.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316840.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGCGTA ATCAGCCTTC CCCTCAAGCC 
GGACTTGCAG GCCCAGGACC CGCTGGATAC GGTGCGGCTA GACCGACAAT TCCAGCTCGT 
GCTGGAGTGA TCAGCGCCCC TCAGAGCCAG GCTCGGGTAT GTGCTGGAAG GCCGACTCCT 
GACAGCCAAA GCAAGCCCTC GGAGACATTG AAAGGTCCTG CTGTCCTTCC AGAACCACTG 
AAGCCTCAGG CTGCATTTCC TCAGCAGCCT TCTCTGCCCA CACCTGCTCA AAAGTTACAG 
GACCCGCTTG TCCCCATAGC AGCACCTACT ATGCCTCCCT CTGGCCCCCA ACCAAATTTG 
GAAACCCCCC CACAGCCCCC ACCTCGGAGC AGGTCATCTC AAAGCTTGCC TTCAGACTCC 
TCACCACAGC TGCAGCAGGA GCAACCAACA GGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172329.1
CDS	2226..6461
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	XM_017316840.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316840.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGCGTA ATCAGCCTTC CCCTCAAGCC 
GGACTTGCAG GCCCAGGACC CGCTGGATAC GGTGCGGCTA GACCGACAAT TCCAGCTCGT 
GCTGGAGTGA TCAGCGCCCC TCAGAGCCAG GCTCGGGTAT GTGCTGGAAG GCCGACTCCT 
GACAGCCAAA GCAAGCCCTC GGAGACATTG AAAGGTCCTG CTGTCCTTCC AGAACCACTG 
AAGCCTCAGG CTGCATTTCC TCAGCAGCCT TCTCTGCCCA CACCTGCTCA AAAGTTACAG 
GACCCGCTTG TCCCCATAGC AGCACCTACT ATGCCTCCCT CTGGCCCCCA ACCAAATTTG 
GAAACCCCCC CACAGCCCCC ACCTCGGAGC AGGTCATCTC AAAGCTTGCC TTCAGACTCC 
TCACCACAGC TGCAGCAGGA GCAACCAACA GGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_017316841.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172330.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OMu79244
Clone ID Related Accession (Same CDS sequence)	XM_017316841.1 , XM_017316841.2
Accession Version	XM_017316841.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GCATCTTCCA AAGTCTTAAA GAACATGTGT 
GAGAACTTCT ACAAATACTC AAAGCCCAAG AAGATCCGAG TGTGTGTTGG CACCTGGAAT 
GTGAACGGCG GGAAGCAGTT CCGCAGCATA GCGTTCAAGA ACCAGACGCT CACAGACTGG 
CTTCTGGACG CTCCCAAGTT AGCCGGCATC CAGGAGTTTC AAGATAAGAG AAGTAAGCCA 
ACTGATATAT TTGCAATTGG CTTTGAAGAA ATGGTGGAGC TGAATGCTGG GAACATTGTG 
AATGCAAGCA CAACCAACCA GAAGCTGTGG GCAGTGGAGC TGCAGAAGAC CATCTCCAGG 
GACAACAAGT ATGTCCTGCT GGCCTCGGAG CAGCTGGTGG GTGTCTGCCT GTTTGTCTTT 
ATCAGACCAC AGCACGCTCC TTTTATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGC 
ATGGGAGGTG CAACTGGAAA CAAGGGCGCC GTTGCAATTC GGATGCTCTT CCACACCACC 
AGCCTTTGCT TCGTCTGCAG CCACTTTGCT GCAGGGCAGT CCCAAGTCAA AGAGAGAAAT 
GAAGATTTTG TAGAAATAGC ACGAAAACTG AGTTTCCCCA TGGGACGAAT GCTCTTCTCC 
CATGACTATG TATTTTGGTG TGGTGATTTC AACTATCGAA TTGACCTTCC GAATGAAGAG 
GTTAAAGAGC TCATAAGACA ACAAAACTGG GATTCTCTGA TCGCTGGGGA TCAGCTCATC 
AACCAGAAAA ATGCAGGACA GATCTTTAGA GGATTCTTAG AGGGAAAAGT AACGTTTGCT 
CCAACCTATA AATATGACTT GTTTTCTGAA GACTATGACA CCAGTGAGAA GTGTCGCACC 
CCTGCTTGGA CAGACCGTGT CCTCTGGAGA AGGAGGAAGT GGCCTTTTGA CAGATCAGCT 
GAAGATTTAG ATCTCCTGAA TGCTAGTTTC CAAGATGAAA GTAAAATTCT TTATACATGG 
ACCCCTGGCA CCTTGCTGCA CTATGGAAGA GCCGAGCTGA AGACTTCTGA CCATAGGCCC 
GTCGTTGCCT TGATTGACAT AGATATATTT GAAGTTGAAG CTGAAGAGAG ACAAAAAATT 
TATAAAGAAG TTATTGCCGT CCAGGGCCCA CCAGATGGCA CAGTGCTGGT CTCAATCAAA 
AGTTCTGCAC AAGAAAGTAC TTTTTTTGAT GATGCTTTGA TTGATGAGCT TCTGCGGCAG 
TTTGCACACT TTGGTGAAGT TATACTCATA AGATTTGTAG AAGATAAAAT GTGGGTTACA 
TTTTTGGAGG GAAGCTCTGC CTTGAATGCT CTGAGCCTAA ATGGGAAAGA GCTATTAAAT 
CGGACTATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AACATTTGGA AGAAGAGATG 
AGTTTAGAGA AAATCAGTGT CACGTTGCCC TCATCAGCAA GCTCCACCCT GCTTGGTGAA 
GACGCAGAGG TCGCAGCAGA CTTTGACATG GAAGGTGACG TCGATGACTA CAGCGCTGAA 
GTAGAGGAGC TTCTTCCTCA GCATCTGCAG CCGTCCTCAA GTTCTGGCCT GGGCACTTCT 
CCAAGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCCCCCA CAGTACCAGA GTACTCCGCC 
CCTTCTCTTC CCATCAGACC TAGCCGAGCA CCGTCAAGAA CTCCAGGACC TCCAAGTTCG 
CAGGGCTCTC CAGTTGACAC TCAGCCAGCG GCCCAGAAGG ATTCTTCCCA GACTCTAGAG 
CCCAAGAGAC CACCCCCTCC CCGCCCAGTC GCTCCTCCGG CACGTCCTGC CCCACCACAG 
AGACCACCTC CACCTTCAGG GCGTAATCAG CCTTCCCCTC AAGCCGGACT TGCAGGCCCA 
GGACCCGCTG GATACGGTGC GGCTAGACCG ACAATTCCAG CTCGTGCTGG AGTGATCAGC 
GCCCCTCAGA GCCAGGCTCG GGTATGTGCT GGAAGGCCGA CTCCTGACAG CCAAAGCAAG 
CCCTCGGAGA CATTGAAAGG TCCTGCTGTC CTTCCAGAAC CACTGAAGCC TCAGGCTGCA 
TTTCCTCAGC AGCCTTCTCT GCCCACACCT GCTCAAAAGT TACAGGACCC GCTTGTCCCC 
ATAGCAGCAC CTACTATGCC TCCCTCTGGC CCCCAACCAA ATTTGGAAAC CCCCCCACAG 
CCCCCACCTC GGAGCAGGTC ATCTCAAAGC TTGCCTTCAG ACTCCTCACC ACAGCTGCAG 
CAGGAGCAAC CAACAGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172330.1
CDS	630..4850
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	XM_017316841.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316841.1

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GCATCTTCCA AAGTCTTAAA GAACATGTGT 
GAGAACTTCT ACAAATACTC AAAGCCCAAG AAGATCCGAG TGTGTGTTGG CACCTGGAAT 
GTGAACGGCG GGAAGCAGTT CCGCAGCATA GCGTTCAAGA ACCAGACGCT CACAGACTGG 
CTTCTGGACG CTCCCAAGTT AGCCGGCATC CAGGAGTTTC AAGATAAGAG AAGTAAGCCA 
ACTGATATAT TTGCAATTGG CTTTGAAGAA ATGGTGGAGC TGAATGCTGG GAACATTGTG 
AATGCAAGCA CAACCAACCA GAAGCTGTGG GCAGTGGAGC TGCAGAAGAC CATCTCCAGG 
GACAACAAGT ATGTCCTGCT GGCCTCGGAG CAGCTGGTGG GTGTCTGCCT GTTTGTCTTT 
ATCAGACCAC AGCACGCTCC TTTTATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGC 
ATGGGAGGTG CAACTGGAAA CAAGGGCGCC GTTGCAATTC GGATGCTCTT CCACACCACC 
AGCCTTTGCT TCGTCTGCAG CCACTTTGCT GCAGGGCAGT CCCAAGTCAA AGAGAGAAAT 
GAAGATTTTG TAGAAATAGC ACGAAAACTG AGTTTCCCCA TGGGACGAAT GCTCTTCTCC 
CATGACTATG TATTTTGGTG TGGTGATTTC AACTATCGAA TTGACCTTCC GAATGAAGAG 
GTTAAAGAGC TCATAAGACA ACAAAACTGG GATTCTCTGA TCGCTGGGGA TCAGCTCATC 
AACCAGAAAA ATGCAGGACA GATCTTTAGA GGATTCTTAG AGGGAAAAGT AACGTTTGCT 
CCAACCTATA AATATGACTT GTTTTCTGAA GACTATGACA CCAGTGAGAA GTGTCGCACC 
CCTGCTTGGA CAGACCGTGT CCTCTGGAGA AGGAGGAAGT GGCCTTTTGA CAGATCAGCT 
GAAGATTTAG ATCTCCTGAA TGCTAGTTTC CAAGATGAAA GTAAAATTCT TTATACATGG 
ACCCCTGGCA CCTTGCTGCA CTATGGAAGA GCCGAGCTGA AGACTTCTGA CCATAGGCCC 
GTCGTTGCCT TGATTGACAT AGATATATTT GAAGTTGAAG CTGAAGAGAG ACAAAAAATT 
TATAAAGAAG TTATTGCCGT CCAGGGCCCA CCAGATGGCA CAGTGCTGGT CTCAATCAAA 
AGTTCTGCAC AAGAAAGTAC TTTTTTTGAT GATGCTTTGA TTGATGAGCT TCTGCGGCAG 
TTTGCACACT TTGGTGAAGT TATACTCATA AGATTTGTAG AAGATAAAAT GTGGGTTACA 
TTTTTGGAGG GAAGCTCTGC CTTGAATGCT CTGAGCCTAA ATGGGAAAGA GCTATTAAAT 
CGGACTATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AACATTTGGA AGAAGAGATG 
AGTTTAGAGA AAATCAGTGT CACGTTGCCC TCATCAGCAA GCTCCACCCT GCTTGGTGAA 
GACGCAGAGG TCGCAGCAGA CTTTGACATG GAAGGTGACG TCGATGACTA CAGCGCTGAA 
GTAGAGGAGC TTCTTCCTCA GCATCTGCAG CCGTCCTCAA GTTCTGGCCT GGGCACTTCT 
CCAAGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCCCCCA CAGTACCAGA GTACTCCGCC 
CCTTCTCTTC CCATCAGACC TAGCCGAGCA CCGTCAAGAA CTCCAGGACC TCCAAGTTCG 
CAGGGCTCTC CAGTTGACAC TCAGCCAGCG GCCCAGAAGG ATTCTTCCCA GACTCTAGAG 
CCCAAGAGAC CACCCCCTCC CCGCCCAGTC GCTCCTCCGG CACGTCCTGC CCCACCACAG 
AGACCACCTC CACCTTCAGG GCGTAATCAG CCTTCCCCTC AAGCCGGACT TGCAGGCCCA 
GGACCCGCTG GATACGGTGC GGCTAGACCG ACAATTCCAG CTCGTGCTGG AGTGATCAGC 
GCCCCTCAGA GCCAGGCTCG GGTATGTGCT GGAAGGCCGA CTCCTGACAG CCAAAGCAAG 
CCCTCGGAGA CATTGAAAGG TCCTGCTGTC CTTCCAGAAC CACTGAAGCC TCAGGCTGCA 
TTTCCTCAGC AGCCTTCTCT GCCCACACCT GCTCAAAAGT TACAGGACCC GCTTGTCCCC 
ATAGCAGCAC CTACTATGCC TCCCTCTGGC CCCCAACCAA ATTTGGAAAC CCCCCCACAG 
CCCCCACCTC GGAGCAGGTC ATCTCAAAGC TTGCCTTCAG ACTCCTCACC ACAGCTGCAG 
CAGGAGCAAC CAACAGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_017316841.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172330.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X30, mRNA.

Price & Availability↑

CloneID	OMu79244
Clone ID Related Accession (Same CDS sequence)	XM_017316841.1 , XM_017316841.2
Accession Version	XM_017316841.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316841.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GCATCTTCCA AAGTCTTAAA GAACATGTGT 
GAGAACTTCT ACAAATACTC AAAGCCCAAG AAGATCCGAG TGTGTGTTGG CACCTGGAAT 
GTGAACGGCG GGAAGCAGTT CCGCAGCATA GCGTTCAAGA ACCAGACGCT CACAGACTGG 
CTTCTGGACG CTCCCAAGTT AGCCGGCATC CAGGAGTTTC AAGATAAGAG AAGTAAGCCA 
ACTGATATAT TTGCAATTGG CTTTGAAGAA ATGGTGGAGC TGAATGCTGG GAACATTGTG 
AATGCAAGCA CAACCAACCA GAAGCTGTGG GCAGTGGAGC TGCAGAAGAC CATCTCCAGG 
GACAACAAGT ATGTCCTGCT GGCCTCGGAG CAGCTGGTGG GTGTCTGCCT GTTTGTCTTT 
ATCAGACCAC AGCACGCTCC TTTTATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGC 
ATGGGAGGTG CAACTGGAAA CAAGGGCGCC GTTGCAATTC GGATGCTCTT CCACACCACC 
AGCCTTTGCT TCGTCTGCAG CCACTTTGCT GCAGGGCAGT CCCAAGTCAA AGAGAGAAAT 
GAAGATTTTG TAGAAATAGC ACGAAAACTG AGTTTCCCCA TGGGACGAAT GCTCTTCTCC 
CATGACTATG TATTTTGGTG TGGTGATTTC AACTATCGAA TTGACCTTCC GAATGAAGAG 
GTTAAAGAGC TCATAAGACA ACAAAACTGG GATTCTCTGA TCGCTGGGGA TCAGCTCATC 
AACCAGAAAA ATGCAGGACA GATCTTTAGA GGATTCTTAG AGGGAAAAGT AACGTTTGCT 
CCAACCTATA AATATGACTT GTTTTCTGAA GACTATGACA CCAGTGAGAA GTGTCGCACC 
CCTGCTTGGA CAGACCGTGT CCTCTGGAGA AGGAGGAAGT GGCCTTTTGA CAGATCAGCT 
GAAGATTTAG ATCTCCTGAA TGCTAGTTTC CAAGATGAAA GTAAAATTCT TTATACATGG 
ACCCCTGGCA CCTTGCTGCA CTATGGAAGA GCCGAGCTGA AGACTTCTGA CCATAGGCCC 
GTCGTTGCCT TGATTGACAT AGATATATTT GAAGTTGAAG CTGAAGAGAG ACAAAAAATT 
TATAAAGAAG TTATTGCCGT CCAGGGCCCA CCAGATGGCA CAGTGCTGGT CTCAATCAAA 
AGTTCTGCAC AAGAAAGTAC TTTTTTTGAT GATGCTTTGA TTGATGAGCT TCTGCGGCAG 
TTTGCACACT TTGGTGAAGT TATACTCATA AGATTTGTAG AAGATAAAAT GTGGGTTACA 
TTTTTGGAGG GAAGCTCTGC CTTGAATGCT CTGAGCCTAA ATGGGAAAGA GCTATTAAAT 
CGGACTATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AACATTTGGA AGAAGAGATG 
AGTTTAGAGA AAATCAGTGT CACGTTGCCC TCATCAGCAA GCTCCACCCT GCTTGGTGAA 
GACGCAGAGG TCGCAGCAGA CTTTGACATG GAAGGTGACG TCGATGACTA CAGCGCTGAA 
GTAGAGGAGC TTCTTCCTCA GCATCTGCAG CCGTCCTCAA GTTCTGGCCT GGGCACTTCT 
CCAAGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCCCCCA CAGTACCAGA GTACTCCGCC 
CCTTCTCTTC CCATCAGACC TAGCCGAGCA CCGTCAAGAA CTCCAGGACC TCCAAGTTCG 
CAGGGCTCTC CAGTTGACAC TCAGCCAGCG GCCCAGAAGG ATTCTTCCCA GACTCTAGAG 
CCCAAGAGAC CACCCCCTCC CCGCCCAGTC GCTCCTCCGG CACGTCCTGC CCCACCACAG 
AGACCACCTC CACCTTCAGG GCGTAATCAG CCTTCCCCTC AAGCCGGACT TGCAGGCCCA 
GGACCCGCTG GATACGGTGC GGCTAGACCG ACAATTCCAG CTCGTGCTGG AGTGATCAGC 
GCCCCTCAGA GCCAGGCTCG GGTATGTGCT GGAAGGCCGA CTCCTGACAG CCAAAGCAAG 
CCCTCGGAGA CATTGAAAGG TCCTGCTGTC CTTCCAGAAC CACTGAAGCC TCAGGCTGCA 
TTTCCTCAGC AGCCTTCTCT GCCCACACCT GCTCAAAAGT TACAGGACCC GCTTGTCCCC 
ATAGCAGCAC CTACTATGCC TCCCTCTGGC CCCCAACCAA ATTTGGAAAC CCCCCCACAG 
CCCCCACCTC GGAGCAGGTC ATCTCAAAGC TTGCCTTCAG ACTCCTCACC ACAGCTGCAG 
CAGGAGCAAC CAACAGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172330.1
CDS	2226..6446
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	XM_017316841.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316841.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GCATCTTCCA AAGTCTTAAA GAACATGTGT 
GAGAACTTCT ACAAATACTC AAAGCCCAAG AAGATCCGAG TGTGTGTTGG CACCTGGAAT 
GTGAACGGCG GGAAGCAGTT CCGCAGCATA GCGTTCAAGA ACCAGACGCT CACAGACTGG 
CTTCTGGACG CTCCCAAGTT AGCCGGCATC CAGGAGTTTC AAGATAAGAG AAGTAAGCCA 
ACTGATATAT TTGCAATTGG CTTTGAAGAA ATGGTGGAGC TGAATGCTGG GAACATTGTG 
AATGCAAGCA CAACCAACCA GAAGCTGTGG GCAGTGGAGC TGCAGAAGAC CATCTCCAGG 
GACAACAAGT ATGTCCTGCT GGCCTCGGAG CAGCTGGTGG GTGTCTGCCT GTTTGTCTTT 
ATCAGACCAC AGCACGCTCC TTTTATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGC 
ATGGGAGGTG CAACTGGAAA CAAGGGCGCC GTTGCAATTC GGATGCTCTT CCACACCACC 
AGCCTTTGCT TCGTCTGCAG CCACTTTGCT GCAGGGCAGT CCCAAGTCAA AGAGAGAAAT 
GAAGATTTTG TAGAAATAGC ACGAAAACTG AGTTTCCCCA TGGGACGAAT GCTCTTCTCC 
CATGACTATG TATTTTGGTG TGGTGATTTC AACTATCGAA TTGACCTTCC GAATGAAGAG 
GTTAAAGAGC TCATAAGACA ACAAAACTGG GATTCTCTGA TCGCTGGGGA TCAGCTCATC 
AACCAGAAAA ATGCAGGACA GATCTTTAGA GGATTCTTAG AGGGAAAAGT AACGTTTGCT 
CCAACCTATA AATATGACTT GTTTTCTGAA GACTATGACA CCAGTGAGAA GTGTCGCACC 
CCTGCTTGGA CAGACCGTGT CCTCTGGAGA AGGAGGAAGT GGCCTTTTGA CAGATCAGCT 
GAAGATTTAG ATCTCCTGAA TGCTAGTTTC CAAGATGAAA GTAAAATTCT TTATACATGG 
ACCCCTGGCA CCTTGCTGCA CTATGGAAGA GCCGAGCTGA AGACTTCTGA CCATAGGCCC 
GTCGTTGCCT TGATTGACAT AGATATATTT GAAGTTGAAG CTGAAGAGAG ACAAAAAATT 
TATAAAGAAG TTATTGCCGT CCAGGGCCCA CCAGATGGCA CAGTGCTGGT CTCAATCAAA 
AGTTCTGCAC AAGAAAGTAC TTTTTTTGAT GATGCTTTGA TTGATGAGCT TCTGCGGCAG 
TTTGCACACT TTGGTGAAGT TATACTCATA AGATTTGTAG AAGATAAAAT GTGGGTTACA 
TTTTTGGAGG GAAGCTCTGC CTTGAATGCT CTGAGCCTAA ATGGGAAAGA GCTATTAAAT 
CGGACTATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AACATTTGGA AGAAGAGATG 
AGTTTAGAGA AAATCAGTGT CACGTTGCCC TCATCAGCAA GCTCCACCCT GCTTGGTGAA 
GACGCAGAGG TCGCAGCAGA CTTTGACATG GAAGGTGACG TCGATGACTA CAGCGCTGAA 
GTAGAGGAGC TTCTTCCTCA GCATCTGCAG CCGTCCTCAA GTTCTGGCCT GGGCACTTCT 
CCAAGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCCCCCA CAGTACCAGA GTACTCCGCC 
CCTTCTCTTC CCATCAGACC TAGCCGAGCA CCGTCAAGAA CTCCAGGACC TCCAAGTTCG 
CAGGGCTCTC CAGTTGACAC TCAGCCAGCG GCCCAGAAGG ATTCTTCCCA GACTCTAGAG 
CCCAAGAGAC CACCCCCTCC CCGCCCAGTC GCTCCTCCGG CACGTCCTGC CCCACCACAG 
AGACCACCTC CACCTTCAGG GCGTAATCAG CCTTCCCCTC AAGCCGGACT TGCAGGCCCA 
GGACCCGCTG GATACGGTGC GGCTAGACCG ACAATTCCAG CTCGTGCTGG AGTGATCAGC 
GCCCCTCAGA GCCAGGCTCG GGTATGTGCT GGAAGGCCGA CTCCTGACAG CCAAAGCAAG 
CCCTCGGAGA CATTGAAAGG TCCTGCTGTC CTTCCAGAAC CACTGAAGCC TCAGGCTGCA 
TTTCCTCAGC AGCCTTCTCT GCCCACACCT GCTCAAAAGT TACAGGACCC GCTTGTCCCC 
ATAGCAGCAC CTACTATGCC TCCCTCTGGC CCCCAACCAA ATTTGGAAAC CCCCCCACAG 
CCCCCACCTC GGAGCAGGTC ATCTCAAAGC TTGCCTTCAG ACTCCTCACC ACAGCTGCAG 
CAGGAGCAAC CAACAGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_030248907.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104767.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X23, mRNA.

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CloneID	OMu86850
Clone ID Related Accession (Same CDS sequence)	XM_030248907.1
Accession Version	XM_030248907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGGTGTTGG AGCCCCTCCC AGTCCCGGGG TTGCTAGGAG AGAGATAGAA 
GCACCCAAAA GCCCTGGAAC AGCACGGAAA GATAATATAG GGCGTAATCA GCCTTCCCCT 
CAAGCCGGAC TTGCAGGCCC AGGACCCGCT GGATACGGTG CGGCTAGACC GACAATTCCA 
GCTCGTGCTG GAGTGATCAG CGCCCCTCAG AGCCAGGCTC GGGTATGTGC TGGAAGGCCG 
ACTCCTGACA GCCAAAGCAA GCCCTCGGAG ACATTGAAAG GTCCTGCTGT CCTTCCAGAA 
CCACTGAAGC CTCAGGCTGC ATTTCCTCAG CAGCCTTCTC TGCCCACACC TGCTCAAAAG 
TTACAGGACC CGCTTGTCCC CATAGCAGCA CCTACTATGC CTCCCTCTGG CCCCCAACCA 
AATTTGGAAA CCCCCCCACA GCCCCCACCT CGGAGCAGGT CATCTCAAAG CTTGCCTTCA 
GACTCCTCAC CACAGCTGCA GGAGCAACCA ACAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104767.1
CDS	2226..6584
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQEQPTG

Target ORF information:

RefSeq Version	XM_030248907.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248907.1

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGGTGTTGG AGCCCCTCCC AGTCCCGGGG TTGCTAGGAG AGAGATAGAA 
GCACCCAAAA GCCCTGGAAC AGCACGGAAA GATAATATAG GGCGTAATCA GCCTTCCCCT 
CAAGCCGGAC TTGCAGGCCC AGGACCCGCT GGATACGGTG CGGCTAGACC GACAATTCCA 
GCTCGTGCTG GAGTGATCAG CGCCCCTCAG AGCCAGGCTC GGGTATGTGC TGGAAGGCCG 
ACTCCTGACA GCCAAAGCAA GCCCTCGGAG ACATTGAAAG GTCCTGCTGT CCTTCCAGAA 
CCACTGAAGC CTCAGGCTGC ATTTCCTCAG CAGCCTTCTC TGCCCACACC TGCTCAAAAG 
TTACAGGACC CGCTTGTCCC CATAGCAGCA CCTACTATGC CTCCCTCTGG CCCCCAACCA 
AATTTGGAAA CCCCCCCACA GCCCCCACCT CGGAGCAGGT CATCTCAAAG CTTGCCTTCA 
GACTCCTCAC CACAGCTGCA GGAGCAACCA ACAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_030248908.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104768.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X25, mRNA.

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CloneID	OMu86851
Clone ID Related Accession (Same CDS sequence)	XM_030248908.1
Accession Version	XM_030248908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGCACCCAA AAGCCCTGGA 
ACAGCACGGA AAGATAATAT AGGGCGTAAT CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC 
CCAGGACCCG CTGGATACGG TGCGGCTAGA CCGACAATTC CAGCTCGTGC TGGAGTGATC 
AGCGCCCCTC AGAGCCAGGC TCGGGTATGT GCTGGAAGGC CGACTCCTGA CAGCCAAAGC 
AAGCCCTCGG AGACATTGAA AGGTCCTGCT GTCCTTCCAG AACCACTGAA GCCTCAGGCT 
GCATTTCCTC AGCAGCCTTC TCTGCCCACA CCTGCTCAAA AGTTACAGGA CCCGCTTGTC 
CCCATAGCAG CACCTACTAT GCCTCCCTCT GGCCCCCAAC CAAATTTGGA AACCCCCCCA 
CAGCCCCCAC CTCGGAGCAG GTCATCTCAA AGCTTGCCTT CAGACTCCTC ACCACAGCTG 
CAGCAGGAGC AACCAACAGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104768.1
CDS	2226..6509
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	XM_030248908.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248908.1

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGCACCCAA AAGCCCTGGA 
ACAGCACGGA AAGATAATAT AGGGCGTAAT CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC 
CCAGGACCCG CTGGATACGG TGCGGCTAGA CCGACAATTC CAGCTCGTGC TGGAGTGATC 
AGCGCCCCTC AGAGCCAGGC TCGGGTATGT GCTGGAAGGC CGACTCCTGA CAGCCAAAGC 
AAGCCCTCGG AGACATTGAA AGGTCCTGCT GTCCTTCCAG AACCACTGAA GCCTCAGGCT 
GCATTTCCTC AGCAGCCTTC TCTGCCCACA CCTGCTCAAA AGTTACAGGA CCCGCTTGTC 
CCCATAGCAG CACCTACTAT GCCTCCCTCT GGCCCCCAAC CAAATTTGGA AACCCCCCCA 
CAGCCCCCAC CTCGGAGCAG GTCATCTCAA AGCTTGCCTT CAGACTCCTC ACCACAGCTG 
CAGCAGGAGC AACCAACAGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_030248909.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104769.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OMu86852
Clone ID Related Accession (Same CDS sequence)	XM_030248909.1
Accession Version	XM_030248909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGGCGTAAT CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC 
CCAGGACCCG CTGGATACGG TGCGGCTAGA CCGACAATTC CAGCTCGTGC TGGAGTGATC 
AGCGCCCCTC AGAGCCAGGC TCGGGTATGT GCTGGAAGGC CGACTCCTGA CAGCCAAAGC 
AAGCCCTCGG AGACATTGAA AGGTCCTGCT GTCCTTCCAG AACCACTGAA GCCTCAGGCT 
GCATTTCCTC AGCAGCCTTC TCTGCCCACA CCTGCTCAAA AGTTACAGGA CCCGCTTGTC 
CCCATAGCAG CACCTACTAT GCCTCCCTCT GGCCCCCAAC CAAATTTGGA AACCCCCCCA 
CAGCCCCCAC CTCGGAGCAG GTCATCTCAA AGCTTGCCTT CAGACTCCTC ACCACAGCTG 
CAGCAGGAGC AACCAACAGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104769.1
CDS	2226..6509
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	XM_030248909.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248909.1

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGGCGTAAT CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC 
CCAGGACCCG CTGGATACGG TGCGGCTAGA CCGACAATTC CAGCTCGTGC TGGAGTGATC 
AGCGCCCCTC AGAGCCAGGC TCGGGTATGT GCTGGAAGGC CGACTCCTGA CAGCCAAAGC 
AAGCCCTCGG AGACATTGAA AGGTCCTGCT GTCCTTCCAG AACCACTGAA GCCTCAGGCT 
GCATTTCCTC AGCAGCCTTC TCTGCCCACA CCTGCTCAAA AGTTACAGGA CCCGCTTGTC 
CCCATAGCAG CACCTACTAT GCCTCCCTCT GGCCCCCAAC CAAATTTGGA AACCCCCCCA 
CAGCCCCCAC CTCGGAGCAG GTCATCTCAA AGCTTGCCTT CAGACTCCTC ACCACAGCTG 
CAGCAGGAGC AACCAACAGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_030248910.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104770.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OMu86853
Clone ID Related Accession (Same CDS sequence)	XM_030248910.1
Accession Version	XM_030248910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGCGTAA TCAGCCTTCC 
CCTCAAGCCG GACTTGCAGG CCCAGGACCC GCTGGATACG GTGCGGCTAG ACCGACAATT 
CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT CAGAGCCAGG CTCGGGTATG TGCTGGAAGG 
CCGACTCCTG ACAGCCAAAG CAAGCCCTCG GAGACATTGA AAGGTCCTGC TGTCCTTCCA 
GAACCACTGA AGCCTCAGGC TGCATTTCCT CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA 
AAGTTACAGG ACCCGCTTGT CCCCATAGCA GCACCTACTA TGCCTCCCTC TGGCCCCCAA 
CCAAATTTGG AAACCCCCCC ACAGCCCCCA CCTCGGAGCA GGTCATCTCA AAGCTTGCCT 
TCAGACTCCT CACCACAGCT GCAGGAGCAA CCAACAGGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104770.1
CDS	2226..6467
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQEQPTG

Target ORF information:

RefSeq Version	XM_030248910.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248910.1

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGCGTAA TCAGCCTTCC 
CCTCAAGCCG GACTTGCAGG CCCAGGACCC GCTGGATACG GTGCGGCTAG ACCGACAATT 
CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT CAGAGCCAGG CTCGGGTATG TGCTGGAAGG 
CCGACTCCTG ACAGCCAAAG CAAGCCCTCG GAGACATTGA AAGGTCCTGC TGTCCTTCCA 
GAACCACTGA AGCCTCAGGC TGCATTTCCT CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA 
AAGTTACAGG ACCCGCTTGT CCCCATAGCA GCACCTACTA TGCCTCCCTC TGGCCCCCAA 
CCAAATTTGG AAACCCCCCC ACAGCCCCCA CCTCGGAGCA GGTCATCTCA AAGCTTGCCT 
TCAGACTCCT CACCACAGCT GCAGGAGCAA CCAACAGGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( NM_001045515.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001038980.1 Mus musculus synaptojanin 1 (Synj1), transcript variant 2, mRNA.

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CloneID	OMu12395
Clone ID Related Accession (Same CDS sequence)	NM_001045515.1
Accession Version	NM_001045515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ104552.1, AB093272.1 and CJ048086.1. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, uses a downstream start codon, and includes an alternate exon in the 3' coding region resulting in a frameshift, compared to variant 1. The resulting isoform (b) has a shorter N-terminus and a shorter and distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTTCA GCAAAGGATT TCGAATCTAC CACAAATTGG ATCCCCCACC GTTCAGCCTC 
ATAGTGGAAA CTAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTGGCTGTG 
CTCTCATCTG CAGAAAAGGA GGCTATTAAA GGCACATATG CCAAAGTACT GGATGCATAC 
GGACTTTTGG GAGTTTTACG ATTAAATCTT GGTGATACCA TGCTGCACTA TCTGGTCCTA 
GTCACTGGAT GCATGTCTGT GGGGAAAATC CAAGAATCTG AAGTTTTCCG AGTTACTTCC 
ACTGAGTTTA TATCATTGCG AGTTGATGCT TCAGATGAGG ACCGCATTTC GGAAGTACGA 
AAAGTTTTGA ACTCGGGAAA CTTTTATTTT GCATGGTCTG CATCTGGAGT CAGCTTAGAT 
CTGAGTCTGA ATGCACATCG AAGCATGCAA GAGCACACAA CCGACAATAG GTTTTTCTGG 
AATCAGTCTC TGCATTTGCA TCTCAAGCAC TATGGTGTGA ACTGCGATGA CTGGTTATTA 
CGGCTCATGT GTGGGGGAGT AGAGATTAGA ACCATTTATG CTGCCCATAA ACAGGCAAAA 
GCTTGCCTCA TCTCAAGACT AAGCTGTGAA CGAGCTGGGA CAAGGTTCAA TGTCCGGGGA 
ACCAACGATG ATGGTCATGT TGCCAACTTT GTAGAGACAG AGCAGGTTAT ATACTTAGAT 
GACTGTGTCT CTTCCTTCAT ACAAATCCGA GGATCTGTTC CATTGTTCTG GGAGCAACCA 
GGGTTGCAAG TGGGATCCCA TCGTGTTCGT ATGTCAAGGG GATTTGAAGC CAATGCGCCT 
GCCTTTGACA GGCATTTCCG AACACTTAAG GACTTATATG GTAAACAGAT AGTAGTGAAT 
CTGCTTGGGT CTAAGGAAGG CGAGCATATG CTGAGCAAGG CTTTCCAGAG TCATTTAAAA 
GCCTCTGAAC ACGCTTCTGA TATCCACATG GTGTCTTTTG ACTATCATCA AATGGTTAAA 
GGAGGAAAGG CAGAAAAACT ACACAGTATT CTCAAACCAC AAGTCCAGAA GTTTCTAGAT 
TATGGATTTT TTTATTTCGA TGGCAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA 
ACAAACTGCT TGGATTGTCT GGATAGAACA AATAGTGTGC AGGCATTCCT TGGCTTAGAG 
ATGCTCGCTA AGCAGCTGGA AGCTCTGGGC TTGGCTGAGA AGCCTCAGCT GGTGACCCGC 
TTCCAAGAAG TTTTCCGGTC CATGTGGTCT GTGAATGGCG ACTCAATCAG TAAAATATAT 
GCAGGCACCG GGGCCCTGGA AGGGAAGGCC AAGTTAAAAG ATGGTGCGCG ATCTGTCACC 
AGAACCATCC AGAATAACTT CTTCGACAGC TCCAAGCAGG AAGCCATTGA TGTCCTGCTC 
CTGGGAAATA CTCTCAACAG CGATTTAGCT GACAAAGCCC GAGCGCTTCT AACTACTGGA 
AGTTTGCGTG TTTCTGAACA GACATTACAG TCAGCATCTT CCAAAGTCTT AAAGAACATG 
TGTGAGAACT TCTACAAATA CTCAAAGCCC AAGAAGATCC GAGTGTGTGT TGGCACCTGG 
AATGTGAACG GCGGGAAGCA GTTCCGCAGC ATAGCGTTCA AGAACCAGAC GCTCACAGAC 
TGGCTTCTGG ACGCTCCCAA GTTAGCCGGC ATCCAGGAGT TTCAAGATAA GAGAAGTAAG 
CCAACTGATA TATTTGCAAT TGGCTTTGAA GAAATGGTGG AGCTGAATGC TGGGAACATT 
GTGAATGCAA GCACAACCAA CCAGAAGCTG TGGGCAGTGG AGCTGCAGAA GACCATCTCC 
AGGGACAACA AGTATGTCCT GCTGGCCTCG GAGCAGCTGG TGGGTGTCTG CCTGTTTGTC 
TTTATCAGAC CACAGCACGC TCCTTTTATC AGGGATGTTG CAGTTGATAC TGTGAAAACT 
GGCATGGGAG GTGCAACTGG AAACAAGGGC GCCGTTGCAA TTCGGATGCT CTTCCACACC 
ACCAGCCTTT GCTTCGTCTG CAGCCACTTT GCTGCAGGGC AGTCCCAAGT CAAAGAGAGA 
AATGAAGATT TTGTAGAAAT AGCACGAAAA CTGAGTTTCC CCATGGGACG AATGCTCTTC 
TCCCATGACT ATGTATTTTG GTGTGGTGAT TTCAACTATC GAATTGACCT TCCGAATGAA 
GAGGTTAAAG AGCTCATAAG ACAACAAAAC TGGGATTCTC TGATCGCTGG GGATCAGCTC 
ATCAACCAGA AAAATGCAGG ACAGATCTTT AGAGGATTCT TAGAGGGAAA AGTAACGTTT 
GCTCCAACCT ATAAATATGA CTTGTTTTCT GAAGACTATG ACACCAGTGA GAAGTGTCGC 
ACCCCTGCTT GGACAGACCG TGTCCTCTGG AGAAGGAGGA AGTGGCCTTT TGACAGATCA 
GCTGAAGATT TAGATCTCCT GAATGCTAGT TTCCAAGATG AAAGTAAAAT TCTTTATACA 
TGGACCCCTG GCACCTTGCT GCACTATGGA AGAGCCGAGC TGAAGACTTC TGACCATAGG 
CCCGTCGTTG CCTTGATTGA CATAGATATA TTTGAAGTTG AAGCTGAAGA GAGACAAAAA 
ATTTATAAAG AAGTTATTGC CGTCCAGGGC CCACCAGATG GCACAGTGCT GGTCTCAATC 
AAAAGTTCTG CACAAGAAAG TACTTTTTTT GATGATGCTT TGATTGATGA GCTTCTGCGG 
CAGTTTGCAC ACTTTGGTGA AGTTATACTC ATAAGATTTG TAGAAGATAA AATGTGGGTT 
ACATTTTTGG AGGGAAGCTC TGCCTTGAAT GCTCTGAGCC TAAATGGGAA AGAGCTATTA 
AATCGGACTA TAACAATTAC TTTAAAAAGT CCAGACTGGA TCAAACATTT GGAAGAAGAG 
ATGAGTTTAG AGAAAATCAG TGTCACGTTG CCCTCATCAG CAAGCTCCAC CCTGCTTGGT 
GAAGACGCAG AGGTCGCAGC AGACTTTGAC ATGGAAGGTG ACGTCGATGA CTACAGCGCT 
GAAGTAGAGG AGCTTCTTCC TCAGCATCTG CAGCCGTCCT CAAGTTCTGG CCTGGGCACT 
TCTCCAAGTT CTTCACCCCG GACCAGTCCC TGCCAGTCCC CCACAGTACC AGAGTACTCC 
GCCCCTTCTC TTCCCATCAG ACCTAGCCGA GCACCGTCAA GAACTCCAGG ACCTCCAAGT 
TCGCAGGGCT CTCCAGTTGA CACTCAGCCA GCGGCCCAGA AGGATTCTTC CCAGACTCTA 
GAGCCCAAGA GACCACCCCC TCCCCGCCCA GTCGCTCCTC CGGCACGTCC TGCCCCACCA 
CAGAGACCAC CTCCACCTTC AGGGGCTAGG AGTCCTGCGC CTGCTAGAAA AGAATTTGGA 
GGTGTTGGAG CCCCTCCCAG TCCCGGGGTT GCTAGGAGAG AGATAGAAGC ACCCAAAAGC 
CCTGGAACAG CACGGAAAGA TAATATAGGG CGTAATCAGC CTTCCCCTCA AGCCGGACTT 
GCAGGCCCAG GACCCGCTGG ATACGGTGCG GCTAGACCGA CAATTCCAGC TCGTGCTGGA 
GTGATCAGCG CCCCTCAGAG CCAGGCTCGG GTATGTGCTG GAAGGCCGAC TCCTGACAGC 
CAAAGCAAGC CCTCGGAGAC ATTGAAAGGT CCTGCTGTCC TTCCAGAACC ACTGAAGCCT 
CAGGCTGCAT TTCCTCAGCA GCCTTCTCTG CCCACACCTG CTCAAAAGTT ACAGGACCCG 
CTTGTCCCCA TAGCAGCACC TACTATGCCT CCCTCTGGCC CCCAACCAAA TTTGGAAACC 
CCCCCACAGC CCCCACCTCG GAGCAGGTCA TCTCAAAGCT TGCCTTCAGA CTCCTCACCA 
CAGCTGCAGC AGGAGCAACC AACAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038980.1
CDS	146..4075
Translation	MAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	NM_001045515.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045515.1

ORF Insert Sequence:

ATGGCGTTCA GCAAAGGATT TCGAATCTAC CACAAATTGG ATCCCCCACC GTTCAGCCTC 
ATAGTGGAAA CTAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTGGCTGTG 
CTCTCATCTG CAGAAAAGGA GGCTATTAAA GGCACATATG CCAAAGTACT GGATGCATAC 
GGACTTTTGG GAGTTTTACG ATTAAATCTT GGTGATACCA TGCTGCACTA TCTGGTCCTA 
GTCACTGGAT GCATGTCTGT GGGGAAAATC CAAGAATCTG AAGTTTTCCG AGTTACTTCC 
ACTGAGTTTA TATCATTGCG AGTTGATGCT TCAGATGAGG ACCGCATTTC GGAAGTACGA 
AAAGTTTTGA ACTCGGGAAA CTTTTATTTT GCATGGTCTG CATCTGGAGT CAGCTTAGAT 
CTGAGTCTGA ATGCACATCG AAGCATGCAA GAGCACACAA CCGACAATAG GTTTTTCTGG 
AATCAGTCTC TGCATTTGCA TCTCAAGCAC TATGGTGTGA ACTGCGATGA CTGGTTATTA 
CGGCTCATGT GTGGGGGAGT AGAGATTAGA ACCATTTATG CTGCCCATAA ACAGGCAAAA 
GCTTGCCTCA TCTCAAGACT AAGCTGTGAA CGAGCTGGGA CAAGGTTCAA TGTCCGGGGA 
ACCAACGATG ATGGTCATGT TGCCAACTTT GTAGAGACAG AGCAGGTTAT ATACTTAGAT 
GACTGTGTCT CTTCCTTCAT ACAAATCCGA GGATCTGTTC CATTGTTCTG GGAGCAACCA 
GGGTTGCAAG TGGGATCCCA TCGTGTTCGT ATGTCAAGGG GATTTGAAGC CAATGCGCCT 
GCCTTTGACA GGCATTTCCG AACACTTAAG GACTTATATG GTAAACAGAT AGTAGTGAAT 
CTGCTTGGGT CTAAGGAAGG CGAGCATATG CTGAGCAAGG CTTTCCAGAG TCATTTAAAA 
GCCTCTGAAC ACGCTTCTGA TATCCACATG GTGTCTTTTG ACTATCATCA AATGGTTAAA 
GGAGGAAAGG CAGAAAAACT ACACAGTATT CTCAAACCAC AAGTCCAGAA GTTTCTAGAT 
TATGGATTTT TTTATTTCGA TGGCAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA 
ACAAACTGCT TGGATTGTCT GGATAGAACA AATAGTGTGC AGGCATTCCT TGGCTTAGAG 
ATGCTCGCTA AGCAGCTGGA AGCTCTGGGC TTGGCTGAGA AGCCTCAGCT GGTGACCCGC 
TTCCAAGAAG TTTTCCGGTC CATGTGGTCT GTGAATGGCG ACTCAATCAG TAAAATATAT 
GCAGGCACCG GGGCCCTGGA AGGGAAGGCC AAGTTAAAAG ATGGTGCGCG ATCTGTCACC 
AGAACCATCC AGAATAACTT CTTCGACAGC TCCAAGCAGG AAGCCATTGA TGTCCTGCTC 
CTGGGAAATA CTCTCAACAG CGATTTAGCT GACAAAGCCC GAGCGCTTCT AACTACTGGA 
AGTTTGCGTG TTTCTGAACA GACATTACAG TCAGCATCTT CCAAAGTCTT AAAGAACATG 
TGTGAGAACT TCTACAAATA CTCAAAGCCC AAGAAGATCC GAGTGTGTGT TGGCACCTGG 
AATGTGAACG GCGGGAAGCA GTTCCGCAGC ATAGCGTTCA AGAACCAGAC GCTCACAGAC 
TGGCTTCTGG ACGCTCCCAA GTTAGCCGGC ATCCAGGAGT TTCAAGATAA GAGAAGTAAG 
CCAACTGATA TATTTGCAAT TGGCTTTGAA GAAATGGTGG AGCTGAATGC TGGGAACATT 
GTGAATGCAA GCACAACCAA CCAGAAGCTG TGGGCAGTGG AGCTGCAGAA GACCATCTCC 
AGGGACAACA AGTATGTCCT GCTGGCCTCG GAGCAGCTGG TGGGTGTCTG CCTGTTTGTC 
TTTATCAGAC CACAGCACGC TCCTTTTATC AGGGATGTTG CAGTTGATAC TGTGAAAACT 
GGCATGGGAG GTGCAACTGG AAACAAGGGC GCCGTTGCAA TTCGGATGCT CTTCCACACC 
ACCAGCCTTT GCTTCGTCTG CAGCCACTTT GCTGCAGGGC AGTCCCAAGT CAAAGAGAGA 
AATGAAGATT TTGTAGAAAT AGCACGAAAA CTGAGTTTCC CCATGGGACG AATGCTCTTC 
TCCCATGACT ATGTATTTTG GTGTGGTGAT TTCAACTATC GAATTGACCT TCCGAATGAA 
GAGGTTAAAG AGCTCATAAG ACAACAAAAC TGGGATTCTC TGATCGCTGG GGATCAGCTC 
ATCAACCAGA AAAATGCAGG ACAGATCTTT AGAGGATTCT TAGAGGGAAA AGTAACGTTT 
GCTCCAACCT ATAAATATGA CTTGTTTTCT GAAGACTATG ACACCAGTGA GAAGTGTCGC 
ACCCCTGCTT GGACAGACCG TGTCCTCTGG AGAAGGAGGA AGTGGCCTTT TGACAGATCA 
GCTGAAGATT TAGATCTCCT GAATGCTAGT TTCCAAGATG AAAGTAAAAT TCTTTATACA 
TGGACCCCTG GCACCTTGCT GCACTATGGA AGAGCCGAGC TGAAGACTTC TGACCATAGG 
CCCGTCGTTG CCTTGATTGA CATAGATATA TTTGAAGTTG AAGCTGAAGA GAGACAAAAA 
ATTTATAAAG AAGTTATTGC CGTCCAGGGC CCACCAGATG GCACAGTGCT GGTCTCAATC 
AAAAGTTCTG CACAAGAAAG TACTTTTTTT GATGATGCTT TGATTGATGA GCTTCTGCGG 
CAGTTTGCAC ACTTTGGTGA AGTTATACTC ATAAGATTTG TAGAAGATAA AATGTGGGTT 
ACATTTTTGG AGGGAAGCTC TGCCTTGAAT GCTCTGAGCC TAAATGGGAA AGAGCTATTA 
AATCGGACTA TAACAATTAC TTTAAAAAGT CCAGACTGGA TCAAACATTT GGAAGAAGAG 
ATGAGTTTAG AGAAAATCAG TGTCACGTTG CCCTCATCAG CAAGCTCCAC CCTGCTTGGT 
GAAGACGCAG AGGTCGCAGC AGACTTTGAC ATGGAAGGTG ACGTCGATGA CTACAGCGCT 
GAAGTAGAGG AGCTTCTTCC TCAGCATCTG CAGCCGTCCT CAAGTTCTGG CCTGGGCACT 
TCTCCAAGTT CTTCACCCCG GACCAGTCCC TGCCAGTCCC CCACAGTACC AGAGTACTCC 
GCCCCTTCTC TTCCCATCAG ACCTAGCCGA GCACCGTCAA GAACTCCAGG ACCTCCAAGT 
TCGCAGGGCT CTCCAGTTGA CACTCAGCCA GCGGCCCAGA AGGATTCTTC CCAGACTCTA 
GAGCCCAAGA GACCACCCCC TCCCCGCCCA GTCGCTCCTC CGGCACGTCC TGCCCCACCA 
CAGAGACCAC CTCCACCTTC AGGGGCTAGG AGTCCTGCGC CTGCTAGAAA AGAATTTGGA 
GGTGTTGGAG CCCCTCCCAG TCCCGGGGTT GCTAGGAGAG AGATAGAAGC ACCCAAAAGC 
CCTGGAACAG CACGGAAAGA TAATATAGGG CGTAATCAGC CTTCCCCTCA AGCCGGACTT 
GCAGGCCCAG GACCCGCTGG ATACGGTGCG GCTAGACCGA CAATTCCAGC TCGTGCTGGA 
GTGATCAGCG CCCCTCAGAG CCAGGCTCGG GTATGTGCTG GAAGGCCGAC TCCTGACAGC 
CAAAGCAAGC CCTCGGAGAC ATTGAAAGGT CCTGCTGTCC TTCCAGAACC ACTGAAGCCT 
CAGGCTGCAT TTCCTCAGCA GCCTTCTCTG CCCACACCTG CTCAAAAGTT ACAGGACCCG 
CTTGTCCCCA TAGCAGCACC TACTATGCCT CCCTCTGGCC CCCAACCAAA TTTGGAAACC 
CCCCCACAGC CCCCACCTCG GAGCAGGTCA TCTCAAAGCT TGCCTTCAGA CTCCTCACCA 
CAGCTGCAGC AGGAGCAACC AACAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_006522846.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522909.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OMu52623
Clone ID Related Accession (Same CDS sequence)	XM_006522846.2 , XM_006522846.3
Accession Version	XM_006522846.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006522846.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGAGA ACTTCTACAA ATACTCAAAG CCCAAGAAGA TCCGAGTGTG TGTTGGCACC 
TGGAATGTGA ACGGCGGGAA GCAGTTCCGC AGCATAGCGT TCAAGAACCA GACGCTCACA 
GACTGGCTTC TGGACGCTCC CAAGTTAGCC GGCATCCAGG AGTTTCAAGA TAAGAGAAGT 
AAGCCAACTG ATATATTTGC AATTGGCTTT GAAGAAATGG TGGAGCTGAA TGCTGGGAAC 
ATTGTGAATG CAAGCACAAC CAACCAGAAG CTGTGGGCAG TGGAGCTGCA GAAGACCATC 
TCCAGGGACA ACAAGTATGT CCTGCTGGCC TCGGAGCAGC TGGTGGGTGT CTGCCTGTTT 
GTCTTTATCA GACCACAGCA CGCTCCTTTT ATCAGGGATG TTGCAGTTGA TACTGTGAAA 
ACTGGCATGG GAGGTGCAAC TGGAAACAAG GGCGCCGTTG CAATTCGGAT GCTCTTCCAC 
ACCACCAGCC TTTGCTTCGT CTGCAGCCAC TTTGCTGCAG GGCAGTCCCA AGTCAAAGAG 
AGAAATGAAG ATTTTGTAGA AATAGCACGA AAACTGAGTT TCCCCATGGG ACGAATGCTC 
TTCTCCCATG ACTATGTATT TTGGTGTGGT GATTTCAACT ATCGAATTGA CCTTCCGAAT 
GAAGAGGTTA AAGAGCTCAT AAGACAACAA AACTGGGATT CTCTGATCGC TGGGGATCAG 
CTCATCAACC AGAAAAATGC AGGACAGATC TTTAGAGGAT TCTTAGAGGG AAAAGTAACG 
TTTGCTCCAA CCTATAAATA TGACTTGTTT TCTGAAGACT ATGACACCAG TGAGAAGTGT 
CGCACCCCTG CTTGGACAGA CCGTGTCCTC TGGAGAAGGA GGAAGTGGCC TTTTGACAGA 
TCAGCTGAAG ATTTAGATCT CCTGAATGCT AGTTTCCAAG ATGAAAGTAA AATTCTTTAT 
ACATGGACCC CTGGCACCTT GCTGCACTAT GGAAGAGCCG AGCTGAAGAC TTCTGACCAT 
AGGCCCGTCG TTGCCTTGAT TGACATAGAT ATATTTGAAG TTGAAGCTGA AGAGAGACAA 
AAAATTTATA AAGAAGTTAT TGCCGTCCAG GGCCCACCAG ATGGCACAGT GCTGGTCTCA 
ATCAAAAGTT CTGCACAAGA AAGTACTTTT TTTGATGATG CTTTGATTGA TGAGCTTCTG 
CGGCAGTTTG CACACTTTGG TGAAGTTATA CTCATAAGAT TTGTAGAAGA TAAAATGTGG 
GTTACATTTT TGGAGGGAAG CTCTGCCTTG AATGCTCTGA GCCTAAATGG GAAAGAGCTA 
TTAAATCGGA CTATAACAAT TACTTTAAAA AGTCCAGACT GGATCAAACA TTTGGAAGAA 
GAGATGAGTT TAGAGAAAAT CAGTGTCACG TTGCCCTCAT CAGCAAGCTC CACCCTGCTT 
GGTGAAGACG CAGAGGTCGC AGCAGACTTT GACATGGAAG GTGACGTCGA TGACTACAGC 
GCTGAAGTAG AGGAGCTTCT TCCTCAGCAT CTGCAGCCGT CCTCAAGTTC TGGCCTGGGC 
ACTTCTCCAA GTTCTTCACC CCGGACCAGT CCCTGCCAGT CCCCCACAGT ACCAGAGTAC 
TCCGCCCCTT CTCTTCCCAT CAGACCTAGC CGAGCACCGT CAAGAACTCC AGGACCTCCA 
AGTTCGCAGG GCTCTCCAGT TGACACTCAG CCAGCGGCCC AGAAGGATTC TTCCCAGACT 
CTAGAGCCCA AGAGACCACC CCCTCCCCGC CCAGTCGCTC CTCCGGCACG TCCTGCCCCA 
CCACAGAGAC CACCTCCACC TTCAGGGGCT AGGAGTCCTG CGCCTGCTAG AAAAGAATTT 
GGAGGTGTTG GAGCCCCTCC CAGTCCCGGG GTTGCTAGGA GAGAGATAGA AGCACCCAAA 
AGCCCTGGAA CAGCACGGAA AGATAATATA GGGCGTAATC AGCCTTCCCC TCAAGCCGGA 
CTTGCAGGCC CAGGACCCGC TGGATACGGT GCGGCTAGAC CGACAATTCC AGCTCGTGCT 
GGAGTGATCA GCGCCCCTCA GAGCCAGGCT CGGGTATGTG CTGGAAGGCC GACTCCTGAC 
AGCCAAAGCA AGCCCTCGGA GACATTGAAA GGTCCTGCTG TCCTTCCAGA ACCACTGAAG 
CCTCAGGCTG CATTTCCTCA GCAGCCTTCT CTGCCCACAC CTGCTCAAAA GTTACAGGAC 
CCGCTTGTCC CCATAGCAGC ACCTACTATG CCTCCCTCTG GCCCCCAACC AAATTTGGAA 
ACCCCCCCAC AGCCCCCACC TCGGAGCAGG TCATCTCAAA GCTTGCCTTC AGACTCCTCA 
CCACAGCTGC AGGTAAAAAT AAATGGCATT TCTGGTGTCA AACAAGAGCC AACATTGAAG 
AGTGACCCAT TTGAAGATCT CTCACTTAGT GTGCTTGCTG TATCAAAGGC TCAGCCTTCT 
GTTCAGATTT CACCTGTTCT AACCCCAGAC CCAAAGATGT TGATTCAGTT GCCTTCTGCA 
TCGCAAAGTC AAGTTAACCC TTTGAGTTCT GTAAGTTGCA TGCCAACCAG GCCTCCAGGT 
CCAGAGGAAA GCAAGTCACA GGAGAGTATG GGCAGTTCTG CAAACCCGTT TCCCAGCCTG 
CCCTGCCGGA ACCCTTTTAC AGACAGGACT GCTGCCCCTG GAAACCCATT TAGAGTTCAG 
TCCCAAGAAT CAGAGGCAAC TTCTTGGCTC TCCAAAGAAG AGCCTGTTCC CAACAGTCCC 
TTCCCTCCTC TCATGCCTCT CAGTCATGAC ACGAGCAAGG CTTCAAGTTC GCTTGGTGGC 
TTTGAGGACA ATTTTGATCT GCAGAGCCAG TCTACAGTAA AAACAAGCAA CCCCAAAGGA 
TGGGTAACCT TTGACGAAGA CGACAACTTT CCTACAACAG GAAAGTCAAA GTCAGTTTGT 
CCAGACTTAG TGGGTAACGC GCCAGCTTCA TTTGATGATG ACTGGAGTAA AGGTGCGAGC 
GTTTCTTTCT GTGTGTTGCC AGCCAGAAGG CCACCACCAC CACCACCTCC TGTCCCTCTG 
CTACCACCTG GCACAACCTC ATCTGCAGGA CCTAGCACAA CCCTGCCATC TAAGGCACCT 
TCCACATTGG ACTTCACGGA AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522909.1
CDS	399..3545
Translation	MCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_006522846.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522846.2

ORF Insert Sequence:

ATGTGTGAGA ACTTCTACAA ATACTCAAAG CCCAAGAAGA TCCGAGTGTG TGTTGGCACC 
TGGAATGTGA ACGGCGGGAA GCAGTTCCGC AGCATAGCGT TCAAGAACCA GACGCTCACA 
GACTGGCTTC TGGACGCTCC CAAGTTAGCC GGCATCCAGG AGTTTCAAGA TAAGAGAAGT 
AAGCCAACTG ATATATTTGC AATTGGCTTT GAAGAAATGG TGGAGCTGAA TGCTGGGAAC 
ATTGTGAATG CAAGCACAAC CAACCAGAAG CTGTGGGCAG TGGAGCTGCA GAAGACCATC 
TCCAGGGACA ACAAGTATGT CCTGCTGGCC TCGGAGCAGC TGGTGGGTGT CTGCCTGTTT 
GTCTTTATCA GACCACAGCA CGCTCCTTTT ATCAGGGATG TTGCAGTTGA TACTGTGAAA 
ACTGGCATGG GAGGTGCAAC TGGAAACAAG GGCGCCGTTG CAATTCGGAT GCTCTTCCAC 
ACCACCAGCC TTTGCTTCGT CTGCAGCCAC TTTGCTGCAG GGCAGTCCCA AGTCAAAGAG 
AGAAATGAAG ATTTTGTAGA AATAGCACGA AAACTGAGTT TCCCCATGGG ACGAATGCTC 
TTCTCCCATG ACTATGTATT TTGGTGTGGT GATTTCAACT ATCGAATTGA CCTTCCGAAT 
GAAGAGGTTA AAGAGCTCAT AAGACAACAA AACTGGGATT CTCTGATCGC TGGGGATCAG 
CTCATCAACC AGAAAAATGC AGGACAGATC TTTAGAGGAT TCTTAGAGGG AAAAGTAACG 
TTTGCTCCAA CCTATAAATA TGACTTGTTT TCTGAAGACT ATGACACCAG TGAGAAGTGT 
CGCACCCCTG CTTGGACAGA CCGTGTCCTC TGGAGAAGGA GGAAGTGGCC TTTTGACAGA 
TCAGCTGAAG ATTTAGATCT CCTGAATGCT AGTTTCCAAG ATGAAAGTAA AATTCTTTAT 
ACATGGACCC CTGGCACCTT GCTGCACTAT GGAAGAGCCG AGCTGAAGAC TTCTGACCAT 
AGGCCCGTCG TTGCCTTGAT TGACATAGAT ATATTTGAAG TTGAAGCTGA AGAGAGACAA 
AAAATTTATA AAGAAGTTAT TGCCGTCCAG GGCCCACCAG ATGGCACAGT GCTGGTCTCA 
ATCAAAAGTT CTGCACAAGA AAGTACTTTT TTTGATGATG CTTTGATTGA TGAGCTTCTG 
CGGCAGTTTG CACACTTTGG TGAAGTTATA CTCATAAGAT TTGTAGAAGA TAAAATGTGG 
GTTACATTTT TGGAGGGAAG CTCTGCCTTG AATGCTCTGA GCCTAAATGG GAAAGAGCTA 
TTAAATCGGA CTATAACAAT TACTTTAAAA AGTCCAGACT GGATCAAACA TTTGGAAGAA 
GAGATGAGTT TAGAGAAAAT CAGTGTCACG TTGCCCTCAT CAGCAAGCTC CACCCTGCTT 
GGTGAAGACG CAGAGGTCGC AGCAGACTTT GACATGGAAG GTGACGTCGA TGACTACAGC 
GCTGAAGTAG AGGAGCTTCT TCCTCAGCAT CTGCAGCCGT CCTCAAGTTC TGGCCTGGGC 
ACTTCTCCAA GTTCTTCACC CCGGACCAGT CCCTGCCAGT CCCCCACAGT ACCAGAGTAC 
TCCGCCCCTT CTCTTCCCAT CAGACCTAGC CGAGCACCGT CAAGAACTCC AGGACCTCCA 
AGTTCGCAGG GCTCTCCAGT TGACACTCAG CCAGCGGCCC AGAAGGATTC TTCCCAGACT 
CTAGAGCCCA AGAGACCACC CCCTCCCCGC CCAGTCGCTC CTCCGGCACG TCCTGCCCCA 
CCACAGAGAC CACCTCCACC TTCAGGGGCT AGGAGTCCTG CGCCTGCTAG AAAAGAATTT 
GGAGGTGTTG GAGCCCCTCC CAGTCCCGGG GTTGCTAGGA GAGAGATAGA AGCACCCAAA 
AGCCCTGGAA CAGCACGGAA AGATAATATA GGGCGTAATC AGCCTTCCCC TCAAGCCGGA 
CTTGCAGGCC CAGGACCCGC TGGATACGGT GCGGCTAGAC CGACAATTCC AGCTCGTGCT 
GGAGTGATCA GCGCCCCTCA GAGCCAGGCT CGGGTATGTG CTGGAAGGCC GACTCCTGAC 
AGCCAAAGCA AGCCCTCGGA GACATTGAAA GGTCCTGCTG TCCTTCCAGA ACCACTGAAG 
CCTCAGGCTG CATTTCCTCA GCAGCCTTCT CTGCCCACAC CTGCTCAAAA GTTACAGGAC 
CCGCTTGTCC CCATAGCAGC ACCTACTATG CCTCCCTCTG GCCCCCAACC AAATTTGGAA 
ACCCCCCCAC AGCCCCCACC TCGGAGCAGG TCATCTCAAA GCTTGCCTTC AGACTCCTCA 
CCACAGCTGC AGGTAAAAAT AAATGGCATT TCTGGTGTCA AACAAGAGCC AACATTGAAG 
AGTGACCCAT TTGAAGATCT CTCACTTAGT GTGCTTGCTG TATCAAAGGC TCAGCCTTCT 
GTTCAGATTT CACCTGTTCT AACCCCAGAC CCAAAGATGT TGATTCAGTT GCCTTCTGCA 
TCGCAAAGTC AAGTTAACCC TTTGAGTTCT GTAAGTTGCA TGCCAACCAG GCCTCCAGGT 
CCAGAGGAAA GCAAGTCACA GGAGAGTATG GGCAGTTCTG CAAACCCGTT TCCCAGCCTG 
CCCTGCCGGA ACCCTTTTAC AGACAGGACT GCTGCCCCTG GAAACCCATT TAGAGTTCAG 
TCCCAAGAAT CAGAGGCAAC TTCTTGGCTC TCCAAAGAAG AGCCTGTTCC CAACAGTCCC 
TTCCCTCCTC TCATGCCTCT CAGTCATGAC ACGAGCAAGG CTTCAAGTTC GCTTGGTGGC 
TTTGAGGACA ATTTTGATCT GCAGAGCCAG TCTACAGTAA AAACAAGCAA CCCCAAAGGA 
TGGGTAACCT TTGACGAAGA CGACAACTTT CCTACAACAG GAAAGTCAAA GTCAGTTTGT 
CCAGACTTAG TGGGTAACGC GCCAGCTTCA TTTGATGATG ACTGGAGTAA AGGTGCGAGC 
GTTTCTTTCT GTGTGTTGCC AGCCAGAAGG CCACCACCAC CACCACCTCC TGTCCCTCTG 
CTACCACCTG GCACAACCTC ATCTGCAGGA CCTAGCACAA CCCTGCCATC TAAGGCACCT 
TCCACATTGG ACTTCACGGA AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246089.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244391.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OMu72080
Clone ID Related Accession (Same CDS sequence)	XM_011246089.3 , XM_011246089.2
Accession Version	XM_011246089.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA AGTTAAAAGA TGGTGCGCGA TCTGTCACCA GAACCATCCA GAATAACTTC 
TTCGACAGCT CCAAGCAGGA AGCCATTGAT GTCCTGCTCC TGGGAAATAC TCTCAACAGC 
GATTTAGCTG ACAAAGCCCG AGCGCTTCTA ACTACTGGAA GTTTGCGTGT TTCTGAACAG 
ACATTACAGT CAGCATCTTC CAAAGTCTTA AAGAACATGT GTGAGAACTT CTACAAATAC 
TCAAAGCCCA AGAAGATCCG AGTGTGTGTT GGCACCTGGA ATGTGAACGG CGGGAAGCAG 
TTCCGCAGCA TAGCGTTCAA GAACCAGACG CTCACAGACT GGCTTCTGGA CGCTCCCAAG 
TTAGCCGGCA TCCAGGAGTT TCAAGATAAG AGAAGTAAGC CAACTGATAT ATTTGCAATT 
GGCTTTGAAG AAATGGTGGA GCTGAATGCT GGGAACATTG TGAATGCAAG CACAACCAAC 
CAGAAGCTGT GGGCAGTGGA GCTGCAGAAG ACCATCTCCA GGGACAACAA GTATGTCCTG 
CTGGCCTCGG AGCAGCTGGT GGGTGTCTGC CTGTTTGTCT TTATCAGACC ACAGCACGCT 
CCTTTTATCA GGGATGTTGC AGTTGATACT GTGAAAACTG GCATGGGAGG TGCAACTGGA 
AACAAGGGCG CCGTTGCAAT TCGGATGCTC TTCCACACCA CCAGCCTTTG CTTCGTCTGC 
AGCCACTTTG CTGCAGGGCA GTCCCAAGTC AAAGAGAGAA ATGAAGATTT TGTAGAAATA 
GCACGAAAAC TGAGTTTCCC CATGGGACGA ATGCTCTTCT CCCATGACTA TGTATTTTGG 
TGTGGTGATT TCAACTATCG AATTGACCTT CCGAATGAAG AGGTTAAAGA GCTCATAAGA 
CAACAAAACT GGGATTCTCT GATCGCTGGG GATCAGCTCA TCAACCAGAA AAATGCAGGA 
CAGATCTTTA GAGGATTCTT AGAGGGAAAA GTAACGTTTG CTCCAACCTA TAAATATGAC 
TTGTTTTCTG AAGACTATGA CACCAGTGAG AAGTGTCGCA CCCCTGCTTG GACAGACCGT 
GTCCTCTGGA GAAGGAGGAA GTGGCCTTTT GACAGATCAG CTGAAGATTT AGATCTCCTG 
AATGCTAGTT TCCAAGATGA AAGTAAAATT CTTTATACAT GGACCCCTGG CACCTTGCTG 
CACTATGGAA GAGCCGAGCT GAAGACTTCT GACCATAGGC CCGTCGTTGC CTTGATTGAC 
ATAGATATAT TTGAAGTTGA AGCTGAAGAG AGACAAAAAA TTTATAAAGA AGTTATTGCC 
GTCCAGGGCC CACCAGATGG CACAGTGCTG GTCTCAATCA AAAGTTCTGC ACAAGAAAGT 
ACTTTTTTTG ATGATGCTTT GATTGATGAG CTTCTGCGGC AGTTTGCACA CTTTGGTGAA 
GTTATACTCA TAAGATTTGT AGAAGATAAA ATGTGGGTTA CATTTTTGGA GGGAAGCTCT 
GCCTTGAATG CTCTGAGCCT AAATGGGAAA GAGCTATTAA ATCGGACTAT AACAATTACT 
TTAAAAAGTC CAGACTGGAT CAAACATTTG GAAGAAGAGA TGAGTTTAGA GAAAATCAGT 
GTCACGTTGC CCTCATCAGC AAGCTCCACC CTGCTTGGTG AAGACGCAGA GGTCGCAGCA 
GACTTTGACA TGGAAGGTGA CGTCGATGAC TACAGCGCTG AAGTAGAGGA GCTTCTTCCT 
CAGCATCTGC AGCCGTCCTC AAGTTCTGGC CTGGGCACTT CTCCAAGTTC TTCACCCCGG 
ACCAGTCCCT GCCAGTCCCC CACAGTACCA GAGTACTCCG CCCCTTCTCT TCCCATCAGA 
CCTAGCCGAG CACCGTCAAG AACTCCAGGA CCTCCAAGTT CGCAGGGCTC TCCAGTTGAC 
ACTCAGCCAG CGGCCCAGAA GGATTCTTCC CAGACTCTAG AGCCCAAGAG ACCACCCCCT 
CCCCGCCCAG TCGCTCCTCC GGCACGTCCT GCCCCACCAC AGAGACCACC TCCACCTTCA 
GGGGCTAGGA GTCCTGCGCC TGCTAGAAAA GAATTTGGAG GTGTTGGAGC CCCTCCCAGT 
CCCGGGGTTG CTAGGAGAGA GATAGAAGCA CCCAAAAGCC CTGGAACAGC ACGGAAAGAT 
AATATAGGGC GTAATCAGCC TTCCCCTCAA GCCGGACTTG CAGGCCCAGG ACCCGCTGGA 
TACGGTGCGG CTAGACCGAC AATTCCAGCT CGTGCTGGAG TGATCAGCGC CCCTCAGAGC 
CAGGCTCGGG TATGTGCTGG AAGGCCGACT CCTGACAGCC AAAGCAAGCC CTCGGAGACA 
TTGAAAGGTC CTGCTGTCCT TCCAGAACCA CTGAAGCCTC AGGCTGCATT TCCTCAGCAG 
CCTTCTCTGC CCACACCTGC TCAAAAGTTA CAGGACCCGC TTGTCCCCAT AGCAGCACCT 
ACTATGCCTC CCTCTGGCCC CCAACCAAAT TTGGAAACCC CCCCACAGCC CCCACCTCGG 
AGCAGGTCAT CTCAAAGCTT GCCTTCAGAC TCCTCACCAC AGCTGCAGGT AAAAATAAAT 
GGCATTTCTG GTGTCAAACA AGAGCCAACA TTGAAGAGTG ACCCATTTGA AGATCTCTCA 
CTTAGTGTGC TTGCTGTATC AAAGGCTCAG CCTTCTGTTC AGATTTCACC TGTTCTAACC 
CCAGACCCAA AGATGTTGAT TCAGTTGCCT TCTGCATCGC AAAGTCAAGT TAACCCTTTG 
AGTTCTGTAA GTTGCATGCC AACCAGGCCT CCAGGTCCAG AGGAAAGCAA GTCACAGGAG 
AGTATGGGCA GTTCTGCAAA CCCGTTTCCC AGCCTGCCCT GCCGGAACCC TTTTACAGAC 
AGGACTGCTG CCCCTGGAAA CCCATTTAGA GTTCAGTCCC AAGAATCAGA GGCAACTTCT 
TGGCTCTCCA AAGAAGAGCC TGTTCCCAAC AGTCCCTTCC CTCCTCTCAT GCCTCTCAGT 
CATGACACGA GCAAGGCTTC AAGTTCGCTT GGTGGCTTTG AGGACAATTT TGATCTGCAG 
AGCCAGTCTA CAGTAAAAAC AAGCAACCCC AAAGGATGGG TAACCTTTGA CGAAGACGAC 
AACTTTCCTA CAACAGGAAA GTCAAAGTCA GTTTGTCCAG ACTTAGTGGG TAACGCGCCA 
GCTTCATTTG ATGATGACTG GAGTAAAGGT GCGAGCGTTT CTTTCTGTGT GTTGCCAGCC 
AGAAGGCCAC CACCACCACC ACCTCCTGTC CCTCTGCTAC CACCTGGCAC AACCTCATCT 
GCAGGACCTA GCACAACCCT GCCATCTAAG GCACCTTCCA CATTGGACTT CACGGAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244391.1
CDS	518..3880
Translation	MAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246089.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246089.2

ORF Insert Sequence:

ATGGCTGGAA AGTTAAAAGA TGGTGCGCGA TCTGTCACCA GAACCATCCA GAATAACTTC 
TTCGACAGCT CCAAGCAGGA AGCCATTGAT GTCCTGCTCC TGGGAAATAC TCTCAACAGC 
GATTTAGCTG ACAAAGCCCG AGCGCTTCTA ACTACTGGAA GTTTGCGTGT TTCTGAACAG 
ACATTACAGT CAGCATCTTC CAAAGTCTTA AAGAACATGT GTGAGAACTT CTACAAATAC 
TCAAAGCCCA AGAAGATCCG AGTGTGTGTT GGCACCTGGA ATGTGAACGG CGGGAAGCAG 
TTCCGCAGCA TAGCGTTCAA GAACCAGACG CTCACAGACT GGCTTCTGGA CGCTCCCAAG 
TTAGCCGGCA TCCAGGAGTT TCAAGATAAG AGAAGTAAGC CAACTGATAT ATTTGCAATT 
GGCTTTGAAG AAATGGTGGA GCTGAATGCT GGGAACATTG TGAATGCAAG CACAACCAAC 
CAGAAGCTGT GGGCAGTGGA GCTGCAGAAG ACCATCTCCA GGGACAACAA GTATGTCCTG 
CTGGCCTCGG AGCAGCTGGT GGGTGTCTGC CTGTTTGTCT TTATCAGACC ACAGCACGCT 
CCTTTTATCA GGGATGTTGC AGTTGATACT GTGAAAACTG GCATGGGAGG TGCAACTGGA 
AACAAGGGCG CCGTTGCAAT TCGGATGCTC TTCCACACCA CCAGCCTTTG CTTCGTCTGC 
AGCCACTTTG CTGCAGGGCA GTCCCAAGTC AAAGAGAGAA ATGAAGATTT TGTAGAAATA 
GCACGAAAAC TGAGTTTCCC CATGGGACGA ATGCTCTTCT CCCATGACTA TGTATTTTGG 
TGTGGTGATT TCAACTATCG AATTGACCTT CCGAATGAAG AGGTTAAAGA GCTCATAAGA 
CAACAAAACT GGGATTCTCT GATCGCTGGG GATCAGCTCA TCAACCAGAA AAATGCAGGA 
CAGATCTTTA GAGGATTCTT AGAGGGAAAA GTAACGTTTG CTCCAACCTA TAAATATGAC 
TTGTTTTCTG AAGACTATGA CACCAGTGAG AAGTGTCGCA CCCCTGCTTG GACAGACCGT 
GTCCTCTGGA GAAGGAGGAA GTGGCCTTTT GACAGATCAG CTGAAGATTT AGATCTCCTG 
AATGCTAGTT TCCAAGATGA AAGTAAAATT CTTTATACAT GGACCCCTGG CACCTTGCTG 
CACTATGGAA GAGCCGAGCT GAAGACTTCT GACCATAGGC CCGTCGTTGC CTTGATTGAC 
ATAGATATAT TTGAAGTTGA AGCTGAAGAG AGACAAAAAA TTTATAAAGA AGTTATTGCC 
GTCCAGGGCC CACCAGATGG CACAGTGCTG GTCTCAATCA AAAGTTCTGC ACAAGAAAGT 
ACTTTTTTTG ATGATGCTTT GATTGATGAG CTTCTGCGGC AGTTTGCACA CTTTGGTGAA 
GTTATACTCA TAAGATTTGT AGAAGATAAA ATGTGGGTTA CATTTTTGGA GGGAAGCTCT 
GCCTTGAATG CTCTGAGCCT AAATGGGAAA GAGCTATTAA ATCGGACTAT AACAATTACT 
TTAAAAAGTC CAGACTGGAT CAAACATTTG GAAGAAGAGA TGAGTTTAGA GAAAATCAGT 
GTCACGTTGC CCTCATCAGC AAGCTCCACC CTGCTTGGTG AAGACGCAGA GGTCGCAGCA 
GACTTTGACA TGGAAGGTGA CGTCGATGAC TACAGCGCTG AAGTAGAGGA GCTTCTTCCT 
CAGCATCTGC AGCCGTCCTC AAGTTCTGGC CTGGGCACTT CTCCAAGTTC TTCACCCCGG 
ACCAGTCCCT GCCAGTCCCC CACAGTACCA GAGTACTCCG CCCCTTCTCT TCCCATCAGA 
CCTAGCCGAG CACCGTCAAG AACTCCAGGA CCTCCAAGTT CGCAGGGCTC TCCAGTTGAC 
ACTCAGCCAG CGGCCCAGAA GGATTCTTCC CAGACTCTAG AGCCCAAGAG ACCACCCCCT 
CCCCGCCCAG TCGCTCCTCC GGCACGTCCT GCCCCACCAC AGAGACCACC TCCACCTTCA 
GGGGCTAGGA GTCCTGCGCC TGCTAGAAAA GAATTTGGAG GTGTTGGAGC CCCTCCCAGT 
CCCGGGGTTG CTAGGAGAGA GATAGAAGCA CCCAAAAGCC CTGGAACAGC ACGGAAAGAT 
AATATAGGGC GTAATCAGCC TTCCCCTCAA GCCGGACTTG CAGGCCCAGG ACCCGCTGGA 
TACGGTGCGG CTAGACCGAC AATTCCAGCT CGTGCTGGAG TGATCAGCGC CCCTCAGAGC 
CAGGCTCGGG TATGTGCTGG AAGGCCGACT CCTGACAGCC AAAGCAAGCC CTCGGAGACA 
TTGAAAGGTC CTGCTGTCCT TCCAGAACCA CTGAAGCCTC AGGCTGCATT TCCTCAGCAG 
CCTTCTCTGC CCACACCTGC TCAAAAGTTA CAGGACCCGC TTGTCCCCAT AGCAGCACCT 
ACTATGCCTC CCTCTGGCCC CCAACCAAAT TTGGAAACCC CCCCACAGCC CCCACCTCGG 
AGCAGGTCAT CTCAAAGCTT GCCTTCAGAC TCCTCACCAC AGCTGCAGGT AAAAATAAAT 
GGCATTTCTG GTGTCAAACA AGAGCCAACA TTGAAGAGTG ACCCATTTGA AGATCTCTCA 
CTTAGTGTGC TTGCTGTATC AAAGGCTCAG CCTTCTGTTC AGATTTCACC TGTTCTAACC 
CCAGACCCAA AGATGTTGAT TCAGTTGCCT TCTGCATCGC AAAGTCAAGT TAACCCTTTG 
AGTTCTGTAA GTTGCATGCC AACCAGGCCT CCAGGTCCAG AGGAAAGCAA GTCACAGGAG 
AGTATGGGCA GTTCTGCAAA CCCGTTTCCC AGCCTGCCCT GCCGGAACCC TTTTACAGAC 
AGGACTGCTG CCCCTGGAAA CCCATTTAGA GTTCAGTCCC AAGAATCAGA GGCAACTTCT 
TGGCTCTCCA AAGAAGAGCC TGTTCCCAAC AGTCCCTTCC CTCCTCTCAT GCCTCTCAGT 
CATGACACGA GCAAGGCTTC AAGTTCGCTT GGTGGCTTTG AGGACAATTT TGATCTGCAG 
AGCCAGTCTA CAGTAAAAAC AAGCAACCCC AAAGGATGGG TAACCTTTGA CGAAGACGAC 
AACTTTCCTA CAACAGGAAA GTCAAAGTCA GTTTGTCCAG ACTTAGTGGG TAACGCGCCA 
GCTTCATTTG ATGATGACTG GAGTAAAGGT GCGAGCGTTT CTTTCTGTGT GTTGCCAGCC 
AGAAGGCCAC CACCACCACC ACCTCCTGTC CCTCTGCTAC CACCTGGCAC AACCTCATCT 
GCAGGACCTA GCACAACCCT GCCATCTAAG GCACCTTCCA CATTGGACTT CACGGAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_006522837.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522900.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X6, mRNA.

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CloneID	OMu52615
Clone ID Related Accession (Same CDS sequence)	XM_006522837.3 , XM_006522837.4
Accession Version	XM_006522837.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522837.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGCACCCAA AAGCCCTGGA ACAGCACGGA AAGATAATAT AGGGCGTAAT 
CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC CCAGGACCCG CTGGATACGG TGCGGCTAGA 
CCGACAATTC CAGCTCGTGC TGGAGTGATC AGCGCCCCTC AGAGCCAGGC TCGGGTATGT 
GCTGGAAGGC CGACTCCTGA CAGCCAAAGC AAGCCCTCGG AGACATTGAA AGGTCCTGCT 
GTCCTTCCAG AACCACTGAA GCCTCAGGCT GCATTTCCTC AGCAGCCTTC TCTGCCCACA 
CCTGCTCAAA AGTTACAGGA CCCGCTTGTC CCCATAGCAG CACCTACTAT GCCTCCCTCT 
GGCCCCCAAC CAAATTTGGA AACCCCCCCA CAGCCCCCAC CTCGGAGCAG GTCATCTCAA 
AGCTTGCCTT CAGACTCCTC ACCACAGCTG CAGGTAAAAA TAAATGGCAT TTCTGGTGTC 
AAACAAGAGC CAACATTGAA GAGTGACCCA TTTGAAGATC TCTCACTTAG TGTGCTTGCT 
GTATCAAAGG CTCAGCCTTC TGTTCAGATT TCACCTGTTC TAACCCCAGA CCCAAAGATG 
TTGATTCAGT TGCCTTCTGC ATCGCAAAGT CAAGTTAACC CTTTGAGTTC TGTAAGTTGC 
ATGCCAACCA GGCCTCCAGG TCCAGAGGAA AGCAAGTCAC AGGAGAGTAT GGGCAGTTCT 
GCAAACCCGT TTCCCAGCCT GCCCTGCCGG AACCCTTTTA CAGACAGGAC TGCTGCCCCT 
GGAAACCCAT TTAGAGTTCA GTCCCAAGAA TCAGAGGCAA CTTCTTGGCT CTCCAAAGAA 
GAGCCTGTTC CCAACAGTCC CTTCCCTCCT CTCATGCCTC TCAGTCATGA CACGAGCAAG 
GCTTCAAGTT CGCTTGGTGG CTTTGAGGAC AATTTTGATC TGCAGAGCCA GTCTACAGTA 
AAAACAAGCA ACCCCAAAGG ATGGGTAACC TTTGACGAAG ACGACAACTT TCCTACAACA 
GGAAAGTCAA AGTCAGTTTG TCCAGACTTA GTGGGTAACG CGCCAGCTTC ATTTGATGAT 
GACTGGAGTA AAGGTGCGAG CGTTTCTTTC TGTGTGTTGC CAGCCAGAAG GCCACCACCA 
CCACCACCTC CTGTCCCTCT GCTACCACCT GGCACAACCT CATCTGCAGG ACCTAGCACA 
ACCCTGCCAT CTAAGGCACC TTCCACATTG GACTTCACGG AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522900.1
CDS	2226..7313
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_006522837.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522837.4

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGCACCCAA AAGCCCTGGA ACAGCACGGA AAGATAATAT AGGGCGTAAT 
CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC CCAGGACCCG CTGGATACGG TGCGGCTAGA 
CCGACAATTC CAGCTCGTGC TGGAGTGATC AGCGCCCCTC AGAGCCAGGC TCGGGTATGT 
GCTGGAAGGC CGACTCCTGA CAGCCAAAGC AAGCCCTCGG AGACATTGAA AGGTCCTGCT 
GTCCTTCCAG AACCACTGAA GCCTCAGGCT GCATTTCCTC AGCAGCCTTC TCTGCCCACA 
CCTGCTCAAA AGTTACAGGA CCCGCTTGTC CCCATAGCAG CACCTACTAT GCCTCCCTCT 
GGCCCCCAAC CAAATTTGGA AACCCCCCCA CAGCCCCCAC CTCGGAGCAG GTCATCTCAA 
AGCTTGCCTT CAGACTCCTC ACCACAGCTG CAGGTAAAAA TAAATGGCAT TTCTGGTGTC 
AAACAAGAGC CAACATTGAA GAGTGACCCA TTTGAAGATC TCTCACTTAG TGTGCTTGCT 
GTATCAAAGG CTCAGCCTTC TGTTCAGATT TCACCTGTTC TAACCCCAGA CCCAAAGATG 
TTGATTCAGT TGCCTTCTGC ATCGCAAAGT CAAGTTAACC CTTTGAGTTC TGTAAGTTGC 
ATGCCAACCA GGCCTCCAGG TCCAGAGGAA AGCAAGTCAC AGGAGAGTAT GGGCAGTTCT 
GCAAACCCGT TTCCCAGCCT GCCCTGCCGG AACCCTTTTA CAGACAGGAC TGCTGCCCCT 
GGAAACCCAT TTAGAGTTCA GTCCCAAGAA TCAGAGGCAA CTTCTTGGCT CTCCAAAGAA 
GAGCCTGTTC CCAACAGTCC CTTCCCTCCT CTCATGCCTC TCAGTCATGA CACGAGCAAG 
GCTTCAAGTT CGCTTGGTGG CTTTGAGGAC AATTTTGATC TGCAGAGCCA GTCTACAGTA 
AAAACAAGCA ACCCCAAAGG ATGGGTAACC TTTGACGAAG ACGACAACTT TCCTACAACA 
GGAAAGTCAA AGTCAGTTTG TCCAGACTTA GTGGGTAACG CGCCAGCTTC ATTTGATGAT 
GACTGGAGTA AAGGTGCGAG CGTTTCTTTC TGTGTGTTGC CAGCCAGAAG GCCACCACCA 
CCACCACCTC CTGTCCCTCT GCTACCACCT GGCACAACCT CATCTGCAGG ACCTAGCACA 
ACCCTGCCAT CTAAGGCACC TTCCACATTG GACTTCACGG AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246075.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244377.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X1, mRNA.

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CloneID	OMu72067
Clone ID Related Accession (Same CDS sequence)	XM_011246075.2 , XM_011246075.3
Accession Version	XM_011246075.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246075.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG GTAAAAATAA ATGGCATTTC TGGTGTCAAA 
CAAGAGCCAA CATTGAAGAG TGACCCATTT GAAGATCTCT CACTTAGTGT GCTTGCTGTA 
TCAAAGGCTC AGCCTTCTGT TCAGATTTCA CCTGTTCTAA CCCCAGACCC AAAGATGTTG 
ATTCAGTTGC CTTCTGCATC GCAAAGTCAA GTTAACCCTT TGAGTTCTGT AAGTTGCATG 
CCAACCAGGC CTCCAGGTCC AGAGGAAAGC AAGTCACAGG AGAGTATGGG CAGTTCTGCA 
AACCCGTTTC CCAGCCTGCC CTGCCGGAAC CCTTTTACAG ACAGGACTGC TGCCCCTGGA 
AACCCATTTA GAGTTCAGTC CCAAGAATCA GAGGCAACTT CTTGGCTCTC CAAAGAAGAG 
CCTGTTCCCA ACAGTCCCTT CCCTCCTCTC ATGCCTCTCA GTCATGACAC GAGCAAGGCT 
TCAAGTTCGC TTGGTGGCTT TGAGGACAAT TTTGATCTGC AGAGCCAGTC TACAGTAAAA 
ACAAGCAACC CCAAAGGATG GGTAACCTTT GACGAAGACG ACAACTTTCC TACAACAGGA 
AAGTCAAAGT CAGTTTGTCC AGACTTAGTG GGTAACGCGC CAGCTTCATT TGATGATGAC 
TGGAGTAAAG GTGCGAGCGT TTCTTTCTGT GTGTTGCCAG CCAGAAGGCC ACCACCACCA 
CCACCTCCTG TCCCTCTGCT ACCACCTGGC ACAACCTCAT CTGCAGGACC TAGCACAACC 
CTGCCATCTA AGGCACCTTC CACATTGGAC TTCACGGAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244377.1
CDS	2226..7370
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246075.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246075.3

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG GTAAAAATAA ATGGCATTTC TGGTGTCAAA 
CAAGAGCCAA CATTGAAGAG TGACCCATTT GAAGATCTCT CACTTAGTGT GCTTGCTGTA 
TCAAAGGCTC AGCCTTCTGT TCAGATTTCA CCTGTTCTAA CCCCAGACCC AAAGATGTTG 
ATTCAGTTGC CTTCTGCATC GCAAAGTCAA GTTAACCCTT TGAGTTCTGT AAGTTGCATG 
CCAACCAGGC CTCCAGGTCC AGAGGAAAGC AAGTCACAGG AGAGTATGGG CAGTTCTGCA 
AACCCGTTTC CCAGCCTGCC CTGCCGGAAC CCTTTTACAG ACAGGACTGC TGCCCCTGGA 
AACCCATTTA GAGTTCAGTC CCAAGAATCA GAGGCAACTT CTTGGCTCTC CAAAGAAGAG 
CCTGTTCCCA ACAGTCCCTT CCCTCCTCTC ATGCCTCTCA GTCATGACAC GAGCAAGGCT 
TCAAGTTCGC TTGGTGGCTT TGAGGACAAT TTTGATCTGC AGAGCCAGTC TACAGTAAAA 
ACAAGCAACC CCAAAGGATG GGTAACCTTT GACGAAGACG ACAACTTTCC TACAACAGGA 
AAGTCAAAGT CAGTTTGTCC AGACTTAGTG GGTAACGCGC CAGCTTCATT TGATGATGAC 
TGGAGTAAAG GTGCGAGCGT TTCTTTCTGT GTGTTGCCAG CCAGAAGGCC ACCACCACCA 
CCACCTCCTG TCCCTCTGCT ACCACCTGGC ACAACCTCAT CTGCAGGACC TAGCACAACC 
CTGCCATCTA AGGCACCTTC CACATTGGAC TTCACGGAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246076.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244378.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X2, mRNA.

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CloneID	OMu72068
Clone ID Related Accession (Same CDS sequence)	XM_011246076.2 , XM_011246076.3
Accession Version	XM_011246076.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GCATCTTCCA AAGTCTTAAA GAACATGTGT 
GAGAACTTCT ACAAATACTC AAAGCCCAAG AAGATCCGAG TGTGTGTTGG CACCTGGAAT 
GTGAACGGCG GGAAGCAGTT CCGCAGCATA GCGTTCAAGA ACCAGACGCT CACAGACTGG 
CTTCTGGACG CTCCCAAGTT AGCCGGCATC CAGGAGTTTC AAGATAAGAG AAGTAAGCCA 
ACTGATATAT TTGCAATTGG CTTTGAAGAA ATGGTGGAGC TGAATGCTGG GAACATTGTG 
AATGCAAGCA CAACCAACCA GAAGCTGTGG GCAGTGGAGC TGCAGAAGAC CATCTCCAGG 
GACAACAAGT ATGTCCTGCT GGCCTCGGAG CAGCTGGTGG GTGTCTGCCT GTTTGTCTTT 
ATCAGACCAC AGCACGCTCC TTTTATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGC 
ATGGGAGGTG CAACTGGAAA CAAGGGCGCC GTTGCAATTC GGATGCTCTT CCACACCACC 
AGCCTTTGCT TCGTCTGCAG CCACTTTGCT GCAGGGCAGT CCCAAGTCAA AGAGAGAAAT 
GAAGATTTTG TAGAAATAGC ACGAAAACTG AGTTTCCCCA TGGGACGAAT GCTCTTCTCC 
CATGACTATG TATTTTGGTG TGGTGATTTC AACTATCGAA TTGACCTTCC GAATGAAGAG 
GTTAAAGAGC TCATAAGACA ACAAAACTGG GATTCTCTGA TCGCTGGGGA TCAGCTCATC 
AACCAGAAAA ATGCAGGACA GATCTTTAGA GGATTCTTAG AGGGAAAAGT AACGTTTGCT 
CCAACCTATA AATATGACTT GTTTTCTGAA GACTATGACA CCAGTGAGAA GTGTCGCACC 
CCTGCTTGGA CAGACCGTGT CCTCTGGAGA AGGAGGAAGT GGCCTTTTGA CAGATCAGCT 
GAAGATTTAG ATCTCCTGAA TGCTAGTTTC CAAGATGAAA GTAAAATTCT TTATACATGG 
ACCCCTGGCA CCTTGCTGCA CTATGGAAGA GCCGAGCTGA AGACTTCTGA CCATAGGCCC 
GTCGTTGCCT TGATTGACAT AGATATATTT GAAGTTGAAG CTGAAGAGAG ACAAAAAATT 
TATAAAGAAG TTATTGCCGT CCAGGGCCCA CCAGATGGCA CAGTGCTGGT CTCAATCAAA 
AGTTCTGCAC AAGAAAGTAC TTTTTTTGAT GATGCTTTGA TTGATGAGCT TCTGCGGCAG 
TTTGCACACT TTGGTGAAGT TATACTCATA AGATTTGTAG AAGATAAAAT GTGGGTTACA 
TTTTTGGAGG GAAGCTCTGC CTTGAATGCT CTGAGCCTAA ATGGGAAAGA GCTATTAAAT 
CGGACTATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AACATTTGGA AGAAGAGATG 
AGTTTAGAGA AAATCAGTGT CACGTTGCCC TCATCAGCAA GCTCCACCCT GCTTGGTGAA 
GACGCAGAGG TCGCAGCAGA CTTTGACATG GAAGGTGACG TCGATGACTA CAGCGCTGAA 
GTAGAGGAGC TTCTTCCTCA GCATCTGCAG CCGTCCTCAA GTTCTGGCCT GGGCACTTCT 
CCAAGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCCCCCA CAGTACCAGA GTACTCCGCC 
CCTTCTCTTC CCATCAGACC TAGCCGAGCA CCGTCAAGAA CTCCAGGACC TCCAAGTTCG 
CAGGGCTCTC CAGTTGACAC TCAGCCAGCG GCCCAGAAGG ATTCTTCCCA GACTCTAGAG 
CCCAAGAGAC CACCCCCTCC CCGCCCAGTC GCTCCTCCGG CACGTCCTGC CCCACCACAG 
AGACCACCTC CACCTTCAGG GGCTAGGAGT CCTGCGCCTG CTAGAAAAGA ATTTGGAGGT 
GTTGGAGCCC CTCCCAGTCC CGGGGTTGCT AGGAGAGAGA TAGAAGCACC CAAAAGCCCT 
GGAACAGCAC GGAAAGATAA TATAGGGCGT AATCAGCCTT CCCCTCAAGC CGGACTTGCA 
GGCCCAGGAC CCGCTGGATA CGGTGCGGCT AGACCGACAA TTCCAGCTCG TGCTGGAGTG 
ATCAGCGCCC CTCAGAGCCA GGCTCGGGTA TGTGCTGGAA GGCCGACTCC TGACAGCCAA 
AGCAAGCCCT CGGAGACATT GAAAGGTCCT GCTGTCCTTC CAGAACCACT GAAGCCTCAG 
GCTGCATTTC CTCAGCAGCC TTCTCTGCCC ACACCTGCTC AAAAGTTACA GGACCCGCTT 
GTCCCCATAG CAGCACCTAC TATGCCTCCC TCTGGCCCCC AACCAAATTT GGAAACCCCC 
CCACAGCCCC CACCTCGGAG CAGGTCATCT CAAAGCTTGC CTTCAGACTC CTCACCACAG 
CTGCAGGTAA AAATAAATGG CATTTCTGGT GTCAAACAAG AGCCAACATT GAAGAGTGAC 
CCATTTGAAG ATCTCTCACT TAGTGTGCTT GCTGTATCAA AGGCTCAGCC TTCTGTTCAG 
ATTTCACCTG TTCTAACCCC AGACCCAAAG ATGTTGATTC AGTTGCCTTC TGCATCGCAA 
AGTCAAGTTA ACCCTTTGAG TTCTGTAAGT TGCATGCCAA CCAGGCCTCC AGGTCCAGAG 
GAAAGCAAGT CACAGGAGAG TATGGGCAGT TCTGCAAACC CGTTTCCCAG CCTGCCCTGC 
CGGAACCCTT TTACAGACAG GACTGCTGCC CCTGGAAACC CATTTAGAGT TCAGTCCCAA 
GAATCAGAGG CAACTTCTTG GCTCTCCAAA GAAGAGCCTG TTCCCAACAG TCCCTTCCCT 
CCTCTCATGC CTCTCAGTCA TGACACGAGC AAGGCTTCAA GTTCGCTTGG TGGCTTTGAG 
GACAATTTTG ATCTGCAGAG CCAGTCTACA GTAAAAACAA GCAACCCCAA AGGATGGGTA 
ACCTTTGACG AAGACGACAA CTTTCCTACA ACAGGAAAGT CAAAGTCAGT TTGTCCAGAC 
TTAGTGGGTA ACGCGCCAGC TTCATTTGAT GATGACTGGA GTAAAGGTGC GAGCGTTTCT 
TTCTGTGTGT TGCCAGCCAG AAGGCCACCA CCACCACCAC CTCCTGTCCC TCTGCTACCA 
CCTGGCACAA CCTCATCTGC AGGACCTAGC ACAACCCTGC CATCTAAGGC ACCTTCCACA 
TTGGACTTCA CGGAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244378.1
CDS	2226..7346
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246076.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246076.3

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GCATCTTCCA AAGTCTTAAA GAACATGTGT 
GAGAACTTCT ACAAATACTC AAAGCCCAAG AAGATCCGAG TGTGTGTTGG CACCTGGAAT 
GTGAACGGCG GGAAGCAGTT CCGCAGCATA GCGTTCAAGA ACCAGACGCT CACAGACTGG 
CTTCTGGACG CTCCCAAGTT AGCCGGCATC CAGGAGTTTC AAGATAAGAG AAGTAAGCCA 
ACTGATATAT TTGCAATTGG CTTTGAAGAA ATGGTGGAGC TGAATGCTGG GAACATTGTG 
AATGCAAGCA CAACCAACCA GAAGCTGTGG GCAGTGGAGC TGCAGAAGAC CATCTCCAGG 
GACAACAAGT ATGTCCTGCT GGCCTCGGAG CAGCTGGTGG GTGTCTGCCT GTTTGTCTTT 
ATCAGACCAC AGCACGCTCC TTTTATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGC 
ATGGGAGGTG CAACTGGAAA CAAGGGCGCC GTTGCAATTC GGATGCTCTT CCACACCACC 
AGCCTTTGCT TCGTCTGCAG CCACTTTGCT GCAGGGCAGT CCCAAGTCAA AGAGAGAAAT 
GAAGATTTTG TAGAAATAGC ACGAAAACTG AGTTTCCCCA TGGGACGAAT GCTCTTCTCC 
CATGACTATG TATTTTGGTG TGGTGATTTC AACTATCGAA TTGACCTTCC GAATGAAGAG 
GTTAAAGAGC TCATAAGACA ACAAAACTGG GATTCTCTGA TCGCTGGGGA TCAGCTCATC 
AACCAGAAAA ATGCAGGACA GATCTTTAGA GGATTCTTAG AGGGAAAAGT AACGTTTGCT 
CCAACCTATA AATATGACTT GTTTTCTGAA GACTATGACA CCAGTGAGAA GTGTCGCACC 
CCTGCTTGGA CAGACCGTGT CCTCTGGAGA AGGAGGAAGT GGCCTTTTGA CAGATCAGCT 
GAAGATTTAG ATCTCCTGAA TGCTAGTTTC CAAGATGAAA GTAAAATTCT TTATACATGG 
ACCCCTGGCA CCTTGCTGCA CTATGGAAGA GCCGAGCTGA AGACTTCTGA CCATAGGCCC 
GTCGTTGCCT TGATTGACAT AGATATATTT GAAGTTGAAG CTGAAGAGAG ACAAAAAATT 
TATAAAGAAG TTATTGCCGT CCAGGGCCCA CCAGATGGCA CAGTGCTGGT CTCAATCAAA 
AGTTCTGCAC AAGAAAGTAC TTTTTTTGAT GATGCTTTGA TTGATGAGCT TCTGCGGCAG 
TTTGCACACT TTGGTGAAGT TATACTCATA AGATTTGTAG AAGATAAAAT GTGGGTTACA 
TTTTTGGAGG GAAGCTCTGC CTTGAATGCT CTGAGCCTAA ATGGGAAAGA GCTATTAAAT 
CGGACTATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AACATTTGGA AGAAGAGATG 
AGTTTAGAGA AAATCAGTGT CACGTTGCCC TCATCAGCAA GCTCCACCCT GCTTGGTGAA 
GACGCAGAGG TCGCAGCAGA CTTTGACATG GAAGGTGACG TCGATGACTA CAGCGCTGAA 
GTAGAGGAGC TTCTTCCTCA GCATCTGCAG CCGTCCTCAA GTTCTGGCCT GGGCACTTCT 
CCAAGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCCCCCA CAGTACCAGA GTACTCCGCC 
CCTTCTCTTC CCATCAGACC TAGCCGAGCA CCGTCAAGAA CTCCAGGACC TCCAAGTTCG 
CAGGGCTCTC CAGTTGACAC TCAGCCAGCG GCCCAGAAGG ATTCTTCCCA GACTCTAGAG 
CCCAAGAGAC CACCCCCTCC CCGCCCAGTC GCTCCTCCGG CACGTCCTGC CCCACCACAG 
AGACCACCTC CACCTTCAGG GGCTAGGAGT CCTGCGCCTG CTAGAAAAGA ATTTGGAGGT 
GTTGGAGCCC CTCCCAGTCC CGGGGTTGCT AGGAGAGAGA TAGAAGCACC CAAAAGCCCT 
GGAACAGCAC GGAAAGATAA TATAGGGCGT AATCAGCCTT CCCCTCAAGC CGGACTTGCA 
GGCCCAGGAC CCGCTGGATA CGGTGCGGCT AGACCGACAA TTCCAGCTCG TGCTGGAGTG 
ATCAGCGCCC CTCAGAGCCA GGCTCGGGTA TGTGCTGGAA GGCCGACTCC TGACAGCCAA 
AGCAAGCCCT CGGAGACATT GAAAGGTCCT GCTGTCCTTC CAGAACCACT GAAGCCTCAG 
GCTGCATTTC CTCAGCAGCC TTCTCTGCCC ACACCTGCTC AAAAGTTACA GGACCCGCTT 
GTCCCCATAG CAGCACCTAC TATGCCTCCC TCTGGCCCCC AACCAAATTT GGAAACCCCC 
CCACAGCCCC CACCTCGGAG CAGGTCATCT CAAAGCTTGC CTTCAGACTC CTCACCACAG 
CTGCAGGTAA AAATAAATGG CATTTCTGGT GTCAAACAAG AGCCAACATT GAAGAGTGAC 
CCATTTGAAG ATCTCTCACT TAGTGTGCTT GCTGTATCAA AGGCTCAGCC TTCTGTTCAG 
ATTTCACCTG TTCTAACCCC AGACCCAAAG ATGTTGATTC AGTTGCCTTC TGCATCGCAA 
AGTCAAGTTA ACCCTTTGAG TTCTGTAAGT TGCATGCCAA CCAGGCCTCC AGGTCCAGAG 
GAAAGCAAGT CACAGGAGAG TATGGGCAGT TCTGCAAACC CGTTTCCCAG CCTGCCCTGC 
CGGAACCCTT TTACAGACAG GACTGCTGCC CCTGGAAACC CATTTAGAGT TCAGTCCCAA 
GAATCAGAGG CAACTTCTTG GCTCTCCAAA GAAGAGCCTG TTCCCAACAG TCCCTTCCCT 
CCTCTCATGC CTCTCAGTCA TGACACGAGC AAGGCTTCAA GTTCGCTTGG TGGCTTTGAG 
GACAATTTTG ATCTGCAGAG CCAGTCTACA GTAAAAACAA GCAACCCCAA AGGATGGGTA 
ACCTTTGACG AAGACGACAA CTTTCCTACA ACAGGAAAGT CAAAGTCAGT TTGTCCAGAC 
TTAGTGGGTA ACGCGCCAGC TTCATTTGAT GATGACTGGA GTAAAGGTGC GAGCGTTTCT 
TTCTGTGTGT TGCCAGCCAG AAGGCCACCA CCACCACCAC CTCCTGTCCC TCTGCTACCA 
CCTGGCACAA CCTCATCTGC AGGACCTAGC ACAACCCTGC CATCTAAGGC ACCTTCCACA 
TTGGACTTCA CGGAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246077.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244379.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu72069
Clone ID Related Accession (Same CDS sequence)	XM_011246077.2 , XM_011246077.3
Accession Version	XM_011246077.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246077.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGTGTTGG AGCCCCTCCC 
AGTCCCGGGG TTGCTAGGAG AGAGATAGAA GCACCCAAAA GCCCTGGAAC AGCACGGAAA 
GATAATATAG GGCGTAATCA GCCTTCCCCT CAAGCCGGAC TTGCAGGCCC AGGACCCGCT 
GGATACGGTG CGGCTAGACC GACAATTCCA GCTCGTGCTG GAGTGATCAG CGCCCCTCAG 
AGCCAGGCTC GGGTATGTGC TGGAAGGCCG ACTCCTGACA GCCAAAGCAA GCCCTCGGAG 
ACATTGAAAG GTCCTGCTGT CCTTCCAGAA CCACTGAAGC CTCAGGCTGC ATTTCCTCAG 
CAGCCTTCTC TGCCCACACC TGCTCAAAAG TTACAGGACC CGCTTGTCCC CATAGCAGCA 
CCTACTATGC CTCCCTCTGG CCCCCAACCA AATTTGGAAA CCCCCCCACA GCCCCCACCT 
CGGAGCAGGT CATCTCAAAG CTTGCCTTCA GACTCCTCAC CACAGCTGCA GGTAAAAATA 
AATGGCATTT CTGGTGTCAA ACAAGAGCCA ACATTGAAGA GTGACCCATT TGAAGATCTC 
TCACTTAGTG TGCTTGCTGT ATCAAAGGCT CAGCCTTCTG TTCAGATTTC ACCTGTTCTA 
ACCCCAGACC CAAAGATGTT GATTCAGTTG CCTTCTGCAT CGCAAAGTCA AGTTAACCCT 
TTGAGTTCTG TAAGTTGCAT GCCAACCAGG CCTCCAGGTC CAGAGGAAAG CAAGTCACAG 
GAGAGTATGG GCAGTTCTGC AAACCCGTTT CCCAGCCTGC CCTGCCGGAA CCCTTTTACA 
GACAGGACTG CTGCCCCTGG AAACCCATTT AGAGTTCAGT CCCAAGAATC AGAGGCAACT 
TCTTGGCTCT CCAAAGAAGA GCCTGTTCCC AACAGTCCCT TCCCTCCTCT CATGCCTCTC 
AGTCATGACA CGAGCAAGGC TTCAAGTTCG CTTGGTGGCT TTGAGGACAA TTTTGATCTG 
CAGAGCCAGT CTACAGTAAA AACAAGCAAC CCCAAAGGAT GGGTAACCTT TGACGAAGAC 
GACAACTTTC CTACAACAGG AAAGTCAAAG TCAGTTTGTC CAGACTTAGT GGGTAACGCG 
CCAGCTTCAT TTGATGATGA CTGGAGTAAA GGTGCGAGCG TTTCTTTCTG TGTGTTGCCA 
GCCAGAAGGC CACCACCACC ACCACCTCCT GTCCCTCTGC TACCACCTGG CACAACCTCA 
TCTGCAGGAC CTAGCACAAC CCTGCCATCT AAGGCACCTT CCACATTGGA CTTCACGGAA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244379.1
CDS	2226..7331
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246077.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246077.3

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGTGTTGG AGCCCCTCCC 
AGTCCCGGGG TTGCTAGGAG AGAGATAGAA GCACCCAAAA GCCCTGGAAC AGCACGGAAA 
GATAATATAG GGCGTAATCA GCCTTCCCCT CAAGCCGGAC TTGCAGGCCC AGGACCCGCT 
GGATACGGTG CGGCTAGACC GACAATTCCA GCTCGTGCTG GAGTGATCAG CGCCCCTCAG 
AGCCAGGCTC GGGTATGTGC TGGAAGGCCG ACTCCTGACA GCCAAAGCAA GCCCTCGGAG 
ACATTGAAAG GTCCTGCTGT CCTTCCAGAA CCACTGAAGC CTCAGGCTGC ATTTCCTCAG 
CAGCCTTCTC TGCCCACACC TGCTCAAAAG TTACAGGACC CGCTTGTCCC CATAGCAGCA 
CCTACTATGC CTCCCTCTGG CCCCCAACCA AATTTGGAAA CCCCCCCACA GCCCCCACCT 
CGGAGCAGGT CATCTCAAAG CTTGCCTTCA GACTCCTCAC CACAGCTGCA GGTAAAAATA 
AATGGCATTT CTGGTGTCAA ACAAGAGCCA ACATTGAAGA GTGACCCATT TGAAGATCTC 
TCACTTAGTG TGCTTGCTGT ATCAAAGGCT CAGCCTTCTG TTCAGATTTC ACCTGTTCTA 
ACCCCAGACC CAAAGATGTT GATTCAGTTG CCTTCTGCAT CGCAAAGTCA AGTTAACCCT 
TTGAGTTCTG TAAGTTGCAT GCCAACCAGG CCTCCAGGTC CAGAGGAAAG CAAGTCACAG 
GAGAGTATGG GCAGTTCTGC AAACCCGTTT CCCAGCCTGC CCTGCCGGAA CCCTTTTACA 
GACAGGACTG CTGCCCCTGG AAACCCATTT AGAGTTCAGT CCCAAGAATC AGAGGCAACT 
TCTTGGCTCT CCAAAGAAGA GCCTGTTCCC AACAGTCCCT TCCCTCCTCT CATGCCTCTC 
AGTCATGACA CGAGCAAGGC TTCAAGTTCG CTTGGTGGCT TTGAGGACAA TTTTGATCTG 
CAGAGCCAGT CTACAGTAAA AACAAGCAAC CCCAAAGGAT GGGTAACCTT TGACGAAGAC 
GACAACTTTC CTACAACAGG AAAGTCAAAG TCAGTTTGTC CAGACTTAGT GGGTAACGCG 
CCAGCTTCAT TTGATGATGA CTGGAGTAAA GGTGCGAGCG TTTCTTTCTG TGTGTTGCCA 
GCCAGAAGGC CACCACCACC ACCACCTCCT GTCCCTCTGC TACCACCTGG CACAACCTCA 
TCTGCAGGAC CTAGCACAAC CCTGCCATCT AAGGCACCTT CCACATTGGA CTTCACGGAA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246078.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244380.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X5, mRNA.

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CloneID	OMu72070
Clone ID Related Accession (Same CDS sequence)	XM_011246078.3 , XM_011246078.2
Accession Version	XM_011246078.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246078.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGCACCCAAA AGCCCTGGAA CAGCACGGAA AGATAATATA 
GGGCGTAATC AGCCTTCCCC TCAAGCCGGA CTTGCAGGCC CAGGACCCGC TGGATACGGT 
GCGGCTAGAC CGACAATTCC AGCTCGTGCT GGAGTGATCA GCGCCCCTCA GAGCCAGGCT 
CGGGTATGTG CTGGAAGGCC GACTCCTGAC AGCCAAAGCA AGCCCTCGGA GACATTGAAA 
GGTCCTGCTG TCCTTCCAGA ACCACTGAAG CCTCAGGCTG CATTTCCTCA GCAGCCTTCT 
CTGCCCACAC CTGCTCAAAA GTTACAGGAC CCGCTTGTCC CCATAGCAGC ACCTACTATG 
CCTCCCTCTG GCCCCCAACC AAATTTGGAA ACCCCCCCAC AGCCCCCACC TCGGAGCAGG 
TCATCTCAAA GCTTGCCTTC AGACTCCTCA CCACAGCTGC AGGTAAAAAT AAATGGCATT 
TCTGGTGTCA AACAAGAGCC AACATTGAAG AGTGACCCAT TTGAAGATCT CTCACTTAGT 
GTGCTTGCTG TATCAAAGGC TCAGCCTTCT GTTCAGATTT CACCTGTTCT AACCCCAGAC 
CCAAAGATGT TGATTCAGTT GCCTTCTGCA TCGCAAAGTC AAGTTAACCC TTTGAGTTCT 
GTAAGTTGCA TGCCAACCAG GCCTCCAGGT CCAGAGGAAA GCAAGTCACA GGAGAGTATG 
GGCAGTTCTG CAAACCCGTT TCCCAGCCTG CCCTGCCGGA ACCCTTTTAC AGACAGGACT 
GCTGCCCCTG GAAACCCATT TAGAGTTCAG TCCCAAGAAT CAGAGGCAAC TTCTTGGCTC 
TCCAAAGAAG AGCCTGTTCC CAACAGTCCC TTCCCTCCTC TCATGCCTCT CAGTCATGAC 
ACGAGCAAGG CTTCAAGTTC GCTTGGTGGC TTTGAGGACA ATTTTGATCT GCAGAGCCAG 
TCTACAGTAA AAACAAGCAA CCCCAAAGGA TGGGTAACCT TTGACGAAGA CGACAACTTT 
CCTACAACAG GAAAGTCAAA GTCAGTTTGT CCAGACTTAG TGGGTAACGC GCCAGCTTCA 
TTTGATGATG ACTGGAGTAA AGGTGCGAGC GTTTCTTTCT GTGTGTTGCC AGCCAGAAGG 
CCACCACCAC CACCACCTCC TGTCCCTCTG CTACCACCTG GCACAACCTC ATCTGCAGGA 
CCTAGCACAA CCCTGCCATC TAAGGCACCT TCCACATTGG ACTTCACGGA AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244380.1
CDS	2226..7322
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246078.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246078.3

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGCACCCAAA AGCCCTGGAA CAGCACGGAA AGATAATATA 
GGGCGTAATC AGCCTTCCCC TCAAGCCGGA CTTGCAGGCC CAGGACCCGC TGGATACGGT 
GCGGCTAGAC CGACAATTCC AGCTCGTGCT GGAGTGATCA GCGCCCCTCA GAGCCAGGCT 
CGGGTATGTG CTGGAAGGCC GACTCCTGAC AGCCAAAGCA AGCCCTCGGA GACATTGAAA 
GGTCCTGCTG TCCTTCCAGA ACCACTGAAG CCTCAGGCTG CATTTCCTCA GCAGCCTTCT 
CTGCCCACAC CTGCTCAAAA GTTACAGGAC CCGCTTGTCC CCATAGCAGC ACCTACTATG 
CCTCCCTCTG GCCCCCAACC AAATTTGGAA ACCCCCCCAC AGCCCCCACC TCGGAGCAGG 
TCATCTCAAA GCTTGCCTTC AGACTCCTCA CCACAGCTGC AGGTAAAAAT AAATGGCATT 
TCTGGTGTCA AACAAGAGCC AACATTGAAG AGTGACCCAT TTGAAGATCT CTCACTTAGT 
GTGCTTGCTG TATCAAAGGC TCAGCCTTCT GTTCAGATTT CACCTGTTCT AACCCCAGAC 
CCAAAGATGT TGATTCAGTT GCCTTCTGCA TCGCAAAGTC AAGTTAACCC TTTGAGTTCT 
GTAAGTTGCA TGCCAACCAG GCCTCCAGGT CCAGAGGAAA GCAAGTCACA GGAGAGTATG 
GGCAGTTCTG CAAACCCGTT TCCCAGCCTG CCCTGCCGGA ACCCTTTTAC AGACAGGACT 
GCTGCCCCTG GAAACCCATT TAGAGTTCAG TCCCAAGAAT CAGAGGCAAC TTCTTGGCTC 
TCCAAAGAAG AGCCTGTTCC CAACAGTCCC TTCCCTCCTC TCATGCCTCT CAGTCATGAC 
ACGAGCAAGG CTTCAAGTTC GCTTGGTGGC TTTGAGGACA ATTTTGATCT GCAGAGCCAG 
TCTACAGTAA AAACAAGCAA CCCCAAAGGA TGGGTAACCT TTGACGAAGA CGACAACTTT 
CCTACAACAG GAAAGTCAAA GTCAGTTTGT CCAGACTTAG TGGGTAACGC GCCAGCTTCA 
TTTGATGATG ACTGGAGTAA AGGTGCGAGC GTTTCTTTCT GTGTGTTGCC AGCCAGAAGG 
CCACCACCAC CACCACCTCC TGTCCCTCTG CTACCACCTG GCACAACCTC ATCTGCAGGA 
CCTAGCACAA CCCTGCCATC TAAGGCACCT TCCACATTGG ACTTCACGGA AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246079.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244381.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X7, mRNA.

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CloneID	OMu72071
Clone ID Related Accession (Same CDS sequence)	XM_011246079.3 , XM_011246079.2
Accession Version	XM_011246079.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246079.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGCACCCAA AAGCCCTGGA 
ACAGCACGGA AAGATAATAT AGGGCGTAAT CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC 
CCAGGACCCG CTGGATACGG TGCGGCTAGA CCGACAATTC CAGCTCGTGC TGGAGTGATC 
AGCGCCCCTC AGAGCCAGGC TCGGGTATGT GCTGGAAGGC CGACTCCTGA CAGCCAAAGC 
AAGCCCTCGG AGACATTGAA AGGTCCTGCT GTCCTTCCAG AACCACTGAA GCCTCAGGCT 
GCATTTCCTC AGCAGCCTTC TCTGCCCACA CCTGCTCAAA AGTTACAGGA CCCGCTTGTC 
CCCATAGCAG CACCTACTAT GCCTCCCTCT GGCCCCCAAC CAAATTTGGA AACCCCCCCA 
CAGCCCCCAC CTCGGAGCAG GTCATCTCAA AGCTTGCCTT CAGACTCCTC ACCACAGCTG 
CAGGTAAAAA TAAATGGCAT TTCTGGTGTC AAACAAGAGC CAACATTGAA GAGTGACCCA 
TTTGAAGATC TCTCACTTAG TGTGCTTGCT GTATCAAAGG CTCAGCCTTC TGTTCAGATT 
TCACCTGTTC TAACCCCAGA CCCAAAGATG TTGATTCAGT TGCCTTCTGC ATCGCAAAGT 
CAAGTTAACC CTTTGAGTTC TGTAAGTTGC ATGCCAACCA GGCCTCCAGG TCCAGAGGAA 
AGCAAGTCAC AGGAGAGTAT GGGCAGTTCT GCAAACCCGT TTCCCAGCCT GCCCTGCCGG 
AACCCTTTTA CAGACAGGAC TGCTGCCCCT GGAAACCCAT TTAGAGTTCA GTCCCAAGAA 
TCAGAGGCAA CTTCTTGGCT CTCCAAAGAA GAGCCTGTTC CCAACAGTCC CTTCCCTCCT 
CTCATGCCTC TCAGTCATGA CACGAGCAAG GCTTCAAGTT CGCTTGGTGG CTTTGAGGAC 
AATTTTGATC TGCAGAGCCA GTCTACAGTA AAAACAAGCA ACCCCAAAGG ATGGGTAACC 
TTTGACGAAG ACGACAACTT TCCTACAACA GGAAAGTCAA AGTCAGTTTG TCCAGACTTA 
GTGGGTAACG CGCCAGCTTC ATTTGATGAT GACTGGAGTA AAGGTGCGAG CGTTTCTTTC 
TGTGTGTTGC CAGCCAGAAG GCCACCACCA CCACCACCTC CTGTCCCTCT GCTACCACCT 
GGCACAACCT CATCTGCAGG ACCTAGCACA ACCCTGCCAT CTAAGGCACC TTCCACATTG 
GACTTCACGG AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244381.1
CDS	2226..7283
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246079.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246079.3

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGCACCCAA AAGCCCTGGA 
ACAGCACGGA AAGATAATAT AGGGCGTAAT CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC 
CCAGGACCCG CTGGATACGG TGCGGCTAGA CCGACAATTC CAGCTCGTGC TGGAGTGATC 
AGCGCCCCTC AGAGCCAGGC TCGGGTATGT GCTGGAAGGC CGACTCCTGA CAGCCAAAGC 
AAGCCCTCGG AGACATTGAA AGGTCCTGCT GTCCTTCCAG AACCACTGAA GCCTCAGGCT 
GCATTTCCTC AGCAGCCTTC TCTGCCCACA CCTGCTCAAA AGTTACAGGA CCCGCTTGTC 
CCCATAGCAG CACCTACTAT GCCTCCCTCT GGCCCCCAAC CAAATTTGGA AACCCCCCCA 
CAGCCCCCAC CTCGGAGCAG GTCATCTCAA AGCTTGCCTT CAGACTCCTC ACCACAGCTG 
CAGGTAAAAA TAAATGGCAT TTCTGGTGTC AAACAAGAGC CAACATTGAA GAGTGACCCA 
TTTGAAGATC TCTCACTTAG TGTGCTTGCT GTATCAAAGG CTCAGCCTTC TGTTCAGATT 
TCACCTGTTC TAACCCCAGA CCCAAAGATG TTGATTCAGT TGCCTTCTGC ATCGCAAAGT 
CAAGTTAACC CTTTGAGTTC TGTAAGTTGC ATGCCAACCA GGCCTCCAGG TCCAGAGGAA 
AGCAAGTCAC AGGAGAGTAT GGGCAGTTCT GCAAACCCGT TTCCCAGCCT GCCCTGCCGG 
AACCCTTTTA CAGACAGGAC TGCTGCCCCT GGAAACCCAT TTAGAGTTCA GTCCCAAGAA 
TCAGAGGCAA CTTCTTGGCT CTCCAAAGAA GAGCCTGTTC CCAACAGTCC CTTCCCTCCT 
CTCATGCCTC TCAGTCATGA CACGAGCAAG GCTTCAAGTT CGCTTGGTGG CTTTGAGGAC 
AATTTTGATC TGCAGAGCCA GTCTACAGTA AAAACAAGCA ACCCCAAAGG ATGGGTAACC 
TTTGACGAAG ACGACAACTT TCCTACAACA GGAAAGTCAA AGTCAGTTTG TCCAGACTTA 
GTGGGTAACG CGCCAGCTTC ATTTGATGAT GACTGGAGTA AAGGTGCGAG CGTTTCTTTC 
TGTGTGTTGC CAGCCAGAAG GCCACCACCA CCACCACCTC CTGTCCCTCT GCTACCACCT 
GGCACAACCT CATCTGCAGG ACCTAGCACA ACCCTGCCAT CTAAGGCACC TTCCACATTG 
GACTTCACGG AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246080.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244382.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X8, mRNA.

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CloneID	OMu72072
Clone ID Related Accession (Same CDS sequence)	XM_011246080.2 , XM_011246080.3
Accession Version	XM_011246080.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246080.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGGCGTAAT CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC 
CCAGGACCCG CTGGATACGG TGCGGCTAGA CCGACAATTC CAGCTCGTGC TGGAGTGATC 
AGCGCCCCTC AGAGCCAGGC TCGGGTATGT GCTGGAAGGC CGACTCCTGA CAGCCAAAGC 
AAGCCCTCGG AGACATTGAA AGGTCCTGCT GTCCTTCCAG AACCACTGAA GCCTCAGGCT 
GCATTTCCTC AGCAGCCTTC TCTGCCCACA CCTGCTCAAA AGTTACAGGA CCCGCTTGTC 
CCCATAGCAG CACCTACTAT GCCTCCCTCT GGCCCCCAAC CAAATTTGGA AACCCCCCCA 
CAGCCCCCAC CTCGGAGCAG GTCATCTCAA AGCTTGCCTT CAGACTCCTC ACCACAGCTG 
CAGGTAAAAA TAAATGGCAT TTCTGGTGTC AAACAAGAGC CAACATTGAA GAGTGACCCA 
TTTGAAGATC TCTCACTTAG TGTGCTTGCT GTATCAAAGG CTCAGCCTTC TGTTCAGATT 
TCACCTGTTC TAACCCCAGA CCCAAAGATG TTGATTCAGT TGCCTTCTGC ATCGCAAAGT 
CAAGTTAACC CTTTGAGTTC TGTAAGTTGC ATGCCAACCA GGCCTCCAGG TCCAGAGGAA 
AGCAAGTCAC AGGAGAGTAT GGGCAGTTCT GCAAACCCGT TTCCCAGCCT GCCCTGCCGG 
AACCCTTTTA CAGACAGGAC TGCTGCCCCT GGAAACCCAT TTAGAGTTCA GTCCCAAGAA 
TCAGAGGCAA CTTCTTGGCT CTCCAAAGAA GAGCCTGTTC CCAACAGTCC CTTCCCTCCT 
CTCATGCCTC TCAGTCATGA CACGAGCAAG GCTTCAAGTT CGCTTGGTGG CTTTGAGGAC 
AATTTTGATC TGCAGAGCCA GTCTACAGTA AAAACAAGCA ACCCCAAAGG ATGGGTAACC 
TTTGACGAAG ACGACAACTT TCCTACAACA GGAAAGTCAA AGTCAGTTTG TCCAGACTTA 
GTGGGTAACG CGCCAGCTTC ATTTGATGAT GACTGGAGTA AAGGTGCGAG CGTTTCTTTC 
TGTGTGTTGC CAGCCAGAAG GCCACCACCA CCACCACCTC CTGTCCCTCT GCTACCACCT 
GGCACAACCT CATCTGCAGG ACCTAGCACA ACCCTGCCAT CTAAGGCACC TTCCACATTG 
GACTTCACGG AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244382.1
CDS	2226..7283
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246080.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246080.3

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGGCGTAAT CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC 
CCAGGACCCG CTGGATACGG TGCGGCTAGA CCGACAATTC CAGCTCGTGC TGGAGTGATC 
AGCGCCCCTC AGAGCCAGGC TCGGGTATGT GCTGGAAGGC CGACTCCTGA CAGCCAAAGC 
AAGCCCTCGG AGACATTGAA AGGTCCTGCT GTCCTTCCAG AACCACTGAA GCCTCAGGCT 
GCATTTCCTC AGCAGCCTTC TCTGCCCACA CCTGCTCAAA AGTTACAGGA CCCGCTTGTC 
CCCATAGCAG CACCTACTAT GCCTCCCTCT GGCCCCCAAC CAAATTTGGA AACCCCCCCA 
CAGCCCCCAC CTCGGAGCAG GTCATCTCAA AGCTTGCCTT CAGACTCCTC ACCACAGCTG 
CAGGTAAAAA TAAATGGCAT TTCTGGTGTC AAACAAGAGC CAACATTGAA GAGTGACCCA 
TTTGAAGATC TCTCACTTAG TGTGCTTGCT GTATCAAAGG CTCAGCCTTC TGTTCAGATT 
TCACCTGTTC TAACCCCAGA CCCAAAGATG TTGATTCAGT TGCCTTCTGC ATCGCAAAGT 
CAAGTTAACC CTTTGAGTTC TGTAAGTTGC ATGCCAACCA GGCCTCCAGG TCCAGAGGAA 
AGCAAGTCAC AGGAGAGTAT GGGCAGTTCT GCAAACCCGT TTCCCAGCCT GCCCTGCCGG 
AACCCTTTTA CAGACAGGAC TGCTGCCCCT GGAAACCCAT TTAGAGTTCA GTCCCAAGAA 
TCAGAGGCAA CTTCTTGGCT CTCCAAAGAA GAGCCTGTTC CCAACAGTCC CTTCCCTCCT 
CTCATGCCTC TCAGTCATGA CACGAGCAAG GCTTCAAGTT CGCTTGGTGG CTTTGAGGAC 
AATTTTGATC TGCAGAGCCA GTCTACAGTA AAAACAAGCA ACCCCAAAGG ATGGGTAACC 
TTTGACGAAG ACGACAACTT TCCTACAACA GGAAAGTCAA AGTCAGTTTG TCCAGACTTA 
GTGGGTAACG CGCCAGCTTC ATTTGATGAT GACTGGAGTA AAGGTGCGAG CGTTTCTTTC 
TGTGTGTTGC CAGCCAGAAG GCCACCACCA CCACCACCTC CTGTCCCTCT GCTACCACCT 
GGCACAACCT CATCTGCAGG ACCTAGCACA ACCCTGCCAT CTAAGGCACC TTCCACATTG 
GACTTCACGG AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246081.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244383.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X11, mRNA.

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CloneID	OMu72073
Clone ID Related Accession (Same CDS sequence)	XM_011246081.2 , XM_011246081.3
Accession Version	XM_011246081.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246081.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGCGTAA TCAGCCTTCC 
CCTCAAGCCG GACTTGCAGG CCCAGGACCC GCTGGATACG GTGCGGCTAG ACCGACAATT 
CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT CAGAGCCAGG CTCGGGTATG TGCTGGAAGG 
CCGACTCCTG ACAGCCAAAG CAAGCCCTCG GAGACATTGA AAGGTCCTGC TGTCCTTCCA 
GAACCACTGA AGCCTCAGGC TGCATTTCCT CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA 
AAGTTACAGG ACCCGCTTGT CCCCATAGCA GCACCTACTA TGCCTCCCTC TGGCCCCCAA 
CCAAATTTGG AAACCCCCCC ACAGCCCCCA CCTCGGAGCA GGTCATCTCA AAGCTTGCCT 
TCAGACTCCT CACCACAGCT GCAGGTAAAA ATAAATGGCA TTTCTGGTGT CAAACAAGAG 
CCAACATTGA AGAGTGACCC ATTTGAAGAT CTCTCACTTA GTGTGCTTGC TGTATCAAAG 
GCTCAGCCTT CTGTTCAGAT TTCACCTGTT CTAACCCCAG ACCCAAAGAT GTTGATTCAG 
TTGCCTTCTG CATCGCAAAG TCAAGTTAAC CCTTTGAGTT CTGTAAGTTG CATGCCAACC 
AGGCCTCCAG GTCCAGAGGA AAGCAAGTCA CAGGAGAGTA TGGGCAGTTC TGCAAACCCG 
TTTCCCAGCC TGCCCTGCCG GAACCCTTTT ACAGACAGGA CTGCTGCCCC TGGAAACCCA 
TTTAGAGTTC AGTCCCAAGA ATCAGAGGCA ACTTCTTGGC TCTCCAAAGA AGAGCCTGTT 
CCCAACAGTC CCTTCCCTCC TCTCATGCCT CTCAGTCATG ACACGAGCAA GGCTTCAAGT 
TCGCTTGGTG GCTTTGAGGA CAATTTTGAT CTGCAGAGCC AGTCTACAGT AAAAACAAGC 
AACCCCAAAG GATGGGTAAC CTTTGACGAA GACGACAACT TTCCTACAAC AGGAAAGTCA 
AAGTCAGTTT GTCCAGACTT AGTGGGTAAC GCGCCAGCTT CATTTGATGA TGACTGGAGT 
AAAGGTGCGA GCGTTTCTTT CTGTGTGTTG CCAGCCAGAA GGCCACCACC ACCACCACCT 
CCTGTCCCTC TGCTACCACC TGGCACAACC TCATCTGCAG GACCTAGCAC AACCCTGCCA 
TCTAAGGCAC CTTCCACATT GGACTTCACG GAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244383.1
CDS	2226..7244
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246081.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246081.3

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGCGTAA TCAGCCTTCC 
CCTCAAGCCG GACTTGCAGG CCCAGGACCC GCTGGATACG GTGCGGCTAG ACCGACAATT 
CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT CAGAGCCAGG CTCGGGTATG TGCTGGAAGG 
CCGACTCCTG ACAGCCAAAG CAAGCCCTCG GAGACATTGA AAGGTCCTGC TGTCCTTCCA 
GAACCACTGA AGCCTCAGGC TGCATTTCCT CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA 
AAGTTACAGG ACCCGCTTGT CCCCATAGCA GCACCTACTA TGCCTCCCTC TGGCCCCCAA 
CCAAATTTGG AAACCCCCCC ACAGCCCCCA CCTCGGAGCA GGTCATCTCA AAGCTTGCCT 
TCAGACTCCT CACCACAGCT GCAGGTAAAA ATAAATGGCA TTTCTGGTGT CAAACAAGAG 
CCAACATTGA AGAGTGACCC ATTTGAAGAT CTCTCACTTA GTGTGCTTGC TGTATCAAAG 
GCTCAGCCTT CTGTTCAGAT TTCACCTGTT CTAACCCCAG ACCCAAAGAT GTTGATTCAG 
TTGCCTTCTG CATCGCAAAG TCAAGTTAAC CCTTTGAGTT CTGTAAGTTG CATGCCAACC 
AGGCCTCCAG GTCCAGAGGA AAGCAAGTCA CAGGAGAGTA TGGGCAGTTC TGCAAACCCG 
TTTCCCAGCC TGCCCTGCCG GAACCCTTTT ACAGACAGGA CTGCTGCCCC TGGAAACCCA 
TTTAGAGTTC AGTCCCAAGA ATCAGAGGCA ACTTCTTGGC TCTCCAAAGA AGAGCCTGTT 
CCCAACAGTC CCTTCCCTCC TCTCATGCCT CTCAGTCATG ACACGAGCAA GGCTTCAAGT 
TCGCTTGGTG GCTTTGAGGA CAATTTTGAT CTGCAGAGCC AGTCTACAGT AAAAACAAGC 
AACCCCAAAG GATGGGTAAC CTTTGACGAA GACGACAACT TTCCTACAAC AGGAAAGTCA 
AAGTCAGTTT GTCCAGACTT AGTGGGTAAC GCGCCAGCTT CATTTGATGA TGACTGGAGT 
AAAGGTGCGA GCGTTTCTTT CTGTGTGTTG CCAGCCAGAA GGCCACCACC ACCACCACCT 
CCTGTCCCTC TGCTACCACC TGGCACAACC TCATCTGCAG GACCTAGCAC AACCCTGCCA 
TCTAAGGCAC CTTCCACATT GGACTTCACG GAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246082.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244384.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X12, mRNA.

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CloneID	OMu72074
Clone ID Related Accession (Same CDS sequence)	XM_011246082.3 , XM_011246082.2
Accession Version	XM_011246082.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246082.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GCATCTTCCA AAGTCTTAAA GAACATGTGT 
GAGAACTTCT ACAAATACTC AAAGCCCAAG AAGATCCGAG TGTGTGTTGG CACCTGGAAT 
GTGAACGGCG GGAAGCAGTT CCGCAGCATA GCGTTCAAGA ACCAGACGCT CACAGACTGG 
CTTCTGGACG CTCCCAAGTT AGCCGGCATC CAGGAGTTTC AAGATAAGAG AAGTAAGCCA 
ACTGATATAT TTGCAATTGG CTTTGAAGAA ATGGTGGAGC TGAATGCTGG GAACATTGTG 
AATGCAAGCA CAACCAACCA GAAGCTGTGG GCAGTGGAGC TGCAGAAGAC CATCTCCAGG 
GACAACAAGT ATGTCCTGCT GGCCTCGGAG CAGCTGGTGG GTGTCTGCCT GTTTGTCTTT 
ATCAGACCAC AGCACGCTCC TTTTATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGC 
ATGGGAGGTG CAACTGGAAA CAAGGGCGCC GTTGCAATTC GGATGCTCTT CCACACCACC 
AGCCTTTGCT TCGTCTGCAG CCACTTTGCT GCAGGGCAGT CCCAAGTCAA AGAGAGAAAT 
GAAGATTTTG TAGAAATAGC ACGAAAACTG AGTTTCCCCA TGGGACGAAT GCTCTTCTCC 
CATGACTATG TATTTTGGTG TGGTGATTTC AACTATCGAA TTGACCTTCC GAATGAAGAG 
GTTAAAGAGC TCATAAGACA ACAAAACTGG GATTCTCTGA TCGCTGGGGA TCAGCTCATC 
AACCAGAAAA ATGCAGGACA GATCTTTAGA GGATTCTTAG AGGGAAAAGT AACGTTTGCT 
CCAACCTATA AATATGACTT GTTTTCTGAA GACTATGACA CCAGTGAGAA GTGTCGCACC 
CCTGCTTGGA CAGACCGTGT CCTCTGGAGA AGGAGGAAGT GGCCTTTTGA CAGATCAGCT 
GAAGATTTAG ATCTCCTGAA TGCTAGTTTC CAAGATGAAA GTAAAATTCT TTATACATGG 
ACCCCTGGCA CCTTGCTGCA CTATGGAAGA GCCGAGCTGA AGACTTCTGA CCATAGGCCC 
GTCGTTGCCT TGATTGACAT AGATATATTT GAAGTTGAAG CTGAAGAGAG ACAAAAAATT 
TATAAAGAAG TTATTGCCGT CCAGGGCCCA CCAGATGGCA CAGTGCTGGT CTCAATCAAA 
AGTTCTGCAC AAGAAAGTAC TTTTTTTGAT GATGCTTTGA TTGATGAGCT TCTGCGGCAG 
TTTGCACACT TTGGTGAAGT TATACTCATA AGATTTGTAG AAGATAAAAT GTGGGTTACA 
TTTTTGGAGG GAAGCTCTGC CTTGAATGCT CTGAGCCTAA ATGGGAAAGA GCTATTAAAT 
CGGACTATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AACATTTGGA AGAAGAGATG 
AGTTTAGAGA AAATCAGTGT CACGTTGCCC TCATCAGCAA GCTCCACCCT GCTTGGTGAA 
GACGCAGAGG TCGCAGCAGA CTTTGACATG GAAGGTGACG TCGATGACTA CAGCGCTGAA 
GTAGAGGAGC TTCTTCCTCA GCATCTGCAG CCGTCCTCAA GTTCTGGCCT GGGCACTTCT 
CCAAGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCCCCCA CAGTACCAGA GTACTCCGCC 
CCTTCTCTTC CCATCAGACC TAGCCGAGCA CCGTCAAGAA CTCCAGGACC TCCAAGTTCG 
CAGGGCTCTC CAGTTGACAC TCAGCCAGCG GCCCAGAAGG ATTCTTCCCA GACTCTAGAG 
CCCAAGAGAC CACCCCCTCC CCGCCCAGTC GCTCCTCCGG CACGTCCTGC CCCACCACAG 
AGACCACCTC CACCTTCAGG GCGTAATCAG CCTTCCCCTC AAGCCGGACT TGCAGGCCCA 
GGACCCGCTG GATACGGTGC GGCTAGACCG ACAATTCCAG CTCGTGCTGG AGTGATCAGC 
GCCCCTCAGA GCCAGGCTCG GGTATGTGCT GGAAGGCCGA CTCCTGACAG CCAAAGCAAG 
CCCTCGGAGA CATTGAAAGG TCCTGCTGTC CTTCCAGAAC CACTGAAGCC TCAGGCTGCA 
TTTCCTCAGC AGCCTTCTCT GCCCACACCT GCTCAAAAGT TACAGGACCC GCTTGTCCCC 
ATAGCAGCAC CTACTATGCC TCCCTCTGGC CCCCAACCAA ATTTGGAAAC CCCCCCACAG 
CCCCCACCTC GGAGCAGGTC ATCTCAAAGC TTGCCTTCAG ACTCCTCACC ACAGCTGCAG 
GTAAAAATAA ATGGCATTTC TGGTGTCAAA CAAGAGCCAA CATTGAAGAG TGACCCATTT 
GAAGATCTCT CACTTAGTGT GCTTGCTGTA TCAAAGGCTC AGCCTTCTGT TCAGATTTCA 
CCTGTTCTAA CCCCAGACCC AAAGATGTTG ATTCAGTTGC CTTCTGCATC GCAAAGTCAA 
GTTAACCCTT TGAGTTCTGT AAGTTGCATG CCAACCAGGC CTCCAGGTCC AGAGGAAAGC 
AAGTCACAGG AGAGTATGGG CAGTTCTGCA AACCCGTTTC CCAGCCTGCC CTGCCGGAAC 
CCTTTTACAG ACAGGACTGC TGCCCCTGGA AACCCATTTA GAGTTCAGTC CCAAGAATCA 
GAGGCAACTT CTTGGCTCTC CAAAGAAGAG CCTGTTCCCA ACAGTCCCTT CCCTCCTCTC 
ATGCCTCTCA GTCATGACAC GAGCAAGGCT TCAAGTTCGC TTGGTGGCTT TGAGGACAAT 
TTTGATCTGC AGAGCCAGTC TACAGTAAAA ACAAGCAACC CCAAAGGATG GGTAACCTTT 
GACGAAGACG ACAACTTTCC TACAACAGGA AAGTCAAAGT CAGTTTGTCC AGACTTAGTG 
GGTAACGCGC CAGCTTCATT TGATGATGAC TGGAGTAAAG GTGCGAGCGT TTCTTTCTGT 
GTGTTGCCAG CCAGAAGGCC ACCACCACCA CCACCTCCTG TCCCTCTGCT ACCACCTGGC 
ACAACCTCAT CTGCAGGACC TAGCACAACC CTGCCATCTA AGGCACCTTC CACATTGGAC 
TTCACGGAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244384.1
CDS	2226..7220
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246082.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246082.3

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GCATCTTCCA AAGTCTTAAA GAACATGTGT 
GAGAACTTCT ACAAATACTC AAAGCCCAAG AAGATCCGAG TGTGTGTTGG CACCTGGAAT 
GTGAACGGCG GGAAGCAGTT CCGCAGCATA GCGTTCAAGA ACCAGACGCT CACAGACTGG 
CTTCTGGACG CTCCCAAGTT AGCCGGCATC CAGGAGTTTC AAGATAAGAG AAGTAAGCCA 
ACTGATATAT TTGCAATTGG CTTTGAAGAA ATGGTGGAGC TGAATGCTGG GAACATTGTG 
AATGCAAGCA CAACCAACCA GAAGCTGTGG GCAGTGGAGC TGCAGAAGAC CATCTCCAGG 
GACAACAAGT ATGTCCTGCT GGCCTCGGAG CAGCTGGTGG GTGTCTGCCT GTTTGTCTTT 
ATCAGACCAC AGCACGCTCC TTTTATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGC 
ATGGGAGGTG CAACTGGAAA CAAGGGCGCC GTTGCAATTC GGATGCTCTT CCACACCACC 
AGCCTTTGCT TCGTCTGCAG CCACTTTGCT GCAGGGCAGT CCCAAGTCAA AGAGAGAAAT 
GAAGATTTTG TAGAAATAGC ACGAAAACTG AGTTTCCCCA TGGGACGAAT GCTCTTCTCC 
CATGACTATG TATTTTGGTG TGGTGATTTC AACTATCGAA TTGACCTTCC GAATGAAGAG 
GTTAAAGAGC TCATAAGACA ACAAAACTGG GATTCTCTGA TCGCTGGGGA TCAGCTCATC 
AACCAGAAAA ATGCAGGACA GATCTTTAGA GGATTCTTAG AGGGAAAAGT AACGTTTGCT 
CCAACCTATA AATATGACTT GTTTTCTGAA GACTATGACA CCAGTGAGAA GTGTCGCACC 
CCTGCTTGGA CAGACCGTGT CCTCTGGAGA AGGAGGAAGT GGCCTTTTGA CAGATCAGCT 
GAAGATTTAG ATCTCCTGAA TGCTAGTTTC CAAGATGAAA GTAAAATTCT TTATACATGG 
ACCCCTGGCA CCTTGCTGCA CTATGGAAGA GCCGAGCTGA AGACTTCTGA CCATAGGCCC 
GTCGTTGCCT TGATTGACAT AGATATATTT GAAGTTGAAG CTGAAGAGAG ACAAAAAATT 
TATAAAGAAG TTATTGCCGT CCAGGGCCCA CCAGATGGCA CAGTGCTGGT CTCAATCAAA 
AGTTCTGCAC AAGAAAGTAC TTTTTTTGAT GATGCTTTGA TTGATGAGCT TCTGCGGCAG 
TTTGCACACT TTGGTGAAGT TATACTCATA AGATTTGTAG AAGATAAAAT GTGGGTTACA 
TTTTTGGAGG GAAGCTCTGC CTTGAATGCT CTGAGCCTAA ATGGGAAAGA GCTATTAAAT 
CGGACTATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AACATTTGGA AGAAGAGATG 
AGTTTAGAGA AAATCAGTGT CACGTTGCCC TCATCAGCAA GCTCCACCCT GCTTGGTGAA 
GACGCAGAGG TCGCAGCAGA CTTTGACATG GAAGGTGACG TCGATGACTA CAGCGCTGAA 
GTAGAGGAGC TTCTTCCTCA GCATCTGCAG CCGTCCTCAA GTTCTGGCCT GGGCACTTCT 
CCAAGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCCCCCA CAGTACCAGA GTACTCCGCC 
CCTTCTCTTC CCATCAGACC TAGCCGAGCA CCGTCAAGAA CTCCAGGACC TCCAAGTTCG 
CAGGGCTCTC CAGTTGACAC TCAGCCAGCG GCCCAGAAGG ATTCTTCCCA GACTCTAGAG 
CCCAAGAGAC CACCCCCTCC CCGCCCAGTC GCTCCTCCGG CACGTCCTGC CCCACCACAG 
AGACCACCTC CACCTTCAGG GCGTAATCAG CCTTCCCCTC AAGCCGGACT TGCAGGCCCA 
GGACCCGCTG GATACGGTGC GGCTAGACCG ACAATTCCAG CTCGTGCTGG AGTGATCAGC 
GCCCCTCAGA GCCAGGCTCG GGTATGTGCT GGAAGGCCGA CTCCTGACAG CCAAAGCAAG 
CCCTCGGAGA CATTGAAAGG TCCTGCTGTC CTTCCAGAAC CACTGAAGCC TCAGGCTGCA 
TTTCCTCAGC AGCCTTCTCT GCCCACACCT GCTCAAAAGT TACAGGACCC GCTTGTCCCC 
ATAGCAGCAC CTACTATGCC TCCCTCTGGC CCCCAACCAA ATTTGGAAAC CCCCCCACAG 
CCCCCACCTC GGAGCAGGTC ATCTCAAAGC TTGCCTTCAG ACTCCTCACC ACAGCTGCAG 
GTAAAAATAA ATGGCATTTC TGGTGTCAAA CAAGAGCCAA CATTGAAGAG TGACCCATTT 
GAAGATCTCT CACTTAGTGT GCTTGCTGTA TCAAAGGCTC AGCCTTCTGT TCAGATTTCA 
CCTGTTCTAA CCCCAGACCC AAAGATGTTG ATTCAGTTGC CTTCTGCATC GCAAAGTCAA 
GTTAACCCTT TGAGTTCTGT AAGTTGCATG CCAACCAGGC CTCCAGGTCC AGAGGAAAGC 
AAGTCACAGG AGAGTATGGG CAGTTCTGCA AACCCGTTTC CCAGCCTGCC CTGCCGGAAC 
CCTTTTACAG ACAGGACTGC TGCCCCTGGA AACCCATTTA GAGTTCAGTC CCAAGAATCA 
GAGGCAACTT CTTGGCTCTC CAAAGAAGAG CCTGTTCCCA ACAGTCCCTT CCCTCCTCTC 
ATGCCTCTCA GTCATGACAC GAGCAAGGCT TCAAGTTCGC TTGGTGGCTT TGAGGACAAT 
TTTGATCTGC AGAGCCAGTC TACAGTAAAA ACAAGCAACC CCAAAGGATG GGTAACCTTT 
GACGAAGACG ACAACTTTCC TACAACAGGA AAGTCAAAGT CAGTTTGTCC AGACTTAGTG 
GGTAACGCGC CAGCTTCATT TGATGATGAC TGGAGTAAAG GTGCGAGCGT TTCTTTCTGT 
GTGTTGCCAG CCAGAAGGCC ACCACCACCA CCACCTCCTG TCCCTCTGCT ACCACCTGGC 
ACAACCTCAT CTGCAGGACC TAGCACAACC CTGCCATCTA AGGCACCTTC CACATTGGAC 
TTCACGGAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_017316836.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172325.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu79239
Clone ID Related Accession (Same CDS sequence)	XM_017316836.2 , XM_017316836.1
Accession Version	XM_017316836.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316836.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGGGCGTAA TCAGCCTTCC CCTCAAGCCG GACTTGCAGG CCCAGGACCC 
GCTGGATACG GTGCGGCTAG ACCGACAATT CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT 
CAGAGCCAGG CTCGGGTATG TGCTGGAAGG CCGACTCCTG ACAGCCAAAG CAAGCCCTCG 
GAGACATTGA AAGGTCCTGC TGTCCTTCCA GAACCACTGA AGCCTCAGGC TGCATTTCCT 
CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA AAGTTACAGG ACCCGCTTGT CCCCATAGCA 
GCACCTACTA TGCCTCCCTC TGGCCCCCAA CCAAATTTGG AAACCCCCCC ACAGCCCCCA 
CCTCGGAGCA GGTCATCTCA AAGCTTGCCT TCAGACTCCT CACCACAGCT GCAGGTAAAA 
ATAAATGGCA TTTCTGGTGT CAAACAAGAG CCAACATTGA AGAGTGACCC ATTTGAAGAT 
CTCTCACTTA GTGTGCTTGC TGTATCAAAG GCTCAGCCTT CTGTTCAGAT TTCACCTGTT 
CTAACCCCAG ACCCAAAGAT GTTGATTCAG TTGCCTTCTG CATCGCAAAG TCAAGTTAAC 
CCTTTGAGTT CTGTAAGTTG CATGCCAACC AGGCCTCCAG GTCCAGAGGA AAGCAAGTCA 
CAGGAGAGTA TGGGCAGTTC TGCAAACCCG TTTCCCAGCC TGCCCTGCCG GAACCCTTTT 
ACAGACAGGA CTGCTGCCCC TGGAAACCCA TTTAGAGTTC AGTCCCAAGA ATCAGAGGCA 
ACTTCTTGGC TCTCCAAAGA AGAGCCTGTT CCCAACAGTC CCTTCCCTCC TCTCATGCCT 
CTCAGTCATG ACACGAGCAA GGCTTCAAGT TCGCTTGGTG GCTTTGAGGA CAATTTTGAT 
CTGCAGAGCC AGTCTACAGT AAAAACAAGC AACCCCAAAG GATGGGTAAC CTTTGACGAA 
GACGACAACT TTCCTACAAC AGGAAAGTCA AAGTCAGTTT GTCCAGACTT AGTGGGTAAC 
GCGCCAGCTT CATTTGATGA TGACTGGAGT AAAGGTGCGA GCGTTTCTTT CTGTGTGTTG 
CCAGCCAGAA GGCCACCACC ACCACCACCT CCTGTCCCTC TGCTACCACC TGGCACAACC 
TCATCTGCAG GACCTAGCAC AACCCTGCCA TCTAAGGCAC CTTCCACATT GGACTTCACG 
GAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172325.1
CDS	2226..7274
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_017316836.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316836.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGGGCGTAA TCAGCCTTCC CCTCAAGCCG GACTTGCAGG CCCAGGACCC 
GCTGGATACG GTGCGGCTAG ACCGACAATT CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT 
CAGAGCCAGG CTCGGGTATG TGCTGGAAGG CCGACTCCTG ACAGCCAAAG CAAGCCCTCG 
GAGACATTGA AAGGTCCTGC TGTCCTTCCA GAACCACTGA AGCCTCAGGC TGCATTTCCT 
CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA AAGTTACAGG ACCCGCTTGT CCCCATAGCA 
GCACCTACTA TGCCTCCCTC TGGCCCCCAA CCAAATTTGG AAACCCCCCC ACAGCCCCCA 
CCTCGGAGCA GGTCATCTCA AAGCTTGCCT TCAGACTCCT CACCACAGCT GCAGGTAAAA 
ATAAATGGCA TTTCTGGTGT CAAACAAGAG CCAACATTGA AGAGTGACCC ATTTGAAGAT 
CTCTCACTTA GTGTGCTTGC TGTATCAAAG GCTCAGCCTT CTGTTCAGAT TTCACCTGTT 
CTAACCCCAG ACCCAAAGAT GTTGATTCAG TTGCCTTCTG CATCGCAAAG TCAAGTTAAC 
CCTTTGAGTT CTGTAAGTTG CATGCCAACC AGGCCTCCAG GTCCAGAGGA AAGCAAGTCA 
CAGGAGAGTA TGGGCAGTTC TGCAAACCCG TTTCCCAGCC TGCCCTGCCG GAACCCTTTT 
ACAGACAGGA CTGCTGCCCC TGGAAACCCA TTTAGAGTTC AGTCCCAAGA ATCAGAGGCA 
ACTTCTTGGC TCTCCAAAGA AGAGCCTGTT CCCAACAGTC CCTTCCCTCC TCTCATGCCT 
CTCAGTCATG ACACGAGCAA GGCTTCAAGT TCGCTTGGTG GCTTTGAGGA CAATTTTGAT 
CTGCAGAGCC AGTCTACAGT AAAAACAAGC AACCCCAAAG GATGGGTAAC CTTTGACGAA 
GACGACAACT TTCCTACAAC AGGAAAGTCA AAGTCAGTTT GTCCAGACTT AGTGGGTAAC 
GCGCCAGCTT CATTTGATGA TGACTGGAGT AAAGGTGCGA GCGTTTCTTT CTGTGTGTTG 
CCAGCCAGAA GGCCACCACC ACCACCACCT CCTGTCCCTC TGCTACCACC TGGCACAACC 
TCATCTGCAG GACCTAGCAC AACCCTGCCA TCTAAGGCAC CTTCCACATT GGACTTCACG 
GAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_017316836.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172325.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X8, mRNA.

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CloneID	OMu79239
Clone ID Related Accession (Same CDS sequence)	XM_017316836.2 , XM_017316836.1
Accession Version	XM_017316836.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGGGCGTAA TCAGCCTTCC CCTCAAGCCG GACTTGCAGG CCCAGGACCC 
GCTGGATACG GTGCGGCTAG ACCGACAATT CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT 
CAGAGCCAGG CTCGGGTATG TGCTGGAAGG CCGACTCCTG ACAGCCAAAG CAAGCCCTCG 
GAGACATTGA AAGGTCCTGC TGTCCTTCCA GAACCACTGA AGCCTCAGGC TGCATTTCCT 
CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA AAGTTACAGG ACCCGCTTGT CCCCATAGCA 
GCACCTACTA TGCCTCCCTC TGGCCCCCAA CCAAATTTGG AAACCCCCCC ACAGCCCCCA 
CCTCGGAGCA GGTCATCTCA AAGCTTGCCT TCAGACTCCT CACCACAGCT GCAGGTAAAA 
ATAAATGGCA TTTCTGGTGT CAAACAAGAG CCAACATTGA AGAGTGACCC ATTTGAAGAT 
CTCTCACTTA GTGTGCTTGC TGTATCAAAG GCTCAGCCTT CTGTTCAGAT TTCACCTGTT 
CTAACCCCAG ACCCAAAGAT GTTGATTCAG TTGCCTTCTG CATCGCAAAG TCAAGTTAAC 
CCTTTGAGTT CTGTAAGTTG CATGCCAACC AGGCCTCCAG GTCCAGAGGA AAGCAAGTCA 
CAGGAGAGTA TGGGCAGTTC TGCAAACCCG TTTCCCAGCC TGCCCTGCCG GAACCCTTTT 
ACAGACAGGA CTGCTGCCCC TGGAAACCCA TTTAGAGTTC AGTCCCAAGA ATCAGAGGCA 
ACTTCTTGGC TCTCCAAAGA AGAGCCTGTT CCCAACAGTC CCTTCCCTCC TCTCATGCCT 
CTCAGTCATG ACACGAGCAA GGCTTCAAGT TCGCTTGGTG GCTTTGAGGA CAATTTTGAT 
CTGCAGAGCC AGTCTACAGT AAAAACAAGC AACCCCAAAG GATGGGTAAC CTTTGACGAA 
GACGACAACT TTCCTACAAC AGGAAAGTCA AAGTCAGTTT GTCCAGACTT AGTGGGTAAC 
GCGCCAGCTT CATTTGATGA TGACTGGAGT AAAGGTGCGA GCGTTTCTTT CTGTGTGTTG 
CCAGCCAGAA GGCCACCACC ACCACCACCT CCTGTCCCTC TGCTACCACC TGGCACAACC 
TCATCTGCAG GACCTAGCAC AACCCTGCCA TCTAAGGCAC CTTCCACATT GGACTTCACG 
GAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172325.1
CDS	388..5436
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_017316836.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316836.1

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGGGCGTAA TCAGCCTTCC CCTCAAGCCG GACTTGCAGG CCCAGGACCC 
GCTGGATACG GTGCGGCTAG ACCGACAATT CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT 
CAGAGCCAGG CTCGGGTATG TGCTGGAAGG CCGACTCCTG ACAGCCAAAG CAAGCCCTCG 
GAGACATTGA AAGGTCCTGC TGTCCTTCCA GAACCACTGA AGCCTCAGGC TGCATTTCCT 
CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA AAGTTACAGG ACCCGCTTGT CCCCATAGCA 
GCACCTACTA TGCCTCCCTC TGGCCCCCAA CCAAATTTGG AAACCCCCCC ACAGCCCCCA 
CCTCGGAGCA GGTCATCTCA AAGCTTGCCT TCAGACTCCT CACCACAGCT GCAGGTAAAA 
ATAAATGGCA TTTCTGGTGT CAAACAAGAG CCAACATTGA AGAGTGACCC ATTTGAAGAT 
CTCTCACTTA GTGTGCTTGC TGTATCAAAG GCTCAGCCTT CTGTTCAGAT TTCACCTGTT 
CTAACCCCAG ACCCAAAGAT GTTGATTCAG TTGCCTTCTG CATCGCAAAG TCAAGTTAAC 
CCTTTGAGTT CTGTAAGTTG CATGCCAACC AGGCCTCCAG GTCCAGAGGA AAGCAAGTCA 
CAGGAGAGTA TGGGCAGTTC TGCAAACCCG TTTCCCAGCC TGCCCTGCCG GAACCCTTTT 
ACAGACAGGA CTGCTGCCCC TGGAAACCCA TTTAGAGTTC AGTCCCAAGA ATCAGAGGCA 
ACTTCTTGGC TCTCCAAAGA AGAGCCTGTT CCCAACAGTC CCTTCCCTCC TCTCATGCCT 
CTCAGTCATG ACACGAGCAA GGCTTCAAGT TCGCTTGGTG GCTTTGAGGA CAATTTTGAT 
CTGCAGAGCC AGTCTACAGT AAAAACAAGC AACCCCAAAG GATGGGTAAC CTTTGACGAA 
GACGACAACT TTCCTACAAC AGGAAAGTCA AAGTCAGTTT GTCCAGACTT AGTGGGTAAC 
GCGCCAGCTT CATTTGATGA TGACTGGAGT AAAGGTGCGA GCGTTTCTTT CTGTGTGTTG 
CCAGCCAGAA GGCCACCACC ACCACCACCT CCTGTCCCTC TGCTACCACC TGGCACAACC 
TCATCTGCAG GACCTAGCAC AACCCTGCCA TCTAAGGCAC CTTCCACATT GGACTTCACG 
GAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_030248902.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104762.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X3, mRNA.

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CloneID	OMu86845
Clone ID Related Accession (Same CDS sequence)	XM_030248902.1
Accession Version	XM_030248902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGCATCTTCC AAAGTCTTAA AGAACATGTG TGAGAACTTC 
TACAAATACT CAAAGCCCAA GAAGATCCGA GTGTGTGTTG GCACCTGGAA TGTGAACGGC 
GGGAAGCAGT TCCGCAGCAT AGCGTTCAAG AACCAGACGC TCACAGACTG GCTTCTGGAC 
GCTCCCAAGT TAGCCGGCAT CCAGGAGTTT CAAGATAAGA GAAGTAAGCC AACTGATATA 
TTTGCAATTG GCTTTGAAGA AATGGTGGAG CTGAATGCTG GGAACATTGT GAATGCAAGC 
ACAACCAACC AGAAGCTGTG GGCAGTGGAG CTGCAGAAGA CCATCTCCAG GGACAACAAG 
TATGTCCTGC TGGCCTCGGA GCAGCTGGTG GGTGTCTGCC TGTTTGTCTT TATCAGACCA 
CAGCACGCTC CTTTTATCAG GGATGTTGCA GTTGATACTG TGAAAACTGG CATGGGAGGT 
GCAACTGGAA ACAAGGGCGC CGTTGCAATT CGGATGCTCT TCCACACCAC CAGCCTTTGC 
TTCGTCTGCA GCCACTTTGC TGCAGGGCAG TCCCAAGTCA AAGAGAGAAA TGAAGATTTT 
GTAGAAATAG CACGAAAACT GAGTTTCCCC ATGGGACGAA TGCTCTTCTC CCATGACTAT 
GTATTTTGGT GTGGTGATTT CAACTATCGA ATTGACCTTC CGAATGAAGA GGTTAAAGAG 
CTCATAAGAC AACAAAACTG GGATTCTCTG ATCGCTGGGG ATCAGCTCAT CAACCAGAAA 
AATGCAGGAC AGATCTTTAG AGGATTCTTA GAGGGAAAAG TAACGTTTGC TCCAACCTAT 
AAATATGACT TGTTTTCTGA AGACTATGAC ACCAGTGAGA AGTGTCGCAC CCCTGCTTGG 
ACAGACCGTG TCCTCTGGAG AAGGAGGAAG TGGCCTTTTG ACAGATCAGC TGAAGATTTA 
GATCTCCTGA ATGCTAGTTT CCAAGATGAA AGTAAAATTC TTTATACATG GACCCCTGGC 
ACCTTGCTGC ACTATGGAAG AGCCGAGCTG AAGACTTCTG ACCATAGGCC CGTCGTTGCC 
TTGATTGACA TAGATATATT TGAAGTTGAA GCTGAAGAGA GACAAAAAAT TTATAAAGAA 
GTTATTGCCG TCCAGGGCCC ACCAGATGGC ACAGTGCTGG TCTCAATCAA AAGTTCTGCA 
CAAGAAAGTA CTTTTTTTGA TGATGCTTTG ATTGATGAGC TTCTGCGGCA GTTTGCACAC 
TTTGGTGAAG TTATACTCAT AAGATTTGTA GAAGATAAAA TGTGGGTTAC ATTTTTGGAG 
GGAAGCTCTG CCTTGAATGC TCTGAGCCTA AATGGGAAAG AGCTATTAAA TCGGACTATA 
ACAATTACTT TAAAAAGTCC AGACTGGATC AAACATTTGG AAGAAGAGAT GAGTTTAGAG 
AAAATCAGTG TCACGTTGCC CTCATCAGCA AGCTCCACCC TGCTTGGTGA AGACGCAGAG 
GTCGCAGCAG ACTTTGACAT GGAAGGTGAC GTCGATGACT ACAGCGCTGA AGTAGAGGAG 
CTTCTTCCTC AGCATCTGCA GCCGTCCTCA AGTTCTGGCC TGGGCACTTC TCCAAGTTCT 
TCACCCCGGA CCAGTCCCTG CCAGTCCCCC ACAGTACCAG AGTACTCCGC CCCTTCTCTT 
CCCATCAGAC CTAGCCGAGC ACCGTCAAGA ACTCCAGGAC CTCCAAGTTC GCAGGGCTCT 
CCAGTTGACA CTCAGCCAGC GGCCCAGAAG GATTCTTCCC AGACTCTAGA GCCCAAGAGA 
CCACCCCCTC CCCGCCCAGT CGCTCCTCCG GCACGTCCTG CCCCACCACA GAGACCACCT 
CCACCTTCAG GGGCTAGGAG TCCTGCGCCT GCTAGAAAAG AATTTGGAGG TGTTGGAGCC 
CCTCCCAGTC CCGGGGTTGC TAGGAGAGAG ATAGAAGCAC CCAAAAGCCC TGGAACAGCA 
CGGAAAGATA ATATAGGGCG TAATCAGCCT TCCCCTCAAG CCGGACTTGC AGGCCCAGGA 
CCCGCTGGAT ACGGTGCGGC TAGACCGACA ATTCCAGCTC GTGCTGGAGT GATCAGCGCC 
CCTCAGAGCC AGGCTCGGGT ATGTGCTGGA AGGCCGACTC CTGACAGCCA AAGCAAGCCC 
TCGGAGACAT TGAAAGGTCC TGCTGTCCTT CCAGAACCAC TGAAGCCTCA GGCTGCATTT 
CCTCAGCAGC CTTCTCTGCC CACACCTGCT CAAAAGTTAC AGGACCCGCT TGTCCCCATA 
GCAGCACCTA CTATGCCTCC CTCTGGCCCC CAACCAAATT TGGAAACCCC CCCACAGCCC 
CCACCTCGGA GCAGGTCATC TCAAAGCTTG CCTTCAGACT CCTCACCACA GCTGCAGGTA 
AAAATAAATG GCATTTCTGG TGTCAAACAA GAGCCAACAT TGAAGAGTGA CCCATTTGAA 
GATCTCTCAC TTAGTGTGCT TGCTGTATCA AAGGCTCAGC CTTCTGTTCA GATTTCACCT 
GTTCTAACCC CAGACCCAAA GATGTTGATT CAGTTGCCTT CTGCATCGCA AAGTCAAGTT 
AACCCTTTGA GTTCTGTAAG TTGCATGCCA ACCAGGCCTC CAGGTCCAGA GGAAAGCAAG 
TCACAGGAGA GTATGGGCAG TTCTGCAAAC CCGTTTCCCA GCCTGCCCTG CCGGAACCCT 
TTTACAGACA GGACTGCTGC CCCTGGAAAC CCATTTAGAG TTCAGTCCCA AGAATCAGAG 
GCAACTTCTT GGCTCTCCAA AGAAGAGCCT GTTCCCAACA GTCCCTTCCC TCCTCTCATG 
CCTCTCAGTC ATGACACGAG CAAGGCTTCA AGTTCGCTTG GTGGCTTTGA GGACAATTTT 
GATCTGCAGA GCCAGTCTAC AGTAAAAACA AGCAACCCCA AAGGATGGGT AACCTTTGAC 
GAAGACGACA ACTTTCCTAC AACAGGAAAG TCAAAGTCAG TTTGTCCAGA CTTAGTGGGT 
AACGCGCCAG CTTCATTTGA TGATGACTGG AGTAAAGGTG CGAGCGTTTC TTTCTGTGTG 
TTGCCAGCCA GAAGGCCACC ACCACCACCA CCTCCTGTCC CTCTGCTACC ACCTGGCACA 
ACCTCATCTG CAGGACCTAG CACAACCCTG CCATCTAAGG CACCTTCCAC ATTGGACTTC 
ACGGAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104762.1
CDS	2226..7337
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_030248902.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248902.1

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGCATCTTCC AAAGTCTTAA AGAACATGTG TGAGAACTTC 
TACAAATACT CAAAGCCCAA GAAGATCCGA GTGTGTGTTG GCACCTGGAA TGTGAACGGC 
GGGAAGCAGT TCCGCAGCAT AGCGTTCAAG AACCAGACGC TCACAGACTG GCTTCTGGAC 
GCTCCCAAGT TAGCCGGCAT CCAGGAGTTT CAAGATAAGA GAAGTAAGCC AACTGATATA 
TTTGCAATTG GCTTTGAAGA AATGGTGGAG CTGAATGCTG GGAACATTGT GAATGCAAGC 
ACAACCAACC AGAAGCTGTG GGCAGTGGAG CTGCAGAAGA CCATCTCCAG GGACAACAAG 
TATGTCCTGC TGGCCTCGGA GCAGCTGGTG GGTGTCTGCC TGTTTGTCTT TATCAGACCA 
CAGCACGCTC CTTTTATCAG GGATGTTGCA GTTGATACTG TGAAAACTGG CATGGGAGGT 
GCAACTGGAA ACAAGGGCGC CGTTGCAATT CGGATGCTCT TCCACACCAC CAGCCTTTGC 
TTCGTCTGCA GCCACTTTGC TGCAGGGCAG TCCCAAGTCA AAGAGAGAAA TGAAGATTTT 
GTAGAAATAG CACGAAAACT GAGTTTCCCC ATGGGACGAA TGCTCTTCTC CCATGACTAT 
GTATTTTGGT GTGGTGATTT CAACTATCGA ATTGACCTTC CGAATGAAGA GGTTAAAGAG 
CTCATAAGAC AACAAAACTG GGATTCTCTG ATCGCTGGGG ATCAGCTCAT CAACCAGAAA 
AATGCAGGAC AGATCTTTAG AGGATTCTTA GAGGGAAAAG TAACGTTTGC TCCAACCTAT 
AAATATGACT TGTTTTCTGA AGACTATGAC ACCAGTGAGA AGTGTCGCAC CCCTGCTTGG 
ACAGACCGTG TCCTCTGGAG AAGGAGGAAG TGGCCTTTTG ACAGATCAGC TGAAGATTTA 
GATCTCCTGA ATGCTAGTTT CCAAGATGAA AGTAAAATTC TTTATACATG GACCCCTGGC 
ACCTTGCTGC ACTATGGAAG AGCCGAGCTG AAGACTTCTG ACCATAGGCC CGTCGTTGCC 
TTGATTGACA TAGATATATT TGAAGTTGAA GCTGAAGAGA GACAAAAAAT TTATAAAGAA 
GTTATTGCCG TCCAGGGCCC ACCAGATGGC ACAGTGCTGG TCTCAATCAA AAGTTCTGCA 
CAAGAAAGTA CTTTTTTTGA TGATGCTTTG ATTGATGAGC TTCTGCGGCA GTTTGCACAC 
TTTGGTGAAG TTATACTCAT AAGATTTGTA GAAGATAAAA TGTGGGTTAC ATTTTTGGAG 
GGAAGCTCTG CCTTGAATGC TCTGAGCCTA AATGGGAAAG AGCTATTAAA TCGGACTATA 
ACAATTACTT TAAAAAGTCC AGACTGGATC AAACATTTGG AAGAAGAGAT GAGTTTAGAG 
AAAATCAGTG TCACGTTGCC CTCATCAGCA AGCTCCACCC TGCTTGGTGA AGACGCAGAG 
GTCGCAGCAG ACTTTGACAT GGAAGGTGAC GTCGATGACT ACAGCGCTGA AGTAGAGGAG 
CTTCTTCCTC AGCATCTGCA GCCGTCCTCA AGTTCTGGCC TGGGCACTTC TCCAAGTTCT 
TCACCCCGGA CCAGTCCCTG CCAGTCCCCC ACAGTACCAG AGTACTCCGC CCCTTCTCTT 
CCCATCAGAC CTAGCCGAGC ACCGTCAAGA ACTCCAGGAC CTCCAAGTTC GCAGGGCTCT 
CCAGTTGACA CTCAGCCAGC GGCCCAGAAG GATTCTTCCC AGACTCTAGA GCCCAAGAGA 
CCACCCCCTC CCCGCCCAGT CGCTCCTCCG GCACGTCCTG CCCCACCACA GAGACCACCT 
CCACCTTCAG GGGCTAGGAG TCCTGCGCCT GCTAGAAAAG AATTTGGAGG TGTTGGAGCC 
CCTCCCAGTC CCGGGGTTGC TAGGAGAGAG ATAGAAGCAC CCAAAAGCCC TGGAACAGCA 
CGGAAAGATA ATATAGGGCG TAATCAGCCT TCCCCTCAAG CCGGACTTGC AGGCCCAGGA 
CCCGCTGGAT ACGGTGCGGC TAGACCGACA ATTCCAGCTC GTGCTGGAGT GATCAGCGCC 
CCTCAGAGCC AGGCTCGGGT ATGTGCTGGA AGGCCGACTC CTGACAGCCA AAGCAAGCCC 
TCGGAGACAT TGAAAGGTCC TGCTGTCCTT CCAGAACCAC TGAAGCCTCA GGCTGCATTT 
CCTCAGCAGC CTTCTCTGCC CACACCTGCT CAAAAGTTAC AGGACCCGCT TGTCCCCATA 
GCAGCACCTA CTATGCCTCC CTCTGGCCCC CAACCAAATT TGGAAACCCC CCCACAGCCC 
CCACCTCGGA GCAGGTCATC TCAAAGCTTG CCTTCAGACT CCTCACCACA GCTGCAGGTA 
AAAATAAATG GCATTTCTGG TGTCAAACAA GAGCCAACAT TGAAGAGTGA CCCATTTGAA 
GATCTCTCAC TTAGTGTGCT TGCTGTATCA AAGGCTCAGC CTTCTGTTCA GATTTCACCT 
GTTCTAACCC CAGACCCAAA GATGTTGATT CAGTTGCCTT CTGCATCGCA AAGTCAAGTT 
AACCCTTTGA GTTCTGTAAG TTGCATGCCA ACCAGGCCTC CAGGTCCAGA GGAAAGCAAG 
TCACAGGAGA GTATGGGCAG TTCTGCAAAC CCGTTTCCCA GCCTGCCCTG CCGGAACCCT 
TTTACAGACA GGACTGCTGC CCCTGGAAAC CCATTTAGAG TTCAGTCCCA AGAATCAGAG 
GCAACTTCTT GGCTCTCCAA AGAAGAGCCT GTTCCCAACA GTCCCTTCCC TCCTCTCATG 
CCTCTCAGTC ATGACACGAG CAAGGCTTCA AGTTCGCTTG GTGGCTTTGA GGACAATTTT 
GATCTGCAGA GCCAGTCTAC AGTAAAAACA AGCAACCCCA AAGGATGGGT AACCTTTGAC 
GAAGACGACA ACTTTCCTAC AACAGGAAAG TCAAAGTCAG TTTGTCCAGA CTTAGTGGGT 
AACGCGCCAG CTTCATTTGA TGATGACTGG AGTAAAGGTG CGAGCGTTTC TTTCTGTGTG 
TTGCCAGCCA GAAGGCCACC ACCACCACCA CCTCCTGTCC CTCTGCTACC ACCTGGCACA 
ACCTCATCTG CAGGACCTAG CACAACCCTG CCATCTAAGG CACCTTCCAC ATTGGACTTC 
ACGGAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_030248903.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104763.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X9, mRNA.

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CloneID	OMu86846
Clone ID Related Accession (Same CDS sequence)	XM_030248903.1
Accession Version	XM_030248903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGCACCCA AAAGCCCTGG AACAGCACGG 
AAAGATAATA TAGGGCGTAA TCAGCCTTCC CCTCAAGCCG GACTTGCAGG CCCAGGACCC 
GCTGGATACG GTGCGGCTAG ACCGACAATT CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT 
CAGAGCCAGG CTCGGGTATG TGCTGGAAGG CCGACTCCTG ACAGCCAAAG CAAGCCCTCG 
GAGACATTGA AAGGTCCTGC TGTCCTTCCA GAACCACTGA AGCCTCAGGC TGCATTTCCT 
CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA AAGTTACAGG ACCCGCTTGT CCCCATAGCA 
GCACCTACTA TGCCTCCCTC TGGCCCCCAA CCAAATTTGG AAACCCCCCC ACAGCCCCCA 
CCTCGGAGCA GGTCATCTCA AAGCTTGCCT TCAGACTCCT CACCACAGCT GCAGGTAAAA 
ATAAATGGCA TTTCTGGTGT CAAACAAGAG CCAACATTGA AGAGTGACCC ATTTGAAGAT 
CTCTCACTTA GTGTGCTTGC TGTATCAAAG GCTCAGCCTT CTGTTCAGAT TTCACCTGTT 
CTAACCCCAG ACCCAAAGAT GTTGATTCAG TTGCCTTCTG CATCGCAAAG TCAAGTTAAC 
CCTTTGAGTT CTGTAAGTTG CATGCCAACC AGGCCTCCAG GTCCAGAGGA AAGCAAGTCA 
CAGGAGAGTA TGGGCAGTTC TGCAAACCCG TTTCCCAGCC TGCCCTGCCG GAACCCTTTT 
ACAGACAGGA CTGCTGCCCC TGGAAACCCA TTTAGAGTTC AGTCCCAAGA ATCAGAGGCA 
ACTTCTTGGC TCTCCAAAGA AGAGCCTGTT CCCAACAGTC CCTTCCCTCC TCTCATGCCT 
CTCAGTCATG ACACGAGCAA GGCTTCAAGT TCGCTTGGTG GCTTTGAGGA CAATTTTGAT 
CTGCAGAGCC AGTCTACAGT AAAAACAAGC AACCCCAAAG GATGGGTAAC CTTTGACGAA 
GACGACAACT TTCCTACAAC AGGAAAGTCA AAGTCAGTTT GTCCAGACTT AGTGGGTAAC 
GCGCCAGCTT CATTTGATGA TGACTGGAGT AAAGGTGCGA GCGTTTCTTT CTGTGTGTTG 
CCAGCCAGAA GGCCACCACC ACCACCACCT CCTGTCCCTC TGCTACCACC TGGCACAACC 
TCATCTGCAG GACCTAGCAC AACCCTGCCA TCTAAGGCAC CTTCCACATT GGACTTCACG 
GAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104763.1
CDS	2226..7274
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_030248903.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248903.1

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGCACCCA AAAGCCCTGG AACAGCACGG 
AAAGATAATA TAGGGCGTAA TCAGCCTTCC CCTCAAGCCG GACTTGCAGG CCCAGGACCC 
GCTGGATACG GTGCGGCTAG ACCGACAATT CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT 
CAGAGCCAGG CTCGGGTATG TGCTGGAAGG CCGACTCCTG ACAGCCAAAG CAAGCCCTCG 
GAGACATTGA AAGGTCCTGC TGTCCTTCCA GAACCACTGA AGCCTCAGGC TGCATTTCCT 
CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA AAGTTACAGG ACCCGCTTGT CCCCATAGCA 
GCACCTACTA TGCCTCCCTC TGGCCCCCAA CCAAATTTGG AAACCCCCCC ACAGCCCCCA 
CCTCGGAGCA GGTCATCTCA AAGCTTGCCT TCAGACTCCT CACCACAGCT GCAGGTAAAA 
ATAAATGGCA TTTCTGGTGT CAAACAAGAG CCAACATTGA AGAGTGACCC ATTTGAAGAT 
CTCTCACTTA GTGTGCTTGC TGTATCAAAG GCTCAGCCTT CTGTTCAGAT TTCACCTGTT 
CTAACCCCAG ACCCAAAGAT GTTGATTCAG TTGCCTTCTG CATCGCAAAG TCAAGTTAAC 
CCTTTGAGTT CTGTAAGTTG CATGCCAACC AGGCCTCCAG GTCCAGAGGA AAGCAAGTCA 
CAGGAGAGTA TGGGCAGTTC TGCAAACCCG TTTCCCAGCC TGCCCTGCCG GAACCCTTTT 
ACAGACAGGA CTGCTGCCCC TGGAAACCCA TTTAGAGTTC AGTCCCAAGA ATCAGAGGCA 
ACTTCTTGGC TCTCCAAAGA AGAGCCTGTT CCCAACAGTC CCTTCCCTCC TCTCATGCCT 
CTCAGTCATG ACACGAGCAA GGCTTCAAGT TCGCTTGGTG GCTTTGAGGA CAATTTTGAT 
CTGCAGAGCC AGTCTACAGT AAAAACAAGC AACCCCAAAG GATGGGTAAC CTTTGACGAA 
GACGACAACT TTCCTACAAC AGGAAAGTCA AAGTCAGTTT GTCCAGACTT AGTGGGTAAC 
GCGCCAGCTT CATTTGATGA TGACTGGAGT AAAGGTGCGA GCGTTTCTTT CTGTGTGTTG 
CCAGCCAGAA GGCCACCACC ACCACCACCT CCTGTCCCTC TGCTACCACC TGGCACAACC 
TCATCTGCAG GACCTAGCAC AACCCTGCCA TCTAAGGCAC CTTCCACATT GGACTTCACG 
GAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_030248904.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104764.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X13, mRNA.

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CloneID	OMu86847
Clone ID Related Accession (Same CDS sequence)	XM_030248904.1
Accession Version	XM_030248904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGCATCTTCC AAAGTCTTAA AGAACATGTG TGAGAACTTC 
TACAAATACT CAAAGCCCAA GAAGATCCGA GTGTGTGTTG GCACCTGGAA TGTGAACGGC 
GGGAAGCAGT TCCGCAGCAT AGCGTTCAAG AACCAGACGC TCACAGACTG GCTTCTGGAC 
GCTCCCAAGT TAGCCGGCAT CCAGGAGTTT CAAGATAAGA GAAGTAAGCC AACTGATATA 
TTTGCAATTG GCTTTGAAGA AATGGTGGAG CTGAATGCTG GGAACATTGT GAATGCAAGC 
ACAACCAACC AGAAGCTGTG GGCAGTGGAG CTGCAGAAGA CCATCTCCAG GGACAACAAG 
TATGTCCTGC TGGCCTCGGA GCAGCTGGTG GGTGTCTGCC TGTTTGTCTT TATCAGACCA 
CAGCACGCTC CTTTTATCAG GGATGTTGCA GTTGATACTG TGAAAACTGG CATGGGAGGT 
GCAACTGGAA ACAAGGGCGC CGTTGCAATT CGGATGCTCT TCCACACCAC CAGCCTTTGC 
TTCGTCTGCA GCCACTTTGC TGCAGGGCAG TCCCAAGTCA AAGAGAGAAA TGAAGATTTT 
GTAGAAATAG CACGAAAACT GAGTTTCCCC ATGGGACGAA TGCTCTTCTC CCATGACTAT 
GTATTTTGGT GTGGTGATTT CAACTATCGA ATTGACCTTC CGAATGAAGA GGTTAAAGAG 
CTCATAAGAC AACAAAACTG GGATTCTCTG ATCGCTGGGG ATCAGCTCAT CAACCAGAAA 
AATGCAGGAC AGATCTTTAG AGGATTCTTA GAGGGAAAAG TAACGTTTGC TCCAACCTAT 
AAATATGACT TGTTTTCTGA AGACTATGAC ACCAGTGAGA AGTGTCGCAC CCCTGCTTGG 
ACAGACCGTG TCCTCTGGAG AAGGAGGAAG TGGCCTTTTG ACAGATCAGC TGAAGATTTA 
GATCTCCTGA ATGCTAGTTT CCAAGATGAA AGTAAAATTC TTTATACATG GACCCCTGGC 
ACCTTGCTGC ACTATGGAAG AGCCGAGCTG AAGACTTCTG ACCATAGGCC CGTCGTTGCC 
TTGATTGACA TAGATATATT TGAAGTTGAA GCTGAAGAGA GACAAAAAAT TTATAAAGAA 
GTTATTGCCG TCCAGGGCCC ACCAGATGGC ACAGTGCTGG TCTCAATCAA AAGTTCTGCA 
CAAGAAAGTA CTTTTTTTGA TGATGCTTTG ATTGATGAGC TTCTGCGGCA GTTTGCACAC 
TTTGGTGAAG TTATACTCAT AAGATTTGTA GAAGATAAAA TGTGGGTTAC ATTTTTGGAG 
GGAAGCTCTG CCTTGAATGC TCTGAGCCTA AATGGGAAAG AGCTATTAAA TCGGACTATA 
ACAATTACTT TAAAAAGTCC AGACTGGATC AAACATTTGG AAGAAGAGAT GAGTTTAGAG 
AAAATCAGTG TCACGTTGCC CTCATCAGCA AGCTCCACCC TGCTTGGTGA AGACGCAGAG 
GTCGCAGCAG ACTTTGACAT GGAAGGTGAC GTCGATGACT ACAGCGCTGA AGTAGAGGAG 
CTTCTTCCTC AGCATCTGCA GCCGTCCTCA AGTTCTGGCC TGGGCACTTC TCCAAGTTCT 
TCACCCCGGA CCAGTCCCTG CCAGTCCCCC ACAGTACCAG AGTACTCCGC CCCTTCTCTT 
CCCATCAGAC CTAGCCGAGC ACCGTCAAGA ACTCCAGGAC CTCCAAGTTC GCAGGGCTCT 
CCAGTTGACA CTCAGCCAGC GGCCCAGAAG GATTCTTCCC AGACTCTAGA GCCCAAGAGA 
CCACCCCCTC CCCGCCCAGT CGCTCCTCCG GCACGTCCTG CCCCACCACA GAGACCACCT 
CCACCTTCAG GGCGTAATCA GCCTTCCCCT CAAGCCGGAC TTGCAGGCCC AGGACCCGCT 
GGATACGGTG CGGCTAGACC GACAATTCCA GCTCGTGCTG GAGTGATCAG CGCCCCTCAG 
AGCCAGGCTC GGGTATGTGC TGGAAGGCCG ACTCCTGACA GCCAAAGCAA GCCCTCGGAG 
ACATTGAAAG GTCCTGCTGT CCTTCCAGAA CCACTGAAGC CTCAGGCTGC ATTTCCTCAG 
CAGCCTTCTC TGCCCACACC TGCTCAAAAG TTACAGGACC CGCTTGTCCC CATAGCAGCA 
CCTACTATGC CTCCCTCTGG CCCCCAACCA AATTTGGAAA CCCCCCCACA GCCCCCACCT 
CGGAGCAGGT CATCTCAAAG CTTGCCTTCA GACTCCTCAC CACAGCTGCA GGTAAAAATA 
AATGGCATTT CTGGTGTCAA ACAAGAGCCA ACATTGAAGA GTGACCCATT TGAAGATCTC 
TCACTTAGTG TGCTTGCTGT ATCAAAGGCT CAGCCTTCTG TTCAGATTTC ACCTGTTCTA 
ACCCCAGACC CAAAGATGTT GATTCAGTTG CCTTCTGCAT CGCAAAGTCA AGTTAACCCT 
TTGAGTTCTG TAAGTTGCAT GCCAACCAGG CCTCCAGGTC CAGAGGAAAG CAAGTCACAG 
GAGAGTATGG GCAGTTCTGC AAACCCGTTT CCCAGCCTGC CCTGCCGGAA CCCTTTTACA 
GACAGGACTG CTGCCCCTGG AAACCCATTT AGAGTTCAGT CCCAAGAATC AGAGGCAACT 
TCTTGGCTCT CCAAAGAAGA GCCTGTTCCC AACAGTCCCT TCCCTCCTCT CATGCCTCTC 
AGTCATGACA CGAGCAAGGC TTCAAGTTCG CTTGGTGGCT TTGAGGACAA TTTTGATCTG 
CAGAGCCAGT CTACAGTAAA AACAAGCAAC CCCAAAGGAT GGGTAACCTT TGACGAAGAC 
GACAACTTTC CTACAACAGG AAAGTCAAAG TCAGTTTGTC CAGACTTAGT GGGTAACGCG 
CCAGCTTCAT TTGATGATGA CTGGAGTAAA GGTGCGAGCG TTTCTTTCTG TGTGTTGCCA 
GCCAGAAGGC CACCACCACC ACCACCTCCT GTCCCTCTGC TACCACCTGG CACAACCTCA 
TCTGCAGGAC CTAGCACAAC CCTGCCATCT AAGGCACCTT CCACATTGGA CTTCACGGAA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104764.1
CDS	2226..7211
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_030248904.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248904.1

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGCATCTTCC AAAGTCTTAA AGAACATGTG TGAGAACTTC 
TACAAATACT CAAAGCCCAA GAAGATCCGA GTGTGTGTTG GCACCTGGAA TGTGAACGGC 
GGGAAGCAGT TCCGCAGCAT AGCGTTCAAG AACCAGACGC TCACAGACTG GCTTCTGGAC 
GCTCCCAAGT TAGCCGGCAT CCAGGAGTTT CAAGATAAGA GAAGTAAGCC AACTGATATA 
TTTGCAATTG GCTTTGAAGA AATGGTGGAG CTGAATGCTG GGAACATTGT GAATGCAAGC 
ACAACCAACC AGAAGCTGTG GGCAGTGGAG CTGCAGAAGA CCATCTCCAG GGACAACAAG 
TATGTCCTGC TGGCCTCGGA GCAGCTGGTG GGTGTCTGCC TGTTTGTCTT TATCAGACCA 
CAGCACGCTC CTTTTATCAG GGATGTTGCA GTTGATACTG TGAAAACTGG CATGGGAGGT 
GCAACTGGAA ACAAGGGCGC CGTTGCAATT CGGATGCTCT TCCACACCAC CAGCCTTTGC 
TTCGTCTGCA GCCACTTTGC TGCAGGGCAG TCCCAAGTCA AAGAGAGAAA TGAAGATTTT 
GTAGAAATAG CACGAAAACT GAGTTTCCCC ATGGGACGAA TGCTCTTCTC CCATGACTAT 
GTATTTTGGT GTGGTGATTT CAACTATCGA ATTGACCTTC CGAATGAAGA GGTTAAAGAG 
CTCATAAGAC AACAAAACTG GGATTCTCTG ATCGCTGGGG ATCAGCTCAT CAACCAGAAA 
AATGCAGGAC AGATCTTTAG AGGATTCTTA GAGGGAAAAG TAACGTTTGC TCCAACCTAT 
AAATATGACT TGTTTTCTGA AGACTATGAC ACCAGTGAGA AGTGTCGCAC CCCTGCTTGG 
ACAGACCGTG TCCTCTGGAG AAGGAGGAAG TGGCCTTTTG ACAGATCAGC TGAAGATTTA 
GATCTCCTGA ATGCTAGTTT CCAAGATGAA AGTAAAATTC TTTATACATG GACCCCTGGC 
ACCTTGCTGC ACTATGGAAG AGCCGAGCTG AAGACTTCTG ACCATAGGCC CGTCGTTGCC 
TTGATTGACA TAGATATATT TGAAGTTGAA GCTGAAGAGA GACAAAAAAT TTATAAAGAA 
GTTATTGCCG TCCAGGGCCC ACCAGATGGC ACAGTGCTGG TCTCAATCAA AAGTTCTGCA 
CAAGAAAGTA CTTTTTTTGA TGATGCTTTG ATTGATGAGC TTCTGCGGCA GTTTGCACAC 
TTTGGTGAAG TTATACTCAT AAGATTTGTA GAAGATAAAA TGTGGGTTAC ATTTTTGGAG 
GGAAGCTCTG CCTTGAATGC TCTGAGCCTA AATGGGAAAG AGCTATTAAA TCGGACTATA 
ACAATTACTT TAAAAAGTCC AGACTGGATC AAACATTTGG AAGAAGAGAT GAGTTTAGAG 
AAAATCAGTG TCACGTTGCC CTCATCAGCA AGCTCCACCC TGCTTGGTGA AGACGCAGAG 
GTCGCAGCAG ACTTTGACAT GGAAGGTGAC GTCGATGACT ACAGCGCTGA AGTAGAGGAG 
CTTCTTCCTC AGCATCTGCA GCCGTCCTCA AGTTCTGGCC TGGGCACTTC TCCAAGTTCT 
TCACCCCGGA CCAGTCCCTG CCAGTCCCCC ACAGTACCAG AGTACTCCGC CCCTTCTCTT 
CCCATCAGAC CTAGCCGAGC ACCGTCAAGA ACTCCAGGAC CTCCAAGTTC GCAGGGCTCT 
CCAGTTGACA CTCAGCCAGC GGCCCAGAAG GATTCTTCCC AGACTCTAGA GCCCAAGAGA 
CCACCCCCTC CCCGCCCAGT CGCTCCTCCG GCACGTCCTG CCCCACCACA GAGACCACCT 
CCACCTTCAG GGCGTAATCA GCCTTCCCCT CAAGCCGGAC TTGCAGGCCC AGGACCCGCT 
GGATACGGTG CGGCTAGACC GACAATTCCA GCTCGTGCTG GAGTGATCAG CGCCCCTCAG 
AGCCAGGCTC GGGTATGTGC TGGAAGGCCG ACTCCTGACA GCCAAAGCAA GCCCTCGGAG 
ACATTGAAAG GTCCTGCTGT CCTTCCAGAA CCACTGAAGC CTCAGGCTGC ATTTCCTCAG 
CAGCCTTCTC TGCCCACACC TGCTCAAAAG TTACAGGACC CGCTTGTCCC CATAGCAGCA 
CCTACTATGC CTCCCTCTGG CCCCCAACCA AATTTGGAAA CCCCCCCACA GCCCCCACCT 
CGGAGCAGGT CATCTCAAAG CTTGCCTTCA GACTCCTCAC CACAGCTGCA GGTAAAAATA 
AATGGCATTT CTGGTGTCAA ACAAGAGCCA ACATTGAAGA GTGACCCATT TGAAGATCTC 
TCACTTAGTG TGCTTGCTGT ATCAAAGGCT CAGCCTTCTG TTCAGATTTC ACCTGTTCTA 
ACCCCAGACC CAAAGATGTT GATTCAGTTG CCTTCTGCAT CGCAAAGTCA AGTTAACCCT 
TTGAGTTCTG TAAGTTGCAT GCCAACCAGG CCTCCAGGTC CAGAGGAAAG CAAGTCACAG 
GAGAGTATGG GCAGTTCTGC AAACCCGTTT CCCAGCCTGC CCTGCCGGAA CCCTTTTACA 
GACAGGACTG CTGCCCCTGG AAACCCATTT AGAGTTCAGT CCCAAGAATC AGAGGCAACT 
TCTTGGCTCT CCAAAGAAGA GCCTGTTCCC AACAGTCCCT TCCCTCCTCT CATGCCTCTC 
AGTCATGACA CGAGCAAGGC TTCAAGTTCG CTTGGTGGCT TTGAGGACAA TTTTGATCTG 
CAGAGCCAGT CTACAGTAAA AACAAGCAAC CCCAAAGGAT GGGTAACCTT TGACGAAGAC 
GACAACTTTC CTACAACAGG AAAGTCAAAG TCAGTTTGTC CAGACTTAGT GGGTAACGCG 
CCAGCTTCAT TTGATGATGA CTGGAGTAAA GGTGCGAGCG TTTCTTTCTG TGTGTTGCCA 
GCCAGAAGGC CACCACCACC ACCACCTCCT GTCCCTCTGC TACCACCTGG CACAACCTCA 
TCTGCAGGAC CTAGCACAAC CCTGCCATCT AAGGCACCTT CCACATTGGA CTTCACGGAA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_030248905.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104765.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X15, mRNA.

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CloneID	OMu86848
Clone ID Related Accession (Same CDS sequence)	XM_030248905.1
Accession Version	XM_030248905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTCA GCAAAGGATT TCGAATCTAC CACAAATTGG ATCCCCCACC GTTCAGCCTC 
ATAGTGGAAA CTAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTGGCTGTG 
CTCTCATCTG CAGAAAAGGA GGCTATTAAA GGCACATATG CCAAAGTACT GGATGCATAC 
GGACTTTTGG GAGTTTTACG ATTAAATCTT GGTGATACCA TGCTGCACTA TCTGGTCCTA 
GTCACTGGAT GCATGTCTGT GGGGAAAATC CAAGAATCTG AAGTTTTCCG AGTTACTTCC 
ACTGAGTTTA TATCATTGCG AGTTGATGCT TCAGATGAGG ACCGCATTTC GGAAGTACGA 
AAAGTTTTGA ACTCGGGAAA CTTTTATTTT GCATGGTCTG CATCTGGAGT CAGCTTAGAT 
CTGAGTCTGA ATGCACATCG AAGCATGCAA GAGCACACAA CCGACAATAG GTTTTTCTGG 
AATCAGTCTC TGCATTTGCA TCTCAAGCAC TATGGTGTGA ACTGCGATGA CTGGTTATTA 
CGGCTCATGT GTGGGGGAGT AGAGATTAGA ACCATTTATG CTGCCCATAA ACAGGCAAAA 
GCTTGCCTCA TCTCAAGACT AAGCTGTGAA CGAGCTGGGA CAAGGTTCAA TGTCCGGGGA 
ACCAACGATG ATGGTCATGT TGCCAACTTT GTAGAGACAG AGCAGGTTAT ATACTTAGAT 
GACTGTGTCT CTTCCTTCAT ACAAATCCGA GGATCTGTTC CATTGTTCTG GGAGCAACCA 
GGGTTGCAAG TGGGATCCCA TCGTGTTCGT ATGTCAAGGG GATTTGAAGC CAATGCGCCT 
GCCTTTGACA GGCATTTCCG AACACTTAAG GACTTATATG GTAAACAGAT AGTAGTGAAT 
CTGCTTGGGT CTAAGGAAGG CGAGCATATG CTGAGCAAGG CTTTCCAGAG TCATTTAAAA 
GCCTCTGAAC ACGCTTCTGA TATCCACATG GTGTCTTTTG ACTATCATCA AATGGTTAAA 
GGAGGAAAGG CAGAAAAACT ACACAGTATT CTCAAACCAC AAGTCCAGAA GTTTCTAGAT 
TATGGATTTT TTTATTTCGA TGGCAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA 
ACAAACTGCT TGGATTGTCT GGATAGAACA AATAGTGTGC AGGCATTCCT TGGCTTAGAG 
ATGCTCGCTA AGCAGCTGGA AGCTCTGGGC TTGGCTGAGA AGCCTCAGCT GGTGACCCGC 
TTCCAAGAAG TTTTCCGGTC CATGTGGTCT GTGAATGGCG ACTCAATCAG TAAAATATAT 
GCAGGCACCG GGGCCCTGGA AGGGAAGGCC AAGTTAAAAG ATGGTGCGCG ATCTGTCACC 
AGAACCATCC AGAATAACTT CTTCGACAGC TCCAAGCAGG AAGCCATTGA TGTCCTGCTC 
CTGGGAAATA CTCTCAACAG CGATTTAGCT GACAAAGCCC GAGCGCTTCT AACTACTGGA 
AGTTTGCGTG TTTCTGAACA GACATTACAG TCAGCATCTT CCAAAGTCTT AAAGAACATG 
TGTGAGAACT TCTACAAATA CTCAAAGCCC AAGAAGATCC GAGTGTGTGT TGGCACCTGG 
AATGTGAACG GCGGGAAGCA GTTCCGCAGC ATAGCGTTCA AGAACCAGAC GCTCACAGAC 
TGGCTTCTGG ACGCTCCCAA GTTAGCCGGC ATCCAGGAGT TTCAAGATAA GAGAAGTAAG 
CCAACTGATA TATTTGCAAT TGGCTTTGAA GAAATGGTGG AGCTGAATGC TGGGAACATT 
GTGAATGCAA GCACAACCAA CCAGAAGCTG TGGGCAGTGG AGCTGCAGAA GACCATCTCC 
AGGGACAACA AGTATGTCCT GCTGGCCTCG GAGCAGCTGG TGGGTGTCTG CCTGTTTGTC 
TTTATCAGAC CACAGCACGC TCCTTTTATC AGGGATGTTG CAGTTGATAC TGTGAAAACT 
GGCATGGGAG GTGCAACTGG AAACAAGGGC GCCGTTGCAA TTCGGATGCT CTTCCACACC 
ACCAGCCTTT GCTTCGTCTG CAGCCACTTT GCTGCAGGGC AGTCCCAAGT CAAAGAGAGA 
AATGAAGATT TTGTAGAAAT AGCACGAAAA CTGAGTTTCC CCATGGGACG AATGCTCTTC 
TCCCATGACT ATGTATTTTG GTGTGGTGAT TTCAACTATC GAATTGACCT TCCGAATGAA 
GAGGTTAAAG AGCTCATAAG ACAACAAAAC TGGGATTCTC TGATCGCTGG GGATCAGCTC 
ATCAACCAGA AAAATGCAGG ACAGATCTTT AGAGGATTCT TAGAGGGAAA AGTAACGTTT 
GCTCCAACCT ATAAATATGA CTTGTTTTCT GAAGACTATG ACACCAGTGA GAAGTGTCGC 
ACCCCTGCTT GGACAGACCG TGTCCTCTGG AGAAGGAGGA AGTGGCCTTT TGACAGATCA 
GCTGAAGATT TAGATCTCCT GAATGCTAGT TTCCAAGATG AAAGTAAAAT TCTTTATACA 
TGGACCCCTG GCACCTTGCT GCACTATGGA AGAGCCGAGC TGAAGACTTC TGACCATAGG 
CCCGTCGTTG CCTTGATTGA CATAGATATA TTTGAAGTTG AAGCTGAAGA GAGACAAAAA 
ATTTATAAAG AAGTTATTGC CGTCCAGGGC CCACCAGATG GCACAGTGCT GGTCTCAATC 
AAAAGTTCTG CACAAGAAAG TACTTTTTTT GATGATGCTT TGATTGATGA GCTTCTGCGG 
CAGTTTGCAC ACTTTGGTGA AGTTATACTC ATAAGATTTG TAGAAGATAA AATGTGGGTT 
ACATTTTTGG AGGGAAGCTC TGCCTTGAAT GCTCTGAGCC TAAATGGGAA AGAGCTATTA 
AATCGGACTA TAACAATTAC TTTAAAAAGT CCAGACTGGA TCAAACATTT GGAAGAAGAG 
ATGAGTTTAG AGAAAATCAG TGTCACGTTG CCCTCATCAG CAAGCTCCAC CCTGCTTGGT 
GAAGACGCAG AGGTCGCAGC AGACTTTGAC ATGGAAGGTG ACGTCGATGA CTACAGCGCT 
GAAGTAGAGG AGCTTCTTCC TCAGCATCTG CAGCCGTCCT CAAGTTCTGG CCTGGGCACT 
TCTCCAAGTT CTTCACCCCG GACCAGTCCC TGCCAGTCCC CCACAGTACC AGAGTACTCC 
GCCCCTTCTC TTCCCATCAG ACCTAGCCGA GCACCGTCAA GAACTCCAGG ACCTCCAAGT 
TCGCAGGGCT CTCCAGTTGA CACTCAGCCA GCGGCCCAGA AGGATTCTTC CCAGACTCTA 
GAGCCCAAGA GACCACCCCC TCCCCGCCCA GTCGCTCCTC CGGCACGTCC TGCCCCACCA 
CAGAGACCAC CTCCACCTTC AGGGGCTAGG AGTCCTGCGC CTGCTAGAAA AGAATTTGGA 
GGTGTTGGAG CCCCTCCCAG TCCCGGGGTT GCTAGGAGAG AGATAGAAGC ACCCAAAAGC 
CCTGGAACAG CACGGAAAGA TAATATAGGG CGTAATCAGC CTTCCCCTCA AGCCGGACTT 
GCAGGCCCAG GACCCGCTGG ATACGGTGCG GCTAGACCGA CAATTCCAGC TCGTGCTGGA 
GTGATCAGCG CCCCTCAGAG CCAGGCTCGG GTATGTGCTG GAAGGCCGAC TCCTGACAGC 
CAAAGCAAGC CCTCGGAGAC ATTGAAAGGT CCTGCTGTCC TTCCAGAACC ACTGAAGCCT 
CAGGCTGCAT TTCCTCAGCA GCCTTCTCTG CCCACACCTG CTCAAAAGTT ACAGGACCCG 
CTTGTCCCCA TAGCAGCACC TACTATGCCT CCCTCTGGCC CCCAACCAAA TTTGGAAACC 
CCCCCACAGC CCCCACCTCG GAGCAGGTCA TCTCAAAGCT TGCCTTCAGA CTCCTCACCA 
CAGCTGCAGG TAAAAATAAA TGGCATTTCT GGTGTCAAAC AAGAGCCAAC ATTGAAGAGT 
GACCCATTTG AAGATCTCTC ACTTAGTGTG CTTGCTGTAT CAAAGGCTCA GCCTTCTGTT 
CAGATTTCAC CTGTTCTAAC CCCAGACCCA AAGATGTTGA TTCAGTTGCC TTCTGCATCG 
CAAAGTCAAG TTAACCCTTT GAGTTCTGTA AGTTGCATGC CAACCAGGCC TCCAGGTCCA 
GAGGAAAGCA AGTCACAGGA GAGTATGGGC AGTTCTGCAA ACCCGTTTCC CAGCCTGCCC 
TGCCGGAACC CTTTTACAGA CAGGACTGCT GCCCCTGGAA ACCCATTTAG AGTTCAGTCC 
CAAGAATCAG AGGCAACTTC TTGGCTCTCC AAAGAAGAGC CTGTTCCCAA CAGTCCCTTC 
CCTCCTCTCA TGCCTCTCAG TCATGACACG AGCAAGGCTT CAAGTTCGCT TGGTGGCTTT 
GAGGACAATT TTGATCTGCA GAGCCAGTCT ACAGTAAAAA CAAGCAACCC CAAAGGATGG 
GTAACCTTTG ACGAAGACGA CAACTTTCCT ACAACAGGAA AGTCAAAGTC AGTTTGTCCA 
GACTTAGTGG GTAACGCGCC AGCTTCATTT GATGATGACT GGAGTAAAGG TGCGAGCGTT 
TCTTTCTGTG TGTTGCCAGC CAGAAGGCCA CCACCACCAC CACCTCCTGT CCCTCTGCTA 
CCACCTGGCA CAACCTCATC TGCAGGACCT AGCACAACCC TGCCATCTAA GGCACCTTCC 
ACATTGGACT TCACGGAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104765.1
CDS	146..4849
Translation	MAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_030248905.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248905.1

ORF Insert Sequence:

ATGGCGTTCA GCAAAGGATT TCGAATCTAC CACAAATTGG ATCCCCCACC GTTCAGCCTC 
ATAGTGGAAA CTAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTGGCTGTG 
CTCTCATCTG CAGAAAAGGA GGCTATTAAA GGCACATATG CCAAAGTACT GGATGCATAC 
GGACTTTTGG GAGTTTTACG ATTAAATCTT GGTGATACCA TGCTGCACTA TCTGGTCCTA 
GTCACTGGAT GCATGTCTGT GGGGAAAATC CAAGAATCTG AAGTTTTCCG AGTTACTTCC 
ACTGAGTTTA TATCATTGCG AGTTGATGCT TCAGATGAGG ACCGCATTTC GGAAGTACGA 
AAAGTTTTGA ACTCGGGAAA CTTTTATTTT GCATGGTCTG CATCTGGAGT CAGCTTAGAT 
CTGAGTCTGA ATGCACATCG AAGCATGCAA GAGCACACAA CCGACAATAG GTTTTTCTGG 
AATCAGTCTC TGCATTTGCA TCTCAAGCAC TATGGTGTGA ACTGCGATGA CTGGTTATTA 
CGGCTCATGT GTGGGGGAGT AGAGATTAGA ACCATTTATG CTGCCCATAA ACAGGCAAAA 
GCTTGCCTCA TCTCAAGACT AAGCTGTGAA CGAGCTGGGA CAAGGTTCAA TGTCCGGGGA 
ACCAACGATG ATGGTCATGT TGCCAACTTT GTAGAGACAG AGCAGGTTAT ATACTTAGAT 
GACTGTGTCT CTTCCTTCAT ACAAATCCGA GGATCTGTTC CATTGTTCTG GGAGCAACCA 
GGGTTGCAAG TGGGATCCCA TCGTGTTCGT ATGTCAAGGG GATTTGAAGC CAATGCGCCT 
GCCTTTGACA GGCATTTCCG AACACTTAAG GACTTATATG GTAAACAGAT AGTAGTGAAT 
CTGCTTGGGT CTAAGGAAGG CGAGCATATG CTGAGCAAGG CTTTCCAGAG TCATTTAAAA 
GCCTCTGAAC ACGCTTCTGA TATCCACATG GTGTCTTTTG ACTATCATCA AATGGTTAAA 
GGAGGAAAGG CAGAAAAACT ACACAGTATT CTCAAACCAC AAGTCCAGAA GTTTCTAGAT 
TATGGATTTT TTTATTTCGA TGGCAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA 
ACAAACTGCT TGGATTGTCT GGATAGAACA AATAGTGTGC AGGCATTCCT TGGCTTAGAG 
ATGCTCGCTA AGCAGCTGGA AGCTCTGGGC TTGGCTGAGA AGCCTCAGCT GGTGACCCGC 
TTCCAAGAAG TTTTCCGGTC CATGTGGTCT GTGAATGGCG ACTCAATCAG TAAAATATAT 
GCAGGCACCG GGGCCCTGGA AGGGAAGGCC AAGTTAAAAG ATGGTGCGCG ATCTGTCACC 
AGAACCATCC AGAATAACTT CTTCGACAGC TCCAAGCAGG AAGCCATTGA TGTCCTGCTC 
CTGGGAAATA CTCTCAACAG CGATTTAGCT GACAAAGCCC GAGCGCTTCT AACTACTGGA 
AGTTTGCGTG TTTCTGAACA GACATTACAG TCAGCATCTT CCAAAGTCTT AAAGAACATG 
TGTGAGAACT TCTACAAATA CTCAAAGCCC AAGAAGATCC GAGTGTGTGT TGGCACCTGG 
AATGTGAACG GCGGGAAGCA GTTCCGCAGC ATAGCGTTCA AGAACCAGAC GCTCACAGAC 
TGGCTTCTGG ACGCTCCCAA GTTAGCCGGC ATCCAGGAGT TTCAAGATAA GAGAAGTAAG 
CCAACTGATA TATTTGCAAT TGGCTTTGAA GAAATGGTGG AGCTGAATGC TGGGAACATT 
GTGAATGCAA GCACAACCAA CCAGAAGCTG TGGGCAGTGG AGCTGCAGAA GACCATCTCC 
AGGGACAACA AGTATGTCCT GCTGGCCTCG GAGCAGCTGG TGGGTGTCTG CCTGTTTGTC 
TTTATCAGAC CACAGCACGC TCCTTTTATC AGGGATGTTG CAGTTGATAC TGTGAAAACT 
GGCATGGGAG GTGCAACTGG AAACAAGGGC GCCGTTGCAA TTCGGATGCT CTTCCACACC 
ACCAGCCTTT GCTTCGTCTG CAGCCACTTT GCTGCAGGGC AGTCCCAAGT CAAAGAGAGA 
AATGAAGATT TTGTAGAAAT AGCACGAAAA CTGAGTTTCC CCATGGGACG AATGCTCTTC 
TCCCATGACT ATGTATTTTG GTGTGGTGAT TTCAACTATC GAATTGACCT TCCGAATGAA 
GAGGTTAAAG AGCTCATAAG ACAACAAAAC TGGGATTCTC TGATCGCTGG GGATCAGCTC 
ATCAACCAGA AAAATGCAGG ACAGATCTTT AGAGGATTCT TAGAGGGAAA AGTAACGTTT 
GCTCCAACCT ATAAATATGA CTTGTTTTCT GAAGACTATG ACACCAGTGA GAAGTGTCGC 
ACCCCTGCTT GGACAGACCG TGTCCTCTGG AGAAGGAGGA AGTGGCCTTT TGACAGATCA 
GCTGAAGATT TAGATCTCCT GAATGCTAGT TTCCAAGATG AAAGTAAAAT TCTTTATACA 
TGGACCCCTG GCACCTTGCT GCACTATGGA AGAGCCGAGC TGAAGACTTC TGACCATAGG 
CCCGTCGTTG CCTTGATTGA CATAGATATA TTTGAAGTTG AAGCTGAAGA GAGACAAAAA 
ATTTATAAAG AAGTTATTGC CGTCCAGGGC CCACCAGATG GCACAGTGCT GGTCTCAATC 
AAAAGTTCTG CACAAGAAAG TACTTTTTTT GATGATGCTT TGATTGATGA GCTTCTGCGG 
CAGTTTGCAC ACTTTGGTGA AGTTATACTC ATAAGATTTG TAGAAGATAA AATGTGGGTT 
ACATTTTTGG AGGGAAGCTC TGCCTTGAAT GCTCTGAGCC TAAATGGGAA AGAGCTATTA 
AATCGGACTA TAACAATTAC TTTAAAAAGT CCAGACTGGA TCAAACATTT GGAAGAAGAG 
ATGAGTTTAG AGAAAATCAG TGTCACGTTG CCCTCATCAG CAAGCTCCAC CCTGCTTGGT 
GAAGACGCAG AGGTCGCAGC AGACTTTGAC ATGGAAGGTG ACGTCGATGA CTACAGCGCT 
GAAGTAGAGG AGCTTCTTCC TCAGCATCTG CAGCCGTCCT CAAGTTCTGG CCTGGGCACT 
TCTCCAAGTT CTTCACCCCG GACCAGTCCC TGCCAGTCCC CCACAGTACC AGAGTACTCC 
GCCCCTTCTC TTCCCATCAG ACCTAGCCGA GCACCGTCAA GAACTCCAGG ACCTCCAAGT 
TCGCAGGGCT CTCCAGTTGA CACTCAGCCA GCGGCCCAGA AGGATTCTTC CCAGACTCTA 
GAGCCCAAGA GACCACCCCC TCCCCGCCCA GTCGCTCCTC CGGCACGTCC TGCCCCACCA 
CAGAGACCAC CTCCACCTTC AGGGGCTAGG AGTCCTGCGC CTGCTAGAAA AGAATTTGGA 
GGTGTTGGAG CCCCTCCCAG TCCCGGGGTT GCTAGGAGAG AGATAGAAGC ACCCAAAAGC 
CCTGGAACAG CACGGAAAGA TAATATAGGG CGTAATCAGC CTTCCCCTCA AGCCGGACTT 
GCAGGCCCAG GACCCGCTGG ATACGGTGCG GCTAGACCGA CAATTCCAGC TCGTGCTGGA 
GTGATCAGCG CCCCTCAGAG CCAGGCTCGG GTATGTGCTG GAAGGCCGAC TCCTGACAGC 
CAAAGCAAGC CCTCGGAGAC ATTGAAAGGT CCTGCTGTCC TTCCAGAACC ACTGAAGCCT 
CAGGCTGCAT TTCCTCAGCA GCCTTCTCTG CCCACACCTG CTCAAAAGTT ACAGGACCCG 
CTTGTCCCCA TAGCAGCACC TACTATGCCT CCCTCTGGCC CCCAACCAAA TTTGGAAACC 
CCCCCACAGC CCCCACCTCG GAGCAGGTCA TCTCAAAGCT TGCCTTCAGA CTCCTCACCA 
CAGCTGCAGG TAAAAATAAA TGGCATTTCT GGTGTCAAAC AAGAGCCAAC ATTGAAGAGT 
GACCCATTTG AAGATCTCTC ACTTAGTGTG CTTGCTGTAT CAAAGGCTCA GCCTTCTGTT 
CAGATTTCAC CTGTTCTAAC CCCAGACCCA AAGATGTTGA TTCAGTTGCC TTCTGCATCG 
CAAAGTCAAG TTAACCCTTT GAGTTCTGTA AGTTGCATGC CAACCAGGCC TCCAGGTCCA 
GAGGAAAGCA AGTCACAGGA GAGTATGGGC AGTTCTGCAA ACCCGTTTCC CAGCCTGCCC 
TGCCGGAACC CTTTTACAGA CAGGACTGCT GCCCCTGGAA ACCCATTTAG AGTTCAGTCC 
CAAGAATCAG AGGCAACTTC TTGGCTCTCC AAAGAAGAGC CTGTTCCCAA CAGTCCCTTC 
CCTCCTCTCA TGCCTCTCAG TCATGACACG AGCAAGGCTT CAAGTTCGCT TGGTGGCTTT 
GAGGACAATT TTGATCTGCA GAGCCAGTCT ACAGTAAAAA CAAGCAACCC CAAAGGATGG 
GTAACCTTTG ACGAAGACGA CAACTTTCCT ACAACAGGAA AGTCAAAGTC AGTTTGTCCA 
GACTTAGTGG GTAACGCGCC AGCTTCATTT GATGATGACT GGAGTAAAGG TGCGAGCGTT 
TCTTTCTGTG TGTTGCCAGC CAGAAGGCCA CCACCACCAC CACCTCCTGT CCCTCTGCTA 
CCACCTGGCA CAACCTCATC TGCAGGACCT AGCACAACCC TGCCATCTAA GGCACCTTCC 
ACATTGGACT TCACGGAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_030248906.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104766.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OMu86849
Clone ID Related Accession (Same CDS sequence)	XM_030248906.1
Accession Version	XM_030248906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTCA GCAAAGGATT TCGAATCTAC CACAAATTGG ATCCCCCACC GTTCAGCCTC 
ATAGTGGAAA CTAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTGGCTGTG 
CTCTCATCTG CAGAAAAGGA GGCTATTAAA GGCACATATG CCAAAGTACT GGATGCATAC 
GGACTTTTGG GAGTTTTACG ATTAAATCTT GGTGATACCA TGCTGCACTA TCTGGTCCTA 
GTCACTGGAT GCATGTCTGT GGGGAAAATC CAAGAATCTG AAGTTTTCCG AGTTACTTCC 
ACTGAGTTTA TATCATTGCG AGTTGATGCT TCAGATGAGG ACCGCATTTC GGAAGTACGA 
AAAGTTTTGA ACTCGGGAAA CTTTTATTTT GCATGGTCTG CATCTGGAGT CAGCTTAGAT 
CTGAGTCTGA ATGCACATCG AAGCATGCAA GAGCACACAA CCGACAATAG GTTTTTCTGG 
AATCAGTCTC TGCATTTGCA TCTCAAGCAC TATGGTGTGA ACTGCGATGA CTGGTTATTA 
CGGCTCATGT GTGGGGGAGT AGAGATTAGA ACCATTTATG CTGCCCATAA ACAGGCAAAA 
GCTTGCCTCA TCTCAAGACT AAGCTGTGAA CGAGCTGGGA CAAGGTTCAA TGTCCGGGGA 
ACCAACGATG ATGGTCATGT TGCCAACTTT GTAGAGACAG AGCAGGTTAT ATACTTAGAT 
GACTGTGTCT CTTCCTTCAT ACAAATCCGA GGATCTGTTC CATTGTTCTG GGAGCAACCA 
GGGTTGCAAG TGGGATCCCA TCGTGTTCGT ATGTCAAGGG GATTTGAAGC CAATGCGCCT 
GCCTTTGACA GGCATTTCCG AACACTTAAG GACTTATATG GTAAACAGAT AGTAGTGAAT 
CTGCTTGGGT CTAAGGAAGG CGAGCATATG CTGAGCAAGG CTTTCCAGAG TCATTTAAAA 
GCCTCTGAAC ACGCTTCTGA TATCCACATG GTGTCTTTTG ACTATCATCA AATGGTTAAA 
GGAGGAAAGG CAGAAAAACT ACACAGTATT CTCAAACCAC AAGTCCAGAA GTTTCTAGAT 
TATGGATTTT TTTATTTCGA TGGCAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA 
ACAAACTGCT TGGATTGTCT GGATAGAACA AATAGTGTGC AGGCATTCCT TGGCTTAGAG 
ATGCTCGCTA AGCAGCTGGA AGCTCTGGGC TTGGCTGAGA AGCCTCAGCT GGTGACCCGC 
TTCCAAGAAG TTTTCCGGTC CATGTGGTCT GTGAATGGCG ACTCAATCAG TAAAATATAT 
GCAGGCACCG GGGCCCTGGA AGGGAAGGCC AAGTTAAAAG ATGGTGCGCG ATCTGTCACC 
AGAACCATCC AGAATAACTT CTTCGACAGC TCCAAGCAGG AAGCCATTGA TGTCCTGCTC 
CTGGGAAATA CTCTCAACAG CGATTTAGCT GACAAAGCCC GAGCGCTTCT AACTACTGGA 
AGTTTGCGTG CATCTTCCAA AGTCTTAAAG AACATGTGTG AGAACTTCTA CAAATACTCA 
AAGCCCAAGA AGATCCGAGT GTGTGTTGGC ACCTGGAATG TGAACGGCGG GAAGCAGTTC 
CGCAGCATAG CGTTCAAGAA CCAGACGCTC ACAGACTGGC TTCTGGACGC TCCCAAGTTA 
GCCGGCATCC AGGAGTTTCA AGATAAGAGA AGTAAGCCAA CTGATATATT TGCAATTGGC 
TTTGAAGAAA TGGTGGAGCT GAATGCTGGG AACATTGTGA ATGCAAGCAC AACCAACCAG 
AAGCTGTGGG CAGTGGAGCT GCAGAAGACC ATCTCCAGGG ACAACAAGTA TGTCCTGCTG 
GCCTCGGAGC AGCTGGTGGG TGTCTGCCTG TTTGTCTTTA TCAGACCACA GCACGCTCCT 
TTTATCAGGG ATGTTGCAGT TGATACTGTG AAAACTGGCA TGGGAGGTGC AACTGGAAAC 
AAGGGCGCCG TTGCAATTCG GATGCTCTTC CACACCACCA GCCTTTGCTT CGTCTGCAGC 
CACTTTGCTG CAGGGCAGTC CCAAGTCAAA GAGAGAAATG AAGATTTTGT AGAAATAGCA 
CGAAAACTGA GTTTCCCCAT GGGACGAATG CTCTTCTCCC ATGACTATGT ATTTTGGTGT 
GGTGATTTCA ACTATCGAAT TGACCTTCCG AATGAAGAGG TTAAAGAGCT CATAAGACAA 
CAAAACTGGG ATTCTCTGAT CGCTGGGGAT CAGCTCATCA ACCAGAAAAA TGCAGGACAG 
ATCTTTAGAG GATTCTTAGA GGGAAAAGTA ACGTTTGCTC CAACCTATAA ATATGACTTG 
TTTTCTGAAG ACTATGACAC CAGTGAGAAG TGTCGCACCC CTGCTTGGAC AGACCGTGTC 
CTCTGGAGAA GGAGGAAGTG GCCTTTTGAC AGATCAGCTG AAGATTTAGA TCTCCTGAAT 
GCTAGTTTCC AAGATGAAAG TAAAATTCTT TATACATGGA CCCCTGGCAC CTTGCTGCAC 
TATGGAAGAG CCGAGCTGAA GACTTCTGAC CATAGGCCCG TCGTTGCCTT GATTGACATA 
GATATATTTG AAGTTGAAGC TGAAGAGAGA CAAAAAATTT ATAAAGAAGT TATTGCCGTC 
CAGGGCCCAC CAGATGGCAC AGTGCTGGTC TCAATCAAAA GTTCTGCACA AGAAAGTACT 
TTTTTTGATG ATGCTTTGAT TGATGAGCTT CTGCGGCAGT TTGCACACTT TGGTGAAGTT 
ATACTCATAA GATTTGTAGA AGATAAAATG TGGGTTACAT TTTTGGAGGG AAGCTCTGCC 
TTGAATGCTC TGAGCCTAAA TGGGAAAGAG CTATTAAATC GGACTATAAC AATTACTTTA 
AAAAGTCCAG ACTGGATCAA ACATTTGGAA GAAGAGATGA GTTTAGAGAA AATCAGTGTC 
ACGTTGCCCT CATCAGCAAG CTCCACCCTG CTTGGTGAAG ACGCAGAGGT CGCAGCAGAC 
TTTGACATGG AAGGTGACGT CGATGACTAC AGCGCTGAAG TAGAGGAGCT TCTTCCTCAG 
CATCTGCAGC CGTCCTCAAG TTCTGGCCTG GGCACTTCTC CAAGTTCTTC ACCCCGGACC 
AGTCCCTGCC AGTCCCCCAC AGTACCAGAG TACTCCGCCC CTTCTCTTCC CATCAGACCT 
AGCCGAGCAC CGTCAAGAAC TCCAGGACCT CCAAGTTCGC AGGGCTCTCC AGTTGACACT 
CAGCCAGCGG CCCAGAAGGA TTCTTCCCAG ACTCTAGAGC CCAAGAGACC ACCCCCTCCC 
CGCCCAGTCG CTCCTCCGGC ACGTCCTGCC CCACCACAGA GACCACCTCC ACCTTCAGGG 
GCTAGGAGTC CTGCGCCTGC TAGAAAAGAA TTTGGAGGTG TTGGAGCCCC TCCCAGTCCC 
GGGGTTGCTA GGAGAGAGAT AGAAGCACCC AAAAGCCCTG GAACAGCACG GAAAGATAAT 
ATAGGGCGTA ATCAGCCTTC CCCTCAAGCC GGACTTGCAG GCCCAGGACC CGCTGGATAC 
GGTGCGGCTA GACCGACAAT TCCAGCTCGT GCTGGAGTGA TCAGCGCCCC TCAGAGCCAG 
GCTCGGGTAT GTGCTGGAAG GCCGACTCCT GACAGCCAAA GCAAGCCCTC GGAGACATTG 
AAAGGTCCTG CTGTCCTTCC AGAACCACTG AAGCCTCAGG CTGCATTTCC TCAGCAGCCT 
TCTCTGCCCA CACCTGCTCA AAAGTTACAG GACCCGCTTG TCCCCATAGC AGCACCTACT 
ATGCCTCCCT CTGGCCCCCA ACCAAATTTG GAAACCCCCC CACAGCCCCC ACCTCGGAGC 
AGGTCATCTC AAAGCTTGCC TTCAGACTCC TCACCACAGC TGCAGGTAAA AATAAATGGC 
ATTTCTGGTG TCAAACAAGA GCCAACATTG AAGAGTGACC CATTTGAAGA TCTCTCACTT 
AGTGTGCTTG CTGTATCAAA GGCTCAGCCT TCTGTTCAGA TTTCACCTGT TCTAACCCCA 
GACCCAAAGA TGTTGATTCA GTTGCCTTCT GCATCGCAAA GTCAAGTTAA CCCTTTGAGT 
TCTGTAAGTT GCATGCCAAC CAGGCCTCCA GGTCCAGAGG AAAGCAAGTC ACAGGAGAGT 
ATGGGCAGTT CTGCAAACCC GTTTCCCAGC CTGCCCTGCC GGAACCCTTT TACAGACAGG 
ACTGCTGCCC CTGGAAACCC ATTTAGAGTT CAGTCCCAAG AATCAGAGGC AACTTCTTGG 
CTCTCCAAAG AAGAGCCTGT TCCCAACAGT CCCTTCCCTC CTCTCATGCC TCTCAGTCAT 
GACACGAGCA AGGCTTCAAG TTCGCTTGGT GGCTTTGAGG ACAATTTTGA TCTGCAGAGC 
CAGTCTACAG TAAAAACAAG CAACCCCAAA GGATGGGTAA CCTTTGACGA AGACGACAAC 
TTTCCTACAA CAGGAAAGTC AAAGTCAGTT TGTCCAGACT TAGTGGGTAA CGCGCCAGCT 
TCATTTGATG ATGACTGGAG TAAAGGTGCG AGCGTTTCTT TCTGTGTGTT GCCAGCCAGA 
AGGCCACCAC CACCACCACC TCCTGTCCCT CTGCTACCAC CTGGCACAAC CTCATCTGCA 
GGACCTAGCA CAACCCTGCC ATCTAAGGCA CCTTCCACAT TGGACTTCAC GGAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104766.1
CDS	146..4825
Translation	MAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_030248906.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248906.1

ORF Insert Sequence:

ATGGCGTTCA GCAAAGGATT TCGAATCTAC CACAAATTGG ATCCCCCACC GTTCAGCCTC 
ATAGTGGAAA CTAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTGGCTGTG 
CTCTCATCTG CAGAAAAGGA GGCTATTAAA GGCACATATG CCAAAGTACT GGATGCATAC 
GGACTTTTGG GAGTTTTACG ATTAAATCTT GGTGATACCA TGCTGCACTA TCTGGTCCTA 
GTCACTGGAT GCATGTCTGT GGGGAAAATC CAAGAATCTG AAGTTTTCCG AGTTACTTCC 
ACTGAGTTTA TATCATTGCG AGTTGATGCT TCAGATGAGG ACCGCATTTC GGAAGTACGA 
AAAGTTTTGA ACTCGGGAAA CTTTTATTTT GCATGGTCTG CATCTGGAGT CAGCTTAGAT 
CTGAGTCTGA ATGCACATCG AAGCATGCAA GAGCACACAA CCGACAATAG GTTTTTCTGG 
AATCAGTCTC TGCATTTGCA TCTCAAGCAC TATGGTGTGA ACTGCGATGA CTGGTTATTA 
CGGCTCATGT GTGGGGGAGT AGAGATTAGA ACCATTTATG CTGCCCATAA ACAGGCAAAA 
GCTTGCCTCA TCTCAAGACT AAGCTGTGAA CGAGCTGGGA CAAGGTTCAA TGTCCGGGGA 
ACCAACGATG ATGGTCATGT TGCCAACTTT GTAGAGACAG AGCAGGTTAT ATACTTAGAT 
GACTGTGTCT CTTCCTTCAT ACAAATCCGA GGATCTGTTC CATTGTTCTG GGAGCAACCA 
GGGTTGCAAG TGGGATCCCA TCGTGTTCGT ATGTCAAGGG GATTTGAAGC CAATGCGCCT 
GCCTTTGACA GGCATTTCCG AACACTTAAG GACTTATATG GTAAACAGAT AGTAGTGAAT 
CTGCTTGGGT CTAAGGAAGG CGAGCATATG CTGAGCAAGG CTTTCCAGAG TCATTTAAAA 
GCCTCTGAAC ACGCTTCTGA TATCCACATG GTGTCTTTTG ACTATCATCA AATGGTTAAA 
GGAGGAAAGG CAGAAAAACT ACACAGTATT CTCAAACCAC AAGTCCAGAA GTTTCTAGAT 
TATGGATTTT TTTATTTCGA TGGCAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA 
ACAAACTGCT TGGATTGTCT GGATAGAACA AATAGTGTGC AGGCATTCCT TGGCTTAGAG 
ATGCTCGCTA AGCAGCTGGA AGCTCTGGGC TTGGCTGAGA AGCCTCAGCT GGTGACCCGC 
TTCCAAGAAG TTTTCCGGTC CATGTGGTCT GTGAATGGCG ACTCAATCAG TAAAATATAT 
GCAGGCACCG GGGCCCTGGA AGGGAAGGCC AAGTTAAAAG ATGGTGCGCG ATCTGTCACC 
AGAACCATCC AGAATAACTT CTTCGACAGC TCCAAGCAGG AAGCCATTGA TGTCCTGCTC 
CTGGGAAATA CTCTCAACAG CGATTTAGCT GACAAAGCCC GAGCGCTTCT AACTACTGGA 
AGTTTGCGTG CATCTTCCAA AGTCTTAAAG AACATGTGTG AGAACTTCTA CAAATACTCA 
AAGCCCAAGA AGATCCGAGT GTGTGTTGGC ACCTGGAATG TGAACGGCGG GAAGCAGTTC 
CGCAGCATAG CGTTCAAGAA CCAGACGCTC ACAGACTGGC TTCTGGACGC TCCCAAGTTA 
GCCGGCATCC AGGAGTTTCA AGATAAGAGA AGTAAGCCAA CTGATATATT TGCAATTGGC 
TTTGAAGAAA TGGTGGAGCT GAATGCTGGG AACATTGTGA ATGCAAGCAC AACCAACCAG 
AAGCTGTGGG CAGTGGAGCT GCAGAAGACC ATCTCCAGGG ACAACAAGTA TGTCCTGCTG 
GCCTCGGAGC AGCTGGTGGG TGTCTGCCTG TTTGTCTTTA TCAGACCACA GCACGCTCCT 
TTTATCAGGG ATGTTGCAGT TGATACTGTG AAAACTGGCA TGGGAGGTGC AACTGGAAAC 
AAGGGCGCCG TTGCAATTCG GATGCTCTTC CACACCACCA GCCTTTGCTT CGTCTGCAGC 
CACTTTGCTG CAGGGCAGTC CCAAGTCAAA GAGAGAAATG AAGATTTTGT AGAAATAGCA 
CGAAAACTGA GTTTCCCCAT GGGACGAATG CTCTTCTCCC ATGACTATGT ATTTTGGTGT 
GGTGATTTCA ACTATCGAAT TGACCTTCCG AATGAAGAGG TTAAAGAGCT CATAAGACAA 
CAAAACTGGG ATTCTCTGAT CGCTGGGGAT CAGCTCATCA ACCAGAAAAA TGCAGGACAG 
ATCTTTAGAG GATTCTTAGA GGGAAAAGTA ACGTTTGCTC CAACCTATAA ATATGACTTG 
TTTTCTGAAG ACTATGACAC CAGTGAGAAG TGTCGCACCC CTGCTTGGAC AGACCGTGTC 
CTCTGGAGAA GGAGGAAGTG GCCTTTTGAC AGATCAGCTG AAGATTTAGA TCTCCTGAAT 
GCTAGTTTCC AAGATGAAAG TAAAATTCTT TATACATGGA CCCCTGGCAC CTTGCTGCAC 
TATGGAAGAG CCGAGCTGAA GACTTCTGAC CATAGGCCCG TCGTTGCCTT GATTGACATA 
GATATATTTG AAGTTGAAGC TGAAGAGAGA CAAAAAATTT ATAAAGAAGT TATTGCCGTC 
CAGGGCCCAC CAGATGGCAC AGTGCTGGTC TCAATCAAAA GTTCTGCACA AGAAAGTACT 
TTTTTTGATG ATGCTTTGAT TGATGAGCTT CTGCGGCAGT TTGCACACTT TGGTGAAGTT 
ATACTCATAA GATTTGTAGA AGATAAAATG TGGGTTACAT TTTTGGAGGG AAGCTCTGCC 
TTGAATGCTC TGAGCCTAAA TGGGAAAGAG CTATTAAATC GGACTATAAC AATTACTTTA 
AAAAGTCCAG ACTGGATCAA ACATTTGGAA GAAGAGATGA GTTTAGAGAA AATCAGTGTC 
ACGTTGCCCT CATCAGCAAG CTCCACCCTG CTTGGTGAAG ACGCAGAGGT CGCAGCAGAC 
TTTGACATGG AAGGTGACGT CGATGACTAC AGCGCTGAAG TAGAGGAGCT TCTTCCTCAG 
CATCTGCAGC CGTCCTCAAG TTCTGGCCTG GGCACTTCTC CAAGTTCTTC ACCCCGGACC 
AGTCCCTGCC AGTCCCCCAC AGTACCAGAG TACTCCGCCC CTTCTCTTCC CATCAGACCT 
AGCCGAGCAC CGTCAAGAAC TCCAGGACCT CCAAGTTCGC AGGGCTCTCC AGTTGACACT 
CAGCCAGCGG CCCAGAAGGA TTCTTCCCAG ACTCTAGAGC CCAAGAGACC ACCCCCTCCC 
CGCCCAGTCG CTCCTCCGGC ACGTCCTGCC CCACCACAGA GACCACCTCC ACCTTCAGGG 
GCTAGGAGTC CTGCGCCTGC TAGAAAAGAA TTTGGAGGTG TTGGAGCCCC TCCCAGTCCC 
GGGGTTGCTA GGAGAGAGAT AGAAGCACCC AAAAGCCCTG GAACAGCACG GAAAGATAAT 
ATAGGGCGTA ATCAGCCTTC CCCTCAAGCC GGACTTGCAG GCCCAGGACC CGCTGGATAC 
GGTGCGGCTA GACCGACAAT TCCAGCTCGT GCTGGAGTGA TCAGCGCCCC TCAGAGCCAG 
GCTCGGGTAT GTGCTGGAAG GCCGACTCCT GACAGCCAAA GCAAGCCCTC GGAGACATTG 
AAAGGTCCTG CTGTCCTTCC AGAACCACTG AAGCCTCAGG CTGCATTTCC TCAGCAGCCT 
TCTCTGCCCA CACCTGCTCA AAAGTTACAG GACCCGCTTG TCCCCATAGC AGCACCTACT 
ATGCCTCCCT CTGGCCCCCA ACCAAATTTG GAAACCCCCC CACAGCCCCC ACCTCGGAGC 
AGGTCATCTC AAAGCTTGCC TTCAGACTCC TCACCACAGC TGCAGGTAAA AATAAATGGC 
ATTTCTGGTG TCAAACAAGA GCCAACATTG AAGAGTGACC CATTTGAAGA TCTCTCACTT 
AGTGTGCTTG CTGTATCAAA GGCTCAGCCT TCTGTTCAGA TTTCACCTGT TCTAACCCCA 
GACCCAAAGA TGTTGATTCA GTTGCCTTCT GCATCGCAAA GTCAAGTTAA CCCTTTGAGT 
TCTGTAAGTT GCATGCCAAC CAGGCCTCCA GGTCCAGAGG AAAGCAAGTC ACAGGAGAGT 
ATGGGCAGTT CTGCAAACCC GTTTCCCAGC CTGCCCTGCC GGAACCCTTT TACAGACAGG 
ACTGCTGCCC CTGGAAACCC ATTTAGAGTT CAGTCCCAAG AATCAGAGGC AACTTCTTGG 
CTCTCCAAAG AAGAGCCTGT TCCCAACAGT CCCTTCCCTC CTCTCATGCC TCTCAGTCAT 
GACACGAGCA AGGCTTCAAG TTCGCTTGGT GGCTTTGAGG ACAATTTTGA TCTGCAGAGC 
CAGTCTACAG TAAAAACAAG CAACCCCAAA GGATGGGTAA CCTTTGACGA AGACGACAAC 
TTTCCTACAA CAGGAAAGTC AAAGTCAGTT TGTCCAGACT TAGTGGGTAA CGCGCCAGCT 
TCATTTGATG ATGACTGGAG TAAAGGTGCG AGCGTTTCTT TCTGTGTGTT GCCAGCCAGA 
AGGCCACCAC CACCACCACC TCCTGTCCCT CTGCTACCAC CTGGCACAAC CTCATCTGCA 
GGACCTAGCA CAACCCTGCC ATCTAAGGCA CCTTCCACAT TGGACTTCAC GGAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( NM_001164483.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157955.1 Mus musculus synaptojanin 1 (Synj1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09829
Clone ID Related Accession (Same CDS sequence)	NM_001164483.1
Accession Version	NM_001164483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW045957.1, AK080946.1, CX239305.1, CF741221.1, BU703932.1, EL606634.1, BY267025.1, CV558169.1 and AC134560.5. On or before Aug 29, 2009 this sequence version replaced XM_973263.2, XM_001481287.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGAAGA GCTGGACCTG CTGGAGCGGA AGTGACGCTT CGGGCGGCTG TAGCGGCGGA 
GGCTGCGGGA GAAGGAGGAG GAGGAGCCGG AGGAAGAGGG CTGCCTCTGA AGAAAGGAGA 
ATGGCGTTCA GCAAAGGATT TCGAATCTAC CACAAATTGG ATCCCCCACC GTTCAGCCTC 
ATAGTGGAAA CTAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTGGCTGTG 
CTCTCATCTG CAGAAAAGGA GGCTATTAAA GGCACATATG CCAAAGTACT GGATGCATAC 
GGACTTTTGG GAGTTTTACG ATTAAATCTT GGTGATACCA TGCTGCACTA TCTGGTCCTA 
GTCACTGGAT GCATGTCTGT GGGGAAAATC CAAGAATCTG AAGTTTTCCG AGTTACTTCC 
ACTGAGTTTA TATCATTGCG AGTTGATGCT TCAGATGAGG ACCGCATTTC GGAAGTACGA 
AAAGTTTTGA ACTCGGGAAA CTTTTATTTT GCATGGTCTG CATCTGGAGT CAGCTTAGAT 
CTGAGTCTGA ATGCACATCG AAGCATGCAA GAGCACACAA CCGACAATAG GTTTTTCTGG 
AATCAGTCTC TGCATTTGCA TCTCAAGCAC TATGGTGTGA ACTGCGATGA CTGGTTATTA 
CGGCTCATGT GTGGGGGAGT AGAGATTAGA ACCATTTATG CTGCCCATAA ACAGGCAAAA 
GCTTGCCTCA TCTCAAGACT AAGCTGTGAA CGAGCTGGGA CAAGGTTCAA TGTCCGGGGA 
ACCAACGATG ATGGTCATGT TGCCAACTTT GTAGAGACAG AGCAGGTTAT ATACTTAGAT 
GACTGTGTCT CTTCCTTCAT ACAAATCCGA GGATCTGTTC CATTGTTCTG GGAGCAACCA 
GGGTTGCAAG TGGGATCCCA TCGTGTTCGT ATGTCAAGGG GATTTGAAGC CAATGCGCCT 
GCCTTTGACA GGCATTTCCG AACACTTAAG GACTTATATG GTAAACAGAT AGTAGTGAAT 
CTGCTTGGGT CTAAGGAAGG CGAGCATATG CTGAGCAAGG CTTTCCAGAG TCATTTAAAA 
GCCTCTGAAC ACGCTTCTGA TATCCACATG GTGTCTTTTG ACTATCATCA AATGGTTAAA 
GGAGGAAAGG CAGAAAAACT ACACAGTATT CTCAAACCAC AAGTCCAGAA GTTTCTAGAT 
TATGGATTTT TTTATTTCGA TGGCAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA 
ACAAACTGCT TGGATTGTCT GGATAGAACA AATAGTGTGC AGGCATTCCT TGGCTTAGAG 
ATGCTCGCTA AGCAGCTGGA AGCTCTGGGC TTGGCTGAGA AGCCTCAGCT GGTGACCCGC 
TTCCAAGAAG TTTTCCGGTC CATGTGGTCT GTGAATGGCG ACTCAATCAG TAAAATATAT 
GCAGGCACCG GGGCCCTGGA AGGGAAGGCC AAGTTAAAAG ATGGTGCGCG ATCTGTCACC 
AGAACCATCC AGAATAACTT CTTCGACAGC TCCAAGCAGG AAGCCATTGA TGTCCTGCTC 
CTGGGAAATA CTCTCAACAG CGATTTAGCT GACAAAGCCC GAGCGCTTCT AACTACTGGA 
AGTTTGCGTG TTTCTGAACA GACATTACAG TCAGCATCTT CCAAAGTCTT AAAGAACATG 
TGTGAGAACT TCTACAAATA CTCAAAGCCC AAGAAGATCC GAGTGTGTGT TGGCACCTGG 
AATGTGAACG GCGGGAAGCA GTTCCGCAGC ATAGCGTTCA AGAACCAGAC GCTCACAGAC 
TGGCTTCTGG ACGCTCCCAA GTTAGCCGGC ATCCAGGAGT TTCAAGATAA GAGAAGTAAG 
CCAACTGATA TATTTGCAAT TGGCTTTGAA GAAATGGTGG AGCTGAATGC TGGGAACATT 
GTGAATGCAA GCACAACCAA CCAGAAGCTG TGGGCAGTGG AGCTGCAGAA GACCATCTCC 
AGGGACAACA AGTATGTCCT GCTGGCCTCG GAGCAGCTGG TGGGTGTCTG CCTGTTTGTC 
TTTATCAGAC CACAGCACGC TCCTTTTATC AGGGATGTTG CAGTTGATAC TGTGAAAACT 
GGCATGGGAG GTGCAACTGG AAACAAGGGC GCCGTTGCAA TTCGGATGCT CTTCCACACC 
ACCAGCCTTT GCTTCGTCTG CAGCCACTTT GCTGCAGGGC AGTCCCAAGT CAAAGAGAGA 
AATGAAGATT TTGTAGAAAT AGCACGAAAA CTGAGTTTCC CCATGGGACG AATGCTCTTC 
TCCCATGACT ATGTATTTTG GTGTGGTGAT TTCAACTATC GAATTGACCT TCCGAATGAA 
GAGGTTAAAG AGCTCATAAG ACAACAAAAC TGGGATTCTC TGATCGCTGG GGATCAGCTC 
ATCAACCAGA AAAATGCAGG ACAGATCTTT AGAGGATTCT TAGAGGGAAA AGTAACGTTT 
GCTCCAACCT ATAAATATGA CTTGTTTTCT GAAGACTATG ACACCAGTGA GAAGTGTCGC 
ACCCCTGCTT GGACAGACCG TGTCCTCTGG AGAAGGAGGA AGTGGCCTTT TGACAGATCA 
GCTGAAGATT TAGATCTCCT GAATGCTAGT TTCCAAGATG AAAGTAAAAT TCTTTATACA 
TGGACCCCTG GCACCTTGCT GCACTATGGA AGAGCCGAGC TGAAGACTTC TGACCATAGG 
CCCGTCGTTG CCTTGATTGA CATAGATATA TTTGAAGTTG AAGCTGAAGA GAGACAAAAA 
ATTTATAAAG AAGTTATTGC CGTCCAGGGC CCACCAGATG GCACAGTGCT GGTCTCAATC 
AAAAGTTCTG CACAAGAAAG TACTTTTTTT GATGATGCTT TGATTGATGA GCTTCTGCGG 
CAGTTTGCAC ACTTTGGTGA AGTTATACTC ATAAGATTTG TAGAAGATAA AATGTGGGTT 
ACATTTTTGG AGGGAAGCTC TGCCTTGAAT GCTCTGAGCC TAAATGGGAA AGAGCTATTA 
AATCGGACTA TAACAATTAC TTTAAAAAGT CCAGACTGGA TCAAACATTT GGAAGAAGAG 
ATGAGTTTAG AGAAAATCAG TGTCACGTTG CCCTCATCAG CAAGCTCCAC CCTGCTTGGT 
GAAGACGCAG AGGTCGCAGC AGACTTTGAC ATGGAAGGTG ACGTCGATGA CTACAGCGCT 
GAAGTAGAGG AGCTTCTTCC TCAGCATCTG CAGCCGTCCT CAAGTTCTGG CCTGGGCACT 
TCTCCAAGTT CTTCACCCCG GACCAGTCCC TGCCAGTCCC CCACAGTACC AGAGTACTCC 
GCCCCTTCTC TTCCCATCAG ACCTAGCCGA GCACCGTCAA GAACTCCAGG ACCTCCAAGT 
TCGCAGGGCT CTCCAGTTGA CACTCAGCCA GCGGCCCAGA AGGATTCTTC CCAGACTCTA 
GAGCCCAAGA GACCACCCCC TCCCCGCCCA GTCGCTCCTC CGGCACGTCC TGCCCCACCA 
CAGAGACCAC CTCCACCTTC AGGGGCTAGG AGTCCTGCGC CTGCTAGAAA AGAATTTGGA 
GGTGTTGGAG CCCCTCCCAG TCCCGGGGTT GCTAGGAGAG AGATAGAAGC ACCCAAAAGC 
CCTGGAACAG CACGGAAAGA TAATATAGGG CGTAATCAGC CTTCCCCTCA AGCCGGACTT 
GCAGGCCCAG GACCCGCTGG ATACGGTGCG GCTAGACCGA CAATTCCAGC TCGTGCTGGA 
GTGATCAGCG CCCCTCAGAG CCAGGCTCGG GTATGTGCTG GAAGGCCGAC TCCTGACAGC 
CAAAGCAAGC CCTCGGAGAC ATTGAAAGGT CCTGCTGTCC TTCCAGAACC ACTGAAGCCT 
CAGGCTGCAT TTCCTCAGCA GCCTTCTCTG CCCACACCTG CTCAAAAGTT ACAGGACCCG 
CTTGTCCCCA TAGCAGCACC TACTATGCCT CCCTCTGGCC CCCAACCAAA TTTGGAAACC 
CCCCCACAGC CCCCACCTCG GAGCAGGTCA TCTCAAAGCT TGCCTTCAGA CTCCTCACCA 
CAGCTGCAGG TAAAAATAAA TGGCATTTCT GGTGTCAAAC AAGAGCCAAC ATTGAAGAGT 
GACCCATTTG AAGATCTCTC ACTTAGTGTG CTTGCTGTAT CAAAGGCTCA GCCTTCTGTT 
CAGATTTCAC CTGTTCTAAC CCCAGACCCA AAGATGTTGA TTCAGTTGCC TTCTGCATCG 
CAAAGTCAAG TTAACCCTTT GAGTTCTGTA AGTTGCATGC CAACCAGGCC TCCAGGTCCA 
GAGGAAAGCA AGTCACAGGA GAGTATGGGC AGTTCTGCAA ACCCGTTTCC CAGCCTGCCC 
TGCCGGAACC CTTTTACAGA CAGGACTGCT GCCCCTGGAA ACCCATTTAG AGTTCAGTCC 
CAAGAATCAG AGGCAACTTC TTGGCTCTCC AAAGAAGAGC CTGTTCCCAA CAGTCCCTTC 
CCTCCTCTCA TGCCTCTCAG TCATGACACG AGCAAGGCTT CAAGTTCGCT TGGTGGCTTT 
GAGGACAATT TTGATCTGCA GAGCCAGTCT ACAGTAAAAA CAAGCAACCC CAAAGGATGG 
GTAACCTTTG ACGAAGACGA CAACTTTCCT ACAACAGGAA AGTCAAAGTC AGTTTGTCCA 
GACTTAGTGG GTAACGCGCC AGCTTCATTT GATGATGACT GGAGTAAAGG TGCGAGCGTT 
TCTTTCTGTG TGTTGCCAGC CAGAAGGCCA CCACCACCAC CACCTCCTGT CCCTCTGCTA 
CCACCTGGCA CAACCTCATC TGCAGGACCT AGCACAACCC TGCCATCTAA GGCACCTTCC 
ACATTGGACT TCACGGAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157955.1
CDS	119..4942
Translation	MRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	NM_001164483.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164483.1

ORF Insert Sequence:

ATGCGGAAGA GCTGGACCTG CTGGAGCGGA AGTGACGCTT CGGGCGGCTG TAGCGGCGGA 
GGCTGCGGGA GAAGGAGGAG GAGGAGCCGG AGGAAGAGGG CTGCCTCTGA AGAAAGGAGA 
ATGGCGTTCA GCAAAGGATT TCGAATCTAC CACAAATTGG ATCCCCCACC GTTCAGCCTC 
ATAGTGGAAA CTAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTGGCTGTG 
CTCTCATCTG CAGAAAAGGA GGCTATTAAA GGCACATATG CCAAAGTACT GGATGCATAC 
GGACTTTTGG GAGTTTTACG ATTAAATCTT GGTGATACCA TGCTGCACTA TCTGGTCCTA 
GTCACTGGAT GCATGTCTGT GGGGAAAATC CAAGAATCTG AAGTTTTCCG AGTTACTTCC 
ACTGAGTTTA TATCATTGCG AGTTGATGCT TCAGATGAGG ACCGCATTTC GGAAGTACGA 
AAAGTTTTGA ACTCGGGAAA CTTTTATTTT GCATGGTCTG CATCTGGAGT CAGCTTAGAT 
CTGAGTCTGA ATGCACATCG AAGCATGCAA GAGCACACAA CCGACAATAG GTTTTTCTGG 
AATCAGTCTC TGCATTTGCA TCTCAAGCAC TATGGTGTGA ACTGCGATGA CTGGTTATTA 
CGGCTCATGT GTGGGGGAGT AGAGATTAGA ACCATTTATG CTGCCCATAA ACAGGCAAAA 
GCTTGCCTCA TCTCAAGACT AAGCTGTGAA CGAGCTGGGA CAAGGTTCAA TGTCCGGGGA 
ACCAACGATG ATGGTCATGT TGCCAACTTT GTAGAGACAG AGCAGGTTAT ATACTTAGAT 
GACTGTGTCT CTTCCTTCAT ACAAATCCGA GGATCTGTTC CATTGTTCTG GGAGCAACCA 
GGGTTGCAAG TGGGATCCCA TCGTGTTCGT ATGTCAAGGG GATTTGAAGC CAATGCGCCT 
GCCTTTGACA GGCATTTCCG AACACTTAAG GACTTATATG GTAAACAGAT AGTAGTGAAT 
CTGCTTGGGT CTAAGGAAGG CGAGCATATG CTGAGCAAGG CTTTCCAGAG TCATTTAAAA 
GCCTCTGAAC ACGCTTCTGA TATCCACATG GTGTCTTTTG ACTATCATCA AATGGTTAAA 
GGAGGAAAGG CAGAAAAACT ACACAGTATT CTCAAACCAC AAGTCCAGAA GTTTCTAGAT 
TATGGATTTT TTTATTTCGA TGGCAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA 
ACAAACTGCT TGGATTGTCT GGATAGAACA AATAGTGTGC AGGCATTCCT TGGCTTAGAG 
ATGCTCGCTA AGCAGCTGGA AGCTCTGGGC TTGGCTGAGA AGCCTCAGCT GGTGACCCGC 
TTCCAAGAAG TTTTCCGGTC CATGTGGTCT GTGAATGGCG ACTCAATCAG TAAAATATAT 
GCAGGCACCG GGGCCCTGGA AGGGAAGGCC AAGTTAAAAG ATGGTGCGCG ATCTGTCACC 
AGAACCATCC AGAATAACTT CTTCGACAGC TCCAAGCAGG AAGCCATTGA TGTCCTGCTC 
CTGGGAAATA CTCTCAACAG CGATTTAGCT GACAAAGCCC GAGCGCTTCT AACTACTGGA 
AGTTTGCGTG TTTCTGAACA GACATTACAG TCAGCATCTT CCAAAGTCTT AAAGAACATG 
TGTGAGAACT TCTACAAATA CTCAAAGCCC AAGAAGATCC GAGTGTGTGT TGGCACCTGG 
AATGTGAACG GCGGGAAGCA GTTCCGCAGC ATAGCGTTCA AGAACCAGAC GCTCACAGAC 
TGGCTTCTGG ACGCTCCCAA GTTAGCCGGC ATCCAGGAGT TTCAAGATAA GAGAAGTAAG 
CCAACTGATA TATTTGCAAT TGGCTTTGAA GAAATGGTGG AGCTGAATGC TGGGAACATT 
GTGAATGCAA GCACAACCAA CCAGAAGCTG TGGGCAGTGG AGCTGCAGAA GACCATCTCC 
AGGGACAACA AGTATGTCCT GCTGGCCTCG GAGCAGCTGG TGGGTGTCTG CCTGTTTGTC 
TTTATCAGAC CACAGCACGC TCCTTTTATC AGGGATGTTG CAGTTGATAC TGTGAAAACT 
GGCATGGGAG GTGCAACTGG AAACAAGGGC GCCGTTGCAA TTCGGATGCT CTTCCACACC 
ACCAGCCTTT GCTTCGTCTG CAGCCACTTT GCTGCAGGGC AGTCCCAAGT CAAAGAGAGA 
AATGAAGATT TTGTAGAAAT AGCACGAAAA CTGAGTTTCC CCATGGGACG AATGCTCTTC 
TCCCATGACT ATGTATTTTG GTGTGGTGAT TTCAACTATC GAATTGACCT TCCGAATGAA 
GAGGTTAAAG AGCTCATAAG ACAACAAAAC TGGGATTCTC TGATCGCTGG GGATCAGCTC 
ATCAACCAGA AAAATGCAGG ACAGATCTTT AGAGGATTCT TAGAGGGAAA AGTAACGTTT 
GCTCCAACCT ATAAATATGA CTTGTTTTCT GAAGACTATG ACACCAGTGA GAAGTGTCGC 
ACCCCTGCTT GGACAGACCG TGTCCTCTGG AGAAGGAGGA AGTGGCCTTT TGACAGATCA 
GCTGAAGATT TAGATCTCCT GAATGCTAGT TTCCAAGATG AAAGTAAAAT TCTTTATACA 
TGGACCCCTG GCACCTTGCT GCACTATGGA AGAGCCGAGC TGAAGACTTC TGACCATAGG 
CCCGTCGTTG CCTTGATTGA CATAGATATA TTTGAAGTTG AAGCTGAAGA GAGACAAAAA 
ATTTATAAAG AAGTTATTGC CGTCCAGGGC CCACCAGATG GCACAGTGCT GGTCTCAATC 
AAAAGTTCTG CACAAGAAAG TACTTTTTTT GATGATGCTT TGATTGATGA GCTTCTGCGG 
CAGTTTGCAC ACTTTGGTGA AGTTATACTC ATAAGATTTG TAGAAGATAA AATGTGGGTT 
ACATTTTTGG AGGGAAGCTC TGCCTTGAAT GCTCTGAGCC TAAATGGGAA AGAGCTATTA 
AATCGGACTA TAACAATTAC TTTAAAAAGT CCAGACTGGA TCAAACATTT GGAAGAAGAG 
ATGAGTTTAG AGAAAATCAG TGTCACGTTG CCCTCATCAG CAAGCTCCAC CCTGCTTGGT 
GAAGACGCAG AGGTCGCAGC AGACTTTGAC ATGGAAGGTG ACGTCGATGA CTACAGCGCT 
GAAGTAGAGG AGCTTCTTCC TCAGCATCTG CAGCCGTCCT CAAGTTCTGG CCTGGGCACT 
TCTCCAAGTT CTTCACCCCG GACCAGTCCC TGCCAGTCCC CCACAGTACC AGAGTACTCC 
GCCCCTTCTC TTCCCATCAG ACCTAGCCGA GCACCGTCAA GAACTCCAGG ACCTCCAAGT 
TCGCAGGGCT CTCCAGTTGA CACTCAGCCA GCGGCCCAGA AGGATTCTTC CCAGACTCTA 
GAGCCCAAGA GACCACCCCC TCCCCGCCCA GTCGCTCCTC CGGCACGTCC TGCCCCACCA 
CAGAGACCAC CTCCACCTTC AGGGGCTAGG AGTCCTGCGC CTGCTAGAAA AGAATTTGGA 
GGTGTTGGAG CCCCTCCCAG TCCCGGGGTT GCTAGGAGAG AGATAGAAGC ACCCAAAAGC 
CCTGGAACAG CACGGAAAGA TAATATAGGG CGTAATCAGC CTTCCCCTCA AGCCGGACTT 
GCAGGCCCAG GACCCGCTGG ATACGGTGCG GCTAGACCGA CAATTCCAGC TCGTGCTGGA 
GTGATCAGCG CCCCTCAGAG CCAGGCTCGG GTATGTGCTG GAAGGCCGAC TCCTGACAGC 
CAAAGCAAGC CCTCGGAGAC ATTGAAAGGT CCTGCTGTCC TTCCAGAACC ACTGAAGCCT 
CAGGCTGCAT TTCCTCAGCA GCCTTCTCTG CCCACACCTG CTCAAAAGTT ACAGGACCCG 
CTTGTCCCCA TAGCAGCACC TACTATGCCT CCCTCTGGCC CCCAACCAAA TTTGGAAACC 
CCCCCACAGC CCCCACCTCG GAGCAGGTCA TCTCAAAGCT TGCCTTCAGA CTCCTCACCA 
CAGCTGCAGG TAAAAATAAA TGGCATTTCT GGTGTCAAAC AAGAGCCAAC ATTGAAGAGT 
GACCCATTTG AAGATCTCTC ACTTAGTGTG CTTGCTGTAT CAAAGGCTCA GCCTTCTGTT 
CAGATTTCAC CTGTTCTAAC CCCAGACCCA AAGATGTTGA TTCAGTTGCC TTCTGCATCG 
CAAAGTCAAG TTAACCCTTT GAGTTCTGTA AGTTGCATGC CAACCAGGCC TCCAGGTCCA 
GAGGAAAGCA AGTCACAGGA GAGTATGGGC AGTTCTGCAA ACCCGTTTCC CAGCCTGCCC 
TGCCGGAACC CTTTTACAGA CAGGACTGCT GCCCCTGGAA ACCCATTTAG AGTTCAGTCC 
CAAGAATCAG AGGCAACTTC TTGGCTCTCC AAAGAAGAGC CTGTTCCCAA CAGTCCCTTC 
CCTCCTCTCA TGCCTCTCAG TCATGACACG AGCAAGGCTT CAAGTTCGCT TGGTGGCTTT 
GAGGACAATT TTGATCTGCA GAGCCAGTCT ACAGTAAAAA CAAGCAACCC CAAAGGATGG 
GTAACCTTTG ACGAAGACGA CAACTTTCCT ACAACAGGAA AGTCAAAGTC AGTTTGTCCA 
GACTTAGTGG GTAACGCGCC AGCTTCATTT GATGATGACT GGAGTAAAGG TGCGAGCGTT 
TCTTTCTGTG TGTTGCCAGC CAGAAGGCCA CCACCACCAC CACCTCCTGT CCCTCTGCTA 
CCACCTGGCA CAACCTCATC TGCAGGACCT AGCACAACCC TGCCATCTAA GGCACCTTCC 
ACATTGGACT TCACGGAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_006522837.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522900.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X5, mRNA.

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CloneID	OMu52615
Clone ID Related Accession (Same CDS sequence)	XM_006522837.3 , XM_006522837.4
Accession Version	XM_006522837.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522837.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGCACCCAA AAGCCCTGGA ACAGCACGGA AAGATAATAT AGGGCGTAAT 
CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC CCAGGACCCG CTGGATACGG TGCGGCTAGA 
CCGACAATTC CAGCTCGTGC TGGAGTGATC AGCGCCCCTC AGAGCCAGGC TCGGGTATGT 
GCTGGAAGGC CGACTCCTGA CAGCCAAAGC AAGCCCTCGG AGACATTGAA AGGTCCTGCT 
GTCCTTCCAG AACCACTGAA GCCTCAGGCT GCATTTCCTC AGCAGCCTTC TCTGCCCACA 
CCTGCTCAAA AGTTACAGGA CCCGCTTGTC CCCATAGCAG CACCTACTAT GCCTCCCTCT 
GGCCCCCAAC CAAATTTGGA AACCCCCCCA CAGCCCCCAC CTCGGAGCAG GTCATCTCAA 
AGCTTGCCTT CAGACTCCTC ACCACAGCTG CAGGTAAAAA TAAATGGCAT TTCTGGTGTC 
AAACAAGAGC CAACATTGAA GAGTGACCCA TTTGAAGATC TCTCACTTAG TGTGCTTGCT 
GTATCAAAGG CTCAGCCTTC TGTTCAGATT TCACCTGTTC TAACCCCAGA CCCAAAGATG 
TTGATTCAGT TGCCTTCTGC ATCGCAAAGT CAAGTTAACC CTTTGAGTTC TGTAAGTTGC 
ATGCCAACCA GGCCTCCAGG TCCAGAGGAA AGCAAGTCAC AGGAGAGTAT GGGCAGTTCT 
GCAAACCCGT TTCCCAGCCT GCCCTGCCGG AACCCTTTTA CAGACAGGAC TGCTGCCCCT 
GGAAACCCAT TTAGAGTTCA GTCCCAAGAA TCAGAGGCAA CTTCTTGGCT CTCCAAAGAA 
GAGCCTGTTC CCAACAGTCC CTTCCCTCCT CTCATGCCTC TCAGTCATGA CACGAGCAAG 
GCTTCAAGTT CGCTTGGTGG CTTTGAGGAC AATTTTGATC TGCAGAGCCA GTCTACAGTA 
AAAACAAGCA ACCCCAAAGG ATGGGTAACC TTTGACGAAG ACGACAACTT TCCTACAACA 
GGAAAGTCAA AGTCAGTTTG TCCAGACTTA GTGGGTAACG CGCCAGCTTC ATTTGATGAT 
GACTGGAGTA AAGGTGCGAG CGTTTCTTTC TGTGTGTTGC CAGCCAGAAG GCCACCACCA 
CCACCACCTC CTGTCCCTCT GCTACCACCT GGCACAACCT CATCTGCAGG ACCTAGCACA 
ACCCTGCCAT CTAAGGCACC TTCCACATTG GACTTCACGG AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522900.1
CDS	389..5476
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_006522837.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522837.3

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGCACCCAA AAGCCCTGGA ACAGCACGGA AAGATAATAT AGGGCGTAAT 
CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC CCAGGACCCG CTGGATACGG TGCGGCTAGA 
CCGACAATTC CAGCTCGTGC TGGAGTGATC AGCGCCCCTC AGAGCCAGGC TCGGGTATGT 
GCTGGAAGGC CGACTCCTGA CAGCCAAAGC AAGCCCTCGG AGACATTGAA AGGTCCTGCT 
GTCCTTCCAG AACCACTGAA GCCTCAGGCT GCATTTCCTC AGCAGCCTTC TCTGCCCACA 
CCTGCTCAAA AGTTACAGGA CCCGCTTGTC CCCATAGCAG CACCTACTAT GCCTCCCTCT 
GGCCCCCAAC CAAATTTGGA AACCCCCCCA CAGCCCCCAC CTCGGAGCAG GTCATCTCAA 
AGCTTGCCTT CAGACTCCTC ACCACAGCTG CAGGTAAAAA TAAATGGCAT TTCTGGTGTC 
AAACAAGAGC CAACATTGAA GAGTGACCCA TTTGAAGATC TCTCACTTAG TGTGCTTGCT 
GTATCAAAGG CTCAGCCTTC TGTTCAGATT TCACCTGTTC TAACCCCAGA CCCAAAGATG 
TTGATTCAGT TGCCTTCTGC ATCGCAAAGT CAAGTTAACC CTTTGAGTTC TGTAAGTTGC 
ATGCCAACCA GGCCTCCAGG TCCAGAGGAA AGCAAGTCAC AGGAGAGTAT GGGCAGTTCT 
GCAAACCCGT TTCCCAGCCT GCCCTGCCGG AACCCTTTTA CAGACAGGAC TGCTGCCCCT 
GGAAACCCAT TTAGAGTTCA GTCCCAAGAA TCAGAGGCAA CTTCTTGGCT CTCCAAAGAA 
GAGCCTGTTC CCAACAGTCC CTTCCCTCCT CTCATGCCTC TCAGTCATGA CACGAGCAAG 
GCTTCAAGTT CGCTTGGTGG CTTTGAGGAC AATTTTGATC TGCAGAGCCA GTCTACAGTA 
AAAACAAGCA ACCCCAAAGG ATGGGTAACC TTTGACGAAG ACGACAACTT TCCTACAACA 
GGAAAGTCAA AGTCAGTTTG TCCAGACTTA GTGGGTAACG CGCCAGCTTC ATTTGATGAT 
GACTGGAGTA AAGGTGCGAG CGTTTCTTTC TGTGTGTTGC CAGCCAGAAG GCCACCACCA 
CCACCACCTC CTGTCCCTCT GCTACCACCT GGCACAACCT CATCTGCAGG ACCTAGCACA 
ACCCTGCCAT CTAAGGCACC TTCCACATTG GACTTCACGG AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246075.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244377.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X1, mRNA.

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CloneID	OMu72067
Clone ID Related Accession (Same CDS sequence)	XM_011246075.2 , XM_011246075.3
Accession Version	XM_011246075.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246075.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG GTAAAAATAA ATGGCATTTC TGGTGTCAAA 
CAAGAGCCAA CATTGAAGAG TGACCCATTT GAAGATCTCT CACTTAGTGT GCTTGCTGTA 
TCAAAGGCTC AGCCTTCTGT TCAGATTTCA CCTGTTCTAA CCCCAGACCC AAAGATGTTG 
ATTCAGTTGC CTTCTGCATC GCAAAGTCAA GTTAACCCTT TGAGTTCTGT AAGTTGCATG 
CCAACCAGGC CTCCAGGTCC AGAGGAAAGC AAGTCACAGG AGAGTATGGG CAGTTCTGCA 
AACCCGTTTC CCAGCCTGCC CTGCCGGAAC CCTTTTACAG ACAGGACTGC TGCCCCTGGA 
AACCCATTTA GAGTTCAGTC CCAAGAATCA GAGGCAACTT CTTGGCTCTC CAAAGAAGAG 
CCTGTTCCCA ACAGTCCCTT CCCTCCTCTC ATGCCTCTCA GTCATGACAC GAGCAAGGCT 
TCAAGTTCGC TTGGTGGCTT TGAGGACAAT TTTGATCTGC AGAGCCAGTC TACAGTAAAA 
ACAAGCAACC CCAAAGGATG GGTAACCTTT GACGAAGACG ACAACTTTCC TACAACAGGA 
AAGTCAAAGT CAGTTTGTCC AGACTTAGTG GGTAACGCGC CAGCTTCATT TGATGATGAC 
TGGAGTAAAG GTGCGAGCGT TTCTTTCTGT GTGTTGCCAG CCAGAAGGCC ACCACCACCA 
CCACCTCCTG TCCCTCTGCT ACCACCTGGC ACAACCTCAT CTGCAGGACC TAGCACAACC 
CTGCCATCTA AGGCACCTTC CACATTGGAC TTCACGGAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244377.1
CDS	393..5537
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246075.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246075.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG GTAAAAATAA ATGGCATTTC TGGTGTCAAA 
CAAGAGCCAA CATTGAAGAG TGACCCATTT GAAGATCTCT CACTTAGTGT GCTTGCTGTA 
TCAAAGGCTC AGCCTTCTGT TCAGATTTCA CCTGTTCTAA CCCCAGACCC AAAGATGTTG 
ATTCAGTTGC CTTCTGCATC GCAAAGTCAA GTTAACCCTT TGAGTTCTGT AAGTTGCATG 
CCAACCAGGC CTCCAGGTCC AGAGGAAAGC AAGTCACAGG AGAGTATGGG CAGTTCTGCA 
AACCCGTTTC CCAGCCTGCC CTGCCGGAAC CCTTTTACAG ACAGGACTGC TGCCCCTGGA 
AACCCATTTA GAGTTCAGTC CCAAGAATCA GAGGCAACTT CTTGGCTCTC CAAAGAAGAG 
CCTGTTCCCA ACAGTCCCTT CCCTCCTCTC ATGCCTCTCA GTCATGACAC GAGCAAGGCT 
TCAAGTTCGC TTGGTGGCTT TGAGGACAAT TTTGATCTGC AGAGCCAGTC TACAGTAAAA 
ACAAGCAACC CCAAAGGATG GGTAACCTTT GACGAAGACG ACAACTTTCC TACAACAGGA 
AAGTCAAAGT CAGTTTGTCC AGACTTAGTG GGTAACGCGC CAGCTTCATT TGATGATGAC 
TGGAGTAAAG GTGCGAGCGT TTCTTTCTGT GTGTTGCCAG CCAGAAGGCC ACCACCACCA 
CCACCTCCTG TCCCTCTGCT ACCACCTGGC ACAACCTCAT CTGCAGGACC TAGCACAACC 
CTGCCATCTA AGGCACCTTC CACATTGGAC TTCACGGAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246076.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244378.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X2, mRNA.

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CloneID	OMu72068
Clone ID Related Accession (Same CDS sequence)	XM_011246076.2 , XM_011246076.3
Accession Version	XM_011246076.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246076.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GCATCTTCCA AAGTCTTAAA GAACATGTGT 
GAGAACTTCT ACAAATACTC AAAGCCCAAG AAGATCCGAG TGTGTGTTGG CACCTGGAAT 
GTGAACGGCG GGAAGCAGTT CCGCAGCATA GCGTTCAAGA ACCAGACGCT CACAGACTGG 
CTTCTGGACG CTCCCAAGTT AGCCGGCATC CAGGAGTTTC AAGATAAGAG AAGTAAGCCA 
ACTGATATAT TTGCAATTGG CTTTGAAGAA ATGGTGGAGC TGAATGCTGG GAACATTGTG 
AATGCAAGCA CAACCAACCA GAAGCTGTGG GCAGTGGAGC TGCAGAAGAC CATCTCCAGG 
GACAACAAGT ATGTCCTGCT GGCCTCGGAG CAGCTGGTGG GTGTCTGCCT GTTTGTCTTT 
ATCAGACCAC AGCACGCTCC TTTTATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGC 
ATGGGAGGTG CAACTGGAAA CAAGGGCGCC GTTGCAATTC GGATGCTCTT CCACACCACC 
AGCCTTTGCT TCGTCTGCAG CCACTTTGCT GCAGGGCAGT CCCAAGTCAA AGAGAGAAAT 
GAAGATTTTG TAGAAATAGC ACGAAAACTG AGTTTCCCCA TGGGACGAAT GCTCTTCTCC 
CATGACTATG TATTTTGGTG TGGTGATTTC AACTATCGAA TTGACCTTCC GAATGAAGAG 
GTTAAAGAGC TCATAAGACA ACAAAACTGG GATTCTCTGA TCGCTGGGGA TCAGCTCATC 
AACCAGAAAA ATGCAGGACA GATCTTTAGA GGATTCTTAG AGGGAAAAGT AACGTTTGCT 
CCAACCTATA AATATGACTT GTTTTCTGAA GACTATGACA CCAGTGAGAA GTGTCGCACC 
CCTGCTTGGA CAGACCGTGT CCTCTGGAGA AGGAGGAAGT GGCCTTTTGA CAGATCAGCT 
GAAGATTTAG ATCTCCTGAA TGCTAGTTTC CAAGATGAAA GTAAAATTCT TTATACATGG 
ACCCCTGGCA CCTTGCTGCA CTATGGAAGA GCCGAGCTGA AGACTTCTGA CCATAGGCCC 
GTCGTTGCCT TGATTGACAT AGATATATTT GAAGTTGAAG CTGAAGAGAG ACAAAAAATT 
TATAAAGAAG TTATTGCCGT CCAGGGCCCA CCAGATGGCA CAGTGCTGGT CTCAATCAAA 
AGTTCTGCAC AAGAAAGTAC TTTTTTTGAT GATGCTTTGA TTGATGAGCT TCTGCGGCAG 
TTTGCACACT TTGGTGAAGT TATACTCATA AGATTTGTAG AAGATAAAAT GTGGGTTACA 
TTTTTGGAGG GAAGCTCTGC CTTGAATGCT CTGAGCCTAA ATGGGAAAGA GCTATTAAAT 
CGGACTATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AACATTTGGA AGAAGAGATG 
AGTTTAGAGA AAATCAGTGT CACGTTGCCC TCATCAGCAA GCTCCACCCT GCTTGGTGAA 
GACGCAGAGG TCGCAGCAGA CTTTGACATG GAAGGTGACG TCGATGACTA CAGCGCTGAA 
GTAGAGGAGC TTCTTCCTCA GCATCTGCAG CCGTCCTCAA GTTCTGGCCT GGGCACTTCT 
CCAAGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCCCCCA CAGTACCAGA GTACTCCGCC 
CCTTCTCTTC CCATCAGACC TAGCCGAGCA CCGTCAAGAA CTCCAGGACC TCCAAGTTCG 
CAGGGCTCTC CAGTTGACAC TCAGCCAGCG GCCCAGAAGG ATTCTTCCCA GACTCTAGAG 
CCCAAGAGAC CACCCCCTCC CCGCCCAGTC GCTCCTCCGG CACGTCCTGC CCCACCACAG 
AGACCACCTC CACCTTCAGG GGCTAGGAGT CCTGCGCCTG CTAGAAAAGA ATTTGGAGGT 
GTTGGAGCCC CTCCCAGTCC CGGGGTTGCT AGGAGAGAGA TAGAAGCACC CAAAAGCCCT 
GGAACAGCAC GGAAAGATAA TATAGGGCGT AATCAGCCTT CCCCTCAAGC CGGACTTGCA 
GGCCCAGGAC CCGCTGGATA CGGTGCGGCT AGACCGACAA TTCCAGCTCG TGCTGGAGTG 
ATCAGCGCCC CTCAGAGCCA GGCTCGGGTA TGTGCTGGAA GGCCGACTCC TGACAGCCAA 
AGCAAGCCCT CGGAGACATT GAAAGGTCCT GCTGTCCTTC CAGAACCACT GAAGCCTCAG 
GCTGCATTTC CTCAGCAGCC TTCTCTGCCC ACACCTGCTC AAAAGTTACA GGACCCGCTT 
GTCCCCATAG CAGCACCTAC TATGCCTCCC TCTGGCCCCC AACCAAATTT GGAAACCCCC 
CCACAGCCCC CACCTCGGAG CAGGTCATCT CAAAGCTTGC CTTCAGACTC CTCACCACAG 
CTGCAGGTAA AAATAAATGG CATTTCTGGT GTCAAACAAG AGCCAACATT GAAGAGTGAC 
CCATTTGAAG ATCTCTCACT TAGTGTGCTT GCTGTATCAA AGGCTCAGCC TTCTGTTCAG 
ATTTCACCTG TTCTAACCCC AGACCCAAAG ATGTTGATTC AGTTGCCTTC TGCATCGCAA 
AGTCAAGTTA ACCCTTTGAG TTCTGTAAGT TGCATGCCAA CCAGGCCTCC AGGTCCAGAG 
GAAAGCAAGT CACAGGAGAG TATGGGCAGT TCTGCAAACC CGTTTCCCAG CCTGCCCTGC 
CGGAACCCTT TTACAGACAG GACTGCTGCC CCTGGAAACC CATTTAGAGT TCAGTCCCAA 
GAATCAGAGG CAACTTCTTG GCTCTCCAAA GAAGAGCCTG TTCCCAACAG TCCCTTCCCT 
CCTCTCATGC CTCTCAGTCA TGACACGAGC AAGGCTTCAA GTTCGCTTGG TGGCTTTGAG 
GACAATTTTG ATCTGCAGAG CCAGTCTACA GTAAAAACAA GCAACCCCAA AGGATGGGTA 
ACCTTTGACG AAGACGACAA CTTTCCTACA ACAGGAAAGT CAAAGTCAGT TTGTCCAGAC 
TTAGTGGGTA ACGCGCCAGC TTCATTTGAT GATGACTGGA GTAAAGGTGC GAGCGTTTCT 
TTCTGTGTGT TGCCAGCCAG AAGGCCACCA CCACCACCAC CTCCTGTCCC TCTGCTACCA 
CCTGGCACAA CCTCATCTGC AGGACCTAGC ACAACCCTGC CATCTAAGGC ACCTTCCACA 
TTGGACTTCA CGGAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244378.1
CDS	520..5640
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246076.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246076.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GCATCTTCCA AAGTCTTAAA GAACATGTGT 
GAGAACTTCT ACAAATACTC AAAGCCCAAG AAGATCCGAG TGTGTGTTGG CACCTGGAAT 
GTGAACGGCG GGAAGCAGTT CCGCAGCATA GCGTTCAAGA ACCAGACGCT CACAGACTGG 
CTTCTGGACG CTCCCAAGTT AGCCGGCATC CAGGAGTTTC AAGATAAGAG AAGTAAGCCA 
ACTGATATAT TTGCAATTGG CTTTGAAGAA ATGGTGGAGC TGAATGCTGG GAACATTGTG 
AATGCAAGCA CAACCAACCA GAAGCTGTGG GCAGTGGAGC TGCAGAAGAC CATCTCCAGG 
GACAACAAGT ATGTCCTGCT GGCCTCGGAG CAGCTGGTGG GTGTCTGCCT GTTTGTCTTT 
ATCAGACCAC AGCACGCTCC TTTTATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGC 
ATGGGAGGTG CAACTGGAAA CAAGGGCGCC GTTGCAATTC GGATGCTCTT CCACACCACC 
AGCCTTTGCT TCGTCTGCAG CCACTTTGCT GCAGGGCAGT CCCAAGTCAA AGAGAGAAAT 
GAAGATTTTG TAGAAATAGC ACGAAAACTG AGTTTCCCCA TGGGACGAAT GCTCTTCTCC 
CATGACTATG TATTTTGGTG TGGTGATTTC AACTATCGAA TTGACCTTCC GAATGAAGAG 
GTTAAAGAGC TCATAAGACA ACAAAACTGG GATTCTCTGA TCGCTGGGGA TCAGCTCATC 
AACCAGAAAA ATGCAGGACA GATCTTTAGA GGATTCTTAG AGGGAAAAGT AACGTTTGCT 
CCAACCTATA AATATGACTT GTTTTCTGAA GACTATGACA CCAGTGAGAA GTGTCGCACC 
CCTGCTTGGA CAGACCGTGT CCTCTGGAGA AGGAGGAAGT GGCCTTTTGA CAGATCAGCT 
GAAGATTTAG ATCTCCTGAA TGCTAGTTTC CAAGATGAAA GTAAAATTCT TTATACATGG 
ACCCCTGGCA CCTTGCTGCA CTATGGAAGA GCCGAGCTGA AGACTTCTGA CCATAGGCCC 
GTCGTTGCCT TGATTGACAT AGATATATTT GAAGTTGAAG CTGAAGAGAG ACAAAAAATT 
TATAAAGAAG TTATTGCCGT CCAGGGCCCA CCAGATGGCA CAGTGCTGGT CTCAATCAAA 
AGTTCTGCAC AAGAAAGTAC TTTTTTTGAT GATGCTTTGA TTGATGAGCT TCTGCGGCAG 
TTTGCACACT TTGGTGAAGT TATACTCATA AGATTTGTAG AAGATAAAAT GTGGGTTACA 
TTTTTGGAGG GAAGCTCTGC CTTGAATGCT CTGAGCCTAA ATGGGAAAGA GCTATTAAAT 
CGGACTATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AACATTTGGA AGAAGAGATG 
AGTTTAGAGA AAATCAGTGT CACGTTGCCC TCATCAGCAA GCTCCACCCT GCTTGGTGAA 
GACGCAGAGG TCGCAGCAGA CTTTGACATG GAAGGTGACG TCGATGACTA CAGCGCTGAA 
GTAGAGGAGC TTCTTCCTCA GCATCTGCAG CCGTCCTCAA GTTCTGGCCT GGGCACTTCT 
CCAAGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCCCCCA CAGTACCAGA GTACTCCGCC 
CCTTCTCTTC CCATCAGACC TAGCCGAGCA CCGTCAAGAA CTCCAGGACC TCCAAGTTCG 
CAGGGCTCTC CAGTTGACAC TCAGCCAGCG GCCCAGAAGG ATTCTTCCCA GACTCTAGAG 
CCCAAGAGAC CACCCCCTCC CCGCCCAGTC GCTCCTCCGG CACGTCCTGC CCCACCACAG 
AGACCACCTC CACCTTCAGG GGCTAGGAGT CCTGCGCCTG CTAGAAAAGA ATTTGGAGGT 
GTTGGAGCCC CTCCCAGTCC CGGGGTTGCT AGGAGAGAGA TAGAAGCACC CAAAAGCCCT 
GGAACAGCAC GGAAAGATAA TATAGGGCGT AATCAGCCTT CCCCTCAAGC CGGACTTGCA 
GGCCCAGGAC CCGCTGGATA CGGTGCGGCT AGACCGACAA TTCCAGCTCG TGCTGGAGTG 
ATCAGCGCCC CTCAGAGCCA GGCTCGGGTA TGTGCTGGAA GGCCGACTCC TGACAGCCAA 
AGCAAGCCCT CGGAGACATT GAAAGGTCCT GCTGTCCTTC CAGAACCACT GAAGCCTCAG 
GCTGCATTTC CTCAGCAGCC TTCTCTGCCC ACACCTGCTC AAAAGTTACA GGACCCGCTT 
GTCCCCATAG CAGCACCTAC TATGCCTCCC TCTGGCCCCC AACCAAATTT GGAAACCCCC 
CCACAGCCCC CACCTCGGAG CAGGTCATCT CAAAGCTTGC CTTCAGACTC CTCACCACAG 
CTGCAGGTAA AAATAAATGG CATTTCTGGT GTCAAACAAG AGCCAACATT GAAGAGTGAC 
CCATTTGAAG ATCTCTCACT TAGTGTGCTT GCTGTATCAA AGGCTCAGCC TTCTGTTCAG 
ATTTCACCTG TTCTAACCCC AGACCCAAAG ATGTTGATTC AGTTGCCTTC TGCATCGCAA 
AGTCAAGTTA ACCCTTTGAG TTCTGTAAGT TGCATGCCAA CCAGGCCTCC AGGTCCAGAG 
GAAAGCAAGT CACAGGAGAG TATGGGCAGT TCTGCAAACC CGTTTCCCAG CCTGCCCTGC 
CGGAACCCTT TTACAGACAG GACTGCTGCC CCTGGAAACC CATTTAGAGT TCAGTCCCAA 
GAATCAGAGG CAACTTCTTG GCTCTCCAAA GAAGAGCCTG TTCCCAACAG TCCCTTCCCT 
CCTCTCATGC CTCTCAGTCA TGACACGAGC AAGGCTTCAA GTTCGCTTGG TGGCTTTGAG 
GACAATTTTG ATCTGCAGAG CCAGTCTACA GTAAAAACAA GCAACCCCAA AGGATGGGTA 
ACCTTTGACG AAGACGACAA CTTTCCTACA ACAGGAAAGT CAAAGTCAGT TTGTCCAGAC 
TTAGTGGGTA ACGCGCCAGC TTCATTTGAT GATGACTGGA GTAAAGGTGC GAGCGTTTCT 
TTCTGTGTGT TGCCAGCCAG AAGGCCACCA CCACCACCAC CTCCTGTCCC TCTGCTACCA 
CCTGGCACAA CCTCATCTGC AGGACCTAGC ACAACCCTGC CATCTAAGGC ACCTTCCACA 
TTGGACTTCA CGGAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246077.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244379.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X3, mRNA.

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CloneID	OMu72069
Clone ID Related Accession (Same CDS sequence)	XM_011246077.2 , XM_011246077.3
Accession Version	XM_011246077.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246077.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGTGTTGG AGCCCCTCCC 
AGTCCCGGGG TTGCTAGGAG AGAGATAGAA GCACCCAAAA GCCCTGGAAC AGCACGGAAA 
GATAATATAG GGCGTAATCA GCCTTCCCCT CAAGCCGGAC TTGCAGGCCC AGGACCCGCT 
GGATACGGTG CGGCTAGACC GACAATTCCA GCTCGTGCTG GAGTGATCAG CGCCCCTCAG 
AGCCAGGCTC GGGTATGTGC TGGAAGGCCG ACTCCTGACA GCCAAAGCAA GCCCTCGGAG 
ACATTGAAAG GTCCTGCTGT CCTTCCAGAA CCACTGAAGC CTCAGGCTGC ATTTCCTCAG 
CAGCCTTCTC TGCCCACACC TGCTCAAAAG TTACAGGACC CGCTTGTCCC CATAGCAGCA 
CCTACTATGC CTCCCTCTGG CCCCCAACCA AATTTGGAAA CCCCCCCACA GCCCCCACCT 
CGGAGCAGGT CATCTCAAAG CTTGCCTTCA GACTCCTCAC CACAGCTGCA GGTAAAAATA 
AATGGCATTT CTGGTGTCAA ACAAGAGCCA ACATTGAAGA GTGACCCATT TGAAGATCTC 
TCACTTAGTG TGCTTGCTGT ATCAAAGGCT CAGCCTTCTG TTCAGATTTC ACCTGTTCTA 
ACCCCAGACC CAAAGATGTT GATTCAGTTG CCTTCTGCAT CGCAAAGTCA AGTTAACCCT 
TTGAGTTCTG TAAGTTGCAT GCCAACCAGG CCTCCAGGTC CAGAGGAAAG CAAGTCACAG 
GAGAGTATGG GCAGTTCTGC AAACCCGTTT CCCAGCCTGC CCTGCCGGAA CCCTTTTACA 
GACAGGACTG CTGCCCCTGG AAACCCATTT AGAGTTCAGT CCCAAGAATC AGAGGCAACT 
TCTTGGCTCT CCAAAGAAGA GCCTGTTCCC AACAGTCCCT TCCCTCCTCT CATGCCTCTC 
AGTCATGACA CGAGCAAGGC TTCAAGTTCG CTTGGTGGCT TTGAGGACAA TTTTGATCTG 
CAGAGCCAGT CTACAGTAAA AACAAGCAAC CCCAAAGGAT GGGTAACCTT TGACGAAGAC 
GACAACTTTC CTACAACAGG AAAGTCAAAG TCAGTTTGTC CAGACTTAGT GGGTAACGCG 
CCAGCTTCAT TTGATGATGA CTGGAGTAAA GGTGCGAGCG TTTCTTTCTG TGTGTTGCCA 
GCCAGAAGGC CACCACCACC ACCACCTCCT GTCCCTCTGC TACCACCTGG CACAACCTCA 
TCTGCAGGAC CTAGCACAAC CCTGCCATCT AAGGCACCTT CCACATTGGA CTTCACGGAA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244379.1
CDS	393..5498
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246077.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246077.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGTGTTGG AGCCCCTCCC 
AGTCCCGGGG TTGCTAGGAG AGAGATAGAA GCACCCAAAA GCCCTGGAAC AGCACGGAAA 
GATAATATAG GGCGTAATCA GCCTTCCCCT CAAGCCGGAC TTGCAGGCCC AGGACCCGCT 
GGATACGGTG CGGCTAGACC GACAATTCCA GCTCGTGCTG GAGTGATCAG CGCCCCTCAG 
AGCCAGGCTC GGGTATGTGC TGGAAGGCCG ACTCCTGACA GCCAAAGCAA GCCCTCGGAG 
ACATTGAAAG GTCCTGCTGT CCTTCCAGAA CCACTGAAGC CTCAGGCTGC ATTTCCTCAG 
CAGCCTTCTC TGCCCACACC TGCTCAAAAG TTACAGGACC CGCTTGTCCC CATAGCAGCA 
CCTACTATGC CTCCCTCTGG CCCCCAACCA AATTTGGAAA CCCCCCCACA GCCCCCACCT 
CGGAGCAGGT CATCTCAAAG CTTGCCTTCA GACTCCTCAC CACAGCTGCA GGTAAAAATA 
AATGGCATTT CTGGTGTCAA ACAAGAGCCA ACATTGAAGA GTGACCCATT TGAAGATCTC 
TCACTTAGTG TGCTTGCTGT ATCAAAGGCT CAGCCTTCTG TTCAGATTTC ACCTGTTCTA 
ACCCCAGACC CAAAGATGTT GATTCAGTTG CCTTCTGCAT CGCAAAGTCA AGTTAACCCT 
TTGAGTTCTG TAAGTTGCAT GCCAACCAGG CCTCCAGGTC CAGAGGAAAG CAAGTCACAG 
GAGAGTATGG GCAGTTCTGC AAACCCGTTT CCCAGCCTGC CCTGCCGGAA CCCTTTTACA 
GACAGGACTG CTGCCCCTGG AAACCCATTT AGAGTTCAGT CCCAAGAATC AGAGGCAACT 
TCTTGGCTCT CCAAAGAAGA GCCTGTTCCC AACAGTCCCT TCCCTCCTCT CATGCCTCTC 
AGTCATGACA CGAGCAAGGC TTCAAGTTCG CTTGGTGGCT TTGAGGACAA TTTTGATCTG 
CAGAGCCAGT CTACAGTAAA AACAAGCAAC CCCAAAGGAT GGGTAACCTT TGACGAAGAC 
GACAACTTTC CTACAACAGG AAAGTCAAAG TCAGTTTGTC CAGACTTAGT GGGTAACGCG 
CCAGCTTCAT TTGATGATGA CTGGAGTAAA GGTGCGAGCG TTTCTTTCTG TGTGTTGCCA 
GCCAGAAGGC CACCACCACC ACCACCTCCT GTCCCTCTGC TACCACCTGG CACAACCTCA 
TCTGCAGGAC CTAGCACAAC CCTGCCATCT AAGGCACCTT CCACATTGGA CTTCACGGAA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246078.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244380.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X4, mRNA.

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CloneID	OMu72070
Clone ID Related Accession (Same CDS sequence)	XM_011246078.3 , XM_011246078.2
Accession Version	XM_011246078.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246078.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGCACCCAAA AGCCCTGGAA CAGCACGGAA AGATAATATA 
GGGCGTAATC AGCCTTCCCC TCAAGCCGGA CTTGCAGGCC CAGGACCCGC TGGATACGGT 
GCGGCTAGAC CGACAATTCC AGCTCGTGCT GGAGTGATCA GCGCCCCTCA GAGCCAGGCT 
CGGGTATGTG CTGGAAGGCC GACTCCTGAC AGCCAAAGCA AGCCCTCGGA GACATTGAAA 
GGTCCTGCTG TCCTTCCAGA ACCACTGAAG CCTCAGGCTG CATTTCCTCA GCAGCCTTCT 
CTGCCCACAC CTGCTCAAAA GTTACAGGAC CCGCTTGTCC CCATAGCAGC ACCTACTATG 
CCTCCCTCTG GCCCCCAACC AAATTTGGAA ACCCCCCCAC AGCCCCCACC TCGGAGCAGG 
TCATCTCAAA GCTTGCCTTC AGACTCCTCA CCACAGCTGC AGGTAAAAAT AAATGGCATT 
TCTGGTGTCA AACAAGAGCC AACATTGAAG AGTGACCCAT TTGAAGATCT CTCACTTAGT 
GTGCTTGCTG TATCAAAGGC TCAGCCTTCT GTTCAGATTT CACCTGTTCT AACCCCAGAC 
CCAAAGATGT TGATTCAGTT GCCTTCTGCA TCGCAAAGTC AAGTTAACCC TTTGAGTTCT 
GTAAGTTGCA TGCCAACCAG GCCTCCAGGT CCAGAGGAAA GCAAGTCACA GGAGAGTATG 
GGCAGTTCTG CAAACCCGTT TCCCAGCCTG CCCTGCCGGA ACCCTTTTAC AGACAGGACT 
GCTGCCCCTG GAAACCCATT TAGAGTTCAG TCCCAAGAAT CAGAGGCAAC TTCTTGGCTC 
TCCAAAGAAG AGCCTGTTCC CAACAGTCCC TTCCCTCCTC TCATGCCTCT CAGTCATGAC 
ACGAGCAAGG CTTCAAGTTC GCTTGGTGGC TTTGAGGACA ATTTTGATCT GCAGAGCCAG 
TCTACAGTAA AAACAAGCAA CCCCAAAGGA TGGGTAACCT TTGACGAAGA CGACAACTTT 
CCTACAACAG GAAAGTCAAA GTCAGTTTGT CCAGACTTAG TGGGTAACGC GCCAGCTTCA 
TTTGATGATG ACTGGAGTAA AGGTGCGAGC GTTTCTTTCT GTGTGTTGCC AGCCAGAAGG 
CCACCACCAC CACCACCTCC TGTCCCTCTG CTACCACCTG GCACAACCTC ATCTGCAGGA 
CCTAGCACAA CCCTGCCATC TAAGGCACCT TCCACATTGG ACTTCACGGA AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244380.1
CDS	392..5488
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246078.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246078.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGCACCCAAA AGCCCTGGAA CAGCACGGAA AGATAATATA 
GGGCGTAATC AGCCTTCCCC TCAAGCCGGA CTTGCAGGCC CAGGACCCGC TGGATACGGT 
GCGGCTAGAC CGACAATTCC AGCTCGTGCT GGAGTGATCA GCGCCCCTCA GAGCCAGGCT 
CGGGTATGTG CTGGAAGGCC GACTCCTGAC AGCCAAAGCA AGCCCTCGGA GACATTGAAA 
GGTCCTGCTG TCCTTCCAGA ACCACTGAAG CCTCAGGCTG CATTTCCTCA GCAGCCTTCT 
CTGCCCACAC CTGCTCAAAA GTTACAGGAC CCGCTTGTCC CCATAGCAGC ACCTACTATG 
CCTCCCTCTG GCCCCCAACC AAATTTGGAA ACCCCCCCAC AGCCCCCACC TCGGAGCAGG 
TCATCTCAAA GCTTGCCTTC AGACTCCTCA CCACAGCTGC AGGTAAAAAT AAATGGCATT 
TCTGGTGTCA AACAAGAGCC AACATTGAAG AGTGACCCAT TTGAAGATCT CTCACTTAGT 
GTGCTTGCTG TATCAAAGGC TCAGCCTTCT GTTCAGATTT CACCTGTTCT AACCCCAGAC 
CCAAAGATGT TGATTCAGTT GCCTTCTGCA TCGCAAAGTC AAGTTAACCC TTTGAGTTCT 
GTAAGTTGCA TGCCAACCAG GCCTCCAGGT CCAGAGGAAA GCAAGTCACA GGAGAGTATG 
GGCAGTTCTG CAAACCCGTT TCCCAGCCTG CCCTGCCGGA ACCCTTTTAC AGACAGGACT 
GCTGCCCCTG GAAACCCATT TAGAGTTCAG TCCCAAGAAT CAGAGGCAAC TTCTTGGCTC 
TCCAAAGAAG AGCCTGTTCC CAACAGTCCC TTCCCTCCTC TCATGCCTCT CAGTCATGAC 
ACGAGCAAGG CTTCAAGTTC GCTTGGTGGC TTTGAGGACA ATTTTGATCT GCAGAGCCAG 
TCTACAGTAA AAACAAGCAA CCCCAAAGGA TGGGTAACCT TTGACGAAGA CGACAACTTT 
CCTACAACAG GAAAGTCAAA GTCAGTTTGT CCAGACTTAG TGGGTAACGC GCCAGCTTCA 
TTTGATGATG ACTGGAGTAA AGGTGCGAGC GTTTCTTTCT GTGTGTTGCC AGCCAGAAGG 
CCACCACCAC CACCACCTCC TGTCCCTCTG CTACCACCTG GCACAACCTC ATCTGCAGGA 
CCTAGCACAA CCCTGCCATC TAAGGCACCT TCCACATTGG ACTTCACGGA AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246079.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244381.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X6, mRNA.

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CloneID	OMu72071
Clone ID Related Accession (Same CDS sequence)	XM_011246079.3 , XM_011246079.2
Accession Version	XM_011246079.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246079.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGCACCCAA AAGCCCTGGA 
ACAGCACGGA AAGATAATAT AGGGCGTAAT CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC 
CCAGGACCCG CTGGATACGG TGCGGCTAGA CCGACAATTC CAGCTCGTGC TGGAGTGATC 
AGCGCCCCTC AGAGCCAGGC TCGGGTATGT GCTGGAAGGC CGACTCCTGA CAGCCAAAGC 
AAGCCCTCGG AGACATTGAA AGGTCCTGCT GTCCTTCCAG AACCACTGAA GCCTCAGGCT 
GCATTTCCTC AGCAGCCTTC TCTGCCCACA CCTGCTCAAA AGTTACAGGA CCCGCTTGTC 
CCCATAGCAG CACCTACTAT GCCTCCCTCT GGCCCCCAAC CAAATTTGGA AACCCCCCCA 
CAGCCCCCAC CTCGGAGCAG GTCATCTCAA AGCTTGCCTT CAGACTCCTC ACCACAGCTG 
CAGGTAAAAA TAAATGGCAT TTCTGGTGTC AAACAAGAGC CAACATTGAA GAGTGACCCA 
TTTGAAGATC TCTCACTTAG TGTGCTTGCT GTATCAAAGG CTCAGCCTTC TGTTCAGATT 
TCACCTGTTC TAACCCCAGA CCCAAAGATG TTGATTCAGT TGCCTTCTGC ATCGCAAAGT 
CAAGTTAACC CTTTGAGTTC TGTAAGTTGC ATGCCAACCA GGCCTCCAGG TCCAGAGGAA 
AGCAAGTCAC AGGAGAGTAT GGGCAGTTCT GCAAACCCGT TTCCCAGCCT GCCCTGCCGG 
AACCCTTTTA CAGACAGGAC TGCTGCCCCT GGAAACCCAT TTAGAGTTCA GTCCCAAGAA 
TCAGAGGCAA CTTCTTGGCT CTCCAAAGAA GAGCCTGTTC CCAACAGTCC CTTCCCTCCT 
CTCATGCCTC TCAGTCATGA CACGAGCAAG GCTTCAAGTT CGCTTGGTGG CTTTGAGGAC 
AATTTTGATC TGCAGAGCCA GTCTACAGTA AAAACAAGCA ACCCCAAAGG ATGGGTAACC 
TTTGACGAAG ACGACAACTT TCCTACAACA GGAAAGTCAA AGTCAGTTTG TCCAGACTTA 
GTGGGTAACG CGCCAGCTTC ATTTGATGAT GACTGGAGTA AAGGTGCGAG CGTTTCTTTC 
TGTGTGTTGC CAGCCAGAAG GCCACCACCA CCACCACCTC CTGTCCCTCT GCTACCACCT 
GGCACAACCT CATCTGCAGG ACCTAGCACA ACCCTGCCAT CTAAGGCACC TTCCACATTG 
GACTTCACGG AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244381.1
CDS	390..5447
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246079.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246079.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGCACCCAA AAGCCCTGGA 
ACAGCACGGA AAGATAATAT AGGGCGTAAT CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC 
CCAGGACCCG CTGGATACGG TGCGGCTAGA CCGACAATTC CAGCTCGTGC TGGAGTGATC 
AGCGCCCCTC AGAGCCAGGC TCGGGTATGT GCTGGAAGGC CGACTCCTGA CAGCCAAAGC 
AAGCCCTCGG AGACATTGAA AGGTCCTGCT GTCCTTCCAG AACCACTGAA GCCTCAGGCT 
GCATTTCCTC AGCAGCCTTC TCTGCCCACA CCTGCTCAAA AGTTACAGGA CCCGCTTGTC 
CCCATAGCAG CACCTACTAT GCCTCCCTCT GGCCCCCAAC CAAATTTGGA AACCCCCCCA 
CAGCCCCCAC CTCGGAGCAG GTCATCTCAA AGCTTGCCTT CAGACTCCTC ACCACAGCTG 
CAGGTAAAAA TAAATGGCAT TTCTGGTGTC AAACAAGAGC CAACATTGAA GAGTGACCCA 
TTTGAAGATC TCTCACTTAG TGTGCTTGCT GTATCAAAGG CTCAGCCTTC TGTTCAGATT 
TCACCTGTTC TAACCCCAGA CCCAAAGATG TTGATTCAGT TGCCTTCTGC ATCGCAAAGT 
CAAGTTAACC CTTTGAGTTC TGTAAGTTGC ATGCCAACCA GGCCTCCAGG TCCAGAGGAA 
AGCAAGTCAC AGGAGAGTAT GGGCAGTTCT GCAAACCCGT TTCCCAGCCT GCCCTGCCGG 
AACCCTTTTA CAGACAGGAC TGCTGCCCCT GGAAACCCAT TTAGAGTTCA GTCCCAAGAA 
TCAGAGGCAA CTTCTTGGCT CTCCAAAGAA GAGCCTGTTC CCAACAGTCC CTTCCCTCCT 
CTCATGCCTC TCAGTCATGA CACGAGCAAG GCTTCAAGTT CGCTTGGTGG CTTTGAGGAC 
AATTTTGATC TGCAGAGCCA GTCTACAGTA AAAACAAGCA ACCCCAAAGG ATGGGTAACC 
TTTGACGAAG ACGACAACTT TCCTACAACA GGAAAGTCAA AGTCAGTTTG TCCAGACTTA 
GTGGGTAACG CGCCAGCTTC ATTTGATGAT GACTGGAGTA AAGGTGCGAG CGTTTCTTTC 
TGTGTGTTGC CAGCCAGAAG GCCACCACCA CCACCACCTC CTGTCCCTCT GCTACCACCT 
GGCACAACCT CATCTGCAGG ACCTAGCACA ACCCTGCCAT CTAAGGCACC TTCCACATTG 
GACTTCACGG AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246080.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244382.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu72072
Clone ID Related Accession (Same CDS sequence)	XM_011246080.2 , XM_011246080.3
Accession Version	XM_011246080.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGGCGTAAT CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC 
CCAGGACCCG CTGGATACGG TGCGGCTAGA CCGACAATTC CAGCTCGTGC TGGAGTGATC 
AGCGCCCCTC AGAGCCAGGC TCGGGTATGT GCTGGAAGGC CGACTCCTGA CAGCCAAAGC 
AAGCCCTCGG AGACATTGAA AGGTCCTGCT GTCCTTCCAG AACCACTGAA GCCTCAGGCT 
GCATTTCCTC AGCAGCCTTC TCTGCCCACA CCTGCTCAAA AGTTACAGGA CCCGCTTGTC 
CCCATAGCAG CACCTACTAT GCCTCCCTCT GGCCCCCAAC CAAATTTGGA AACCCCCCCA 
CAGCCCCCAC CTCGGAGCAG GTCATCTCAA AGCTTGCCTT CAGACTCCTC ACCACAGCTG 
CAGGTAAAAA TAAATGGCAT TTCTGGTGTC AAACAAGAGC CAACATTGAA GAGTGACCCA 
TTTGAAGATC TCTCACTTAG TGTGCTTGCT GTATCAAAGG CTCAGCCTTC TGTTCAGATT 
TCACCTGTTC TAACCCCAGA CCCAAAGATG TTGATTCAGT TGCCTTCTGC ATCGCAAAGT 
CAAGTTAACC CTTTGAGTTC TGTAAGTTGC ATGCCAACCA GGCCTCCAGG TCCAGAGGAA 
AGCAAGTCAC AGGAGAGTAT GGGCAGTTCT GCAAACCCGT TTCCCAGCCT GCCCTGCCGG 
AACCCTTTTA CAGACAGGAC TGCTGCCCCT GGAAACCCAT TTAGAGTTCA GTCCCAAGAA 
TCAGAGGCAA CTTCTTGGCT CTCCAAAGAA GAGCCTGTTC CCAACAGTCC CTTCCCTCCT 
CTCATGCCTC TCAGTCATGA CACGAGCAAG GCTTCAAGTT CGCTTGGTGG CTTTGAGGAC 
AATTTTGATC TGCAGAGCCA GTCTACAGTA AAAACAAGCA ACCCCAAAGG ATGGGTAACC 
TTTGACGAAG ACGACAACTT TCCTACAACA GGAAAGTCAA AGTCAGTTTG TCCAGACTTA 
GTGGGTAACG CGCCAGCTTC ATTTGATGAT GACTGGAGTA AAGGTGCGAG CGTTTCTTTC 
TGTGTGTTGC CAGCCAGAAG GCCACCACCA CCACCACCTC CTGTCCCTCT GCTACCACCT 
GGCACAACCT CATCTGCAGG ACCTAGCACA ACCCTGCCAT CTAAGGCACC TTCCACATTG 
GACTTCACGG AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244382.1
CDS	390..5447
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246080.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246080.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGGCGTAAT CAGCCTTCCC CTCAAGCCGG ACTTGCAGGC 
CCAGGACCCG CTGGATACGG TGCGGCTAGA CCGACAATTC CAGCTCGTGC TGGAGTGATC 
AGCGCCCCTC AGAGCCAGGC TCGGGTATGT GCTGGAAGGC CGACTCCTGA CAGCCAAAGC 
AAGCCCTCGG AGACATTGAA AGGTCCTGCT GTCCTTCCAG AACCACTGAA GCCTCAGGCT 
GCATTTCCTC AGCAGCCTTC TCTGCCCACA CCTGCTCAAA AGTTACAGGA CCCGCTTGTC 
CCCATAGCAG CACCTACTAT GCCTCCCTCT GGCCCCCAAC CAAATTTGGA AACCCCCCCA 
CAGCCCCCAC CTCGGAGCAG GTCATCTCAA AGCTTGCCTT CAGACTCCTC ACCACAGCTG 
CAGGTAAAAA TAAATGGCAT TTCTGGTGTC AAACAAGAGC CAACATTGAA GAGTGACCCA 
TTTGAAGATC TCTCACTTAG TGTGCTTGCT GTATCAAAGG CTCAGCCTTC TGTTCAGATT 
TCACCTGTTC TAACCCCAGA CCCAAAGATG TTGATTCAGT TGCCTTCTGC ATCGCAAAGT 
CAAGTTAACC CTTTGAGTTC TGTAAGTTGC ATGCCAACCA GGCCTCCAGG TCCAGAGGAA 
AGCAAGTCAC AGGAGAGTAT GGGCAGTTCT GCAAACCCGT TTCCCAGCCT GCCCTGCCGG 
AACCCTTTTA CAGACAGGAC TGCTGCCCCT GGAAACCCAT TTAGAGTTCA GTCCCAAGAA 
TCAGAGGCAA CTTCTTGGCT CTCCAAAGAA GAGCCTGTTC CCAACAGTCC CTTCCCTCCT 
CTCATGCCTC TCAGTCATGA CACGAGCAAG GCTTCAAGTT CGCTTGGTGG CTTTGAGGAC 
AATTTTGATC TGCAGAGCCA GTCTACAGTA AAAACAAGCA ACCCCAAAGG ATGGGTAACC 
TTTGACGAAG ACGACAACTT TCCTACAACA GGAAAGTCAA AGTCAGTTTG TCCAGACTTA 
GTGGGTAACG CGCCAGCTTC ATTTGATGAT GACTGGAGTA AAGGTGCGAG CGTTTCTTTC 
TGTGTGTTGC CAGCCAGAAG GCCACCACCA CCACCACCTC CTGTCCCTCT GCTACCACCT 
GGCACAACCT CATCTGCAGG ACCTAGCACA ACCCTGCCAT CTAAGGCACC TTCCACATTG 
GACTTCACGG AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246081.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244383.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X9, mRNA.

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CloneID	OMu72073
Clone ID Related Accession (Same CDS sequence)	XM_011246081.2 , XM_011246081.3
Accession Version	XM_011246081.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGCGTAA TCAGCCTTCC 
CCTCAAGCCG GACTTGCAGG CCCAGGACCC GCTGGATACG GTGCGGCTAG ACCGACAATT 
CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT CAGAGCCAGG CTCGGGTATG TGCTGGAAGG 
CCGACTCCTG ACAGCCAAAG CAAGCCCTCG GAGACATTGA AAGGTCCTGC TGTCCTTCCA 
GAACCACTGA AGCCTCAGGC TGCATTTCCT CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA 
AAGTTACAGG ACCCGCTTGT CCCCATAGCA GCACCTACTA TGCCTCCCTC TGGCCCCCAA 
CCAAATTTGG AAACCCCCCC ACAGCCCCCA CCTCGGAGCA GGTCATCTCA AAGCTTGCCT 
TCAGACTCCT CACCACAGCT GCAGGTAAAA ATAAATGGCA TTTCTGGTGT CAAACAAGAG 
CCAACATTGA AGAGTGACCC ATTTGAAGAT CTCTCACTTA GTGTGCTTGC TGTATCAAAG 
GCTCAGCCTT CTGTTCAGAT TTCACCTGTT CTAACCCCAG ACCCAAAGAT GTTGATTCAG 
TTGCCTTCTG CATCGCAAAG TCAAGTTAAC CCTTTGAGTT CTGTAAGTTG CATGCCAACC 
AGGCCTCCAG GTCCAGAGGA AAGCAAGTCA CAGGAGAGTA TGGGCAGTTC TGCAAACCCG 
TTTCCCAGCC TGCCCTGCCG GAACCCTTTT ACAGACAGGA CTGCTGCCCC TGGAAACCCA 
TTTAGAGTTC AGTCCCAAGA ATCAGAGGCA ACTTCTTGGC TCTCCAAAGA AGAGCCTGTT 
CCCAACAGTC CCTTCCCTCC TCTCATGCCT CTCAGTCATG ACACGAGCAA GGCTTCAAGT 
TCGCTTGGTG GCTTTGAGGA CAATTTTGAT CTGCAGAGCC AGTCTACAGT AAAAACAAGC 
AACCCCAAAG GATGGGTAAC CTTTGACGAA GACGACAACT TTCCTACAAC AGGAAAGTCA 
AAGTCAGTTT GTCCAGACTT AGTGGGTAAC GCGCCAGCTT CATTTGATGA TGACTGGAGT 
AAAGGTGCGA GCGTTTCTTT CTGTGTGTTG CCAGCCAGAA GGCCACCACC ACCACCACCT 
CCTGTCCCTC TGCTACCACC TGGCACAACC TCATCTGCAG GACCTAGCAC AACCCTGCCA 
TCTAAGGCAC CTTCCACATT GGACTTCACG GAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244383.1
CDS	375..5393
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246081.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246081.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGCGTAA TCAGCCTTCC 
CCTCAAGCCG GACTTGCAGG CCCAGGACCC GCTGGATACG GTGCGGCTAG ACCGACAATT 
CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT CAGAGCCAGG CTCGGGTATG TGCTGGAAGG 
CCGACTCCTG ACAGCCAAAG CAAGCCCTCG GAGACATTGA AAGGTCCTGC TGTCCTTCCA 
GAACCACTGA AGCCTCAGGC TGCATTTCCT CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA 
AAGTTACAGG ACCCGCTTGT CCCCATAGCA GCACCTACTA TGCCTCCCTC TGGCCCCCAA 
CCAAATTTGG AAACCCCCCC ACAGCCCCCA CCTCGGAGCA GGTCATCTCA AAGCTTGCCT 
TCAGACTCCT CACCACAGCT GCAGGTAAAA ATAAATGGCA TTTCTGGTGT CAAACAAGAG 
CCAACATTGA AGAGTGACCC ATTTGAAGAT CTCTCACTTA GTGTGCTTGC TGTATCAAAG 
GCTCAGCCTT CTGTTCAGAT TTCACCTGTT CTAACCCCAG ACCCAAAGAT GTTGATTCAG 
TTGCCTTCTG CATCGCAAAG TCAAGTTAAC CCTTTGAGTT CTGTAAGTTG CATGCCAACC 
AGGCCTCCAG GTCCAGAGGA AAGCAAGTCA CAGGAGAGTA TGGGCAGTTC TGCAAACCCG 
TTTCCCAGCC TGCCCTGCCG GAACCCTTTT ACAGACAGGA CTGCTGCCCC TGGAAACCCA 
TTTAGAGTTC AGTCCCAAGA ATCAGAGGCA ACTTCTTGGC TCTCCAAAGA AGAGCCTGTT 
CCCAACAGTC CCTTCCCTCC TCTCATGCCT CTCAGTCATG ACACGAGCAA GGCTTCAAGT 
TCGCTTGGTG GCTTTGAGGA CAATTTTGAT CTGCAGAGCC AGTCTACAGT AAAAACAAGC 
AACCCCAAAG GATGGGTAAC CTTTGACGAA GACGACAACT TTCCTACAAC AGGAAAGTCA 
AAGTCAGTTT GTCCAGACTT AGTGGGTAAC GCGCCAGCTT CATTTGATGA TGACTGGAGT 
AAAGGTGCGA GCGTTTCTTT CTGTGTGTTG CCAGCCAGAA GGCCACCACC ACCACCACCT 
CCTGTCCCTC TGCTACCACC TGGCACAACC TCATCTGCAG GACCTAGCAC AACCCTGCCA 
TCTAAGGCAC CTTCCACATT GGACTTCACG GAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246082.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244384.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X10, mRNA.

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CloneID	OMu72074
Clone ID Related Accession (Same CDS sequence)	XM_011246082.3 , XM_011246082.2
Accession Version	XM_011246082.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GCATCTTCCA AAGTCTTAAA GAACATGTGT 
GAGAACTTCT ACAAATACTC AAAGCCCAAG AAGATCCGAG TGTGTGTTGG CACCTGGAAT 
GTGAACGGCG GGAAGCAGTT CCGCAGCATA GCGTTCAAGA ACCAGACGCT CACAGACTGG 
CTTCTGGACG CTCCCAAGTT AGCCGGCATC CAGGAGTTTC AAGATAAGAG AAGTAAGCCA 
ACTGATATAT TTGCAATTGG CTTTGAAGAA ATGGTGGAGC TGAATGCTGG GAACATTGTG 
AATGCAAGCA CAACCAACCA GAAGCTGTGG GCAGTGGAGC TGCAGAAGAC CATCTCCAGG 
GACAACAAGT ATGTCCTGCT GGCCTCGGAG CAGCTGGTGG GTGTCTGCCT GTTTGTCTTT 
ATCAGACCAC AGCACGCTCC TTTTATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGC 
ATGGGAGGTG CAACTGGAAA CAAGGGCGCC GTTGCAATTC GGATGCTCTT CCACACCACC 
AGCCTTTGCT TCGTCTGCAG CCACTTTGCT GCAGGGCAGT CCCAAGTCAA AGAGAGAAAT 
GAAGATTTTG TAGAAATAGC ACGAAAACTG AGTTTCCCCA TGGGACGAAT GCTCTTCTCC 
CATGACTATG TATTTTGGTG TGGTGATTTC AACTATCGAA TTGACCTTCC GAATGAAGAG 
GTTAAAGAGC TCATAAGACA ACAAAACTGG GATTCTCTGA TCGCTGGGGA TCAGCTCATC 
AACCAGAAAA ATGCAGGACA GATCTTTAGA GGATTCTTAG AGGGAAAAGT AACGTTTGCT 
CCAACCTATA AATATGACTT GTTTTCTGAA GACTATGACA CCAGTGAGAA GTGTCGCACC 
CCTGCTTGGA CAGACCGTGT CCTCTGGAGA AGGAGGAAGT GGCCTTTTGA CAGATCAGCT 
GAAGATTTAG ATCTCCTGAA TGCTAGTTTC CAAGATGAAA GTAAAATTCT TTATACATGG 
ACCCCTGGCA CCTTGCTGCA CTATGGAAGA GCCGAGCTGA AGACTTCTGA CCATAGGCCC 
GTCGTTGCCT TGATTGACAT AGATATATTT GAAGTTGAAG CTGAAGAGAG ACAAAAAATT 
TATAAAGAAG TTATTGCCGT CCAGGGCCCA CCAGATGGCA CAGTGCTGGT CTCAATCAAA 
AGTTCTGCAC AAGAAAGTAC TTTTTTTGAT GATGCTTTGA TTGATGAGCT TCTGCGGCAG 
TTTGCACACT TTGGTGAAGT TATACTCATA AGATTTGTAG AAGATAAAAT GTGGGTTACA 
TTTTTGGAGG GAAGCTCTGC CTTGAATGCT CTGAGCCTAA ATGGGAAAGA GCTATTAAAT 
CGGACTATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AACATTTGGA AGAAGAGATG 
AGTTTAGAGA AAATCAGTGT CACGTTGCCC TCATCAGCAA GCTCCACCCT GCTTGGTGAA 
GACGCAGAGG TCGCAGCAGA CTTTGACATG GAAGGTGACG TCGATGACTA CAGCGCTGAA 
GTAGAGGAGC TTCTTCCTCA GCATCTGCAG CCGTCCTCAA GTTCTGGCCT GGGCACTTCT 
CCAAGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCCCCCA CAGTACCAGA GTACTCCGCC 
CCTTCTCTTC CCATCAGACC TAGCCGAGCA CCGTCAAGAA CTCCAGGACC TCCAAGTTCG 
CAGGGCTCTC CAGTTGACAC TCAGCCAGCG GCCCAGAAGG ATTCTTCCCA GACTCTAGAG 
CCCAAGAGAC CACCCCCTCC CCGCCCAGTC GCTCCTCCGG CACGTCCTGC CCCACCACAG 
AGACCACCTC CACCTTCAGG GCGTAATCAG CCTTCCCCTC AAGCCGGACT TGCAGGCCCA 
GGACCCGCTG GATACGGTGC GGCTAGACCG ACAATTCCAG CTCGTGCTGG AGTGATCAGC 
GCCCCTCAGA GCCAGGCTCG GGTATGTGCT GGAAGGCCGA CTCCTGACAG CCAAAGCAAG 
CCCTCGGAGA CATTGAAAGG TCCTGCTGTC CTTCCAGAAC CACTGAAGCC TCAGGCTGCA 
TTTCCTCAGC AGCCTTCTCT GCCCACACCT GCTCAAAAGT TACAGGACCC GCTTGTCCCC 
ATAGCAGCAC CTACTATGCC TCCCTCTGGC CCCCAACCAA ATTTGGAAAC CCCCCCACAG 
CCCCCACCTC GGAGCAGGTC ATCTCAAAGC TTGCCTTCAG ACTCCTCACC ACAGCTGCAG 
GTAAAAATAA ATGGCATTTC TGGTGTCAAA CAAGAGCCAA CATTGAAGAG TGACCCATTT 
GAAGATCTCT CACTTAGTGT GCTTGCTGTA TCAAAGGCTC AGCCTTCTGT TCAGATTTCA 
CCTGTTCTAA CCCCAGACCC AAAGATGTTG ATTCAGTTGC CTTCTGCATC GCAAAGTCAA 
GTTAACCCTT TGAGTTCTGT AAGTTGCATG CCAACCAGGC CTCCAGGTCC AGAGGAAAGC 
AAGTCACAGG AGAGTATGGG CAGTTCTGCA AACCCGTTTC CCAGCCTGCC CTGCCGGAAC 
CCTTTTACAG ACAGGACTGC TGCCCCTGGA AACCCATTTA GAGTTCAGTC CCAAGAATCA 
GAGGCAACTT CTTGGCTCTC CAAAGAAGAG CCTGTTCCCA ACAGTCCCTT CCCTCCTCTC 
ATGCCTCTCA GTCATGACAC GAGCAAGGCT TCAAGTTCGC TTGGTGGCTT TGAGGACAAT 
TTTGATCTGC AGAGCCAGTC TACAGTAAAA ACAAGCAACC CCAAAGGATG GGTAACCTTT 
GACGAAGACG ACAACTTTCC TACAACAGGA AAGTCAAAGT CAGTTTGTCC AGACTTAGTG 
GGTAACGCGC CAGCTTCATT TGATGATGAC TGGAGTAAAG GTGCGAGCGT TTCTTTCTGT 
GTGTTGCCAG CCAGAAGGCC ACCACCACCA CCACCTCCTG TCCCTCTGCT ACCACCTGGC 
ACAACCTCAT CTGCAGGACC TAGCACAACC CTGCCATCTA AGGCACCTTC CACATTGGAC 
TTCACGGAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244384.1
CDS	387..5381
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246082.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246082.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GCATCTTCCA AAGTCTTAAA GAACATGTGT 
GAGAACTTCT ACAAATACTC AAAGCCCAAG AAGATCCGAG TGTGTGTTGG CACCTGGAAT 
GTGAACGGCG GGAAGCAGTT CCGCAGCATA GCGTTCAAGA ACCAGACGCT CACAGACTGG 
CTTCTGGACG CTCCCAAGTT AGCCGGCATC CAGGAGTTTC AAGATAAGAG AAGTAAGCCA 
ACTGATATAT TTGCAATTGG CTTTGAAGAA ATGGTGGAGC TGAATGCTGG GAACATTGTG 
AATGCAAGCA CAACCAACCA GAAGCTGTGG GCAGTGGAGC TGCAGAAGAC CATCTCCAGG 
GACAACAAGT ATGTCCTGCT GGCCTCGGAG CAGCTGGTGG GTGTCTGCCT GTTTGTCTTT 
ATCAGACCAC AGCACGCTCC TTTTATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGC 
ATGGGAGGTG CAACTGGAAA CAAGGGCGCC GTTGCAATTC GGATGCTCTT CCACACCACC 
AGCCTTTGCT TCGTCTGCAG CCACTTTGCT GCAGGGCAGT CCCAAGTCAA AGAGAGAAAT 
GAAGATTTTG TAGAAATAGC ACGAAAACTG AGTTTCCCCA TGGGACGAAT GCTCTTCTCC 
CATGACTATG TATTTTGGTG TGGTGATTTC AACTATCGAA TTGACCTTCC GAATGAAGAG 
GTTAAAGAGC TCATAAGACA ACAAAACTGG GATTCTCTGA TCGCTGGGGA TCAGCTCATC 
AACCAGAAAA ATGCAGGACA GATCTTTAGA GGATTCTTAG AGGGAAAAGT AACGTTTGCT 
CCAACCTATA AATATGACTT GTTTTCTGAA GACTATGACA CCAGTGAGAA GTGTCGCACC 
CCTGCTTGGA CAGACCGTGT CCTCTGGAGA AGGAGGAAGT GGCCTTTTGA CAGATCAGCT 
GAAGATTTAG ATCTCCTGAA TGCTAGTTTC CAAGATGAAA GTAAAATTCT TTATACATGG 
ACCCCTGGCA CCTTGCTGCA CTATGGAAGA GCCGAGCTGA AGACTTCTGA CCATAGGCCC 
GTCGTTGCCT TGATTGACAT AGATATATTT GAAGTTGAAG CTGAAGAGAG ACAAAAAATT 
TATAAAGAAG TTATTGCCGT CCAGGGCCCA CCAGATGGCA CAGTGCTGGT CTCAATCAAA 
AGTTCTGCAC AAGAAAGTAC TTTTTTTGAT GATGCTTTGA TTGATGAGCT TCTGCGGCAG 
TTTGCACACT TTGGTGAAGT TATACTCATA AGATTTGTAG AAGATAAAAT GTGGGTTACA 
TTTTTGGAGG GAAGCTCTGC CTTGAATGCT CTGAGCCTAA ATGGGAAAGA GCTATTAAAT 
CGGACTATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AACATTTGGA AGAAGAGATG 
AGTTTAGAGA AAATCAGTGT CACGTTGCCC TCATCAGCAA GCTCCACCCT GCTTGGTGAA 
GACGCAGAGG TCGCAGCAGA CTTTGACATG GAAGGTGACG TCGATGACTA CAGCGCTGAA 
GTAGAGGAGC TTCTTCCTCA GCATCTGCAG CCGTCCTCAA GTTCTGGCCT GGGCACTTCT 
CCAAGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCCCCCA CAGTACCAGA GTACTCCGCC 
CCTTCTCTTC CCATCAGACC TAGCCGAGCA CCGTCAAGAA CTCCAGGACC TCCAAGTTCG 
CAGGGCTCTC CAGTTGACAC TCAGCCAGCG GCCCAGAAGG ATTCTTCCCA GACTCTAGAG 
CCCAAGAGAC CACCCCCTCC CCGCCCAGTC GCTCCTCCGG CACGTCCTGC CCCACCACAG 
AGACCACCTC CACCTTCAGG GCGTAATCAG CCTTCCCCTC AAGCCGGACT TGCAGGCCCA 
GGACCCGCTG GATACGGTGC GGCTAGACCG ACAATTCCAG CTCGTGCTGG AGTGATCAGC 
GCCCCTCAGA GCCAGGCTCG GGTATGTGCT GGAAGGCCGA CTCCTGACAG CCAAAGCAAG 
CCCTCGGAGA CATTGAAAGG TCCTGCTGTC CTTCCAGAAC CACTGAAGCC TCAGGCTGCA 
TTTCCTCAGC AGCCTTCTCT GCCCACACCT GCTCAAAAGT TACAGGACCC GCTTGTCCCC 
ATAGCAGCAC CTACTATGCC TCCCTCTGGC CCCCAACCAA ATTTGGAAAC CCCCCCACAG 
CCCCCACCTC GGAGCAGGTC ATCTCAAAGC TTGCCTTCAG ACTCCTCACC ACAGCTGCAG 
GTAAAAATAA ATGGCATTTC TGGTGTCAAA CAAGAGCCAA CATTGAAGAG TGACCCATTT 
GAAGATCTCT CACTTAGTGT GCTTGCTGTA TCAAAGGCTC AGCCTTCTGT TCAGATTTCA 
CCTGTTCTAA CCCCAGACCC AAAGATGTTG ATTCAGTTGC CTTCTGCATC GCAAAGTCAA 
GTTAACCCTT TGAGTTCTGT AAGTTGCATG CCAACCAGGC CTCCAGGTCC AGAGGAAAGC 
AAGTCACAGG AGAGTATGGG CAGTTCTGCA AACCCGTTTC CCAGCCTGCC CTGCCGGAAC 
CCTTTTACAG ACAGGACTGC TGCCCCTGGA AACCCATTTA GAGTTCAGTC CCAAGAATCA 
GAGGCAACTT CTTGGCTCTC CAAAGAAGAG CCTGTTCCCA ACAGTCCCTT CCCTCCTCTC 
ATGCCTCTCA GTCATGACAC GAGCAAGGCT TCAAGTTCGC TTGGTGGCTT TGAGGACAAT 
TTTGATCTGC AGAGCCAGTC TACAGTAAAA ACAAGCAACC CCAAAGGATG GGTAACCTTT 
GACGAAGACG ACAACTTTCC TACAACAGGA AAGTCAAAGT CAGTTTGTCC AGACTTAGTG 
GGTAACGCGC CAGCTTCATT TGATGATGAC TGGAGTAAAG GTGCGAGCGT TTCTTTCTGT 
GTGTTGCCAG CCAGAAGGCC ACCACCACCA CCACCTCCTG TCCCTCTGCT ACCACCTGGC 
ACAACCTCAT CTGCAGGACC TAGCACAACC CTGCCATCTA AGGCACCTTC CACATTGGAC 
TTCACGGAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246083.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244385.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X14, mRNA.

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CloneID	OMu72075
Clone ID Related Accession (Same CDS sequence)	XM_011246083.1
Accession Version	XM_011246083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTCA GCAAAGGATT TCGAATCTAC CACAAATTGG ATCCCCCACC GTTCAGCCTC 
ATAGTGGAAA CTAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTGGCTGTG 
CTCTCATCTG CAGAAAAGGA GGCTATTAAA GGCACATATG CCAAAGTACT GGATGCATAC 
GGACTTTTGG GAGTTTTACG ATTAAATCTT GGTGATACCA TGCTGCACTA TCTGGTCCTA 
GTCACTGGAT GCATGTCTGT GGGGAAAATC CAAGAATCTG AAGTTTTCCG AGTTACTTCC 
ACTGAGTTTA TATCATTGCG AGTTGATGCT TCAGATGAGG ACCGCATTTC GGAAGTACGA 
AAAGTTTTGA ACTCGGGAAA CTTTTATTTT GCATGGTCTG CATCTGGAGT CAGCTTAGAT 
CTGAGTCTGA ATGCACATCG AAGCATGCAA GAGCACACAA CCGACAATAG GTTTTTCTGG 
AATCAGTCTC TGCATTTGCA TCTCAAGCAC TATGGTGTGA ACTGCGATGA CTGGTTATTA 
CGGCTCATGT GTGGGGGAGT AGAGATTAGA ACCATTTATG CTGCCCATAA ACAGGCAAAA 
GCTTGCCTCA TCTCAAGACT AAGCTGTGAA CGAGCTGGGA CAAGGTTCAA TGTCCGGGGA 
ACCAACGATG ATGGTCATGT TGCCAACTTT GTAGAGACAG AGCAGGTTAT ATACTTAGAT 
GACTGTGTCT CTTCCTTCAT ACAAATCCGA GGATCTGTTC CATTGTTCTG GGAGCAACCA 
GGGTTGCAAG TGGGATCCCA TCGTGTTCGT ATGTCAAGGG GATTTGAAGC CAATGCGCCT 
GCCTTTGACA GGCATTTCCG AACACTTAAG GACTTATATG GTAAACAGAT AGTAGTGAAT 
CTGCTTGGGT CTAAGGAAGG CGAGCATATG CTGAGCAAGG CTTTCCAGAG TCATTTAAAA 
GCCTCTGAAC ACGCTTCTGA TATCCACATG GTGTCTTTTG ACTATCATCA AATGGTTAAA 
GGAGGAAAGG CAGAAAAACT ACACAGTATT CTCAAACCAC AAGTCCAGAA GTTTCTAGAT 
TATGGATTTT TTTATTTCGA TGGCAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA 
ACAAACTGCT TGGATTGTCT GGATAGAACA AATAGTGTGC AGGCATTCCT TGGCTTAGAG 
ATGCTCGCTA AGCAGCTGGA AGCTCTGGGC TTGGCTGAGA AGCCTCAGCT GGTGACCCGC 
TTCCAAGAAG TTTTCCGGTC CATGTGGTCT GTGAATGGCG ACTCAATCAG TAAAATATAT 
GCAGGCACCG GGGCCCTGGA AGGGAAGGCC AAGGCTGGAA AGTTAAAAGA TGGTGCGCGA 
TCTGTCACCA GAACCATCCA GAATAACTTC TTCGACAGCT CCAAGCAGGA AGCCATTGAT 
GTCCTGCTCC TGGGAAATAC TCTCAACAGC GATTTAGCTG ACAAAGCCCG AGCGCTTCTA 
ACTACTGGAA GTTTGCGTGT TTCTGAACAG ACATTACAGT CAGCATCTTC CAAAGTCTTA 
AAGAACATGT GTGAGAACTT CTACAAATAC TCAAAGCCCA AGAAGATCCG AGTGTGTGTT 
GGCACCTGGA ATGTGAACGG CGGGAAGCAG TTCCGCAGCA TAGCGTTCAA GAACCAGACG 
CTCACAGACT GGCTTCTGGA CGCTCCCAAG TTAGCCGGCA TCCAGGAGTT TCAAGATAAG 
AGAAGTAAGC CAACTGATAT ATTTGCAATT GGCTTTGAAG AAATGGTGGA GCTGAATGCT 
GGGAACATTG TGAATGCAAG CACAACCAAC CAGAAGCTGT GGGCAGTGGA GCTGCAGAAG 
ACCATCTCCA GGGACAACAA GTATGTCCTG CTGGCCTCGG AGCAGCTGGT GGGTGTCTGC 
CTGTTTGTCT TTATCAGACC ACAGCACGCT CCTTTTATCA GGGATGTTGC AGTTGATACT 
GTGAAAACTG GCATGGGAGG TGCAACTGGA AACAAGGGCG CCGTTGCAAT TCGGATGCTC 
TTCCACACCA CCAGCCTTTG CTTCGTCTGC AGCCACTTTG CTGCAGGGCA GTCCCAAGTC 
AAAGAGAGAA ATGAAGATTT TGTAGAAATA GCACGAAAAC TGAGTTTCCC CATGGGACGA 
ATGCTCTTCT CCCATGACTA TGTATTTTGG TGTGGTGATT TCAACTATCG AATTGACCTT 
CCGAATGAAG AGGTTAAAGA GCTCATAAGA CAACAAAACT GGGATTCTCT GATCGCTGGG 
GATCAGCTCA TCAACCAGAA AAATGCAGGA CAGATCTTTA GAGGATTCTT AGAGGGAAAA 
GTAACGTTTG CTCCAACCTA TAAATATGAC TTGTTTTCTG AAGACTATGA CACCAGTGAG 
AAGTGTCGCA CCCCTGCTTG GACAGACCGT GTCCTCTGGA GAAGGAGGAA GTGGCCTTTT 
GACAGATCAG CTGAAGATTT AGATCTCCTG AATGCTAGTT TCCAAGATGA AAGTAAAATT 
CTTTATACAT GGACCCCTGG CACCTTGCTG CACTATGGAA GAGCCGAGCT GAAGACTTCT 
GACCATAGGC CCGTCGTTGC CTTGATTGAC ATAGATATAT TTGAAGTTGA AGCTGAAGAG 
AGACAAAAAA TTTATAAAGA AGTTATTGCC GTCCAGGGCC CACCAGATGG CACAGTGCTG 
GTCTCAATCA AAAGTTCTGC ACAAGAAAGT ACTTTTTTTG ATGATGCTTT GATTGATGAG 
CTTCTGCGGC AGTTTGCACA CTTTGGTGAA GTTATACTCA TAAGATTTGT AGAAGATAAA 
ATGTGGGTTA CATTTTTGGA GGGAAGCTCT GCCTTGAATG CTCTGAGCCT AAATGGGAAA 
GAGCTATTAA ATCGGACTAT AACAATTACT TTAAAAAGTC CAGACTGGAT CAAACATTTG 
GAAGAAGAGA TGAGTTTAGA GAAAATCAGT GTCACGTTGC CCTCATCAGC AAGCTCCACC 
CTGCTTGGTG AAGACGCAGA GGTCGCAGCA GACTTTGACA TGGAAGGTGA CGTCGATGAC 
TACAGCGCTG AAGTAGAGGA GCTTCTTCCT CAGCATCTGC AGCCGTCCTC AAGTTCTGGC 
CTGGGCACTT CTCCAAGTTC TTCACCCCGG ACCAGTCCCT GCCAGTCCCC CACAGTACCA 
GAGTACTCCG CCCCTTCTCT TCCCATCAGA CCTAGCCGAG CACCGTCAAG AACTCCAGGA 
CCTCCAAGTT CGCAGGGCTC TCCAGTTGAC ACTCAGCCAG CGGCCCAGAA GGATTCTTCC 
CAGACTCTAG AGCCCAAGAG ACCACCCCCT CCCCGCCCAG TCGCTCCTCC GGCACGTCCT 
GCCCCACCAC AGAGACCACC TCCACCTTCA GGGGCTAGGA GTCCTGCGCC TGCTAGAAAA 
GAATTTGGAG GTGTTGGAGC CCCTCCCAGT CCCGGGGTTG CTAGGAGAGA GATAGAAGCA 
CCCAAAAGCC CTGGAACAGC ACGGAAAGAT AATATAGGGC GTAATCAGCC TTCCCCTCAA 
GCCGGACTTG CAGGCCCAGG ACCCGCTGGA TACGGTGCGG CTAGACCGAC AATTCCAGCT 
CGTGCTGGAG TGATCAGCGC CCCTCAGAGC CAGGCTCGGG TATGTGCTGG AAGGCCGACT 
CCTGACAGCC AAAGCAAGCC CTCGGAGACA TTGAAAGGTC CTGCTGTCCT TCCAGAACCA 
CTGAAGCCTC AGGCTGCATT TCCTCAGCAG CCTTCTCTGC CCACACCTGC TCAAAAGTTA 
CAGGACCCGC TTGTCCCCAT AGCAGCACCT ACTATGCCTC CCTCTGGCCC CCAACCAAAT 
TTGGAAACCC CCCCACAGCC CCCACCTCGG AGCAGGTCAT CTCAAAGCTT GCCTTCAGAC 
TCCTCACCAC AGCTGCAGGT AAAAATAAAT GGCATTTCTG GTGTCAAACA AGAGCCAACA 
TTGAAGAGTG ACCCATTTGA AGATCTCTCA CTTAGTGTGC TTGCTGTATC AAAGGCTCAG 
CCTTCTGTTC AGATTTCACC TGTTCTAACC CCAGACCCAA AGATGTTGAT TCAGTTGCCT 
TCTGCATCGC AAAGTCAAGT TAACCCTTTG AGTTCTGTAA GTTGCATGCC AACCAGGCCT 
CCAGGTCCAG AGGAAAGCAA GTCACAGGAG AGTATGGGCA GTTCTGCAAA CCCGTTTCCC 
AGCCTGCCCT GCCGGAACCC TTTTACAGAC AGGACTGCTG CCCCTGGAAA CCCATTTAGA 
GTTCAGTCCC AAGAATCAGA GGCAACTTCT TGGCTCTCCA AAGAAGAGCC TGTTCCCAAC 
AGTCCCTTCC CTCCTCTCAT GCCTCTCAGT CATGACACGA GCAAGGCTTC AAGTTCGCTT 
GGTGGCTTTG AGGACAATTT TGATCTGCAG AGCCAGTCTA CAGTAAAAAC AAGCAACCCC 
AAAGGATGGG TAACCTTTGA CGAAGACGAC AACTTTCCTA CAACAGGAAA GTCAAAGTCA 
GTTTGTCCAG ACTTAGTGGG TAACGCGCCA GCTTCATTTG ATGATGACTG GAGTAAAGGT 
GCGAGCGTTT CTTTCTGTGT GTTGCCAGCC AGAAGGCCAC CACCACCACC ACCTCCTGTC 
CCTCTGCTAC CACCTGGCAC AACCTCATCT GCAGGACCTA GCACAACCCT GCCATCTAAG 
GCACCTTCCA CATTGGACTT CACGGAAAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244385.1
CDS	146..4858
Translation	MAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246083.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246083.1

ORF Insert Sequence:

ATGGCGTTCA GCAAAGGATT TCGAATCTAC CACAAATTGG ATCCCCCACC GTTCAGCCTC 
ATAGTGGAAA CTAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTGGCTGTG 
CTCTCATCTG CAGAAAAGGA GGCTATTAAA GGCACATATG CCAAAGTACT GGATGCATAC 
GGACTTTTGG GAGTTTTACG ATTAAATCTT GGTGATACCA TGCTGCACTA TCTGGTCCTA 
GTCACTGGAT GCATGTCTGT GGGGAAAATC CAAGAATCTG AAGTTTTCCG AGTTACTTCC 
ACTGAGTTTA TATCATTGCG AGTTGATGCT TCAGATGAGG ACCGCATTTC GGAAGTACGA 
AAAGTTTTGA ACTCGGGAAA CTTTTATTTT GCATGGTCTG CATCTGGAGT CAGCTTAGAT 
CTGAGTCTGA ATGCACATCG AAGCATGCAA GAGCACACAA CCGACAATAG GTTTTTCTGG 
AATCAGTCTC TGCATTTGCA TCTCAAGCAC TATGGTGTGA ACTGCGATGA CTGGTTATTA 
CGGCTCATGT GTGGGGGAGT AGAGATTAGA ACCATTTATG CTGCCCATAA ACAGGCAAAA 
GCTTGCCTCA TCTCAAGACT AAGCTGTGAA CGAGCTGGGA CAAGGTTCAA TGTCCGGGGA 
ACCAACGATG ATGGTCATGT TGCCAACTTT GTAGAGACAG AGCAGGTTAT ATACTTAGAT 
GACTGTGTCT CTTCCTTCAT ACAAATCCGA GGATCTGTTC CATTGTTCTG GGAGCAACCA 
GGGTTGCAAG TGGGATCCCA TCGTGTTCGT ATGTCAAGGG GATTTGAAGC CAATGCGCCT 
GCCTTTGACA GGCATTTCCG AACACTTAAG GACTTATATG GTAAACAGAT AGTAGTGAAT 
CTGCTTGGGT CTAAGGAAGG CGAGCATATG CTGAGCAAGG CTTTCCAGAG TCATTTAAAA 
GCCTCTGAAC ACGCTTCTGA TATCCACATG GTGTCTTTTG ACTATCATCA AATGGTTAAA 
GGAGGAAAGG CAGAAAAACT ACACAGTATT CTCAAACCAC AAGTCCAGAA GTTTCTAGAT 
TATGGATTTT TTTATTTCGA TGGCAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA 
ACAAACTGCT TGGATTGTCT GGATAGAACA AATAGTGTGC AGGCATTCCT TGGCTTAGAG 
ATGCTCGCTA AGCAGCTGGA AGCTCTGGGC TTGGCTGAGA AGCCTCAGCT GGTGACCCGC 
TTCCAAGAAG TTTTCCGGTC CATGTGGTCT GTGAATGGCG ACTCAATCAG TAAAATATAT 
GCAGGCACCG GGGCCCTGGA AGGGAAGGCC AAGGCTGGAA AGTTAAAAGA TGGTGCGCGA 
TCTGTCACCA GAACCATCCA GAATAACTTC TTCGACAGCT CCAAGCAGGA AGCCATTGAT 
GTCCTGCTCC TGGGAAATAC TCTCAACAGC GATTTAGCTG ACAAAGCCCG AGCGCTTCTA 
ACTACTGGAA GTTTGCGTGT TTCTGAACAG ACATTACAGT CAGCATCTTC CAAAGTCTTA 
AAGAACATGT GTGAGAACTT CTACAAATAC TCAAAGCCCA AGAAGATCCG AGTGTGTGTT 
GGCACCTGGA ATGTGAACGG CGGGAAGCAG TTCCGCAGCA TAGCGTTCAA GAACCAGACG 
CTCACAGACT GGCTTCTGGA CGCTCCCAAG TTAGCCGGCA TCCAGGAGTT TCAAGATAAG 
AGAAGTAAGC CAACTGATAT ATTTGCAATT GGCTTTGAAG AAATGGTGGA GCTGAATGCT 
GGGAACATTG TGAATGCAAG CACAACCAAC CAGAAGCTGT GGGCAGTGGA GCTGCAGAAG 
ACCATCTCCA GGGACAACAA GTATGTCCTG CTGGCCTCGG AGCAGCTGGT GGGTGTCTGC 
CTGTTTGTCT TTATCAGACC ACAGCACGCT CCTTTTATCA GGGATGTTGC AGTTGATACT 
GTGAAAACTG GCATGGGAGG TGCAACTGGA AACAAGGGCG CCGTTGCAAT TCGGATGCTC 
TTCCACACCA CCAGCCTTTG CTTCGTCTGC AGCCACTTTG CTGCAGGGCA GTCCCAAGTC 
AAAGAGAGAA ATGAAGATTT TGTAGAAATA GCACGAAAAC TGAGTTTCCC CATGGGACGA 
ATGCTCTTCT CCCATGACTA TGTATTTTGG TGTGGTGATT TCAACTATCG AATTGACCTT 
CCGAATGAAG AGGTTAAAGA GCTCATAAGA CAACAAAACT GGGATTCTCT GATCGCTGGG 
GATCAGCTCA TCAACCAGAA AAATGCAGGA CAGATCTTTA GAGGATTCTT AGAGGGAAAA 
GTAACGTTTG CTCCAACCTA TAAATATGAC TTGTTTTCTG AAGACTATGA CACCAGTGAG 
AAGTGTCGCA CCCCTGCTTG GACAGACCGT GTCCTCTGGA GAAGGAGGAA GTGGCCTTTT 
GACAGATCAG CTGAAGATTT AGATCTCCTG AATGCTAGTT TCCAAGATGA AAGTAAAATT 
CTTTATACAT GGACCCCTGG CACCTTGCTG CACTATGGAA GAGCCGAGCT GAAGACTTCT 
GACCATAGGC CCGTCGTTGC CTTGATTGAC ATAGATATAT TTGAAGTTGA AGCTGAAGAG 
AGACAAAAAA TTTATAAAGA AGTTATTGCC GTCCAGGGCC CACCAGATGG CACAGTGCTG 
GTCTCAATCA AAAGTTCTGC ACAAGAAAGT ACTTTTTTTG ATGATGCTTT GATTGATGAG 
CTTCTGCGGC AGTTTGCACA CTTTGGTGAA GTTATACTCA TAAGATTTGT AGAAGATAAA 
ATGTGGGTTA CATTTTTGGA GGGAAGCTCT GCCTTGAATG CTCTGAGCCT AAATGGGAAA 
GAGCTATTAA ATCGGACTAT AACAATTACT TTAAAAAGTC CAGACTGGAT CAAACATTTG 
GAAGAAGAGA TGAGTTTAGA GAAAATCAGT GTCACGTTGC CCTCATCAGC AAGCTCCACC 
CTGCTTGGTG AAGACGCAGA GGTCGCAGCA GACTTTGACA TGGAAGGTGA CGTCGATGAC 
TACAGCGCTG AAGTAGAGGA GCTTCTTCCT CAGCATCTGC AGCCGTCCTC AAGTTCTGGC 
CTGGGCACTT CTCCAAGTTC TTCACCCCGG ACCAGTCCCT GCCAGTCCCC CACAGTACCA 
GAGTACTCCG CCCCTTCTCT TCCCATCAGA CCTAGCCGAG CACCGTCAAG AACTCCAGGA 
CCTCCAAGTT CGCAGGGCTC TCCAGTTGAC ACTCAGCCAG CGGCCCAGAA GGATTCTTCC 
CAGACTCTAG AGCCCAAGAG ACCACCCCCT CCCCGCCCAG TCGCTCCTCC GGCACGTCCT 
GCCCCACCAC AGAGACCACC TCCACCTTCA GGGGCTAGGA GTCCTGCGCC TGCTAGAAAA 
GAATTTGGAG GTGTTGGAGC CCCTCCCAGT CCCGGGGTTG CTAGGAGAGA GATAGAAGCA 
CCCAAAAGCC CTGGAACAGC ACGGAAAGAT AATATAGGGC GTAATCAGCC TTCCCCTCAA 
GCCGGACTTG CAGGCCCAGG ACCCGCTGGA TACGGTGCGG CTAGACCGAC AATTCCAGCT 
CGTGCTGGAG TGATCAGCGC CCCTCAGAGC CAGGCTCGGG TATGTGCTGG AAGGCCGACT 
CCTGACAGCC AAAGCAAGCC CTCGGAGACA TTGAAAGGTC CTGCTGTCCT TCCAGAACCA 
CTGAAGCCTC AGGCTGCATT TCCTCAGCAG CCTTCTCTGC CCACACCTGC TCAAAAGTTA 
CAGGACCCGC TTGTCCCCAT AGCAGCACCT ACTATGCCTC CCTCTGGCCC CCAACCAAAT 
TTGGAAACCC CCCCACAGCC CCCACCTCGG AGCAGGTCAT CTCAAAGCTT GCCTTCAGAC 
TCCTCACCAC AGCTGCAGGT AAAAATAAAT GGCATTTCTG GTGTCAAACA AGAGCCAACA 
TTGAAGAGTG ACCCATTTGA AGATCTCTCA CTTAGTGTGC TTGCTGTATC AAAGGCTCAG 
CCTTCTGTTC AGATTTCACC TGTTCTAACC CCAGACCCAA AGATGTTGAT TCAGTTGCCT 
TCTGCATCGC AAAGTCAAGT TAACCCTTTG AGTTCTGTAA GTTGCATGCC AACCAGGCCT 
CCAGGTCCAG AGGAAAGCAA GTCACAGGAG AGTATGGGCA GTTCTGCAAA CCCGTTTCCC 
AGCCTGCCCT GCCGGAACCC TTTTACAGAC AGGACTGCTG CCCCTGGAAA CCCATTTAGA 
GTTCAGTCCC AAGAATCAGA GGCAACTTCT TGGCTCTCCA AAGAAGAGCC TGTTCCCAAC 
AGTCCCTTCC CTCCTCTCAT GCCTCTCAGT CATGACACGA GCAAGGCTTC AAGTTCGCTT 
GGTGGCTTTG AGGACAATTT TGATCTGCAG AGCCAGTCTA CAGTAAAAAC AAGCAACCCC 
AAAGGATGGG TAACCTTTGA CGAAGACGAC AACTTTCCTA CAACAGGAAA GTCAAAGTCA 
GTTTGTCCAG ACTTAGTGGG TAACGCGCCA GCTTCATTTG ATGATGACTG GAGTAAAGGT 
GCGAGCGTTT CTTTCTGTGT GTTGCCAGCC AGAAGGCCAC CACCACCACC ACCTCCTGTC 
CCTCTGCTAC CACCTGGCAC AACCTCATCT GCAGGACCTA GCACAACCCT GCCATCTAAG 
GCACCTTCCA CATTGGACTT CACGGAAAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246084.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244386.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X16, mRNA.

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CloneID	OMu72076
Clone ID Related Accession (Same CDS sequence)	XM_011246084.1
Accession Version	XM_011246084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTCA GCAAAGGATT TCGAATCTAC CACAAATTGG ATCCCCCACC GTTCAGCCTC 
ATAGTGGAAA CTAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTGGCTGTG 
CTCTCATCTG CAGAAAAGGA GGCTATTAAA GGCACATATG CCAAAGTACT GGATGCATAC 
GGACTTTTGG GAGTTTTACG ATTAAATCTT GGTGATACCA TGCTGCACTA TCTGGTCCTA 
GTCACTGGAT GCATGTCTGT GGGGAAAATC CAAGAATCTG AAGTTTTCCG AGTTACTTCC 
ACTGAGTTTA TATCATTGCG AGTTGATGCT TCAGATGAGG ACCGCATTTC GGAAGTACGA 
AAAGTTTTGA ACTCGGGAAA CTTTTATTTT GCATGGTCTG CATCTGGAGT CAGCTTAGAT 
CTGAGTCTGA ATGCACATCG AAGCATGCAA GAGCACACAA CCGACAATAG GTTTTTCTGG 
AATCAGTCTC TGCATTTGCA TCTCAAGCAC TATGGTGTGA ACTGCGATGA CTGGTTATTA 
CGGCTCATGT GTGGGGGAGT AGAGATTAGA ACCATTTATG CTGCCCATAA ACAGGCAAAA 
GCTTGCCTCA TCTCAAGACT AAGCTGTGAA CGAGCTGGGA CAAGGTTCAA TGTCCGGGGA 
ACCAACGATG ATGGTCATGT TGCCAACTTT GTAGAGACAG AGCAGGTTAT ATACTTAGAT 
GACTGTGTCT CTTCCTTCAT ACAAATCCGA GGATCTGTTC CATTGTTCTG GGAGCAACCA 
GGGTTGCAAG TGGGATCCCA TCGTGTTCGT ATGTCAAGGG GATTTGAAGC CAATGCGCCT 
GCCTTTGACA GGCATTTCCG AACACTTAAG GACTTATATG GTAAACAGAT AGTAGTGAAT 
CTGCTTGGGT CTAAGGAAGG CGAGCATATG CTGAGCAAGG CTTTCCAGAG TCATTTAAAA 
GCCTCTGAAC ACGCTTCTGA TATCCACATG GTGTCTTTTG ACTATCATCA AATGGTTAAA 
GGAGGAAAGG CAGAAAAACT ACACAGTATT CTCAAACCAC AAGTCCAGAA GTTTCTAGAT 
TATGGATTTT TTTATTTCGA TGGCAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA 
ACAAACTGCT TGGATTGTCT GGATAGAACA AATAGTGTGC AGGCATTCCT TGGCTTAGAG 
ATGCTCGCTA AGCAGCTGGA AGCTCTGGGC TTGGCTGAGA AGCCTCAGCT GGTGACCCGC 
TTCCAAGAAG TTTTCCGGTC CATGTGGTCT GTGAATGGCG ACTCAATCAG TAAAATATAT 
GCAGGCACCG GGGCCCTGGA AGGGAAGGCC AAGGCTGGAA AGTTAAAAGA TGGTGCGCGA 
TCTGTCACCA GAACCATCCA GAATAACTTC TTCGACAGCT CCAAGCAGGA AGCCATTGAT 
GTCCTGCTCC TGGGAAATAC TCTCAACAGC GATTTAGCTG ACAAAGCCCG AGCGCTTCTA 
ACTACTGGAA GTTTGCGTGC ATCTTCCAAA GTCTTAAAGA ACATGTGTGA GAACTTCTAC 
AAATACTCAA AGCCCAAGAA GATCCGAGTG TGTGTTGGCA CCTGGAATGT GAACGGCGGG 
AAGCAGTTCC GCAGCATAGC GTTCAAGAAC CAGACGCTCA CAGACTGGCT TCTGGACGCT 
CCCAAGTTAG CCGGCATCCA GGAGTTTCAA GATAAGAGAA GTAAGCCAAC TGATATATTT 
GCAATTGGCT TTGAAGAAAT GGTGGAGCTG AATGCTGGGA ACATTGTGAA TGCAAGCACA 
ACCAACCAGA AGCTGTGGGC AGTGGAGCTG CAGAAGACCA TCTCCAGGGA CAACAAGTAT 
GTCCTGCTGG CCTCGGAGCA GCTGGTGGGT GTCTGCCTGT TTGTCTTTAT CAGACCACAG 
CACGCTCCTT TTATCAGGGA TGTTGCAGTT GATACTGTGA AAACTGGCAT GGGAGGTGCA 
ACTGGAAACA AGGGCGCCGT TGCAATTCGG ATGCTCTTCC ACACCACCAG CCTTTGCTTC 
GTCTGCAGCC ACTTTGCTGC AGGGCAGTCC CAAGTCAAAG AGAGAAATGA AGATTTTGTA 
GAAATAGCAC GAAAACTGAG TTTCCCCATG GGACGAATGC TCTTCTCCCA TGACTATGTA 
TTTTGGTGTG GTGATTTCAA CTATCGAATT GACCTTCCGA ATGAAGAGGT TAAAGAGCTC 
ATAAGACAAC AAAACTGGGA TTCTCTGATC GCTGGGGATC AGCTCATCAA CCAGAAAAAT 
GCAGGACAGA TCTTTAGAGG ATTCTTAGAG GGAAAAGTAA CGTTTGCTCC AACCTATAAA 
TATGACTTGT TTTCTGAAGA CTATGACACC AGTGAGAAGT GTCGCACCCC TGCTTGGACA 
GACCGTGTCC TCTGGAGAAG GAGGAAGTGG CCTTTTGACA GATCAGCTGA AGATTTAGAT 
CTCCTGAATG CTAGTTTCCA AGATGAAAGT AAAATTCTTT ATACATGGAC CCCTGGCACC 
TTGCTGCACT ATGGAAGAGC CGAGCTGAAG ACTTCTGACC ATAGGCCCGT CGTTGCCTTG 
ATTGACATAG ATATATTTGA AGTTGAAGCT GAAGAGAGAC AAAAAATTTA TAAAGAAGTT 
ATTGCCGTCC AGGGCCCACC AGATGGCACA GTGCTGGTCT CAATCAAAAG TTCTGCACAA 
GAAAGTACTT TTTTTGATGA TGCTTTGATT GATGAGCTTC TGCGGCAGTT TGCACACTTT 
GGTGAAGTTA TACTCATAAG ATTTGTAGAA GATAAAATGT GGGTTACATT TTTGGAGGGA 
AGCTCTGCCT TGAATGCTCT GAGCCTAAAT GGGAAAGAGC TATTAAATCG GACTATAACA 
ATTACTTTAA AAAGTCCAGA CTGGATCAAA CATTTGGAAG AAGAGATGAG TTTAGAGAAA 
ATCAGTGTCA CGTTGCCCTC ATCAGCAAGC TCCACCCTGC TTGGTGAAGA CGCAGAGGTC 
GCAGCAGACT TTGACATGGA AGGTGACGTC GATGACTACA GCGCTGAAGT AGAGGAGCTT 
CTTCCTCAGC ATCTGCAGCC GTCCTCAAGT TCTGGCCTGG GCACTTCTCC AAGTTCTTCA 
CCCCGGACCA GTCCCTGCCA GTCCCCCACA GTACCAGAGT ACTCCGCCCC TTCTCTTCCC 
ATCAGACCTA GCCGAGCACC GTCAAGAACT CCAGGACCTC CAAGTTCGCA GGGCTCTCCA 
GTTGACACTC AGCCAGCGGC CCAGAAGGAT TCTTCCCAGA CTCTAGAGCC CAAGAGACCA 
CCCCCTCCCC GCCCAGTCGC TCCTCCGGCA CGTCCTGCCC CACCACAGAG ACCACCTCCA 
CCTTCAGGGG CTAGGAGTCC TGCGCCTGCT AGAAAAGAAT TTGGAGGTGT TGGAGCCCCT 
CCCAGTCCCG GGGTTGCTAG GAGAGAGATA GAAGCACCCA AAAGCCCTGG AACAGCACGG 
AAAGATAATA TAGGGCGTAA TCAGCCTTCC CCTCAAGCCG GACTTGCAGG CCCAGGACCC 
GCTGGATACG GTGCGGCTAG ACCGACAATT CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT 
CAGAGCCAGG CTCGGGTATG TGCTGGAAGG CCGACTCCTG ACAGCCAAAG CAAGCCCTCG 
GAGACATTGA AAGGTCCTGC TGTCCTTCCA GAACCACTGA AGCCTCAGGC TGCATTTCCT 
CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA AAGTTACAGG ACCCGCTTGT CCCCATAGCA 
GCACCTACTA TGCCTCCCTC TGGCCCCCAA CCAAATTTGG AAACCCCCCC ACAGCCCCCA 
CCTCGGAGCA GGTCATCTCA AAGCTTGCCT TCAGACTCCT CACCACAGCT GCAGGTAAAA 
ATAAATGGCA TTTCTGGTGT CAAACAAGAG CCAACATTGA AGAGTGACCC ATTTGAAGAT 
CTCTCACTTA GTGTGCTTGC TGTATCAAAG GCTCAGCCTT CTGTTCAGAT TTCACCTGTT 
CTAACCCCAG ACCCAAAGAT GTTGATTCAG TTGCCTTCTG CATCGCAAAG TCAAGTTAAC 
CCTTTGAGTT CTGTAAGTTG CATGCCAACC AGGCCTCCAG GTCCAGAGGA AAGCAAGTCA 
CAGGAGAGTA TGGGCAGTTC TGCAAACCCG TTTCCCAGCC TGCCCTGCCG GAACCCTTTT 
ACAGACAGGA CTGCTGCCCC TGGAAACCCA TTTAGAGTTC AGTCCCAAGA ATCAGAGGCA 
ACTTCTTGGC TCTCCAAAGA AGAGCCTGTT CCCAACAGTC CCTTCCCTCC TCTCATGCCT 
CTCAGTCATG ACACGAGCAA GGCTTCAAGT TCGCTTGGTG GCTTTGAGGA CAATTTTGAT 
CTGCAGAGCC AGTCTACAGT AAAAACAAGC AACCCCAAAG GATGGGTAAC CTTTGACGAA 
GACGACAACT TTCCTACAAC AGGAAAGTCA AAGTCAGTTT GTCCAGACTT AGTGGGTAAC 
GCGCCAGCTT CATTTGATGA TGACTGGAGT AAAGGTGCGA GCGTTTCTTT CTGTGTGTTG 
CCAGCCAGAA GGCCACCACC ACCACCACCT CCTGTCCCTC TGCTACCACC TGGCACAACC 
TCATCTGCAG GACCTAGCAC AACCCTGCCA TCTAAGGCAC CTTCCACATT GGACTTCACG 
GAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244386.1
CDS	146..4834
Translation	MAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQPSVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPFPSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKASSSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDDDWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER

Target ORF information:

RefSeq Version	XM_011246084.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246084.1

ORF Insert Sequence:

ATGGCGTTCA GCAAAGGATT TCGAATCTAC CACAAATTGG ATCCCCCACC GTTCAGCCTC 
ATAGTGGAAA CTAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTGGCTGTG 
CTCTCATCTG CAGAAAAGGA GGCTATTAAA GGCACATATG CCAAAGTACT GGATGCATAC 
GGACTTTTGG GAGTTTTACG ATTAAATCTT GGTGATACCA TGCTGCACTA TCTGGTCCTA 
GTCACTGGAT GCATGTCTGT GGGGAAAATC CAAGAATCTG AAGTTTTCCG AGTTACTTCC 
ACTGAGTTTA TATCATTGCG AGTTGATGCT TCAGATGAGG ACCGCATTTC GGAAGTACGA 
AAAGTTTTGA ACTCGGGAAA CTTTTATTTT GCATGGTCTG CATCTGGAGT CAGCTTAGAT 
CTGAGTCTGA ATGCACATCG AAGCATGCAA GAGCACACAA CCGACAATAG GTTTTTCTGG 
AATCAGTCTC TGCATTTGCA TCTCAAGCAC TATGGTGTGA ACTGCGATGA CTGGTTATTA 
CGGCTCATGT GTGGGGGAGT AGAGATTAGA ACCATTTATG CTGCCCATAA ACAGGCAAAA 
GCTTGCCTCA TCTCAAGACT AAGCTGTGAA CGAGCTGGGA CAAGGTTCAA TGTCCGGGGA 
ACCAACGATG ATGGTCATGT TGCCAACTTT GTAGAGACAG AGCAGGTTAT ATACTTAGAT 
GACTGTGTCT CTTCCTTCAT ACAAATCCGA GGATCTGTTC CATTGTTCTG GGAGCAACCA 
GGGTTGCAAG TGGGATCCCA TCGTGTTCGT ATGTCAAGGG GATTTGAAGC CAATGCGCCT 
GCCTTTGACA GGCATTTCCG AACACTTAAG GACTTATATG GTAAACAGAT AGTAGTGAAT 
CTGCTTGGGT CTAAGGAAGG CGAGCATATG CTGAGCAAGG CTTTCCAGAG TCATTTAAAA 
GCCTCTGAAC ACGCTTCTGA TATCCACATG GTGTCTTTTG ACTATCATCA AATGGTTAAA 
GGAGGAAAGG CAGAAAAACT ACACAGTATT CTCAAACCAC AAGTCCAGAA GTTTCTAGAT 
TATGGATTTT TTTATTTCGA TGGCAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA 
ACAAACTGCT TGGATTGTCT GGATAGAACA AATAGTGTGC AGGCATTCCT TGGCTTAGAG 
ATGCTCGCTA AGCAGCTGGA AGCTCTGGGC TTGGCTGAGA AGCCTCAGCT GGTGACCCGC 
TTCCAAGAAG TTTTCCGGTC CATGTGGTCT GTGAATGGCG ACTCAATCAG TAAAATATAT 
GCAGGCACCG GGGCCCTGGA AGGGAAGGCC AAGGCTGGAA AGTTAAAAGA TGGTGCGCGA 
TCTGTCACCA GAACCATCCA GAATAACTTC TTCGACAGCT CCAAGCAGGA AGCCATTGAT 
GTCCTGCTCC TGGGAAATAC TCTCAACAGC GATTTAGCTG ACAAAGCCCG AGCGCTTCTA 
ACTACTGGAA GTTTGCGTGC ATCTTCCAAA GTCTTAAAGA ACATGTGTGA GAACTTCTAC 
AAATACTCAA AGCCCAAGAA GATCCGAGTG TGTGTTGGCA CCTGGAATGT GAACGGCGGG 
AAGCAGTTCC GCAGCATAGC GTTCAAGAAC CAGACGCTCA CAGACTGGCT TCTGGACGCT 
CCCAAGTTAG CCGGCATCCA GGAGTTTCAA GATAAGAGAA GTAAGCCAAC TGATATATTT 
GCAATTGGCT TTGAAGAAAT GGTGGAGCTG AATGCTGGGA ACATTGTGAA TGCAAGCACA 
ACCAACCAGA AGCTGTGGGC AGTGGAGCTG CAGAAGACCA TCTCCAGGGA CAACAAGTAT 
GTCCTGCTGG CCTCGGAGCA GCTGGTGGGT GTCTGCCTGT TTGTCTTTAT CAGACCACAG 
CACGCTCCTT TTATCAGGGA TGTTGCAGTT GATACTGTGA AAACTGGCAT GGGAGGTGCA 
ACTGGAAACA AGGGCGCCGT TGCAATTCGG ATGCTCTTCC ACACCACCAG CCTTTGCTTC 
GTCTGCAGCC ACTTTGCTGC AGGGCAGTCC CAAGTCAAAG AGAGAAATGA AGATTTTGTA 
GAAATAGCAC GAAAACTGAG TTTCCCCATG GGACGAATGC TCTTCTCCCA TGACTATGTA 
TTTTGGTGTG GTGATTTCAA CTATCGAATT GACCTTCCGA ATGAAGAGGT TAAAGAGCTC 
ATAAGACAAC AAAACTGGGA TTCTCTGATC GCTGGGGATC AGCTCATCAA CCAGAAAAAT 
GCAGGACAGA TCTTTAGAGG ATTCTTAGAG GGAAAAGTAA CGTTTGCTCC AACCTATAAA 
TATGACTTGT TTTCTGAAGA CTATGACACC AGTGAGAAGT GTCGCACCCC TGCTTGGACA 
GACCGTGTCC TCTGGAGAAG GAGGAAGTGG CCTTTTGACA GATCAGCTGA AGATTTAGAT 
CTCCTGAATG CTAGTTTCCA AGATGAAAGT AAAATTCTTT ATACATGGAC CCCTGGCACC 
TTGCTGCACT ATGGAAGAGC CGAGCTGAAG ACTTCTGACC ATAGGCCCGT CGTTGCCTTG 
ATTGACATAG ATATATTTGA AGTTGAAGCT GAAGAGAGAC AAAAAATTTA TAAAGAAGTT 
ATTGCCGTCC AGGGCCCACC AGATGGCACA GTGCTGGTCT CAATCAAAAG TTCTGCACAA 
GAAAGTACTT TTTTTGATGA TGCTTTGATT GATGAGCTTC TGCGGCAGTT TGCACACTTT 
GGTGAAGTTA TACTCATAAG ATTTGTAGAA GATAAAATGT GGGTTACATT TTTGGAGGGA 
AGCTCTGCCT TGAATGCTCT GAGCCTAAAT GGGAAAGAGC TATTAAATCG GACTATAACA 
ATTACTTTAA AAAGTCCAGA CTGGATCAAA CATTTGGAAG AAGAGATGAG TTTAGAGAAA 
ATCAGTGTCA CGTTGCCCTC ATCAGCAAGC TCCACCCTGC TTGGTGAAGA CGCAGAGGTC 
GCAGCAGACT TTGACATGGA AGGTGACGTC GATGACTACA GCGCTGAAGT AGAGGAGCTT 
CTTCCTCAGC ATCTGCAGCC GTCCTCAAGT TCTGGCCTGG GCACTTCTCC AAGTTCTTCA 
CCCCGGACCA GTCCCTGCCA GTCCCCCACA GTACCAGAGT ACTCCGCCCC TTCTCTTCCC 
ATCAGACCTA GCCGAGCACC GTCAAGAACT CCAGGACCTC CAAGTTCGCA GGGCTCTCCA 
GTTGACACTC AGCCAGCGGC CCAGAAGGAT TCTTCCCAGA CTCTAGAGCC CAAGAGACCA 
CCCCCTCCCC GCCCAGTCGC TCCTCCGGCA CGTCCTGCCC CACCACAGAG ACCACCTCCA 
CCTTCAGGGG CTAGGAGTCC TGCGCCTGCT AGAAAAGAAT TTGGAGGTGT TGGAGCCCCT 
CCCAGTCCCG GGGTTGCTAG GAGAGAGATA GAAGCACCCA AAAGCCCTGG AACAGCACGG 
AAAGATAATA TAGGGCGTAA TCAGCCTTCC CCTCAAGCCG GACTTGCAGG CCCAGGACCC 
GCTGGATACG GTGCGGCTAG ACCGACAATT CCAGCTCGTG CTGGAGTGAT CAGCGCCCCT 
CAGAGCCAGG CTCGGGTATG TGCTGGAAGG CCGACTCCTG ACAGCCAAAG CAAGCCCTCG 
GAGACATTGA AAGGTCCTGC TGTCCTTCCA GAACCACTGA AGCCTCAGGC TGCATTTCCT 
CAGCAGCCTT CTCTGCCCAC ACCTGCTCAA AAGTTACAGG ACCCGCTTGT CCCCATAGCA 
GCACCTACTA TGCCTCCCTC TGGCCCCCAA CCAAATTTGG AAACCCCCCC ACAGCCCCCA 
CCTCGGAGCA GGTCATCTCA AAGCTTGCCT TCAGACTCCT CACCACAGCT GCAGGTAAAA 
ATAAATGGCA TTTCTGGTGT CAAACAAGAG CCAACATTGA AGAGTGACCC ATTTGAAGAT 
CTCTCACTTA GTGTGCTTGC TGTATCAAAG GCTCAGCCTT CTGTTCAGAT TTCACCTGTT 
CTAACCCCAG ACCCAAAGAT GTTGATTCAG TTGCCTTCTG CATCGCAAAG TCAAGTTAAC 
CCTTTGAGTT CTGTAAGTTG CATGCCAACC AGGCCTCCAG GTCCAGAGGA AAGCAAGTCA 
CAGGAGAGTA TGGGCAGTTC TGCAAACCCG TTTCCCAGCC TGCCCTGCCG GAACCCTTTT 
ACAGACAGGA CTGCTGCCCC TGGAAACCCA TTTAGAGTTC AGTCCCAAGA ATCAGAGGCA 
ACTTCTTGGC TCTCCAAAGA AGAGCCTGTT CCCAACAGTC CCTTCCCTCC TCTCATGCCT 
CTCAGTCATG ACACGAGCAA GGCTTCAAGT TCGCTTGGTG GCTTTGAGGA CAATTTTGAT 
CTGCAGAGCC AGTCTACAGT AAAAACAAGC AACCCCAAAG GATGGGTAAC CTTTGACGAA 
GACGACAACT TTCCTACAAC AGGAAAGTCA AAGTCAGTTT GTCCAGACTT AGTGGGTAAC 
GCGCCAGCTT CATTTGATGA TGACTGGAGT AAAGGTGCGA GCGTTTCTTT CTGTGTGTTG 
CCAGCCAGAA GGCCACCACC ACCACCACCT CCTGTCCCTC TGCTACCACC TGGCACAACC 
TCATCTGCAG GACCTAGCAC AACCCTGCCA TCTAAGGCAC CTTCCACATT GGACTTCACG 
GAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246085.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244387.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu72077
Clone ID Related Accession (Same CDS sequence)	XM_011246085.2 , XM_011246085.3
Accession Version	XM_011246085.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG GAGCAACCAA CAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244387.1
CDS	633..5000
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQEQPTG

Target ORF information:

RefSeq Version	XM_011246085.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246085.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG GAGCAACCAA CAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246087.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244389.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu72078
Clone ID Related Accession (Same CDS sequence)	XM_011246087.2 , XM_011246087.3
Accession Version	XM_011246087.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG AATCAGTGCT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244389.1
CDS	633..4997
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQNQCS

Target ORF information:

RefSeq Version	XM_011246087.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246087.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGGCTGG AAAGTTAAAA 
GATGGTGCGC GATCTGTCAC CAGAACCATC CAGAATAACT TCTTCGACAG CTCCAAGCAG 
GAAGCCATTG ATGTCCTGCT CCTGGGAAAT ACTCTCAACA GCGATTTAGC TGACAAAGCC 
CGAGCGCTTC TAACTACTGG AAGTTTGCGT GTTTCTGAAC AGACATTACA GTCAGCATCT 
TCCAAAGTCT TAAAGAACAT GTGTGAGAAC TTCTACAAAT ACTCAAAGCC CAAGAAGATC 
CGAGTGTGTG TTGGCACCTG GAATGTGAAC GGCGGGAAGC AGTTCCGCAG CATAGCGTTC 
AAGAACCAGA CGCTCACAGA CTGGCTTCTG GACGCTCCCA AGTTAGCCGG CATCCAGGAG 
TTTCAAGATA AGAGAAGTAA GCCAACTGAT ATATTTGCAA TTGGCTTTGA AGAAATGGTG 
GAGCTGAATG CTGGGAACAT TGTGAATGCA AGCACAACCA ACCAGAAGCT GTGGGCAGTG 
GAGCTGCAGA AGACCATCTC CAGGGACAAC AAGTATGTCC TGCTGGCCTC GGAGCAGCTG 
GTGGGTGTCT GCCTGTTTGT CTTTATCAGA CCACAGCACG CTCCTTTTAT CAGGGATGTT 
GCAGTTGATA CTGTGAAAAC TGGCATGGGA GGTGCAACTG GAAACAAGGG CGCCGTTGCA 
ATTCGGATGC TCTTCCACAC CACCAGCCTT TGCTTCGTCT GCAGCCACTT TGCTGCAGGG 
CAGTCCCAAG TCAAAGAGAG AAATGAAGAT TTTGTAGAAA TAGCACGAAA ACTGAGTTTC 
CCCATGGGAC GAATGCTCTT CTCCCATGAC TATGTATTTT GGTGTGGTGA TTTCAACTAT 
CGAATTGACC TTCCGAATGA AGAGGTTAAA GAGCTCATAA GACAACAAAA CTGGGATTCT 
CTGATCGCTG GGGATCAGCT CATCAACCAG AAAAATGCAG GACAGATCTT TAGAGGATTC 
TTAGAGGGAA AAGTAACGTT TGCTCCAACC TATAAATATG ACTTGTTTTC TGAAGACTAT 
GACACCAGTG AGAAGTGTCG CACCCCTGCT TGGACAGACC GTGTCCTCTG GAGAAGGAGG 
AAGTGGCCTT TTGACAGATC AGCTGAAGAT TTAGATCTCC TGAATGCTAG TTTCCAAGAT 
GAAAGTAAAA TTCTTTATAC ATGGACCCCT GGCACCTTGC TGCACTATGG AAGAGCCGAG 
CTGAAGACTT CTGACCATAG GCCCGTCGTT GCCTTGATTG ACATAGATAT ATTTGAAGTT 
GAAGCTGAAG AGAGACAAAA AATTTATAAA GAAGTTATTG CCGTCCAGGG CCCACCAGAT 
GGCACAGTGC TGGTCTCAAT CAAAAGTTCT GCACAAGAAA GTACTTTTTT TGATGATGCT 
TTGATTGATG AGCTTCTGCG GCAGTTTGCA CACTTTGGTG AAGTTATACT CATAAGATTT 
GTAGAAGATA AAATGTGGGT TACATTTTTG GAGGGAAGCT CTGCCTTGAA TGCTCTGAGC 
CTAAATGGGA AAGAGCTATT AAATCGGACT ATAACAATTA CTTTAAAAAG TCCAGACTGG 
ATCAAACATT TGGAAGAAGA GATGAGTTTA GAGAAAATCA GTGTCACGTT GCCCTCATCA 
GCAAGCTCCA CCCTGCTTGG TGAAGACGCA GAGGTCGCAG CAGACTTTGA CATGGAAGGT 
GACGTCGATG ACTACAGCGC TGAAGTAGAG GAGCTTCTTC CTCAGCATCT GCAGCCGTCC 
TCAAGTTCTG GCCTGGGCAC TTCTCCAAGT TCTTCACCCC GGACCAGTCC CTGCCAGTCC 
CCCACAGTAC CAGAGTACTC CGCCCCTTCT CTTCCCATCA GACCTAGCCG AGCACCGTCA 
AGAACTCCAG GACCTCCAAG TTCGCAGGGC TCTCCAGTTG ACACTCAGCC AGCGGCCCAG 
AAGGATTCTT CCCAGACTCT AGAGCCCAAG AGACCACCCC CTCCCCGCCC AGTCGCTCCT 
CCGGCACGTC CTGCCCCACC ACAGAGACCA CCTCCACCTT CAGGGGCTAG GAGTCCTGCG 
CCTGCTAGAA AAGAATTTGG AGGTGTTGGA GCCCCTCCCA GTCCCGGGGT TGCTAGGAGA 
GAGATAGAAG CACCCAAAAG CCCTGGAACA GCACGGAAAG ATAATATAGG GCGTAATCAG 
CCTTCCCCTC AAGCCGGACT TGCAGGCCCA GGACCCGCTG GATACGGTGC GGCTAGACCG 
ACAATTCCAG CTCGTGCTGG AGTGATCAGC GCCCCTCAGA GCCAGGCTCG GGTATGTGCT 
GGAAGGCCGA CTCCTGACAG CCAAAGCAAG CCCTCGGAGA CATTGAAAGG TCCTGCTGTC 
CTTCCAGAAC CACTGAAGCC TCAGGCTGCA TTTCCTCAGC AGCCTTCTCT GCCCACACCT 
GCTCAAAAGT TACAGGACCC GCTTGTCCCC ATAGCAGCAC CTACTATGCC TCCCTCTGGC 
CCCCAACCAA ATTTGGAAAC CCCCCCACAG CCCCCACCTC GGAGCAGGTC ATCTCAAAGC 
TTGCCTTCAG ACTCCTCACC ACAGCTGCAG AATCAGTGCT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synj1 ( XM_011246088.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244390.1 Mus musculus synaptojanin 1 (Synj1), transcript variant X17, mRNA.

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CloneID	OMu72079
Clone ID Related Accession (Same CDS sequence)	XM_011246088.3 , XM_011246088.2
Accession Version	XM_011246088.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptojanin-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246088.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGGTGTTGG AGCCCCTCCC AGTCCCGGGG TTGCTAGGAG AGAGATAGAA 
GCACCCAAAA GCCCTGGAAC AGCACGGAAA GATAATATAG GGCGTAATCA GCCTTCCCCT 
CAAGCCGGAC TTGCAGGCCC AGGACCCGCT GGATACGGTG CGGCTAGACC GACAATTCCA 
GCTCGTGCTG GAGTGATCAG CGCCCCTCAG AGCCAGGCTC GGGTATGTGC TGGAAGGCCG 
ACTCCTGACA GCCAAAGCAA GCCCTCGGAG ACATTGAAAG GTCCTGCTGT CCTTCCAGAA 
CCACTGAAGC CTCAGGCTGC ATTTCCTCAG CAGCCTTCTC TGCCCACACC TGCTCAAAAG 
TTACAGGACC CGCTTGTCCC CATAGCAGCA CCTACTATGC CTCCCTCTGG CCCCCAACCA 
AATTTGGAAA CCCCCCCACA GCCCCCACCT CGGAGCAGGT CATCTCAAAG CTTGCCTTCA 
GACTCCTCAC CACAGCTGCA GCAGGAGCAA CCAACAGGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244390.1
CDS	631..4992
Translation	MPDSSTNQHGLPQLFPEVPPCRPRGGQSYRAPREEDGPSLCRLPWPCHLPTPGATEARFTDPGRGGRRNPVTSKRPARQHGPAWAETAPPFHPKGRAFRLGSGAMRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILKPQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIRFVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTLPSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTARKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQSKPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLETPPQPPPRSRSSQSLPSDSSPQLQQEQPTG

Target ORF information:

RefSeq Version	XM_011246088.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptojanin 1 (Synj1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246088.2

ORF Insert Sequence:

ATGCCCGACT CGAGCACGAA TCAACACGGG CTCCCCCAGC TCTTTCCTGA AGTCCCTCCC 
TGTCGCCCGC GGGGAGGGCA GAGCTACAGG GCTCCACGCG AGGAGGACGG ACCCTCCCTC 
TGCCGGCTCC CCTGGCCGTG CCACCTTCCC ACTCCCGGGG CGACGGAGGC TCGCTTCACG 
GACCCAGGAC GCGGCGGCCG CAGGAACCCG GTGACCAGTA AGCGCCCTGC TCGGCAGCAC 
GGTCCCGCGT GGGCGGAAAC GGCTCCGCCC TTTCACCCTA AGGGGCGGGC CTTCCGCCTG 
GGCAGCGGCG CAATGCGGAA GAGCTGGACC TGCTGGAGCG GAAGTGACGC TTCGGGCGGC 
TGTAGCGGCG GAGGCTGCGG GAGAAGGAGG AGGAGGAGCC GGAGGAAGAG GGCTGCCTCT 
GAAGAAAGGA GAATGGCGTT CAGCAAAGGA TTTCGAATCT ACCACAAATT GGATCCCCCA 
CCGTTCAGCC TCATAGTGGA AACTAGGCAT AAGGAAGAAT GTCTCATGTT CGAGTCTGGG 
GCTGTGGCTG TGCTCTCATC TGCAGAAAAG GAGGCTATTA AAGGCACATA TGCCAAAGTA 
CTGGATGCAT ACGGACTTTT GGGAGTTTTA CGATTAAATC TTGGTGATAC CATGCTGCAC 
TATCTGGTCC TAGTCACTGG ATGCATGTCT GTGGGGAAAA TCCAAGAATC TGAAGTTTTC 
CGAGTTACTT CCACTGAGTT TATATCATTG CGAGTTGATG CTTCAGATGA GGACCGCATT 
TCGGAAGTAC GAAAAGTTTT GAACTCGGGA AACTTTTATT TTGCATGGTC TGCATCTGGA 
GTCAGCTTAG ATCTGAGTCT GAATGCACAT CGAAGCATGC AAGAGCACAC AACCGACAAT 
AGGTTTTTCT GGAATCAGTC TCTGCATTTG CATCTCAAGC ACTATGGTGT GAACTGCGAT 
GACTGGTTAT TACGGCTCAT GTGTGGGGGA GTAGAGATTA GAACCATTTA TGCTGCCCAT 
AAACAGGCAA AAGCTTGCCT CATCTCAAGA CTAAGCTGTG AACGAGCTGG GACAAGGTTC 
AATGTCCGGG GAACCAACGA TGATGGTCAT GTTGCCAACT TTGTAGAGAC AGAGCAGGTT 
ATATACTTAG ATGACTGTGT CTCTTCCTTC ATACAAATCC GAGGATCTGT TCCATTGTTC 
TGGGAGCAAC CAGGGTTGCA AGTGGGATCC CATCGTGTTC GTATGTCAAG GGGATTTGAA 
GCCAATGCGC CTGCCTTTGA CAGGCATTTC CGAACACTTA AGGACTTATA TGGTAAACAG 
ATAGTAGTGA ATCTGCTTGG GTCTAAGGAA GGCGAGCATA TGCTGAGCAA GGCTTTCCAG 
AGTCATTTAA AAGCCTCTGA ACACGCTTCT GATATCCACA TGGTGTCTTT TGACTATCAT 
CAAATGGTTA AAGGAGGAAA GGCAGAAAAA CTACACAGTA TTCTCAAACC ACAAGTCCAG 
AAGTTTCTAG ATTATGGATT TTTTTATTTC GATGGCAGTG AAGTTCAAAG GTGCCAGAGT 
GGTACAGTTC GAACAAACTG CTTGGATTGT CTGGATAGAA CAAATAGTGT GCAGGCATTC 
CTTGGCTTAG AGATGCTCGC TAAGCAGCTG GAAGCTCTGG GCTTGGCTGA GAAGCCTCAG 
CTGGTGACCC GCTTCCAAGA AGTTTTCCGG TCCATGTGGT CTGTGAATGG CGACTCAATC 
AGTAAAATAT ATGCAGGCAC CGGGGCCCTG GAAGGGAAGG CCAAGTTAAA AGATGGTGCG 
CGATCTGTCA CCAGAACCAT CCAGAATAAC TTCTTCGACA GCTCCAAGCA GGAAGCCATT 
GATGTCCTGC TCCTGGGAAA TACTCTCAAC AGCGATTTAG CTGACAAAGC CCGAGCGCTT 
CTAACTACTG GAAGTTTGCG TGTTTCTGAA CAGACATTAC AGTCAGCATC TTCCAAAGTC 
TTAAAGAACA TGTGTGAGAA CTTCTACAAA TACTCAAAGC CCAAGAAGAT CCGAGTGTGT 
GTTGGCACCT GGAATGTGAA CGGCGGGAAG CAGTTCCGCA GCATAGCGTT CAAGAACCAG 
ACGCTCACAG ACTGGCTTCT GGACGCTCCC AAGTTAGCCG GCATCCAGGA GTTTCAAGAT 
AAGAGAAGTA AGCCAACTGA TATATTTGCA ATTGGCTTTG AAGAAATGGT GGAGCTGAAT 
GCTGGGAACA TTGTGAATGC AAGCACAACC AACCAGAAGC TGTGGGCAGT GGAGCTGCAG 
AAGACCATCT CCAGGGACAA CAAGTATGTC CTGCTGGCCT CGGAGCAGCT GGTGGGTGTC 
TGCCTGTTTG TCTTTATCAG ACCACAGCAC GCTCCTTTTA TCAGGGATGT TGCAGTTGAT 
ACTGTGAAAA CTGGCATGGG AGGTGCAACT GGAAACAAGG GCGCCGTTGC AATTCGGATG 
CTCTTCCACA CCACCAGCCT TTGCTTCGTC TGCAGCCACT TTGCTGCAGG GCAGTCCCAA 
GTCAAAGAGA GAAATGAAGA TTTTGTAGAA ATAGCACGAA AACTGAGTTT CCCCATGGGA 
CGAATGCTCT TCTCCCATGA CTATGTATTT TGGTGTGGTG ATTTCAACTA TCGAATTGAC 
CTTCCGAATG AAGAGGTTAA AGAGCTCATA AGACAACAAA ACTGGGATTC TCTGATCGCT 
GGGGATCAGC TCATCAACCA GAAAAATGCA GGACAGATCT TTAGAGGATT CTTAGAGGGA 
AAAGTAACGT TTGCTCCAAC CTATAAATAT GACTTGTTTT CTGAAGACTA TGACACCAGT 
GAGAAGTGTC GCACCCCTGC TTGGACAGAC CGTGTCCTCT GGAGAAGGAG GAAGTGGCCT 
TTTGACAGAT CAGCTGAAGA TTTAGATCTC CTGAATGCTA GTTTCCAAGA TGAAAGTAAA 
ATTCTTTATA CATGGACCCC TGGCACCTTG CTGCACTATG GAAGAGCCGA GCTGAAGACT 
TCTGACCATA GGCCCGTCGT TGCCTTGATT GACATAGATA TATTTGAAGT TGAAGCTGAA 
GAGAGACAAA AAATTTATAA AGAAGTTATT GCCGTCCAGG GCCCACCAGA TGGCACAGTG 
CTGGTCTCAA TCAAAAGTTC TGCACAAGAA AGTACTTTTT TTGATGATGC TTTGATTGAT 
GAGCTTCTGC GGCAGTTTGC ACACTTTGGT GAAGTTATAC TCATAAGATT TGTAGAAGAT 
AAAATGTGGG TTACATTTTT GGAGGGAAGC TCTGCCTTGA ATGCTCTGAG CCTAAATGGG 
AAAGAGCTAT TAAATCGGAC TATAACAATT ACTTTAAAAA GTCCAGACTG GATCAAACAT 
TTGGAAGAAG AGATGAGTTT AGAGAAAATC AGTGTCACGT TGCCCTCATC AGCAAGCTCC 
ACCCTGCTTG GTGAAGACGC AGAGGTCGCA GCAGACTTTG ACATGGAAGG TGACGTCGAT 
GACTACAGCG CTGAAGTAGA GGAGCTTCTT CCTCAGCATC TGCAGCCGTC CTCAAGTTCT 
GGCCTGGGCA CTTCTCCAAG TTCTTCACCC CGGACCAGTC CCTGCCAGTC CCCCACAGTA 
CCAGAGTACT CCGCCCCTTC TCTTCCCATC AGACCTAGCC GAGCACCGTC AAGAACTCCA 
GGACCTCCAA GTTCGCAGGG CTCTCCAGTT GACACTCAGC CAGCGGCCCA GAAGGATTCT 
TCCCAGACTC TAGAGCCCAA GAGACCACCC CCTCCCCGCC CAGTCGCTCC TCCGGCACGT 
CCTGCCCCAC CACAGAGACC ACCTCCACCT TCAGGGGCTA GGAGTCCTGC GCCTGCTAGA 
AAAGAATTTG GAGGTGTTGG AGCCCCTCCC AGTCCCGGGG TTGCTAGGAG AGAGATAGAA 
GCACCCAAAA GCCCTGGAAC AGCACGGAAA GATAATATAG GGCGTAATCA GCCTTCCCCT 
CAAGCCGGAC TTGCAGGCCC AGGACCCGCT GGATACGGTG CGGCTAGACC GACAATTCCA 
GCTCGTGCTG GAGTGATCAG CGCCCCTCAG AGCCAGGCTC GGGTATGTGC TGGAAGGCCG 
ACTCCTGACA GCCAAAGCAA GCCCTCGGAG ACATTGAAAG GTCCTGCTGT CCTTCCAGAA 
CCACTGAAGC CTCAGGCTGC ATTTCCTCAG CAGCCTTCTC TGCCCACACC TGCTCAAAAG 
TTACAGGACC CGCTTGTCCC CATAGCAGCA CCTACTATGC CTCCCTCTGG CCCCCAACCA 
AATTTGGAAA CCCCCCCACA GCCCCCACCT CGGAGCAGGT CATCTCAAAG CTTGCCTTCA 
GACTCCTCAC CACAGCTGCA GCAGGAGCAA CCAACAGGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Parkinson's Disease-Associated LRRK2 Hyperactive Kinase Mutant Disrupts Synaptic Vesicle Trafficking in Ventral Midbrain Neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience37(47)11366-11376(2017 Nov)
Pan PY,Li X,Wang J,Powell J,Wang Q,Zhang Y,Chen Z,Wicinski B,Hof P,Ryan TA,Yue Z

ApoE4-associated phospholipid dysregulation contributes to development of Tau hyper-phosphorylation after traumatic brain injury.
Scientific reports7(1)11372(2017 Sep)
Cao J,Gaamouch FE,Meabon JS,Meeker KD,Zhu L,Zhong MB,Bendik J,Elder G,Jing P,Xia J,Luo W,Cook DG,Cai D

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Synj1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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