cdc42 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cdc42
Entrez Gene ID	336839
Full Name	cell division cycle 42
Synonyms	cdc42a,wu:fb07d08,wu:fj62g06,zgc:63918
General protein information	Preferred Names cell division cycle 42 Names cell division control protein 42 homolog Cdc42 protein homolog fj62g06
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200632.2	NP_956926.1	cell division control protein 42 homolog isoform 1
NM_001018120.2	NP_001018130.1	cell division control protein 42 homolog isoform 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cdc42	NP_741991.3
Danio rerio (zebrafish)	cdc42	NP_956926.1
Pan troglodytes (chimpanzee)	CDC42	XP_003949365.1
	CDC42	XP_001100552.1
Mus musculus (house mouse)	Cdc42	NP_033991.1

Gene Ontology
Bibliography

Related articles in PubMed

Bleb Expansion in Migrating Cells Depends on Supply of Membrane from Cell Surface Invaginations.
Goudarzi M, Tarbashevich K, Mildner K, Begemann I, Garcia J, Paksa A, Reichman-Fried M, Mahabaleshwar H, Blaser H, Hartwig J, Zeuschner D, Galic M, Bagnat M, Betz T, Raz E
Developmental cell43(5)577-587.e5(2017 Dec)

c-MYC drives a subset of high-risk pediatric neuroblastomas and is activated through mechanisms including enhancer hijacking and focal enhancer amplification.
Zimmerman MW, Liu Y, He S, Durbin AD, Abraham BJ, Easton J, Shao Y, Xu B, Zhu S, Zhang X, Li Z, Weichert-Leahey N, Young RA, Zhang J, Look AT
Cancer discovery(2017 Dec)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of fat1 in zebrafish causes pronephric cysts, which is partially rescued by RAC1/CDC42 activators, confirming a role of the two small GTPases in the pathogenesis.
Title: FAT1 mutations cause a glomerulotubular nephropathy.

Cdc42 deficiency causes ciliary abnormalities and cystic kidneys.
Title: Cdc42 deficiency causes ciliary abnormalities and cystic kidneys.

Cdc42 GTPase and Rac1 GTPase act downstream of p120 catenin and require GTP exchange during gastrulation of zebrafish mesoderm.
Title: Cdc42 GTPase and Rac1 GTPase act downstream of p120 catenin and require GTP exchange during gastrulation of zebrafish mesoderm.

These results suggest that Ptenb by antagonizing PI3 kinase and its downstream Akt1 and Cdc42 to regulate actin polymerization that is critical for proper cell motility and migration control during gastrulation in zebrafish.
Title: Ptenb mediates gastrulation cell movements via Cdc42/AKT1 in zebrafish.

The following cdc42 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cdc42 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00504	NM_001018120.2 Latest version!	Danio rerio cell division cycle 42 (cdc42), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa00506	NM_200632.2 Latest version!	Danio rerio cell division cycle 42 (cdc42), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cdc42 ( NM_001018120.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001018130.1 Danio rerio cell division cycle 42 (cdc42), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa00504
Clone ID Related Accession (Same CDS sequence)	NM_001018120.2
Accession Version	NM_001018120.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Danio rerio(zebrafish)
Product	cell division control protein 42 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU210919.20. On Jun 12, 2012 this sequence version replaced NM_001018120.1. Transcript Variant: This variant (2) uses an alternate 3' terminal exon compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CK355857.1, CN511112.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCAGACGA TCAAGTGCGT CGTTGTTGGT GATGGTGCAG TGGGTAAAAC CTGTCTATTA 
ATCTCCTATA CAACTAATAA ATTCCCCTCC GAATATGTAC CAACGGTCTT TGATAACTAT 
GCAGTTACGG TTATGATTGG TGGTGAGCCG TACACCCTGG GGCTGTTTGA TACTGCAGGT 
CAGGAAGATT ACGATAGATT ACGACCCCTG AGTTACCCTC AGACAGATGT CTTCTTAGTC 
TGTTTCTCAG TTGTTTCACC TTCTTCATTT GAAAACGTCA AAGAAAAGTG GGTACCCGAG 
ATCACCCACC ACTGTCCAAA GACCCCTTTC CTGCTGGTGG GGACGCAGAT TGATCTGAGA 
GATGATCCTT CTACTATCGA AAAGCTTGCC AAGAACAAAC AGAAACCCAT CACTCCAGAG 
ACGGCAGAGA AGCTGGCCCG CGATCTGAAG GCTGTCAAAT ACGTGGAATG CTCCGCTCTG 
ACGCAGCGAG GTCTGAAGAA TGTATTTGAT GAAGCTATCC TAGCTGCCCT CGAACCTCCT 
GAAACGCAGC GAAAACGGAA GTGCTGTATA TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018130.1
CDS	136..711
Translation	MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQRGLKNVFDEAILAALEPPETQRKRKCCIF

Target ORF information:

RefSeq Version	NM_001018120.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cell division cycle 42 (cdc42), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018120.2

ORF Insert Sequence:

ATGCAGACGA TCAAGTGCGT CGTTGTTGGT GATGGTGCAG TGGGTAAAAC CTGTCTATTA 
ATCTCCTATA CAACTAATAA ATTCCCCTCC GAATATGTAC CAACGGTCTT TGATAACTAT 
GCAGTTACGG TTATGATTGG TGGTGAGCCG TACACCCTGG GGCTGTTTGA TACTGCAGGT 
CAGGAAGATT ACGATAGATT ACGACCCCTG AGTTACCCTC AGACAGATGT CTTCTTAGTC 
TGTTTCTCAG TTGTTTCACC TTCTTCATTT GAAAACGTCA AAGAAAAGTG GGTACCCGAG 
ATCACCCACC ACTGTCCAAA GACCCCTTTC CTGCTGGTGG GGACGCAGAT TGATCTGAGA 
GATGATCCTT CTACTATCGA AAAGCTTGCC AAGAACAAAC AGAAACCCAT CACTCCAGAG 
ACGGCAGAGA AGCTGGCCCG CGATCTGAAG GCTGTCAAAT ACGTGGAATG CTCCGCTCTG 
ACGCAGCGAG GTCTGAAGAA TGTATTTGAT GAAGCTATCC TAGCTGCCCT CGAACCTCCT 
GAAACGCAGC GAAAACGGAA GTGCTGTATA TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cdc42 ( NM_200632.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956926.1 Danio rerio cell division cycle 42 (cdc42), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa00506
Clone ID Related Accession (Same CDS sequence)	NM_200632.2
Accession Version	NM_200632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Danio rerio(zebrafish)
Product	cell division control protein 42 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU210919.20 and EH509759.1. On Jun 12, 2012 this sequence version replaced NM_200632.1. Transcript Variant: This variant (1) represents the longer transcript. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01003527.1, GDQQ01001329.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCAGACGA TCAAGTGCGT CGTTGTTGGT GATGGTGCAG TGGGTAAAAC CTGTCTATTA 
ATCTCCTATA CAACTAATAA ATTCCCCTCC GAATATGTAC CAACGGTCTT TGATAACTAT 
GCAGTTACGG TTATGATTGG TGGTGAGCCG TACACCCTGG GGCTGTTTGA TACTGCAGGT 
CAGGAAGATT ACGATAGATT ACGACCCCTG AGTTACCCTC AGACAGATGT CTTCTTAGTC 
TGTTTCTCAG TTGTTTCACC TTCTTCATTT GAAAACGTCA AAGAAAAGTG GGTACCCGAG 
ATCACCCACC ACTGTCCAAA GACCCCTTTC CTGCTGGTGG GGACGCAGAT TGATCTGAGA 
GATGATCCTT CTACTATCGA AAAGCTTGCC AAGAACAAAC AGAAACCCAT CACTCCAGAG 
ACGGCAGAGA AGCTGGCCCG CGATCTGAAG GCTGTCAAAT ACGTGGAATG CTCCGCTCTG 
ACGCAGAAAG GCCTAAAGAA CGTATTTGAT GAGGCGATTT TGGCAGCCCT GGAGCCCCCA 
GAGCCCAAGA AGAAGCGTAA ATGTGTGCTG CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956926.1
CDS	136..711
Translation	MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKKRKCVLL

Target ORF information:

RefSeq Version	NM_200632.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cell division cycle 42 (cdc42), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200632.2

ORF Insert Sequence:

ATGCAGACGA TCAAGTGCGT CGTTGTTGGT GATGGTGCAG TGGGTAAAAC CTGTCTATTA 
ATCTCCTATA CAACTAATAA ATTCCCCTCC GAATATGTAC CAACGGTCTT TGATAACTAT 
GCAGTTACGG TTATGATTGG TGGTGAGCCG TACACCCTGG GGCTGTTTGA TACTGCAGGT 
CAGGAAGATT ACGATAGATT ACGACCCCTG AGTTACCCTC AGACAGATGT CTTCTTAGTC 
TGTTTCTCAG TTGTTTCACC TTCTTCATTT GAAAACGTCA AAGAAAAGTG GGTACCCGAG 
ATCACCCACC ACTGTCCAAA GACCCCTTTC CTGCTGGTGG GGACGCAGAT TGATCTGAGA 
GATGATCCTT CTACTATCGA AAAGCTTGCC AAGAACAAAC AGAAACCCAT CACTCCAGAG 
ACGGCAGAGA AGCTGGCCCG CGATCTGAAG GCTGTCAAAT ACGTGGAATG CTCCGCTCTG 
ACGCAGAAAG GCCTAAAGAA CGTATTTGAT GAGGCGATTT TGGCAGCCCT GGAGCCCCCA 
GAGCCCAAGA AGAAGCGTAA ATGTGTGCTG CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bleb Expansion in Migrating Cells Depends on Supply of Membrane from Cell Surface Invaginations.
Developmental cell43(5)577-587.e5(2017 Dec)
Goudarzi M,Tarbashevich K,Mildner K,Begemann I,Garcia J,Paksa A,Reichman-Fried M,Mahabaleshwar H,Blaser H,Hartwig J,Zeuschner D,Galic M,Bagnat M,Betz T,Raz E

c-MYC drives a subset of high-risk pediatric neuroblastomas and is activated through mechanisms including enhancer hijacking and focal enhancer amplification.
Cancer discovery(2017 Dec)
Zimmerman MW,Liu Y,He S,Durbin AD,Abraham BJ,Easton J,Shao Y,Xu B,Zhu S,Zhang X,Li Z,Weichert-Leahey N,Young RA,Zhang J,Look AT

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

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cdc42 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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