pls1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pls1
Entrez Gene ID	334273
Full Name	plastin 1 (I isoform)
Synonyms	wu:fi38g03,zgc:63494
General protein information	Preferred Names plastin 1 (I isoform) Names plastin-1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017357732.2	XP_017213221.1	plastin-1 isoform X1
XM_017357732.1	XP_017213221.1	plastin-1 isoform X1
NM_199881.1	NP_956175.1	plastin-1
XM_021479039.1	XP_021334714.1	plastin-1 isoform X1
XM_021479043.1	XP_021334718.1	plastin-1 isoform X2

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0719100	NP_001047943.1
Bos taurus (cattle)	PLS1	NP_001096769.1
Danio rerio (zebrafish)	pls1	NP_956175.1
Gallus gallus (chicken)	PLS1	NP_990678.1
Pan troglodytes (chimpanzee)	PLS1	XP_001162185.1
Arabidopsis thaliana (thale cress)	FIM1	NP_001031726.1
Homo sapiens (human)	PLS1	NP_002661.2
	PLS1	XP_001112046.1
Canis lupus familiaris (dog)	PLS1	XP_542817.3
Mus musculus (house mouse)	Pls1	NP_001028382.1
Arabidopsis thaliana (thale cress)	AT5G55400	NP_200351.1
Xenopus tropicalis (tropical clawed frog)	pls1	NP_001123731.1

Gene Ontology
Bibliography

Related articles in PubMed

Targeted deletion of the zebrafish actin-bundling protein L-plastin (lcp1).
Kell MJ, Riccio RE, Baumgartner EA, Compton ZJ, Pecorin PJ, Mitchell TA, Topczewski J, LeClair EE
PloS one13(1)e0190353(2018)

Sequential, Divergent, and Cooperative Requirements of
Yang YJ, Wang Y, Li Z, Zhou L, Gui JF
Genetics205(4)1551-1572(2017 04)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Live imaging of chronic inflammation caused by mutation of zebrafish Hai1.
Mathias JR, Dodd ME, Walters KB, Rhodes J, Kanki JP, Look AT, Huttenlocher A
Journal of cell science120(Pt 19)3372-83(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pls1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pls1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36608	NM_199881.1 Latest version!	Danio rerio plastin 1 (I isoform) (pls1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa150277	XM_021479043.1 Latest version!	Danio rerio plastin 1 (I isoform) (pls1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa56266	XM_021479039.1 Latest version!	Danio rerio plastin 1 (I isoform) (pls1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa56266	XM_017357732.2 Latest version!	Danio rerio plastin 1 (I isoform) (pls1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
ODa56266	XM_017357732.1	Danio rerio plastin 1 (I isoform) (pls1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pls1 ( NM_199881.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956175.1 Danio rerio plastin 1 (I isoform) (pls1), mRNA.

Price & Availability↑

CloneID	ODa36608
Clone ID Related Accession (Same CDS sequence)	NM_199881.1
Accession Version	NM_199881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	plastin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC063742.1. ##Evidence-Data-START## CDS exon combination :: BC063742.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAACA AAGTCACGCA GATCTCTCGA GAAGATCTGG AGGACCTGAG AGAAGCTTTC 
AACAAGATCG ATATCGATAA CAGTGGCTAC GTGAGTGATT TTGAGCTTCA AGAGCTCTTT 
CGTGAAGCCA GCTTTCACCT CCCGGGATAC CAGATACGGG AGATTGTGGA GAAGTTCATT 
GCCGGTGACA CCAACAAAGA TGAGAAGATC AGTTTCGAAG AGTTTGTTGC CATATCCCAA 
GAGCTGAGAA GTAAAGAAGT GAGAGAAACA TTCCGCAAGT GTATCTCCAG GCGGGATGGG 
ATTCGGTCGT TTGGTGGGAC ATCAGGTATC TCGAGTGAAG GAACGCAGCA TTCCTACTCA 
GATGAAGAAA AGGTGGCCTT TGTGAACTGG GTCAATAAAG CCTTGGCAAA CGATCCCGAC 
TGCAAACATC TGATCCCGAT GGACCCTGGG ACTGACAGTT TATTCAAATC TGTGAAAGAT 
GGAATTTTGC TGTGTAAAAT GATTAACCTG TCACAGCCAG ACACCATCGA TGAGCGGGTC 
ATCAACAAGA AATGCACTCC ATTCACAATG ACTGAGAATT TGATGTTGGC GCTCAACTCT 
GCTTCTGCGA TCGGCTGTAC GGTGGTCAAC ATCGACGCGC AGGACATGAA AGCCGGGACC 
CCTCACCTTG TGCTCGGCCT CTTGTGGCAA ATCATAAAAA TCGGCCTCTT CGCTGACATT 
GAGATCTCTC GCAACGAAGC GCTAATTGCT TTGCTGGATG AGTCAGAAGA GCTGGACCAC 
CTTATGTCAA TGTCCCCTGA GGACCTTTTG CTTCGGTGGG TCAACTATCA CCTCAAAGCT 
GCTGGATGGA AACAGATCAG AAACTTCAGT GAAGATATCA AGGACTCCAG GGCGTATTTC 
CACCTGCTTA ATCAGATCTC ACCCAAAGGA GACTTGGATG ACCAGATGAG GATTGACATT 
GACATGTTAG GACTCACCGA GCGTGATGAT GAGATGCGAG CAGAGCTGAT GTTGAGGCAG 
GCGGCTCGTC TGGACTGCAG ACAGTTTGTC ACTCCCAAGG ACGTGGTTGC AGGAAACCAT 
AAGCCCAACA TGGCGTTCGT GGCCAATCTT TTCAACATGT ACCCAGCCTT GAACAGACCC 
ACCGACAACG GCATTGATTA TGCGATCGAG GGGGAAACGA GAGAGGACAG AACATTCAGG 
AACTGGATGA ATTCTCTGGG GGTCTCTCCA TACGTTAATC ACCTGTACAG TGATCTGCAC 
GACGGCCTCA TCATTCTTCA GTTATATGAG AGAATCCAGA TACCTGTTGA CTGGCCTAGA 
GTCAACAAAC CTCCCTACCC TGTACTCGGC TCCAACATGA AGAAGCTTGA GAATTGCAAT 
TATGCGGTCG CACTGGGCAA AAAGGAAGCA AAGTTTTCCT TGGTAGGAAT CGGCGGTGAG 
AATATCAATG AGGGAAGCCC CATGCATACG CTGGCACTGG TCTGGCAACT AATGAGAAGA 
TACACACTGA AGGTTCTGTC TGATCTTGGT GATGGGGAAA AAGTCAGTGA CCAAATAATA 
ATCAACTGGG TCAACAGCAC CCTGAAACAA GGCGATAAAA ACTCCTCCAT TAGCAGCTTC 
AAGGACAAAT CGATCAGCAC AAGCCTGCCA GTGATTGATC TGATCGACAC CATTGTGCCC 
AAAGCCATTA AATACGAGAT GGTAAAGAGC GGCGACATGA CGGCAGAGGA CAAGCTAAAC 
AATGCAAAAT ATGCAATCTC TGTGGCGCGA AAAATAGGAG TCAAGGTGTA CGCCCTGCCC 
GATGACCTTG TGGAGGTCAA ACCCAAGATG GTGATGACGG TGTTCGCGTG CCTTATGGGA 
AGAGGCTTGA AGAAAGTTGA CGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956175.1
CDS	154..2040
Translation	MENKVTQISREDLEDLREAFNKIDIDNSGYVSDFELQELFREASFHLPGYQIREIVEKFIAGDTNKDEKISFEEFVAISQELRSKEVRETFRKCISRRDGIRSFGGTSGISSEGTQHSYSDEEKVAFVNWVNKALANDPDCKHLIPMDPGTDSLFKSVKDGILLCKMINLSQPDTIDERVINKKCTPFTMTENLMLALNSASAIGCTVVNIDAQDMKAGTPHLVLGLLWQIIKIGLFADIEISRNEALIALLDESEELDHLMSMSPEDLLLRWVNYHLKAAGWKQIRNFSEDIKDSRAYFHLLNQISPKGDLDDQMRIDIDMLGLTERDDEMRAELMLRQAARLDCRQFVTPKDVVAGNHKPNMAFVANLFNMYPALNRPTDNGIDYAIEGETREDRTFRNWMNSLGVSPYVNHLYSDLHDGLIILQLYERIQIPVDWPRVNKPPYPVLGSNMKKLENCNYAVALGKKEAKFSLVGIGGENINEGSPMHTLALVWQLMRRYTLKVLSDLGDGEKVSDQIIINWVNSTLKQGDKNSSISSFKDKSISTSLPVIDLIDTIVPKAIKYEMVKSGDMTAEDKLNNAKYAISVARKIGVKVYALPDDLVEVKPKMVMTVFACLMGRGLKKVDG

Target ORF information:

RefSeq Version	NM_199881.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio plastin 1 (I isoform) (pls1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199881.1

ORF Insert Sequence:

ATGGAGAACA AAGTCACGCA GATCTCTCGA GAAGATCTGG AGGACCTGAG AGAAGCTTTC 
AACAAGATCG ATATCGATAA CAGTGGCTAC GTGAGTGATT TTGAGCTTCA AGAGCTCTTT 
CGTGAAGCCA GCTTTCACCT CCCGGGATAC CAGATACGGG AGATTGTGGA GAAGTTCATT 
GCCGGTGACA CCAACAAAGA TGAGAAGATC AGTTTCGAAG AGTTTGTTGC CATATCCCAA 
GAGCTGAGAA GTAAAGAAGT GAGAGAAACA TTCCGCAAGT GTATCTCCAG GCGGGATGGG 
ATTCGGTCGT TTGGTGGGAC ATCAGGTATC TCGAGTGAAG GAACGCAGCA TTCCTACTCA 
GATGAAGAAA AGGTGGCCTT TGTGAACTGG GTCAATAAAG CCTTGGCAAA CGATCCCGAC 
TGCAAACATC TGATCCCGAT GGACCCTGGG ACTGACAGTT TATTCAAATC TGTGAAAGAT 
GGAATTTTGC TGTGTAAAAT GATTAACCTG TCACAGCCAG ACACCATCGA TGAGCGGGTC 
ATCAACAAGA AATGCACTCC ATTCACAATG ACTGAGAATT TGATGTTGGC GCTCAACTCT 
GCTTCTGCGA TCGGCTGTAC GGTGGTCAAC ATCGACGCGC AGGACATGAA AGCCGGGACC 
CCTCACCTTG TGCTCGGCCT CTTGTGGCAA ATCATAAAAA TCGGCCTCTT CGCTGACATT 
GAGATCTCTC GCAACGAAGC GCTAATTGCT TTGCTGGATG AGTCAGAAGA GCTGGACCAC 
CTTATGTCAA TGTCCCCTGA GGACCTTTTG CTTCGGTGGG TCAACTATCA CCTCAAAGCT 
GCTGGATGGA AACAGATCAG AAACTTCAGT GAAGATATCA AGGACTCCAG GGCGTATTTC 
CACCTGCTTA ATCAGATCTC ACCCAAAGGA GACTTGGATG ACCAGATGAG GATTGACATT 
GACATGTTAG GACTCACCGA GCGTGATGAT GAGATGCGAG CAGAGCTGAT GTTGAGGCAG 
GCGGCTCGTC TGGACTGCAG ACAGTTTGTC ACTCCCAAGG ACGTGGTTGC AGGAAACCAT 
AAGCCCAACA TGGCGTTCGT GGCCAATCTT TTCAACATGT ACCCAGCCTT GAACAGACCC 
ACCGACAACG GCATTGATTA TGCGATCGAG GGGGAAACGA GAGAGGACAG AACATTCAGG 
AACTGGATGA ATTCTCTGGG GGTCTCTCCA TACGTTAATC ACCTGTACAG TGATCTGCAC 
GACGGCCTCA TCATTCTTCA GTTATATGAG AGAATCCAGA TACCTGTTGA CTGGCCTAGA 
GTCAACAAAC CTCCCTACCC TGTACTCGGC TCCAACATGA AGAAGCTTGA GAATTGCAAT 
TATGCGGTCG CACTGGGCAA AAAGGAAGCA AAGTTTTCCT TGGTAGGAAT CGGCGGTGAG 
AATATCAATG AGGGAAGCCC CATGCATACG CTGGCACTGG TCTGGCAACT AATGAGAAGA 
TACACACTGA AGGTTCTGTC TGATCTTGGT GATGGGGAAA AAGTCAGTGA CCAAATAATA 
ATCAACTGGG TCAACAGCAC CCTGAAACAA GGCGATAAAA ACTCCTCCAT TAGCAGCTTC 
AAGGACAAAT CGATCAGCAC AAGCCTGCCA GTGATTGATC TGATCGACAC CATTGTGCCC 
AAAGCCATTA AATACGAGAT GGTAAAGAGC GGCGACATGA CGGCAGAGGA CAAGCTAAAC 
AATGCAAAAT ATGCAATCTC TGTGGCGCGA AAAATAGGAG TCAAGGTGTA CGCCCTGCCC 
GATGACCTTG TGGAGGTCAA ACCCAAGATG GTGATGACGG TGTTCGCGTG CCTTATGGGA 
AGAGGCTTGA AGAAAGTTGA CGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pls1 ( XM_021479043.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334718.1 Danio rerio plastin 1 (I isoform) (pls1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa150277
Clone ID Related Accession (Same CDS sequence)	XM_021479043.1
Accession Version	XM_021479043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plastin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACA AAGTCACGCA GATCTCTCGA GAAGATCTGG AGGACCTGAG AGAAGCTTTC 
AACAAGATTG ATATCGATAA CAGTGGCTAC GTGAGTGATT TTGAGCTTCA AGAGCTCTTT 
CGTGAAGCCA GCTTTCACCT CCCGGGATAC CAGATACGGG AGATTGTAGA GAAGTTCATT 
GCCGGTGACA CCAACAAAGA TGAGAAGATC AGTTTCGAAG AGTTTGTTGC CATATCCCAA 
GAGCTGAGAA GTAAAGAAGT GAGAGAAACA TTCCGCAAGT GTATCTCCAG GCGGGATGGG 
ATTCGGTCGT TTGGTGGGAC ATCAGGTATC TCGAGTGAAG GAACGCAGCA TTCCTACTCA 
GATGAAGAAA AGGTGGCCTT TGTGAACTGG GTCAATAAAG CTTTGGCAAA CGATCCCGAC 
TGCAAACATC TGATCCCGAT GGACCCTGGG ACTGACAGTT TATTCAAATC TGTGAAAGAT 
GGAATTTTGC TGTGTAAAAT GATTAACCTG TCACAGCCAG ACACCATCGA TGAGCGGGTC 
ATCAACAAGA AATGCACTCC ATTCACAATG ACTGAGAATT TGATGTTGGC GCTCAACTCT 
GCTTCTGCGA TCGGCTGTAC GGTGGTCAAC ATCGACGCGC AGGACATGAA AGCCGGGACC 
CCTCACCTTG TGCTCGGCCT CTTGTGGCAA ATCATAAAAA TCGGCCTCTT CGCTGACATT 
GAGATCTCTC GCAACGAAGC GCTAATTGCT TTGCTGGATG AGTCAGAAGA GCTGGACCAC 
CTTATGTCAA TGTCCCCTGA GGACCTTTTG CTTCGGTGGG TCAACTATCA CCTCAAAGCT 
GCTGGATGGA AACAGATCAG AAACTTCAGT GAAGATATCA AGGACTCCAG GGCGTATTTC 
CACCTGCTTA ATCAGATCTC ACCCAAAGGA GACTTGGATG ACCAGATGAG GATTGACATT 
GACATGTTAG GACTCACCGA GCGTGATGAT GAGATGCGAG CAGAGCTGAT GTTGAGGCAG 
GCGGCTCGTC TGGACTGCAG ACAGTTTGTC ACTCCCAAGG ACGTGGTTGC AGGAAACCAT 
AAGCTCAACA TGGCGTTCGT GGCCAATCTT TTCAACATGT ACCCAGCCTT GAACAGACCC 
ACCGACAACG GCATTGATTA TGCGATCGAG GGGGAAACGA GAGAGGACAG AACATTCAGG 
AACTGGATGA ATTCTCTGGG GGTCTCTCCA TACGTTAATC ACCTGTACAG TGATCTGCAC 
GACGGCCTCA TCATTCTTCA GTTATATGAG AGAATCCAGA TACCGGTTGA CTGGCCTAGA 
GTCAACAAAC CTCCCTACCC TGTACTCGGC TCCAACATGA AGAAGCTTGA GAATTGCAAT 
TATGCGGTCG CACTGGGCAA AAAGGAAGCA AAGTTTTCCT TGGTAGGAAT CGGCGGTGAG 
AATATCAATG AGGGAAGCCC CATGCACACG CTGGCACTGG TCTGGCAACT AATGAGAAGA 
TACACACTGA AGGTTCTGTC TGATCTTGGT GATGGGGAAA AAGTCAGTGA CCAAATAATA 
ATCAACTGGG TGAACAGCAC TCTGAAACAA GGCGATAAAA ACTCCTCCAT TAGCAGCTTC 
AAGGACAAAT CGATCAGCAC AAGCCTGCCA GTGATTGATC TGATCGACAC CATTGTGCCC 
AAAGCCATTA AATACGAGAT GGTGAAGAGC GGCGACATGA CGGCAGAGGA CAAGCTAAAC 
AATGCAAAAT ATGCAATCTC TGTGGCGCGA AAAATAGGAG TCAAGGTGTA CGCCCTGCCC 
GATGACCTTG TGGAGGTCAA ACCCAAGATG GTGATGACGG TGTTCGCGTG CCTTATGGGA 
AGAGGCTTGA AGAAAGTTGA CGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334718.1
CDS	147..2033
Translation	MENKVTQISREDLEDLREAFNKIDIDNSGYVSDFELQELFREASFHLPGYQIREIVEKFIAGDTNKDEKISFEEFVAISQELRSKEVRETFRKCISRRDGIRSFGGTSGISSEGTQHSYSDEEKVAFVNWVNKALANDPDCKHLIPMDPGTDSLFKSVKDGILLCKMINLSQPDTIDERVINKKCTPFTMTENLMLALNSASAIGCTVVNIDAQDMKAGTPHLVLGLLWQIIKIGLFADIEISRNEALIALLDESEELDHLMSMSPEDLLLRWVNYHLKAAGWKQIRNFSEDIKDSRAYFHLLNQISPKGDLDDQMRIDIDMLGLTERDDEMRAELMLRQAARLDCRQFVTPKDVVAGNHKLNMAFVANLFNMYPALNRPTDNGIDYAIEGETREDRTFRNWMNSLGVSPYVNHLYSDLHDGLIILQLYERIQIPVDWPRVNKPPYPVLGSNMKKLENCNYAVALGKKEAKFSLVGIGGENINEGSPMHTLALVWQLMRRYTLKVLSDLGDGEKVSDQIIINWVNSTLKQGDKNSSISSFKDKSISTSLPVIDLIDTIVPKAIKYEMVKSGDMTAEDKLNNAKYAISVARKIGVKVYALPDDLVEVKPKMVMTVFACLMGRGLKKVDG

Target ORF information:

RefSeq Version	XM_021479043.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio plastin 1 (I isoform) (pls1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479043.1

ORF Insert Sequence:

ATGGAGAACA AAGTCACGCA GATCTCTCGA GAAGATCTGG AGGACCTGAG AGAAGCTTTC 
AACAAGATTG ATATCGATAA CAGTGGCTAC GTGAGTGATT TTGAGCTTCA AGAGCTCTTT 
CGTGAAGCCA GCTTTCACCT CCCGGGATAC CAGATACGGG AGATTGTAGA GAAGTTCATT 
GCCGGTGACA CCAACAAAGA TGAGAAGATC AGTTTCGAAG AGTTTGTTGC CATATCCCAA 
GAGCTGAGAA GTAAAGAAGT GAGAGAAACA TTCCGCAAGT GTATCTCCAG GCGGGATGGG 
ATTCGGTCGT TTGGTGGGAC ATCAGGTATC TCGAGTGAAG GAACGCAGCA TTCCTACTCA 
GATGAAGAAA AGGTGGCCTT TGTGAACTGG GTCAATAAAG CTTTGGCAAA CGATCCCGAC 
TGCAAACATC TGATCCCGAT GGACCCTGGG ACTGACAGTT TATTCAAATC TGTGAAAGAT 
GGAATTTTGC TGTGTAAAAT GATTAACCTG TCACAGCCAG ACACCATCGA TGAGCGGGTC 
ATCAACAAGA AATGCACTCC ATTCACAATG ACTGAGAATT TGATGTTGGC GCTCAACTCT 
GCTTCTGCGA TCGGCTGTAC GGTGGTCAAC ATCGACGCGC AGGACATGAA AGCCGGGACC 
CCTCACCTTG TGCTCGGCCT CTTGTGGCAA ATCATAAAAA TCGGCCTCTT CGCTGACATT 
GAGATCTCTC GCAACGAAGC GCTAATTGCT TTGCTGGATG AGTCAGAAGA GCTGGACCAC 
CTTATGTCAA TGTCCCCTGA GGACCTTTTG CTTCGGTGGG TCAACTATCA CCTCAAAGCT 
GCTGGATGGA AACAGATCAG AAACTTCAGT GAAGATATCA AGGACTCCAG GGCGTATTTC 
CACCTGCTTA ATCAGATCTC ACCCAAAGGA GACTTGGATG ACCAGATGAG GATTGACATT 
GACATGTTAG GACTCACCGA GCGTGATGAT GAGATGCGAG CAGAGCTGAT GTTGAGGCAG 
GCGGCTCGTC TGGACTGCAG ACAGTTTGTC ACTCCCAAGG ACGTGGTTGC AGGAAACCAT 
AAGCTCAACA TGGCGTTCGT GGCCAATCTT TTCAACATGT ACCCAGCCTT GAACAGACCC 
ACCGACAACG GCATTGATTA TGCGATCGAG GGGGAAACGA GAGAGGACAG AACATTCAGG 
AACTGGATGA ATTCTCTGGG GGTCTCTCCA TACGTTAATC ACCTGTACAG TGATCTGCAC 
GACGGCCTCA TCATTCTTCA GTTATATGAG AGAATCCAGA TACCGGTTGA CTGGCCTAGA 
GTCAACAAAC CTCCCTACCC TGTACTCGGC TCCAACATGA AGAAGCTTGA GAATTGCAAT 
TATGCGGTCG CACTGGGCAA AAAGGAAGCA AAGTTTTCCT TGGTAGGAAT CGGCGGTGAG 
AATATCAATG AGGGAAGCCC CATGCACACG CTGGCACTGG TCTGGCAACT AATGAGAAGA 
TACACACTGA AGGTTCTGTC TGATCTTGGT GATGGGGAAA AAGTCAGTGA CCAAATAATA 
ATCAACTGGG TGAACAGCAC TCTGAAACAA GGCGATAAAA ACTCCTCCAT TAGCAGCTTC 
AAGGACAAAT CGATCAGCAC AAGCCTGCCA GTGATTGATC TGATCGACAC CATTGTGCCC 
AAAGCCATTA AATACGAGAT GGTGAAGAGC GGCGACATGA CGGCAGAGGA CAAGCTAAAC 
AATGCAAAAT ATGCAATCTC TGTGGCGCGA AAAATAGGAG TCAAGGTGTA CGCCCTGCCC 
GATGACCTTG TGGAGGTCAA ACCCAAGATG GTGATGACGG TGTTCGCGTG CCTTATGGGA 
AGAGGCTTGA AGAAAGTTGA CGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pls1 ( XM_017357732.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213221.1 Danio rerio plastin 1 (I isoform) (pls1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa56266
Clone ID Related Accession (Same CDS sequence)	XM_017357732.2 , XM_017357732.1 , XM_021479039.1
Accession Version	XM_017357732.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plastin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTCA TCACCAGAGG ACTCCACAGG ATATTCTTGT GGACCAAAAG ACAAAATAGT 
ACAATTACAG GACCCTTTTT GCATGGTGTG AAGTGCTTCG AGGAGACCAG CATGGAGAAC 
AAAGTCACGC AGATCTCTCG AGAAGATCTG GAGGACCTGA GAGAAGCTTT CAACAAGATT 
GATATCGATA ACAGTGGCTA CGTGAGTGAT TTTGAGCTTC AAGAGCTCTT TCGTGAAGCC 
AGCTTTCACC TCCCGGGATA CCAGATACGG GAGATTGTAG AGAAGTTCAT TGCCGGTGAC 
ACCAACAAAG ATGAGAAGAT CAGTTTCGAA GAGTTTGTTG CCATATCCCA AGAGCTGAGA 
AGTAAAGAAG TGAGAGAAAC ATTCCGCAAG TGTATCTCCA GGCGGGATGG GATTCGGTCG 
TTTGGTGGGA CATCAGGTAT CTCGAGTGAA GGAACGCAGC ATTCCTACTC AGATGAAGAA 
AAGGTGGCCT TTGTGAACTG GGTCAATAAA GCTTTGGCAA ACGATCCCGA CTGCAAACAT 
CTGATCCCGA TGGACCCTGG GACTGACAGT TTATTCAAAT CTGTGAAAGA TGGAATTTTG 
CTGTGTAAAA TGATTAACCT GTCACAGCCA GACACCATCG ATGAGCGGGT CATCAACAAG 
AAATGCACTC CATTCACAAT GACTGAGAAT TTGATGTTGG CGCTCAACTC TGCTTCTGCG 
ATCGGCTGTA CGGTGGTCAA CATCGACGCG CAGGACATGA AAGCCGGGAC CCCTCACCTT 
GTGCTCGGCC TCTTGTGGCA AATCATAAAA ATCGGCCTCT TCGCTGACAT TGAGATCTCT 
CGCAACGAAG CGCTAATTGC TTTGCTGGAT GAGTCAGAAG AGCTGGACCA CCTTATGTCA 
ATGTCCCCTG AGGACCTTTT GCTTCGGTGG GTCAACTATC ACCTCAAAGC TGCTGGATGG 
AAACAGATCA GAAACTTCAG TGAAGATATC AAGGACTCCA GGGCGTATTT CCACCTGCTT 
AATCAGATCT CACCCAAAGG AGACTTGGAT GACCAGATGA GGATTGACAT TGACATGTTA 
GGACTCACCG AGCGTGATGA TGAGATGCGA GCAGAGCTGA TGTTGAGGCA GGCGGCTCGT 
CTGGACTGCA GACAGTTTGT CACTCCCAAG GACGTGGTTG CAGGAAACCA TAAGCTCAAC 
ATGGCGTTCG TGGCCAATCT TTTCAACATG TACCCAGCCT TGAACAGACC CACCGACAAC 
GGCATTGATT ATGCGATCGA GGGGGAAACG AGAGAGGACA GAACATTCAG GAACTGGATG 
AATTCTCTGG GGGTCTCTCC ATACGTTAAT CACCTGTACA GTGATCTGCA CGACGGCCTC 
ATCATTCTTC AGTTATATGA GAGAATCCAG ATACCGGTTG ACTGGCCTAG AGTCAACAAA 
CCTCCCTACC CTGTACTCGG CTCCAACATG AAGAAGCTTG AGAATTGCAA TTATGCGGTC 
GCACTGGGCA AAAAGGAAGC AAAGTTTTCC TTGGTAGGAA TCGGCGGTGA GAATATCAAT 
GAGGGAAGCC CCATGCACAC GCTGGCACTG GTCTGGCAAC TAATGAGAAG ATACACACTG 
AAGGTTCTGT CTGATCTTGG TGATGGGGAA AAAGTCAGTG ACCAAATAAT AATCAACTGG 
GTGAACAGCA CTCTGAAACA AGGCGATAAA AACTCCTCCA TTAGCAGCTT CAAGGACAAA 
TCGATCAGCA CAAGCCTGCC AGTGATTGAT CTGATCGACA CCATTGTGCC CAAAGCCATT 
AAATACGAGA TGGTGAAGAG CGGCGACATG ACGGCAGAGG ACAAGCTAAA CAATGCAAAA 
TATGCAATCT CTGTGGCGCG AAAAATAGGA GTCAAGGTGT ACGCCCTGCC CGATGACCTT 
GTGGAGGTCA AACCCAAGAT GGTGATGACG GTGTTCGCGT GCCTTATGGG AAGAGGCTTG 
AAGAAAGTTG ACGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213221.1
CDS	632..2629
Translation	MALITRGLHRIFLWTKRQNSTITGPFLHGVKCFEETSMENKVTQISREDLEDLREAFNKIDIDNSGYVSDFELQELFREASFHLPGYQIREIVEKFIAGDTNKDEKISFEEFVAISQELRSKEVRETFRKCISRRDGIRSFGGTSGISSEGTQHSYSDEEKVAFVNWVNKALANDPDCKHLIPMDPGTDSLFKSVKDGILLCKMINLSQPDTIDERVINKKCTPFTMTENLMLALNSASAIGCTVVNIDAQDMKAGTPHLVLGLLWQIIKIGLFADIEISRNEALIALLDESEELDHLMSMSPEDLLLRWVNYHLKAAGWKQIRNFSEDIKDSRAYFHLLNQISPKGDLDDQMRIDIDMLGLTERDDEMRAELMLRQAARLDCRQFVTPKDVVAGNHKLNMAFVANLFNMYPALNRPTDNGIDYAIEGETREDRTFRNWMNSLGVSPYVNHLYSDLHDGLIILQLYERIQIPVDWPRVNKPPYPVLGSNMKKLENCNYAVALGKKEAKFSLVGIGGENINEGSPMHTLALVWQLMRRYTLKVLSDLGDGEKVSDQIIINWVNSTLKQGDKNSSISSFKDKSISTSLPVIDLIDTIVPKAIKYEMVKSGDMTAEDKLNNAKYAISVARKIGVKVYALPDDLVEVKPKMVMTVFACLMGRGLKKVDG

Target ORF information:

RefSeq Version	XM_017357732.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio plastin 1 (I isoform) (pls1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357732.2

ORF Insert Sequence:

ATGGCGCTCA TCACCAGAGG ACTCCACAGG ATATTCTTGT GGACCAAAAG ACAAAATAGT 
ACAATTACAG GACCCTTTTT GCATGGTGTG AAGTGCTTCG AGGAGACCAG CATGGAGAAC 
AAAGTCACGC AGATCTCTCG AGAAGATCTG GAGGACCTGA GAGAAGCTTT CAACAAGATT 
GATATCGATA ACAGTGGCTA CGTGAGTGAT TTTGAGCTTC AAGAGCTCTT TCGTGAAGCC 
AGCTTTCACC TCCCGGGATA CCAGATACGG GAGATTGTAG AGAAGTTCAT TGCCGGTGAC 
ACCAACAAAG ATGAGAAGAT CAGTTTCGAA GAGTTTGTTG CCATATCCCA AGAGCTGAGA 
AGTAAAGAAG TGAGAGAAAC ATTCCGCAAG TGTATCTCCA GGCGGGATGG GATTCGGTCG 
TTTGGTGGGA CATCAGGTAT CTCGAGTGAA GGAACGCAGC ATTCCTACTC AGATGAAGAA 
AAGGTGGCCT TTGTGAACTG GGTCAATAAA GCTTTGGCAA ACGATCCCGA CTGCAAACAT 
CTGATCCCGA TGGACCCTGG GACTGACAGT TTATTCAAAT CTGTGAAAGA TGGAATTTTG 
CTGTGTAAAA TGATTAACCT GTCACAGCCA GACACCATCG ATGAGCGGGT CATCAACAAG 
AAATGCACTC CATTCACAAT GACTGAGAAT TTGATGTTGG CGCTCAACTC TGCTTCTGCG 
ATCGGCTGTA CGGTGGTCAA CATCGACGCG CAGGACATGA AAGCCGGGAC CCCTCACCTT 
GTGCTCGGCC TCTTGTGGCA AATCATAAAA ATCGGCCTCT TCGCTGACAT TGAGATCTCT 
CGCAACGAAG CGCTAATTGC TTTGCTGGAT GAGTCAGAAG AGCTGGACCA CCTTATGTCA 
ATGTCCCCTG AGGACCTTTT GCTTCGGTGG GTCAACTATC ACCTCAAAGC TGCTGGATGG 
AAACAGATCA GAAACTTCAG TGAAGATATC AAGGACTCCA GGGCGTATTT CCACCTGCTT 
AATCAGATCT CACCCAAAGG AGACTTGGAT GACCAGATGA GGATTGACAT TGACATGTTA 
GGACTCACCG AGCGTGATGA TGAGATGCGA GCAGAGCTGA TGTTGAGGCA GGCGGCTCGT 
CTGGACTGCA GACAGTTTGT CACTCCCAAG GACGTGGTTG CAGGAAACCA TAAGCTCAAC 
ATGGCGTTCG TGGCCAATCT TTTCAACATG TACCCAGCCT TGAACAGACC CACCGACAAC 
GGCATTGATT ATGCGATCGA GGGGGAAACG AGAGAGGACA GAACATTCAG GAACTGGATG 
AATTCTCTGG GGGTCTCTCC ATACGTTAAT CACCTGTACA GTGATCTGCA CGACGGCCTC 
ATCATTCTTC AGTTATATGA GAGAATCCAG ATACCGGTTG ACTGGCCTAG AGTCAACAAA 
CCTCCCTACC CTGTACTCGG CTCCAACATG AAGAAGCTTG AGAATTGCAA TTATGCGGTC 
GCACTGGGCA AAAAGGAAGC AAAGTTTTCC TTGGTAGGAA TCGGCGGTGA GAATATCAAT 
GAGGGAAGCC CCATGCACAC GCTGGCACTG GTCTGGCAAC TAATGAGAAG ATACACACTG 
AAGGTTCTGT CTGATCTTGG TGATGGGGAA AAAGTCAGTG ACCAAATAAT AATCAACTGG 
GTGAACAGCA CTCTGAAACA AGGCGATAAA AACTCCTCCA TTAGCAGCTT CAAGGACAAA 
TCGATCAGCA CAAGCCTGCC AGTGATTGAT CTGATCGACA CCATTGTGCC CAAAGCCATT 
AAATACGAGA TGGTGAAGAG CGGCGACATG ACGGCAGAGG ACAAGCTAAA CAATGCAAAA 
TATGCAATCT CTGTGGCGCG AAAAATAGGA GTCAAGGTGT ACGCCCTGCC CGATGACCTT 
GTGGAGGTCA AACCCAAGAT GGTGATGACG GTGTTCGCGT GCCTTATGGG AAGAGGCTTG 
AAGAAAGTTG ACGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pls1 ( XM_017357732.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213221.1 Danio rerio plastin 1 (I isoform) (pls1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56266
Clone ID Related Accession (Same CDS sequence)	XM_017357732.2 , XM_017357732.1 , XM_021479039.1
Accession Version	XM_017357732.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	plastin-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTCA TCACCAGAGG ACTCCACAGG ATATTCTTGT GGACCAAAAG ACAAAATAGT 
ACAATTACAG GACCCTTTTT GCATGGTGTG AAGTGCTTCG AGGAGACCAG CATGGAGAAC 
AAAGTCACGC AGATCTCTCG AGAAGATCTG GAGGACCTGA GAGAAGCTTT CAACAAGATT 
GATATCGATA ACAGTGGCTA CGTGAGTGAT TTTGAGCTTC AAGAGCTCTT TCGTGAAGCC 
AGCTTTCACC TCCCGGGATA CCAGATACGG GAGATTGTAG AGAAGTTCAT TGCCGGTGAC 
ACCAACAAAG ATGAGAAGAT CAGTTTCGAA GAGTTTGTTG CCATATCCCA AGAGCTGAGA 
AGTAAAGAAG TGAGAGAAAC ATTCCGCAAG TGTATCTCCA GGCGGGATGG GATTCGGTCG 
TTTGGTGGGA CATCAGGTAT CTCGAGTGAA GGAACGCAGC ATTCCTACTC AGATGAAGAA 
AAGGTGGCCT TTGTGAACTG GGTCAATAAA GCTTTGGCAA ACGATCCCGA CTGCAAACAT 
CTGATCCCGA TGGACCCTGG GACTGACAGT TTATTCAAAT CTGTGAAAGA TGGAATTTTG 
CTGTGTAAAA TGATTAACCT GTCACAGCCA GACACCATCG ATGAGCGGGT CATCAACAAG 
AAATGCACTC CATTCACAAT GACTGAGAAT TTGATGTTGG CGCTCAACTC TGCTTCTGCG 
ATCGGCTGTA CGGTGGTCAA CATCGACGCG CAGGACATGA AAGCCGGGAC CCCTCACCTT 
GTGCTCGGCC TCTTGTGGCA AATCATAAAA ATCGGCCTCT TCGCTGACAT TGAGATCTCT 
CGCAACGAAG CGCTAATTGC TTTGCTGGAT GAGTCAGAAG AGCTGGACCA CCTTATGTCA 
ATGTCCCCTG AGGACCTTTT GCTTCGGTGG GTCAACTATC ACCTCAAAGC TGCTGGATGG 
AAACAGATCA GAAACTTCAG TGAAGATATC AAGGACTCCA GGGCGTATTT CCACCTGCTT 
AATCAGATCT CACCCAAAGG AGACTTGGAT GACCAGATGA GGATTGACAT TGACATGTTA 
GGACTCACCG AGCGTGATGA TGAGATGCGA GCAGAGCTGA TGTTGAGGCA GGCGGCTCGT 
CTGGACTGCA GACAGTTTGT CACTCCCAAG GACGTGGTTG CAGGAAACCA TAAGCTCAAC 
ATGGCGTTCG TGGCCAATCT TTTCAACATG TACCCAGCCT TGAACAGACC CACCGACAAC 
GGCATTGATT ATGCGATCGA GGGGGAAACG AGAGAGGACA GAACATTCAG GAACTGGATG 
AATTCTCTGG GGGTCTCTCC ATACGTTAAT CACCTGTACA GTGATCTGCA CGACGGCCTC 
ATCATTCTTC AGTTATATGA GAGAATCCAG ATACCGGTTG ACTGGCCTAG AGTCAACAAA 
CCTCCCTACC CTGTACTCGG CTCCAACATG AAGAAGCTTG AGAATTGCAA TTATGCGGTC 
GCACTGGGCA AAAAGGAAGC AAAGTTTTCC TTGGTAGGAA TCGGCGGTGA GAATATCAAT 
GAGGGAAGCC CCATGCACAC GCTGGCACTG GTCTGGCAAC TAATGAGAAG ATACACACTG 
AAGGTTCTGT CTGATCTTGG TGATGGGGAA AAAGTCAGTG ACCAAATAAT AATCAACTGG 
GTGAACAGCA CTCTGAAACA AGGCGATAAA AACTCCTCCA TTAGCAGCTT CAAGGACAAA 
TCGATCAGCA CAAGCCTGCC AGTGATTGAT CTGATCGACA CCATTGTGCC CAAAGCCATT 
AAATACGAGA TGGTGAAGAG CGGCGACATG ACGGCAGAGG ACAAGCTAAA CAATGCAAAA 
TATGCAATCT CTGTGGCGCG AAAAATAGGA GTCAAGGTGT ACGCCCTGCC CGATGACCTT 
GTGGAGGTCA AACCCAAGAT GGTGATGACG GTGTTCGCGT GCCTTATGGG AAGAGGCTTG 
AAGAAAGTTG ACGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213221.1
CDS	686..2683
Translation	MALITRGLHRIFLWTKRQNSTITGPFLHGVKCFEETSMENKVTQISREDLEDLREAFNKIDIDNSGYVSDFELQELFREASFHLPGYQIREIVEKFIAGDTNKDEKISFEEFVAISQELRSKEVRETFRKCISRRDGIRSFGGTSGISSEGTQHSYSDEEKVAFVNWVNKALANDPDCKHLIPMDPGTDSLFKSVKDGILLCKMINLSQPDTIDERVINKKCTPFTMTENLMLALNSASAIGCTVVNIDAQDMKAGTPHLVLGLLWQIIKIGLFADIEISRNEALIALLDESEELDHLMSMSPEDLLLRWVNYHLKAAGWKQIRNFSEDIKDSRAYFHLLNQISPKGDLDDQMRIDIDMLGLTERDDEMRAELMLRQAARLDCRQFVTPKDVVAGNHKLNMAFVANLFNMYPALNRPTDNGIDYAIEGETREDRTFRNWMNSLGVSPYVNHLYSDLHDGLIILQLYERIQIPVDWPRVNKPPYPVLGSNMKKLENCNYAVALGKKEAKFSLVGIGGENINEGSPMHTLALVWQLMRRYTLKVLSDLGDGEKVSDQIIINWVNSTLKQGDKNSSISSFKDKSISTSLPVIDLIDTIVPKAIKYEMVKSGDMTAEDKLNNAKYAISVARKIGVKVYALPDDLVEVKPKMVMTVFACLMGRGLKKVDG

Target ORF information:

RefSeq Version	XM_017357732.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio plastin 1 (I isoform) (pls1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357732.1

ORF Insert Sequence:

ATGGCGCTCA TCACCAGAGG ACTCCACAGG ATATTCTTGT GGACCAAAAG ACAAAATAGT 
ACAATTACAG GACCCTTTTT GCATGGTGTG AAGTGCTTCG AGGAGACCAG CATGGAGAAC 
AAAGTCACGC AGATCTCTCG AGAAGATCTG GAGGACCTGA GAGAAGCTTT CAACAAGATT 
GATATCGATA ACAGTGGCTA CGTGAGTGAT TTTGAGCTTC AAGAGCTCTT TCGTGAAGCC 
AGCTTTCACC TCCCGGGATA CCAGATACGG GAGATTGTAG AGAAGTTCAT TGCCGGTGAC 
ACCAACAAAG ATGAGAAGAT CAGTTTCGAA GAGTTTGTTG CCATATCCCA AGAGCTGAGA 
AGTAAAGAAG TGAGAGAAAC ATTCCGCAAG TGTATCTCCA GGCGGGATGG GATTCGGTCG 
TTTGGTGGGA CATCAGGTAT CTCGAGTGAA GGAACGCAGC ATTCCTACTC AGATGAAGAA 
AAGGTGGCCT TTGTGAACTG GGTCAATAAA GCTTTGGCAA ACGATCCCGA CTGCAAACAT 
CTGATCCCGA TGGACCCTGG GACTGACAGT TTATTCAAAT CTGTGAAAGA TGGAATTTTG 
CTGTGTAAAA TGATTAACCT GTCACAGCCA GACACCATCG ATGAGCGGGT CATCAACAAG 
AAATGCACTC CATTCACAAT GACTGAGAAT TTGATGTTGG CGCTCAACTC TGCTTCTGCG 
ATCGGCTGTA CGGTGGTCAA CATCGACGCG CAGGACATGA AAGCCGGGAC CCCTCACCTT 
GTGCTCGGCC TCTTGTGGCA AATCATAAAA ATCGGCCTCT TCGCTGACAT TGAGATCTCT 
CGCAACGAAG CGCTAATTGC TTTGCTGGAT GAGTCAGAAG AGCTGGACCA CCTTATGTCA 
ATGTCCCCTG AGGACCTTTT GCTTCGGTGG GTCAACTATC ACCTCAAAGC TGCTGGATGG 
AAACAGATCA GAAACTTCAG TGAAGATATC AAGGACTCCA GGGCGTATTT CCACCTGCTT 
AATCAGATCT CACCCAAAGG AGACTTGGAT GACCAGATGA GGATTGACAT TGACATGTTA 
GGACTCACCG AGCGTGATGA TGAGATGCGA GCAGAGCTGA TGTTGAGGCA GGCGGCTCGT 
CTGGACTGCA GACAGTTTGT CACTCCCAAG GACGTGGTTG CAGGAAACCA TAAGCTCAAC 
ATGGCGTTCG TGGCCAATCT TTTCAACATG TACCCAGCCT TGAACAGACC CACCGACAAC 
GGCATTGATT ATGCGATCGA GGGGGAAACG AGAGAGGACA GAACATTCAG GAACTGGATG 
AATTCTCTGG GGGTCTCTCC ATACGTTAAT CACCTGTACA GTGATCTGCA CGACGGCCTC 
ATCATTCTTC AGTTATATGA GAGAATCCAG ATACCGGTTG ACTGGCCTAG AGTCAACAAA 
CCTCCCTACC CTGTACTCGG CTCCAACATG AAGAAGCTTG AGAATTGCAA TTATGCGGTC 
GCACTGGGCA AAAAGGAAGC AAAGTTTTCC TTGGTAGGAA TCGGCGGTGA GAATATCAAT 
GAGGGAAGCC CCATGCACAC GCTGGCACTG GTCTGGCAAC TAATGAGAAG ATACACACTG 
AAGGTTCTGT CTGATCTTGG TGATGGGGAA AAAGTCAGTG ACCAAATAAT AATCAACTGG 
GTGAACAGCA CTCTGAAACA AGGCGATAAA AACTCCTCCA TTAGCAGCTT CAAGGACAAA 
TCGATCAGCA CAAGCCTGCC AGTGATTGAT CTGATCGACA CCATTGTGCC CAAAGCCATT 
AAATACGAGA TGGTGAAGAG CGGCGACATG ACGGCAGAGG ACAAGCTAAA CAATGCAAAA 
TATGCAATCT CTGTGGCGCG AAAAATAGGA GTCAAGGTGT ACGCCCTGCC CGATGACCTT 
GTGGAGGTCA AACCCAAGAT GGTGATGACG GTGTTCGCGT GCCTTATGGG AAGAGGCTTG 
AAGAAAGTTG ACGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pls1 ( XM_021479039.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334714.1 Danio rerio plastin 1 (I isoform) (pls1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56266
Clone ID Related Accession (Same CDS sequence)	XM_017357732.2 , XM_017357732.1 , XM_021479039.1
Accession Version	XM_021479039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plastin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTCA TCACCAGAGG ACTCCACAGG ATATTCTTGT GGACCAAAAG ACAAAATAGT 
ACAATTACAG GACCCTTTTT GCATGGTGTG AAGTGCTTCG AGGAGACCAG CATGGAGAAC 
AAAGTCACGC AGATCTCTCG AGAAGATCTG GAGGACCTGA GAGAAGCTTT CAACAAGATT 
GATATCGATA ACAGTGGCTA CGTGAGTGAT TTTGAGCTTC AAGAGCTCTT TCGTGAAGCC 
AGCTTTCACC TCCCGGGATA CCAGATACGG GAGATTGTAG AGAAGTTCAT TGCCGGTGAC 
ACCAACAAAG ATGAGAAGAT CAGTTTCGAA GAGTTTGTTG CCATATCCCA AGAGCTGAGA 
AGTAAAGAAG TGAGAGAAAC ATTCCGCAAG TGTATCTCCA GGCGGGATGG GATTCGGTCG 
TTTGGTGGGA CATCAGGTAT CTCGAGTGAA GGAACGCAGC ATTCCTACTC AGATGAAGAA 
AAGGTGGCCT TTGTGAACTG GGTCAATAAA GCTTTGGCAA ACGATCCCGA CTGCAAACAT 
CTGATCCCGA TGGACCCTGG GACTGACAGT TTATTCAAAT CTGTGAAAGA TGGAATTTTG 
CTGTGTAAAA TGATTAACCT GTCACAGCCA GACACCATCG ATGAGCGGGT CATCAACAAG 
AAATGCACTC CATTCACAAT GACTGAGAAT TTGATGTTGG CGCTCAACTC TGCTTCTGCG 
ATCGGCTGTA CGGTGGTCAA CATCGACGCG CAGGACATGA AAGCCGGGAC CCCTCACCTT 
GTGCTCGGCC TCTTGTGGCA AATCATAAAA ATCGGCCTCT TCGCTGACAT TGAGATCTCT 
CGCAACGAAG CGCTAATTGC TTTGCTGGAT GAGTCAGAAG AGCTGGACCA CCTTATGTCA 
ATGTCCCCTG AGGACCTTTT GCTTCGGTGG GTCAACTATC ACCTCAAAGC TGCTGGATGG 
AAACAGATCA GAAACTTCAG TGAAGATATC AAGGACTCCA GGGCGTATTT CCACCTGCTT 
AATCAGATCT CACCCAAAGG AGACTTGGAT GACCAGATGA GGATTGACAT TGACATGTTA 
GGACTCACCG AGCGTGATGA TGAGATGCGA GCAGAGCTGA TGTTGAGGCA GGCGGCTCGT 
CTGGACTGCA GACAGTTTGT CACTCCCAAG GACGTGGTTG CAGGAAACCA TAAGCTCAAC 
ATGGCGTTCG TGGCCAATCT TTTCAACATG TACCCAGCCT TGAACAGACC CACCGACAAC 
GGCATTGATT ATGCGATCGA GGGGGAAACG AGAGAGGACA GAACATTCAG GAACTGGATG 
AATTCTCTGG GGGTCTCTCC ATACGTTAAT CACCTGTACA GTGATCTGCA CGACGGCCTC 
ATCATTCTTC AGTTATATGA GAGAATCCAG ATACCGGTTG ACTGGCCTAG AGTCAACAAA 
CCTCCCTACC CTGTACTCGG CTCCAACATG AAGAAGCTTG AGAATTGCAA TTATGCGGTC 
GCACTGGGCA AAAAGGAAGC AAAGTTTTCC TTGGTAGGAA TCGGCGGTGA GAATATCAAT 
GAGGGAAGCC CCATGCACAC GCTGGCACTG GTCTGGCAAC TAATGAGAAG ATACACACTG 
AAGGTTCTGT CTGATCTTGG TGATGGGGAA AAAGTCAGTG ACCAAATAAT AATCAACTGG 
GTGAACAGCA CTCTGAAACA AGGCGATAAA AACTCCTCCA TTAGCAGCTT CAAGGACAAA 
TCGATCAGCA CAAGCCTGCC AGTGATTGAT CTGATCGACA CCATTGTGCC CAAAGCCATT 
AAATACGAGA TGGTGAAGAG CGGCGACATG ACGGCAGAGG ACAAGCTAAA CAATGCAAAA 
TATGCAATCT CTGTGGCGCG AAAAATAGGA GTCAAGGTGT ACGCCCTGCC CGATGACCTT 
GTGGAGGTCA AACCCAAGAT GGTGATGACG GTGTTCGCGT GCCTTATGGG AAGAGGCTTG 
AAGAAAGTTG ACGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334714.1
CDS	290..2287
Translation	MALITRGLHRIFLWTKRQNSTITGPFLHGVKCFEETSMENKVTQISREDLEDLREAFNKIDIDNSGYVSDFELQELFREASFHLPGYQIREIVEKFIAGDTNKDEKISFEEFVAISQELRSKEVRETFRKCISRRDGIRSFGGTSGISSEGTQHSYSDEEKVAFVNWVNKALANDPDCKHLIPMDPGTDSLFKSVKDGILLCKMINLSQPDTIDERVINKKCTPFTMTENLMLALNSASAIGCTVVNIDAQDMKAGTPHLVLGLLWQIIKIGLFADIEISRNEALIALLDESEELDHLMSMSPEDLLLRWVNYHLKAAGWKQIRNFSEDIKDSRAYFHLLNQISPKGDLDDQMRIDIDMLGLTERDDEMRAELMLRQAARLDCRQFVTPKDVVAGNHKLNMAFVANLFNMYPALNRPTDNGIDYAIEGETREDRTFRNWMNSLGVSPYVNHLYSDLHDGLIILQLYERIQIPVDWPRVNKPPYPVLGSNMKKLENCNYAVALGKKEAKFSLVGIGGENINEGSPMHTLALVWQLMRRYTLKVLSDLGDGEKVSDQIIINWVNSTLKQGDKNSSISSFKDKSISTSLPVIDLIDTIVPKAIKYEMVKSGDMTAEDKLNNAKYAISVARKIGVKVYALPDDLVEVKPKMVMTVFACLMGRGLKKVDG

Target ORF information:

RefSeq Version	XM_021479039.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio plastin 1 (I isoform) (pls1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479039.1

ORF Insert Sequence:

ATGGCGCTCA TCACCAGAGG ACTCCACAGG ATATTCTTGT GGACCAAAAG ACAAAATAGT 
ACAATTACAG GACCCTTTTT GCATGGTGTG AAGTGCTTCG AGGAGACCAG CATGGAGAAC 
AAAGTCACGC AGATCTCTCG AGAAGATCTG GAGGACCTGA GAGAAGCTTT CAACAAGATT 
GATATCGATA ACAGTGGCTA CGTGAGTGAT TTTGAGCTTC AAGAGCTCTT TCGTGAAGCC 
AGCTTTCACC TCCCGGGATA CCAGATACGG GAGATTGTAG AGAAGTTCAT TGCCGGTGAC 
ACCAACAAAG ATGAGAAGAT CAGTTTCGAA GAGTTTGTTG CCATATCCCA AGAGCTGAGA 
AGTAAAGAAG TGAGAGAAAC ATTCCGCAAG TGTATCTCCA GGCGGGATGG GATTCGGTCG 
TTTGGTGGGA CATCAGGTAT CTCGAGTGAA GGAACGCAGC ATTCCTACTC AGATGAAGAA 
AAGGTGGCCT TTGTGAACTG GGTCAATAAA GCTTTGGCAA ACGATCCCGA CTGCAAACAT 
CTGATCCCGA TGGACCCTGG GACTGACAGT TTATTCAAAT CTGTGAAAGA TGGAATTTTG 
CTGTGTAAAA TGATTAACCT GTCACAGCCA GACACCATCG ATGAGCGGGT CATCAACAAG 
AAATGCACTC CATTCACAAT GACTGAGAAT TTGATGTTGG CGCTCAACTC TGCTTCTGCG 
ATCGGCTGTA CGGTGGTCAA CATCGACGCG CAGGACATGA AAGCCGGGAC CCCTCACCTT 
GTGCTCGGCC TCTTGTGGCA AATCATAAAA ATCGGCCTCT TCGCTGACAT TGAGATCTCT 
CGCAACGAAG CGCTAATTGC TTTGCTGGAT GAGTCAGAAG AGCTGGACCA CCTTATGTCA 
ATGTCCCCTG AGGACCTTTT GCTTCGGTGG GTCAACTATC ACCTCAAAGC TGCTGGATGG 
AAACAGATCA GAAACTTCAG TGAAGATATC AAGGACTCCA GGGCGTATTT CCACCTGCTT 
AATCAGATCT CACCCAAAGG AGACTTGGAT GACCAGATGA GGATTGACAT TGACATGTTA 
GGACTCACCG AGCGTGATGA TGAGATGCGA GCAGAGCTGA TGTTGAGGCA GGCGGCTCGT 
CTGGACTGCA GACAGTTTGT CACTCCCAAG GACGTGGTTG CAGGAAACCA TAAGCTCAAC 
ATGGCGTTCG TGGCCAATCT TTTCAACATG TACCCAGCCT TGAACAGACC CACCGACAAC 
GGCATTGATT ATGCGATCGA GGGGGAAACG AGAGAGGACA GAACATTCAG GAACTGGATG 
AATTCTCTGG GGGTCTCTCC ATACGTTAAT CACCTGTACA GTGATCTGCA CGACGGCCTC 
ATCATTCTTC AGTTATATGA GAGAATCCAG ATACCGGTTG ACTGGCCTAG AGTCAACAAA 
CCTCCCTACC CTGTACTCGG CTCCAACATG AAGAAGCTTG AGAATTGCAA TTATGCGGTC 
GCACTGGGCA AAAAGGAAGC AAAGTTTTCC TTGGTAGGAA TCGGCGGTGA GAATATCAAT 
GAGGGAAGCC CCATGCACAC GCTGGCACTG GTCTGGCAAC TAATGAGAAG ATACACACTG 
AAGGTTCTGT CTGATCTTGG TGATGGGGAA AAAGTCAGTG ACCAAATAAT AATCAACTGG 
GTGAACAGCA CTCTGAAACA AGGCGATAAA AACTCCTCCA TTAGCAGCTT CAAGGACAAA 
TCGATCAGCA CAAGCCTGCC AGTGATTGAT CTGATCGACA CCATTGTGCC CAAAGCCATT 
AAATACGAGA TGGTGAAGAG CGGCGACATG ACGGCAGAGG ACAAGCTAAA CAATGCAAAA 
TATGCAATCT CTGTGGCGCG AAAAATAGGA GTCAAGGTGT ACGCCCTGCC CGATGACCTT 
GTGGAGGTCA AACCCAAGAT GGTGATGACG GTGTTCGCGT GCCTTATGGG AAGAGGCTTG 
AAGAAAGTTG ACGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Targeted deletion of the zebrafish actin-bundling protein L-plastin (lcp1).
PloS one13(1)e0190353(2018)
Kell MJ,Riccio RE,Baumgartner EA,Compton ZJ,Pecorin PJ,Mitchell TA,Topczewski J,LeClair EE

Sequential, Divergent, and Cooperative Requirements of
Genetics205(4)1551-1572(2017 04)
Yang YJ,Wang Y,Li Z,Zhou L,Gui JF

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Live imaging of chronic inflammation caused by mutation of zebrafish Hai1.
Journal of cell science120(Pt 19)3372-83(2007 Oct)
Mathias JR,Dodd ME,Walters KB,Rhodes J,Kanki JP,Look AT,Huttenlocher A

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pls1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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