Pls1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pls1
Entrez Gene ID	102502
Full Name	plastin 1 (I-isoform)
Synonyms	AI427122
General protein information	Preferred Names plastin 1 (I-isoform) Names plastin-1 I-fimbrin plastin 1, I
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 E3.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006510746.3	XP_006510809.1	plastin-1 isoform X1
XM_006510748.1	XP_006510811.1	plastin-1 isoform X2
XM_006510747.2	XP_006510810.1	plastin-1 isoform X1
XM_006510747.3	XP_006510810.1	plastin-1 isoform X1
NM_001033210.3	NP_001028382.1	plastin-1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0719100	NP_001047943.1
Bos taurus (cattle)	PLS1	NP_001096769.1
Danio rerio (zebrafish)	pls1	NP_956175.1
Gallus gallus (chicken)	PLS1	NP_990678.1
Pan troglodytes (chimpanzee)	PLS1	XP_001162185.1
Arabidopsis thaliana (thale cress)	FIM1	NP_001031726.1
Homo sapiens (human)	PLS1	NP_002661.2
	PLS1	XP_001112046.1
Canis lupus familiaris (dog)	PLS1	XP_542817.3
Mus musculus (house mouse)	Pls1	NP_001028382.1
Arabidopsis thaliana (thale cress)	AT5G55400	NP_200351.1
Xenopus tropicalis (tropical clawed frog)	pls1	NP_001123731.1

Gene Ontology
Bibliography

Related articles in PubMed

Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid.
Krey JF, Krystofiak ES, Dumont RA, Vijayakumar S, Choi D, Rivero F, Kachar B, Jones SM, Barr-Gillespie PG
The Journal of cell biology215(4)467-482(2016 Nov)

L-Plastin promotes podosome longevity and supports macrophage motility.
Zhou JY, Szasz TP, Stewart-Hutchinson PJ, Sivapalan J, Todd EM, Deady LE, Cooper JA, Onken MD, Morley SC
Molecular immunology7879-88(2016 10)

Absence of plastin 1 causes abnormal maintenance of hair cell stereocilia and a moderate form of hearing loss in mice.
Taylor R, Bullen A, Johnson SL, Grimm-G?nter EM, Rivero F, Marcotti W, Forge A, Daudet N
Human molecular genetics24(1)37-49(2015 Jan)

Expression analysis of mouse Rhobtb3 using a LacZ reporter and preliminary characterization of a knockout strain.
Lutz J, Grimm-G?nter EM, Joshi P, Rivero F
Histochemistry and cell biology142(5)511-28(2014 Nov)

L-plastin is essential for alveolar macrophage production and control of pulmonary pneumococcal infection.
Deady LE, Todd EM, Davis CG, Zhou JY, Topcagic N, Edelson BT, Ferkol TW, Cooper MA, Muenzer JT, Morley SC
Infection and immunity82(5)1982-93(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PLS1 biases actin toward liquid packing, which allows stereocilia to grow to a greater diameter.
Title: Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid.

we have identified a role for L-plastin in stabilizing long-lived podosomes and in enabling macrophage motility
Title: L-Plastin promotes podosome longevity and supports macrophage motility.

Results show that plastin 1 is dispensable for the initial formation of stereocilia.
Title: Absence of plastin 1 causes abnormal maintenance of hair cell stereocilia and a moderate form of hearing loss in mice.

This study identified LPL as being key to alveolar macrophage development and essential to an effective antipneumococcal response.
Title: L-plastin is essential for alveolar macrophage production and control of pulmonary pneumococcal infection.

Plastin 1 accumulates in the terminal web and interacts with keratin 19, possibly contributing to anchoring the rootlets to the keratin network.
Title: Plastin 1 binds to keratin and is required for terminal web assembly in the intestinal epithelium.

The following Pls1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pls1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12491	NM_001033210.3 Latest version!	Mus musculus plastin 1 (I-isoform) (Pls1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu12491	XM_006510746.3 Latest version!	Mus musculus plastin 1 (I-isoform) (Pls1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu42494	XM_006510748.1 Latest version!	Mus musculus plastin 1 (I-isoform) (Pls1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu12491	XM_006510747.3 Latest version!	Mus musculus plastin 1 (I-isoform) (Pls1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu12491	XM_006510747.2	Mus musculus plastin 1 (I-isoform) (Pls1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pls1 ( NM_001033210.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028382.1 Mus musculus plastin 1 (I-isoform) (Pls1), mRNA.

Price & Availability↑

CloneID	OMu12491
Clone ID Related Accession (Same CDS sequence)	XM_006510746.3 , XM_006510747.2 , XM_006510747.3 , NM_001033210.3
Accession Version	NM_001033210.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	plastin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AI526519.1, AK133070.1, AW124763.1, AK030875.1 and CJ229558.1. On Oct 23, 2009 this sequence version replaced NM_001033210.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.92981.1, AK133070.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGAAAACA GTACCACCAC CATTTCTCGG GAGGAGCTTG AAGAGCTACA AGAAGCGTTT 
AATAAAATAG ATATTGACAA CAGTGGATAT GTTAGTGACT ATGAACTTCA GGACCTATTT 
AAGGAGGCAA GCCTTCCCCT TCCTGGGTAC AAGGTGCGCG AGATTGTGGA GAAAATATTA 
GTAGTTGCCG ACAACAACAA AGATGGTAAA ATCAGTTTTG AGGAGTTCGT GTCGCTGATG 
CAAGAGCTAA AAAGCAAAGA TATCAGCAAA ACGTTTCGAA AAATAATTAA CAAAAGGGAA 
GGGATCACTG CCATCGGGGG GACGTCATCC ATCTCCAGCG AGGGCACCCA GCATTCGTAC 
TCAGAGGAAG AAAAAGTGGC TTTTGTGAAC TGGATAAACA AAGCTCTGGA GAATGATGCA 
GACTGTAGCC ACCTCCTACC CATGAACCCC AACGACGGCA GCCTTTTCAA GTCGCTGGCA 
GATGGCATCC TTCTCTGCAA AATGATCAAC TTATCCGAGC CAGATACGAT TGACGAGAGA 
GCCATCAATA AGAAGAAGCT CACTCCGTTC ACGGTGTCGG AAAATCTGAA CCTAGCACTG 
AACTCTGCCT CGGCCATCGG TTGCACCGTG GTCAACATTG GTGCTCAGGA CCTCAAGGAA 
GGAAAGCCTC ACTTGGTCTT GGGGCTTCTC TGGCAGATCA TCAAAGTGGG TCTTTTTGCT 
GATATCGAGA TTTCCAGGAA TGAAGCCTTG ATCGCGTTGC TAAAAGATGG CGAGGACCTG 
GAGGAGCTGA TGAAGCTCTC TCCTGAGGAA CTGCTGCTCC GATGGGTGAA CTACCATCTG 
ACCAATGCGG GCTGGCGCAC CATCAACAAC TTCAGCCAGG ACATCAAGGA TTCAAAAGCC 
TATTTCCACC TTCTTAATCA GATTGCTCCT AAAGGCGACC GAGATGATGG GCCTGCCGTA 
GCCATTGATC TTTCAGGATT TAATGAGAAA AACGACCTGA AGCGTGCTGG ATTTATGCTC 
CAGGAAGCGG ATAAACTTGG CTGCAGACAG TTTGTCACTC CTGCAGATGT GGTTTCAGGG 
AACCCTAAGC TAAACTTAGC CTTTGTAGCG AATCTTTTTA ACACATACCC ATGCCTACAC 
AAGCCTGACA ATAATGACAT TGATCTCAAC TTACTGGAAG GCGAGAGCAA GGAGGAGAGG 
ACATTCCGCA ACTGGATGAA CTCGCTGGGC GTGAACCCGT ACATCAATCA CCTGTACAGT 
GACCTTGCAG ACGCTTTGGT GATCTTTCAG CTCTACGAGA TGATCCGCGT GCCAGTCAAC 
TGGAGCCAGG TGAACAAGCC TCCGTACCCT GCTCTGGGAG GAAACATGAA GAAGATTGAA 
AACTGTAACT ACGCGGTGGA GCTTGGGAAG AATGAGGCCA AGTTCTCTTT GGTTGGCATC 
GCCGGGCAGG ACTTAAATGA AGGGAACGCA ACACTTACCC TGGCACTGGT GTGGCAGTTG 
ATGAGAAGAT ACACGCTGAA AGTGTTATCA GACCTTGGAG AAGGTGAAAA AGTCACTGAT 
GATATCATTA TTAAATGGGT CAACCAGACC CTTAAAAGTG CAAACAAGAG CACTTCTATT 
TCCAGCTTCA AGGATAAATC TATTAGTACA AGTTTGCCTG TCTTAGATCT AATAGATGCC 
ATTGCACCAA ATGCAGTCCG TCAAGAAATG ATCAAGAGAG AGCACTTGAC TGATGAGGAC 
AAACTGAACA ATGCTAAATA CGCCATTTCA GTTGCTCGGA AGATCGGTGC CCGGATATAC 
GCGCTGCCTG ACGACCTCGT GGAAGTGAAG CCGAAGATGG TGATGACTGT GTTTGCCTGC 
TTAATGGGGA AAGGATTGAA CAGACTGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028382.1
CDS	145..2037
Translation	MENSTTTISREELEELQEAFNKIDIDNSGYVSDYELQDLFKEASLPLPGYKVREIVEKILVVADNNKDGKISFEEFVSLMQELKSKDISKTFRKIINKREGITAIGGTSSISSEGTQHSYSEEEKVAFVNWINKALENDADCSHLLPMNPNDGSLFKSLADGILLCKMINLSEPDTIDERAINKKKLTPFTVSENLNLALNSASAIGCTVVNIGAQDLKEGKPHLVLGLLWQIIKVGLFADIEISRNEALIALLKDGEDLEELMKLSPEELLLRWVNYHLTNAGWRTINNFSQDIKDSKAYFHLLNQIAPKGDRDDGPAVAIDLSGFNEKNDLKRAGFMLQEADKLGCRQFVTPADVVSGNPKLNLAFVANLFNTYPCLHKPDNNDIDLNLLEGESKEERTFRNWMNSLGVNPYINHLYSDLADALVIFQLYEMIRVPVNWSQVNKPPYPALGGNMKKIENCNYAVELGKNEAKFSLVGIAGQDLNEGNATLTLALVWQLMRRYTLKVLSDLGEGEKVTDDIIIKWVNQTLKSANKSTSISSFKDKSISTSLPVLDLIDAIAPNAVRQEMIKREHLTDEDKLNNAKYAISVARKIGARIYALPDDLVEVKPKMVMTVFACLMGKGLNRLK

Target ORF information:

RefSeq Version	NM_001033210.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus plastin 1 (I-isoform) (Pls1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033210.3

ORF Insert Sequence:

ATGGAAAACA GTACCACCAC CATTTCTCGG GAGGAGCTTG AAGAGCTACA AGAAGCGTTT 
AATAAAATAG ATATTGACAA CAGTGGATAT GTTAGTGACT ATGAACTTCA GGACCTATTT 
AAGGAGGCAA GCCTTCCCCT TCCTGGGTAC AAGGTGCGCG AGATTGTGGA GAAAATATTA 
GTAGTTGCCG ACAACAACAA AGATGGTAAA ATCAGTTTTG AGGAGTTCGT GTCGCTGATG 
CAAGAGCTAA AAAGCAAAGA TATCAGCAAA ACGTTTCGAA AAATAATTAA CAAAAGGGAA 
GGGATCACTG CCATCGGGGG GACGTCATCC ATCTCCAGCG AGGGCACCCA GCATTCGTAC 
TCAGAGGAAG AAAAAGTGGC TTTTGTGAAC TGGATAAACA AAGCTCTGGA GAATGATGCA 
GACTGTAGCC ACCTCCTACC CATGAACCCC AACGACGGCA GCCTTTTCAA GTCGCTGGCA 
GATGGCATCC TTCTCTGCAA AATGATCAAC TTATCCGAGC CAGATACGAT TGACGAGAGA 
GCCATCAATA AGAAGAAGCT CACTCCGTTC ACGGTGTCGG AAAATCTGAA CCTAGCACTG 
AACTCTGCCT CGGCCATCGG TTGCACCGTG GTCAACATTG GTGCTCAGGA CCTCAAGGAA 
GGAAAGCCTC ACTTGGTCTT GGGGCTTCTC TGGCAGATCA TCAAAGTGGG TCTTTTTGCT 
GATATCGAGA TTTCCAGGAA TGAAGCCTTG ATCGCGTTGC TAAAAGATGG CGAGGACCTG 
GAGGAGCTGA TGAAGCTCTC TCCTGAGGAA CTGCTGCTCC GATGGGTGAA CTACCATCTG 
ACCAATGCGG GCTGGCGCAC CATCAACAAC TTCAGCCAGG ACATCAAGGA TTCAAAAGCC 
TATTTCCACC TTCTTAATCA GATTGCTCCT AAAGGCGACC GAGATGATGG GCCTGCCGTA 
GCCATTGATC TTTCAGGATT TAATGAGAAA AACGACCTGA AGCGTGCTGG ATTTATGCTC 
CAGGAAGCGG ATAAACTTGG CTGCAGACAG TTTGTCACTC CTGCAGATGT GGTTTCAGGG 
AACCCTAAGC TAAACTTAGC CTTTGTAGCG AATCTTTTTA ACACATACCC ATGCCTACAC 
AAGCCTGACA ATAATGACAT TGATCTCAAC TTACTGGAAG GCGAGAGCAA GGAGGAGAGG 
ACATTCCGCA ACTGGATGAA CTCGCTGGGC GTGAACCCGT ACATCAATCA CCTGTACAGT 
GACCTTGCAG ACGCTTTGGT GATCTTTCAG CTCTACGAGA TGATCCGCGT GCCAGTCAAC 
TGGAGCCAGG TGAACAAGCC TCCGTACCCT GCTCTGGGAG GAAACATGAA GAAGATTGAA 
AACTGTAACT ACGCGGTGGA GCTTGGGAAG AATGAGGCCA AGTTCTCTTT GGTTGGCATC 
GCCGGGCAGG ACTTAAATGA AGGGAACGCA ACACTTACCC TGGCACTGGT GTGGCAGTTG 
ATGAGAAGAT ACACGCTGAA AGTGTTATCA GACCTTGGAG AAGGTGAAAA AGTCACTGAT 
GATATCATTA TTAAATGGGT CAACCAGACC CTTAAAAGTG CAAACAAGAG CACTTCTATT 
TCCAGCTTCA AGGATAAATC TATTAGTACA AGTTTGCCTG TCTTAGATCT AATAGATGCC 
ATTGCACCAA ATGCAGTCCG TCAAGAAATG ATCAAGAGAG AGCACTTGAC TGATGAGGAC 
AAACTGAACA ATGCTAAATA CGCCATTTCA GTTGCTCGGA AGATCGGTGC CCGGATATAC 
GCGCTGCCTG ACGACCTCGT GGAAGTGAAG CCGAAGATGG TGATGACTGT GTTTGCCTGC 
TTAATGGGGA AAGGATTGAA CAGACTGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pls1 ( XM_006510746.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510809.1 Mus musculus plastin 1 (I-isoform) (Pls1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu12491
Clone ID Related Accession (Same CDS sequence)	XM_006510746.3 , XM_006510747.2 , XM_006510747.3 , NM_001033210.3
Accession Version	XM_006510746.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	plastin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510746.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACA GTACCACCAC CATTTCTCGG GAGGAGCTTG AAGAGCTACA AGAAGCGTTT 
AATAAAATAG ATATTGACAA CAGTGGATAT GTTAGTGACT ATGAACTTCA GGACCTATTT 
AAGGAGGCAA GCCTTCCCCT TCCTGGGTAC AAGGTGCGCG AGATTGTGGA GAAAATATTA 
GTAGTTGCCG ACAACAACAA AGATGGTAAA ATCAGTTTTG AGGAGTTCGT GTCGCTGATG 
CAAGAGCTAA AAAGCAAAGA TATCAGCAAA ACGTTTCGAA AAATAATTAA CAAAAGGGAA 
GGGATCACTG CCATCGGGGG GACGTCATCC ATCTCCAGCG AGGGCACCCA GCATTCGTAC 
TCAGAGGAAG AAAAAGTGGC TTTTGTGAAC TGGATAAACA AAGCTCTGGA GAATGATGCA 
GACTGTAGCC ACCTCCTACC CATGAACCCC AACGACGGCA GCCTTTTCAA GTCGCTGGCA 
GATGGCATCC TTCTCTGCAA AATGATCAAC TTATCCGAGC CAGATACGAT TGACGAGAGA 
GCCATCAATA AGAAGAAGCT CACTCCGTTC ACGGTGTCGG AAAATCTGAA CCTAGCACTG 
AACTCTGCCT CGGCCATCGG TTGCACCGTG GTCAACATTG GTGCTCAGGA CCTCAAGGAA 
GGAAAGCCTC ACTTGGTCTT GGGGCTTCTC TGGCAGATCA TCAAAGTGGG TCTTTTTGCT 
GATATCGAGA TTTCCAGGAA TGAAGCCTTG ATCGCGTTGC TAAAAGATGG CGAGGACCTG 
GAGGAGCTGA TGAAGCTCTC TCCTGAGGAA CTGCTGCTCC GATGGGTGAA CTACCATCTG 
ACCAATGCGG GCTGGCGCAC CATCAACAAC TTCAGCCAGG ACATCAAGGA TTCAAAAGCC 
TATTTCCACC TTCTTAATCA GATTGCTCCT AAAGGCGACC GAGATGATGG GCCTGCCGTA 
GCCATTGATC TTTCAGGATT TAATGAGAAA AACGACCTGA AGCGTGCTGG ATTTATGCTC 
CAGGAAGCGG ATAAACTTGG CTGCAGACAG TTTGTCACTC CTGCAGATGT GGTTTCAGGG 
AACCCTAAGC TAAACTTAGC CTTTGTAGCG AATCTTTTTA ACACATACCC ATGCCTACAC 
AAGCCTGACA ATAATGACAT TGATCTCAAC TTACTGGAAG GCGAGAGCAA GGAGGAGAGG 
ACATTCCGCA ACTGGATGAA CTCGCTGGGC GTGAACCCGT ACATCAATCA CCTGTACAGT 
GACCTTGCAG ACGCTTTGGT GATCTTTCAG CTCTACGAGA TGATCCGCGT GCCAGTCAAC 
TGGAGCCAGG TGAACAAGCC TCCGTACCCT GCTCTGGGAG GAAACATGAA GAAGATTGAA 
AACTGTAACT ACGCGGTGGA GCTTGGGAAG AATGAGGCCA AGTTCTCTTT GGTTGGCATC 
GCCGGGCAGG ACTTAAATGA AGGGAACGCA ACACTTACCC TGGCACTGGT GTGGCAGTTG 
ATGAGAAGAT ACACGCTGAA AGTGTTATCA GACCTTGGAG AAGGTGAAAA AGTCACTGAT 
GATATCATTA TTAAATGGGT CAACCAGACC CTTAAAAGTG CAAACAAGAG CACTTCTATT 
TCCAGCTTCA AGGATAAATC TATTAGTACA AGTTTGCCTG TCTTAGATCT AATAGATGCC 
ATTGCACCAA ATGCAGTCCG TCAAGAAATG ATCAAGAGAG AGCACTTGAC TGATGAGGAC 
AAACTGAACA ATGCTAAATA CGCCATTTCA GTTGCTCGGA AGATCGGTGC CCGGATATAC 
GCGCTGCCTG ACGACCTCGT GGAAGTGAAG CCGAAGATGG TGATGACTGT GTTTGCCTGC 
TTAATGGGGA AAGGATTGAA CAGACTGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510809.1
CDS	222..2114
Translation	MENSTTTISREELEELQEAFNKIDIDNSGYVSDYELQDLFKEASLPLPGYKVREIVEKILVVADNNKDGKISFEEFVSLMQELKSKDISKTFRKIINKREGITAIGGTSSISSEGTQHSYSEEEKVAFVNWINKALENDADCSHLLPMNPNDGSLFKSLADGILLCKMINLSEPDTIDERAINKKKLTPFTVSENLNLALNSASAIGCTVVNIGAQDLKEGKPHLVLGLLWQIIKVGLFADIEISRNEALIALLKDGEDLEELMKLSPEELLLRWVNYHLTNAGWRTINNFSQDIKDSKAYFHLLNQIAPKGDRDDGPAVAIDLSGFNEKNDLKRAGFMLQEADKLGCRQFVTPADVVSGNPKLNLAFVANLFNTYPCLHKPDNNDIDLNLLEGESKEERTFRNWMNSLGVNPYINHLYSDLADALVIFQLYEMIRVPVNWSQVNKPPYPALGGNMKKIENCNYAVELGKNEAKFSLVGIAGQDLNEGNATLTLALVWQLMRRYTLKVLSDLGEGEKVTDDIIIKWVNQTLKSANKSTSISSFKDKSISTSLPVLDLIDAIAPNAVRQEMIKREHLTDEDKLNNAKYAISVARKIGARIYALPDDLVEVKPKMVMTVFACLMGKGLNRLK

Target ORF information:

RefSeq Version	XM_006510746.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus plastin 1 (I-isoform) (Pls1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510746.3

ORF Insert Sequence:

ATGGAAAACA GTACCACCAC CATTTCTCGG GAGGAGCTTG AAGAGCTACA AGAAGCGTTT 
AATAAAATAG ATATTGACAA CAGTGGATAT GTTAGTGACT ATGAACTTCA GGACCTATTT 
AAGGAGGCAA GCCTTCCCCT TCCTGGGTAC AAGGTGCGCG AGATTGTGGA GAAAATATTA 
GTAGTTGCCG ACAACAACAA AGATGGTAAA ATCAGTTTTG AGGAGTTCGT GTCGCTGATG 
CAAGAGCTAA AAAGCAAAGA TATCAGCAAA ACGTTTCGAA AAATAATTAA CAAAAGGGAA 
GGGATCACTG CCATCGGGGG GACGTCATCC ATCTCCAGCG AGGGCACCCA GCATTCGTAC 
TCAGAGGAAG AAAAAGTGGC TTTTGTGAAC TGGATAAACA AAGCTCTGGA GAATGATGCA 
GACTGTAGCC ACCTCCTACC CATGAACCCC AACGACGGCA GCCTTTTCAA GTCGCTGGCA 
GATGGCATCC TTCTCTGCAA AATGATCAAC TTATCCGAGC CAGATACGAT TGACGAGAGA 
GCCATCAATA AGAAGAAGCT CACTCCGTTC ACGGTGTCGG AAAATCTGAA CCTAGCACTG 
AACTCTGCCT CGGCCATCGG TTGCACCGTG GTCAACATTG GTGCTCAGGA CCTCAAGGAA 
GGAAAGCCTC ACTTGGTCTT GGGGCTTCTC TGGCAGATCA TCAAAGTGGG TCTTTTTGCT 
GATATCGAGA TTTCCAGGAA TGAAGCCTTG ATCGCGTTGC TAAAAGATGG CGAGGACCTG 
GAGGAGCTGA TGAAGCTCTC TCCTGAGGAA CTGCTGCTCC GATGGGTGAA CTACCATCTG 
ACCAATGCGG GCTGGCGCAC CATCAACAAC TTCAGCCAGG ACATCAAGGA TTCAAAAGCC 
TATTTCCACC TTCTTAATCA GATTGCTCCT AAAGGCGACC GAGATGATGG GCCTGCCGTA 
GCCATTGATC TTTCAGGATT TAATGAGAAA AACGACCTGA AGCGTGCTGG ATTTATGCTC 
CAGGAAGCGG ATAAACTTGG CTGCAGACAG TTTGTCACTC CTGCAGATGT GGTTTCAGGG 
AACCCTAAGC TAAACTTAGC CTTTGTAGCG AATCTTTTTA ACACATACCC ATGCCTACAC 
AAGCCTGACA ATAATGACAT TGATCTCAAC TTACTGGAAG GCGAGAGCAA GGAGGAGAGG 
ACATTCCGCA ACTGGATGAA CTCGCTGGGC GTGAACCCGT ACATCAATCA CCTGTACAGT 
GACCTTGCAG ACGCTTTGGT GATCTTTCAG CTCTACGAGA TGATCCGCGT GCCAGTCAAC 
TGGAGCCAGG TGAACAAGCC TCCGTACCCT GCTCTGGGAG GAAACATGAA GAAGATTGAA 
AACTGTAACT ACGCGGTGGA GCTTGGGAAG AATGAGGCCA AGTTCTCTTT GGTTGGCATC 
GCCGGGCAGG ACTTAAATGA AGGGAACGCA ACACTTACCC TGGCACTGGT GTGGCAGTTG 
ATGAGAAGAT ACACGCTGAA AGTGTTATCA GACCTTGGAG AAGGTGAAAA AGTCACTGAT 
GATATCATTA TTAAATGGGT CAACCAGACC CTTAAAAGTG CAAACAAGAG CACTTCTATT 
TCCAGCTTCA AGGATAAATC TATTAGTACA AGTTTGCCTG TCTTAGATCT AATAGATGCC 
ATTGCACCAA ATGCAGTCCG TCAAGAAATG ATCAAGAGAG AGCACTTGAC TGATGAGGAC 
AAACTGAACA ATGCTAAATA CGCCATTTCA GTTGCTCGGA AGATCGGTGC CCGGATATAC 
GCGCTGCCTG ACGACCTCGT GGAAGTGAAG CCGAAGATGG TGATGACTGT GTTTGCCTGC 
TTAATGGGGA AAGGATTGAA CAGACTGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pls1 ( XM_006510747.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510810.1 Mus musculus plastin 1 (I-isoform) (Pls1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu12491
Clone ID Related Accession (Same CDS sequence)	XM_006510746.3 , XM_006510747.2 , XM_006510747.3 , NM_001033210.3
Accession Version	XM_006510747.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	plastin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACA GTACCACCAC CATTTCTCGG GAGGAGCTTG AAGAGCTACA AGAAGCGTTT 
AATAAAATAG ATATTGACAA CAGTGGATAT GTTAGTGACT ATGAACTTCA GGACCTATTT 
AAGGAGGCAA GCCTTCCCCT TCCTGGGTAC AAGGTGCGCG AGATTGTGGA GAAAATATTA 
GTAGTTGCCG ACAACAACAA AGATGGTAAA ATCAGTTTTG AGGAGTTCGT GTCGCTGATG 
CAAGAGCTAA AAAGCAAAGA TATCAGCAAA ACGTTTCGAA AAATAATTAA CAAAAGGGAA 
GGGATCACTG CCATCGGGGG GACGTCATCC ATCTCCAGCG AGGGCACCCA GCATTCGTAC 
TCAGAGGAAG AAAAAGTGGC TTTTGTGAAC TGGATAAACA AAGCTCTGGA GAATGATGCA 
GACTGTAGCC ACCTCCTACC CATGAACCCC AACGACGGCA GCCTTTTCAA GTCGCTGGCA 
GATGGCATCC TTCTCTGCAA AATGATCAAC TTATCCGAGC CAGATACGAT TGACGAGAGA 
GCCATCAATA AGAAGAAGCT CACTCCGTTC ACGGTGTCGG AAAATCTGAA CCTAGCACTG 
AACTCTGCCT CGGCCATCGG TTGCACCGTG GTCAACATTG GTGCTCAGGA CCTCAAGGAA 
GGAAAGCCTC ACTTGGTCTT GGGGCTTCTC TGGCAGATCA TCAAAGTGGG TCTTTTTGCT 
GATATCGAGA TTTCCAGGAA TGAAGCCTTG ATCGCGTTGC TAAAAGATGG CGAGGACCTG 
GAGGAGCTGA TGAAGCTCTC TCCTGAGGAA CTGCTGCTCC GATGGGTGAA CTACCATCTG 
ACCAATGCGG GCTGGCGCAC CATCAACAAC TTCAGCCAGG ACATCAAGGA TTCAAAAGCC 
TATTTCCACC TTCTTAATCA GATTGCTCCT AAAGGCGACC GAGATGATGG GCCTGCCGTA 
GCCATTGATC TTTCAGGATT TAATGAGAAA AACGACCTGA AGCGTGCTGG ATTTATGCTC 
CAGGAAGCGG ATAAACTTGG CTGCAGACAG TTTGTCACTC CTGCAGATGT GGTTTCAGGG 
AACCCTAAGC TAAACTTAGC CTTTGTAGCG AATCTTTTTA ACACATACCC ATGCCTACAC 
AAGCCTGACA ATAATGACAT TGATCTCAAC TTACTGGAAG GCGAGAGCAA GGAGGAGAGG 
ACATTCCGCA ACTGGATGAA CTCGCTGGGC GTGAACCCGT ACATCAATCA CCTGTACAGT 
GACCTTGCAG ACGCTTTGGT GATCTTTCAG CTCTACGAGA TGATCCGCGT GCCAGTCAAC 
TGGAGCCAGG TGAACAAGCC TCCGTACCCT GCTCTGGGAG GAAACATGAA GAAGATTGAA 
AACTGTAACT ACGCGGTGGA GCTTGGGAAG AATGAGGCCA AGTTCTCTTT GGTTGGCATC 
GCCGGGCAGG ACTTAAATGA AGGGAACGCA ACACTTACCC TGGCACTGGT GTGGCAGTTG 
ATGAGAAGAT ACACGCTGAA AGTGTTATCA GACCTTGGAG AAGGTGAAAA AGTCACTGAT 
GATATCATTA TTAAATGGGT CAACCAGACC CTTAAAAGTG CAAACAAGAG CACTTCTATT 
TCCAGCTTCA AGGATAAATC TATTAGTACA AGTTTGCCTG TCTTAGATCT AATAGATGCC 
ATTGCACCAA ATGCAGTCCG TCAAGAAATG ATCAAGAGAG AGCACTTGAC TGATGAGGAC 
AAACTGAACA ATGCTAAATA CGCCATTTCA GTTGCTCGGA AGATCGGTGC CCGGATATAC 
GCGCTGCCTG ACGACCTCGT GGAAGTGAAG CCGAAGATGG TGATGACTGT GTTTGCCTGC 
TTAATGGGGA AAGGATTGAA CAGACTGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510810.1
CDS	57..1949
Translation	MENSTTTISREELEELQEAFNKIDIDNSGYVSDYELQDLFKEASLPLPGYKVREIVEKILVVADNNKDGKISFEEFVSLMQELKSKDISKTFRKIINKREGITAIGGTSSISSEGTQHSYSEEEKVAFVNWINKALENDADCSHLLPMNPNDGSLFKSLADGILLCKMINLSEPDTIDERAINKKKLTPFTVSENLNLALNSASAIGCTVVNIGAQDLKEGKPHLVLGLLWQIIKVGLFADIEISRNEALIALLKDGEDLEELMKLSPEELLLRWVNYHLTNAGWRTINNFSQDIKDSKAYFHLLNQIAPKGDRDDGPAVAIDLSGFNEKNDLKRAGFMLQEADKLGCRQFVTPADVVSGNPKLNLAFVANLFNTYPCLHKPDNNDIDLNLLEGESKEERTFRNWMNSLGVNPYINHLYSDLADALVIFQLYEMIRVPVNWSQVNKPPYPALGGNMKKIENCNYAVELGKNEAKFSLVGIAGQDLNEGNATLTLALVWQLMRRYTLKVLSDLGEGEKVTDDIIIKWVNQTLKSANKSTSISSFKDKSISTSLPVLDLIDAIAPNAVRQEMIKREHLTDEDKLNNAKYAISVARKIGARIYALPDDLVEVKPKMVMTVFACLMGKGLNRLK

Target ORF information:

RefSeq Version	XM_006510747.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus plastin 1 (I-isoform) (Pls1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510747.2

ORF Insert Sequence:

ATGGAAAACA GTACCACCAC CATTTCTCGG GAGGAGCTTG AAGAGCTACA AGAAGCGTTT 
AATAAAATAG ATATTGACAA CAGTGGATAT GTTAGTGACT ATGAACTTCA GGACCTATTT 
AAGGAGGCAA GCCTTCCCCT TCCTGGGTAC AAGGTGCGCG AGATTGTGGA GAAAATATTA 
GTAGTTGCCG ACAACAACAA AGATGGTAAA ATCAGTTTTG AGGAGTTCGT GTCGCTGATG 
CAAGAGCTAA AAAGCAAAGA TATCAGCAAA ACGTTTCGAA AAATAATTAA CAAAAGGGAA 
GGGATCACTG CCATCGGGGG GACGTCATCC ATCTCCAGCG AGGGCACCCA GCATTCGTAC 
TCAGAGGAAG AAAAAGTGGC TTTTGTGAAC TGGATAAACA AAGCTCTGGA GAATGATGCA 
GACTGTAGCC ACCTCCTACC CATGAACCCC AACGACGGCA GCCTTTTCAA GTCGCTGGCA 
GATGGCATCC TTCTCTGCAA AATGATCAAC TTATCCGAGC CAGATACGAT TGACGAGAGA 
GCCATCAATA AGAAGAAGCT CACTCCGTTC ACGGTGTCGG AAAATCTGAA CCTAGCACTG 
AACTCTGCCT CGGCCATCGG TTGCACCGTG GTCAACATTG GTGCTCAGGA CCTCAAGGAA 
GGAAAGCCTC ACTTGGTCTT GGGGCTTCTC TGGCAGATCA TCAAAGTGGG TCTTTTTGCT 
GATATCGAGA TTTCCAGGAA TGAAGCCTTG ATCGCGTTGC TAAAAGATGG CGAGGACCTG 
GAGGAGCTGA TGAAGCTCTC TCCTGAGGAA CTGCTGCTCC GATGGGTGAA CTACCATCTG 
ACCAATGCGG GCTGGCGCAC CATCAACAAC TTCAGCCAGG ACATCAAGGA TTCAAAAGCC 
TATTTCCACC TTCTTAATCA GATTGCTCCT AAAGGCGACC GAGATGATGG GCCTGCCGTA 
GCCATTGATC TTTCAGGATT TAATGAGAAA AACGACCTGA AGCGTGCTGG ATTTATGCTC 
CAGGAAGCGG ATAAACTTGG CTGCAGACAG TTTGTCACTC CTGCAGATGT GGTTTCAGGG 
AACCCTAAGC TAAACTTAGC CTTTGTAGCG AATCTTTTTA ACACATACCC ATGCCTACAC 
AAGCCTGACA ATAATGACAT TGATCTCAAC TTACTGGAAG GCGAGAGCAA GGAGGAGAGG 
ACATTCCGCA ACTGGATGAA CTCGCTGGGC GTGAACCCGT ACATCAATCA CCTGTACAGT 
GACCTTGCAG ACGCTTTGGT GATCTTTCAG CTCTACGAGA TGATCCGCGT GCCAGTCAAC 
TGGAGCCAGG TGAACAAGCC TCCGTACCCT GCTCTGGGAG GAAACATGAA GAAGATTGAA 
AACTGTAACT ACGCGGTGGA GCTTGGGAAG AATGAGGCCA AGTTCTCTTT GGTTGGCATC 
GCCGGGCAGG ACTTAAATGA AGGGAACGCA ACACTTACCC TGGCACTGGT GTGGCAGTTG 
ATGAGAAGAT ACACGCTGAA AGTGTTATCA GACCTTGGAG AAGGTGAAAA AGTCACTGAT 
GATATCATTA TTAAATGGGT CAACCAGACC CTTAAAAGTG CAAACAAGAG CACTTCTATT 
TCCAGCTTCA AGGATAAATC TATTAGTACA AGTTTGCCTG TCTTAGATCT AATAGATGCC 
ATTGCACCAA ATGCAGTCCG TCAAGAAATG ATCAAGAGAG AGCACTTGAC TGATGAGGAC 
AAACTGAACA ATGCTAAATA CGCCATTTCA GTTGCTCGGA AGATCGGTGC CCGGATATAC 
GCGCTGCCTG ACGACCTCGT GGAAGTGAAG CCGAAGATGG TGATGACTGT GTTTGCCTGC 
TTAATGGGGA AAGGATTGAA CAGACTGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pls1 ( XM_006510748.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510811.1 Mus musculus plastin 1 (I-isoform) (Pls1), transcript variant X3, mRNA.

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CloneID	OMu42494
Clone ID Related Accession (Same CDS sequence)	XM_006510748.1
Accession Version	XM_006510748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	plastin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGC TAAAAAGCAA AGATATCAGC AAAACGTTTC GAAAAATAAT TAACAAAAGG 
GAAGGGATCA CTGCCATCGG GGGGACGTCA TCCATCTCCA GCGAGGGCAC CCAGCATTCG 
TACTCAGAGG AAGAAAAAGT GGCTTTTGTG AACTGGATAA ACAAAGCTCT GGAGAATGAT 
GCAGACTGTA GCCACCTCCT ACCCATGAAC CCCAACGACG GCAGCCTTTT CAAGTCGCTG 
GCAGATGGCA TCCTTCTCTG CAAAATGATC AACTTATCCG AGCCAGATAC GATTGACGAG 
AGAGCCATCA ATAAGAAGAA GCTCACTCCG TTCACGGTGT CGGAAAATCT GAACCTAGCA 
CTGAACTCTG CCTCGGCCAT CGGTTGCACC GTGGTCAACA TTGGTGCTCA GGACCTCAAG 
GAAGGAAAGC CTCACTTGGT CTTGGGGCTT CTCTGGCAGA TCATCAAAGT GGGTCTTTTT 
GCTGATATCG AGATTTCCAG GAATGAAGCC TTGATCGCGT TGCTAAAAGA TGGCGAGGAC 
CTGGAGGAGC TGATGAAGCT CTCTCCTGAG GAACTGCTGC TCCGATGGGT GAACTACCAT 
CTGACCAATG CGGGCTGGCG CACCATCAAC AACTTCAGCC AGGACATCAA GGATTCAAAA 
GCCTATTTCC ACCTTCTTAA TCAGATTGCT CCTAAAGGCG ACCGAGATGA TGGGCCTGCC 
GTAGCCATTG ATCTTTCAGG ATTTAATGAG AAAAACGACC TGAAGCGTGC TGGATTTATG 
CTCCAGGAAG CGGATAAACT TGGCTGCAGA CAGTTTGTCA CTCCTGCAGA TGTGGTTTCA 
GGGAACCCTA AGCTAAACTT AGCCTTTGTA GCGAATCTTT TTAACACATA CCCATGCCTA 
CACAAGCCTG ACAATAATGA CATTGATCTC AACTTACTGG AAGGCGAGAG CAAGGAGGAG 
AGGACATTCC GCAACTGGAT GAACTCGCTG GGCGTGAACC CGTACATCAA TCACCTGTAC 
AGTGACCTTG CAGACGCTTT GGTGATCTTT CAGCTCTACG AGATGATCCG CGTGCCAGTC 
AACTGGAGCC AGGTGAACAA GCCTCCGTAC CCTGCTCTGG GAGGAAACAT GAAGAAGATT 
GAAAACTGTA ACTACGCGGT GGAGCTTGGG AAGAATGAGG CCAAGTTCTC TTTGGTTGGC 
ATCGCCGGGC AGGACTTAAA TGAAGGGAAC GCAACACTTA CCCTGGCACT GGTGTGGCAG 
TTGATGAGAA GATACACGCT GAAAGTGTTA TCAGACCTTG GAGAAGGTGA AAAAGTCACT 
GATGATATCA TTATTAAATG GGTCAACCAG ACCCTTAAAA GTGCAAACAA GAGCACTTCT 
ATTTCCAGCT TCAAGGATAA ATCTATTAGT ACAAGTTTGC CTGTCTTAGA TCTAATAGAT 
GCCATTGCAC CAAATGCAGT CCGTCAAGAA ATGATCAAGA GAGAGCACTT GACTGATGAG 
GACAAACTGA ACAATGCTAA ATACGCCATT TCAGTTGCTC GGAAGATCGG TGCCCGGATA 
TACGCGCTGC CTGACGACCT CGTGGAAGTG AAGCCGAAGA TGGTGATGAC TGTGTTTGCC 
TGCTTAATGG GGAAAGGATT GAACAGACTG AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510811.1
CDS	304..1959
Translation	MQELKSKDISKTFRKIINKREGITAIGGTSSISSEGTQHSYSEEEKVAFVNWINKALENDADCSHLLPMNPNDGSLFKSLADGILLCKMINLSEPDTIDERAINKKKLTPFTVSENLNLALNSASAIGCTVVNIGAQDLKEGKPHLVLGLLWQIIKVGLFADIEISRNEALIALLKDGEDLEELMKLSPEELLLRWVNYHLTNAGWRTINNFSQDIKDSKAYFHLLNQIAPKGDRDDGPAVAIDLSGFNEKNDLKRAGFMLQEADKLGCRQFVTPADVVSGNPKLNLAFVANLFNTYPCLHKPDNNDIDLNLLEGESKEERTFRNWMNSLGVNPYINHLYSDLADALVIFQLYEMIRVPVNWSQVNKPPYPALGGNMKKIENCNYAVELGKNEAKFSLVGIAGQDLNEGNATLTLALVWQLMRRYTLKVLSDLGEGEKVTDDIIIKWVNQTLKSANKSTSISSFKDKSISTSLPVLDLIDAIAPNAVRQEMIKREHLTDEDKLNNAKYAISVARKIGARIYALPDDLVEVKPKMVMTVFACLMGKGLNRLK

Target ORF information:

RefSeq Version	XM_006510748.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus plastin 1 (I-isoform) (Pls1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510748.1

ORF Insert Sequence:

ATGCAAGAGC TAAAAAGCAA AGATATCAGC AAAACGTTTC GAAAAATAAT TAACAAAAGG 
GAAGGGATCA CTGCCATCGG GGGGACGTCA TCCATCTCCA GCGAGGGCAC CCAGCATTCG 
TACTCAGAGG AAGAAAAAGT GGCTTTTGTG AACTGGATAA ACAAAGCTCT GGAGAATGAT 
GCAGACTGTA GCCACCTCCT ACCCATGAAC CCCAACGACG GCAGCCTTTT CAAGTCGCTG 
GCAGATGGCA TCCTTCTCTG CAAAATGATC AACTTATCCG AGCCAGATAC GATTGACGAG 
AGAGCCATCA ATAAGAAGAA GCTCACTCCG TTCACGGTGT CGGAAAATCT GAACCTAGCA 
CTGAACTCTG CCTCGGCCAT CGGTTGCACC GTGGTCAACA TTGGTGCTCA GGACCTCAAG 
GAAGGAAAGC CTCACTTGGT CTTGGGGCTT CTCTGGCAGA TCATCAAAGT GGGTCTTTTT 
GCTGATATCG AGATTTCCAG GAATGAAGCC TTGATCGCGT TGCTAAAAGA TGGCGAGGAC 
CTGGAGGAGC TGATGAAGCT CTCTCCTGAG GAACTGCTGC TCCGATGGGT GAACTACCAT 
CTGACCAATG CGGGCTGGCG CACCATCAAC AACTTCAGCC AGGACATCAA GGATTCAAAA 
GCCTATTTCC ACCTTCTTAA TCAGATTGCT CCTAAAGGCG ACCGAGATGA TGGGCCTGCC 
GTAGCCATTG ATCTTTCAGG ATTTAATGAG AAAAACGACC TGAAGCGTGC TGGATTTATG 
CTCCAGGAAG CGGATAAACT TGGCTGCAGA CAGTTTGTCA CTCCTGCAGA TGTGGTTTCA 
GGGAACCCTA AGCTAAACTT AGCCTTTGTA GCGAATCTTT TTAACACATA CCCATGCCTA 
CACAAGCCTG ACAATAATGA CATTGATCTC AACTTACTGG AAGGCGAGAG CAAGGAGGAG 
AGGACATTCC GCAACTGGAT GAACTCGCTG GGCGTGAACC CGTACATCAA TCACCTGTAC 
AGTGACCTTG CAGACGCTTT GGTGATCTTT CAGCTCTACG AGATGATCCG CGTGCCAGTC 
AACTGGAGCC AGGTGAACAA GCCTCCGTAC CCTGCTCTGG GAGGAAACAT GAAGAAGATT 
GAAAACTGTA ACTACGCGGT GGAGCTTGGG AAGAATGAGG CCAAGTTCTC TTTGGTTGGC 
ATCGCCGGGC AGGACTTAAA TGAAGGGAAC GCAACACTTA CCCTGGCACT GGTGTGGCAG 
TTGATGAGAA GATACACGCT GAAAGTGTTA TCAGACCTTG GAGAAGGTGA AAAAGTCACT 
GATGATATCA TTATTAAATG GGTCAACCAG ACCCTTAAAA GTGCAAACAA GAGCACTTCT 
ATTTCCAGCT TCAAGGATAA ATCTATTAGT ACAAGTTTGC CTGTCTTAGA TCTAATAGAT 
GCCATTGCAC CAAATGCAGT CCGTCAAGAA ATGATCAAGA GAGAGCACTT GACTGATGAG 
GACAAACTGA ACAATGCTAA ATACGCCATT TCAGTTGCTC GGAAGATCGG TGCCCGGATA 
TACGCGCTGC CTGACGACCT CGTGGAAGTG AAGCCGAAGA TGGTGATGAC TGTGTTTGCC 
TGCTTAATGG GGAAAGGATT GAACAGACTG AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pls1 ( XM_006510747.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510810.1 Mus musculus plastin 1 (I-isoform) (Pls1), transcript variant X2, mRNA.

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CloneID	OMu12491
Clone ID Related Accession (Same CDS sequence)	XM_006510746.3 , XM_006510747.2 , XM_006510747.3 , NM_001033210.3
Accession Version	XM_006510747.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	plastin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510747.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACA GTACCACCAC CATTTCTCGG GAGGAGCTTG AAGAGCTACA AGAAGCGTTT 
AATAAAATAG ATATTGACAA CAGTGGATAT GTTAGTGACT ATGAACTTCA GGACCTATTT 
AAGGAGGCAA GCCTTCCCCT TCCTGGGTAC AAGGTGCGCG AGATTGTGGA GAAAATATTA 
GTAGTTGCCG ACAACAACAA AGATGGTAAA ATCAGTTTTG AGGAGTTCGT GTCGCTGATG 
CAAGAGCTAA AAAGCAAAGA TATCAGCAAA ACGTTTCGAA AAATAATTAA CAAAAGGGAA 
GGGATCACTG CCATCGGGGG GACGTCATCC ATCTCCAGCG AGGGCACCCA GCATTCGTAC 
TCAGAGGAAG AAAAAGTGGC TTTTGTGAAC TGGATAAACA AAGCTCTGGA GAATGATGCA 
GACTGTAGCC ACCTCCTACC CATGAACCCC AACGACGGCA GCCTTTTCAA GTCGCTGGCA 
GATGGCATCC TTCTCTGCAA AATGATCAAC TTATCCGAGC CAGATACGAT TGACGAGAGA 
GCCATCAATA AGAAGAAGCT CACTCCGTTC ACGGTGTCGG AAAATCTGAA CCTAGCACTG 
AACTCTGCCT CGGCCATCGG TTGCACCGTG GTCAACATTG GTGCTCAGGA CCTCAAGGAA 
GGAAAGCCTC ACTTGGTCTT GGGGCTTCTC TGGCAGATCA TCAAAGTGGG TCTTTTTGCT 
GATATCGAGA TTTCCAGGAA TGAAGCCTTG ATCGCGTTGC TAAAAGATGG CGAGGACCTG 
GAGGAGCTGA TGAAGCTCTC TCCTGAGGAA CTGCTGCTCC GATGGGTGAA CTACCATCTG 
ACCAATGCGG GCTGGCGCAC CATCAACAAC TTCAGCCAGG ACATCAAGGA TTCAAAAGCC 
TATTTCCACC TTCTTAATCA GATTGCTCCT AAAGGCGACC GAGATGATGG GCCTGCCGTA 
GCCATTGATC TTTCAGGATT TAATGAGAAA AACGACCTGA AGCGTGCTGG ATTTATGCTC 
CAGGAAGCGG ATAAACTTGG CTGCAGACAG TTTGTCACTC CTGCAGATGT GGTTTCAGGG 
AACCCTAAGC TAAACTTAGC CTTTGTAGCG AATCTTTTTA ACACATACCC ATGCCTACAC 
AAGCCTGACA ATAATGACAT TGATCTCAAC TTACTGGAAG GCGAGAGCAA GGAGGAGAGG 
ACATTCCGCA ACTGGATGAA CTCGCTGGGC GTGAACCCGT ACATCAATCA CCTGTACAGT 
GACCTTGCAG ACGCTTTGGT GATCTTTCAG CTCTACGAGA TGATCCGCGT GCCAGTCAAC 
TGGAGCCAGG TGAACAAGCC TCCGTACCCT GCTCTGGGAG GAAACATGAA GAAGATTGAA 
AACTGTAACT ACGCGGTGGA GCTTGGGAAG AATGAGGCCA AGTTCTCTTT GGTTGGCATC 
GCCGGGCAGG ACTTAAATGA AGGGAACGCA ACACTTACCC TGGCACTGGT GTGGCAGTTG 
ATGAGAAGAT ACACGCTGAA AGTGTTATCA GACCTTGGAG AAGGTGAAAA AGTCACTGAT 
GATATCATTA TTAAATGGGT CAACCAGACC CTTAAAAGTG CAAACAAGAG CACTTCTATT 
TCCAGCTTCA AGGATAAATC TATTAGTACA AGTTTGCCTG TCTTAGATCT AATAGATGCC 
ATTGCACCAA ATGCAGTCCG TCAAGAAATG ATCAAGAGAG AGCACTTGAC TGATGAGGAC 
AAACTGAACA ATGCTAAATA CGCCATTTCA GTTGCTCGGA AGATCGGTGC CCGGATATAC 
GCGCTGCCTG ACGACCTCGT GGAAGTGAAG CCGAAGATGG TGATGACTGT GTTTGCCTGC 
TTAATGGGGA AAGGATTGAA CAGACTGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510810.1
CDS	59..1951
Translation	MENSTTTISREELEELQEAFNKIDIDNSGYVSDYELQDLFKEASLPLPGYKVREIVEKILVVADNNKDGKISFEEFVSLMQELKSKDISKTFRKIINKREGITAIGGTSSISSEGTQHSYSEEEKVAFVNWINKALENDADCSHLLPMNPNDGSLFKSLADGILLCKMINLSEPDTIDERAINKKKLTPFTVSENLNLALNSASAIGCTVVNIGAQDLKEGKPHLVLGLLWQIIKVGLFADIEISRNEALIALLKDGEDLEELMKLSPEELLLRWVNYHLTNAGWRTINNFSQDIKDSKAYFHLLNQIAPKGDRDDGPAVAIDLSGFNEKNDLKRAGFMLQEADKLGCRQFVTPADVVSGNPKLNLAFVANLFNTYPCLHKPDNNDIDLNLLEGESKEERTFRNWMNSLGVNPYINHLYSDLADALVIFQLYEMIRVPVNWSQVNKPPYPALGGNMKKIENCNYAVELGKNEAKFSLVGIAGQDLNEGNATLTLALVWQLMRRYTLKVLSDLGEGEKVTDDIIIKWVNQTLKSANKSTSISSFKDKSISTSLPVLDLIDAIAPNAVRQEMIKREHLTDEDKLNNAKYAISVARKIGARIYALPDDLVEVKPKMVMTVFACLMGKGLNRLK

Target ORF information:

RefSeq Version	XM_006510747.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus plastin 1 (I-isoform) (Pls1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510747.3

ORF Insert Sequence:

ATGGAAAACA GTACCACCAC CATTTCTCGG GAGGAGCTTG AAGAGCTACA AGAAGCGTTT 
AATAAAATAG ATATTGACAA CAGTGGATAT GTTAGTGACT ATGAACTTCA GGACCTATTT 
AAGGAGGCAA GCCTTCCCCT TCCTGGGTAC AAGGTGCGCG AGATTGTGGA GAAAATATTA 
GTAGTTGCCG ACAACAACAA AGATGGTAAA ATCAGTTTTG AGGAGTTCGT GTCGCTGATG 
CAAGAGCTAA AAAGCAAAGA TATCAGCAAA ACGTTTCGAA AAATAATTAA CAAAAGGGAA 
GGGATCACTG CCATCGGGGG GACGTCATCC ATCTCCAGCG AGGGCACCCA GCATTCGTAC 
TCAGAGGAAG AAAAAGTGGC TTTTGTGAAC TGGATAAACA AAGCTCTGGA GAATGATGCA 
GACTGTAGCC ACCTCCTACC CATGAACCCC AACGACGGCA GCCTTTTCAA GTCGCTGGCA 
GATGGCATCC TTCTCTGCAA AATGATCAAC TTATCCGAGC CAGATACGAT TGACGAGAGA 
GCCATCAATA AGAAGAAGCT CACTCCGTTC ACGGTGTCGG AAAATCTGAA CCTAGCACTG 
AACTCTGCCT CGGCCATCGG TTGCACCGTG GTCAACATTG GTGCTCAGGA CCTCAAGGAA 
GGAAAGCCTC ACTTGGTCTT GGGGCTTCTC TGGCAGATCA TCAAAGTGGG TCTTTTTGCT 
GATATCGAGA TTTCCAGGAA TGAAGCCTTG ATCGCGTTGC TAAAAGATGG CGAGGACCTG 
GAGGAGCTGA TGAAGCTCTC TCCTGAGGAA CTGCTGCTCC GATGGGTGAA CTACCATCTG 
ACCAATGCGG GCTGGCGCAC CATCAACAAC TTCAGCCAGG ACATCAAGGA TTCAAAAGCC 
TATTTCCACC TTCTTAATCA GATTGCTCCT AAAGGCGACC GAGATGATGG GCCTGCCGTA 
GCCATTGATC TTTCAGGATT TAATGAGAAA AACGACCTGA AGCGTGCTGG ATTTATGCTC 
CAGGAAGCGG ATAAACTTGG CTGCAGACAG TTTGTCACTC CTGCAGATGT GGTTTCAGGG 
AACCCTAAGC TAAACTTAGC CTTTGTAGCG AATCTTTTTA ACACATACCC ATGCCTACAC 
AAGCCTGACA ATAATGACAT TGATCTCAAC TTACTGGAAG GCGAGAGCAA GGAGGAGAGG 
ACATTCCGCA ACTGGATGAA CTCGCTGGGC GTGAACCCGT ACATCAATCA CCTGTACAGT 
GACCTTGCAG ACGCTTTGGT GATCTTTCAG CTCTACGAGA TGATCCGCGT GCCAGTCAAC 
TGGAGCCAGG TGAACAAGCC TCCGTACCCT GCTCTGGGAG GAAACATGAA GAAGATTGAA 
AACTGTAACT ACGCGGTGGA GCTTGGGAAG AATGAGGCCA AGTTCTCTTT GGTTGGCATC 
GCCGGGCAGG ACTTAAATGA AGGGAACGCA ACACTTACCC TGGCACTGGT GTGGCAGTTG 
ATGAGAAGAT ACACGCTGAA AGTGTTATCA GACCTTGGAG AAGGTGAAAA AGTCACTGAT 
GATATCATTA TTAAATGGGT CAACCAGACC CTTAAAAGTG CAAACAAGAG CACTTCTATT 
TCCAGCTTCA AGGATAAATC TATTAGTACA AGTTTGCCTG TCTTAGATCT AATAGATGCC 
ATTGCACCAA ATGCAGTCCG TCAAGAAATG ATCAAGAGAG AGCACTTGAC TGATGAGGAC 
AAACTGAACA ATGCTAAATA CGCCATTTCA GTTGCTCGGA AGATCGGTGC CCGGATATAC 
GCGCTGCCTG ACGACCTCGT GGAAGTGAAG CCGAAGATGG TGATGACTGT GTTTGCCTGC 
TTAATGGGGA AAGGATTGAA CAGACTGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid.
The Journal of cell biology215(4)467-482(2016 Nov)
Krey JF,Krystofiak ES,Dumont RA,Vijayakumar S,Choi D,Rivero F,Kachar B,Jones SM,Barr-Gillespie PG

L-Plastin promotes podosome longevity and supports macrophage motility.
Molecular immunology7879-88(2016 10)
Zhou JY,Szasz TP,Stewart-Hutchinson PJ,Sivapalan J,Todd EM,Deady LE,Cooper JA,Onken MD,Morley SC

Absence of plastin 1 causes abnormal maintenance of hair cell stereocilia and a moderate form of hearing loss in mice.
Human molecular genetics24(1)37-49(2015 Jan)
Taylor R,Bullen A,Johnson SL,Grimm-G?nter EM,Rivero F,Marcotti W,Forge A,Daudet N

Expression analysis of mouse Rhobtb3 using a LacZ reporter and preliminary characterization of a knockout strain.
Histochemistry and cell biology142(5)511-28(2014 Nov)
Lutz J,Grimm-G?nter EM,Joshi P,Rivero F

L-plastin is essential for alveolar macrophage production and control of pulmonary pneumococcal infection.
Infection and immunity82(5)1982-93(2014 May)
Deady LE,Todd EM,Davis CG,Zhou JY,Topcagic N,Edelson BT,Ferkol TW,Cooper MA,Muenzer JT,Morley SC

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Pls1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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