syngap1a cDNA ORF clone, Danio rerio(zebrafish)

The following syngap1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the syngap1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa48376 XM_009293918.2
Latest version!
Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa23693 XM_005159316.4
Latest version!
Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa23694 XM_003200501.5
Latest version!
Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa23695 XM_005159317.4
Latest version!
Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa23696 XM_005159318.4
Latest version!
Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa23696 XM_009293917.3
Latest version!
Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa23696 XM_021468219.1
Latest version!
Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa48376 XM_009293918.1 Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa23695 XM_005159317.3 Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa23694 XM_003200501.4 Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa23696 XM_009293917.2 Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa23693 XM_005159316.3 Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa23696 XM_005159318.3 Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa48376
    Clone ID Related Accession (Same CDS sequence) XM_009293918.1 , XM_009293918.2
    Accession Version XM_009293918.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ras/Rap GTPase-activating protein SynGAP isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATGAGT TTCTGGAGAT GGAGTGGGGC TCCATGCAGC AGTTCCTCTA CGAGATCTCC 
    AATCTGGAGT GTGTGAGTAA CGCCGGGGCG TTCGAGGGCT ACATCGACCT GGGCCGCGAG
    CTCTCCATCC TGCACAGTCT GCTGTGGGAG GTCATGGCCC AGCTCAGCAA GGATGCGATC
    CTGAAGCTGG GTCCTCTGCC GCGGCTGCTG AATGACATCA GTGTGGCCCT GCGTAACCCT
    CAGCTGCACA GACAGGCCAG CCACCAGCCA CCCGAGCGAC ACCTGAGCCG GCCTGCGTTC
    AGCAGACTCG CCACCAATGA CTTCCAGAGC CTCATGATGA GAGACCTCAA CAGCTCCATA
    GACATCTCTC GACTGCCGTC TCCTACAGCC GGTCTGTCCG GCAGCGGTGT TCTCTCTCAC
    CCGGGCATGG CTGCGTTCTC AGAGCGAGAT CCCCGCGGCA ACAAAGACGT CTTCTATGTG
    ACCCGCCCAC CTCTCGCACG CTCAAGCCCC GCCTACTGCA CCAGCAGCTC TGACATCACT
    GAGCCAGACC CCAAGGTACT CAGCGTCAAT AAGAGTGTGT CTATGATGGA CCTGCAGGAC
    TCGCGCATCA ACAGCGTGTC TAACCTGCAC TCAGTGAATG ATCTGCTGAA CTCGTCTCAG
    GGTTCTCTGG CTGGGTTCGG TCCGCTCGGC GGTGCCCCCC TCAGGGCTGG AGGACGCGTG
    TCTGCGGGCT CGGGCGGCTC CAGCATGTCT GGCGGTCTGC GTTTGACCAC AGACTCTCTG
    TCGCAGCAGC AACAGCAGGC CGCCGCCGCC ATGCACGTGC CGCTGTCCTT CCAGAACCCT
    CTGTTCCACC TAGCATCAGA TGGGCCGCAG TATCACACAC CTCCACACGG GCCCACTGCT
    GTACCGCCAC CGCTGCTGCT GGCGCCCGAG CCAGAGAACG GGCATGTGGG TTTCGGCGTT
    CCCACATTCG CCCACAGTGG GTTCTCCCGC AGTGAAGATC TGTCAGCGCT GCGGACGCAG
    AACAGTCTGG TGCAGCCCAA CATCGTGCAC TCGCACAGCT ACAGCGACGA CTTCACACGA
    CACAACCAGG CCGACTACGC CCGCAGACAG CTGTCTCTGC AGGTGCAGGA GAATCTCCAG
    CAGCAGGTGC TGATGGGAAT GACCCCGCAG ACGGCCACAG GCACCGGCAC TCCTGCTTCT
    CTGGCCACAC CTCCCACCAC CATTCATCCT GTCCGTCAGG CCTCCATGGC TCCGCCCCCT
    CCTCAACGCG CGAAGCCTCA GCCCGCCCAC CAGCTGTCCG TCAGCTCGGC TGTAAACTCC
    ACCCCCCCTA AGACCCGCCC ACAGAGCGGC AACCTCCTCC AATCGCCTGA GTCTAGTTTT
    GGAGGGAGGC AGTTGACAAG GCAGCAGCTG TCAGTCAAGG ACAGCCCCGC CCCCGGGTTG
    CCGCATCAGT CCAGCAATGC GTCGGAGAAT CCCGCTCCTC AGAGCGCAGC CAATGAGAGC
    ACAGAGAATA CGCCGACCAA CCAATCAGCA AAAAACACAC AGCAGAGCAA CCAACAACAC
    CTGCTCAAGC CATCCGGGAA CAAACAGGGC TCTAAGTCTC CCTCCACCCT GAACCCCACC
    TCTGCAGCCT CCGAGCGCAC CGTGGCCTGG GTCTCCAACA TGCCACATCT GTCTGCAGAC
    ATCGAGAGTC TGCGCGTGGA CAGAGAGGAG TTCAAACTCA AGGAGCACTC CAAGAGCATG
    GATGAGTCCC GCCTGGACCG GGTGCGTGAG TACGAGGAGG AGATCAACTC CCTGAAGGAG
    CGTCTGATGA TGTCCCACCG TAAGCTGGAG GAGTACGAGC GACGGCTACA CTCGCAGGAG
    CAGCAGACCA GCAAGATTCT GCTGCAGTAC CAGAGCCGGC TGGACGACAG CGAGAGGCGC
    CTGCGGCAGC AGCAGGTGGA GAAGGACTCG CAGATCAAGG GCATCATCAA CAGGTTGATG
    GCGGTAGAGG ATGAGCTGAG GGGAAGTGGA ATGCCTGACC TCAAAACCAG AATATTCACA
    GAACAGCCGA TTTATGGTGC CCACTCTGGA ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292193.1
    CDS115..2190
    Translation

    Target ORF information:

    RefSeq Version XM_009293918.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293918.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATGAGT TTCTGGAGAT GGAGTGGGGC TCCATGCAGC AGTTCCTCTA CGAGATCTCC 
    AATCTGGAGT GTGTGAGTAA CGCCGGGGCG TTCGAGGGCT ACATCGACCT GGGCCGCGAG
    CTCTCCATCC TGCACAGTCT GCTGTGGGAG GTCATGGCCC AGCTCAGCAA GGATGCGATC
    CTGAAGCTGG GTCCTCTGCC GCGGCTGCTG AATGACATCA GTGTGGCCCT GCGTAACCCT
    CAGCTGCACA GACAGGCCAG CCACCAGCCA CCCGAGCGAC ACCTGAGCCG GCCTGCGTTC
    AGCAGACTCG CCACCAATGA CTTCCAGAGC CTCATGATGA GAGACCTCAA CAGCTCCATA
    GACATCTCTC GACTGCCGTC TCCTACAGCC GGTCTGTCCG GCAGCGGTGT TCTCTCTCAC
    CCGGGCATGG CTGCGTTCTC AGAGCGAGAT CCCCGCGGCA ACAAAGACGT CTTCTATGTG
    ACCCGCCCAC CTCTCGCACG CTCAAGCCCC GCCTACTGCA CCAGCAGCTC TGACATCACT
    GAGCCAGACC CCAAGGTACT CAGCGTCAAT AAGAGTGTGT CTATGATGGA CCTGCAGGAC
    TCGCGCATCA ACAGCGTGTC TAACCTGCAC TCAGTGAATG ATCTGCTGAA CTCGTCTCAG
    GGTTCTCTGG CTGGGTTCGG TCCGCTCGGC GGTGCCCCCC TCAGGGCTGG AGGACGCGTG
    TCTGCGGGCT CGGGCGGCTC CAGCATGTCT GGCGGTCTGC GTTTGACCAC AGACTCTCTG
    TCGCAGCAGC AACAGCAGGC CGCCGCCGCC ATGCACGTGC CGCTGTCCTT CCAGAACCCT
    CTGTTCCACC TAGCATCAGA TGGGCCGCAG TATCACACAC CTCCACACGG GCCCACTGCT
    GTACCGCCAC CGCTGCTGCT GGCGCCCGAG CCAGAGAACG GGCATGTGGG TTTCGGCGTT
    CCCACATTCG CCCACAGTGG GTTCTCCCGC AGTGAAGATC TGTCAGCGCT GCGGACGCAG
    AACAGTCTGG TGCAGCCCAA CATCGTGCAC TCGCACAGCT ACAGCGACGA CTTCACACGA
    CACAACCAGG CCGACTACGC CCGCAGACAG CTGTCTCTGC AGGTGCAGGA GAATCTCCAG
    CAGCAGGTGC TGATGGGAAT GACCCCGCAG ACGGCCACAG GCACCGGCAC TCCTGCTTCT
    CTGGCCACAC CTCCCACCAC CATTCATCCT GTCCGTCAGG CCTCCATGGC TCCGCCCCCT
    CCTCAACGCG CGAAGCCTCA GCCCGCCCAC CAGCTGTCCG TCAGCTCGGC TGTAAACTCC
    ACCCCCCCTA AGACCCGCCC ACAGAGCGGC AACCTCCTCC AATCGCCTGA GTCTAGTTTT
    GGAGGGAGGC AGTTGACAAG GCAGCAGCTG TCAGTCAAGG ACAGCCCCGC CCCCGGGTTG
    CCGCATCAGT CCAGCAATGC GTCGGAGAAT CCCGCTCCTC AGAGCGCAGC CAATGAGAGC
    ACAGAGAATA CGCCGACCAA CCAATCAGCA AAAAACACAC AGCAGAGCAA CCAACAACAC
    CTGCTCAAGC CATCCGGGAA CAAACAGGGC TCTAAGTCTC CCTCCACCCT GAACCCCACC
    TCTGCAGCCT CCGAGCGCAC CGTGGCCTGG GTCTCCAACA TGCCACATCT GTCTGCAGAC
    ATCGAGAGTC TGCGCGTGGA CAGAGAGGAG TTCAAACTCA AGGAGCACTC CAAGAGCATG
    GATGAGTCCC GCCTGGACCG GGTGCGTGAG TACGAGGAGG AGATCAACTC CCTGAAGGAG
    CGTCTGATGA TGTCCCACCG TAAGCTGGAG GAGTACGAGC GACGGCTACA CTCGCAGGAG
    CAGCAGACCA GCAAGATTCT GCTGCAGTAC CAGAGCCGGC TGGACGACAG CGAGAGGCGC
    CTGCGGCAGC AGCAGGTGGA GAAGGACTCG CAGATCAAGG GCATCATCAA CAGGTTGATG
    GCGGTAGAGG ATGAGCTGAG GGGAAGTGGA ATGCCTGACC TCAAAACCAG AATATTCACA
    GAACAGCCGA TTTATGGTGC CCACTCTGGA ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48376
    Clone ID Related Accession (Same CDS sequence) XM_009293918.1 , XM_009293918.2
    Accession Version XM_009293918.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras/Rap GTPase-activating protein SynGAP isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATGAGT TTCTGGAGAT GGAGTGGGGC TCCATGCAGC AGTTCCTCTA CGAGATCTCC 
    AATCTGGAGT GTGTGAGTAA CGCCGGGGCG TTCGAGGGCT ACATCGACCT GGGCCGCGAG
    CTCTCCATCC TGCACAGTCT GCTGTGGGAG GTCATGGCCC AGCTCAGCAA GGATGCGATC
    CTGAAGCTGG GTCCTCTGCC GCGGCTGCTG AATGACATCA GTGTGGCCCT GCGTAACCCT
    CAGCTGCACA GACAGGCCAG CCACCAGCCA CCCGAGCGAC ACCTGAGCCG GCCTGCGTTC
    AGCAGACTCG CCACCAATGA CTTCCAGAGC CTCATGATGA GAGACCTCAA CAGCTCCATA
    GACATCTCTC GACTGCCGTC TCCTACAGCC GGTCTGTCCG GCAGCGGTGT TCTCTCTCAC
    CCGGGCATGG CTGCGTTCTC AGAGCGAGAT CCCCGCGGCA ACAAAGACGT CTTCTATGTG
    ACCCGCCCAC CTCTCGCACG CTCAAGCCCC GCCTACTGCA CCAGCAGCTC TGACATCACT
    GAGCCAGACC CCAAGGTACT CAGCGTCAAT AAGAGTGTGT CTATGATGGA CCTGCAGGAC
    TCGCGCATCA ACAGCGTGTC TAACCTGCAC TCAGTGAATG ATCTGCTGAA CTCGTCTCAG
    GGTTCTCTGG CTGGGTTCGG TCCGCTCGGC GGTGCCCCCC TCAGGGCTGG AGGACGCGTG
    TCTGCGGGCT CGGGCGGCTC CAGCATGTCT GGCGGTCTGC GTTTGACCAC AGACTCTCTG
    TCGCAGCAGC AACAGCAGGC CGCCGCCGCC ATGCACGTGC CGCTGTCCTT CCAGAACCCT
    CTGTTCCACC TAGCATCAGA TGGGCCGCAG TATCACACAC CTCCACACGG GCCCACTGCT
    GTACCGCCAC CGCTGCTGCT GGCGCCCGAG CCAGAGAACG GGCATGTGGG TTTCGGCGTT
    CCCACATTCG CCCACAGTGG GTTCTCCCGC AGTGAAGATC TGTCAGCGCT GCGGACGCAG
    AACAGTCTGG TGCAGCCCAA CATCGTGCAC TCGCACAGCT ACAGCGACGA CTTCACACGA
    CACAACCAGG CCGACTACGC CCGCAGACAG CTGTCTCTGC AGGTGCAGGA GAATCTCCAG
    CAGCAGGTGC TGATGGGAAT GACCCCGCAG ACGGCCACAG GCACCGGCAC TCCTGCTTCT
    CTGGCCACAC CTCCCACCAC CATTCATCCT GTCCGTCAGG CCTCCATGGC TCCGCCCCCT
    CCTCAACGCG CGAAGCCTCA GCCCGCCCAC CAGCTGTCCG TCAGCTCGGC TGTAAACTCC
    ACCCCCCCTA AGACCCGCCC ACAGAGCGGC AACCTCCTCC AATCGCCTGA GTCTAGTTTT
    GGAGGGAGGC AGTTGACAAG GCAGCAGCTG TCAGTCAAGG ACAGCCCCGC CCCCGGGTTG
    CCGCATCAGT CCAGCAATGC GTCGGAGAAT CCCGCTCCTC AGAGCGCAGC CAATGAGAGC
    ACAGAGAATA CGCCGACCAA CCAATCAGCA AAAAACACAC AGCAGAGCAA CCAACAACAC
    CTGCTCAAGC CATCCGGGAA CAAACAGGGC TCTAAGTCTC CCTCCACCCT GAACCCCACC
    TCTGCAGCCT CCGAGCGCAC CGTGGCCTGG GTCTCCAACA TGCCACATCT GTCTGCAGAC
    ATCGAGAGTC TGCGCGTGGA CAGAGAGGAG TTCAAACTCA AGGAGCACTC CAAGAGCATG
    GATGAGTCCC GCCTGGACCG GGTGCGTGAG TACGAGGAGG AGATCAACTC CCTGAAGGAG
    CGTCTGATGA TGTCCCACCG TAAGCTGGAG GAGTACGAGC GACGGCTACA CTCGCAGGAG
    CAGCAGACCA GCAAGATTCT GCTGCAGTAC CAGAGCCGGC TGGACGACAG CGAGAGGCGC
    CTGCGGCAGC AGCAGGTGGA GAAGGACTCG CAGATCAAGG GCATCATCAA CAGGTTGATG
    GCGGTAGAGG ATGAGCTGAG GGGAAGTGGA ATGCCTGACC TCAAAACCAG AATATTCACA
    GAACAGCCGA TTTATGGTGC CCACTCTGGA ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292193.1
    CDS115..2190
    Translation

    Target ORF information:

    RefSeq Version XM_009293918.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293918.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATGAGT TTCTGGAGAT GGAGTGGGGC TCCATGCAGC AGTTCCTCTA CGAGATCTCC 
    AATCTGGAGT GTGTGAGTAA CGCCGGGGCG TTCGAGGGCT ACATCGACCT GGGCCGCGAG
    CTCTCCATCC TGCACAGTCT GCTGTGGGAG GTCATGGCCC AGCTCAGCAA GGATGCGATC
    CTGAAGCTGG GTCCTCTGCC GCGGCTGCTG AATGACATCA GTGTGGCCCT GCGTAACCCT
    CAGCTGCACA GACAGGCCAG CCACCAGCCA CCCGAGCGAC ACCTGAGCCG GCCTGCGTTC
    AGCAGACTCG CCACCAATGA CTTCCAGAGC CTCATGATGA GAGACCTCAA CAGCTCCATA
    GACATCTCTC GACTGCCGTC TCCTACAGCC GGTCTGTCCG GCAGCGGTGT TCTCTCTCAC
    CCGGGCATGG CTGCGTTCTC AGAGCGAGAT CCCCGCGGCA ACAAAGACGT CTTCTATGTG
    ACCCGCCCAC CTCTCGCACG CTCAAGCCCC GCCTACTGCA CCAGCAGCTC TGACATCACT
    GAGCCAGACC CCAAGGTACT CAGCGTCAAT AAGAGTGTGT CTATGATGGA CCTGCAGGAC
    TCGCGCATCA ACAGCGTGTC TAACCTGCAC TCAGTGAATG ATCTGCTGAA CTCGTCTCAG
    GGTTCTCTGG CTGGGTTCGG TCCGCTCGGC GGTGCCCCCC TCAGGGCTGG AGGACGCGTG
    TCTGCGGGCT CGGGCGGCTC CAGCATGTCT GGCGGTCTGC GTTTGACCAC AGACTCTCTG
    TCGCAGCAGC AACAGCAGGC CGCCGCCGCC ATGCACGTGC CGCTGTCCTT CCAGAACCCT
    CTGTTCCACC TAGCATCAGA TGGGCCGCAG TATCACACAC CTCCACACGG GCCCACTGCT
    GTACCGCCAC CGCTGCTGCT GGCGCCCGAG CCAGAGAACG GGCATGTGGG TTTCGGCGTT
    CCCACATTCG CCCACAGTGG GTTCTCCCGC AGTGAAGATC TGTCAGCGCT GCGGACGCAG
    AACAGTCTGG TGCAGCCCAA CATCGTGCAC TCGCACAGCT ACAGCGACGA CTTCACACGA
    CACAACCAGG CCGACTACGC CCGCAGACAG CTGTCTCTGC AGGTGCAGGA GAATCTCCAG
    CAGCAGGTGC TGATGGGAAT GACCCCGCAG ACGGCCACAG GCACCGGCAC TCCTGCTTCT
    CTGGCCACAC CTCCCACCAC CATTCATCCT GTCCGTCAGG CCTCCATGGC TCCGCCCCCT
    CCTCAACGCG CGAAGCCTCA GCCCGCCCAC CAGCTGTCCG TCAGCTCGGC TGTAAACTCC
    ACCCCCCCTA AGACCCGCCC ACAGAGCGGC AACCTCCTCC AATCGCCTGA GTCTAGTTTT
    GGAGGGAGGC AGTTGACAAG GCAGCAGCTG TCAGTCAAGG ACAGCCCCGC CCCCGGGTTG
    CCGCATCAGT CCAGCAATGC GTCGGAGAAT CCCGCTCCTC AGAGCGCAGC CAATGAGAGC
    ACAGAGAATA CGCCGACCAA CCAATCAGCA AAAAACACAC AGCAGAGCAA CCAACAACAC
    CTGCTCAAGC CATCCGGGAA CAAACAGGGC TCTAAGTCTC CCTCCACCCT GAACCCCACC
    TCTGCAGCCT CCGAGCGCAC CGTGGCCTGG GTCTCCAACA TGCCACATCT GTCTGCAGAC
    ATCGAGAGTC TGCGCGTGGA CAGAGAGGAG TTCAAACTCA AGGAGCACTC CAAGAGCATG
    GATGAGTCCC GCCTGGACCG GGTGCGTGAG TACGAGGAGG AGATCAACTC CCTGAAGGAG
    CGTCTGATGA TGTCCCACCG TAAGCTGGAG GAGTACGAGC GACGGCTACA CTCGCAGGAG
    CAGCAGACCA GCAAGATTCT GCTGCAGTAC CAGAGCCGGC TGGACGACAG CGAGAGGCGC
    CTGCGGCAGC AGCAGGTGGA GAAGGACTCG CAGATCAAGG GCATCATCAA CAGGTTGATG
    GCGGTAGAGG ATGAGCTGAG GGGAAGTGGA ATGCCTGACC TCAAAACCAG AATATTCACA
    GAACAGCCGA TTTATGGTGC CCACTCTGGA ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23693
    Clone ID Related Accession (Same CDS sequence) XM_005159316.3 , XM_005159316.4
    Accession Version XM_005159316.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ras/Rap GTPase-activating protein SynGAP isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159316.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCTATG TGTCTTCACA AGATGGAAGG TGTGTGTCTC CATGTTCTCG GCCTCACACT 
    CCTCTGAATG GCCACGCACA CGACTCCCCC GGCTGGAGCC GACAGCTCCG CGTCAGAGAT
    GGAGACCAGT TCTACCTGCT GGATCAAGAG GAGGTTCATC CACTGCTGAT GAATGATCAT
    CGTCCCGTCT CTCACCGACA CAAACTGCTG CGCAGGACCG TCAGCGTACC TGCAGATAGC
    AGACCCCACC CTGAGTCAGA GCACGCTCGT GCTCGCAGGA AGAGCTTTGC CACGTCGCGG
    CAGAGAAGCA TGGAAACTCC GCCCACTGCC CCTACACAGC CATTTCGACA GCCTAGTTTT
    CTCAGCCGCC GCTTGAAAGG ATCAATTAAA CGAGCAAAGA GTCAACCCAA ACTCGACCGC
    ACGGGAAGCT TCCGCCACAT GATTCTCCCA CGATTCCGCA GTGCTGACCA GGAGAGGACG
    CGTCTGATGC AGAGCTTTAA GGAGTCTCAC TCTCATGAGT CTCTGCTGTC TCCGAGCAGC
    GCGGCCGAGG CTCTGGACCT CACGCTGGAC GAGGACGCCA TCATCAAACC CGTCCACTCC
    AGCATCCTCG GCCAGGAGTA CTGCTTCGAG GTGACCACGG CGTCAGGAAC CAAGTGTTTC
    GCCTGCCGCT CCGCTGCTGA GAGAGACAAA TGGATCGAGA ATCTGCAGCG AGCCGTCAAA
    CCAAACAAGG ACAACAGTCG TCGTGTGGAT AACGTGCTGA AGTTATGGAT CATCGAGGCT
    CGAGATCTGC CACCGAAGAA GCGCTACTAC TGCGAGCTGT GTCTGGACGA CATGCTGTAC
    GCTCGAACCA CCAGCAAACC GCGCACCGAC ACCGTCTTCT GGGGCGAACA CTTCGAGTTC
    AACAACCTGC CGGCCATCCG CAGCCTCCGA CTGCACCTCT ACAAGGAGAA CGACAAGAAG
    AGGAGGAAGG AGAAGAGCAC GTATCTGGGT CTGGTCAGCA TCCCGATCCC GAGCGTGACG
    GGTCGTCAGT TTGTGGAGCA GTGGTACCCG CTCATCCAGC CCAGTGTTCT GGCTAAAGGC
    GGCGGCGGCG GGGGGAAGAT CATCAACGCC TCGCTGCGCC TCAAGTCCCG CTTCCAGAGC
    ATGAACATCC TGCCCATGGA GCTCTACAAG GAGTTCGCCG AGTACGTCAC CAACAACTAC
    CGCACGCTCT GCGGAGTGCT GGAGCCGCTG CTCAGCGCCA AGAGCAAAGA GGAGGTGGCC
    TGCGCGCTAG TGCACATCCT GCAGAGCACT GGGAAGGCCA AGGACTTCCT GTCTGACATG
    GCCATGTGTG AGGTGGATCG TTTCATGGAC CGTGAGCATC TGATCTTCAG AGAAAACACT
    CTCGCCACTA AAGCCATCGA GGAGTTCCTG AAGCTCATCG CACACCGCTA CCTGAAGGAC
    ACCATCGGTG AGGGAGAGTT CATCCGTGCG CTGTACGAGT CTGAGGAGAA CTGTGAGGTG
    GACCCGATGC GAACGCCTCC GTCAGTGCTG GCCGATCATC AGGCTAACCT GCGCATGTGC
    TGTGAGCTAG CGCTCTGCAA GATCATCAAC TCTCACTGCG TGTTTCCCCG TGAGCTGAAG
    GAGGTGTTTG CGTCGTGGCG TGTGCGCTGT GCTGAGCGCG GCCGAGAGGA CATCGCCGAC
    CGCCTCATCA GCTCCTCGCT CTTCCTGCGC TTCCTGTGTC CGGCGATCAT GTCCCCGTCC
    CTCTTCAACC TGACGCAGGA GTATCCCTCC GAGCGCACCT CTCGAACCCT CACGCTCATC
    GCCAAGGTGG TGCAGAGCCT GGCCAGCTTT TCAAAGTTCA GTGGGAAGGA GGACTATCTG
    GGCTTCATGA ATGAGTTTCT GGAGATGGAG TGGGGCTCCA TGCAGCAGTT CCTCTACGAG
    ATCTCCAATC TGGAGTGTGT GAGTAACGCC GGGGCGTTCG AGGGCTACAT CGACCTGGGC
    CGCGAGCTCT CCATCCTGCA CAGTCTGCTG TGGGAGGTCA TGGCCCAGCT CAGCAAGGAT
    GCGATCCTGA AGCTGGGTCC TCTGCCGCGG CTGCTGAATG ACATCAGTGT GGCCCTGCGT
    AACCCTCAGC TGCACAGACA GGCCAGCCAC CAGCCACCCG AGCGACACCT GAGCCGGCCT
    GCGTTCAGCA GACTCGCCAC CAATGACTTC CAGAGCCTCA TGATGAGAGA CCTCAACAGC
    TCCATAGACA TCTCTCGACT GCCGTCTCCT ACAGCCGGTC TGTCCGGCAG CGGTGTTCTC
    TCTCACCCGG GCATGGCTGC GTTCTCAGAG CGAGATCCCC GCGGCAACAA AGACGTCTTC
    TATGTGACCC GCCCACCTCT CGCACGCTCA AGCCCCGCCT ACTGCACCAG CAGCTCTGAC
    ATCACTGAGC CAGACCCCAA GGTACTCAGC GTCAATAAGA GTGTGTCTAT GATGGACCTG
    CAGGACTCGC GCATCAACAG CGTGTCTAAC CTGCACTCAG TGAATGATCT GCTGAACTCG
    TCTCAGGGTT CTCTGGCTGG GTTCGGTCCG CTCGGCGGTG CCCCCCTCAG GGCTGGAGGA
    CGCGTGTCTG CGGGCTCGGG CGGCTCCAGC ATGTCTGGCG GTCTGCGTTT GACCACAGAC
    TCTCTGTCGC AGCAGCAACA GCAGGCCGCC GCCGCCATGC ACGTGCCGCT GTCCTTCCAG
    AACCCTCTGT TCCACCTAGC ATCAGATGGG CCGCAGTATC ACACACCTCC ACACGGGCCC
    ACTGCTGTAC CGCCACCGCT GCTGCTGGCG CCCGAGCCAG AGAACGGGCA TGTGGGTTTC
    GGCGTTCCCA CATTCGCCCA CAGTGGGTTC TCCCGCAGTG AAGATCTGTC AGCGCTGCGG
    ACGCAGAACA GTCTGGTGCA GCCCAACATC GTGCACTCGC ACAGCTACAG CGACGACTTC
    ACACGACACA ACCAGGCCGA CTACGCCCGC AGACAGCTGT CTCTGCAGGT GCAGGAGAAT
    CTCCAGCAGC AGGTGCTGAT GGGAATGACC CCGCAGACGG CCACAGGCAC CGGCACTCCT
    GCTTCTCTGG CCACACCTCC CACCACCATT CATCCTGTCC GTCAGGCCTC CATGGCTCCG
    CCCCCTCCTC AACGCGCGAA GCCTCAGCCC GCCCACCAGC TGTCCGTCAG CTCGGCTGTA
    AACTCCACCC CCCCTAAGAC CCGCCCACAG AGCGGCAACC TCCTCCAATC GCCTGAGTCT
    AGTTTTGGAG GGAGGCAGTT GACAAGGCAG CAGCTGTCAG TCAAGGACAG CCCCGCCCCC
    GGGTTGCCGC ATCAGTCCAG CAATGCGTCG GAGAATCCCG CTCCTCAGAG CGCAGCCAAT
    GAGAGCACAG AGAATACGCC GACCAACCAA TCAGCAAAAA ACACACAGCA GAGCAACCAA
    CAACACCTGC TCAAGCCATC CGGGAACAAA CAGGGCTCTA AGTCTCCCTC CACCCTGAAC
    CCCACCTCTG CAGCCTCCGA GCGCACCGTG GCCTGGGTCT CCAACATGCC ACATCTGTCT
    GCAGACATCG AGAGTCTGCG CGTGGACAGA GAGGAGTTCA AACTCAAGGA GCACTCCAAG
    AGCATGGATG AGTCCCGCCT GGACCGGGTG CGTGAGTACG AGGAGGAGAT CAACTCCCTG
    AAGGAGCGTC TGATGATGTC CCACCGTAAG CTGGAGGAGT ACGAGCGACG GCTACACTCG
    CAGGAGCAGC AGACCAGCAA GATTCTGCTG CAGTACCAGA GCCGGCTGGA CGACAGCGAG
    AGGCGCCTGC GGCAGCAGCA GGTGGAGAAG GACTCGCAGA TCAAGGGCAT CATCAACAGG
    TTGATGGCGG TAGAGGATGA GCTGAGGGGA AGTGGAATGC CTGACCTCAA AACCAGAATA
    TTCACAGAAC AGCCGATTTA TGGTGCCCAC TCTGGAACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159373.1
    CDS175..4116
    Translation

    Target ORF information:

    RefSeq Version XM_005159316.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159316.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCTATG TGTCTTCACA AGATGGAAGG TGTGTGTCTC CATGTTCTCG GCCTCACACT 
    CCTCTGAATG GCCACGCACA CGACTCCCCC GGCTGGAGCC GACAGCTCCG CGTCAGAGAT
    GGAGACCAGT TCTACCTGCT GGATCAAGAG GAGGTTCATC CACTGCTGAT GAATGATCAT
    CGTCCCGTCT CTCACCGACA CAAACTGCTG CGCAGGACCG TCAGCGTACC TGCAGATAGC
    AGACCCCACC CTGAGTCAGA GCACGCTCGT GCTCGCAGGA AGAGCTTTGC CACGTCGCGG
    CAGAGAAGCA TGGAAACTCC GCCCACTGCC CCTACACAGC CATTTCGACA GCCTAGTTTT
    CTCAGCCGCC GCTTGAAAGG ATCAATTAAA CGAGCAAAGA GTCAACCCAA ACTCGACCGC
    ACGGGAAGCT TCCGCCACAT GATTCTCCCA CGATTCCGCA GTGCTGACCA GGAGAGGACG
    CGTCTGATGC AGAGCTTTAA GGAGTCTCAC TCTCATGAGT CTCTGCTGTC TCCGAGCAGC
    GCGGCCGAGG CTCTGGACCT CACGCTGGAC GAGGACGCCA TCATCAAACC CGTCCACTCC
    AGCATCCTCG GCCAGGAGTA CTGCTTCGAG GTGACCACGG CGTCAGGAAC CAAGTGTTTC
    GCCTGCCGCT CCGCTGCTGA GAGAGACAAA TGGATCGAGA ATCTGCAGCG AGCCGTCAAA
    CCAAACAAGG ACAACAGTCG TCGTGTGGAT AACGTGCTGA AGTTATGGAT CATCGAGGCT
    CGAGATCTGC CACCGAAGAA GCGCTACTAC TGCGAGCTGT GTCTGGACGA CATGCTGTAC
    GCTCGAACCA CCAGCAAACC GCGCACCGAC ACCGTCTTCT GGGGCGAACA CTTCGAGTTC
    AACAACCTGC CGGCCATCCG CAGCCTCCGA CTGCACCTCT ACAAGGAGAA CGACAAGAAG
    AGGAGGAAGG AGAAGAGCAC GTATCTGGGT CTGGTCAGCA TCCCGATCCC GAGCGTGACG
    GGTCGTCAGT TTGTGGAGCA GTGGTACCCG CTCATCCAGC CCAGTGTTCT GGCTAAAGGC
    GGCGGCGGCG GGGGGAAGAT CATCAACGCC TCGCTGCGCC TCAAGTCCCG CTTCCAGAGC
    ATGAACATCC TGCCCATGGA GCTCTACAAG GAGTTCGCCG AGTACGTCAC CAACAACTAC
    CGCACGCTCT GCGGAGTGCT GGAGCCGCTG CTCAGCGCCA AGAGCAAAGA GGAGGTGGCC
    TGCGCGCTAG TGCACATCCT GCAGAGCACT GGGAAGGCCA AGGACTTCCT GTCTGACATG
    GCCATGTGTG AGGTGGATCG TTTCATGGAC CGTGAGCATC TGATCTTCAG AGAAAACACT
    CTCGCCACTA AAGCCATCGA GGAGTTCCTG AAGCTCATCG CACACCGCTA CCTGAAGGAC
    ACCATCGGTG AGGGAGAGTT CATCCGTGCG CTGTACGAGT CTGAGGAGAA CTGTGAGGTG
    GACCCGATGC GAACGCCTCC GTCAGTGCTG GCCGATCATC AGGCTAACCT GCGCATGTGC
    TGTGAGCTAG CGCTCTGCAA GATCATCAAC TCTCACTGCG TGTTTCCCCG TGAGCTGAAG
    GAGGTGTTTG CGTCGTGGCG TGTGCGCTGT GCTGAGCGCG GCCGAGAGGA CATCGCCGAC
    CGCCTCATCA GCTCCTCGCT CTTCCTGCGC TTCCTGTGTC CGGCGATCAT GTCCCCGTCC
    CTCTTCAACC TGACGCAGGA GTATCCCTCC GAGCGCACCT CTCGAACCCT CACGCTCATC
    GCCAAGGTGG TGCAGAGCCT GGCCAGCTTT TCAAAGTTCA GTGGGAAGGA GGACTATCTG
    GGCTTCATGA ATGAGTTTCT GGAGATGGAG TGGGGCTCCA TGCAGCAGTT CCTCTACGAG
    ATCTCCAATC TGGAGTGTGT GAGTAACGCC GGGGCGTTCG AGGGCTACAT CGACCTGGGC
    CGCGAGCTCT CCATCCTGCA CAGTCTGCTG TGGGAGGTCA TGGCCCAGCT CAGCAAGGAT
    GCGATCCTGA AGCTGGGTCC TCTGCCGCGG CTGCTGAATG ACATCAGTGT GGCCCTGCGT
    AACCCTCAGC TGCACAGACA GGCCAGCCAC CAGCCACCCG AGCGACACCT GAGCCGGCCT
    GCGTTCAGCA GACTCGCCAC CAATGACTTC CAGAGCCTCA TGATGAGAGA CCTCAACAGC
    TCCATAGACA TCTCTCGACT GCCGTCTCCT ACAGCCGGTC TGTCCGGCAG CGGTGTTCTC
    TCTCACCCGG GCATGGCTGC GTTCTCAGAG CGAGATCCCC GCGGCAACAA AGACGTCTTC
    TATGTGACCC GCCCACCTCT CGCACGCTCA AGCCCCGCCT ACTGCACCAG CAGCTCTGAC
    ATCACTGAGC CAGACCCCAA GGTACTCAGC GTCAATAAGA GTGTGTCTAT GATGGACCTG
    CAGGACTCGC GCATCAACAG CGTGTCTAAC CTGCACTCAG TGAATGATCT GCTGAACTCG
    TCTCAGGGTT CTCTGGCTGG GTTCGGTCCG CTCGGCGGTG CCCCCCTCAG GGCTGGAGGA
    CGCGTGTCTG CGGGCTCGGG CGGCTCCAGC ATGTCTGGCG GTCTGCGTTT GACCACAGAC
    TCTCTGTCGC AGCAGCAACA GCAGGCCGCC GCCGCCATGC ACGTGCCGCT GTCCTTCCAG
    AACCCTCTGT TCCACCTAGC ATCAGATGGG CCGCAGTATC ACACACCTCC ACACGGGCCC
    ACTGCTGTAC CGCCACCGCT GCTGCTGGCG CCCGAGCCAG AGAACGGGCA TGTGGGTTTC
    GGCGTTCCCA CATTCGCCCA CAGTGGGTTC TCCCGCAGTG AAGATCTGTC AGCGCTGCGG
    ACGCAGAACA GTCTGGTGCA GCCCAACATC GTGCACTCGC ACAGCTACAG CGACGACTTC
    ACACGACACA ACCAGGCCGA CTACGCCCGC AGACAGCTGT CTCTGCAGGT GCAGGAGAAT
    CTCCAGCAGC AGGTGCTGAT GGGAATGACC CCGCAGACGG CCACAGGCAC CGGCACTCCT
    GCTTCTCTGG CCACACCTCC CACCACCATT CATCCTGTCC GTCAGGCCTC CATGGCTCCG
    CCCCCTCCTC AACGCGCGAA GCCTCAGCCC GCCCACCAGC TGTCCGTCAG CTCGGCTGTA
    AACTCCACCC CCCCTAAGAC CCGCCCACAG AGCGGCAACC TCCTCCAATC GCCTGAGTCT
    AGTTTTGGAG GGAGGCAGTT GACAAGGCAG CAGCTGTCAG TCAAGGACAG CCCCGCCCCC
    GGGTTGCCGC ATCAGTCCAG CAATGCGTCG GAGAATCCCG CTCCTCAGAG CGCAGCCAAT
    GAGAGCACAG AGAATACGCC GACCAACCAA TCAGCAAAAA ACACACAGCA GAGCAACCAA
    CAACACCTGC TCAAGCCATC CGGGAACAAA CAGGGCTCTA AGTCTCCCTC CACCCTGAAC
    CCCACCTCTG CAGCCTCCGA GCGCACCGTG GCCTGGGTCT CCAACATGCC ACATCTGTCT
    GCAGACATCG AGAGTCTGCG CGTGGACAGA GAGGAGTTCA AACTCAAGGA GCACTCCAAG
    AGCATGGATG AGTCCCGCCT GGACCGGGTG CGTGAGTACG AGGAGGAGAT CAACTCCCTG
    AAGGAGCGTC TGATGATGTC CCACCGTAAG CTGGAGGAGT ACGAGCGACG GCTACACTCG
    CAGGAGCAGC AGACCAGCAA GATTCTGCTG CAGTACCAGA GCCGGCTGGA CGACAGCGAG
    AGGCGCCTGC GGCAGCAGCA GGTGGAGAAG GACTCGCAGA TCAAGGGCAT CATCAACAGG
    TTGATGGCGG TAGAGGATGA GCTGAGGGGA AGTGGAATGC CTGACCTCAA AACCAGAATA
    TTCACAGAAC AGCCGATTTA TGGTGCCCAC TCTGGAACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23693
    Clone ID Related Accession (Same CDS sequence) XM_005159316.3 , XM_005159316.4
    Accession Version XM_005159316.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras/Rap GTPase-activating protein SynGAP isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159316.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCTATG TGTCTTCACA AGATGGAAGG TGTGTGTCTC CATGTTCTCG GCCTCACACT 
    CCTCTGAATG GCCACGCACA CGACTCCCCC GGCTGGAGCC GACAGCTCCG CGTCAGAGAT
    GGAGACCAGT TCTACCTGCT GGATCAAGAG GAGGTTCATC CACTGCTGAT GAATGATCAT
    CGTCCCGTCT CTCACCGACA CAAACTGCTG CGCAGGACCG TCAGCGTACC TGCAGATAGC
    AGACCCCACC CTGAGTCAGA GCACGCTCGT GCTCGCAGGA AGAGCTTTGC CACGTCGCGG
    CAGAGAAGCA TGGAAACTCC GCCCACTGCC CCTACACAGC CATTTCGACA GCCTAGTTTT
    CTCAGCCGCC GCTTGAAAGG ATCAATTAAA CGAGCAAAGA GTCAACCCAA ACTCGACCGC
    ACGGGAAGCT TCCGCCACAT GATTCTCCCA CGATTCCGCA GTGCTGACCA GGAGAGGACG
    CGTCTGATGC AGAGCTTTAA GGAGTCTCAC TCTCATGAGT CTCTGCTGTC TCCGAGCAGC
    GCGGCCGAGG CTCTGGACCT CACGCTGGAC GAGGACGCCA TCATCAAACC CGTCCACTCC
    AGCATCCTCG GCCAGGAGTA CTGCTTCGAG GTGACCACGG CGTCAGGAAC CAAGTGTTTC
    GCCTGCCGCT CCGCTGCTGA GAGAGACAAA TGGATCGAGA ATCTGCAGCG AGCCGTCAAA
    CCAAACAAGG ACAACAGTCG TCGTGTGGAT AACGTGCTGA AGTTATGGAT CATCGAGGCT
    CGAGATCTGC CACCGAAGAA GCGCTACTAC TGCGAGCTGT GTCTGGACGA CATGCTGTAC
    GCTCGAACCA CCAGCAAACC GCGCACCGAC ACCGTCTTCT GGGGCGAACA CTTCGAGTTC
    AACAACCTGC CGGCCATCCG CAGCCTCCGA CTGCACCTCT ACAAGGAGAA CGACAAGAAG
    AGGAGGAAGG AGAAGAGCAC GTATCTGGGT CTGGTCAGCA TCCCGATCCC GAGCGTGACG
    GGTCGTCAGT TTGTGGAGCA GTGGTACCCG CTCATCCAGC CCAGTGTTCT GGCTAAAGGC
    GGCGGCGGCG GGGGGAAGAT CATCAACGCC TCGCTGCGCC TCAAGTCCCG CTTCCAGAGC
    ATGAACATCC TGCCCATGGA GCTCTACAAG GAGTTCGCCG AGTACGTCAC CAACAACTAC
    CGCACGCTCT GCGGAGTGCT GGAGCCGCTG CTCAGCGCCA AGAGCAAAGA GGAGGTGGCC
    TGCGCGCTAG TGCACATCCT GCAGAGCACT GGGAAGGCCA AGGACTTCCT GTCTGACATG
    GCCATGTGTG AGGTGGATCG TTTCATGGAC CGTGAGCATC TGATCTTCAG AGAAAACACT
    CTCGCCACTA AAGCCATCGA GGAGTTCCTG AAGCTCATCG CACACCGCTA CCTGAAGGAC
    ACCATCGGTG AGGGAGAGTT CATCCGTGCG CTGTACGAGT CTGAGGAGAA CTGTGAGGTG
    GACCCGATGC GAACGCCTCC GTCAGTGCTG GCCGATCATC AGGCTAACCT GCGCATGTGC
    TGTGAGCTAG CGCTCTGCAA GATCATCAAC TCTCACTGCG TGTTTCCCCG TGAGCTGAAG
    GAGGTGTTTG CGTCGTGGCG TGTGCGCTGT GCTGAGCGCG GCCGAGAGGA CATCGCCGAC
    CGCCTCATCA GCTCCTCGCT CTTCCTGCGC TTCCTGTGTC CGGCGATCAT GTCCCCGTCC
    CTCTTCAACC TGACGCAGGA GTATCCCTCC GAGCGCACCT CTCGAACCCT CACGCTCATC
    GCCAAGGTGG TGCAGAGCCT GGCCAGCTTT TCAAAGTTCA GTGGGAAGGA GGACTATCTG
    GGCTTCATGA ATGAGTTTCT GGAGATGGAG TGGGGCTCCA TGCAGCAGTT CCTCTACGAG
    ATCTCCAATC TGGAGTGTGT GAGTAACGCC GGGGCGTTCG AGGGCTACAT CGACCTGGGC
    CGCGAGCTCT CCATCCTGCA CAGTCTGCTG TGGGAGGTCA TGGCCCAGCT CAGCAAGGAT
    GCGATCCTGA AGCTGGGTCC TCTGCCGCGG CTGCTGAATG ACATCAGTGT GGCCCTGCGT
    AACCCTCAGC TGCACAGACA GGCCAGCCAC CAGCCACCCG AGCGACACCT GAGCCGGCCT
    GCGTTCAGCA GACTCGCCAC CAATGACTTC CAGAGCCTCA TGATGAGAGA CCTCAACAGC
    TCCATAGACA TCTCTCGACT GCCGTCTCCT ACAGCCGGTC TGTCCGGCAG CGGTGTTCTC
    TCTCACCCGG GCATGGCTGC GTTCTCAGAG CGAGATCCCC GCGGCAACAA AGACGTCTTC
    TATGTGACCC GCCCACCTCT CGCACGCTCA AGCCCCGCCT ACTGCACCAG CAGCTCTGAC
    ATCACTGAGC CAGACCCCAA GGTACTCAGC GTCAATAAGA GTGTGTCTAT GATGGACCTG
    CAGGACTCGC GCATCAACAG CGTGTCTAAC CTGCACTCAG TGAATGATCT GCTGAACTCG
    TCTCAGGGTT CTCTGGCTGG GTTCGGTCCG CTCGGCGGTG CCCCCCTCAG GGCTGGAGGA
    CGCGTGTCTG CGGGCTCGGG CGGCTCCAGC ATGTCTGGCG GTCTGCGTTT GACCACAGAC
    TCTCTGTCGC AGCAGCAACA GCAGGCCGCC GCCGCCATGC ACGTGCCGCT GTCCTTCCAG
    AACCCTCTGT TCCACCTAGC ATCAGATGGG CCGCAGTATC ACACACCTCC ACACGGGCCC
    ACTGCTGTAC CGCCACCGCT GCTGCTGGCG CCCGAGCCAG AGAACGGGCA TGTGGGTTTC
    GGCGTTCCCA CATTCGCCCA CAGTGGGTTC TCCCGCAGTG AAGATCTGTC AGCGCTGCGG
    ACGCAGAACA GTCTGGTGCA GCCCAACATC GTGCACTCGC ACAGCTACAG CGACGACTTC
    ACACGACACA ACCAGGCCGA CTACGCCCGC AGACAGCTGT CTCTGCAGGT GCAGGAGAAT
    CTCCAGCAGC AGGTGCTGAT GGGAATGACC CCGCAGACGG CCACAGGCAC CGGCACTCCT
    GCTTCTCTGG CCACACCTCC CACCACCATT CATCCTGTCC GTCAGGCCTC CATGGCTCCG
    CCCCCTCCTC AACGCGCGAA GCCTCAGCCC GCCCACCAGC TGTCCGTCAG CTCGGCTGTA
    AACTCCACCC CCCCTAAGAC CCGCCCACAG AGCGGCAACC TCCTCCAATC GCCTGAGTCT
    AGTTTTGGAG GGAGGCAGTT GACAAGGCAG CAGCTGTCAG TCAAGGACAG CCCCGCCCCC
    GGGTTGCCGC ATCAGTCCAG CAATGCGTCG GAGAATCCCG CTCCTCAGAG CGCAGCCAAT
    GAGAGCACAG AGAATACGCC GACCAACCAA TCAGCAAAAA ACACACAGCA GAGCAACCAA
    CAACACCTGC TCAAGCCATC CGGGAACAAA CAGGGCTCTA AGTCTCCCTC CACCCTGAAC
    CCCACCTCTG CAGCCTCCGA GCGCACCGTG GCCTGGGTCT CCAACATGCC ACATCTGTCT
    GCAGACATCG AGAGTCTGCG CGTGGACAGA GAGGAGTTCA AACTCAAGGA GCACTCCAAG
    AGCATGGATG AGTCCCGCCT GGACCGGGTG CGTGAGTACG AGGAGGAGAT CAACTCCCTG
    AAGGAGCGTC TGATGATGTC CCACCGTAAG CTGGAGGAGT ACGAGCGACG GCTACACTCG
    CAGGAGCAGC AGACCAGCAA GATTCTGCTG CAGTACCAGA GCCGGCTGGA CGACAGCGAG
    AGGCGCCTGC GGCAGCAGCA GGTGGAGAAG GACTCGCAGA TCAAGGGCAT CATCAACAGG
    TTGATGGCGG TAGAGGATGA GCTGAGGGGA AGTGGAATGC CTGACCTCAA AACCAGAATA
    TTCACAGAAC AGCCGATTTA TGGTGCCCAC TCTGGAACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159373.1
    CDS175..4116
    Translation

    Target ORF information:

    RefSeq Version XM_005159316.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159316.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCTATG TGTCTTCACA AGATGGAAGG TGTGTGTCTC CATGTTCTCG GCCTCACACT 
    CCTCTGAATG GCCACGCACA CGACTCCCCC GGCTGGAGCC GACAGCTCCG CGTCAGAGAT
    GGAGACCAGT TCTACCTGCT GGATCAAGAG GAGGTTCATC CACTGCTGAT GAATGATCAT
    CGTCCCGTCT CTCACCGACA CAAACTGCTG CGCAGGACCG TCAGCGTACC TGCAGATAGC
    AGACCCCACC CTGAGTCAGA GCACGCTCGT GCTCGCAGGA AGAGCTTTGC CACGTCGCGG
    CAGAGAAGCA TGGAAACTCC GCCCACTGCC CCTACACAGC CATTTCGACA GCCTAGTTTT
    CTCAGCCGCC GCTTGAAAGG ATCAATTAAA CGAGCAAAGA GTCAACCCAA ACTCGACCGC
    ACGGGAAGCT TCCGCCACAT GATTCTCCCA CGATTCCGCA GTGCTGACCA GGAGAGGACG
    CGTCTGATGC AGAGCTTTAA GGAGTCTCAC TCTCATGAGT CTCTGCTGTC TCCGAGCAGC
    GCGGCCGAGG CTCTGGACCT CACGCTGGAC GAGGACGCCA TCATCAAACC CGTCCACTCC
    AGCATCCTCG GCCAGGAGTA CTGCTTCGAG GTGACCACGG CGTCAGGAAC CAAGTGTTTC
    GCCTGCCGCT CCGCTGCTGA GAGAGACAAA TGGATCGAGA ATCTGCAGCG AGCCGTCAAA
    CCAAACAAGG ACAACAGTCG TCGTGTGGAT AACGTGCTGA AGTTATGGAT CATCGAGGCT
    CGAGATCTGC CACCGAAGAA GCGCTACTAC TGCGAGCTGT GTCTGGACGA CATGCTGTAC
    GCTCGAACCA CCAGCAAACC GCGCACCGAC ACCGTCTTCT GGGGCGAACA CTTCGAGTTC
    AACAACCTGC CGGCCATCCG CAGCCTCCGA CTGCACCTCT ACAAGGAGAA CGACAAGAAG
    AGGAGGAAGG AGAAGAGCAC GTATCTGGGT CTGGTCAGCA TCCCGATCCC GAGCGTGACG
    GGTCGTCAGT TTGTGGAGCA GTGGTACCCG CTCATCCAGC CCAGTGTTCT GGCTAAAGGC
    GGCGGCGGCG GGGGGAAGAT CATCAACGCC TCGCTGCGCC TCAAGTCCCG CTTCCAGAGC
    ATGAACATCC TGCCCATGGA GCTCTACAAG GAGTTCGCCG AGTACGTCAC CAACAACTAC
    CGCACGCTCT GCGGAGTGCT GGAGCCGCTG CTCAGCGCCA AGAGCAAAGA GGAGGTGGCC
    TGCGCGCTAG TGCACATCCT GCAGAGCACT GGGAAGGCCA AGGACTTCCT GTCTGACATG
    GCCATGTGTG AGGTGGATCG TTTCATGGAC CGTGAGCATC TGATCTTCAG AGAAAACACT
    CTCGCCACTA AAGCCATCGA GGAGTTCCTG AAGCTCATCG CACACCGCTA CCTGAAGGAC
    ACCATCGGTG AGGGAGAGTT CATCCGTGCG CTGTACGAGT CTGAGGAGAA CTGTGAGGTG
    GACCCGATGC GAACGCCTCC GTCAGTGCTG GCCGATCATC AGGCTAACCT GCGCATGTGC
    TGTGAGCTAG CGCTCTGCAA GATCATCAAC TCTCACTGCG TGTTTCCCCG TGAGCTGAAG
    GAGGTGTTTG CGTCGTGGCG TGTGCGCTGT GCTGAGCGCG GCCGAGAGGA CATCGCCGAC
    CGCCTCATCA GCTCCTCGCT CTTCCTGCGC TTCCTGTGTC CGGCGATCAT GTCCCCGTCC
    CTCTTCAACC TGACGCAGGA GTATCCCTCC GAGCGCACCT CTCGAACCCT CACGCTCATC
    GCCAAGGTGG TGCAGAGCCT GGCCAGCTTT TCAAAGTTCA GTGGGAAGGA GGACTATCTG
    GGCTTCATGA ATGAGTTTCT GGAGATGGAG TGGGGCTCCA TGCAGCAGTT CCTCTACGAG
    ATCTCCAATC TGGAGTGTGT GAGTAACGCC GGGGCGTTCG AGGGCTACAT CGACCTGGGC
    CGCGAGCTCT CCATCCTGCA CAGTCTGCTG TGGGAGGTCA TGGCCCAGCT CAGCAAGGAT
    GCGATCCTGA AGCTGGGTCC TCTGCCGCGG CTGCTGAATG ACATCAGTGT GGCCCTGCGT
    AACCCTCAGC TGCACAGACA GGCCAGCCAC CAGCCACCCG AGCGACACCT GAGCCGGCCT
    GCGTTCAGCA GACTCGCCAC CAATGACTTC CAGAGCCTCA TGATGAGAGA CCTCAACAGC
    TCCATAGACA TCTCTCGACT GCCGTCTCCT ACAGCCGGTC TGTCCGGCAG CGGTGTTCTC
    TCTCACCCGG GCATGGCTGC GTTCTCAGAG CGAGATCCCC GCGGCAACAA AGACGTCTTC
    TATGTGACCC GCCCACCTCT CGCACGCTCA AGCCCCGCCT ACTGCACCAG CAGCTCTGAC
    ATCACTGAGC CAGACCCCAA GGTACTCAGC GTCAATAAGA GTGTGTCTAT GATGGACCTG
    CAGGACTCGC GCATCAACAG CGTGTCTAAC CTGCACTCAG TGAATGATCT GCTGAACTCG
    TCTCAGGGTT CTCTGGCTGG GTTCGGTCCG CTCGGCGGTG CCCCCCTCAG GGCTGGAGGA
    CGCGTGTCTG CGGGCTCGGG CGGCTCCAGC ATGTCTGGCG GTCTGCGTTT GACCACAGAC
    TCTCTGTCGC AGCAGCAACA GCAGGCCGCC GCCGCCATGC ACGTGCCGCT GTCCTTCCAG
    AACCCTCTGT TCCACCTAGC ATCAGATGGG CCGCAGTATC ACACACCTCC ACACGGGCCC
    ACTGCTGTAC CGCCACCGCT GCTGCTGGCG CCCGAGCCAG AGAACGGGCA TGTGGGTTTC
    GGCGTTCCCA CATTCGCCCA CAGTGGGTTC TCCCGCAGTG AAGATCTGTC AGCGCTGCGG
    ACGCAGAACA GTCTGGTGCA GCCCAACATC GTGCACTCGC ACAGCTACAG CGACGACTTC
    ACACGACACA ACCAGGCCGA CTACGCCCGC AGACAGCTGT CTCTGCAGGT GCAGGAGAAT
    CTCCAGCAGC AGGTGCTGAT GGGAATGACC CCGCAGACGG CCACAGGCAC CGGCACTCCT
    GCTTCTCTGG CCACACCTCC CACCACCATT CATCCTGTCC GTCAGGCCTC CATGGCTCCG
    CCCCCTCCTC AACGCGCGAA GCCTCAGCCC GCCCACCAGC TGTCCGTCAG CTCGGCTGTA
    AACTCCACCC CCCCTAAGAC CCGCCCACAG AGCGGCAACC TCCTCCAATC GCCTGAGTCT
    AGTTTTGGAG GGAGGCAGTT GACAAGGCAG CAGCTGTCAG TCAAGGACAG CCCCGCCCCC
    GGGTTGCCGC ATCAGTCCAG CAATGCGTCG GAGAATCCCG CTCCTCAGAG CGCAGCCAAT
    GAGAGCACAG AGAATACGCC GACCAACCAA TCAGCAAAAA ACACACAGCA GAGCAACCAA
    CAACACCTGC TCAAGCCATC CGGGAACAAA CAGGGCTCTA AGTCTCCCTC CACCCTGAAC
    CCCACCTCTG CAGCCTCCGA GCGCACCGTG GCCTGGGTCT CCAACATGCC ACATCTGTCT
    GCAGACATCG AGAGTCTGCG CGTGGACAGA GAGGAGTTCA AACTCAAGGA GCACTCCAAG
    AGCATGGATG AGTCCCGCCT GGACCGGGTG CGTGAGTACG AGGAGGAGAT CAACTCCCTG
    AAGGAGCGTC TGATGATGTC CCACCGTAAG CTGGAGGAGT ACGAGCGACG GCTACACTCG
    CAGGAGCAGC AGACCAGCAA GATTCTGCTG CAGTACCAGA GCCGGCTGGA CGACAGCGAG
    AGGCGCCTGC GGCAGCAGCA GGTGGAGAAG GACTCGCAGA TCAAGGGCAT CATCAACAGG
    TTGATGGCGG TAGAGGATGA GCTGAGGGGA AGTGGAATGC CTGACCTCAA AACCAGAATA
    TTCACAGAAC AGCCGATTTA TGGTGCCCAC TCTGGAACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23694
    Clone ID Related Accession (Same CDS sequence) XM_003200501.4 , XM_003200501.5
    Accession Version XM_003200501.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ras/Rap GTPase-activating protein SynGAP isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003200501.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCTATG TGTCTTCACA AGATGGAAGG TGTGTGTCTC CATGTTCTCG GCCTCACACT 
    CCTCTGAATG GCCACGCACA CGACTCCCCC GGCTGGAGCC GACAGCTCCG CGTCAGAGAT
    GGAGACCAGT TCTACCTGCT GGATCAAGAG GAGGTTCATC CACTGCTGAT GAATGATCAT
    CGTCCCGTCT CTCACCGACA CAAACTGCTG CGCAGGACCG TCAGCGTACC TGCAGATAGC
    AGACCCCACC CTGAGTCAGA GCACGCTCGT GCTCGCAGGA AGAGCTTTGC CACGTCGCGG
    CAGAGAAGCA TGGAAACTCC GCCCACTGCC CCTACACAGC CATTTCGACA GCCTAGTTTT
    CTCAGCCGCC GCTTGAAAGG ATCAATTAAA CGAGCAAAGA GTCAACCCAA ACTCGACCGC
    ACGGGAAGCT TCCGCCACAT GATTCTCCCA CGATTCCGCA GTGCTGACCA GGAGAGGACG
    CGTCTGATGC AGAGCTTTAA GGAGTCTCAC TCTCATGAGT CTCTGCTGTC TCCGAGCAGC
    GCGGCCGAGG CTCTGGACCT CACGCTGGAC GAGGACGCCA TCATCAAACC CGTCCACTCC
    AGCATCCTCG GCCAGGAGTA CTGCTTCGAG GTGACCACGG CGTCAGGAAC CAAGTGTTTC
    GCCTGCCGCT CCGCTGCTGA GAGAGACAAA TGGATCGAGA ATCTGCAGCG AGCCGTCAAA
    CCAAACAAGG ACAACAGTCG TCGTGTGGAT AACGTGCTGA AGTTATGGAT CATCGAGGCT
    CGAGATCTGC CACCGAAGAA GCGCTACTAC TGCGAGCTGT GTCTGGACGA CATGCTGTAC
    GCTCGAACCA CCAGCAAACC GCGCACCGAC ACCGTCTTCT GGGGCGAACA CTTCGAGTTC
    AACAACCTGC CGGCCATCCG CAGCCTCCGA CTGCACCTCT ACAAGGAGAA CGACAAGAAG
    AGGAGGAAGG AGAAGAGCAC GTATCTGGGT CTGGTCAGCA TCCCGATCCC GAGCGTGACG
    GGTCGTCAGT TTGTGGAGCA GTGGTACCCG CTCATCCAGC CCAGTGTTCT GGCTAAAGGC
    GGCGGCGGCG GGGGGAAGAT CATCAACGCC TCGCTGCGCC TCAAGTCCCG CTTCCAGAGC
    ATGAACATCC TGCCCATGGA GCTCTACAAG GAGTTCGCCG AGTACGTCAC CAACAACTAC
    CGCACGCTCT GCGGAGTGCT GGAGCCGCTG CTCAGCGCCA AGAGCAAAGA GGAGGTGGCC
    TGCGCGCTAG TGCACATCCT GCAGAGCACT GGGAAGGCCA AGGACTTCCT GTCTGACATG
    GCCATGTGTG AGGTGGATCG TTTCATGGAC CGTGAGCATC TGATCTTCAG AGAAAACACT
    CTCGCCACTA AAGCCATCGA GGAGTTCCTG AAGCTCATCG CACACCGCTA CCTGAAGGAC
    ACCATCGGAG AGTTCATCCG TGCGCTGTAC GAGTCTGAGG AGAACTGTGA GGTGGACCCG
    ATGCGAACGC CTCCGTCAGT GCTGGCCGAT CATCAGGCTA ACCTGCGCAT GTGCTGTGAG
    CTAGCGCTCT GCAAGATCAT CAACTCTCAC TGCGTGTTTC CCCGTGAGCT GAAGGAGGTG
    TTTGCGTCGT GGCGTGTGCG CTGTGCTGAG CGCGGCCGAG AGGACATCGC CGACCGCCTC
    ATCAGCTCCT CGCTCTTCCT GCGCTTCCTG TGTCCGGCGA TCATGTCCCC GTCCCTCTTC
    AACCTGACGC AGGAGTATCC CTCCGAGCGC ACCTCTCGAA CCCTCACGCT CATCGCCAAG
    GTGGTGCAGA GCCTGGCCAG CTTTTCAAAG TTCAGTGGGA AGGAGGACTA TCTGGGCTTC
    ATGAATGAGT TTCTGGAGAT GGAGTGGGGC TCCATGCAGC AGTTCCTCTA CGAGATCTCC
    AATCTGGAGT GTGTGAGTAA CGCCGGGGCG TTCGAGGGCT ACATCGACCT GGGCCGCGAG
    CTCTCCATCC TGCACAGTCT GCTGTGGGAG GTCATGGCCC AGCTCAGCAA GGATGCGATC
    CTGAAGCTGG GTCCTCTGCC GCGGCTGCTG AATGACATCA GTGTGGCCCT GCGTAACCCT
    CAGCTGCACA GACAGGCCAG CCACCAGCCA CCCGAGCGAC ACCTGAGCCG GCCTGCGTTC
    AGCAGACTCG CCACCAATGA CTTCCAGAGC CTCATGATGA GAGACCTCAA CAGCTCCATA
    GACATCTCTC GACTGCCGTC TCCTACAGCC GGTCTGTCCG GCAGCGGTGT TCTCTCTCAC
    CCGGGCATGG CTGCGTTCTC AGAGCGAGAT CCCCGCGGCA ACAAAGACGT CTTCTATGTG
    ACCCGCCCAC CTCTCGCACG CTCAAGCCCC GCCTACTGCA CCAGCAGCTC TGACATCACT
    GAGCCAGACC CCAAGGTACT CAGCGTCAAT AAGAGTGTGT CTATGATGGA CCTGCAGGAC
    TCGCGCATCA ACAGCGTGTC TAACCTGCAC TCAGTGAATG ATCTGCTGAA CTCGTCTCAG
    GGTTCTCTGG CTGGGTTCGG TCCGCTCGGC GGTGCCCCCC TCAGGGCTGG AGGACGCGTG
    TCTGCGGGCT CGGGCGGCTC CAGCATGTCT GGCGGTCTGC GTTTGACCAC AGACTCTCTG
    TCGCAGCAGC AACAGCAGGC CGCCGCCGCC ATGCACGTGC CGCTGTCCTT CCAGAACCCT
    CTGTTCCACC TAGCATCAGA TGGGCCGCAG TATCACACAC CTCCACACGG GCCCACTGCT
    GTACCGCCAC CGCTGCTGCT GGCGCCCGAG CCAGAGAACG GGCATGTGGG TTTCGGCGTT
    CCCACATTCG CCCACAGTGG GTTCTCCCGC AGTGAAGATC TGTCAGCGCT GCGGACGCAG
    AACAGTCTGG TGCAGCCCAA CATCGTGCAC TCGCACAGCT ACAGCGACGA CTTCACACGA
    CACAACCAGG CCGACTACGC CCGCAGACAG CTGTCTCTGC AGGTGCAGGA GAATCTCCAG
    CAGCAGGTGC TGATGGGAAT GACCCCGCAG ACGGCCACAG GCACCGGCAC TCCTGCTTCT
    CTGGCCACAC CTCCCACCAC CATTCATCCT GTCCGTCAGG CCTCCATGGC TCCGCCCCCT
    CCTCAACGCG CGAAGCCTCA GCCCGCCCAC CAGCTGTCCG TCAGCTCGGC TGTAAACTCC
    ACCCCCCCTA AGACCCGCCC ACAGAGCGGC AACCTCCTCC AATCGCCTGA GTCTAGTTTT
    GGAGGGAGGC AGTTGACAAG GCAGCAGCTG TCAGTCAAGG ACAGCCCCGC CCCCGGGTTG
    CCGCATCAGT CCAGCAATGC GTCGGAGAAT CCCGCTCCTC AGAGCGCAGC CAATGAGAGC
    ACAGAGAATA CGCCGACCAA CCAATCAGCA AAAAACACAC AGCAGAGCAA CCAACAACAC
    CTGCTCAAGC CATCCGGGAA CAAACAGGGC TCTAAGTCTC CCTCCACCCT GAACCCCACC
    TCTGCAGCCT CCGAGCGCAC CGTGGCCTGG GTCTCCAACA TGCCACATCT GTCTGCAGAC
    ATCGAGAGTC TGCGCGTGGA CAGAGAGGAG TTCAAACTCA AGGAGCACTC CAAGAGCATG
    GATGAGTCCC GCCTGGACCG GGTGCGTGAG TACGAGGAGG AGATCAACTC CCTGAAGGAG
    CGTCTGATGA TGTCCCACCG TAAGCTGGAG GAGTACGAGC GACGGCTACA CTCGCAGGAG
    CAGCAGACCA GCAAGATTCT GCTGCAGTAC CAGAGCCGGC TGGACGACAG CGAGAGGCGC
    CTGCGGCAGC AGCAGGTGGA GAAGGACTCG CAGATCAAGG GCATCATCAA CAGGTTGATG
    GCGGTAGAGG ATGAGCTGAG GGGAAGTGGA ATGCCTGACC TCAAAACCAG AATATTCACA
    GAACAGCCGA TTTATGGTGC CCACTCTGGA ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003200549.2
    CDS175..4110
    Translation

    Target ORF information:

    RefSeq Version XM_003200501.4
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003200501.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCTATG TGTCTTCACA AGATGGAAGG TGTGTGTCTC CATGTTCTCG GCCTCACACT 
    CCTCTGAATG GCCACGCACA CGACTCCCCC GGCTGGAGCC GACAGCTCCG CGTCAGAGAT
    GGAGACCAGT TCTACCTGCT GGATCAAGAG GAGGTTCATC CACTGCTGAT GAATGATCAT
    CGTCCCGTCT CTCACCGACA CAAACTGCTG CGCAGGACCG TCAGCGTACC TGCAGATAGC
    AGACCCCACC CTGAGTCAGA GCACGCTCGT GCTCGCAGGA AGAGCTTTGC CACGTCGCGG
    CAGAGAAGCA TGGAAACTCC GCCCACTGCC CCTACACAGC CATTTCGACA GCCTAGTTTT
    CTCAGCCGCC GCTTGAAAGG ATCAATTAAA CGAGCAAAGA GTCAACCCAA ACTCGACCGC
    ACGGGAAGCT TCCGCCACAT GATTCTCCCA CGATTCCGCA GTGCTGACCA GGAGAGGACG
    CGTCTGATGC AGAGCTTTAA GGAGTCTCAC TCTCATGAGT CTCTGCTGTC TCCGAGCAGC
    GCGGCCGAGG CTCTGGACCT CACGCTGGAC GAGGACGCCA TCATCAAACC CGTCCACTCC
    AGCATCCTCG GCCAGGAGTA CTGCTTCGAG GTGACCACGG CGTCAGGAAC CAAGTGTTTC
    GCCTGCCGCT CCGCTGCTGA GAGAGACAAA TGGATCGAGA ATCTGCAGCG AGCCGTCAAA
    CCAAACAAGG ACAACAGTCG TCGTGTGGAT AACGTGCTGA AGTTATGGAT CATCGAGGCT
    CGAGATCTGC CACCGAAGAA GCGCTACTAC TGCGAGCTGT GTCTGGACGA CATGCTGTAC
    GCTCGAACCA CCAGCAAACC GCGCACCGAC ACCGTCTTCT GGGGCGAACA CTTCGAGTTC
    AACAACCTGC CGGCCATCCG CAGCCTCCGA CTGCACCTCT ACAAGGAGAA CGACAAGAAG
    AGGAGGAAGG AGAAGAGCAC GTATCTGGGT CTGGTCAGCA TCCCGATCCC GAGCGTGACG
    GGTCGTCAGT TTGTGGAGCA GTGGTACCCG CTCATCCAGC CCAGTGTTCT GGCTAAAGGC
    GGCGGCGGCG GGGGGAAGAT CATCAACGCC TCGCTGCGCC TCAAGTCCCG CTTCCAGAGC
    ATGAACATCC TGCCCATGGA GCTCTACAAG GAGTTCGCCG AGTACGTCAC CAACAACTAC
    CGCACGCTCT GCGGAGTGCT GGAGCCGCTG CTCAGCGCCA AGAGCAAAGA GGAGGTGGCC
    TGCGCGCTAG TGCACATCCT GCAGAGCACT GGGAAGGCCA AGGACTTCCT GTCTGACATG
    GCCATGTGTG AGGTGGATCG TTTCATGGAC CGTGAGCATC TGATCTTCAG AGAAAACACT
    CTCGCCACTA AAGCCATCGA GGAGTTCCTG AAGCTCATCG CACACCGCTA CCTGAAGGAC
    ACCATCGGAG AGTTCATCCG TGCGCTGTAC GAGTCTGAGG AGAACTGTGA GGTGGACCCG
    ATGCGAACGC CTCCGTCAGT GCTGGCCGAT CATCAGGCTA ACCTGCGCAT GTGCTGTGAG
    CTAGCGCTCT GCAAGATCAT CAACTCTCAC TGCGTGTTTC CCCGTGAGCT GAAGGAGGTG
    TTTGCGTCGT GGCGTGTGCG CTGTGCTGAG CGCGGCCGAG AGGACATCGC CGACCGCCTC
    ATCAGCTCCT CGCTCTTCCT GCGCTTCCTG TGTCCGGCGA TCATGTCCCC GTCCCTCTTC
    AACCTGACGC AGGAGTATCC CTCCGAGCGC ACCTCTCGAA CCCTCACGCT CATCGCCAAG
    GTGGTGCAGA GCCTGGCCAG CTTTTCAAAG TTCAGTGGGA AGGAGGACTA TCTGGGCTTC
    ATGAATGAGT TTCTGGAGAT GGAGTGGGGC TCCATGCAGC AGTTCCTCTA CGAGATCTCC
    AATCTGGAGT GTGTGAGTAA CGCCGGGGCG TTCGAGGGCT ACATCGACCT GGGCCGCGAG
    CTCTCCATCC TGCACAGTCT GCTGTGGGAG GTCATGGCCC AGCTCAGCAA GGATGCGATC
    CTGAAGCTGG GTCCTCTGCC GCGGCTGCTG AATGACATCA GTGTGGCCCT GCGTAACCCT
    CAGCTGCACA GACAGGCCAG CCACCAGCCA CCCGAGCGAC ACCTGAGCCG GCCTGCGTTC
    AGCAGACTCG CCACCAATGA CTTCCAGAGC CTCATGATGA GAGACCTCAA CAGCTCCATA
    GACATCTCTC GACTGCCGTC TCCTACAGCC GGTCTGTCCG GCAGCGGTGT TCTCTCTCAC
    CCGGGCATGG CTGCGTTCTC AGAGCGAGAT CCCCGCGGCA ACAAAGACGT CTTCTATGTG
    ACCCGCCCAC CTCTCGCACG CTCAAGCCCC GCCTACTGCA CCAGCAGCTC TGACATCACT
    GAGCCAGACC CCAAGGTACT CAGCGTCAAT AAGAGTGTGT CTATGATGGA CCTGCAGGAC
    TCGCGCATCA ACAGCGTGTC TAACCTGCAC TCAGTGAATG ATCTGCTGAA CTCGTCTCAG
    GGTTCTCTGG CTGGGTTCGG TCCGCTCGGC GGTGCCCCCC TCAGGGCTGG AGGACGCGTG
    TCTGCGGGCT CGGGCGGCTC CAGCATGTCT GGCGGTCTGC GTTTGACCAC AGACTCTCTG
    TCGCAGCAGC AACAGCAGGC CGCCGCCGCC ATGCACGTGC CGCTGTCCTT CCAGAACCCT
    CTGTTCCACC TAGCATCAGA TGGGCCGCAG TATCACACAC CTCCACACGG GCCCACTGCT
    GTACCGCCAC CGCTGCTGCT GGCGCCCGAG CCAGAGAACG GGCATGTGGG TTTCGGCGTT
    CCCACATTCG CCCACAGTGG GTTCTCCCGC AGTGAAGATC TGTCAGCGCT GCGGACGCAG
    AACAGTCTGG TGCAGCCCAA CATCGTGCAC TCGCACAGCT ACAGCGACGA CTTCACACGA
    CACAACCAGG CCGACTACGC CCGCAGACAG CTGTCTCTGC AGGTGCAGGA GAATCTCCAG
    CAGCAGGTGC TGATGGGAAT GACCCCGCAG ACGGCCACAG GCACCGGCAC TCCTGCTTCT
    CTGGCCACAC CTCCCACCAC CATTCATCCT GTCCGTCAGG CCTCCATGGC TCCGCCCCCT
    CCTCAACGCG CGAAGCCTCA GCCCGCCCAC CAGCTGTCCG TCAGCTCGGC TGTAAACTCC
    ACCCCCCCTA AGACCCGCCC ACAGAGCGGC AACCTCCTCC AATCGCCTGA GTCTAGTTTT
    GGAGGGAGGC AGTTGACAAG GCAGCAGCTG TCAGTCAAGG ACAGCCCCGC CCCCGGGTTG
    CCGCATCAGT CCAGCAATGC GTCGGAGAAT CCCGCTCCTC AGAGCGCAGC CAATGAGAGC
    ACAGAGAATA CGCCGACCAA CCAATCAGCA AAAAACACAC AGCAGAGCAA CCAACAACAC
    CTGCTCAAGC CATCCGGGAA CAAACAGGGC TCTAAGTCTC CCTCCACCCT GAACCCCACC
    TCTGCAGCCT CCGAGCGCAC CGTGGCCTGG GTCTCCAACA TGCCACATCT GTCTGCAGAC
    ATCGAGAGTC TGCGCGTGGA CAGAGAGGAG TTCAAACTCA AGGAGCACTC CAAGAGCATG
    GATGAGTCCC GCCTGGACCG GGTGCGTGAG TACGAGGAGG AGATCAACTC CCTGAAGGAG
    CGTCTGATGA TGTCCCACCG TAAGCTGGAG GAGTACGAGC GACGGCTACA CTCGCAGGAG
    CAGCAGACCA GCAAGATTCT GCTGCAGTAC CAGAGCCGGC TGGACGACAG CGAGAGGCGC
    CTGCGGCAGC AGCAGGTGGA GAAGGACTCG CAGATCAAGG GCATCATCAA CAGGTTGATG
    GCGGTAGAGG ATGAGCTGAG GGGAAGTGGA ATGCCTGACC TCAAAACCAG AATATTCACA
    GAACAGCCGA TTTATGGTGC CCACTCTGGA ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23694
    Clone ID Related Accession (Same CDS sequence) XM_003200501.4 , XM_003200501.5
    Accession Version XM_003200501.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras/Rap GTPase-activating protein SynGAP isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003200501.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCTATG TGTCTTCACA AGATGGAAGG TGTGTGTCTC CATGTTCTCG GCCTCACACT 
    CCTCTGAATG GCCACGCACA CGACTCCCCC GGCTGGAGCC GACAGCTCCG CGTCAGAGAT
    GGAGACCAGT TCTACCTGCT GGATCAAGAG GAGGTTCATC CACTGCTGAT GAATGATCAT
    CGTCCCGTCT CTCACCGACA CAAACTGCTG CGCAGGACCG TCAGCGTACC TGCAGATAGC
    AGACCCCACC CTGAGTCAGA GCACGCTCGT GCTCGCAGGA AGAGCTTTGC CACGTCGCGG
    CAGAGAAGCA TGGAAACTCC GCCCACTGCC CCTACACAGC CATTTCGACA GCCTAGTTTT
    CTCAGCCGCC GCTTGAAAGG ATCAATTAAA CGAGCAAAGA GTCAACCCAA ACTCGACCGC
    ACGGGAAGCT TCCGCCACAT GATTCTCCCA CGATTCCGCA GTGCTGACCA GGAGAGGACG
    CGTCTGATGC AGAGCTTTAA GGAGTCTCAC TCTCATGAGT CTCTGCTGTC TCCGAGCAGC
    GCGGCCGAGG CTCTGGACCT CACGCTGGAC GAGGACGCCA TCATCAAACC CGTCCACTCC
    AGCATCCTCG GCCAGGAGTA CTGCTTCGAG GTGACCACGG CGTCAGGAAC CAAGTGTTTC
    GCCTGCCGCT CCGCTGCTGA GAGAGACAAA TGGATCGAGA ATCTGCAGCG AGCCGTCAAA
    CCAAACAAGG ACAACAGTCG TCGTGTGGAT AACGTGCTGA AGTTATGGAT CATCGAGGCT
    CGAGATCTGC CACCGAAGAA GCGCTACTAC TGCGAGCTGT GTCTGGACGA CATGCTGTAC
    GCTCGAACCA CCAGCAAACC GCGCACCGAC ACCGTCTTCT GGGGCGAACA CTTCGAGTTC
    AACAACCTGC CGGCCATCCG CAGCCTCCGA CTGCACCTCT ACAAGGAGAA CGACAAGAAG
    AGGAGGAAGG AGAAGAGCAC GTATCTGGGT CTGGTCAGCA TCCCGATCCC GAGCGTGACG
    GGTCGTCAGT TTGTGGAGCA GTGGTACCCG CTCATCCAGC CCAGTGTTCT GGCTAAAGGC
    GGCGGCGGCG GGGGGAAGAT CATCAACGCC TCGCTGCGCC TCAAGTCCCG CTTCCAGAGC
    ATGAACATCC TGCCCATGGA GCTCTACAAG GAGTTCGCCG AGTACGTCAC CAACAACTAC
    CGCACGCTCT GCGGAGTGCT GGAGCCGCTG CTCAGCGCCA AGAGCAAAGA GGAGGTGGCC
    TGCGCGCTAG TGCACATCCT GCAGAGCACT GGGAAGGCCA AGGACTTCCT GTCTGACATG
    GCCATGTGTG AGGTGGATCG TTTCATGGAC CGTGAGCATC TGATCTTCAG AGAAAACACT
    CTCGCCACTA AAGCCATCGA GGAGTTCCTG AAGCTCATCG CACACCGCTA CCTGAAGGAC
    ACCATCGGAG AGTTCATCCG TGCGCTGTAC GAGTCTGAGG AGAACTGTGA GGTGGACCCG
    ATGCGAACGC CTCCGTCAGT GCTGGCCGAT CATCAGGCTA ACCTGCGCAT GTGCTGTGAG
    CTAGCGCTCT GCAAGATCAT CAACTCTCAC TGCGTGTTTC CCCGTGAGCT GAAGGAGGTG
    TTTGCGTCGT GGCGTGTGCG CTGTGCTGAG CGCGGCCGAG AGGACATCGC CGACCGCCTC
    ATCAGCTCCT CGCTCTTCCT GCGCTTCCTG TGTCCGGCGA TCATGTCCCC GTCCCTCTTC
    AACCTGACGC AGGAGTATCC CTCCGAGCGC ACCTCTCGAA CCCTCACGCT CATCGCCAAG
    GTGGTGCAGA GCCTGGCCAG CTTTTCAAAG TTCAGTGGGA AGGAGGACTA TCTGGGCTTC
    ATGAATGAGT TTCTGGAGAT GGAGTGGGGC TCCATGCAGC AGTTCCTCTA CGAGATCTCC
    AATCTGGAGT GTGTGAGTAA CGCCGGGGCG TTCGAGGGCT ACATCGACCT GGGCCGCGAG
    CTCTCCATCC TGCACAGTCT GCTGTGGGAG GTCATGGCCC AGCTCAGCAA GGATGCGATC
    CTGAAGCTGG GTCCTCTGCC GCGGCTGCTG AATGACATCA GTGTGGCCCT GCGTAACCCT
    CAGCTGCACA GACAGGCCAG CCACCAGCCA CCCGAGCGAC ACCTGAGCCG GCCTGCGTTC
    AGCAGACTCG CCACCAATGA CTTCCAGAGC CTCATGATGA GAGACCTCAA CAGCTCCATA
    GACATCTCTC GACTGCCGTC TCCTACAGCC GGTCTGTCCG GCAGCGGTGT TCTCTCTCAC
    CCGGGCATGG CTGCGTTCTC AGAGCGAGAT CCCCGCGGCA ACAAAGACGT CTTCTATGTG
    ACCCGCCCAC CTCTCGCACG CTCAAGCCCC GCCTACTGCA CCAGCAGCTC TGACATCACT
    GAGCCAGACC CCAAGGTACT CAGCGTCAAT AAGAGTGTGT CTATGATGGA CCTGCAGGAC
    TCGCGCATCA ACAGCGTGTC TAACCTGCAC TCAGTGAATG ATCTGCTGAA CTCGTCTCAG
    GGTTCTCTGG CTGGGTTCGG TCCGCTCGGC GGTGCCCCCC TCAGGGCTGG AGGACGCGTG
    TCTGCGGGCT CGGGCGGCTC CAGCATGTCT GGCGGTCTGC GTTTGACCAC AGACTCTCTG
    TCGCAGCAGC AACAGCAGGC CGCCGCCGCC ATGCACGTGC CGCTGTCCTT CCAGAACCCT
    CTGTTCCACC TAGCATCAGA TGGGCCGCAG TATCACACAC CTCCACACGG GCCCACTGCT
    GTACCGCCAC CGCTGCTGCT GGCGCCCGAG CCAGAGAACG GGCATGTGGG TTTCGGCGTT
    CCCACATTCG CCCACAGTGG GTTCTCCCGC AGTGAAGATC TGTCAGCGCT GCGGACGCAG
    AACAGTCTGG TGCAGCCCAA CATCGTGCAC TCGCACAGCT ACAGCGACGA CTTCACACGA
    CACAACCAGG CCGACTACGC CCGCAGACAG CTGTCTCTGC AGGTGCAGGA GAATCTCCAG
    CAGCAGGTGC TGATGGGAAT GACCCCGCAG ACGGCCACAG GCACCGGCAC TCCTGCTTCT
    CTGGCCACAC CTCCCACCAC CATTCATCCT GTCCGTCAGG CCTCCATGGC TCCGCCCCCT
    CCTCAACGCG CGAAGCCTCA GCCCGCCCAC CAGCTGTCCG TCAGCTCGGC TGTAAACTCC
    ACCCCCCCTA AGACCCGCCC ACAGAGCGGC AACCTCCTCC AATCGCCTGA GTCTAGTTTT
    GGAGGGAGGC AGTTGACAAG GCAGCAGCTG TCAGTCAAGG ACAGCCCCGC CCCCGGGTTG
    CCGCATCAGT CCAGCAATGC GTCGGAGAAT CCCGCTCCTC AGAGCGCAGC CAATGAGAGC
    ACAGAGAATA CGCCGACCAA CCAATCAGCA AAAAACACAC AGCAGAGCAA CCAACAACAC
    CTGCTCAAGC CATCCGGGAA CAAACAGGGC TCTAAGTCTC CCTCCACCCT GAACCCCACC
    TCTGCAGCCT CCGAGCGCAC CGTGGCCTGG GTCTCCAACA TGCCACATCT GTCTGCAGAC
    ATCGAGAGTC TGCGCGTGGA CAGAGAGGAG TTCAAACTCA AGGAGCACTC CAAGAGCATG
    GATGAGTCCC GCCTGGACCG GGTGCGTGAG TACGAGGAGG AGATCAACTC CCTGAAGGAG
    CGTCTGATGA TGTCCCACCG TAAGCTGGAG GAGTACGAGC GACGGCTACA CTCGCAGGAG
    CAGCAGACCA GCAAGATTCT GCTGCAGTAC CAGAGCCGGC TGGACGACAG CGAGAGGCGC
    CTGCGGCAGC AGCAGGTGGA GAAGGACTCG CAGATCAAGG GCATCATCAA CAGGTTGATG
    GCGGTAGAGG ATGAGCTGAG GGGAAGTGGA ATGCCTGACC TCAAAACCAG AATATTCACA
    GAACAGCCGA TTTATGGTGC CCACTCTGGA ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003200549.2
    CDS175..4110
    Translation

    Target ORF information:

    RefSeq Version XM_003200501.5
    Organism Danio rerio(zebrafish)
    Definition Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003200501.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCTATG TGTCTTCACA AGATGGAAGG TGTGTGTCTC CATGTTCTCG GCCTCACACT 
    CCTCTGAATG GCCACGCACA CGACTCCCCC GGCTGGAGCC GACAGCTCCG CGTCAGAGAT
    GGAGACCAGT TCTACCTGCT GGATCAAGAG GAGGTTCATC CACTGCTGAT GAATGATCAT
    CGTCCCGTCT CTCACCGACA CAAACTGCTG CGCAGGACCG TCAGCGTACC TGCAGATAGC
    AGACCCCACC CTGAGTCAGA GCACGCTCGT GCTCGCAGGA AGAGCTTTGC CACGTCGCGG
    CAGAGAAGCA TGGAAACTCC GCCCACTGCC CCTACACAGC CATTTCGACA GCCTAGTTTT
    CTCAGCCGCC GCTTGAAAGG ATCAATTAAA CGAGCAAAGA GTCAACCCAA ACTCGACCGC
    ACGGGAAGCT TCCGCCACAT GATTCTCCCA CGATTCCGCA GTGCTGACCA GGAGAGGACG
    CGTCTGATGC AGAGCTTTAA GGAGTCTCAC TCTCATGAGT CTCTGCTGTC TCCGAGCAGC
    GCGGCCGAGG CTCTGGACCT CACGCTGGAC GAGGACGCCA TCATCAAACC CGTCCACTCC
    AGCATCCTCG GCCAGGAGTA CTGCTTCGAG GTGACCACGG CGTCAGGAAC CAAGTGTTTC
    GCCTGCCGCT CCGCTGCTGA GAGAGACAAA TGGATCGAGA ATCTGCAGCG AGCCGTCAAA
    CCAAACAAGG ACAACAGTCG TCGTGTGGAT AACGTGCTGA AGTTATGGAT CATCGAGGCT
    CGAGATCTGC CACCGAAGAA GCGCTACTAC TGCGAGCTGT GTCTGGACGA CATGCTGTAC
    GCTCGAACCA CCAGCAAACC GCGCACCGAC ACCGTCTTCT GGGGCGAACA CTTCGAGTTC
    AACAACCTGC CGGCCATCCG CAGCCTCCGA CTGCACCTCT ACAAGGAGAA CGACAAGAAG
    AGGAGGAAGG AGAAGAGCAC GTATCTGGGT CTGGTCAGCA TCCCGATCCC GAGCGTGACG
    GGTCGTCAGT TTGTGGAGCA GTGGTACCCG CTCATCCAGC CCAGTGTTCT GGCTAAAGGC
    GGCGGCGGCG GGGGGAAGAT CATCAACGCC TCGCTGCGCC TCAAGTCCCG CTTCCAGAGC
    ATGAACATCC TGCCCATGGA GCTCTACAAG GAGTTCGCCG AGTACGTCAC CAACAACTAC
    CGCACGCTCT GCGGAGTGCT GGAGCCGCTG CTCAGCGCCA AGAGCAAAGA GGAGGTGGCC
    TGCGCGCTAG TGCACATCCT GCAGAGCACT GGGAAGGCCA AGGACTTCCT GTCTGACATG
    GCCATGTGTG AGGTGGATCG TTTCATGGAC CGTGAGCATC TGATCTTCAG AGAAAACACT
    CTCGCCACTA AAGCCATCGA GGAGTTCCTG AAGCTCATCG CACACCGCTA CCTGAAGGAC
    ACCATCGGAG AGTTCATCCG TGCGCTGTAC GAGTCTGAGG AGAACTGTGA GGTGGACCCG
    ATGCGAACGC CTCCGTCAGT GCTGGCCGAT CATCAGGCTA ACCTGCGCAT GTGCTGTGAG
    CTAGCGCTCT GCAAGATCAT CAACTCTCAC TGCGTGTTTC CCCGTGAGCT GAAGGAGGTG
    TTTGCGTCGT GGCGTGTGCG CTGTGCTGAG CGCGGCCGAG AGGACATCGC CGACCGCCTC
    ATCAGCTCCT CGCTCTTCCT GCGCTTCCTG TGTCCGGCGA TCATGTCCCC GTCCCTCTTC
    AACCTGACGC AGGAGTATCC CTCCGAGCGC ACCTCTCGAA CCCTCACGCT CATCGCCAAG
    GTGGTGCAGA GCCTGGCCAG CTTTTCAAAG TTCAGTGGGA AGGAGGACTA TCTGGGCTTC
    ATGAATGAGT TTCTGGAGAT GGAGTGGGGC TCCATGCAGC AGTTCCTCTA CGAGATCTCC
    AATCTGGAGT GTGTGAGTAA CGCCGGGGCG TTCGAGGGCT ACATCGACCT GGGCCGCGAG
    CTCTCCATCC TGCACAGTCT GCTGTGGGAG GTCATGGCCC AGCTCAGCAA GGATGCGATC
    CTGAAGCTGG GTCCTCTGCC GCGGCTGCTG AATGACATCA GTGTGGCCCT GCGTAACCCT
    CAGCTGCACA GACAGGCCAG CCACCAGCCA CCCGAGCGAC ACCTGAGCCG GCCTGCGTTC
    AGCAGACTCG CCACCAATGA CTTCCAGAGC CTCATGATGA GAGACCTCAA CAGCTCCATA
    GACATCTCTC GACTGCCGTC TCCTACAGCC GGTCTGTCCG GCAGCGGTGT TCTCTCTCAC
    CCGGGCATGG CTGCGTTCTC AGAGCGAGAT CCCCGCGGCA ACAAAGACGT CTTCTATGTG
    ACCCGCCCAC CTCTCGCACG CTCAAGCCCC GCCTACTGCA CCAGCAGCTC TGACATCACT
    GAGCCAGACC CCAAGGTACT CAGCGTCAAT AAGAGTGTGT CTATGATGGA CCTGCAGGAC
    TCGCGCATCA ACAGCGTGTC TAACCTGCAC TCAGTGAATG ATCTGCTGAA CTCGTCTCAG
    GGTTCTCTGG CTGGGTTCGG TCCGCTCGGC GGTGCCCCCC TCAGGGCTGG AGGACGCGTG
    TCTGCGGGCT CGGGCGGCTC CAGCATGTCT GGCGGTCTGC GTTTGACCAC AGACTCTCTG
    TCGCAGCAGC AACAGCAGGC CGCCGCCGCC ATGCACGTGC CGCTGTCCTT CCAGAACCCT
    CTGTTCCACC TAGCATCAGA TGGGCCGCAG TATCACACAC CTCCACACGG GCCCACTGCT
    GTACCGCCAC CGCTGCTGCT GGCGCCCGAG CCAGAGAACG GGCATGTGGG TTTCGGCGTT
    CCCACATTCG CCCACAGTGG GTTCTCCCGC AGTGAAGATC TGTCAGCGCT GCGGACGCAG
    AACAGTCTGG TGCAGCCCAA CATCGTGCAC TCGCACAGCT ACAGCGACGA CTTCACACGA
    CACAACCAGG CCGACTACGC CCGCAGACAG CTGTCTCTGC AGGTGCAGGA GAATCTCCAG
    CAGCAGGTGC TGATGGGAAT GACCCCGCAG ACGGCCACAG GCACCGGCAC TCCTGCTTCT
    CTGGCCACAC CTCCCACCAC CATTCATCCT GTCCGTCAGG CCTCCATGGC TCCGCCCCCT
    CCTCAACGCG CGAAGCCTCA GCCCGCCCAC CAGCTGTCCG TCAGCTCGGC TGTAAACTCC
    ACCCCCCCTA AGACCCGCCC ACAGAGCGGC AACCTCCTCC AATCGCCTGA GTCTAGTTTT
    GGAGGGAGGC AGTTGACAAG GCAGCAGCTG TCAGTCAAGG ACAGCCCCGC CCCCGGGTTG
    CCGCATCAGT CCAGCAATGC GTCGGAGAAT CCCGCTCCTC AGAGCGCAGC CAATGAGAGC
    ACAGAGAATA CGCCGACCAA CCAATCAGCA AAAAACACAC AGCAGAGCAA CCAACAACAC
    CTGCTCAAGC CATCCGGGAA CAAACAGGGC TCTAAGTCTC CCTCCACCCT GAACCCCACC
    TCTGCAGCCT CCGAGCGCAC CGTGGCCTGG GTCTCCAACA TGCCACATCT GTCTGCAGAC
    ATCGAGAGTC TGCGCGTGGA CAGAGAGGAG TTCAAACTCA AGGAGCACTC CAAGAGCATG
    GATGAGTCCC GCCTGGACCG GGTGCGTGAG TACGAGGAGG AGATCAACTC CCTGAAGGAG
    CGTCTGATGA TGTCCCACCG TAAGCTGGAG GAGTACGAGC GACGGCTACA CTCGCAGGAG
    CAGCAGACCA GCAAGATTCT GCTGCAGTAC CAGAGCCGGC TGGACGACAG CGAGAGGCGC
    CTGCGGCAGC AGCAGGTGGA GAAGGACTCG CAGATCAAGG GCATCATCAA CAGGTTGATG
    GCGGTAGAGG ATGAGCTGAG GGGAAGTGGA ATGCCTGACC TCAAAACCAG AATATTCACA
    GAACAGCCGA TTTATGGTGC CCACTCTGGA ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23695
    Clone ID Related Accession (Same CDS sequence) XM_005159317.3 , XM_005159317.4
    Accession Version XM_005159317.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ras/Rap GTPase-activating protein SynGAP isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159317.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCCTATG TGTCTTCACA AGATGGAAGG TGTGTGTCTC CATGTTCTCG GCCTCACACT 
    CCTCTGAATG GCCACGCACA CGACTCCCCC GGCTGGAGCC GACAGCTCCG CGTCAGAGAT
    GGAGACCAGT TCTACCTGCT GGATCAAGAG GAGGTTCATC CACTGCTGAT GAATGATCAT
    CGTCCCGTCT CTCACCGACA CAAACTGCTG CGCAGGACCG TCAGCGTACC TGCAGATAGC
    AGACCCCACC CTGAGTCAGA GCACGCTCGT GCTCGCAGGA AGAGCTTTGC CACGTCGCGG
    CAGAGAAGCA TGGAAACTCC GCCCACTGCC CCTACACAGC CATTTCGACA GCCTAGTTTT
    CTCAGCCGCC GCTTGAAAGG ATCAATTAAA CGAGCAAAGA GTCAACCCAA ACTCGACCGC
    ACGGGAAGCT TCCGCCACAT GATTCTCCCA CGATTCCGCA GTGCTGACCA GGAGAGGACG
    CGTCTGATGC AGAGCTTTAA GGAGTCTCAC TCTCATGAGT CTCTGCTGTC TCCGAGCAGC
    GCGGCCGAGG CTCTGGACCT CACGCTGGAC GAGGACGCCA TCATCAAACC CGTCCACTCC
    AGCATCCTCG GCCAGGAGTA CTGCTTCGAG GTGACCACGG CGTCAGGAAC CAAGTGTTTC
    GCCTGCCGCT CCGCTGCTGA GAGAGACAAA TGGATCGAGA ATCTGCAGCG AGCCGTCAAA
    CCAAACAAGG ACAACAGTCG TCGTGTGGAT AACGTGCTGA AGTTATGGAT CATCGAGGCT
    CGAGATCTGC CACCGAAGAA GCGCTACTAC TGCGAGCTGT GTCTGGACGA CATGCTGTAC
    GCTCGAACCA CCAGCAAACC GCGCACCGAC ACCGTCTTCT GGGGCGAACA CTTCGAGTTC
    AACAACCTGC CGGCCATCCG CAGCCTCCGA CTGCACCTCT ACAAGGAGAA CGACAAGAAG
    AGGAGGAAGG AGAAGAGCAC GTATCTGGGT CTGGTCAGCA TCCCGATCCC GAGCGTGACG
    GGTCGTCAGT TTGTGGAGCA GTGGTACCCG CTCATCCAGC CCAGTGTTCT GGCTAAAGGC
    GGCGGCGGCG GGGGGAAGAT CATCAACGCC TCGCTGCGCC TCAAGTCCCG CTTCCAGAGC
    ATGAACATCC TGCCCATGGA GCTCTACAAG GAGTTCGCCG AGTACGTCAC CAACAACTAC
    CGCACGCTCT GCGGAGTGCT GGAGCCGCTG CTCAGCGCCA AGAGCAAAGA GGAGGTGGCC
    TGCGCGCTAG TGCACATCCT GCAGAGCACT GGGAAGGCCA AGGACTTCCT GTCTGACATG
    GCCATGTGTG AGGTGGATCG TTTCATGGAC CGTGAGCATC TGATCTTCAG AGAAAACACT
    CTCGCCACTA AAGCCATCGA GGAGTTCCTG AAGCTCATCG CACACCGCTA CCTGAAGGAC
    ACCATCGGTG AGGGAGAGTT CATCCGTGCG CTGTACGAGT CTGAGGAGAA CTGTGAGGTG
    GACCCGATGC GAACGCCTCC GTCAGTGCTG GCCGATCATC AGGCTAACCT GCGCATGTGC
    TGTGAGCTAG CGCTCTGCAA GATCATCAAC TCTCACTGCG TGTTTCCCCG TGAGCTGAAG
    GAGGTGTTTG CGTCGTGGCG TGTGCGCTGT GCTGAGCGCG GCCGAGAGGA CATCGCCGAC
    CGCCTCATCA GCTCCTCGCT CTTCCTGCGC TTCCTGTGTC CGGCGATCAT GTCCCCGTCC
    CTCTTCAACC TGACGCAGGA GTATCCCTCC GAGCGCACCT CTCGAACCCT CACGCTCATC
    GCCAAGGTGG TGCAGAGCCT GGCCAGCTTT TCAAAGTTCA GTGGGAAGGA GGACTATCTG
    GGCTTCATGA ATGAGTTTCT GGAGATGGAG TGGGGCTCCA TGCAGCAGTT CCTCTACGAG
    ATCTCCAATC TGGAGTGTGT GAGTAACGCC GGGGCGTTCG AGGGCTACAT CGACCTGGGC
    CGCGAGCTCT CCATCCTGCA CAGTCTGCTG TGGGAGGTCA TGGCCCAGCT CAGCAAGGAT
    GCGATCCTGA AGCTGGGTCC TCTGCCGCGG CTGCTGAATG ACATCAGTGT GGCCCTGCGT
    AACCCTCAGC TGCACAGACA GGCCAGCCAC CAGCCACCCG AGCGACACCT GAGCCGGCCT
    GCGTTCAGCA GACTCGCCAC CAATGACTTC CAGAGCCTCA TGATGAGAGA CCTCAACAGC
    TCCATAGACA TCTCTCGACT GCCGTCTCCT ACAGCCGGTC TGTCCGGCAG CGGTGTTCTC
    TCTCACCCGG GCATGGCTGC GTTCTCAGAG CGAGATCCCC GCGGCAACAA AGACGTCTTC
    TATGTGACCC GCCCACCTCT CGCACGCTCA AGCCCCGCCT ACTGCACCAG CAGCTCTGAC
    ATCACTGAGC CAGACCCCAA GGTACTCAGC GTCAATAAGA GTGTGTCTAT GATGGACCTG
    CAGGACTCGC GCATCAACAG CGTGTCTAAC CTGCACTCAG TGAATGATCT GCTGAACTCG
    TCTCAGGGTT CTCTGGCTGG GTTCGGTCCG CTCGGCGGTG CCCCCCTCAG GGCTGGAGGA
    CGCGTGTCTG CGGGCTCGGG CGGCTCCAGC ATGTCTGGCG GTCTGCGTTT GACCACAGAC
    TCTCTGTCGC AGCAGCAACA GCAGGCCGCC GCCGCCATGC ACGTGCCGCT GTCCTTCCAG
    AACCCTCTGT TCCACCTAGC ATCAGATGGG CCGCAGTATC ACACACCTCC ACACGGGCCC
    ACTGCTGTAC CGCCACCGCT GCTGCTGGCG CCCGAGCCAG AGAACGGGCA TGTGGGTTTC
    GGCGTTCCCA CATTCGCCCA CAGTGGGTTC TCCCGCAGTG AAGATCTGTC AGCGCTGCGG
    ACGCAGAACA GTCTGGTGCA GCCCAACATC GTGCACTCGC ACAGCTACAG CGACGACTTC
    ACACGACACA ACCAGGCCGA CTACGCCCGC AGACAGCTGT CTCTGCAGGT GCAGGAGAAT
    CTCCAGCAGC AGGTGCTGAT GGGAATGACC CCGCAGACGG CCACAGGCAC CGGCACTCCT
    GCTTCTCTGG CCACACCTCC CACCACCATT CATCCTGTCC GTCAGGCCTC CATGGCTCCG
    CCCCCTCCTC AACGCGCGAA GCCTCAGCCC GCCCACCAGC TGTCCGTCAG CTCGGCTGTA
    AACTCCACCC CCCCTAAGAC CCGCCCACAG AGCGGCAACC TCCTCCAATC GCCTGAGTCT
    AGTTTTGGAG GGAGGCAGTT GACAAGGCAG CAGCTGTCAG TCAAGGACAG CCCCGCCCCC
    GGGTTGCCGC ATCAGTCCAG CAATGCGTCG GAGAATCCCG CTCCTCAGAG CGCAGCCAAT
    GAGAGCACAG AGAATACGCC GACCAACCAA TCAGCAAAAA ACACACAGCA GAGCAACCAA
    CAACACCTGC TCAAGCCATC CGGGAACAAA CAGGGCTCTA AGTCTCCCTC CACCCTGAAC
    CCCACCTCTG CAGCCTCCGA GCGCACCGTG GCCTGGGTCT CCAACATGCC ACATCTGTCT
    GCAGACATCG AGAGTCTGCG CGTGGACAGA GAGGAGTTCA AACTCAAGGA GCACTCCAAG
    AGCATGGATG AGTCCCGCCT GGACCGGGTG CGTGAGTACG AGGAGGAGAT CAACTCCCTG
    AAGGAGCGTC TGATGATGTC CCACCGTAAG CTGGAGGAGT ACGAGCGACG GCTACACTCG
    CAGGAGCAGC AGACCAGCAA GATTCTGCTG CAGTACCAGA GCCGGCTGGA CGACAGCGAG
    AGGCGCCTGC GGCAGCAGCA GGTGGAGAAG GACTCGCAGA TCAAGGGCAT CATCAACAGC
    CGATTTATGG TGCCCACTCT GGAACATGAT TGGCCCCTGC AGGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159374.1
    CDS175..4062
    Translation

    Target ORF information:

    RefSeq Version XM_005159317.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159317.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCCTATG TGTCTTCACA AGATGGAAGG TGTGTGTCTC CATGTTCTCG GCCTCACACT 
    CCTCTGAATG GCCACGCACA CGACTCCCCC GGCTGGAGCC GACAGCTCCG CGTCAGAGAT
    GGAGACCAGT TCTACCTGCT GGATCAAGAG GAGGTTCATC CACTGCTGAT GAATGATCAT
    CGTCCCGTCT CTCACCGACA CAAACTGCTG CGCAGGACCG TCAGCGTACC TGCAGATAGC
    AGACCCCACC CTGAGTCAGA GCACGCTCGT GCTCGCAGGA AGAGCTTTGC CACGTCGCGG
    CAGAGAAGCA TGGAAACTCC GCCCACTGCC CCTACACAGC CATTTCGACA GCCTAGTTTT
    CTCAGCCGCC GCTTGAAAGG ATCAATTAAA CGAGCAAAGA GTCAACCCAA ACTCGACCGC
    ACGGGAAGCT TCCGCCACAT GATTCTCCCA CGATTCCGCA GTGCTGACCA GGAGAGGACG
    CGTCTGATGC AGAGCTTTAA GGAGTCTCAC TCTCATGAGT CTCTGCTGTC TCCGAGCAGC
    GCGGCCGAGG CTCTGGACCT CACGCTGGAC GAGGACGCCA TCATCAAACC CGTCCACTCC
    AGCATCCTCG GCCAGGAGTA CTGCTTCGAG GTGACCACGG CGTCAGGAAC CAAGTGTTTC
    GCCTGCCGCT CCGCTGCTGA GAGAGACAAA TGGATCGAGA ATCTGCAGCG AGCCGTCAAA
    CCAAACAAGG ACAACAGTCG TCGTGTGGAT AACGTGCTGA AGTTATGGAT CATCGAGGCT
    CGAGATCTGC CACCGAAGAA GCGCTACTAC TGCGAGCTGT GTCTGGACGA CATGCTGTAC
    GCTCGAACCA CCAGCAAACC GCGCACCGAC ACCGTCTTCT GGGGCGAACA CTTCGAGTTC
    AACAACCTGC CGGCCATCCG CAGCCTCCGA CTGCACCTCT ACAAGGAGAA CGACAAGAAG
    AGGAGGAAGG AGAAGAGCAC GTATCTGGGT CTGGTCAGCA TCCCGATCCC GAGCGTGACG
    GGTCGTCAGT TTGTGGAGCA GTGGTACCCG CTCATCCAGC CCAGTGTTCT GGCTAAAGGC
    GGCGGCGGCG GGGGGAAGAT CATCAACGCC TCGCTGCGCC TCAAGTCCCG CTTCCAGAGC
    ATGAACATCC TGCCCATGGA GCTCTACAAG GAGTTCGCCG AGTACGTCAC CAACAACTAC
    CGCACGCTCT GCGGAGTGCT GGAGCCGCTG CTCAGCGCCA AGAGCAAAGA GGAGGTGGCC
    TGCGCGCTAG TGCACATCCT GCAGAGCACT GGGAAGGCCA AGGACTTCCT GTCTGACATG
    GCCATGTGTG AGGTGGATCG TTTCATGGAC CGTGAGCATC TGATCTTCAG AGAAAACACT
    CTCGCCACTA AAGCCATCGA GGAGTTCCTG AAGCTCATCG CACACCGCTA CCTGAAGGAC
    ACCATCGGTG AGGGAGAGTT CATCCGTGCG CTGTACGAGT CTGAGGAGAA CTGTGAGGTG
    GACCCGATGC GAACGCCTCC GTCAGTGCTG GCCGATCATC AGGCTAACCT GCGCATGTGC
    TGTGAGCTAG CGCTCTGCAA GATCATCAAC TCTCACTGCG TGTTTCCCCG TGAGCTGAAG
    GAGGTGTTTG CGTCGTGGCG TGTGCGCTGT GCTGAGCGCG GCCGAGAGGA CATCGCCGAC
    CGCCTCATCA GCTCCTCGCT CTTCCTGCGC TTCCTGTGTC CGGCGATCAT GTCCCCGTCC
    CTCTTCAACC TGACGCAGGA GTATCCCTCC GAGCGCACCT CTCGAACCCT CACGCTCATC
    GCCAAGGTGG TGCAGAGCCT GGCCAGCTTT TCAAAGTTCA GTGGGAAGGA GGACTATCTG
    GGCTTCATGA ATGAGTTTCT GGAGATGGAG TGGGGCTCCA TGCAGCAGTT CCTCTACGAG
    ATCTCCAATC TGGAGTGTGT GAGTAACGCC GGGGCGTTCG AGGGCTACAT CGACCTGGGC
    CGCGAGCTCT CCATCCTGCA CAGTCTGCTG TGGGAGGTCA TGGCCCAGCT CAGCAAGGAT
    GCGATCCTGA AGCTGGGTCC TCTGCCGCGG CTGCTGAATG ACATCAGTGT GGCCCTGCGT
    AACCCTCAGC TGCACAGACA GGCCAGCCAC CAGCCACCCG AGCGACACCT GAGCCGGCCT
    GCGTTCAGCA GACTCGCCAC CAATGACTTC CAGAGCCTCA TGATGAGAGA CCTCAACAGC
    TCCATAGACA TCTCTCGACT GCCGTCTCCT ACAGCCGGTC TGTCCGGCAG CGGTGTTCTC
    TCTCACCCGG GCATGGCTGC GTTCTCAGAG CGAGATCCCC GCGGCAACAA AGACGTCTTC
    TATGTGACCC GCCCACCTCT CGCACGCTCA AGCCCCGCCT ACTGCACCAG CAGCTCTGAC
    ATCACTGAGC CAGACCCCAA GGTACTCAGC GTCAATAAGA GTGTGTCTAT GATGGACCTG
    CAGGACTCGC GCATCAACAG CGTGTCTAAC CTGCACTCAG TGAATGATCT GCTGAACTCG
    TCTCAGGGTT CTCTGGCTGG GTTCGGTCCG CTCGGCGGTG CCCCCCTCAG GGCTGGAGGA
    CGCGTGTCTG CGGGCTCGGG CGGCTCCAGC ATGTCTGGCG GTCTGCGTTT GACCACAGAC
    TCTCTGTCGC AGCAGCAACA GCAGGCCGCC GCCGCCATGC ACGTGCCGCT GTCCTTCCAG
    AACCCTCTGT TCCACCTAGC ATCAGATGGG CCGCAGTATC ACACACCTCC ACACGGGCCC
    ACTGCTGTAC CGCCACCGCT GCTGCTGGCG CCCGAGCCAG AGAACGGGCA TGTGGGTTTC
    GGCGTTCCCA CATTCGCCCA CAGTGGGTTC TCCCGCAGTG AAGATCTGTC AGCGCTGCGG
    ACGCAGAACA GTCTGGTGCA GCCCAACATC GTGCACTCGC ACAGCTACAG CGACGACTTC
    ACACGACACA ACCAGGCCGA CTACGCCCGC AGACAGCTGT CTCTGCAGGT GCAGGAGAAT
    CTCCAGCAGC AGGTGCTGAT GGGAATGACC CCGCAGACGG CCACAGGCAC CGGCACTCCT
    GCTTCTCTGG CCACACCTCC CACCACCATT CATCCTGTCC GTCAGGCCTC CATGGCTCCG
    CCCCCTCCTC AACGCGCGAA GCCTCAGCCC GCCCACCAGC TGTCCGTCAG CTCGGCTGTA
    AACTCCACCC CCCCTAAGAC CCGCCCACAG AGCGGCAACC TCCTCCAATC GCCTGAGTCT
    AGTTTTGGAG GGAGGCAGTT GACAAGGCAG CAGCTGTCAG TCAAGGACAG CCCCGCCCCC
    GGGTTGCCGC ATCAGTCCAG CAATGCGTCG GAGAATCCCG CTCCTCAGAG CGCAGCCAAT
    GAGAGCACAG AGAATACGCC GACCAACCAA TCAGCAAAAA ACACACAGCA GAGCAACCAA
    CAACACCTGC TCAAGCCATC CGGGAACAAA CAGGGCTCTA AGTCTCCCTC CACCCTGAAC
    CCCACCTCTG CAGCCTCCGA GCGCACCGTG GCCTGGGTCT CCAACATGCC ACATCTGTCT
    GCAGACATCG AGAGTCTGCG CGTGGACAGA GAGGAGTTCA AACTCAAGGA GCACTCCAAG
    AGCATGGATG AGTCCCGCCT GGACCGGGTG CGTGAGTACG AGGAGGAGAT CAACTCCCTG
    AAGGAGCGTC TGATGATGTC CCACCGTAAG CTGGAGGAGT ACGAGCGACG GCTACACTCG
    CAGGAGCAGC AGACCAGCAA GATTCTGCTG CAGTACCAGA GCCGGCTGGA CGACAGCGAG
    AGGCGCCTGC GGCAGCAGCA GGTGGAGAAG GACTCGCAGA TCAAGGGCAT CATCAACAGC
    CGATTTATGG TGCCCACTCT GGAACATGAT TGGCCCCTGC AGGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23695
    Clone ID Related Accession (Same CDS sequence) XM_005159317.3 , XM_005159317.4
    Accession Version XM_005159317.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras/Rap GTPase-activating protein SynGAP isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159317.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCCTATG TGTCTTCACA AGATGGAAGG TGTGTGTCTC CATGTTCTCG GCCTCACACT 
    CCTCTGAATG GCCACGCACA CGACTCCCCC GGCTGGAGCC GACAGCTCCG CGTCAGAGAT
    GGAGACCAGT TCTACCTGCT GGATCAAGAG GAGGTTCATC CACTGCTGAT GAATGATCAT
    CGTCCCGTCT CTCACCGACA CAAACTGCTG CGCAGGACCG TCAGCGTACC TGCAGATAGC
    AGACCCCACC CTGAGTCAGA GCACGCTCGT GCTCGCAGGA AGAGCTTTGC CACGTCGCGG
    CAGAGAAGCA TGGAAACTCC GCCCACTGCC CCTACACAGC CATTTCGACA GCCTAGTTTT
    CTCAGCCGCC GCTTGAAAGG ATCAATTAAA CGAGCAAAGA GTCAACCCAA ACTCGACCGC
    ACGGGAAGCT TCCGCCACAT GATTCTCCCA CGATTCCGCA GTGCTGACCA GGAGAGGACG
    CGTCTGATGC AGAGCTTTAA GGAGTCTCAC TCTCATGAGT CTCTGCTGTC TCCGAGCAGC
    GCGGCCGAGG CTCTGGACCT CACGCTGGAC GAGGACGCCA TCATCAAACC CGTCCACTCC
    AGCATCCTCG GCCAGGAGTA CTGCTTCGAG GTGACCACGG CGTCAGGAAC CAAGTGTTTC
    GCCTGCCGCT CCGCTGCTGA GAGAGACAAA TGGATCGAGA ATCTGCAGCG AGCCGTCAAA
    CCAAACAAGG ACAACAGTCG TCGTGTGGAT AACGTGCTGA AGTTATGGAT CATCGAGGCT
    CGAGATCTGC CACCGAAGAA GCGCTACTAC TGCGAGCTGT GTCTGGACGA CATGCTGTAC
    GCTCGAACCA CCAGCAAACC GCGCACCGAC ACCGTCTTCT GGGGCGAACA CTTCGAGTTC
    AACAACCTGC CGGCCATCCG CAGCCTCCGA CTGCACCTCT ACAAGGAGAA CGACAAGAAG
    AGGAGGAAGG AGAAGAGCAC GTATCTGGGT CTGGTCAGCA TCCCGATCCC GAGCGTGACG
    GGTCGTCAGT TTGTGGAGCA GTGGTACCCG CTCATCCAGC CCAGTGTTCT GGCTAAAGGC
    GGCGGCGGCG GGGGGAAGAT CATCAACGCC TCGCTGCGCC TCAAGTCCCG CTTCCAGAGC
    ATGAACATCC TGCCCATGGA GCTCTACAAG GAGTTCGCCG AGTACGTCAC CAACAACTAC
    CGCACGCTCT GCGGAGTGCT GGAGCCGCTG CTCAGCGCCA AGAGCAAAGA GGAGGTGGCC
    TGCGCGCTAG TGCACATCCT GCAGAGCACT GGGAAGGCCA AGGACTTCCT GTCTGACATG
    GCCATGTGTG AGGTGGATCG TTTCATGGAC CGTGAGCATC TGATCTTCAG AGAAAACACT
    CTCGCCACTA AAGCCATCGA GGAGTTCCTG AAGCTCATCG CACACCGCTA CCTGAAGGAC
    ACCATCGGTG AGGGAGAGTT CATCCGTGCG CTGTACGAGT CTGAGGAGAA CTGTGAGGTG
    GACCCGATGC GAACGCCTCC GTCAGTGCTG GCCGATCATC AGGCTAACCT GCGCATGTGC
    TGTGAGCTAG CGCTCTGCAA GATCATCAAC TCTCACTGCG TGTTTCCCCG TGAGCTGAAG
    GAGGTGTTTG CGTCGTGGCG TGTGCGCTGT GCTGAGCGCG GCCGAGAGGA CATCGCCGAC
    CGCCTCATCA GCTCCTCGCT CTTCCTGCGC TTCCTGTGTC CGGCGATCAT GTCCCCGTCC
    CTCTTCAACC TGACGCAGGA GTATCCCTCC GAGCGCACCT CTCGAACCCT CACGCTCATC
    GCCAAGGTGG TGCAGAGCCT GGCCAGCTTT TCAAAGTTCA GTGGGAAGGA GGACTATCTG
    GGCTTCATGA ATGAGTTTCT GGAGATGGAG TGGGGCTCCA TGCAGCAGTT CCTCTACGAG
    ATCTCCAATC TGGAGTGTGT GAGTAACGCC GGGGCGTTCG AGGGCTACAT CGACCTGGGC
    CGCGAGCTCT CCATCCTGCA CAGTCTGCTG TGGGAGGTCA TGGCCCAGCT CAGCAAGGAT
    GCGATCCTGA AGCTGGGTCC TCTGCCGCGG CTGCTGAATG ACATCAGTGT GGCCCTGCGT
    AACCCTCAGC TGCACAGACA GGCCAGCCAC CAGCCACCCG AGCGACACCT GAGCCGGCCT
    GCGTTCAGCA GACTCGCCAC CAATGACTTC CAGAGCCTCA TGATGAGAGA CCTCAACAGC
    TCCATAGACA TCTCTCGACT GCCGTCTCCT ACAGCCGGTC TGTCCGGCAG CGGTGTTCTC
    TCTCACCCGG GCATGGCTGC GTTCTCAGAG CGAGATCCCC GCGGCAACAA AGACGTCTTC
    TATGTGACCC GCCCACCTCT CGCACGCTCA AGCCCCGCCT ACTGCACCAG CAGCTCTGAC
    ATCACTGAGC CAGACCCCAA GGTACTCAGC GTCAATAAGA GTGTGTCTAT GATGGACCTG
    CAGGACTCGC GCATCAACAG CGTGTCTAAC CTGCACTCAG TGAATGATCT GCTGAACTCG
    TCTCAGGGTT CTCTGGCTGG GTTCGGTCCG CTCGGCGGTG CCCCCCTCAG GGCTGGAGGA
    CGCGTGTCTG CGGGCTCGGG CGGCTCCAGC ATGTCTGGCG GTCTGCGTTT GACCACAGAC
    TCTCTGTCGC AGCAGCAACA GCAGGCCGCC GCCGCCATGC ACGTGCCGCT GTCCTTCCAG
    AACCCTCTGT TCCACCTAGC ATCAGATGGG CCGCAGTATC ACACACCTCC ACACGGGCCC
    ACTGCTGTAC CGCCACCGCT GCTGCTGGCG CCCGAGCCAG AGAACGGGCA TGTGGGTTTC
    GGCGTTCCCA CATTCGCCCA CAGTGGGTTC TCCCGCAGTG AAGATCTGTC AGCGCTGCGG
    ACGCAGAACA GTCTGGTGCA GCCCAACATC GTGCACTCGC ACAGCTACAG CGACGACTTC
    ACACGACACA ACCAGGCCGA CTACGCCCGC AGACAGCTGT CTCTGCAGGT GCAGGAGAAT
    CTCCAGCAGC AGGTGCTGAT GGGAATGACC CCGCAGACGG CCACAGGCAC CGGCACTCCT
    GCTTCTCTGG CCACACCTCC CACCACCATT CATCCTGTCC GTCAGGCCTC CATGGCTCCG
    CCCCCTCCTC AACGCGCGAA GCCTCAGCCC GCCCACCAGC TGTCCGTCAG CTCGGCTGTA
    AACTCCACCC CCCCTAAGAC CCGCCCACAG AGCGGCAACC TCCTCCAATC GCCTGAGTCT
    AGTTTTGGAG GGAGGCAGTT GACAAGGCAG CAGCTGTCAG TCAAGGACAG CCCCGCCCCC
    GGGTTGCCGC ATCAGTCCAG CAATGCGTCG GAGAATCCCG CTCCTCAGAG CGCAGCCAAT
    GAGAGCACAG AGAATACGCC GACCAACCAA TCAGCAAAAA ACACACAGCA GAGCAACCAA
    CAACACCTGC TCAAGCCATC CGGGAACAAA CAGGGCTCTA AGTCTCCCTC CACCCTGAAC
    CCCACCTCTG CAGCCTCCGA GCGCACCGTG GCCTGGGTCT CCAACATGCC ACATCTGTCT
    GCAGACATCG AGAGTCTGCG CGTGGACAGA GAGGAGTTCA AACTCAAGGA GCACTCCAAG
    AGCATGGATG AGTCCCGCCT GGACCGGGTG CGTGAGTACG AGGAGGAGAT CAACTCCCTG
    AAGGAGCGTC TGATGATGTC CCACCGTAAG CTGGAGGAGT ACGAGCGACG GCTACACTCG
    CAGGAGCAGC AGACCAGCAA GATTCTGCTG CAGTACCAGA GCCGGCTGGA CGACAGCGAG
    AGGCGCCTGC GGCAGCAGCA GGTGGAGAAG GACTCGCAGA TCAAGGGCAT CATCAACAGC
    CGATTTATGG TGCCCACTCT GGAACATGAT TGGCCCCTGC AGGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159374.1
    CDS176..4063
    Translation

    Target ORF information:

    RefSeq Version XM_005159317.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159317.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCCTATG TGTCTTCACA AGATGGAAGG TGTGTGTCTC CATGTTCTCG GCCTCACACT 
    CCTCTGAATG GCCACGCACA CGACTCCCCC GGCTGGAGCC GACAGCTCCG CGTCAGAGAT
    GGAGACCAGT TCTACCTGCT GGATCAAGAG GAGGTTCATC CACTGCTGAT GAATGATCAT
    CGTCCCGTCT CTCACCGACA CAAACTGCTG CGCAGGACCG TCAGCGTACC TGCAGATAGC
    AGACCCCACC CTGAGTCAGA GCACGCTCGT GCTCGCAGGA AGAGCTTTGC CACGTCGCGG
    CAGAGAAGCA TGGAAACTCC GCCCACTGCC CCTACACAGC CATTTCGACA GCCTAGTTTT
    CTCAGCCGCC GCTTGAAAGG ATCAATTAAA CGAGCAAAGA GTCAACCCAA ACTCGACCGC
    ACGGGAAGCT TCCGCCACAT GATTCTCCCA CGATTCCGCA GTGCTGACCA GGAGAGGACG
    CGTCTGATGC AGAGCTTTAA GGAGTCTCAC TCTCATGAGT CTCTGCTGTC TCCGAGCAGC
    GCGGCCGAGG CTCTGGACCT CACGCTGGAC GAGGACGCCA TCATCAAACC CGTCCACTCC
    AGCATCCTCG GCCAGGAGTA CTGCTTCGAG GTGACCACGG CGTCAGGAAC CAAGTGTTTC
    GCCTGCCGCT CCGCTGCTGA GAGAGACAAA TGGATCGAGA ATCTGCAGCG AGCCGTCAAA
    CCAAACAAGG ACAACAGTCG TCGTGTGGAT AACGTGCTGA AGTTATGGAT CATCGAGGCT
    CGAGATCTGC CACCGAAGAA GCGCTACTAC TGCGAGCTGT GTCTGGACGA CATGCTGTAC
    GCTCGAACCA CCAGCAAACC GCGCACCGAC ACCGTCTTCT GGGGCGAACA CTTCGAGTTC
    AACAACCTGC CGGCCATCCG CAGCCTCCGA CTGCACCTCT ACAAGGAGAA CGACAAGAAG
    AGGAGGAAGG AGAAGAGCAC GTATCTGGGT CTGGTCAGCA TCCCGATCCC GAGCGTGACG
    GGTCGTCAGT TTGTGGAGCA GTGGTACCCG CTCATCCAGC CCAGTGTTCT GGCTAAAGGC
    GGCGGCGGCG GGGGGAAGAT CATCAACGCC TCGCTGCGCC TCAAGTCCCG CTTCCAGAGC
    ATGAACATCC TGCCCATGGA GCTCTACAAG GAGTTCGCCG AGTACGTCAC CAACAACTAC
    CGCACGCTCT GCGGAGTGCT GGAGCCGCTG CTCAGCGCCA AGAGCAAAGA GGAGGTGGCC
    TGCGCGCTAG TGCACATCCT GCAGAGCACT GGGAAGGCCA AGGACTTCCT GTCTGACATG
    GCCATGTGTG AGGTGGATCG TTTCATGGAC CGTGAGCATC TGATCTTCAG AGAAAACACT
    CTCGCCACTA AAGCCATCGA GGAGTTCCTG AAGCTCATCG CACACCGCTA CCTGAAGGAC
    ACCATCGGTG AGGGAGAGTT CATCCGTGCG CTGTACGAGT CTGAGGAGAA CTGTGAGGTG
    GACCCGATGC GAACGCCTCC GTCAGTGCTG GCCGATCATC AGGCTAACCT GCGCATGTGC
    TGTGAGCTAG CGCTCTGCAA GATCATCAAC TCTCACTGCG TGTTTCCCCG TGAGCTGAAG
    GAGGTGTTTG CGTCGTGGCG TGTGCGCTGT GCTGAGCGCG GCCGAGAGGA CATCGCCGAC
    CGCCTCATCA GCTCCTCGCT CTTCCTGCGC TTCCTGTGTC CGGCGATCAT GTCCCCGTCC
    CTCTTCAACC TGACGCAGGA GTATCCCTCC GAGCGCACCT CTCGAACCCT CACGCTCATC
    GCCAAGGTGG TGCAGAGCCT GGCCAGCTTT TCAAAGTTCA GTGGGAAGGA GGACTATCTG
    GGCTTCATGA ATGAGTTTCT GGAGATGGAG TGGGGCTCCA TGCAGCAGTT CCTCTACGAG
    ATCTCCAATC TGGAGTGTGT GAGTAACGCC GGGGCGTTCG AGGGCTACAT CGACCTGGGC
    CGCGAGCTCT CCATCCTGCA CAGTCTGCTG TGGGAGGTCA TGGCCCAGCT CAGCAAGGAT
    GCGATCCTGA AGCTGGGTCC TCTGCCGCGG CTGCTGAATG ACATCAGTGT GGCCCTGCGT
    AACCCTCAGC TGCACAGACA GGCCAGCCAC CAGCCACCCG AGCGACACCT GAGCCGGCCT
    GCGTTCAGCA GACTCGCCAC CAATGACTTC CAGAGCCTCA TGATGAGAGA CCTCAACAGC
    TCCATAGACA TCTCTCGACT GCCGTCTCCT ACAGCCGGTC TGTCCGGCAG CGGTGTTCTC
    TCTCACCCGG GCATGGCTGC GTTCTCAGAG CGAGATCCCC GCGGCAACAA AGACGTCTTC
    TATGTGACCC GCCCACCTCT CGCACGCTCA AGCCCCGCCT ACTGCACCAG CAGCTCTGAC
    ATCACTGAGC CAGACCCCAA GGTACTCAGC GTCAATAAGA GTGTGTCTAT GATGGACCTG
    CAGGACTCGC GCATCAACAG CGTGTCTAAC CTGCACTCAG TGAATGATCT GCTGAACTCG
    TCTCAGGGTT CTCTGGCTGG GTTCGGTCCG CTCGGCGGTG CCCCCCTCAG GGCTGGAGGA
    CGCGTGTCTG CGGGCTCGGG CGGCTCCAGC ATGTCTGGCG GTCTGCGTTT GACCACAGAC
    TCTCTGTCGC AGCAGCAACA GCAGGCCGCC GCCGCCATGC ACGTGCCGCT GTCCTTCCAG
    AACCCTCTGT TCCACCTAGC ATCAGATGGG CCGCAGTATC ACACACCTCC ACACGGGCCC
    ACTGCTGTAC CGCCACCGCT GCTGCTGGCG CCCGAGCCAG AGAACGGGCA TGTGGGTTTC
    GGCGTTCCCA CATTCGCCCA CAGTGGGTTC TCCCGCAGTG AAGATCTGTC AGCGCTGCGG
    ACGCAGAACA GTCTGGTGCA GCCCAACATC GTGCACTCGC ACAGCTACAG CGACGACTTC
    ACACGACACA ACCAGGCCGA CTACGCCCGC AGACAGCTGT CTCTGCAGGT GCAGGAGAAT
    CTCCAGCAGC AGGTGCTGAT GGGAATGACC CCGCAGACGG CCACAGGCAC CGGCACTCCT
    GCTTCTCTGG CCACACCTCC CACCACCATT CATCCTGTCC GTCAGGCCTC CATGGCTCCG
    CCCCCTCCTC AACGCGCGAA GCCTCAGCCC GCCCACCAGC TGTCCGTCAG CTCGGCTGTA
    AACTCCACCC CCCCTAAGAC CCGCCCACAG AGCGGCAACC TCCTCCAATC GCCTGAGTCT
    AGTTTTGGAG GGAGGCAGTT GACAAGGCAG CAGCTGTCAG TCAAGGACAG CCCCGCCCCC
    GGGTTGCCGC ATCAGTCCAG CAATGCGTCG GAGAATCCCG CTCCTCAGAG CGCAGCCAAT
    GAGAGCACAG AGAATACGCC GACCAACCAA TCAGCAAAAA ACACACAGCA GAGCAACCAA
    CAACACCTGC TCAAGCCATC CGGGAACAAA CAGGGCTCTA AGTCTCCCTC CACCCTGAAC
    CCCACCTCTG CAGCCTCCGA GCGCACCGTG GCCTGGGTCT CCAACATGCC ACATCTGTCT
    GCAGACATCG AGAGTCTGCG CGTGGACAGA GAGGAGTTCA AACTCAAGGA GCACTCCAAG
    AGCATGGATG AGTCCCGCCT GGACCGGGTG CGTGAGTACG AGGAGGAGAT CAACTCCCTG
    AAGGAGCGTC TGATGATGTC CCACCGTAAG CTGGAGGAGT ACGAGCGACG GCTACACTCG
    CAGGAGCAGC AGACCAGCAA GATTCTGCTG CAGTACCAGA GCCGGCTGGA CGACAGCGAG
    AGGCGCCTGC GGCAGCAGCA GGTGGAGAAG GACTCGCAGA TCAAGGGCAT CATCAACAGC
    CGATTTATGG TGCCCACTCT GGAACATGAT TGGCCCCTGC AGGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23696
    Clone ID Related Accession (Same CDS sequence) XM_021468219.1 , XM_005159318.3 , XM_009293917.3 , XM_009293917.2 , XM_005159318.4
    Accession Version XM_021468219.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras/Rap GTPase-activating protein SynGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAACTC CGCCCACTGC CCCTACACAG CCATTTCGAC AGCCTAGTTT TCTCAGCCGC 
    CGCTTGAAAG GATCAATTAA ACGAGCAAAG AGTCAACCCA AACTCGACCG CACGGGAAGC
    TTCCGCCACA TGATTCTCCC ACGATTCCGC AGTGCTGACC AGGAGAGGAC GCGTCTGATG
    CAGAGCTTTA AGGAGTCTCA CTCTCATGAG TCTCTGCTGT CTCCGAGCAG CGCGGCCGAG
    GCTCTGGACC TCACGCTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTC
    GGCCAGGAGT ACTGCTTCGA GGTGACCACG GCGTCAGGAA CCAAGTGTTT CGCCTGCCGC
    TCCGCTGCTG AGAGAGACAA ATGGATCGAG AATCTGCAGC GAGCCGTCAA ACCAAACAAG
    GACAACAGTC GTCGTGTGGA TAACGTGCTG AAGTTATGGA TCATCGAGGC TCGAGATCTG
    CCACCGAAGA AGCGCTACTA CTGCGAGCTG TGTCTGGACG ACATGCTGTA CGCTCGAACC
    ACCAGCAAAC CGCGCACCGA CACCGTCTTC TGGGGCGAAC ACTTCGAGTT CAACAACCTG
    CCGGCCATCC GCAGCCTCCG ACTGCACCTC TACAAGGAGA ACGACAAGAA GAGGAGGAAG
    GAGAAGAGCA CGTATCTGGG TCTGGTCAGC ATCCCGATCC CGAGCGTGAC GGGTCGTCAG
    TTTGTGGAGC AGTGGTACCC GCTCATCCAG CCCAGTGTTC TGGCTAAAGG CGGCGGCGGC
    GGGGGGAAGA TCATCAACGC CTCGCTGCGC CTCAAGTCCC GCTTCCAGAG CATGAACATC
    CTGCCCATGG AGCTCTACAA GGAGTTCGCC GAGTACGTCA CCAACAACTA CCGCACGCTC
    TGCGGAGTGC TGGAGCCGCT GCTCAGCGCC AAGAGCAAAG AGGAGGTGGC CTGCGCGCTA
    GTGCACATCC TGCAGAGCAC TGGGAAGGCC AAGGACTTCC TGTCTGACAT GGCCATGTGT
    GAGGTGGATC GTTTCATGGA CCGTGAGCAT CTGATCTTCA GAGAAAACAC TCTCGCCACT
    AAAGCCATCG AGGAGTTCCT GAAGCTCATC GCACACCGCT ACCTGAAGGA CACCATCGGT
    GAGGGAGAGT TCATCCGTGC GCTGTACGAG TCTGAGGAGA ACTGTGAGGT GGACCCGATG
    CGAACGCCTC CGTCAGTGCT GGCCGATCAT CAGGCTAACC TGCGCATGTG CTGTGAGCTA
    GCGCTCTGCA AGATCATCAA CTCTCACTGC GTGTTTCCCC GTGAGCTGAA GGAGGTGTTT
    GCGTCGTGGC GTGTGCGCTG TGCTGAGCGC GGCCGAGAGG ACATCGCCGA CCGCCTCATC
    AGCTCCTCGC TCTTCCTGCG CTTCCTGTGT CCGGCGATCA TGTCCCCGTC CCTCTTCAAC
    CTGACGCAGG AGTATCCCTC CGAGCGCACC TCTCGAACCC TCACGCTCAT CGCCAAGGTG
    GTGCAGAGCC TGGCCAGCTT TTCAAAGTTC AGTGGGAAGG AGGACTATCT GGGCTTCATG
    AATGAGTTTC TGGAGATGGA GTGGGGCTCC ATGCAGCAGT TCCTCTACGA GATCTCCAAT
    CTGGAGTGTG TGAGTAACGC CGGGGCGTTC GAGGGCTACA TCGACCTGGG CCGCGAGCTC
    TCCATCCTGC ACAGTCTGCT GTGGGAGGTC ATGGCCCAGC TCAGCAAGGA TGCGATCCTG
    AAGCTGGGTC CTCTGCCGCG GCTGCTGAAT GACATCAGTG TGGCCCTGCG TAACCCTCAG
    CTGCACAGAC AGGCCAGCCA CCAGCCACCC GAGCGACACC TGAGCCGGCC TGCGTTCAGC
    AGACTCGCCA CCAATGACTT CCAGAGCCTC ATGATGAGAG ACCTCAACAG CTCCATAGAC
    ATCTCTCGAC TGCCGTCTCC TACAGCCGGT CTGTCCGGCA GCGGTGTTCT CTCTCACCCG
    GGCATGGCTG CGTTCTCAGA GCGAGATCCC CGCGGCAACA AAGACGTCTT CTATGTGACC
    CGCCCACCTC TCGCACGCTC AAGCCCCGCC TACTGCACCA GCAGCTCTGA CATCACTGAG
    CCAGACCCCA AGGTACTCAG CGTCAATAAG AGTGTGTCTA TGATGGACCT GCAGGACTCG
    CGCATCAACA GCGTGTCTAA CCTGCACTCA GTGAATGATC TGCTGAACTC GTCTCAGGGT
    TCTCTGGCTG GGTTCGGTCC GCTCGGCGGT GCCCCCCTCA GGGCTGGAGG ACGCGTGTCT
    GCGGGCTCGG GCGGCTCCAG CATGTCTGGC GGTCTGCGTT TGACCACAGA CTCTCTGTCG
    CAGCAGCAAC AGCAGGCCGC CGCCGCCATG CACGTGCCGC TGTCCTTCCA GAACCCTCTG
    TTCCACCTAG CATCAGATGG GCCGCAGTAT CACACACCTC CACACGGGCC CACTGCTGTA
    CCGCCACCGC TGCTGCTGGC GCCCGAGCCA GAGAACGGGC ATGTGGGTTT CGGCGTTCCC
    ACATTCGCCC ACAGTGGGTT CTCCCGCAGT GAAGATCTGT CAGCGCTGCG GACGCAGAAC
    AGTCTGGTGC AGCCCAACAT CGTGCACTCG CACAGCTACA GCGACGACTT CACACGACAC
    AACCAGGCCG ACTACGCCCG CAGACAGCTG TCTCTGCAGG TGCAGGAGAA TCTCCAGCAG
    CAGGTGCTGA TGGGAATGAC CCCGCAGACG GCCACAGGCA CCGGCACTCC TGCTTCTCTG
    GCCACACCTC CCACCACCAT TCATCCTGTC CGTCAGGCCT CCATGGCTCC GCCCCCTCCT
    CAACGCGCGA AGCCTCAGCC CGCCCACCAG CTGTCCGTCA GCTCGGCTGT AAACTCCACC
    CCCCCTAAGA CCCGCCCACA GAGCGGCAAC CTCCTCCAAT CGCCTGAGTC TAGTTTTGGA
    GGGAGGCAGT TGACAAGGCA GCAGCTGTCA GTCAAGGACA GCCCCGCCCC CGGGTTGCCG
    CATCAGTCCA GCAATGCGTC GGAGAATCCC GCTCCTCAGA GCGCAGCCAA TGAGAGCACA
    GAGAATACGC CGACCAACCA ATCAGCAAAA AACACACAGC AGAGCAACCA ACAACACCTG
    CTCAAGCCAT CCGGGAACAA ACAGGGCTCT AAGTCTCCCT CCACCCTGAA CCCCACCTCT
    GCAGCCTCCG AGCGCACCGT GGCCTGGGTC TCCAACATGC CACATCTGTC TGCAGACATC
    GAGAGTCTGC GCGTGGACAG AGAGGAGTTC AAACTCAAGG AGCACTCCAA GAGCATGGAT
    GAGTCCCGCC TGGACCGGGT GCGTGAGTAC GAGGAGGAGA TCAACTCCCT GAAGGAGCGT
    CTGATGATGT CCCACCGTAA GCTGGAGGAG TACGAGCGAC GGCTACACTC GCAGGAGCAG
    CAGACCAGCA AGATTCTGCT GCAGTACCAG AGCCGGCTGG ACGACAGCGA GAGGCGCCTG
    CGGCAGCAGC AGGTGGAGAA GGACTCGCAG ATCAAGGGCA TCATCAACAG GTTGATGGCG
    GTAGAGGATG AGCTGAGGGG AAGTGGAATG CCTGACCTCA AAACCAGAAT ATTCACAGAA
    CAGCCGATTT ATGGTGCCCA CTCTGGAACA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323894.1
    CDS68..3700
    Translation

    Target ORF information:

    RefSeq Version XM_021468219.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468219.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAACTC CGCCCACTGC CCCTACACAG CCATTTCGAC AGCCTAGTTT TCTCAGCCGC 
    CGCTTGAAAG GATCAATTAA ACGAGCAAAG AGTCAACCCA AACTCGACCG CACGGGAAGC
    TTCCGCCACA TGATTCTCCC ACGATTCCGC AGTGCTGACC AGGAGAGGAC GCGTCTGATG
    CAGAGCTTTA AGGAGTCTCA CTCTCATGAG TCTCTGCTGT CTCCGAGCAG CGCGGCCGAG
    GCTCTGGACC TCACGCTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTC
    GGCCAGGAGT ACTGCTTCGA GGTGACCACG GCGTCAGGAA CCAAGTGTTT CGCCTGCCGC
    TCCGCTGCTG AGAGAGACAA ATGGATCGAG AATCTGCAGC GAGCCGTCAA ACCAAACAAG
    GACAACAGTC GTCGTGTGGA TAACGTGCTG AAGTTATGGA TCATCGAGGC TCGAGATCTG
    CCACCGAAGA AGCGCTACTA CTGCGAGCTG TGTCTGGACG ACATGCTGTA CGCTCGAACC
    ACCAGCAAAC CGCGCACCGA CACCGTCTTC TGGGGCGAAC ACTTCGAGTT CAACAACCTG
    CCGGCCATCC GCAGCCTCCG ACTGCACCTC TACAAGGAGA ACGACAAGAA GAGGAGGAAG
    GAGAAGAGCA CGTATCTGGG TCTGGTCAGC ATCCCGATCC CGAGCGTGAC GGGTCGTCAG
    TTTGTGGAGC AGTGGTACCC GCTCATCCAG CCCAGTGTTC TGGCTAAAGG CGGCGGCGGC
    GGGGGGAAGA TCATCAACGC CTCGCTGCGC CTCAAGTCCC GCTTCCAGAG CATGAACATC
    CTGCCCATGG AGCTCTACAA GGAGTTCGCC GAGTACGTCA CCAACAACTA CCGCACGCTC
    TGCGGAGTGC TGGAGCCGCT GCTCAGCGCC AAGAGCAAAG AGGAGGTGGC CTGCGCGCTA
    GTGCACATCC TGCAGAGCAC TGGGAAGGCC AAGGACTTCC TGTCTGACAT GGCCATGTGT
    GAGGTGGATC GTTTCATGGA CCGTGAGCAT CTGATCTTCA GAGAAAACAC TCTCGCCACT
    AAAGCCATCG AGGAGTTCCT GAAGCTCATC GCACACCGCT ACCTGAAGGA CACCATCGGT
    GAGGGAGAGT TCATCCGTGC GCTGTACGAG TCTGAGGAGA ACTGTGAGGT GGACCCGATG
    CGAACGCCTC CGTCAGTGCT GGCCGATCAT CAGGCTAACC TGCGCATGTG CTGTGAGCTA
    GCGCTCTGCA AGATCATCAA CTCTCACTGC GTGTTTCCCC GTGAGCTGAA GGAGGTGTTT
    GCGTCGTGGC GTGTGCGCTG TGCTGAGCGC GGCCGAGAGG ACATCGCCGA CCGCCTCATC
    AGCTCCTCGC TCTTCCTGCG CTTCCTGTGT CCGGCGATCA TGTCCCCGTC CCTCTTCAAC
    CTGACGCAGG AGTATCCCTC CGAGCGCACC TCTCGAACCC TCACGCTCAT CGCCAAGGTG
    GTGCAGAGCC TGGCCAGCTT TTCAAAGTTC AGTGGGAAGG AGGACTATCT GGGCTTCATG
    AATGAGTTTC TGGAGATGGA GTGGGGCTCC ATGCAGCAGT TCCTCTACGA GATCTCCAAT
    CTGGAGTGTG TGAGTAACGC CGGGGCGTTC GAGGGCTACA TCGACCTGGG CCGCGAGCTC
    TCCATCCTGC ACAGTCTGCT GTGGGAGGTC ATGGCCCAGC TCAGCAAGGA TGCGATCCTG
    AAGCTGGGTC CTCTGCCGCG GCTGCTGAAT GACATCAGTG TGGCCCTGCG TAACCCTCAG
    CTGCACAGAC AGGCCAGCCA CCAGCCACCC GAGCGACACC TGAGCCGGCC TGCGTTCAGC
    AGACTCGCCA CCAATGACTT CCAGAGCCTC ATGATGAGAG ACCTCAACAG CTCCATAGAC
    ATCTCTCGAC TGCCGTCTCC TACAGCCGGT CTGTCCGGCA GCGGTGTTCT CTCTCACCCG
    GGCATGGCTG CGTTCTCAGA GCGAGATCCC CGCGGCAACA AAGACGTCTT CTATGTGACC
    CGCCCACCTC TCGCACGCTC AAGCCCCGCC TACTGCACCA GCAGCTCTGA CATCACTGAG
    CCAGACCCCA AGGTACTCAG CGTCAATAAG AGTGTGTCTA TGATGGACCT GCAGGACTCG
    CGCATCAACA GCGTGTCTAA CCTGCACTCA GTGAATGATC TGCTGAACTC GTCTCAGGGT
    TCTCTGGCTG GGTTCGGTCC GCTCGGCGGT GCCCCCCTCA GGGCTGGAGG ACGCGTGTCT
    GCGGGCTCGG GCGGCTCCAG CATGTCTGGC GGTCTGCGTT TGACCACAGA CTCTCTGTCG
    CAGCAGCAAC AGCAGGCCGC CGCCGCCATG CACGTGCCGC TGTCCTTCCA GAACCCTCTG
    TTCCACCTAG CATCAGATGG GCCGCAGTAT CACACACCTC CACACGGGCC CACTGCTGTA
    CCGCCACCGC TGCTGCTGGC GCCCGAGCCA GAGAACGGGC ATGTGGGTTT CGGCGTTCCC
    ACATTCGCCC ACAGTGGGTT CTCCCGCAGT GAAGATCTGT CAGCGCTGCG GACGCAGAAC
    AGTCTGGTGC AGCCCAACAT CGTGCACTCG CACAGCTACA GCGACGACTT CACACGACAC
    AACCAGGCCG ACTACGCCCG CAGACAGCTG TCTCTGCAGG TGCAGGAGAA TCTCCAGCAG
    CAGGTGCTGA TGGGAATGAC CCCGCAGACG GCCACAGGCA CCGGCACTCC TGCTTCTCTG
    GCCACACCTC CCACCACCAT TCATCCTGTC CGTCAGGCCT CCATGGCTCC GCCCCCTCCT
    CAACGCGCGA AGCCTCAGCC CGCCCACCAG CTGTCCGTCA GCTCGGCTGT AAACTCCACC
    CCCCCTAAGA CCCGCCCACA GAGCGGCAAC CTCCTCCAAT CGCCTGAGTC TAGTTTTGGA
    GGGAGGCAGT TGACAAGGCA GCAGCTGTCA GTCAAGGACA GCCCCGCCCC CGGGTTGCCG
    CATCAGTCCA GCAATGCGTC GGAGAATCCC GCTCCTCAGA GCGCAGCCAA TGAGAGCACA
    GAGAATACGC CGACCAACCA ATCAGCAAAA AACACACAGC AGAGCAACCA ACAACACCTG
    CTCAAGCCAT CCGGGAACAA ACAGGGCTCT AAGTCTCCCT CCACCCTGAA CCCCACCTCT
    GCAGCCTCCG AGCGCACCGT GGCCTGGGTC TCCAACATGC CACATCTGTC TGCAGACATC
    GAGAGTCTGC GCGTGGACAG AGAGGAGTTC AAACTCAAGG AGCACTCCAA GAGCATGGAT
    GAGTCCCGCC TGGACCGGGT GCGTGAGTAC GAGGAGGAGA TCAACTCCCT GAAGGAGCGT
    CTGATGATGT CCCACCGTAA GCTGGAGGAG TACGAGCGAC GGCTACACTC GCAGGAGCAG
    CAGACCAGCA AGATTCTGCT GCAGTACCAG AGCCGGCTGG ACGACAGCGA GAGGCGCCTG
    CGGCAGCAGC AGGTGGAGAA GGACTCGCAG ATCAAGGGCA TCATCAACAG GTTGATGGCG
    GTAGAGGATG AGCTGAGGGG AAGTGGAATG CCTGACCTCA AAACCAGAAT ATTCACAGAA
    CAGCCGATTT ATGGTGCCCA CTCTGGAACA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23696
    Clone ID Related Accession (Same CDS sequence) XM_021468219.1 , XM_005159318.3 , XM_009293917.3 , XM_009293917.2 , XM_005159318.4
    Accession Version XM_005159318.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ras/Rap GTPase-activating protein SynGAP isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159318.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAACTC CGCCCACTGC CCCTACACAG CCATTTCGAC AGCCTAGTTT TCTCAGCCGC 
    CGCTTGAAAG GATCAATTAA ACGAGCAAAG AGTCAACCCA AACTCGACCG CACGGGAAGC
    TTCCGCCACA TGATTCTCCC ACGATTCCGC AGTGCTGACC AGGAGAGGAC GCGTCTGATG
    CAGAGCTTTA AGGAGTCTCA CTCTCATGAG TCTCTGCTGT CTCCGAGCAG CGCGGCCGAG
    GCTCTGGACC TCACGCTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTC
    GGCCAGGAGT ACTGCTTCGA GGTGACCACG GCGTCAGGAA CCAAGTGTTT CGCCTGCCGC
    TCCGCTGCTG AGAGAGACAA ATGGATCGAG AATCTGCAGC GAGCCGTCAA ACCAAACAAG
    GACAACAGTC GTCGTGTGGA TAACGTGCTG AAGTTATGGA TCATCGAGGC TCGAGATCTG
    CCACCGAAGA AGCGCTACTA CTGCGAGCTG TGTCTGGACG ACATGCTGTA CGCTCGAACC
    ACCAGCAAAC CGCGCACCGA CACCGTCTTC TGGGGCGAAC ACTTCGAGTT CAACAACCTG
    CCGGCCATCC GCAGCCTCCG ACTGCACCTC TACAAGGAGA ACGACAAGAA GAGGAGGAAG
    GAGAAGAGCA CGTATCTGGG TCTGGTCAGC ATCCCGATCC CGAGCGTGAC GGGTCGTCAG
    TTTGTGGAGC AGTGGTACCC GCTCATCCAG CCCAGTGTTC TGGCTAAAGG CGGCGGCGGC
    GGGGGGAAGA TCATCAACGC CTCGCTGCGC CTCAAGTCCC GCTTCCAGAG CATGAACATC
    CTGCCCATGG AGCTCTACAA GGAGTTCGCC GAGTACGTCA CCAACAACTA CCGCACGCTC
    TGCGGAGTGC TGGAGCCGCT GCTCAGCGCC AAGAGCAAAG AGGAGGTGGC CTGCGCGCTA
    GTGCACATCC TGCAGAGCAC TGGGAAGGCC AAGGACTTCC TGTCTGACAT GGCCATGTGT
    GAGGTGGATC GTTTCATGGA CCGTGAGCAT CTGATCTTCA GAGAAAACAC TCTCGCCACT
    AAAGCCATCG AGGAGTTCCT GAAGCTCATC GCACACCGCT ACCTGAAGGA CACCATCGGT
    GAGGGAGAGT TCATCCGTGC GCTGTACGAG TCTGAGGAGA ACTGTGAGGT GGACCCGATG
    CGAACGCCTC CGTCAGTGCT GGCCGATCAT CAGGCTAACC TGCGCATGTG CTGTGAGCTA
    GCGCTCTGCA AGATCATCAA CTCTCACTGC GTGTTTCCCC GTGAGCTGAA GGAGGTGTTT
    GCGTCGTGGC GTGTGCGCTG TGCTGAGCGC GGCCGAGAGG ACATCGCCGA CCGCCTCATC
    AGCTCCTCGC TCTTCCTGCG CTTCCTGTGT CCGGCGATCA TGTCCCCGTC CCTCTTCAAC
    CTGACGCAGG AGTATCCCTC CGAGCGCACC TCTCGAACCC TCACGCTCAT CGCCAAGGTG
    GTGCAGAGCC TGGCCAGCTT TTCAAAGTTC AGTGGGAAGG AGGACTATCT GGGCTTCATG
    AATGAGTTTC TGGAGATGGA GTGGGGCTCC ATGCAGCAGT TCCTCTACGA GATCTCCAAT
    CTGGAGTGTG TGAGTAACGC CGGGGCGTTC GAGGGCTACA TCGACCTGGG CCGCGAGCTC
    TCCATCCTGC ACAGTCTGCT GTGGGAGGTC ATGGCCCAGC TCAGCAAGGA TGCGATCCTG
    AAGCTGGGTC CTCTGCCGCG GCTGCTGAAT GACATCAGTG TGGCCCTGCG TAACCCTCAG
    CTGCACAGAC AGGCCAGCCA CCAGCCACCC GAGCGACACC TGAGCCGGCC TGCGTTCAGC
    AGACTCGCCA CCAATGACTT CCAGAGCCTC ATGATGAGAG ACCTCAACAG CTCCATAGAC
    ATCTCTCGAC TGCCGTCTCC TACAGCCGGT CTGTCCGGCA GCGGTGTTCT CTCTCACCCG
    GGCATGGCTG CGTTCTCAGA GCGAGATCCC CGCGGCAACA AAGACGTCTT CTATGTGACC
    CGCCCACCTC TCGCACGCTC AAGCCCCGCC TACTGCACCA GCAGCTCTGA CATCACTGAG
    CCAGACCCCA AGGTACTCAG CGTCAATAAG AGTGTGTCTA TGATGGACCT GCAGGACTCG
    CGCATCAACA GCGTGTCTAA CCTGCACTCA GTGAATGATC TGCTGAACTC GTCTCAGGGT
    TCTCTGGCTG GGTTCGGTCC GCTCGGCGGT GCCCCCCTCA GGGCTGGAGG ACGCGTGTCT
    GCGGGCTCGG GCGGCTCCAG CATGTCTGGC GGTCTGCGTT TGACCACAGA CTCTCTGTCG
    CAGCAGCAAC AGCAGGCCGC CGCCGCCATG CACGTGCCGC TGTCCTTCCA GAACCCTCTG
    TTCCACCTAG CATCAGATGG GCCGCAGTAT CACACACCTC CACACGGGCC CACTGCTGTA
    CCGCCACCGC TGCTGCTGGC GCCCGAGCCA GAGAACGGGC ATGTGGGTTT CGGCGTTCCC
    ACATTCGCCC ACAGTGGGTT CTCCCGCAGT GAAGATCTGT CAGCGCTGCG GACGCAGAAC
    AGTCTGGTGC AGCCCAACAT CGTGCACTCG CACAGCTACA GCGACGACTT CACACGACAC
    AACCAGGCCG ACTACGCCCG CAGACAGCTG TCTCTGCAGG TGCAGGAGAA TCTCCAGCAG
    CAGGTGCTGA TGGGAATGAC CCCGCAGACG GCCACAGGCA CCGGCACTCC TGCTTCTCTG
    GCCACACCTC CCACCACCAT TCATCCTGTC CGTCAGGCCT CCATGGCTCC GCCCCCTCCT
    CAACGCGCGA AGCCTCAGCC CGCCCACCAG CTGTCCGTCA GCTCGGCTGT AAACTCCACC
    CCCCCTAAGA CCCGCCCACA GAGCGGCAAC CTCCTCCAAT CGCCTGAGTC TAGTTTTGGA
    GGGAGGCAGT TGACAAGGCA GCAGCTGTCA GTCAAGGACA GCCCCGCCCC CGGGTTGCCG
    CATCAGTCCA GCAATGCGTC GGAGAATCCC GCTCCTCAGA GCGCAGCCAA TGAGAGCACA
    GAGAATACGC CGACCAACCA ATCAGCAAAA AACACACAGC AGAGCAACCA ACAACACCTG
    CTCAAGCCAT CCGGGAACAA ACAGGGCTCT AAGTCTCCCT CCACCCTGAA CCCCACCTCT
    GCAGCCTCCG AGCGCACCGT GGCCTGGGTC TCCAACATGC CACATCTGTC TGCAGACATC
    GAGAGTCTGC GCGTGGACAG AGAGGAGTTC AAACTCAAGG AGCACTCCAA GAGCATGGAT
    GAGTCCCGCC TGGACCGGGT GCGTGAGTAC GAGGAGGAGA TCAACTCCCT GAAGGAGCGT
    CTGATGATGT CCCACCGTAA GCTGGAGGAG TACGAGCGAC GGCTACACTC GCAGGAGCAG
    CAGACCAGCA AGATTCTGCT GCAGTACCAG AGCCGGCTGG ACGACAGCGA GAGGCGCCTG
    CGGCAGCAGC AGGTGGAGAA GGACTCGCAG ATCAAGGGCA TCATCAACAG GTTGATGGCG
    GTAGAGGATG AGCTGAGGGG AAGTGGAATG CCTGACCTCA AAACCAGAAT ATTCACAGAA
    CAGCCGATTT ATGGTGCCCA CTCTGGAACA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159375.1
    CDS980..4612
    Translation

    Target ORF information:

    RefSeq Version XM_005159318.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159318.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAACTC CGCCCACTGC CCCTACACAG CCATTTCGAC AGCCTAGTTT TCTCAGCCGC 
    CGCTTGAAAG GATCAATTAA ACGAGCAAAG AGTCAACCCA AACTCGACCG CACGGGAAGC
    TTCCGCCACA TGATTCTCCC ACGATTCCGC AGTGCTGACC AGGAGAGGAC GCGTCTGATG
    CAGAGCTTTA AGGAGTCTCA CTCTCATGAG TCTCTGCTGT CTCCGAGCAG CGCGGCCGAG
    GCTCTGGACC TCACGCTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTC
    GGCCAGGAGT ACTGCTTCGA GGTGACCACG GCGTCAGGAA CCAAGTGTTT CGCCTGCCGC
    TCCGCTGCTG AGAGAGACAA ATGGATCGAG AATCTGCAGC GAGCCGTCAA ACCAAACAAG
    GACAACAGTC GTCGTGTGGA TAACGTGCTG AAGTTATGGA TCATCGAGGC TCGAGATCTG
    CCACCGAAGA AGCGCTACTA CTGCGAGCTG TGTCTGGACG ACATGCTGTA CGCTCGAACC
    ACCAGCAAAC CGCGCACCGA CACCGTCTTC TGGGGCGAAC ACTTCGAGTT CAACAACCTG
    CCGGCCATCC GCAGCCTCCG ACTGCACCTC TACAAGGAGA ACGACAAGAA GAGGAGGAAG
    GAGAAGAGCA CGTATCTGGG TCTGGTCAGC ATCCCGATCC CGAGCGTGAC GGGTCGTCAG
    TTTGTGGAGC AGTGGTACCC GCTCATCCAG CCCAGTGTTC TGGCTAAAGG CGGCGGCGGC
    GGGGGGAAGA TCATCAACGC CTCGCTGCGC CTCAAGTCCC GCTTCCAGAG CATGAACATC
    CTGCCCATGG AGCTCTACAA GGAGTTCGCC GAGTACGTCA CCAACAACTA CCGCACGCTC
    TGCGGAGTGC TGGAGCCGCT GCTCAGCGCC AAGAGCAAAG AGGAGGTGGC CTGCGCGCTA
    GTGCACATCC TGCAGAGCAC TGGGAAGGCC AAGGACTTCC TGTCTGACAT GGCCATGTGT
    GAGGTGGATC GTTTCATGGA CCGTGAGCAT CTGATCTTCA GAGAAAACAC TCTCGCCACT
    AAAGCCATCG AGGAGTTCCT GAAGCTCATC GCACACCGCT ACCTGAAGGA CACCATCGGT
    GAGGGAGAGT TCATCCGTGC GCTGTACGAG TCTGAGGAGA ACTGTGAGGT GGACCCGATG
    CGAACGCCTC CGTCAGTGCT GGCCGATCAT CAGGCTAACC TGCGCATGTG CTGTGAGCTA
    GCGCTCTGCA AGATCATCAA CTCTCACTGC GTGTTTCCCC GTGAGCTGAA GGAGGTGTTT
    GCGTCGTGGC GTGTGCGCTG TGCTGAGCGC GGCCGAGAGG ACATCGCCGA CCGCCTCATC
    AGCTCCTCGC TCTTCCTGCG CTTCCTGTGT CCGGCGATCA TGTCCCCGTC CCTCTTCAAC
    CTGACGCAGG AGTATCCCTC CGAGCGCACC TCTCGAACCC TCACGCTCAT CGCCAAGGTG
    GTGCAGAGCC TGGCCAGCTT TTCAAAGTTC AGTGGGAAGG AGGACTATCT GGGCTTCATG
    AATGAGTTTC TGGAGATGGA GTGGGGCTCC ATGCAGCAGT TCCTCTACGA GATCTCCAAT
    CTGGAGTGTG TGAGTAACGC CGGGGCGTTC GAGGGCTACA TCGACCTGGG CCGCGAGCTC
    TCCATCCTGC ACAGTCTGCT GTGGGAGGTC ATGGCCCAGC TCAGCAAGGA TGCGATCCTG
    AAGCTGGGTC CTCTGCCGCG GCTGCTGAAT GACATCAGTG TGGCCCTGCG TAACCCTCAG
    CTGCACAGAC AGGCCAGCCA CCAGCCACCC GAGCGACACC TGAGCCGGCC TGCGTTCAGC
    AGACTCGCCA CCAATGACTT CCAGAGCCTC ATGATGAGAG ACCTCAACAG CTCCATAGAC
    ATCTCTCGAC TGCCGTCTCC TACAGCCGGT CTGTCCGGCA GCGGTGTTCT CTCTCACCCG
    GGCATGGCTG CGTTCTCAGA GCGAGATCCC CGCGGCAACA AAGACGTCTT CTATGTGACC
    CGCCCACCTC TCGCACGCTC AAGCCCCGCC TACTGCACCA GCAGCTCTGA CATCACTGAG
    CCAGACCCCA AGGTACTCAG CGTCAATAAG AGTGTGTCTA TGATGGACCT GCAGGACTCG
    CGCATCAACA GCGTGTCTAA CCTGCACTCA GTGAATGATC TGCTGAACTC GTCTCAGGGT
    TCTCTGGCTG GGTTCGGTCC GCTCGGCGGT GCCCCCCTCA GGGCTGGAGG ACGCGTGTCT
    GCGGGCTCGG GCGGCTCCAG CATGTCTGGC GGTCTGCGTT TGACCACAGA CTCTCTGTCG
    CAGCAGCAAC AGCAGGCCGC CGCCGCCATG CACGTGCCGC TGTCCTTCCA GAACCCTCTG
    TTCCACCTAG CATCAGATGG GCCGCAGTAT CACACACCTC CACACGGGCC CACTGCTGTA
    CCGCCACCGC TGCTGCTGGC GCCCGAGCCA GAGAACGGGC ATGTGGGTTT CGGCGTTCCC
    ACATTCGCCC ACAGTGGGTT CTCCCGCAGT GAAGATCTGT CAGCGCTGCG GACGCAGAAC
    AGTCTGGTGC AGCCCAACAT CGTGCACTCG CACAGCTACA GCGACGACTT CACACGACAC
    AACCAGGCCG ACTACGCCCG CAGACAGCTG TCTCTGCAGG TGCAGGAGAA TCTCCAGCAG
    CAGGTGCTGA TGGGAATGAC CCCGCAGACG GCCACAGGCA CCGGCACTCC TGCTTCTCTG
    GCCACACCTC CCACCACCAT TCATCCTGTC CGTCAGGCCT CCATGGCTCC GCCCCCTCCT
    CAACGCGCGA AGCCTCAGCC CGCCCACCAG CTGTCCGTCA GCTCGGCTGT AAACTCCACC
    CCCCCTAAGA CCCGCCCACA GAGCGGCAAC CTCCTCCAAT CGCCTGAGTC TAGTTTTGGA
    GGGAGGCAGT TGACAAGGCA GCAGCTGTCA GTCAAGGACA GCCCCGCCCC CGGGTTGCCG
    CATCAGTCCA GCAATGCGTC GGAGAATCCC GCTCCTCAGA GCGCAGCCAA TGAGAGCACA
    GAGAATACGC CGACCAACCA ATCAGCAAAA AACACACAGC AGAGCAACCA ACAACACCTG
    CTCAAGCCAT CCGGGAACAA ACAGGGCTCT AAGTCTCCCT CCACCCTGAA CCCCACCTCT
    GCAGCCTCCG AGCGCACCGT GGCCTGGGTC TCCAACATGC CACATCTGTC TGCAGACATC
    GAGAGTCTGC GCGTGGACAG AGAGGAGTTC AAACTCAAGG AGCACTCCAA GAGCATGGAT
    GAGTCCCGCC TGGACCGGGT GCGTGAGTAC GAGGAGGAGA TCAACTCCCT GAAGGAGCGT
    CTGATGATGT CCCACCGTAA GCTGGAGGAG TACGAGCGAC GGCTACACTC GCAGGAGCAG
    CAGACCAGCA AGATTCTGCT GCAGTACCAG AGCCGGCTGG ACGACAGCGA GAGGCGCCTG
    CGGCAGCAGC AGGTGGAGAA GGACTCGCAG ATCAAGGGCA TCATCAACAG GTTGATGGCG
    GTAGAGGATG AGCTGAGGGG AAGTGGAATG CCTGACCTCA AAACCAGAAT ATTCACAGAA
    CAGCCGATTT ATGGTGCCCA CTCTGGAACA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23696
    Clone ID Related Accession (Same CDS sequence) XM_021468219.1 , XM_005159318.3 , XM_009293917.3 , XM_009293917.2 , XM_005159318.4
    Accession Version XM_009293917.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras/Rap GTPase-activating protein SynGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293917.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAACTC CGCCCACTGC CCCTACACAG CCATTTCGAC AGCCTAGTTT TCTCAGCCGC 
    CGCTTGAAAG GATCAATTAA ACGAGCAAAG AGTCAACCCA AACTCGACCG CACGGGAAGC
    TTCCGCCACA TGATTCTCCC ACGATTCCGC AGTGCTGACC AGGAGAGGAC GCGTCTGATG
    CAGAGCTTTA AGGAGTCTCA CTCTCATGAG TCTCTGCTGT CTCCGAGCAG CGCGGCCGAG
    GCTCTGGACC TCACGCTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTC
    GGCCAGGAGT ACTGCTTCGA GGTGACCACG GCGTCAGGAA CCAAGTGTTT CGCCTGCCGC
    TCCGCTGCTG AGAGAGACAA ATGGATCGAG AATCTGCAGC GAGCCGTCAA ACCAAACAAG
    GACAACAGTC GTCGTGTGGA TAACGTGCTG AAGTTATGGA TCATCGAGGC TCGAGATCTG
    CCACCGAAGA AGCGCTACTA CTGCGAGCTG TGTCTGGACG ACATGCTGTA CGCTCGAACC
    ACCAGCAAAC CGCGCACCGA CACCGTCTTC TGGGGCGAAC ACTTCGAGTT CAACAACCTG
    CCGGCCATCC GCAGCCTCCG ACTGCACCTC TACAAGGAGA ACGACAAGAA GAGGAGGAAG
    GAGAAGAGCA CGTATCTGGG TCTGGTCAGC ATCCCGATCC CGAGCGTGAC GGGTCGTCAG
    TTTGTGGAGC AGTGGTACCC GCTCATCCAG CCCAGTGTTC TGGCTAAAGG CGGCGGCGGC
    GGGGGGAAGA TCATCAACGC CTCGCTGCGC CTCAAGTCCC GCTTCCAGAG CATGAACATC
    CTGCCCATGG AGCTCTACAA GGAGTTCGCC GAGTACGTCA CCAACAACTA CCGCACGCTC
    TGCGGAGTGC TGGAGCCGCT GCTCAGCGCC AAGAGCAAAG AGGAGGTGGC CTGCGCGCTA
    GTGCACATCC TGCAGAGCAC TGGGAAGGCC AAGGACTTCC TGTCTGACAT GGCCATGTGT
    GAGGTGGATC GTTTCATGGA CCGTGAGCAT CTGATCTTCA GAGAAAACAC TCTCGCCACT
    AAAGCCATCG AGGAGTTCCT GAAGCTCATC GCACACCGCT ACCTGAAGGA CACCATCGGT
    GAGGGAGAGT TCATCCGTGC GCTGTACGAG TCTGAGGAGA ACTGTGAGGT GGACCCGATG
    CGAACGCCTC CGTCAGTGCT GGCCGATCAT CAGGCTAACC TGCGCATGTG CTGTGAGCTA
    GCGCTCTGCA AGATCATCAA CTCTCACTGC GTGTTTCCCC GTGAGCTGAA GGAGGTGTTT
    GCGTCGTGGC GTGTGCGCTG TGCTGAGCGC GGCCGAGAGG ACATCGCCGA CCGCCTCATC
    AGCTCCTCGC TCTTCCTGCG CTTCCTGTGT CCGGCGATCA TGTCCCCGTC CCTCTTCAAC
    CTGACGCAGG AGTATCCCTC CGAGCGCACC TCTCGAACCC TCACGCTCAT CGCCAAGGTG
    GTGCAGAGCC TGGCCAGCTT TTCAAAGTTC AGTGGGAAGG AGGACTATCT GGGCTTCATG
    AATGAGTTTC TGGAGATGGA GTGGGGCTCC ATGCAGCAGT TCCTCTACGA GATCTCCAAT
    CTGGAGTGTG TGAGTAACGC CGGGGCGTTC GAGGGCTACA TCGACCTGGG CCGCGAGCTC
    TCCATCCTGC ACAGTCTGCT GTGGGAGGTC ATGGCCCAGC TCAGCAAGGA TGCGATCCTG
    AAGCTGGGTC CTCTGCCGCG GCTGCTGAAT GACATCAGTG TGGCCCTGCG TAACCCTCAG
    CTGCACAGAC AGGCCAGCCA CCAGCCACCC GAGCGACACC TGAGCCGGCC TGCGTTCAGC
    AGACTCGCCA CCAATGACTT CCAGAGCCTC ATGATGAGAG ACCTCAACAG CTCCATAGAC
    ATCTCTCGAC TGCCGTCTCC TACAGCCGGT CTGTCCGGCA GCGGTGTTCT CTCTCACCCG
    GGCATGGCTG CGTTCTCAGA GCGAGATCCC CGCGGCAACA AAGACGTCTT CTATGTGACC
    CGCCCACCTC TCGCACGCTC AAGCCCCGCC TACTGCACCA GCAGCTCTGA CATCACTGAG
    CCAGACCCCA AGGTACTCAG CGTCAATAAG AGTGTGTCTA TGATGGACCT GCAGGACTCG
    CGCATCAACA GCGTGTCTAA CCTGCACTCA GTGAATGATC TGCTGAACTC GTCTCAGGGT
    TCTCTGGCTG GGTTCGGTCC GCTCGGCGGT GCCCCCCTCA GGGCTGGAGG ACGCGTGTCT
    GCGGGCTCGG GCGGCTCCAG CATGTCTGGC GGTCTGCGTT TGACCACAGA CTCTCTGTCG
    CAGCAGCAAC AGCAGGCCGC CGCCGCCATG CACGTGCCGC TGTCCTTCCA GAACCCTCTG
    TTCCACCTAG CATCAGATGG GCCGCAGTAT CACACACCTC CACACGGGCC CACTGCTGTA
    CCGCCACCGC TGCTGCTGGC GCCCGAGCCA GAGAACGGGC ATGTGGGTTT CGGCGTTCCC
    ACATTCGCCC ACAGTGGGTT CTCCCGCAGT GAAGATCTGT CAGCGCTGCG GACGCAGAAC
    AGTCTGGTGC AGCCCAACAT CGTGCACTCG CACAGCTACA GCGACGACTT CACACGACAC
    AACCAGGCCG ACTACGCCCG CAGACAGCTG TCTCTGCAGG TGCAGGAGAA TCTCCAGCAG
    CAGGTGCTGA TGGGAATGAC CCCGCAGACG GCCACAGGCA CCGGCACTCC TGCTTCTCTG
    GCCACACCTC CCACCACCAT TCATCCTGTC CGTCAGGCCT CCATGGCTCC GCCCCCTCCT
    CAACGCGCGA AGCCTCAGCC CGCCCACCAG CTGTCCGTCA GCTCGGCTGT AAACTCCACC
    CCCCCTAAGA CCCGCCCACA GAGCGGCAAC CTCCTCCAAT CGCCTGAGTC TAGTTTTGGA
    GGGAGGCAGT TGACAAGGCA GCAGCTGTCA GTCAAGGACA GCCCCGCCCC CGGGTTGCCG
    CATCAGTCCA GCAATGCGTC GGAGAATCCC GCTCCTCAGA GCGCAGCCAA TGAGAGCACA
    GAGAATACGC CGACCAACCA ATCAGCAAAA AACACACAGC AGAGCAACCA ACAACACCTG
    CTCAAGCCAT CCGGGAACAA ACAGGGCTCT AAGTCTCCCT CCACCCTGAA CCCCACCTCT
    GCAGCCTCCG AGCGCACCGT GGCCTGGGTC TCCAACATGC CACATCTGTC TGCAGACATC
    GAGAGTCTGC GCGTGGACAG AGAGGAGTTC AAACTCAAGG AGCACTCCAA GAGCATGGAT
    GAGTCCCGCC TGGACCGGGT GCGTGAGTAC GAGGAGGAGA TCAACTCCCT GAAGGAGCGT
    CTGATGATGT CCCACCGTAA GCTGGAGGAG TACGAGCGAC GGCTACACTC GCAGGAGCAG
    CAGACCAGCA AGATTCTGCT GCAGTACCAG AGCCGGCTGG ACGACAGCGA GAGGCGCCTG
    CGGCAGCAGC AGGTGGAGAA GGACTCGCAG ATCAAGGGCA TCATCAACAG GTTGATGGCG
    GTAGAGGATG AGCTGAGGGG AAGTGGAATG CCTGACCTCA AAACCAGAAT ATTCACAGAA
    CAGCCGATTT ATGGTGCCCA CTCTGGAACA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292192.1
    CDS95..3727
    Translation

    Target ORF information:

    RefSeq Version XM_009293917.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293917.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAACTC CGCCCACTGC CCCTACACAG CCATTTCGAC AGCCTAGTTT TCTCAGCCGC 
    CGCTTGAAAG GATCAATTAA ACGAGCAAAG AGTCAACCCA AACTCGACCG CACGGGAAGC
    TTCCGCCACA TGATTCTCCC ACGATTCCGC AGTGCTGACC AGGAGAGGAC GCGTCTGATG
    CAGAGCTTTA AGGAGTCTCA CTCTCATGAG TCTCTGCTGT CTCCGAGCAG CGCGGCCGAG
    GCTCTGGACC TCACGCTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTC
    GGCCAGGAGT ACTGCTTCGA GGTGACCACG GCGTCAGGAA CCAAGTGTTT CGCCTGCCGC
    TCCGCTGCTG AGAGAGACAA ATGGATCGAG AATCTGCAGC GAGCCGTCAA ACCAAACAAG
    GACAACAGTC GTCGTGTGGA TAACGTGCTG AAGTTATGGA TCATCGAGGC TCGAGATCTG
    CCACCGAAGA AGCGCTACTA CTGCGAGCTG TGTCTGGACG ACATGCTGTA CGCTCGAACC
    ACCAGCAAAC CGCGCACCGA CACCGTCTTC TGGGGCGAAC ACTTCGAGTT CAACAACCTG
    CCGGCCATCC GCAGCCTCCG ACTGCACCTC TACAAGGAGA ACGACAAGAA GAGGAGGAAG
    GAGAAGAGCA CGTATCTGGG TCTGGTCAGC ATCCCGATCC CGAGCGTGAC GGGTCGTCAG
    TTTGTGGAGC AGTGGTACCC GCTCATCCAG CCCAGTGTTC TGGCTAAAGG CGGCGGCGGC
    GGGGGGAAGA TCATCAACGC CTCGCTGCGC CTCAAGTCCC GCTTCCAGAG CATGAACATC
    CTGCCCATGG AGCTCTACAA GGAGTTCGCC GAGTACGTCA CCAACAACTA CCGCACGCTC
    TGCGGAGTGC TGGAGCCGCT GCTCAGCGCC AAGAGCAAAG AGGAGGTGGC CTGCGCGCTA
    GTGCACATCC TGCAGAGCAC TGGGAAGGCC AAGGACTTCC TGTCTGACAT GGCCATGTGT
    GAGGTGGATC GTTTCATGGA CCGTGAGCAT CTGATCTTCA GAGAAAACAC TCTCGCCACT
    AAAGCCATCG AGGAGTTCCT GAAGCTCATC GCACACCGCT ACCTGAAGGA CACCATCGGT
    GAGGGAGAGT TCATCCGTGC GCTGTACGAG TCTGAGGAGA ACTGTGAGGT GGACCCGATG
    CGAACGCCTC CGTCAGTGCT GGCCGATCAT CAGGCTAACC TGCGCATGTG CTGTGAGCTA
    GCGCTCTGCA AGATCATCAA CTCTCACTGC GTGTTTCCCC GTGAGCTGAA GGAGGTGTTT
    GCGTCGTGGC GTGTGCGCTG TGCTGAGCGC GGCCGAGAGG ACATCGCCGA CCGCCTCATC
    AGCTCCTCGC TCTTCCTGCG CTTCCTGTGT CCGGCGATCA TGTCCCCGTC CCTCTTCAAC
    CTGACGCAGG AGTATCCCTC CGAGCGCACC TCTCGAACCC TCACGCTCAT CGCCAAGGTG
    GTGCAGAGCC TGGCCAGCTT TTCAAAGTTC AGTGGGAAGG AGGACTATCT GGGCTTCATG
    AATGAGTTTC TGGAGATGGA GTGGGGCTCC ATGCAGCAGT TCCTCTACGA GATCTCCAAT
    CTGGAGTGTG TGAGTAACGC CGGGGCGTTC GAGGGCTACA TCGACCTGGG CCGCGAGCTC
    TCCATCCTGC ACAGTCTGCT GTGGGAGGTC ATGGCCCAGC TCAGCAAGGA TGCGATCCTG
    AAGCTGGGTC CTCTGCCGCG GCTGCTGAAT GACATCAGTG TGGCCCTGCG TAACCCTCAG
    CTGCACAGAC AGGCCAGCCA CCAGCCACCC GAGCGACACC TGAGCCGGCC TGCGTTCAGC
    AGACTCGCCA CCAATGACTT CCAGAGCCTC ATGATGAGAG ACCTCAACAG CTCCATAGAC
    ATCTCTCGAC TGCCGTCTCC TACAGCCGGT CTGTCCGGCA GCGGTGTTCT CTCTCACCCG
    GGCATGGCTG CGTTCTCAGA GCGAGATCCC CGCGGCAACA AAGACGTCTT CTATGTGACC
    CGCCCACCTC TCGCACGCTC AAGCCCCGCC TACTGCACCA GCAGCTCTGA CATCACTGAG
    CCAGACCCCA AGGTACTCAG CGTCAATAAG AGTGTGTCTA TGATGGACCT GCAGGACTCG
    CGCATCAACA GCGTGTCTAA CCTGCACTCA GTGAATGATC TGCTGAACTC GTCTCAGGGT
    TCTCTGGCTG GGTTCGGTCC GCTCGGCGGT GCCCCCCTCA GGGCTGGAGG ACGCGTGTCT
    GCGGGCTCGG GCGGCTCCAG CATGTCTGGC GGTCTGCGTT TGACCACAGA CTCTCTGTCG
    CAGCAGCAAC AGCAGGCCGC CGCCGCCATG CACGTGCCGC TGTCCTTCCA GAACCCTCTG
    TTCCACCTAG CATCAGATGG GCCGCAGTAT CACACACCTC CACACGGGCC CACTGCTGTA
    CCGCCACCGC TGCTGCTGGC GCCCGAGCCA GAGAACGGGC ATGTGGGTTT CGGCGTTCCC
    ACATTCGCCC ACAGTGGGTT CTCCCGCAGT GAAGATCTGT CAGCGCTGCG GACGCAGAAC
    AGTCTGGTGC AGCCCAACAT CGTGCACTCG CACAGCTACA GCGACGACTT CACACGACAC
    AACCAGGCCG ACTACGCCCG CAGACAGCTG TCTCTGCAGG TGCAGGAGAA TCTCCAGCAG
    CAGGTGCTGA TGGGAATGAC CCCGCAGACG GCCACAGGCA CCGGCACTCC TGCTTCTCTG
    GCCACACCTC CCACCACCAT TCATCCTGTC CGTCAGGCCT CCATGGCTCC GCCCCCTCCT
    CAACGCGCGA AGCCTCAGCC CGCCCACCAG CTGTCCGTCA GCTCGGCTGT AAACTCCACC
    CCCCCTAAGA CCCGCCCACA GAGCGGCAAC CTCCTCCAAT CGCCTGAGTC TAGTTTTGGA
    GGGAGGCAGT TGACAAGGCA GCAGCTGTCA GTCAAGGACA GCCCCGCCCC CGGGTTGCCG
    CATCAGTCCA GCAATGCGTC GGAGAATCCC GCTCCTCAGA GCGCAGCCAA TGAGAGCACA
    GAGAATACGC CGACCAACCA ATCAGCAAAA AACACACAGC AGAGCAACCA ACAACACCTG
    CTCAAGCCAT CCGGGAACAA ACAGGGCTCT AAGTCTCCCT CCACCCTGAA CCCCACCTCT
    GCAGCCTCCG AGCGCACCGT GGCCTGGGTC TCCAACATGC CACATCTGTC TGCAGACATC
    GAGAGTCTGC GCGTGGACAG AGAGGAGTTC AAACTCAAGG AGCACTCCAA GAGCATGGAT
    GAGTCCCGCC TGGACCGGGT GCGTGAGTAC GAGGAGGAGA TCAACTCCCT GAAGGAGCGT
    CTGATGATGT CCCACCGTAA GCTGGAGGAG TACGAGCGAC GGCTACACTC GCAGGAGCAG
    CAGACCAGCA AGATTCTGCT GCAGTACCAG AGCCGGCTGG ACGACAGCGA GAGGCGCCTG
    CGGCAGCAGC AGGTGGAGAA GGACTCGCAG ATCAAGGGCA TCATCAACAG GTTGATGGCG
    GTAGAGGATG AGCTGAGGGG AAGTGGAATG CCTGACCTCA AAACCAGAAT ATTCACAGAA
    CAGCCGATTT ATGGTGCCCA CTCTGGAACA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23696
    Clone ID Related Accession (Same CDS sequence) XM_021468219.1 , XM_005159318.3 , XM_009293917.3 , XM_009293917.2 , XM_005159318.4
    Accession Version XM_009293917.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ras/Rap GTPase-activating protein SynGAP isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293917.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAACTC CGCCCACTGC CCCTACACAG CCATTTCGAC AGCCTAGTTT TCTCAGCCGC 
    CGCTTGAAAG GATCAATTAA ACGAGCAAAG AGTCAACCCA AACTCGACCG CACGGGAAGC
    TTCCGCCACA TGATTCTCCC ACGATTCCGC AGTGCTGACC AGGAGAGGAC GCGTCTGATG
    CAGAGCTTTA AGGAGTCTCA CTCTCATGAG TCTCTGCTGT CTCCGAGCAG CGCGGCCGAG
    GCTCTGGACC TCACGCTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTC
    GGCCAGGAGT ACTGCTTCGA GGTGACCACG GCGTCAGGAA CCAAGTGTTT CGCCTGCCGC
    TCCGCTGCTG AGAGAGACAA ATGGATCGAG AATCTGCAGC GAGCCGTCAA ACCAAACAAG
    GACAACAGTC GTCGTGTGGA TAACGTGCTG AAGTTATGGA TCATCGAGGC TCGAGATCTG
    CCACCGAAGA AGCGCTACTA CTGCGAGCTG TGTCTGGACG ACATGCTGTA CGCTCGAACC
    ACCAGCAAAC CGCGCACCGA CACCGTCTTC TGGGGCGAAC ACTTCGAGTT CAACAACCTG
    CCGGCCATCC GCAGCCTCCG ACTGCACCTC TACAAGGAGA ACGACAAGAA GAGGAGGAAG
    GAGAAGAGCA CGTATCTGGG TCTGGTCAGC ATCCCGATCC CGAGCGTGAC GGGTCGTCAG
    TTTGTGGAGC AGTGGTACCC GCTCATCCAG CCCAGTGTTC TGGCTAAAGG CGGCGGCGGC
    GGGGGGAAGA TCATCAACGC CTCGCTGCGC CTCAAGTCCC GCTTCCAGAG CATGAACATC
    CTGCCCATGG AGCTCTACAA GGAGTTCGCC GAGTACGTCA CCAACAACTA CCGCACGCTC
    TGCGGAGTGC TGGAGCCGCT GCTCAGCGCC AAGAGCAAAG AGGAGGTGGC CTGCGCGCTA
    GTGCACATCC TGCAGAGCAC TGGGAAGGCC AAGGACTTCC TGTCTGACAT GGCCATGTGT
    GAGGTGGATC GTTTCATGGA CCGTGAGCAT CTGATCTTCA GAGAAAACAC TCTCGCCACT
    AAAGCCATCG AGGAGTTCCT GAAGCTCATC GCACACCGCT ACCTGAAGGA CACCATCGGT
    GAGGGAGAGT TCATCCGTGC GCTGTACGAG TCTGAGGAGA ACTGTGAGGT GGACCCGATG
    CGAACGCCTC CGTCAGTGCT GGCCGATCAT CAGGCTAACC TGCGCATGTG CTGTGAGCTA
    GCGCTCTGCA AGATCATCAA CTCTCACTGC GTGTTTCCCC GTGAGCTGAA GGAGGTGTTT
    GCGTCGTGGC GTGTGCGCTG TGCTGAGCGC GGCCGAGAGG ACATCGCCGA CCGCCTCATC
    AGCTCCTCGC TCTTCCTGCG CTTCCTGTGT CCGGCGATCA TGTCCCCGTC CCTCTTCAAC
    CTGACGCAGG AGTATCCCTC CGAGCGCACC TCTCGAACCC TCACGCTCAT CGCCAAGGTG
    GTGCAGAGCC TGGCCAGCTT TTCAAAGTTC AGTGGGAAGG AGGACTATCT GGGCTTCATG
    AATGAGTTTC TGGAGATGGA GTGGGGCTCC ATGCAGCAGT TCCTCTACGA GATCTCCAAT
    CTGGAGTGTG TGAGTAACGC CGGGGCGTTC GAGGGCTACA TCGACCTGGG CCGCGAGCTC
    TCCATCCTGC ACAGTCTGCT GTGGGAGGTC ATGGCCCAGC TCAGCAAGGA TGCGATCCTG
    AAGCTGGGTC CTCTGCCGCG GCTGCTGAAT GACATCAGTG TGGCCCTGCG TAACCCTCAG
    CTGCACAGAC AGGCCAGCCA CCAGCCACCC GAGCGACACC TGAGCCGGCC TGCGTTCAGC
    AGACTCGCCA CCAATGACTT CCAGAGCCTC ATGATGAGAG ACCTCAACAG CTCCATAGAC
    ATCTCTCGAC TGCCGTCTCC TACAGCCGGT CTGTCCGGCA GCGGTGTTCT CTCTCACCCG
    GGCATGGCTG CGTTCTCAGA GCGAGATCCC CGCGGCAACA AAGACGTCTT CTATGTGACC
    CGCCCACCTC TCGCACGCTC AAGCCCCGCC TACTGCACCA GCAGCTCTGA CATCACTGAG
    CCAGACCCCA AGGTACTCAG CGTCAATAAG AGTGTGTCTA TGATGGACCT GCAGGACTCG
    CGCATCAACA GCGTGTCTAA CCTGCACTCA GTGAATGATC TGCTGAACTC GTCTCAGGGT
    TCTCTGGCTG GGTTCGGTCC GCTCGGCGGT GCCCCCCTCA GGGCTGGAGG ACGCGTGTCT
    GCGGGCTCGG GCGGCTCCAG CATGTCTGGC GGTCTGCGTT TGACCACAGA CTCTCTGTCG
    CAGCAGCAAC AGCAGGCCGC CGCCGCCATG CACGTGCCGC TGTCCTTCCA GAACCCTCTG
    TTCCACCTAG CATCAGATGG GCCGCAGTAT CACACACCTC CACACGGGCC CACTGCTGTA
    CCGCCACCGC TGCTGCTGGC GCCCGAGCCA GAGAACGGGC ATGTGGGTTT CGGCGTTCCC
    ACATTCGCCC ACAGTGGGTT CTCCCGCAGT GAAGATCTGT CAGCGCTGCG GACGCAGAAC
    AGTCTGGTGC AGCCCAACAT CGTGCACTCG CACAGCTACA GCGACGACTT CACACGACAC
    AACCAGGCCG ACTACGCCCG CAGACAGCTG TCTCTGCAGG TGCAGGAGAA TCTCCAGCAG
    CAGGTGCTGA TGGGAATGAC CCCGCAGACG GCCACAGGCA CCGGCACTCC TGCTTCTCTG
    GCCACACCTC CCACCACCAT TCATCCTGTC CGTCAGGCCT CCATGGCTCC GCCCCCTCCT
    CAACGCGCGA AGCCTCAGCC CGCCCACCAG CTGTCCGTCA GCTCGGCTGT AAACTCCACC
    CCCCCTAAGA CCCGCCCACA GAGCGGCAAC CTCCTCCAAT CGCCTGAGTC TAGTTTTGGA
    GGGAGGCAGT TGACAAGGCA GCAGCTGTCA GTCAAGGACA GCCCCGCCCC CGGGTTGCCG
    CATCAGTCCA GCAATGCGTC GGAGAATCCC GCTCCTCAGA GCGCAGCCAA TGAGAGCACA
    GAGAATACGC CGACCAACCA ATCAGCAAAA AACACACAGC AGAGCAACCA ACAACACCTG
    CTCAAGCCAT CCGGGAACAA ACAGGGCTCT AAGTCTCCCT CCACCCTGAA CCCCACCTCT
    GCAGCCTCCG AGCGCACCGT GGCCTGGGTC TCCAACATGC CACATCTGTC TGCAGACATC
    GAGAGTCTGC GCGTGGACAG AGAGGAGTTC AAACTCAAGG AGCACTCCAA GAGCATGGAT
    GAGTCCCGCC TGGACCGGGT GCGTGAGTAC GAGGAGGAGA TCAACTCCCT GAAGGAGCGT
    CTGATGATGT CCCACCGTAA GCTGGAGGAG TACGAGCGAC GGCTACACTC GCAGGAGCAG
    CAGACCAGCA AGATTCTGCT GCAGTACCAG AGCCGGCTGG ACGACAGCGA GAGGCGCCTG
    CGGCAGCAGC AGGTGGAGAA GGACTCGCAG ATCAAGGGCA TCATCAACAG GTTGATGGCG
    GTAGAGGATG AGCTGAGGGG AAGTGGAATG CCTGACCTCA AAACCAGAAT ATTCACAGAA
    CAGCCGATTT ATGGTGCCCA CTCTGGAACA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292192.1
    CDS219..3851
    Translation

    Target ORF information:

    RefSeq Version XM_009293917.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293917.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAACTC CGCCCACTGC CCCTACACAG CCATTTCGAC AGCCTAGTTT TCTCAGCCGC 
    CGCTTGAAAG GATCAATTAA ACGAGCAAAG AGTCAACCCA AACTCGACCG CACGGGAAGC
    TTCCGCCACA TGATTCTCCC ACGATTCCGC AGTGCTGACC AGGAGAGGAC GCGTCTGATG
    CAGAGCTTTA AGGAGTCTCA CTCTCATGAG TCTCTGCTGT CTCCGAGCAG CGCGGCCGAG
    GCTCTGGACC TCACGCTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTC
    GGCCAGGAGT ACTGCTTCGA GGTGACCACG GCGTCAGGAA CCAAGTGTTT CGCCTGCCGC
    TCCGCTGCTG AGAGAGACAA ATGGATCGAG AATCTGCAGC GAGCCGTCAA ACCAAACAAG
    GACAACAGTC GTCGTGTGGA TAACGTGCTG AAGTTATGGA TCATCGAGGC TCGAGATCTG
    CCACCGAAGA AGCGCTACTA CTGCGAGCTG TGTCTGGACG ACATGCTGTA CGCTCGAACC
    ACCAGCAAAC CGCGCACCGA CACCGTCTTC TGGGGCGAAC ACTTCGAGTT CAACAACCTG
    CCGGCCATCC GCAGCCTCCG ACTGCACCTC TACAAGGAGA ACGACAAGAA GAGGAGGAAG
    GAGAAGAGCA CGTATCTGGG TCTGGTCAGC ATCCCGATCC CGAGCGTGAC GGGTCGTCAG
    TTTGTGGAGC AGTGGTACCC GCTCATCCAG CCCAGTGTTC TGGCTAAAGG CGGCGGCGGC
    GGGGGGAAGA TCATCAACGC CTCGCTGCGC CTCAAGTCCC GCTTCCAGAG CATGAACATC
    CTGCCCATGG AGCTCTACAA GGAGTTCGCC GAGTACGTCA CCAACAACTA CCGCACGCTC
    TGCGGAGTGC TGGAGCCGCT GCTCAGCGCC AAGAGCAAAG AGGAGGTGGC CTGCGCGCTA
    GTGCACATCC TGCAGAGCAC TGGGAAGGCC AAGGACTTCC TGTCTGACAT GGCCATGTGT
    GAGGTGGATC GTTTCATGGA CCGTGAGCAT CTGATCTTCA GAGAAAACAC TCTCGCCACT
    AAAGCCATCG AGGAGTTCCT GAAGCTCATC GCACACCGCT ACCTGAAGGA CACCATCGGT
    GAGGGAGAGT TCATCCGTGC GCTGTACGAG TCTGAGGAGA ACTGTGAGGT GGACCCGATG
    CGAACGCCTC CGTCAGTGCT GGCCGATCAT CAGGCTAACC TGCGCATGTG CTGTGAGCTA
    GCGCTCTGCA AGATCATCAA CTCTCACTGC GTGTTTCCCC GTGAGCTGAA GGAGGTGTTT
    GCGTCGTGGC GTGTGCGCTG TGCTGAGCGC GGCCGAGAGG ACATCGCCGA CCGCCTCATC
    AGCTCCTCGC TCTTCCTGCG CTTCCTGTGT CCGGCGATCA TGTCCCCGTC CCTCTTCAAC
    CTGACGCAGG AGTATCCCTC CGAGCGCACC TCTCGAACCC TCACGCTCAT CGCCAAGGTG
    GTGCAGAGCC TGGCCAGCTT TTCAAAGTTC AGTGGGAAGG AGGACTATCT GGGCTTCATG
    AATGAGTTTC TGGAGATGGA GTGGGGCTCC ATGCAGCAGT TCCTCTACGA GATCTCCAAT
    CTGGAGTGTG TGAGTAACGC CGGGGCGTTC GAGGGCTACA TCGACCTGGG CCGCGAGCTC
    TCCATCCTGC ACAGTCTGCT GTGGGAGGTC ATGGCCCAGC TCAGCAAGGA TGCGATCCTG
    AAGCTGGGTC CTCTGCCGCG GCTGCTGAAT GACATCAGTG TGGCCCTGCG TAACCCTCAG
    CTGCACAGAC AGGCCAGCCA CCAGCCACCC GAGCGACACC TGAGCCGGCC TGCGTTCAGC
    AGACTCGCCA CCAATGACTT CCAGAGCCTC ATGATGAGAG ACCTCAACAG CTCCATAGAC
    ATCTCTCGAC TGCCGTCTCC TACAGCCGGT CTGTCCGGCA GCGGTGTTCT CTCTCACCCG
    GGCATGGCTG CGTTCTCAGA GCGAGATCCC CGCGGCAACA AAGACGTCTT CTATGTGACC
    CGCCCACCTC TCGCACGCTC AAGCCCCGCC TACTGCACCA GCAGCTCTGA CATCACTGAG
    CCAGACCCCA AGGTACTCAG CGTCAATAAG AGTGTGTCTA TGATGGACCT GCAGGACTCG
    CGCATCAACA GCGTGTCTAA CCTGCACTCA GTGAATGATC TGCTGAACTC GTCTCAGGGT
    TCTCTGGCTG GGTTCGGTCC GCTCGGCGGT GCCCCCCTCA GGGCTGGAGG ACGCGTGTCT
    GCGGGCTCGG GCGGCTCCAG CATGTCTGGC GGTCTGCGTT TGACCACAGA CTCTCTGTCG
    CAGCAGCAAC AGCAGGCCGC CGCCGCCATG CACGTGCCGC TGTCCTTCCA GAACCCTCTG
    TTCCACCTAG CATCAGATGG GCCGCAGTAT CACACACCTC CACACGGGCC CACTGCTGTA
    CCGCCACCGC TGCTGCTGGC GCCCGAGCCA GAGAACGGGC ATGTGGGTTT CGGCGTTCCC
    ACATTCGCCC ACAGTGGGTT CTCCCGCAGT GAAGATCTGT CAGCGCTGCG GACGCAGAAC
    AGTCTGGTGC AGCCCAACAT CGTGCACTCG CACAGCTACA GCGACGACTT CACACGACAC
    AACCAGGCCG ACTACGCCCG CAGACAGCTG TCTCTGCAGG TGCAGGAGAA TCTCCAGCAG
    CAGGTGCTGA TGGGAATGAC CCCGCAGACG GCCACAGGCA CCGGCACTCC TGCTTCTCTG
    GCCACACCTC CCACCACCAT TCATCCTGTC CGTCAGGCCT CCATGGCTCC GCCCCCTCCT
    CAACGCGCGA AGCCTCAGCC CGCCCACCAG CTGTCCGTCA GCTCGGCTGT AAACTCCACC
    CCCCCTAAGA CCCGCCCACA GAGCGGCAAC CTCCTCCAAT CGCCTGAGTC TAGTTTTGGA
    GGGAGGCAGT TGACAAGGCA GCAGCTGTCA GTCAAGGACA GCCCCGCCCC CGGGTTGCCG
    CATCAGTCCA GCAATGCGTC GGAGAATCCC GCTCCTCAGA GCGCAGCCAA TGAGAGCACA
    GAGAATACGC CGACCAACCA ATCAGCAAAA AACACACAGC AGAGCAACCA ACAACACCTG
    CTCAAGCCAT CCGGGAACAA ACAGGGCTCT AAGTCTCCCT CCACCCTGAA CCCCACCTCT
    GCAGCCTCCG AGCGCACCGT GGCCTGGGTC TCCAACATGC CACATCTGTC TGCAGACATC
    GAGAGTCTGC GCGTGGACAG AGAGGAGTTC AAACTCAAGG AGCACTCCAA GAGCATGGAT
    GAGTCCCGCC TGGACCGGGT GCGTGAGTAC GAGGAGGAGA TCAACTCCCT GAAGGAGCGT
    CTGATGATGT CCCACCGTAA GCTGGAGGAG TACGAGCGAC GGCTACACTC GCAGGAGCAG
    CAGACCAGCA AGATTCTGCT GCAGTACCAG AGCCGGCTGG ACGACAGCGA GAGGCGCCTG
    CGGCAGCAGC AGGTGGAGAA GGACTCGCAG ATCAAGGGCA TCATCAACAG GTTGATGGCG
    GTAGAGGATG AGCTGAGGGG AAGTGGAATG CCTGACCTCA AAACCAGAAT ATTCACAGAA
    CAGCCGATTT ATGGTGCCCA CTCTGGAACA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23696
    Clone ID Related Accession (Same CDS sequence) XM_021468219.1 , XM_005159318.3 , XM_009293917.3 , XM_009293917.2 , XM_005159318.4
    Accession Version XM_005159318.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras/Rap GTPase-activating protein SynGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159318.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAACTC CGCCCACTGC CCCTACACAG CCATTTCGAC AGCCTAGTTT TCTCAGCCGC 
    CGCTTGAAAG GATCAATTAA ACGAGCAAAG AGTCAACCCA AACTCGACCG CACGGGAAGC
    TTCCGCCACA TGATTCTCCC ACGATTCCGC AGTGCTGACC AGGAGAGGAC GCGTCTGATG
    CAGAGCTTTA AGGAGTCTCA CTCTCATGAG TCTCTGCTGT CTCCGAGCAG CGCGGCCGAG
    GCTCTGGACC TCACGCTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTC
    GGCCAGGAGT ACTGCTTCGA GGTGACCACG GCGTCAGGAA CCAAGTGTTT CGCCTGCCGC
    TCCGCTGCTG AGAGAGACAA ATGGATCGAG AATCTGCAGC GAGCCGTCAA ACCAAACAAG
    GACAACAGTC GTCGTGTGGA TAACGTGCTG AAGTTATGGA TCATCGAGGC TCGAGATCTG
    CCACCGAAGA AGCGCTACTA CTGCGAGCTG TGTCTGGACG ACATGCTGTA CGCTCGAACC
    ACCAGCAAAC CGCGCACCGA CACCGTCTTC TGGGGCGAAC ACTTCGAGTT CAACAACCTG
    CCGGCCATCC GCAGCCTCCG ACTGCACCTC TACAAGGAGA ACGACAAGAA GAGGAGGAAG
    GAGAAGAGCA CGTATCTGGG TCTGGTCAGC ATCCCGATCC CGAGCGTGAC GGGTCGTCAG
    TTTGTGGAGC AGTGGTACCC GCTCATCCAG CCCAGTGTTC TGGCTAAAGG CGGCGGCGGC
    GGGGGGAAGA TCATCAACGC CTCGCTGCGC CTCAAGTCCC GCTTCCAGAG CATGAACATC
    CTGCCCATGG AGCTCTACAA GGAGTTCGCC GAGTACGTCA CCAACAACTA CCGCACGCTC
    TGCGGAGTGC TGGAGCCGCT GCTCAGCGCC AAGAGCAAAG AGGAGGTGGC CTGCGCGCTA
    GTGCACATCC TGCAGAGCAC TGGGAAGGCC AAGGACTTCC TGTCTGACAT GGCCATGTGT
    GAGGTGGATC GTTTCATGGA CCGTGAGCAT CTGATCTTCA GAGAAAACAC TCTCGCCACT
    AAAGCCATCG AGGAGTTCCT GAAGCTCATC GCACACCGCT ACCTGAAGGA CACCATCGGT
    GAGGGAGAGT TCATCCGTGC GCTGTACGAG TCTGAGGAGA ACTGTGAGGT GGACCCGATG
    CGAACGCCTC CGTCAGTGCT GGCCGATCAT CAGGCTAACC TGCGCATGTG CTGTGAGCTA
    GCGCTCTGCA AGATCATCAA CTCTCACTGC GTGTTTCCCC GTGAGCTGAA GGAGGTGTTT
    GCGTCGTGGC GTGTGCGCTG TGCTGAGCGC GGCCGAGAGG ACATCGCCGA CCGCCTCATC
    AGCTCCTCGC TCTTCCTGCG CTTCCTGTGT CCGGCGATCA TGTCCCCGTC CCTCTTCAAC
    CTGACGCAGG AGTATCCCTC CGAGCGCACC TCTCGAACCC TCACGCTCAT CGCCAAGGTG
    GTGCAGAGCC TGGCCAGCTT TTCAAAGTTC AGTGGGAAGG AGGACTATCT GGGCTTCATG
    AATGAGTTTC TGGAGATGGA GTGGGGCTCC ATGCAGCAGT TCCTCTACGA GATCTCCAAT
    CTGGAGTGTG TGAGTAACGC CGGGGCGTTC GAGGGCTACA TCGACCTGGG CCGCGAGCTC
    TCCATCCTGC ACAGTCTGCT GTGGGAGGTC ATGGCCCAGC TCAGCAAGGA TGCGATCCTG
    AAGCTGGGTC CTCTGCCGCG GCTGCTGAAT GACATCAGTG TGGCCCTGCG TAACCCTCAG
    CTGCACAGAC AGGCCAGCCA CCAGCCACCC GAGCGACACC TGAGCCGGCC TGCGTTCAGC
    AGACTCGCCA CCAATGACTT CCAGAGCCTC ATGATGAGAG ACCTCAACAG CTCCATAGAC
    ATCTCTCGAC TGCCGTCTCC TACAGCCGGT CTGTCCGGCA GCGGTGTTCT CTCTCACCCG
    GGCATGGCTG CGTTCTCAGA GCGAGATCCC CGCGGCAACA AAGACGTCTT CTATGTGACC
    CGCCCACCTC TCGCACGCTC AAGCCCCGCC TACTGCACCA GCAGCTCTGA CATCACTGAG
    CCAGACCCCA AGGTACTCAG CGTCAATAAG AGTGTGTCTA TGATGGACCT GCAGGACTCG
    CGCATCAACA GCGTGTCTAA CCTGCACTCA GTGAATGATC TGCTGAACTC GTCTCAGGGT
    TCTCTGGCTG GGTTCGGTCC GCTCGGCGGT GCCCCCCTCA GGGCTGGAGG ACGCGTGTCT
    GCGGGCTCGG GCGGCTCCAG CATGTCTGGC GGTCTGCGTT TGACCACAGA CTCTCTGTCG
    CAGCAGCAAC AGCAGGCCGC CGCCGCCATG CACGTGCCGC TGTCCTTCCA GAACCCTCTG
    TTCCACCTAG CATCAGATGG GCCGCAGTAT CACACACCTC CACACGGGCC CACTGCTGTA
    CCGCCACCGC TGCTGCTGGC GCCCGAGCCA GAGAACGGGC ATGTGGGTTT CGGCGTTCCC
    ACATTCGCCC ACAGTGGGTT CTCCCGCAGT GAAGATCTGT CAGCGCTGCG GACGCAGAAC
    AGTCTGGTGC AGCCCAACAT CGTGCACTCG CACAGCTACA GCGACGACTT CACACGACAC
    AACCAGGCCG ACTACGCCCG CAGACAGCTG TCTCTGCAGG TGCAGGAGAA TCTCCAGCAG
    CAGGTGCTGA TGGGAATGAC CCCGCAGACG GCCACAGGCA CCGGCACTCC TGCTTCTCTG
    GCCACACCTC CCACCACCAT TCATCCTGTC CGTCAGGCCT CCATGGCTCC GCCCCCTCCT
    CAACGCGCGA AGCCTCAGCC CGCCCACCAG CTGTCCGTCA GCTCGGCTGT AAACTCCACC
    CCCCCTAAGA CCCGCCCACA GAGCGGCAAC CTCCTCCAAT CGCCTGAGTC TAGTTTTGGA
    GGGAGGCAGT TGACAAGGCA GCAGCTGTCA GTCAAGGACA GCCCCGCCCC CGGGTTGCCG
    CATCAGTCCA GCAATGCGTC GGAGAATCCC GCTCCTCAGA GCGCAGCCAA TGAGAGCACA
    GAGAATACGC CGACCAACCA ATCAGCAAAA AACACACAGC AGAGCAACCA ACAACACCTG
    CTCAAGCCAT CCGGGAACAA ACAGGGCTCT AAGTCTCCCT CCACCCTGAA CCCCACCTCT
    GCAGCCTCCG AGCGCACCGT GGCCTGGGTC TCCAACATGC CACATCTGTC TGCAGACATC
    GAGAGTCTGC GCGTGGACAG AGAGGAGTTC AAACTCAAGG AGCACTCCAA GAGCATGGAT
    GAGTCCCGCC TGGACCGGGT GCGTGAGTAC GAGGAGGAGA TCAACTCCCT GAAGGAGCGT
    CTGATGATGT CCCACCGTAA GCTGGAGGAG TACGAGCGAC GGCTACACTC GCAGGAGCAG
    CAGACCAGCA AGATTCTGCT GCAGTACCAG AGCCGGCTGG ACGACAGCGA GAGGCGCCTG
    CGGCAGCAGC AGGTGGAGAA GGACTCGCAG ATCAAGGGCA TCATCAACAG GTTGATGGCG
    GTAGAGGATG AGCTGAGGGG AAGTGGAATG CCTGACCTCA AAACCAGAAT ATTCACAGAA
    CAGCCGATTT ATGGTGCCCA CTCTGGAACA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159375.1
    CDS1000..4632
    Translation

    Target ORF information:

    RefSeq Version XM_005159318.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio synaptic Ras GTPase activating protein 1a (syngap1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159318.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAACTC CGCCCACTGC CCCTACACAG CCATTTCGAC AGCCTAGTTT TCTCAGCCGC 
    CGCTTGAAAG GATCAATTAA ACGAGCAAAG AGTCAACCCA AACTCGACCG CACGGGAAGC
    TTCCGCCACA TGATTCTCCC ACGATTCCGC AGTGCTGACC AGGAGAGGAC GCGTCTGATG
    CAGAGCTTTA AGGAGTCTCA CTCTCATGAG TCTCTGCTGT CTCCGAGCAG CGCGGCCGAG
    GCTCTGGACC TCACGCTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTC
    GGCCAGGAGT ACTGCTTCGA GGTGACCACG GCGTCAGGAA CCAAGTGTTT CGCCTGCCGC
    TCCGCTGCTG AGAGAGACAA ATGGATCGAG AATCTGCAGC GAGCCGTCAA ACCAAACAAG
    GACAACAGTC GTCGTGTGGA TAACGTGCTG AAGTTATGGA TCATCGAGGC TCGAGATCTG
    CCACCGAAGA AGCGCTACTA CTGCGAGCTG TGTCTGGACG ACATGCTGTA CGCTCGAACC
    ACCAGCAAAC CGCGCACCGA CACCGTCTTC TGGGGCGAAC ACTTCGAGTT CAACAACCTG
    CCGGCCATCC GCAGCCTCCG ACTGCACCTC TACAAGGAGA ACGACAAGAA GAGGAGGAAG
    GAGAAGAGCA CGTATCTGGG TCTGGTCAGC ATCCCGATCC CGAGCGTGAC GGGTCGTCAG
    TTTGTGGAGC AGTGGTACCC GCTCATCCAG CCCAGTGTTC TGGCTAAAGG CGGCGGCGGC
    GGGGGGAAGA TCATCAACGC CTCGCTGCGC CTCAAGTCCC GCTTCCAGAG CATGAACATC
    CTGCCCATGG AGCTCTACAA GGAGTTCGCC GAGTACGTCA CCAACAACTA CCGCACGCTC
    TGCGGAGTGC TGGAGCCGCT GCTCAGCGCC AAGAGCAAAG AGGAGGTGGC CTGCGCGCTA
    GTGCACATCC TGCAGAGCAC TGGGAAGGCC AAGGACTTCC TGTCTGACAT GGCCATGTGT
    GAGGTGGATC GTTTCATGGA CCGTGAGCAT CTGATCTTCA GAGAAAACAC TCTCGCCACT
    AAAGCCATCG AGGAGTTCCT GAAGCTCATC GCACACCGCT ACCTGAAGGA CACCATCGGT
    GAGGGAGAGT TCATCCGTGC GCTGTACGAG TCTGAGGAGA ACTGTGAGGT GGACCCGATG
    CGAACGCCTC CGTCAGTGCT GGCCGATCAT CAGGCTAACC TGCGCATGTG CTGTGAGCTA
    GCGCTCTGCA AGATCATCAA CTCTCACTGC GTGTTTCCCC GTGAGCTGAA GGAGGTGTTT
    GCGTCGTGGC GTGTGCGCTG TGCTGAGCGC GGCCGAGAGG ACATCGCCGA CCGCCTCATC
    AGCTCCTCGC TCTTCCTGCG CTTCCTGTGT CCGGCGATCA TGTCCCCGTC CCTCTTCAAC
    CTGACGCAGG AGTATCCCTC CGAGCGCACC TCTCGAACCC TCACGCTCAT CGCCAAGGTG
    GTGCAGAGCC TGGCCAGCTT TTCAAAGTTC AGTGGGAAGG AGGACTATCT GGGCTTCATG
    AATGAGTTTC TGGAGATGGA GTGGGGCTCC ATGCAGCAGT TCCTCTACGA GATCTCCAAT
    CTGGAGTGTG TGAGTAACGC CGGGGCGTTC GAGGGCTACA TCGACCTGGG CCGCGAGCTC
    TCCATCCTGC ACAGTCTGCT GTGGGAGGTC ATGGCCCAGC TCAGCAAGGA TGCGATCCTG
    AAGCTGGGTC CTCTGCCGCG GCTGCTGAAT GACATCAGTG TGGCCCTGCG TAACCCTCAG
    CTGCACAGAC AGGCCAGCCA CCAGCCACCC GAGCGACACC TGAGCCGGCC TGCGTTCAGC
    AGACTCGCCA CCAATGACTT CCAGAGCCTC ATGATGAGAG ACCTCAACAG CTCCATAGAC
    ATCTCTCGAC TGCCGTCTCC TACAGCCGGT CTGTCCGGCA GCGGTGTTCT CTCTCACCCG
    GGCATGGCTG CGTTCTCAGA GCGAGATCCC CGCGGCAACA AAGACGTCTT CTATGTGACC
    CGCCCACCTC TCGCACGCTC AAGCCCCGCC TACTGCACCA GCAGCTCTGA CATCACTGAG
    CCAGACCCCA AGGTACTCAG CGTCAATAAG AGTGTGTCTA TGATGGACCT GCAGGACTCG
    CGCATCAACA GCGTGTCTAA CCTGCACTCA GTGAATGATC TGCTGAACTC GTCTCAGGGT
    TCTCTGGCTG GGTTCGGTCC GCTCGGCGGT GCCCCCCTCA GGGCTGGAGG ACGCGTGTCT
    GCGGGCTCGG GCGGCTCCAG CATGTCTGGC GGTCTGCGTT TGACCACAGA CTCTCTGTCG
    CAGCAGCAAC AGCAGGCCGC CGCCGCCATG CACGTGCCGC TGTCCTTCCA GAACCCTCTG
    TTCCACCTAG CATCAGATGG GCCGCAGTAT CACACACCTC CACACGGGCC CACTGCTGTA
    CCGCCACCGC TGCTGCTGGC GCCCGAGCCA GAGAACGGGC ATGTGGGTTT CGGCGTTCCC
    ACATTCGCCC ACAGTGGGTT CTCCCGCAGT GAAGATCTGT CAGCGCTGCG GACGCAGAAC
    AGTCTGGTGC AGCCCAACAT CGTGCACTCG CACAGCTACA GCGACGACTT CACACGACAC
    AACCAGGCCG ACTACGCCCG CAGACAGCTG TCTCTGCAGG TGCAGGAGAA TCTCCAGCAG
    CAGGTGCTGA TGGGAATGAC CCCGCAGACG GCCACAGGCA CCGGCACTCC TGCTTCTCTG
    GCCACACCTC CCACCACCAT TCATCCTGTC CGTCAGGCCT CCATGGCTCC GCCCCCTCCT
    CAACGCGCGA AGCCTCAGCC CGCCCACCAG CTGTCCGTCA GCTCGGCTGT AAACTCCACC
    CCCCCTAAGA CCCGCCCACA GAGCGGCAAC CTCCTCCAAT CGCCTGAGTC TAGTTTTGGA
    GGGAGGCAGT TGACAAGGCA GCAGCTGTCA GTCAAGGACA GCCCCGCCCC CGGGTTGCCG
    CATCAGTCCA GCAATGCGTC GGAGAATCCC GCTCCTCAGA GCGCAGCCAA TGAGAGCACA
    GAGAATACGC CGACCAACCA ATCAGCAAAA AACACACAGC AGAGCAACCA ACAACACCTG
    CTCAAGCCAT CCGGGAACAA ACAGGGCTCT AAGTCTCCCT CCACCCTGAA CCCCACCTCT
    GCAGCCTCCG AGCGCACCGT GGCCTGGGTC TCCAACATGC CACATCTGTC TGCAGACATC
    GAGAGTCTGC GCGTGGACAG AGAGGAGTTC AAACTCAAGG AGCACTCCAA GAGCATGGAT
    GAGTCCCGCC TGGACCGGGT GCGTGAGTAC GAGGAGGAGA TCAACTCCCT GAAGGAGCGT
    CTGATGATGT CCCACCGTAA GCTGGAGGAG TACGAGCGAC GGCTACACTC GCAGGAGCAG
    CAGACCAGCA AGATTCTGCT GCAGTACCAG AGCCGGCTGG ACGACAGCGA GAGGCGCCTG
    CGGCAGCAGC AGGTGGAGAA GGACTCGCAG ATCAAGGGCA TCATCAACAG GTTGATGGCG
    GTAGAGGATG AGCTGAGGGG AAGTGGAATG CCTGACCTCA AAACCAGAAT ATTCACAGAA
    CAGCCGATTT ATGGTGCCCA CTCTGGAACA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.