syngap1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	syngap1b
Entrez Gene ID	100151149
Full Name	synaptic Ras GTPase activating protein 1b
Synonyms	si:dkey-246f6.1
General protein information	Preferred Names synaptic Ras GTPase activating protein 1b Names ras/Rap GTPase-activating protein SynGAP1b syngap1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	This gene encodes a Ras GTPase activating protein that is a member of the N-methyl-D-aspartate receptor complex. In mammals, the N-terminal domain of the protein contains a Ras-GAP domain, a pleckstrin homology domain, and a C2 domain that may be involved in binding of calcium and phospholipids. The C-terminal domain consists of a ten histidine repeat region, serine and tyrosine phosphorylation sites, and a T/SXV motif required for postsynaptic scaffold protein interaction. In humans, allelic variants of this gene are associated with intellectual disability and autism spectrum disorder. Morpholino knockdown of this gene in zebrafish results in neural cell death, developmental delay of mid- and hindbrain regions, seizure-like behavior, and disrupted motor behaviors. [provided by RefSeq, Nov 2016].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017351306.1	XP_017206795.1	si:dkey-246f6.1 isoform X10
XM_009292645.2	XP_009290920.1	si:dkey-246f6.1 isoform X5
XM_021466578.1	XP_021322253.1	ras/Rap GTPase-activating protein SynGAP1b isoform X2
XM_021466585.1	XP_021322260.1	ras/Rap GTPase-activating protein SynGAP1b isoform X8
XM_009292648.2	XP_009290923.1	si:dkey-246f6.1 isoform X7
XM_009292649.2	XP_009290924.1	si:dkey-246f6.1 isoform X8
XM_009292642.2	XP_009290917.1	si:dkey-246f6.1 isoform X2
NM_001326467.1	NP_001313396.1	ras/Rap GTPase-activating protein SynGAP1b
XM_021466580.1	XP_021322255.1	ras/Rap GTPase-activating protein SynGAP1b isoform X4
XM_009292643.2	XP_009290918.1	si:dkey-246f6.1 isoform X3
XM_021466579.1	XP_021322254.1	ras/Rap GTPase-activating protein SynGAP1b isoform X3
XM_021466586.1	XP_021322261.1	ras/Rap GTPase-activating protein SynGAP1b isoform X9
XM_021466588.1	XP_021322263.1	ras/Rap GTPase-activating protein SynGAP1b isoform X11
XM_021466589.1	XP_021322264.1	ras/Rap GTPase-activating protein SynGAP1b isoform X12
XM_021466590.1	XP_021322265.1	ras/Rap GTPase-activating protein SynGAP1b isoform X13
XM_009292644.2	XP_009290919.1	si:dkey-246f6.1 isoform X4
XM_009292646.2	XP_009290921.1	si:dkey-246f6.1 isoform X6
XM_009292650.2	XP_009290925.1	si:dkey-246f6.1 isoform X9
XM_021466581.1	XP_021322256.1	ras/Rap GTPase-activating protein SynGAP1b isoform X5
XM_021466583.1	XP_021322258.1	ras/Rap GTPase-activating protein SynGAP1b isoform X7
XM_021466584.1	XP_021322259.1	ras/Rap GTPase-activating protein SynGAP1b isoform X8
XM_021466587.1	XP_021322262.1	ras/Rap GTPase-activating protein SynGAP1b isoform X10
XM_017351305.1	XP_017206794.1	si:dkey-246f6.1 isoform X1
XM_009292652.2	XP_009290927.1	si:dkey-246f6.1 isoform X11
XM_021466577.1	XP_021322252.1	ras/Rap GTPase-activating protein SynGAP1b isoform X1
XM_021466582.1	XP_021322257.1	ras/Rap GTPase-activating protein SynGAP1b isoform X6

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Syngap1	NP_851606.3
Danio rerio (zebrafish)	si:dkeyp-24a7.8	XP_005159373.1
Xenopus tropicalis (tropical clawed frog)	syngap1	NP_001128288.1
Homo sapiens (human)	SYNGAP1	NP_006763.2
	SYNGAP1	XP_001109824.1
Canis lupus familiaris (dog)	SYNGAP1	XP_005627810.1
Bos taurus (cattle)	SYNGAP1	NP_001192395.1
Mus musculus (house mouse)	Syngap1	NP_001268420.1
Pan troglodytes (chimpanzee)	SYNGAP1	XP_003311264.1
Danio rerio (zebrafish)	LOC100151149	XP_001919204.4

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Two knockdown models of the autism genes SYNGAP1 and SHANK3 in zebrafish produce similar behavioral phenotypes associated with embryonic disruptions of brain morphogenesis.
Kozol RA, Cukier HN, Zou B, Mayo V, De Rubeis S, Cai G, Griswold AJ, Whitehead PL, Haines JL, Gilbert JR, Cuccaro ML, Martin ER, Baker JD, Buxbaum JD, Pericak-Vance MA, Dallman JE
Human molecular genetics24(14)4006-23(2015 Jul)

Evolutionary history of the ABCB2 genomic region in teleosts.
Palti Y, Rodriguez MF, Gahr SA, Hansen JD
Developmental and comparative immunology31(5)483-98(2007)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Both syngap1b and shank3a play novel roles in morphogenesis resulting in common brain and behavioral phenotypes.
Title: Two knockdown models of the autism genes SYNGAP1 and SHANK3 in zebrafish produce similar behavioral phenotypes associated with embryonic disruptions of brain morphogenesis.

The following syngap1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the syngap1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa149171	XM_021466578.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa149182	XM_021466590.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa48971	XM_009292652.2 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa55356	XM_017351305.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa48962	NM_001326467.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa149170	XM_021466577.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa149172	XM_021466579.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa149173	XM_021466580.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa149174	XM_021466581.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa149175	XM_021466582.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa149176	XM_021466583.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa149177	XM_021466584.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa149177	XM_021466585.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa149178	XM_021466586.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa149179	XM_021466587.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa149180	XM_021466588.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa149181	XM_021466589.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa48963	XM_009292642.2 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa48964	XM_009292643.2 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa48965	XM_009292644.2 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa48966	XM_009292645.2 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa48967	XM_009292646.2 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa48968	XM_009292648.2 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa48969	XM_009292649.2 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa48970	XM_009292650.2 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa55357	XM_017351306.1 Latest version!	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

syngap1b ( XM_021466578.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322253.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa149171
Clone ID Related Accession (Same CDS sequence)	XM_021466578.1
Accession Version	XM_021466578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGCAG 
CAGATGGCAG GCATGGCATC GCAGACTGGC ACATCTCATT CATCTCTCGC CACTCCTCCT 
TCCACTGTTC AACCTGTTCG TCAAAGCTCC ATGGCTCCTC CCACGCAGCG CATGAAATCA 
CAACCCTCAC ATCAGCTTTC TGTGAGCTCT GCCGCTGGTG CCCCTGCGTC CAAAACCAGG 
CCGCAGAGCG GAAACCTGCT CCAGTCCCCG GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC 
CCTCGGCAGC AGCTGTCTGT CAAAGACAGC ACCCCACCCG GCCTGCCGCA TCAACAGAGC 
TCCACCAGGG AGAGTCAGGG ATCCCAGGGC TCTCAGGGTG GAACGCCGCA GTCCACGCAG 
CAGTCCAAAT CACATCAGGA ACGGCAGCAG GTCCAGCAAC AGCATCTGCT CAAGCCTACC 
ATGAGCAAAC AGGGCTCTTC ACAATCTCCA TCCACCCTGA ACCCGCAGAC CCCTGCCTCT 
GAGCGGACGG TCGCCTGGGT CTCCAACATG CCCCATCTTT CTGCTGACAT AGAGAGTTCC 
CGCATCGACA GAGAAGAGTT CAAGCTGAAG GAGTACTCCA AGAGCATGGA CGAGTCCCGT 
CTGGACCGGG TGCGCGAGTA TGAGGAGGAG ATCCACTCAC TGAAGGAGCG TCTGATGATG 
TCCCACCGCA AGTTGGAAGA GTACGAGAGG AGGCTGTTAA CTCAGGAGCA GCAGACCAAT 
AAAATCCTGC TGCAGTATCA GAGCCGACTG GACGACAGCG AGAGGAGACT CCGACAGCAG 
CAGATGGAGA AAGACAACCA AATTAAAGGA ATTATTGACA GACTCATGGC GGTGGAGGAC 
GAGTTGAGAG TAGGAGTGGT GCCTGAACAC AAACCCAGAG TATTTGCGGA TCAGGAAAAG 
CAGCTTTCCG CCTTGGGTTC AGCAGACCCC GGTGTGAGTT TTGGAGGAGA AATGACCAGC 
GCTTCTGGCT TTGTTGGAGT CTCCCCTTCG GACAAATCCT CCACCCGATC CCCCCAGAAT 
GGAATCGCGT CCTTCCCGTT AGTTCCCCCT CCTCGTTCAA TTCCTCACGC ACAACTCTGG 
CCGAACGGCA ACCAAAAATC TGCAATGGAT TGGAGGGCAA GTTTGGAGGG GGAGCAGGTC 
AATAACAACG CTAATCAAAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322253.1
CDS	127..4470
Translation	MLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQEKQLSALGSADPGVSFGGEMTSASGFVGVSPSDKSSTRSPQNGIASFPLVPPPRSIPHAQLWPNGNQKSAMDWRASLEGEQVNNNANQI

Target ORF information:

RefSeq Version	XM_021466578.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466578.1

ORF Insert Sequence:

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGCAG 
CAGATGGCAG GCATGGCATC GCAGACTGGC ACATCTCATT CATCTCTCGC CACTCCTCCT 
TCCACTGTTC AACCTGTTCG TCAAAGCTCC ATGGCTCCTC CCACGCAGCG CATGAAATCA 
CAACCCTCAC ATCAGCTTTC TGTGAGCTCT GCCGCTGGTG CCCCTGCGTC CAAAACCAGG 
CCGCAGAGCG GAAACCTGCT CCAGTCCCCG GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC 
CCTCGGCAGC AGCTGTCTGT CAAAGACAGC ACCCCACCCG GCCTGCCGCA TCAACAGAGC 
TCCACCAGGG AGAGTCAGGG ATCCCAGGGC TCTCAGGGTG GAACGCCGCA GTCCACGCAG 
CAGTCCAAAT CACATCAGGA ACGGCAGCAG GTCCAGCAAC AGCATCTGCT CAAGCCTACC 
ATGAGCAAAC AGGGCTCTTC ACAATCTCCA TCCACCCTGA ACCCGCAGAC CCCTGCCTCT 
GAGCGGACGG TCGCCTGGGT CTCCAACATG CCCCATCTTT CTGCTGACAT AGAGAGTTCC 
CGCATCGACA GAGAAGAGTT CAAGCTGAAG GAGTACTCCA AGAGCATGGA CGAGTCCCGT 
CTGGACCGGG TGCGCGAGTA TGAGGAGGAG ATCCACTCAC TGAAGGAGCG TCTGATGATG 
TCCCACCGCA AGTTGGAAGA GTACGAGAGG AGGCTGTTAA CTCAGGAGCA GCAGACCAAT 
AAAATCCTGC TGCAGTATCA GAGCCGACTG GACGACAGCG AGAGGAGACT CCGACAGCAG 
CAGATGGAGA AAGACAACCA AATTAAAGGA ATTATTGACA GACTCATGGC GGTGGAGGAC 
GAGTTGAGAG TAGGAGTGGT GCCTGAACAC AAACCCAGAG TATTTGCGGA TCAGGAAAAG 
CAGCTTTCCG CCTTGGGTTC AGCAGACCCC GGTGTGAGTT TTGGAGGAGA AATGACCAGC 
GCTTCTGGCT TTGTTGGAGT CTCCCCTTCG GACAAATCCT CCACCCGATC CCCCCAGAAT 
GGAATCGCGT CCTTCCCGTT AGTTCCCCCT CCTCGTTCAA TTCCTCACGC ACAACTCTGG 
CCGAACGGCA ACCAAAAATC TGCAATGGAT TGGAGGGCAA GTTTGGAGGG GGAGCAGGTC 
AATAACAACG CTAATCAAAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_021466590.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322265.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ODa149182
Clone ID Related Accession (Same CDS sequence)	XM_021466590.1
Accession Version	XM_021466590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGAGCAGCAG CAGATGGCAG GCATGGCATC GCAGACTGGC 
ACATCTCATT CATCTCTCGC CACTCCTCCT TCCACTGTTC AACCTGTTCG TCAAAGCTCC 
ATGGCTCCTC CCACGCAGCG CATGAAATCA CAACCCTCAC ATCAGCTTTC TGTGAGCTCT 
GCCGCTGGTG CCCCTGCGTC CAAAACCAGG CCGCAGAGCG GAAACCTGCT CCAGTCCCCG 
GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC CCTCGGCAGC AGCTGTCTGT CAAAGACAGC 
ACCCCACCCG GCCTGCCGCA TCAACAGAGC TCCACCAGGG AGAGTCAGGG ATCCCAGGGC 
TCTCAGGGTG GAACGCCGCA GTCCACGCAG CAGTCCAAAT CACATCAGGA ACGGCAGCAG 
GTCCAGCAAC AGCATCTGCT CAAGCCTACC ATGAGCAAAC AGGGCTCTTC ACAATCTCCA 
TCCACCCTGA ACCCGCAGAC CCCTGCCTCT GAGCGGACGG TCGCCTGGGT CTCCAACATG 
CCCCATCTTT CTGCTGACAT AGAGAGTTCC CGCATCGACA GAGAAGAGTT CAAGCTGAAG 
GAGTACTCCA AGAGCATGGA CGAGTCCCGT CTGGACCGGG TGCGCGAGTA TGAGGAGGAG 
ATCCACTCAC TGAAGGAGCG TCTGATGATG TCCCACCGCA AGTTGGAAGA GTACGAGAGG 
AGGCTGTTAA CTCAGGAGCA GCAGACCAAT AAAATCCTGC TGCAGTATCA GAGCCGACTG 
GACGACAGCG AGAGGAGACT CCGACAGCAG CAGATGGAGA AAGACAACCA AATTAAAGGA 
ATTATTGACA GACTCATGGC GGTGGAGGAC GAGTTGAGAG TAGGAGTGGT GCCTGAACAC 
AAACCCAGAG TATTTGCGGA TCAGGAAAAG CAGCTTTCCG CCTTGGGTTC AGCAGACCCC 
GGTGTGAGTT TTGGAGGAGA AATGACCAGC GCTTCTGGCT TTGTTGGAGT CTCCCCTTCG 
GACAAATCCT CCACCCGATC CCCCCAGAAT GGAATCGCGT CCTTCCCGTT AGTTCCCCCT 
CCTCGTTCAA TTCCTCACGC ACAACTCTGG CCGAACGGCA ACCAAAAATC TGCAATGGAT 
TGGAGGGCAA GTTTGGAGGG GGAGCAGGTC AATAACAACG CTAATCAAAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322265.1
CDS	127..3900
Translation	MLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQEKQLSALGSADPGVSFGGEMTSASGFVGVSPSDKSSTRSPQNGIASFPLVPPPRSIPHAQLWPNGNQKSAMDWRASLEGEQVNNNANQI

Target ORF information:

RefSeq Version	XM_021466590.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466590.1

ORF Insert Sequence:

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGAGCAGCAG CAGATGGCAG GCATGGCATC GCAGACTGGC 
ACATCTCATT CATCTCTCGC CACTCCTCCT TCCACTGTTC AACCTGTTCG TCAAAGCTCC 
ATGGCTCCTC CCACGCAGCG CATGAAATCA CAACCCTCAC ATCAGCTTTC TGTGAGCTCT 
GCCGCTGGTG CCCCTGCGTC CAAAACCAGG CCGCAGAGCG GAAACCTGCT CCAGTCCCCG 
GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC CCTCGGCAGC AGCTGTCTGT CAAAGACAGC 
ACCCCACCCG GCCTGCCGCA TCAACAGAGC TCCACCAGGG AGAGTCAGGG ATCCCAGGGC 
TCTCAGGGTG GAACGCCGCA GTCCACGCAG CAGTCCAAAT CACATCAGGA ACGGCAGCAG 
GTCCAGCAAC AGCATCTGCT CAAGCCTACC ATGAGCAAAC AGGGCTCTTC ACAATCTCCA 
TCCACCCTGA ACCCGCAGAC CCCTGCCTCT GAGCGGACGG TCGCCTGGGT CTCCAACATG 
CCCCATCTTT CTGCTGACAT AGAGAGTTCC CGCATCGACA GAGAAGAGTT CAAGCTGAAG 
GAGTACTCCA AGAGCATGGA CGAGTCCCGT CTGGACCGGG TGCGCGAGTA TGAGGAGGAG 
ATCCACTCAC TGAAGGAGCG TCTGATGATG TCCCACCGCA AGTTGGAAGA GTACGAGAGG 
AGGCTGTTAA CTCAGGAGCA GCAGACCAAT AAAATCCTGC TGCAGTATCA GAGCCGACTG 
GACGACAGCG AGAGGAGACT CCGACAGCAG CAGATGGAGA AAGACAACCA AATTAAAGGA 
ATTATTGACA GACTCATGGC GGTGGAGGAC GAGTTGAGAG TAGGAGTGGT GCCTGAACAC 
AAACCCAGAG TATTTGCGGA TCAGGAAAAG CAGCTTTCCG CCTTGGGTTC AGCAGACCCC 
GGTGTGAGTT TTGGAGGAGA AATGACCAGC GCTTCTGGCT TTGTTGGAGT CTCCCCTTCG 
GACAAATCCT CCACCCGATC CCCCCAGAAT GGAATCGCGT CCTTCCCGTT AGTTCCCCCT 
CCTCGTTCAA TTCCTCACGC ACAACTCTGG CCGAACGGCA ACCAAAAATC TGCAATGGAT 
TGGAGGGCAA GTTTGGAGGG GGAGCAGGTC AATAACAACG CTAATCAAAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_009292652.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290927.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ODa48971
Clone ID Related Accession (Same CDS sequence)	XM_009292652.2
Accession Version	XM_009292652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	si:dkey-246f6.1 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGAGCA GCAGCAGATG GCAGGCATGG CATCGCAGAC TGGCACATCT 
CATTCATCTC TCGCCACTCC TCCTTCCACT GTTCAACCTG TTCGTCAAAG CTCCATGGCT 
CCTCCCACGC AGCGCATGAA ATCACAACCC TCACATCAGC TTTCTGTGAG CTCTGCCGCT 
GGTGCCCCTG CGTCCAAAAC CAGGCCGCAG AGCGGAAACC TGCTCCAGTC CCCGGAGTCT 
GGCTTCGGGG GCCGGCAGCA GGGCCCTCGG CAGCAGCTGT CTGTCAAAGA CAGCACCCCA 
CCCGGCCTGC CGCATCAACA GAGCTCCACC AGGGAGAGTC AGGGATCCCA GGGCTCTCAG 
GGTGGAACGC CGCAGTCCAC GCAGCAGTCC AAATCACATC AGGAACGGCA GCAGGTCCAG 
CAACAGCATC TGCTCAAGCC TACCATGAGC AAACAGGGCT CTTCACAATC TCCATCCACC 
CTGAACCCGC AGACCCCTGC CTCTGAGCGG ACGGTCGCCT GGGTCTCCAA CATGCCCCAT 
CTTTCTGCTG ACATAGAGAG TTCCCGCATC GACAGAGAAG AGTTCAAGCT GAAGGAGTAC 
TCCAAGAGCA TGGACGAGTC CCGTCTGGAC CGGGTGCGCG AGTATGAGGA GGAGATCCAC 
TCACTGAAGG AGCGTCTGAT GATGTCCCAC CGCAAGTTGG AAGAGTACGA GAGGAGGCTG 
TTAACTCAGG AGCAGCAGAC CAATAAAATC CTGCTGCAGT ATCAGAGCCG ACTGGACGAC 
AGCGAGAGGA GACTCCGACA GCAGCAGATG GAGAAAGACA ACCAAATTAA AGGAATTATT 
GACAGACTCA TGGCGGTGGA GGACGAGTTG AGAGTAGGAG TGGTGCCTGA ACACAAACCC 
AGAGTATTTG CGGATCAGGA AAAGCAGCTT TCCGCCTTGG GTTCAGCAGA CCCCGGTGTG 
AGTTTTGGAG GAGAAATGAC CAGCGCTTCT GGCTTTGTTG GAGTCTCCCC TTCGGACAAA 
TCCTCCACCC GATCCCCCCA GAATGGAATC GCGTCCTTCC CGTTAGTTCC CCCTCCTCGT 
TCAATTCCTC ACGCACAACT CTGGCCGAAC GGCAACCAAA AATCTGCAAT GGATTGGAGG 
GCAAGTTTGG AGGGGGAGCA GGTCAATAAC AACGCTAATC AAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290927.1
CDS	316..4263
Translation	MSYVPFQDARYAVPPSYRPQPSFAAAPSYEQADWNPRLGVISGNQLYMLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKSSIRSWFIVSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQEKQLSALGSADPGVSFGGEMTSASGFVGVSPSDKSSTRSPQNGIASFPLVPPPRSIPHAQLWPNGNQKSAMDWRASLEGEQVNNNANQI

Target ORF information:

RefSeq Version	XM_009292652.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292652.2

ORF Insert Sequence:

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGAGCA GCAGCAGATG GCAGGCATGG CATCGCAGAC TGGCACATCT 
CATTCATCTC TCGCCACTCC TCCTTCCACT GTTCAACCTG TTCGTCAAAG CTCCATGGCT 
CCTCCCACGC AGCGCATGAA ATCACAACCC TCACATCAGC TTTCTGTGAG CTCTGCCGCT 
GGTGCCCCTG CGTCCAAAAC CAGGCCGCAG AGCGGAAACC TGCTCCAGTC CCCGGAGTCT 
GGCTTCGGGG GCCGGCAGCA GGGCCCTCGG CAGCAGCTGT CTGTCAAAGA CAGCACCCCA 
CCCGGCCTGC CGCATCAACA GAGCTCCACC AGGGAGAGTC AGGGATCCCA GGGCTCTCAG 
GGTGGAACGC CGCAGTCCAC GCAGCAGTCC AAATCACATC AGGAACGGCA GCAGGTCCAG 
CAACAGCATC TGCTCAAGCC TACCATGAGC AAACAGGGCT CTTCACAATC TCCATCCACC 
CTGAACCCGC AGACCCCTGC CTCTGAGCGG ACGGTCGCCT GGGTCTCCAA CATGCCCCAT 
CTTTCTGCTG ACATAGAGAG TTCCCGCATC GACAGAGAAG AGTTCAAGCT GAAGGAGTAC 
TCCAAGAGCA TGGACGAGTC CCGTCTGGAC CGGGTGCGCG AGTATGAGGA GGAGATCCAC 
TCACTGAAGG AGCGTCTGAT GATGTCCCAC CGCAAGTTGG AAGAGTACGA GAGGAGGCTG 
TTAACTCAGG AGCAGCAGAC CAATAAAATC CTGCTGCAGT ATCAGAGCCG ACTGGACGAC 
AGCGAGAGGA GACTCCGACA GCAGCAGATG GAGAAAGACA ACCAAATTAA AGGAATTATT 
GACAGACTCA TGGCGGTGGA GGACGAGTTG AGAGTAGGAG TGGTGCCTGA ACACAAACCC 
AGAGTATTTG CGGATCAGGA AAAGCAGCTT TCCGCCTTGG GTTCAGCAGA CCCCGGTGTG 
AGTTTTGGAG GAGAAATGAC CAGCGCTTCT GGCTTTGTTG GAGTCTCCCC TTCGGACAAA 
TCCTCCACCC GATCCCCCCA GAATGGAATC GCGTCCTTCC CGTTAGTTCC CCCTCCTCGT 
TCAATTCCTC ACGCACAACT CTGGCCGAAC GGCAACCAAA AATCTGCAAT GGATTGGAGG 
GCAAGTTTGG AGGGGGAGCA GGTCAATAAC AACGCTAATC AAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_017351305.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206794.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X1, mRNA.

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CloneID	ODa55356
Clone ID Related Accession (Same CDS sequence)	XM_017351305.1
Accession Version	XM_017351305.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	si:dkey-246f6.1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGATTC CAGAATGAAC AGCGTCTCTA ACCTGCAGTC TATGGGGGAC 
ATGCTCAACT CTTCCCAGGC CTCGATTGCT GGCCTCGGCA GCTACGGTGG GCTCGCAGGC 
TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA GCCGGAGGAC GTATGTCGGC CGGATCTGGC 
GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG AGCCAGCTGA GCCAGATGGG AACCACTACC 
GACTCCCTGT CCCAGCAGCA GCAGCAGCAG GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC 
CAGAACCCTC TGTTCCACCT CGCTGCTGAC GGACCGCAGC TTCACCACCA GCACAGTCGA 
GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC CCAGAGCCGG ACGGTTCTCA CCCTACTTAT 
ATCCCACAGT TTGCACATGG TGGTTTCTCC CGAAGTGAGG ACCTCTCCAC CCTCAGACCC 
GGCCCTCATC TGGGCCAGCC AAGTATTATT CATTCTCACA GCTACAGCGA CGACTACAGC 
CGTCATAACC AGAGCGACTA CGGGCGACGA CAGATCTCCA TGCACATGCA GCAGCAGATG 
GCAGGCATGG CATCGCAGAC TGGCACATCT CATTCATCTC TCGCCACTCC TCCTTCCACT 
GTTCAACCTG TTCGTCAAAG CTCCATGGCT CCTCCCACGC AGCGCATGAA ATCACAACCC 
TCACATCAGC TTTCTGTGAG CTCTGCCGCT GGTGCCCCTG CGTCCAAAAC CAGGCCGCAG 
AGCGGAAACC TGCTCCAGTC CCCGGAGTCT GGCTTCGGGG GCCGGCAGCA GGGCCCTCGG 
CAGCAGCTGT CTGTCAAAGA CAGCACCCCA CCCGGCCTGC CGCATCAACA GAGCTCCACC 
AGGGAGAGTC AGGGATCCCA GGGCTCTCAG GGTGGAACGC CGCAGTCCAC GCAGCAGTCC 
AAATCACATC AGGAACGGCA GCAGGTCCAG CAACAGCATC TGCTCAAGCC TACCATGAGC 
AAACAGGGCT CTTCACAATC TCCATCCACC CTGAACCCGC AGACCCCTGC CTCTGAGCGG 
ACGGTCGCCT GGGTCTCCAA CATGCCCCAT CTTTCTGCTG ACATAGAGAG TTCCCGCATC 
GACAGAGAAG AGTTCAAGCT GAAGGAGTAC TCCAAGAGCA TGGACGAGTC CCGTCTGGAC 
CGGGTGCGCG AGTATGAGGA GGAGATCCAC TCACTGAAGG AGCGTCTGAT GATGTCCCAC 
CGCAAGTTGG AAGAGTACGA GAGGAGGCTG TTAACTCAGG AGCAGCAGAC CAATAAAATC 
CTGCTGCAGT ATCAGAGCCG ACTGGACGAC AGCGAGAGGA GACTCCGACA GCAGCAGATG 
GAGAAAGACA ACCAAATTAA AGGAATTATT GACAGACTCA TGGCGGTGGA GGACGAGTTG 
AGAGTAGGAG TGGTGCCTGA ACACAAACCC AGAGTATTTG CGGATCAGGA AAAGCAGCTT 
TCCGCCTTGG GTTCAGCAGA CCCCGGTGTG AGTTTTGGAG GAGAAATGAC CAGCGCTTCT 
GGCTTTGTTG GAGTCTCCCC TTCGGACAAA TCCTCCACCC GATCCCCCCA GAATGGAATC 
GCGTCCTTCC CGTTAGTTCC CCCTCCTCGT TCAATTCCTC ACGCACAACT CTGGCCGAAC 
GGCAACCAAA AATCTGCAAT GGATTGGAGG GCAAGTTTGG AGGGGGAGCA GGTCAATAAC 
AACGCTAATC AAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206794.1
CDS	316..4833
Translation	MSYVPFQDARYAVPPSYRPQPSFAAAPSYEQADWNPRLGVISGNQLYMLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKSSIRSWFIVSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQEKQLSALGSADPGVSFGGEMTSASGFVGVSPSDKSSTRSPQNGIASFPLVPPPRSIPHAQLWPNGNQKSAMDWRASLEGEQVNNNANQI

Target ORF information:

RefSeq Version	XM_017351305.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351305.1

ORF Insert Sequence:

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGATTC CAGAATGAAC AGCGTCTCTA ACCTGCAGTC TATGGGGGAC 
ATGCTCAACT CTTCCCAGGC CTCGATTGCT GGCCTCGGCA GCTACGGTGG GCTCGCAGGC 
TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA GCCGGAGGAC GTATGTCGGC CGGATCTGGC 
GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG AGCCAGCTGA GCCAGATGGG AACCACTACC 
GACTCCCTGT CCCAGCAGCA GCAGCAGCAG GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC 
CAGAACCCTC TGTTCCACCT CGCTGCTGAC GGACCGCAGC TTCACCACCA GCACAGTCGA 
GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC CCAGAGCCGG ACGGTTCTCA CCCTACTTAT 
ATCCCACAGT TTGCACATGG TGGTTTCTCC CGAAGTGAGG ACCTCTCCAC CCTCAGACCC 
GGCCCTCATC TGGGCCAGCC AAGTATTATT CATTCTCACA GCTACAGCGA CGACTACAGC 
CGTCATAACC AGAGCGACTA CGGGCGACGA CAGATCTCCA TGCACATGCA GCAGCAGATG 
GCAGGCATGG CATCGCAGAC TGGCACATCT CATTCATCTC TCGCCACTCC TCCTTCCACT 
GTTCAACCTG TTCGTCAAAG CTCCATGGCT CCTCCCACGC AGCGCATGAA ATCACAACCC 
TCACATCAGC TTTCTGTGAG CTCTGCCGCT GGTGCCCCTG CGTCCAAAAC CAGGCCGCAG 
AGCGGAAACC TGCTCCAGTC CCCGGAGTCT GGCTTCGGGG GCCGGCAGCA GGGCCCTCGG 
CAGCAGCTGT CTGTCAAAGA CAGCACCCCA CCCGGCCTGC CGCATCAACA GAGCTCCACC 
AGGGAGAGTC AGGGATCCCA GGGCTCTCAG GGTGGAACGC CGCAGTCCAC GCAGCAGTCC 
AAATCACATC AGGAACGGCA GCAGGTCCAG CAACAGCATC TGCTCAAGCC TACCATGAGC 
AAACAGGGCT CTTCACAATC TCCATCCACC CTGAACCCGC AGACCCCTGC CTCTGAGCGG 
ACGGTCGCCT GGGTCTCCAA CATGCCCCAT CTTTCTGCTG ACATAGAGAG TTCCCGCATC 
GACAGAGAAG AGTTCAAGCT GAAGGAGTAC TCCAAGAGCA TGGACGAGTC CCGTCTGGAC 
CGGGTGCGCG AGTATGAGGA GGAGATCCAC TCACTGAAGG AGCGTCTGAT GATGTCCCAC 
CGCAAGTTGG AAGAGTACGA GAGGAGGCTG TTAACTCAGG AGCAGCAGAC CAATAAAATC 
CTGCTGCAGT ATCAGAGCCG ACTGGACGAC AGCGAGAGGA GACTCCGACA GCAGCAGATG 
GAGAAAGACA ACCAAATTAA AGGAATTATT GACAGACTCA TGGCGGTGGA GGACGAGTTG 
AGAGTAGGAG TGGTGCCTGA ACACAAACCC AGAGTATTTG CGGATCAGGA AAAGCAGCTT 
TCCGCCTTGG GTTCAGCAGA CCCCGGTGTG AGTTTTGGAG GAGAAATGAC CAGCGCTTCT 
GGCTTTGTTG GAGTCTCCCC TTCGGACAAA TCCTCCACCC GATCCCCCCA GAATGGAATC 
GCGTCCTTCC CGTTAGTTCC CCCTCCTCGT TCAATTCCTC ACGCACAACT CTGGCCGAAC 
GGCAACCAAA AATCTGCAAT GGATTGGAGG GCAAGTTTGG AGGGGGAGCA GGTCAATAAC 
AACGCTAATC AAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( NM_001326467.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001313396.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), mRNA.

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CloneID	ODa48962
Clone ID Related Accession (Same CDS sequence)	NM_001326467.1
Accession Version	NM_001326467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP016083.11, CU062666.7 and CU459129.3. On May 19, 2016 this sequence version replaced XM_009292641.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript was available for the full length of the gene. The extent of this transcript is supported by RNA-seq and orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGATTC CAGAATGAAC AGCGTCTCTA ACCTGCAGTC TATGGGGGAC 
ATGCTCAACT CTTCCCAGGC CTCGATTGCT GGCCTCGGCA GCTACGGTGG GCTCGCAGGC 
TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA GCCGGAGGAC GTATGTCGGC CGGATCTGGC 
GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG AGCCAGCTGA GCCAGATGGG AACCACTACC 
GACTCCCTGT CCCAGCAGCA GCAGCAGCAG GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC 
CAGAACCCTC TGTTCCACCT CGCTGCTGAC GGACCGCAGC TTCACCACCA GCACAGTCGA 
GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC CCAGAGCCGG ACGGTTCTCA CCCTACTTAT 
ATCCCACAGT TTGCACATGG TGGTTTCTCC CGAAGTGAGG ACCTCTCCAC CCTCAGACCC 
GGCCCTCATC TGGGCCAGCC AAGTATTATT CATTCTCACA GCTACAGCGA CGACTACAGC 
CGTCATAACC AGAGCGACTA CGGGCGACGA CAGATCTCCA TGCACATGCA GGAGCAGCAG 
CAGATGGCAG GCATGGCATC GCAGACTGGC ACATCTCATT CATCTCTCGC CACTCCTCCT 
TCCACTGTTC AACCTGTTCG TCAAAGCTCC ATGGCTCCTC CCACGCAGCG CATGAAATCA 
CAACCCTCAC ATCAGCTTTC TGTGAGCTCT GCCGCTGGTG CCCCTGCGTC CAAAACCAGG 
CCGCAGAGCG GAAACCTGCT CCAGTCCCCG GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC 
CCTCGGCAGC AGCTGTCTGT CAAAGACAGC ACCCCACCCG GCCTGCCGCA TCAACAGAGC 
TCCACCAGGG AGAGTCAGGG ATCCCAGGGC TCTCAGGGTG GAACGCCGCA GTCCACGCAG 
CAGTCCAAAT CACATCAGGA ACGGCAGCAG GTCCAGCAAC AGCATCTGCT CAAGCCTACC 
ATGAGCAAAC AGGGCTCTTC ACAATCTCCA TCCACCCTGA ACCCGCAGAC CCCTGCCTCT 
GAGCGGACGG TCGCCTGGGT CTCCAACATG CCCCATCTTT CTGCTGACAT AGAGAGTTCC 
CGCATCGACA GAGAAGAGTT CAAGCTGAAG GAGTACTCCA AGAGCATGGA CGAGTCCCGT 
CTGGACCGGG TGCGCGAGTA TGAGGAGGAG ATCCACTCAC TGAAGGAGCG TCTGATGATG 
TCCCACCGCA AGTTGGAAGA GTACGAGAGG AGGCTGTTAA CTCAGGAGCA GCAGACCAAT 
AAAATCCTGC TGCAGTATCA GAGCCGACTG GACGACAGCG AGAGGAGACT CCGACAGCAG 
CAGATGGAGA AAGACAACCA AATTAAAGGA ATTATTGACA GACTCATGGC GGTGGAGGAC 
GAGTTGAGAG TAGGAGTGGT GCCTGAACAC AAACCCAGAG TATTTGCGGA TCAGGAAAAG 
CAGCTTTCCG CCTTGGGTTC AGCAGACCCC GGTGTGAGTT TTGGAGGAGA AATGACCAGC 
GCTTCTGGCT TTGTTGGAGT CTCCCCTTCG GACAAATCCT CCACCCGATC CCCCCAGAAT 
GGAATCGCGT CCTTCCCGTT AGTTCCCCCT CCTCGTTCAA TTCCTCACGC ACAACTCTGG 
CCGAACGGCA ACCAAAAATC TGCAATGGAT TGGAGGGCAA GTTTGGAGGG GGAGCAGGTC 
AATAACAACG CTAATCAAAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001313396.1
CDS	319..4842
Translation	MSYVPFQDARYAVPPSYRPQPSFAAAPSYEQADWNPRLGVISGNQLYMLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKSSIRSWFIVSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQEKQLSALGSADPGVSFGGEMTSASGFVGVSPSDKSSTRSPQNGIASFPLVPPPRSIPHAQLWPNGNQKSAMDWRASLEGEQVNNNANQI

Target ORF information:

RefSeq Version	NM_001326467.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001326467.1

ORF Insert Sequence:

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGATTC CAGAATGAAC AGCGTCTCTA ACCTGCAGTC TATGGGGGAC 
ATGCTCAACT CTTCCCAGGC CTCGATTGCT GGCCTCGGCA GCTACGGTGG GCTCGCAGGC 
TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA GCCGGAGGAC GTATGTCGGC CGGATCTGGC 
GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG AGCCAGCTGA GCCAGATGGG AACCACTACC 
GACTCCCTGT CCCAGCAGCA GCAGCAGCAG GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC 
CAGAACCCTC TGTTCCACCT CGCTGCTGAC GGACCGCAGC TTCACCACCA GCACAGTCGA 
GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC CCAGAGCCGG ACGGTTCTCA CCCTACTTAT 
ATCCCACAGT TTGCACATGG TGGTTTCTCC CGAAGTGAGG ACCTCTCCAC CCTCAGACCC 
GGCCCTCATC TGGGCCAGCC AAGTATTATT CATTCTCACA GCTACAGCGA CGACTACAGC 
CGTCATAACC AGAGCGACTA CGGGCGACGA CAGATCTCCA TGCACATGCA GGAGCAGCAG 
CAGATGGCAG GCATGGCATC GCAGACTGGC ACATCTCATT CATCTCTCGC CACTCCTCCT 
TCCACTGTTC AACCTGTTCG TCAAAGCTCC ATGGCTCCTC CCACGCAGCG CATGAAATCA 
CAACCCTCAC ATCAGCTTTC TGTGAGCTCT GCCGCTGGTG CCCCTGCGTC CAAAACCAGG 
CCGCAGAGCG GAAACCTGCT CCAGTCCCCG GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC 
CCTCGGCAGC AGCTGTCTGT CAAAGACAGC ACCCCACCCG GCCTGCCGCA TCAACAGAGC 
TCCACCAGGG AGAGTCAGGG ATCCCAGGGC TCTCAGGGTG GAACGCCGCA GTCCACGCAG 
CAGTCCAAAT CACATCAGGA ACGGCAGCAG GTCCAGCAAC AGCATCTGCT CAAGCCTACC 
ATGAGCAAAC AGGGCTCTTC ACAATCTCCA TCCACCCTGA ACCCGCAGAC CCCTGCCTCT 
GAGCGGACGG TCGCCTGGGT CTCCAACATG CCCCATCTTT CTGCTGACAT AGAGAGTTCC 
CGCATCGACA GAGAAGAGTT CAAGCTGAAG GAGTACTCCA AGAGCATGGA CGAGTCCCGT 
CTGGACCGGG TGCGCGAGTA TGAGGAGGAG ATCCACTCAC TGAAGGAGCG TCTGATGATG 
TCCCACCGCA AGTTGGAAGA GTACGAGAGG AGGCTGTTAA CTCAGGAGCA GCAGACCAAT 
AAAATCCTGC TGCAGTATCA GAGCCGACTG GACGACAGCG AGAGGAGACT CCGACAGCAG 
CAGATGGAGA AAGACAACCA AATTAAAGGA ATTATTGACA GACTCATGGC GGTGGAGGAC 
GAGTTGAGAG TAGGAGTGGT GCCTGAACAC AAACCCAGAG TATTTGCGGA TCAGGAAAAG 
CAGCTTTCCG CCTTGGGTTC AGCAGACCCC GGTGTGAGTT TTGGAGGAGA AATGACCAGC 
GCTTCTGGCT TTGTTGGAGT CTCCCCTTCG GACAAATCCT CCACCCGATC CCCCCAGAAT 
GGAATCGCGT CCTTCCCGTT AGTTCCCCCT CCTCGTTCAA TTCCTCACGC ACAACTCTGG 
CCGAACGGCA ACCAAAAATC TGCAATGGAT TGGAGGGCAA GTTTGGAGGG GGAGCAGGTC 
AATAACAACG CTAATCAAAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_021466577.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322252.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa149170
Clone ID Related Accession (Same CDS sequence)	XM_021466577.1
Accession Version	XM_021466577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGACT CATGGCGGTG 
GAGGACGAGT TGAGAGTAGG AGTGGTGCCT GAACACAAAC CCAGAGTATT TGCGGATCAG 
GAAAAGCAGC TTTCCGCCTT GGGTTCAGCA GACCCCGGTG TGAGTTTTGG AGGAGAAATG 
ACCAGCGCTT CTGGCTTTGT TGGAGTCTCC CCTTCGGACA AATCCTCCAC CCGATCCCCC 
CAGAATGGAA TCGCGTCCTT CCCGTTAGTT CCCCCTCCTC GTTCAATTCC TCACGCACAA 
CTCTGGCCGA ACGGCAACCA AAAATCTGCA ATGGATTGGA GGGCAAGTTT GGAGGGGGAG 
CAGGTCAATA ACAACGCTAA TCAAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322252.1
CDS	127..4476
Translation	MLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQEKQLSALGSADPGVSFGGEMTSASGFVGVSPSDKSSTRSPQNGIASFPLVPPPRSIPHAQLWPNGNQKSAMDWRASLEGEQVNNNANQI

Target ORF information:

RefSeq Version	XM_021466577.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466577.1

ORF Insert Sequence:

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGACT CATGGCGGTG 
GAGGACGAGT TGAGAGTAGG AGTGGTGCCT GAACACAAAC CCAGAGTATT TGCGGATCAG 
GAAAAGCAGC TTTCCGCCTT GGGTTCAGCA GACCCCGGTG TGAGTTTTGG AGGAGAAATG 
ACCAGCGCTT CTGGCTTTGT TGGAGTCTCC CCTTCGGACA AATCCTCCAC CCGATCCCCC 
CAGAATGGAA TCGCGTCCTT CCCGTTAGTT CCCCCTCCTC GTTCAATTCC TCACGCACAA 
CTCTGGCCGA ACGGCAACCA AAAATCTGCA ATGGATTGGA GGGCAAGTTT GGAGGGGGAG 
CAGGTCAATA ACAACGCTAA TCAAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_021466579.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322254.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa149172
Clone ID Related Accession (Same CDS sequence)	XM_021466579.1
Accession Version	XM_021466579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGCCTCGATT GCTGGCCTCG GCAGCTACGG TGGGCTCGCA 
GGCTTGGGTG GAGGCCTCGG AGGTCAGCTG CGAGCCGGAG GACGTATGTC GGCCGGATCT 
GGCGGTTCCA GTATGTCCGG GGGTCTGAGG TTGAGCCAGC TGAGCCAGAT GGGAACCACT 
ACCGACTCCC TGTCCCAGCA GCAGCAGCAG CAGGCCGCCG CCCTCCGCTT TCCCCTGTCC 
TTCCAGAACC CTCTGTTCCA CCTCGCTGCT GACGGACCGC AGCTTCACCA CCAGCACAGT 
CGAGCTCAGC CTCCTCCTCC GCTCCTGCTG GCCCCAGAGC CGGACGGTTC TCACCCTACT 
TATATCCCAC AGTTTGCACA TGGTGGTTTC TCCCGAAGTG AGGACCTCTC CACCCTCAGA 
CCCGGCCCTC ATCTGGGCCA GCCAAGTATT ATTCATTCTC ACAGCTACAG CGACGACTAC 
AGCCGTCATA ACCAGAGCGA CTACGGGCGA CGACAGATCT CCATGCACAT GCAGGAGCAG 
CAGCAGATGG CAGGCATGGC ATCGCAGACT GGCACATCTC ATTCATCTCT CGCCACTCCT 
CCTTCCACTG TTCAACCTGT TCGTCAAAGC TCCATGGCTC CTCCCACGCA GCGCATGAAA 
TCACAACCCT CACATCAGCT TTCTGTGAGC TCTGCCGCTG GTGCCCCTGC GTCCAAAACC 
AGGCCGCAGA GCGGAAACCT GCTCCAGTCC CCGGAGTCTG GCTTCGGGGG CCGGCAGCAG 
GGCCCTCGGC AGCAGCTGTC TGTCAAAGAC AGCACCCCAC CCGGCCTGCC GCATCAACAG 
AGCTCCACCA GGGAGAGTCA GGGATCCCAG GGCTCTCAGG GTGGAACGCC GCAGTCCACG 
CAGCAGTCCA AATCACATCA GGAACGGCAG CAGGTCCAGC AACAGCATCT GCTCAAGCCT 
ACCATGAGCA AACAGGGCTC TTCACAATCT CCATCCACCC TGAACCCGCA GACCCCTGCC 
TCTGAGCGGA CGGTCGCCTG GGTCTCCAAC ATGCCCCATC TTTCTGCTGA CATAGAGAGT 
TCCCGCATCG ACAGAGAAGA GTTCAAGCTG AAGGAGTACT CCAAGAGCAT GGACGAGTCC 
CGTCTGGACC GGGTGCGCGA GTATGAGGAG GAGATCCACT CACTGAAGGA GCGTCTGATG 
ATGTCCCACC GCAAGTTGGA AGAGTACGAG AGGAGGCTGT TAACTCAGGA GCAGCAGACC 
AATAAAATCC TGCTGCAGTA TCAGAGCCGA CTGGACGACA GCGAGAGGAG ACTCCGACAG 
CAGCAGATGG AGAAAGACAA CCAAATTAAA GGAATTATTG ACAGACTCAT GGCGGTGGAG 
GACGAGTTGA GAGTAGGAGT GGTGCCTGAA CACAAACCCA GAGTATTTGC GGATCAGGAA 
AAGCAGCTTT CCGCCTTGGG TTCAGCAGAC CCCGGTGTGA GTTTTGGAGG AGAAATGACC 
AGCGCTTCTG GCTTTGTTGG AGTCTCCCCT TCGGACAAAT CCTCCACCCG ATCCCCCCAG 
AATGGAATCG CGTCCTTCCC GTTAGTTCCC CCTCCTCGTT CAATTCCTCA CGCACAACTC 
TGGCCGAACG GCAACCAAAA ATCTGCAATG GATTGGAGGG CAAGTTTGGA GGGGGAGCAG 
GTCAATAACA ACGCTAATCA AATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322254.1
CDS	127..4413
Translation	MLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQEKQLSALGSADPGVSFGGEMTSASGFVGVSPSDKSSTRSPQNGIASFPLVPPPRSIPHAQLWPNGNQKSAMDWRASLEGEQVNNNANQI

Target ORF information:

RefSeq Version	XM_021466579.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466579.1

ORF Insert Sequence:

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGCCTCGATT GCTGGCCTCG GCAGCTACGG TGGGCTCGCA 
GGCTTGGGTG GAGGCCTCGG AGGTCAGCTG CGAGCCGGAG GACGTATGTC GGCCGGATCT 
GGCGGTTCCA GTATGTCCGG GGGTCTGAGG TTGAGCCAGC TGAGCCAGAT GGGAACCACT 
ACCGACTCCC TGTCCCAGCA GCAGCAGCAG CAGGCCGCCG CCCTCCGCTT TCCCCTGTCC 
TTCCAGAACC CTCTGTTCCA CCTCGCTGCT GACGGACCGC AGCTTCACCA CCAGCACAGT 
CGAGCTCAGC CTCCTCCTCC GCTCCTGCTG GCCCCAGAGC CGGACGGTTC TCACCCTACT 
TATATCCCAC AGTTTGCACA TGGTGGTTTC TCCCGAAGTG AGGACCTCTC CACCCTCAGA 
CCCGGCCCTC ATCTGGGCCA GCCAAGTATT ATTCATTCTC ACAGCTACAG CGACGACTAC 
AGCCGTCATA ACCAGAGCGA CTACGGGCGA CGACAGATCT CCATGCACAT GCAGGAGCAG 
CAGCAGATGG CAGGCATGGC ATCGCAGACT GGCACATCTC ATTCATCTCT CGCCACTCCT 
CCTTCCACTG TTCAACCTGT TCGTCAAAGC TCCATGGCTC CTCCCACGCA GCGCATGAAA 
TCACAACCCT CACATCAGCT TTCTGTGAGC TCTGCCGCTG GTGCCCCTGC GTCCAAAACC 
AGGCCGCAGA GCGGAAACCT GCTCCAGTCC CCGGAGTCTG GCTTCGGGGG CCGGCAGCAG 
GGCCCTCGGC AGCAGCTGTC TGTCAAAGAC AGCACCCCAC CCGGCCTGCC GCATCAACAG 
AGCTCCACCA GGGAGAGTCA GGGATCCCAG GGCTCTCAGG GTGGAACGCC GCAGTCCACG 
CAGCAGTCCA AATCACATCA GGAACGGCAG CAGGTCCAGC AACAGCATCT GCTCAAGCCT 
ACCATGAGCA AACAGGGCTC TTCACAATCT CCATCCACCC TGAACCCGCA GACCCCTGCC 
TCTGAGCGGA CGGTCGCCTG GGTCTCCAAC ATGCCCCATC TTTCTGCTGA CATAGAGAGT 
TCCCGCATCG ACAGAGAAGA GTTCAAGCTG AAGGAGTACT CCAAGAGCAT GGACGAGTCC 
CGTCTGGACC GGGTGCGCGA GTATGAGGAG GAGATCCACT CACTGAAGGA GCGTCTGATG 
ATGTCCCACC GCAAGTTGGA AGAGTACGAG AGGAGGCTGT TAACTCAGGA GCAGCAGACC 
AATAAAATCC TGCTGCAGTA TCAGAGCCGA CTGGACGACA GCGAGAGGAG ACTCCGACAG 
CAGCAGATGG AGAAAGACAA CCAAATTAAA GGAATTATTG ACAGACTCAT GGCGGTGGAG 
GACGAGTTGA GAGTAGGAGT GGTGCCTGAA CACAAACCCA GAGTATTTGC GGATCAGGAA 
AAGCAGCTTT CCGCCTTGGG TTCAGCAGAC CCCGGTGTGA GTTTTGGAGG AGAAATGACC 
AGCGCTTCTG GCTTTGTTGG AGTCTCCCCT TCGGACAAAT CCTCCACCCG ATCCCCCCAG 
AATGGAATCG CGTCCTTCCC GTTAGTTCCC CCTCCTCGTT CAATTCCTCA CGCACAACTC 
TGGCCGAACG GCAACCAAAA ATCTGCAATG GATTGGAGGG CAAGTTTGGA GGGGGAGCAG 
GTCAATAACA ACGCTAATCA AATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_021466580.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322255.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X4, mRNA.

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CloneID	ODa149173
Clone ID Related Accession (Same CDS sequence)	XM_021466580.1
Accession Version	XM_021466580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGACT CATGGCGGTG 
GAGGACGAGT TGAGAGTAGG AGTGGTGCCT GAACACAAAC CCAGAGTATT TGCGGATCAG 
GGGAGGCGGC AGTCCATCCT AGTGCAGCCC CGCCTTGCGC CCGTCCCACT GCGAATGCAC 
AGCCAACAAC AAAGCTTCAT GGAGGACAAT CTGGAACCTA ATTGGAGGCC TCACCTAGGA 
CATTCTTCAG GTTGGCCTGG GTTTAATTCC AGGGCGCTGT CACGCGATTC GGTTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322255.1
CDS	127..4386
Translation	MLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQGRRQSILVQPRLAPVPLRMHSQQQSFMEDNLEPNWRPHLGHSSGWPGFNSRALSRDSVG

Target ORF information:

RefSeq Version	XM_021466580.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466580.1

ORF Insert Sequence:

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGACT CATGGCGGTG 
GAGGACGAGT TGAGAGTAGG AGTGGTGCCT GAACACAAAC CCAGAGTATT TGCGGATCAG 
GGGAGGCGGC AGTCCATCCT AGTGCAGCCC CGCCTTGCGC CCGTCCCACT GCGAATGCAC 
AGCCAACAAC AAAGCTTCAT GGAGGACAAT CTGGAACCTA ATTGGAGGCC TCACCTAGGA 
CATTCTTCAG GTTGGCCTGG GTTTAATTCC AGGGCGCTGT CACGCGATTC GGTTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_021466581.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322256.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X5, mRNA.

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CloneID	ODa149174
Clone ID Related Accession (Same CDS sequence)	XM_021466581.1
Accession Version	XM_021466581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGACT CATGGCGGTG 
GAGGACGAGT TGAGAGTAGG AGTGGTGCCT GAACACAAAC CCAGAGTATT TGCGGATCAG 
GGGAGGCGGC AGTCCATCCT AGTGCAGCCC CGCCTTGCGC CCGTCCCACT GCGAATGCAC 
AGGAAAAGCA GCTTTCCGCC TTGGGTTCAG CAGACCCCGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322256.1
CDS	127..4311
Translation	MLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQGRRQSILVQPRLAPVPLRMHRKSSFPPWVQQTPV

Target ORF information:

RefSeq Version	XM_021466581.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466581.1

ORF Insert Sequence:

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGACT CATGGCGGTG 
GAGGACGAGT TGAGAGTAGG AGTGGTGCCT GAACACAAAC CCAGAGTATT TGCGGATCAG 
GGGAGGCGGC AGTCCATCCT AGTGCAGCCC CGCCTTGCGC CCGTCCCACT GCGAATGCAC 
AGGAAAAGCA GCTTTCCGCC TTGGGTTCAG CAGACCCCGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_021466582.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322257.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa149175
Clone ID Related Accession (Same CDS sequence)	XM_021466582.1
Accession Version	XM_021466582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATCC CACCCACTGC CCCCCCACAA CCCTTCCGAC AATCCGTGAG TAACCCACCT 
TTCCGTCCCC GGTCCCTCTC TCCATCCCTG TCCCTATCGC TGCCGTTCGC TCCATCGCCC 
CTGTCGCCCT CGCTGTCCCC GTCCCATTTA CAGTCCCAAA AGCGAATCAG CACTCCTCCG 
CTGCCTCGTT CTCTCCCTCT CTCCCCGAGC GTCTCTCTGA CTCTCCCACC CTCTGTCCCG 
CTCTCCCACT GGGCTGCTGG TGAGTGGAAG GTTGTGGGTT CTAATCCAGG GACGGGATCC 
AGTGTGGCGG CACTGCCCTC TCTTGAGAGT TTCCTCAGTA GAAGACTGAA GGGGTCCATT 
AAACGGGCCA AGAGTCAGCC CAAGCTGGAC CGAACCAGCA GCTTCCGCCA CATGATCCTC 
CCTCGCTTCC GCAGCGCCGA CCAGGAGCGG ACTCGTCTGA TGCAGAGCTT CAAAGAATCA 
CACTCCCATG AGTCTTTGCT GTCTCCCAGC AGCGCAGCCG AGGCCTTGGA TTTGACACTT 
GATGAAGACG CCATCATCAA GCCTGTTCAC TCCAGTATAT TGGGACAGGA GTACTGTTTC 
GAGGTGATAA CTGCTTCAGG AACAAAGTGT TTTGCCTGTC GGTCGGCTGC GGAGCGAGAC 
AAATGGATCG AGAATCTACA GAGAGCTGTC AAACCCAACA AAGACAACAG TCGGCGTGTC 
GACAACGTCC TGAAGCTGTG GATCATTGAG GCCCGTGAGC TCCCACCCAA AAAGCGCTAT 
TATTGTGAAC TGTGCTTGGA TGACATGCTG TACGCTCGCA CCACCAGCAA ACCCCGTACC 
GACACTGTCT TCTGGGGTGA GCACTTTGAG TTCAACAACC TGCCTGCTGT CCGCAACCTC 
CGCCTTCATC TGTACAAAGA GACGGACAAG AAGAGACGCA AGGAGAAGAG CACGTACCTT 
GGCTTGGTCA GCATTCCCAT TTCCAGCATC ACTGGCCGTC AGTTTGTGGA GCAATGGTAC 
CCCGTCATTC AGCCCAGTGT TCTGGCCAAG GGTGGCGGCG TCGGAGGAGG GAAGATCATC 
AATGCCTCGC TGCGGCTCAA GTCACGCTTT CAGACCATGA GCATCCTGCC GATGGAACTG 
TATAAAGAGT TCGCAGAGTA CGTCACAAAC AACTACCGGA CGCTGTGTGC GGTCCTGGAG 
CCACTGCTGA GTGTCAAGAG CAAAGAAGAA GTGGCCTGTG CTCTGGTGCA CATCCTACAG 
AGCACAGGGA AGGCCAAGGA TTTTCTGTCA GACATGGCGA TGTGTGAGGT GGATCGCTTC 
ATCGACCGCG AGCACCTGAT CTTCAGAGAA AACACCCTGG CCACCAAAGC CATAGAAGAG 
TACCTCAAAC TCATCGGACA CAAGTACCTC AAAGATGTTT TAGGTGATTT TATCCGAGCG 
CTGTATGAAT CAGAGGAGAA CTGTGAAGTG GACCCGATGC GAACTCCCCC TTCAATACTG 
CCCGAACATC AAGCCAACCT CCGGATGTGC TGTGAGCTCG CCCTCTGCAA AATCATCAAC 
TCATATTGTG CGTTCCCGCG AGAGCTGAAG GAAGTGTTTG CATCATGGCG TGTCCGCTGT 
GCTGAGAGAG GAAGAGAAGA CATTGCAGAT CGACTGATCA GCGGCTCCCT CTTCCTGCGC 
TTCCTCTGTC CAGCCATCAT GTCTCCATCT CTGTTCAACC TCACGCAGGA ATATCCCGAT 
GAGCAGACCT CACGAACACT CACACTCATC GCCAAAGTGG TCCAGAACCT CGCAAACTTC 
TCCAAGTTTG GCAGTAAGGA GGAGTACATG TGCTTCATGA ATGAGTTTCT GGAGATGGAG 
TGGGGCTCCA TGCAGCAGTT TCTCTATGAG ATCTCCAATC TGGACAGTGT GAGCAATGCT 
GGGGCCTTCG AGGGCTACAT TGACCTAGGC CGCGAGCTCT CCATCCTGCA CAGTCTGCTC 
TGGGAGGTTA TGGCTCAGCT GAGCAAGGAT GCCATCATCA AACTTGGACC ATTGCCCCGC 
CTCCTCAATG ACATCAGCAT GGCCCTCCGC AACCCTCACC TGCAGAGGCA GCCCAGTCAT 
CAGACAGACA GACAGCCTGC AGAGAGACAG ACAGACCGAC TGCTGTCCCG ACCCAGCTTC 
AACAGGGGCG TCTCGTCTGA GTTCCAGAAT CTCATGATGA GAGACCTTAA CAGTTCAGTG 
GAGATCACTC GTCTGCCCTC TCCAACTTCT GCCATGTCCA GCGGTGGAGC TCCTCCATCC 
CGTGCAGGAA TGGCTGGGTT TGCGGACCGC GATCATCCTC ACCGAGCCTC GTCTAAAGAT 
GTTTTCTATG GTGCCCGTCC CCCACTGGCC CGCTCCAGTC CAGCGTACTG TACTAGCAGT 
TCAGATATTA CTGATCCAGA CCCAAAGGAT TCCAGAATGA ACAGCGTCTC TAACCTGCAG 
TCTATGGGGG ACATGCTCAA CTCTTCCCAG GCCTCGATTG CTGGCCTCGG CAGCTACGGT 
GGGCTCGCAG GCTTGGGTGG AGGCCTCGGA GGTCAGCTGC GAGCCGGAGG ACGTATGTCG 
GCCGGATCTG GCGGTTCCAG TATGTCCGGG GGTCTGAGGT TGAGCCAGCT GAGCCAGATG 
GGAACCACTA CCGACTCCCT GTCCCAGCAG CAGCAGCAGC AGGCCGCCGC CCTCCGCTTT 
CCCCTGTCCT TCCAGAACCC TCTGTTCCAC CTCGCTGCTG ACGGACCGCA GCTTCACCAC 
CAGCACAGTC GAGCTCAGCC TCCTCCTCCG CTCCTGCTGG CCCCAGAGCC GGACGGTTCT 
CACCCTACTT ATATCCCACA GTTTGCACAT GGTGGTTTCT CCCGAAGTGA GGACCTCTCC 
ACCCTCAGAC CCGGCCCTCA TCTGGGCCAG CCAAGTATTA TTCATTCTCA CAGCTACAGC 
GACGACTACA GCCGTCATAA CCAGAGCGAC TACGGGCGAC GACAGATCTC CATGCACATG 
CAGGAGCAGC AGCAGATGGC AGGCATGGCA TCGCAGACTG GCACATCTCA TTCATCTCTC 
GCCACTCCTC CTTCCACTGT TCAACCTGTT CGTCAAAGCT CCATGGCTCC TCCCACGCAG 
CGCATGAAAT CACAACCCTC ACATCAGCTT TCTGTGAGCT CTGCCGCTGG TGCCCCTGCG 
TCCAAAACCA GGCCGCAGAG CGGAAACCTG CTCCAGTCCC CGGAGTCTGG CTTCGGGGGC 
CGGCAGCAGG GCCCTCGGCA GCAGCTGTCT GTCAAAGACA GCACCCCACC CGGCCTGCCG 
CATCAACAGA GCTCCACCAG GGAGAGTCAG GGATCCCAGG GCTCTCAGGG TGGAACGCCG 
CAGTCCACGC AGCAGTCCAA ATCACATCAG GAACGGCAGC AGGTCCAGCA ACAGCATCTG 
CTCAAGCCTA CCATGAGCAA ACAGGGCTCT TCACAATCTC CATCCACCCT GAACCCGCAG 
ACCCCTGCCT CTGAGCGGAC GGTCGCCTGG GTCTCCAACA TGCCCCATCT TTCTGCTGAC 
ATAGAGAGTT CCCGCATCGA CAGAGAAGAG TTCAAGCTGA AGGAGTACTC CAAGAGCATG 
GACGAGTCCC GTCTGGACCG GGTGCGCGAG TATGAGGAGG AGATCCACTC ACTGAAGGAG 
CGTCTGATGA TGTCCCACCG CAAGTTGGAA GAGTACGAGA GGAGGCTGTT AACTCAGGAG 
CAGCAGACCA ATAAAATCCT GCTGCAGTAT CAGAGCCGAC TGGACGACAG CGAGAGGAGA 
CTCCGACAGC AGCAGATGGA GAAAGACAAC CAAATTAAAG GAATTATTGA CAGACTCATG 
GCGGTGGAGG ACGAGTTGAG AGTAGGAGTG GTGCCTGAAC ACAAACCCAG AGTATTTGCG 
GATCAGGAAA AGCAGCTTTC CGCCTTGGGT TCAGCAGACC CCGGTGTGAG TTTTGGAGGA 
GAAATGACCA GCGCTTCTGG CTTTGTTGGA GTCTCCCCTT CGGACAAATC CTCCACCCGA 
TCCCCCCAGA ATGGAATCGC GTCCTTCCCG TTAGTTCCCC CTCCTCGTTC AATTCCTCAC 
GCACAACTCT GGCCGAACGG CAACCAAAAA TCTGCAATGG ATTGGAGGGC AAGTTTGGAG 
GGGGAGCAGG TCAATAACAA CGCTAATCAA ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322257.1
CDS	1448..5623
Translation	MEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQEKQLSALGSADPGVSFGGEMTSASGFVGVSPSDKSSTRSPQNGIASFPLVPPPRSIPHAQLWPNGNQKSAMDWRASLEGEQVNNNANQI

Target ORF information:

RefSeq Version	XM_021466582.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466582.1

ORF Insert Sequence:

ATGGAGATCC CACCCACTGC CCCCCCACAA CCCTTCCGAC AATCCGTGAG TAACCCACCT 
TTCCGTCCCC GGTCCCTCTC TCCATCCCTG TCCCTATCGC TGCCGTTCGC TCCATCGCCC 
CTGTCGCCCT CGCTGTCCCC GTCCCATTTA CAGTCCCAAA AGCGAATCAG CACTCCTCCG 
CTGCCTCGTT CTCTCCCTCT CTCCCCGAGC GTCTCTCTGA CTCTCCCACC CTCTGTCCCG 
CTCTCCCACT GGGCTGCTGG TGAGTGGAAG GTTGTGGGTT CTAATCCAGG GACGGGATCC 
AGTGTGGCGG CACTGCCCTC TCTTGAGAGT TTCCTCAGTA GAAGACTGAA GGGGTCCATT 
AAACGGGCCA AGAGTCAGCC CAAGCTGGAC CGAACCAGCA GCTTCCGCCA CATGATCCTC 
CCTCGCTTCC GCAGCGCCGA CCAGGAGCGG ACTCGTCTGA TGCAGAGCTT CAAAGAATCA 
CACTCCCATG AGTCTTTGCT GTCTCCCAGC AGCGCAGCCG AGGCCTTGGA TTTGACACTT 
GATGAAGACG CCATCATCAA GCCTGTTCAC TCCAGTATAT TGGGACAGGA GTACTGTTTC 
GAGGTGATAA CTGCTTCAGG AACAAAGTGT TTTGCCTGTC GGTCGGCTGC GGAGCGAGAC 
AAATGGATCG AGAATCTACA GAGAGCTGTC AAACCCAACA AAGACAACAG TCGGCGTGTC 
GACAACGTCC TGAAGCTGTG GATCATTGAG GCCCGTGAGC TCCCACCCAA AAAGCGCTAT 
TATTGTGAAC TGTGCTTGGA TGACATGCTG TACGCTCGCA CCACCAGCAA ACCCCGTACC 
GACACTGTCT TCTGGGGTGA GCACTTTGAG TTCAACAACC TGCCTGCTGT CCGCAACCTC 
CGCCTTCATC TGTACAAAGA GACGGACAAG AAGAGACGCA AGGAGAAGAG CACGTACCTT 
GGCTTGGTCA GCATTCCCAT TTCCAGCATC ACTGGCCGTC AGTTTGTGGA GCAATGGTAC 
CCCGTCATTC AGCCCAGTGT TCTGGCCAAG GGTGGCGGCG TCGGAGGAGG GAAGATCATC 
AATGCCTCGC TGCGGCTCAA GTCACGCTTT CAGACCATGA GCATCCTGCC GATGGAACTG 
TATAAAGAGT TCGCAGAGTA CGTCACAAAC AACTACCGGA CGCTGTGTGC GGTCCTGGAG 
CCACTGCTGA GTGTCAAGAG CAAAGAAGAA GTGGCCTGTG CTCTGGTGCA CATCCTACAG 
AGCACAGGGA AGGCCAAGGA TTTTCTGTCA GACATGGCGA TGTGTGAGGT GGATCGCTTC 
ATCGACCGCG AGCACCTGAT CTTCAGAGAA AACACCCTGG CCACCAAAGC CATAGAAGAG 
TACCTCAAAC TCATCGGACA CAAGTACCTC AAAGATGTTT TAGGTGATTT TATCCGAGCG 
CTGTATGAAT CAGAGGAGAA CTGTGAAGTG GACCCGATGC GAACTCCCCC TTCAATACTG 
CCCGAACATC AAGCCAACCT CCGGATGTGC TGTGAGCTCG CCCTCTGCAA AATCATCAAC 
TCATATTGTG CGTTCCCGCG AGAGCTGAAG GAAGTGTTTG CATCATGGCG TGTCCGCTGT 
GCTGAGAGAG GAAGAGAAGA CATTGCAGAT CGACTGATCA GCGGCTCCCT CTTCCTGCGC 
TTCCTCTGTC CAGCCATCAT GTCTCCATCT CTGTTCAACC TCACGCAGGA ATATCCCGAT 
GAGCAGACCT CACGAACACT CACACTCATC GCCAAAGTGG TCCAGAACCT CGCAAACTTC 
TCCAAGTTTG GCAGTAAGGA GGAGTACATG TGCTTCATGA ATGAGTTTCT GGAGATGGAG 
TGGGGCTCCA TGCAGCAGTT TCTCTATGAG ATCTCCAATC TGGACAGTGT GAGCAATGCT 
GGGGCCTTCG AGGGCTACAT TGACCTAGGC CGCGAGCTCT CCATCCTGCA CAGTCTGCTC 
TGGGAGGTTA TGGCTCAGCT GAGCAAGGAT GCCATCATCA AACTTGGACC ATTGCCCCGC 
CTCCTCAATG ACATCAGCAT GGCCCTCCGC AACCCTCACC TGCAGAGGCA GCCCAGTCAT 
CAGACAGACA GACAGCCTGC AGAGAGACAG ACAGACCGAC TGCTGTCCCG ACCCAGCTTC 
AACAGGGGCG TCTCGTCTGA GTTCCAGAAT CTCATGATGA GAGACCTTAA CAGTTCAGTG 
GAGATCACTC GTCTGCCCTC TCCAACTTCT GCCATGTCCA GCGGTGGAGC TCCTCCATCC 
CGTGCAGGAA TGGCTGGGTT TGCGGACCGC GATCATCCTC ACCGAGCCTC GTCTAAAGAT 
GTTTTCTATG GTGCCCGTCC CCCACTGGCC CGCTCCAGTC CAGCGTACTG TACTAGCAGT 
TCAGATATTA CTGATCCAGA CCCAAAGGAT TCCAGAATGA ACAGCGTCTC TAACCTGCAG 
TCTATGGGGG ACATGCTCAA CTCTTCCCAG GCCTCGATTG CTGGCCTCGG CAGCTACGGT 
GGGCTCGCAG GCTTGGGTGG AGGCCTCGGA GGTCAGCTGC GAGCCGGAGG ACGTATGTCG 
GCCGGATCTG GCGGTTCCAG TATGTCCGGG GGTCTGAGGT TGAGCCAGCT GAGCCAGATG 
GGAACCACTA CCGACTCCCT GTCCCAGCAG CAGCAGCAGC AGGCCGCCGC CCTCCGCTTT 
CCCCTGTCCT TCCAGAACCC TCTGTTCCAC CTCGCTGCTG ACGGACCGCA GCTTCACCAC 
CAGCACAGTC GAGCTCAGCC TCCTCCTCCG CTCCTGCTGG CCCCAGAGCC GGACGGTTCT 
CACCCTACTT ATATCCCACA GTTTGCACAT GGTGGTTTCT CCCGAAGTGA GGACCTCTCC 
ACCCTCAGAC CCGGCCCTCA TCTGGGCCAG CCAAGTATTA TTCATTCTCA CAGCTACAGC 
GACGACTACA GCCGTCATAA CCAGAGCGAC TACGGGCGAC GACAGATCTC CATGCACATG 
CAGGAGCAGC AGCAGATGGC AGGCATGGCA TCGCAGACTG GCACATCTCA TTCATCTCTC 
GCCACTCCTC CTTCCACTGT TCAACCTGTT CGTCAAAGCT CCATGGCTCC TCCCACGCAG 
CGCATGAAAT CACAACCCTC ACATCAGCTT TCTGTGAGCT CTGCCGCTGG TGCCCCTGCG 
TCCAAAACCA GGCCGCAGAG CGGAAACCTG CTCCAGTCCC CGGAGTCTGG CTTCGGGGGC 
CGGCAGCAGG GCCCTCGGCA GCAGCTGTCT GTCAAAGACA GCACCCCACC CGGCCTGCCG 
CATCAACAGA GCTCCACCAG GGAGAGTCAG GGATCCCAGG GCTCTCAGGG TGGAACGCCG 
CAGTCCACGC AGCAGTCCAA ATCACATCAG GAACGGCAGC AGGTCCAGCA ACAGCATCTG 
CTCAAGCCTA CCATGAGCAA ACAGGGCTCT TCACAATCTC CATCCACCCT GAACCCGCAG 
ACCCCTGCCT CTGAGCGGAC GGTCGCCTGG GTCTCCAACA TGCCCCATCT TTCTGCTGAC 
ATAGAGAGTT CCCGCATCGA CAGAGAAGAG TTCAAGCTGA AGGAGTACTC CAAGAGCATG 
GACGAGTCCC GTCTGGACCG GGTGCGCGAG TATGAGGAGG AGATCCACTC ACTGAAGGAG 
CGTCTGATGA TGTCCCACCG CAAGTTGGAA GAGTACGAGA GGAGGCTGTT AACTCAGGAG 
CAGCAGACCA ATAAAATCCT GCTGCAGTAT CAGAGCCGAC TGGACGACAG CGAGAGGAGA 
CTCCGACAGC AGCAGATGGA GAAAGACAAC CAAATTAAAG GAATTATTGA CAGACTCATG 
GCGGTGGAGG ACGAGTTGAG AGTAGGAGTG GTGCCTGAAC ACAAACCCAG AGTATTTGCG 
GATCAGGAAA AGCAGCTTTC CGCCTTGGGT TCAGCAGACC CCGGTGTGAG TTTTGGAGGA 
GAAATGACCA GCGCTTCTGG CTTTGTTGGA GTCTCCCCTT CGGACAAATC CTCCACCCGA 
TCCCCCCAGA ATGGAATCGC GTCCTTCCCG TTAGTTCCCC CTCCTCGTTC AATTCCTCAC 
GCACAACTCT GGCCGAACGG CAACCAAAAA TCTGCAATGG ATTGGAGGGC AAGTTTGGAG 
GGGGAGCAGG TCAATAACAA CGCTAATCAA ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_021466583.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322258.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa149176
Clone ID Related Accession (Same CDS sequence)	XM_021466583.1
Accession Version	XM_021466583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGACT CATGGCGGTG 
GAGGACGAGT TGAGAGTAGG AGTGGTGCCT GAACACAAAC CCAGAGTATT TGCGGATCAG 
GGGAGGCGGC AGTCCATCCT AGTGCAGCCC CGCCTTGCGC CCGTCCCACT GCGAATGCAC 
AGACTTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322258.1
CDS	127..4278
Translation	MLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQGRRQSILVQPRLAPVPLRMHRLH

Target ORF information:

RefSeq Version	XM_021466583.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466583.1

ORF Insert Sequence:

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGACT CATGGCGGTG 
GAGGACGAGT TGAGAGTAGG AGTGGTGCCT GAACACAAAC CCAGAGTATT TGCGGATCAG 
GGGAGGCGGC AGTCCATCCT AGTGCAGCCC CGCCTTGCGC CCGTCCCACT GCGAATGCAC 
AGACTTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_021466585.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322260.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X9, mRNA.

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CloneID	ODa149177
Clone ID Related Accession (Same CDS sequence)	XM_021466585.1 , XM_021466584.1
Accession Version	XM_021466585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGACT CATGGCGGTG 
GAGGACGAGT TGAGAGTAGG AGTGGTGCCT GAACACAAAC CCAGAGTATT TGCGGATCAG 
ACTTCACTGA AAAGGACTGA TCATGATGAA ACACACACTG AACATGATTT AGATATAAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322260.1
CDS	127..4269
Translation	MLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQTSLKRTDHDETHTEHDLDIK

Target ORF information:

RefSeq Version	XM_021466585.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466585.1

ORF Insert Sequence:

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGACT CATGGCGGTG 
GAGGACGAGT TGAGAGTAGG AGTGGTGCCT GAACACAAAC CCAGAGTATT TGCGGATCAG 
ACTTCACTGA AAAGGACTGA TCATGATGAA ACACACACTG AACATGATTT AGATATAAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_021466584.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322259.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa149177
Clone ID Related Accession (Same CDS sequence)	XM_021466585.1 , XM_021466584.1
Accession Version	XM_021466584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGACT CATGGCGGTG 
GAGGACGAGT TGAGAGTAGG AGTGGTGCCT GAACACAAAC CCAGAGTATT TGCGGATCAG 
ACTTCACTGA AAAGGACTGA TCATGATGAA ACACACACTG AACATGATTT AGATATAAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322259.1
CDS	127..4269
Translation	MLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQTSLKRTDHDETHTEHDLDIK

Target ORF information:

RefSeq Version	XM_021466584.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466584.1

ORF Insert Sequence:

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGACT CATGGCGGTG 
GAGGACGAGT TGAGAGTAGG AGTGGTGCCT GAACACAAAC CCAGAGTATT TGCGGATCAG 
ACTTCACTGA AAAGGACTGA TCATGATGAA ACACACACTG AACATGATTT AGATATAAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_021466586.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322261.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa149178
Clone ID Related Accession (Same CDS sequence)	XM_021466586.1
Accession Version	XM_021466586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGACT CATGGCGGTG 
GAGGACGAGT TGAGAGTAGG AGTGGTGCCT GAACACAAAC CCAGAGTATT TGCGGATCAG 
CCAACAACAA AGCTTCATGG AGGACAATCT GGAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322261.1
CDS	127..4245
Translation	MLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQPTTKLHGGQSGT

Target ORF information:

RefSeq Version	XM_021466586.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466586.1

ORF Insert Sequence:

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGACT CATGGCGGTG 
GAGGACGAGT TGAGAGTAGG AGTGGTGCCT GAACACAAAC CCAGAGTATT TGCGGATCAG 
CCAACAACAA AGCTTCATGG AGGACAATCT GGAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_021466587.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322262.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X11, mRNA.

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CloneID	ODa149179
Clone ID Related Accession (Same CDS sequence)	XM_021466587.1
Accession Version	XM_021466587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCA GTTTCCTCAG TAGAAGACTG 
AAGGGGTCCA TTAAACGGGC CAAGAGTCAG CCCAAGCTGG ACCGAACCAG CAGCTTCCGC 
CACATGATCC TCCCTCGCTT CCGCAGCGCC GACCAGGAGC GGACTCGTCT GATGCAGAGC 
TTCAAAGAAT CACACTCCCA TGAGTCTTTG CTGTCTCCCA GCAGCGCAGC CGAGGCCTTG 
GATTTGACAC TTGATGAAGA CGCCATCATC AAGCCTGTTC ACTCCAGTAT ATTGGGACAG 
GAGTACTGTT TCGAGGTGAT AACTGCTTCA GGAACAAAGT GTTTTGCCTG TCGGTCGGCT 
GCGGAGCGAG ACAAATGGAT CGAGAATCTA CAGAGAGCTG TCAAACCCAA CAAAGACAAC 
AGTCGGCGTG TCGACAACGT CCTGAAGCTG TGGATCATTG AGGCCCGTGA GCTCCCACCC 
AAAAAGCGCT ATTATTGTGA ACTGTGCTTG GATGACATGC TGTACGCTCG CACCACCAGC 
AAACCCCGTA CCGACACTGT CTTCTGGGGT GAGCACTTTG AGTTCAACAA CCTGCCTGCT 
GTCCGCAACC TCCGCCTTCA TCTGTACAAA GAGACGGACA AGAAGAGACG CAAGGAGAAG 
AGCACGTACC TTGGCTTGGT CAGCATTCCC ATTTCCAGCA TCACTGGCCG TCAGTTTGTG 
GAGCAATGGT ACCCCGTCAT TCAGCCCAGT GTTCTGGCCA AGGGTGGCGG CGTCGGAGGA 
GGGAAGATCA TCAATGCCTC GCTGCGGCTC AAGTCACGCT TTCAGACCAT GAGCATCCTG 
CCGATGGAAC TGTATAAAGA GTTCGCAGAG TACGTCACAA ACAACTACCG GACGCTGTGT 
GCGGTCCTGG AGCCACTGCT GAGTGTCAAG AGCAAAGAAG AAGTGGCCTG TGCTCTGGTG 
CACATCCTAC AGAGCACAGG GAAGGCCAAG GATTTTCTGT CAGACATGGC GATGTGTGAG 
GTGGATCGCT TCATCGACCG CGAGCACCTG ATCTTCAGAG AAAACACCCT GGCCACCAAA 
GCCATAGAAG AGTACCTCAA ACTCATCGGA CACAAGTACC TCAAAGATGT TTTAGGTGAT 
TTTATCCGAG CGCTGTATGA ATCAGAGGAG AACTGTGAAG TGGACCCGAT GCGAACTCCC 
CCTTCAATAC TGCCCGAACA TCAAGCCAAC CTCCGGATGT GCTGTGAGCT CGCCCTCTGC 
AAAATCATCA ACTCATATTG TGCGTTCCCG CGAGAGCTGA AGGAAGTGTT TGCATCATGG 
CGTGTCCGCT GTGCTGAGAG AGGAAGAGAA GACATTGCAG ATCGACTGAT CAGCGGCTCC 
CTCTTCCTGC GCTTCCTCTG TCCAGCCATC ATGTCTCCAT CTCTGTTCAA CCTCACGCAG 
GAATATCCCG ATGAGCAGAC CTCACGAACA CTCACACTCA TCGCCAAAGT GGTCCAGAAC 
CTCGCAAACT TCTCCAAGTT TGGCAGTAAG GAGGAGTACA TGTGCTTCAT GAATGAGTTT 
CTGGAGATGG AGTGGGGCTC CATGCAGCAG TTTCTCTATG AGATCTCCAA TCTGGACAGT 
GTGAGCAATG CTGGGGCCTT CGAGGGCTAC ATTGACCTAG GCCGCGAGCT CTCCATCCTG 
CACAGTCTGC TCTGGGAGGT TATGGCTCAG CTGAGCAAGG ATGCCATCAT CAAACTTGGA 
CCATTGCCCC GCCTCCTCAA TGACATCAGC ATGGCCCTCC GCAACCCTCA CCTGCAGAGG 
CAGCCCAGTC ATCAGACAGA CAGACAGCCT GCAGAGAGAC AGACAGACCG ACTGCTGTCC 
CGACCCAGCT TCAACAGGGG CGTCTCGTCT GAGTTCCAGA ATCTCATGAT GAGAGACCTT 
AACAGTTCAG TGGAGATCAC TCGTCTGCCC TCTCCAACTT CTGCCATGTC CAGCGGTGGA 
GCTCCTCCAT CCCGTGCAGG AATGGCTGGG TTTGCGGACC GCGATCATCC TCACCGAGCC 
TCGTCTAAAG ATGTTTTCTA TGGTGCCCGT CCCCCACTGG CCCGCTCCAG TCCAGCGTAC 
TGTACTAGCA GTTCAGATAT TACTGATCCA GACCCAAAGG ATTCCAGAAT GAACAGCGTC 
TCTAACCTGC AGTCTATGGG GGACATGCTC AACTCTTCCC AGGCCTCGAT TGCTGGCCTC 
GGCAGCTACG GTGGGCTCGC AGGCTTGGGT GGAGGCCTCG GAGGTCAGCT GCGAGCCGGA 
GGACGTATGT CGGCCGGATC TGGCGGTTCC AGTATGTCCG GGGGTCTGAG GTTGAGCCAG 
CTGAGCCAGA TGGGAACCAC TACCGACTCC CTGTCCCAGC AGCAGCAGCA GCAGGCCGCC 
GCCCTCCGCT TTCCCCTGTC CTTCCAGAAC CCTCTGTTCC ACCTCGCTGC TGACGGACCG 
CAGCTTCACC ACCAGCACAG TCGAGCTCAG CCTCCTCCTC CGCTCCTGCT GGCCCCAGAG 
CCGGACGGTT CTCACCCTAC TTATATCCCA CAGTTTGCAC ATGGTGGTTT CTCCCGAAGT 
GAGGACCTCT CCACCCTCAG ACCCGGCCCT CATCTGGGCC AGCCAAGTAT TATTCATTCT 
CACAGCTACA GCGACGACTA CAGCCGTCAT AACCAGAGCG ACTACGGGCG ACGACAGATC 
TCCATGCACA TGCAGGAGCA GCAGCAGATG GCAGGCATGG CATCGCAGAC TGGCACATCT 
CATTCATCTC TCGCCACTCC TCCTTCCACT GTTCAACCTG TTCGTCAAAG CTCCATGGCT 
CCTCCCACGC AGCGCATGAA ATCACAACCC TCACATCAGC TTTCTGTGAG CTCTGCCGCT 
GGTGCCCCTG CGTCCAAAAC CAGGCCGCAG AGCGGAAACC TGCTCCAGTC CCCGGAGTCT 
GGCTTCGGGG GCCGGCAGCA GGGCCCTCGG CAGCAGCTGT CTGTCAAAGA CAGCACCCCA 
CCCGGCCTGC CGCATCAACA GAGCTCCACC AGGGAGAGTC AGGGATCCCA GGGCTCTCAG 
GGTGGAACGC CGCAGTCCAC GCAGCAGTCC AAATCACATC AGGAACGGCA GCAGGTCCAG 
CAACAGCATC TGCTCAAGCC TACCATGAGC AAACAGGGCT CTTCACAATC TCCATCCACC 
CTGAACCCGC AGACCCCTGC CTCTGAGCGG ACGGTCGCCT GGGTCTCCAA CATGCCCCAT 
CTTTCTGCTG ACATAGAGAG TTCCCGCATC GACAGAGAAG AGTTCAAGCT GAAGGAGTAC 
TCCAAGAGCA TGGACGAGTC CCGTCTGGAC CGGGTGCGCG AGTATGAGGA GGAGATCCAC 
TCACTGAAGG AGCGTCTGAT GATGTCCCAC CGCAAGTTGG AAGAGTACGA GAGGAGGCTG 
TTAACTCAGG AGCAGCAGAC CAATAAAATC CTGCTGCAGT ATCAGAGCCG ACTGGACGAC 
AGCGAGAGGA GACTCCGACA GCAGCAGATG GAGAAAGACA ACCAAATTAA AGGAATTATT 
GACAGACTCA TGGCGGTGGA GGACGAGTTG AGAGTAGGAG TGGTGCCTGA ACACAAACCC 
AGAGTATTTG CGGATCAGGA AAAGCAGCTT TCCGCCTTGG GTTCAGCAGA CCCCGGTGTG 
AGTTTTGGAG GAGAAATGAC CAGCGCTTCT GGCTTTGTTG GAGTCTCCCC TTCGGACAAA 
TCCTCCACCC GATCCCCCCA GAATGGAATC GCGTCCTTCC CGTTAGTTCC CCCTCCTCGT 
TCAATTCCTC ACGCACAACT CTGGCCGAAC GGCAACCAAA AATCTGCAAT GGATTGGAGG 
GCAAGTTTGG AGGGGGAGCA GGTCAATAAC AACGCTAATC AAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322262.1
CDS	127..4194
Translation	MLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSSFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQEKQLSALGSADPGVSFGGEMTSASGFVGVSPSDKSSTRSPQNGIASFPLVPPPRSIPHAQLWPNGNQKSAMDWRASLEGEQVNNNANQI

Target ORF information:

RefSeq Version	XM_021466587.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466587.1

ORF Insert Sequence:

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCA GTTTCCTCAG TAGAAGACTG 
AAGGGGTCCA TTAAACGGGC CAAGAGTCAG CCCAAGCTGG ACCGAACCAG CAGCTTCCGC 
CACATGATCC TCCCTCGCTT CCGCAGCGCC GACCAGGAGC GGACTCGTCT GATGCAGAGC 
TTCAAAGAAT CACACTCCCA TGAGTCTTTG CTGTCTCCCA GCAGCGCAGC CGAGGCCTTG 
GATTTGACAC TTGATGAAGA CGCCATCATC AAGCCTGTTC ACTCCAGTAT ATTGGGACAG 
GAGTACTGTT TCGAGGTGAT AACTGCTTCA GGAACAAAGT GTTTTGCCTG TCGGTCGGCT 
GCGGAGCGAG ACAAATGGAT CGAGAATCTA CAGAGAGCTG TCAAACCCAA CAAAGACAAC 
AGTCGGCGTG TCGACAACGT CCTGAAGCTG TGGATCATTG AGGCCCGTGA GCTCCCACCC 
AAAAAGCGCT ATTATTGTGA ACTGTGCTTG GATGACATGC TGTACGCTCG CACCACCAGC 
AAACCCCGTA CCGACACTGT CTTCTGGGGT GAGCACTTTG AGTTCAACAA CCTGCCTGCT 
GTCCGCAACC TCCGCCTTCA TCTGTACAAA GAGACGGACA AGAAGAGACG CAAGGAGAAG 
AGCACGTACC TTGGCTTGGT CAGCATTCCC ATTTCCAGCA TCACTGGCCG TCAGTTTGTG 
GAGCAATGGT ACCCCGTCAT TCAGCCCAGT GTTCTGGCCA AGGGTGGCGG CGTCGGAGGA 
GGGAAGATCA TCAATGCCTC GCTGCGGCTC AAGTCACGCT TTCAGACCAT GAGCATCCTG 
CCGATGGAAC TGTATAAAGA GTTCGCAGAG TACGTCACAA ACAACTACCG GACGCTGTGT 
GCGGTCCTGG AGCCACTGCT GAGTGTCAAG AGCAAAGAAG AAGTGGCCTG TGCTCTGGTG 
CACATCCTAC AGAGCACAGG GAAGGCCAAG GATTTTCTGT CAGACATGGC GATGTGTGAG 
GTGGATCGCT TCATCGACCG CGAGCACCTG ATCTTCAGAG AAAACACCCT GGCCACCAAA 
GCCATAGAAG AGTACCTCAA ACTCATCGGA CACAAGTACC TCAAAGATGT TTTAGGTGAT 
TTTATCCGAG CGCTGTATGA ATCAGAGGAG AACTGTGAAG TGGACCCGAT GCGAACTCCC 
CCTTCAATAC TGCCCGAACA TCAAGCCAAC CTCCGGATGT GCTGTGAGCT CGCCCTCTGC 
AAAATCATCA ACTCATATTG TGCGTTCCCG CGAGAGCTGA AGGAAGTGTT TGCATCATGG 
CGTGTCCGCT GTGCTGAGAG AGGAAGAGAA GACATTGCAG ATCGACTGAT CAGCGGCTCC 
CTCTTCCTGC GCTTCCTCTG TCCAGCCATC ATGTCTCCAT CTCTGTTCAA CCTCACGCAG 
GAATATCCCG ATGAGCAGAC CTCACGAACA CTCACACTCA TCGCCAAAGT GGTCCAGAAC 
CTCGCAAACT TCTCCAAGTT TGGCAGTAAG GAGGAGTACA TGTGCTTCAT GAATGAGTTT 
CTGGAGATGG AGTGGGGCTC CATGCAGCAG TTTCTCTATG AGATCTCCAA TCTGGACAGT 
GTGAGCAATG CTGGGGCCTT CGAGGGCTAC ATTGACCTAG GCCGCGAGCT CTCCATCCTG 
CACAGTCTGC TCTGGGAGGT TATGGCTCAG CTGAGCAAGG ATGCCATCAT CAAACTTGGA 
CCATTGCCCC GCCTCCTCAA TGACATCAGC ATGGCCCTCC GCAACCCTCA CCTGCAGAGG 
CAGCCCAGTC ATCAGACAGA CAGACAGCCT GCAGAGAGAC AGACAGACCG ACTGCTGTCC 
CGACCCAGCT TCAACAGGGG CGTCTCGTCT GAGTTCCAGA ATCTCATGAT GAGAGACCTT 
AACAGTTCAG TGGAGATCAC TCGTCTGCCC TCTCCAACTT CTGCCATGTC CAGCGGTGGA 
GCTCCTCCAT CCCGTGCAGG AATGGCTGGG TTTGCGGACC GCGATCATCC TCACCGAGCC 
TCGTCTAAAG ATGTTTTCTA TGGTGCCCGT CCCCCACTGG CCCGCTCCAG TCCAGCGTAC 
TGTACTAGCA GTTCAGATAT TACTGATCCA GACCCAAAGG ATTCCAGAAT GAACAGCGTC 
TCTAACCTGC AGTCTATGGG GGACATGCTC AACTCTTCCC AGGCCTCGAT TGCTGGCCTC 
GGCAGCTACG GTGGGCTCGC AGGCTTGGGT GGAGGCCTCG GAGGTCAGCT GCGAGCCGGA 
GGACGTATGT CGGCCGGATC TGGCGGTTCC AGTATGTCCG GGGGTCTGAG GTTGAGCCAG 
CTGAGCCAGA TGGGAACCAC TACCGACTCC CTGTCCCAGC AGCAGCAGCA GCAGGCCGCC 
GCCCTCCGCT TTCCCCTGTC CTTCCAGAAC CCTCTGTTCC ACCTCGCTGC TGACGGACCG 
CAGCTTCACC ACCAGCACAG TCGAGCTCAG CCTCCTCCTC CGCTCCTGCT GGCCCCAGAG 
CCGGACGGTT CTCACCCTAC TTATATCCCA CAGTTTGCAC ATGGTGGTTT CTCCCGAAGT 
GAGGACCTCT CCACCCTCAG ACCCGGCCCT CATCTGGGCC AGCCAAGTAT TATTCATTCT 
CACAGCTACA GCGACGACTA CAGCCGTCAT AACCAGAGCG ACTACGGGCG ACGACAGATC 
TCCATGCACA TGCAGGAGCA GCAGCAGATG GCAGGCATGG CATCGCAGAC TGGCACATCT 
CATTCATCTC TCGCCACTCC TCCTTCCACT GTTCAACCTG TTCGTCAAAG CTCCATGGCT 
CCTCCCACGC AGCGCATGAA ATCACAACCC TCACATCAGC TTTCTGTGAG CTCTGCCGCT 
GGTGCCCCTG CGTCCAAAAC CAGGCCGCAG AGCGGAAACC TGCTCCAGTC CCCGGAGTCT 
GGCTTCGGGG GCCGGCAGCA GGGCCCTCGG CAGCAGCTGT CTGTCAAAGA CAGCACCCCA 
CCCGGCCTGC CGCATCAACA GAGCTCCACC AGGGAGAGTC AGGGATCCCA GGGCTCTCAG 
GGTGGAACGC CGCAGTCCAC GCAGCAGTCC AAATCACATC AGGAACGGCA GCAGGTCCAG 
CAACAGCATC TGCTCAAGCC TACCATGAGC AAACAGGGCT CTTCACAATC TCCATCCACC 
CTGAACCCGC AGACCCCTGC CTCTGAGCGG ACGGTCGCCT GGGTCTCCAA CATGCCCCAT 
CTTTCTGCTG ACATAGAGAG TTCCCGCATC GACAGAGAAG AGTTCAAGCT GAAGGAGTAC 
TCCAAGAGCA TGGACGAGTC CCGTCTGGAC CGGGTGCGCG AGTATGAGGA GGAGATCCAC 
TCACTGAAGG AGCGTCTGAT GATGTCCCAC CGCAAGTTGG AAGAGTACGA GAGGAGGCTG 
TTAACTCAGG AGCAGCAGAC CAATAAAATC CTGCTGCAGT ATCAGAGCCG ACTGGACGAC 
AGCGAGAGGA GACTCCGACA GCAGCAGATG GAGAAAGACA ACCAAATTAA AGGAATTATT 
GACAGACTCA TGGCGGTGGA GGACGAGTTG AGAGTAGGAG TGGTGCCTGA ACACAAACCC 
AGAGTATTTG CGGATCAGGA AAAGCAGCTT TCCGCCTTGG GTTCAGCAGA CCCCGGTGTG 
AGTTTTGGAG GAGAAATGAC CAGCGCTTCT GGCTTTGTTG GAGTCTCCCC TTCGGACAAA 
TCCTCCACCC GATCCCCCCA GAATGGAATC GCGTCCTTCC CGTTAGTTCC CCCTCCTCGT 
TCAATTCCTC ACGCACAACT CTGGCCGAAC GGCAACCAAA AATCTGCAAT GGATTGGAGG 
GCAAGTTTGG AGGGGGAGCA GGTCAATAAC AACGCTAATC AAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_021466588.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322263.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X12, mRNA.

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CloneID	ODa149180
Clone ID Related Accession (Same CDS sequence)	XM_021466588.1
Accession Version	XM_021466588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGGAA TGAAGAGATG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322263.1
CDS	127..4152
Translation	MLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRNEEME

Target ORF information:

RefSeq Version	XM_021466588.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466588.1

ORF Insert Sequence:

ATGTTGGACC AGGAGGAGGT TCACCCTTTG TTGATGCGCG AGCGGCGCTC CGAGTCGCAC 
AGGAACAAGC TGCTGCGACG GACGGTCAGT GTGCCTGTGG AGGCCAGGCA CCATCCTGAA 
ATGGACGCCG CTCGGCTCCG GCGGAAAAGC ATCGCCACCG GGAAGCAGCC AAGCATGGAG 
ATCCCACCCA CTGCCCCCCC ACAACCCTTC CGACAATCCG TGAGTAACCC ACCTTTCCGT 
CCCCGGTCCC TCTCTCCATC CCTGTCCCTA TCGCTGCCGT TCGCTCCATC GCCCCTGTCG 
CCCTCGCTGT CCCCGTCCCA TTTACAGTCC CAAAAGCGAA TCAGCACTCC TCCGCTGCCT 
CGTTCTCTCC CTCTCTCCCC GAGCGTCTCT CTGACTCTCC CACCCTCTGT CCCGCTCTCC 
CACTGGGCTG CTGGTGAGTG GAAGGTTGTG GGTTCTAATC CAGGGACGGG ATCCAGTGTG 
GCGGCACTGC CCTCTCTTGA GAGTTTCCTC AGTAGAAGAC TGAAGGGGTC CATTAAACGG 
GCCAAGAGTC AGCCCAAGCT GGACCGAACC AGCAGCTTCC GCCACATGAT CCTCCCTCGC 
TTCCGCAGCG CCGACCAGGA GCGGACTCGT CTGATGCAGA GCTTCAAAGA ATCACACTCC 
CATGAGTCTT TGCTGTCTCC CAGCAGCGCA GCCGAGGCCT TGGATTTGAC ACTTGATGAA 
GACGCCATCA TCAAGCCTGT TCACTCCAGT ATATTGGGAC AGGAGTACTG TTTCGAGGTG 
ATAACTGCTT CAGGAACAAA GTGTTTTGCC TGTCGGTCGG CTGCGGAGCG AGACAAATGG 
ATCGAGAATC TACAGAGAGC TGTCAAACCC AACAAAGACA ACAGTCGGCG TGTCGACAAC 
GTCCTGAAGC TGTGGATCAT TGAGGCCCGT GAGCTCCCAC CCAAAAAGCG CTATTATTGT 
GAACTGTGCT TGGATGACAT GCTGTACGCT CGCACCACCA GCAAACCCCG TACCGACACT 
GTCTTCTGGG GTGAGCACTT TGAGTTCAAC AACCTGCCTG CTGTCCGCAA CCTCCGCCTT 
CATCTGTACA AAGAGACGGA CAAGAAGAGA CGCAAGGAGA AGAGCACGTA CCTTGGCTTG 
GTCAGCATTC CCATTTCCAG CATCACTGGC CGTCAGTTTG TGGAGCAATG GTACCCCGTC 
ATTCAGCCCA GTGTTCTGGC CAAGGGTGGC GGCGTCGGAG GAGGGAAGAT CATCAATGCC 
TCGCTGCGGC TCAAGTCACG CTTTCAGACC ATGAGCATCC TGCCGATGGA ACTGTATAAA 
GAGTTCGCAG AGTACGTCAC AAACAACTAC CGGACGCTGT GTGCGGTCCT GGAGCCACTG 
CTGAGTGTCA AGAGCAAAGA AGAAGTGGCC TGTGCTCTGG TGCACATCCT ACAGAGCACA 
GGGAAGGCCA AGGATTTTCT GTCAGACATG GCGATGTGTG AGGTGGATCG CTTCATCGAC 
CGCGAGCACC TGATCTTCAG AGAAAACACC CTGGCCACCA AAGCCATAGA AGAGTACCTC 
AAACTCATCG GACACAAGTA CCTCAAAGAT GTTTTAGGTG ATTTTATCCG AGCGCTGTAT 
GAATCAGAGG AGAACTGTGA AGTGGACCCG ATGCGAACTC CCCCTTCAAT ACTGCCCGAA 
CATCAAGCCA ACCTCCGGAT GTGCTGTGAG CTCGCCCTCT GCAAAATCAT CAACTCATAT 
TGTGCGTTCC CGCGAGAGCT GAAGGAAGTG TTTGCATCAT GGCGTGTCCG CTGTGCTGAG 
AGAGGAAGAG AAGACATTGC AGATCGACTG ATCAGCGGCT CCCTCTTCCT GCGCTTCCTC 
TGTCCAGCCA TCATGTCTCC ATCTCTGTTC AACCTCACGC AGGAATATCC CGATGAGCAG 
ACCTCACGAA CACTCACACT CATCGCCAAA GTGGTCCAGA ACCTCGCAAA CTTCTCCAAG 
TTTGGCAGTA AGGAGGAGTA CATGTGCTTC ATGAATGAGT TTCTGGAGAT GGAGTGGGGC 
TCCATGCAGC AGTTTCTCTA TGAGATCTCC AATCTGGACA GTGTGAGCAA TGCTGGGGCC 
TTCGAGGGCT ACATTGACCT AGGCCGCGAG CTCTCCATCC TGCACAGTCT GCTCTGGGAG 
GTTATGGCTC AGCTGAGCAA GGATGCCATC ATCAAACTTG GACCATTGCC CCGCCTCCTC 
AATGACATCA GCATGGCCCT CCGCAACCCT CACCTGCAGA GGCAGCCCAG TCATCAGACA 
GACAGACAGC CTGCAGAGAG ACAGACAGAC CGACTGCTGT CCCGACCCAG CTTCAACAGG 
GGCGTCTCGT CTGAGTTCCA GAATCTCATG ATGAGAGACC TTAACAGTTC AGTGGAGATC 
ACTCGTCTGC CCTCTCCAAC TTCTGCCATG TCCAGCGGTG GAGCTCCTCC ATCCCGTGCA 
GGAATGGCTG GGTTTGCGGA CCGCGATCAT CCTCACCGAG CCTCGTCTAA AGATGTTTTC 
TATGGTGCCC GTCCCCCACT GGCCCGCTCC AGTCCAGCGT ACTGTACTAG CAGTTCAGAT 
ATTACTGATC CAGACCCAAA GGATTCCAGA ATGAACAGCG TCTCTAACCT GCAGTCTATG 
GGGGACATGC TCAACTCTTC CCAGGCCTCG ATTGCTGGCC TCGGCAGCTA CGGTGGGCTC 
GCAGGCTTGG GTGGAGGCCT CGGAGGTCAG CTGCGAGCCG GAGGACGTAT GTCGGCCGGA 
TCTGGCGGTT CCAGTATGTC CGGGGGTCTG AGGTTGAGCC AGCTGAGCCA GATGGGAACC 
ACTACCGACT CCCTGTCCCA GCAGCAGCAG CAGCAGGCCG CCGCCCTCCG CTTTCCCCTG 
TCCTTCCAGA ACCCTCTGTT CCACCTCGCT GCTGACGGAC CGCAGCTTCA CCACCAGCAC 
AGTCGAGCTC AGCCTCCTCC TCCGCTCCTG CTGGCCCCAG AGCCGGACGG TTCTCACCCT 
ACTTATATCC CACAGTTTGC ACATGGTGGT TTCTCCCGAA GTGAGGACCT CTCCACCCTC 
AGACCCGGCC CTCATCTGGG CCAGCCAAGT ATTATTCATT CTCACAGCTA CAGCGACGAC 
TACAGCCGTC ATAACCAGAG CGACTACGGG CGACGACAGA TCTCCATGCA CATGCAGGAG 
CAGCAGCAGA TGGCAGGCAT GGCATCGCAG ACTGGCACAT CTCATTCATC TCTCGCCACT 
CCTCCTTCCA CTGTTCAACC TGTTCGTCAA AGCTCCATGG CTCCTCCCAC GCAGCGCATG 
AAATCACAAC CCTCACATCA GCTTTCTGTG AGCTCTGCCG CTGGTGCCCC TGCGTCCAAA 
ACCAGGCCGC AGAGCGGAAA CCTGCTCCAG TCCCCGGAGT CTGGCTTCGG GGGCCGGCAG 
CAGGGCCCTC GGCAGCAGCT GTCTGTCAAA GACAGCACCC CACCCGGCCT GCCGCATCAA 
CAGAGCTCCA CCAGGGAGAG TCAGGGATCC CAGGGCTCTC AGGGTGGAAC GCCGCAGTCC 
ACGCAGCAGT CCAAATCACA TCAGGAACGG CAGCAGGTCC AGCAACAGCA TCTGCTCAAG 
CCTACCATGA GCAAACAGGG CTCTTCACAA TCTCCATCCA CCCTGAACCC GCAGACCCCT 
GCCTCTGAGC GGACGGTCGC CTGGGTCTCC AACATGCCCC ATCTTTCTGC TGACATAGAG 
AGTTCCCGCA TCGACAGAGA AGAGTTCAAG CTGAAGGAGT ACTCCAAGAG CATGGACGAG 
TCCCGTCTGG ACCGGGTGCG CGAGTATGAG GAGGAGATCC ACTCACTGAA GGAGCGTCTG 
ATGATGTCCC ACCGCAAGTT GGAAGAGTAC GAGAGGAGGC TGTTAACTCA GGAGCAGCAG 
ACCAATAAAA TCCTGCTGCA GTATCAGAGC CGACTGGACG ACAGCGAGAG GAGACTCCGA 
CAGCAGCAGA TGGAGAAAGA CAACCAAATT AAAGGAATTA TTGACAGGAA TGAAGAGATG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_021466589.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322264.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X13, mRNA.

Price & Availability↑

CloneID	ODa149181
Clone ID Related Accession (Same CDS sequence)	XM_021466589.1
Accession Version	XM_021466589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras/Rap GTPase-activating protein SynGAP1b isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATCC CACCCACTGC CCCCCCACAA CCCTTCCGAC AATCCAGTTT CCTCAGTAGA 
AGACTGAAGG GGTCCATTAA ACGGGCCAAG AGTCAGCCCA AGCTGGACCG AACCAGCAGC 
TTCCGCCACA TGATCCTCCC TCGCTTCCGC AGCGCCGACC AGGAGCGGAC TCGTCTGATG 
CAGAGCTTCA AAGAATCACA CTCCCATGAG TCTTTGCTGT CTCCCAGCAG CGCAGCCGAG 
GCCTTGGATT TGACACTTGA TGAAGACGCC ATCATCAAGC CTGTTCACTC CAGTATATTG 
GGACAGGAGT ACTGTTTCGA GGTGATAACT GCTTCAGGAA CAAAGTGTTT TGCCTGTCGG 
TCGGCTGCGG AGCGAGACAA ATGGATCGAG AATCTACAGA GAGCTGTCAA ACCCAACAAA 
GACAACAGTC GGCGTGTCGA CAACGTCCTG AAGCTGTGGA TCATTGAGGC CCGTGAGCTC 
CCACCCAAAA AGCGCTATTA TTGTGAACTG TGCTTGGATG ACATGCTGTA CGCTCGCACC 
ACCAGCAAAC CCCGTACCGA CACTGTCTTC TGGGGTGAGC ACTTTGAGTT CAACAACCTG 
CCTGCTGTCC GCAACCTCCG CCTTCATCTG TACAAAGAGA CGGACAAGAA GAGACGCAAG 
GAGAAGAGCA CGTACCTTGG CTTGGTCAGC ATTCCCATTT CCAGCATCAC TGGCCGTCAG 
TTTGTGGAGC AATGGTACCC CGTCATTCAG CCCAGTGTTC TGGCCAAGGG TGGCGGCGTC 
GGAGGAGGGA AGATCATCAA TGCCTCGCTG CGGCTCAAGT CACGCTTTCA GACCATGAGC 
ATCCTGCCGA TGGAACTGTA TAAAGAGTTC GCAGAGTACG TCACAAACAA CTACCGGACG 
CTGTGTGCGG TCCTGGAGCC ACTGCTGAGT GTCAAGAGCA AAGAAGAAGT GGCCTGTGCT 
CTGGTGCACA TCCTACAGAG CACAGGGAAG GCCAAGGATT TTCTGTCAGA CATGGCGATG 
TGTGAGGTGG ATCGCTTCAT CGACCGCGAG CACCTGATCT TCAGAGAAAA CACCCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTC ATCGGACACA AGTACCTCAA AGATGTTTTA 
GGTGATTTTA TCCGAGCGCT GTATGAATCA GAGGAGAACT GTGAAGTGGA CCCGATGCGA 
ACTCCCCCTT CAATACTGCC CGAACATCAA GCCAACCTCC GGATGTGCTG TGAGCTCGCC 
CTCTGCAAAA TCATCAACTC ATATTGTGCG TTCCCGCGAG AGCTGAAGGA AGTGTTTGCA 
TCATGGCGTG TCCGCTGTGC TGAGAGAGGA AGAGAAGACA TTGCAGATCG ACTGATCAGC 
GGCTCCCTCT TCCTGCGCTT CCTCTGTCCA GCCATCATGT CTCCATCTCT GTTCAACCTC 
ACGCAGGAAT ATCCCGATGA GCAGACCTCA CGAACACTCA CACTCATCGC CAAAGTGGTC 
CAGAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG CTTCATGAAT 
GAGTTTCTGG AGATGGAGTG GGGCTCCATG CAGCAGTTTC TCTATGAGAT CTCCAATCTG 
GACAGTGTGA GCAATGCTGG GGCCTTCGAG GGCTACATTG ACCTAGGCCG CGAGCTCTCC 
ATCCTGCACA GTCTGCTCTG GGAGGTTATG GCTCAGCTGA GCAAGGATGC CATCATCAAA 
CTTGGACCAT TGCCCCGCCT CCTCAATGAC ATCAGCATGG CCCTCCGCAA CCCTCACCTG 
CAGAGGCAGC CCAGTCATCA GACAGACAGA CAGCCTGCAG AGAGACAGAC AGACCGACTG 
CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC TCGTCTGAGT TCCAGAATCT CATGATGAGA 
GACCTTAACA GTTCAGTGGA GATCACTCGT CTGCCCTCTC CAACTTCTGC CATGTCCAGC 
GGTGGAGCTC CTCCATCCCG TGCAGGAATG GCTGGGTTTG CGGACCGCGA TCATCCTCAC 
CGAGCCTCGT CTAAAGATGT TTTCTATGGT GCCCGTCCCC CACTGGCCCG CTCCAGTCCA 
GCGTACTGTA CTAGCAGTTC AGATATTACT GATCCAGACC CAAAGGATTC CAGAATGAAC 
AGCGTCTCTA ACCTGCAGTC TATGGGGGAC ATGCTCAACT CTTCCCAGGC CTCGATTGCT 
GGCCTCGGCA GCTACGGTGG GCTCGCAGGC TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA 
GCCGGAGGAC GTATGTCGGC CGGATCTGGC GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG 
AGCCAGCTGA GCCAGATGGG AACCACTACC GACTCCCTGT CCCAGCAGCA GCAGCAGCAG 
GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC CAGAACCCTC TGTTCCACCT CGCTGCTGAC 
GGACCGCAGC TTCACCACCA GCACAGTCGA GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC 
CCAGAGCCGG ACGGTTCTCA CCCTACTTAT ATCCCACAGT TTGCACATGG TGGTTTCTCC 
CGAAGTGAGG ACCTCTCCAC CCTCAGACCC GGCCCTCATC TGGGCCAGCC AAGTATTATT 
CATTCTCACA GCTACAGCGA CGACTACAGC CGTCATAACC AGAGCGACTA CGGGCGACGA 
CAGATCTCCA TGCACATGCA GGAGCAGCAG CAGATGGCAG GCATGGCATC GCAGACTGGC 
ACATCTCATT CATCTCTCGC CACTCCTCCT TCCACTGTTC AACCTGTTCG TCAAAGCTCC 
ATGGCTCCTC CCACGCAGCG CATGAAATCA CAACCCTCAC ATCAGCTTTC TGTGAGCTCT 
GCCGCTGGTG CCCCTGCGTC CAAAACCAGG CCGCAGAGCG GAAACCTGCT CCAGTCCCCG 
GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC CCTCGGCAGC AGCTGTCTGT CAAAGACAGC 
ACCCCACCCG GCCTGCCGCA TCAACAGAGC TCCACCAGGG AGAGTCAGGG ATCCCAGGGC 
TCTCAGGGTG GAACGCCGCA GTCCACGCAG CAGTCCAAAT CACATCAGGA ACGGCAGCAG 
GTCCAGCAAC AGCATCTGCT CAAGCCTACC ATGAGCAAAC AGGGCTCTTC ACAATCTCCA 
TCCACCCTGA ACCCGCAGAC CCCTGCCTCT GAGCGGACGG TCGCCTGGGT CTCCAACATG 
CCCCATCTTT CTGCTGACAT AGAGAGTTCC CGCATCGACA GAGAAGAGTT CAAGCTGAAG 
GAGTACTCCA AGAGCATGGA CGAGTCCCGT CTGGACCGGG TGCGCGAGTA TGAGGAGGAG 
ATCCACTCAC TGAAGGAGCG TCTGATGATG TCCCACCGCA AGTTGGAAGA GTACGAGAGG 
AGGCTGTTAA CTCAGGAGCA GCAGACCAAT AAAATCCTGC TGCAGTATCA GAGCCGACTG 
GACGACAGCG AGAGGAGACT CCGACAGCAG CAGATGGAGA AAGACAACCA AATTAAAGGA 
ATTATTGACA GACTCATGGC GGTGGAGGAC GAGTTGAGAG TAGGAGTGGT GCCTGAACAC 
AAACCCAGAG TATTTGCGGA TCAGGAAAAG CAGCTTTCCG CCTTGGGTTC AGCAGACCCC 
GGTGTGAGTT TTGGAGGAGA AATGACCAGC GCTTCTGGCT TTGTTGGAGT CTCCCCTTCG 
GACAAATCCT CCACCCGATC CCCCCAGAAT GGAATCGCGT CCTTCCCGTT AGTTCCCCCT 
CCTCGTTCAA TTCCTCACGC ACAACTCTGG CCGAACGGCA ACCAAAAATC TGCAATGGAT 
TGGAGGGCAA GTTTGGAGGG GGAGCAGGTC AATAACAACG CTAATCAAAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322264.1
CDS	1447..5340
Translation	MEIPPTAPPQPFRQSSFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQEKQLSALGSADPGVSFGGEMTSASGFVGVSPSDKSSTRSPQNGIASFPLVPPPRSIPHAQLWPNGNQKSAMDWRASLEGEQVNNNANQI

Target ORF information:

RefSeq Version	XM_021466589.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466589.1

ORF Insert Sequence:

ATGGAGATCC CACCCACTGC CCCCCCACAA CCCTTCCGAC AATCCAGTTT CCTCAGTAGA 
AGACTGAAGG GGTCCATTAA ACGGGCCAAG AGTCAGCCCA AGCTGGACCG AACCAGCAGC 
TTCCGCCACA TGATCCTCCC TCGCTTCCGC AGCGCCGACC AGGAGCGGAC TCGTCTGATG 
CAGAGCTTCA AAGAATCACA CTCCCATGAG TCTTTGCTGT CTCCCAGCAG CGCAGCCGAG 
GCCTTGGATT TGACACTTGA TGAAGACGCC ATCATCAAGC CTGTTCACTC CAGTATATTG 
GGACAGGAGT ACTGTTTCGA GGTGATAACT GCTTCAGGAA CAAAGTGTTT TGCCTGTCGG 
TCGGCTGCGG AGCGAGACAA ATGGATCGAG AATCTACAGA GAGCTGTCAA ACCCAACAAA 
GACAACAGTC GGCGTGTCGA CAACGTCCTG AAGCTGTGGA TCATTGAGGC CCGTGAGCTC 
CCACCCAAAA AGCGCTATTA TTGTGAACTG TGCTTGGATG ACATGCTGTA CGCTCGCACC 
ACCAGCAAAC CCCGTACCGA CACTGTCTTC TGGGGTGAGC ACTTTGAGTT CAACAACCTG 
CCTGCTGTCC GCAACCTCCG CCTTCATCTG TACAAAGAGA CGGACAAGAA GAGACGCAAG 
GAGAAGAGCA CGTACCTTGG CTTGGTCAGC ATTCCCATTT CCAGCATCAC TGGCCGTCAG 
TTTGTGGAGC AATGGTACCC CGTCATTCAG CCCAGTGTTC TGGCCAAGGG TGGCGGCGTC 
GGAGGAGGGA AGATCATCAA TGCCTCGCTG CGGCTCAAGT CACGCTTTCA GACCATGAGC 
ATCCTGCCGA TGGAACTGTA TAAAGAGTTC GCAGAGTACG TCACAAACAA CTACCGGACG 
CTGTGTGCGG TCCTGGAGCC ACTGCTGAGT GTCAAGAGCA AAGAAGAAGT GGCCTGTGCT 
CTGGTGCACA TCCTACAGAG CACAGGGAAG GCCAAGGATT TTCTGTCAGA CATGGCGATG 
TGTGAGGTGG ATCGCTTCAT CGACCGCGAG CACCTGATCT TCAGAGAAAA CACCCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTC ATCGGACACA AGTACCTCAA AGATGTTTTA 
GGTGATTTTA TCCGAGCGCT GTATGAATCA GAGGAGAACT GTGAAGTGGA CCCGATGCGA 
ACTCCCCCTT CAATACTGCC CGAACATCAA GCCAACCTCC GGATGTGCTG TGAGCTCGCC 
CTCTGCAAAA TCATCAACTC ATATTGTGCG TTCCCGCGAG AGCTGAAGGA AGTGTTTGCA 
TCATGGCGTG TCCGCTGTGC TGAGAGAGGA AGAGAAGACA TTGCAGATCG ACTGATCAGC 
GGCTCCCTCT TCCTGCGCTT CCTCTGTCCA GCCATCATGT CTCCATCTCT GTTCAACCTC 
ACGCAGGAAT ATCCCGATGA GCAGACCTCA CGAACACTCA CACTCATCGC CAAAGTGGTC 
CAGAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG CTTCATGAAT 
GAGTTTCTGG AGATGGAGTG GGGCTCCATG CAGCAGTTTC TCTATGAGAT CTCCAATCTG 
GACAGTGTGA GCAATGCTGG GGCCTTCGAG GGCTACATTG ACCTAGGCCG CGAGCTCTCC 
ATCCTGCACA GTCTGCTCTG GGAGGTTATG GCTCAGCTGA GCAAGGATGC CATCATCAAA 
CTTGGACCAT TGCCCCGCCT CCTCAATGAC ATCAGCATGG CCCTCCGCAA CCCTCACCTG 
CAGAGGCAGC CCAGTCATCA GACAGACAGA CAGCCTGCAG AGAGACAGAC AGACCGACTG 
CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC TCGTCTGAGT TCCAGAATCT CATGATGAGA 
GACCTTAACA GTTCAGTGGA GATCACTCGT CTGCCCTCTC CAACTTCTGC CATGTCCAGC 
GGTGGAGCTC CTCCATCCCG TGCAGGAATG GCTGGGTTTG CGGACCGCGA TCATCCTCAC 
CGAGCCTCGT CTAAAGATGT TTTCTATGGT GCCCGTCCCC CACTGGCCCG CTCCAGTCCA 
GCGTACTGTA CTAGCAGTTC AGATATTACT GATCCAGACC CAAAGGATTC CAGAATGAAC 
AGCGTCTCTA ACCTGCAGTC TATGGGGGAC ATGCTCAACT CTTCCCAGGC CTCGATTGCT 
GGCCTCGGCA GCTACGGTGG GCTCGCAGGC TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA 
GCCGGAGGAC GTATGTCGGC CGGATCTGGC GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG 
AGCCAGCTGA GCCAGATGGG AACCACTACC GACTCCCTGT CCCAGCAGCA GCAGCAGCAG 
GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC CAGAACCCTC TGTTCCACCT CGCTGCTGAC 
GGACCGCAGC TTCACCACCA GCACAGTCGA GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC 
CCAGAGCCGG ACGGTTCTCA CCCTACTTAT ATCCCACAGT TTGCACATGG TGGTTTCTCC 
CGAAGTGAGG ACCTCTCCAC CCTCAGACCC GGCCCTCATC TGGGCCAGCC AAGTATTATT 
CATTCTCACA GCTACAGCGA CGACTACAGC CGTCATAACC AGAGCGACTA CGGGCGACGA 
CAGATCTCCA TGCACATGCA GGAGCAGCAG CAGATGGCAG GCATGGCATC GCAGACTGGC 
ACATCTCATT CATCTCTCGC CACTCCTCCT TCCACTGTTC AACCTGTTCG TCAAAGCTCC 
ATGGCTCCTC CCACGCAGCG CATGAAATCA CAACCCTCAC ATCAGCTTTC TGTGAGCTCT 
GCCGCTGGTG CCCCTGCGTC CAAAACCAGG CCGCAGAGCG GAAACCTGCT CCAGTCCCCG 
GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC CCTCGGCAGC AGCTGTCTGT CAAAGACAGC 
ACCCCACCCG GCCTGCCGCA TCAACAGAGC TCCACCAGGG AGAGTCAGGG ATCCCAGGGC 
TCTCAGGGTG GAACGCCGCA GTCCACGCAG CAGTCCAAAT CACATCAGGA ACGGCAGCAG 
GTCCAGCAAC AGCATCTGCT CAAGCCTACC ATGAGCAAAC AGGGCTCTTC ACAATCTCCA 
TCCACCCTGA ACCCGCAGAC CCCTGCCTCT GAGCGGACGG TCGCCTGGGT CTCCAACATG 
CCCCATCTTT CTGCTGACAT AGAGAGTTCC CGCATCGACA GAGAAGAGTT CAAGCTGAAG 
GAGTACTCCA AGAGCATGGA CGAGTCCCGT CTGGACCGGG TGCGCGAGTA TGAGGAGGAG 
ATCCACTCAC TGAAGGAGCG TCTGATGATG TCCCACCGCA AGTTGGAAGA GTACGAGAGG 
AGGCTGTTAA CTCAGGAGCA GCAGACCAAT AAAATCCTGC TGCAGTATCA GAGCCGACTG 
GACGACAGCG AGAGGAGACT CCGACAGCAG CAGATGGAGA AAGACAACCA AATTAAAGGA 
ATTATTGACA GACTCATGGC GGTGGAGGAC GAGTTGAGAG TAGGAGTGGT GCCTGAACAC 
AAACCCAGAG TATTTGCGGA TCAGGAAAAG CAGCTTTCCG CCTTGGGTTC AGCAGACCCC 
GGTGTGAGTT TTGGAGGAGA AATGACCAGC GCTTCTGGCT TTGTTGGAGT CTCCCCTTCG 
GACAAATCCT CCACCCGATC CCCCCAGAAT GGAATCGCGT CCTTCCCGTT AGTTCCCCCT 
CCTCGTTCAA TTCCTCACGC ACAACTCTGG CCGAACGGCA ACCAAAAATC TGCAATGGAT 
TGGAGGGCAA GTTTGGAGGG GGAGCAGGTC AATAACAACG CTAATCAAAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_009292642.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290917.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa48963
Clone ID Related Accession (Same CDS sequence)	XM_009292642.2
Accession Version	XM_009292642.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	si:dkey-246f6.1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292642.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGGATGCCAT CATCAAACTT 
GGACCATTGC CCCGCCTCCT CAATGACATC AGCATGGCCC TCCGCAACCC TCACCTGCAG 
AGGCAGCCCA GTCATCAGAC AGACAGACAG CCTGCAGAGA GACAGACAGA CCGACTGCTG 
TCCCGACCCA GCTTCAACAG GGGCGTCTCG TCTGAGTTCC AGAATCTCAT GATGAGAGAC 
CTTAACAGTT CAGTGGAGAT CACTCGTCTG CCCTCTCCAA CTTCTGCCAT GTCCAGCGGT 
GGAGCTCCTC CATCCCGTGC AGGAATGGCT GGGTTTGCGG ACCGCGATCA TCCTCACCGA 
GCCTCGTCTA AAGATGTTTT CTATGGTGCC CGTCCCCCAC TGGCCCGCTC CAGTCCAGCG 
TACTGTACTA GCAGTTCAGA TATTACTGAT CCAGACCCAA AGGATTCCAG AATGAACAGC 
GTCTCTAACC TGCAGTCTAT GGGGGACATG CTCAACTCTT CCCAGGCCTC GATTGCTGGC 
CTCGGCAGCT ACGGTGGGCT CGCAGGCTTG GGTGGAGGCC TCGGAGGTCA GCTGCGAGCC 
GGAGGACGTA TGTCGGCCGG ATCTGGCGGT TCCAGTATGT CCGGGGGTCT GAGGTTGAGC 
CAGCTGAGCC AGATGGGAAC CACTACCGAC TCCCTGTCCC AGCAGCAGCA GCAGCAGGCC 
GCCGCCCTCC GCTTTCCCCT GTCCTTCCAG AACCCTCTGT TCCACCTCGC TGCTGACGGA 
CCGCAGCTTC ACCACCAGCA CAGTCGAGCT CAGCCTCCTC CTCCGCTCCT GCTGGCCCCA 
GAGCCGGACG GTTCTCACCC TACTTATATC CCACAGTTTG CACATGGTGG TTTCTCCCGA 
AGTGAGGACC TCTCCACCCT CAGACCCGGC CCTCATCTGG GCCAGCCAAG TATTATTCAT 
TCTCACAGCT ACAGCGACGA CTACAGCCGT CATAACCAGA GCGACTACGG GCGACGACAG 
ATCTCCATGC ACATGCAGGA GCAGCAGCAG ATGGCAGGCA TGGCATCGCA GACTGGCACA 
TCTCATTCAT CTCTCGCCAC TCCTCCTTCC ACTGTTCAAC CTGTTCGTCA AAGCTCCATG 
GCTCCTCCCA CGCAGCGCAT GAAATCACAA CCCTCACATC AGCTTTCTGT GAGCTCTGCC 
GCTGGTGCCC CTGCGTCCAA AACCAGGCCG CAGAGCGGAA ACCTGCTCCA GTCCCCGGAG 
TCTGGCTTCG GGGGCCGGCA GCAGGGCCCT CGGCAGCAGC TGTCTGTCAA AGACAGCACC 
CCACCCGGCC TGCCGCATCA ACAGAGCTCC ACCAGGGAGA GTCAGGGATC CCAGGGCTCT 
CAGGGTGGAA CGCCGCAGTC CACGCAGCAG TCCAAATCAC ATCAGGAACG GCAGCAGGTC 
CAGCAACAGC ATCTGCTCAA GCCTACCATG AGCAAACAGG GCTCTTCACA ATCTCCATCC 
ACCCTGAACC CGCAGACCCC TGCCTCTGAG CGGACGGTCG CCTGGGTCTC CAACATGCCC 
CATCTTTCTG CTGACATAGA GAGTTCCCGC ATCGACAGAG AAGAGTTCAA GCTGAAGGAG 
TACTCCAAGA GCATGGACGA GTCCCGTCTG GACCGGGTGC GCGAGTATGA GGAGGAGATC 
CACTCACTGA AGGAGCGTCT GATGATGTCC CACCGCAAGT TGGAAGAGTA CGAGAGGAGG 
CTGTTAACTC AGGAGCAGCA GACCAATAAA ATCCTGCTGC AGTATCAGAG CCGACTGGAC 
GACAGCGAGA GGAGACTCCG ACAGCAGCAG ATGGAGAAAG ACAACCAAAT TAAAGGAATT 
ATTGACAGAC TCATGGCGGT GGAGGACGAG TTGAGAGTAG GAGTGGTGCC TGAACACAAA 
CCCAGAGTAT TTGCGGATCA GGAAAAGCAG CTTTCCGCCT TGGGTTCAGC AGACCCCGGT 
GTGAGTTTTG GAGGAGAAAT GACCAGCGCT TCTGGCTTTG TTGGAGTCTC CCCTTCGGAC 
AAATCCTCCA CCCGATCCCC CCAGAATGGA ATCGCGTCCT TCCCGTTAGT TCCCCCTCCT 
CGTTCAATTC CTCACGCACA ACTCTGGCCG AACGGCAACC AAAAATCTGC AATGGATTGG 
AGGGCAAGTT TGGAGGGGGA GCAGGTCAAT AACAACGCTA ATCAAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290917.1
CDS	316..4806
Translation	MSYVPFQDARYAVPPSYRPQPSFAAAPSYEQADWNPRLGVISGNQLYMLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQEKQLSALGSADPGVSFGGEMTSASGFVGVSPSDKSSTRSPQNGIASFPLVPPPRSIPHAQLWPNGNQKSAMDWRASLEGEQVNNNANQI

Target ORF information:

RefSeq Version	XM_009292642.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292642.2

ORF Insert Sequence:

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGGATGCCAT CATCAAACTT 
GGACCATTGC CCCGCCTCCT CAATGACATC AGCATGGCCC TCCGCAACCC TCACCTGCAG 
AGGCAGCCCA GTCATCAGAC AGACAGACAG CCTGCAGAGA GACAGACAGA CCGACTGCTG 
TCCCGACCCA GCTTCAACAG GGGCGTCTCG TCTGAGTTCC AGAATCTCAT GATGAGAGAC 
CTTAACAGTT CAGTGGAGAT CACTCGTCTG CCCTCTCCAA CTTCTGCCAT GTCCAGCGGT 
GGAGCTCCTC CATCCCGTGC AGGAATGGCT GGGTTTGCGG ACCGCGATCA TCCTCACCGA 
GCCTCGTCTA AAGATGTTTT CTATGGTGCC CGTCCCCCAC TGGCCCGCTC CAGTCCAGCG 
TACTGTACTA GCAGTTCAGA TATTACTGAT CCAGACCCAA AGGATTCCAG AATGAACAGC 
GTCTCTAACC TGCAGTCTAT GGGGGACATG CTCAACTCTT CCCAGGCCTC GATTGCTGGC 
CTCGGCAGCT ACGGTGGGCT CGCAGGCTTG GGTGGAGGCC TCGGAGGTCA GCTGCGAGCC 
GGAGGACGTA TGTCGGCCGG ATCTGGCGGT TCCAGTATGT CCGGGGGTCT GAGGTTGAGC 
CAGCTGAGCC AGATGGGAAC CACTACCGAC TCCCTGTCCC AGCAGCAGCA GCAGCAGGCC 
GCCGCCCTCC GCTTTCCCCT GTCCTTCCAG AACCCTCTGT TCCACCTCGC TGCTGACGGA 
CCGCAGCTTC ACCACCAGCA CAGTCGAGCT CAGCCTCCTC CTCCGCTCCT GCTGGCCCCA 
GAGCCGGACG GTTCTCACCC TACTTATATC CCACAGTTTG CACATGGTGG TTTCTCCCGA 
AGTGAGGACC TCTCCACCCT CAGACCCGGC CCTCATCTGG GCCAGCCAAG TATTATTCAT 
TCTCACAGCT ACAGCGACGA CTACAGCCGT CATAACCAGA GCGACTACGG GCGACGACAG 
ATCTCCATGC ACATGCAGGA GCAGCAGCAG ATGGCAGGCA TGGCATCGCA GACTGGCACA 
TCTCATTCAT CTCTCGCCAC TCCTCCTTCC ACTGTTCAAC CTGTTCGTCA AAGCTCCATG 
GCTCCTCCCA CGCAGCGCAT GAAATCACAA CCCTCACATC AGCTTTCTGT GAGCTCTGCC 
GCTGGTGCCC CTGCGTCCAA AACCAGGCCG CAGAGCGGAA ACCTGCTCCA GTCCCCGGAG 
TCTGGCTTCG GGGGCCGGCA GCAGGGCCCT CGGCAGCAGC TGTCTGTCAA AGACAGCACC 
CCACCCGGCC TGCCGCATCA ACAGAGCTCC ACCAGGGAGA GTCAGGGATC CCAGGGCTCT 
CAGGGTGGAA CGCCGCAGTC CACGCAGCAG TCCAAATCAC ATCAGGAACG GCAGCAGGTC 
CAGCAACAGC ATCTGCTCAA GCCTACCATG AGCAAACAGG GCTCTTCACA ATCTCCATCC 
ACCCTGAACC CGCAGACCCC TGCCTCTGAG CGGACGGTCG CCTGGGTCTC CAACATGCCC 
CATCTTTCTG CTGACATAGA GAGTTCCCGC ATCGACAGAG AAGAGTTCAA GCTGAAGGAG 
TACTCCAAGA GCATGGACGA GTCCCGTCTG GACCGGGTGC GCGAGTATGA GGAGGAGATC 
CACTCACTGA AGGAGCGTCT GATGATGTCC CACCGCAAGT TGGAAGAGTA CGAGAGGAGG 
CTGTTAACTC AGGAGCAGCA GACCAATAAA ATCCTGCTGC AGTATCAGAG CCGACTGGAC 
GACAGCGAGA GGAGACTCCG ACAGCAGCAG ATGGAGAAAG ACAACCAAAT TAAAGGAATT 
ATTGACAGAC TCATGGCGGT GGAGGACGAG TTGAGAGTAG GAGTGGTGCC TGAACACAAA 
CCCAGAGTAT TTGCGGATCA GGAAAAGCAG CTTTCCGCCT TGGGTTCAGC AGACCCCGGT 
GTGAGTTTTG GAGGAGAAAT GACCAGCGCT TCTGGCTTTG TTGGAGTCTC CCCTTCGGAC 
AAATCCTCCA CCCGATCCCC CCAGAATGGA ATCGCGTCCT TCCCGTTAGT TCCCCCTCCT 
CGTTCAATTC CTCACGCACA ACTCTGGCCG AACGGCAACC AAAAATCTGC AATGGATTGG 
AGGGCAAGTT TGGAGGGGGA GCAGGTCAAT AACAACGCTA ATCAAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_009292643.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290918.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X3, mRNA.

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CloneID	ODa48964
Clone ID Related Accession (Same CDS sequence)	XM_009292643.2
Accession Version	XM_009292643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	si:dkey-246f6.1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGCCTC GATTGCTGGC CTCGGCAGCT ACGGTGGGCT CGCAGGCTTG 
GGTGGAGGCC TCGGAGGTCA GCTGCGAGCC GGAGGACGTA TGTCGGCCGG ATCTGGCGGT 
TCCAGTATGT CCGGGGGTCT GAGGTTGAGC CAGCTGAGCC AGATGGGAAC CACTACCGAC 
TCCCTGTCCC AGCAGCAGCA GCAGCAGGCC GCCGCCCTCC GCTTTCCCCT GTCCTTCCAG 
AACCCTCTGT TCCACCTCGC TGCTGACGGA CCGCAGCTTC ACCACCAGCA CAGTCGAGCT 
CAGCCTCCTC CTCCGCTCCT GCTGGCCCCA GAGCCGGACG GTTCTCACCC TACTTATATC 
CCACAGTTTG CACATGGTGG TTTCTCCCGA AGTGAGGACC TCTCCACCCT CAGACCCGGC 
CCTCATCTGG GCCAGCCAAG TATTATTCAT TCTCACAGCT ACAGCGACGA CTACAGCCGT 
CATAACCAGA GCGACTACGG GCGACGACAG ATCTCCATGC ACATGCAGGA GCAGCAGCAG 
ATGGCAGGCA TGGCATCGCA GACTGGCACA TCTCATTCAT CTCTCGCCAC TCCTCCTTCC 
ACTGTTCAAC CTGTTCGTCA AAGCTCCATG GCTCCTCCCA CGCAGCGCAT GAAATCACAA 
CCCTCACATC AGCTTTCTGT GAGCTCTGCC GCTGGTGCCC CTGCGTCCAA AACCAGGCCG 
CAGAGCGGAA ACCTGCTCCA GTCCCCGGAG TCTGGCTTCG GGGGCCGGCA GCAGGGCCCT 
CGGCAGCAGC TGTCTGTCAA AGACAGCACC CCACCCGGCC TGCCGCATCA ACAGAGCTCC 
ACCAGGGAGA GTCAGGGATC CCAGGGCTCT CAGGGTGGAA CGCCGCAGTC CACGCAGCAG 
TCCAAATCAC ATCAGGAACG GCAGCAGGTC CAGCAACAGC ATCTGCTCAA GCCTACCATG 
AGCAAACAGG GCTCTTCACA ATCTCCATCC ACCCTGAACC CGCAGACCCC TGCCTCTGAG 
CGGACGGTCG CCTGGGTCTC CAACATGCCC CATCTTTCTG CTGACATAGA GAGTTCCCGC 
ATCGACAGAG AAGAGTTCAA GCTGAAGGAG TACTCCAAGA GCATGGACGA GTCCCGTCTG 
GACCGGGTGC GCGAGTATGA GGAGGAGATC CACTCACTGA AGGAGCGTCT GATGATGTCC 
CACCGCAAGT TGGAAGAGTA CGAGAGGAGG CTGTTAACTC AGGAGCAGCA GACCAATAAA 
ATCCTGCTGC AGTATCAGAG CCGACTGGAC GACAGCGAGA GGAGACTCCG ACAGCAGCAG 
ATGGAGAAAG ACAACCAAAT TAAAGGAATT ATTGACAGAC TCATGGCGGT GGAGGACGAG 
TTGAGAGTAG GAGTGGTGCC TGAACACAAA CCCAGAGTAT TTGCGGATCA GGAAAAGCAG 
CTTTCCGCCT TGGGTTCAGC AGACCCCGGT GTGAGTTTTG GAGGAGAAAT GACCAGCGCT 
TCTGGCTTTG TTGGAGTCTC CCCTTCGGAC AAATCCTCCA CCCGATCCCC CCAGAATGGA 
ATCGCGTCCT TCCCGTTAGT TCCCCCTCCT CGTTCAATTC CTCACGCACA ACTCTGGCCG 
AACGGCAACC AAAAATCTGC AATGGATTGG AGGGCAAGTT TGGAGGGGGA GCAGGTCAAT 
AACAACGCTA ATCAAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290918.1
CDS	316..4776
Translation	MSYVPFQDARYAVPPSYRPQPSFAAAPSYEQADWNPRLGVISGNQLYMLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKSSIRSWFIVSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQEKQLSALGSADPGVSFGGEMTSASGFVGVSPSDKSSTRSPQNGIASFPLVPPPRSIPHAQLWPNGNQKSAMDWRASLEGEQVNNNANQI

Target ORF information:

RefSeq Version	XM_009292643.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292643.2

ORF Insert Sequence:

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGCCTC GATTGCTGGC CTCGGCAGCT ACGGTGGGCT CGCAGGCTTG 
GGTGGAGGCC TCGGAGGTCA GCTGCGAGCC GGAGGACGTA TGTCGGCCGG ATCTGGCGGT 
TCCAGTATGT CCGGGGGTCT GAGGTTGAGC CAGCTGAGCC AGATGGGAAC CACTACCGAC 
TCCCTGTCCC AGCAGCAGCA GCAGCAGGCC GCCGCCCTCC GCTTTCCCCT GTCCTTCCAG 
AACCCTCTGT TCCACCTCGC TGCTGACGGA CCGCAGCTTC ACCACCAGCA CAGTCGAGCT 
CAGCCTCCTC CTCCGCTCCT GCTGGCCCCA GAGCCGGACG GTTCTCACCC TACTTATATC 
CCACAGTTTG CACATGGTGG TTTCTCCCGA AGTGAGGACC TCTCCACCCT CAGACCCGGC 
CCTCATCTGG GCCAGCCAAG TATTATTCAT TCTCACAGCT ACAGCGACGA CTACAGCCGT 
CATAACCAGA GCGACTACGG GCGACGACAG ATCTCCATGC ACATGCAGGA GCAGCAGCAG 
ATGGCAGGCA TGGCATCGCA GACTGGCACA TCTCATTCAT CTCTCGCCAC TCCTCCTTCC 
ACTGTTCAAC CTGTTCGTCA AAGCTCCATG GCTCCTCCCA CGCAGCGCAT GAAATCACAA 
CCCTCACATC AGCTTTCTGT GAGCTCTGCC GCTGGTGCCC CTGCGTCCAA AACCAGGCCG 
CAGAGCGGAA ACCTGCTCCA GTCCCCGGAG TCTGGCTTCG GGGGCCGGCA GCAGGGCCCT 
CGGCAGCAGC TGTCTGTCAA AGACAGCACC CCACCCGGCC TGCCGCATCA ACAGAGCTCC 
ACCAGGGAGA GTCAGGGATC CCAGGGCTCT CAGGGTGGAA CGCCGCAGTC CACGCAGCAG 
TCCAAATCAC ATCAGGAACG GCAGCAGGTC CAGCAACAGC ATCTGCTCAA GCCTACCATG 
AGCAAACAGG GCTCTTCACA ATCTCCATCC ACCCTGAACC CGCAGACCCC TGCCTCTGAG 
CGGACGGTCG CCTGGGTCTC CAACATGCCC CATCTTTCTG CTGACATAGA GAGTTCCCGC 
ATCGACAGAG AAGAGTTCAA GCTGAAGGAG TACTCCAAGA GCATGGACGA GTCCCGTCTG 
GACCGGGTGC GCGAGTATGA GGAGGAGATC CACTCACTGA AGGAGCGTCT GATGATGTCC 
CACCGCAAGT TGGAAGAGTA CGAGAGGAGG CTGTTAACTC AGGAGCAGCA GACCAATAAA 
ATCCTGCTGC AGTATCAGAG CCGACTGGAC GACAGCGAGA GGAGACTCCG ACAGCAGCAG 
ATGGAGAAAG ACAACCAAAT TAAAGGAATT ATTGACAGAC TCATGGCGGT GGAGGACGAG 
TTGAGAGTAG GAGTGGTGCC TGAACACAAA CCCAGAGTAT TTGCGGATCA GGAAAAGCAG 
CTTTCCGCCT TGGGTTCAGC AGACCCCGGT GTGAGTTTTG GAGGAGAAAT GACCAGCGCT 
TCTGGCTTTG TTGGAGTCTC CCCTTCGGAC AAATCCTCCA CCCGATCCCC CCAGAATGGA 
ATCGCGTCCT TCCCGTTAGT TCCCCCTCCT CGTTCAATTC CTCACGCACA ACTCTGGCCG 
AACGGCAACC AAAAATCTGC AATGGATTGG AGGGCAAGTT TGGAGGGGGA GCAGGTCAAT 
AACAACGCTA ATCAAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_009292644.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290919.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X4, mRNA.

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CloneID	ODa48965
Clone ID Related Accession (Same CDS sequence)	XM_009292644.2
Accession Version	XM_009292644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	si:dkey-246f6.1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292644.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGATTC CAGAATGAAC AGCGTCTCTA ACCTGCAGTC TATGGGGGAC 
ATGCTCAACT CTTCCCAGGC CTCGATTGCT GGCCTCGGCA GCTACGGTGG GCTCGCAGGC 
TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA GCCGGAGGAC GTATGTCGGC CGGATCTGGC 
GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG AGCCAGCTGA GCCAGATGGG AACCACTACC 
GACTCCCTGT CCCAGCAGCA GCAGCAGCAG GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC 
CAGAACCCTC TGTTCCACCT CGCTGCTGAC GGACCGCAGC TTCACCACCA GCACAGTCGA 
GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC CCAGAGCCGG ACGGTTCTCA CCCTACTTAT 
ATCCCACAGT TTGCACATGG TGGTTTCTCC CGAAGTGAGG ACCTCTCCAC CCTCAGACCC 
GGCCCTCATC TGGGCCAGCC AAGTATTATT CATTCTCACA GCTACAGCGA CGACTACAGC 
CGTCATAACC AGAGCGACTA CGGGCGACGA CAGATCTCCA TGCACATGCA GGAGCAGCAG 
CAGATGGCAG GCATGGCATC GCAGACTGGC ACATCTCATT CATCTCTCGC CACTCCTCCT 
TCCACTGTTC AACCTGTTCG TCAAAGCTCC ATGGCTCCTC CCACGCAGCG CATGAAATCA 
CAACCCTCAC ATCAGCTTTC TGTGAGCTCT GCCGCTGGTG CCCCTGCGTC CAAAACCAGG 
CCGCAGAGCG GAAACCTGCT CCAGTCCCCG GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC 
CCTCGGCAGC AGCTGTCTGT CAAAGACAGC ACCCCACCCG GCCTGCCGCA TCAACAGAGC 
TCCACCAGGG AGAGTCAGGG ATCCCAGGGC TCTCAGGGTG GAACGCCGCA GTCCACGCAG 
CAGTCCAAAT CACATCAGGA ACGGCAGCAG GTCCAGCAAC AGCATCTGCT CAAGCCTACC 
ATGAGCAAAC AGGGCTCTTC ACAATCTCCA TCCACCCTGA ACCCGCAGAC CCCTGCCTCT 
GAGCGGACGG TCGCCTGGGT CTCCAACATG CCCCATCTTT CTGCTGACAT AGAGAGTTCC 
CGCATCGACA GAGAAGAGTT CAAGCTGAAG GAGTACTCCA AGAGCATGGA CGAGTCCCGT 
CTGGACCGGG TGCGCGAGTA TGAGGAGGAG ATCCACTCAC TGAAGGAGCG TCTGATGATG 
TCCCACCGCA AGTTGGAAGA GTACGAGAGG AGGCTGTTAA CTCAGGAGCA GCAGACCAAT 
AAAATCCTGC TGCAGTATCA GAGCCGACTG GACGACAGCG AGAGGAGACT CCGACAGCAG 
CAGATGGAGA AAGACAACCA AATTAAAGGA ATTATTGACA GACTCATGGC GGTGGAGGAC 
GAGTTGAGAG TAGGAGTGGT GCCTGAACAC AAACCCAGAG TATTTGCGGA TCAGGGGAGG 
CGGCAGTCCA TCCTAGTGCA GCCCCGCCTT GCGCCCGTCC CACTGCGAAT GCACAGCCAA 
CAACAAAGCT TCATGGAGGA CAATCTGGAA CCTAATTGGA GGCCTCACCT AGGACATTCT 
TCAGGTTGGC CTGGGTTTAA TTCCAGGGCG CTGTCACGCG ATTCGGTTGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290919.1
CDS	316..4749
Translation	MSYVPFQDARYAVPPSYRPQPSFAAAPSYEQADWNPRLGVISGNQLYMLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKSSIRSWFIVSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQGRRQSILVQPRLAPVPLRMHSQQQSFMEDNLEPNWRPHLGHSSGWPGFNSRALSRDSVG

Target ORF information:

RefSeq Version	XM_009292644.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292644.2

ORF Insert Sequence:

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGATTC CAGAATGAAC AGCGTCTCTA ACCTGCAGTC TATGGGGGAC 
ATGCTCAACT CTTCCCAGGC CTCGATTGCT GGCCTCGGCA GCTACGGTGG GCTCGCAGGC 
TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA GCCGGAGGAC GTATGTCGGC CGGATCTGGC 
GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG AGCCAGCTGA GCCAGATGGG AACCACTACC 
GACTCCCTGT CCCAGCAGCA GCAGCAGCAG GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC 
CAGAACCCTC TGTTCCACCT CGCTGCTGAC GGACCGCAGC TTCACCACCA GCACAGTCGA 
GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC CCAGAGCCGG ACGGTTCTCA CCCTACTTAT 
ATCCCACAGT TTGCACATGG TGGTTTCTCC CGAAGTGAGG ACCTCTCCAC CCTCAGACCC 
GGCCCTCATC TGGGCCAGCC AAGTATTATT CATTCTCACA GCTACAGCGA CGACTACAGC 
CGTCATAACC AGAGCGACTA CGGGCGACGA CAGATCTCCA TGCACATGCA GGAGCAGCAG 
CAGATGGCAG GCATGGCATC GCAGACTGGC ACATCTCATT CATCTCTCGC CACTCCTCCT 
TCCACTGTTC AACCTGTTCG TCAAAGCTCC ATGGCTCCTC CCACGCAGCG CATGAAATCA 
CAACCCTCAC ATCAGCTTTC TGTGAGCTCT GCCGCTGGTG CCCCTGCGTC CAAAACCAGG 
CCGCAGAGCG GAAACCTGCT CCAGTCCCCG GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC 
CCTCGGCAGC AGCTGTCTGT CAAAGACAGC ACCCCACCCG GCCTGCCGCA TCAACAGAGC 
TCCACCAGGG AGAGTCAGGG ATCCCAGGGC TCTCAGGGTG GAACGCCGCA GTCCACGCAG 
CAGTCCAAAT CACATCAGGA ACGGCAGCAG GTCCAGCAAC AGCATCTGCT CAAGCCTACC 
ATGAGCAAAC AGGGCTCTTC ACAATCTCCA TCCACCCTGA ACCCGCAGAC CCCTGCCTCT 
GAGCGGACGG TCGCCTGGGT CTCCAACATG CCCCATCTTT CTGCTGACAT AGAGAGTTCC 
CGCATCGACA GAGAAGAGTT CAAGCTGAAG GAGTACTCCA AGAGCATGGA CGAGTCCCGT 
CTGGACCGGG TGCGCGAGTA TGAGGAGGAG ATCCACTCAC TGAAGGAGCG TCTGATGATG 
TCCCACCGCA AGTTGGAAGA GTACGAGAGG AGGCTGTTAA CTCAGGAGCA GCAGACCAAT 
AAAATCCTGC TGCAGTATCA GAGCCGACTG GACGACAGCG AGAGGAGACT CCGACAGCAG 
CAGATGGAGA AAGACAACCA AATTAAAGGA ATTATTGACA GACTCATGGC GGTGGAGGAC 
GAGTTGAGAG TAGGAGTGGT GCCTGAACAC AAACCCAGAG TATTTGCGGA TCAGGGGAGG 
CGGCAGTCCA TCCTAGTGCA GCCCCGCCTT GCGCCCGTCC CACTGCGAAT GCACAGCCAA 
CAACAAAGCT TCATGGAGGA CAATCTGGAA CCTAATTGGA GGCCTCACCT AGGACATTCT 
TCAGGTTGGC CTGGGTTTAA TTCCAGGGCG CTGTCACGCG ATTCGGTTGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_009292645.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290920.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X5, mRNA.

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CloneID	ODa48966
Clone ID Related Accession (Same CDS sequence)	XM_009292645.2
Accession Version	XM_009292645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	si:dkey-246f6.1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGATTC CAGAATGAAC AGCGTCTCTA ACCTGCAGTC TATGGGGGAC 
ATGCTCAACT CTTCCCAGGC CTCGATTGCT GGCCTCGGCA GCTACGGTGG GCTCGCAGGC 
TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA GCCGGAGGAC GTATGTCGGC CGGATCTGGC 
GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG AGCCAGCTGA GCCAGATGGG AACCACTACC 
GACTCCCTGT CCCAGCAGCA GCAGCAGCAG GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC 
CAGAACCCTC TGTTCCACCT CGCTGCTGAC GGACCGCAGC TTCACCACCA GCACAGTCGA 
GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC CCAGAGCCGG ACGGTTCTCA CCCTACTTAT 
ATCCCACAGT TTGCACATGG TGGTTTCTCC CGAAGTGAGG ACCTCTCCAC CCTCAGACCC 
GGCCCTCATC TGGGCCAGCC AAGTATTATT CATTCTCACA GCTACAGCGA CGACTACAGC 
CGTCATAACC AGAGCGACTA CGGGCGACGA CAGATCTCCA TGCACATGCA GGAGCAGCAG 
CAGATGGCAG GCATGGCATC GCAGACTGGC ACATCTCATT CATCTCTCGC CACTCCTCCT 
TCCACTGTTC AACCTGTTCG TCAAAGCTCC ATGGCTCCTC CCACGCAGCG CATGAAATCA 
CAACCCTCAC ATCAGCTTTC TGTGAGCTCT GCCGCTGGTG CCCCTGCGTC CAAAACCAGG 
CCGCAGAGCG GAAACCTGCT CCAGTCCCCG GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC 
CCTCGGCAGC AGCTGTCTGT CAAAGACAGC ACCCCACCCG GCCTGCCGCA TCAACAGAGC 
TCCACCAGGG AGAGTCAGGG ATCCCAGGGC TCTCAGGGTG GAACGCCGCA GTCCACGCAG 
CAGTCCAAAT CACATCAGGA ACGGCAGCAG GTCCAGCAAC AGCATCTGCT CAAGCCTACC 
ATGAGCAAAC AGGGCTCTTC ACAATCTCCA TCCACCCTGA ACCCGCAGAC CCCTGCCTCT 
GAGCGGACGG TCGCCTGGGT CTCCAACATG CCCCATCTTT CTGCTGACAT AGAGAGTTCC 
CGCATCGACA GAGAAGAGTT CAAGCTGAAG GAGTACTCCA AGAGCATGGA CGAGTCCCGT 
CTGGACCGGG TGCGCGAGTA TGAGGAGGAG ATCCACTCAC TGAAGGAGCG TCTGATGATG 
TCCCACCGCA AGTTGGAAGA GTACGAGAGG AGGCTGTTAA CTCAGGAGCA GCAGACCAAT 
AAAATCCTGC TGCAGTATCA GAGCCGACTG GACGACAGCG AGAGGAGACT CCGACAGCAG 
CAGATGGAGA AAGACAACCA AATTAAAGGA ATTATTGACA GACTCATGGC GGTGGAGGAC 
GAGTTGAGAG TAGGAGTGGT GCCTGAACAC AAACCCAGAG TATTTGCGGA TCAGGGGAGG 
CGGCAGTCCA TCCTAGTGCA GCCCCGCCTT GCGCCCGTCC CACTGCGAAT GCACAGGAAA 
AGCAGCTTTC CGCCTTGGGT TCAGCAGACC CCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290920.1
CDS	316..4674
Translation	MSYVPFQDARYAVPPSYRPQPSFAAAPSYEQADWNPRLGVISGNQLYMLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKSSIRSWFIVSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQGRRQSILVQPRLAPVPLRMHRKSSFPPWVQQTPV

Target ORF information:

RefSeq Version	XM_009292645.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292645.2

ORF Insert Sequence:

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGATTC CAGAATGAAC AGCGTCTCTA ACCTGCAGTC TATGGGGGAC 
ATGCTCAACT CTTCCCAGGC CTCGATTGCT GGCCTCGGCA GCTACGGTGG GCTCGCAGGC 
TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA GCCGGAGGAC GTATGTCGGC CGGATCTGGC 
GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG AGCCAGCTGA GCCAGATGGG AACCACTACC 
GACTCCCTGT CCCAGCAGCA GCAGCAGCAG GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC 
CAGAACCCTC TGTTCCACCT CGCTGCTGAC GGACCGCAGC TTCACCACCA GCACAGTCGA 
GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC CCAGAGCCGG ACGGTTCTCA CCCTACTTAT 
ATCCCACAGT TTGCACATGG TGGTTTCTCC CGAAGTGAGG ACCTCTCCAC CCTCAGACCC 
GGCCCTCATC TGGGCCAGCC AAGTATTATT CATTCTCACA GCTACAGCGA CGACTACAGC 
CGTCATAACC AGAGCGACTA CGGGCGACGA CAGATCTCCA TGCACATGCA GGAGCAGCAG 
CAGATGGCAG GCATGGCATC GCAGACTGGC ACATCTCATT CATCTCTCGC CACTCCTCCT 
TCCACTGTTC AACCTGTTCG TCAAAGCTCC ATGGCTCCTC CCACGCAGCG CATGAAATCA 
CAACCCTCAC ATCAGCTTTC TGTGAGCTCT GCCGCTGGTG CCCCTGCGTC CAAAACCAGG 
CCGCAGAGCG GAAACCTGCT CCAGTCCCCG GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC 
CCTCGGCAGC AGCTGTCTGT CAAAGACAGC ACCCCACCCG GCCTGCCGCA TCAACAGAGC 
TCCACCAGGG AGAGTCAGGG ATCCCAGGGC TCTCAGGGTG GAACGCCGCA GTCCACGCAG 
CAGTCCAAAT CACATCAGGA ACGGCAGCAG GTCCAGCAAC AGCATCTGCT CAAGCCTACC 
ATGAGCAAAC AGGGCTCTTC ACAATCTCCA TCCACCCTGA ACCCGCAGAC CCCTGCCTCT 
GAGCGGACGG TCGCCTGGGT CTCCAACATG CCCCATCTTT CTGCTGACAT AGAGAGTTCC 
CGCATCGACA GAGAAGAGTT CAAGCTGAAG GAGTACTCCA AGAGCATGGA CGAGTCCCGT 
CTGGACCGGG TGCGCGAGTA TGAGGAGGAG ATCCACTCAC TGAAGGAGCG TCTGATGATG 
TCCCACCGCA AGTTGGAAGA GTACGAGAGG AGGCTGTTAA CTCAGGAGCA GCAGACCAAT 
AAAATCCTGC TGCAGTATCA GAGCCGACTG GACGACAGCG AGAGGAGACT CCGACAGCAG 
CAGATGGAGA AAGACAACCA AATTAAAGGA ATTATTGACA GACTCATGGC GGTGGAGGAC 
GAGTTGAGAG TAGGAGTGGT GCCTGAACAC AAACCCAGAG TATTTGCGGA TCAGGGGAGG 
CGGCAGTCCA TCCTAGTGCA GCCCCGCCTT GCGCCCGTCC CACTGCGAAT GCACAGGAAA 
AGCAGCTTTC CGCCTTGGGT TCAGCAGACC CCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_009292646.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290921.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa48967
Clone ID Related Accession (Same CDS sequence)	XM_009292646.2
Accession Version	XM_009292646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	si:dkey-246f6.1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGATTC CAGAATGAAC AGCGTCTCTA ACCTGCAGTC TATGGGGGAC 
ATGCTCAACT CTTCCCAGGC CTCGATTGCT GGCCTCGGCA GCTACGGTGG GCTCGCAGGC 
TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA GCCGGAGGAC GTATGTCGGC CGGATCTGGC 
GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG AGCCAGCTGA GCCAGATGGG AACCACTACC 
GACTCCCTGT CCCAGCAGCA GCAGCAGCAG GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC 
CAGAACCCTC TGTTCCACCT CGCTGCTGAC GGACCGCAGC TTCACCACCA GCACAGTCGA 
GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC CCAGAGCCGG ACGGTTCTCA CCCTACTTAT 
ATCCCACAGT TTGCACATGG TGGTTTCTCC CGAAGTGAGG ACCTCTCCAC CCTCAGACCC 
GGCCCTCATC TGGGCCAGCC AAGTATTATT CATTCTCACA GCTACAGCGA CGACTACAGC 
CGTCATAACC AGAGCGACTA CGGGCGACGA CAGATCTCCA TGCACATGCA GGAGCAGCAG 
CAGATGGCAG GCATGGCATC GCAGACTGGC ACATCTCATT CATCTCTCGC CACTCCTCCT 
TCCACTGTTC AACCTGTTCG TCAAAGCTCC ATGGCTCCTC CCACGCAGCG CATGAAATCA 
CAACCCTCAC ATCAGCTTTC TGTGAGCTCT GCCGCTGGTG CCCCTGCGTC CAAAACCAGG 
CCGCAGAGCG GAAACCTGCT CCAGTCCCCG GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC 
CCTCGGCAGC AGCTGTCTGT CAAAGACAGC ACCCCACCCG GCCTGCCGCA TCAACAGAGC 
TCCACCAGGG AGAGTCAGGG ATCCCAGGGC TCTCAGGGTG GAACGCCGCA GTCCACGCAG 
CAGTCCAAAT CACATCAGGA ACGGCAGCAG GTCCAGCAAC AGCATCTGCT CAAGCCTACC 
ATGAGCAAAC AGGGCTCTTC ACAATCTCCA TCCACCCTGA ACCCGCAGAC CCCTGCCTCT 
GAGCGGACGG TCGCCTGGGT CTCCAACATG CCCCATCTTT CTGCTGACAT AGAGAGTTCC 
CGCATCGACA GAGAAGAGTT CAAGCTGAAG GAGTACTCCA AGAGCATGGA CGAGTCCCGT 
CTGGACCGGG TGCGCGAGTA TGAGGAGGAG ATCCACTCAC TGAAGGAGCG TCTGATGATG 
TCCCACCGCA AGTTGGAAGA GTACGAGAGG AGGCTGTTAA CTCAGGAGCA GCAGACCAAT 
AAAATCCTGC TGCAGTATCA GAGCCGACTG GACGACAGCG AGAGGAGACT CCGACAGCAG 
CAGATGGAGA AAGACAACCA AATTAAAGGA ATTATTGACA GACTCATGGC GGTGGAGGAC 
GAGTTGAGAG TAGGAGTGGT GCCTGAACAC AAACCCAGAG TATTTGCGGA TCAGACTTCA 
CTGAAAAGGA CTGATCATGA TGAAACACAC ACTGAACATG ATTTAGATAT AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290921.1
CDS	316..4632
Translation	MSYVPFQDARYAVPPSYRPQPSFAAAPSYEQADWNPRLGVISGNQLYMLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKSSIRSWFIVSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQTSLKRTDHDETHTEHDLDIK

Target ORF information:

RefSeq Version	XM_009292646.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292646.2

ORF Insert Sequence:

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGATTC CAGAATGAAC AGCGTCTCTA ACCTGCAGTC TATGGGGGAC 
ATGCTCAACT CTTCCCAGGC CTCGATTGCT GGCCTCGGCA GCTACGGTGG GCTCGCAGGC 
TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA GCCGGAGGAC GTATGTCGGC CGGATCTGGC 
GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG AGCCAGCTGA GCCAGATGGG AACCACTACC 
GACTCCCTGT CCCAGCAGCA GCAGCAGCAG GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC 
CAGAACCCTC TGTTCCACCT CGCTGCTGAC GGACCGCAGC TTCACCACCA GCACAGTCGA 
GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC CCAGAGCCGG ACGGTTCTCA CCCTACTTAT 
ATCCCACAGT TTGCACATGG TGGTTTCTCC CGAAGTGAGG ACCTCTCCAC CCTCAGACCC 
GGCCCTCATC TGGGCCAGCC AAGTATTATT CATTCTCACA GCTACAGCGA CGACTACAGC 
CGTCATAACC AGAGCGACTA CGGGCGACGA CAGATCTCCA TGCACATGCA GGAGCAGCAG 
CAGATGGCAG GCATGGCATC GCAGACTGGC ACATCTCATT CATCTCTCGC CACTCCTCCT 
TCCACTGTTC AACCTGTTCG TCAAAGCTCC ATGGCTCCTC CCACGCAGCG CATGAAATCA 
CAACCCTCAC ATCAGCTTTC TGTGAGCTCT GCCGCTGGTG CCCCTGCGTC CAAAACCAGG 
CCGCAGAGCG GAAACCTGCT CCAGTCCCCG GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC 
CCTCGGCAGC AGCTGTCTGT CAAAGACAGC ACCCCACCCG GCCTGCCGCA TCAACAGAGC 
TCCACCAGGG AGAGTCAGGG ATCCCAGGGC TCTCAGGGTG GAACGCCGCA GTCCACGCAG 
CAGTCCAAAT CACATCAGGA ACGGCAGCAG GTCCAGCAAC AGCATCTGCT CAAGCCTACC 
ATGAGCAAAC AGGGCTCTTC ACAATCTCCA TCCACCCTGA ACCCGCAGAC CCCTGCCTCT 
GAGCGGACGG TCGCCTGGGT CTCCAACATG CCCCATCTTT CTGCTGACAT AGAGAGTTCC 
CGCATCGACA GAGAAGAGTT CAAGCTGAAG GAGTACTCCA AGAGCATGGA CGAGTCCCGT 
CTGGACCGGG TGCGCGAGTA TGAGGAGGAG ATCCACTCAC TGAAGGAGCG TCTGATGATG 
TCCCACCGCA AGTTGGAAGA GTACGAGAGG AGGCTGTTAA CTCAGGAGCA GCAGACCAAT 
AAAATCCTGC TGCAGTATCA GAGCCGACTG GACGACAGCG AGAGGAGACT CCGACAGCAG 
CAGATGGAGA AAGACAACCA AATTAAAGGA ATTATTGACA GACTCATGGC GGTGGAGGAC 
GAGTTGAGAG TAGGAGTGGT GCCTGAACAC AAACCCAGAG TATTTGCGGA TCAGACTTCA 
CTGAAAAGGA CTGATCATGA TGAAACACAC ACTGAACATG ATTTAGATAT AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_009292648.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290923.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X7, mRNA.

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CloneID	ODa48968
Clone ID Related Accession (Same CDS sequence)	XM_009292648.2
Accession Version	XM_009292648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	si:dkey-246f6.1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGATTC CAGAATGAAC AGCGTCTCTA ACCTGCAGTC TATGGGGGAC 
ATGCTCAACT CTTCCCAGGC CTCGATTGCT GGCCTCGGCA GCTACGGTGG GCTCGCAGGC 
TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA GCCGGAGGAC GTATGTCGGC CGGATCTGGC 
GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG AGCCAGCTGA GCCAGATGGG AACCACTACC 
GACTCCCTGT CCCAGCAGCA GCAGCAGCAG GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC 
CAGAACCCTC TGTTCCACCT CGCTGCTGAC GGACCGCAGC TTCACCACCA GCACAGTCGA 
GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC CCAGAGCCGG ACGGTTCTCA CCCTACTTAT 
ATCCCACAGT TTGCACATGG TGGTTTCTCC CGAAGTGAGG ACCTCTCCAC CCTCAGACCC 
GGCCCTCATC TGGGCCAGCC AAGTATTATT CATTCTCACA GCTACAGCGA CGACTACAGC 
CGTCATAACC AGAGCGACTA CGGGCGACGA CAGATCTCCA TGCACATGCA GGAGCAGCAG 
CAGATGGCAG GCATGGCATC GCAGACTGGC ACATCTCATT CATCTCTCGC CACTCCTCCT 
TCCACTGTTC AACCTGTTCG TCAAAGCTCC ATGGCTCCTC CCACGCAGCG CATGAAATCA 
CAACCCTCAC ATCAGCTTTC TGTGAGCTCT GCCGCTGGTG CCCCTGCGTC CAAAACCAGG 
CCGCAGAGCG GAAACCTGCT CCAGTCCCCG GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC 
CCTCGGCAGC AGCTGTCTGT CAAAGACAGC ACCCCACCCG GCCTGCCGCA TCAACAGAGC 
TCCACCAGGG AGAGTCAGGG ATCCCAGGGC TCTCAGGGTG GAACGCCGCA GTCCACGCAG 
CAGTCCAAAT CACATCAGGA ACGGCAGCAG GTCCAGCAAC AGCATCTGCT CAAGCCTACC 
ATGAGCAAAC AGGGCTCTTC ACAATCTCCA TCCACCCTGA ACCCGCAGAC CCCTGCCTCT 
GAGCGGACGG TCGCCTGGGT CTCCAACATG CCCCATCTTT CTGCTGACAT AGAGAGTTCC 
CGCATCGACA GAGAAGAGTT CAAGCTGAAG GAGTACTCCA AGAGCATGGA CGAGTCCCGT 
CTGGACCGGG TGCGCGAGTA TGAGGAGGAG ATCCACTCAC TGAAGGAGCG TCTGATGATG 
TCCCACCGCA AGTTGGAAGA GTACGAGAGG AGGCTGTTAA CTCAGGAGCA GCAGACCAAT 
AAAATCCTGC TGCAGTATCA GAGCCGACTG GACGACAGCG AGAGGAGACT CCGACAGCAG 
CAGATGGAGA AAGACAACCA AATTAAAGGA ATTATTGACA GACTCATGGC GGTGGAGGAC 
GAGTTGAGAG TAGGAGTGGT GCCTGAACAC AAACCCAGAG TATTTGCGGA TCAGCCAACA 
ACAAAGCTTC ATGGAGGACA ATCTGGAACC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290923.1
CDS	316..4608
Translation	MSYVPFQDARYAVPPSYRPQPSFAAAPSYEQADWNPRLGVISGNQLYMLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKSSIRSWFIVSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQPTTKLHGGQSGT

Target ORF information:

RefSeq Version	XM_009292648.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292648.2

ORF Insert Sequence:

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGATTC CAGAATGAAC AGCGTCTCTA ACCTGCAGTC TATGGGGGAC 
ATGCTCAACT CTTCCCAGGC CTCGATTGCT GGCCTCGGCA GCTACGGTGG GCTCGCAGGC 
TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA GCCGGAGGAC GTATGTCGGC CGGATCTGGC 
GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG AGCCAGCTGA GCCAGATGGG AACCACTACC 
GACTCCCTGT CCCAGCAGCA GCAGCAGCAG GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC 
CAGAACCCTC TGTTCCACCT CGCTGCTGAC GGACCGCAGC TTCACCACCA GCACAGTCGA 
GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC CCAGAGCCGG ACGGTTCTCA CCCTACTTAT 
ATCCCACAGT TTGCACATGG TGGTTTCTCC CGAAGTGAGG ACCTCTCCAC CCTCAGACCC 
GGCCCTCATC TGGGCCAGCC AAGTATTATT CATTCTCACA GCTACAGCGA CGACTACAGC 
CGTCATAACC AGAGCGACTA CGGGCGACGA CAGATCTCCA TGCACATGCA GGAGCAGCAG 
CAGATGGCAG GCATGGCATC GCAGACTGGC ACATCTCATT CATCTCTCGC CACTCCTCCT 
TCCACTGTTC AACCTGTTCG TCAAAGCTCC ATGGCTCCTC CCACGCAGCG CATGAAATCA 
CAACCCTCAC ATCAGCTTTC TGTGAGCTCT GCCGCTGGTG CCCCTGCGTC CAAAACCAGG 
CCGCAGAGCG GAAACCTGCT CCAGTCCCCG GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC 
CCTCGGCAGC AGCTGTCTGT CAAAGACAGC ACCCCACCCG GCCTGCCGCA TCAACAGAGC 
TCCACCAGGG AGAGTCAGGG ATCCCAGGGC TCTCAGGGTG GAACGCCGCA GTCCACGCAG 
CAGTCCAAAT CACATCAGGA ACGGCAGCAG GTCCAGCAAC AGCATCTGCT CAAGCCTACC 
ATGAGCAAAC AGGGCTCTTC ACAATCTCCA TCCACCCTGA ACCCGCAGAC CCCTGCCTCT 
GAGCGGACGG TCGCCTGGGT CTCCAACATG CCCCATCTTT CTGCTGACAT AGAGAGTTCC 
CGCATCGACA GAGAAGAGTT CAAGCTGAAG GAGTACTCCA AGAGCATGGA CGAGTCCCGT 
CTGGACCGGG TGCGCGAGTA TGAGGAGGAG ATCCACTCAC TGAAGGAGCG TCTGATGATG 
TCCCACCGCA AGTTGGAAGA GTACGAGAGG AGGCTGTTAA CTCAGGAGCA GCAGACCAAT 
AAAATCCTGC TGCAGTATCA GAGCCGACTG GACGACAGCG AGAGGAGACT CCGACAGCAG 
CAGATGGAGA AAGACAACCA AATTAAAGGA ATTATTGACA GACTCATGGC GGTGGAGGAC 
GAGTTGAGAG TAGGAGTGGT GCCTGAACAC AAACCCAGAG TATTTGCGGA TCAGCCAACA 
ACAAAGCTTC ATGGAGGACA ATCTGGAACC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_009292649.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290924.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa48969
Clone ID Related Accession (Same CDS sequence)	XM_009292649.2
Accession Version	XM_009292649.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	si:dkey-246f6.1 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
AGTTTCCTCA GTAGAAGACT GAAGGGGTCC ATTAAACGGG CCAAGAGTCA GCCCAAGCTG 
GACCGAACCA GCAGCTTCCG CCACATGATC CTCCCTCGCT TCCGCAGCGC CGACCAGGAG 
CGGACTCGTC TGATGCAGAG CTTCAAAGAA TCACACTCCC ATGAGTCTTT GCTGTCTCCC 
AGCAGCGCAG CCGAGGCCTT GGATTTGACA CTTGATGAAG ACGCCATCAT CAAGCCTGTT 
CACTCCAGTA TATTGGGACA GGAGTACTGT TTCGAGGTGA TAACTGCTTC AGGAACAAAG 
TGTTTTGCCT GTCGGTCGGC TGCGGAGCGA GACAAATGGA TCGAGAATCT ACAGAGAGCT 
GTCAAACCCA ACAAAGACAA CAGTCGGCGT GTCGACAACG TCCTGAAGCT GTGGATCATT 
GAGGCCCGTG AGCTCCCACC CAAAAAGCGC TATTATTGTG AACTGTGCTT GGATGACATG 
CTGTACGCTC GCACCACCAG CAAACCCCGT ACCGACACTG TCTTCTGGGG TGAGCACTTT 
GAGTTCAACA ACCTGCCTGC TGTCCGCAAC CTCCGCCTTC ATCTGTACAA AGAGACGGAC 
AAGAAGAGAC GCAAGGAGAA GAGCACGTAC CTTGGCTTGG TCAGCATTCC CATTTCCAGC 
ATCACTGGCC GTCAGTTTGT GGAGCAATGG TACCCCGTCA TTCAGCCCAG TGTTCTGGCC 
AAGGGTGGCG GCGTCGGAGG AGGGAAGATC ATCAATGCCT CGCTGCGGCT CAAGTCACGC 
TTTCAGACCA TGAGCATCCT GCCGATGGAA CTGTATAAAG AGTTCGCAGA GTACGTCACA 
AACAACTACC GGACGCTGTG TGCGGTCCTG GAGCCACTGC TGAGTGTCAA GAGCAAAGAA 
GAAGTGGCCT GTGCTCTGGT GCACATCCTA CAGAGCACAG GGAAGGCCAA GGATTTTCTG 
TCAGACATGG CGATGTGTGA GGTGGATCGC TTCATCGACC GCGAGCACCT GATCTTCAGA 
GAAAACACCC TGGCCACCAA AGCCATAGAA GAGTACCTCA AACTCATCGG ACACAAGTAC 
CTCAAAGATG TTTTAGGTGA TTTTATCCGA GCGCTGTATG AATCAGAGGA GAACTGTGAA 
GTGGACCCGA TGCGAACTCC CCCTTCAATA CTGCCCGAAC ATCAAGCCAA CCTCCGGATG 
TGCTGTGAGC TCGCCCTCTG CAAAATCATC AACTCATATT GTGCGTTCCC GCGAGAGCTG 
AAGGAAGTGT TTGCATCATG GCGTGTCCGC TGTGCTGAGA GAGGAAGAGA AGACATTGCA 
GATCGACTGA TCAGCGGCTC CCTCTTCCTG CGCTTCCTCT GTCCAGCCAT CATGTCTCCA 
TCTCTGTTCA ACCTCACGCA GGAATATCCC GATGAGCAGA CCTCACGAAC ACTCACACTC 
ATCGCCAAAG TGGTCCAGAA CCTCGCAAAC TTCTCCAAGT TTGGCAGTAA GGAGGAGTAC 
ATGTGCTTCA TGAATGAGTT TCTGGAGATG GAGTGGGGCT CCATGCAGCA GTTTCTCTAT 
GAGATCTCCA ATCTGGACAG TGTGAGCAAT GCTGGGGCCT TCGAGGGCTA CATTGACCTA 
GGCCGCGAGC TCTCCATCCT GCACAGTCTG CTCTGGGAGG TTATGGCTCA GCTGAGCAAG 
TCATCAATTA GGTCTTGGTT TATTGTGTCA AAGGATGCCA TCATCAAACT TGGACCATTG 
CCCCGCCTCC TCAATGACAT CAGCATGGCC CTCCGCAACC CTCACCTGCA GAGGCAGCCC 
AGTCATCAGA CAGACAGACA GCCTGCAGAG AGACAGACAG ACCGACTGCT GTCCCGACCC 
AGCTTCAACA GGGGCGTCTC GTCTGAGTTC CAGAATCTCA TGATGAGAGA CCTTAACAGT 
TCAGTGGAGA TCACTCGTCT GCCCTCTCCA ACTTCTGCCA TGTCCAGCGG TGGAGCTCCT 
CCATCCCGTG CAGGAATGGC TGGGTTTGCG GACCGCGATC ATCCTCACCG AGCCTCGTCT 
AAAGATGTTT TCTATGGTGC CCGTCCCCCA CTGGCCCGCT CCAGTCCAGC GTACTGTACT 
AGCAGTTCAG ATATTACTGA TCCAGACCCA AAGGATTCCA GAATGAACAG CGTCTCTAAC 
CTGCAGTCTA TGGGGGACAT GCTCAACTCT TCCCAGGCCT CGATTGCTGG CCTCGGCAGC 
TACGGTGGGC TCGCAGGCTT GGGTGGAGGC CTCGGAGGTC AGCTGCGAGC CGGAGGACGT 
ATGTCGGCCG GATCTGGCGG TTCCAGTATG TCCGGGGGTC TGAGGTTGAG CCAGCTGAGC 
CAGATGGGAA CCACTACCGA CTCCCTGTCC CAGCAGCAGC AGCAGCAGGC CGCCGCCCTC 
CGCTTTCCCC TGTCCTTCCA GAACCCTCTG TTCCACCTCG CTGCTGACGG ACCGCAGCTT 
CACCACCAGC ACAGTCGAGC TCAGCCTCCT CCTCCGCTCC TGCTGGCCCC AGAGCCGGAC 
GGTTCTCACC CTACTTATAT CCCACAGTTT GCACATGGTG GTTTCTCCCG AAGTGAGGAC 
CTCTCCACCC TCAGACCCGG CCCTCATCTG GGCCAGCCAA GTATTATTCA TTCTCACAGC 
TACAGCGACG ACTACAGCCG TCATAACCAG AGCGACTACG GGCGACGACA GATCTCCATG 
CACATGCAGG AGCAGCAGCA GATGGCAGGC ATGGCATCGC AGACTGGCAC ATCTCATTCA 
TCTCTCGCCA CTCCTCCTTC CACTGTTCAA CCTGTTCGTC AAAGCTCCAT GGCTCCTCCC 
ACGCAGCGCA TGAAATCACA ACCCTCACAT CAGCTTTCTG TGAGCTCTGC CGCTGGTGCC 
CCTGCGTCCA AAACCAGGCC GCAGAGCGGA AACCTGCTCC AGTCCCCGGA GTCTGGCTTC 
GGGGGCCGGC AGCAGGGCCC TCGGCAGCAG CTGTCTGTCA AAGACAGCAC CCCACCCGGC 
CTGCCGCATC AACAGAGCTC CACCAGGGAG AGTCAGGGAT CCCAGGGCTC TCAGGGTGGA 
ACGCCGCAGT CCACGCAGCA GTCCAAATCA CATCAGGAAC GGCAGCAGGT CCAGCAACAG 
CATCTGCTCA AGCCTACCAT GAGCAAACAG GGCTCTTCAC AATCTCCATC CACCCTGAAC 
CCGCAGACCC CTGCCTCTGA GCGGACGGTC GCCTGGGTCT CCAACATGCC CCATCTTTCT 
GCTGACATAG AGAGTTCCCG CATCGACAGA GAAGAGTTCA AGCTGAAGGA GTACTCCAAG 
AGCATGGACG AGTCCCGTCT GGACCGGGTG CGCGAGTATG AGGAGGAGAT CCACTCACTG 
AAGGAGCGTC TGATGATGTC CCACCGCAAG TTGGAAGAGT ACGAGAGGAG GCTGTTAACT 
CAGGAGCAGC AGACCAATAA AATCCTGCTG CAGTATCAGA GCCGACTGGA CGACAGCGAG 
AGGAGACTCC GACAGCAGCA GATGGAGAAA GACAACCAAA TTAAAGGAAT TATTGACAGA 
CTCATGGCGG TGGAGGACGA GTTGAGAGTA GGAGTGGTGC CTGAACACAA ACCCAGAGTA 
TTTGCGGATC AGGAAAAGCA GCTTTCCGCC TTGGGTTCAG CAGACCCCGG TGTGAGTTTT 
GGAGGAGAAA TGACCAGCGC TTCTGGCTTT GTTGGAGTCT CCCCTTCGGA CAAATCCTCC 
ACCCGATCCC CCCAGAATGG AATCGCGTCC TTCCCGTTAG TTCCCCCTCC TCGTTCAATT 
CCTCACGCAC AACTCTGGCC GAACGGCAAC CAAAAATCTG CAATGGATTG GAGGGCAAGT 
TTGGAGGGGG AGCAGGTCAA TAACAACGCT AATCAAATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290924.1
CDS	316..4557
Translation	MSYVPFQDARYAVPPSYRPQPSFAAAPSYEQADWNPRLGVISGNQLYMLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSSFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKSSIRSWFIVSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQEKQLSALGSADPGVSFGGEMTSASGFVGVSPSDKSSTRSPQNGIASFPLVPPPRSIPHAQLWPNGNQKSAMDWRASLEGEQVNNNANQI

Target ORF information:

RefSeq Version	XM_009292649.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292649.2

ORF Insert Sequence:

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
AGTTTCCTCA GTAGAAGACT GAAGGGGTCC ATTAAACGGG CCAAGAGTCA GCCCAAGCTG 
GACCGAACCA GCAGCTTCCG CCACATGATC CTCCCTCGCT TCCGCAGCGC CGACCAGGAG 
CGGACTCGTC TGATGCAGAG CTTCAAAGAA TCACACTCCC ATGAGTCTTT GCTGTCTCCC 
AGCAGCGCAG CCGAGGCCTT GGATTTGACA CTTGATGAAG ACGCCATCAT CAAGCCTGTT 
CACTCCAGTA TATTGGGACA GGAGTACTGT TTCGAGGTGA TAACTGCTTC AGGAACAAAG 
TGTTTTGCCT GTCGGTCGGC TGCGGAGCGA GACAAATGGA TCGAGAATCT ACAGAGAGCT 
GTCAAACCCA ACAAAGACAA CAGTCGGCGT GTCGACAACG TCCTGAAGCT GTGGATCATT 
GAGGCCCGTG AGCTCCCACC CAAAAAGCGC TATTATTGTG AACTGTGCTT GGATGACATG 
CTGTACGCTC GCACCACCAG CAAACCCCGT ACCGACACTG TCTTCTGGGG TGAGCACTTT 
GAGTTCAACA ACCTGCCTGC TGTCCGCAAC CTCCGCCTTC ATCTGTACAA AGAGACGGAC 
AAGAAGAGAC GCAAGGAGAA GAGCACGTAC CTTGGCTTGG TCAGCATTCC CATTTCCAGC 
ATCACTGGCC GTCAGTTTGT GGAGCAATGG TACCCCGTCA TTCAGCCCAG TGTTCTGGCC 
AAGGGTGGCG GCGTCGGAGG AGGGAAGATC ATCAATGCCT CGCTGCGGCT CAAGTCACGC 
TTTCAGACCA TGAGCATCCT GCCGATGGAA CTGTATAAAG AGTTCGCAGA GTACGTCACA 
AACAACTACC GGACGCTGTG TGCGGTCCTG GAGCCACTGC TGAGTGTCAA GAGCAAAGAA 
GAAGTGGCCT GTGCTCTGGT GCACATCCTA CAGAGCACAG GGAAGGCCAA GGATTTTCTG 
TCAGACATGG CGATGTGTGA GGTGGATCGC TTCATCGACC GCGAGCACCT GATCTTCAGA 
GAAAACACCC TGGCCACCAA AGCCATAGAA GAGTACCTCA AACTCATCGG ACACAAGTAC 
CTCAAAGATG TTTTAGGTGA TTTTATCCGA GCGCTGTATG AATCAGAGGA GAACTGTGAA 
GTGGACCCGA TGCGAACTCC CCCTTCAATA CTGCCCGAAC ATCAAGCCAA CCTCCGGATG 
TGCTGTGAGC TCGCCCTCTG CAAAATCATC AACTCATATT GTGCGTTCCC GCGAGAGCTG 
AAGGAAGTGT TTGCATCATG GCGTGTCCGC TGTGCTGAGA GAGGAAGAGA AGACATTGCA 
GATCGACTGA TCAGCGGCTC CCTCTTCCTG CGCTTCCTCT GTCCAGCCAT CATGTCTCCA 
TCTCTGTTCA ACCTCACGCA GGAATATCCC GATGAGCAGA CCTCACGAAC ACTCACACTC 
ATCGCCAAAG TGGTCCAGAA CCTCGCAAAC TTCTCCAAGT TTGGCAGTAA GGAGGAGTAC 
ATGTGCTTCA TGAATGAGTT TCTGGAGATG GAGTGGGGCT CCATGCAGCA GTTTCTCTAT 
GAGATCTCCA ATCTGGACAG TGTGAGCAAT GCTGGGGCCT TCGAGGGCTA CATTGACCTA 
GGCCGCGAGC TCTCCATCCT GCACAGTCTG CTCTGGGAGG TTATGGCTCA GCTGAGCAAG 
TCATCAATTA GGTCTTGGTT TATTGTGTCA AAGGATGCCA TCATCAAACT TGGACCATTG 
CCCCGCCTCC TCAATGACAT CAGCATGGCC CTCCGCAACC CTCACCTGCA GAGGCAGCCC 
AGTCATCAGA CAGACAGACA GCCTGCAGAG AGACAGACAG ACCGACTGCT GTCCCGACCC 
AGCTTCAACA GGGGCGTCTC GTCTGAGTTC CAGAATCTCA TGATGAGAGA CCTTAACAGT 
TCAGTGGAGA TCACTCGTCT GCCCTCTCCA ACTTCTGCCA TGTCCAGCGG TGGAGCTCCT 
CCATCCCGTG CAGGAATGGC TGGGTTTGCG GACCGCGATC ATCCTCACCG AGCCTCGTCT 
AAAGATGTTT TCTATGGTGC CCGTCCCCCA CTGGCCCGCT CCAGTCCAGC GTACTGTACT 
AGCAGTTCAG ATATTACTGA TCCAGACCCA AAGGATTCCA GAATGAACAG CGTCTCTAAC 
CTGCAGTCTA TGGGGGACAT GCTCAACTCT TCCCAGGCCT CGATTGCTGG CCTCGGCAGC 
TACGGTGGGC TCGCAGGCTT GGGTGGAGGC CTCGGAGGTC AGCTGCGAGC CGGAGGACGT 
ATGTCGGCCG GATCTGGCGG TTCCAGTATG TCCGGGGGTC TGAGGTTGAG CCAGCTGAGC 
CAGATGGGAA CCACTACCGA CTCCCTGTCC CAGCAGCAGC AGCAGCAGGC CGCCGCCCTC 
CGCTTTCCCC TGTCCTTCCA GAACCCTCTG TTCCACCTCG CTGCTGACGG ACCGCAGCTT 
CACCACCAGC ACAGTCGAGC TCAGCCTCCT CCTCCGCTCC TGCTGGCCCC AGAGCCGGAC 
GGTTCTCACC CTACTTATAT CCCACAGTTT GCACATGGTG GTTTCTCCCG AAGTGAGGAC 
CTCTCCACCC TCAGACCCGG CCCTCATCTG GGCCAGCCAA GTATTATTCA TTCTCACAGC 
TACAGCGACG ACTACAGCCG TCATAACCAG AGCGACTACG GGCGACGACA GATCTCCATG 
CACATGCAGG AGCAGCAGCA GATGGCAGGC ATGGCATCGC AGACTGGCAC ATCTCATTCA 
TCTCTCGCCA CTCCTCCTTC CACTGTTCAA CCTGTTCGTC AAAGCTCCAT GGCTCCTCCC 
ACGCAGCGCA TGAAATCACA ACCCTCACAT CAGCTTTCTG TGAGCTCTGC CGCTGGTGCC 
CCTGCGTCCA AAACCAGGCC GCAGAGCGGA AACCTGCTCC AGTCCCCGGA GTCTGGCTTC 
GGGGGCCGGC AGCAGGGCCC TCGGCAGCAG CTGTCTGTCA AAGACAGCAC CCCACCCGGC 
CTGCCGCATC AACAGAGCTC CACCAGGGAG AGTCAGGGAT CCCAGGGCTC TCAGGGTGGA 
ACGCCGCAGT CCACGCAGCA GTCCAAATCA CATCAGGAAC GGCAGCAGGT CCAGCAACAG 
CATCTGCTCA AGCCTACCAT GAGCAAACAG GGCTCTTCAC AATCTCCATC CACCCTGAAC 
CCGCAGACCC CTGCCTCTGA GCGGACGGTC GCCTGGGTCT CCAACATGCC CCATCTTTCT 
GCTGACATAG AGAGTTCCCG CATCGACAGA GAAGAGTTCA AGCTGAAGGA GTACTCCAAG 
AGCATGGACG AGTCCCGTCT GGACCGGGTG CGCGAGTATG AGGAGGAGAT CCACTCACTG 
AAGGAGCGTC TGATGATGTC CCACCGCAAG TTGGAAGAGT ACGAGAGGAG GCTGTTAACT 
CAGGAGCAGC AGACCAATAA AATCCTGCTG CAGTATCAGA GCCGACTGGA CGACAGCGAG 
AGGAGACTCC GACAGCAGCA GATGGAGAAA GACAACCAAA TTAAAGGAAT TATTGACAGA 
CTCATGGCGG TGGAGGACGA GTTGAGAGTA GGAGTGGTGC CTGAACACAA ACCCAGAGTA 
TTTGCGGATC AGGAAAAGCA GCTTTCCGCC TTGGGTTCAG CAGACCCCGG TGTGAGTTTT 
GGAGGAGAAA TGACCAGCGC TTCTGGCTTT GTTGGAGTCT CCCCTTCGGA CAAATCCTCC 
ACCCGATCCC CCCAGAATGG AATCGCGTCC TTCCCGTTAG TTCCCCCTCC TCGTTCAATT 
CCTCACGCAC AACTCTGGCC GAACGGCAAC CAAAAATCTG CAATGGATTG GAGGGCAAGT 
TTGGAGGGGG AGCAGGTCAA TAACAACGCT AATCAAATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_009292650.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290925.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa48970
Clone ID Related Accession (Same CDS sequence)	XM_009292650.2
Accession Version	XM_009292650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	si:dkey-246f6.1 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATCC CACCCACTGC CCCCCCACAA CCCTTCCGAC AATCCGTGAG TAACCCACCT 
TTCCGTCCCC GGTCCCTCTC TCCATCCCTG TCCCTATCGC TGCCGTTCGC TCCATCGCCC 
CTGTCGCCCT CGCTGTCCCC GTCCCATTTA CAGTCCCAAA AGCGAATCAG CACTCCTCCG 
CTGCCTCGTT CTCTCCCTCT CTCCCCGAGC GTCTCTCTGA CTCTCCCACC CTCTGTCCCG 
CTCTCCCACT GGGCTGCTGG TGAGTGGAAG GTTGTGGGTT CTAATCCAGG GACGGGATCC 
AGTGTGGCGG CACTGCCCTC TCTTGAGAGT TTCCTCAGTA GAAGACTGAA GGGGTCCATT 
AAACGGGCCA AGAGTCAGCC CAAGCTGGAC CGAACCAGCA GCTTCCGCCA CATGATCCTC 
CCTCGCTTCC GCAGCGCCGA CCAGGAGCGG ACTCGTCTGA TGCAGAGCTT CAAAGAATCA 
CACTCCCATG AGTCTTTGCT GTCTCCCAGC AGCGCAGCCG AGGCCTTGGA TTTGACACTT 
GATGAAGACG CCATCATCAA GCCTGTTCAC TCCAGTATAT TGGGACAGGA GTACTGTTTC 
GAGGTGATAA CTGCTTCAGG AACAAAGTGT TTTGCCTGTC GGTCGGCTGC GGAGCGAGAC 
AAATGGATCG AGAATCTACA GAGAGCTGTC AAACCCAACA AAGACAACAG TCGGCGTGTC 
GACAACGTCC TGAAGCTGTG GATCATTGAG GCCCGTGAGC TCCCACCCAA AAAGCGCTAT 
TATTGTGAAC TGTGCTTGGA TGACATGCTG TACGCTCGCA CCACCAGCAA ACCCCGTACC 
GACACTGTCT TCTGGGGTGA GCACTTTGAG TTCAACAACC TGCCTGCTGT CCGCAACCTC 
CGCCTTCATC TGTACAAAGA GACGGACAAG AAGAGACGCA AGGAGAAGAG CACGTACCTT 
GGCTTGGTCA GCATTCCCAT TTCCAGCATC ACTGGCCGTC AGTTTGTGGA GCAATGGTAC 
CCCGTCATTC AGCCCAGTGT TCTGGCCAAG GGTGGCGGCG TCGGAGGAGG GAAGATCATC 
AATGCCTCGC TGCGGCTCAA GTCACGCTTT CAGACCATGA GCATCCTGCC GATGGAACTG 
TATAAAGAGT TCGCAGAGTA CGTCACAAAC AACTACCGGA CGCTGTGTGC GGTCCTGGAG 
CCACTGCTGA GTGTCAAGAG CAAAGAAGAA GTGGCCTGTG CTCTGGTGCA CATCCTACAG 
AGCACAGGGA AGGCCAAGGA TTTTCTGTCA GACATGGCGA TGTGTGAGGT GGATCGCTTC 
ATCGACCGCG AGCACCTGAT CTTCAGAGAA AACACCCTGG CCACCAAAGC CATAGAAGAG 
TACCTCAAAC TCATCGGACA CAAGTACCTC AAAGATGTTT TAGGTGATTT TATCCGAGCG 
CTGTATGAAT CAGAGGAGAA CTGTGAAGTG GACCCGATGC GAACTCCCCC TTCAATACTG 
CCCGAACATC AAGCCAACCT CCGGATGTGC TGTGAGCTCG CCCTCTGCAA AATCATCAAC 
TCATATTGTG CGTTCCCGCG AGAGCTGAAG GAAGTGTTTG CATCATGGCG TGTCCGCTGT 
GCTGAGAGAG GAAGAGAAGA CATTGCAGAT CGACTGATCA GCGGCTCCCT CTTCCTGCGC 
TTCCTCTGTC CAGCCATCAT GTCTCCATCT CTGTTCAACC TCACGCAGGA ATATCCCGAT 
GAGCAGACCT CACGAACACT CACACTCATC GCCAAAGTGG TCCAGAACCT CGCAAACTTC 
TCCAAGTTTG GCAGTAAGGA GGAGTACATG TGCTTCATGA ATGAGTTTCT GGAGATGGAG 
TGGGGCTCCA TGCAGCAGTT TCTCTATGAG ATCTCCAATC TGGACAGTGT GAGCAATGCT 
GGGGCCTTCG AGGGCTACAT TGACCTAGGC CGCGAGCTCT CCATCCTGCA CAGTCTGCTC 
TGGGAGGTTA TGGCTCAGCT GAGCAAGTCA TCAATTAGGT CTTGGTTTAT TGTGTCAAAG 
GATGCCATCA TCAAACTTGG ACCATTGCCC CGCCTCCTCA ATGACATCAG CATGGCCCTC 
CGCAACCCTC ACCTGCAGAG GCAGCCCAGT CATCAGACAG ACAGACAGCC TGCAGAGAGA 
CAGACAGACC GACTGCTGTC CCGACCCAGC TTCAACAGGG GCGTCTCGTC TGAGTTCCAG 
AATCTCATGA TGAGAGACCT TAACAGTTCA GTGGAGATCA CTCGTCTGCC CTCTCCAACT 
TCTGCCATGT CCAGCGGTGG AGCTCCTCCA TCCCGTGCAG GAATGGCTGG GTTTGCGGAC 
CGCGATCATC CTCACCGAGC CTCGTCTAAA GATGTTTTCT ATGGTGCCCG TCCCCCACTG 
GCCCGCTCCA GTCCAGCGTA CTGTACTAGC AGTTCAGATA TTACTGATCC AGACCCAAAG 
GATTCCAGAA TGAACAGCGT CTCTAACCTG CAGTCTATGG GGGACATGCT CAACTCTTCC 
CAGGCCTCGA TTGCTGGCCT CGGCAGCTAC GGTGGGCTCG CAGGCTTGGG TGGAGGCCTC 
GGAGGTCAGC TGCGAGCCGG AGGACGTATG TCGGCCGGAT CTGGCGGTTC CAGTATGTCC 
GGGGGTCTGA GGTTGAGCCA GCTGAGCCAG ATGGGAACCA CTACCGACTC CCTGTCCCAG 
CAGCAGCAGC AGCAGGCCGC CGCCCTCCGC TTTCCCCTGT CCTTCCAGAA CCCTCTGTTC 
CACCTCGCTG CTGACGGACC GCAGCTTCAC CACCAGCACA GTCGAGCTCA GCCTCCTCCT 
CCGCTCCTGC TGGCCCCAGA GCCGGACGGT TCTCACCCTA CTTATATCCC ACAGTTTGCA 
CATGGTGGTT TCTCCCGAAG TGAGGACCTC TCCACCCTCA GACCCGGCCC TCATCTGGGC 
CAGCCAAGTA TTATTCATTC TCACAGCTAC AGCGACGACT ACAGCCGTCA TAACCAGAGC 
GACTACGGGC GACGACAGAT CTCCATGCAC ATGCAGGAGC AGCAGCAGAT GGCAGGCATG 
GCATCGCAGA CTGGCACATC TCATTCATCT CTCGCCACTC CTCCTTCCAC TGTTCAACCT 
GTTCGTCAAA GCTCCATGGC TCCTCCCACG CAGCGCATGA AATCACAACC CTCACATCAG 
CTTTCTGTGA GCTCTGCCGC TGGTGCCCCT GCGTCCAAAA CCAGGCCGCA GAGCGGAAAC 
CTGCTCCAGT CCCCGGAGTC TGGCTTCGGG GGCCGGCAGC AGGGCCCTCG GCAGCAGCTG 
TCTGTCAAAG ACAGCACCCC ACCCGGCCTG CCGCATCAAC AGAGCTCCAC CAGGGAGAGT 
CAGGGATCCC AGGGCTCTCA GGGTGGAACG CCGCAGTCCA CGCAGCAGTC CAAATCACAT 
CAGGAACGGC AGCAGGTCCA GCAACAGCAT CTGCTCAAGC CTACCATGAG CAAACAGGGC 
TCTTCACAAT CTCCATCCAC CCTGAACCCG CAGACCCCTG CCTCTGAGCG GACGGTCGCC 
TGGGTCTCCA ACATGCCCCA TCTTTCTGCT GACATAGAGA GTTCCCGCAT CGACAGAGAA 
GAGTTCAAGC TGAAGGAGTA CTCCAAGAGC ATGGACGAGT CCCGTCTGGA CCGGGTGCGC 
GAGTATGAGG AGGAGATCCA CTCACTGAAG GAGCGTCTGA TGATGTCCCA CCGCAAGTTG 
GAAGAGTACG AGAGGAGGCT GTTAACTCAG GAGCAGCAGA CCAATAAAAT CCTGCTGCAG 
TATCAGAGCC GACTGGACGA CAGCGAGAGG AGACTCCGAC AGCAGCAGAT GGAGAAAGAC 
AACCAAATTA AAGGAATTAT TGACAGACTC ATGGCGGTGG AGGACGAGTT GAGAGTAGGA 
GTGGTGCCTG AACACAAACC CAGAGTATTT GCGGATCAGG AAAAGCAGCT TTCCGCCTTG 
GGTTCAGCAG ACCCCGGTGT GAGTTTTGGA GGAGAAATGA CCAGCGCTTC TGGCTTTGTT 
GGAGTCTCCC CTTCGGACAA ATCCTCCACC CGATCCCCCC AGAATGGAAT CGCGTCCTTC 
CCGTTAGTTC CCCCTCCTCG TTCAATTCCT CACGCACAAC TCTGGCCGAA CGGCAACCAA 
AAATCTGCAA TGGATTGGAG GGCAAGTTTG GAGGGGGAGC AGGTCAATAA CAACGCTAAT 
CAAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290925.1
CDS	1449..5657
Translation	MEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKSSIRSWFIVSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRLMAVEDELRVGVVPEHKPRVFADQEKQLSALGSADPGVSFGGEMTSASGFVGVSPSDKSSTRSPQNGIASFPLVPPPRSIPHAQLWPNGNQKSAMDWRASLEGEQVNNNANQI

Target ORF information:

RefSeq Version	XM_009292650.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292650.2

ORF Insert Sequence:

ATGGAGATCC CACCCACTGC CCCCCCACAA CCCTTCCGAC AATCCGTGAG TAACCCACCT 
TTCCGTCCCC GGTCCCTCTC TCCATCCCTG TCCCTATCGC TGCCGTTCGC TCCATCGCCC 
CTGTCGCCCT CGCTGTCCCC GTCCCATTTA CAGTCCCAAA AGCGAATCAG CACTCCTCCG 
CTGCCTCGTT CTCTCCCTCT CTCCCCGAGC GTCTCTCTGA CTCTCCCACC CTCTGTCCCG 
CTCTCCCACT GGGCTGCTGG TGAGTGGAAG GTTGTGGGTT CTAATCCAGG GACGGGATCC 
AGTGTGGCGG CACTGCCCTC TCTTGAGAGT TTCCTCAGTA GAAGACTGAA GGGGTCCATT 
AAACGGGCCA AGAGTCAGCC CAAGCTGGAC CGAACCAGCA GCTTCCGCCA CATGATCCTC 
CCTCGCTTCC GCAGCGCCGA CCAGGAGCGG ACTCGTCTGA TGCAGAGCTT CAAAGAATCA 
CACTCCCATG AGTCTTTGCT GTCTCCCAGC AGCGCAGCCG AGGCCTTGGA TTTGACACTT 
GATGAAGACG CCATCATCAA GCCTGTTCAC TCCAGTATAT TGGGACAGGA GTACTGTTTC 
GAGGTGATAA CTGCTTCAGG AACAAAGTGT TTTGCCTGTC GGTCGGCTGC GGAGCGAGAC 
AAATGGATCG AGAATCTACA GAGAGCTGTC AAACCCAACA AAGACAACAG TCGGCGTGTC 
GACAACGTCC TGAAGCTGTG GATCATTGAG GCCCGTGAGC TCCCACCCAA AAAGCGCTAT 
TATTGTGAAC TGTGCTTGGA TGACATGCTG TACGCTCGCA CCACCAGCAA ACCCCGTACC 
GACACTGTCT TCTGGGGTGA GCACTTTGAG TTCAACAACC TGCCTGCTGT CCGCAACCTC 
CGCCTTCATC TGTACAAAGA GACGGACAAG AAGAGACGCA AGGAGAAGAG CACGTACCTT 
GGCTTGGTCA GCATTCCCAT TTCCAGCATC ACTGGCCGTC AGTTTGTGGA GCAATGGTAC 
CCCGTCATTC AGCCCAGTGT TCTGGCCAAG GGTGGCGGCG TCGGAGGAGG GAAGATCATC 
AATGCCTCGC TGCGGCTCAA GTCACGCTTT CAGACCATGA GCATCCTGCC GATGGAACTG 
TATAAAGAGT TCGCAGAGTA CGTCACAAAC AACTACCGGA CGCTGTGTGC GGTCCTGGAG 
CCACTGCTGA GTGTCAAGAG CAAAGAAGAA GTGGCCTGTG CTCTGGTGCA CATCCTACAG 
AGCACAGGGA AGGCCAAGGA TTTTCTGTCA GACATGGCGA TGTGTGAGGT GGATCGCTTC 
ATCGACCGCG AGCACCTGAT CTTCAGAGAA AACACCCTGG CCACCAAAGC CATAGAAGAG 
TACCTCAAAC TCATCGGACA CAAGTACCTC AAAGATGTTT TAGGTGATTT TATCCGAGCG 
CTGTATGAAT CAGAGGAGAA CTGTGAAGTG GACCCGATGC GAACTCCCCC TTCAATACTG 
CCCGAACATC AAGCCAACCT CCGGATGTGC TGTGAGCTCG CCCTCTGCAA AATCATCAAC 
TCATATTGTG CGTTCCCGCG AGAGCTGAAG GAAGTGTTTG CATCATGGCG TGTCCGCTGT 
GCTGAGAGAG GAAGAGAAGA CATTGCAGAT CGACTGATCA GCGGCTCCCT CTTCCTGCGC 
TTCCTCTGTC CAGCCATCAT GTCTCCATCT CTGTTCAACC TCACGCAGGA ATATCCCGAT 
GAGCAGACCT CACGAACACT CACACTCATC GCCAAAGTGG TCCAGAACCT CGCAAACTTC 
TCCAAGTTTG GCAGTAAGGA GGAGTACATG TGCTTCATGA ATGAGTTTCT GGAGATGGAG 
TGGGGCTCCA TGCAGCAGTT TCTCTATGAG ATCTCCAATC TGGACAGTGT GAGCAATGCT 
GGGGCCTTCG AGGGCTACAT TGACCTAGGC CGCGAGCTCT CCATCCTGCA CAGTCTGCTC 
TGGGAGGTTA TGGCTCAGCT GAGCAAGTCA TCAATTAGGT CTTGGTTTAT TGTGTCAAAG 
GATGCCATCA TCAAACTTGG ACCATTGCCC CGCCTCCTCA ATGACATCAG CATGGCCCTC 
CGCAACCCTC ACCTGCAGAG GCAGCCCAGT CATCAGACAG ACAGACAGCC TGCAGAGAGA 
CAGACAGACC GACTGCTGTC CCGACCCAGC TTCAACAGGG GCGTCTCGTC TGAGTTCCAG 
AATCTCATGA TGAGAGACCT TAACAGTTCA GTGGAGATCA CTCGTCTGCC CTCTCCAACT 
TCTGCCATGT CCAGCGGTGG AGCTCCTCCA TCCCGTGCAG GAATGGCTGG GTTTGCGGAC 
CGCGATCATC CTCACCGAGC CTCGTCTAAA GATGTTTTCT ATGGTGCCCG TCCCCCACTG 
GCCCGCTCCA GTCCAGCGTA CTGTACTAGC AGTTCAGATA TTACTGATCC AGACCCAAAG 
GATTCCAGAA TGAACAGCGT CTCTAACCTG CAGTCTATGG GGGACATGCT CAACTCTTCC 
CAGGCCTCGA TTGCTGGCCT CGGCAGCTAC GGTGGGCTCG CAGGCTTGGG TGGAGGCCTC 
GGAGGTCAGC TGCGAGCCGG AGGACGTATG TCGGCCGGAT CTGGCGGTTC CAGTATGTCC 
GGGGGTCTGA GGTTGAGCCA GCTGAGCCAG ATGGGAACCA CTACCGACTC CCTGTCCCAG 
CAGCAGCAGC AGCAGGCCGC CGCCCTCCGC TTTCCCCTGT CCTTCCAGAA CCCTCTGTTC 
CACCTCGCTG CTGACGGACC GCAGCTTCAC CACCAGCACA GTCGAGCTCA GCCTCCTCCT 
CCGCTCCTGC TGGCCCCAGA GCCGGACGGT TCTCACCCTA CTTATATCCC ACAGTTTGCA 
CATGGTGGTT TCTCCCGAAG TGAGGACCTC TCCACCCTCA GACCCGGCCC TCATCTGGGC 
CAGCCAAGTA TTATTCATTC TCACAGCTAC AGCGACGACT ACAGCCGTCA TAACCAGAGC 
GACTACGGGC GACGACAGAT CTCCATGCAC ATGCAGGAGC AGCAGCAGAT GGCAGGCATG 
GCATCGCAGA CTGGCACATC TCATTCATCT CTCGCCACTC CTCCTTCCAC TGTTCAACCT 
GTTCGTCAAA GCTCCATGGC TCCTCCCACG CAGCGCATGA AATCACAACC CTCACATCAG 
CTTTCTGTGA GCTCTGCCGC TGGTGCCCCT GCGTCCAAAA CCAGGCCGCA GAGCGGAAAC 
CTGCTCCAGT CCCCGGAGTC TGGCTTCGGG GGCCGGCAGC AGGGCCCTCG GCAGCAGCTG 
TCTGTCAAAG ACAGCACCCC ACCCGGCCTG CCGCATCAAC AGAGCTCCAC CAGGGAGAGT 
CAGGGATCCC AGGGCTCTCA GGGTGGAACG CCGCAGTCCA CGCAGCAGTC CAAATCACAT 
CAGGAACGGC AGCAGGTCCA GCAACAGCAT CTGCTCAAGC CTACCATGAG CAAACAGGGC 
TCTTCACAAT CTCCATCCAC CCTGAACCCG CAGACCCCTG CCTCTGAGCG GACGGTCGCC 
TGGGTCTCCA ACATGCCCCA TCTTTCTGCT GACATAGAGA GTTCCCGCAT CGACAGAGAA 
GAGTTCAAGC TGAAGGAGTA CTCCAAGAGC ATGGACGAGT CCCGTCTGGA CCGGGTGCGC 
GAGTATGAGG AGGAGATCCA CTCACTGAAG GAGCGTCTGA TGATGTCCCA CCGCAAGTTG 
GAAGAGTACG AGAGGAGGCT GTTAACTCAG GAGCAGCAGA CCAATAAAAT CCTGCTGCAG 
TATCAGAGCC GACTGGACGA CAGCGAGAGG AGACTCCGAC AGCAGCAGAT GGAGAAAGAC 
AACCAAATTA AAGGAATTAT TGACAGACTC ATGGCGGTGG AGGACGAGTT GAGAGTAGGA 
GTGGTGCCTG AACACAAACC CAGAGTATTT GCGGATCAGG AAAAGCAGCT TTCCGCCTTG 
GGTTCAGCAG ACCCCGGTGT GAGTTTTGGA GGAGAAATGA CCAGCGCTTC TGGCTTTGTT 
GGAGTCTCCC CTTCGGACAA ATCCTCCACC CGATCCCCCC AGAATGGAAT CGCGTCCTTC 
CCGTTAGTTC CCCCTCCTCG TTCAATTCCT CACGCACAAC TCTGGCCGAA CGGCAACCAA 
AAATCTGCAA TGGATTGGAG GGCAAGTTTG GAGGGGGAGC AGGTCAATAA CAACGCTAAT 
CAAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syngap1b ( XM_017351306.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206795.1 Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X10, mRNA.

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CloneID	ODa55357
Clone ID Related Accession (Same CDS sequence)	XM_017351306.1
Accession Version	XM_017351306.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	si:dkey-246f6.1 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGATTC CAGAATGAAC AGCGTCTCTA ACCTGCAGTC TATGGGGGAC 
ATGCTCAACT CTTCCCAGGC CTCGATTGCT GGCCTCGGCA GCTACGGTGG GCTCGCAGGC 
TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA GCCGGAGGAC GTATGTCGGC CGGATCTGGC 
GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG AGCCAGCTGA GCCAGATGGG AACCACTACC 
GACTCCCTGT CCCAGCAGCA GCAGCAGCAG GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC 
CAGAACCCTC TGTTCCACCT CGCTGCTGAC GGACCGCAGC TTCACCACCA GCACAGTCGA 
GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC CCAGAGCCGG ACGGTTCTCA CCCTACTTAT 
ATCCCACAGT TTGCACATGG TGGTTTCTCC CGAAGTGAGG ACCTCTCCAC CCTCAGACCC 
GGCCCTCATC TGGGCCAGCC AAGTATTATT CATTCTCACA GCTACAGCGA CGACTACAGC 
CGTCATAACC AGAGCGACTA CGGGCGACGA CAGATCTCCA TGCACATGCA GGAGCAGCAG 
CAGATGGCAG GCATGGCATC GCAGACTGGC ACATCTCATT CATCTCTCGC CACTCCTCCT 
TCCACTGTTC AACCTGTTCG TCAAAGCTCC ATGGCTCCTC CCACGCAGCG CATGAAATCA 
CAACCCTCAC ATCAGCTTTC TGTGAGCTCT GCCGCTGGTG CCCCTGCGTC CAAAACCAGG 
CCGCAGAGCG GAAACCTGCT CCAGTCCCCG GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC 
CCTCGGCAGC AGCTGTCTGT CAAAGACAGC ACCCCACCCG GCCTGCCGCA TCAACAGAGC 
TCCACCAGGG AGAGTCAGGG ATCCCAGGGC TCTCAGGGTG GAACGCCGCA GTCCACGCAG 
CAGTCCAAAT CACATCAGGA ACGGCAGCAG GTCCAGCAAC AGCATCTGCT CAAGCCTACC 
ATGAGCAAAC AGGGCTCTTC ACAATCTCCA TCCACCCTGA ACCCGCAGAC CCCTGCCTCT 
GAGCGGACGG TCGCCTGGGT CTCCAACATG CCCCATCTTT CTGCTGACAT AGAGAGTTCC 
CGCATCGACA GAGAAGAGTT CAAGCTGAAG GAGTACTCCA AGAGCATGGA CGAGTCCCGT 
CTGGACCGGG TGCGCGAGTA TGAGGAGGAG ATCCACTCAC TGAAGGAGCG TCTGATGATG 
TCCCACCGCA AGTTGGAAGA GTACGAGAGG AGGCTGTTAA CTCAGGAGCA GCAGACCAAT 
AAAATCCTGC TGCAGTATCA GAGCCGACTG GACGACAGCG AGAGGAGACT CCGACAGCAG 
CAGATGGAGA AAGACAACCA AATTAAAGGA ATTATTGACA GGAATGAAGA GATGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206795.1
CDS	316..4515
Translation	MSYVPFQDARYAVPPSYRPQPSFAAAPSYEQADWNPRLGVISGNQLYMLDQEEVHPLLMRERRSESHRNKLLRRTVSVPVEARHHPEMDAARLRRKSIATGKQPSMEIPPTAPPQPFRQSVSNPPFRPRSLSPSLSLSLPFAPSPLSPSLSPSHLQSQKRISTPPLPRSLPLSPSVSLTLPPSVPLSHWAAGEWKVVGSNPGTGSSVAALPSLESFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQERTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVITASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGGGVGGGKIINASLRLKSRFQTMSILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDVLGDFIRALYESEENCEVDPMRTPPSILPEHQANLRMCCELALCKIINSYCAFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGAFEGYIDLGRELSILHSLLWEVMAQLSKSSIRSWFIVSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRQPAERQTDRLLSRPSFNRGVSSEFQNLMMRDLNSSVEITRLPSPTSAMSSGGAPPSRAGMAGFADRDHPHRASSKDVFYGARPPLARSSPAYCTSSSDITDPDPKDSRMNSVSNLQSMGDMLNSSQASIAGLGSYGGLAGLGGGLGGQLRAGGRMSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRFPLSFQNPLFHLAADGPQLHHQHSRAQPPPPLLLAPEPDGSHPTYIPQFAHGGFSRSEDLSTLRPGPHLGQPSIIHSHSYSDDYSRHNQSDYGRRQISMHMQEQQQMAGMASQTGTSHSSLATPPSTVQPVRQSSMAPPTQRMKSQPSHQLSVSSAAGAPASKTRPQSGNLLQSPESGFGGRQQGPRQQLSVKDSTPPGLPHQQSSTRESQGSQGSQGGTPQSTQQSKSHQERQQVQQQHLLKPTMSKQGSSQSPSTLNPQTPASERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRLDRVREYEEEIHSLKERLMMSHRKLEEYERRLLTQEQQTNKILLQYQSRLDDSERRLRQQQMEKDNQIKGIIDRNEEME

Target ORF information:

RefSeq Version	XM_017351306.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio synaptic Ras GTPase activating protein 1b (syngap1b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351306.1

ORF Insert Sequence:

ATGTCATATG TTCCCTTTCA AGATGCGAGG TATGCCGTTC CCCCGTCCTA CAGACCCCAG 
CCGTCGTTCG CTGCTGCTCC GTCATACGAG CAGGCCGACT GGAATCCTCG TCTGGGTGTG 
ATCAGTGGAA ATCAGCTCTA CATGTTGGAC CAGGAGGAGG TTCACCCTTT GTTGATGCGC 
GAGCGGCGCT CCGAGTCGCA CAGGAACAAG CTGCTGCGAC GGACGGTCAG TGTGCCTGTG 
GAGGCCAGGC ACCATCCTGA AATGGACGCC GCTCGGCTCC GGCGGAAAAG CATCGCCACC 
GGGAAGCAGC CAAGCATGGA GATCCCACCC ACTGCCCCCC CACAACCCTT CCGACAATCC 
GTGAGTAACC CACCTTTCCG TCCCCGGTCC CTCTCTCCAT CCCTGTCCCT ATCGCTGCCG 
TTCGCTCCAT CGCCCCTGTC GCCCTCGCTG TCCCCGTCCC ATTTACAGTC CCAAAAGCGA 
ATCAGCACTC CTCCGCTGCC TCGTTCTCTC CCTCTCTCCC CGAGCGTCTC TCTGACTCTC 
CCACCCTCTG TCCCGCTCTC CCACTGGGCT GCTGGTGAGT GGAAGGTTGT GGGTTCTAAT 
CCAGGGACGG GATCCAGTGT GGCGGCACTG CCCTCTCTTG AGAGTTTCCT CAGTAGAAGA 
CTGAAGGGGT CCATTAAACG GGCCAAGAGT CAGCCCAAGC TGGACCGAAC CAGCAGCTTC 
CGCCACATGA TCCTCCCTCG CTTCCGCAGC GCCGACCAGG AGCGGACTCG TCTGATGCAG 
AGCTTCAAAG AATCACACTC CCATGAGTCT TTGCTGTCTC CCAGCAGCGC AGCCGAGGCC 
TTGGATTTGA CACTTGATGA AGACGCCATC ATCAAGCCTG TTCACTCCAG TATATTGGGA 
CAGGAGTACT GTTTCGAGGT GATAACTGCT TCAGGAACAA AGTGTTTTGC CTGTCGGTCG 
GCTGCGGAGC GAGACAAATG GATCGAGAAT CTACAGAGAG CTGTCAAACC CAACAAAGAC 
AACAGTCGGC GTGTCGACAA CGTCCTGAAG CTGTGGATCA TTGAGGCCCG TGAGCTCCCA 
CCCAAAAAGC GCTATTATTG TGAACTGTGC TTGGATGACA TGCTGTACGC TCGCACCACC 
AGCAAACCCC GTACCGACAC TGTCTTCTGG GGTGAGCACT TTGAGTTCAA CAACCTGCCT 
GCTGTCCGCA ACCTCCGCCT TCATCTGTAC AAAGAGACGG ACAAGAAGAG ACGCAAGGAG 
AAGAGCACGT ACCTTGGCTT GGTCAGCATT CCCATTTCCA GCATCACTGG CCGTCAGTTT 
GTGGAGCAAT GGTACCCCGT CATTCAGCCC AGTGTTCTGG CCAAGGGTGG CGGCGTCGGA 
GGAGGGAAGA TCATCAATGC CTCGCTGCGG CTCAAGTCAC GCTTTCAGAC CATGAGCATC 
CTGCCGATGG AACTGTATAA AGAGTTCGCA GAGTACGTCA CAAACAACTA CCGGACGCTG 
TGTGCGGTCC TGGAGCCACT GCTGAGTGTC AAGAGCAAAG AAGAAGTGGC CTGTGCTCTG 
GTGCACATCC TACAGAGCAC AGGGAAGGCC AAGGATTTTC TGTCAGACAT GGCGATGTGT 
GAGGTGGATC GCTTCATCGA CCGCGAGCAC CTGATCTTCA GAGAAAACAC CCTGGCCACC 
AAAGCCATAG AAGAGTACCT CAAACTCATC GGACACAAGT ACCTCAAAGA TGTTTTAGGT 
GATTTTATCC GAGCGCTGTA TGAATCAGAG GAGAACTGTG AAGTGGACCC GATGCGAACT 
CCCCCTTCAA TACTGCCCGA ACATCAAGCC AACCTCCGGA TGTGCTGTGA GCTCGCCCTC 
TGCAAAATCA TCAACTCATA TTGTGCGTTC CCGCGAGAGC TGAAGGAAGT GTTTGCATCA 
TGGCGTGTCC GCTGTGCTGA GAGAGGAAGA GAAGACATTG CAGATCGACT GATCAGCGGC 
TCCCTCTTCC TGCGCTTCCT CTGTCCAGCC ATCATGTCTC CATCTCTGTT CAACCTCACG 
CAGGAATATC CCGATGAGCA GACCTCACGA ACACTCACAC TCATCGCCAA AGTGGTCCAG 
AACCTCGCAA ACTTCTCCAA GTTTGGCAGT AAGGAGGAGT ACATGTGCTT CATGAATGAG 
TTTCTGGAGA TGGAGTGGGG CTCCATGCAG CAGTTTCTCT ATGAGATCTC CAATCTGGAC 
AGTGTGAGCA ATGCTGGGGC CTTCGAGGGC TACATTGACC TAGGCCGCGA GCTCTCCATC 
CTGCACAGTC TGCTCTGGGA GGTTATGGCT CAGCTGAGCA AGTCATCAAT TAGGTCTTGG 
TTTATTGTGT CAAAGGATGC CATCATCAAA CTTGGACCAT TGCCCCGCCT CCTCAATGAC 
ATCAGCATGG CCCTCCGCAA CCCTCACCTG CAGAGGCAGC CCAGTCATCA GACAGACAGA 
CAGCCTGCAG AGAGACAGAC AGACCGACTG CTGTCCCGAC CCAGCTTCAA CAGGGGCGTC 
TCGTCTGAGT TCCAGAATCT CATGATGAGA GACCTTAACA GTTCAGTGGA GATCACTCGT 
CTGCCCTCTC CAACTTCTGC CATGTCCAGC GGTGGAGCTC CTCCATCCCG TGCAGGAATG 
GCTGGGTTTG CGGACCGCGA TCATCCTCAC CGAGCCTCGT CTAAAGATGT TTTCTATGGT 
GCCCGTCCCC CACTGGCCCG CTCCAGTCCA GCGTACTGTA CTAGCAGTTC AGATATTACT 
GATCCAGACC CAAAGGATTC CAGAATGAAC AGCGTCTCTA ACCTGCAGTC TATGGGGGAC 
ATGCTCAACT CTTCCCAGGC CTCGATTGCT GGCCTCGGCA GCTACGGTGG GCTCGCAGGC 
TTGGGTGGAG GCCTCGGAGG TCAGCTGCGA GCCGGAGGAC GTATGTCGGC CGGATCTGGC 
GGTTCCAGTA TGTCCGGGGG TCTGAGGTTG AGCCAGCTGA GCCAGATGGG AACCACTACC 
GACTCCCTGT CCCAGCAGCA GCAGCAGCAG GCCGCCGCCC TCCGCTTTCC CCTGTCCTTC 
CAGAACCCTC TGTTCCACCT CGCTGCTGAC GGACCGCAGC TTCACCACCA GCACAGTCGA 
GCTCAGCCTC CTCCTCCGCT CCTGCTGGCC CCAGAGCCGG ACGGTTCTCA CCCTACTTAT 
ATCCCACAGT TTGCACATGG TGGTTTCTCC CGAAGTGAGG ACCTCTCCAC CCTCAGACCC 
GGCCCTCATC TGGGCCAGCC AAGTATTATT CATTCTCACA GCTACAGCGA CGACTACAGC 
CGTCATAACC AGAGCGACTA CGGGCGACGA CAGATCTCCA TGCACATGCA GGAGCAGCAG 
CAGATGGCAG GCATGGCATC GCAGACTGGC ACATCTCATT CATCTCTCGC CACTCCTCCT 
TCCACTGTTC AACCTGTTCG TCAAAGCTCC ATGGCTCCTC CCACGCAGCG CATGAAATCA 
CAACCCTCAC ATCAGCTTTC TGTGAGCTCT GCCGCTGGTG CCCCTGCGTC CAAAACCAGG 
CCGCAGAGCG GAAACCTGCT CCAGTCCCCG GAGTCTGGCT TCGGGGGCCG GCAGCAGGGC 
CCTCGGCAGC AGCTGTCTGT CAAAGACAGC ACCCCACCCG GCCTGCCGCA TCAACAGAGC 
TCCACCAGGG AGAGTCAGGG ATCCCAGGGC TCTCAGGGTG GAACGCCGCA GTCCACGCAG 
CAGTCCAAAT CACATCAGGA ACGGCAGCAG GTCCAGCAAC AGCATCTGCT CAAGCCTACC 
ATGAGCAAAC AGGGCTCTTC ACAATCTCCA TCCACCCTGA ACCCGCAGAC CCCTGCCTCT 
GAGCGGACGG TCGCCTGGGT CTCCAACATG CCCCATCTTT CTGCTGACAT AGAGAGTTCC 
CGCATCGACA GAGAAGAGTT CAAGCTGAAG GAGTACTCCA AGAGCATGGA CGAGTCCCGT 
CTGGACCGGG TGCGCGAGTA TGAGGAGGAG ATCCACTCAC TGAAGGAGCG TCTGATGATG 
TCCCACCGCA AGTTGGAAGA GTACGAGAGG AGGCTGTTAA CTCAGGAGCA GCAGACCAAT 
AAAATCCTGC TGCAGTATCA GAGCCGACTG GACGACAGCG AGAGGAGACT CCGACAGCAG 
CAGATGGAGA AAGACAACCA AATTAAAGGA ATTATTGACA GGAATGAAGA GATGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Two knockdown models of the autism genes SYNGAP1 and SHANK3 in zebrafish produce similar behavioral phenotypes associated with embryonic disruptions of brain morphogenesis.
Human molecular genetics24(14)4006-23(2015 Jul)
Kozol RA,Cukier HN,Zou B,Mayo V,De Rubeis S,Cai G,Griswold AJ,Whitehead PL,Haines JL,Gilbert JR,Cuccaro ML,Martin ER,Baker JD,Buxbaum JD,Pericak-Vance MA,Dallman JE

Evolutionary history of the ABCB2 genomic region in teleosts.
Developmental and comparative immunology31(5)483-98(2007)
Palti Y,Rodriguez MF,Gahr SA,Hansen JD

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syngap1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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