mmp21 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mmp21
Entrez Gene ID	100003089
Full Name	matrix metallopeptidase 21
Synonyms	MMP-21
General protein information	Preferred Names matrix metallopeptidase 21 Names matrix metallopeptidase-21 matrix metalloproteinase-21
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001317753.1	NP_001304682.1	matrix metallopeptidase-21 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MMP21	XP_003312866.1
	MMP21	XP_001085506.1
Danio rerio (zebrafish)	LOC100003089	XP_001342721.4
Rattus norvegicus (Norway rat)	Mmp21	NP_001099778.1
Xenopus tropicalis (tropical clawed frog)	mmp21	XP_002932698.2
Homo sapiens (human)	MMP21	NP_671724.1
Mus musculus (house mouse)	Mmp21	NP_694423.1
Canis lupus familiaris (dog)	MMP21	XP_003640036.1

Gene Ontology
Bibliography

Related articles in PubMed

Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy.
Colin E, Daniel J, Ziegler A, Wakim J, Scrivo A, Haack TB, Khiati S, Denomm? AS, Amati-Bonneau P, Charif M, Procaccio V, Reynier P, Aleck KA, Botto LD, Herper CL, Kaiser CS, Nabbout R, N'Guyen S, Mora-Lorca JA, Assmann B, Christ S, Meitinger T, Strom TM, Prokisch H, , Miranda-Vizuete A, Hoffmann GF, Lenaers G, Bomont P, Liebau E, Bonneau D
American journal of human genetics99(3)695-703(2016 Sep)

A human laterality disorder caused by a homozygous deleterious mutation in MMP21.
Perles Z, Moon S, Ta-Shma A, Yaacov B, Francescatto L, Edvardson S, Rein AJ, Elpeleg O, Katsanis N
Journal of medical genetics52(12)840-7(2015 Dec)

MMP21 is mutated in human heterotaxy and is required for normal left-right asymmetry in vertebrates.
Guimier A, Gabriel GC, Bajolle F, Tsang M, Liu H, Noll A, Schwartz M, El Malti R, Smith LD, Klena NT, Jimenez G, Miller NA, Oufadem M, Moreau de Bellaing A, Yagi H, Saunders CJ, Baker CN, Di Filippo S, Peterson KA, Thiffault I, Bole-Feysot C, Cooley LD, Farrow EG, Masson C, Schoen P, Deleuze JF, Nitschk? P, Lyonnet S, de Pontual L, Murray SA, Bonnet D, Kingsmore SF, Amiel J, Bouvagnet P, Lo CW, Gordon CT
Nature genetics47(11)1260-3(2015 Nov)

The zebrafish embryo: a powerful model system for investigating matrix remodeling.
Wyatt RA, Keow JY, Harris ND, Hach? CA, Li DH, Crawford BD
Zebrafish6(4)347-54(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mmp21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mmp21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa98504	NM_001317753.1 Latest version!	Danio rerio matrix metallopeptidase 21 (mmp21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mmp21 ( NM_001317753.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001304682.1 Danio rerio matrix metallopeptidase 21 (mmp21), mRNA.

Price & Availability↑

CloneID	ODa98504
Clone ID Related Accession (Same CDS sequence)	NM_001317753.1
Accession Version	NM_001317753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Danio rerio(zebrafish)
Product	matrix metallopeptidase-21 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KT207790.1. On Dec 8, 2015 this sequence version replaced XM_001342685.6. ##Evidence-Data-START## Transcript exon combination :: KT207790.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGCTGACTG TTATTCGGCG GATCTTCATC ATCCAGACCT TCATCTTCAT CACAGCGGAG 
AAGATCTTCC ACAGCAGAGA CCATTCAGAT GTGCTGAACA ACATTCATCA GGCGGAGCTG 
ATCACTGACA CGGACACTGC ACAGCGTTTT CTGTCCAAGT ATGGCTTCAT CAAGGCAGCA 
GGCAGTGAAG AGTCTCAGCT GTCAGAATCT TCAGGTGATC TGGACTTCAG TCTCTCTCTG 
GATCTCCATG AAGGAGGAAC CACATCCGGC TCCAGCAGCT CTGATCTGCA GTTTGTCTCT 
GCGCTGAGGG ATTTCCAGCG GCTCTCAGAT CTGCCCGTCA CCGGAGTGTT TGACGACGCC 
ACCAAAGCAG CCATGAATAA ACCAAGATGT GGCGTTATGG ATGACGATCA AGAACTGAAG 
GATGTCACTG GATCCAACAG CACCAGGAAT CACATCAGAA CTAGCACCAA TACATCCCAT 
AATCATGAGC ATCAGGCTCC GGTGCGCAAG AAGCGTCACC TGTCTGCTCT TCTGAAGAAT 
ACAAAGCTGC AGAAGCGAGA TGTGAGCGAG TGGACGGGAC ACATGGCCTT CTCCAAAAGT 
GTGCTGAAGT GGCGTCTGAT TGGTGAAGGA TACAGCAGCC AGCTCTCCAT CCAGGAGCAG 
AAATACATCT TCAGACTGGC CTTCAGAATG TGGAGCGAAA TCTCACCGCT GCAGTTCATC 
GAAGATCTGC ACTCGCCCCT GGGAAACATC GACATCCGGC TGGGCTTCGG CACGGGTCGT 
CATCTGGGCT GCTCTCAGCG GTTTGACGGC GCTGGTCGAG AGTTCGCTCA TGCCTGGTTC 
CTCGGAGACA TTCATTTCGA CGATGATGAA CATTTCACCG TCCCAAACAC AGGGAGCGGC 
ATCAGTCTGC TTAAGGTAGC GGTTCATGAG ATCGGCCATG TTTTAGGGCT TCCTCACATT 
TACCGTCCCG GATCCATCAT GCAGCCCAGT TACCTTCCTC AGGATGCCGG CTTCGAAATC 
GATTGGATGG ACCGCAAATC TATCCAGAGG CTGTACGGAG TGTGTATGGG TCGCTTCAGC 
ACCGTGTTTG ATTGGATCAG GAAGGAGCAG ACGCCGTACG GTGAGGTGGT GGTGCGTTTT 
AACACATACT TCATGCGGGA CGGCCTGTAC TGGCTCTACG AGAACCGCAA TAACAGGACG 
CGTTACGGGG ACCCAGTGGC GGTGCAGGTG GGCTGGCACG GGCTGCCCTC TGGGGGTGTG 
GATGCTTATG TGCACGTCTG GAACAGAAAA ACAGATGCTG TCTACTTCTT TAAAGGTATG 
CAGTACTGGC GTTATGACAG TGAGAATGAT CATGTTTTCA GTCATGCTCC AGACGGACGC 
TCGTATCCTC GTCTGATCTC TGAAGATTTC CCCGGTGTAT CTGGACCGCT GGACACGGCG 
TACTACGACA GAAGAGACGC TCACATCTAC TTCTTCAAAG GCTCACAGGT GTTCAGGTTT 
GATGTGCGCA TGCGCCGCTT GGCTTCTTCT TCTCCACAAG AGATGACGGA GGTTTTTCCA 
GCCATAGTGT CCGGTGATCA TCCTGTGAGG AGTCTGGACG CCGCGTATTT CTCCTACACA 
CACAACACAG TGTTCCTGCT GAAGGGCTCG CTGTTCTGGA GAGTGTTGAG CGGGAAAGAG 
CGCAGGCGGA GGGCTTTTCT CCCGATGAAC GGCCTGCTGG CTCACAGGCG CGTGCATGAG 
CAGTGGTTCG ACATCTGTGA CGTTCACAGC TCATCTCTGA GGACCACGCG CAGGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304682.1
CDS	62..1861
Translation	MLTVIRRIFIIQTFIFITAEKIFHSRDHSDVLNNIHQAELITDTDTAQRFLSKYGFIKAAGSEESQLSESSGDLDFSLSLDLHEGGTTSGSSSSDLQFVSALRDFQRLSDLPVTGVFDDATKAAMNKPRCGVMDDDQELKDVTGSNSTRNHIRTSTNTSHNHEHQAPVRKKRHLSALLKNTKLQKRDVSEWTGHMAFSKSVLKWRLIGEGYSSQLSIQEQKYIFRLAFRMWSEISPLQFIEDLHSPLGNIDIRLGFGTGRHLGCSQRFDGAGREFAHAWFLGDIHFDDDEHFTVPNTGSGISLLKVAVHEIGHVLGLPHIYRPGSIMQPSYLPQDAGFEIDWMDRKSIQRLYGVCMGRFSTVFDWIRKEQTPYGEVVVRFNTYFMRDGLYWLYENRNNRTRYGDPVAVQVGWHGLPSGGVDAYVHVWNRKTDAVYFFKGMQYWRYDSENDHVFSHAPDGRSYPRLISEDFPGVSGPLDTAYYDRRDAHIYFFKGSQVFRFDVRMRRLASSSPQEMTEVFPAIVSGDHPVRSLDAAYFSYTHNTVFLLKGSLFWRVLSGKERRRRAFLPMNGLLAHRRVHEQWFDICDVHSSSLRTTRRR

Target ORF information:

RefSeq Version	NM_001317753.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio matrix metallopeptidase 21 (mmp21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317753.1

ORF Insert Sequence:

ATGCTGACTG TTATTCGGCG GATCTTCATC ATCCAGACCT TCATCTTCAT CACAGCGGAG 
AAGATCTTCC ACAGCAGAGA CCATTCAGAT GTGCTGAACA ACATTCATCA GGCGGAGCTG 
ATCACTGACA CGGACACTGC ACAGCGTTTT CTGTCCAAGT ATGGCTTCAT CAAGGCAGCA 
GGCAGTGAAG AGTCTCAGCT GTCAGAATCT TCAGGTGATC TGGACTTCAG TCTCTCTCTG 
GATCTCCATG AAGGAGGAAC CACATCCGGC TCCAGCAGCT CTGATCTGCA GTTTGTCTCT 
GCGCTGAGGG ATTTCCAGCG GCTCTCAGAT CTGCCCGTCA CCGGAGTGTT TGACGACGCC 
ACCAAAGCAG CCATGAATAA ACCAAGATGT GGCGTTATGG ATGACGATCA AGAACTGAAG 
GATGTCACTG GATCCAACAG CACCAGGAAT CACATCAGAA CTAGCACCAA TACATCCCAT 
AATCATGAGC ATCAGGCTCC GGTGCGCAAG AAGCGTCACC TGTCTGCTCT TCTGAAGAAT 
ACAAAGCTGC AGAAGCGAGA TGTGAGCGAG TGGACGGGAC ACATGGCCTT CTCCAAAAGT 
GTGCTGAAGT GGCGTCTGAT TGGTGAAGGA TACAGCAGCC AGCTCTCCAT CCAGGAGCAG 
AAATACATCT TCAGACTGGC CTTCAGAATG TGGAGCGAAA TCTCACCGCT GCAGTTCATC 
GAAGATCTGC ACTCGCCCCT GGGAAACATC GACATCCGGC TGGGCTTCGG CACGGGTCGT 
CATCTGGGCT GCTCTCAGCG GTTTGACGGC GCTGGTCGAG AGTTCGCTCA TGCCTGGTTC 
CTCGGAGACA TTCATTTCGA CGATGATGAA CATTTCACCG TCCCAAACAC AGGGAGCGGC 
ATCAGTCTGC TTAAGGTAGC GGTTCATGAG ATCGGCCATG TTTTAGGGCT TCCTCACATT 
TACCGTCCCG GATCCATCAT GCAGCCCAGT TACCTTCCTC AGGATGCCGG CTTCGAAATC 
GATTGGATGG ACCGCAAATC TATCCAGAGG CTGTACGGAG TGTGTATGGG TCGCTTCAGC 
ACCGTGTTTG ATTGGATCAG GAAGGAGCAG ACGCCGTACG GTGAGGTGGT GGTGCGTTTT 
AACACATACT TCATGCGGGA CGGCCTGTAC TGGCTCTACG AGAACCGCAA TAACAGGACG 
CGTTACGGGG ACCCAGTGGC GGTGCAGGTG GGCTGGCACG GGCTGCCCTC TGGGGGTGTG 
GATGCTTATG TGCACGTCTG GAACAGAAAA ACAGATGCTG TCTACTTCTT TAAAGGTATG 
CAGTACTGGC GTTATGACAG TGAGAATGAT CATGTTTTCA GTCATGCTCC AGACGGACGC 
TCGTATCCTC GTCTGATCTC TGAAGATTTC CCCGGTGTAT CTGGACCGCT GGACACGGCG 
TACTACGACA GAAGAGACGC TCACATCTAC TTCTTCAAAG GCTCACAGGT GTTCAGGTTT 
GATGTGCGCA TGCGCCGCTT GGCTTCTTCT TCTCCACAAG AGATGACGGA GGTTTTTCCA 
GCCATAGTGT CCGGTGATCA TCCTGTGAGG AGTCTGGACG CCGCGTATTT CTCCTACACA 
CACAACACAG TGTTCCTGCT GAAGGGCTCG CTGTTCTGGA GAGTGTTGAG CGGGAAAGAG 
CGCAGGCGGA GGGCTTTTCT CCCGATGAAC GGCCTGCTGG CTCACAGGCG CGTGCATGAG 
CAGTGGTTCG ACATCTGTGA CGTTCACAGC TCATCTCTGA GGACCACGCG CAGGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy.
American journal of human genetics99(3)695-703(2016 Sep)
Colin E,Daniel J,Ziegler A,Wakim J,Scrivo A,Haack TB,Khiati S,Denomm? AS,Amati-Bonneau P,Charif M,Procaccio V,Reynier P,Aleck KA,Botto LD,Herper CL,Kaiser CS,Nabbout R,N'Guyen S,Mora-Lorca JA,Assmann B,Christ S,Meitinger T,Strom TM,Prokisch H,,Miranda-Vizuete A,Hoffmann GF,Lenaers G,Bomont P,Liebau E,Bonneau D

A human laterality disorder caused by a homozygous deleterious mutation in MMP21.
Journal of medical genetics52(12)840-7(2015 Dec)
Perles Z,Moon S,Ta-Shma A,Yaacov B,Francescatto L,Edvardson S,Rein AJ,Elpeleg O,Katsanis N

MMP21 is mutated in human heterotaxy and is required for normal left-right asymmetry in vertebrates.
Nature genetics47(11)1260-3(2015 Nov)
Guimier A,Gabriel GC,Bajolle F,Tsang M,Liu H,Noll A,Schwartz M,El Malti R,Smith LD,Klena NT,Jimenez G,Miller NA,Oufadem M,Moreau de Bellaing A,Yagi H,Saunders CJ,Baker CN,Di Filippo S,Peterson KA,Thiffault I,Bole-Feysot C,Cooley LD,Farrow EG,Masson C,Schoen P,Deleuze JF,Nitschk? P,Lyonnet S,de Pontual L,Murray SA,Bonnet D,Kingsmore SF,Amiel J,Bouvagnet P,Lo CW,Gordon CT

The zebrafish embryo: a powerful model system for investigating matrix remodeling.
Zebrafish6(4)347-54(2009 Dec)
Wyatt RA,Keow JY,Harris ND,Hach? CA,Li DH,Crawford BD

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mmp21 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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