MAN2A2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAN2A2
Entrez Gene ID	488745
Full Name	mannosidase alpha class 2A member 2
General protein information	Preferred Names mannosidase alpha class 2A member 2 Names alpha-mannosidase 2x
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_545863.4	XP_545863.3	alpha-mannosidase 2x isoform X2
XM_022416944.1	XP_022272652.1	alpha-mannosidase 2x isoform X1
XM_005618371.1	XP_005618428.1	alpha-mannosidase 2x isoform X1
XM_005618371.2	XP_005618428.1	alpha-mannosidase 2x isoform X1
XM_022416945.1	XP_022272653.1	alpha-mannosidase 2x isoform X2
XM_022416946.1	XP_022272654.1	alpha-mannosidase 2x isoform X3
XM_022416947.1	XP_022272655.1	alpha-mannosidase 2x isoform X4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Man2a2	NP_001100997.1
Oryza sativa (rice)	Os06g0245700	NP_001057277.2
Xenopus tropicalis (tropical clawed frog)	man2a2	NP_001006777.1
Pan troglodytes (chimpanzee)	MAN2A2	XP_001169951.1
Gallus gallus (chicken)	MAN2A2	XP_001233608.2
Danio rerio (zebrafish)	man2a2	XP_005163040.1
Homo sapiens (human)	MAN2A2	NP_006113.2
Bos taurus (cattle)	MAN2A2	NP_001179570.1
Mus musculus (house mouse)	Man2a2	NP_766491.2
Drosophila melanogaster (fruit fly)	alpha-Man-II	NP_524291.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004020	XP_318477.4
Canis lupus familiaris (dog)	MAN2A2	XP_545863.3
Arabidopsis thaliana (thale cress)	GMII	NP_196999.1
Caenorhabditis elegans (roundworm)	aman-2	NP_505995.2

Gene Ontology

The following MAN2A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAN2A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe287230	XM_022416947.1 Latest version!	Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCe287229	XM_022416946.1 Latest version!	Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe04298	XM_022416944.1 Latest version!	Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe04298	XM_005618371.2 Latest version!	Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe04299	XM_022416945.1 Latest version!	Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe04299	XM_545863.4 Latest version!	Canis lupus familiaris mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OCe04298	XM_005618371.1	Canis lupus familiaris mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAN2A2 ( XM_022416947.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272655.1 Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe287230
Clone ID Related Accession (Same CDS sequence)	XM_022416947.1
Accession Version	XM_022416947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	alpha-mannosidase 2x isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG 
TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG 
CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG 
GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT 
GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC 
TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC 
TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA 
ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC 
GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC 
AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT 
GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG 
GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG 
GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG 
GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC 
CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG 
CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT 
GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC 
AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA 
CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG 
ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG 
CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC 
CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT 
GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG 
CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC 
CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC 
ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG 
GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC 
AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG 
GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC 
AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG 
CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GACAGCGCTC AGTGATGCCT 
AATTTGAGCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272655.1
CDS	346..3240
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPALLPYYMANGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTISEELPFDNVDGGVWKQGFDISYSPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWENINAQKKAAVRRLVGNGQLEIVTGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTIPYLLRRANLTSMLIQRVHYAIKKHFASTHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKKTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEILYSLAVAHARRSGLASQYPLSNFALLTEARRTLGLFQHHDAITGTAKEAVVADYGVRLLRSLVNLKQVIINAAHYLVLGDKETYHFDPEAPFLQMDETRLNHDTLPERTVIQLDSSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQISAHWSSATDMVSDVCQVSMPIRLPALGLSVLQLQLGLDGHRTLPSSVRVYLHGRQLAVSRQDAFPLRIIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEQERRVDMEFLIYGTRASKDKSGAYLFLPDREAQPYIPKDPPVLRVTEGPFFSEVVAYYEHIRQVVRLYNLPGVEGLSLDMSSLVDIRDYINKELALRIHTDIDSQGAFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQNRLTLHTAQALGVSSLHDGQRSVMPNLSF

Target ORF information:

RefSeq Version	XM_022416947.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022416947.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG 
TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG 
CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG 
GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT 
GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC 
TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC 
TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA 
ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC 
GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC 
AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT 
GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG 
GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG 
GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG 
GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC 
CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG 
CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT 
GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC 
AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA 
CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG 
ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG 
CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC 
CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT 
GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG 
CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC 
CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC 
ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG 
GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC 
AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG 
GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC 
AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG 
CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GACAGCGCTC AGTGATGCCT 
AATTTGAGCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_022416946.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272654.1 Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe287229
Clone ID Related Accession (Same CDS sequence)	XM_022416946.1
Accession Version	XM_022416946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	alpha-mannosidase 2x isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG 
TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG 
CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG 
GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT 
GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC 
TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC 
TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA 
ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC 
GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC 
AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT 
GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG 
GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG 
GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG 
GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC 
CTGGAAGCCC ACCTGCGGCT TCTGCGCTCC CTTGTCAACC TGAAGCAAGT AATCATCAAT 
GCTGCTCACT ATCTGGTGTT GGGGGACAAG GAGACCTACC ACTTCGACCC TGAGGCACCC 
TTCCTCCAAA TGGATGAAAC CCGCTTAAAT CACGACACAC TGCCAGAGCG CACAGTGATC 
CAGCTAGATT CCTCACCCAG GTATGTGGTG CTCTTCAACC CGCTGGAACA GGAGCGGCTG 
AGTGTGGTGT CCCTGTTGGT CAGCTCGCCC CGGGTGCGGG TCCTTTCAGA GGAGGGCCAG 
CCCCTGGCTG TGCAGATCAG TGCACACTGG AGCTCTGCCA CCGACATGGT CTCTGATGTC 
TGCCAGGTAT CTATGCCCAT CCGCCTGCCG GCCCTGGGCC TCAGCGTGCT ACAGCTGCAG 
CTGGGCCTCG ATGGGCACCG CACCCTGCCC TCCTCTGTGC GTGTCTACCT GCATGGCCGT 
CAGCTGGCCG TCAGCAGGCA GGACGCTTTC CCACTGCGCA TCATTGACTC TGGCACCAGT 
GACTTCGCCC TCAGCAACCG CTACATGCAG GTCTGGTTCT CAGGCCTTAC CGGGCTCCTC 
AAGAGCATCC GAAGGGTGGA CGAGGAGCAG GAGCGGCGGG TGGACATGGA GTTCCTCATC 
TATGGCACCC GCGCGTCCAA AGACAAGAGT GGAGCCTACC TCTTCCTGCC TGACAGGGAG 
GCCCAGCCCT ATATCCCCAA GGACCCCCCC GTGCTGCGTG TCACCGAAGG CCCTTTCTTC 
TCGGAGGTGG TGGCATACTA CGAGCATATT CGCCAGGTGG TCCGGCTATA TAATCTGCCA 
GGGGTGGAGG GGCTGTCTCT GGACATGTCG TCCTTGGTGG ACATCCGGGA TTACATCAAC 
AAGGAGCTGG CCCTGCGCAT CCACACAGAC ATTGACAGCC AGGGTGCCTT CTTCACGGAC 
CTCAATGGCT TTCAGGTGCA GCCCCGGCGC TATCTGAAGA AGCTGCCTCT GCAGGCCAAT 
TTCTACCCCA TGCCAGTCAT GGCCTACATC CAGGATGCGC AGAACCGCCT CACACTGCAC 
ACTGCCCAGG CCCTGGGTGT CTCCAGTCTG CACGATGGCC AGCTGGAGGT GATCCTGGAC 
CGACGGCTCA TGCAAGATGA CAACCGGGGT CTGGGCCAAG GGCTCAAGGA CAATAAAAGA 
ACCTGCAACC ATTTCCGCCT CCTGTTGGAA CGGCGAACCC TGGGCAGTGA GCCTGGCTTT 
TTCTCCAAAC TGGCAGCCAT GTTTAGGGAC TTGATCTTTC ACAGCAGCAG GACCAATAAC 
CTTGAGGTCC AGGATGGCCA CACCAGCAGC TACCCGTCCC TCCTCAGCCA CCTGACCTCC 
ATGTACCTGA ATATCCCAGT GTTTGCCCTG CCTGTGGCCA AGAGGCAGCT CCCCGGCCCT 
GGTCTACGCT CCTTTCATCC CCTGGCCTCC TCCCTGCCCT GTGACTTCCA CCTGCTCAAC 
CTGCGCACCC TGCAGGCCGA GGATGATGCC TTGCCATCAG CAGAGACCGC ACTCATCTTA 
CACCGCAAGG GTTTTGACTG TGGCCTTGAG GCCAAGAACT TGGGCTTCAA CTGCAGCACA 
AGCCAAGGCA AGGCAGCCCT GGGGAGCCTC TTCCATGGCC TGGATGTAGG TTTCCTGCAG 
CCGACCTCAC TGACATTACT GTACCCACTG GCCGCACCCT CCAACAGCAC TGATGTCTAC 
CTGGAGCCCA TGGAGATTGC CACCTTTCGC CTCCGCTTGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272654.1
CDS	346..3690
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPALLPYYMANGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTISEELPFDNVDGGVWKQGFDISYSPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWENINAQKKAAVRRLVGNGQLEIVTGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTIPYLLRRANLTSMLIQRVHYAIKKHFASTHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKKTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRLLRSLVNLKQVIINAAHYLVLGDKETYHFDPEAPFLQMDETRLNHDTLPERTVIQLDSSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQISAHWSSATDMVSDVCQVSMPIRLPALGLSVLQLQLGLDGHRTLPSSVRVYLHGRQLAVSRQDAFPLRIIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEQERRVDMEFLIYGTRASKDKSGAYLFLPDREAQPYIPKDPPVLRVTEGPFFSEVVAYYEHIRQVVRLYNLPGVEGLSLDMSSLVDIRDYINKELALRIHTDIDSQGAFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQNRLTLHTAQALGVSSLHDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGSEPGFFSKLAAMFRDLIFHSSRTNNLEVQDGHTSSYPSLLSHLTSMYLNIPVFALPVAKRQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEDDALPSAETALILHRKGFDCGLEAKNLGFNCSTSQGKAALGSLFHGLDVGFLQPTSLTLLYPLAAPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_022416946.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022416946.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG 
TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG 
CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG 
GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT 
GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC 
TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC 
TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA 
ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC 
GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC 
AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT 
GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG 
GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG 
GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG 
GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC 
CTGGAAGCCC ACCTGCGGCT TCTGCGCTCC CTTGTCAACC TGAAGCAAGT AATCATCAAT 
GCTGCTCACT ATCTGGTGTT GGGGGACAAG GAGACCTACC ACTTCGACCC TGAGGCACCC 
TTCCTCCAAA TGGATGAAAC CCGCTTAAAT CACGACACAC TGCCAGAGCG CACAGTGATC 
CAGCTAGATT CCTCACCCAG GTATGTGGTG CTCTTCAACC CGCTGGAACA GGAGCGGCTG 
AGTGTGGTGT CCCTGTTGGT CAGCTCGCCC CGGGTGCGGG TCCTTTCAGA GGAGGGCCAG 
CCCCTGGCTG TGCAGATCAG TGCACACTGG AGCTCTGCCA CCGACATGGT CTCTGATGTC 
TGCCAGGTAT CTATGCCCAT CCGCCTGCCG GCCCTGGGCC TCAGCGTGCT ACAGCTGCAG 
CTGGGCCTCG ATGGGCACCG CACCCTGCCC TCCTCTGTGC GTGTCTACCT GCATGGCCGT 
CAGCTGGCCG TCAGCAGGCA GGACGCTTTC CCACTGCGCA TCATTGACTC TGGCACCAGT 
GACTTCGCCC TCAGCAACCG CTACATGCAG GTCTGGTTCT CAGGCCTTAC CGGGCTCCTC 
AAGAGCATCC GAAGGGTGGA CGAGGAGCAG GAGCGGCGGG TGGACATGGA GTTCCTCATC 
TATGGCACCC GCGCGTCCAA AGACAAGAGT GGAGCCTACC TCTTCCTGCC TGACAGGGAG 
GCCCAGCCCT ATATCCCCAA GGACCCCCCC GTGCTGCGTG TCACCGAAGG CCCTTTCTTC 
TCGGAGGTGG TGGCATACTA CGAGCATATT CGCCAGGTGG TCCGGCTATA TAATCTGCCA 
GGGGTGGAGG GGCTGTCTCT GGACATGTCG TCCTTGGTGG ACATCCGGGA TTACATCAAC 
AAGGAGCTGG CCCTGCGCAT CCACACAGAC ATTGACAGCC AGGGTGCCTT CTTCACGGAC 
CTCAATGGCT TTCAGGTGCA GCCCCGGCGC TATCTGAAGA AGCTGCCTCT GCAGGCCAAT 
TTCTACCCCA TGCCAGTCAT GGCCTACATC CAGGATGCGC AGAACCGCCT CACACTGCAC 
ACTGCCCAGG CCCTGGGTGT CTCCAGTCTG CACGATGGCC AGCTGGAGGT GATCCTGGAC 
CGACGGCTCA TGCAAGATGA CAACCGGGGT CTGGGCCAAG GGCTCAAGGA CAATAAAAGA 
ACCTGCAACC ATTTCCGCCT CCTGTTGGAA CGGCGAACCC TGGGCAGTGA GCCTGGCTTT 
TTCTCCAAAC TGGCAGCCAT GTTTAGGGAC TTGATCTTTC ACAGCAGCAG GACCAATAAC 
CTTGAGGTCC AGGATGGCCA CACCAGCAGC TACCCGTCCC TCCTCAGCCA CCTGACCTCC 
ATGTACCTGA ATATCCCAGT GTTTGCCCTG CCTGTGGCCA AGAGGCAGCT CCCCGGCCCT 
GGTCTACGCT CCTTTCATCC CCTGGCCTCC TCCCTGCCCT GTGACTTCCA CCTGCTCAAC 
CTGCGCACCC TGCAGGCCGA GGATGATGCC TTGCCATCAG CAGAGACCGC ACTCATCTTA 
CACCGCAAGG GTTTTGACTG TGGCCTTGAG GCCAAGAACT TGGGCTTCAA CTGCAGCACA 
AGCCAAGGCA AGGCAGCCCT GGGGAGCCTC TTCCATGGCC TGGATGTAGG TTTCCTGCAG 
CCGACCTCAC TGACATTACT GTACCCACTG GCCGCACCCT CCAACAGCAC TGATGTCTAC 
CTGGAGCCCA TGGAGATTGC CACCTTTCGC CTCCGCTTGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_022416944.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272652.1 Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.

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CloneID	OCe04298
Clone ID Related Accession (Same CDS sequence)	XM_022416944.1 , XM_005618371.1 , XM_005618371.2
Accession Version	XM_022416944.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG 
TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG 
CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG 
GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT 
GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC 
TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC 
TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA 
ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC 
GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC 
AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT 
GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG 
GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG 
GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG 
GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC 
CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG 
CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT 
GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC 
AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA 
CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG 
ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG 
CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC 
CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT 
GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG 
CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC 
CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC 
ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG 
GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC 
AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG 
GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC 
AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG 
CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG 
GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA 
AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GACTTGATCT TTCACAGCAG CAGGACCAAT 
AACCTTGAGG TCCAGGATGG CCACACCAGC AGCTACCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAATATCCC AGTGTTTGCC CTGCCTGTGG CCAAGAGGCA GCTCCCCGGC 
CCTGGTCTAC GCTCCTTTCA TCCCCTGGCC TCCTCCCTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGCA CCCTGCAGGC CGAGGATGAT GCCTTGCCAT CAGCAGAGAC CGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCAGC 
ACAAGCCAAG GCAAGGCAGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT AGGTTTCCTG 
CAGCCGACCT CACTGACATT ACTGTACCCA CTGGCCGCAC CCTCCAACAG CACTGATGTC 
TACCTGGAGC CCATGGAGAT TGCCACCTTT CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272652.1
CDS	1..3528
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPALLPYYMANGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTISEELPFDNVDGGVWKQGFDISYSPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWENINAQKKAAVRRLVGNGQLEIVTGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTIPYLLRRANLTSMLIQRVHYAIKKHFASTHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKKTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEILYSLAVAHARRSGLASQYPLSNFALLTEARRTLGLFQHHDAITGTAKEAVVADYGVRLLRSLVNLKQVIINAAHYLVLGDKETYHFDPEAPFLQMDETRLNHDTLPERTVIQLDSSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQISAHWSSATDMVSDVCQVSMPIRLPALGLSVLQLQLGLDGHRTLPSSVRVYLHGRQLAVSRQDAFPLRIIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEQERRVDMEFLIYGTRASKDKSGAYLFLPDREAQPYIPKDPPVLRVTEGPFFSEVVAYYEHIRQVVRLYNLPGVEGLSLDMSSLVDIRDYINKELALRIHTDIDSQGAFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQNRLTLHTAQALGVSSLHDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGSEPGFFSKLAAMFRDLIFHSSRTNNLEVQDGHTSSYPSLLSHLTSMYLNIPVFALPVAKRQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEDDALPSAETALILHRKGFDCGLEAKNLGFNCSTSQGKAALGSLFHGLDVGFLQPTSLTLLYPLAAPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_022416944.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022416944.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG 
TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG 
CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG 
GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT 
GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC 
TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC 
TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA 
ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC 
GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC 
AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT 
GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG 
GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG 
GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG 
GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC 
CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG 
CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT 
GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC 
AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA 
CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG 
ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG 
CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC 
CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT 
GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG 
CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC 
CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC 
ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG 
GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC 
AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG 
GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC 
AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG 
CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG 
GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA 
AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GACTTGATCT TTCACAGCAG CAGGACCAAT 
AACCTTGAGG TCCAGGATGG CCACACCAGC AGCTACCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAATATCCC AGTGTTTGCC CTGCCTGTGG CCAAGAGGCA GCTCCCCGGC 
CCTGGTCTAC GCTCCTTTCA TCCCCTGGCC TCCTCCCTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGCA CCCTGCAGGC CGAGGATGAT GCCTTGCCAT CAGCAGAGAC CGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCAGC 
ACAAGCCAAG GCAAGGCAGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT AGGTTTCCTG 
CAGCCGACCT CACTGACATT ACTGTACCCA CTGGCCGCAC CCTCCAACAG CACTGATGTC 
TACCTGGAGC CCATGGAGAT TGCCACCTTT CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_005618371.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618428.1 Canis lupus familiaris mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X1, mRNA.

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CloneID	OCe04298
Clone ID Related Accession (Same CDS sequence)	XM_022416944.1 , XM_005618371.1 , XM_005618371.2
Accession Version	XM_005618371.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	alpha-mannosidase 2x isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG 
TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG 
CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG 
GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT 
GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC 
TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC 
TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA 
ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC 
GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC 
AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT 
GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG 
GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG 
GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG 
GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC 
CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG 
CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT 
GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC 
AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA 
CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG 
ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG 
CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC 
CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT 
GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG 
CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC 
CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC 
ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG 
GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC 
AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG 
GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC 
AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG 
CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG 
GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA 
AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GACTTGATCT TTCACAGCAG CAGGACCAAT 
AACCTTGAGG TCCAGGATGG CCACACCAGC AGCTACCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAATATCCC AGTGTTTGCC CTGCCTGTGG CCAAGAGGCA GCTCCCCGGC 
CCTGGTCTAC GCTCCTTTCA TCCCCTGGCC TCCTCCCTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGCA CCCTGCAGGC CGAGGATGAT GCCTTGCCAT CAGCAGAGAC CGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCAGC 
ACAAGCCAAG GCAAGGCAGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT AGGTTTCCTG 
CAGCCGACCT CACTGACATT ACTGTACCCA CTGGCCGCAC CCTCCAACAG CACTGATGTC 
TACCTGGAGC CCATGGAGAT TGCCACCTTT CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618428.1
CDS	346..3873
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPALLPYYMANGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTISEELPFDNVDGGVWKQGFDISYSPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWENINAQKKAAVRRLVGNGQLEIVTGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTIPYLLRRANLTSMLIQRVHYAIKKHFASTHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKKTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEILYSLAVAHARRSGLASQYPLSNFALLTEARRTLGLFQHHDAITGTAKEAVVADYGVRLLRSLVNLKQVIINAAHYLVLGDKETYHFDPEAPFLQMDETRLNHDTLPERTVIQLDSSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQISAHWSSATDMVSDVCQVSMPIRLPALGLSVLQLQLGLDGHRTLPSSVRVYLHGRQLAVSRQDAFPLRIIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEQERRVDMEFLIYGTRASKDKSGAYLFLPDREAQPYIPKDPPVLRVTEGPFFSEVVAYYEHIRQVVRLYNLPGVEGLSLDMSSLVDIRDYINKELALRIHTDIDSQGAFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQNRLTLHTAQALGVSSLHDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGSEPGFFSKLAAMFRDLIFHSSRTNNLEVQDGHTSSYPSLLSHLTSMYLNIPVFALPVAKRQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEDDALPSAETALILHRKGFDCGLEAKNLGFNCSTSQGKAALGSLFHGLDVGFLQPTSLTLLYPLAAPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_005618371.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618371.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG 
TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG 
CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG 
GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT 
GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC 
TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC 
TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA 
ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC 
GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC 
AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT 
GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG 
GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG 
GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG 
GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC 
CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG 
CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT 
GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC 
AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA 
CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG 
ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG 
CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC 
CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT 
GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG 
CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC 
CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC 
ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG 
GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC 
AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG 
GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC 
AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG 
CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG 
GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA 
AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GACTTGATCT TTCACAGCAG CAGGACCAAT 
AACCTTGAGG TCCAGGATGG CCACACCAGC AGCTACCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAATATCCC AGTGTTTGCC CTGCCTGTGG CCAAGAGGCA GCTCCCCGGC 
CCTGGTCTAC GCTCCTTTCA TCCCCTGGCC TCCTCCCTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGCA CCCTGCAGGC CGAGGATGAT GCCTTGCCAT CAGCAGAGAC CGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCAGC 
ACAAGCCAAG GCAAGGCAGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT AGGTTTCCTG 
CAGCCGACCT CACTGACATT ACTGTACCCA CTGGCCGCAC CCTCCAACAG CACTGATGTC 
TACCTGGAGC CCATGGAGAT TGCCACCTTT CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_005618371.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618428.1 Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.

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CloneID	OCe04298
Clone ID Related Accession (Same CDS sequence)	XM_022416944.1 , XM_005618371.1 , XM_005618371.2
Accession Version	XM_005618371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618371.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG 
TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG 
CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG 
GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT 
GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC 
TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC 
TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA 
ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC 
GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC 
AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT 
GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG 
GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG 
GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG 
GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC 
CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG 
CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT 
GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC 
AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA 
CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG 
ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG 
CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC 
CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT 
GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG 
CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC 
CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC 
ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG 
GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC 
AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG 
GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC 
AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG 
CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG 
GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA 
AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GACTTGATCT TTCACAGCAG CAGGACCAAT 
AACCTTGAGG TCCAGGATGG CCACACCAGC AGCTACCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAATATCCC AGTGTTTGCC CTGCCTGTGG CCAAGAGGCA GCTCCCCGGC 
CCTGGTCTAC GCTCCTTTCA TCCCCTGGCC TCCTCCCTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGCA CCCTGCAGGC CGAGGATGAT GCCTTGCCAT CAGCAGAGAC CGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCAGC 
ACAAGCCAAG GCAAGGCAGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT AGGTTTCCTG 
CAGCCGACCT CACTGACATT ACTGTACCCA CTGGCCGCAC CCTCCAACAG CACTGATGTC 
TACCTGGAGC CCATGGAGAT TGCCACCTTT CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618428.1
CDS	346..3873
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPALLPYYMANGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTISEELPFDNVDGGVWKQGFDISYSPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWENINAQKKAAVRRLVGNGQLEIVTGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTIPYLLRRANLTSMLIQRVHYAIKKHFASTHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKKTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEILYSLAVAHARRSGLASQYPLSNFALLTEARRTLGLFQHHDAITGTAKEAVVADYGVRLLRSLVNLKQVIINAAHYLVLGDKETYHFDPEAPFLQMDETRLNHDTLPERTVIQLDSSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQISAHWSSATDMVSDVCQVSMPIRLPALGLSVLQLQLGLDGHRTLPSSVRVYLHGRQLAVSRQDAFPLRIIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEQERRVDMEFLIYGTRASKDKSGAYLFLPDREAQPYIPKDPPVLRVTEGPFFSEVVAYYEHIRQVVRLYNLPGVEGLSLDMSSLVDIRDYINKELALRIHTDIDSQGAFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQNRLTLHTAQALGVSSLHDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGSEPGFFSKLAAMFRDLIFHSSRTNNLEVQDGHTSSYPSLLSHLTSMYLNIPVFALPVAKRQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEDDALPSAETALILHRKGFDCGLEAKNLGFNCSTSQGKAALGSLFHGLDVGFLQPTSLTLLYPLAAPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_005618371.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618371.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG 
TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG 
CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG 
GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT 
GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC 
TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC 
TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA 
ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC 
GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC 
AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT 
GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG 
GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG 
GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG 
GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC 
CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG 
CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT 
GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC 
AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA 
CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG 
ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG 
CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC 
CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT 
GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG 
CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC 
CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC 
ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG 
GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC 
AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG 
GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC 
AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG 
CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG 
GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA 
AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GACTTGATCT TTCACAGCAG CAGGACCAAT 
AACCTTGAGG TCCAGGATGG CCACACCAGC AGCTACCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAATATCCC AGTGTTTGCC CTGCCTGTGG CCAAGAGGCA GCTCCCCGGC 
CCTGGTCTAC GCTCCTTTCA TCCCCTGGCC TCCTCCCTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGCA CCCTGCAGGC CGAGGATGAT GCCTTGCCAT CAGCAGAGAC CGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCAGC 
ACAAGCCAAG GCAAGGCAGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT AGGTTTCCTG 
CAGCCGACCT CACTGACATT ACTGTACCCA CTGGCCGCAC CCTCCAACAG CACTGATGTC 
TACCTGGAGC CCATGGAGAT TGCCACCTTT CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_545863.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_545863.3 Canis lupus familiaris mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X2, mRNA.

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CloneID	OCe04299
Clone ID Related Accession (Same CDS sequence)	XM_545863.4 , XM_022416945.1
Accession Version	XM_545863.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	alpha-mannosidase 2x isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_545863.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG 
TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG 
CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG 
GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT 
GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC 
TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC 
TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA 
ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC 
GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC 
AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT 
GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG 
GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG 
GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG 
GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC 
CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG 
CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT 
GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC 
AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA 
CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG 
ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG 
CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC 
CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT 
GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG 
CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC 
CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC 
ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG 
GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC 
AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG 
GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC 
AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG 
CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG 
GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA 
AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGGTCCAG 
GATGGCCACA CCAGCAGCTA CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAT 
ATCCCAGTGT TTGCCCTGCC TGTGGCCAAG AGGCAGCTCC CCGGCCCTGG TCTACGCTCC 
TTTCATCCCC TGGCCTCCTC CCTGCCCTGT GACTTCCACC TGCTCAACCT GCGCACCCTG 
CAGGCCGAGG ATGATGCCTT GCCATCAGCA GAGACCGCAC TCATCTTACA CCGCAAGGGT 
TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGCTTCAACT GCAGCACAAG CCAAGGCAAG 
GCAGCCCTGG GGAGCCTCTT CCATGGCCTG GATGTAGGTT TCCTGCAGCC GACCTCACTG 
ACATTACTGT ACCCACTGGC CGCACCCTCC AACAGCACTG ATGTCTACCT GGAGCCCATG 
GAGATTGCCA CCTTTCGCCT CCGCTTGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_545863.3
CDS	346..3798
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPALLPYYMANGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTISEELPFDNVDGGVWKQGFDISYSPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWENINAQKKAAVRRLVGNGQLEIVTGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTIPYLLRRANLTSMLIQRVHYAIKKHFASTHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKKTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEILYSLAVAHARRSGLASQYPLSNFALLTEARRTLGLFQHHDAITGTAKEAVVADYGVRLLRSLVNLKQVIINAAHYLVLGDKETYHFDPEAPFLQMDETRLNHDTLPERTVIQLDSSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQISAHWSSATDMVSDVCQVSMPIRLPALGLSVLQLQLGLDGHRTLPSSVRVYLHGRQLAVSRQDAFPLRIIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEQERRVDMEFLIYGTRASKDKSGAYLFLPDREAQPYIPKDPPVLRVTEGPFFSEVVAYYEHIRQVVRLYNLPGVEGLSLDMSSLVDIRDYINKELALRIHTDIDSQGAFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQNRLTLHTAQALGVSSLHDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGSEVQDGHTSSYPSLLSHLTSMYLNIPVFALPVAKRQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEDDALPSAETALILHRKGFDCGLEAKNLGFNCSTSQGKAALGSLFHGLDVGFLQPTSLTLLYPLAAPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_545863.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_545863.4

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG 
TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG 
CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG 
GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT 
GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC 
TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC 
TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA 
ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC 
GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC 
AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT 
GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG 
GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG 
GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG 
GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC 
CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG 
CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT 
GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC 
AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA 
CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG 
ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG 
CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC 
CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT 
GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG 
CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC 
CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC 
ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG 
GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC 
AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG 
GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC 
AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG 
CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG 
GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA 
AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGGTCCAG 
GATGGCCACA CCAGCAGCTA CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAT 
ATCCCAGTGT TTGCCCTGCC TGTGGCCAAG AGGCAGCTCC CCGGCCCTGG TCTACGCTCC 
TTTCATCCCC TGGCCTCCTC CCTGCCCTGT GACTTCCACC TGCTCAACCT GCGCACCCTG 
CAGGCCGAGG ATGATGCCTT GCCATCAGCA GAGACCGCAC TCATCTTACA CCGCAAGGGT 
TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGCTTCAACT GCAGCACAAG CCAAGGCAAG 
GCAGCCCTGG GGAGCCTCTT CCATGGCCTG GATGTAGGTT TCCTGCAGCC GACCTCACTG 
ACATTACTGT ACCCACTGGC CGCACCCTCC AACAGCACTG ATGTCTACCT GGAGCCCATG 
GAGATTGCCA CCTTTCGCCT CCGCTTGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_022416945.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272653.1 Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe04299
Clone ID Related Accession (Same CDS sequence)	XM_545863.4 , XM_022416945.1
Accession Version	XM_022416945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	alpha-mannosidase 2x isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG 
TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG 
CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG 
GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT 
GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC 
TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC 
TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA 
ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC 
GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC 
AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT 
GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG 
GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG 
GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG 
GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC 
CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG 
CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT 
GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC 
AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA 
CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG 
ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG 
CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC 
CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT 
GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG 
CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC 
CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC 
ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG 
GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC 
AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG 
GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC 
AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG 
CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG 
GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA 
AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGGTCCAG 
GATGGCCACA CCAGCAGCTA CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAT 
ATCCCAGTGT TTGCCCTGCC TGTGGCCAAG AGGCAGCTCC CCGGCCCTGG TCTACGCTCC 
TTTCATCCCC TGGCCTCCTC CCTGCCCTGT GACTTCCACC TGCTCAACCT GCGCACCCTG 
CAGGCCGAGG ATGATGCCTT GCCATCAGCA GAGACCGCAC TCATCTTACA CCGCAAGGGT 
TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGCTTCAACT GCAGCACAAG CCAAGGCAAG 
GCAGCCCTGG GGAGCCTCTT CCATGGCCTG GATGTAGGTT TCCTGCAGCC GACCTCACTG 
ACATTACTGT ACCCACTGGC CGCACCCTCC AACAGCACTG ATGTCTACCT GGAGCCCATG 
GAGATTGCCA CCTTTCGCCT CCGCTTGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272653.1
CDS	1..3453
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPALLPYYMANGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTISEELPFDNVDGGVWKQGFDISYSPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWENINAQKKAAVRRLVGNGQLEIVTGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTIPYLLRRANLTSMLIQRVHYAIKKHFASTHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKKTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEILYSLAVAHARRSGLASQYPLSNFALLTEARRTLGLFQHHDAITGTAKEAVVADYGVRLLRSLVNLKQVIINAAHYLVLGDKETYHFDPEAPFLQMDETRLNHDTLPERTVIQLDSSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQISAHWSSATDMVSDVCQVSMPIRLPALGLSVLQLQLGLDGHRTLPSSVRVYLHGRQLAVSRQDAFPLRIIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEQERRVDMEFLIYGTRASKDKSGAYLFLPDREAQPYIPKDPPVLRVTEGPFFSEVVAYYEHIRQVVRLYNLPGVEGLSLDMSSLVDIRDYINKELALRIHTDIDSQGAFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQNRLTLHTAQALGVSSLHDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGSEVQDGHTSSYPSLLSHLTSMYLNIPVFALPVAKRQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEDDALPSAETALILHRKGFDCGLEAKNLGFNCSTSQGKAALGSLFHGLDVGFLQPTSLTLLYPLAAPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_022416945.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022416945.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG 
TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG 
CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG 
GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT 
GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC 
TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC 
TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA 
ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC 
GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC 
AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT 
GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG 
GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG 
GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG 
GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC 
CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG 
CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT 
GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC 
AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA 
CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG 
ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG 
CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC 
CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT 
GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG 
CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC 
CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC 
ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG 
GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC 
AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG 
GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC 
AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG 
CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG 
GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA 
AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGGTCCAG 
GATGGCCACA CCAGCAGCTA CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAT 
ATCCCAGTGT TTGCCCTGCC TGTGGCCAAG AGGCAGCTCC CCGGCCCTGG TCTACGCTCC 
TTTCATCCCC TGGCCTCCTC CCTGCCCTGT GACTTCCACC TGCTCAACCT GCGCACCCTG 
CAGGCCGAGG ATGATGCCTT GCCATCAGCA GAGACCGCAC TCATCTTACA CCGCAAGGGT 
TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGCTTCAACT GCAGCACAAG CCAAGGCAAG 
GCAGCCCTGG GGAGCCTCTT CCATGGCCTG GATGTAGGTT TCCTGCAGCC GACCTCACTG 
ACATTACTGT ACCCACTGGC CGCACCCTCC AACAGCACTG ATGTCTACCT GGAGCCCATG 
GAGATTGCCA CCTTTCGCCT CCGCTTGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MAN2A2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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