xrn-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	xrn-2
Entrez Gene ID	175071
Full Name	5'-3' exoribonuclease 2 homolog
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_064557.5	NP_496958.2	5'-3' exoribonuclease 2 homolog
NM_064557.6	NP_496958.2	5'-3' exoribonuclease 2 homolog

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AGR303W	NP_986969.2
Pan troglodytes (chimpanzee)	XRN2	XP_514546.2
Canis lupus familiaris (dog)	XRN2	XP_534324.2
Rattus norvegicus (Norway rat)	Xrn2	NP_001102066.1
Drosophila melanogaster (fruit fly)	Rat1	NP_001260166.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004090	XP_309641.5
Caenorhabditis elegans (roundworm)	xrn-2	NP_496958.2
Saccharomyces cerevisiae (baker's yeast)	RAT1	NP_014691.1
Xenopus tropicalis (tropical clawed frog)	xrn2	XP_002939479.1
Homo sapiens (human)	XRN2	NP_036387.2
Bos taurus (cattle)	XRN2	NP_001179401.1
Mus musculus (house mouse)	Xrn2	NP_036047.2
Schizosaccharomyces pombe (fission yeast)	dhp1	NP_594155.1
Neurospora crassa	NCU01643	XP_956265.1
	XRN2	XP_001094734.2
Gallus gallus (chicken)	XRN2	NP_001026204.1
Danio rerio (zebrafish)	xrn2	NP_001001944.2
Magnaporthe oryzae (rice blast fungus)	MGG_11426	XP_003717803.1
Kluyveromyces lactis	KLLA0F07469g	XP_455419.1
Arabidopsis thaliana (thale cress)	XRN3	NP_565114.1
Oryza sativa (rice)	Os01g0872700	NP_001044947.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

De-repression appears to be triggered such that more robust XRN2 perturbation, by elimination of both PAXT-1 and BPNT1, is less detrimental to worm viability than absence of PAXT-1 alone. Indeed, we find that two distinct XRN2 repression mechanisms are alleviated at different thresholds of XRN2 inactivation. Like more than 15% of C. elegans genes, xrn-2 occurs in an operon, and we identify additional
Title: XRN2 Autoregulation and Control of Polycistronic Gene Expresssion in Caenorhabditis elegans.

In Caenorhabditis elegans, XRN-2 functions with PAXT-1, which shares a putative XRN-2-binding domain (XTBD) with otherwise unrelated mammalian proteins. The study characterizes the structure and function of an XTBD-XRN2 complex.
Title: Structural basis and function of XRN2 binding by XTB domains.

XRN2 has unanticipated miRNA specificity in vivo, and its diverse developmental functions may relate to distinct substrates.
Title: Engineering of a conditional allele reveals multiple roles of XRN2 in Caenorhabditis elegans development and substrate specificity in microRNA turnover.

degradation of mature miRNAs in the nematode Caenorhabditis elegans, mediated by the 5'-->3' exoribonuclease XRN-2, affects functional miRNA homeostasis in vivo
Title: Active turnover modulates mature microRNA activity in Caenorhabditis elegans.

The following xrn-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the xrn-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa337786	NM_064557.6 Latest version!	Caenorhabditis elegans 5'-3' exoribonuclease 2 homolog (xrn-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa337786	NM_064557.5	Caenorhabditis elegans 5'-3' exoribonuclease 2 homolog (xrn-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

xrn-2 ( NM_064557.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496958.2 Caenorhabditis elegans 5'-3' exoribonuclease 2 homolog (xrn-2), mRNA.

Price & Availability↑

CloneID	OCa337786
Clone ID Related Accession (Same CDS sequence)	NM_064557.5 , NM_064557.6
Accession Version	NM_064557.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	5'-3' exoribonuclease 2 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_064557.5.

ATGGGAGTTC CCGCATTCTT CAGATGGTTG ACTAAAAAGT ATCCGGCAAC CGTGGTGAAT 
GCCAATGAAG ATCGTCAGCG AGACCAGGAT GGCAACCGGG TCCCCGTAGA TTGTACACAG 
CCCAATCCGA ATTTCCAGGA GTTTGATAAT CTATACCTGG ATATGAACGG TATTATCCAT 
CCGTGCACCC ATCCAGAAGA CAGGCCGGCT CCGAAGAACG AAGATGAGAT GTTCGCGCTC 
ATTTTCGAGT ACATCGATCG CATTTATTCG ATTGTCAGAC CGCGAAGGCT TCTCTATATG 
GCGATCGATG GAGTTGCTCC GCGAGCCAAG ATGAACCAGC AGCGAAGTCG TCGATTCCGG 
GCGAGTAAAG AGATGGCTGA AAAGGAGGCG AGCATCGAAG AGCAAAGAAA TCGATTGATG 
GCCGAAGGCA TCGCTGTGCC ACCGAAAAAG AAAGAAGAAG CTCATTTCGA TAGTAACTGT 
ATTACTCCTG GAACACCATT TATGGCTCGA CTCGCCGATG CTCTTCGATA TTATATTCAT 
GATCGGGTCA CTAATGACGC GTCATGGGCT AATATTGAGA TCATCCTCTC CGACGCGAAT 
GTTCCCGGCG AAGGCGAGCA CAAGATCATG GACTACGTCC GAAAGCAACG TGGCAACCCG 
GCTCACGACC CGAACACCGT CCACTGCCTC TGCGGAGCCG ACGCCGACCT TATTATGCTC 
GGAATCGCCA CCCACGAGGC CAACTTCAAT ATTATCCGAG AGGAGTTCGT GCCGAATCAG 
CCGCGGGCTT GTGATCTATG CGGTCAGTAC GGTCATGAGC TGAAGGAGTG TAGAGGCGCC 
GAAAATGAGA CCGATTTGGG CGATGACTAC TGTAAGCCGG AGCAGCGAGA GAAGAATTTC 
ATTTTTCTAC GGATCCCGGT GCTCAGGGAG TACTTGGAAA AGGAGCTCTC AATGCCGAAT 
CTTCCATTTA AATTCGACGT CGAGCGGGCT CTGGATGATT GGGTTTTCCT GTGCTTCTTC 
GTCGGAAACG ACTTCCTTCC ACATTTACCA TCGCTCGAGA TCCGAGAAGG GGCAATTGAC 
AGACTGATTA AGCTGTATAA GGAGATGGTC TACCAGATGA AAGGTTATCT GACAAAGGAC 
GGGATCCCGG AGCTCGATCG GGTCGAGATG ATTATGAAGG GGCTCGGACG CGTCGAAGAT 
GAGATTTTCA AGCGAAGACA GCAGGATGAG GAGCGATTCC AGGAAAATCA GCGAAACAAG 
AAAGCGAGAA TGCAGATGTA CGGAGGAGGA GGAAGAGGAG GTCGTGGTCG TGGTAGAGGA 
AGAGGTCAAC AACCGGCTTT TGTGCCGACG CACGGGATTC TGGCTCCGAT GGCGGCTCCG 
ATGCATCATT CGGGAGAGTC GACGAGGCAG ATGGCCTCGG AAGCCAGGCA GACGGCCATG 
AAGTTCACCA ACGATGCCAA TGAAACGGCT GCGGCGAATT TGAAGGCTTT GCTCAATGTG 
AAAGGTGAAG AATCGCCAGC AGACATTGCG TCAAGAAAAC GAAAAGCCGA GCAGCCACTG 
ATAAAGCCTG AAGAAGAGGA AGATGAAGGA CCAAAAGATG ACATTCGTCT CTACGAATCA 
GGCTGGAAGG ATCGATATTA CCGAGCGAAA TTCGACGTCG GATCCGACGA TATCGAGTTT 
CGGCACCGTG TCGCGTGGGC GTATGTGGAG GGTCTCTGCT GGGTACTACG GTACTATTAT 
CAAGGATGTG CCAGCTGGGA TTGGTATTTC CCGTATCATT ATGCACCGTT TGCCAGCGAT 
TTCGAGACTG TCGGAGAGTT TCAGCCCGAT TTTACGAGGC CTACCAAGCC GTTCAATCCG 
TTGGAGCAGC TGATGAGTGT TTTTCCGGCT GCGTCGAAAC AACATCTCCC AGTGGAATGG 
CAAAAGCTGA TGATTCAAGA CGATTCTCCA ATTATCGATC TCTATCCAGC AGATTTTCGC 
ATCGATCTCA ACGGAAAGAA GTACGCGTGG CAAGGAGTTG CTCTTCTGCC ATTTGTCGAT 
GAAACTCGCC TTCTCGCCAC CCTACAATCC GTCTATCCAA CACTGACCGC CGAGGAAAAG 
CAACGAAATA CACGTGGACC GAATCGAATT TTCATTGGAC GGAATCACAA GTCGTTCGAG 
TTCTTTCAAC AAGTCGCCGA GTCGAAATCC GATGATTTGG TTCCACTGGA TCCGACGCTT 
TTGAACGGAG TTAGCGGAAA AATCGCCTAT GATTCTACGG CAACAGCGCC CGGATTGCCG 
TTCGTATCGC CTGTCAATCA CGATGAATGC CAGGATTTGC CGACCAACTG CGGAATATGT 
GTGCTCTACG AAGATCCCGA GTACCCACAA GACTACATAT TCCCGGCACT CCGTCTCGAC 
GGAGCCAAGG AGCCCGAGAA AACGCTGAAA CCCGACGACT GGAATGATAG ACGAGACGGG 
CGGTATCAGC CGCAAGTGGG CTTCAATCGG AACGCGCCAC GTGGAAGCCT GGATCAATCG 
GGCCATCGAC AGGTGCATCA CTATGTGAGA GGTGGTGGTG GCGGCGGCGG CGGATACCGA 
GGAAATAGCT ATGATGATCG GAGAGGCGGT GGTGGTGGTG GTGGTGGATA CAACGATCGT 
CAAGATTTCG GCCGAAACTA CGGAGGAAGA GACGGTGGAG GTCCCCAGAG ATACCATGAT 
CAGCAGCAGC AGCGTCAAGG AGGCTATCAA GGAGGCGGAT ATGGAGGCGG TTATGGCGGC 
GGCGGCGGAG GTGGTGGTGG TGGTGGTGGA GGATCCTACC ACCAGCCGTA CAATCAGGAT 
CAGAGACGTG GAGGACGAGG TGGTGGTGGT GGGCCACCGG GATATCAGAG ACCACCGTAT 
CGAGGTGGCG GAGGTGGAGG CTATCACGGA AATTCATCAT GGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496958.2
CDS	20..2947
Translation	MGVPAFFRWLTKKYPATVVNANEDRQRDQDGNRVPVDCTQPNPNFQEFDNLYLDMNGIIHPCTHPEDRPAPKNEDEMFALIFEYIDRIYSIVRPRRLLYMAIDGVAPRAKMNQQRSRRFRASKEMAEKEASIEEQRNRLMAEGIAVPPKKKEEAHFDSNCITPGTPFMARLADALRYYIHDRVTNDASWANIEIILSDANVPGEGEHKIMDYVRKQRGNPAHDPNTVHCLCGADADLIMLGIATHEANFNIIREEFVPNQPRACDLCGQYGHELKECRGAENETDLGDDYCKPEQREKNFIFLRIPVLREYLEKELSMPNLPFKFDVERALDDWVFLCFFVGNDFLPHLPSLEIREGAIDRLIKLYKEMVYQMKGYLTKDGIPELDRVEMIMKGLGRVEDEIFKRRQQDEERFQENQRNKKARMQMYGGGGRGGRGRGRGRGQQPAFVPTHGILAPMAAPMHHSGESTRQMASEARQTAMKFTNDANETAAANLKALLNVKGEESPADIASRKRKAEQPLIKPEEEEDEGPKDDIRLYESGWKDRYYRAKFDVGSDDIEFRHRVAWAYVEGLCWVLRYYYQGCASWDWYFPYHYAPFASDFETVGEFQPDFTRPTKPFNPLEQLMSVFPAASKQHLPVEWQKLMIQDDSPIIDLYPADFRIDLNGKKYAWQGVALLPFVDETRLLATLQSVYPTLTAEEKQRNTRGPNRIFIGRNHKSFEFFQQVAESKSDDLVPLDPTLLNGVSGKIAYDSTATAPGLPFVSPVNHDECQDLPTNCGICVLYEDPEYPQDYIFPALRLDGAKEPEKTLKPDDWNDRRDGRYQPQVGFNRNAPRGSLDQSGHRQVHHYVRGGGGGGGGYRGNSYDDRRGGGGGGGGYNDRQDFGRNYGGRDGGGPQRYHDQQQQRQGGYQGGGYGGGYGGGGGGGGGGGGGSYHQPYNQDQRRGGRGGGGGPPGYQRPPYRGGGGGGYHGNSSWR

Target ORF information:

RefSeq Version	NM_064557.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 5'-3' exoribonuclease 2 homolog (xrn-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064557.6

ORF Insert Sequence:

ATGGGAGTTC CCGCATTCTT CAGATGGTTG ACTAAAAAGT ATCCGGCAAC CGTGGTGAAT 
GCCAATGAAG ATCGTCAGCG AGACCAGGAT GGCAACCGGG TCCCCGTAGA TTGTACACAG 
CCCAATCCGA ATTTCCAGGA GTTTGATAAT CTATACCTGG ATATGAACGG TATTATCCAT 
CCGTGCACCC ATCCAGAAGA CAGGCCGGCT CCGAAGAACG AAGATGAGAT GTTCGCGCTC 
ATTTTCGAGT ACATCGATCG CATTTATTCG ATTGTCAGAC CGCGAAGGCT TCTCTATATG 
GCGATCGATG GAGTTGCTCC GCGAGCCAAG ATGAACCAGC AGCGAAGTCG TCGATTCCGG 
GCGAGTAAAG AGATGGCTGA AAAGGAGGCG AGCATCGAAG AGCAAAGAAA TCGATTGATG 
GCCGAAGGCA TCGCTGTGCC ACCGAAAAAG AAAGAAGAAG CTCATTTCGA TAGTAACTGT 
ATTACTCCTG GAACACCATT TATGGCTCGA CTCGCCGATG CTCTTCGATA TTATATTCAT 
GATCGGGTCA CTAATGACGC GTCATGGGCT AATATTGAGA TCATCCTCTC CGACGCGAAT 
GTTCCCGGCG AAGGCGAGCA CAAGATCATG GACTACGTCC GAAAGCAACG TGGCAACCCG 
GCTCACGACC CGAACACCGT CCACTGCCTC TGCGGAGCCG ACGCCGACCT TATTATGCTC 
GGAATCGCCA CCCACGAGGC CAACTTCAAT ATTATCCGAG AGGAGTTCGT GCCGAATCAG 
CCGCGGGCTT GTGATCTATG CGGTCAGTAC GGTCATGAGC TGAAGGAGTG TAGAGGCGCC 
GAAAATGAGA CCGATTTGGG CGATGACTAC TGTAAGCCGG AGCAGCGAGA GAAGAATTTC 
ATTTTTCTAC GGATCCCGGT GCTCAGGGAG TACTTGGAAA AGGAGCTCTC AATGCCGAAT 
CTTCCATTTA AATTCGACGT CGAGCGGGCT CTGGATGATT GGGTTTTCCT GTGCTTCTTC 
GTCGGAAACG ACTTCCTTCC ACATTTACCA TCGCTCGAGA TCCGAGAAGG GGCAATTGAC 
AGACTGATTA AGCTGTATAA GGAGATGGTC TACCAGATGA AAGGTTATCT GACAAAGGAC 
GGGATCCCGG AGCTCGATCG GGTCGAGATG ATTATGAAGG GGCTCGGACG CGTCGAAGAT 
GAGATTTTCA AGCGAAGACA GCAGGATGAG GAGCGATTCC AGGAAAATCA GCGAAACAAG 
AAAGCGAGAA TGCAGATGTA CGGAGGAGGA GGAAGAGGAG GTCGTGGTCG TGGTAGAGGA 
AGAGGTCAAC AACCGGCTTT TGTGCCGACG CACGGGATTC TGGCTCCGAT GGCGGCTCCG 
ATGCATCATT CGGGAGAGTC GACGAGGCAG ATGGCCTCGG AAGCCAGGCA GACGGCCATG 
AAGTTCACCA ACGATGCCAA TGAAACGGCT GCGGCGAATT TGAAGGCTTT GCTCAATGTG 
AAAGGTGAAG AATCGCCAGC AGACATTGCG TCAAGAAAAC GAAAAGCCGA GCAGCCACTG 
ATAAAGCCTG AAGAAGAGGA AGATGAAGGA CCAAAAGATG ACATTCGTCT CTACGAATCA 
GGCTGGAAGG ATCGATATTA CCGAGCGAAA TTCGACGTCG GATCCGACGA TATCGAGTTT 
CGGCACCGTG TCGCGTGGGC GTATGTGGAG GGTCTCTGCT GGGTACTACG GTACTATTAT 
CAAGGATGTG CCAGCTGGGA TTGGTATTTC CCGTATCATT ATGCACCGTT TGCCAGCGAT 
TTCGAGACTG TCGGAGAGTT TCAGCCCGAT TTTACGAGGC CTACCAAGCC GTTCAATCCG 
TTGGAGCAGC TGATGAGTGT TTTTCCGGCT GCGTCGAAAC AACATCTCCC AGTGGAATGG 
CAAAAGCTGA TGATTCAAGA CGATTCTCCA ATTATCGATC TCTATCCAGC AGATTTTCGC 
ATCGATCTCA ACGGAAAGAA GTACGCGTGG CAAGGAGTTG CTCTTCTGCC ATTTGTCGAT 
GAAACTCGCC TTCTCGCCAC CCTACAATCC GTCTATCCAA CACTGACCGC CGAGGAAAAG 
CAACGAAATA CACGTGGACC GAATCGAATT TTCATTGGAC GGAATCACAA GTCGTTCGAG 
TTCTTTCAAC AAGTCGCCGA GTCGAAATCC GATGATTTGG TTCCACTGGA TCCGACGCTT 
TTGAACGGAG TTAGCGGAAA AATCGCCTAT GATTCTACGG CAACAGCGCC CGGATTGCCG 
TTCGTATCGC CTGTCAATCA CGATGAATGC CAGGATTTGC CGACCAACTG CGGAATATGT 
GTGCTCTACG AAGATCCCGA GTACCCACAA GACTACATAT TCCCGGCACT CCGTCTCGAC 
GGAGCCAAGG AGCCCGAGAA AACGCTGAAA CCCGACGACT GGAATGATAG ACGAGACGGG 
CGGTATCAGC CGCAAGTGGG CTTCAATCGG AACGCGCCAC GTGGAAGCCT GGATCAATCG 
GGCCATCGAC AGGTGCATCA CTATGTGAGA GGTGGTGGTG GCGGCGGCGG CGGATACCGA 
GGAAATAGCT ATGATGATCG GAGAGGCGGT GGTGGTGGTG GTGGTGGATA CAACGATCGT 
CAAGATTTCG GCCGAAACTA CGGAGGAAGA GACGGTGGAG GTCCCCAGAG ATACCATGAT 
CAGCAGCAGC AGCGTCAAGG AGGCTATCAA GGAGGCGGAT ATGGAGGCGG TTATGGCGGC 
GGCGGCGGAG GTGGTGGTGG TGGTGGTGGA GGATCCTACC ACCAGCCGTA CAATCAGGAT 
CAGAGACGTG GAGGACGAGG TGGTGGTGGT GGGCCACCGG GATATCAGAG ACCACCGTAT 
CGAGGTGGCG GAGGTGGAGG CTATCACGGA AATTCATCAT GGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

xrn-2 ( NM_064557.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496958.2 Caenorhabditis elegans 5'-3' exoribonuclease 2 homolog (xrn-2), partial mRNA.

Price & Availability↑

CloneID	OCa337786
Clone ID Related Accession (Same CDS sequence)	NM_064557.5 , NM_064557.6
Accession Version	NM_064557.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	5'-3' exoribonuclease 2 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_064557.4. COMPLETENESS: incomplete on both ends.

ATGGGAGTTC CCGCATTCTT CAGATGGTTG ACTAAAAAGT ATCCGGCAAC CGTGGTGAAT 
GCCAATGAAG ATCGTCAGCG AGACCAGGAT GGCAACCGGG TCCCCGTAGA TTGTACACAG 
CCCAATCCGA ATTTCCAGGA GTTTGATAAT CTATACCTGG ATATGAACGG TATTATCCAT 
CCGTGCACCC ATCCAGAAGA CAGGCCGGCT CCGAAGAACG AAGATGAGAT GTTCGCGCTC 
ATTTTCGAGT ACATCGATCG CATTTATTCG ATTGTCAGAC CGCGAAGGCT TCTCTATATG 
GCGATCGATG GAGTTGCTCC GCGAGCCAAG ATGAACCAGC AGCGAAGTCG TCGATTCCGG 
GCGAGTAAAG AGATGGCTGA AAAGGAGGCG AGCATCGAAG AGCAAAGAAA TCGATTGATG 
GCCGAAGGCA TCGCTGTGCC ACCGAAAAAG AAAGAAGAAG CTCATTTCGA TAGTAACTGT 
ATTACTCCTG GAACACCATT TATGGCTCGA CTCGCCGATG CTCTTCGATA TTATATTCAT 
GATCGGGTCA CTAATGACGC GTCATGGGCT AATATTGAGA TCATCCTCTC CGACGCGAAT 
GTTCCCGGCG AAGGCGAGCA CAAGATCATG GACTACGTCC GAAAGCAACG TGGCAACCCG 
GCTCACGACC CGAACACCGT CCACTGCCTC TGCGGAGCCG ACGCCGACCT TATTATGCTC 
GGAATCGCCA CCCACGAGGC CAACTTCAAT ATTATCCGAG AGGAGTTCGT GCCGAATCAG 
CCGCGGGCTT GTGATCTATG CGGTCAGTAC GGTCATGAGC TGAAGGAGTG TAGAGGCGCC 
GAAAATGAGA CCGATTTGGG CGATGACTAC TGTAAGCCGG AGCAGCGAGA GAAGAATTTC 
ATTTTTCTAC GGATCCCGGT GCTCAGGGAG TACTTGGAAA AGGAGCTCTC AATGCCGAAT 
CTTCCATTTA AATTCGACGT CGAGCGGGCT CTGGATGATT GGGTTTTCCT GTGCTTCTTC 
GTCGGAAACG ACTTCCTTCC ACATTTACCA TCGCTCGAGA TCCGAGAAGG GGCAATTGAC 
AGACTGATTA AGCTGTATAA GGAGATGGTC TACCAGATGA AAGGTTATCT GACAAAGGAC 
GGGATCCCGG AGCTCGATCG GGTCGAGATG ATTATGAAGG GGCTCGGACG CGTCGAAGAT 
GAGATTTTCA AGCGAAGACA GCAGGATGAG GAGCGATTCC AGGAAAATCA GCGAAACAAG 
AAAGCGAGAA TGCAGATGTA CGGAGGAGGA GGAAGAGGAG GTCGTGGTCG TGGTAGAGGA 
AGAGGTCAAC AACCGGCTTT TGTGCCGACG CACGGGATTC TGGCTCCGAT GGCGGCTCCG 
ATGCATCATT CGGGAGAGTC GACGAGGCAG ATGGCCTCGG AAGCCAGGCA GACGGCCATG 
AAGTTCACCA ACGATGCCAA TGAAACGGCT GCGGCGAATT TGAAGGCTTT GCTCAATGTG 
AAAGGTGAAG AATCGCCAGC AGACATTGCG TCAAGAAAAC GAAAAGCCGA GCAGCCACTG 
ATAAAGCCTG AAGAAGAGGA AGATGAAGGA CCAAAAGATG ACATTCGTCT CTACGAATCA 
GGCTGGAAGG ATCGATATTA CCGAGCGAAA TTCGACGTCG GATCCGACGA TATCGAGTTT 
CGGCACCGTG TCGCGTGGGC GTATGTGGAG GGTCTCTGCT GGGTACTACG GTACTATTAT 
CAAGGATGTG CCAGCTGGGA TTGGTATTTC CCGTATCATT ATGCACCGTT TGCCAGCGAT 
TTCGAGACTG TCGGAGAGTT TCAGCCCGAT TTTACGAGGC CTACCAAGCC GTTCAATCCG 
TTGGAGCAGC TGATGAGTGT TTTTCCGGCT GCGTCGAAAC AACATCTCCC AGTGGAATGG 
CAAAAGCTGA TGATTCAAGA CGATTCTCCA ATTATCGATC TCTATCCAGC AGATTTTCGC 
ATCGATCTCA ACGGAAAGAA GTACGCGTGG CAAGGAGTTG CTCTTCTGCC ATTTGTCGAT 
GAAACTCGCC TTCTCGCCAC CCTACAATCC GTCTATCCAA CACTGACCGC CGAGGAAAAG 
CAACGAAATA CACGTGGACC GAATCGAATT TTCATTGGAC GGAATCACAA GTCGTTCGAG 
TTCTTTCAAC AAGTCGCCGA GTCGAAATCC GATGATTTGG TTCCACTGGA TCCGACGCTT 
TTGAACGGAG TTAGCGGAAA AATCGCCTAT GATTCTACGG CAACAGCGCC CGGATTGCCG 
TTCGTATCGC CTGTCAATCA CGATGAATGC CAGGATTTGC CGACCAACTG CGGAATATGT 
GTGCTCTACG AAGATCCCGA GTACCCACAA GACTACATAT TCCCGGCACT CCGTCTCGAC 
GGAGCCAAGG AGCCCGAGAA AACGCTGAAA CCCGACGACT GGAATGATAG ACGAGACGGG 
CGGTATCAGC CGCAAGTGGG CTTCAATCGG AACGCGCCAC GTGGAAGCCT GGATCAATCG 
GGCCATCGAC AGGTGCATCA CTATGTGAGA GGTGGTGGTG GCGGCGGCGG CGGATACCGA 
GGAAATAGCT ATGATGATCG GAGAGGCGGT GGTGGTGGTG GTGGTGGATA CAACGATCGT 
CAAGATTTCG GCCGAAACTA CGGAGGAAGA GACGGTGGAG GTCCCCAGAG ATACCATGAT 
CAGCAGCAGC AGCGTCAAGG AGGCTATCAA GGAGGCGGAT ATGGAGGCGG TTATGGCGGC 
GGCGGCGGAG GTGGTGGTGG TGGTGGTGGA GGATCCTACC ACCAGCCGTA CAATCAGGAT 
CAGAGACGTG GAGGACGAGG TGGTGGTGGT GGGCCACCGG GATATCAGAG ACCACCGTAT 
CGAGGTGGCG GAGGTGGAGG CTATCACGGA AATTCATCAT GGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496958.2
CDS	1..2928
Translation	MGVPAFFRWLTKKYPATVVNANEDRQRDQDGNRVPVDCTQPNPNFQEFDNLYLDMNGIIHPCTHPEDRPAPKNEDEMFALIFEYIDRIYSIVRPRRLLYMAIDGVAPRAKMNQQRSRRFRASKEMAEKEASIEEQRNRLMAEGIAVPPKKKEEAHFDSNCITPGTPFMARLADALRYYIHDRVTNDASWANIEIILSDANVPGEGEHKIMDYVRKQRGNPAHDPNTVHCLCGADADLIMLGIATHEANFNIIREEFVPNQPRACDLCGQYGHELKECRGAENETDLGDDYCKPEQREKNFIFLRIPVLREYLEKELSMPNLPFKFDVERALDDWVFLCFFVGNDFLPHLPSLEIREGAIDRLIKLYKEMVYQMKGYLTKDGIPELDRVEMIMKGLGRVEDEIFKRRQQDEERFQENQRNKKARMQMYGGGGRGGRGRGRGRGQQPAFVPTHGILAPMAAPMHHSGESTRQMASEARQTAMKFTNDANETAAANLKALLNVKGEESPADIASRKRKAEQPLIKPEEEEDEGPKDDIRLYESGWKDRYYRAKFDVGSDDIEFRHRVAWAYVEGLCWVLRYYYQGCASWDWYFPYHYAPFASDFETVGEFQPDFTRPTKPFNPLEQLMSVFPAASKQHLPVEWQKLMIQDDSPIIDLYPADFRIDLNGKKYAWQGVALLPFVDETRLLATLQSVYPTLTAEEKQRNTRGPNRIFIGRNHKSFEFFQQVAESKSDDLVPLDPTLLNGVSGKIAYDSTATAPGLPFVSPVNHDECQDLPTNCGICVLYEDPEYPQDYIFPALRLDGAKEPEKTLKPDDWNDRRDGRYQPQVGFNRNAPRGSLDQSGHRQVHHYVRGGGGGGGGYRGNSYDDRRGGGGGGGGYNDRQDFGRNYGGRDGGGPQRYHDQQQQRQGGYQGGGYGGGYGGGGGGGGGGGGGSYHQPYNQDQRRGGRGGGGGPPGYQRPPYRGGGGGGYHGNSSWR

Target ORF information:

RefSeq Version	NM_064557.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 5'-3' exoribonuclease 2 homolog (xrn-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064557.5

ORF Insert Sequence:

ATGGGAGTTC CCGCATTCTT CAGATGGTTG ACTAAAAAGT ATCCGGCAAC CGTGGTGAAT 
GCCAATGAAG ATCGTCAGCG AGACCAGGAT GGCAACCGGG TCCCCGTAGA TTGTACACAG 
CCCAATCCGA ATTTCCAGGA GTTTGATAAT CTATACCTGG ATATGAACGG TATTATCCAT 
CCGTGCACCC ATCCAGAAGA CAGGCCGGCT CCGAAGAACG AAGATGAGAT GTTCGCGCTC 
ATTTTCGAGT ACATCGATCG CATTTATTCG ATTGTCAGAC CGCGAAGGCT TCTCTATATG 
GCGATCGATG GAGTTGCTCC GCGAGCCAAG ATGAACCAGC AGCGAAGTCG TCGATTCCGG 
GCGAGTAAAG AGATGGCTGA AAAGGAGGCG AGCATCGAAG AGCAAAGAAA TCGATTGATG 
GCCGAAGGCA TCGCTGTGCC ACCGAAAAAG AAAGAAGAAG CTCATTTCGA TAGTAACTGT 
ATTACTCCTG GAACACCATT TATGGCTCGA CTCGCCGATG CTCTTCGATA TTATATTCAT 
GATCGGGTCA CTAATGACGC GTCATGGGCT AATATTGAGA TCATCCTCTC CGACGCGAAT 
GTTCCCGGCG AAGGCGAGCA CAAGATCATG GACTACGTCC GAAAGCAACG TGGCAACCCG 
GCTCACGACC CGAACACCGT CCACTGCCTC TGCGGAGCCG ACGCCGACCT TATTATGCTC 
GGAATCGCCA CCCACGAGGC CAACTTCAAT ATTATCCGAG AGGAGTTCGT GCCGAATCAG 
CCGCGGGCTT GTGATCTATG CGGTCAGTAC GGTCATGAGC TGAAGGAGTG TAGAGGCGCC 
GAAAATGAGA CCGATTTGGG CGATGACTAC TGTAAGCCGG AGCAGCGAGA GAAGAATTTC 
ATTTTTCTAC GGATCCCGGT GCTCAGGGAG TACTTGGAAA AGGAGCTCTC AATGCCGAAT 
CTTCCATTTA AATTCGACGT CGAGCGGGCT CTGGATGATT GGGTTTTCCT GTGCTTCTTC 
GTCGGAAACG ACTTCCTTCC ACATTTACCA TCGCTCGAGA TCCGAGAAGG GGCAATTGAC 
AGACTGATTA AGCTGTATAA GGAGATGGTC TACCAGATGA AAGGTTATCT GACAAAGGAC 
GGGATCCCGG AGCTCGATCG GGTCGAGATG ATTATGAAGG GGCTCGGACG CGTCGAAGAT 
GAGATTTTCA AGCGAAGACA GCAGGATGAG GAGCGATTCC AGGAAAATCA GCGAAACAAG 
AAAGCGAGAA TGCAGATGTA CGGAGGAGGA GGAAGAGGAG GTCGTGGTCG TGGTAGAGGA 
AGAGGTCAAC AACCGGCTTT TGTGCCGACG CACGGGATTC TGGCTCCGAT GGCGGCTCCG 
ATGCATCATT CGGGAGAGTC GACGAGGCAG ATGGCCTCGG AAGCCAGGCA GACGGCCATG 
AAGTTCACCA ACGATGCCAA TGAAACGGCT GCGGCGAATT TGAAGGCTTT GCTCAATGTG 
AAAGGTGAAG AATCGCCAGC AGACATTGCG TCAAGAAAAC GAAAAGCCGA GCAGCCACTG 
ATAAAGCCTG AAGAAGAGGA AGATGAAGGA CCAAAAGATG ACATTCGTCT CTACGAATCA 
GGCTGGAAGG ATCGATATTA CCGAGCGAAA TTCGACGTCG GATCCGACGA TATCGAGTTT 
CGGCACCGTG TCGCGTGGGC GTATGTGGAG GGTCTCTGCT GGGTACTACG GTACTATTAT 
CAAGGATGTG CCAGCTGGGA TTGGTATTTC CCGTATCATT ATGCACCGTT TGCCAGCGAT 
TTCGAGACTG TCGGAGAGTT TCAGCCCGAT TTTACGAGGC CTACCAAGCC GTTCAATCCG 
TTGGAGCAGC TGATGAGTGT TTTTCCGGCT GCGTCGAAAC AACATCTCCC AGTGGAATGG 
CAAAAGCTGA TGATTCAAGA CGATTCTCCA ATTATCGATC TCTATCCAGC AGATTTTCGC 
ATCGATCTCA ACGGAAAGAA GTACGCGTGG CAAGGAGTTG CTCTTCTGCC ATTTGTCGAT 
GAAACTCGCC TTCTCGCCAC CCTACAATCC GTCTATCCAA CACTGACCGC CGAGGAAAAG 
CAACGAAATA CACGTGGACC GAATCGAATT TTCATTGGAC GGAATCACAA GTCGTTCGAG 
TTCTTTCAAC AAGTCGCCGA GTCGAAATCC GATGATTTGG TTCCACTGGA TCCGACGCTT 
TTGAACGGAG TTAGCGGAAA AATCGCCTAT GATTCTACGG CAACAGCGCC CGGATTGCCG 
TTCGTATCGC CTGTCAATCA CGATGAATGC CAGGATTTGC CGACCAACTG CGGAATATGT 
GTGCTCTACG AAGATCCCGA GTACCCACAA GACTACATAT TCCCGGCACT CCGTCTCGAC 
GGAGCCAAGG AGCCCGAGAA AACGCTGAAA CCCGACGACT GGAATGATAG ACGAGACGGG 
CGGTATCAGC CGCAAGTGGG CTTCAATCGG AACGCGCCAC GTGGAAGCCT GGATCAATCG 
GGCCATCGAC AGGTGCATCA CTATGTGAGA GGTGGTGGTG GCGGCGGCGG CGGATACCGA 
GGAAATAGCT ATGATGATCG GAGAGGCGGT GGTGGTGGTG GTGGTGGATA CAACGATCGT 
CAAGATTTCG GCCGAAACTA CGGAGGAAGA GACGGTGGAG GTCCCCAGAG ATACCATGAT 
CAGCAGCAGC AGCGTCAAGG AGGCTATCAA GGAGGCGGAT ATGGAGGCGG TTATGGCGGC 
GGCGGCGGAG GTGGTGGTGG TGGTGGTGGA GGATCCTACC ACCAGCCGTA CAATCAGGAT 
CAGAGACGTG GAGGACGAGG TGGTGGTGGT GGGCCACCGG GATATCAGAG ACCACCGTAT 
CGAGGTGGCG GAGGTGGAGG CTATCACGGA AATTCATCAT GGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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xrn-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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