MLH3 cDNA ORF clone, Bos taurus(cattle)

The following MLH3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MLH3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa219982 XM_024998144.1
Latest version!
Bos taurus mutL homolog 3 (MLH3), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa219983 XM_024998145.1
Latest version!
Bos taurus mutL homolog 3 (MLH3), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa219981 XM_024998142.1
Latest version!
Bos taurus mutL homolog 3 (MLH3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa57927 XM_024998143.1
Latest version!
Bos taurus mutL homolog 3 (MLH3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa57924 XM_010813168.2
Latest version!
Bos taurus mutL homolog 3 (MLH3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa57924 XM_010809605.3
Latest version!
Bos taurus mutL homolog 3 (MLH3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa57925 XM_010813188.2
Latest version!
Bos taurus mutL homolog 3 (MLH3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa57925 XM_010813172.2
Latest version!
Bos taurus mutL homolog 3 (MLH3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa57925 XM_010809606.3
Latest version!
Bos taurus mutL homolog 3 (MLH3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa57925 XM_010809611.3
Latest version!
Bos taurus mutL homolog 3 (MLH3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa57926 XM_010813177.2
Latest version!
Bos taurus mutL homolog 3 (MLH3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa57926 XM_010809607.3
Latest version!
Bos taurus mutL homolog 3 (MLH3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa57925 XM_010809611.2 Bos taurus mutL homolog 3 (MLH3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa57926 XM_010809607.2 Bos taurus mutL homolog 3 (MLH3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa57924 XM_010809605.2 Bos taurus mutL homolog 3 (MLH3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa57925 XM_010809606.2 Bos taurus mutL homolog 3 (MLH3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa219982
    Clone ID Related Accession (Same CDS sequence) XM_024998144.1
    Accession Version XM_024998144.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1836bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGATC TCAGTACAGG TGTCATCCCT TTAGAAGCGA CCTTGTTCTT
    CCTTTCCTTC CTAGAGCTCG GGAAGAGAGG ACTGTGATGA GACAGAATAA CAGAGCTACT
    GGGGACGATG CCTTTGGTCC TGAATCACTT CAATCTTTGT TCTCAGAATG GGACAATCCA
    GTATTTGCCC GTTATCCAGA GGTTGCTGTT GACGTAAGCA GCGGCCAAGC TGAGAGCCTA
    GCAGTTAAAA TTCACAACAT CTTGTATCCT TATCGTTTCA CCAAAGAAAT GATTCACTCA
    ATGCAGGTTC TCCAGCAAGT GGATAACAAG TTTATTGCCT GTTTAATGAG CACTAAGACT
    GAAGAGAATG GTGAGGCAGG TGGAAACCTG CTAGTCTTGG TGGACCAGCA CGCTGCCCAC
    GAGCGCATCC GATTGGAGCA GCTGATCATT GATTCCTATG AAAAGCAACA GCCACAAGGC
    TTTGGTCGAA AAAAATTACT GTCTTCCATT GTAAGTCCTC CACTAGAGAT AACAGTGACA
    GAAGAACAAA GGAGACTCTT ACGGTGTTAC CACAAAAATC TGGAAGATCT GGGCCTTGAA
    ATTGTATTTC CAGACACCAG TGATTCACTG GTCCTCATAG GAAAAGTACC ACTCTGTTTT
    GTAGAAAGAG AAGCAAGTGA ACTTCGAAGA GGAAGATCTA CTGTGACCAA GGGCATTGTG
    GAGGAGTTTA TTCGAGAACA AGTGGAGCTA CTCCAGACCA CAGGAGGCAT CCAAGGGACT
    TTGCCACTGA CTGTCCAGAA GGTGTTGGCA TCCCAAGCCT GCCATGGGGC CATTAAGTTT
    AACGATGGCC TGAGCTTCGA GGAGAGCTGC CGCCTTATTG AAGCGCTGTC CTGGTGCCAG
    CTGCCGTTCC AGTGTGCTCA TGGCAGGCCC TCTATGCTGC CATTAGCTAA CATCGACCAC
    TTGGAGCAGG AAAAACAGAC TAAACCCAAT CTTGCTAAAC TTCGCAGAAT GGCTCAAGCC
    TGGCATCTCT TTGGAAAGGA GAAGGATGTG ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853912.1
    CDS57..1892
    Translation

    Target ORF information:

    RefSeq Version XM_024998144.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998144.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGATC TCAGTACAGG TGTCATCCCT TTAGAAGCGA CCTTGTTCTT
    CCTTTCCTTC CTAGAGCTCG GGAAGAGAGG ACTGTGATGA GACAGAATAA CAGAGCTACT
    GGGGACGATG CCTTTGGTCC TGAATCACTT CAATCTTTGT TCTCAGAATG GGACAATCCA
    GTATTTGCCC GTTATCCAGA GGTTGCTGTT GACGTAAGCA GCGGCCAAGC TGAGAGCCTA
    GCAGTTAAAA TTCACAACAT CTTGTATCCT TATCGTTTCA CCAAAGAAAT GATTCACTCA
    ATGCAGGTTC TCCAGCAAGT GGATAACAAG TTTATTGCCT GTTTAATGAG CACTAAGACT
    GAAGAGAATG GTGAGGCAGG TGGAAACCTG CTAGTCTTGG TGGACCAGCA CGCTGCCCAC
    GAGCGCATCC GATTGGAGCA GCTGATCATT GATTCCTATG AAAAGCAACA GCCACAAGGC
    TTTGGTCGAA AAAAATTACT GTCTTCCATT GTAAGTCCTC CACTAGAGAT AACAGTGACA
    GAAGAACAAA GGAGACTCTT ACGGTGTTAC CACAAAAATC TGGAAGATCT GGGCCTTGAA
    ATTGTATTTC CAGACACCAG TGATTCACTG GTCCTCATAG GAAAAGTACC ACTCTGTTTT
    GTAGAAAGAG AAGCAAGTGA ACTTCGAAGA GGAAGATCTA CTGTGACCAA GGGCATTGTG
    GAGGAGTTTA TTCGAGAACA AGTGGAGCTA CTCCAGACCA CAGGAGGCAT CCAAGGGACT
    TTGCCACTGA CTGTCCAGAA GGTGTTGGCA TCCCAAGCCT GCCATGGGGC CATTAAGTTT
    AACGATGGCC TGAGCTTCGA GGAGAGCTGC CGCCTTATTG AAGCGCTGTC CTGGTGCCAG
    CTGCCGTTCC AGTGTGCTCA TGGCAGGCCC TCTATGCTGC CATTAGCTAA CATCGACCAC
    TTGGAGCAGG AAAAACAGAC TAAACCCAAT CTTGCTAAAC TTCGCAGAAT GGCTCAAGCC
    TGGCATCTCT TTGGAAAGGA GAAGGATGTG ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219983
    Clone ID Related Accession (Same CDS sequence) XM_024998145.1
    Accession Version XM_024998145.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGCTAC TGGGGACGAT GCCTTTGGTC CTGAATCACT TCAATCTTTG
    TTCTCAGAAT GGGACAATCC AGTATTTGCC CGTTATCCAG AGGTTGCTGT TGACGTAAGC
    AGCGGCCAAG CTGAGAGCCT AGCAGTTAAA ATTCACAACA TCTTGTATCC TTATCGTTTC
    ACCAAAGAAA TGATTCACTC AATGCAGGTT CTCCAGCAAG TGGATAACAA GTTTATTGCC
    TGTTTAATGA GCACTAAGAC TGAAGAGAAT GGTGAGGCAG GTGGAAACCT GCTAGTCTTG
    GTGGACCAGC ACGCTGCCCA CGAGCGCATC CGATTGGAGC AGCTGATCAT TGATTCCTAT
    GAAAAGCAAC AGCCACAAGG CTTTGGTCGA AAAAAATTAC TGTCTTCCAT TGTAAGTCCT
    CCACTAGAGA TAACAGTGAC AGAAGAACAA AGGAGACTCT TACGGTGTTA CCACAAAAAT
    CTGGAAGATC TGGGCCTTGA AATTGTATTT CCAGACACCA GTGATTCACT GGTCCTCATA
    GGAAAAGTAC CACTCTGTTT TGTAGAAAGA GAAGCAAGTG AACTTCGAAG AGGAAGATCT
    ACTGTGACCA AGGGCATTGT GGAGGAGTTT ATTCGAGAAC AAGTGGAGCT ACTCCAGACC
    ACAGGAGGCA TCCAAGGGAC TTTGCCACTG ACTGTCCAGA AGGTGTTGGC ATCCCAAGCC
    TGCCATGGGG CCATTAAGTT TAACGATGGC CTGAGCTTCG AGGAGAGCTG CCGCCTTATT
    GAAGCGCTGT CCTGGTGCCA GCTGCCGTTC CAGTGTGCTC ATGGCAGGCC CTCTATGCTG
    CCATTAGCTA ACATCGACCA CTTGGAGCAG GAAAAACAGA CTAAACCCAA TCTTGCTAAA
    CTTCGCAGAA TGGCTCAAGC CTGGCATCTC TTTGGAAAGG AGAAGGATGT GATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853913.1
    CDS57..1793
    Translation

    Target ORF information:

    RefSeq Version XM_024998145.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998145.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGCTAC TGGGGACGAT GCCTTTGGTC CTGAATCACT TCAATCTTTG
    TTCTCAGAAT GGGACAATCC AGTATTTGCC CGTTATCCAG AGGTTGCTGT TGACGTAAGC
    AGCGGCCAAG CTGAGAGCCT AGCAGTTAAA ATTCACAACA TCTTGTATCC TTATCGTTTC
    ACCAAAGAAA TGATTCACTC AATGCAGGTT CTCCAGCAAG TGGATAACAA GTTTATTGCC
    TGTTTAATGA GCACTAAGAC TGAAGAGAAT GGTGAGGCAG GTGGAAACCT GCTAGTCTTG
    GTGGACCAGC ACGCTGCCCA CGAGCGCATC CGATTGGAGC AGCTGATCAT TGATTCCTAT
    GAAAAGCAAC AGCCACAAGG CTTTGGTCGA AAAAAATTAC TGTCTTCCAT TGTAAGTCCT
    CCACTAGAGA TAACAGTGAC AGAAGAACAA AGGAGACTCT TACGGTGTTA CCACAAAAAT
    CTGGAAGATC TGGGCCTTGA AATTGTATTT CCAGACACCA GTGATTCACT GGTCCTCATA
    GGAAAAGTAC CACTCTGTTT TGTAGAAAGA GAAGCAAGTG AACTTCGAAG AGGAAGATCT
    ACTGTGACCA AGGGCATTGT GGAGGAGTTT ATTCGAGAAC AAGTGGAGCT ACTCCAGACC
    ACAGGAGGCA TCCAAGGGAC TTTGCCACTG ACTGTCCAGA AGGTGTTGGC ATCCCAAGCC
    TGCCATGGGG CCATTAAGTT TAACGATGGC CTGAGCTTCG AGGAGAGCTG CCGCCTTATT
    GAAGCGCTGT CCTGGTGCCA GCTGCCGTTC CAGTGTGCTC ATGGCAGGCC CTCTATGCTG
    CCATTAGCTA ACATCGACCA CTTGGAGCAG GAAAAACAGA CTAAACCCAA TCTTGCTAAA
    CTTCGCAGAA TGGCTCAAGC CTGGCATCTC TTTGGAAAGG AGAAGGATGT GATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219981
    Clone ID Related Accession (Same CDS sequence) XM_024998142.1
    Accession Version XM_024998142.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGTAA GTCCCAAAAG TTAAGAGAAA TAAATTTTAA ATATAAGGAG
    TTTGAGCTTA CTGGCTTTAT CAGCTCCGAA GCACATTATA ATAAGAATAT GCAGTTTTTG
    TTTGTGAACA AGAGGTTAGT TTTAAGGACA AAGTTGCATA AACTCATTGA CTTTTTATTA
    AGGAAAGAAA GTATTATATG CAAACCAAAG AGTGGTTCTG CCAGTAGGCA AGTGAATTCA
    AATCCTCGGC CTCGGTCTAA CCCGGAACTC CATGGTATCT ATGTAATCAA TGTGCAGTGC
    CAGTTCTGTG AGTATGACGT ATGCCTGGAG CCAGCAAAAA CCCTGATTGA GTTTCAGAAC
    TGGGATACTC TTTTGATTTG CATTCAGGAA GGAGTAAAAA CGTTTTTAAA GAAAGAAAAA
    TTATTTGTGG AATTATCAAG TGAAGACATT AAGGAATTTA GTGAAGATAA TGATTTTAGC
    TTGTTTAGTG CCAGTCTTCA GAAGCAAGTG TCCTCTGATG AGAAGTGTGG GCAGGTCAGT
    TTCCAAGAAG CATGTAATAA TATTTTGGAT TCCTACGAGA TGTTCAATTT GCAGTCAAAA
    GCTGTGAAAA GAAAAGCTCC TGTAGAAAAC ATAAGCACAC AAAATTCTAG GGATTCAGAA
    GCTATCAGAA AAAAGACAAA TGATTCATTT TTGTACACTT ACAAATCTGA TGGGCCAGCC
    CATTGTAAAA TGGCAGAGTC ATCTTTGCAA AATGAAGACA GTGCTTGCTC ACAGTCAAGG
    AACCTAGAAC AAGAGACAGC TGGAGCATCA GAATTGGGAG AAAATAAGAA ACATAAAAAA
    TCTTGCTTGG AACATAACTC TTCAGAAAAT CCATGTGGAA CCACTTCAGA AATGTTTGGA
    AGCCCTTTTC AGACATTATA TCACTTTGAG GGGAGTGGAG AAGATCTAGA AATACAGAAA
    GTAAGTACTA CTGTTAATAG CATGGCTGCC AACATCCTGG AAAATAACAG AATTCAGAAT
    CAACCAGAGA GATTTAAAGA TGCTACTGAA ATGGATTGCC AACCTCTGTC TTTTGAGACA
    GCATTATCAG GAGAACCTAG TGCTCAGAGA GAGGAAGAGA AAAGAAAAAA ACCTAGTACT
    TGTGGAAGAA TAAATGTTTT TCGTTATGGA CAAGTTAAAT TATGTTCCAC TGGCTTCATA
    ACTCATGTGG TACAGAATGA GCAAACTAAA TTAACTGAAA CAGAACATTT ATTTAAAAGT
    TATGCTCAAC CTGGTCCTGT GAGTGCCAAA GAAACATTTA GAAATAGAAC ACACCATTCA
    GTTGAGACTC CCAACAGCAA AGATTTAACC AGCACTTTAA GTAAAGAATT TGCTGAACTA
    CCCAACAAAA GTCTGTACAG CACAAATCGA AGTTATGAGC TAGAGAACAA ACATACAGCA
    GCCTGTAAAA ATTTTGCTAT TTTTCAGGAA AGTGGTAAAA AATCACACAC AGGTGGCTTA
    TTACCTAACA CATCCTCTTT TTCTTGGGGT GGACATGTTT CAGATGGTAG TAAGAAAACA
    GACAAGTTGA TTGGTCCTGC CGAACCCATA CCTCATAAGA AGCTAAGCTT AAGCTCACAA
    CTAGGATCTT TAGAGAAGTT TAAGAGGCAA TATGGGAAGG TTAAAAATCC TCTGAATACT
    GAAGTGGAGG AAAATAATAC TTTAGAAATC ACTACCAATC TCAGTCCTCA GGTTGAAGCT
    GACATTCCAT GGAAAGACAA AAACCACTTA GACAACTCTG ACATCTGTGA AATCACTACT
    ATGAAATATA ATGATTCCTA TGGTAGTTGT CAACCAGTAA ATCACATGTT TTCTTCAGAA
    AAGCTTCCAT TCTCCAAGGA AGATGATTGT TTGGAACAAC AGATGCTTTG CTTAACAGAA
    AGCACTGTAA CTCTACAGGA GTTACCTCAT TTTAACAGTA AATTTTTGGA TGTTGAGAAG
    TCACCTGAAT CACTAGCCTC TAAATTATCC AGATTGAAGG GTTCCGAAAG AGAGACTCAA
    AGGATGGATG TGAATCATTT TAATGAATTG CTACAATTAG ATTCCAGTAG GAAAGATGGT
    GACTTGTCCA GTGGGTTAAC CCTAGATTCT TGTAAGTTAT TTAAAAATGA GCATAAAAAC
    CCAGAAAGTG ACAGTATCCC AATGTCAGAC TCTGTCACAC AAGGTAATCT CTTCAGTAAA
    GACAGTGAAA CATATTCTAA CAACAATACC ACTGAGAAGA TACCAGAAAA TCCTTTGGTA
    CTGCCCTATG ACCATGCTAC AGTTATCAGT AAGGATTCAG ATATTCTTAT AGCTTCAAAA
    CAACAGATTG GAAGTCCTGA CTCTCTGAGT AGAATGTTAA TGAGTCGCGT GGAAGATTCC
    ACAGCTGACC AAAATGGAAC TTGTTTTCAG AGTGAGGAAT CCATAGCAAG AACTTGTTCT
    GAAAATGAAG AGTCCAACAT ATGTTCTTTG GATTGGCAGC AGCATTTTGA TGTAACTCTG
    GGAAGAATGG TTTACATCAA CAAACTAACA GGACTTAGCA CATTCACTGC TCCTACTGAG
    GATGTTCAGG CTGCTTGTAC TAAAGACCTG ACAACTATGG CTGTGGATGT TCTACTGGAG
    AATGCTACTG GGGACGATGC CTTTGGTCCT GAATCACTTC AATCTTTGTT CTCAGAATGG
    GACAATCCAG TATTTGCCCG TTATCCAGAG GTTGCTGTTG ACGTAAGCAG CGGCCAAGCT
    GAGAGCCTAG CAGTTAAAAT TCACAACATC TTGTATCCTT ATCGTTTCAC CAAAGAAATG
    ATTCACTCAA TGCAGGTTCT CCAGCAAGTG GATAACAAGT TTATTGCCTG TTTAATGAGC
    ACTAAGACTG AAGAGAATGG TGAGGCAGGT GGAAACCTGC TAGTCTTGGT GGACCAGCAC
    GCTGCCCACG AGCGCATCCG ATTGGAGCAG CTGATCATTG ATTCCTATGA AAAGCAACAG
    CCACAAGGCT TTGGTCGAAA AAAATTACTG TCTTCCATTG TAAGTCCTCC ACTAGAGATA
    ACAGTGACAG AAGAACAAAG GAGACTCTTA CGGTGTTACC ACAAAAATCT GGAAGATCTG
    GGCCTTGAAA TTGTATTTCC AGACACCAGT GATTCACTGG TCCTCATAGG AAAAGTACCA
    CTCTGTTTTG TAGAAAGAGA AGCAAGTGAA CTTCGAAGAG GAAGATCTAC TGTGACCAAG
    GGCATTGTGG AGGAGTTTAT TCGAGAACAA GTGGAGCTAC TCCAGACCAC AGGAGGCATC
    CAAGGGACTT TGCCACTGAC TGTCCAGAAG GTGTTGGCAT CCCAAGCCTG CCATGGGGCC
    ATTAAGTTTA ACGATGGCCT GAGCTTCGAG GAGAGCTGCC GCCTTATTGA AGCGCTGTCC
    TGGTGCCAGC TGCCGTTCCA GTGTGCTCAT GGCAGGCCCT CTATGCTGCC ATTAGCTAAC
    ATCGACCACT TGGAGCAGGA AAAACAGACT AAACCCAATC TTGCTAAACT TCGCAGAATG
    GCTCAAGCCT GGCATCTCTT TGGAAAGGAG AAGGATGTGA TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853910.1
    CDS60..4424
    Translation

    Target ORF information:

    RefSeq Version XM_024998142.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998142.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGTAA GTCCCAAAAG TTAAGAGAAA TAAATTTTAA ATATAAGGAG
    TTTGAGCTTA CTGGCTTTAT CAGCTCCGAA GCACATTATA ATAAGAATAT GCAGTTTTTG
    TTTGTGAACA AGAGGTTAGT TTTAAGGACA AAGTTGCATA AACTCATTGA CTTTTTATTA
    AGGAAAGAAA GTATTATATG CAAACCAAAG AGTGGTTCTG CCAGTAGGCA AGTGAATTCA
    AATCCTCGGC CTCGGTCTAA CCCGGAACTC CATGGTATCT ATGTAATCAA TGTGCAGTGC
    CAGTTCTGTG AGTATGACGT ATGCCTGGAG CCAGCAAAAA CCCTGATTGA GTTTCAGAAC
    TGGGATACTC TTTTGATTTG CATTCAGGAA GGAGTAAAAA CGTTTTTAAA GAAAGAAAAA
    TTATTTGTGG AATTATCAAG TGAAGACATT AAGGAATTTA GTGAAGATAA TGATTTTAGC
    TTGTTTAGTG CCAGTCTTCA GAAGCAAGTG TCCTCTGATG AGAAGTGTGG GCAGGTCAGT
    TTCCAAGAAG CATGTAATAA TATTTTGGAT TCCTACGAGA TGTTCAATTT GCAGTCAAAA
    GCTGTGAAAA GAAAAGCTCC TGTAGAAAAC ATAAGCACAC AAAATTCTAG GGATTCAGAA
    GCTATCAGAA AAAAGACAAA TGATTCATTT TTGTACACTT ACAAATCTGA TGGGCCAGCC
    CATTGTAAAA TGGCAGAGTC ATCTTTGCAA AATGAAGACA GTGCTTGCTC ACAGTCAAGG
    AACCTAGAAC AAGAGACAGC TGGAGCATCA GAATTGGGAG AAAATAAGAA ACATAAAAAA
    TCTTGCTTGG AACATAACTC TTCAGAAAAT CCATGTGGAA CCACTTCAGA AATGTTTGGA
    AGCCCTTTTC AGACATTATA TCACTTTGAG GGGAGTGGAG AAGATCTAGA AATACAGAAA
    GTAAGTACTA CTGTTAATAG CATGGCTGCC AACATCCTGG AAAATAACAG AATTCAGAAT
    CAACCAGAGA GATTTAAAGA TGCTACTGAA ATGGATTGCC AACCTCTGTC TTTTGAGACA
    GCATTATCAG GAGAACCTAG TGCTCAGAGA GAGGAAGAGA AAAGAAAAAA ACCTAGTACT
    TGTGGAAGAA TAAATGTTTT TCGTTATGGA CAAGTTAAAT TATGTTCCAC TGGCTTCATA
    ACTCATGTGG TACAGAATGA GCAAACTAAA TTAACTGAAA CAGAACATTT ATTTAAAAGT
    TATGCTCAAC CTGGTCCTGT GAGTGCCAAA GAAACATTTA GAAATAGAAC ACACCATTCA
    GTTGAGACTC CCAACAGCAA AGATTTAACC AGCACTTTAA GTAAAGAATT TGCTGAACTA
    CCCAACAAAA GTCTGTACAG CACAAATCGA AGTTATGAGC TAGAGAACAA ACATACAGCA
    GCCTGTAAAA ATTTTGCTAT TTTTCAGGAA AGTGGTAAAA AATCACACAC AGGTGGCTTA
    TTACCTAACA CATCCTCTTT TTCTTGGGGT GGACATGTTT CAGATGGTAG TAAGAAAACA
    GACAAGTTGA TTGGTCCTGC CGAACCCATA CCTCATAAGA AGCTAAGCTT AAGCTCACAA
    CTAGGATCTT TAGAGAAGTT TAAGAGGCAA TATGGGAAGG TTAAAAATCC TCTGAATACT
    GAAGTGGAGG AAAATAATAC TTTAGAAATC ACTACCAATC TCAGTCCTCA GGTTGAAGCT
    GACATTCCAT GGAAAGACAA AAACCACTTA GACAACTCTG ACATCTGTGA AATCACTACT
    ATGAAATATA ATGATTCCTA TGGTAGTTGT CAACCAGTAA ATCACATGTT TTCTTCAGAA
    AAGCTTCCAT TCTCCAAGGA AGATGATTGT TTGGAACAAC AGATGCTTTG CTTAACAGAA
    AGCACTGTAA CTCTACAGGA GTTACCTCAT TTTAACAGTA AATTTTTGGA TGTTGAGAAG
    TCACCTGAAT CACTAGCCTC TAAATTATCC AGATTGAAGG GTTCCGAAAG AGAGACTCAA
    AGGATGGATG TGAATCATTT TAATGAATTG CTACAATTAG ATTCCAGTAG GAAAGATGGT
    GACTTGTCCA GTGGGTTAAC CCTAGATTCT TGTAAGTTAT TTAAAAATGA GCATAAAAAC
    CCAGAAAGTG ACAGTATCCC AATGTCAGAC TCTGTCACAC AAGGTAATCT CTTCAGTAAA
    GACAGTGAAA CATATTCTAA CAACAATACC ACTGAGAAGA TACCAGAAAA TCCTTTGGTA
    CTGCCCTATG ACCATGCTAC AGTTATCAGT AAGGATTCAG ATATTCTTAT AGCTTCAAAA
    CAACAGATTG GAAGTCCTGA CTCTCTGAGT AGAATGTTAA TGAGTCGCGT GGAAGATTCC
    ACAGCTGACC AAAATGGAAC TTGTTTTCAG AGTGAGGAAT CCATAGCAAG AACTTGTTCT
    GAAAATGAAG AGTCCAACAT ATGTTCTTTG GATTGGCAGC AGCATTTTGA TGTAACTCTG
    GGAAGAATGG TTTACATCAA CAAACTAACA GGACTTAGCA CATTCACTGC TCCTACTGAG
    GATGTTCAGG CTGCTTGTAC TAAAGACCTG ACAACTATGG CTGTGGATGT TCTACTGGAG
    AATGCTACTG GGGACGATGC CTTTGGTCCT GAATCACTTC AATCTTTGTT CTCAGAATGG
    GACAATCCAG TATTTGCCCG TTATCCAGAG GTTGCTGTTG ACGTAAGCAG CGGCCAAGCT
    GAGAGCCTAG CAGTTAAAAT TCACAACATC TTGTATCCTT ATCGTTTCAC CAAAGAAATG
    ATTCACTCAA TGCAGGTTCT CCAGCAAGTG GATAACAAGT TTATTGCCTG TTTAATGAGC
    ACTAAGACTG AAGAGAATGG TGAGGCAGGT GGAAACCTGC TAGTCTTGGT GGACCAGCAC
    GCTGCCCACG AGCGCATCCG ATTGGAGCAG CTGATCATTG ATTCCTATGA AAAGCAACAG
    CCACAAGGCT TTGGTCGAAA AAAATTACTG TCTTCCATTG TAAGTCCTCC ACTAGAGATA
    ACAGTGACAG AAGAACAAAG GAGACTCTTA CGGTGTTACC ACAAAAATCT GGAAGATCTG
    GGCCTTGAAA TTGTATTTCC AGACACCAGT GATTCACTGG TCCTCATAGG AAAAGTACCA
    CTCTGTTTTG TAGAAAGAGA AGCAAGTGAA CTTCGAAGAG GAAGATCTAC TGTGACCAAG
    GGCATTGTGG AGGAGTTTAT TCGAGAACAA GTGGAGCTAC TCCAGACCAC AGGAGGCATC
    CAAGGGACTT TGCCACTGAC TGTCCAGAAG GTGTTGGCAT CCCAAGCCTG CCATGGGGCC
    ATTAAGTTTA ACGATGGCCT GAGCTTCGAG GAGAGCTGCC GCCTTATTGA AGCGCTGTCC
    TGGTGCCAGC TGCCGTTCCA GTGTGCTCAT GGCAGGCCCT CTATGCTGCC ATTAGCTAAC
    ATCGACCACT TGGAGCAGGA AAAACAGACT AAACCCAATC TTGCTAAACT TCGCAGAATG
    GCTCAAGCCT GGCATCTCTT TGGAAAGGAG AAGGATGTGA TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57927
    Clone ID Related Accession (Same CDS sequence) XM_024998143.1
    Accession Version XM_024998143.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGATCAAAT GCTTGTCAGC TGAAGTACAA GCTAGATTGC GTTCTGGTTT GGCTGTATGC 
    TCCTTAGGCC AATGTGTTGA GGAGCTTGCC CTGAACAGTA TTGATGCTGA AGCAAAGTGT
    GTGGCTGTCA GGGTGAACAT GGAAACCTTT CAAGTTCAAG TGATAGACAA TGGATTTGGG
    ATGGGGAGTG ATGATGTAGA CAAGGTGGGA AATCGTTATT TCACCAGTAA ATGCAACTCC
    ATACAGGACT TGGAGAACCC AAGGTTTTAT GGTTTCCGGG GGGAGGCGTT GGCCAGTATA
    GCTGATATGG CCAGTGCTGT GGAAATTTCA TCCAAGAAAA ACAAGTCGAT GAAAACATTT
    GTGAAACTGT TTCAGAATGG GAAAGCCCTG AAAGCTTGTG AAGCTGACTT AACTCGACCA
    AGTGCCGGGA CGACAGTCAC TGTATATAAT CTGTTTTATC AGTTACCTGT TAGGAGGAAA
    TGCATGGATC CTAGACTGGA GTTTGAGAAG GTTAGGCAGA GGATAGAAGC TCTGTCACTC
    ATGCACCCTA CCATTTCTTT CTCTTTGAGA AATGATGTTT CTGGTTCCAT GGTTCTTCAA
    CTCCCTAAAA CCAAAGACAT ATGTTCCCGA TTTTGTCAAA TTTATGGACT GGGTAAGTCC
    CAAAAGTTAA GAGAAATAAA TTTTAAATAT AAGGAGTTTG AGCTTACTGG CTTTATCAGC
    TCCGAAGCAC ATTATAATAA GAATATGCAG TTTTTGTTTG TGAACAAGAG GTTAGTTTTA
    AGGACAAAGT TGCATAAACT CATTGACTTT TTATTAAGGA AAGAAAGTAT TATATGCAAA
    CCAAAGAGTG GTTCTGCCAG TAGGCAAGTG AATTCAAATC CTCGGCCTCG GTCTAACCCG
    GAACTCCATG GTATCTATGT AATCAATGTG CAGTGCCAGT TCTGTGAGTA TGACGTATGC
    CTGGAGCCAG CAAAAACCCT GATTGAGTTT CAGAACTGGG ATACTCTTTT GATTTGCATT
    CAGGAAGGAG TAAAAACGTT TTTAAAGAAA GAAAAATTAT TTGTGGAATT ATCAAGTGAA
    GACATTAAGG AATTTAGTGA AGATAATGAT TTTAGCTTGT TTAGTGCCAG TCTTCAGAAG
    CAAGTGTCCT CTGATGAGAA GTGTGGGCAG GTCAGTTTCC AAGAAGCATG TAATAATATT
    TTGGATTCCT ACGAGATGTT CAATTTGCAG TCAAAAGCTG TGAAAAGAAA AGCTCCTGTA
    GAAAACATAA GCACACAAAA TTCTAGGGAT TCAGAAGCTA TCAGAAAAAA GACAAATGAT
    TCATTTTTGT ACACTTACAA ATCTGATGGG CCAGCCCATT GTAAAATGGC AGAGTCATCT
    TTGCAAAATG AAGACAGTGC TTGCTCACAG TCAAGGAACC TAGAACAAGA GACAGCTGGA
    GCATCAGAAT TGGGAGAAAA TAAGAAACAT AAAAAATCTT GCTTGGAACA TAACTCTTCA
    GAAAATCCAT GTGGAACCAC TTCAGAAATG TTTGGAAGCC CTTTTCAGAC ATTATATCAC
    TTTGAGGGGA GTGGAGAAGA TCTAGAAATA CAGAAAGTAA GTACTACTGT TAATAGCATG
    GCTGCCAACA TCCTGGAAAA TAACAGAATT CAGAATCAAC CAGAGAGATT TAAAGATGCT
    ACTGAAATGG ATTGCCAACC TCTGTCTTTT GAGACAGCAT TATCAGGAGA ACCTAGTGCT
    CAGAGAGAGG AAGAGAAAAG AAAAAAACCT AGTACTTGTG GAAGAATAAA TGTTTTTCGT
    TATGGACAAG TTAAATTATG TTCCACTGGC TTCATAACTC ATGTGGTACA GAATGAGCAA
    ACTAAATTAA CTGAAACAGA ACATTTATTT AAAAGTTATG CTCAACCTGG TCCTGTGAGT
    GCCAAAGAAA CATTTAGAAA TAGAACACAC CATTCAGTTG AGACTCCCAA CAGCAAAGAT
    TTAACCAGCA CTTTAAGTAA AGAATTTGCT GAACTACCCA ACAAAAGTCT GTACAGCACA
    AATCGAAGTT ATGAGCTAGA GAACAAACAT ACAGCAGCCT GTAAAAATTT TGCTATTTTT
    CAGGAAAGTG GTAAAAAATC ACACACAGGT GGCTTATTAC CTAACACATC CTCTTTTTCT
    TGGGGTGGAC ATGTTTCAGA TGGTAGTAAG AAAACAGACA AGTTGATTGG TCCTGCCGAA
    CCCATACCTC ATAAGAAGCT AAGCTTAAGC TCACAACTAG GATCTTTAGA GAAGTTTAAG
    AGGCAATATG GGAAGGTTAA AAATCCTCTG AATACTGAAG TGGAGGAAAA TAATACTTTA
    GAAATCACTA CCAATCTCAG TCCTCAGGTT GAAGCTGACA TTCCATGGAA AGACAAAAAC
    CACTTAGACA ACTCTGACAT CTGTGAAATC ACTACTATGA AATATAATGA TTCCTATGGT
    AGTTGTCAAC CAGTAAATCA CATGTTTTCT TCAGAAAAGC TTCCATTCTC CAAGGAAGAT
    GATTGTTTGG AACAACAGAT GCTTTGCTTA ACAGAAAGCA CTGTAACTCT ACAGGAGTTA
    CCTCATTTTA ACAGTAAATT TTTGGATGTT GAGAAGTCAC CTGAATCACT AGCCTCTAAA
    TTATCCAGAT TGAAGGGTTC CGAAAGAGAG ACTCAAAGGA TGGATGTGAA TCATTTTAAT
    GAATTGCTAC AATTAGATTC CAGTAGGAAA GATGGTGACT TGTCCAGTGG GTTAACCCTA
    GATTCTTGTA AGTTATTTAA AAATGAGCAT AAAAACCCAG AAAGTGACAG TATCCCAATG
    TCAGACTCTG TCACACAAGG TAATCTCTTC AGTAAAGACA GTGAAACATA TTCTAACAAC
    AATACCACTG AGAAGATACC AGAAAATCCT TTGGTACTGC CCTATGACCA TGCTACAGTT
    ATCAGTAAGG ATTCAGATAT TCTTATAGCT TCAAAACAAC AGATTGGAAG TCCTGACTCT
    CTGAGTAGAA TGTTAATGAG TCGCGTGGAA GATTCCACAG CTGACCAAAA TGGAACTTGT
    TTTCAGAGTG AGGAATCCAT AGCAAGAACT TGTTCTGAAA ATGAAGAGTC CAACATATGT
    TCTTTGGATT GGCAGCAGCA TTTTGATGTA ACTCTGGGAA GAATGGTTTA CATCAACAAA
    CTAACAGGAC TTAGCACATT CACTGCTCCT ACTGAGGATG TTCAGGCTGC TTGTACTAAA
    GACCTGACAA CTATGGCTGT GGATGTTCTA CTGGAGAATG CTACTGGGGA CGATGCCTTT
    GGTCCTGAAT CACTTCAATC TTTGTTCTCA GAATGGGACA ATCCAGTATT TGCCCGTTAT
    CCAGAGGTTG CTGTTGACGT AAGCAGCGGC CAAGCTGAGA GCCTAGCAGT TAAAATTCAC
    AACATCTTGT ATCCTTATCG TTTCACCAAA GAAATGATTC ACTCAATGCA GGTTCTCCAG
    CAAGTGGATA ACAAGTTTAT TGCCTGTTTA ATGAGCACTA AGACTGAAGA GAATGGTGAG
    GCAGGTGGAA ACCTGCTAGT CTTGGTGGAC CAGCACGCTG CCCACGAGCG CATCCGATTG
    GAGCAGCTGA TCATTGATTC CTATGAAAAG CAACAGCCAC AAGGCTTTGG TCGAAAAAAA
    TTACTGTCTT CCATTGTAAG TCCTCCACTA GAGATAACAG TGACAGAAGA ACAAAGGAGA
    CTCTTACGGT GTTACCACAA AAATCTGGAA GATCTGGGCC TTGAAATTGT ATTTCCAGAC
    ACCAGTGATT CACTGGTCCT CATAGGAAAA GTACCACTCT GTTTTGTAGA AAGAGAAGCA
    AGTGAACTTC GAAGAGGAAG ATCTACTGTG ACCAAGGGCA TTGTGGAGGA GTTTATTCGA
    GAACAAGTGG AGCTACTCCA GACCACAGGA GGCATCCAAG GGACTTTGCC ACTGACTGTC
    CAGAAGGTGT TGGCATCCCA AGCCTGCCAT GGGGCCATTA AGTTTAACGA TGGCCTGAGC
    TTCGAGGAGA GCTGCCGCCT TATTGAAGCG CTGTCCTGGT GCCAGCTGCC GTTCCAGTGT
    GCTCATGGCA GGCCCTCTAT GCTGCCATTA GCTAACATCG ACCACTTGGA GCAGGAAAAA
    CAGACTAAAC CCAATCTTGC TAAACTTCGC AGAATGGCTC AAGCCTGGCA TCTCTTTGGA
    AAGGAGAAGG ATGTGATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853911.1
    CDS214..4434
    Translation

    Target ORF information:

    RefSeq Version XM_024998143.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998143.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGATCAAAT GCTTGTCAGC TGAAGTACAA GCTAGATTGC GTTCTGGTTT GGCTGTATGC 
    TCCTTAGGCC AATGTGTTGA GGAGCTTGCC CTGAACAGTA TTGATGCTGA AGCAAAGTGT
    GTGGCTGTCA GGGTGAACAT GGAAACCTTT CAAGTTCAAG TGATAGACAA TGGATTTGGG
    ATGGGGAGTG ATGATGTAGA CAAGGTGGGA AATCGTTATT TCACCAGTAA ATGCAACTCC
    ATACAGGACT TGGAGAACCC AAGGTTTTAT GGTTTCCGGG GGGAGGCGTT GGCCAGTATA
    GCTGATATGG CCAGTGCTGT GGAAATTTCA TCCAAGAAAA ACAAGTCGAT GAAAACATTT
    GTGAAACTGT TTCAGAATGG GAAAGCCCTG AAAGCTTGTG AAGCTGACTT AACTCGACCA
    AGTGCCGGGA CGACAGTCAC TGTATATAAT CTGTTTTATC AGTTACCTGT TAGGAGGAAA
    TGCATGGATC CTAGACTGGA GTTTGAGAAG GTTAGGCAGA GGATAGAAGC TCTGTCACTC
    ATGCACCCTA CCATTTCTTT CTCTTTGAGA AATGATGTTT CTGGTTCCAT GGTTCTTCAA
    CTCCCTAAAA CCAAAGACAT ATGTTCCCGA TTTTGTCAAA TTTATGGACT GGGTAAGTCC
    CAAAAGTTAA GAGAAATAAA TTTTAAATAT AAGGAGTTTG AGCTTACTGG CTTTATCAGC
    TCCGAAGCAC ATTATAATAA GAATATGCAG TTTTTGTTTG TGAACAAGAG GTTAGTTTTA
    AGGACAAAGT TGCATAAACT CATTGACTTT TTATTAAGGA AAGAAAGTAT TATATGCAAA
    CCAAAGAGTG GTTCTGCCAG TAGGCAAGTG AATTCAAATC CTCGGCCTCG GTCTAACCCG
    GAACTCCATG GTATCTATGT AATCAATGTG CAGTGCCAGT TCTGTGAGTA TGACGTATGC
    CTGGAGCCAG CAAAAACCCT GATTGAGTTT CAGAACTGGG ATACTCTTTT GATTTGCATT
    CAGGAAGGAG TAAAAACGTT TTTAAAGAAA GAAAAATTAT TTGTGGAATT ATCAAGTGAA
    GACATTAAGG AATTTAGTGA AGATAATGAT TTTAGCTTGT TTAGTGCCAG TCTTCAGAAG
    CAAGTGTCCT CTGATGAGAA GTGTGGGCAG GTCAGTTTCC AAGAAGCATG TAATAATATT
    TTGGATTCCT ACGAGATGTT CAATTTGCAG TCAAAAGCTG TGAAAAGAAA AGCTCCTGTA
    GAAAACATAA GCACACAAAA TTCTAGGGAT TCAGAAGCTA TCAGAAAAAA GACAAATGAT
    TCATTTTTGT ACACTTACAA ATCTGATGGG CCAGCCCATT GTAAAATGGC AGAGTCATCT
    TTGCAAAATG AAGACAGTGC TTGCTCACAG TCAAGGAACC TAGAACAAGA GACAGCTGGA
    GCATCAGAAT TGGGAGAAAA TAAGAAACAT AAAAAATCTT GCTTGGAACA TAACTCTTCA
    GAAAATCCAT GTGGAACCAC TTCAGAAATG TTTGGAAGCC CTTTTCAGAC ATTATATCAC
    TTTGAGGGGA GTGGAGAAGA TCTAGAAATA CAGAAAGTAA GTACTACTGT TAATAGCATG
    GCTGCCAACA TCCTGGAAAA TAACAGAATT CAGAATCAAC CAGAGAGATT TAAAGATGCT
    ACTGAAATGG ATTGCCAACC TCTGTCTTTT GAGACAGCAT TATCAGGAGA ACCTAGTGCT
    CAGAGAGAGG AAGAGAAAAG AAAAAAACCT AGTACTTGTG GAAGAATAAA TGTTTTTCGT
    TATGGACAAG TTAAATTATG TTCCACTGGC TTCATAACTC ATGTGGTACA GAATGAGCAA
    ACTAAATTAA CTGAAACAGA ACATTTATTT AAAAGTTATG CTCAACCTGG TCCTGTGAGT
    GCCAAAGAAA CATTTAGAAA TAGAACACAC CATTCAGTTG AGACTCCCAA CAGCAAAGAT
    TTAACCAGCA CTTTAAGTAA AGAATTTGCT GAACTACCCA ACAAAAGTCT GTACAGCACA
    AATCGAAGTT ATGAGCTAGA GAACAAACAT ACAGCAGCCT GTAAAAATTT TGCTATTTTT
    CAGGAAAGTG GTAAAAAATC ACACACAGGT GGCTTATTAC CTAACACATC CTCTTTTTCT
    TGGGGTGGAC ATGTTTCAGA TGGTAGTAAG AAAACAGACA AGTTGATTGG TCCTGCCGAA
    CCCATACCTC ATAAGAAGCT AAGCTTAAGC TCACAACTAG GATCTTTAGA GAAGTTTAAG
    AGGCAATATG GGAAGGTTAA AAATCCTCTG AATACTGAAG TGGAGGAAAA TAATACTTTA
    GAAATCACTA CCAATCTCAG TCCTCAGGTT GAAGCTGACA TTCCATGGAA AGACAAAAAC
    CACTTAGACA ACTCTGACAT CTGTGAAATC ACTACTATGA AATATAATGA TTCCTATGGT
    AGTTGTCAAC CAGTAAATCA CATGTTTTCT TCAGAAAAGC TTCCATTCTC CAAGGAAGAT
    GATTGTTTGG AACAACAGAT GCTTTGCTTA ACAGAAAGCA CTGTAACTCT ACAGGAGTTA
    CCTCATTTTA ACAGTAAATT TTTGGATGTT GAGAAGTCAC CTGAATCACT AGCCTCTAAA
    TTATCCAGAT TGAAGGGTTC CGAAAGAGAG ACTCAAAGGA TGGATGTGAA TCATTTTAAT
    GAATTGCTAC AATTAGATTC CAGTAGGAAA GATGGTGACT TGTCCAGTGG GTTAACCCTA
    GATTCTTGTA AGTTATTTAA AAATGAGCAT AAAAACCCAG AAAGTGACAG TATCCCAATG
    TCAGACTCTG TCACACAAGG TAATCTCTTC AGTAAAGACA GTGAAACATA TTCTAACAAC
    AATACCACTG AGAAGATACC AGAAAATCCT TTGGTACTGC CCTATGACCA TGCTACAGTT
    ATCAGTAAGG ATTCAGATAT TCTTATAGCT TCAAAACAAC AGATTGGAAG TCCTGACTCT
    CTGAGTAGAA TGTTAATGAG TCGCGTGGAA GATTCCACAG CTGACCAAAA TGGAACTTGT
    TTTCAGAGTG AGGAATCCAT AGCAAGAACT TGTTCTGAAA ATGAAGAGTC CAACATATGT
    TCTTTGGATT GGCAGCAGCA TTTTGATGTA ACTCTGGGAA GAATGGTTTA CATCAACAAA
    CTAACAGGAC TTAGCACATT CACTGCTCCT ACTGAGGATG TTCAGGCTGC TTGTACTAAA
    GACCTGACAA CTATGGCTGT GGATGTTCTA CTGGAGAATG CTACTGGGGA CGATGCCTTT
    GGTCCTGAAT CACTTCAATC TTTGTTCTCA GAATGGGACA ATCCAGTATT TGCCCGTTAT
    CCAGAGGTTG CTGTTGACGT AAGCAGCGGC CAAGCTGAGA GCCTAGCAGT TAAAATTCAC
    AACATCTTGT ATCCTTATCG TTTCACCAAA GAAATGATTC ACTCAATGCA GGTTCTCCAG
    CAAGTGGATA ACAAGTTTAT TGCCTGTTTA ATGAGCACTA AGACTGAAGA GAATGGTGAG
    GCAGGTGGAA ACCTGCTAGT CTTGGTGGAC CAGCACGCTG CCCACGAGCG CATCCGATTG
    GAGCAGCTGA TCATTGATTC CTATGAAAAG CAACAGCCAC AAGGCTTTGG TCGAAAAAAA
    TTACTGTCTT CCATTGTAAG TCCTCCACTA GAGATAACAG TGACAGAAGA ACAAAGGAGA
    CTCTTACGGT GTTACCACAA AAATCTGGAA GATCTGGGCC TTGAAATTGT ATTTCCAGAC
    ACCAGTGATT CACTGGTCCT CATAGGAAAA GTACCACTCT GTTTTGTAGA AAGAGAAGCA
    AGTGAACTTC GAAGAGGAAG ATCTACTGTG ACCAAGGGCA TTGTGGAGGA GTTTATTCGA
    GAACAAGTGG AGCTACTCCA GACCACAGGA GGCATCCAAG GGACTTTGCC ACTGACTGTC
    CAGAAGGTGT TGGCATCCCA AGCCTGCCAT GGGGCCATTA AGTTTAACGA TGGCCTGAGC
    TTCGAGGAGA GCTGCCGCCT TATTGAAGCG CTGTCCTGGT GCCAGCTGCC GTTCCAGTGT
    GCTCATGGCA GGCCCTCTAT GCTGCCATTA GCTAACATCG ACCACTTGGA GCAGGAAAAA
    CAGACTAAAC CCAATCTTGC TAAACTTCGC AGAATGGCTC AAGCCTGGCA TCTCTTTGGA
    AAGGAGAAGG ATGTGATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57924
    Clone ID Related Accession (Same CDS sequence) XM_010809605.2 , XM_010809605.3 , XM_010813168.2
    Accession Version XM_010809605.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4464bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104283.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809605.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGTAA GTCCCAAAAG TTAAGAGAAA TAAATTTTAA ATATAAGGAG
    TTTGAGCTTA CTGGCTTTAT CAGCTCCGAA GCACATTATA ATAAGAATAT GCAGTTTTTG
    TTTGTGAACA AGAGGTTAGT TTTAAGGACA AAGTTGCATA AACTCATTGA CTTTTTATTA
    AGGAAAGAAA GTATTATATG CAAACCAAAG AGTGGTTCTG CCAGTAGGCA AGTGAATTCA
    AATCCTCGGC CTCGGTCTAA CCCGGAACTC CATGGTATCT ATGTAATCAA TGTGCAGTGC
    CAGTTCTGTG AGTATGACGT ATGCCTGGAG CCAGCAAAAA CCCTGATTGA GTTTCAGAAC
    TGGGATACTC TTTTGATTTG CATTCAGGAA GGAGTAAAAA CGTTTTTAAA GAAAGAAAAA
    TTATTTGTGG AATTATCAAG TGAAGACATT AAGGAATTTA GTGAAGATAA TGATTTTAGC
    TTGTTTAGTG CCAGTCTTCA GAAGCAAGTG TCCTCTGATG AGAAGTGTGG GCAGGTCAGT
    TTCCAAGAAG CATGTAATAA TATTTTGGAT TCCTACGAGA TGTTCAATTT GCAGTCAAAA
    GCTGTGAAAA GAAAAGCTCC TGTAGAAAAC ATAAGCACAC AAAATTCTAG GGATTCAGAA
    GCTATCAGAA AAAAGACAAA TGATTCATTT TTGTACACTT ACAAATCTGA TGGGCCAGCC
    CATTGTAAAA TGGCAGAGTC ATCTTTGCAA AATGAAGACA GTGCTTGCTC ACAGTCAAGG
    AACCTAGAAC AAGAGACAGC TGGAGCATCA GAATTGGGAG AAAATAAGAA ACATAAAAAA
    TCTTGCTTGG AACATAACTC TTCAGAAAAT CCATGTGGAA CCACTTCAGA AATGTTTGGA
    AGCCCTTTTC AGACATTATA TCACTTTGAG GGGAGTGGAG AAGATCTAGA AATACAGAAA
    GTAAGTACTA CTGTTAATAG CATGGCTGCC AACATCCTGG AAAATAACAG AATTCAGAAT
    CAACCAGAGA GATTTAAAGA TGCTACTGAA ATGGATTGCC AACCTCTGTC TTTTGAGACA
    GCATTATCAG GAGAACCTAG TGCTCAGAGA GAGGAAGAGA AAAGAAAAAA ACCTAGTACT
    TGTGGAAGAA TAAATGTTTT TCGTTATGGA CAAGTTAAAT TATGTTCCAC TGGCTTCATA
    ACTCATGTGG TACAGAATGA GCAAACTAAA TTAACTGAAA CAGAACATTT ATTTAAAAGT
    TATGCTCAAC CTGGTCCTGT GAGTGCCAAA GAAACATTTA GAAATAGAAC ACACCATTCA
    GTTGAGACTC CCAACAGCAA AGATTTAACC AGCACTTTAA GTAAAGAATT TGCTGAACTA
    CCCAACAAAA GTCTGTACAG CACAAATCGA AGTTATGAGC TAGAGAACAA ACATACAGCA
    GCCTGTAAAA ATTTTGCTAT TTTTCAGGAA AGTGGTAAAA AATCACACAC AGGTGGCTTA
    TTACCTAACA CATCCTCTTT TTCTTGGGGT GGACATGTTT CAGATGGTAG TAAGAAAACA
    GACAAGTTGA TTGGTCCTGC CGAACCCATA CCTCATAAGA AGCTAAGCTT AAGCTCACAA
    CTAGGATCTT TAGAGAAGTT TAAGAGGCAA TATGGGAAGG TTAAAAATCC TCTGAATACT
    GAAGTGGAGG AAAATAATAC TTTAGAAATC ACTACCAATC TCAGTCCTCA GGTTGAAGCT
    GACATTCCAT GGAAAGACAA AAACCACTTA GACAACTCTG ACATCTGTGA AATCACTACT
    ATGAAATATA ATGATTCCTA TGGTAGTTGT CAACCAGTAA ATCACATGTT TTCTTCAGAA
    AAGCTTCCAT TCTCCAAGGA AGATGATTGT TTGGAACAAC AGATGCTTTG CTTAACAGAA
    AGCACTGTAA CTCTACAGGA GTTACCTCAT TTTAACAGTA AATTTTTGGA TGTTGAGAAG
    TCACCTGAAT CACTAGCCTC TAAATTATCC AGATTGAAGG GTTCCGAAAG AGAGACTCAA
    AGGATGGATG TGAATCATTT TAATGAATTG CTACAATTAG ATTCCAGTAG GAAAGATGGT
    GACTTGTCCA GTGGGTTAAC CCTAGATTCT TGTAAGTTAT TTAAAAATGA GCATAAAAAC
    CCAGAAAGTG ACAGTATCCC AATGTCAGAC TCTGTCACAC AAGGTAATCT CTTCAGTAAA
    GACAGTGAAA CATATTCTAA CAACAATACC ACTGAGAAGA TACCAGAAAA TCCTTTGGTA
    CTGCCCTATG ACCATGCTAC AGTTATCAGT AAGGATTCAG ATATTCTTAT AGCTTCAAAA
    CAACAGATTG GAAGTCCTGA CTCTCTGAGT AGAATGTTAA TGAGTCGCGT GGAAGATTCC
    ACAGCTGACC AAAATGGAAC TTGTTTTCAG AGTGAGGAAT CCATAGCAAG AACTTGTTCT
    GAAAATGAAG AGTCCAACAT ATGTTCTTTG GATTGGCAGC AGCATTTTGA TGTAACTCTG
    GGAAGAATGG TTTACATCAA CAAACTAACA GGACTTAGCA CATTCACTGC TCCTACTGAG
    GATGTTCAGG CTGCTTGTAC TAAAGACCTG ACAACTATGG CTGTGGATGT TCTACTGGAG
    AATGGATCTC AGTACAGGTG TCATCCCTTT AGAAGCGACC TTGTTCTTCC TTTCCTTCCT
    AGAGCTCGGG AAGAGAGGAC TGTGATGAGA CAGAATAACA GAGCTACTGG GGACGATGCC
    TTTGGTCCTG AATCACTTCA ATCTTTGTTC TCAGAATGGG ACAATCCAGT ATTTGCCCGT
    TATCCAGAGG TTGCTGTTGA CGTAAGCAGC GGCCAAGCTG AGAGCCTAGC AGTTAAAATT
    CACAACATCT TGTATCCTTA TCGTTTCACC AAAGAAATGA TTCACTCAAT GCAGGTTCTC
    CAGCAAGTGG ATAACAAGTT TATTGCCTGT TTAATGAGCA CTAAGACTGA AGAGAATGGT
    GAGGCAGGTG GAAACCTGCT AGTCTTGGTG GACCAGCACG CTGCCCACGA GCGCATCCGA
    TTGGAGCAGC TGATCATTGA TTCCTATGAA AAGCAACAGC CACAAGGCTT TGGTCGAAAA
    AAATTACTGT CTTCCATTGT AAGTCCTCCA CTAGAGATAA CAGTGACAGA AGAACAAAGG
    AGACTCTTAC GGTGTTACCA CAAAAATCTG GAAGATCTGG GCCTTGAAAT TGTATTTCCA
    GACACCAGTG ATTCACTGGT CCTCATAGGA AAAGTACCAC TCTGTTTTGT AGAAAGAGAA
    GCAAGTGAAC TTCGAAGAGG AAGATCTACT GTGACCAAGG GCATTGTGGA GGAGTTTATT
    CGAGAACAAG TGGAGCTACT CCAGACCACA GGAGGCATCC AAGGGACTTT GCCACTGACT
    GTCCAGAAGG TGTTGGCATC CCAAGCCTGC CATGGGGCCA TTAAGTTTAA CGATGGCCTG
    AGCTTCGAGG AGAGCTGCCG CCTTATTGAA GCGCTGTCCT GGTGCCAGCT GCCGTTCCAG
    TGTGCTCATG GCAGGCCCTC TATGCTGCCA TTAGCTAACA TCGACCACTT GGAGCAGGAA
    AAACAGACTA AACCCAATCT TGCTAAACTT CGCAGAATGG CTCAAGCCTG GCATCTCTTT
    GGAAAGGAGA AGGATGTGAT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807907.1
    CDS9..4472
    Translation

    Target ORF information:

    RefSeq Version XM_010809605.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809605.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGTAA GTCCCAAAAG TTAAGAGAAA TAAATTTTAA ATATAAGGAG
    TTTGAGCTTA CTGGCTTTAT CAGCTCCGAA GCACATTATA ATAAGAATAT GCAGTTTTTG
    TTTGTGAACA AGAGGTTAGT TTTAAGGACA AAGTTGCATA AACTCATTGA CTTTTTATTA
    AGGAAAGAAA GTATTATATG CAAACCAAAG AGTGGTTCTG CCAGTAGGCA AGTGAATTCA
    AATCCTCGGC CTCGGTCTAA CCCGGAACTC CATGGTATCT ATGTAATCAA TGTGCAGTGC
    CAGTTCTGTG AGTATGACGT ATGCCTGGAG CCAGCAAAAA CCCTGATTGA GTTTCAGAAC
    TGGGATACTC TTTTGATTTG CATTCAGGAA GGAGTAAAAA CGTTTTTAAA GAAAGAAAAA
    TTATTTGTGG AATTATCAAG TGAAGACATT AAGGAATTTA GTGAAGATAA TGATTTTAGC
    TTGTTTAGTG CCAGTCTTCA GAAGCAAGTG TCCTCTGATG AGAAGTGTGG GCAGGTCAGT
    TTCCAAGAAG CATGTAATAA TATTTTGGAT TCCTACGAGA TGTTCAATTT GCAGTCAAAA
    GCTGTGAAAA GAAAAGCTCC TGTAGAAAAC ATAAGCACAC AAAATTCTAG GGATTCAGAA
    GCTATCAGAA AAAAGACAAA TGATTCATTT TTGTACACTT ACAAATCTGA TGGGCCAGCC
    CATTGTAAAA TGGCAGAGTC ATCTTTGCAA AATGAAGACA GTGCTTGCTC ACAGTCAAGG
    AACCTAGAAC AAGAGACAGC TGGAGCATCA GAATTGGGAG AAAATAAGAA ACATAAAAAA
    TCTTGCTTGG AACATAACTC TTCAGAAAAT CCATGTGGAA CCACTTCAGA AATGTTTGGA
    AGCCCTTTTC AGACATTATA TCACTTTGAG GGGAGTGGAG AAGATCTAGA AATACAGAAA
    GTAAGTACTA CTGTTAATAG CATGGCTGCC AACATCCTGG AAAATAACAG AATTCAGAAT
    CAACCAGAGA GATTTAAAGA TGCTACTGAA ATGGATTGCC AACCTCTGTC TTTTGAGACA
    GCATTATCAG GAGAACCTAG TGCTCAGAGA GAGGAAGAGA AAAGAAAAAA ACCTAGTACT
    TGTGGAAGAA TAAATGTTTT TCGTTATGGA CAAGTTAAAT TATGTTCCAC TGGCTTCATA
    ACTCATGTGG TACAGAATGA GCAAACTAAA TTAACTGAAA CAGAACATTT ATTTAAAAGT
    TATGCTCAAC CTGGTCCTGT GAGTGCCAAA GAAACATTTA GAAATAGAAC ACACCATTCA
    GTTGAGACTC CCAACAGCAA AGATTTAACC AGCACTTTAA GTAAAGAATT TGCTGAACTA
    CCCAACAAAA GTCTGTACAG CACAAATCGA AGTTATGAGC TAGAGAACAA ACATACAGCA
    GCCTGTAAAA ATTTTGCTAT TTTTCAGGAA AGTGGTAAAA AATCACACAC AGGTGGCTTA
    TTACCTAACA CATCCTCTTT TTCTTGGGGT GGACATGTTT CAGATGGTAG TAAGAAAACA
    GACAAGTTGA TTGGTCCTGC CGAACCCATA CCTCATAAGA AGCTAAGCTT AAGCTCACAA
    CTAGGATCTT TAGAGAAGTT TAAGAGGCAA TATGGGAAGG TTAAAAATCC TCTGAATACT
    GAAGTGGAGG AAAATAATAC TTTAGAAATC ACTACCAATC TCAGTCCTCA GGTTGAAGCT
    GACATTCCAT GGAAAGACAA AAACCACTTA GACAACTCTG ACATCTGTGA AATCACTACT
    ATGAAATATA ATGATTCCTA TGGTAGTTGT CAACCAGTAA ATCACATGTT TTCTTCAGAA
    AAGCTTCCAT TCTCCAAGGA AGATGATTGT TTGGAACAAC AGATGCTTTG CTTAACAGAA
    AGCACTGTAA CTCTACAGGA GTTACCTCAT TTTAACAGTA AATTTTTGGA TGTTGAGAAG
    TCACCTGAAT CACTAGCCTC TAAATTATCC AGATTGAAGG GTTCCGAAAG AGAGACTCAA
    AGGATGGATG TGAATCATTT TAATGAATTG CTACAATTAG ATTCCAGTAG GAAAGATGGT
    GACTTGTCCA GTGGGTTAAC CCTAGATTCT TGTAAGTTAT TTAAAAATGA GCATAAAAAC
    CCAGAAAGTG ACAGTATCCC AATGTCAGAC TCTGTCACAC AAGGTAATCT CTTCAGTAAA
    GACAGTGAAA CATATTCTAA CAACAATACC ACTGAGAAGA TACCAGAAAA TCCTTTGGTA
    CTGCCCTATG ACCATGCTAC AGTTATCAGT AAGGATTCAG ATATTCTTAT AGCTTCAAAA
    CAACAGATTG GAAGTCCTGA CTCTCTGAGT AGAATGTTAA TGAGTCGCGT GGAAGATTCC
    ACAGCTGACC AAAATGGAAC TTGTTTTCAG AGTGAGGAAT CCATAGCAAG AACTTGTTCT
    GAAAATGAAG AGTCCAACAT ATGTTCTTTG GATTGGCAGC AGCATTTTGA TGTAACTCTG
    GGAAGAATGG TTTACATCAA CAAACTAACA GGACTTAGCA CATTCACTGC TCCTACTGAG
    GATGTTCAGG CTGCTTGTAC TAAAGACCTG ACAACTATGG CTGTGGATGT TCTACTGGAG
    AATGGATCTC AGTACAGGTG TCATCCCTTT AGAAGCGACC TTGTTCTTCC TTTCCTTCCT
    AGAGCTCGGG AAGAGAGGAC TGTGATGAGA CAGAATAACA GAGCTACTGG GGACGATGCC
    TTTGGTCCTG AATCACTTCA ATCTTTGTTC TCAGAATGGG ACAATCCAGT ATTTGCCCGT
    TATCCAGAGG TTGCTGTTGA CGTAAGCAGC GGCCAAGCTG AGAGCCTAGC AGTTAAAATT
    CACAACATCT TGTATCCTTA TCGTTTCACC AAAGAAATGA TTCACTCAAT GCAGGTTCTC
    CAGCAAGTGG ATAACAAGTT TATTGCCTGT TTAATGAGCA CTAAGACTGA AGAGAATGGT
    GAGGCAGGTG GAAACCTGCT AGTCTTGGTG GACCAGCACG CTGCCCACGA GCGCATCCGA
    TTGGAGCAGC TGATCATTGA TTCCTATGAA AAGCAACAGC CACAAGGCTT TGGTCGAAAA
    AAATTACTGT CTTCCATTGT AAGTCCTCCA CTAGAGATAA CAGTGACAGA AGAACAAAGG
    AGACTCTTAC GGTGTTACCA CAAAAATCTG GAAGATCTGG GCCTTGAAAT TGTATTTCCA
    GACACCAGTG ATTCACTGGT CCTCATAGGA AAAGTACCAC TCTGTTTTGT AGAAAGAGAA
    GCAAGTGAAC TTCGAAGAGG AAGATCTACT GTGACCAAGG GCATTGTGGA GGAGTTTATT
    CGAGAACAAG TGGAGCTACT CCAGACCACA GGAGGCATCC AAGGGACTTT GCCACTGACT
    GTCCAGAAGG TGTTGGCATC CCAAGCCTGC CATGGGGCCA TTAAGTTTAA CGATGGCCTG
    AGCTTCGAGG AGAGCTGCCG CCTTATTGAA GCGCTGTCCT GGTGCCAGCT GCCGTTCCAG
    TGTGCTCATG GCAGGCCCTC TATGCTGCCA TTAGCTAACA TCGACCACTT GGAGCAGGAA
    AAACAGACTA AACCCAATCT TGCTAAACTT CGCAGAATGG CTCAAGCCTG GCATCTCTTT
    GGAAAGGAGA AGGATGTGAT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57924
    Clone ID Related Accession (Same CDS sequence) XM_010809605.2 , XM_010809605.3 , XM_010813168.2
    Accession Version XM_010809605.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4464bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809605.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGTAA GTCCCAAAAG TTAAGAGAAA TAAATTTTAA ATATAAGGAG
    TTTGAGCTTA CTGGCTTTAT CAGCTCCGAA GCACATTATA ATAAGAATAT GCAGTTTTTG
    TTTGTGAACA AGAGGTTAGT TTTAAGGACA AAGTTGCATA AACTCATTGA CTTTTTATTA
    AGGAAAGAAA GTATTATATG CAAACCAAAG AGTGGTTCTG CCAGTAGGCA AGTGAATTCA
    AATCCTCGGC CTCGGTCTAA CCCGGAACTC CATGGTATCT ATGTAATCAA TGTGCAGTGC
    CAGTTCTGTG AGTATGACGT ATGCCTGGAG CCAGCAAAAA CCCTGATTGA GTTTCAGAAC
    TGGGATACTC TTTTGATTTG CATTCAGGAA GGAGTAAAAA CGTTTTTAAA GAAAGAAAAA
    TTATTTGTGG AATTATCAAG TGAAGACATT AAGGAATTTA GTGAAGATAA TGATTTTAGC
    TTGTTTAGTG CCAGTCTTCA GAAGCAAGTG TCCTCTGATG AGAAGTGTGG GCAGGTCAGT
    TTCCAAGAAG CATGTAATAA TATTTTGGAT TCCTACGAGA TGTTCAATTT GCAGTCAAAA
    GCTGTGAAAA GAAAAGCTCC TGTAGAAAAC ATAAGCACAC AAAATTCTAG GGATTCAGAA
    GCTATCAGAA AAAAGACAAA TGATTCATTT TTGTACACTT ACAAATCTGA TGGGCCAGCC
    CATTGTAAAA TGGCAGAGTC ATCTTTGCAA AATGAAGACA GTGCTTGCTC ACAGTCAAGG
    AACCTAGAAC AAGAGACAGC TGGAGCATCA GAATTGGGAG AAAATAAGAA ACATAAAAAA
    TCTTGCTTGG AACATAACTC TTCAGAAAAT CCATGTGGAA CCACTTCAGA AATGTTTGGA
    AGCCCTTTTC AGACATTATA TCACTTTGAG GGGAGTGGAG AAGATCTAGA AATACAGAAA
    GTAAGTACTA CTGTTAATAG CATGGCTGCC AACATCCTGG AAAATAACAG AATTCAGAAT
    CAACCAGAGA GATTTAAAGA TGCTACTGAA ATGGATTGCC AACCTCTGTC TTTTGAGACA
    GCATTATCAG GAGAACCTAG TGCTCAGAGA GAGGAAGAGA AAAGAAAAAA ACCTAGTACT
    TGTGGAAGAA TAAATGTTTT TCGTTATGGA CAAGTTAAAT TATGTTCCAC TGGCTTCATA
    ACTCATGTGG TACAGAATGA GCAAACTAAA TTAACTGAAA CAGAACATTT ATTTAAAAGT
    TATGCTCAAC CTGGTCCTGT GAGTGCCAAA GAAACATTTA GAAATAGAAC ACACCATTCA
    GTTGAGACTC CCAACAGCAA AGATTTAACC AGCACTTTAA GTAAAGAATT TGCTGAACTA
    CCCAACAAAA GTCTGTACAG CACAAATCGA AGTTATGAGC TAGAGAACAA ACATACAGCA
    GCCTGTAAAA ATTTTGCTAT TTTTCAGGAA AGTGGTAAAA AATCACACAC AGGTGGCTTA
    TTACCTAACA CATCCTCTTT TTCTTGGGGT GGACATGTTT CAGATGGTAG TAAGAAAACA
    GACAAGTTGA TTGGTCCTGC CGAACCCATA CCTCATAAGA AGCTAAGCTT AAGCTCACAA
    CTAGGATCTT TAGAGAAGTT TAAGAGGCAA TATGGGAAGG TTAAAAATCC TCTGAATACT
    GAAGTGGAGG AAAATAATAC TTTAGAAATC ACTACCAATC TCAGTCCTCA GGTTGAAGCT
    GACATTCCAT GGAAAGACAA AAACCACTTA GACAACTCTG ACATCTGTGA AATCACTACT
    ATGAAATATA ATGATTCCTA TGGTAGTTGT CAACCAGTAA ATCACATGTT TTCTTCAGAA
    AAGCTTCCAT TCTCCAAGGA AGATGATTGT TTGGAACAAC AGATGCTTTG CTTAACAGAA
    AGCACTGTAA CTCTACAGGA GTTACCTCAT TTTAACAGTA AATTTTTGGA TGTTGAGAAG
    TCACCTGAAT CACTAGCCTC TAAATTATCC AGATTGAAGG GTTCCGAAAG AGAGACTCAA
    AGGATGGATG TGAATCATTT TAATGAATTG CTACAATTAG ATTCCAGTAG GAAAGATGGT
    GACTTGTCCA GTGGGTTAAC CCTAGATTCT TGTAAGTTAT TTAAAAATGA GCATAAAAAC
    CCAGAAAGTG ACAGTATCCC AATGTCAGAC TCTGTCACAC AAGGTAATCT CTTCAGTAAA
    GACAGTGAAA CATATTCTAA CAACAATACC ACTGAGAAGA TACCAGAAAA TCCTTTGGTA
    CTGCCCTATG ACCATGCTAC AGTTATCAGT AAGGATTCAG ATATTCTTAT AGCTTCAAAA
    CAACAGATTG GAAGTCCTGA CTCTCTGAGT AGAATGTTAA TGAGTCGCGT GGAAGATTCC
    ACAGCTGACC AAAATGGAAC TTGTTTTCAG AGTGAGGAAT CCATAGCAAG AACTTGTTCT
    GAAAATGAAG AGTCCAACAT ATGTTCTTTG GATTGGCAGC AGCATTTTGA TGTAACTCTG
    GGAAGAATGG TTTACATCAA CAAACTAACA GGACTTAGCA CATTCACTGC TCCTACTGAG
    GATGTTCAGG CTGCTTGTAC TAAAGACCTG ACAACTATGG CTGTGGATGT TCTACTGGAG
    AATGGATCTC AGTACAGGTG TCATCCCTTT AGAAGCGACC TTGTTCTTCC TTTCCTTCCT
    AGAGCTCGGG AAGAGAGGAC TGTGATGAGA CAGAATAACA GAGCTACTGG GGACGATGCC
    TTTGGTCCTG AATCACTTCA ATCTTTGTTC TCAGAATGGG ACAATCCAGT ATTTGCCCGT
    TATCCAGAGG TTGCTGTTGA CGTAAGCAGC GGCCAAGCTG AGAGCCTAGC AGTTAAAATT
    CACAACATCT TGTATCCTTA TCGTTTCACC AAAGAAATGA TTCACTCAAT GCAGGTTCTC
    CAGCAAGTGG ATAACAAGTT TATTGCCTGT TTAATGAGCA CTAAGACTGA AGAGAATGGT
    GAGGCAGGTG GAAACCTGCT AGTCTTGGTG GACCAGCACG CTGCCCACGA GCGCATCCGA
    TTGGAGCAGC TGATCATTGA TTCCTATGAA AAGCAACAGC CACAAGGCTT TGGTCGAAAA
    AAATTACTGT CTTCCATTGT AAGTCCTCCA CTAGAGATAA CAGTGACAGA AGAACAAAGG
    AGACTCTTAC GGTGTTACCA CAAAAATCTG GAAGATCTGG GCCTTGAAAT TGTATTTCCA
    GACACCAGTG ATTCACTGGT CCTCATAGGA AAAGTACCAC TCTGTTTTGT AGAAAGAGAA
    GCAAGTGAAC TTCGAAGAGG AAGATCTACT GTGACCAAGG GCATTGTGGA GGAGTTTATT
    CGAGAACAAG TGGAGCTACT CCAGACCACA GGAGGCATCC AAGGGACTTT GCCACTGACT
    GTCCAGAAGG TGTTGGCATC CCAAGCCTGC CATGGGGCCA TTAAGTTTAA CGATGGCCTG
    AGCTTCGAGG AGAGCTGCCG CCTTATTGAA GCGCTGTCCT GGTGCCAGCT GCCGTTCCAG
    TGTGCTCATG GCAGGCCCTC TATGCTGCCA TTAGCTAACA TCGACCACTT GGAGCAGGAA
    AAACAGACTA AACCCAATCT TGCTAAACTT CGCAGAATGG CTCAAGCCTG GCATCTCTTT
    GGAAAGGAGA AGGATGTGAT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807907.1
    CDS61..4524
    Translation

    Target ORF information:

    RefSeq Version XM_010809605.3
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809605.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGTAA GTCCCAAAAG TTAAGAGAAA TAAATTTTAA ATATAAGGAG
    TTTGAGCTTA CTGGCTTTAT CAGCTCCGAA GCACATTATA ATAAGAATAT GCAGTTTTTG
    TTTGTGAACA AGAGGTTAGT TTTAAGGACA AAGTTGCATA AACTCATTGA CTTTTTATTA
    AGGAAAGAAA GTATTATATG CAAACCAAAG AGTGGTTCTG CCAGTAGGCA AGTGAATTCA
    AATCCTCGGC CTCGGTCTAA CCCGGAACTC CATGGTATCT ATGTAATCAA TGTGCAGTGC
    CAGTTCTGTG AGTATGACGT ATGCCTGGAG CCAGCAAAAA CCCTGATTGA GTTTCAGAAC
    TGGGATACTC TTTTGATTTG CATTCAGGAA GGAGTAAAAA CGTTTTTAAA GAAAGAAAAA
    TTATTTGTGG AATTATCAAG TGAAGACATT AAGGAATTTA GTGAAGATAA TGATTTTAGC
    TTGTTTAGTG CCAGTCTTCA GAAGCAAGTG TCCTCTGATG AGAAGTGTGG GCAGGTCAGT
    TTCCAAGAAG CATGTAATAA TATTTTGGAT TCCTACGAGA TGTTCAATTT GCAGTCAAAA
    GCTGTGAAAA GAAAAGCTCC TGTAGAAAAC ATAAGCACAC AAAATTCTAG GGATTCAGAA
    GCTATCAGAA AAAAGACAAA TGATTCATTT TTGTACACTT ACAAATCTGA TGGGCCAGCC
    CATTGTAAAA TGGCAGAGTC ATCTTTGCAA AATGAAGACA GTGCTTGCTC ACAGTCAAGG
    AACCTAGAAC AAGAGACAGC TGGAGCATCA GAATTGGGAG AAAATAAGAA ACATAAAAAA
    TCTTGCTTGG AACATAACTC TTCAGAAAAT CCATGTGGAA CCACTTCAGA AATGTTTGGA
    AGCCCTTTTC AGACATTATA TCACTTTGAG GGGAGTGGAG AAGATCTAGA AATACAGAAA
    GTAAGTACTA CTGTTAATAG CATGGCTGCC AACATCCTGG AAAATAACAG AATTCAGAAT
    CAACCAGAGA GATTTAAAGA TGCTACTGAA ATGGATTGCC AACCTCTGTC TTTTGAGACA
    GCATTATCAG GAGAACCTAG TGCTCAGAGA GAGGAAGAGA AAAGAAAAAA ACCTAGTACT
    TGTGGAAGAA TAAATGTTTT TCGTTATGGA CAAGTTAAAT TATGTTCCAC TGGCTTCATA
    ACTCATGTGG TACAGAATGA GCAAACTAAA TTAACTGAAA CAGAACATTT ATTTAAAAGT
    TATGCTCAAC CTGGTCCTGT GAGTGCCAAA GAAACATTTA GAAATAGAAC ACACCATTCA
    GTTGAGACTC CCAACAGCAA AGATTTAACC AGCACTTTAA GTAAAGAATT TGCTGAACTA
    CCCAACAAAA GTCTGTACAG CACAAATCGA AGTTATGAGC TAGAGAACAA ACATACAGCA
    GCCTGTAAAA ATTTTGCTAT TTTTCAGGAA AGTGGTAAAA AATCACACAC AGGTGGCTTA
    TTACCTAACA CATCCTCTTT TTCTTGGGGT GGACATGTTT CAGATGGTAG TAAGAAAACA
    GACAAGTTGA TTGGTCCTGC CGAACCCATA CCTCATAAGA AGCTAAGCTT AAGCTCACAA
    CTAGGATCTT TAGAGAAGTT TAAGAGGCAA TATGGGAAGG TTAAAAATCC TCTGAATACT
    GAAGTGGAGG AAAATAATAC TTTAGAAATC ACTACCAATC TCAGTCCTCA GGTTGAAGCT
    GACATTCCAT GGAAAGACAA AAACCACTTA GACAACTCTG ACATCTGTGA AATCACTACT
    ATGAAATATA ATGATTCCTA TGGTAGTTGT CAACCAGTAA ATCACATGTT TTCTTCAGAA
    AAGCTTCCAT TCTCCAAGGA AGATGATTGT TTGGAACAAC AGATGCTTTG CTTAACAGAA
    AGCACTGTAA CTCTACAGGA GTTACCTCAT TTTAACAGTA AATTTTTGGA TGTTGAGAAG
    TCACCTGAAT CACTAGCCTC TAAATTATCC AGATTGAAGG GTTCCGAAAG AGAGACTCAA
    AGGATGGATG TGAATCATTT TAATGAATTG CTACAATTAG ATTCCAGTAG GAAAGATGGT
    GACTTGTCCA GTGGGTTAAC CCTAGATTCT TGTAAGTTAT TTAAAAATGA GCATAAAAAC
    CCAGAAAGTG ACAGTATCCC AATGTCAGAC TCTGTCACAC AAGGTAATCT CTTCAGTAAA
    GACAGTGAAA CATATTCTAA CAACAATACC ACTGAGAAGA TACCAGAAAA TCCTTTGGTA
    CTGCCCTATG ACCATGCTAC AGTTATCAGT AAGGATTCAG ATATTCTTAT AGCTTCAAAA
    CAACAGATTG GAAGTCCTGA CTCTCTGAGT AGAATGTTAA TGAGTCGCGT GGAAGATTCC
    ACAGCTGACC AAAATGGAAC TTGTTTTCAG AGTGAGGAAT CCATAGCAAG AACTTGTTCT
    GAAAATGAAG AGTCCAACAT ATGTTCTTTG GATTGGCAGC AGCATTTTGA TGTAACTCTG
    GGAAGAATGG TTTACATCAA CAAACTAACA GGACTTAGCA CATTCACTGC TCCTACTGAG
    GATGTTCAGG CTGCTTGTAC TAAAGACCTG ACAACTATGG CTGTGGATGT TCTACTGGAG
    AATGGATCTC AGTACAGGTG TCATCCCTTT AGAAGCGACC TTGTTCTTCC TTTCCTTCCT
    AGAGCTCGGG AAGAGAGGAC TGTGATGAGA CAGAATAACA GAGCTACTGG GGACGATGCC
    TTTGGTCCTG AATCACTTCA ATCTTTGTTC TCAGAATGGG ACAATCCAGT ATTTGCCCGT
    TATCCAGAGG TTGCTGTTGA CGTAAGCAGC GGCCAAGCTG AGAGCCTAGC AGTTAAAATT
    CACAACATCT TGTATCCTTA TCGTTTCACC AAAGAAATGA TTCACTCAAT GCAGGTTCTC
    CAGCAAGTGG ATAACAAGTT TATTGCCTGT TTAATGAGCA CTAAGACTGA AGAGAATGGT
    GAGGCAGGTG GAAACCTGCT AGTCTTGGTG GACCAGCACG CTGCCCACGA GCGCATCCGA
    TTGGAGCAGC TGATCATTGA TTCCTATGAA AAGCAACAGC CACAAGGCTT TGGTCGAAAA
    AAATTACTGT CTTCCATTGT AAGTCCTCCA CTAGAGATAA CAGTGACAGA AGAACAAAGG
    AGACTCTTAC GGTGTTACCA CAAAAATCTG GAAGATCTGG GCCTTGAAAT TGTATTTCCA
    GACACCAGTG ATTCACTGGT CCTCATAGGA AAAGTACCAC TCTGTTTTGT AGAAAGAGAA
    GCAAGTGAAC TTCGAAGAGG AAGATCTACT GTGACCAAGG GCATTGTGGA GGAGTTTATT
    CGAGAACAAG TGGAGCTACT CCAGACCACA GGAGGCATCC AAGGGACTTT GCCACTGACT
    GTCCAGAAGG TGTTGGCATC CCAAGCCTGC CATGGGGCCA TTAAGTTTAA CGATGGCCTG
    AGCTTCGAGG AGAGCTGCCG CCTTATTGAA GCGCTGTCCT GGTGCCAGCT GCCGTTCCAG
    TGTGCTCATG GCAGGCCCTC TATGCTGCCA TTAGCTAACA TCGACCACTT GGAGCAGGAA
    AAACAGACTA AACCCAATCT TGCTAAACTT CGCAGAATGG CTCAAGCCTG GCATCTCTTT
    GGAAAGGAGA AGGATGTGAT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57924
    Clone ID Related Accession (Same CDS sequence) XM_010809605.2 , XM_010809605.3 , XM_010813168.2
    Accession Version XM_010813168.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4464bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813168.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGTAA GTCCCAAAAG TTAAGAGAAA TAAATTTTAA ATATAAGGAG
    TTTGAGCTTA CTGGCTTTAT CAGCTCCGAA GCACATTATA ATAAGAATAT GCAGTTTTTG
    TTTGTGAACA AGAGGTTAGT TTTAAGGACA AAGTTGCATA AACTCATTGA CTTTTTATTA
    AGGAAAGAAA GTATTATATG CAAACCAAAG AGTGGTTCTG CCAGTAGGCA AGTGAATTCA
    AATCCTCGGC CTCGGTCTAA CCCGGAACTC CATGGTATCT ATGTAATCAA TGTGCAGTGC
    CAGTTCTGTG AGTATGACGT ATGCCTGGAG CCAGCAAAAA CCCTGATTGA GTTTCAGAAC
    TGGGATACTC TTTTGATTTG CATTCAGGAA GGAGTAAAAA CGTTTTTAAA GAAAGAAAAA
    TTATTTGTGG AATTATCAAG TGAAGACATT AAGGAATTTA GTGAAGATAA TGATTTTAGC
    TTGTTTAGTG CCAGTCTTCA GAAGCAAGTG TCCTCTGATG AGAAGTGTGG GCAGGTCAGT
    TTCCAAGAAG CATGTAATAA TATTTTGGAT TCCTACGAGA TGTTCAATTT GCAGTCAAAA
    GCTGTGAAAA GAAAAGCTCC TGTAGAAAAC ATAAGCACAC AAAATTCTAG GGATTCAGAA
    GCTATCAGAA AAAAGACAAA TGATTCATTT TTGTACACTT ACAAATCTGA TGGGCCAGCC
    CATTGTAAAA TGGCAGAGTC ATCTTTGCAA AATGAAGACA GTGCTTGCTC ACAGTCAAGG
    AACCTAGAAC AAGAGACAGC TGGAGCATCA GAATTGGGAG AAAATAAGAA ACATAAAAAA
    TCTTGCTTGG AACATAACTC TTCAGAAAAT CCATGTGGAA CCACTTCAGA AATGTTTGGA
    AGCCCTTTTC AGACATTATA TCACTTTGAG GGGAGTGGAG AAGATCTAGA AATACAGAAA
    GTAAGTACTA CTGTTAATAG CATGGCTGCC AACATCCTGG AAAATAACAG AATTCAGAAT
    CAACCAGAGA GATTTAAAGA TGCTACTGAA ATGGATTGCC AACCTCTGTC TTTTGAGACA
    GCATTATCAG GAGAACCTAG TGCTCAGAGA GAGGAAGAGA AAAGAAAAAA ACCTAGTACT
    TGTGGAAGAA TAAATGTTTT TCGTTATGGA CAAGTTAAAT TATGTTCCAC TGGCTTCATA
    ACTCATGTGG TACAGAATGA GCAAACTAAA TTAACTGAAA CAGAACATTT ATTTAAAAGT
    TATGCTCAAC CTGGTCCTGT GAGTGCCAAA GAAACATTTA GAAATAGAAC ACACCATTCA
    GTTGAGACTC CCAACAGCAA AGATTTAACC AGCACTTTAA GTAAAGAATT TGCTGAACTA
    CCCAACAAAA GTCTGTACAG CACAAATCGA AGTTATGAGC TAGAGAACAA ACATACAGCA
    GCCTGTAAAA ATTTTGCTAT TTTTCAGGAA AGTGGTAAAA AATCACACAC AGGTGGCTTA
    TTACCTAACA CATCCTCTTT TTCTTGGGGT GGACATGTTT CAGATGGTAG TAAGAAAACA
    GACAAGTTGA TTGGTCCTGC CGAACCCATA CCTCATAAGA AGCTAAGCTT AAGCTCACAA
    CTAGGATCTT TAGAGAAGTT TAAGAGGCAA TATGGGAAGG TTAAAAATCC TCTGAATACT
    GAAGTGGAGG AAAATAATAC TTTAGAAATC ACTACCAATC TCAGTCCTCA GGTTGAAGCT
    GACATTCCAT GGAAAGACAA AAACCACTTA GACAACTCTG ACATCTGTGA AATCACTACT
    ATGAAATATA ATGATTCCTA TGGTAGTTGT CAACCAGTAA ATCACATGTT TTCTTCAGAA
    AAGCTTCCAT TCTCCAAGGA AGATGATTGT TTGGAACAAC AGATGCTTTG CTTAACAGAA
    AGCACTGTAA CTCTACAGGA GTTACCTCAT TTTAACAGTA AATTTTTGGA TGTTGAGAAG
    TCACCTGAAT CACTAGCCTC TAAATTATCC AGATTGAAGG GTTCCGAAAG AGAGACTCAA
    AGGATGGATG TGAATCATTT TAATGAATTG CTACAATTAG ATTCCAGTAG GAAAGATGGT
    GACTTGTCCA GTGGGTTAAC CCTAGATTCT TGTAAGTTAT TTAAAAATGA GCATAAAAAC
    CCAGAAAGTG ACAGTATCCC AATGTCAGAC TCTGTCACAC AAGGTAATCT CTTCAGTAAA
    GACAGTGAAA CATATTCTAA CAACAATACC ACTGAGAAGA TACCAGAAAA TCCTTTGGTA
    CTGCCCTATG ACCATGCTAC AGTTATCAGT AAGGATTCAG ATATTCTTAT AGCTTCAAAA
    CAACAGATTG GAAGTCCTGA CTCTCTGAGT AGAATGTTAA TGAGTCGCGT GGAAGATTCC
    ACAGCTGACC AAAATGGAAC TTGTTTTCAG AGTGAGGAAT CCATAGCAAG AACTTGTTCT
    GAAAATGAAG AGTCCAACAT ATGTTCTTTG GATTGGCAGC AGCATTTTGA TGTAACTCTG
    GGAAGAATGG TTTACATCAA CAAACTAACA GGACTTAGCA CATTCACTGC TCCTACTGAG
    GATGTTCAGG CTGCTTGTAC TAAAGACCTG ACAACTATGG CTGTGGATGT TCTACTGGAG
    AATGGATCTC AGTACAGGTG TCATCCCTTT AGAAGCGACC TTGTTCTTCC TTTCCTTCCT
    AGAGCTCGGG AAGAGAGGAC TGTGATGAGA CAGAATAACA GAGCTACTGG GGACGATGCC
    TTTGGTCCTG AATCACTTCA ATCTTTGTTC TCAGAATGGG ACAATCCAGT ATTTGCCCGT
    TATCCAGAGG TTGCTGTTGA CGTAAGCAGC GGCCAAGCTG AGAGCCTAGC AGTTAAAATT
    CACAACATCT TGTATCCTTA TCGTTTCACC AAAGAAATGA TTCACTCAAT GCAGGTTCTC
    CAGCAAGTGG ATAACAAGTT TATTGCCTGT TTAATGAGCA CTAAGACTGA AGAGAATGGT
    GAGGCAGGTG GAAACCTGCT AGTCTTGGTG GACCAGCACG CTGCCCACGA GCGCATCCGA
    TTGGAGCAGC TGATCATTGA TTCCTATGAA AAGCAACAGC CACAAGGCTT TGGTCGAAAA
    AAATTACTGT CTTCCATTGT AAGTCCTCCA CTAGAGATAA CAGTGACAGA AGAACAAAGG
    AGACTCTTAC GGTGTTACCA CAAAAATCTG GAAGATCTGG GCCTTGAAAT TGTATTTCCA
    GACACCAGTG ATTCACTGGT CCTCATAGGA AAAGTACCAC TCTGTTTTGT AGAAAGAGAA
    GCAAGTGAAC TTCGAAGAGG AAGATCTACT GTGACCAAGG GCATTGTGGA GGAGTTTATT
    CGAGAACAAG TGGAGCTACT CCAGACCACA GGAGGCATCC AAGGGACTTT GCCACTGACT
    GTCCAGAAGG TGTTGGCATC CCAAGCCTGC CATGGGGCCA TTAAGTTTAA CGATGGCCTG
    AGCTTCGAGG AGAGCTGCCG CCTTATTGAA GCGCTGTCCT GGTGCCAGCT GCCGTTCCAG
    TGTGCTCATG GCAGGCCCTC TATGCTGCCA TTAGCTAACA TCGACCACTT GGAGCAGGAA
    AAACAGACTA AACCCAATCT TGCTAAACTT CGCAGAATGG CTCAAGCCTG GCATCTCTTT
    GGAAAGGAGA AGGATGTGAT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811470.1
    CDS9..4472
    Translation

    Target ORF information:

    RefSeq Version XM_010813168.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813168.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGTAA GTCCCAAAAG TTAAGAGAAA TAAATTTTAA ATATAAGGAG
    TTTGAGCTTA CTGGCTTTAT CAGCTCCGAA GCACATTATA ATAAGAATAT GCAGTTTTTG
    TTTGTGAACA AGAGGTTAGT TTTAAGGACA AAGTTGCATA AACTCATTGA CTTTTTATTA
    AGGAAAGAAA GTATTATATG CAAACCAAAG AGTGGTTCTG CCAGTAGGCA AGTGAATTCA
    AATCCTCGGC CTCGGTCTAA CCCGGAACTC CATGGTATCT ATGTAATCAA TGTGCAGTGC
    CAGTTCTGTG AGTATGACGT ATGCCTGGAG CCAGCAAAAA CCCTGATTGA GTTTCAGAAC
    TGGGATACTC TTTTGATTTG CATTCAGGAA GGAGTAAAAA CGTTTTTAAA GAAAGAAAAA
    TTATTTGTGG AATTATCAAG TGAAGACATT AAGGAATTTA GTGAAGATAA TGATTTTAGC
    TTGTTTAGTG CCAGTCTTCA GAAGCAAGTG TCCTCTGATG AGAAGTGTGG GCAGGTCAGT
    TTCCAAGAAG CATGTAATAA TATTTTGGAT TCCTACGAGA TGTTCAATTT GCAGTCAAAA
    GCTGTGAAAA GAAAAGCTCC TGTAGAAAAC ATAAGCACAC AAAATTCTAG GGATTCAGAA
    GCTATCAGAA AAAAGACAAA TGATTCATTT TTGTACACTT ACAAATCTGA TGGGCCAGCC
    CATTGTAAAA TGGCAGAGTC ATCTTTGCAA AATGAAGACA GTGCTTGCTC ACAGTCAAGG
    AACCTAGAAC AAGAGACAGC TGGAGCATCA GAATTGGGAG AAAATAAGAA ACATAAAAAA
    TCTTGCTTGG AACATAACTC TTCAGAAAAT CCATGTGGAA CCACTTCAGA AATGTTTGGA
    AGCCCTTTTC AGACATTATA TCACTTTGAG GGGAGTGGAG AAGATCTAGA AATACAGAAA
    GTAAGTACTA CTGTTAATAG CATGGCTGCC AACATCCTGG AAAATAACAG AATTCAGAAT
    CAACCAGAGA GATTTAAAGA TGCTACTGAA ATGGATTGCC AACCTCTGTC TTTTGAGACA
    GCATTATCAG GAGAACCTAG TGCTCAGAGA GAGGAAGAGA AAAGAAAAAA ACCTAGTACT
    TGTGGAAGAA TAAATGTTTT TCGTTATGGA CAAGTTAAAT TATGTTCCAC TGGCTTCATA
    ACTCATGTGG TACAGAATGA GCAAACTAAA TTAACTGAAA CAGAACATTT ATTTAAAAGT
    TATGCTCAAC CTGGTCCTGT GAGTGCCAAA GAAACATTTA GAAATAGAAC ACACCATTCA
    GTTGAGACTC CCAACAGCAA AGATTTAACC AGCACTTTAA GTAAAGAATT TGCTGAACTA
    CCCAACAAAA GTCTGTACAG CACAAATCGA AGTTATGAGC TAGAGAACAA ACATACAGCA
    GCCTGTAAAA ATTTTGCTAT TTTTCAGGAA AGTGGTAAAA AATCACACAC AGGTGGCTTA
    TTACCTAACA CATCCTCTTT TTCTTGGGGT GGACATGTTT CAGATGGTAG TAAGAAAACA
    GACAAGTTGA TTGGTCCTGC CGAACCCATA CCTCATAAGA AGCTAAGCTT AAGCTCACAA
    CTAGGATCTT TAGAGAAGTT TAAGAGGCAA TATGGGAAGG TTAAAAATCC TCTGAATACT
    GAAGTGGAGG AAAATAATAC TTTAGAAATC ACTACCAATC TCAGTCCTCA GGTTGAAGCT
    GACATTCCAT GGAAAGACAA AAACCACTTA GACAACTCTG ACATCTGTGA AATCACTACT
    ATGAAATATA ATGATTCCTA TGGTAGTTGT CAACCAGTAA ATCACATGTT TTCTTCAGAA
    AAGCTTCCAT TCTCCAAGGA AGATGATTGT TTGGAACAAC AGATGCTTTG CTTAACAGAA
    AGCACTGTAA CTCTACAGGA GTTACCTCAT TTTAACAGTA AATTTTTGGA TGTTGAGAAG
    TCACCTGAAT CACTAGCCTC TAAATTATCC AGATTGAAGG GTTCCGAAAG AGAGACTCAA
    AGGATGGATG TGAATCATTT TAATGAATTG CTACAATTAG ATTCCAGTAG GAAAGATGGT
    GACTTGTCCA GTGGGTTAAC CCTAGATTCT TGTAAGTTAT TTAAAAATGA GCATAAAAAC
    CCAGAAAGTG ACAGTATCCC AATGTCAGAC TCTGTCACAC AAGGTAATCT CTTCAGTAAA
    GACAGTGAAA CATATTCTAA CAACAATACC ACTGAGAAGA TACCAGAAAA TCCTTTGGTA
    CTGCCCTATG ACCATGCTAC AGTTATCAGT AAGGATTCAG ATATTCTTAT AGCTTCAAAA
    CAACAGATTG GAAGTCCTGA CTCTCTGAGT AGAATGTTAA TGAGTCGCGT GGAAGATTCC
    ACAGCTGACC AAAATGGAAC TTGTTTTCAG AGTGAGGAAT CCATAGCAAG AACTTGTTCT
    GAAAATGAAG AGTCCAACAT ATGTTCTTTG GATTGGCAGC AGCATTTTGA TGTAACTCTG
    GGAAGAATGG TTTACATCAA CAAACTAACA GGACTTAGCA CATTCACTGC TCCTACTGAG
    GATGTTCAGG CTGCTTGTAC TAAAGACCTG ACAACTATGG CTGTGGATGT TCTACTGGAG
    AATGGATCTC AGTACAGGTG TCATCCCTTT AGAAGCGACC TTGTTCTTCC TTTCCTTCCT
    AGAGCTCGGG AAGAGAGGAC TGTGATGAGA CAGAATAACA GAGCTACTGG GGACGATGCC
    TTTGGTCCTG AATCACTTCA ATCTTTGTTC TCAGAATGGG ACAATCCAGT ATTTGCCCGT
    TATCCAGAGG TTGCTGTTGA CGTAAGCAGC GGCCAAGCTG AGAGCCTAGC AGTTAAAATT
    CACAACATCT TGTATCCTTA TCGTTTCACC AAAGAAATGA TTCACTCAAT GCAGGTTCTC
    CAGCAAGTGG ATAACAAGTT TATTGCCTGT TTAATGAGCA CTAAGACTGA AGAGAATGGT
    GAGGCAGGTG GAAACCTGCT AGTCTTGGTG GACCAGCACG CTGCCCACGA GCGCATCCGA
    TTGGAGCAGC TGATCATTGA TTCCTATGAA AAGCAACAGC CACAAGGCTT TGGTCGAAAA
    AAATTACTGT CTTCCATTGT AAGTCCTCCA CTAGAGATAA CAGTGACAGA AGAACAAAGG
    AGACTCTTAC GGTGTTACCA CAAAAATCTG GAAGATCTGG GCCTTGAAAT TGTATTTCCA
    GACACCAGTG ATTCACTGGT CCTCATAGGA AAAGTACCAC TCTGTTTTGT AGAAAGAGAA
    GCAAGTGAAC TTCGAAGAGG AAGATCTACT GTGACCAAGG GCATTGTGGA GGAGTTTATT
    CGAGAACAAG TGGAGCTACT CCAGACCACA GGAGGCATCC AAGGGACTTT GCCACTGACT
    GTCCAGAAGG TGTTGGCATC CCAAGCCTGC CATGGGGCCA TTAAGTTTAA CGATGGCCTG
    AGCTTCGAGG AGAGCTGCCG CCTTATTGAA GCGCTGTCCT GGTGCCAGCT GCCGTTCCAG
    TGTGCTCATG GCAGGCCCTC TATGCTGCCA TTAGCTAACA TCGACCACTT GGAGCAGGAA
    AAACAGACTA AACCCAATCT TGCTAAACTT CGCAGAATGG CTCAAGCCTG GCATCTCTTT
    GGAAAGGAGA AGGATGTGAT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57925
    Clone ID Related Accession (Same CDS sequence) XM_010809611.2 , XM_010809611.3 , XM_010813172.2 , XM_010809606.3 , XM_010813188.2 , XM_010809606.2
    Accession Version XM_010809611.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4320bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104283.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809611.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGATCAAAT GCTTGTCAGC TGAAGTACAA GCTAGATTGC GTTCTGGTTT GGCTGTATGC 
    TCCTTAGGCC AATGTGTTGA GGAGCTTGCC CTGAACAGTA TTGATGCTGA AGCAAAGTGT
    GTGGCTGTCA GGGTGAACAT GGAAACCTTT CAAGTTCAAG TGATAGACAA TGGATTTGGG
    ATGGGGAGTG ATGATGTAGA CAAGGTGGGA AATCGTTATT TCACCAGTAA ATGCAACTCC
    ATACAGGACT TGGAGAACCC AAGGTTTTAT GGTTTCCGGG GGGAGGCGTT GGCCAGTATA
    GCTGATATGG CCAGTGCTGT GGAAATTTCA TCCAAGAAAA ACAAGTCGAT GAAAACATTT
    GTGAAACTGT TTCAGAATGG GAAAGCCCTG AAAGCTTGTG AAGCTGACTT AACTCGACCA
    AGTGCCGGGA CGACAGTCAC TGTATATAAT CTGTTTTATC AGTTACCTGT TAGGAGGAAA
    TGCATGGATC CTAGACTGGA GTTTGAGAAG GTTAGGCAGA GGATAGAAGC TCTGTCACTC
    ATGCACCCTA CCATTTCTTT CTCTTTGAGA AATGATGTTT CTGGTTCCAT GGTTCTTCAA
    CTCCCTAAAA CCAAAGACAT ATGTTCCCGA TTTTGTCAAA TTTATGGACT GGGTAAGTCC
    CAAAAGTTAA GAGAAATAAA TTTTAAATAT AAGGAGTTTG AGCTTACTGG CTTTATCAGC
    TCCGAAGCAC ATTATAATAA GAATATGCAG TTTTTGTTTG TGAACAAGAG GTTAGTTTTA
    AGGACAAAGT TGCATAAACT CATTGACTTT TTATTAAGGA AAGAAAGTAT TATATGCAAA
    CCAAAGAGTG GTTCTGCCAG TAGGCAAGTG AATTCAAATC CTCGGCCTCG GTCTAACCCG
    GAACTCCATG GTATCTATGT AATCAATGTG CAGTGCCAGT TCTGTGAGTA TGACGTATGC
    CTGGAGCCAG CAAAAACCCT GATTGAGTTT CAGAACTGGG ATACTCTTTT GATTTGCATT
    CAGGAAGGAG TAAAAACGTT TTTAAAGAAA GAAAAATTAT TTGTGGAATT ATCAAGTGAA
    GACATTAAGG AATTTAGTGA AGATAATGAT TTTAGCTTGT TTAGTGCCAG TCTTCAGAAG
    CAAGTGTCCT CTGATGAGAA GTGTGGGCAG GTCAGTTTCC AAGAAGCATG TAATAATATT
    TTGGATTCCT ACGAGATGTT CAATTTGCAG TCAAAAGCTG TGAAAAGAAA AGCTCCTGTA
    GAAAACATAA GCACACAAAA TTCTAGGGAT TCAGAAGCTA TCAGAAAAAA GACAAATGAT
    TCATTTTTGT ACACTTACAA ATCTGATGGG CCAGCCCATT GTAAAATGGC AGAGTCATCT
    TTGCAAAATG AAGACAGTGC TTGCTCACAG TCAAGGAACC TAGAACAAGA GACAGCTGGA
    GCATCAGAAT TGGGAGAAAA TAAGAAACAT AAAAAATCTT GCTTGGAACA TAACTCTTCA
    GAAAATCCAT GTGGAACCAC TTCAGAAATG TTTGGAAGCC CTTTTCAGAC ATTATATCAC
    TTTGAGGGGA GTGGAGAAGA TCTAGAAATA CAGAAAGTAA GTACTACTGT TAATAGCATG
    GCTGCCAACA TCCTGGAAAA TAACAGAATT CAGAATCAAC CAGAGAGATT TAAAGATGCT
    ACTGAAATGG ATTGCCAACC TCTGTCTTTT GAGACAGCAT TATCAGGAGA ACCTAGTGCT
    CAGAGAGAGG AAGAGAAAAG AAAAAAACCT AGTACTTGTG GAAGAATAAA TGTTTTTCGT
    TATGGACAAG TTAAATTATG TTCCACTGGC TTCATAACTC ATGTGGTACA GAATGAGCAA
    ACTAAATTAA CTGAAACAGA ACATTTATTT AAAAGTTATG CTCAACCTGG TCCTGTGAGT
    GCCAAAGAAA CATTTAGAAA TAGAACACAC CATTCAGTTG AGACTCCCAA CAGCAAAGAT
    TTAACCAGCA CTTTAAGTAA AGAATTTGCT GAACTACCCA ACAAAAGTCT GTACAGCACA
    AATCGAAGTT ATGAGCTAGA GAACAAACAT ACAGCAGCCT GTAAAAATTT TGCTATTTTT
    CAGGAAAGTG GTAAAAAATC ACACACAGGT GGCTTATTAC CTAACACATC CTCTTTTTCT
    TGGGGTGGAC ATGTTTCAGA TGGTAGTAAG AAAACAGACA AGTTGATTGG TCCTGCCGAA
    CCCATACCTC ATAAGAAGCT AAGCTTAAGC TCACAACTAG GATCTTTAGA GAAGTTTAAG
    AGGCAATATG GGAAGGTTAA AAATCCTCTG AATACTGAAG TGGAGGAAAA TAATACTTTA
    GAAATCACTA CCAATCTCAG TCCTCAGGTT GAAGCTGACA TTCCATGGAA AGACAAAAAC
    CACTTAGACA ACTCTGACAT CTGTGAAATC ACTACTATGA AATATAATGA TTCCTATGGT
    AGTTGTCAAC CAGTAAATCA CATGTTTTCT TCAGAAAAGC TTCCATTCTC CAAGGAAGAT
    GATTGTTTGG AACAACAGAT GCTTTGCTTA ACAGAAAGCA CTGTAACTCT ACAGGAGTTA
    CCTCATTTTA ACAGTAAATT TTTGGATGTT GAGAAGTCAC CTGAATCACT AGCCTCTAAA
    TTATCCAGAT TGAAGGGTTC CGAAAGAGAG ACTCAAAGGA TGGATGTGAA TCATTTTAAT
    GAATTGCTAC AATTAGATTC CAGTAGGAAA GATGGTGACT TGTCCAGTGG GTTAACCCTA
    GATTCTTGTA AGTTATTTAA AAATGAGCAT AAAAACCCAG AAAGTGACAG TATCCCAATG
    TCAGACTCTG TCACACAAGG TAATCTCTTC AGTAAAGACA GTGAAACATA TTCTAACAAC
    AATACCACTG AGAAGATACC AGAAAATCCT TTGGTACTGC CCTATGACCA TGCTACAGTT
    ATCAGTAAGG ATTCAGATAT TCTTATAGCT TCAAAACAAC AGATTGGAAG TCCTGACTCT
    CTGAGTAGAA TGTTAATGAG TCGCGTGGAA GATTCCACAG CTGACCAAAA TGGAACTTGT
    TTTCAGAGTG AGGAATCCAT AGCAAGAACT TGTTCTGAAA ATGAAGAGTC CAACATATGT
    TCTTTGGATT GGCAGCAGCA TTTTGATGTA ACTCTGGGAA GAATGGTTTA CATCAACAAA
    CTAACAGGAC TTAGCACATT CACTGCTCCT ACTGAGGATG TTCAGGCTGC TTGTACTAAA
    GACCTGACAA CTATGGCTGT GGATGTTCTA CTGGAGAATG GATCTCAGTA CAGGTGTCAT
    CCCTTTAGAA GCGACCTTGT TCTTCCTTTC CTTCCTAGAG CTCGGGAAGA GAGGACTGTG
    ATGAGACAGA ATAACAGAGC TACTGGGGAC GATGCCTTTG GTCCTGAATC ACTTCAATCT
    TTGTTCTCAG AATGGGACAA TCCAGTATTT GCCCGTTATC CAGAGGTTGC TGTTGACGTA
    AGCAGCGGCC AAGCTGAGAG CCTAGCAGTT AAAATTCACA ACATCTTGTA TCCTTATCGT
    TTCACCAAAG AAATGATTCA CTCAATGCAG GTTCTCCAGC AAGTGGATAA CAAGTTTATT
    GCCTGTTTAA TGAGCACTAA GACTGAAGAG AATGGTGAGG CAGGTGGAAA CCTGCTAGTC
    TTGGTGGACC AGCACGCTGC CCACGAGCGC ATCCGATTGG AGCAGCTGAT CATTGATTCC
    TATGAAAAGC AACAGCCACA AGGCTTTGGT CGAAAAAAAT TACTGTCTTC CATTGTAAGT
    CCTCCACTAG AGATAACAGT GACAGAAGAA CAAAGGAGAC TCTTACGGTG TTACCACAAA
    AATCTGGAAG ATCTGGGCCT TGAAATTGTA TTTCCAGACA CCAGTGATTC ACTGGTCCTC
    ATAGGAAAAG TACCACTCTG TTTTGTAGAA AGAGAAGCAA GTGAACTTCG AAGAGGAAGA
    TCTACTGTGA CCAAGGGCAT TGTGGAGGAG TTTATTCGAG AACAAGTGGA GCTACTCCAG
    ACCACAGGAG GCATCCAAGG GACTTTGCCA CTGACTGTCC AGAAGGTGTT GGCATCCCAA
    GCCTGCCATG GGGCCATTAA GTTTAACGAT GGCCTGAGCT TCGAGGAGAG CTGCCGCCTT
    ATTGAAGCGC TGTCCTGGTG CCAGCTGCCG TTCCAGTGTG CTCATGGCAG GCCCTCTATG
    CTGCCATTAG CTAACATCGA CCACTTGGAG CAGGAAAAAC AGACTAAACC CAATCTTGCT
    AAACTTCGCA GAATGGCTCA AGCCTGGCAT CTCTTTGGAA AGGAGAAGGA TGTGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807913.1
    CDS229..4548
    Translation

    Target ORF information:

    RefSeq Version XM_010809611.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809611.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGATCAAAT GCTTGTCAGC TGAAGTACAA GCTAGATTGC GTTCTGGTTT GGCTGTATGC 
    TCCTTAGGCC AATGTGTTGA GGAGCTTGCC CTGAACAGTA TTGATGCTGA AGCAAAGTGT
    GTGGCTGTCA GGGTGAACAT GGAAACCTTT CAAGTTCAAG TGATAGACAA TGGATTTGGG
    ATGGGGAGTG ATGATGTAGA CAAGGTGGGA AATCGTTATT TCACCAGTAA ATGCAACTCC
    ATACAGGACT TGGAGAACCC AAGGTTTTAT GGTTTCCGGG GGGAGGCGTT GGCCAGTATA
    GCTGATATGG CCAGTGCTGT GGAAATTTCA TCCAAGAAAA ACAAGTCGAT GAAAACATTT
    GTGAAACTGT TTCAGAATGG GAAAGCCCTG AAAGCTTGTG AAGCTGACTT AACTCGACCA
    AGTGCCGGGA CGACAGTCAC TGTATATAAT CTGTTTTATC AGTTACCTGT TAGGAGGAAA
    TGCATGGATC CTAGACTGGA GTTTGAGAAG GTTAGGCAGA GGATAGAAGC TCTGTCACTC
    ATGCACCCTA CCATTTCTTT CTCTTTGAGA AATGATGTTT CTGGTTCCAT GGTTCTTCAA
    CTCCCTAAAA CCAAAGACAT ATGTTCCCGA TTTTGTCAAA TTTATGGACT GGGTAAGTCC
    CAAAAGTTAA GAGAAATAAA TTTTAAATAT AAGGAGTTTG AGCTTACTGG CTTTATCAGC
    TCCGAAGCAC ATTATAATAA GAATATGCAG TTTTTGTTTG TGAACAAGAG GTTAGTTTTA
    AGGACAAAGT TGCATAAACT CATTGACTTT TTATTAAGGA AAGAAAGTAT TATATGCAAA
    CCAAAGAGTG GTTCTGCCAG TAGGCAAGTG AATTCAAATC CTCGGCCTCG GTCTAACCCG
    GAACTCCATG GTATCTATGT AATCAATGTG CAGTGCCAGT TCTGTGAGTA TGACGTATGC
    CTGGAGCCAG CAAAAACCCT GATTGAGTTT CAGAACTGGG ATACTCTTTT GATTTGCATT
    CAGGAAGGAG TAAAAACGTT TTTAAAGAAA GAAAAATTAT TTGTGGAATT ATCAAGTGAA
    GACATTAAGG AATTTAGTGA AGATAATGAT TTTAGCTTGT TTAGTGCCAG TCTTCAGAAG
    CAAGTGTCCT CTGATGAGAA GTGTGGGCAG GTCAGTTTCC AAGAAGCATG TAATAATATT
    TTGGATTCCT ACGAGATGTT CAATTTGCAG TCAAAAGCTG TGAAAAGAAA AGCTCCTGTA
    GAAAACATAA GCACACAAAA TTCTAGGGAT TCAGAAGCTA TCAGAAAAAA GACAAATGAT
    TCATTTTTGT ACACTTACAA ATCTGATGGG CCAGCCCATT GTAAAATGGC AGAGTCATCT
    TTGCAAAATG AAGACAGTGC TTGCTCACAG TCAAGGAACC TAGAACAAGA GACAGCTGGA
    GCATCAGAAT TGGGAGAAAA TAAGAAACAT AAAAAATCTT GCTTGGAACA TAACTCTTCA
    GAAAATCCAT GTGGAACCAC TTCAGAAATG TTTGGAAGCC CTTTTCAGAC ATTATATCAC
    TTTGAGGGGA GTGGAGAAGA TCTAGAAATA CAGAAAGTAA GTACTACTGT TAATAGCATG
    GCTGCCAACA TCCTGGAAAA TAACAGAATT CAGAATCAAC CAGAGAGATT TAAAGATGCT
    ACTGAAATGG ATTGCCAACC TCTGTCTTTT GAGACAGCAT TATCAGGAGA ACCTAGTGCT
    CAGAGAGAGG AAGAGAAAAG AAAAAAACCT AGTACTTGTG GAAGAATAAA TGTTTTTCGT
    TATGGACAAG TTAAATTATG TTCCACTGGC TTCATAACTC ATGTGGTACA GAATGAGCAA
    ACTAAATTAA CTGAAACAGA ACATTTATTT AAAAGTTATG CTCAACCTGG TCCTGTGAGT
    GCCAAAGAAA CATTTAGAAA TAGAACACAC CATTCAGTTG AGACTCCCAA CAGCAAAGAT
    TTAACCAGCA CTTTAAGTAA AGAATTTGCT GAACTACCCA ACAAAAGTCT GTACAGCACA
    AATCGAAGTT ATGAGCTAGA GAACAAACAT ACAGCAGCCT GTAAAAATTT TGCTATTTTT
    CAGGAAAGTG GTAAAAAATC ACACACAGGT GGCTTATTAC CTAACACATC CTCTTTTTCT
    TGGGGTGGAC ATGTTTCAGA TGGTAGTAAG AAAACAGACA AGTTGATTGG TCCTGCCGAA
    CCCATACCTC ATAAGAAGCT AAGCTTAAGC TCACAACTAG GATCTTTAGA GAAGTTTAAG
    AGGCAATATG GGAAGGTTAA AAATCCTCTG AATACTGAAG TGGAGGAAAA TAATACTTTA
    GAAATCACTA CCAATCTCAG TCCTCAGGTT GAAGCTGACA TTCCATGGAA AGACAAAAAC
    CACTTAGACA ACTCTGACAT CTGTGAAATC ACTACTATGA AATATAATGA TTCCTATGGT
    AGTTGTCAAC CAGTAAATCA CATGTTTTCT TCAGAAAAGC TTCCATTCTC CAAGGAAGAT
    GATTGTTTGG AACAACAGAT GCTTTGCTTA ACAGAAAGCA CTGTAACTCT ACAGGAGTTA
    CCTCATTTTA ACAGTAAATT TTTGGATGTT GAGAAGTCAC CTGAATCACT AGCCTCTAAA
    TTATCCAGAT TGAAGGGTTC CGAAAGAGAG ACTCAAAGGA TGGATGTGAA TCATTTTAAT
    GAATTGCTAC AATTAGATTC CAGTAGGAAA GATGGTGACT TGTCCAGTGG GTTAACCCTA
    GATTCTTGTA AGTTATTTAA AAATGAGCAT AAAAACCCAG AAAGTGACAG TATCCCAATG
    TCAGACTCTG TCACACAAGG TAATCTCTTC AGTAAAGACA GTGAAACATA TTCTAACAAC
    AATACCACTG AGAAGATACC AGAAAATCCT TTGGTACTGC CCTATGACCA TGCTACAGTT
    ATCAGTAAGG ATTCAGATAT TCTTATAGCT TCAAAACAAC AGATTGGAAG TCCTGACTCT
    CTGAGTAGAA TGTTAATGAG TCGCGTGGAA GATTCCACAG CTGACCAAAA TGGAACTTGT
    TTTCAGAGTG AGGAATCCAT AGCAAGAACT TGTTCTGAAA ATGAAGAGTC CAACATATGT
    TCTTTGGATT GGCAGCAGCA TTTTGATGTA ACTCTGGGAA GAATGGTTTA CATCAACAAA
    CTAACAGGAC TTAGCACATT CACTGCTCCT ACTGAGGATG TTCAGGCTGC TTGTACTAAA
    GACCTGACAA CTATGGCTGT GGATGTTCTA CTGGAGAATG GATCTCAGTA CAGGTGTCAT
    CCCTTTAGAA GCGACCTTGT TCTTCCTTTC CTTCCTAGAG CTCGGGAAGA GAGGACTGTG
    ATGAGACAGA ATAACAGAGC TACTGGGGAC GATGCCTTTG GTCCTGAATC ACTTCAATCT
    TTGTTCTCAG AATGGGACAA TCCAGTATTT GCCCGTTATC CAGAGGTTGC TGTTGACGTA
    AGCAGCGGCC AAGCTGAGAG CCTAGCAGTT AAAATTCACA ACATCTTGTA TCCTTATCGT
    TTCACCAAAG AAATGATTCA CTCAATGCAG GTTCTCCAGC AAGTGGATAA CAAGTTTATT
    GCCTGTTTAA TGAGCACTAA GACTGAAGAG AATGGTGAGG CAGGTGGAAA CCTGCTAGTC
    TTGGTGGACC AGCACGCTGC CCACGAGCGC ATCCGATTGG AGCAGCTGAT CATTGATTCC
    TATGAAAAGC AACAGCCACA AGGCTTTGGT CGAAAAAAAT TACTGTCTTC CATTGTAAGT
    CCTCCACTAG AGATAACAGT GACAGAAGAA CAAAGGAGAC TCTTACGGTG TTACCACAAA
    AATCTGGAAG ATCTGGGCCT TGAAATTGTA TTTCCAGACA CCAGTGATTC ACTGGTCCTC
    ATAGGAAAAG TACCACTCTG TTTTGTAGAA AGAGAAGCAA GTGAACTTCG AAGAGGAAGA
    TCTACTGTGA CCAAGGGCAT TGTGGAGGAG TTTATTCGAG AACAAGTGGA GCTACTCCAG
    ACCACAGGAG GCATCCAAGG GACTTTGCCA CTGACTGTCC AGAAGGTGTT GGCATCCCAA
    GCCTGCCATG GGGCCATTAA GTTTAACGAT GGCCTGAGCT TCGAGGAGAG CTGCCGCCTT
    ATTGAAGCGC TGTCCTGGTG CCAGCTGCCG TTCCAGTGTG CTCATGGCAG GCCCTCTATG
    CTGCCATTAG CTAACATCGA CCACTTGGAG CAGGAAAAAC AGACTAAACC CAATCTTGCT
    AAACTTCGCA GAATGGCTCA AGCCTGGCAT CTCTTTGGAA AGGAGAAGGA TGTGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57925
    Clone ID Related Accession (Same CDS sequence) XM_010809611.2 , XM_010809611.3 , XM_010813172.2 , XM_010809606.3 , XM_010813188.2 , XM_010809606.2
    Accession Version XM_010809611.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4320bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809611.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGATCAAAT GCTTGTCAGC TGAAGTACAA GCTAGATTGC GTTCTGGTTT GGCTGTATGC 
    TCCTTAGGCC AATGTGTTGA GGAGCTTGCC CTGAACAGTA TTGATGCTGA AGCAAAGTGT
    GTGGCTGTCA GGGTGAACAT GGAAACCTTT CAAGTTCAAG TGATAGACAA TGGATTTGGG
    ATGGGGAGTG ATGATGTAGA CAAGGTGGGA AATCGTTATT TCACCAGTAA ATGCAACTCC
    ATACAGGACT TGGAGAACCC AAGGTTTTAT GGTTTCCGGG GGGAGGCGTT GGCCAGTATA
    GCTGATATGG CCAGTGCTGT GGAAATTTCA TCCAAGAAAA ACAAGTCGAT GAAAACATTT
    GTGAAACTGT TTCAGAATGG GAAAGCCCTG AAAGCTTGTG AAGCTGACTT AACTCGACCA
    AGTGCCGGGA CGACAGTCAC TGTATATAAT CTGTTTTATC AGTTACCTGT TAGGAGGAAA
    TGCATGGATC CTAGACTGGA GTTTGAGAAG GTTAGGCAGA GGATAGAAGC TCTGTCACTC
    ATGCACCCTA CCATTTCTTT CTCTTTGAGA AATGATGTTT CTGGTTCCAT GGTTCTTCAA
    CTCCCTAAAA CCAAAGACAT ATGTTCCCGA TTTTGTCAAA TTTATGGACT GGGTAAGTCC
    CAAAAGTTAA GAGAAATAAA TTTTAAATAT AAGGAGTTTG AGCTTACTGG CTTTATCAGC
    TCCGAAGCAC ATTATAATAA GAATATGCAG TTTTTGTTTG TGAACAAGAG GTTAGTTTTA
    AGGACAAAGT TGCATAAACT CATTGACTTT TTATTAAGGA AAGAAAGTAT TATATGCAAA
    CCAAAGAGTG GTTCTGCCAG TAGGCAAGTG AATTCAAATC CTCGGCCTCG GTCTAACCCG
    GAACTCCATG GTATCTATGT AATCAATGTG CAGTGCCAGT TCTGTGAGTA TGACGTATGC
    CTGGAGCCAG CAAAAACCCT GATTGAGTTT CAGAACTGGG ATACTCTTTT GATTTGCATT
    CAGGAAGGAG TAAAAACGTT TTTAAAGAAA GAAAAATTAT TTGTGGAATT ATCAAGTGAA
    GACATTAAGG AATTTAGTGA AGATAATGAT TTTAGCTTGT TTAGTGCCAG TCTTCAGAAG
    CAAGTGTCCT CTGATGAGAA GTGTGGGCAG GTCAGTTTCC AAGAAGCATG TAATAATATT
    TTGGATTCCT ACGAGATGTT CAATTTGCAG TCAAAAGCTG TGAAAAGAAA AGCTCCTGTA
    GAAAACATAA GCACACAAAA TTCTAGGGAT TCAGAAGCTA TCAGAAAAAA GACAAATGAT
    TCATTTTTGT ACACTTACAA ATCTGATGGG CCAGCCCATT GTAAAATGGC AGAGTCATCT
    TTGCAAAATG AAGACAGTGC TTGCTCACAG TCAAGGAACC TAGAACAAGA GACAGCTGGA
    GCATCAGAAT TGGGAGAAAA TAAGAAACAT AAAAAATCTT GCTTGGAACA TAACTCTTCA
    GAAAATCCAT GTGGAACCAC TTCAGAAATG TTTGGAAGCC CTTTTCAGAC ATTATATCAC
    TTTGAGGGGA GTGGAGAAGA TCTAGAAATA CAGAAAGTAA GTACTACTGT TAATAGCATG
    GCTGCCAACA TCCTGGAAAA TAACAGAATT CAGAATCAAC CAGAGAGATT TAAAGATGCT
    ACTGAAATGG ATTGCCAACC TCTGTCTTTT GAGACAGCAT TATCAGGAGA ACCTAGTGCT
    CAGAGAGAGG AAGAGAAAAG AAAAAAACCT AGTACTTGTG GAAGAATAAA TGTTTTTCGT
    TATGGACAAG TTAAATTATG TTCCACTGGC TTCATAACTC ATGTGGTACA GAATGAGCAA
    ACTAAATTAA CTGAAACAGA ACATTTATTT AAAAGTTATG CTCAACCTGG TCCTGTGAGT
    GCCAAAGAAA CATTTAGAAA TAGAACACAC CATTCAGTTG AGACTCCCAA CAGCAAAGAT
    TTAACCAGCA CTTTAAGTAA AGAATTTGCT GAACTACCCA ACAAAAGTCT GTACAGCACA
    AATCGAAGTT ATGAGCTAGA GAACAAACAT ACAGCAGCCT GTAAAAATTT TGCTATTTTT
    CAGGAAAGTG GTAAAAAATC ACACACAGGT GGCTTATTAC CTAACACATC CTCTTTTTCT
    TGGGGTGGAC ATGTTTCAGA TGGTAGTAAG AAAACAGACA AGTTGATTGG TCCTGCCGAA
    CCCATACCTC ATAAGAAGCT AAGCTTAAGC TCACAACTAG GATCTTTAGA GAAGTTTAAG
    AGGCAATATG GGAAGGTTAA AAATCCTCTG AATACTGAAG TGGAGGAAAA TAATACTTTA
    GAAATCACTA CCAATCTCAG TCCTCAGGTT GAAGCTGACA TTCCATGGAA AGACAAAAAC
    CACTTAGACA ACTCTGACAT CTGTGAAATC ACTACTATGA AATATAATGA TTCCTATGGT
    AGTTGTCAAC CAGTAAATCA CATGTTTTCT TCAGAAAAGC TTCCATTCTC CAAGGAAGAT
    GATTGTTTGG AACAACAGAT GCTTTGCTTA ACAGAAAGCA CTGTAACTCT ACAGGAGTTA
    CCTCATTTTA ACAGTAAATT TTTGGATGTT GAGAAGTCAC CTGAATCACT AGCCTCTAAA
    TTATCCAGAT TGAAGGGTTC CGAAAGAGAG ACTCAAAGGA TGGATGTGAA TCATTTTAAT
    GAATTGCTAC AATTAGATTC CAGTAGGAAA GATGGTGACT TGTCCAGTGG GTTAACCCTA
    GATTCTTGTA AGTTATTTAA AAATGAGCAT AAAAACCCAG AAAGTGACAG TATCCCAATG
    TCAGACTCTG TCACACAAGG TAATCTCTTC AGTAAAGACA GTGAAACATA TTCTAACAAC
    AATACCACTG AGAAGATACC AGAAAATCCT TTGGTACTGC CCTATGACCA TGCTACAGTT
    ATCAGTAAGG ATTCAGATAT TCTTATAGCT TCAAAACAAC AGATTGGAAG TCCTGACTCT
    CTGAGTAGAA TGTTAATGAG TCGCGTGGAA GATTCCACAG CTGACCAAAA TGGAACTTGT
    TTTCAGAGTG AGGAATCCAT AGCAAGAACT TGTTCTGAAA ATGAAGAGTC CAACATATGT
    TCTTTGGATT GGCAGCAGCA TTTTGATGTA ACTCTGGGAA GAATGGTTTA CATCAACAAA
    CTAACAGGAC TTAGCACATT CACTGCTCCT ACTGAGGATG TTCAGGCTGC TTGTACTAAA
    GACCTGACAA CTATGGCTGT GGATGTTCTA CTGGAGAATG GATCTCAGTA CAGGTGTCAT
    CCCTTTAGAA GCGACCTTGT TCTTCCTTTC CTTCCTAGAG CTCGGGAAGA GAGGACTGTG
    ATGAGACAGA ATAACAGAGC TACTGGGGAC GATGCCTTTG GTCCTGAATC ACTTCAATCT
    TTGTTCTCAG AATGGGACAA TCCAGTATTT GCCCGTTATC CAGAGGTTGC TGTTGACGTA
    AGCAGCGGCC AAGCTGAGAG CCTAGCAGTT AAAATTCACA ACATCTTGTA TCCTTATCGT
    TTCACCAAAG AAATGATTCA CTCAATGCAG GTTCTCCAGC AAGTGGATAA CAAGTTTATT
    GCCTGTTTAA TGAGCACTAA GACTGAAGAG AATGGTGAGG CAGGTGGAAA CCTGCTAGTC
    TTGGTGGACC AGCACGCTGC CCACGAGCGC ATCCGATTGG AGCAGCTGAT CATTGATTCC
    TATGAAAAGC AACAGCCACA AGGCTTTGGT CGAAAAAAAT TACTGTCTTC CATTGTAAGT
    CCTCCACTAG AGATAACAGT GACAGAAGAA CAAAGGAGAC TCTTACGGTG TTACCACAAA
    AATCTGGAAG ATCTGGGCCT TGAAATTGTA TTTCCAGACA CCAGTGATTC ACTGGTCCTC
    ATAGGAAAAG TACCACTCTG TTTTGTAGAA AGAGAAGCAA GTGAACTTCG AAGAGGAAGA
    TCTACTGTGA CCAAGGGCAT TGTGGAGGAG TTTATTCGAG AACAAGTGGA GCTACTCCAG
    ACCACAGGAG GCATCCAAGG GACTTTGCCA CTGACTGTCC AGAAGGTGTT GGCATCCCAA
    GCCTGCCATG GGGCCATTAA GTTTAACGAT GGCCTGAGCT TCGAGGAGAG CTGCCGCCTT
    ATTGAAGCGC TGTCCTGGTG CCAGCTGCCG TTCCAGTGTG CTCATGGCAG GCCCTCTATG
    CTGCCATTAG CTAACATCGA CCACTTGGAG CAGGAAAAAC AGACTAAACC CAATCTTGCT
    AAACTTCGCA GAATGGCTCA AGCCTGGCAT CTCTTTGGAA AGGAGAAGGA TGTGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807913.1
    CDS227..4546
    Translation

    Target ORF information:

    RefSeq Version XM_010809611.3
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809611.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGATCAAAT GCTTGTCAGC TGAAGTACAA GCTAGATTGC GTTCTGGTTT GGCTGTATGC 
    TCCTTAGGCC AATGTGTTGA GGAGCTTGCC CTGAACAGTA TTGATGCTGA AGCAAAGTGT
    GTGGCTGTCA GGGTGAACAT GGAAACCTTT CAAGTTCAAG TGATAGACAA TGGATTTGGG
    ATGGGGAGTG ATGATGTAGA CAAGGTGGGA AATCGTTATT TCACCAGTAA ATGCAACTCC
    ATACAGGACT TGGAGAACCC AAGGTTTTAT GGTTTCCGGG GGGAGGCGTT GGCCAGTATA
    GCTGATATGG CCAGTGCTGT GGAAATTTCA TCCAAGAAAA ACAAGTCGAT GAAAACATTT
    GTGAAACTGT TTCAGAATGG GAAAGCCCTG AAAGCTTGTG AAGCTGACTT AACTCGACCA
    AGTGCCGGGA CGACAGTCAC TGTATATAAT CTGTTTTATC AGTTACCTGT TAGGAGGAAA
    TGCATGGATC CTAGACTGGA GTTTGAGAAG GTTAGGCAGA GGATAGAAGC TCTGTCACTC
    ATGCACCCTA CCATTTCTTT CTCTTTGAGA AATGATGTTT CTGGTTCCAT GGTTCTTCAA
    CTCCCTAAAA CCAAAGACAT ATGTTCCCGA TTTTGTCAAA TTTATGGACT GGGTAAGTCC
    CAAAAGTTAA GAGAAATAAA TTTTAAATAT AAGGAGTTTG AGCTTACTGG CTTTATCAGC
    TCCGAAGCAC ATTATAATAA GAATATGCAG TTTTTGTTTG TGAACAAGAG GTTAGTTTTA
    AGGACAAAGT TGCATAAACT CATTGACTTT TTATTAAGGA AAGAAAGTAT TATATGCAAA
    CCAAAGAGTG GTTCTGCCAG TAGGCAAGTG AATTCAAATC CTCGGCCTCG GTCTAACCCG
    GAACTCCATG GTATCTATGT AATCAATGTG CAGTGCCAGT TCTGTGAGTA TGACGTATGC
    CTGGAGCCAG CAAAAACCCT GATTGAGTTT CAGAACTGGG ATACTCTTTT GATTTGCATT
    CAGGAAGGAG TAAAAACGTT TTTAAAGAAA GAAAAATTAT TTGTGGAATT ATCAAGTGAA
    GACATTAAGG AATTTAGTGA AGATAATGAT TTTAGCTTGT TTAGTGCCAG TCTTCAGAAG
    CAAGTGTCCT CTGATGAGAA GTGTGGGCAG GTCAGTTTCC AAGAAGCATG TAATAATATT
    TTGGATTCCT ACGAGATGTT CAATTTGCAG TCAAAAGCTG TGAAAAGAAA AGCTCCTGTA
    GAAAACATAA GCACACAAAA TTCTAGGGAT TCAGAAGCTA TCAGAAAAAA GACAAATGAT
    TCATTTTTGT ACACTTACAA ATCTGATGGG CCAGCCCATT GTAAAATGGC AGAGTCATCT
    TTGCAAAATG AAGACAGTGC TTGCTCACAG TCAAGGAACC TAGAACAAGA GACAGCTGGA
    GCATCAGAAT TGGGAGAAAA TAAGAAACAT AAAAAATCTT GCTTGGAACA TAACTCTTCA
    GAAAATCCAT GTGGAACCAC TTCAGAAATG TTTGGAAGCC CTTTTCAGAC ATTATATCAC
    TTTGAGGGGA GTGGAGAAGA TCTAGAAATA CAGAAAGTAA GTACTACTGT TAATAGCATG
    GCTGCCAACA TCCTGGAAAA TAACAGAATT CAGAATCAAC CAGAGAGATT TAAAGATGCT
    ACTGAAATGG ATTGCCAACC TCTGTCTTTT GAGACAGCAT TATCAGGAGA ACCTAGTGCT
    CAGAGAGAGG AAGAGAAAAG AAAAAAACCT AGTACTTGTG GAAGAATAAA TGTTTTTCGT
    TATGGACAAG TTAAATTATG TTCCACTGGC TTCATAACTC ATGTGGTACA GAATGAGCAA
    ACTAAATTAA CTGAAACAGA ACATTTATTT AAAAGTTATG CTCAACCTGG TCCTGTGAGT
    GCCAAAGAAA CATTTAGAAA TAGAACACAC CATTCAGTTG AGACTCCCAA CAGCAAAGAT
    TTAACCAGCA CTTTAAGTAA AGAATTTGCT GAACTACCCA ACAAAAGTCT GTACAGCACA
    AATCGAAGTT ATGAGCTAGA GAACAAACAT ACAGCAGCCT GTAAAAATTT TGCTATTTTT
    CAGGAAAGTG GTAAAAAATC ACACACAGGT GGCTTATTAC CTAACACATC CTCTTTTTCT
    TGGGGTGGAC ATGTTTCAGA TGGTAGTAAG AAAACAGACA AGTTGATTGG TCCTGCCGAA
    CCCATACCTC ATAAGAAGCT AAGCTTAAGC TCACAACTAG GATCTTTAGA GAAGTTTAAG
    AGGCAATATG GGAAGGTTAA AAATCCTCTG AATACTGAAG TGGAGGAAAA TAATACTTTA
    GAAATCACTA CCAATCTCAG TCCTCAGGTT GAAGCTGACA TTCCATGGAA AGACAAAAAC
    CACTTAGACA ACTCTGACAT CTGTGAAATC ACTACTATGA AATATAATGA TTCCTATGGT
    AGTTGTCAAC CAGTAAATCA CATGTTTTCT TCAGAAAAGC TTCCATTCTC CAAGGAAGAT
    GATTGTTTGG AACAACAGAT GCTTTGCTTA ACAGAAAGCA CTGTAACTCT ACAGGAGTTA
    CCTCATTTTA ACAGTAAATT TTTGGATGTT GAGAAGTCAC CTGAATCACT AGCCTCTAAA
    TTATCCAGAT TGAAGGGTTC CGAAAGAGAG ACTCAAAGGA TGGATGTGAA TCATTTTAAT
    GAATTGCTAC AATTAGATTC CAGTAGGAAA GATGGTGACT TGTCCAGTGG GTTAACCCTA
    GATTCTTGTA AGTTATTTAA AAATGAGCAT AAAAACCCAG AAAGTGACAG TATCCCAATG
    TCAGACTCTG TCACACAAGG TAATCTCTTC AGTAAAGACA GTGAAACATA TTCTAACAAC
    AATACCACTG AGAAGATACC AGAAAATCCT TTGGTACTGC CCTATGACCA TGCTACAGTT
    ATCAGTAAGG ATTCAGATAT TCTTATAGCT TCAAAACAAC AGATTGGAAG TCCTGACTCT
    CTGAGTAGAA TGTTAATGAG TCGCGTGGAA GATTCCACAG CTGACCAAAA TGGAACTTGT
    TTTCAGAGTG AGGAATCCAT AGCAAGAACT TGTTCTGAAA ATGAAGAGTC CAACATATGT
    TCTTTGGATT GGCAGCAGCA TTTTGATGTA ACTCTGGGAA GAATGGTTTA CATCAACAAA
    CTAACAGGAC TTAGCACATT CACTGCTCCT ACTGAGGATG TTCAGGCTGC TTGTACTAAA
    GACCTGACAA CTATGGCTGT GGATGTTCTA CTGGAGAATG GATCTCAGTA CAGGTGTCAT
    CCCTTTAGAA GCGACCTTGT TCTTCCTTTC CTTCCTAGAG CTCGGGAAGA GAGGACTGTG
    ATGAGACAGA ATAACAGAGC TACTGGGGAC GATGCCTTTG GTCCTGAATC ACTTCAATCT
    TTGTTCTCAG AATGGGACAA TCCAGTATTT GCCCGTTATC CAGAGGTTGC TGTTGACGTA
    AGCAGCGGCC AAGCTGAGAG CCTAGCAGTT AAAATTCACA ACATCTTGTA TCCTTATCGT
    TTCACCAAAG AAATGATTCA CTCAATGCAG GTTCTCCAGC AAGTGGATAA CAAGTTTATT
    GCCTGTTTAA TGAGCACTAA GACTGAAGAG AATGGTGAGG CAGGTGGAAA CCTGCTAGTC
    TTGGTGGACC AGCACGCTGC CCACGAGCGC ATCCGATTGG AGCAGCTGAT CATTGATTCC
    TATGAAAAGC AACAGCCACA AGGCTTTGGT CGAAAAAAAT TACTGTCTTC CATTGTAAGT
    CCTCCACTAG AGATAACAGT GACAGAAGAA CAAAGGAGAC TCTTACGGTG TTACCACAAA
    AATCTGGAAG ATCTGGGCCT TGAAATTGTA TTTCCAGACA CCAGTGATTC ACTGGTCCTC
    ATAGGAAAAG TACCACTCTG TTTTGTAGAA AGAGAAGCAA GTGAACTTCG AAGAGGAAGA
    TCTACTGTGA CCAAGGGCAT TGTGGAGGAG TTTATTCGAG AACAAGTGGA GCTACTCCAG
    ACCACAGGAG GCATCCAAGG GACTTTGCCA CTGACTGTCC AGAAGGTGTT GGCATCCCAA
    GCCTGCCATG GGGCCATTAA GTTTAACGAT GGCCTGAGCT TCGAGGAGAG CTGCCGCCTT
    ATTGAAGCGC TGTCCTGGTG CCAGCTGCCG TTCCAGTGTG CTCATGGCAG GCCCTCTATG
    CTGCCATTAG CTAACATCGA CCACTTGGAG CAGGAAAAAC AGACTAAACC CAATCTTGCT
    AAACTTCGCA GAATGGCTCA AGCCTGGCAT CTCTTTGGAA AGGAGAAGGA TGTGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57925
    Clone ID Related Accession (Same CDS sequence) XM_010809611.2 , XM_010809611.3 , XM_010813172.2 , XM_010809606.3 , XM_010813188.2 , XM_010809606.2
    Accession Version XM_010813172.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4320bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGATCAAAT GCTTGTCAGC TGAAGTACAA GCTAGATTGC GTTCTGGTTT GGCTGTATGC 
    TCCTTAGGCC AATGTGTTGA GGAGCTTGCC CTGAACAGTA TTGATGCTGA AGCAAAGTGT
    GTGGCTGTCA GGGTGAACAT GGAAACCTTT CAAGTTCAAG TGATAGACAA TGGATTTGGG
    ATGGGGAGTG ATGATGTAGA CAAGGTGGGA AATCGTTATT TCACCAGTAA ATGCAACTCC
    ATACAGGACT TGGAGAACCC AAGGTTTTAT GGTTTCCGGG GGGAGGCGTT GGCCAGTATA
    GCTGATATGG CCAGTGCTGT GGAAATTTCA TCCAAGAAAA ACAAGTCGAT GAAAACATTT
    GTGAAACTGT TTCAGAATGG GAAAGCCCTG AAAGCTTGTG AAGCTGACTT AACTCGACCA
    AGTGCCGGGA CGACAGTCAC TGTATATAAT CTGTTTTATC AGTTACCTGT TAGGAGGAAA
    TGCATGGATC CTAGACTGGA GTTTGAGAAG GTTAGGCAGA GGATAGAAGC TCTGTCACTC
    ATGCACCCTA CCATTTCTTT CTCTTTGAGA AATGATGTTT CTGGTTCCAT GGTTCTTCAA
    CTCCCTAAAA CCAAAGACAT ATGTTCCCGA TTTTGTCAAA TTTATGGACT GGGTAAGTCC
    CAAAAGTTAA GAGAAATAAA TTTTAAATAT AAGGAGTTTG AGCTTACTGG CTTTATCAGC
    TCCGAAGCAC ATTATAATAA GAATATGCAG TTTTTGTTTG TGAACAAGAG GTTAGTTTTA
    AGGACAAAGT TGCATAAACT CATTGACTTT TTATTAAGGA AAGAAAGTAT TATATGCAAA
    CCAAAGAGTG GTTCTGCCAG TAGGCAAGTG AATTCAAATC CTCGGCCTCG GTCTAACCCG
    GAACTCCATG GTATCTATGT AATCAATGTG CAGTGCCAGT TCTGTGAGTA TGACGTATGC
    CTGGAGCCAG CAAAAACCCT GATTGAGTTT CAGAACTGGG ATACTCTTTT GATTTGCATT
    CAGGAAGGAG TAAAAACGTT TTTAAAGAAA GAAAAATTAT TTGTGGAATT ATCAAGTGAA
    GACATTAAGG AATTTAGTGA AGATAATGAT TTTAGCTTGT TTAGTGCCAG TCTTCAGAAG
    CAAGTGTCCT CTGATGAGAA GTGTGGGCAG GTCAGTTTCC AAGAAGCATG TAATAATATT
    TTGGATTCCT ACGAGATGTT CAATTTGCAG TCAAAAGCTG TGAAAAGAAA AGCTCCTGTA
    GAAAACATAA GCACACAAAA TTCTAGGGAT TCAGAAGCTA TCAGAAAAAA GACAAATGAT
    TCATTTTTGT ACACTTACAA ATCTGATGGG CCAGCCCATT GTAAAATGGC AGAGTCATCT
    TTGCAAAATG AAGACAGTGC TTGCTCACAG TCAAGGAACC TAGAACAAGA GACAGCTGGA
    GCATCAGAAT TGGGAGAAAA TAAGAAACAT AAAAAATCTT GCTTGGAACA TAACTCTTCA
    GAAAATCCAT GTGGAACCAC TTCAGAAATG TTTGGAAGCC CTTTTCAGAC ATTATATCAC
    TTTGAGGGGA GTGGAGAAGA TCTAGAAATA CAGAAAGTAA GTACTACTGT TAATAGCATG
    GCTGCCAACA TCCTGGAAAA TAACAGAATT CAGAATCAAC CAGAGAGATT TAAAGATGCT
    ACTGAAATGG ATTGCCAACC TCTGTCTTTT GAGACAGCAT TATCAGGAGA ACCTAGTGCT
    CAGAGAGAGG AAGAGAAAAG AAAAAAACCT AGTACTTGTG GAAGAATAAA TGTTTTTCGT
    TATGGACAAG TTAAATTATG TTCCACTGGC TTCATAACTC ATGTGGTACA GAATGAGCAA
    ACTAAATTAA CTGAAACAGA ACATTTATTT AAAAGTTATG CTCAACCTGG TCCTGTGAGT
    GCCAAAGAAA CATTTAGAAA TAGAACACAC CATTCAGTTG AGACTCCCAA CAGCAAAGAT
    TTAACCAGCA CTTTAAGTAA AGAATTTGCT GAACTACCCA ACAAAAGTCT GTACAGCACA
    AATCGAAGTT ATGAGCTAGA GAACAAACAT ACAGCAGCCT GTAAAAATTT TGCTATTTTT
    CAGGAAAGTG GTAAAAAATC ACACACAGGT GGCTTATTAC CTAACACATC CTCTTTTTCT
    TGGGGTGGAC ATGTTTCAGA TGGTAGTAAG AAAACAGACA AGTTGATTGG TCCTGCCGAA
    CCCATACCTC ATAAGAAGCT AAGCTTAAGC TCACAACTAG GATCTTTAGA GAAGTTTAAG
    AGGCAATATG GGAAGGTTAA AAATCCTCTG AATACTGAAG TGGAGGAAAA TAATACTTTA
    GAAATCACTA CCAATCTCAG TCCTCAGGTT GAAGCTGACA TTCCATGGAA AGACAAAAAC
    CACTTAGACA ACTCTGACAT CTGTGAAATC ACTACTATGA AATATAATGA TTCCTATGGT
    AGTTGTCAAC CAGTAAATCA CATGTTTTCT TCAGAAAAGC TTCCATTCTC CAAGGAAGAT
    GATTGTTTGG AACAACAGAT GCTTTGCTTA ACAGAAAGCA CTGTAACTCT ACAGGAGTTA
    CCTCATTTTA ACAGTAAATT TTTGGATGTT GAGAAGTCAC CTGAATCACT AGCCTCTAAA
    TTATCCAGAT TGAAGGGTTC CGAAAGAGAG ACTCAAAGGA TGGATGTGAA TCATTTTAAT
    GAATTGCTAC AATTAGATTC CAGTAGGAAA GATGGTGACT TGTCCAGTGG GTTAACCCTA
    GATTCTTGTA AGTTATTTAA AAATGAGCAT AAAAACCCAG AAAGTGACAG TATCCCAATG
    TCAGACTCTG TCACACAAGG TAATCTCTTC AGTAAAGACA GTGAAACATA TTCTAACAAC
    AATACCACTG AGAAGATACC AGAAAATCCT TTGGTACTGC CCTATGACCA TGCTACAGTT
    ATCAGTAAGG ATTCAGATAT TCTTATAGCT TCAAAACAAC AGATTGGAAG TCCTGACTCT
    CTGAGTAGAA TGTTAATGAG TCGCGTGGAA GATTCCACAG CTGACCAAAA TGGAACTTGT
    TTTCAGAGTG AGGAATCCAT AGCAAGAACT TGTTCTGAAA ATGAAGAGTC CAACATATGT
    TCTTTGGATT GGCAGCAGCA TTTTGATGTA ACTCTGGGAA GAATGGTTTA CATCAACAAA
    CTAACAGGAC TTAGCACATT CACTGCTCCT ACTGAGGATG TTCAGGCTGC TTGTACTAAA
    GACCTGACAA CTATGGCTGT GGATGTTCTA CTGGAGAATG GATCTCAGTA CAGGTGTCAT
    CCCTTTAGAA GCGACCTTGT TCTTCCTTTC CTTCCTAGAG CTCGGGAAGA GAGGACTGTG
    ATGAGACAGA ATAACAGAGC TACTGGGGAC GATGCCTTTG GTCCTGAATC ACTTCAATCT
    TTGTTCTCAG AATGGGACAA TCCAGTATTT GCCCGTTATC CAGAGGTTGC TGTTGACGTA
    AGCAGCGGCC AAGCTGAGAG CCTAGCAGTT AAAATTCACA ACATCTTGTA TCCTTATCGT
    TTCACCAAAG AAATGATTCA CTCAATGCAG GTTCTCCAGC AAGTGGATAA CAAGTTTATT
    GCCTGTTTAA TGAGCACTAA GACTGAAGAG AATGGTGAGG CAGGTGGAAA CCTGCTAGTC
    TTGGTGGACC AGCACGCTGC CCACGAGCGC ATCCGATTGG AGCAGCTGAT CATTGATTCC
    TATGAAAAGC AACAGCCACA AGGCTTTGGT CGAAAAAAAT TACTGTCTTC CATTGTAAGT
    CCTCCACTAG AGATAACAGT GACAGAAGAA CAAAGGAGAC TCTTACGGTG TTACCACAAA
    AATCTGGAAG ATCTGGGCCT TGAAATTGTA TTTCCAGACA CCAGTGATTC ACTGGTCCTC
    ATAGGAAAAG TACCACTCTG TTTTGTAGAA AGAGAAGCAA GTGAACTTCG AAGAGGAAGA
    TCTACTGTGA CCAAGGGCAT TGTGGAGGAG TTTATTCGAG AACAAGTGGA GCTACTCCAG
    ACCACAGGAG GCATCCAAGG GACTTTGCCA CTGACTGTCC AGAAGGTGTT GGCATCCCAA
    GCCTGCCATG GGGCCATTAA GTTTAACGAT GGCCTGAGCT TCGAGGAGAG CTGCCGCCTT
    ATTGAAGCGC TGTCCTGGTG CCAGCTGCCG TTCCAGTGTG CTCATGGCAG GCCCTCTATG
    CTGCCATTAG CTAACATCGA CCACTTGGAG CAGGAAAAAC AGACTAAACC CAATCTTGCT
    AAACTTCGCA GAATGGCTCA AGCCTGGCAT CTCTTTGGAA AGGAGAAGGA TGTGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811474.1
    CDS185..4504
    Translation

    Target ORF information:

    RefSeq Version XM_010813172.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813172.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGATCAAAT GCTTGTCAGC TGAAGTACAA GCTAGATTGC GTTCTGGTTT GGCTGTATGC 
    TCCTTAGGCC AATGTGTTGA GGAGCTTGCC CTGAACAGTA TTGATGCTGA AGCAAAGTGT
    GTGGCTGTCA GGGTGAACAT GGAAACCTTT CAAGTTCAAG TGATAGACAA TGGATTTGGG
    ATGGGGAGTG ATGATGTAGA CAAGGTGGGA AATCGTTATT TCACCAGTAA ATGCAACTCC
    ATACAGGACT TGGAGAACCC AAGGTTTTAT GGTTTCCGGG GGGAGGCGTT GGCCAGTATA
    GCTGATATGG CCAGTGCTGT GGAAATTTCA TCCAAGAAAA ACAAGTCGAT GAAAACATTT
    GTGAAACTGT TTCAGAATGG GAAAGCCCTG AAAGCTTGTG AAGCTGACTT AACTCGACCA
    AGTGCCGGGA CGACAGTCAC TGTATATAAT CTGTTTTATC AGTTACCTGT TAGGAGGAAA
    TGCATGGATC CTAGACTGGA GTTTGAGAAG GTTAGGCAGA GGATAGAAGC TCTGTCACTC
    ATGCACCCTA CCATTTCTTT CTCTTTGAGA AATGATGTTT CTGGTTCCAT GGTTCTTCAA
    CTCCCTAAAA CCAAAGACAT ATGTTCCCGA TTTTGTCAAA TTTATGGACT GGGTAAGTCC
    CAAAAGTTAA GAGAAATAAA TTTTAAATAT AAGGAGTTTG AGCTTACTGG CTTTATCAGC
    TCCGAAGCAC ATTATAATAA GAATATGCAG TTTTTGTTTG TGAACAAGAG GTTAGTTTTA
    AGGACAAAGT TGCATAAACT CATTGACTTT TTATTAAGGA AAGAAAGTAT TATATGCAAA
    CCAAAGAGTG GTTCTGCCAG TAGGCAAGTG AATTCAAATC CTCGGCCTCG GTCTAACCCG
    GAACTCCATG GTATCTATGT AATCAATGTG CAGTGCCAGT TCTGTGAGTA TGACGTATGC
    CTGGAGCCAG CAAAAACCCT GATTGAGTTT CAGAACTGGG ATACTCTTTT GATTTGCATT
    CAGGAAGGAG TAAAAACGTT TTTAAAGAAA GAAAAATTAT TTGTGGAATT ATCAAGTGAA
    GACATTAAGG AATTTAGTGA AGATAATGAT TTTAGCTTGT TTAGTGCCAG TCTTCAGAAG
    CAAGTGTCCT CTGATGAGAA GTGTGGGCAG GTCAGTTTCC AAGAAGCATG TAATAATATT
    TTGGATTCCT ACGAGATGTT CAATTTGCAG TCAAAAGCTG TGAAAAGAAA AGCTCCTGTA
    GAAAACATAA GCACACAAAA TTCTAGGGAT TCAGAAGCTA TCAGAAAAAA GACAAATGAT
    TCATTTTTGT ACACTTACAA ATCTGATGGG CCAGCCCATT GTAAAATGGC AGAGTCATCT
    TTGCAAAATG AAGACAGTGC TTGCTCACAG TCAAGGAACC TAGAACAAGA GACAGCTGGA
    GCATCAGAAT TGGGAGAAAA TAAGAAACAT AAAAAATCTT GCTTGGAACA TAACTCTTCA
    GAAAATCCAT GTGGAACCAC TTCAGAAATG TTTGGAAGCC CTTTTCAGAC ATTATATCAC
    TTTGAGGGGA GTGGAGAAGA TCTAGAAATA CAGAAAGTAA GTACTACTGT TAATAGCATG
    GCTGCCAACA TCCTGGAAAA TAACAGAATT CAGAATCAAC CAGAGAGATT TAAAGATGCT
    ACTGAAATGG ATTGCCAACC TCTGTCTTTT GAGACAGCAT TATCAGGAGA ACCTAGTGCT
    CAGAGAGAGG AAGAGAAAAG AAAAAAACCT AGTACTTGTG GAAGAATAAA TGTTTTTCGT
    TATGGACAAG TTAAATTATG TTCCACTGGC TTCATAACTC ATGTGGTACA GAATGAGCAA
    ACTAAATTAA CTGAAACAGA ACATTTATTT AAAAGTTATG CTCAACCTGG TCCTGTGAGT
    GCCAAAGAAA CATTTAGAAA TAGAACACAC CATTCAGTTG AGACTCCCAA CAGCAAAGAT
    TTAACCAGCA CTTTAAGTAA AGAATTTGCT GAACTACCCA ACAAAAGTCT GTACAGCACA
    AATCGAAGTT ATGAGCTAGA GAACAAACAT ACAGCAGCCT GTAAAAATTT TGCTATTTTT
    CAGGAAAGTG GTAAAAAATC ACACACAGGT GGCTTATTAC CTAACACATC CTCTTTTTCT
    TGGGGTGGAC ATGTTTCAGA TGGTAGTAAG AAAACAGACA AGTTGATTGG TCCTGCCGAA
    CCCATACCTC ATAAGAAGCT AAGCTTAAGC TCACAACTAG GATCTTTAGA GAAGTTTAAG
    AGGCAATATG GGAAGGTTAA AAATCCTCTG AATACTGAAG TGGAGGAAAA TAATACTTTA
    GAAATCACTA CCAATCTCAG TCCTCAGGTT GAAGCTGACA TTCCATGGAA AGACAAAAAC
    CACTTAGACA ACTCTGACAT CTGTGAAATC ACTACTATGA AATATAATGA TTCCTATGGT
    AGTTGTCAAC CAGTAAATCA CATGTTTTCT TCAGAAAAGC TTCCATTCTC CAAGGAAGAT
    GATTGTTTGG AACAACAGAT GCTTTGCTTA ACAGAAAGCA CTGTAACTCT ACAGGAGTTA
    CCTCATTTTA ACAGTAAATT TTTGGATGTT GAGAAGTCAC CTGAATCACT AGCCTCTAAA
    TTATCCAGAT TGAAGGGTTC CGAAAGAGAG ACTCAAAGGA TGGATGTGAA TCATTTTAAT
    GAATTGCTAC AATTAGATTC CAGTAGGAAA GATGGTGACT TGTCCAGTGG GTTAACCCTA
    GATTCTTGTA AGTTATTTAA AAATGAGCAT AAAAACCCAG AAAGTGACAG TATCCCAATG
    TCAGACTCTG TCACACAAGG TAATCTCTTC AGTAAAGACA GTGAAACATA TTCTAACAAC
    AATACCACTG AGAAGATACC AGAAAATCCT TTGGTACTGC CCTATGACCA TGCTACAGTT
    ATCAGTAAGG ATTCAGATAT TCTTATAGCT TCAAAACAAC AGATTGGAAG TCCTGACTCT
    CTGAGTAGAA TGTTAATGAG TCGCGTGGAA GATTCCACAG CTGACCAAAA TGGAACTTGT
    TTTCAGAGTG AGGAATCCAT AGCAAGAACT TGTTCTGAAA ATGAAGAGTC CAACATATGT
    TCTTTGGATT GGCAGCAGCA TTTTGATGTA ACTCTGGGAA GAATGGTTTA CATCAACAAA
    CTAACAGGAC TTAGCACATT CACTGCTCCT ACTGAGGATG TTCAGGCTGC TTGTACTAAA
    GACCTGACAA CTATGGCTGT GGATGTTCTA CTGGAGAATG GATCTCAGTA CAGGTGTCAT
    CCCTTTAGAA GCGACCTTGT TCTTCCTTTC CTTCCTAGAG CTCGGGAAGA GAGGACTGTG
    ATGAGACAGA ATAACAGAGC TACTGGGGAC GATGCCTTTG GTCCTGAATC ACTTCAATCT
    TTGTTCTCAG AATGGGACAA TCCAGTATTT GCCCGTTATC CAGAGGTTGC TGTTGACGTA
    AGCAGCGGCC AAGCTGAGAG CCTAGCAGTT AAAATTCACA ACATCTTGTA TCCTTATCGT
    TTCACCAAAG AAATGATTCA CTCAATGCAG GTTCTCCAGC AAGTGGATAA CAAGTTTATT
    GCCTGTTTAA TGAGCACTAA GACTGAAGAG AATGGTGAGG CAGGTGGAAA CCTGCTAGTC
    TTGGTGGACC AGCACGCTGC CCACGAGCGC ATCCGATTGG AGCAGCTGAT CATTGATTCC
    TATGAAAAGC AACAGCCACA AGGCTTTGGT CGAAAAAAAT TACTGTCTTC CATTGTAAGT
    CCTCCACTAG AGATAACAGT GACAGAAGAA CAAAGGAGAC TCTTACGGTG TTACCACAAA
    AATCTGGAAG ATCTGGGCCT TGAAATTGTA TTTCCAGACA CCAGTGATTC ACTGGTCCTC
    ATAGGAAAAG TACCACTCTG TTTTGTAGAA AGAGAAGCAA GTGAACTTCG AAGAGGAAGA
    TCTACTGTGA CCAAGGGCAT TGTGGAGGAG TTTATTCGAG AACAAGTGGA GCTACTCCAG
    ACCACAGGAG GCATCCAAGG GACTTTGCCA CTGACTGTCC AGAAGGTGTT GGCATCCCAA
    GCCTGCCATG GGGCCATTAA GTTTAACGAT GGCCTGAGCT TCGAGGAGAG CTGCCGCCTT
    ATTGAAGCGC TGTCCTGGTG CCAGCTGCCG TTCCAGTGTG CTCATGGCAG GCCCTCTATG
    CTGCCATTAG CTAACATCGA CCACTTGGAG CAGGAAAAAC AGACTAAACC CAATCTTGCT
    AAACTTCGCA GAATGGCTCA AGCCTGGCAT CTCTTTGGAA AGGAGAAGGA TGTGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57925
    Clone ID Related Accession (Same CDS sequence) XM_010809611.2 , XM_010809611.3 , XM_010813172.2 , XM_010809606.3 , XM_010813188.2 , XM_010809606.2
    Accession Version XM_010809606.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4320bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809606.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGATCAAAT GCTTGTCAGC TGAAGTACAA GCTAGATTGC GTTCTGGTTT GGCTGTATGC 
    TCCTTAGGCC AATGTGTTGA GGAGCTTGCC CTGAACAGTA TTGATGCTGA AGCAAAGTGT
    GTGGCTGTCA GGGTGAACAT GGAAACCTTT CAAGTTCAAG TGATAGACAA TGGATTTGGG
    ATGGGGAGTG ATGATGTAGA CAAGGTGGGA AATCGTTATT TCACCAGTAA ATGCAACTCC
    ATACAGGACT TGGAGAACCC AAGGTTTTAT GGTTTCCGGG GGGAGGCGTT GGCCAGTATA
    GCTGATATGG CCAGTGCTGT GGAAATTTCA TCCAAGAAAA ACAAGTCGAT GAAAACATTT
    GTGAAACTGT TTCAGAATGG GAAAGCCCTG AAAGCTTGTG AAGCTGACTT AACTCGACCA
    AGTGCCGGGA CGACAGTCAC TGTATATAAT CTGTTTTATC AGTTACCTGT TAGGAGGAAA
    TGCATGGATC CTAGACTGGA GTTTGAGAAG GTTAGGCAGA GGATAGAAGC TCTGTCACTC
    ATGCACCCTA CCATTTCTTT CTCTTTGAGA AATGATGTTT CTGGTTCCAT GGTTCTTCAA
    CTCCCTAAAA CCAAAGACAT ATGTTCCCGA TTTTGTCAAA TTTATGGACT GGGTAAGTCC
    CAAAAGTTAA GAGAAATAAA TTTTAAATAT AAGGAGTTTG AGCTTACTGG CTTTATCAGC
    TCCGAAGCAC ATTATAATAA GAATATGCAG TTTTTGTTTG TGAACAAGAG GTTAGTTTTA
    AGGACAAAGT TGCATAAACT CATTGACTTT TTATTAAGGA AAGAAAGTAT TATATGCAAA
    CCAAAGAGTG GTTCTGCCAG TAGGCAAGTG AATTCAAATC CTCGGCCTCG GTCTAACCCG
    GAACTCCATG GTATCTATGT AATCAATGTG CAGTGCCAGT TCTGTGAGTA TGACGTATGC
    CTGGAGCCAG CAAAAACCCT GATTGAGTTT CAGAACTGGG ATACTCTTTT GATTTGCATT
    CAGGAAGGAG TAAAAACGTT TTTAAAGAAA GAAAAATTAT TTGTGGAATT ATCAAGTGAA
    GACATTAAGG AATTTAGTGA AGATAATGAT TTTAGCTTGT TTAGTGCCAG TCTTCAGAAG
    CAAGTGTCCT CTGATGAGAA GTGTGGGCAG GTCAGTTTCC AAGAAGCATG TAATAATATT
    TTGGATTCCT ACGAGATGTT CAATTTGCAG TCAAAAGCTG TGAAAAGAAA AGCTCCTGTA
    GAAAACATAA GCACACAAAA TTCTAGGGAT TCAGAAGCTA TCAGAAAAAA GACAAATGAT
    TCATTTTTGT ACACTTACAA ATCTGATGGG CCAGCCCATT GTAAAATGGC AGAGTCATCT
    TTGCAAAATG AAGACAGTGC TTGCTCACAG TCAAGGAACC TAGAACAAGA GACAGCTGGA
    GCATCAGAAT TGGGAGAAAA TAAGAAACAT AAAAAATCTT GCTTGGAACA TAACTCTTCA
    GAAAATCCAT GTGGAACCAC TTCAGAAATG TTTGGAAGCC CTTTTCAGAC ATTATATCAC
    TTTGAGGGGA GTGGAGAAGA TCTAGAAATA CAGAAAGTAA GTACTACTGT TAATAGCATG
    GCTGCCAACA TCCTGGAAAA TAACAGAATT CAGAATCAAC CAGAGAGATT TAAAGATGCT
    ACTGAAATGG ATTGCCAACC TCTGTCTTTT GAGACAGCAT TATCAGGAGA ACCTAGTGCT
    CAGAGAGAGG AAGAGAAAAG AAAAAAACCT AGTACTTGTG GAAGAATAAA TGTTTTTCGT
    TATGGACAAG TTAAATTATG TTCCACTGGC TTCATAACTC ATGTGGTACA GAATGAGCAA
    ACTAAATTAA CTGAAACAGA ACATTTATTT AAAAGTTATG CTCAACCTGG TCCTGTGAGT
    GCCAAAGAAA CATTTAGAAA TAGAACACAC CATTCAGTTG AGACTCCCAA CAGCAAAGAT
    TTAACCAGCA CTTTAAGTAA AGAATTTGCT GAACTACCCA ACAAAAGTCT GTACAGCACA
    AATCGAAGTT ATGAGCTAGA GAACAAACAT ACAGCAGCCT GTAAAAATTT TGCTATTTTT
    CAGGAAAGTG GTAAAAAATC ACACACAGGT GGCTTATTAC CTAACACATC CTCTTTTTCT
    TGGGGTGGAC ATGTTTCAGA TGGTAGTAAG AAAACAGACA AGTTGATTGG TCCTGCCGAA
    CCCATACCTC ATAAGAAGCT AAGCTTAAGC TCACAACTAG GATCTTTAGA GAAGTTTAAG
    AGGCAATATG GGAAGGTTAA AAATCCTCTG AATACTGAAG TGGAGGAAAA TAATACTTTA
    GAAATCACTA CCAATCTCAG TCCTCAGGTT GAAGCTGACA TTCCATGGAA AGACAAAAAC
    CACTTAGACA ACTCTGACAT CTGTGAAATC ACTACTATGA AATATAATGA TTCCTATGGT
    AGTTGTCAAC CAGTAAATCA CATGTTTTCT TCAGAAAAGC TTCCATTCTC CAAGGAAGAT
    GATTGTTTGG AACAACAGAT GCTTTGCTTA ACAGAAAGCA CTGTAACTCT ACAGGAGTTA
    CCTCATTTTA ACAGTAAATT TTTGGATGTT GAGAAGTCAC CTGAATCACT AGCCTCTAAA
    TTATCCAGAT TGAAGGGTTC CGAAAGAGAG ACTCAAAGGA TGGATGTGAA TCATTTTAAT
    GAATTGCTAC AATTAGATTC CAGTAGGAAA GATGGTGACT TGTCCAGTGG GTTAACCCTA
    GATTCTTGTA AGTTATTTAA AAATGAGCAT AAAAACCCAG AAAGTGACAG TATCCCAATG
    TCAGACTCTG TCACACAAGG TAATCTCTTC AGTAAAGACA GTGAAACATA TTCTAACAAC
    AATACCACTG AGAAGATACC AGAAAATCCT TTGGTACTGC CCTATGACCA TGCTACAGTT
    ATCAGTAAGG ATTCAGATAT TCTTATAGCT TCAAAACAAC AGATTGGAAG TCCTGACTCT
    CTGAGTAGAA TGTTAATGAG TCGCGTGGAA GATTCCACAG CTGACCAAAA TGGAACTTGT
    TTTCAGAGTG AGGAATCCAT AGCAAGAACT TGTTCTGAAA ATGAAGAGTC CAACATATGT
    TCTTTGGATT GGCAGCAGCA TTTTGATGTA ACTCTGGGAA GAATGGTTTA CATCAACAAA
    CTAACAGGAC TTAGCACATT CACTGCTCCT ACTGAGGATG TTCAGGCTGC TTGTACTAAA
    GACCTGACAA CTATGGCTGT GGATGTTCTA CTGGAGAATG GATCTCAGTA CAGGTGTCAT
    CCCTTTAGAA GCGACCTTGT TCTTCCTTTC CTTCCTAGAG CTCGGGAAGA GAGGACTGTG
    ATGAGACAGA ATAACAGAGC TACTGGGGAC GATGCCTTTG GTCCTGAATC ACTTCAATCT
    TTGTTCTCAG AATGGGACAA TCCAGTATTT GCCCGTTATC CAGAGGTTGC TGTTGACGTA
    AGCAGCGGCC AAGCTGAGAG CCTAGCAGTT AAAATTCACA ACATCTTGTA TCCTTATCGT
    TTCACCAAAG AAATGATTCA CTCAATGCAG GTTCTCCAGC AAGTGGATAA CAAGTTTATT
    GCCTGTTTAA TGAGCACTAA GACTGAAGAG AATGGTGAGG CAGGTGGAAA CCTGCTAGTC
    TTGGTGGACC AGCACGCTGC CCACGAGCGC ATCCGATTGG AGCAGCTGAT CATTGATTCC
    TATGAAAAGC AACAGCCACA AGGCTTTGGT CGAAAAAAAT TACTGTCTTC CATTGTAAGT
    CCTCCACTAG AGATAACAGT GACAGAAGAA CAAAGGAGAC TCTTACGGTG TTACCACAAA
    AATCTGGAAG ATCTGGGCCT TGAAATTGTA TTTCCAGACA CCAGTGATTC ACTGGTCCTC
    ATAGGAAAAG TACCACTCTG TTTTGTAGAA AGAGAAGCAA GTGAACTTCG AAGAGGAAGA
    TCTACTGTGA CCAAGGGCAT TGTGGAGGAG TTTATTCGAG AACAAGTGGA GCTACTCCAG
    ACCACAGGAG GCATCCAAGG GACTTTGCCA CTGACTGTCC AGAAGGTGTT GGCATCCCAA
    GCCTGCCATG GGGCCATTAA GTTTAACGAT GGCCTGAGCT TCGAGGAGAG CTGCCGCCTT
    ATTGAAGCGC TGTCCTGGTG CCAGCTGCCG TTCCAGTGTG CTCATGGCAG GCCCTCTATG
    CTGCCATTAG CTAACATCGA CCACTTGGAG CAGGAAAAAC AGACTAAACC CAATCTTGCT
    AAACTTCGCA GAATGGCTCA AGCCTGGCAT CTCTTTGGAA AGGAGAAGGA TGTGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807908.1
    CDS215..4534
    Translation

    Target ORF information:

    RefSeq Version XM_010809606.3
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809606.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGATCAAAT GCTTGTCAGC TGAAGTACAA GCTAGATTGC GTTCTGGTTT GGCTGTATGC 
    TCCTTAGGCC AATGTGTTGA GGAGCTTGCC CTGAACAGTA TTGATGCTGA AGCAAAGTGT
    GTGGCTGTCA GGGTGAACAT GGAAACCTTT CAAGTTCAAG TGATAGACAA TGGATTTGGG
    ATGGGGAGTG ATGATGTAGA CAAGGTGGGA AATCGTTATT TCACCAGTAA ATGCAACTCC
    ATACAGGACT TGGAGAACCC AAGGTTTTAT GGTTTCCGGG GGGAGGCGTT GGCCAGTATA
    GCTGATATGG CCAGTGCTGT GGAAATTTCA TCCAAGAAAA ACAAGTCGAT GAAAACATTT
    GTGAAACTGT TTCAGAATGG GAAAGCCCTG AAAGCTTGTG AAGCTGACTT AACTCGACCA
    AGTGCCGGGA CGACAGTCAC TGTATATAAT CTGTTTTATC AGTTACCTGT TAGGAGGAAA
    TGCATGGATC CTAGACTGGA GTTTGAGAAG GTTAGGCAGA GGATAGAAGC TCTGTCACTC
    ATGCACCCTA CCATTTCTTT CTCTTTGAGA AATGATGTTT CTGGTTCCAT GGTTCTTCAA
    CTCCCTAAAA CCAAAGACAT ATGTTCCCGA TTTTGTCAAA TTTATGGACT GGGTAAGTCC
    CAAAAGTTAA GAGAAATAAA TTTTAAATAT AAGGAGTTTG AGCTTACTGG CTTTATCAGC
    TCCGAAGCAC ATTATAATAA GAATATGCAG TTTTTGTTTG TGAACAAGAG GTTAGTTTTA
    AGGACAAAGT TGCATAAACT CATTGACTTT TTATTAAGGA AAGAAAGTAT TATATGCAAA
    CCAAAGAGTG GTTCTGCCAG TAGGCAAGTG AATTCAAATC CTCGGCCTCG GTCTAACCCG
    GAACTCCATG GTATCTATGT AATCAATGTG CAGTGCCAGT TCTGTGAGTA TGACGTATGC
    CTGGAGCCAG CAAAAACCCT GATTGAGTTT CAGAACTGGG ATACTCTTTT GATTTGCATT
    CAGGAAGGAG TAAAAACGTT TTTAAAGAAA GAAAAATTAT TTGTGGAATT ATCAAGTGAA
    GACATTAAGG AATTTAGTGA AGATAATGAT TTTAGCTTGT TTAGTGCCAG TCTTCAGAAG
    CAAGTGTCCT CTGATGAGAA GTGTGGGCAG GTCAGTTTCC AAGAAGCATG TAATAATATT
    TTGGATTCCT ACGAGATGTT CAATTTGCAG TCAAAAGCTG TGAAAAGAAA AGCTCCTGTA
    GAAAACATAA GCACACAAAA TTCTAGGGAT TCAGAAGCTA TCAGAAAAAA GACAAATGAT
    TCATTTTTGT ACACTTACAA ATCTGATGGG CCAGCCCATT GTAAAATGGC AGAGTCATCT
    TTGCAAAATG AAGACAGTGC TTGCTCACAG TCAAGGAACC TAGAACAAGA GACAGCTGGA
    GCATCAGAAT TGGGAGAAAA TAAGAAACAT AAAAAATCTT GCTTGGAACA TAACTCTTCA
    GAAAATCCAT GTGGAACCAC TTCAGAAATG TTTGGAAGCC CTTTTCAGAC ATTATATCAC
    TTTGAGGGGA GTGGAGAAGA TCTAGAAATA CAGAAAGTAA GTACTACTGT TAATAGCATG
    GCTGCCAACA TCCTGGAAAA TAACAGAATT CAGAATCAAC CAGAGAGATT TAAAGATGCT
    ACTGAAATGG ATTGCCAACC TCTGTCTTTT GAGACAGCAT TATCAGGAGA ACCTAGTGCT
    CAGAGAGAGG AAGAGAAAAG AAAAAAACCT AGTACTTGTG GAAGAATAAA TGTTTTTCGT
    TATGGACAAG TTAAATTATG TTCCACTGGC TTCATAACTC ATGTGGTACA GAATGAGCAA
    ACTAAATTAA CTGAAACAGA ACATTTATTT AAAAGTTATG CTCAACCTGG TCCTGTGAGT
    GCCAAAGAAA CATTTAGAAA TAGAACACAC CATTCAGTTG AGACTCCCAA CAGCAAAGAT
    TTAACCAGCA CTTTAAGTAA AGAATTTGCT GAACTACCCA ACAAAAGTCT GTACAGCACA
    AATCGAAGTT ATGAGCTAGA GAACAAACAT ACAGCAGCCT GTAAAAATTT TGCTATTTTT
    CAGGAAAGTG GTAAAAAATC ACACACAGGT GGCTTATTAC CTAACACATC CTCTTTTTCT
    TGGGGTGGAC ATGTTTCAGA TGGTAGTAAG AAAACAGACA AGTTGATTGG TCCTGCCGAA
    CCCATACCTC ATAAGAAGCT AAGCTTAAGC TCACAACTAG GATCTTTAGA GAAGTTTAAG
    AGGCAATATG GGAAGGTTAA AAATCCTCTG AATACTGAAG TGGAGGAAAA TAATACTTTA
    GAAATCACTA CCAATCTCAG TCCTCAGGTT GAAGCTGACA TTCCATGGAA AGACAAAAAC
    CACTTAGACA ACTCTGACAT CTGTGAAATC ACTACTATGA AATATAATGA TTCCTATGGT
    AGTTGTCAAC CAGTAAATCA CATGTTTTCT TCAGAAAAGC TTCCATTCTC CAAGGAAGAT
    GATTGTTTGG AACAACAGAT GCTTTGCTTA ACAGAAAGCA CTGTAACTCT ACAGGAGTTA
    CCTCATTTTA ACAGTAAATT TTTGGATGTT GAGAAGTCAC CTGAATCACT AGCCTCTAAA
    TTATCCAGAT TGAAGGGTTC CGAAAGAGAG ACTCAAAGGA TGGATGTGAA TCATTTTAAT
    GAATTGCTAC AATTAGATTC CAGTAGGAAA GATGGTGACT TGTCCAGTGG GTTAACCCTA
    GATTCTTGTA AGTTATTTAA AAATGAGCAT AAAAACCCAG AAAGTGACAG TATCCCAATG
    TCAGACTCTG TCACACAAGG TAATCTCTTC AGTAAAGACA GTGAAACATA TTCTAACAAC
    AATACCACTG AGAAGATACC AGAAAATCCT TTGGTACTGC CCTATGACCA TGCTACAGTT
    ATCAGTAAGG ATTCAGATAT TCTTATAGCT TCAAAACAAC AGATTGGAAG TCCTGACTCT
    CTGAGTAGAA TGTTAATGAG TCGCGTGGAA GATTCCACAG CTGACCAAAA TGGAACTTGT
    TTTCAGAGTG AGGAATCCAT AGCAAGAACT TGTTCTGAAA ATGAAGAGTC CAACATATGT
    TCTTTGGATT GGCAGCAGCA TTTTGATGTA ACTCTGGGAA GAATGGTTTA CATCAACAAA
    CTAACAGGAC TTAGCACATT CACTGCTCCT ACTGAGGATG TTCAGGCTGC TTGTACTAAA
    GACCTGACAA CTATGGCTGT GGATGTTCTA CTGGAGAATG GATCTCAGTA CAGGTGTCAT
    CCCTTTAGAA GCGACCTTGT TCTTCCTTTC CTTCCTAGAG CTCGGGAAGA GAGGACTGTG
    ATGAGACAGA ATAACAGAGC TACTGGGGAC GATGCCTTTG GTCCTGAATC ACTTCAATCT
    TTGTTCTCAG AATGGGACAA TCCAGTATTT GCCCGTTATC CAGAGGTTGC TGTTGACGTA
    AGCAGCGGCC AAGCTGAGAG CCTAGCAGTT AAAATTCACA ACATCTTGTA TCCTTATCGT
    TTCACCAAAG AAATGATTCA CTCAATGCAG GTTCTCCAGC AAGTGGATAA CAAGTTTATT
    GCCTGTTTAA TGAGCACTAA GACTGAAGAG AATGGTGAGG CAGGTGGAAA CCTGCTAGTC
    TTGGTGGACC AGCACGCTGC CCACGAGCGC ATCCGATTGG AGCAGCTGAT CATTGATTCC
    TATGAAAAGC AACAGCCACA AGGCTTTGGT CGAAAAAAAT TACTGTCTTC CATTGTAAGT
    CCTCCACTAG AGATAACAGT GACAGAAGAA CAAAGGAGAC TCTTACGGTG TTACCACAAA
    AATCTGGAAG ATCTGGGCCT TGAAATTGTA TTTCCAGACA CCAGTGATTC ACTGGTCCTC
    ATAGGAAAAG TACCACTCTG TTTTGTAGAA AGAGAAGCAA GTGAACTTCG AAGAGGAAGA
    TCTACTGTGA CCAAGGGCAT TGTGGAGGAG TTTATTCGAG AACAAGTGGA GCTACTCCAG
    ACCACAGGAG GCATCCAAGG GACTTTGCCA CTGACTGTCC AGAAGGTGTT GGCATCCCAA
    GCCTGCCATG GGGCCATTAA GTTTAACGAT GGCCTGAGCT TCGAGGAGAG CTGCCGCCTT
    ATTGAAGCGC TGTCCTGGTG CCAGCTGCCG TTCCAGTGTG CTCATGGCAG GCCCTCTATG
    CTGCCATTAG CTAACATCGA CCACTTGGAG CAGGAAAAAC AGACTAAACC CAATCTTGCT
    AAACTTCGCA GAATGGCTCA AGCCTGGCAT CTCTTTGGAA AGGAGAAGGA TGTGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57925
    Clone ID Related Accession (Same CDS sequence) XM_010809611.2 , XM_010809611.3 , XM_010813172.2 , XM_010809606.3 , XM_010813188.2 , XM_010809606.2
    Accession Version XM_010813188.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4320bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813188.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGATCAAAT GCTTGTCAGC TGAAGTACAA GCTAGATTGC GTTCTGGTTT GGCTGTATGC 
    TCCTTAGGCC AATGTGTTGA GGAGCTTGCC CTGAACAGTA TTGATGCTGA AGCAAAGTGT
    GTGGCTGTCA GGGTGAACAT GGAAACCTTT CAAGTTCAAG TGATAGACAA TGGATTTGGG
    ATGGGGAGTG ATGATGTAGA CAAGGTGGGA AATCGTTATT TCACCAGTAA ATGCAACTCC
    ATACAGGACT TGGAGAACCC AAGGTTTTAT GGTTTCCGGG GGGAGGCGTT GGCCAGTATA
    GCTGATATGG CCAGTGCTGT GGAAATTTCA TCCAAGAAAA ACAAGTCGAT GAAAACATTT
    GTGAAACTGT TTCAGAATGG GAAAGCCCTG AAAGCTTGTG AAGCTGACTT AACTCGACCA
    AGTGCCGGGA CGACAGTCAC TGTATATAAT CTGTTTTATC AGTTACCTGT TAGGAGGAAA
    TGCATGGATC CTAGACTGGA GTTTGAGAAG GTTAGGCAGA GGATAGAAGC TCTGTCACTC
    ATGCACCCTA CCATTTCTTT CTCTTTGAGA AATGATGTTT CTGGTTCCAT GGTTCTTCAA
    CTCCCTAAAA CCAAAGACAT ATGTTCCCGA TTTTGTCAAA TTTATGGACT GGGTAAGTCC
    CAAAAGTTAA GAGAAATAAA TTTTAAATAT AAGGAGTTTG AGCTTACTGG CTTTATCAGC
    TCCGAAGCAC ATTATAATAA GAATATGCAG TTTTTGTTTG TGAACAAGAG GTTAGTTTTA
    AGGACAAAGT TGCATAAACT CATTGACTTT TTATTAAGGA AAGAAAGTAT TATATGCAAA
    CCAAAGAGTG GTTCTGCCAG TAGGCAAGTG AATTCAAATC CTCGGCCTCG GTCTAACCCG
    GAACTCCATG GTATCTATGT AATCAATGTG CAGTGCCAGT TCTGTGAGTA TGACGTATGC
    CTGGAGCCAG CAAAAACCCT GATTGAGTTT CAGAACTGGG ATACTCTTTT GATTTGCATT
    CAGGAAGGAG TAAAAACGTT TTTAAAGAAA GAAAAATTAT TTGTGGAATT ATCAAGTGAA
    GACATTAAGG AATTTAGTGA AGATAATGAT TTTAGCTTGT TTAGTGCCAG TCTTCAGAAG
    CAAGTGTCCT CTGATGAGAA GTGTGGGCAG GTCAGTTTCC AAGAAGCATG TAATAATATT
    TTGGATTCCT ACGAGATGTT CAATTTGCAG TCAAAAGCTG TGAAAAGAAA AGCTCCTGTA
    GAAAACATAA GCACACAAAA TTCTAGGGAT TCAGAAGCTA TCAGAAAAAA GACAAATGAT
    TCATTTTTGT ACACTTACAA ATCTGATGGG CCAGCCCATT GTAAAATGGC AGAGTCATCT
    TTGCAAAATG AAGACAGTGC TTGCTCACAG TCAAGGAACC TAGAACAAGA GACAGCTGGA
    GCATCAGAAT TGGGAGAAAA TAAGAAACAT AAAAAATCTT GCTTGGAACA TAACTCTTCA
    GAAAATCCAT GTGGAACCAC TTCAGAAATG TTTGGAAGCC CTTTTCAGAC ATTATATCAC
    TTTGAGGGGA GTGGAGAAGA TCTAGAAATA CAGAAAGTAA GTACTACTGT TAATAGCATG
    GCTGCCAACA TCCTGGAAAA TAACAGAATT CAGAATCAAC CAGAGAGATT TAAAGATGCT
    ACTGAAATGG ATTGCCAACC TCTGTCTTTT GAGACAGCAT TATCAGGAGA ACCTAGTGCT
    CAGAGAGAGG AAGAGAAAAG AAAAAAACCT AGTACTTGTG GAAGAATAAA TGTTTTTCGT
    TATGGACAAG TTAAATTATG TTCCACTGGC TTCATAACTC ATGTGGTACA GAATGAGCAA
    ACTAAATTAA CTGAAACAGA ACATTTATTT AAAAGTTATG CTCAACCTGG TCCTGTGAGT
    GCCAAAGAAA CATTTAGAAA TAGAACACAC CATTCAGTTG AGACTCCCAA CAGCAAAGAT
    TTAACCAGCA CTTTAAGTAA AGAATTTGCT GAACTACCCA ACAAAAGTCT GTACAGCACA
    AATCGAAGTT ATGAGCTAGA GAACAAACAT ACAGCAGCCT GTAAAAATTT TGCTATTTTT
    CAGGAAAGTG GTAAAAAATC ACACACAGGT GGCTTATTAC CTAACACATC CTCTTTTTCT
    TGGGGTGGAC ATGTTTCAGA TGGTAGTAAG AAAACAGACA AGTTGATTGG TCCTGCCGAA
    CCCATACCTC ATAAGAAGCT AAGCTTAAGC TCACAACTAG GATCTTTAGA GAAGTTTAAG
    AGGCAATATG GGAAGGTTAA AAATCCTCTG AATACTGAAG TGGAGGAAAA TAATACTTTA
    GAAATCACTA CCAATCTCAG TCCTCAGGTT GAAGCTGACA TTCCATGGAA AGACAAAAAC
    CACTTAGACA ACTCTGACAT CTGTGAAATC ACTACTATGA AATATAATGA TTCCTATGGT
    AGTTGTCAAC CAGTAAATCA CATGTTTTCT TCAGAAAAGC TTCCATTCTC CAAGGAAGAT
    GATTGTTTGG AACAACAGAT GCTTTGCTTA ACAGAAAGCA CTGTAACTCT ACAGGAGTTA
    CCTCATTTTA ACAGTAAATT TTTGGATGTT GAGAAGTCAC CTGAATCACT AGCCTCTAAA
    TTATCCAGAT TGAAGGGTTC CGAAAGAGAG ACTCAAAGGA TGGATGTGAA TCATTTTAAT
    GAATTGCTAC AATTAGATTC CAGTAGGAAA GATGGTGACT TGTCCAGTGG GTTAACCCTA
    GATTCTTGTA AGTTATTTAA AAATGAGCAT AAAAACCCAG AAAGTGACAG TATCCCAATG
    TCAGACTCTG TCACACAAGG TAATCTCTTC AGTAAAGACA GTGAAACATA TTCTAACAAC
    AATACCACTG AGAAGATACC AGAAAATCCT TTGGTACTGC CCTATGACCA TGCTACAGTT
    ATCAGTAAGG ATTCAGATAT TCTTATAGCT TCAAAACAAC AGATTGGAAG TCCTGACTCT
    CTGAGTAGAA TGTTAATGAG TCGCGTGGAA GATTCCACAG CTGACCAAAA TGGAACTTGT
    TTTCAGAGTG AGGAATCCAT AGCAAGAACT TGTTCTGAAA ATGAAGAGTC CAACATATGT
    TCTTTGGATT GGCAGCAGCA TTTTGATGTA ACTCTGGGAA GAATGGTTTA CATCAACAAA
    CTAACAGGAC TTAGCACATT CACTGCTCCT ACTGAGGATG TTCAGGCTGC TTGTACTAAA
    GACCTGACAA CTATGGCTGT GGATGTTCTA CTGGAGAATG GATCTCAGTA CAGGTGTCAT
    CCCTTTAGAA GCGACCTTGT TCTTCCTTTC CTTCCTAGAG CTCGGGAAGA GAGGACTGTG
    ATGAGACAGA ATAACAGAGC TACTGGGGAC GATGCCTTTG GTCCTGAATC ACTTCAATCT
    TTGTTCTCAG AATGGGACAA TCCAGTATTT GCCCGTTATC CAGAGGTTGC TGTTGACGTA
    AGCAGCGGCC AAGCTGAGAG CCTAGCAGTT AAAATTCACA ACATCTTGTA TCCTTATCGT
    TTCACCAAAG AAATGATTCA CTCAATGCAG GTTCTCCAGC AAGTGGATAA CAAGTTTATT
    GCCTGTTTAA TGAGCACTAA GACTGAAGAG AATGGTGAGG CAGGTGGAAA CCTGCTAGTC
    TTGGTGGACC AGCACGCTGC CCACGAGCGC ATCCGATTGG AGCAGCTGAT CATTGATTCC
    TATGAAAAGC AACAGCCACA AGGCTTTGGT CGAAAAAAAT TACTGTCTTC CATTGTAAGT
    CCTCCACTAG AGATAACAGT GACAGAAGAA CAAAGGAGAC TCTTACGGTG TTACCACAAA
    AATCTGGAAG ATCTGGGCCT TGAAATTGTA TTTCCAGACA CCAGTGATTC ACTGGTCCTC
    ATAGGAAAAG TACCACTCTG TTTTGTAGAA AGAGAAGCAA GTGAACTTCG AAGAGGAAGA
    TCTACTGTGA CCAAGGGCAT TGTGGAGGAG TTTATTCGAG AACAAGTGGA GCTACTCCAG
    ACCACAGGAG GCATCCAAGG GACTTTGCCA CTGACTGTCC AGAAGGTGTT GGCATCCCAA
    GCCTGCCATG GGGCCATTAA GTTTAACGAT GGCCTGAGCT TCGAGGAGAG CTGCCGCCTT
    ATTGAAGCGC TGTCCTGGTG CCAGCTGCCG TTCCAGTGTG CTCATGGCAG GCCCTCTATG
    CTGCCATTAG CTAACATCGA CCACTTGGAG CAGGAAAAAC AGACTAAACC CAATCTTGCT
    AAACTTCGCA GAATGGCTCA AGCCTGGCAT CTCTTTGGAA AGGAGAAGGA TGTGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811490.1
    CDS229..4548
    Translation

    Target ORF information:

    RefSeq Version XM_010813188.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813188.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGATCAAAT GCTTGTCAGC TGAAGTACAA GCTAGATTGC GTTCTGGTTT GGCTGTATGC 
    TCCTTAGGCC AATGTGTTGA GGAGCTTGCC CTGAACAGTA TTGATGCTGA AGCAAAGTGT
    GTGGCTGTCA GGGTGAACAT GGAAACCTTT CAAGTTCAAG TGATAGACAA TGGATTTGGG
    ATGGGGAGTG ATGATGTAGA CAAGGTGGGA AATCGTTATT TCACCAGTAA ATGCAACTCC
    ATACAGGACT TGGAGAACCC AAGGTTTTAT GGTTTCCGGG GGGAGGCGTT GGCCAGTATA
    GCTGATATGG CCAGTGCTGT GGAAATTTCA TCCAAGAAAA ACAAGTCGAT GAAAACATTT
    GTGAAACTGT TTCAGAATGG GAAAGCCCTG AAAGCTTGTG AAGCTGACTT AACTCGACCA
    AGTGCCGGGA CGACAGTCAC TGTATATAAT CTGTTTTATC AGTTACCTGT TAGGAGGAAA
    TGCATGGATC CTAGACTGGA GTTTGAGAAG GTTAGGCAGA GGATAGAAGC TCTGTCACTC
    ATGCACCCTA CCATTTCTTT CTCTTTGAGA AATGATGTTT CTGGTTCCAT GGTTCTTCAA
    CTCCCTAAAA CCAAAGACAT ATGTTCCCGA TTTTGTCAAA TTTATGGACT GGGTAAGTCC
    CAAAAGTTAA GAGAAATAAA TTTTAAATAT AAGGAGTTTG AGCTTACTGG CTTTATCAGC
    TCCGAAGCAC ATTATAATAA GAATATGCAG TTTTTGTTTG TGAACAAGAG GTTAGTTTTA
    AGGACAAAGT TGCATAAACT CATTGACTTT TTATTAAGGA AAGAAAGTAT TATATGCAAA
    CCAAAGAGTG GTTCTGCCAG TAGGCAAGTG AATTCAAATC CTCGGCCTCG GTCTAACCCG
    GAACTCCATG GTATCTATGT AATCAATGTG CAGTGCCAGT TCTGTGAGTA TGACGTATGC
    CTGGAGCCAG CAAAAACCCT GATTGAGTTT CAGAACTGGG ATACTCTTTT GATTTGCATT
    CAGGAAGGAG TAAAAACGTT TTTAAAGAAA GAAAAATTAT TTGTGGAATT ATCAAGTGAA
    GACATTAAGG AATTTAGTGA AGATAATGAT TTTAGCTTGT TTAGTGCCAG TCTTCAGAAG
    CAAGTGTCCT CTGATGAGAA GTGTGGGCAG GTCAGTTTCC AAGAAGCATG TAATAATATT
    TTGGATTCCT ACGAGATGTT CAATTTGCAG TCAAAAGCTG TGAAAAGAAA AGCTCCTGTA
    GAAAACATAA GCACACAAAA TTCTAGGGAT TCAGAAGCTA TCAGAAAAAA GACAAATGAT
    TCATTTTTGT ACACTTACAA ATCTGATGGG CCAGCCCATT GTAAAATGGC AGAGTCATCT
    TTGCAAAATG AAGACAGTGC TTGCTCACAG TCAAGGAACC TAGAACAAGA GACAGCTGGA
    GCATCAGAAT TGGGAGAAAA TAAGAAACAT AAAAAATCTT GCTTGGAACA TAACTCTTCA
    GAAAATCCAT GTGGAACCAC TTCAGAAATG TTTGGAAGCC CTTTTCAGAC ATTATATCAC
    TTTGAGGGGA GTGGAGAAGA TCTAGAAATA CAGAAAGTAA GTACTACTGT TAATAGCATG
    GCTGCCAACA TCCTGGAAAA TAACAGAATT CAGAATCAAC CAGAGAGATT TAAAGATGCT
    ACTGAAATGG ATTGCCAACC TCTGTCTTTT GAGACAGCAT TATCAGGAGA ACCTAGTGCT
    CAGAGAGAGG AAGAGAAAAG AAAAAAACCT AGTACTTGTG GAAGAATAAA TGTTTTTCGT
    TATGGACAAG TTAAATTATG TTCCACTGGC TTCATAACTC ATGTGGTACA GAATGAGCAA
    ACTAAATTAA CTGAAACAGA ACATTTATTT AAAAGTTATG CTCAACCTGG TCCTGTGAGT
    GCCAAAGAAA CATTTAGAAA TAGAACACAC CATTCAGTTG AGACTCCCAA CAGCAAAGAT
    TTAACCAGCA CTTTAAGTAA AGAATTTGCT GAACTACCCA ACAAAAGTCT GTACAGCACA
    AATCGAAGTT ATGAGCTAGA GAACAAACAT ACAGCAGCCT GTAAAAATTT TGCTATTTTT
    CAGGAAAGTG GTAAAAAATC ACACACAGGT GGCTTATTAC CTAACACATC CTCTTTTTCT
    TGGGGTGGAC ATGTTTCAGA TGGTAGTAAG AAAACAGACA AGTTGATTGG TCCTGCCGAA
    CCCATACCTC ATAAGAAGCT AAGCTTAAGC TCACAACTAG GATCTTTAGA GAAGTTTAAG
    AGGCAATATG GGAAGGTTAA AAATCCTCTG AATACTGAAG TGGAGGAAAA TAATACTTTA
    GAAATCACTA CCAATCTCAG TCCTCAGGTT GAAGCTGACA TTCCATGGAA AGACAAAAAC
    CACTTAGACA ACTCTGACAT CTGTGAAATC ACTACTATGA AATATAATGA TTCCTATGGT
    AGTTGTCAAC CAGTAAATCA CATGTTTTCT TCAGAAAAGC TTCCATTCTC CAAGGAAGAT
    GATTGTTTGG AACAACAGAT GCTTTGCTTA ACAGAAAGCA CTGTAACTCT ACAGGAGTTA
    CCTCATTTTA ACAGTAAATT TTTGGATGTT GAGAAGTCAC CTGAATCACT AGCCTCTAAA
    TTATCCAGAT TGAAGGGTTC CGAAAGAGAG ACTCAAAGGA TGGATGTGAA TCATTTTAAT
    GAATTGCTAC AATTAGATTC CAGTAGGAAA GATGGTGACT TGTCCAGTGG GTTAACCCTA
    GATTCTTGTA AGTTATTTAA AAATGAGCAT AAAAACCCAG AAAGTGACAG TATCCCAATG
    TCAGACTCTG TCACACAAGG TAATCTCTTC AGTAAAGACA GTGAAACATA TTCTAACAAC
    AATACCACTG AGAAGATACC AGAAAATCCT TTGGTACTGC CCTATGACCA TGCTACAGTT
    ATCAGTAAGG ATTCAGATAT TCTTATAGCT TCAAAACAAC AGATTGGAAG TCCTGACTCT
    CTGAGTAGAA TGTTAATGAG TCGCGTGGAA GATTCCACAG CTGACCAAAA TGGAACTTGT
    TTTCAGAGTG AGGAATCCAT AGCAAGAACT TGTTCTGAAA ATGAAGAGTC CAACATATGT
    TCTTTGGATT GGCAGCAGCA TTTTGATGTA ACTCTGGGAA GAATGGTTTA CATCAACAAA
    CTAACAGGAC TTAGCACATT CACTGCTCCT ACTGAGGATG TTCAGGCTGC TTGTACTAAA
    GACCTGACAA CTATGGCTGT GGATGTTCTA CTGGAGAATG GATCTCAGTA CAGGTGTCAT
    CCCTTTAGAA GCGACCTTGT TCTTCCTTTC CTTCCTAGAG CTCGGGAAGA GAGGACTGTG
    ATGAGACAGA ATAACAGAGC TACTGGGGAC GATGCCTTTG GTCCTGAATC ACTTCAATCT
    TTGTTCTCAG AATGGGACAA TCCAGTATTT GCCCGTTATC CAGAGGTTGC TGTTGACGTA
    AGCAGCGGCC AAGCTGAGAG CCTAGCAGTT AAAATTCACA ACATCTTGTA TCCTTATCGT
    TTCACCAAAG AAATGATTCA CTCAATGCAG GTTCTCCAGC AAGTGGATAA CAAGTTTATT
    GCCTGTTTAA TGAGCACTAA GACTGAAGAG AATGGTGAGG CAGGTGGAAA CCTGCTAGTC
    TTGGTGGACC AGCACGCTGC CCACGAGCGC ATCCGATTGG AGCAGCTGAT CATTGATTCC
    TATGAAAAGC AACAGCCACA AGGCTTTGGT CGAAAAAAAT TACTGTCTTC CATTGTAAGT
    CCTCCACTAG AGATAACAGT GACAGAAGAA CAAAGGAGAC TCTTACGGTG TTACCACAAA
    AATCTGGAAG ATCTGGGCCT TGAAATTGTA TTTCCAGACA CCAGTGATTC ACTGGTCCTC
    ATAGGAAAAG TACCACTCTG TTTTGTAGAA AGAGAAGCAA GTGAACTTCG AAGAGGAAGA
    TCTACTGTGA CCAAGGGCAT TGTGGAGGAG TTTATTCGAG AACAAGTGGA GCTACTCCAG
    ACCACAGGAG GCATCCAAGG GACTTTGCCA CTGACTGTCC AGAAGGTGTT GGCATCCCAA
    GCCTGCCATG GGGCCATTAA GTTTAACGAT GGCCTGAGCT TCGAGGAGAG CTGCCGCCTT
    ATTGAAGCGC TGTCCTGGTG CCAGCTGCCG TTCCAGTGTG CTCATGGCAG GCCCTCTATG
    CTGCCATTAG CTAACATCGA CCACTTGGAG CAGGAAAAAC AGACTAAACC CAATCTTGCT
    AAACTTCGCA GAATGGCTCA AGCCTGGCAT CTCTTTGGAA AGGAGAAGGA TGTGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57925
    Clone ID Related Accession (Same CDS sequence) XM_010809611.2 , XM_010809611.3 , XM_010813172.2 , XM_010809606.3 , XM_010813188.2 , XM_010809606.2
    Accession Version XM_010809606.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4320bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104283.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809606.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGATCAAAT GCTTGTCAGC TGAAGTACAA GCTAGATTGC GTTCTGGTTT GGCTGTATGC 
    TCCTTAGGCC AATGTGTTGA GGAGCTTGCC CTGAACAGTA TTGATGCTGA AGCAAAGTGT
    GTGGCTGTCA GGGTGAACAT GGAAACCTTT CAAGTTCAAG TGATAGACAA TGGATTTGGG
    ATGGGGAGTG ATGATGTAGA CAAGGTGGGA AATCGTTATT TCACCAGTAA ATGCAACTCC
    ATACAGGACT TGGAGAACCC AAGGTTTTAT GGTTTCCGGG GGGAGGCGTT GGCCAGTATA
    GCTGATATGG CCAGTGCTGT GGAAATTTCA TCCAAGAAAA ACAAGTCGAT GAAAACATTT
    GTGAAACTGT TTCAGAATGG GAAAGCCCTG AAAGCTTGTG AAGCTGACTT AACTCGACCA
    AGTGCCGGGA CGACAGTCAC TGTATATAAT CTGTTTTATC AGTTACCTGT TAGGAGGAAA
    TGCATGGATC CTAGACTGGA GTTTGAGAAG GTTAGGCAGA GGATAGAAGC TCTGTCACTC
    ATGCACCCTA CCATTTCTTT CTCTTTGAGA AATGATGTTT CTGGTTCCAT GGTTCTTCAA
    CTCCCTAAAA CCAAAGACAT ATGTTCCCGA TTTTGTCAAA TTTATGGACT GGGTAAGTCC
    CAAAAGTTAA GAGAAATAAA TTTTAAATAT AAGGAGTTTG AGCTTACTGG CTTTATCAGC
    TCCGAAGCAC ATTATAATAA GAATATGCAG TTTTTGTTTG TGAACAAGAG GTTAGTTTTA
    AGGACAAAGT TGCATAAACT CATTGACTTT TTATTAAGGA AAGAAAGTAT TATATGCAAA
    CCAAAGAGTG GTTCTGCCAG TAGGCAAGTG AATTCAAATC CTCGGCCTCG GTCTAACCCG
    GAACTCCATG GTATCTATGT AATCAATGTG CAGTGCCAGT TCTGTGAGTA TGACGTATGC
    CTGGAGCCAG CAAAAACCCT GATTGAGTTT CAGAACTGGG ATACTCTTTT GATTTGCATT
    CAGGAAGGAG TAAAAACGTT TTTAAAGAAA GAAAAATTAT TTGTGGAATT ATCAAGTGAA
    GACATTAAGG AATTTAGTGA AGATAATGAT TTTAGCTTGT TTAGTGCCAG TCTTCAGAAG
    CAAGTGTCCT CTGATGAGAA GTGTGGGCAG GTCAGTTTCC AAGAAGCATG TAATAATATT
    TTGGATTCCT ACGAGATGTT CAATTTGCAG TCAAAAGCTG TGAAAAGAAA AGCTCCTGTA
    GAAAACATAA GCACACAAAA TTCTAGGGAT TCAGAAGCTA TCAGAAAAAA GACAAATGAT
    TCATTTTTGT ACACTTACAA ATCTGATGGG CCAGCCCATT GTAAAATGGC AGAGTCATCT
    TTGCAAAATG AAGACAGTGC TTGCTCACAG TCAAGGAACC TAGAACAAGA GACAGCTGGA
    GCATCAGAAT TGGGAGAAAA TAAGAAACAT AAAAAATCTT GCTTGGAACA TAACTCTTCA
    GAAAATCCAT GTGGAACCAC TTCAGAAATG TTTGGAAGCC CTTTTCAGAC ATTATATCAC
    TTTGAGGGGA GTGGAGAAGA TCTAGAAATA CAGAAAGTAA GTACTACTGT TAATAGCATG
    GCTGCCAACA TCCTGGAAAA TAACAGAATT CAGAATCAAC CAGAGAGATT TAAAGATGCT
    ACTGAAATGG ATTGCCAACC TCTGTCTTTT GAGACAGCAT TATCAGGAGA ACCTAGTGCT
    CAGAGAGAGG AAGAGAAAAG AAAAAAACCT AGTACTTGTG GAAGAATAAA TGTTTTTCGT
    TATGGACAAG TTAAATTATG TTCCACTGGC TTCATAACTC ATGTGGTACA GAATGAGCAA
    ACTAAATTAA CTGAAACAGA ACATTTATTT AAAAGTTATG CTCAACCTGG TCCTGTGAGT
    GCCAAAGAAA CATTTAGAAA TAGAACACAC CATTCAGTTG AGACTCCCAA CAGCAAAGAT
    TTAACCAGCA CTTTAAGTAA AGAATTTGCT GAACTACCCA ACAAAAGTCT GTACAGCACA
    AATCGAAGTT ATGAGCTAGA GAACAAACAT ACAGCAGCCT GTAAAAATTT TGCTATTTTT
    CAGGAAAGTG GTAAAAAATC ACACACAGGT GGCTTATTAC CTAACACATC CTCTTTTTCT
    TGGGGTGGAC ATGTTTCAGA TGGTAGTAAG AAAACAGACA AGTTGATTGG TCCTGCCGAA
    CCCATACCTC ATAAGAAGCT AAGCTTAAGC TCACAACTAG GATCTTTAGA GAAGTTTAAG
    AGGCAATATG GGAAGGTTAA AAATCCTCTG AATACTGAAG TGGAGGAAAA TAATACTTTA
    GAAATCACTA CCAATCTCAG TCCTCAGGTT GAAGCTGACA TTCCATGGAA AGACAAAAAC
    CACTTAGACA ACTCTGACAT CTGTGAAATC ACTACTATGA AATATAATGA TTCCTATGGT
    AGTTGTCAAC CAGTAAATCA CATGTTTTCT TCAGAAAAGC TTCCATTCTC CAAGGAAGAT
    GATTGTTTGG AACAACAGAT GCTTTGCTTA ACAGAAAGCA CTGTAACTCT ACAGGAGTTA
    CCTCATTTTA ACAGTAAATT TTTGGATGTT GAGAAGTCAC CTGAATCACT AGCCTCTAAA
    TTATCCAGAT TGAAGGGTTC CGAAAGAGAG ACTCAAAGGA TGGATGTGAA TCATTTTAAT
    GAATTGCTAC AATTAGATTC CAGTAGGAAA GATGGTGACT TGTCCAGTGG GTTAACCCTA
    GATTCTTGTA AGTTATTTAA AAATGAGCAT AAAAACCCAG AAAGTGACAG TATCCCAATG
    TCAGACTCTG TCACACAAGG TAATCTCTTC AGTAAAGACA GTGAAACATA TTCTAACAAC
    AATACCACTG AGAAGATACC AGAAAATCCT TTGGTACTGC CCTATGACCA TGCTACAGTT
    ATCAGTAAGG ATTCAGATAT TCTTATAGCT TCAAAACAAC AGATTGGAAG TCCTGACTCT
    CTGAGTAGAA TGTTAATGAG TCGCGTGGAA GATTCCACAG CTGACCAAAA TGGAACTTGT
    TTTCAGAGTG AGGAATCCAT AGCAAGAACT TGTTCTGAAA ATGAAGAGTC CAACATATGT
    TCTTTGGATT GGCAGCAGCA TTTTGATGTA ACTCTGGGAA GAATGGTTTA CATCAACAAA
    CTAACAGGAC TTAGCACATT CACTGCTCCT ACTGAGGATG TTCAGGCTGC TTGTACTAAA
    GACCTGACAA CTATGGCTGT GGATGTTCTA CTGGAGAATG GATCTCAGTA CAGGTGTCAT
    CCCTTTAGAA GCGACCTTGT TCTTCCTTTC CTTCCTAGAG CTCGGGAAGA GAGGACTGTG
    ATGAGACAGA ATAACAGAGC TACTGGGGAC GATGCCTTTG GTCCTGAATC ACTTCAATCT
    TTGTTCTCAG AATGGGACAA TCCAGTATTT GCCCGTTATC CAGAGGTTGC TGTTGACGTA
    AGCAGCGGCC AAGCTGAGAG CCTAGCAGTT AAAATTCACA ACATCTTGTA TCCTTATCGT
    TTCACCAAAG AAATGATTCA CTCAATGCAG GTTCTCCAGC AAGTGGATAA CAAGTTTATT
    GCCTGTTTAA TGAGCACTAA GACTGAAGAG AATGGTGAGG CAGGTGGAAA CCTGCTAGTC
    TTGGTGGACC AGCACGCTGC CCACGAGCGC ATCCGATTGG AGCAGCTGAT CATTGATTCC
    TATGAAAAGC AACAGCCACA AGGCTTTGGT CGAAAAAAAT TACTGTCTTC CATTGTAAGT
    CCTCCACTAG AGATAACAGT GACAGAAGAA CAAAGGAGAC TCTTACGGTG TTACCACAAA
    AATCTGGAAG ATCTGGGCCT TGAAATTGTA TTTCCAGACA CCAGTGATTC ACTGGTCCTC
    ATAGGAAAAG TACCACTCTG TTTTGTAGAA AGAGAAGCAA GTGAACTTCG AAGAGGAAGA
    TCTACTGTGA CCAAGGGCAT TGTGGAGGAG TTTATTCGAG AACAAGTGGA GCTACTCCAG
    ACCACAGGAG GCATCCAAGG GACTTTGCCA CTGACTGTCC AGAAGGTGTT GGCATCCCAA
    GCCTGCCATG GGGCCATTAA GTTTAACGAT GGCCTGAGCT TCGAGGAGAG CTGCCGCCTT
    ATTGAAGCGC TGTCCTGGTG CCAGCTGCCG TTCCAGTGTG CTCATGGCAG GCCCTCTATG
    CTGCCATTAG CTAACATCGA CCACTTGGAG CAGGAAAAAC AGACTAAACC CAATCTTGCT
    AAACTTCGCA GAATGGCTCA AGCCTGGCAT CTCTTTGGAA AGGAGAAGGA TGTGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807908.1
    CDS185..4504
    Translation

    Target ORF information:

    RefSeq Version XM_010809606.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809606.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGATCAAAT GCTTGTCAGC TGAAGTACAA GCTAGATTGC GTTCTGGTTT GGCTGTATGC 
    TCCTTAGGCC AATGTGTTGA GGAGCTTGCC CTGAACAGTA TTGATGCTGA AGCAAAGTGT
    GTGGCTGTCA GGGTGAACAT GGAAACCTTT CAAGTTCAAG TGATAGACAA TGGATTTGGG
    ATGGGGAGTG ATGATGTAGA CAAGGTGGGA AATCGTTATT TCACCAGTAA ATGCAACTCC
    ATACAGGACT TGGAGAACCC AAGGTTTTAT GGTTTCCGGG GGGAGGCGTT GGCCAGTATA
    GCTGATATGG CCAGTGCTGT GGAAATTTCA TCCAAGAAAA ACAAGTCGAT GAAAACATTT
    GTGAAACTGT TTCAGAATGG GAAAGCCCTG AAAGCTTGTG AAGCTGACTT AACTCGACCA
    AGTGCCGGGA CGACAGTCAC TGTATATAAT CTGTTTTATC AGTTACCTGT TAGGAGGAAA
    TGCATGGATC CTAGACTGGA GTTTGAGAAG GTTAGGCAGA GGATAGAAGC TCTGTCACTC
    ATGCACCCTA CCATTTCTTT CTCTTTGAGA AATGATGTTT CTGGTTCCAT GGTTCTTCAA
    CTCCCTAAAA CCAAAGACAT ATGTTCCCGA TTTTGTCAAA TTTATGGACT GGGTAAGTCC
    CAAAAGTTAA GAGAAATAAA TTTTAAATAT AAGGAGTTTG AGCTTACTGG CTTTATCAGC
    TCCGAAGCAC ATTATAATAA GAATATGCAG TTTTTGTTTG TGAACAAGAG GTTAGTTTTA
    AGGACAAAGT TGCATAAACT CATTGACTTT TTATTAAGGA AAGAAAGTAT TATATGCAAA
    CCAAAGAGTG GTTCTGCCAG TAGGCAAGTG AATTCAAATC CTCGGCCTCG GTCTAACCCG
    GAACTCCATG GTATCTATGT AATCAATGTG CAGTGCCAGT TCTGTGAGTA TGACGTATGC
    CTGGAGCCAG CAAAAACCCT GATTGAGTTT CAGAACTGGG ATACTCTTTT GATTTGCATT
    CAGGAAGGAG TAAAAACGTT TTTAAAGAAA GAAAAATTAT TTGTGGAATT ATCAAGTGAA
    GACATTAAGG AATTTAGTGA AGATAATGAT TTTAGCTTGT TTAGTGCCAG TCTTCAGAAG
    CAAGTGTCCT CTGATGAGAA GTGTGGGCAG GTCAGTTTCC AAGAAGCATG TAATAATATT
    TTGGATTCCT ACGAGATGTT CAATTTGCAG TCAAAAGCTG TGAAAAGAAA AGCTCCTGTA
    GAAAACATAA GCACACAAAA TTCTAGGGAT TCAGAAGCTA TCAGAAAAAA GACAAATGAT
    TCATTTTTGT ACACTTACAA ATCTGATGGG CCAGCCCATT GTAAAATGGC AGAGTCATCT
    TTGCAAAATG AAGACAGTGC TTGCTCACAG TCAAGGAACC TAGAACAAGA GACAGCTGGA
    GCATCAGAAT TGGGAGAAAA TAAGAAACAT AAAAAATCTT GCTTGGAACA TAACTCTTCA
    GAAAATCCAT GTGGAACCAC TTCAGAAATG TTTGGAAGCC CTTTTCAGAC ATTATATCAC
    TTTGAGGGGA GTGGAGAAGA TCTAGAAATA CAGAAAGTAA GTACTACTGT TAATAGCATG
    GCTGCCAACA TCCTGGAAAA TAACAGAATT CAGAATCAAC CAGAGAGATT TAAAGATGCT
    ACTGAAATGG ATTGCCAACC TCTGTCTTTT GAGACAGCAT TATCAGGAGA ACCTAGTGCT
    CAGAGAGAGG AAGAGAAAAG AAAAAAACCT AGTACTTGTG GAAGAATAAA TGTTTTTCGT
    TATGGACAAG TTAAATTATG TTCCACTGGC TTCATAACTC ATGTGGTACA GAATGAGCAA
    ACTAAATTAA CTGAAACAGA ACATTTATTT AAAAGTTATG CTCAACCTGG TCCTGTGAGT
    GCCAAAGAAA CATTTAGAAA TAGAACACAC CATTCAGTTG AGACTCCCAA CAGCAAAGAT
    TTAACCAGCA CTTTAAGTAA AGAATTTGCT GAACTACCCA ACAAAAGTCT GTACAGCACA
    AATCGAAGTT ATGAGCTAGA GAACAAACAT ACAGCAGCCT GTAAAAATTT TGCTATTTTT
    CAGGAAAGTG GTAAAAAATC ACACACAGGT GGCTTATTAC CTAACACATC CTCTTTTTCT
    TGGGGTGGAC ATGTTTCAGA TGGTAGTAAG AAAACAGACA AGTTGATTGG TCCTGCCGAA
    CCCATACCTC ATAAGAAGCT AAGCTTAAGC TCACAACTAG GATCTTTAGA GAAGTTTAAG
    AGGCAATATG GGAAGGTTAA AAATCCTCTG AATACTGAAG TGGAGGAAAA TAATACTTTA
    GAAATCACTA CCAATCTCAG TCCTCAGGTT GAAGCTGACA TTCCATGGAA AGACAAAAAC
    CACTTAGACA ACTCTGACAT CTGTGAAATC ACTACTATGA AATATAATGA TTCCTATGGT
    AGTTGTCAAC CAGTAAATCA CATGTTTTCT TCAGAAAAGC TTCCATTCTC CAAGGAAGAT
    GATTGTTTGG AACAACAGAT GCTTTGCTTA ACAGAAAGCA CTGTAACTCT ACAGGAGTTA
    CCTCATTTTA ACAGTAAATT TTTGGATGTT GAGAAGTCAC CTGAATCACT AGCCTCTAAA
    TTATCCAGAT TGAAGGGTTC CGAAAGAGAG ACTCAAAGGA TGGATGTGAA TCATTTTAAT
    GAATTGCTAC AATTAGATTC CAGTAGGAAA GATGGTGACT TGTCCAGTGG GTTAACCCTA
    GATTCTTGTA AGTTATTTAA AAATGAGCAT AAAAACCCAG AAAGTGACAG TATCCCAATG
    TCAGACTCTG TCACACAAGG TAATCTCTTC AGTAAAGACA GTGAAACATA TTCTAACAAC
    AATACCACTG AGAAGATACC AGAAAATCCT TTGGTACTGC CCTATGACCA TGCTACAGTT
    ATCAGTAAGG ATTCAGATAT TCTTATAGCT TCAAAACAAC AGATTGGAAG TCCTGACTCT
    CTGAGTAGAA TGTTAATGAG TCGCGTGGAA GATTCCACAG CTGACCAAAA TGGAACTTGT
    TTTCAGAGTG AGGAATCCAT AGCAAGAACT TGTTCTGAAA ATGAAGAGTC CAACATATGT
    TCTTTGGATT GGCAGCAGCA TTTTGATGTA ACTCTGGGAA GAATGGTTTA CATCAACAAA
    CTAACAGGAC TTAGCACATT CACTGCTCCT ACTGAGGATG TTCAGGCTGC TTGTACTAAA
    GACCTGACAA CTATGGCTGT GGATGTTCTA CTGGAGAATG GATCTCAGTA CAGGTGTCAT
    CCCTTTAGAA GCGACCTTGT TCTTCCTTTC CTTCCTAGAG CTCGGGAAGA GAGGACTGTG
    ATGAGACAGA ATAACAGAGC TACTGGGGAC GATGCCTTTG GTCCTGAATC ACTTCAATCT
    TTGTTCTCAG AATGGGACAA TCCAGTATTT GCCCGTTATC CAGAGGTTGC TGTTGACGTA
    AGCAGCGGCC AAGCTGAGAG CCTAGCAGTT AAAATTCACA ACATCTTGTA TCCTTATCGT
    TTCACCAAAG AAATGATTCA CTCAATGCAG GTTCTCCAGC AAGTGGATAA CAAGTTTATT
    GCCTGTTTAA TGAGCACTAA GACTGAAGAG AATGGTGAGG CAGGTGGAAA CCTGCTAGTC
    TTGGTGGACC AGCACGCTGC CCACGAGCGC ATCCGATTGG AGCAGCTGAT CATTGATTCC
    TATGAAAAGC AACAGCCACA AGGCTTTGGT CGAAAAAAAT TACTGTCTTC CATTGTAAGT
    CCTCCACTAG AGATAACAGT GACAGAAGAA CAAAGGAGAC TCTTACGGTG TTACCACAAA
    AATCTGGAAG ATCTGGGCCT TGAAATTGTA TTTCCAGACA CCAGTGATTC ACTGGTCCTC
    ATAGGAAAAG TACCACTCTG TTTTGTAGAA AGAGAAGCAA GTGAACTTCG AAGAGGAAGA
    TCTACTGTGA CCAAGGGCAT TGTGGAGGAG TTTATTCGAG AACAAGTGGA GCTACTCCAG
    ACCACAGGAG GCATCCAAGG GACTTTGCCA CTGACTGTCC AGAAGGTGTT GGCATCCCAA
    GCCTGCCATG GGGCCATTAA GTTTAACGAT GGCCTGAGCT TCGAGGAGAG CTGCCGCCTT
    ATTGAAGCGC TGTCCTGGTG CCAGCTGCCG TTCCAGTGTG CTCATGGCAG GCCCTCTATG
    CTGCCATTAG CTAACATCGA CCACTTGGAG CAGGAAAAAC AGACTAAACC CAATCTTGCT
    AAACTTCGCA GAATGGCTCA AGCCTGGCAT CTCTTTGGAA AGGAGAAGGA TGTGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57926
    Clone ID Related Accession (Same CDS sequence) XM_010809607.2 , XM_010813177.2 , XM_010809607.3
    Accession Version XM_010809607.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104283.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809607.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGTAA GTCCCAAAAG TTAAGAGAAA TAAATTTTAA ATATAAGGAG
    TTTGAGCTTA CTGGCTTTAT CAGCTCCGAA GCACATTATA ATAAGAATAT GCAGTTTTTG
    TTTGTGAACA AGAGGTTAGT TTTAAGGACA AAGTTGCATA AACTCATTGA CTTTTTATTA
    AGGAAAGAAA GTATTATATG CAAACCAAAG AGTGGTTCTG CCAGTAGGCA AGTGAATTCA
    AATCCTCGGC CTCGGTCTAA CCCGGAACTC CATGGTATCT ATGTAATCAA TGTGCAGTGC
    CAGTTCTGTG AGTATGACGT ATGCCTGGAG CCAGCAAAAA CCCTGATTGA GTTTCAGAAC
    TGGGATACTC TTTTGATTTG CATTCAGGAA GGAGTAAAAA CGTTTTTAAA GAAAGAAAAA
    TTATTTGTGG AATTATCAAG TGAAGACATT AAGGAATTTA GTGAAGATAA TGATTTTAGC
    TTGTTTAGTG CCAGTCTTCA GAAGCAAGTG TCCTCTGATG AGAAGTGTGG GCAGGTCAGT
    TTCCAAGAAG CATGTAATAA TATTTTGGAT TCCTACGAGA TGTTCAATTT GCAGTCAAAA
    GCTGTGAAAA GAAAAGCTCC TGTAGAAAAC ATAAGCACAC AAAATTCTAG GGATTCAGAA
    GCTATCAGAA AAAAGACAAA TGATTCATTT TTGTACACTT ACAAATCTGA TGGGCCAGCC
    CATTGTAAAA TGGCAGAGTC ATCTTTGCAA AATGAAGACA GTGCTTGCTC ACAGTCAAGG
    AACCTAGAAC AAGAGACAGC TGGAGCATCA GAATTGGGAG AAAATAAGAA ACATAAAAAA
    TCTTGCTTGG AACATAACTC TTCAGAAAAT CCATGTGGAA CCACTTCAGA AATGTTTGGA
    AGCCCTTTTC AGACATTATA TCACTTTGAG GGGAGTGGAG AAGATCTAGA AATACAGAAA
    GTAAGTACTA CTGTTAATAG CATGGCTGCC AACATCCTGG AAAATAACAG AATTCAGAAT
    CAACCAGAGA GATTTAAAGA TGCTACTGAA ATGGATTGCC AACCTCTGTC TTTTGAGACA
    GCATTATCAG GAGAACCTAG TGCTCAGAGA GAGGAAGAGA AAAGAAAAAA ACCTAGTACT
    TGTGGAAGAA TAAATGTTTT TCGTTATGGA CAAGTTAAAT TATGTTCCAC TGGCTTCATA
    ACTCATGTGG TACAGAATGA GCAAACTAAA TTAACTGAAA CAGAACATTT ATTTAAAAGT
    TATGCTCAAC CTGGTCCTGT GAGTGCCAAA GAAACATTTA GAAATAGAAC ACACCATTCA
    GTTGAGACTC CCAACAGCAA AGATTTAACC AGCACTTTAA GTAAAGAATT TGCTGAACTA
    CCCAACAAAA GTCTGTACAG CACAAATCGA AGTTATGAGC TAGAGAACAA ACATACAGCA
    GCCTGTAAAA ATTTTGCTAT TTTTCAGGAA AGTGGTAAAA AATCACACAC AGGTGGCTTA
    TTACCTAACA CATCCTCTTT TTCTTGGGGT GGACATGTTT CAGATGGTAG TAAGAAAACA
    GACAAGTTGA TTGGTCCTGC CGAACCCATA CCTCATAAGA AGCTAAGCTT AAGCTCACAA
    CTAGGATCTT TAGAGAAGTT TAAGAGGCAA TATGGGAAGG TTAAAAATCC TCTGAATACT
    GAAGTGGAGG AAAATAATAC TTTAGAAATC ACTACCAATC TCAGTCCTCA GGTTGAAGCT
    GACATTCCAT GGAAAGACAA AAACCACTTA GACAACTCTG ACATCTGTGA AATCACTACT
    ATGAAATATA ATGATTCCTA TGGTAGTTGT CAACCAGTAA ATCACATGTT TTCTTCAGAA
    AAGCTTCCAT TCTCCAAGGA AGATGATTGT TTGGAACAAC AGATGCTTTG CTTAACAGAA
    AGCACTGTAA CTCTACAGGA GTTACCTCAT TTTAACAGTA AATTTTTGGA TGTTGAGAAG
    TCACCTGAAT CACTAGCCTC TAAATTATCC AGATTGAAGG GTTCCGAAAG AGAGACTCAA
    AGGATGGATG TGAATCATTT TAATGAATTG CTACAATTAG ATTCCAGTAG GAAAGATGGT
    GACTTGTCCA GTGGGTTAAC CCTAGATTCT TGTAAGTTAT TTAAAAATGA GCATAAAAAC
    CCAGAAAGTG ACAGTATCCC AATGTCAGAC TCTGTCACAC AAGGTAATCT CTTCAGTAAA
    GACAGTGAAA CATATTCTAA CAACAATACC ACTGAGAAGA TACCAGAAAA TCCTTTGGTA
    CTGCCCTATG ACCATGCTAC AGTTATCAGT AAGGATTCAG ATATTCTTAT AGCTTCAAAA
    CAACAGATTG GAAGTCCTGA CTCTCTGAGT AGAATGTTAA TGAGTCGCGT GGAAGATTCC
    ACAGCTGACC AAAATGGAAC TTGTTTTCAG AGTGAGGAAT CCATAGCAAG AACTTGTTCT
    GAAAATGAAG AGTCCAACAT ATGTTCTTTG GATTGGCAGC AGCATTTTGA TGTAACTCTG
    GGAAGAATGG TTTACATCAA CAAACTAACA GGACTTAGCA CATTCACTGC TCCTACTGAG
    GATGTTCAGG CTGCTTGTAC TAAAGACCTG ACAACTATGG CTGTGGATGT TCTACTGGAG
    AATGGATCTC AGTACAGGTG TCATCCCTTT AGAAGCGACC TTGTTCTTCC TTTCCTTCCT
    AGAGCTCGGG AAGAGAGGAC TGTGATGAGA CAGAATAACA GAGCTACTGG GGACGATGCC
    TTTGGTCCTG AATCACTTCA ATCTTTGTTC TCAGAATGGG ACAATCCAGT ATTTGCCCGT
    TATCCAGAGG TTGCTGTTGA CGTAAGCAGC GGCCAAGCTG AGAGCCTAGC AGTTAAAATT
    CACAACATCT TGTATCCTTA TCGTTTCACC AAAGAAATGA TTCACTCAAT GCAGGTTCTC
    CAGCAAGTGG ATAACAAGTT TATTGCCTGT TTAATGAGCA CTAAGACTGA AGAGAATGGT
    GAGGCAGATT CCTATGAAAA GCAACAGCCA CAAGGCTTTG GTCGAAAAAA ATTACTGTCT
    TCCATTGTAA GTCCTCCACT AGAGATAACA GTGACAGAAG AACAAAGGAG ACTCTTACGG
    TGTTACCACA AAAATCTGGA AGATCTGGGC CTTGAAATTG TATTTCCAGA CACCAGTGAT
    TCACTGGTCC TCATAGGAAA AGTACCACTC TGTTTTGTAG AAAGAGAAGC AAGTGAACTT
    CGAAGAGGAA GATCTACTGT GACCAAGGGC ATTGTGGAGG AGTTTATTCG AGAACAAGTG
    GAGCTACTCC AGACCACAGG AGGCATCCAA GGGACTTTGC CACTGACTGT CCAGAAGGTG
    TTGGCATCCC AAGCCTGCCA TGGGGCCATT AAGTTTAACG ATGGCCTGAG CTTCGAGGAG
    AGCTGCCGCC TTATTGAAGC GCTGTCCTGG TGCCAGCTGC CGTTCCAGTG TGCTCATGGC
    AGGCCCTCTA TGCTGCCATT AGCTAACATC GACCACTTGG AGCAGGAAAA ACAGACTAAA
    CCCAATCTTG CTAAACTTCG CAGAATGGCT CAAGCCTGGC ATCTCTTTGG AAAGGAGAAG
    GATGTGATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807909.1
    CDS11..4402
    Translation

    Target ORF information:

    RefSeq Version XM_010809607.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809607.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGTAA GTCCCAAAAG TTAAGAGAAA TAAATTTTAA ATATAAGGAG
    TTTGAGCTTA CTGGCTTTAT CAGCTCCGAA GCACATTATA ATAAGAATAT GCAGTTTTTG
    TTTGTGAACA AGAGGTTAGT TTTAAGGACA AAGTTGCATA AACTCATTGA CTTTTTATTA
    AGGAAAGAAA GTATTATATG CAAACCAAAG AGTGGTTCTG CCAGTAGGCA AGTGAATTCA
    AATCCTCGGC CTCGGTCTAA CCCGGAACTC CATGGTATCT ATGTAATCAA TGTGCAGTGC
    CAGTTCTGTG AGTATGACGT ATGCCTGGAG CCAGCAAAAA CCCTGATTGA GTTTCAGAAC
    TGGGATACTC TTTTGATTTG CATTCAGGAA GGAGTAAAAA CGTTTTTAAA GAAAGAAAAA
    TTATTTGTGG AATTATCAAG TGAAGACATT AAGGAATTTA GTGAAGATAA TGATTTTAGC
    TTGTTTAGTG CCAGTCTTCA GAAGCAAGTG TCCTCTGATG AGAAGTGTGG GCAGGTCAGT
    TTCCAAGAAG CATGTAATAA TATTTTGGAT TCCTACGAGA TGTTCAATTT GCAGTCAAAA
    GCTGTGAAAA GAAAAGCTCC TGTAGAAAAC ATAAGCACAC AAAATTCTAG GGATTCAGAA
    GCTATCAGAA AAAAGACAAA TGATTCATTT TTGTACACTT ACAAATCTGA TGGGCCAGCC
    CATTGTAAAA TGGCAGAGTC ATCTTTGCAA AATGAAGACA GTGCTTGCTC ACAGTCAAGG
    AACCTAGAAC AAGAGACAGC TGGAGCATCA GAATTGGGAG AAAATAAGAA ACATAAAAAA
    TCTTGCTTGG AACATAACTC TTCAGAAAAT CCATGTGGAA CCACTTCAGA AATGTTTGGA
    AGCCCTTTTC AGACATTATA TCACTTTGAG GGGAGTGGAG AAGATCTAGA AATACAGAAA
    GTAAGTACTA CTGTTAATAG CATGGCTGCC AACATCCTGG AAAATAACAG AATTCAGAAT
    CAACCAGAGA GATTTAAAGA TGCTACTGAA ATGGATTGCC AACCTCTGTC TTTTGAGACA
    GCATTATCAG GAGAACCTAG TGCTCAGAGA GAGGAAGAGA AAAGAAAAAA ACCTAGTACT
    TGTGGAAGAA TAAATGTTTT TCGTTATGGA CAAGTTAAAT TATGTTCCAC TGGCTTCATA
    ACTCATGTGG TACAGAATGA GCAAACTAAA TTAACTGAAA CAGAACATTT ATTTAAAAGT
    TATGCTCAAC CTGGTCCTGT GAGTGCCAAA GAAACATTTA GAAATAGAAC ACACCATTCA
    GTTGAGACTC CCAACAGCAA AGATTTAACC AGCACTTTAA GTAAAGAATT TGCTGAACTA
    CCCAACAAAA GTCTGTACAG CACAAATCGA AGTTATGAGC TAGAGAACAA ACATACAGCA
    GCCTGTAAAA ATTTTGCTAT TTTTCAGGAA AGTGGTAAAA AATCACACAC AGGTGGCTTA
    TTACCTAACA CATCCTCTTT TTCTTGGGGT GGACATGTTT CAGATGGTAG TAAGAAAACA
    GACAAGTTGA TTGGTCCTGC CGAACCCATA CCTCATAAGA AGCTAAGCTT AAGCTCACAA
    CTAGGATCTT TAGAGAAGTT TAAGAGGCAA TATGGGAAGG TTAAAAATCC TCTGAATACT
    GAAGTGGAGG AAAATAATAC TTTAGAAATC ACTACCAATC TCAGTCCTCA GGTTGAAGCT
    GACATTCCAT GGAAAGACAA AAACCACTTA GACAACTCTG ACATCTGTGA AATCACTACT
    ATGAAATATA ATGATTCCTA TGGTAGTTGT CAACCAGTAA ATCACATGTT TTCTTCAGAA
    AAGCTTCCAT TCTCCAAGGA AGATGATTGT TTGGAACAAC AGATGCTTTG CTTAACAGAA
    AGCACTGTAA CTCTACAGGA GTTACCTCAT TTTAACAGTA AATTTTTGGA TGTTGAGAAG
    TCACCTGAAT CACTAGCCTC TAAATTATCC AGATTGAAGG GTTCCGAAAG AGAGACTCAA
    AGGATGGATG TGAATCATTT TAATGAATTG CTACAATTAG ATTCCAGTAG GAAAGATGGT
    GACTTGTCCA GTGGGTTAAC CCTAGATTCT TGTAAGTTAT TTAAAAATGA GCATAAAAAC
    CCAGAAAGTG ACAGTATCCC AATGTCAGAC TCTGTCACAC AAGGTAATCT CTTCAGTAAA
    GACAGTGAAA CATATTCTAA CAACAATACC ACTGAGAAGA TACCAGAAAA TCCTTTGGTA
    CTGCCCTATG ACCATGCTAC AGTTATCAGT AAGGATTCAG ATATTCTTAT AGCTTCAAAA
    CAACAGATTG GAAGTCCTGA CTCTCTGAGT AGAATGTTAA TGAGTCGCGT GGAAGATTCC
    ACAGCTGACC AAAATGGAAC TTGTTTTCAG AGTGAGGAAT CCATAGCAAG AACTTGTTCT
    GAAAATGAAG AGTCCAACAT ATGTTCTTTG GATTGGCAGC AGCATTTTGA TGTAACTCTG
    GGAAGAATGG TTTACATCAA CAAACTAACA GGACTTAGCA CATTCACTGC TCCTACTGAG
    GATGTTCAGG CTGCTTGTAC TAAAGACCTG ACAACTATGG CTGTGGATGT TCTACTGGAG
    AATGGATCTC AGTACAGGTG TCATCCCTTT AGAAGCGACC TTGTTCTTCC TTTCCTTCCT
    AGAGCTCGGG AAGAGAGGAC TGTGATGAGA CAGAATAACA GAGCTACTGG GGACGATGCC
    TTTGGTCCTG AATCACTTCA ATCTTTGTTC TCAGAATGGG ACAATCCAGT ATTTGCCCGT
    TATCCAGAGG TTGCTGTTGA CGTAAGCAGC GGCCAAGCTG AGAGCCTAGC AGTTAAAATT
    CACAACATCT TGTATCCTTA TCGTTTCACC AAAGAAATGA TTCACTCAAT GCAGGTTCTC
    CAGCAAGTGG ATAACAAGTT TATTGCCTGT TTAATGAGCA CTAAGACTGA AGAGAATGGT
    GAGGCAGATT CCTATGAAAA GCAACAGCCA CAAGGCTTTG GTCGAAAAAA ATTACTGTCT
    TCCATTGTAA GTCCTCCACT AGAGATAACA GTGACAGAAG AACAAAGGAG ACTCTTACGG
    TGTTACCACA AAAATCTGGA AGATCTGGGC CTTGAAATTG TATTTCCAGA CACCAGTGAT
    TCACTGGTCC TCATAGGAAA AGTACCACTC TGTTTTGTAG AAAGAGAAGC AAGTGAACTT
    CGAAGAGGAA GATCTACTGT GACCAAGGGC ATTGTGGAGG AGTTTATTCG AGAACAAGTG
    GAGCTACTCC AGACCACAGG AGGCATCCAA GGGACTTTGC CACTGACTGT CCAGAAGGTG
    TTGGCATCCC AAGCCTGCCA TGGGGCCATT AAGTTTAACG ATGGCCTGAG CTTCGAGGAG
    AGCTGCCGCC TTATTGAAGC GCTGTCCTGG TGCCAGCTGC CGTTCCAGTG TGCTCATGGC
    AGGCCCTCTA TGCTGCCATT AGCTAACATC GACCACTTGG AGCAGGAAAA ACAGACTAAA
    CCCAATCTTG CTAAACTTCG CAGAATGGCT CAAGCCTGGC ATCTCTTTGG AAAGGAGAAG
    GATGTGATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57926
    Clone ID Related Accession (Same CDS sequence) XM_010809607.2 , XM_010813177.2 , XM_010809607.3
    Accession Version XM_010813177.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGTAA GTCCCAAAAG TTAAGAGAAA TAAATTTTAA ATATAAGGAG
    TTTGAGCTTA CTGGCTTTAT CAGCTCCGAA GCACATTATA ATAAGAATAT GCAGTTTTTG
    TTTGTGAACA AGAGGTTAGT TTTAAGGACA AAGTTGCATA AACTCATTGA CTTTTTATTA
    AGGAAAGAAA GTATTATATG CAAACCAAAG AGTGGTTCTG CCAGTAGGCA AGTGAATTCA
    AATCCTCGGC CTCGGTCTAA CCCGGAACTC CATGGTATCT ATGTAATCAA TGTGCAGTGC
    CAGTTCTGTG AGTATGACGT ATGCCTGGAG CCAGCAAAAA CCCTGATTGA GTTTCAGAAC
    TGGGATACTC TTTTGATTTG CATTCAGGAA GGAGTAAAAA CGTTTTTAAA GAAAGAAAAA
    TTATTTGTGG AATTATCAAG TGAAGACATT AAGGAATTTA GTGAAGATAA TGATTTTAGC
    TTGTTTAGTG CCAGTCTTCA GAAGCAAGTG TCCTCTGATG AGAAGTGTGG GCAGGTCAGT
    TTCCAAGAAG CATGTAATAA TATTTTGGAT TCCTACGAGA TGTTCAATTT GCAGTCAAAA
    GCTGTGAAAA GAAAAGCTCC TGTAGAAAAC ATAAGCACAC AAAATTCTAG GGATTCAGAA
    GCTATCAGAA AAAAGACAAA TGATTCATTT TTGTACACTT ACAAATCTGA TGGGCCAGCC
    CATTGTAAAA TGGCAGAGTC ATCTTTGCAA AATGAAGACA GTGCTTGCTC ACAGTCAAGG
    AACCTAGAAC AAGAGACAGC TGGAGCATCA GAATTGGGAG AAAATAAGAA ACATAAAAAA
    TCTTGCTTGG AACATAACTC TTCAGAAAAT CCATGTGGAA CCACTTCAGA AATGTTTGGA
    AGCCCTTTTC AGACATTATA TCACTTTGAG GGGAGTGGAG AAGATCTAGA AATACAGAAA
    GTAAGTACTA CTGTTAATAG CATGGCTGCC AACATCCTGG AAAATAACAG AATTCAGAAT
    CAACCAGAGA GATTTAAAGA TGCTACTGAA ATGGATTGCC AACCTCTGTC TTTTGAGACA
    GCATTATCAG GAGAACCTAG TGCTCAGAGA GAGGAAGAGA AAAGAAAAAA ACCTAGTACT
    TGTGGAAGAA TAAATGTTTT TCGTTATGGA CAAGTTAAAT TATGTTCCAC TGGCTTCATA
    ACTCATGTGG TACAGAATGA GCAAACTAAA TTAACTGAAA CAGAACATTT ATTTAAAAGT
    TATGCTCAAC CTGGTCCTGT GAGTGCCAAA GAAACATTTA GAAATAGAAC ACACCATTCA
    GTTGAGACTC CCAACAGCAA AGATTTAACC AGCACTTTAA GTAAAGAATT TGCTGAACTA
    CCCAACAAAA GTCTGTACAG CACAAATCGA AGTTATGAGC TAGAGAACAA ACATACAGCA
    GCCTGTAAAA ATTTTGCTAT TTTTCAGGAA AGTGGTAAAA AATCACACAC AGGTGGCTTA
    TTACCTAACA CATCCTCTTT TTCTTGGGGT GGACATGTTT CAGATGGTAG TAAGAAAACA
    GACAAGTTGA TTGGTCCTGC CGAACCCATA CCTCATAAGA AGCTAAGCTT AAGCTCACAA
    CTAGGATCTT TAGAGAAGTT TAAGAGGCAA TATGGGAAGG TTAAAAATCC TCTGAATACT
    GAAGTGGAGG AAAATAATAC TTTAGAAATC ACTACCAATC TCAGTCCTCA GGTTGAAGCT
    GACATTCCAT GGAAAGACAA AAACCACTTA GACAACTCTG ACATCTGTGA AATCACTACT
    ATGAAATATA ATGATTCCTA TGGTAGTTGT CAACCAGTAA ATCACATGTT TTCTTCAGAA
    AAGCTTCCAT TCTCCAAGGA AGATGATTGT TTGGAACAAC AGATGCTTTG CTTAACAGAA
    AGCACTGTAA CTCTACAGGA GTTACCTCAT TTTAACAGTA AATTTTTGGA TGTTGAGAAG
    TCACCTGAAT CACTAGCCTC TAAATTATCC AGATTGAAGG GTTCCGAAAG AGAGACTCAA
    AGGATGGATG TGAATCATTT TAATGAATTG CTACAATTAG ATTCCAGTAG GAAAGATGGT
    GACTTGTCCA GTGGGTTAAC CCTAGATTCT TGTAAGTTAT TTAAAAATGA GCATAAAAAC
    CCAGAAAGTG ACAGTATCCC AATGTCAGAC TCTGTCACAC AAGGTAATCT CTTCAGTAAA
    GACAGTGAAA CATATTCTAA CAACAATACC ACTGAGAAGA TACCAGAAAA TCCTTTGGTA
    CTGCCCTATG ACCATGCTAC AGTTATCAGT AAGGATTCAG ATATTCTTAT AGCTTCAAAA
    CAACAGATTG GAAGTCCTGA CTCTCTGAGT AGAATGTTAA TGAGTCGCGT GGAAGATTCC
    ACAGCTGACC AAAATGGAAC TTGTTTTCAG AGTGAGGAAT CCATAGCAAG AACTTGTTCT
    GAAAATGAAG AGTCCAACAT ATGTTCTTTG GATTGGCAGC AGCATTTTGA TGTAACTCTG
    GGAAGAATGG TTTACATCAA CAAACTAACA GGACTTAGCA CATTCACTGC TCCTACTGAG
    GATGTTCAGG CTGCTTGTAC TAAAGACCTG ACAACTATGG CTGTGGATGT TCTACTGGAG
    AATGGATCTC AGTACAGGTG TCATCCCTTT AGAAGCGACC TTGTTCTTCC TTTCCTTCCT
    AGAGCTCGGG AAGAGAGGAC TGTGATGAGA CAGAATAACA GAGCTACTGG GGACGATGCC
    TTTGGTCCTG AATCACTTCA ATCTTTGTTC TCAGAATGGG ACAATCCAGT ATTTGCCCGT
    TATCCAGAGG TTGCTGTTGA CGTAAGCAGC GGCCAAGCTG AGAGCCTAGC AGTTAAAATT
    CACAACATCT TGTATCCTTA TCGTTTCACC AAAGAAATGA TTCACTCAAT GCAGGTTCTC
    CAGCAAGTGG ATAACAAGTT TATTGCCTGT TTAATGAGCA CTAAGACTGA AGAGAATGGT
    GAGGCAGATT CCTATGAAAA GCAACAGCCA CAAGGCTTTG GTCGAAAAAA ATTACTGTCT
    TCCATTGTAA GTCCTCCACT AGAGATAACA GTGACAGAAG AACAAAGGAG ACTCTTACGG
    TGTTACCACA AAAATCTGGA AGATCTGGGC CTTGAAATTG TATTTCCAGA CACCAGTGAT
    TCACTGGTCC TCATAGGAAA AGTACCACTC TGTTTTGTAG AAAGAGAAGC AAGTGAACTT
    CGAAGAGGAA GATCTACTGT GACCAAGGGC ATTGTGGAGG AGTTTATTCG AGAACAAGTG
    GAGCTACTCC AGACCACAGG AGGCATCCAA GGGACTTTGC CACTGACTGT CCAGAAGGTG
    TTGGCATCCC AAGCCTGCCA TGGGGCCATT AAGTTTAACG ATGGCCTGAG CTTCGAGGAG
    AGCTGCCGCC TTATTGAAGC GCTGTCCTGG TGCCAGCTGC CGTTCCAGTG TGCTCATGGC
    AGGCCCTCTA TGCTGCCATT AGCTAACATC GACCACTTGG AGCAGGAAAA ACAGACTAAA
    CCCAATCTTG CTAAACTTCG CAGAATGGCT CAAGCCTGGC ATCTCTTTGG AAAGGAGAAG
    GATGTGATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811479.1
    CDS11..4402
    Translation

    Target ORF information:

    RefSeq Version XM_010813177.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813177.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGTAA GTCCCAAAAG TTAAGAGAAA TAAATTTTAA ATATAAGGAG
    TTTGAGCTTA CTGGCTTTAT CAGCTCCGAA GCACATTATA ATAAGAATAT GCAGTTTTTG
    TTTGTGAACA AGAGGTTAGT TTTAAGGACA AAGTTGCATA AACTCATTGA CTTTTTATTA
    AGGAAAGAAA GTATTATATG CAAACCAAAG AGTGGTTCTG CCAGTAGGCA AGTGAATTCA
    AATCCTCGGC CTCGGTCTAA CCCGGAACTC CATGGTATCT ATGTAATCAA TGTGCAGTGC
    CAGTTCTGTG AGTATGACGT ATGCCTGGAG CCAGCAAAAA CCCTGATTGA GTTTCAGAAC
    TGGGATACTC TTTTGATTTG CATTCAGGAA GGAGTAAAAA CGTTTTTAAA GAAAGAAAAA
    TTATTTGTGG AATTATCAAG TGAAGACATT AAGGAATTTA GTGAAGATAA TGATTTTAGC
    TTGTTTAGTG CCAGTCTTCA GAAGCAAGTG TCCTCTGATG AGAAGTGTGG GCAGGTCAGT
    TTCCAAGAAG CATGTAATAA TATTTTGGAT TCCTACGAGA TGTTCAATTT GCAGTCAAAA
    GCTGTGAAAA GAAAAGCTCC TGTAGAAAAC ATAAGCACAC AAAATTCTAG GGATTCAGAA
    GCTATCAGAA AAAAGACAAA TGATTCATTT TTGTACACTT ACAAATCTGA TGGGCCAGCC
    CATTGTAAAA TGGCAGAGTC ATCTTTGCAA AATGAAGACA GTGCTTGCTC ACAGTCAAGG
    AACCTAGAAC AAGAGACAGC TGGAGCATCA GAATTGGGAG AAAATAAGAA ACATAAAAAA
    TCTTGCTTGG AACATAACTC TTCAGAAAAT CCATGTGGAA CCACTTCAGA AATGTTTGGA
    AGCCCTTTTC AGACATTATA TCACTTTGAG GGGAGTGGAG AAGATCTAGA AATACAGAAA
    GTAAGTACTA CTGTTAATAG CATGGCTGCC AACATCCTGG AAAATAACAG AATTCAGAAT
    CAACCAGAGA GATTTAAAGA TGCTACTGAA ATGGATTGCC AACCTCTGTC TTTTGAGACA
    GCATTATCAG GAGAACCTAG TGCTCAGAGA GAGGAAGAGA AAAGAAAAAA ACCTAGTACT
    TGTGGAAGAA TAAATGTTTT TCGTTATGGA CAAGTTAAAT TATGTTCCAC TGGCTTCATA
    ACTCATGTGG TACAGAATGA GCAAACTAAA TTAACTGAAA CAGAACATTT ATTTAAAAGT
    TATGCTCAAC CTGGTCCTGT GAGTGCCAAA GAAACATTTA GAAATAGAAC ACACCATTCA
    GTTGAGACTC CCAACAGCAA AGATTTAACC AGCACTTTAA GTAAAGAATT TGCTGAACTA
    CCCAACAAAA GTCTGTACAG CACAAATCGA AGTTATGAGC TAGAGAACAA ACATACAGCA
    GCCTGTAAAA ATTTTGCTAT TTTTCAGGAA AGTGGTAAAA AATCACACAC AGGTGGCTTA
    TTACCTAACA CATCCTCTTT TTCTTGGGGT GGACATGTTT CAGATGGTAG TAAGAAAACA
    GACAAGTTGA TTGGTCCTGC CGAACCCATA CCTCATAAGA AGCTAAGCTT AAGCTCACAA
    CTAGGATCTT TAGAGAAGTT TAAGAGGCAA TATGGGAAGG TTAAAAATCC TCTGAATACT
    GAAGTGGAGG AAAATAATAC TTTAGAAATC ACTACCAATC TCAGTCCTCA GGTTGAAGCT
    GACATTCCAT GGAAAGACAA AAACCACTTA GACAACTCTG ACATCTGTGA AATCACTACT
    ATGAAATATA ATGATTCCTA TGGTAGTTGT CAACCAGTAA ATCACATGTT TTCTTCAGAA
    AAGCTTCCAT TCTCCAAGGA AGATGATTGT TTGGAACAAC AGATGCTTTG CTTAACAGAA
    AGCACTGTAA CTCTACAGGA GTTACCTCAT TTTAACAGTA AATTTTTGGA TGTTGAGAAG
    TCACCTGAAT CACTAGCCTC TAAATTATCC AGATTGAAGG GTTCCGAAAG AGAGACTCAA
    AGGATGGATG TGAATCATTT TAATGAATTG CTACAATTAG ATTCCAGTAG GAAAGATGGT
    GACTTGTCCA GTGGGTTAAC CCTAGATTCT TGTAAGTTAT TTAAAAATGA GCATAAAAAC
    CCAGAAAGTG ACAGTATCCC AATGTCAGAC TCTGTCACAC AAGGTAATCT CTTCAGTAAA
    GACAGTGAAA CATATTCTAA CAACAATACC ACTGAGAAGA TACCAGAAAA TCCTTTGGTA
    CTGCCCTATG ACCATGCTAC AGTTATCAGT AAGGATTCAG ATATTCTTAT AGCTTCAAAA
    CAACAGATTG GAAGTCCTGA CTCTCTGAGT AGAATGTTAA TGAGTCGCGT GGAAGATTCC
    ACAGCTGACC AAAATGGAAC TTGTTTTCAG AGTGAGGAAT CCATAGCAAG AACTTGTTCT
    GAAAATGAAG AGTCCAACAT ATGTTCTTTG GATTGGCAGC AGCATTTTGA TGTAACTCTG
    GGAAGAATGG TTTACATCAA CAAACTAACA GGACTTAGCA CATTCACTGC TCCTACTGAG
    GATGTTCAGG CTGCTTGTAC TAAAGACCTG ACAACTATGG CTGTGGATGT TCTACTGGAG
    AATGGATCTC AGTACAGGTG TCATCCCTTT AGAAGCGACC TTGTTCTTCC TTTCCTTCCT
    AGAGCTCGGG AAGAGAGGAC TGTGATGAGA CAGAATAACA GAGCTACTGG GGACGATGCC
    TTTGGTCCTG AATCACTTCA ATCTTTGTTC TCAGAATGGG ACAATCCAGT ATTTGCCCGT
    TATCCAGAGG TTGCTGTTGA CGTAAGCAGC GGCCAAGCTG AGAGCCTAGC AGTTAAAATT
    CACAACATCT TGTATCCTTA TCGTTTCACC AAAGAAATGA TTCACTCAAT GCAGGTTCTC
    CAGCAAGTGG ATAACAAGTT TATTGCCTGT TTAATGAGCA CTAAGACTGA AGAGAATGGT
    GAGGCAGATT CCTATGAAAA GCAACAGCCA CAAGGCTTTG GTCGAAAAAA ATTACTGTCT
    TCCATTGTAA GTCCTCCACT AGAGATAACA GTGACAGAAG AACAAAGGAG ACTCTTACGG
    TGTTACCACA AAAATCTGGA AGATCTGGGC CTTGAAATTG TATTTCCAGA CACCAGTGAT
    TCACTGGTCC TCATAGGAAA AGTACCACTC TGTTTTGTAG AAAGAGAAGC AAGTGAACTT
    CGAAGAGGAA GATCTACTGT GACCAAGGGC ATTGTGGAGG AGTTTATTCG AGAACAAGTG
    GAGCTACTCC AGACCACAGG AGGCATCCAA GGGACTTTGC CACTGACTGT CCAGAAGGTG
    TTGGCATCCC AAGCCTGCCA TGGGGCCATT AAGTTTAACG ATGGCCTGAG CTTCGAGGAG
    AGCTGCCGCC TTATTGAAGC GCTGTCCTGG TGCCAGCTGC CGTTCCAGTG TGCTCATGGC
    AGGCCCTCTA TGCTGCCATT AGCTAACATC GACCACTTGG AGCAGGAAAA ACAGACTAAA
    CCCAATCTTG CTAAACTTCG CAGAATGGCT CAAGCCTGGC ATCTCTTTGG AAAGGAGAAG
    GATGTGATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57926
    Clone ID Related Accession (Same CDS sequence) XM_010809607.2 , XM_010813177.2 , XM_010809607.3
    Accession Version XM_010809607.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA mismatch repair protein Mlh3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809607.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGTAA GTCCCAAAAG TTAAGAGAAA TAAATTTTAA ATATAAGGAG
    TTTGAGCTTA CTGGCTTTAT CAGCTCCGAA GCACATTATA ATAAGAATAT GCAGTTTTTG
    TTTGTGAACA AGAGGTTAGT TTTAAGGACA AAGTTGCATA AACTCATTGA CTTTTTATTA
    AGGAAAGAAA GTATTATATG CAAACCAAAG AGTGGTTCTG CCAGTAGGCA AGTGAATTCA
    AATCCTCGGC CTCGGTCTAA CCCGGAACTC CATGGTATCT ATGTAATCAA TGTGCAGTGC
    CAGTTCTGTG AGTATGACGT ATGCCTGGAG CCAGCAAAAA CCCTGATTGA GTTTCAGAAC
    TGGGATACTC TTTTGATTTG CATTCAGGAA GGAGTAAAAA CGTTTTTAAA GAAAGAAAAA
    TTATTTGTGG AATTATCAAG TGAAGACATT AAGGAATTTA GTGAAGATAA TGATTTTAGC
    TTGTTTAGTG CCAGTCTTCA GAAGCAAGTG TCCTCTGATG AGAAGTGTGG GCAGGTCAGT
    TTCCAAGAAG CATGTAATAA TATTTTGGAT TCCTACGAGA TGTTCAATTT GCAGTCAAAA
    GCTGTGAAAA GAAAAGCTCC TGTAGAAAAC ATAAGCACAC AAAATTCTAG GGATTCAGAA
    GCTATCAGAA AAAAGACAAA TGATTCATTT TTGTACACTT ACAAATCTGA TGGGCCAGCC
    CATTGTAAAA TGGCAGAGTC ATCTTTGCAA AATGAAGACA GTGCTTGCTC ACAGTCAAGG
    AACCTAGAAC AAGAGACAGC TGGAGCATCA GAATTGGGAG AAAATAAGAA ACATAAAAAA
    TCTTGCTTGG AACATAACTC TTCAGAAAAT CCATGTGGAA CCACTTCAGA AATGTTTGGA
    AGCCCTTTTC AGACATTATA TCACTTTGAG GGGAGTGGAG AAGATCTAGA AATACAGAAA
    GTAAGTACTA CTGTTAATAG CATGGCTGCC AACATCCTGG AAAATAACAG AATTCAGAAT
    CAACCAGAGA GATTTAAAGA TGCTACTGAA ATGGATTGCC AACCTCTGTC TTTTGAGACA
    GCATTATCAG GAGAACCTAG TGCTCAGAGA GAGGAAGAGA AAAGAAAAAA ACCTAGTACT
    TGTGGAAGAA TAAATGTTTT TCGTTATGGA CAAGTTAAAT TATGTTCCAC TGGCTTCATA
    ACTCATGTGG TACAGAATGA GCAAACTAAA TTAACTGAAA CAGAACATTT ATTTAAAAGT
    TATGCTCAAC CTGGTCCTGT GAGTGCCAAA GAAACATTTA GAAATAGAAC ACACCATTCA
    GTTGAGACTC CCAACAGCAA AGATTTAACC AGCACTTTAA GTAAAGAATT TGCTGAACTA
    CCCAACAAAA GTCTGTACAG CACAAATCGA AGTTATGAGC TAGAGAACAA ACATACAGCA
    GCCTGTAAAA ATTTTGCTAT TTTTCAGGAA AGTGGTAAAA AATCACACAC AGGTGGCTTA
    TTACCTAACA CATCCTCTTT TTCTTGGGGT GGACATGTTT CAGATGGTAG TAAGAAAACA
    GACAAGTTGA TTGGTCCTGC CGAACCCATA CCTCATAAGA AGCTAAGCTT AAGCTCACAA
    CTAGGATCTT TAGAGAAGTT TAAGAGGCAA TATGGGAAGG TTAAAAATCC TCTGAATACT
    GAAGTGGAGG AAAATAATAC TTTAGAAATC ACTACCAATC TCAGTCCTCA GGTTGAAGCT
    GACATTCCAT GGAAAGACAA AAACCACTTA GACAACTCTG ACATCTGTGA AATCACTACT
    ATGAAATATA ATGATTCCTA TGGTAGTTGT CAACCAGTAA ATCACATGTT TTCTTCAGAA
    AAGCTTCCAT TCTCCAAGGA AGATGATTGT TTGGAACAAC AGATGCTTTG CTTAACAGAA
    AGCACTGTAA CTCTACAGGA GTTACCTCAT TTTAACAGTA AATTTTTGGA TGTTGAGAAG
    TCACCTGAAT CACTAGCCTC TAAATTATCC AGATTGAAGG GTTCCGAAAG AGAGACTCAA
    AGGATGGATG TGAATCATTT TAATGAATTG CTACAATTAG ATTCCAGTAG GAAAGATGGT
    GACTTGTCCA GTGGGTTAAC CCTAGATTCT TGTAAGTTAT TTAAAAATGA GCATAAAAAC
    CCAGAAAGTG ACAGTATCCC AATGTCAGAC TCTGTCACAC AAGGTAATCT CTTCAGTAAA
    GACAGTGAAA CATATTCTAA CAACAATACC ACTGAGAAGA TACCAGAAAA TCCTTTGGTA
    CTGCCCTATG ACCATGCTAC AGTTATCAGT AAGGATTCAG ATATTCTTAT AGCTTCAAAA
    CAACAGATTG GAAGTCCTGA CTCTCTGAGT AGAATGTTAA TGAGTCGCGT GGAAGATTCC
    ACAGCTGACC AAAATGGAAC TTGTTTTCAG AGTGAGGAAT CCATAGCAAG AACTTGTTCT
    GAAAATGAAG AGTCCAACAT ATGTTCTTTG GATTGGCAGC AGCATTTTGA TGTAACTCTG
    GGAAGAATGG TTTACATCAA CAAACTAACA GGACTTAGCA CATTCACTGC TCCTACTGAG
    GATGTTCAGG CTGCTTGTAC TAAAGACCTG ACAACTATGG CTGTGGATGT TCTACTGGAG
    AATGGATCTC AGTACAGGTG TCATCCCTTT AGAAGCGACC TTGTTCTTCC TTTCCTTCCT
    AGAGCTCGGG AAGAGAGGAC TGTGATGAGA CAGAATAACA GAGCTACTGG GGACGATGCC
    TTTGGTCCTG AATCACTTCA ATCTTTGTTC TCAGAATGGG ACAATCCAGT ATTTGCCCGT
    TATCCAGAGG TTGCTGTTGA CGTAAGCAGC GGCCAAGCTG AGAGCCTAGC AGTTAAAATT
    CACAACATCT TGTATCCTTA TCGTTTCACC AAAGAAATGA TTCACTCAAT GCAGGTTCTC
    CAGCAAGTGG ATAACAAGTT TATTGCCTGT TTAATGAGCA CTAAGACTGA AGAGAATGGT
    GAGGCAGATT CCTATGAAAA GCAACAGCCA CAAGGCTTTG GTCGAAAAAA ATTACTGTCT
    TCCATTGTAA GTCCTCCACT AGAGATAACA GTGACAGAAG AACAAAGGAG ACTCTTACGG
    TGTTACCACA AAAATCTGGA AGATCTGGGC CTTGAAATTG TATTTCCAGA CACCAGTGAT
    TCACTGGTCC TCATAGGAAA AGTACCACTC TGTTTTGTAG AAAGAGAAGC AAGTGAACTT
    CGAAGAGGAA GATCTACTGT GACCAAGGGC ATTGTGGAGG AGTTTATTCG AGAACAAGTG
    GAGCTACTCC AGACCACAGG AGGCATCCAA GGGACTTTGC CACTGACTGT CCAGAAGGTG
    TTGGCATCCC AAGCCTGCCA TGGGGCCATT AAGTTTAACG ATGGCCTGAG CTTCGAGGAG
    AGCTGCCGCC TTATTGAAGC GCTGTCCTGG TGCCAGCTGC CGTTCCAGTG TGCTCATGGC
    AGGCCCTCTA TGCTGCCATT AGCTAACATC GACCACTTGG AGCAGGAAAA ACAGACTAAA
    CCCAATCTTG CTAAACTTCG CAGAATGGCT CAAGCCTGGC ATCTCTTTGG AAAGGAGAAG
    GATGTGATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807909.1
    CDS61..4452
    Translation

    Target ORF information:

    RefSeq Version XM_010809607.3
    Organism Bos taurus(cattle)
    Definition Bos taurus mutL homolog 3 (MLH3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809607.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTCGCGG GCTCGCGCGT GCACGAGCCT CACGTGTACG AGCCTCACCA GCCACCGAGC 
    GCGCGCCCGG CGTCCGAGGC GGCAATTATT TCCAGTCAGA AAAGGGAACC ACTGCCTGGC
    ATTCTTACCA GCTTTCTACC TGCCATGATC AAATGCTTGT CAGCTGAAGT ACAAGCTAGA
    TTGCGTTCTG GTTTGGCTGT ATGCTCCTTA GGCCAATGTG TTGAGGAGCT TGCCCTGAAC
    AGTATTGATG CTGAAGCAAA GTGTGTGGCT GTCAGGGTGA ACATGGAAAC CTTTCAAGTT
    CAAGTGATAG ACAATGGATT TGGGATGGGG AGTGATGATG TAGACAAGGT GGGAAATCGT
    TATTTCACCA GTAAATGCAA CTCCATACAG GACTTGGAGA ACCCAAGGTT TTATGGTTTC
    CGGGGGGAGG CGTTGGCCAG TATAGCTGAT ATGGCCAGTG CTGTGGAAAT TTCATCCAAG
    AAAAACAAGT CGATGAAAAC ATTTGTGAAA CTGTTTCAGA ATGGGAAAGC CCTGAAAGCT
    TGTGAAGCTG ACTTAACTCG ACCAAGTGCC GGGACGACAG TCACTGTATA TAATCTGTTT
    TATCAGTTAC CTGTTAGGAG GAAATGCATG GATCCTAGAC TGGAGTTTGA GAAGGTTAGG
    CAGAGGATAG AAGCTCTGTC ACTCATGCAC CCTACCATTT CTTTCTCTTT GAGAAATGAT
    GTTTCTGGTT CCATGGTTCT TCAACTCCCT AAAACCAAAG ACATATGTTC CCGATTTTGT
    CAAATTTATG GACTGGGTAA GTCCCAAAAG TTAAGAGAAA TAAATTTTAA ATATAAGGAG
    TTTGAGCTTA CTGGCTTTAT CAGCTCCGAA GCACATTATA ATAAGAATAT GCAGTTTTTG
    TTTGTGAACA AGAGGTTAGT TTTAAGGACA AAGTTGCATA AACTCATTGA CTTTTTATTA
    AGGAAAGAAA GTATTATATG CAAACCAAAG AGTGGTTCTG CCAGTAGGCA AGTGAATTCA
    AATCCTCGGC CTCGGTCTAA CCCGGAACTC CATGGTATCT ATGTAATCAA TGTGCAGTGC
    CAGTTCTGTG AGTATGACGT ATGCCTGGAG CCAGCAAAAA CCCTGATTGA GTTTCAGAAC
    TGGGATACTC TTTTGATTTG CATTCAGGAA GGAGTAAAAA CGTTTTTAAA GAAAGAAAAA
    TTATTTGTGG AATTATCAAG TGAAGACATT AAGGAATTTA GTGAAGATAA TGATTTTAGC
    TTGTTTAGTG CCAGTCTTCA GAAGCAAGTG TCCTCTGATG AGAAGTGTGG GCAGGTCAGT
    TTCCAAGAAG CATGTAATAA TATTTTGGAT TCCTACGAGA TGTTCAATTT GCAGTCAAAA
    GCTGTGAAAA GAAAAGCTCC TGTAGAAAAC ATAAGCACAC AAAATTCTAG GGATTCAGAA
    GCTATCAGAA AAAAGACAAA TGATTCATTT TTGTACACTT ACAAATCTGA TGGGCCAGCC
    CATTGTAAAA TGGCAGAGTC ATCTTTGCAA AATGAAGACA GTGCTTGCTC ACAGTCAAGG
    AACCTAGAAC AAGAGACAGC TGGAGCATCA GAATTGGGAG AAAATAAGAA ACATAAAAAA
    TCTTGCTTGG AACATAACTC TTCAGAAAAT CCATGTGGAA CCACTTCAGA AATGTTTGGA
    AGCCCTTTTC AGACATTATA TCACTTTGAG GGGAGTGGAG AAGATCTAGA AATACAGAAA
    GTAAGTACTA CTGTTAATAG CATGGCTGCC AACATCCTGG AAAATAACAG AATTCAGAAT
    CAACCAGAGA GATTTAAAGA TGCTACTGAA ATGGATTGCC AACCTCTGTC TTTTGAGACA
    GCATTATCAG GAGAACCTAG TGCTCAGAGA GAGGAAGAGA AAAGAAAAAA ACCTAGTACT
    TGTGGAAGAA TAAATGTTTT TCGTTATGGA CAAGTTAAAT TATGTTCCAC TGGCTTCATA
    ACTCATGTGG TACAGAATGA GCAAACTAAA TTAACTGAAA CAGAACATTT ATTTAAAAGT
    TATGCTCAAC CTGGTCCTGT GAGTGCCAAA GAAACATTTA GAAATAGAAC ACACCATTCA
    GTTGAGACTC CCAACAGCAA AGATTTAACC AGCACTTTAA GTAAAGAATT TGCTGAACTA
    CCCAACAAAA GTCTGTACAG CACAAATCGA AGTTATGAGC TAGAGAACAA ACATACAGCA
    GCCTGTAAAA ATTTTGCTAT TTTTCAGGAA AGTGGTAAAA AATCACACAC AGGTGGCTTA
    TTACCTAACA CATCCTCTTT TTCTTGGGGT GGACATGTTT CAGATGGTAG TAAGAAAACA
    GACAAGTTGA TTGGTCCTGC CGAACCCATA CCTCATAAGA AGCTAAGCTT AAGCTCACAA
    CTAGGATCTT TAGAGAAGTT TAAGAGGCAA TATGGGAAGG TTAAAAATCC TCTGAATACT
    GAAGTGGAGG AAAATAATAC TTTAGAAATC ACTACCAATC TCAGTCCTCA GGTTGAAGCT
    GACATTCCAT GGAAAGACAA AAACCACTTA GACAACTCTG ACATCTGTGA AATCACTACT
    ATGAAATATA ATGATTCCTA TGGTAGTTGT CAACCAGTAA ATCACATGTT TTCTTCAGAA
    AAGCTTCCAT TCTCCAAGGA AGATGATTGT TTGGAACAAC AGATGCTTTG CTTAACAGAA
    AGCACTGTAA CTCTACAGGA GTTACCTCAT TTTAACAGTA AATTTTTGGA TGTTGAGAAG
    TCACCTGAAT CACTAGCCTC TAAATTATCC AGATTGAAGG GTTCCGAAAG AGAGACTCAA
    AGGATGGATG TGAATCATTT TAATGAATTG CTACAATTAG ATTCCAGTAG GAAAGATGGT
    GACTTGTCCA GTGGGTTAAC CCTAGATTCT TGTAAGTTAT TTAAAAATGA GCATAAAAAC
    CCAGAAAGTG ACAGTATCCC AATGTCAGAC TCTGTCACAC AAGGTAATCT CTTCAGTAAA
    GACAGTGAAA CATATTCTAA CAACAATACC ACTGAGAAGA TACCAGAAAA TCCTTTGGTA
    CTGCCCTATG ACCATGCTAC AGTTATCAGT AAGGATTCAG ATATTCTTAT AGCTTCAAAA
    CAACAGATTG GAAGTCCTGA CTCTCTGAGT AGAATGTTAA TGAGTCGCGT GGAAGATTCC
    ACAGCTGACC AAAATGGAAC TTGTTTTCAG AGTGAGGAAT CCATAGCAAG AACTTGTTCT
    GAAAATGAAG AGTCCAACAT ATGTTCTTTG GATTGGCAGC AGCATTTTGA TGTAACTCTG
    GGAAGAATGG TTTACATCAA CAAACTAACA GGACTTAGCA CATTCACTGC TCCTACTGAG
    GATGTTCAGG CTGCTTGTAC TAAAGACCTG ACAACTATGG CTGTGGATGT TCTACTGGAG
    AATGGATCTC AGTACAGGTG TCATCCCTTT AGAAGCGACC TTGTTCTTCC TTTCCTTCCT
    AGAGCTCGGG AAGAGAGGAC TGTGATGAGA CAGAATAACA GAGCTACTGG GGACGATGCC
    TTTGGTCCTG AATCACTTCA ATCTTTGTTC TCAGAATGGG ACAATCCAGT ATTTGCCCGT
    TATCCAGAGG TTGCTGTTGA CGTAAGCAGC GGCCAAGCTG AGAGCCTAGC AGTTAAAATT
    CACAACATCT TGTATCCTTA TCGTTTCACC AAAGAAATGA TTCACTCAAT GCAGGTTCTC
    CAGCAAGTGG ATAACAAGTT TATTGCCTGT TTAATGAGCA CTAAGACTGA AGAGAATGGT
    GAGGCAGATT CCTATGAAAA GCAACAGCCA CAAGGCTTTG GTCGAAAAAA ATTACTGTCT
    TCCATTGTAA GTCCTCCACT AGAGATAACA GTGACAGAAG AACAAAGGAG ACTCTTACGG
    TGTTACCACA AAAATCTGGA AGATCTGGGC CTTGAAATTG TATTTCCAGA CACCAGTGAT
    TCACTGGTCC TCATAGGAAA AGTACCACTC TGTTTTGTAG AAAGAGAAGC AAGTGAACTT
    CGAAGAGGAA GATCTACTGT GACCAAGGGC ATTGTGGAGG AGTTTATTCG AGAACAAGTG
    GAGCTACTCC AGACCACAGG AGGCATCCAA GGGACTTTGC CACTGACTGT CCAGAAGGTG
    TTGGCATCCC AAGCCTGCCA TGGGGCCATT AAGTTTAACG ATGGCCTGAG CTTCGAGGAG
    AGCTGCCGCC TTATTGAAGC GCTGTCCTGG TGCCAGCTGC CGTTCCAGTG TGCTCATGGC
    AGGCCCTCTA TGCTGCCATT AGCTAACATC GACCACTTGG AGCAGGAAAA ACAGACTAAA
    CCCAATCTTG CTAAACTTCG CAGAATGGCT CAAGCCTGGC ATCTCTTTGG AAAGGAGAAG
    GATGTGATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.