Mlh3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mlh3
Entrez Gene ID	217716
Full Name	mutL homolog 3
Synonyms	AV125803,BB126472
General protein information	Preferred Names mutL homolog 3 Names DNA mismatch repair protein Mlh3 mutL 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 D1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006515706.3	XP_006515769.1	DNA mismatch repair protein Mlh3 isoform X1
NM_001304475.1	NP_001291404.1	DNA mismatch repair protein Mlh3
XM_006515706.4	XP_006515769.1	DNA mismatch repair protein Mlh3 isoform X1
XM_006515703.4	XP_006515766.1	DNA mismatch repair protein Mlh3 isoform X1
XM_006515704.4	XP_006515767.1	DNA mismatch repair protein Mlh3 isoform X1
XM_006515703.3	XP_006515766.1	DNA mismatch repair protein Mlh3 isoform X1
XM_006515704.3	XP_006515767.1	DNA mismatch repair protein Mlh3 isoform X1
NM_175337.2	NP_780546.1	DNA mismatch repair protein Mlh3

Pathways from BioSystems

Homologies

Homo sapiens (human)	MLH3	NP_001035197.1
	MLH3	XP_001096911.1
Pan troglodytes (chimpanzee)	MLH3	XP_001158621.1
Mus musculus (house mouse)	Mlh3	NP_780546.1
Saccharomyces cerevisiae (baker's yeast)	MLH3	NP_015161.1
Bos taurus (cattle)	MLH3	XP_003586644.2
Canis lupus familiaris (dog)	MLH3	XP_537511.3
Danio rerio (zebrafish)	mlh3	XP_005162267.1
Eremothecium gossypii	AGOS_AAL093C	NP_982449.1
Rattus norvegicus (Norway rat)	Mlh3	NP_001101513.1
Gallus gallus (chicken)	MLH3	XP_004941860.1
Kluyveromyces lactis	KLLA0C10032g	XP_452646.1
Xenopus tropicalis (tropical clawed frog)	mlh3	NP_001238771.1

Gene Ontology
Bibliography

Related articles in PubMed

Age-Dependent Alterations in Meiotic Recombination Cause Chromosome Segregation Errors in Spermatocytes.
Zelazowski MJ, Sandoval M, Paniker L, Hamilton HM, Han J, Gribbell MA, Kang R, Cole F
Cell171(3)601-614.e13(2017 Oct)

Mammalian CNTD1 is critical for meiotic crossover maturation and deselection of excess precrossover sites.
Holloway JK, Sun X, Yokoo R, Villeneuve AM, Cohen PE
The Journal of cell biology205(5)633-41(2014 Jun)

Antagonistic roles of ubiquitin ligase HEI10 and SUMO ligase RNF212 regulate meiotic recombination.
Qiao H, Prasada Rao HB, Yang Y, Fong JH, Cloutier JM, Deacon DC, Nagel KE, Swartz RK, Strong E, Holloway JK, Cohen PE, Schimenti J, Ward J, Hunter N
Nature genetics46(2)194-9(2014 Feb)

Mismatch repair genes Mlh1 and Mlh3 modify CAG instability in Huntington's disease mice: genome-wide and candidate approaches.
Pinto RM, Dragileva E, Kirby A, Lloret A, Lopez E, St Claire J, Panigrahi GB, Hou C, Holloway K, Gillis T, Guide JR, Cohen PE, Li GM, Pearson CE, Daly MJ, Wheeler VC
PLoS genetics9(10)e1003930(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these data identify Mlh1 and Mlh3 as novel critical genetic modifiers of HTT CAG instability, point to Mlh1 genetic variation as the likely source of the instability difference in B6 and 129 strains
Title: Mismatch repair genes Mlh1 and Mlh3 modify CAG instability in Huntington's disease mice: genome-wide and candidate approaches.

MLH3-independent crossovers in mammalian meiosis,in Mus81-nullizygous mice.
Title: MUS81 generates a subset of MLH1-MLH3-independent crossovers in mammalian meiosis.

Mlh3 nullizygosity significantly increased Apc frameshift mutations and tumor multiplicity.
Title: Novel roles for MLH3 deficiency and TLE6-like amplification in DNA mismatch repair-deficient gastrointestinal tumorigenesis and progression.

These results indicate that approximately 10% of crossovers in the mouse are independent of MLH3, suggesting the existence of alternative crossover pathways in mammals.
Title: Distinct functions of MLH3 at recombination hot spots in the mouse.

Meiotic progression in female mice bearing mutations in genes of the DNA mismatch repair pathway.
Title: Comparative analysis of meiotic progression in female mice bearing mutations in genes of the DNA mismatch repair pathway.

The following Mlh3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mlh3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14539	NM_001304475.1 Latest version!	Mus musculus mutL homolog 3 (Mlh3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OMu14539	NM_175337.2 Latest version!	Mus musculus mutL homolog 3 (Mlh3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OMu47734	XM_006515703.4 Latest version!	Mus musculus mutL homolog 3 (Mlh3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu47734	XM_006515704.4 Latest version!	Mus musculus mutL homolog 3 (Mlh3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu47734	XM_006515706.4 Latest version!	Mus musculus mutL homolog 3 (Mlh3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OMu47734	XM_006515706.3	Mus musculus mutL homolog 3 (Mlh3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu47734	XM_006515703.3	Mus musculus mutL homolog 3 (Mlh3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu47734	XM_006515704.3	Mus musculus mutL homolog 3 (Mlh3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mlh3 ( NM_001304475.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001291404.1 Mus musculus mutL homolog 3 (Mlh3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14539
Clone ID Related Accession (Same CDS sequence)	NM_001304475.1 , NM_175337.2
Accession Version	NM_001304475.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Mus musculus(house mouse)
Product	DNA mismatch repair protein Mlh3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132195.1, BC079861.1 and BE993968.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132195.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGA TGCTGTGGAT GCTGCTGCTG CTGCTGTCAG TGAACCCCTT 
CAGTCTCTGT TTTCAGAATG GAGCAATCCA GTGTTTGCTC GATACCCAGA GGTTGCTGTT 
GATGTCAGCA GTGGCCAGGC TGAGAGCTTA GCCGTTAAAA TTCACAACGT CCTGTATCCC 
TATCGCTTCA CCAAAGAGAT GATTCACTCA GTGAAGGTTC TCCAGCAAGT GGATAACAAG 
TTTATTGCCT GCTTAATGAG CACGAGGATG GATGAGGATG GCCGAACAGG TGGAAACCTG 
TTAGTCCTGG TGGACCAGCA TGCTGCCCAT GAACGCATTC GTTTGGAGCA GCTTATTACT 
GATTCCTATG AGAAACAAGA TCCACAAAGC GCTGGCCGGA AGAAATTATT GTCTTCCACA 
ATAATCCCTC CACTGGCAAT CACCGTGTCA GAGGAACAAA GGAGACTCTT ACGGTCTTAC 
CACAAACATT TAGAAGATCT GGGGCTTGAG TTGCTCTTTC CAGATGCTAG TGATTCTCTG 
ATCCTGGTGG GAAAAGTGCC GCTCTGCTTT GTAGAGAGAG AAGCTAGTGA GCTTCGAAGA 
GGACGCTCTA CTGTGACTAA GAGTATTGTG GAGGAATTAA TTCGAGAACA AGTGGAGCTG 
CTCCAGACCA CAGGAGGTAT CCAAGGGACA CTGCCACTGA CTGTCCAGAA GGTGTTGGCC 
TCCCAGGCCT GCCATGGGGC TATTAAGTTT AATGATCGTC TGAGCCTAGA AGAGAGCTGC 
CGCCTCATCG AAGCTCTGTC CTTGTCCCAG CTGCCATTTC AGTGTGCTCA TGGGAGACCC 
TCAATGCTGC CCTTAGCTGA CCTGGACCAC TTGGAGCAGG AAAAACAGGT TAAACCCAAC 
CTTGCTAAAC TTCGCAAAAT GGTTCGTGCC TGGCATCTCT TTGGAAAAAC AGAGCAGAAC 
CTGCAGCAGC CTATACGTCC TTGTGAGCCT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291404.1
CDS	92..4327
Translation	MIRCLSDDVKTKLRSGLAISSLGQCVEELTLNSIDAEATCVAIRVNMETFQVQVIDNGLGMAGDDVEKVGNRYFTSKCHSVRDLENPAFYGFRGEALASIADMAGAVEISSKKNTTLKTFVKMFQNGKALATHEADLTRPSVGTTVTVYNLFYQFPVRRKSMDPRLEFEKVRQRVEALSLMHPSISFSLRNDVSGSMVLQLPKTKDICSRFCQIYGLGKSQKLREIRFKYKEFEFSGYISSEAHYNKNMQFLFVNRRLVLRTKLHKLIDFLLRKESIICRPKNGSASRQMNSSPRHRSASELHGIYVINVQCPFCEYDVCIEPAKTLIEFQSWDTVLICIQEGVKRFLKQEKLFVELSGEDIKEFNEDNGFSLFGTTLQTHVSTHEKCDQSSFREACNKILDSYEMFNLQSKAVKRIATLENKTRQNPGDSETIRKKTVGSLYTDASDGPCYSKSVESVLQDSNNSAYLEPRVSEEEVAKTSHSGENEKWKKSFLENKTSGRIHETSPKMFSSPIQMHHLLEEREADLEMQTISSTVNVMAANIPQNNDIPSQLEKWKDAPEVGCQPLPFETTLLRVRGTQRKKERRKKEPSSRGRVNVFSYGQVKLCSTGFITHVVQSEHAKSTETEHSFKNYARPGPVSAQETFGKRTHHAIETPDSSDLTSTLSKESSQPPNKRFCRTNTGYGTENKPVATDDNLALFQESCKESHTDRLLPDASSFPWCRYVSDGCRKIDKRGSFKQVVRRKLSLRSQVGSLEKFKRQYGKVSSSLDTEKDNNTEVRTHLDPQNEPDVLLKDKSHLDMSDGCEITTVEHSETCQPLSPILYPEKILFSKEDRLEQMPHLRESPITLEELSHCNRKADVEKSAASLASKLSKLKDSEKEMQTVGMTGHTSELPDSNPSWKDNSQCTRLDLDFCELLKNKLEKIESDMLPMADSATEDGPINKNSELHPNNTTDDTEKPETPLLFPCNDSKISRDSDVLIRTSEQPTGNPDSVGKVIMSQVEDGIGSQGGVCPQGDESKARSCSKNEPNAHCMDWQQHFDVTLGRMVYINRMTGLSTFVAPTDDLHTACTKDLTTVAVDVLLGNDAVDAAAAAVSEPLQSLFSEWSNPVFARYPEVAVDVSSGQAESLAVKIHNVLYPYRFTKEMIHSVKVLQQVDNKFIACLMSTRMDEDGRTGGNLLVLVDQHAAHERIRLEQLITDSYEKQDPQSAGRKKLLSSTIIPPLAITVSEEQRRLLRSYHKHLEDLGLELLFPDASDSLILVGKVPLCFVEREASELRRGRSTVTKSIVEELIREQVELLQTTGGIQGTLPLTVQKVLASQACHGAIKFNDRLSLEESCRLIEALSLSQLPFQCAHGRPSMLPLADLDHLEQEKQVKPNLAKLRKMVRAWHLFGKTEQNLQQPIRPCEPP

Target ORF information:

RefSeq Version	NM_001304475.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mutL homolog 3 (Mlh3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304475.1

ORF Insert Sequence:

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGA TGCTGTGGAT GCTGCTGCTG CTGCTGTCAG TGAACCCCTT 
CAGTCTCTGT TTTCAGAATG GAGCAATCCA GTGTTTGCTC GATACCCAGA GGTTGCTGTT 
GATGTCAGCA GTGGCCAGGC TGAGAGCTTA GCCGTTAAAA TTCACAACGT CCTGTATCCC 
TATCGCTTCA CCAAAGAGAT GATTCACTCA GTGAAGGTTC TCCAGCAAGT GGATAACAAG 
TTTATTGCCT GCTTAATGAG CACGAGGATG GATGAGGATG GCCGAACAGG TGGAAACCTG 
TTAGTCCTGG TGGACCAGCA TGCTGCCCAT GAACGCATTC GTTTGGAGCA GCTTATTACT 
GATTCCTATG AGAAACAAGA TCCACAAAGC GCTGGCCGGA AGAAATTATT GTCTTCCACA 
ATAATCCCTC CACTGGCAAT CACCGTGTCA GAGGAACAAA GGAGACTCTT ACGGTCTTAC 
CACAAACATT TAGAAGATCT GGGGCTTGAG TTGCTCTTTC CAGATGCTAG TGATTCTCTG 
ATCCTGGTGG GAAAAGTGCC GCTCTGCTTT GTAGAGAGAG AAGCTAGTGA GCTTCGAAGA 
GGACGCTCTA CTGTGACTAA GAGTATTGTG GAGGAATTAA TTCGAGAACA AGTGGAGCTG 
CTCCAGACCA CAGGAGGTAT CCAAGGGACA CTGCCACTGA CTGTCCAGAA GGTGTTGGCC 
TCCCAGGCCT GCCATGGGGC TATTAAGTTT AATGATCGTC TGAGCCTAGA AGAGAGCTGC 
CGCCTCATCG AAGCTCTGTC CTTGTCCCAG CTGCCATTTC AGTGTGCTCA TGGGAGACCC 
TCAATGCTGC CCTTAGCTGA CCTGGACCAC TTGGAGCAGG AAAAACAGGT TAAACCCAAC 
CTTGCTAAAC TTCGCAAAAT GGTTCGTGCC TGGCATCTCT TTGGAAAAAC AGAGCAGAAC 
CTGCAGCAGC CTATACGTCC TTGTGAGCCT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlh3 ( NM_175337.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780546.1 Mus musculus mutL homolog 3 (Mlh3), transcript variant 1, mRNA.

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CloneID	OMu14539
Clone ID Related Accession (Same CDS sequence)	NM_001304475.1 , NM_175337.2
Accession Version	NM_175337.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Mus musculus(house mouse)
Product	DNA mismatch repair protein Mlh3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK035887.1, BC079861.1 and BE993968.1. On Jan 28, 2015 this sequence version replaced NM_175337.1. Transcript Variant: This variant (1) is the longer transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC072599.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGA TGCTGTGGAT GCTGCTGCTG CTGCTGTCAG TGAACCCCTT 
CAGTCTCTGT TTTCAGAATG GAGCAATCCA GTGTTTGCTC GATACCCAGA GGTTGCTGTT 
GATGTCAGCA GTGGCCAGGC TGAGAGCTTA GCCGTTAAAA TTCACAACGT CCTGTATCCC 
TATCGCTTCA CCAAAGAGAT GATTCACTCA GTGAAGGTTC TCCAGCAAGT GGATAACAAG 
TTTATTGCCT GCTTAATGAG CACGAGGATG GATGAGGATG GCCGAACAGG TGGAAACCTG 
TTAGTCCTGG TGGACCAGCA TGCTGCCCAT GAACGCATTC GTTTGGAGCA GCTTATTACT 
GATTCCTATG AGAAACAAGA TCCACAAAGC GCTGGCCGGA AGAAATTATT GTCTTCCACA 
ATAATCCCTC CACTGGCAAT CACCGTGTCA GAGGAACAAA GGAGACTCTT ACGGTCTTAC 
CACAAACATT TAGAAGATCT GGGGCTTGAG TTGCTCTTTC CAGATGCTAG TGATTCTCTG 
ATCCTGGTGG GAAAAGTGCC GCTCTGCTTT GTAGAGAGAG AAGCTAGTGA GCTTCGAAGA 
GGACGCTCTA CTGTGACTAA GAGTATTGTG GAGGAATTAA TTCGAGAACA AGTGGAGCTG 
CTCCAGACCA CAGGAGGTAT CCAAGGGACA CTGCCACTGA CTGTCCAGAA GGTGTTGGCC 
TCCCAGGCCT GCCATGGGGC TATTAAGTTT AATGATCGTC TGAGCCTAGA AGAGAGCTGC 
CGCCTCATCG AAGCTCTGTC CTTGTCCCAG CTGCCATTTC AGTGTGCTCA TGGGAGACCC 
TCAATGCTGC CCTTAGCTGA CCTGGACCAC TTGGAGCAGG AAAAACAGGT TAAACCCAAC 
CTTGCTAAAC TTCGCAAAAT GGTTCGTGCC TGGCATCTCT TTGGAAAAAC AGAGCAGAAC 
CTGCAGCAGC CTATACGTCC TTGTGAGCCT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780546.1
CDS	126..4361
Translation	MIRCLSDDVKTKLRSGLAISSLGQCVEELTLNSIDAEATCVAIRVNMETFQVQVIDNGLGMAGDDVEKVGNRYFTSKCHSVRDLENPAFYGFRGEALASIADMAGAVEISSKKNTTLKTFVKMFQNGKALATHEADLTRPSVGTTVTVYNLFYQFPVRRKSMDPRLEFEKVRQRVEALSLMHPSISFSLRNDVSGSMVLQLPKTKDICSRFCQIYGLGKSQKLREIRFKYKEFEFSGYISSEAHYNKNMQFLFVNRRLVLRTKLHKLIDFLLRKESIICRPKNGSASRQMNSSPRHRSASELHGIYVINVQCPFCEYDVCIEPAKTLIEFQSWDTVLICIQEGVKRFLKQEKLFVELSGEDIKEFNEDNGFSLFGTTLQTHVSTHEKCDQSSFREACNKILDSYEMFNLQSKAVKRIATLENKTRQNPGDSETIRKKTVGSLYTDASDGPCYSKSVESVLQDSNNSAYLEPRVSEEEVAKTSHSGENEKWKKSFLENKTSGRIHETSPKMFSSPIQMHHLLEEREADLEMQTISSTVNVMAANIPQNNDIPSQLEKWKDAPEVGCQPLPFETTLLRVRGTQRKKERRKKEPSSRGRVNVFSYGQVKLCSTGFITHVVQSEHAKSTETEHSFKNYARPGPVSAQETFGKRTHHAIETPDSSDLTSTLSKESSQPPNKRFCRTNTGYGTENKPVATDDNLALFQESCKESHTDRLLPDASSFPWCRYVSDGCRKIDKRGSFKQVVRRKLSLRSQVGSLEKFKRQYGKVSSSLDTEKDNNTEVRTHLDPQNEPDVLLKDKSHLDMSDGCEITTVEHSETCQPLSPILYPEKILFSKEDRLEQMPHLRESPITLEELSHCNRKADVEKSAASLASKLSKLKDSEKEMQTVGMTGHTSELPDSNPSWKDNSQCTRLDLDFCELLKNKLEKIESDMLPMADSATEDGPINKNSELHPNNTTDDTEKPETPLLFPCNDSKISRDSDVLIRTSEQPTGNPDSVGKVIMSQVEDGIGSQGGVCPQGDESKARSCSKNEPNAHCMDWQQHFDVTLGRMVYINRMTGLSTFVAPTDDLHTACTKDLTTVAVDVLLGNDAVDAAAAAVSEPLQSLFSEWSNPVFARYPEVAVDVSSGQAESLAVKIHNVLYPYRFTKEMIHSVKVLQQVDNKFIACLMSTRMDEDGRTGGNLLVLVDQHAAHERIRLEQLITDSYEKQDPQSAGRKKLLSSTIIPPLAITVSEEQRRLLRSYHKHLEDLGLELLFPDASDSLILVGKVPLCFVEREASELRRGRSTVTKSIVEELIREQVELLQTTGGIQGTLPLTVQKVLASQACHGAIKFNDRLSLEESCRLIEALSLSQLPFQCAHGRPSMLPLADLDHLEQEKQVKPNLAKLRKMVRAWHLFGKTEQNLQQPIRPCEPP

Target ORF information:

RefSeq Version	NM_175337.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mutL homolog 3 (Mlh3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175337.2

ORF Insert Sequence:

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGA TGCTGTGGAT GCTGCTGCTG CTGCTGTCAG TGAACCCCTT 
CAGTCTCTGT TTTCAGAATG GAGCAATCCA GTGTTTGCTC GATACCCAGA GGTTGCTGTT 
GATGTCAGCA GTGGCCAGGC TGAGAGCTTA GCCGTTAAAA TTCACAACGT CCTGTATCCC 
TATCGCTTCA CCAAAGAGAT GATTCACTCA GTGAAGGTTC TCCAGCAAGT GGATAACAAG 
TTTATTGCCT GCTTAATGAG CACGAGGATG GATGAGGATG GCCGAACAGG TGGAAACCTG 
TTAGTCCTGG TGGACCAGCA TGCTGCCCAT GAACGCATTC GTTTGGAGCA GCTTATTACT 
GATTCCTATG AGAAACAAGA TCCACAAAGC GCTGGCCGGA AGAAATTATT GTCTTCCACA 
ATAATCCCTC CACTGGCAAT CACCGTGTCA GAGGAACAAA GGAGACTCTT ACGGTCTTAC 
CACAAACATT TAGAAGATCT GGGGCTTGAG TTGCTCTTTC CAGATGCTAG TGATTCTCTG 
ATCCTGGTGG GAAAAGTGCC GCTCTGCTTT GTAGAGAGAG AAGCTAGTGA GCTTCGAAGA 
GGACGCTCTA CTGTGACTAA GAGTATTGTG GAGGAATTAA TTCGAGAACA AGTGGAGCTG 
CTCCAGACCA CAGGAGGTAT CCAAGGGACA CTGCCACTGA CTGTCCAGAA GGTGTTGGCC 
TCCCAGGCCT GCCATGGGGC TATTAAGTTT AATGATCGTC TGAGCCTAGA AGAGAGCTGC 
CGCCTCATCG AAGCTCTGTC CTTGTCCCAG CTGCCATTTC AGTGTGCTCA TGGGAGACCC 
TCAATGCTGC CCTTAGCTGA CCTGGACCAC TTGGAGCAGG AAAAACAGGT TAAACCCAAC 
CTTGCTAAAC TTCGCAAAAT GGTTCGTGCC TGGCATCTCT TTGGAAAAAC AGAGCAGAAC 
CTGCAGCAGC CTATACGTCC TTGTGAGCCT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlh3 ( XM_006515706.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515769.1 Mus musculus mutL homolog 3 (Mlh3), transcript variant X3, mRNA.

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CloneID	OMu47734
Clone ID Related Accession (Same CDS sequence)	XM_006515706.3 , XM_006515706.4 , XM_006515703.4 , XM_006515704.4 , XM_006515703.3 , XM_006515704.3
Accession Version	XM_006515706.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA mismatch repair protein Mlh3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515706.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGG GGCTCAGTAC AGGTGTCATC CATTTAGAAG CGATCTTGTT 
CTTCCTTTCC TGCCAAGAGC TCAGGAAGAG AGGACCATGC TGAGGCACAA CAGCAATGCT 
GTGGATGCTG CTGCTGCTGC TGTCAGTGAA CCCCTTCAGT CTCTGTTTTC AGAATGGAGC 
AATCCAGTGT TTGCTCGATA CCCAGAGGTT GCTGTTGATG TCAGCAGTGG CCAGGCTGAG 
AGCTTAGCCG TTAAAATTCA CAACGTCCTG TATCCCTATC GCTTCACCAA AGAGATGATT 
CACTCAGTGA AGGTTCTCCA GCAAGTGGAT AACAAGTTTA TTGCCTGCTT AATGAGCACG 
AGGATGGATG AGGATGGCCG AACAGGTGGA AACCTGTTAG TCCTGGTGGA CCAGCATGCT 
GCCCATGAAC GCATTCGTTT GGAGCAGCTT ATTACTGATT CCTATGAGAA ACAAGATCCA 
CAAAGCGCTG GCCGGAAGAA ATTATTGTCT TCCACAATAA TCCCTCCACT GGCAATCACC 
GTGTCAGAGG AACAAAGGAG ACTCTTACGG TCTTACCACA AACATTTAGA AGATCTGGGG 
CTTGAGTTGC TCTTTCCAGA TGCTAGTGAT TCTCTGATCC TGGTGGGAAA AGTGCCGCTC 
TGCTTTGTAG AGAGAGAAGC TAGTGAGCTT CGAAGAGGAC GCTCTACTGT GACTAAGAGT 
ATTGTGGAGG AATTAATTCG AGAACAAGTG GAGCTGCTCC AGACCACAGG AGGTATCCAA 
GGGACACTGC CACTGACTGT CCAGAAGGTG TTGGCCTCCC AGGCCTGCCA TGGGGCTATT 
AAGTTTAATG ATCGTCTGAG CCTAGAAGAG AGCTGCCGCC TCATCGAAGC TCTGTCCTTG 
TCCCAGCTGC CATTTCAGTG TGCTCATGGG AGACCCTCAA TGCTGCCCTT AGCTGACCTG 
GACCACTTGG AGCAGGAAAA ACAGGTTAAA CCCAACCTTG CTAAACTTCG CAAAATGGTT 
CGTGCCTGGC ATCTCTTTGG AAAAACAGAG CAGAACCTGC AGCAGCCTAT ACGTCCTTGT 
GAGCCTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515769.1
CDS	139..4470
Translation	MIRCLSDDVKTKLRSGLAISSLGQCVEELTLNSIDAEATCVAIRVNMETFQVQVIDNGLGMAGDDVEKVGNRYFTSKCHSVRDLENPAFYGFRGEALASIADMAGAVEISSKKNTTLKTFVKMFQNGKALATHEADLTRPSVGTTVTVYNLFYQFPVRRKSMDPRLEFEKVRQRVEALSLMHPSISFSLRNDVSGSMVLQLPKTKDICSRFCQIYGLGKSQKLREIRFKYKEFEFSGYISSEAHYNKNMQFLFVNRRLVLRTKLHKLIDFLLRKESIICRPKNGSASRQMNSSPRHRSASELHGIYVINVQCPFCEYDVCIEPAKTLIEFQSWDTVLICIQEGVKRFLKQEKLFVELSGEDIKEFNEDNGFSLFGTTLQTHVSTHEKCDQSSFREACNKILDSYEMFNLQSKAVKRIATLENKTRQNPGDSETIRKKTVGSLYTDASDGPCYSKSVESVLQDSNNSAYLEPRVSEEEVAKTSHSGENEKWKKSFLENKTSGRIHETSPKMFSSPIQMHHLLEEREADLEMQTISSTVNVMAANIPQNNDIPSQLEKWKDAPEVGCQPLPFETTLLRVRGTQRKKERRKKEPSSRGRVNVFSYGQVKLCSTGFITHVVQSEHAKSTETEHSFKNYARPGPVSAQETFGKRTHHAIETPDSSDLTSTLSKESSQPPNKRFCRTNTGYGTENKPVATDDNLALFQESCKESHTDRLLPDASSFPWCRYVSDGCRKIDKRGSFKQVVRRKLSLRSQVGSLEKFKRQYGKVSSSLDTEKDNNTEVRTHLDPQNEPDVLLKDKSHLDMSDGCEITTVEHSETCQPLSPILYPEKILFSKEDRLEQMPHLRESPITLEELSHCNRKADVEKSAASLASKLSKLKDSEKEMQTVGMTGHTSELPDSNPSWKDNSQCTRLDLDFCELLKNKLEKIESDMLPMADSATEDGPINKNSELHPNNTTDDTEKPETPLLFPCNDSKISRDSDVLIRTSEQPTGNPDSVGKVIMSQVEDGIGSQGGVCPQGDESKARSCSKNEPNAHCMDWQQHFDVTLGRMVYINRMTGLSTFVAPTDDLHTACTKDLTTVAVDVLLGNGAQYRCHPFRSDLVLPFLPRAQEERTMLRHNSNAVDAAAAAVSEPLQSLFSEWSNPVFARYPEVAVDVSSGQAESLAVKIHNVLYPYRFTKEMIHSVKVLQQVDNKFIACLMSTRMDEDGRTGGNLLVLVDQHAAHERIRLEQLITDSYEKQDPQSAGRKKLLSSTIIPPLAITVSEEQRRLLRSYHKHLEDLGLELLFPDASDSLILVGKVPLCFVEREASELRRGRSTVTKSIVEELIREQVELLQTTGGIQGTLPLTVQKVLASQACHGAIKFNDRLSLEESCRLIEALSLSQLPFQCAHGRPSMLPLADLDHLEQEKQVKPNLAKLRKMVRAWHLFGKTEQNLQQPIRPCEPP

Target ORF information:

RefSeq Version	XM_006515706.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mutL homolog 3 (Mlh3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515706.4

ORF Insert Sequence:

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGG GGCTCAGTAC AGGTGTCATC CATTTAGAAG CGATCTTGTT 
CTTCCTTTCC TGCCAAGAGC TCAGGAAGAG AGGACCATGC TGAGGCACAA CAGCAATGCT 
GTGGATGCTG CTGCTGCTGC TGTCAGTGAA CCCCTTCAGT CTCTGTTTTC AGAATGGAGC 
AATCCAGTGT TTGCTCGATA CCCAGAGGTT GCTGTTGATG TCAGCAGTGG CCAGGCTGAG 
AGCTTAGCCG TTAAAATTCA CAACGTCCTG TATCCCTATC GCTTCACCAA AGAGATGATT 
CACTCAGTGA AGGTTCTCCA GCAAGTGGAT AACAAGTTTA TTGCCTGCTT AATGAGCACG 
AGGATGGATG AGGATGGCCG AACAGGTGGA AACCTGTTAG TCCTGGTGGA CCAGCATGCT 
GCCCATGAAC GCATTCGTTT GGAGCAGCTT ATTACTGATT CCTATGAGAA ACAAGATCCA 
CAAAGCGCTG GCCGGAAGAA ATTATTGTCT TCCACAATAA TCCCTCCACT GGCAATCACC 
GTGTCAGAGG AACAAAGGAG ACTCTTACGG TCTTACCACA AACATTTAGA AGATCTGGGG 
CTTGAGTTGC TCTTTCCAGA TGCTAGTGAT TCTCTGATCC TGGTGGGAAA AGTGCCGCTC 
TGCTTTGTAG AGAGAGAAGC TAGTGAGCTT CGAAGAGGAC GCTCTACTGT GACTAAGAGT 
ATTGTGGAGG AATTAATTCG AGAACAAGTG GAGCTGCTCC AGACCACAGG AGGTATCCAA 
GGGACACTGC CACTGACTGT CCAGAAGGTG TTGGCCTCCC AGGCCTGCCA TGGGGCTATT 
AAGTTTAATG ATCGTCTGAG CCTAGAAGAG AGCTGCCGCC TCATCGAAGC TCTGTCCTTG 
TCCCAGCTGC CATTTCAGTG TGCTCATGGG AGACCCTCAA TGCTGCCCTT AGCTGACCTG 
GACCACTTGG AGCAGGAAAA ACAGGTTAAA CCCAACCTTG CTAAACTTCG CAAAATGGTT 
CGTGCCTGGC ATCTCTTTGG AAAAACAGAG CAGAACCTGC AGCAGCCTAT ACGTCCTTGT 
GAGCCTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlh3 ( XM_006515703.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515766.1 Mus musculus mutL homolog 3 (Mlh3), transcript variant X1, mRNA.

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CloneID	OMu47734
Clone ID Related Accession (Same CDS sequence)	XM_006515706.3 , XM_006515706.4 , XM_006515703.4 , XM_006515704.4 , XM_006515703.3 , XM_006515704.3
Accession Version	XM_006515703.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA mismatch repair protein Mlh3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515703.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGG GGCTCAGTAC AGGTGTCATC CATTTAGAAG CGATCTTGTT 
CTTCCTTTCC TGCCAAGAGC TCAGGAAGAG AGGACCATGC TGAGGCACAA CAGCAATGCT 
GTGGATGCTG CTGCTGCTGC TGTCAGTGAA CCCCTTCAGT CTCTGTTTTC AGAATGGAGC 
AATCCAGTGT TTGCTCGATA CCCAGAGGTT GCTGTTGATG TCAGCAGTGG CCAGGCTGAG 
AGCTTAGCCG TTAAAATTCA CAACGTCCTG TATCCCTATC GCTTCACCAA AGAGATGATT 
CACTCAGTGA AGGTTCTCCA GCAAGTGGAT AACAAGTTTA TTGCCTGCTT AATGAGCACG 
AGGATGGATG AGGATGGCCG AACAGGTGGA AACCTGTTAG TCCTGGTGGA CCAGCATGCT 
GCCCATGAAC GCATTCGTTT GGAGCAGCTT ATTACTGATT CCTATGAGAA ACAAGATCCA 
CAAAGCGCTG GCCGGAAGAA ATTATTGTCT TCCACAATAA TCCCTCCACT GGCAATCACC 
GTGTCAGAGG AACAAAGGAG ACTCTTACGG TCTTACCACA AACATTTAGA AGATCTGGGG 
CTTGAGTTGC TCTTTCCAGA TGCTAGTGAT TCTCTGATCC TGGTGGGAAA AGTGCCGCTC 
TGCTTTGTAG AGAGAGAAGC TAGTGAGCTT CGAAGAGGAC GCTCTACTGT GACTAAGAGT 
ATTGTGGAGG AATTAATTCG AGAACAAGTG GAGCTGCTCC AGACCACAGG AGGTATCCAA 
GGGACACTGC CACTGACTGT CCAGAAGGTG TTGGCCTCCC AGGCCTGCCA TGGGGCTATT 
AAGTTTAATG ATCGTCTGAG CCTAGAAGAG AGCTGCCGCC TCATCGAAGC TCTGTCCTTG 
TCCCAGCTGC CATTTCAGTG TGCTCATGGG AGACCCTCAA TGCTGCCCTT AGCTGACCTG 
GACCACTTGG AGCAGGAAAA ACAGGTTAAA CCCAACCTTG CTAAACTTCG CAAAATGGTT 
CGTGCCTGGC ATCTCTTTGG AAAAACAGAG CAGAACCTGC AGCAGCCTAT ACGTCCTTGT 
GAGCCTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515766.1
CDS	174..4505
Translation	MIRCLSDDVKTKLRSGLAISSLGQCVEELTLNSIDAEATCVAIRVNMETFQVQVIDNGLGMAGDDVEKVGNRYFTSKCHSVRDLENPAFYGFRGEALASIADMAGAVEISSKKNTTLKTFVKMFQNGKALATHEADLTRPSVGTTVTVYNLFYQFPVRRKSMDPRLEFEKVRQRVEALSLMHPSISFSLRNDVSGSMVLQLPKTKDICSRFCQIYGLGKSQKLREIRFKYKEFEFSGYISSEAHYNKNMQFLFVNRRLVLRTKLHKLIDFLLRKESIICRPKNGSASRQMNSSPRHRSASELHGIYVINVQCPFCEYDVCIEPAKTLIEFQSWDTVLICIQEGVKRFLKQEKLFVELSGEDIKEFNEDNGFSLFGTTLQTHVSTHEKCDQSSFREACNKILDSYEMFNLQSKAVKRIATLENKTRQNPGDSETIRKKTVGSLYTDASDGPCYSKSVESVLQDSNNSAYLEPRVSEEEVAKTSHSGENEKWKKSFLENKTSGRIHETSPKMFSSPIQMHHLLEEREADLEMQTISSTVNVMAANIPQNNDIPSQLEKWKDAPEVGCQPLPFETTLLRVRGTQRKKERRKKEPSSRGRVNVFSYGQVKLCSTGFITHVVQSEHAKSTETEHSFKNYARPGPVSAQETFGKRTHHAIETPDSSDLTSTLSKESSQPPNKRFCRTNTGYGTENKPVATDDNLALFQESCKESHTDRLLPDASSFPWCRYVSDGCRKIDKRGSFKQVVRRKLSLRSQVGSLEKFKRQYGKVSSSLDTEKDNNTEVRTHLDPQNEPDVLLKDKSHLDMSDGCEITTVEHSETCQPLSPILYPEKILFSKEDRLEQMPHLRESPITLEELSHCNRKADVEKSAASLASKLSKLKDSEKEMQTVGMTGHTSELPDSNPSWKDNSQCTRLDLDFCELLKNKLEKIESDMLPMADSATEDGPINKNSELHPNNTTDDTEKPETPLLFPCNDSKISRDSDVLIRTSEQPTGNPDSVGKVIMSQVEDGIGSQGGVCPQGDESKARSCSKNEPNAHCMDWQQHFDVTLGRMVYINRMTGLSTFVAPTDDLHTACTKDLTTVAVDVLLGNGAQYRCHPFRSDLVLPFLPRAQEERTMLRHNSNAVDAAAAAVSEPLQSLFSEWSNPVFARYPEVAVDVSSGQAESLAVKIHNVLYPYRFTKEMIHSVKVLQQVDNKFIACLMSTRMDEDGRTGGNLLVLVDQHAAHERIRLEQLITDSYEKQDPQSAGRKKLLSSTIIPPLAITVSEEQRRLLRSYHKHLEDLGLELLFPDASDSLILVGKVPLCFVEREASELRRGRSTVTKSIVEELIREQVELLQTTGGIQGTLPLTVQKVLASQACHGAIKFNDRLSLEESCRLIEALSLSQLPFQCAHGRPSMLPLADLDHLEQEKQVKPNLAKLRKMVRAWHLFGKTEQNLQQPIRPCEPP

Target ORF information:

RefSeq Version	XM_006515703.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mutL homolog 3 (Mlh3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515703.4

ORF Insert Sequence:

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGG GGCTCAGTAC AGGTGTCATC CATTTAGAAG CGATCTTGTT 
CTTCCTTTCC TGCCAAGAGC TCAGGAAGAG AGGACCATGC TGAGGCACAA CAGCAATGCT 
GTGGATGCTG CTGCTGCTGC TGTCAGTGAA CCCCTTCAGT CTCTGTTTTC AGAATGGAGC 
AATCCAGTGT TTGCTCGATA CCCAGAGGTT GCTGTTGATG TCAGCAGTGG CCAGGCTGAG 
AGCTTAGCCG TTAAAATTCA CAACGTCCTG TATCCCTATC GCTTCACCAA AGAGATGATT 
CACTCAGTGA AGGTTCTCCA GCAAGTGGAT AACAAGTTTA TTGCCTGCTT AATGAGCACG 
AGGATGGATG AGGATGGCCG AACAGGTGGA AACCTGTTAG TCCTGGTGGA CCAGCATGCT 
GCCCATGAAC GCATTCGTTT GGAGCAGCTT ATTACTGATT CCTATGAGAA ACAAGATCCA 
CAAAGCGCTG GCCGGAAGAA ATTATTGTCT TCCACAATAA TCCCTCCACT GGCAATCACC 
GTGTCAGAGG AACAAAGGAG ACTCTTACGG TCTTACCACA AACATTTAGA AGATCTGGGG 
CTTGAGTTGC TCTTTCCAGA TGCTAGTGAT TCTCTGATCC TGGTGGGAAA AGTGCCGCTC 
TGCTTTGTAG AGAGAGAAGC TAGTGAGCTT CGAAGAGGAC GCTCTACTGT GACTAAGAGT 
ATTGTGGAGG AATTAATTCG AGAACAAGTG GAGCTGCTCC AGACCACAGG AGGTATCCAA 
GGGACACTGC CACTGACTGT CCAGAAGGTG TTGGCCTCCC AGGCCTGCCA TGGGGCTATT 
AAGTTTAATG ATCGTCTGAG CCTAGAAGAG AGCTGCCGCC TCATCGAAGC TCTGTCCTTG 
TCCCAGCTGC CATTTCAGTG TGCTCATGGG AGACCCTCAA TGCTGCCCTT AGCTGACCTG 
GACCACTTGG AGCAGGAAAA ACAGGTTAAA CCCAACCTTG CTAAACTTCG CAAAATGGTT 
CGTGCCTGGC ATCTCTTTGG AAAAACAGAG CAGAACCTGC AGCAGCCTAT ACGTCCTTGT 
GAGCCTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlh3 ( XM_006515704.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515767.1 Mus musculus mutL homolog 3 (Mlh3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu47734
Clone ID Related Accession (Same CDS sequence)	XM_006515706.3 , XM_006515706.4 , XM_006515703.4 , XM_006515704.4 , XM_006515703.3 , XM_006515704.3
Accession Version	XM_006515704.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA mismatch repair protein Mlh3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515704.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGG GGCTCAGTAC AGGTGTCATC CATTTAGAAG CGATCTTGTT 
CTTCCTTTCC TGCCAAGAGC TCAGGAAGAG AGGACCATGC TGAGGCACAA CAGCAATGCT 
GTGGATGCTG CTGCTGCTGC TGTCAGTGAA CCCCTTCAGT CTCTGTTTTC AGAATGGAGC 
AATCCAGTGT TTGCTCGATA CCCAGAGGTT GCTGTTGATG TCAGCAGTGG CCAGGCTGAG 
AGCTTAGCCG TTAAAATTCA CAACGTCCTG TATCCCTATC GCTTCACCAA AGAGATGATT 
CACTCAGTGA AGGTTCTCCA GCAAGTGGAT AACAAGTTTA TTGCCTGCTT AATGAGCACG 
AGGATGGATG AGGATGGCCG AACAGGTGGA AACCTGTTAG TCCTGGTGGA CCAGCATGCT 
GCCCATGAAC GCATTCGTTT GGAGCAGCTT ATTACTGATT CCTATGAGAA ACAAGATCCA 
CAAAGCGCTG GCCGGAAGAA ATTATTGTCT TCCACAATAA TCCCTCCACT GGCAATCACC 
GTGTCAGAGG AACAAAGGAG ACTCTTACGG TCTTACCACA AACATTTAGA AGATCTGGGG 
CTTGAGTTGC TCTTTCCAGA TGCTAGTGAT TCTCTGATCC TGGTGGGAAA AGTGCCGCTC 
TGCTTTGTAG AGAGAGAAGC TAGTGAGCTT CGAAGAGGAC GCTCTACTGT GACTAAGAGT 
ATTGTGGAGG AATTAATTCG AGAACAAGTG GAGCTGCTCC AGACCACAGG AGGTATCCAA 
GGGACACTGC CACTGACTGT CCAGAAGGTG TTGGCCTCCC AGGCCTGCCA TGGGGCTATT 
AAGTTTAATG ATCGTCTGAG CCTAGAAGAG AGCTGCCGCC TCATCGAAGC TCTGTCCTTG 
TCCCAGCTGC CATTTCAGTG TGCTCATGGG AGACCCTCAA TGCTGCCCTT AGCTGACCTG 
GACCACTTGG AGCAGGAAAA ACAGGTTAAA CCCAACCTTG CTAAACTTCG CAAAATGGTT 
CGTGCCTGGC ATCTCTTTGG AAAAACAGAG CAGAACCTGC AGCAGCCTAT ACGTCCTTGT 
GAGCCTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515767.1
CDS	238..4569
Translation	MIRCLSDDVKTKLRSGLAISSLGQCVEELTLNSIDAEATCVAIRVNMETFQVQVIDNGLGMAGDDVEKVGNRYFTSKCHSVRDLENPAFYGFRGEALASIADMAGAVEISSKKNTTLKTFVKMFQNGKALATHEADLTRPSVGTTVTVYNLFYQFPVRRKSMDPRLEFEKVRQRVEALSLMHPSISFSLRNDVSGSMVLQLPKTKDICSRFCQIYGLGKSQKLREIRFKYKEFEFSGYISSEAHYNKNMQFLFVNRRLVLRTKLHKLIDFLLRKESIICRPKNGSASRQMNSSPRHRSASELHGIYVINVQCPFCEYDVCIEPAKTLIEFQSWDTVLICIQEGVKRFLKQEKLFVELSGEDIKEFNEDNGFSLFGTTLQTHVSTHEKCDQSSFREACNKILDSYEMFNLQSKAVKRIATLENKTRQNPGDSETIRKKTVGSLYTDASDGPCYSKSVESVLQDSNNSAYLEPRVSEEEVAKTSHSGENEKWKKSFLENKTSGRIHETSPKMFSSPIQMHHLLEEREADLEMQTISSTVNVMAANIPQNNDIPSQLEKWKDAPEVGCQPLPFETTLLRVRGTQRKKERRKKEPSSRGRVNVFSYGQVKLCSTGFITHVVQSEHAKSTETEHSFKNYARPGPVSAQETFGKRTHHAIETPDSSDLTSTLSKESSQPPNKRFCRTNTGYGTENKPVATDDNLALFQESCKESHTDRLLPDASSFPWCRYVSDGCRKIDKRGSFKQVVRRKLSLRSQVGSLEKFKRQYGKVSSSLDTEKDNNTEVRTHLDPQNEPDVLLKDKSHLDMSDGCEITTVEHSETCQPLSPILYPEKILFSKEDRLEQMPHLRESPITLEELSHCNRKADVEKSAASLASKLSKLKDSEKEMQTVGMTGHTSELPDSNPSWKDNSQCTRLDLDFCELLKNKLEKIESDMLPMADSATEDGPINKNSELHPNNTTDDTEKPETPLLFPCNDSKISRDSDVLIRTSEQPTGNPDSVGKVIMSQVEDGIGSQGGVCPQGDESKARSCSKNEPNAHCMDWQQHFDVTLGRMVYINRMTGLSTFVAPTDDLHTACTKDLTTVAVDVLLGNGAQYRCHPFRSDLVLPFLPRAQEERTMLRHNSNAVDAAAAAVSEPLQSLFSEWSNPVFARYPEVAVDVSSGQAESLAVKIHNVLYPYRFTKEMIHSVKVLQQVDNKFIACLMSTRMDEDGRTGGNLLVLVDQHAAHERIRLEQLITDSYEKQDPQSAGRKKLLSSTIIPPLAITVSEEQRRLLRSYHKHLEDLGLELLFPDASDSLILVGKVPLCFVEREASELRRGRSTVTKSIVEELIREQVELLQTTGGIQGTLPLTVQKVLASQACHGAIKFNDRLSLEESCRLIEALSLSQLPFQCAHGRPSMLPLADLDHLEQEKQVKPNLAKLRKMVRAWHLFGKTEQNLQQPIRPCEPP

Target ORF information:

RefSeq Version	XM_006515704.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mutL homolog 3 (Mlh3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515704.4

ORF Insert Sequence:

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGG GGCTCAGTAC AGGTGTCATC CATTTAGAAG CGATCTTGTT 
CTTCCTTTCC TGCCAAGAGC TCAGGAAGAG AGGACCATGC TGAGGCACAA CAGCAATGCT 
GTGGATGCTG CTGCTGCTGC TGTCAGTGAA CCCCTTCAGT CTCTGTTTTC AGAATGGAGC 
AATCCAGTGT TTGCTCGATA CCCAGAGGTT GCTGTTGATG TCAGCAGTGG CCAGGCTGAG 
AGCTTAGCCG TTAAAATTCA CAACGTCCTG TATCCCTATC GCTTCACCAA AGAGATGATT 
CACTCAGTGA AGGTTCTCCA GCAAGTGGAT AACAAGTTTA TTGCCTGCTT AATGAGCACG 
AGGATGGATG AGGATGGCCG AACAGGTGGA AACCTGTTAG TCCTGGTGGA CCAGCATGCT 
GCCCATGAAC GCATTCGTTT GGAGCAGCTT ATTACTGATT CCTATGAGAA ACAAGATCCA 
CAAAGCGCTG GCCGGAAGAA ATTATTGTCT TCCACAATAA TCCCTCCACT GGCAATCACC 
GTGTCAGAGG AACAAAGGAG ACTCTTACGG TCTTACCACA AACATTTAGA AGATCTGGGG 
CTTGAGTTGC TCTTTCCAGA TGCTAGTGAT TCTCTGATCC TGGTGGGAAA AGTGCCGCTC 
TGCTTTGTAG AGAGAGAAGC TAGTGAGCTT CGAAGAGGAC GCTCTACTGT GACTAAGAGT 
ATTGTGGAGG AATTAATTCG AGAACAAGTG GAGCTGCTCC AGACCACAGG AGGTATCCAA 
GGGACACTGC CACTGACTGT CCAGAAGGTG TTGGCCTCCC AGGCCTGCCA TGGGGCTATT 
AAGTTTAATG ATCGTCTGAG CCTAGAAGAG AGCTGCCGCC TCATCGAAGC TCTGTCCTTG 
TCCCAGCTGC CATTTCAGTG TGCTCATGGG AGACCCTCAA TGCTGCCCTT AGCTGACCTG 
GACCACTTGG AGCAGGAAAA ACAGGTTAAA CCCAACCTTG CTAAACTTCG CAAAATGGTT 
CGTGCCTGGC ATCTCTTTGG AAAAACAGAG CAGAACCTGC AGCAGCCTAT ACGTCCTTGT 
GAGCCTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlh3 ( XM_006515706.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515769.1 Mus musculus mutL homolog 3 (Mlh3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu47734
Clone ID Related Accession (Same CDS sequence)	XM_006515706.3 , XM_006515706.4 , XM_006515703.4 , XM_006515704.4 , XM_006515703.3 , XM_006515704.3
Accession Version	XM_006515706.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA mismatch repair protein Mlh3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGG GGCTCAGTAC AGGTGTCATC CATTTAGAAG CGATCTTGTT 
CTTCCTTTCC TGCCAAGAGC TCAGGAAGAG AGGACCATGC TGAGGCACAA CAGCAATGCT 
GTGGATGCTG CTGCTGCTGC TGTCAGTGAA CCCCTTCAGT CTCTGTTTTC AGAATGGAGC 
AATCCAGTGT TTGCTCGATA CCCAGAGGTT GCTGTTGATG TCAGCAGTGG CCAGGCTGAG 
AGCTTAGCCG TTAAAATTCA CAACGTCCTG TATCCCTATC GCTTCACCAA AGAGATGATT 
CACTCAGTGA AGGTTCTCCA GCAAGTGGAT AACAAGTTTA TTGCCTGCTT AATGAGCACG 
AGGATGGATG AGGATGGCCG AACAGGTGGA AACCTGTTAG TCCTGGTGGA CCAGCATGCT 
GCCCATGAAC GCATTCGTTT GGAGCAGCTT ATTACTGATT CCTATGAGAA ACAAGATCCA 
CAAAGCGCTG GCCGGAAGAA ATTATTGTCT TCCACAATAA TCCCTCCACT GGCAATCACC 
GTGTCAGAGG AACAAAGGAG ACTCTTACGG TCTTACCACA AACATTTAGA AGATCTGGGG 
CTTGAGTTGC TCTTTCCAGA TGCTAGTGAT TCTCTGATCC TGGTGGGAAA AGTGCCGCTC 
TGCTTTGTAG AGAGAGAAGC TAGTGAGCTT CGAAGAGGAC GCTCTACTGT GACTAAGAGT 
ATTGTGGAGG AATTAATTCG AGAACAAGTG GAGCTGCTCC AGACCACAGG AGGTATCCAA 
GGGACACTGC CACTGACTGT CCAGAAGGTG TTGGCCTCCC AGGCCTGCCA TGGGGCTATT 
AAGTTTAATG ATCGTCTGAG CCTAGAAGAG AGCTGCCGCC TCATCGAAGC TCTGTCCTTG 
TCCCAGCTGC CATTTCAGTG TGCTCATGGG AGACCCTCAA TGCTGCCCTT AGCTGACCTG 
GACCACTTGG AGCAGGAAAA ACAGGTTAAA CCCAACCTTG CTAAACTTCG CAAAATGGTT 
CGTGCCTGGC ATCTCTTTGG AAAAACAGAG CAGAACCTGC AGCAGCCTAT ACGTCCTTGT 
GAGCCTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515769.1
CDS	138..4469
Translation	MIRCLSDDVKTKLRSGLAISSLGQCVEELTLNSIDAEATCVAIRVNMETFQVQVIDNGLGMAGDDVEKVGNRYFTSKCHSVRDLENPAFYGFRGEALASIADMAGAVEISSKKNTTLKTFVKMFQNGKALATHEADLTRPSVGTTVTVYNLFYQFPVRRKSMDPRLEFEKVRQRVEALSLMHPSISFSLRNDVSGSMVLQLPKTKDICSRFCQIYGLGKSQKLREIRFKYKEFEFSGYISSEAHYNKNMQFLFVNRRLVLRTKLHKLIDFLLRKESIICRPKNGSASRQMNSSPRHRSASELHGIYVINVQCPFCEYDVCIEPAKTLIEFQSWDTVLICIQEGVKRFLKQEKLFVELSGEDIKEFNEDNGFSLFGTTLQTHVSTHEKCDQSSFREACNKILDSYEMFNLQSKAVKRIATLENKTRQNPGDSETIRKKTVGSLYTDASDGPCYSKSVESVLQDSNNSAYLEPRVSEEEVAKTSHSGENEKWKKSFLENKTSGRIHETSPKMFSSPIQMHHLLEEREADLEMQTISSTVNVMAANIPQNNDIPSQLEKWKDAPEVGCQPLPFETTLLRVRGTQRKKERRKKEPSSRGRVNVFSYGQVKLCSTGFITHVVQSEHAKSTETEHSFKNYARPGPVSAQETFGKRTHHAIETPDSSDLTSTLSKESSQPPNKRFCRTNTGYGTENKPVATDDNLALFQESCKESHTDRLLPDASSFPWCRYVSDGCRKIDKRGSFKQVVRRKLSLRSQVGSLEKFKRQYGKVSSSLDTEKDNNTEVRTHLDPQNEPDVLLKDKSHLDMSDGCEITTVEHSETCQPLSPILYPEKILFSKEDRLEQMPHLRESPITLEELSHCNRKADVEKSAASLASKLSKLKDSEKEMQTVGMTGHTSELPDSNPSWKDNSQCTRLDLDFCELLKNKLEKIESDMLPMADSATEDGPINKNSELHPNNTTDDTEKPETPLLFPCNDSKISRDSDVLIRTSEQPTGNPDSVGKVIMSQVEDGIGSQGGVCPQGDESKARSCSKNEPNAHCMDWQQHFDVTLGRMVYINRMTGLSTFVAPTDDLHTACTKDLTTVAVDVLLGNGAQYRCHPFRSDLVLPFLPRAQEERTMLRHNSNAVDAAAAAVSEPLQSLFSEWSNPVFARYPEVAVDVSSGQAESLAVKIHNVLYPYRFTKEMIHSVKVLQQVDNKFIACLMSTRMDEDGRTGGNLLVLVDQHAAHERIRLEQLITDSYEKQDPQSAGRKKLLSSTIIPPLAITVSEEQRRLLRSYHKHLEDLGLELLFPDASDSLILVGKVPLCFVEREASELRRGRSTVTKSIVEELIREQVELLQTTGGIQGTLPLTVQKVLASQACHGAIKFNDRLSLEESCRLIEALSLSQLPFQCAHGRPSMLPLADLDHLEQEKQVKPNLAKLRKMVRAWHLFGKTEQNLQQPIRPCEPP

Target ORF information:

RefSeq Version	XM_006515706.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mutL homolog 3 (Mlh3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515706.3

ORF Insert Sequence:

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGG GGCTCAGTAC AGGTGTCATC CATTTAGAAG CGATCTTGTT 
CTTCCTTTCC TGCCAAGAGC TCAGGAAGAG AGGACCATGC TGAGGCACAA CAGCAATGCT 
GTGGATGCTG CTGCTGCTGC TGTCAGTGAA CCCCTTCAGT CTCTGTTTTC AGAATGGAGC 
AATCCAGTGT TTGCTCGATA CCCAGAGGTT GCTGTTGATG TCAGCAGTGG CCAGGCTGAG 
AGCTTAGCCG TTAAAATTCA CAACGTCCTG TATCCCTATC GCTTCACCAA AGAGATGATT 
CACTCAGTGA AGGTTCTCCA GCAAGTGGAT AACAAGTTTA TTGCCTGCTT AATGAGCACG 
AGGATGGATG AGGATGGCCG AACAGGTGGA AACCTGTTAG TCCTGGTGGA CCAGCATGCT 
GCCCATGAAC GCATTCGTTT GGAGCAGCTT ATTACTGATT CCTATGAGAA ACAAGATCCA 
CAAAGCGCTG GCCGGAAGAA ATTATTGTCT TCCACAATAA TCCCTCCACT GGCAATCACC 
GTGTCAGAGG AACAAAGGAG ACTCTTACGG TCTTACCACA AACATTTAGA AGATCTGGGG 
CTTGAGTTGC TCTTTCCAGA TGCTAGTGAT TCTCTGATCC TGGTGGGAAA AGTGCCGCTC 
TGCTTTGTAG AGAGAGAAGC TAGTGAGCTT CGAAGAGGAC GCTCTACTGT GACTAAGAGT 
ATTGTGGAGG AATTAATTCG AGAACAAGTG GAGCTGCTCC AGACCACAGG AGGTATCCAA 
GGGACACTGC CACTGACTGT CCAGAAGGTG TTGGCCTCCC AGGCCTGCCA TGGGGCTATT 
AAGTTTAATG ATCGTCTGAG CCTAGAAGAG AGCTGCCGCC TCATCGAAGC TCTGTCCTTG 
TCCCAGCTGC CATTTCAGTG TGCTCATGGG AGACCCTCAA TGCTGCCCTT AGCTGACCTG 
GACCACTTGG AGCAGGAAAA ACAGGTTAAA CCCAACCTTG CTAAACTTCG CAAAATGGTT 
CGTGCCTGGC ATCTCTTTGG AAAAACAGAG CAGAACCTGC AGCAGCCTAT ACGTCCTTGT 
GAGCCTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlh3 ( XM_006515703.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515766.1 Mus musculus mutL homolog 3 (Mlh3), transcript variant X1, mRNA.

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CloneID	OMu47734
Clone ID Related Accession (Same CDS sequence)	XM_006515706.3 , XM_006515706.4 , XM_006515703.4 , XM_006515704.4 , XM_006515703.3 , XM_006515704.3
Accession Version	XM_006515703.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA mismatch repair protein Mlh3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGG GGCTCAGTAC AGGTGTCATC CATTTAGAAG CGATCTTGTT 
CTTCCTTTCC TGCCAAGAGC TCAGGAAGAG AGGACCATGC TGAGGCACAA CAGCAATGCT 
GTGGATGCTG CTGCTGCTGC TGTCAGTGAA CCCCTTCAGT CTCTGTTTTC AGAATGGAGC 
AATCCAGTGT TTGCTCGATA CCCAGAGGTT GCTGTTGATG TCAGCAGTGG CCAGGCTGAG 
AGCTTAGCCG TTAAAATTCA CAACGTCCTG TATCCCTATC GCTTCACCAA AGAGATGATT 
CACTCAGTGA AGGTTCTCCA GCAAGTGGAT AACAAGTTTA TTGCCTGCTT AATGAGCACG 
AGGATGGATG AGGATGGCCG AACAGGTGGA AACCTGTTAG TCCTGGTGGA CCAGCATGCT 
GCCCATGAAC GCATTCGTTT GGAGCAGCTT ATTACTGATT CCTATGAGAA ACAAGATCCA 
CAAAGCGCTG GCCGGAAGAA ATTATTGTCT TCCACAATAA TCCCTCCACT GGCAATCACC 
GTGTCAGAGG AACAAAGGAG ACTCTTACGG TCTTACCACA AACATTTAGA AGATCTGGGG 
CTTGAGTTGC TCTTTCCAGA TGCTAGTGAT TCTCTGATCC TGGTGGGAAA AGTGCCGCTC 
TGCTTTGTAG AGAGAGAAGC TAGTGAGCTT CGAAGAGGAC GCTCTACTGT GACTAAGAGT 
ATTGTGGAGG AATTAATTCG AGAACAAGTG GAGCTGCTCC AGACCACAGG AGGTATCCAA 
GGGACACTGC CACTGACTGT CCAGAAGGTG TTGGCCTCCC AGGCCTGCCA TGGGGCTATT 
AAGTTTAATG ATCGTCTGAG CCTAGAAGAG AGCTGCCGCC TCATCGAAGC TCTGTCCTTG 
TCCCAGCTGC CATTTCAGTG TGCTCATGGG AGACCCTCAA TGCTGCCCTT AGCTGACCTG 
GACCACTTGG AGCAGGAAAA ACAGGTTAAA CCCAACCTTG CTAAACTTCG CAAAATGGTT 
CGTGCCTGGC ATCTCTTTGG AAAAACAGAG CAGAACCTGC AGCAGCCTAT ACGTCCTTGT 
GAGCCTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515766.1
CDS	172..4503
Translation	MIRCLSDDVKTKLRSGLAISSLGQCVEELTLNSIDAEATCVAIRVNMETFQVQVIDNGLGMAGDDVEKVGNRYFTSKCHSVRDLENPAFYGFRGEALASIADMAGAVEISSKKNTTLKTFVKMFQNGKALATHEADLTRPSVGTTVTVYNLFYQFPVRRKSMDPRLEFEKVRQRVEALSLMHPSISFSLRNDVSGSMVLQLPKTKDICSRFCQIYGLGKSQKLREIRFKYKEFEFSGYISSEAHYNKNMQFLFVNRRLVLRTKLHKLIDFLLRKESIICRPKNGSASRQMNSSPRHRSASELHGIYVINVQCPFCEYDVCIEPAKTLIEFQSWDTVLICIQEGVKRFLKQEKLFVELSGEDIKEFNEDNGFSLFGTTLQTHVSTHEKCDQSSFREACNKILDSYEMFNLQSKAVKRIATLENKTRQNPGDSETIRKKTVGSLYTDASDGPCYSKSVESVLQDSNNSAYLEPRVSEEEVAKTSHSGENEKWKKSFLENKTSGRIHETSPKMFSSPIQMHHLLEEREADLEMQTISSTVNVMAANIPQNNDIPSQLEKWKDAPEVGCQPLPFETTLLRVRGTQRKKERRKKEPSSRGRVNVFSYGQVKLCSTGFITHVVQSEHAKSTETEHSFKNYARPGPVSAQETFGKRTHHAIETPDSSDLTSTLSKESSQPPNKRFCRTNTGYGTENKPVATDDNLALFQESCKESHTDRLLPDASSFPWCRYVSDGCRKIDKRGSFKQVVRRKLSLRSQVGSLEKFKRQYGKVSSSLDTEKDNNTEVRTHLDPQNEPDVLLKDKSHLDMSDGCEITTVEHSETCQPLSPILYPEKILFSKEDRLEQMPHLRESPITLEELSHCNRKADVEKSAASLASKLSKLKDSEKEMQTVGMTGHTSELPDSNPSWKDNSQCTRLDLDFCELLKNKLEKIESDMLPMADSATEDGPINKNSELHPNNTTDDTEKPETPLLFPCNDSKISRDSDVLIRTSEQPTGNPDSVGKVIMSQVEDGIGSQGGVCPQGDESKARSCSKNEPNAHCMDWQQHFDVTLGRMVYINRMTGLSTFVAPTDDLHTACTKDLTTVAVDVLLGNGAQYRCHPFRSDLVLPFLPRAQEERTMLRHNSNAVDAAAAAVSEPLQSLFSEWSNPVFARYPEVAVDVSSGQAESLAVKIHNVLYPYRFTKEMIHSVKVLQQVDNKFIACLMSTRMDEDGRTGGNLLVLVDQHAAHERIRLEQLITDSYEKQDPQSAGRKKLLSSTIIPPLAITVSEEQRRLLRSYHKHLEDLGLELLFPDASDSLILVGKVPLCFVEREASELRRGRSTVTKSIVEELIREQVELLQTTGGIQGTLPLTVQKVLASQACHGAIKFNDRLSLEESCRLIEALSLSQLPFQCAHGRPSMLPLADLDHLEQEKQVKPNLAKLRKMVRAWHLFGKTEQNLQQPIRPCEPP

Target ORF information:

RefSeq Version	XM_006515703.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mutL homolog 3 (Mlh3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515703.3

ORF Insert Sequence:

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGG GGCTCAGTAC AGGTGTCATC CATTTAGAAG CGATCTTGTT 
CTTCCTTTCC TGCCAAGAGC TCAGGAAGAG AGGACCATGC TGAGGCACAA CAGCAATGCT 
GTGGATGCTG CTGCTGCTGC TGTCAGTGAA CCCCTTCAGT CTCTGTTTTC AGAATGGAGC 
AATCCAGTGT TTGCTCGATA CCCAGAGGTT GCTGTTGATG TCAGCAGTGG CCAGGCTGAG 
AGCTTAGCCG TTAAAATTCA CAACGTCCTG TATCCCTATC GCTTCACCAA AGAGATGATT 
CACTCAGTGA AGGTTCTCCA GCAAGTGGAT AACAAGTTTA TTGCCTGCTT AATGAGCACG 
AGGATGGATG AGGATGGCCG AACAGGTGGA AACCTGTTAG TCCTGGTGGA CCAGCATGCT 
GCCCATGAAC GCATTCGTTT GGAGCAGCTT ATTACTGATT CCTATGAGAA ACAAGATCCA 
CAAAGCGCTG GCCGGAAGAA ATTATTGTCT TCCACAATAA TCCCTCCACT GGCAATCACC 
GTGTCAGAGG AACAAAGGAG ACTCTTACGG TCTTACCACA AACATTTAGA AGATCTGGGG 
CTTGAGTTGC TCTTTCCAGA TGCTAGTGAT TCTCTGATCC TGGTGGGAAA AGTGCCGCTC 
TGCTTTGTAG AGAGAGAAGC TAGTGAGCTT CGAAGAGGAC GCTCTACTGT GACTAAGAGT 
ATTGTGGAGG AATTAATTCG AGAACAAGTG GAGCTGCTCC AGACCACAGG AGGTATCCAA 
GGGACACTGC CACTGACTGT CCAGAAGGTG TTGGCCTCCC AGGCCTGCCA TGGGGCTATT 
AAGTTTAATG ATCGTCTGAG CCTAGAAGAG AGCTGCCGCC TCATCGAAGC TCTGTCCTTG 
TCCCAGCTGC CATTTCAGTG TGCTCATGGG AGACCCTCAA TGCTGCCCTT AGCTGACCTG 
GACCACTTGG AGCAGGAAAA ACAGGTTAAA CCCAACCTTG CTAAACTTCG CAAAATGGTT 
CGTGCCTGGC ATCTCTTTGG AAAAACAGAG CAGAACCTGC AGCAGCCTAT ACGTCCTTGT 
GAGCCTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlh3 ( XM_006515704.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515767.1 Mus musculus mutL homolog 3 (Mlh3), transcript variant X2, mRNA.

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CloneID	OMu47734
Clone ID Related Accession (Same CDS sequence)	XM_006515706.3 , XM_006515706.4 , XM_006515703.4 , XM_006515704.4 , XM_006515703.3 , XM_006515704.3
Accession Version	XM_006515704.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA mismatch repair protein Mlh3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGG GGCTCAGTAC AGGTGTCATC CATTTAGAAG CGATCTTGTT 
CTTCCTTTCC TGCCAAGAGC TCAGGAAGAG AGGACCATGC TGAGGCACAA CAGCAATGCT 
GTGGATGCTG CTGCTGCTGC TGTCAGTGAA CCCCTTCAGT CTCTGTTTTC AGAATGGAGC 
AATCCAGTGT TTGCTCGATA CCCAGAGGTT GCTGTTGATG TCAGCAGTGG CCAGGCTGAG 
AGCTTAGCCG TTAAAATTCA CAACGTCCTG TATCCCTATC GCTTCACCAA AGAGATGATT 
CACTCAGTGA AGGTTCTCCA GCAAGTGGAT AACAAGTTTA TTGCCTGCTT AATGAGCACG 
AGGATGGATG AGGATGGCCG AACAGGTGGA AACCTGTTAG TCCTGGTGGA CCAGCATGCT 
GCCCATGAAC GCATTCGTTT GGAGCAGCTT ATTACTGATT CCTATGAGAA ACAAGATCCA 
CAAAGCGCTG GCCGGAAGAA ATTATTGTCT TCCACAATAA TCCCTCCACT GGCAATCACC 
GTGTCAGAGG AACAAAGGAG ACTCTTACGG TCTTACCACA AACATTTAGA AGATCTGGGG 
CTTGAGTTGC TCTTTCCAGA TGCTAGTGAT TCTCTGATCC TGGTGGGAAA AGTGCCGCTC 
TGCTTTGTAG AGAGAGAAGC TAGTGAGCTT CGAAGAGGAC GCTCTACTGT GACTAAGAGT 
ATTGTGGAGG AATTAATTCG AGAACAAGTG GAGCTGCTCC AGACCACAGG AGGTATCCAA 
GGGACACTGC CACTGACTGT CCAGAAGGTG TTGGCCTCCC AGGCCTGCCA TGGGGCTATT 
AAGTTTAATG ATCGTCTGAG CCTAGAAGAG AGCTGCCGCC TCATCGAAGC TCTGTCCTTG 
TCCCAGCTGC CATTTCAGTG TGCTCATGGG AGACCCTCAA TGCTGCCCTT AGCTGACCTG 
GACCACTTGG AGCAGGAAAA ACAGGTTAAA CCCAACCTTG CTAAACTTCG CAAAATGGTT 
CGTGCCTGGC ATCTCTTTGG AAAAACAGAG CAGAACCTGC AGCAGCCTAT ACGTCCTTGT 
GAGCCTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515767.1
CDS	237..4568
Translation	MIRCLSDDVKTKLRSGLAISSLGQCVEELTLNSIDAEATCVAIRVNMETFQVQVIDNGLGMAGDDVEKVGNRYFTSKCHSVRDLENPAFYGFRGEALASIADMAGAVEISSKKNTTLKTFVKMFQNGKALATHEADLTRPSVGTTVTVYNLFYQFPVRRKSMDPRLEFEKVRQRVEALSLMHPSISFSLRNDVSGSMVLQLPKTKDICSRFCQIYGLGKSQKLREIRFKYKEFEFSGYISSEAHYNKNMQFLFVNRRLVLRTKLHKLIDFLLRKESIICRPKNGSASRQMNSSPRHRSASELHGIYVINVQCPFCEYDVCIEPAKTLIEFQSWDTVLICIQEGVKRFLKQEKLFVELSGEDIKEFNEDNGFSLFGTTLQTHVSTHEKCDQSSFREACNKILDSYEMFNLQSKAVKRIATLENKTRQNPGDSETIRKKTVGSLYTDASDGPCYSKSVESVLQDSNNSAYLEPRVSEEEVAKTSHSGENEKWKKSFLENKTSGRIHETSPKMFSSPIQMHHLLEEREADLEMQTISSTVNVMAANIPQNNDIPSQLEKWKDAPEVGCQPLPFETTLLRVRGTQRKKERRKKEPSSRGRVNVFSYGQVKLCSTGFITHVVQSEHAKSTETEHSFKNYARPGPVSAQETFGKRTHHAIETPDSSDLTSTLSKESSQPPNKRFCRTNTGYGTENKPVATDDNLALFQESCKESHTDRLLPDASSFPWCRYVSDGCRKIDKRGSFKQVVRRKLSLRSQVGSLEKFKRQYGKVSSSLDTEKDNNTEVRTHLDPQNEPDVLLKDKSHLDMSDGCEITTVEHSETCQPLSPILYPEKILFSKEDRLEQMPHLRESPITLEELSHCNRKADVEKSAASLASKLSKLKDSEKEMQTVGMTGHTSELPDSNPSWKDNSQCTRLDLDFCELLKNKLEKIESDMLPMADSATEDGPINKNSELHPNNTTDDTEKPETPLLFPCNDSKISRDSDVLIRTSEQPTGNPDSVGKVIMSQVEDGIGSQGGVCPQGDESKARSCSKNEPNAHCMDWQQHFDVTLGRMVYINRMTGLSTFVAPTDDLHTACTKDLTTVAVDVLLGNGAQYRCHPFRSDLVLPFLPRAQEERTMLRHNSNAVDAAAAAVSEPLQSLFSEWSNPVFARYPEVAVDVSSGQAESLAVKIHNVLYPYRFTKEMIHSVKVLQQVDNKFIACLMSTRMDEDGRTGGNLLVLVDQHAAHERIRLEQLITDSYEKQDPQSAGRKKLLSSTIIPPLAITVSEEQRRLLRSYHKHLEDLGLELLFPDASDSLILVGKVPLCFVEREASELRRGRSTVTKSIVEELIREQVELLQTTGGIQGTLPLTVQKVLASQACHGAIKFNDRLSLEESCRLIEALSLSQLPFQCAHGRPSMLPLADLDHLEQEKQVKPNLAKLRKMVRAWHLFGKTEQNLQQPIRPCEPP

Target ORF information:

RefSeq Version	XM_006515704.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mutL homolog 3 (Mlh3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515704.3

ORF Insert Sequence:

ATGATCAGGT GTCTATCAGA TGACGTAAAA ACCAAGTTGC GTTCCGGTTT AGCCATAAGC 
TCCTTGGGCC AGTGTGTTGA AGAACTTACC CTTAACAGTA TTGATGCTGA AGCAACATGT 
GTGGCCATCA GAGTGAATAT GGAAACCTTC CAAGTTCAAG TGATAGACAA TGGACTTGGG 
ATGGCGGGGG ACGATGTAGA GAAGGTGGGA AACCGGTATT TTACTAGTAA ATGCCACTCA 
GTGCGGGACT TGGAGAACCC AGCATTTTAT GGCTTCCGAG GAGAGGCCTT GGCAAGTATA 
GCCGACATGG CTGGTGCTGT GGAGATTTCA TCCAAGAAAA ACACAACACT GAAAACCTTT 
GTGAAAATGT TTCAGAATGG AAAAGCCCTT GCCACCCATG AGGCTGATTT GACCAGACCA 
AGTGTGGGGA CTACAGTAAC GGTCTATAAC CTGTTTTACC AGTTTCCTGT GCGGAGGAAA 
AGCATGGATC CTAGACTAGA GTTTGAGAAA GTTCGGCAGA GGGTAGAAGC TCTCTCACTT 
ATGCACCCCT CCATTTCTTT CTCTTTGAGG AACGATGTAT CTGGATCCAT GGTTCTTCAG 
CTCCCTAAAA CCAAAGACAT ATGCTCTCGA TTCTGTCAAA TTTACGGATT GGGCAAGTCC 
CAAAAGTTAA GAGAAATACG TTTTAAATAC AAGGAATTTG AGTTCAGTGG CTACATCAGC 
TCTGAAGCAC ACTACAATAA GAATATGCAG TTTTTGTTTG TGAACAGAAG ACTAGTTTTA 
AGAACAAAGT TGCATAAACT TATTGACTTT TTATTAAGAA AAGAAAGCAT TATATGCAGG 
CCAAAGAATG GCTCTGCCAG TAGGCAAATG AATTCAAGTC CTCGACACCG TTCTGCCTCA 
GAACTCCACG GGATATATGT AATCAATGTG CAGTGCCCTT TCTGTGAGTA TGATGTCTGC 
ATAGAGCCAG CCAAAACTCT GATTGAGTTT CAGAGCTGGG ATACCGTGTT GATTTGTATT 
CAGGAAGGAG TAAAAAGGTT TTTAAAGCAA GAAAAATTAT TTGTAGAATT ATCAGGTGAA 
GATATTAAGG AATTTAATGA AGATAATGGT TTTAGTTTGT TTGGCACGAC TCTTCAGACA 
CATGTGTCTA CTCATGAGAA GTGTGACCAG AGCAGTTTCC GGGAAGCGTG TAATAAAATT 
CTGGATTCCT ATGAAATGTT TAATTTGCAG TCAAAAGCTG TGAAAAGAAT AGCTACTCTA 
GAAAATAAAA CCAGACAAAA CCCTGGTGAT TCAGAAACTA TCAGAAAAAA GACAGTGGGC 
TCATTGTACA CAGATGCATC GGATGGCCCG TGCTATAGTA AATCGGTAGA GTCTGTTTTA 
CAGGACAGCA ACAACAGTGC TTACTTAGAA CCGAGGGTGT CAGAAGAAGA GGTAGCCAAA 
ACATCACACT CCGGAGAAAA TGAGAAATGG AAAAAATCTT TTTTGGAAAA TAAGACTTCA 
GGAAGGATAC ATGAAACCAG TCCAAAAATG TTTTCAAGCC CCATCCAAAT GCATCACCTC 
CTTGAGGAGA GAGAGGCAGA TCTGGAAATG CAGACAATAA GTAGTACTGT TAATGTCATG 
GCTGCCAACA TTCCCCAAAA TAATGACATT CCGAGTCAAC TGGAGAAATG GAAAGATGCT 
CCTGAAGTGG GGTGCCAACC TCTGCCTTTT GAGACAACCT TATTAAGGGT ACGGGGTACT 
CAGAGGAAGA AGGAAAGAAG GAAAAAGGAG CCCAGTAGTC GTGGAAGAGT AAATGTTTTT 
AGTTATGGAC AAGTTAAATT ATGCTCCACT GGCTTTATAA CTCATGTAGT ACAAAGTGAG 
CACGCTAAAT CAACTGAAAC AGAACATTCA TTTAAAAATT ATGCTCGACC TGGTCCTGTA 
AGTGCCCAAG AAACATTTGG AAAAAGAACA CACCATGCAA TTGAGACTCC AGACAGCAGT 
GATTTAACAA GCACTTTAAG TAAAGAATCC AGTCAACCGC CCAACAAAAG GTTTTGCAGA 
ACAAATACAG GTTACGGGAC AGAGAACAAA CCTGTAGCAA CAGATGACAA CTTGGCTCTT 
TTTCAGGAAA GCTGTAAAGA ATCACACACA GATCGCCTTT TGCCTGATGC ATCCTCCTTC 
CCATGGTGTA GATATGTTTC CGATGGTTGT AGGAAAATAG ATAAAAGGGG TTCCTTCAAA 
CAGGTAGTCC GTAGGAAGCT AAGCTTGCGT TCACAAGTAG GGTCTTTAGA GAAGTTTAAG 
AGGCAGTATG GGAAGGTCAG CAGTTCCCTA GATACAGAAA AGGATAATAA CACTGAAGTC 
AGGACTCATC TTGATCCTCA AAATGAACCC GATGTTCTTC TGAAAGACAA GAGCCACTTA 
GATATGTCTG ATGGTTGTGA GATCACTACT GTGGAGCACA GTGAGACTTG TCAACCATTA 
AGTCCCATCC TGTACCCAGA AAAAATTTTA TTTTCCAAAG AAGATCGCTT AGAACAGATG 
CCTCATTTGA GAGAAAGTCC TATAACTCTG GAAGAATTAT CTCACTGTAA CAGAAAAGCT 
GATGTTGAGA AGTCCGCTGC ATCACTGGCT TCTAAATTAT CCAAACTAAA GGATTCTGAA 
AAAGAGATGC AAACCGTGGG GATGACAGGT CATACTAGTG AACTTCCAGA TTCAAATCCC 
AGTTGGAAAG ATAATAGCCA GTGCACTAGG TTAGACTTGG ATTTTTGTGA ATTATTAAAA 
AACAAACTTG AAAAAATAGA GAGTGATATG CTTCCAATGG CAGATTCTGC CACAGAGGAT 
GGTCCCATCA ATAAAAACAG TGAACTACAT CCTAACAATA CAACGGATGA CACAGAGAAA 
CCAGAAACTC CTTTGCTGTT CCCCTGTAAT GATTCTAAAA TCAGCAGAGA TTCAGATGTT 
CTTATCAGAA CTTCAGAACA ACCTACAGGA AACCCTGACT CTGTCGGTAA AGTGATAATG 
AGTCAGGTAG AGGATGGCAT TGGCAGCCAA GGTGGAGTCT GTCCCCAGGG TGATGAATCT 
AAGGCAAGAT CTTGTTCCAA AAATGAACCA AACGCACACT GTATGGATTG GCAGCAGCAT 
TTTGATGTAA CCCTGGGAAG AATGGTTTAC ATCAACAGAA TGACAGGACT TAGCACATTC 
GTTGCTCCAA CTGACGACCT TCATACTGCT TGTACTAAAG ATCTGACAAC TGTGGCTGTG 
GATGTCCTGC TTGGGAATGG GGCTCAGTAC AGGTGTCATC CATTTAGAAG CGATCTTGTT 
CTTCCTTTCC TGCCAAGAGC TCAGGAAGAG AGGACCATGC TGAGGCACAA CAGCAATGCT 
GTGGATGCTG CTGCTGCTGC TGTCAGTGAA CCCCTTCAGT CTCTGTTTTC AGAATGGAGC 
AATCCAGTGT TTGCTCGATA CCCAGAGGTT GCTGTTGATG TCAGCAGTGG CCAGGCTGAG 
AGCTTAGCCG TTAAAATTCA CAACGTCCTG TATCCCTATC GCTTCACCAA AGAGATGATT 
CACTCAGTGA AGGTTCTCCA GCAAGTGGAT AACAAGTTTA TTGCCTGCTT AATGAGCACG 
AGGATGGATG AGGATGGCCG AACAGGTGGA AACCTGTTAG TCCTGGTGGA CCAGCATGCT 
GCCCATGAAC GCATTCGTTT GGAGCAGCTT ATTACTGATT CCTATGAGAA ACAAGATCCA 
CAAAGCGCTG GCCGGAAGAA ATTATTGTCT TCCACAATAA TCCCTCCACT GGCAATCACC 
GTGTCAGAGG AACAAAGGAG ACTCTTACGG TCTTACCACA AACATTTAGA AGATCTGGGG 
CTTGAGTTGC TCTTTCCAGA TGCTAGTGAT TCTCTGATCC TGGTGGGAAA AGTGCCGCTC 
TGCTTTGTAG AGAGAGAAGC TAGTGAGCTT CGAAGAGGAC GCTCTACTGT GACTAAGAGT 
ATTGTGGAGG AATTAATTCG AGAACAAGTG GAGCTGCTCC AGACCACAGG AGGTATCCAA 
GGGACACTGC CACTGACTGT CCAGAAGGTG TTGGCCTCCC AGGCCTGCCA TGGGGCTATT 
AAGTTTAATG ATCGTCTGAG CCTAGAAGAG AGCTGCCGCC TCATCGAAGC TCTGTCCTTG 
TCCCAGCTGC CATTTCAGTG TGCTCATGGG AGACCCTCAA TGCTGCCCTT AGCTGACCTG 
GACCACTTGG AGCAGGAAAA ACAGGTTAAA CCCAACCTTG CTAAACTTCG CAAAATGGTT 
CGTGCCTGGC ATCTCTTTGG AAAAACAGAG CAGAACCTGC AGCAGCCTAT ACGTCCTTGT 
GAGCCTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Age-Dependent Alterations in Meiotic Recombination Cause Chromosome Segregation Errors in Spermatocytes.
Cell171(3)601-614.e13(2017 Oct)
Zelazowski MJ,Sandoval M,Paniker L,Hamilton HM,Han J,Gribbell MA,Kang R,Cole F

Mammalian CNTD1 is critical for meiotic crossover maturation and deselection of excess precrossover sites.
The Journal of cell biology205(5)633-41(2014 Jun)
Holloway JK,Sun X,Yokoo R,Villeneuve AM,Cohen PE

Antagonistic roles of ubiquitin ligase HEI10 and SUMO ligase RNF212 regulate meiotic recombination.
Nature genetics46(2)194-9(2014 Feb)
Qiao H,Prasada Rao HB,Yang Y,Fong JH,Cloutier JM,Deacon DC,Nagel KE,Swartz RK,Strong E,Holloway JK,Cohen PE,Schimenti J,Ward J,Hunter N

Mismatch repair genes Mlh1 and Mlh3 modify CAG instability in Huntington's disease mice: genome-wide and candidate approaches.
PLoS genetics9(10)e1003930(2013 Oct)
Pinto RM,Dragileva E,Kirby A,Lloret A,Lopez E,St Claire J,Panigrahi GB,Hou C,Holloway K,Gillis T,Guide JR,Cohen PE,Li GM,Pearson CE,Daly MJ,Wheeler VC

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Mlh3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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