MBD5 cDNA ORF clone, Bos taurus(cattle)

The following MBD5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MBD5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227532 XM_024977299.1
Latest version!
Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa54962 XM_024977305.1
Latest version!
Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa16449 NM_001191268.1
Latest version!
Bos taurus methyl-CpG binding domain protein 5 (MBD5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa16448 XM_015475435.2
Latest version!
Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa16449 XM_015475445.2
Latest version!
Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54961 XM_015475432.2
Latest version!
Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa54961 XM_015475434.2
Latest version!
Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa54963 XM_015475436.2
Latest version!
Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OBa16449 XM_015475445.1 Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa16448 XM_015475435.1 Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa54961 XM_015475432.1 Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa54961 XM_015475434.1 Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa54963 XM_015475436.1 Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa227532
    Clone ID Related Accession (Same CDS sequence) XM_024977299.1
    Accession Version XM_024977299.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product methyl-CpG-binding domain protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAAACCC CACACCCTTC TCTGGTGCTC ACCAGTCCCG GAGGAGGAAC AAATGCAACT 
    CCAGTAGTAC CTTCACGGGC AGCAACTCCA AGATCTGTAA GAAATAAGTC ACATGAAGGA
    ATTACAAATT CTGTAATGCC TGAATGTAAG AATCCTTTCA AGTTAATGAT TGGATCATCA
    AACACCATGG GAAGACTATA TGTACAAGAA CTGCCTGGAA GCCAGCAACA GGAACCCCAC
    CCTGTCTACC CCCGGCAGAG ATTGGGCAGC AATGAGCATG GACAGAAGTC TCCATTCCGT
    GGCAGCCATG GAGGCCTGCC CAGCCCCGCG TCGTCAGGTT CCCAGATATA TGGAGACGGC
    TCGATCTCGC CAAGGACTGA CCCACTCGGA AGCCCTGATG TCTTCACAAG AAATAATCCT
    GGTTTTCACG GAGCTCCCAA TTCTAGTCCT ATTCACCTGA ATAGGACTCC TCTTTCTCCA
    CCTTCAGTAA TGCTACATGG TTCTCCTGTA CAGTCATCCT GTGCAATGGC TGGAAGGACT
    AATATACCTC TTTCCCCAAC CTTGACTACA AAGAGTCCAG TAATGAAAAA ACCAATGTGT
    AATTTTTCAA CTAATATGGA AATACCACGA GCAATGTTCC ACCACAAACC ACCCCAAGGC
    CCACCTCCCC CTCCACCTTC TTGTGCTCTT CAGAAAAAGC CATTAACATC TGAGAAAGAT
    CCACTTGGCA TTCTTGACCC TATTCCTAGT AAACCGGTGA ATCAGAACCC TGTTATCATT
    AATCCAACTA GTTTCCATTC AAATGTCCAC TCTCAGGTAC CTGTGATGAA TGTAAGCATG
    CCTCCTGCTG TTGTTCCTTT GCCAAGTAAT CTCCCTTTGC CAACCGTAAA ACCTGGTCAT
    ATGAATCATG GGAGTCATGT ACAAAGAGTT CAGCATTCAG CTTCAACCTC CCTGTCCCCT
    TCTCCAGTGA CATCCCCAGT GCACATGATG GGGACTGGAA TTGGAAGGAT TGAGGCATCG
    CCCCAAAGAT CACGCTCATC TTCCACATCA TCAGATCACG GAAATTTCAT GATGCCACCT
    CTAGGACCCC AGGCCAATTG CAGTGGTATT AAGGTTCCAC CCAGGTCACC AAGGTCAACA
    ATAGGGTCCC CAAGGCCATC AATGCCATCA AGCCCTTCTA CCAAGTCCGA TGGACATCAT
    CAGTACAAGG ATATCCCTAA CCCATTAATT GCTGGAATGA GTAATGTACT AAATCCCCCA
    AGCAGTGCAG CTTTTCCTAC TGCATCTGCC GGAAGTGGTT CTGTAAAGAG TCAGCCTGGT
    TTGCTGGGAA TGCCTTTAAA TCAGATCTTG AACCAGCACA ATGCTGCCTC CTTTCCAGCA
    AGTAGTTTAC TCTCAGCAGC AGCCAAAGCA CAGCTAGCAA ATCAAAACAA ACTTGCTGGT
    AACAACAGTA GCAGCAGTAG CAATTCTGGA GCTGTTGCTG GCAGTGGCAA CACTGAAGGA
    CATAGCACTT TAAACACCAT GTTCCCTCCT ACTGCCAACA TGCTTCTCCC GTCAGGTGAA
    GGGCAAAGTG GTCGAGCAGC ACTAAGAGAT AAGCTGATGT CTCAGCAAAA AGACTCATTG
    CGGAAAAGAA AACAGCCACC TACCACAGTG TTGAGTTTGC TCAGACAGTC TCAAATGGAT
    AGTTCTGCAG TTCCTAAACC TGGACCCGAC TTGCTAAGGA AGCAGGGTCA GGGGTCGTTT
    CCCATCAGTT CAATGTCTCA GTTACTACAG TCTATGAGTT GTCAAAGCTC TCACTTGAGT
    AGCAATAGTA CCCCGGGTTG TGGGGGCTCA AATACTGCTT TGCCTTGCTC TGCTAACCAG
    CTGCATTTTA CAGATCCCAG TATGAACTCC AGTGTTCTTC AGAATTCACT GACACAGAGC
    ATACCTTTAA GAGGGGAAGC CATGCACTGC CACAATGCAA ACACTAACTT TGTTCACAGT
    AATAGTCCAG TCCCAAACCA CCATCTTACA GGTTTAATAA ATCAGATTCA GGCTAGCGGG
    AACTGTGGGA TGCTCAGTCA GTCGGGCATG GCTTTAGGAA ATTCATTACA TCCCAATCCA
    CCTCAGTCAA GAATTTCAAC GTCCTCCACT CCAGTGATAC CAAACAGCAT TGTTAGCAGC
    TATAATCAAA CAAGTTCTGA AGCAGGCGGT TCAGGACCAT CCTCCTCCAT AGCCATAGCT
    GGCATCAACC ACCCTGCCAT CACAAAGACA ACATCTGTTC TTCAAGACGG CGTCATAGTC
    ACCACCGCAG CTGGAAACCC ACTGCAGAGT CAGCTGCCCA TTGGGAGTGA TTTTCCTTTT
    GTTGGCCAGG AGCACGCACT TCATTTTCCA TCCAACAGCA CTTCAAACAA CCATCTTCCA
    CACCCCTTGA ACCCCAGCCT CCTCAGTTCT CTACCTATCT CTTTGCCAGT GAATCAACAG
    CATCTCCTAA ACCAGAATCT ATTAAATATC TTCCAGCCTT CAGCAGGAGA AGGCAAGTCT
    GAGATCAACC TCCACCCTTT AGGTTTTCTC AACCCGAATG TAAACGCTGC TTTAGCTTTT
    CTCTCCGGTG ACATGGATGG GCAGGTATTG CAGCCTGTTC ACTTTCAACT CTTAGCAGCC
    CTGCTTCAGA ACCAAGCCCA CGCAGCTGCC ATGCTTCCCC TGCCATCTTT CAATCTGGCC
    ATCTCAGATA TTTTGCAACA GCAAAATACC CCCTTACCCT CATTAACACA GATGACAGCC
    CCACCAGACC ATTTGCCAAG CAATCAGTCA GAGAACAGCC GAGCTGAGAC CCTTTTAACC
    AACCCCCTGG GGAACCCTTT ACCAAGCTTT GCAGGCAGTG ACACTACTTT TAACCCCCTC
    TTCCTCCCAG CTGTCACTGG GGCCTCAGGA TTAATGGCCT TGAATCCCCA GCTGTTGGGA
    GGTGTCCTGA ACTCGGCATC GGCCAACACC GCTAATCATC CAGAGGTTTC CATAGCAACT
    TCTTCCCAGG CAACCACTAC CACAACCACT ACATCATCAG CAGTGGCAGC ACTGACTGTC
    TCAACACTTG GTGGGACAGC AGTGGTGTCA ATGGCAGAAA CATTGCTGAA TATATCTAAT
    AATGCTGGGA ATACACCTGG TCCAGCTAAA CTCAACAGTA ACTCTGTGGT GCCACAGCTA
    CTTAACCCTC TACTGGGGAC AGGTCTGCTT GGTGACATGC CCTCAATAAA CAGTACTTTG
    AATAACCATC AACTGACTCA TCTACAGTCG CTGTTAAACA ACAATCAGAT GTTTCCTCCA
    AATCAGCAGC AGCAGCAACT TCTTCAGGGG TACCAGAATC TCCAGGCATT TCAAGGACAG
    TCCACAATTC CTTGCCCAGC TAACAATAAC CCCATGGCTT GTCTGTTTCA GAATTTCCAG
    GTGAGAATGC AGGAAGATGC AGCTCTCCTA AACAAAAGAA TAAGCACTCA GCCGGGGCTC
    ACAGCACTTC CCGAAAATCC AAACACTACA CTTCCACCTT TCCAAGACAC CTCTTGTGAG
    TTGCAGTCGA GGATTGACCC ATCTCTAGGT CAGCAGGTGA AGGATGGCCT CATCATGGGG
    GGCCAAGGCG ATGCTTCCGT AGATGCTATT TACAAAGCAG TCGTGGATGC AGCCAGCAAA
    GGGATGCAGG TTGTCATCAC CACTGCTGTC AACAGTACAA CTCAGATCAG CCCCATTCCA
    GCTCTGAGTG CCATGAGTGC CTTCACTGCT TCCATCGGTG ACCCATTAAG TCTCCCCAGT
    GCTGTCAGTG CGGTCATTCA CGGACGAAAC ATGGGCAGTG TGGATCACGA TGGGCGGCTG
    AGGAGTGCGA GAGGGGCTCG GCTGCCTAGC AGTCTGGACC ACGGGAAGAA CTCCAGCGAA
    GGAGATGGCT TTGAATATTT CAAATCAGCA GGTTGCCACA CATCTAAAAA ACAGTGGGAT
    GGGGAGCAAA GCCCTGGAGG GGAGCGAAAC AGGTGGAAGT GTGAGGAATT TTTAGATCAT
    CCAGGCCACA TCCACAATAG TCCTTGTCAC GAAAGGCCCA ACAATGTCTC TACACTGCCA
    TTTCTGCCTG GGGAACAGCA CCCAATACTG TTACCACCGA GAAACTGTCA AGGGGATAAG
    ATTTTGGAGG AGAATTTCAG GTATAATAAC TACAAAAGAA CTATGATGAG TTTTAAGGAG
    AGACTAGAGA ACACTGTGGA AAGATGCGCA CACATTAACG GGAACAGATC TCGGCAGAGT
    CGGGGCTTTG GAGAGCTGCT GAGCACCACA AAGCAAGACT TTGTCCTAGA GGAGCAATCT
    CCGAGCTCCT CCAATAGTTT GGAGAGTTCT CTGGTCAAAG ACTACATCCA TTACAATGGA
    GACTTTAATG CCAAAAACAT TAACGGGTGT GTACCTAGCC CTTCAGACGC TAAAAGCATT
    AGTAGTGAAG ATGACCTAAG GAACCCAGAC TCCCCCTCTT CAAATGAATT GATCCCTTAT
    AGGCCAAGGA CGTTCAACGT TGGCGACTTG GTCTGGGGCC AAATCAAAGG ACTGACTTCC
    TGGCCTGGAA AATTAGTCAG AGAAGACGAC GTTCACAGTT CTTGTCAACA AAGCCCCGAG
    GAAGGGAAGG TCTGGGTAAT GTGGTCTGGT CTTCATGCCT TCACTCAGGT GGAGCCCGAG
    AAGTTGAAGA CACTAACGGA AGGTTTAGAA GCCTACAGCC GAGCCCGGAA AAGGAACAGA
    AAAAGTGGAA AGCTAAATAA CCATTTAGAA GCTGCTATTC ATGAGGCCAT GAGTGAACTG
    GACAAAATGT CTGGGACTGT TCACCAAATC CCCCAGGGAG ACAGACAGAT GAGACCTCCC
    AAACCCAAGA GGAGGAAGAT CTCCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833067.1
    CDS249..5138
    Translation

    Target ORF information:

    RefSeq Version XM_024977299.1
    Organism Bos taurus(cattle)
    Definition Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAAACCC CACACCCTTC TCTGGTGCTC ACCAGTCCCG GAGGAGGAAC AAATGCAACT 
    CCAGTAGTAC CTTCACGGGC AGCAACTCCA AGATCTGTAA GAAATAAGTC ACATGAAGGA
    ATTACAAATT CTGTAATGCC TGAATGTAAG AATCCTTTCA AGTTAATGAT TGGATCATCA
    AACACCATGG GAAGACTATA TGTACAAGAA CTGCCTGGAA GCCAGCAACA GGAACCCCAC
    CCTGTCTACC CCCGGCAGAG ATTGGGCAGC AATGAGCATG GACAGAAGTC TCCATTCCGT
    GGCAGCCATG GAGGCCTGCC CAGCCCCGCG TCGTCAGGTT CCCAGATATA TGGAGACGGC
    TCGATCTCGC CAAGGACTGA CCCACTCGGA AGCCCTGATG TCTTCACAAG AAATAATCCT
    GGTTTTCACG GAGCTCCCAA TTCTAGTCCT ATTCACCTGA ATAGGACTCC TCTTTCTCCA
    CCTTCAGTAA TGCTACATGG TTCTCCTGTA CAGTCATCCT GTGCAATGGC TGGAAGGACT
    AATATACCTC TTTCCCCAAC CTTGACTACA AAGAGTCCAG TAATGAAAAA ACCAATGTGT
    AATTTTTCAA CTAATATGGA AATACCACGA GCAATGTTCC ACCACAAACC ACCCCAAGGC
    CCACCTCCCC CTCCACCTTC TTGTGCTCTT CAGAAAAAGC CATTAACATC TGAGAAAGAT
    CCACTTGGCA TTCTTGACCC TATTCCTAGT AAACCGGTGA ATCAGAACCC TGTTATCATT
    AATCCAACTA GTTTCCATTC AAATGTCCAC TCTCAGGTAC CTGTGATGAA TGTAAGCATG
    CCTCCTGCTG TTGTTCCTTT GCCAAGTAAT CTCCCTTTGC CAACCGTAAA ACCTGGTCAT
    ATGAATCATG GGAGTCATGT ACAAAGAGTT CAGCATTCAG CTTCAACCTC CCTGTCCCCT
    TCTCCAGTGA CATCCCCAGT GCACATGATG GGGACTGGAA TTGGAAGGAT TGAGGCATCG
    CCCCAAAGAT CACGCTCATC TTCCACATCA TCAGATCACG GAAATTTCAT GATGCCACCT
    CTAGGACCCC AGGCCAATTG CAGTGGTATT AAGGTTCCAC CCAGGTCACC AAGGTCAACA
    ATAGGGTCCC CAAGGCCATC AATGCCATCA AGCCCTTCTA CCAAGTCCGA TGGACATCAT
    CAGTACAAGG ATATCCCTAA CCCATTAATT GCTGGAATGA GTAATGTACT AAATCCCCCA
    AGCAGTGCAG CTTTTCCTAC TGCATCTGCC GGAAGTGGTT CTGTAAAGAG TCAGCCTGGT
    TTGCTGGGAA TGCCTTTAAA TCAGATCTTG AACCAGCACA ATGCTGCCTC CTTTCCAGCA
    AGTAGTTTAC TCTCAGCAGC AGCCAAAGCA CAGCTAGCAA ATCAAAACAA ACTTGCTGGT
    AACAACAGTA GCAGCAGTAG CAATTCTGGA GCTGTTGCTG GCAGTGGCAA CACTGAAGGA
    CATAGCACTT TAAACACCAT GTTCCCTCCT ACTGCCAACA TGCTTCTCCC GTCAGGTGAA
    GGGCAAAGTG GTCGAGCAGC ACTAAGAGAT AAGCTGATGT CTCAGCAAAA AGACTCATTG
    CGGAAAAGAA AACAGCCACC TACCACAGTG TTGAGTTTGC TCAGACAGTC TCAAATGGAT
    AGTTCTGCAG TTCCTAAACC TGGACCCGAC TTGCTAAGGA AGCAGGGTCA GGGGTCGTTT
    CCCATCAGTT CAATGTCTCA GTTACTACAG TCTATGAGTT GTCAAAGCTC TCACTTGAGT
    AGCAATAGTA CCCCGGGTTG TGGGGGCTCA AATACTGCTT TGCCTTGCTC TGCTAACCAG
    CTGCATTTTA CAGATCCCAG TATGAACTCC AGTGTTCTTC AGAATTCACT GACACAGAGC
    ATACCTTTAA GAGGGGAAGC CATGCACTGC CACAATGCAA ACACTAACTT TGTTCACAGT
    AATAGTCCAG TCCCAAACCA CCATCTTACA GGTTTAATAA ATCAGATTCA GGCTAGCGGG
    AACTGTGGGA TGCTCAGTCA GTCGGGCATG GCTTTAGGAA ATTCATTACA TCCCAATCCA
    CCTCAGTCAA GAATTTCAAC GTCCTCCACT CCAGTGATAC CAAACAGCAT TGTTAGCAGC
    TATAATCAAA CAAGTTCTGA AGCAGGCGGT TCAGGACCAT CCTCCTCCAT AGCCATAGCT
    GGCATCAACC ACCCTGCCAT CACAAAGACA ACATCTGTTC TTCAAGACGG CGTCATAGTC
    ACCACCGCAG CTGGAAACCC ACTGCAGAGT CAGCTGCCCA TTGGGAGTGA TTTTCCTTTT
    GTTGGCCAGG AGCACGCACT TCATTTTCCA TCCAACAGCA CTTCAAACAA CCATCTTCCA
    CACCCCTTGA ACCCCAGCCT CCTCAGTTCT CTACCTATCT CTTTGCCAGT GAATCAACAG
    CATCTCCTAA ACCAGAATCT ATTAAATATC TTCCAGCCTT CAGCAGGAGA AGGCAAGTCT
    GAGATCAACC TCCACCCTTT AGGTTTTCTC AACCCGAATG TAAACGCTGC TTTAGCTTTT
    CTCTCCGGTG ACATGGATGG GCAGGTATTG CAGCCTGTTC ACTTTCAACT CTTAGCAGCC
    CTGCTTCAGA ACCAAGCCCA CGCAGCTGCC ATGCTTCCCC TGCCATCTTT CAATCTGGCC
    ATCTCAGATA TTTTGCAACA GCAAAATACC CCCTTACCCT CATTAACACA GATGACAGCC
    CCACCAGACC ATTTGCCAAG CAATCAGTCA GAGAACAGCC GAGCTGAGAC CCTTTTAACC
    AACCCCCTGG GGAACCCTTT ACCAAGCTTT GCAGGCAGTG ACACTACTTT TAACCCCCTC
    TTCCTCCCAG CTGTCACTGG GGCCTCAGGA TTAATGGCCT TGAATCCCCA GCTGTTGGGA
    GGTGTCCTGA ACTCGGCATC GGCCAACACC GCTAATCATC CAGAGGTTTC CATAGCAACT
    TCTTCCCAGG CAACCACTAC CACAACCACT ACATCATCAG CAGTGGCAGC ACTGACTGTC
    TCAACACTTG GTGGGACAGC AGTGGTGTCA ATGGCAGAAA CATTGCTGAA TATATCTAAT
    AATGCTGGGA ATACACCTGG TCCAGCTAAA CTCAACAGTA ACTCTGTGGT GCCACAGCTA
    CTTAACCCTC TACTGGGGAC AGGTCTGCTT GGTGACATGC CCTCAATAAA CAGTACTTTG
    AATAACCATC AACTGACTCA TCTACAGTCG CTGTTAAACA ACAATCAGAT GTTTCCTCCA
    AATCAGCAGC AGCAGCAACT TCTTCAGGGG TACCAGAATC TCCAGGCATT TCAAGGACAG
    TCCACAATTC CTTGCCCAGC TAACAATAAC CCCATGGCTT GTCTGTTTCA GAATTTCCAG
    GTGAGAATGC AGGAAGATGC AGCTCTCCTA AACAAAAGAA TAAGCACTCA GCCGGGGCTC
    ACAGCACTTC CCGAAAATCC AAACACTACA CTTCCACCTT TCCAAGACAC CTCTTGTGAG
    TTGCAGTCGA GGATTGACCC ATCTCTAGGT CAGCAGGTGA AGGATGGCCT CATCATGGGG
    GGCCAAGGCG ATGCTTCCGT AGATGCTATT TACAAAGCAG TCGTGGATGC AGCCAGCAAA
    GGGATGCAGG TTGTCATCAC CACTGCTGTC AACAGTACAA CTCAGATCAG CCCCATTCCA
    GCTCTGAGTG CCATGAGTGC CTTCACTGCT TCCATCGGTG ACCCATTAAG TCTCCCCAGT
    GCTGTCAGTG CGGTCATTCA CGGACGAAAC ATGGGCAGTG TGGATCACGA TGGGCGGCTG
    AGGAGTGCGA GAGGGGCTCG GCTGCCTAGC AGTCTGGACC ACGGGAAGAA CTCCAGCGAA
    GGAGATGGCT TTGAATATTT CAAATCAGCA GGTTGCCACA CATCTAAAAA ACAGTGGGAT
    GGGGAGCAAA GCCCTGGAGG GGAGCGAAAC AGGTGGAAGT GTGAGGAATT TTTAGATCAT
    CCAGGCCACA TCCACAATAG TCCTTGTCAC GAAAGGCCCA ACAATGTCTC TACACTGCCA
    TTTCTGCCTG GGGAACAGCA CCCAATACTG TTACCACCGA GAAACTGTCA AGGGGATAAG
    ATTTTGGAGG AGAATTTCAG GTATAATAAC TACAAAAGAA CTATGATGAG TTTTAAGGAG
    AGACTAGAGA ACACTGTGGA AAGATGCGCA CACATTAACG GGAACAGATC TCGGCAGAGT
    CGGGGCTTTG GAGAGCTGCT GAGCACCACA AAGCAAGACT TTGTCCTAGA GGAGCAATCT
    CCGAGCTCCT CCAATAGTTT GGAGAGTTCT CTGGTCAAAG ACTACATCCA TTACAATGGA
    GACTTTAATG CCAAAAACAT TAACGGGTGT GTACCTAGCC CTTCAGACGC TAAAAGCATT
    AGTAGTGAAG ATGACCTAAG GAACCCAGAC TCCCCCTCTT CAAATGAATT GATCCCTTAT
    AGGCCAAGGA CGTTCAACGT TGGCGACTTG GTCTGGGGCC AAATCAAAGG ACTGACTTCC
    TGGCCTGGAA AATTAGTCAG AGAAGACGAC GTTCACAGTT CTTGTCAACA AAGCCCCGAG
    GAAGGGAAGG TCTGGGTAAT GTGGTCTGGT CTTCATGCCT TCACTCAGGT GGAGCCCGAG
    AAGTTGAAGA CACTAACGGA AGGTTTAGAA GCCTACAGCC GAGCCCGGAA AAGGAACAGA
    AAAAGTGGAA AGCTAAATAA CCATTTAGAA GCTGCTATTC ATGAGGCCAT GAGTGAACTG
    GACAAAATGT CTGGGACTGT TCACCAAATC CCCCAGGGAG ACAGACAGAT GAGACCTCCC
    AAACCCAAGA GGAGGAAGAT CTCCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54962
    Clone ID Related Accession (Same CDS sequence) XM_024977305.1
    Accession Version XM_024977305.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product methyl-CpG-binding domain protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGCTG TCACTGGGGC CTCAGGATTA
    ATGGCCTTGA ATCCCCAGCT GTTGGGAGGT GTCCTGAACT CGGCATCGGC CAACACCGCT
    AATCATCCAG AGGTTTCCAT AGCAACTTCT TCCCAGGCAA CCACTACCAC AACCACTACA
    TCATCAGCAG TGGCAGCACT GACTGTCTCA ACACTTGGTG GGACAGCAGT GGTGTCAATG
    GCAGAAACAT TGCTGAATAT ATCTAATAAT GCTGGGAATA CACCTGGTCC AGCTAAACTC
    AACAGTAACT CTGTGGTGCC ACAGCTACTT AACCCTCTAC TGGGGACAGG TCTGCTTGGT
    GACATGCCCT CAATAAACAG TACTTTGAAT AACCATCAAC TGACTCATCT ACAGTCGCTG
    TTAAACAACA ATCAGATGTT TCCTCCAAAT CAGCAGCAGC AGCAACTTCT TCAGGGGTAC
    CAGAATCTCC AGGCATTTCA AGGACAGTCC ACAATTCCTT GCCCAGCTAA CAATAACCCC
    ATGGCTTGTC TGTTTCAGAA TTTCCAGGTG AGAATGCAGG AAGATGCAGC TCTCCTAAAC
    AAAAGAATAA GCACTCAGCC GGGGCTCACA GCACTTCCCG AAAATCCAAA CACTACACTT
    CCACCTTTCC AAGACACCTC TTGTGAGTTG CAGTCGAGGA TTGACCCATC TCTAGGTCAG
    CAGGTGAAGG ATGGCCTCAT CATGGGGGGC CAAGGCGATG CTTCCGTAGA TGCTATTTAC
    AAAGCAGTCG TGGATGCAGC CAGCAAAGGG ATGCAGGTTG TCATCACCAC TGCTGTCAAC
    AGTACAACTC AGATCAGCCC CATTCCAGCT CTGAGTGCCA TGAGTGCCTT CACTGCTTCC
    ATCGGTGACC CATTAAGTCT CCCCAGTGCT GTCAGTGCGG TCATTCACGG ACGAAACATG
    GGCAGTGTGG ATCACGATGG GCGGCTGAGG AGTGCGAGAG GGGCTCGGCT GCCTAGCAGT
    CTGGACCACG GGAAGAACTC CAGCGAAGGA GATGGCTTTG AATATTTCAA ATCAGCAGGT
    TGCCACACAT CTAAAAAACA GTGGGATGGG GAGCAAAGCC CTGGAGGGGA GCGAAACAGG
    TGGAAGTGTG AGGAATTTTT AGATCATCCA GGCCACATCC ACAATAGTCC TTGTCACGAA
    AGGCCCAACA ATGTCTCTAC ACTGCCATTT CTGCCTGGGG AACAGCACCC AATACTGTTA
    CCACCGAGAA ACTGTCAAGG GGATAAGATT TTGGAGGAGA ATTTCAGGTA TAATAACTAC
    AAAAGAACTA TGATGAGTTT TAAGGAGAGA CTAGAGAACA CTGTGGAAAG ATGCGCACAC
    ATTAACGGGA ACAGATCTCG GCAGAGTCGG GGCTTTGGAG AGCTGCTGAG CACCACAAAG
    CAAGACTTTG TCCTAGAGGA GCAATCTCCG AGCTCCTCCA ATAGTTTGGA GAGTTCTCTG
    GTCAAAGACT ACATCCATTA CAATGGAGAC TTTAATGCCA AAAACATTAA CGGGTGTGTA
    CCTAGCCCTT CAGACGCTAA AAGCATTAGT AGTGAAGATG ACCTAAGGAA CCCAGACTCC
    CCCTCTTCAA ATGAATTGAT CCCTTATAGG CCAAGGACGT TCAACGTTGG CGACTTGGTC
    TGGGGCCAAA TCAAAGGACT GACTTCCTGG CCTGGAAAAT TAGTCAGAGA AGACGACGTT
    CACAGTTCTT GTCAACAAAG CCCCGAGGAA GGGAAGGTCT GGGTAATGTG GTCTGGTCTT
    CATGCCTTCA CTCAGGTGGA GCCCGAGAAG TTGAAGACAC TAACGGAAGG TTTAGAAGCC
    TACAGCCGAG CCCGGAAAAG GAACAGAAAA AGTGGAAAGC TAAATAACCA TTTAGAAGCT
    GCTATTCATG AGGCCATGAG TGAACTGGAC AAAATGTCTG GGACTGTTCA CCAAATCCCC
    CAGGGAGACA GACAGATGAG ACCTCCCAAA CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833073.1
    CDS859..5715
    Translation

    Target ORF information:

    RefSeq Version XM_024977305.1
    Organism Bos taurus(cattle)
    Definition Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977305.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGCTG TCACTGGGGC CTCAGGATTA
    ATGGCCTTGA ATCCCCAGCT GTTGGGAGGT GTCCTGAACT CGGCATCGGC CAACACCGCT
    AATCATCCAG AGGTTTCCAT AGCAACTTCT TCCCAGGCAA CCACTACCAC AACCACTACA
    TCATCAGCAG TGGCAGCACT GACTGTCTCA ACACTTGGTG GGACAGCAGT GGTGTCAATG
    GCAGAAACAT TGCTGAATAT ATCTAATAAT GCTGGGAATA CACCTGGTCC AGCTAAACTC
    AACAGTAACT CTGTGGTGCC ACAGCTACTT AACCCTCTAC TGGGGACAGG TCTGCTTGGT
    GACATGCCCT CAATAAACAG TACTTTGAAT AACCATCAAC TGACTCATCT ACAGTCGCTG
    TTAAACAACA ATCAGATGTT TCCTCCAAAT CAGCAGCAGC AGCAACTTCT TCAGGGGTAC
    CAGAATCTCC AGGCATTTCA AGGACAGTCC ACAATTCCTT GCCCAGCTAA CAATAACCCC
    ATGGCTTGTC TGTTTCAGAA TTTCCAGGTG AGAATGCAGG AAGATGCAGC TCTCCTAAAC
    AAAAGAATAA GCACTCAGCC GGGGCTCACA GCACTTCCCG AAAATCCAAA CACTACACTT
    CCACCTTTCC AAGACACCTC TTGTGAGTTG CAGTCGAGGA TTGACCCATC TCTAGGTCAG
    CAGGTGAAGG ATGGCCTCAT CATGGGGGGC CAAGGCGATG CTTCCGTAGA TGCTATTTAC
    AAAGCAGTCG TGGATGCAGC CAGCAAAGGG ATGCAGGTTG TCATCACCAC TGCTGTCAAC
    AGTACAACTC AGATCAGCCC CATTCCAGCT CTGAGTGCCA TGAGTGCCTT CACTGCTTCC
    ATCGGTGACC CATTAAGTCT CCCCAGTGCT GTCAGTGCGG TCATTCACGG ACGAAACATG
    GGCAGTGTGG ATCACGATGG GCGGCTGAGG AGTGCGAGAG GGGCTCGGCT GCCTAGCAGT
    CTGGACCACG GGAAGAACTC CAGCGAAGGA GATGGCTTTG AATATTTCAA ATCAGCAGGT
    TGCCACACAT CTAAAAAACA GTGGGATGGG GAGCAAAGCC CTGGAGGGGA GCGAAACAGG
    TGGAAGTGTG AGGAATTTTT AGATCATCCA GGCCACATCC ACAATAGTCC TTGTCACGAA
    AGGCCCAACA ATGTCTCTAC ACTGCCATTT CTGCCTGGGG AACAGCACCC AATACTGTTA
    CCACCGAGAA ACTGTCAAGG GGATAAGATT TTGGAGGAGA ATTTCAGGTA TAATAACTAC
    AAAAGAACTA TGATGAGTTT TAAGGAGAGA CTAGAGAACA CTGTGGAAAG ATGCGCACAC
    ATTAACGGGA ACAGATCTCG GCAGAGTCGG GGCTTTGGAG AGCTGCTGAG CACCACAAAG
    CAAGACTTTG TCCTAGAGGA GCAATCTCCG AGCTCCTCCA ATAGTTTGGA GAGTTCTCTG
    GTCAAAGACT ACATCCATTA CAATGGAGAC TTTAATGCCA AAAACATTAA CGGGTGTGTA
    CCTAGCCCTT CAGACGCTAA AAGCATTAGT AGTGAAGATG ACCTAAGGAA CCCAGACTCC
    CCCTCTTCAA ATGAATTGAT CCCTTATAGG CCAAGGACGT TCAACGTTGG CGACTTGGTC
    TGGGGCCAAA TCAAAGGACT GACTTCCTGG CCTGGAAAAT TAGTCAGAGA AGACGACGTT
    CACAGTTCTT GTCAACAAAG CCCCGAGGAA GGGAAGGTCT GGGTAATGTG GTCTGGTCTT
    CATGCCTTCA CTCAGGTGGA GCCCGAGAAG TTGAAGACAC TAACGGAAGG TTTAGAAGCC
    TACAGCCGAG CCCGGAAAAG GAACAGAAAA AGTGGAAAGC TAAATAACCA TTTAGAAGCT
    GCTATTCATG AGGCCATGAG TGAACTGGAC AAAATGTCTG GGACTGTTCA CCAAATCCCC
    CAGGGAGACA GACAGATGAG ACCTCCCAAA CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16449
    Clone ID Related Accession (Same CDS sequence) XM_015475445.1 , XM_015475445.2 , NM_001191268.1
    Accession Version NM_001191268.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product methyl-CpG-binding domain protein 5
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000002.1. On Jul 17, 2010 this sequence version replaced XM_001787389.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGTG ACATGCCCTC AATAAACAGT
    ACTTTGAATA ACCATCAACT GACTCATCTA CAGTCGCTGT TAAACAACAA TCAGATGTTT
    CCTCCAAATC AGCAGCAGCA GCAACTTCTT CAGGGGTACC AGAATCTCCA GGCATTTCAA
    GGACAGTCCA CAATTCCTTG CCCAGCTAAC AATAACCCCA TGGCTTGTCT GTTTCAGAAT
    TTCCAGGTGA GAATGCAGGA AGATGCAGCT CTCCTAAACA AAAGAATAAG CACTCAGCCG
    GGGCTCACAG CACTTCCCGA AAATCCAAAC ACTACACTTC CACCTTTCCA AGACACCTCT
    TGTGAGTTGC AGTCGAGGAT TGACCCATCT CTAGGTCAGC AGGTGAAGGA TGGCCTCATC
    ATGGGGGGCC AAGGCGATGC TTCCGTAGAT GCTATTTACA AAGCAGTCGT GGATGCAGCC
    AGCAAAGGGA TGCAGGTTGT CATCACCACT GCTGTCAACA GTACAACTCA GATCAGCCCC
    ATTCCAGCTC TGAGTGCCAT GAGTGCCTTC ACTGCTTCCA TCGGTGACCC ATTAAGTCTC
    CCCAGTGCTG TCAGTGCGGT CATTCACGGA CGAAACATGG GCAGTGTGGA TCACGATGGG
    CGGCTGAGGA GTGCGAGAGG GGCTCGGCTG CCTAGCAGTC TGGACCACGG GAAGAACTCC
    AGCGAAGGAG ATGGCTTTGA ATATTTCAAA TCAGCAGGTT GCCACACATC TAAAAAACAG
    TGGGATGGGG AGCAAAGCCC TGGAGGGGAG CGAAACAGGT GGAAGTGTGA GGAATTTTTA
    GATCATCCAG GCCACATCCA CAATAGTCCT TGTCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TGCCTGGGGA ACAGCACCCA ATACTGTTAC CACCGAGAAA CTGTCAAGGG
    GATAAGATTT TGGAGGAGAA TTTCAGGTAT AATAACTACA AAAGAACTAT GATGAGTTTT
    AAGGAGAGAC TAGAGAACAC TGTGGAAAGA TGCGCACACA TTAACGGGAA CAGATCTCGG
    CAGAGTCGGG GCTTTGGAGA GCTGCTGAGC ACCACAAAGC AAGACTTTGT CCTAGAGGAG
    CAATCTCCGA GCTCCTCCAA TAGTTTGGAG AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AAACATTAAC GGGTGTGTAC CTAGCCCTTC AGACGCTAAA
    AGCATTAGTA GTGAAGATGA CCTAAGGAAC CCAGACTCCC CCTCTTCAAA TGAATTGATC
    CCTTATAGGC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGAAAATT AGTCAGAGAA GACGACGTTC ACAGTTCTTG TCAACAAAGC
    CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG TTGAAGACAC TAACGGAAGG TTTAGAAGCC
    TACAGCCGAG CCCGGAAAAG GAACAGAAAA AGTGGAAAGC TAAATAACCA TTTAGAAGCT
    GCTATTCATG AGGCCATGAG TGAACTGGAC AAAATGTCTG GGACTGTTCA CCAAATCCCC
    CAGGGAGACA GACAGATGAG ACCTCCCAAA CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178197.1
    CDS288..4784
    Translation

    Target ORF information:

    RefSeq Version NM_001191268.1
    Organism Bos taurus(cattle)
    Definition Bos taurus methyl-CpG binding domain protein 5 (MBD5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191268.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGTG ACATGCCCTC AATAAACAGT
    ACTTTGAATA ACCATCAACT GACTCATCTA CAGTCGCTGT TAAACAACAA TCAGATGTTT
    CCTCCAAATC AGCAGCAGCA GCAACTTCTT CAGGGGTACC AGAATCTCCA GGCATTTCAA
    GGACAGTCCA CAATTCCTTG CCCAGCTAAC AATAACCCCA TGGCTTGTCT GTTTCAGAAT
    TTCCAGGTGA GAATGCAGGA AGATGCAGCT CTCCTAAACA AAAGAATAAG CACTCAGCCG
    GGGCTCACAG CACTTCCCGA AAATCCAAAC ACTACACTTC CACCTTTCCA AGACACCTCT
    TGTGAGTTGC AGTCGAGGAT TGACCCATCT CTAGGTCAGC AGGTGAAGGA TGGCCTCATC
    ATGGGGGGCC AAGGCGATGC TTCCGTAGAT GCTATTTACA AAGCAGTCGT GGATGCAGCC
    AGCAAAGGGA TGCAGGTTGT CATCACCACT GCTGTCAACA GTACAACTCA GATCAGCCCC
    ATTCCAGCTC TGAGTGCCAT GAGTGCCTTC ACTGCTTCCA TCGGTGACCC ATTAAGTCTC
    CCCAGTGCTG TCAGTGCGGT CATTCACGGA CGAAACATGG GCAGTGTGGA TCACGATGGG
    CGGCTGAGGA GTGCGAGAGG GGCTCGGCTG CCTAGCAGTC TGGACCACGG GAAGAACTCC
    AGCGAAGGAG ATGGCTTTGA ATATTTCAAA TCAGCAGGTT GCCACACATC TAAAAAACAG
    TGGGATGGGG AGCAAAGCCC TGGAGGGGAG CGAAACAGGT GGAAGTGTGA GGAATTTTTA
    GATCATCCAG GCCACATCCA CAATAGTCCT TGTCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TGCCTGGGGA ACAGCACCCA ATACTGTTAC CACCGAGAAA CTGTCAAGGG
    GATAAGATTT TGGAGGAGAA TTTCAGGTAT AATAACTACA AAAGAACTAT GATGAGTTTT
    AAGGAGAGAC TAGAGAACAC TGTGGAAAGA TGCGCACACA TTAACGGGAA CAGATCTCGG
    CAGAGTCGGG GCTTTGGAGA GCTGCTGAGC ACCACAAAGC AAGACTTTGT CCTAGAGGAG
    CAATCTCCGA GCTCCTCCAA TAGTTTGGAG AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AAACATTAAC GGGTGTGTAC CTAGCCCTTC AGACGCTAAA
    AGCATTAGTA GTGAAGATGA CCTAAGGAAC CCAGACTCCC CCTCTTCAAA TGAATTGATC
    CCTTATAGGC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGAAAATT AGTCAGAGAA GACGACGTTC ACAGTTCTTG TCAACAAAGC
    CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG TTGAAGACAC TAACGGAAGG TTTAGAAGCC
    TACAGCCGAG CCCGGAAAAG GAACAGAAAA AGTGGAAAGC TAAATAACCA TTTAGAAGCT
    GCTATTCATG AGGCCATGAG TGAACTGGAC AAAATGTCTG GGACTGTTCA CCAAATCCCC
    CAGGGAGACA GACAGATGAG ACCTCCCAAA CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16448
    Clone ID Related Accession (Same CDS sequence) XM_015475435.1 , XM_015475435.2
    Accession Version XM_015475435.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product methyl-CpG-binding domain protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGCA AGTCTGAGAT CAACCTCCAC
    CCTTTAGGTT TTCTCAACCC GAATGTAAAC GCTGCTTTAG CTTTTCTCTC CGGTGACATG
    GATGGGCAGG TATTGCAGCC TGTTCACTTT CAACTCTTAG CAGCCCTGCT TCAGAACCAA
    GCCCACGCAG CTGCCATGCT TCCCCTGCCA TCTTTCAATC TGGCCATCTC AGATATTTTG
    CAACAGCAAA ATACCCCCTT ACCCTCATTA ACACAGATGA CAGCCCCACC AGACCATTTG
    CCAAGCAATC AGTCAGAGAA CAGCCGAGCT GAGACCCTTT TAACCAACCC CCTGGGGAAC
    CCTTTACCAA GCTTTGCAGG CAGTGACACT ACTTTTAACC CCCTCTTCCT CCCAGCTGTC
    ACTGGGGCCT CAGGATTAAT GGCCTTGAAT CCCCAGCTGT TGGGAGGTGT CCTGAACTCG
    GCATCGGCCA ACACCGCTAA TCATCCAGAG GTTTCCATAG CAACTTCTTC CCAGGCAACC
    ACTACCACAA CCACTACATC ATCAGCAGTG GCAGCACTGA CTGTCTCAAC ACTTGGTGGG
    ACAGCAGTGG TGTCAATGGC AGAAACATTG CTGAATATAT CTAATAATGC TGGGAATACA
    CCTGGTCCAG CTAAACTCAA CAGTAACTCT GTGGTGCCAC AGCTACTTAA CCCTCTACTG
    GGGACAGGTC TGCTTGGTGA CATGCCCTCA ATAAACAGTA CTTTGAATAA CCATCAACTG
    ACTCATCTAC AGTCGCTGTT AAACAACAAT CAGATGTTTC CTCCAAATCA GCAGCAGCAG
    CAACTTCTTC AGGGGTACCA GAATCTCCAG GCATTTCAAG GACAGTCCAC AATTCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAATT TCCAGGTGAG AATGCAGGAA
    GATGCAGCTC TCCTAAACAA AAGAATAAGC ACTCAGCCGG GGCTCACAGC ACTTCCCGAA
    AATCCAAACA CTACACTTCC ACCTTTCCAA GACACCTCTT GTGAGTTGCA GTCGAGGATT
    GACCCATCTC TAGGTCAGCA GGTGAAGGAT GGCCTCATCA TGGGGGGCCA AGGCGATGCT
    TCCGTAGATG CTATTTACAA AGCAGTCGTG GATGCAGCCA GCAAAGGGAT GCAGGTTGTC
    ATCACCACTG CTGTCAACAG TACAACTCAG ATCAGCCCCA TTCCAGCTCT GAGTGCCATG
    AGTGCCTTCA CTGCTTCCAT CGGTGACCCA TTAAGTCTCC CCAGTGCTGT CAGTGCGGTC
    ATTCACGGAC GAAACATGGG CAGTGTGGAT CACGATGGGC GGCTGAGGAG TGCGAGAGGG
    GCTCGGCTGC CTAGCAGTCT GGACCACGGG AAGAACTCCA GCGAAGGAGA TGGCTTTGAA
    TATTTCAAAT CAGCAGGTTG CCACACATCT AAAAAACAGT GGGATGGGGA GCAAAGCCCT
    GGAGGGGAGC GAAACAGGTG GAAGTGTGAG GAATTTTTAG ATCATCCAGG CCACATCCAC
    AATAGTCCTT GTCACGAAAG GCCCAACAAT GTCTCTACAC TGCCATTTCT GCCTGGGGAA
    CAGCACCCAA TACTGTTACC ACCGAGAAAC TGTCAAGGGG ATAAGATTTT GGAGGAGAAT
    TTCAGGTATA ATAACTACAA AAGAACTATG ATGAGTTTTA AGGAGAGACT AGAGAACACT
    GTGGAAAGAT GCGCACACAT TAACGGGAAC AGATCTCGGC AGAGTCGGGG CTTTGGAGAG
    CTGCTGAGCA CCACAAAGCA AGACTTTGTC CTAGAGGAGC AATCTCCGAG CTCCTCCAAT
    AGTTTGGAGA GTTCTCTGGT CAAAGACTAC ATCCATTACA ATGGAGACTT TAATGCCAAA
    AACATTAACG GGTGTGTACC TAGCCCTTCA GACGCTAAAA GCATTAGTAG TGAAGATGAC
    CTAAGGAACC CAGACTCCCC CTCTTCAAAT GAATTGATCC CTTATAGGCC AAGGACGTTC
    AACGTTGGCG ACTTGGTCTG GGGCCAAATC AAAGGACTGA CTTCCTGGCC TGGAAAATTA
    GTCAGAGAAG ACGACGTTCA CAGTTCTTGT CAACAAAGCC CCGAGGAAGG GAAGGTGGAG
    CCCGAGAAGT TGAAGACACT AACGGAAGGT TTAGAAGCCT ACAGCCGAGC CCGGAAAAGG
    AACAGAAAAA GTGGAAAGCT AAATAACCAT TTAGAAGCTG CTATTCATGA GGCCATGAGT
    GAACTGGACA AAATGTCTGG GACTGTTCAC CAAATCCCCC AGGGAGACAG ACAGATGAGA
    CCTCCCAAAC CCAAGAGGAG GAAGATCTCC AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330921.1
    CDS857..6052
    Translation

    Target ORF information:

    RefSeq Version XM_015475435.1
    Organism Bos taurus(cattle)
    Definition Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475435.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGCA AGTCTGAGAT CAACCTCCAC
    CCTTTAGGTT TTCTCAACCC GAATGTAAAC GCTGCTTTAG CTTTTCTCTC CGGTGACATG
    GATGGGCAGG TATTGCAGCC TGTTCACTTT CAACTCTTAG CAGCCCTGCT TCAGAACCAA
    GCCCACGCAG CTGCCATGCT TCCCCTGCCA TCTTTCAATC TGGCCATCTC AGATATTTTG
    CAACAGCAAA ATACCCCCTT ACCCTCATTA ACACAGATGA CAGCCCCACC AGACCATTTG
    CCAAGCAATC AGTCAGAGAA CAGCCGAGCT GAGACCCTTT TAACCAACCC CCTGGGGAAC
    CCTTTACCAA GCTTTGCAGG CAGTGACACT ACTTTTAACC CCCTCTTCCT CCCAGCTGTC
    ACTGGGGCCT CAGGATTAAT GGCCTTGAAT CCCCAGCTGT TGGGAGGTGT CCTGAACTCG
    GCATCGGCCA ACACCGCTAA TCATCCAGAG GTTTCCATAG CAACTTCTTC CCAGGCAACC
    ACTACCACAA CCACTACATC ATCAGCAGTG GCAGCACTGA CTGTCTCAAC ACTTGGTGGG
    ACAGCAGTGG TGTCAATGGC AGAAACATTG CTGAATATAT CTAATAATGC TGGGAATACA
    CCTGGTCCAG CTAAACTCAA CAGTAACTCT GTGGTGCCAC AGCTACTTAA CCCTCTACTG
    GGGACAGGTC TGCTTGGTGA CATGCCCTCA ATAAACAGTA CTTTGAATAA CCATCAACTG
    ACTCATCTAC AGTCGCTGTT AAACAACAAT CAGATGTTTC CTCCAAATCA GCAGCAGCAG
    CAACTTCTTC AGGGGTACCA GAATCTCCAG GCATTTCAAG GACAGTCCAC AATTCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAATT TCCAGGTGAG AATGCAGGAA
    GATGCAGCTC TCCTAAACAA AAGAATAAGC ACTCAGCCGG GGCTCACAGC ACTTCCCGAA
    AATCCAAACA CTACACTTCC ACCTTTCCAA GACACCTCTT GTGAGTTGCA GTCGAGGATT
    GACCCATCTC TAGGTCAGCA GGTGAAGGAT GGCCTCATCA TGGGGGGCCA AGGCGATGCT
    TCCGTAGATG CTATTTACAA AGCAGTCGTG GATGCAGCCA GCAAAGGGAT GCAGGTTGTC
    ATCACCACTG CTGTCAACAG TACAACTCAG ATCAGCCCCA TTCCAGCTCT GAGTGCCATG
    AGTGCCTTCA CTGCTTCCAT CGGTGACCCA TTAAGTCTCC CCAGTGCTGT CAGTGCGGTC
    ATTCACGGAC GAAACATGGG CAGTGTGGAT CACGATGGGC GGCTGAGGAG TGCGAGAGGG
    GCTCGGCTGC CTAGCAGTCT GGACCACGGG AAGAACTCCA GCGAAGGAGA TGGCTTTGAA
    TATTTCAAAT CAGCAGGTTG CCACACATCT AAAAAACAGT GGGATGGGGA GCAAAGCCCT
    GGAGGGGAGC GAAACAGGTG GAAGTGTGAG GAATTTTTAG ATCATCCAGG CCACATCCAC
    AATAGTCCTT GTCACGAAAG GCCCAACAAT GTCTCTACAC TGCCATTTCT GCCTGGGGAA
    CAGCACCCAA TACTGTTACC ACCGAGAAAC TGTCAAGGGG ATAAGATTTT GGAGGAGAAT
    TTCAGGTATA ATAACTACAA AAGAACTATG ATGAGTTTTA AGGAGAGACT AGAGAACACT
    GTGGAAAGAT GCGCACACAT TAACGGGAAC AGATCTCGGC AGAGTCGGGG CTTTGGAGAG
    CTGCTGAGCA CCACAAAGCA AGACTTTGTC CTAGAGGAGC AATCTCCGAG CTCCTCCAAT
    AGTTTGGAGA GTTCTCTGGT CAAAGACTAC ATCCATTACA ATGGAGACTT TAATGCCAAA
    AACATTAACG GGTGTGTACC TAGCCCTTCA GACGCTAAAA GCATTAGTAG TGAAGATGAC
    CTAAGGAACC CAGACTCCCC CTCTTCAAAT GAATTGATCC CTTATAGGCC AAGGACGTTC
    AACGTTGGCG ACTTGGTCTG GGGCCAAATC AAAGGACTGA CTTCCTGGCC TGGAAAATTA
    GTCAGAGAAG ACGACGTTCA CAGTTCTTGT CAACAAAGCC CCGAGGAAGG GAAGGTGGAG
    CCCGAGAAGT TGAAGACACT AACGGAAGGT TTAGAAGCCT ACAGCCGAGC CCGGAAAAGG
    AACAGAAAAA GTGGAAAGCT AAATAACCAT TTAGAAGCTG CTATTCATGA GGCCATGAGT
    GAACTGGACA AAATGTCTGG GACTGTTCAC CAAATCCCCC AGGGAGACAG ACAGATGAGA
    CCTCCCAAAC CCAAGAGGAG GAAGATCTCC AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16448
    Clone ID Related Accession (Same CDS sequence) XM_015475435.1 , XM_015475435.2
    Accession Version XM_015475435.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product methyl-CpG-binding domain protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475435.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGCA AGTCTGAGAT CAACCTCCAC
    CCTTTAGGTT TTCTCAACCC GAATGTAAAC GCTGCTTTAG CTTTTCTCTC CGGTGACATG
    GATGGGCAGG TATTGCAGCC TGTTCACTTT CAACTCTTAG CAGCCCTGCT TCAGAACCAA
    GCCCACGCAG CTGCCATGCT TCCCCTGCCA TCTTTCAATC TGGCCATCTC AGATATTTTG
    CAACAGCAAA ATACCCCCTT ACCCTCATTA ACACAGATGA CAGCCCCACC AGACCATTTG
    CCAAGCAATC AGTCAGAGAA CAGCCGAGCT GAGACCCTTT TAACCAACCC CCTGGGGAAC
    CCTTTACCAA GCTTTGCAGG CAGTGACACT ACTTTTAACC CCCTCTTCCT CCCAGCTGTC
    ACTGGGGCCT CAGGATTAAT GGCCTTGAAT CCCCAGCTGT TGGGAGGTGT CCTGAACTCG
    GCATCGGCCA ACACCGCTAA TCATCCAGAG GTTTCCATAG CAACTTCTTC CCAGGCAACC
    ACTACCACAA CCACTACATC ATCAGCAGTG GCAGCACTGA CTGTCTCAAC ACTTGGTGGG
    ACAGCAGTGG TGTCAATGGC AGAAACATTG CTGAATATAT CTAATAATGC TGGGAATACA
    CCTGGTCCAG CTAAACTCAA CAGTAACTCT GTGGTGCCAC AGCTACTTAA CCCTCTACTG
    GGGACAGGTC TGCTTGGTGA CATGCCCTCA ATAAACAGTA CTTTGAATAA CCATCAACTG
    ACTCATCTAC AGTCGCTGTT AAACAACAAT CAGATGTTTC CTCCAAATCA GCAGCAGCAG
    CAACTTCTTC AGGGGTACCA GAATCTCCAG GCATTTCAAG GACAGTCCAC AATTCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAATT TCCAGGTGAG AATGCAGGAA
    GATGCAGCTC TCCTAAACAA AAGAATAAGC ACTCAGCCGG GGCTCACAGC ACTTCCCGAA
    AATCCAAACA CTACACTTCC ACCTTTCCAA GACACCTCTT GTGAGTTGCA GTCGAGGATT
    GACCCATCTC TAGGTCAGCA GGTGAAGGAT GGCCTCATCA TGGGGGGCCA AGGCGATGCT
    TCCGTAGATG CTATTTACAA AGCAGTCGTG GATGCAGCCA GCAAAGGGAT GCAGGTTGTC
    ATCACCACTG CTGTCAACAG TACAACTCAG ATCAGCCCCA TTCCAGCTCT GAGTGCCATG
    AGTGCCTTCA CTGCTTCCAT CGGTGACCCA TTAAGTCTCC CCAGTGCTGT CAGTGCGGTC
    ATTCACGGAC GAAACATGGG CAGTGTGGAT CACGATGGGC GGCTGAGGAG TGCGAGAGGG
    GCTCGGCTGC CTAGCAGTCT GGACCACGGG AAGAACTCCA GCGAAGGAGA TGGCTTTGAA
    TATTTCAAAT CAGCAGGTTG CCACACATCT AAAAAACAGT GGGATGGGGA GCAAAGCCCT
    GGAGGGGAGC GAAACAGGTG GAAGTGTGAG GAATTTTTAG ATCATCCAGG CCACATCCAC
    AATAGTCCTT GTCACGAAAG GCCCAACAAT GTCTCTACAC TGCCATTTCT GCCTGGGGAA
    CAGCACCCAA TACTGTTACC ACCGAGAAAC TGTCAAGGGG ATAAGATTTT GGAGGAGAAT
    TTCAGGTATA ATAACTACAA AAGAACTATG ATGAGTTTTA AGGAGAGACT AGAGAACACT
    GTGGAAAGAT GCGCACACAT TAACGGGAAC AGATCTCGGC AGAGTCGGGG CTTTGGAGAG
    CTGCTGAGCA CCACAAAGCA AGACTTTGTC CTAGAGGAGC AATCTCCGAG CTCCTCCAAT
    AGTTTGGAGA GTTCTCTGGT CAAAGACTAC ATCCATTACA ATGGAGACTT TAATGCCAAA
    AACATTAACG GGTGTGTACC TAGCCCTTCA GACGCTAAAA GCATTAGTAG TGAAGATGAC
    CTAAGGAACC CAGACTCCCC CTCTTCAAAT GAATTGATCC CTTATAGGCC AAGGACGTTC
    AACGTTGGCG ACTTGGTCTG GGGCCAAATC AAAGGACTGA CTTCCTGGCC TGGAAAATTA
    GTCAGAGAAG ACGACGTTCA CAGTTCTTGT CAACAAAGCC CCGAGGAAGG GAAGGTGGAG
    CCCGAGAAGT TGAAGACACT AACGGAAGGT TTAGAAGCCT ACAGCCGAGC CCGGAAAAGG
    AACAGAAAAA GTGGAAAGCT AAATAACCAT TTAGAAGCTG CTATTCATGA GGCCATGAGT
    GAACTGGACA AAATGTCTGG GACTGTTCAC CAAATCCCCC AGGGAGACAG ACAGATGAGA
    CCTCCCAAAC CCAAGAGGAG GAAGATCTCC AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330921.1
    CDS859..6054
    Translation

    Target ORF information:

    RefSeq Version XM_015475435.2
    Organism Bos taurus(cattle)
    Definition Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475435.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGCA AGTCTGAGAT CAACCTCCAC
    CCTTTAGGTT TTCTCAACCC GAATGTAAAC GCTGCTTTAG CTTTTCTCTC CGGTGACATG
    GATGGGCAGG TATTGCAGCC TGTTCACTTT CAACTCTTAG CAGCCCTGCT TCAGAACCAA
    GCCCACGCAG CTGCCATGCT TCCCCTGCCA TCTTTCAATC TGGCCATCTC AGATATTTTG
    CAACAGCAAA ATACCCCCTT ACCCTCATTA ACACAGATGA CAGCCCCACC AGACCATTTG
    CCAAGCAATC AGTCAGAGAA CAGCCGAGCT GAGACCCTTT TAACCAACCC CCTGGGGAAC
    CCTTTACCAA GCTTTGCAGG CAGTGACACT ACTTTTAACC CCCTCTTCCT CCCAGCTGTC
    ACTGGGGCCT CAGGATTAAT GGCCTTGAAT CCCCAGCTGT TGGGAGGTGT CCTGAACTCG
    GCATCGGCCA ACACCGCTAA TCATCCAGAG GTTTCCATAG CAACTTCTTC CCAGGCAACC
    ACTACCACAA CCACTACATC ATCAGCAGTG GCAGCACTGA CTGTCTCAAC ACTTGGTGGG
    ACAGCAGTGG TGTCAATGGC AGAAACATTG CTGAATATAT CTAATAATGC TGGGAATACA
    CCTGGTCCAG CTAAACTCAA CAGTAACTCT GTGGTGCCAC AGCTACTTAA CCCTCTACTG
    GGGACAGGTC TGCTTGGTGA CATGCCCTCA ATAAACAGTA CTTTGAATAA CCATCAACTG
    ACTCATCTAC AGTCGCTGTT AAACAACAAT CAGATGTTTC CTCCAAATCA GCAGCAGCAG
    CAACTTCTTC AGGGGTACCA GAATCTCCAG GCATTTCAAG GACAGTCCAC AATTCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAATT TCCAGGTGAG AATGCAGGAA
    GATGCAGCTC TCCTAAACAA AAGAATAAGC ACTCAGCCGG GGCTCACAGC ACTTCCCGAA
    AATCCAAACA CTACACTTCC ACCTTTCCAA GACACCTCTT GTGAGTTGCA GTCGAGGATT
    GACCCATCTC TAGGTCAGCA GGTGAAGGAT GGCCTCATCA TGGGGGGCCA AGGCGATGCT
    TCCGTAGATG CTATTTACAA AGCAGTCGTG GATGCAGCCA GCAAAGGGAT GCAGGTTGTC
    ATCACCACTG CTGTCAACAG TACAACTCAG ATCAGCCCCA TTCCAGCTCT GAGTGCCATG
    AGTGCCTTCA CTGCTTCCAT CGGTGACCCA TTAAGTCTCC CCAGTGCTGT CAGTGCGGTC
    ATTCACGGAC GAAACATGGG CAGTGTGGAT CACGATGGGC GGCTGAGGAG TGCGAGAGGG
    GCTCGGCTGC CTAGCAGTCT GGACCACGGG AAGAACTCCA GCGAAGGAGA TGGCTTTGAA
    TATTTCAAAT CAGCAGGTTG CCACACATCT AAAAAACAGT GGGATGGGGA GCAAAGCCCT
    GGAGGGGAGC GAAACAGGTG GAAGTGTGAG GAATTTTTAG ATCATCCAGG CCACATCCAC
    AATAGTCCTT GTCACGAAAG GCCCAACAAT GTCTCTACAC TGCCATTTCT GCCTGGGGAA
    CAGCACCCAA TACTGTTACC ACCGAGAAAC TGTCAAGGGG ATAAGATTTT GGAGGAGAAT
    TTCAGGTATA ATAACTACAA AAGAACTATG ATGAGTTTTA AGGAGAGACT AGAGAACACT
    GTGGAAAGAT GCGCACACAT TAACGGGAAC AGATCTCGGC AGAGTCGGGG CTTTGGAGAG
    CTGCTGAGCA CCACAAAGCA AGACTTTGTC CTAGAGGAGC AATCTCCGAG CTCCTCCAAT
    AGTTTGGAGA GTTCTCTGGT CAAAGACTAC ATCCATTACA ATGGAGACTT TAATGCCAAA
    AACATTAACG GGTGTGTACC TAGCCCTTCA GACGCTAAAA GCATTAGTAG TGAAGATGAC
    CTAAGGAACC CAGACTCCCC CTCTTCAAAT GAATTGATCC CTTATAGGCC AAGGACGTTC
    AACGTTGGCG ACTTGGTCTG GGGCCAAATC AAAGGACTGA CTTCCTGGCC TGGAAAATTA
    GTCAGAGAAG ACGACGTTCA CAGTTCTTGT CAACAAAGCC CCGAGGAAGG GAAGGTGGAG
    CCCGAGAAGT TGAAGACACT AACGGAAGGT TTAGAAGCCT ACAGCCGAGC CCGGAAAAGG
    AACAGAAAAA GTGGAAAGCT AAATAACCAT TTAGAAGCTG CTATTCATGA GGCCATGAGT
    GAACTGGACA AAATGTCTGG GACTGTTCAC CAAATCCCCC AGGGAGACAG ACAGATGAGA
    CCTCCCAAAC CCAAGAGGAG GAAGATCTCC AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16449
    Clone ID Related Accession (Same CDS sequence) XM_015475445.1 , XM_015475445.2 , NM_001191268.1
    Accession Version XM_015475445.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product methyl-CpG-binding domain protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGTG ACATGCCCTC AATAAACAGT
    ACTTTGAATA ACCATCAACT GACTCATCTA CAGTCGCTGT TAAACAACAA TCAGATGTTT
    CCTCCAAATC AGCAGCAGCA GCAACTTCTT CAGGGGTACC AGAATCTCCA GGCATTTCAA
    GGACAGTCCA CAATTCCTTG CCCAGCTAAC AATAACCCCA TGGCTTGTCT GTTTCAGAAT
    TTCCAGGTGA GAATGCAGGA AGATGCAGCT CTCCTAAACA AAAGAATAAG CACTCAGCCG
    GGGCTCACAG CACTTCCCGA AAATCCAAAC ACTACACTTC CACCTTTCCA AGACACCTCT
    TGTGAGTTGC AGTCGAGGAT TGACCCATCT CTAGGTCAGC AGGTGAAGGA TGGCCTCATC
    ATGGGGGGCC AAGGCGATGC TTCCGTAGAT GCTATTTACA AAGCAGTCGT GGATGCAGCC
    AGCAAAGGGA TGCAGGTTGT CATCACCACT GCTGTCAACA GTACAACTCA GATCAGCCCC
    ATTCCAGCTC TGAGTGCCAT GAGTGCCTTC ACTGCTTCCA TCGGTGACCC ATTAAGTCTC
    CCCAGTGCTG TCAGTGCGGT CATTCACGGA CGAAACATGG GCAGTGTGGA TCACGATGGG
    CGGCTGAGGA GTGCGAGAGG GGCTCGGCTG CCTAGCAGTC TGGACCACGG GAAGAACTCC
    AGCGAAGGAG ATGGCTTTGA ATATTTCAAA TCAGCAGGTT GCCACACATC TAAAAAACAG
    TGGGATGGGG AGCAAAGCCC TGGAGGGGAG CGAAACAGGT GGAAGTGTGA GGAATTTTTA
    GATCATCCAG GCCACATCCA CAATAGTCCT TGTCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TGCCTGGGGA ACAGCACCCA ATACTGTTAC CACCGAGAAA CTGTCAAGGG
    GATAAGATTT TGGAGGAGAA TTTCAGGTAT AATAACTACA AAAGAACTAT GATGAGTTTT
    AAGGAGAGAC TAGAGAACAC TGTGGAAAGA TGCGCACACA TTAACGGGAA CAGATCTCGG
    CAGAGTCGGG GCTTTGGAGA GCTGCTGAGC ACCACAAAGC AAGACTTTGT CCTAGAGGAG
    CAATCTCCGA GCTCCTCCAA TAGTTTGGAG AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AAACATTAAC GGGTGTGTAC CTAGCCCTTC AGACGCTAAA
    AGCATTAGTA GTGAAGATGA CCTAAGGAAC CCAGACTCCC CCTCTTCAAA TGAATTGATC
    CCTTATAGGC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGAAAATT AGTCAGAGAA GACGACGTTC ACAGTTCTTG TCAACAAAGC
    CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG TTGAAGACAC TAACGGAAGG TTTAGAAGCC
    TACAGCCGAG CCCGGAAAAG GAACAGAAAA AGTGGAAAGC TAAATAACCA TTTAGAAGCT
    GCTATTCATG AGGCCATGAG TGAACTGGAC AAAATGTCTG GGACTGTTCA CCAAATCCCC
    CAGGGAGACA GACAGATGAG ACCTCCCAAA CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330931.1
    CDS857..5353
    Translation

    Target ORF information:

    RefSeq Version XM_015475445.1
    Organism Bos taurus(cattle)
    Definition Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475445.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGTG ACATGCCCTC AATAAACAGT
    ACTTTGAATA ACCATCAACT GACTCATCTA CAGTCGCTGT TAAACAACAA TCAGATGTTT
    CCTCCAAATC AGCAGCAGCA GCAACTTCTT CAGGGGTACC AGAATCTCCA GGCATTTCAA
    GGACAGTCCA CAATTCCTTG CCCAGCTAAC AATAACCCCA TGGCTTGTCT GTTTCAGAAT
    TTCCAGGTGA GAATGCAGGA AGATGCAGCT CTCCTAAACA AAAGAATAAG CACTCAGCCG
    GGGCTCACAG CACTTCCCGA AAATCCAAAC ACTACACTTC CACCTTTCCA AGACACCTCT
    TGTGAGTTGC AGTCGAGGAT TGACCCATCT CTAGGTCAGC AGGTGAAGGA TGGCCTCATC
    ATGGGGGGCC AAGGCGATGC TTCCGTAGAT GCTATTTACA AAGCAGTCGT GGATGCAGCC
    AGCAAAGGGA TGCAGGTTGT CATCACCACT GCTGTCAACA GTACAACTCA GATCAGCCCC
    ATTCCAGCTC TGAGTGCCAT GAGTGCCTTC ACTGCTTCCA TCGGTGACCC ATTAAGTCTC
    CCCAGTGCTG TCAGTGCGGT CATTCACGGA CGAAACATGG GCAGTGTGGA TCACGATGGG
    CGGCTGAGGA GTGCGAGAGG GGCTCGGCTG CCTAGCAGTC TGGACCACGG GAAGAACTCC
    AGCGAAGGAG ATGGCTTTGA ATATTTCAAA TCAGCAGGTT GCCACACATC TAAAAAACAG
    TGGGATGGGG AGCAAAGCCC TGGAGGGGAG CGAAACAGGT GGAAGTGTGA GGAATTTTTA
    GATCATCCAG GCCACATCCA CAATAGTCCT TGTCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TGCCTGGGGA ACAGCACCCA ATACTGTTAC CACCGAGAAA CTGTCAAGGG
    GATAAGATTT TGGAGGAGAA TTTCAGGTAT AATAACTACA AAAGAACTAT GATGAGTTTT
    AAGGAGAGAC TAGAGAACAC TGTGGAAAGA TGCGCACACA TTAACGGGAA CAGATCTCGG
    CAGAGTCGGG GCTTTGGAGA GCTGCTGAGC ACCACAAAGC AAGACTTTGT CCTAGAGGAG
    CAATCTCCGA GCTCCTCCAA TAGTTTGGAG AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AAACATTAAC GGGTGTGTAC CTAGCCCTTC AGACGCTAAA
    AGCATTAGTA GTGAAGATGA CCTAAGGAAC CCAGACTCCC CCTCTTCAAA TGAATTGATC
    CCTTATAGGC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGAAAATT AGTCAGAGAA GACGACGTTC ACAGTTCTTG TCAACAAAGC
    CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG TTGAAGACAC TAACGGAAGG TTTAGAAGCC
    TACAGCCGAG CCCGGAAAAG GAACAGAAAA AGTGGAAAGC TAAATAACCA TTTAGAAGCT
    GCTATTCATG AGGCCATGAG TGAACTGGAC AAAATGTCTG GGACTGTTCA CCAAATCCCC
    CAGGGAGACA GACAGATGAG ACCTCCCAAA CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16449
    Clone ID Related Accession (Same CDS sequence) XM_015475445.1 , XM_015475445.2 , NM_001191268.1
    Accession Version XM_015475445.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product methyl-CpG-binding domain protein 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475445.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGTG ACATGCCCTC AATAAACAGT
    ACTTTGAATA ACCATCAACT GACTCATCTA CAGTCGCTGT TAAACAACAA TCAGATGTTT
    CCTCCAAATC AGCAGCAGCA GCAACTTCTT CAGGGGTACC AGAATCTCCA GGCATTTCAA
    GGACAGTCCA CAATTCCTTG CCCAGCTAAC AATAACCCCA TGGCTTGTCT GTTTCAGAAT
    TTCCAGGTGA GAATGCAGGA AGATGCAGCT CTCCTAAACA AAAGAATAAG CACTCAGCCG
    GGGCTCACAG CACTTCCCGA AAATCCAAAC ACTACACTTC CACCTTTCCA AGACACCTCT
    TGTGAGTTGC AGTCGAGGAT TGACCCATCT CTAGGTCAGC AGGTGAAGGA TGGCCTCATC
    ATGGGGGGCC AAGGCGATGC TTCCGTAGAT GCTATTTACA AAGCAGTCGT GGATGCAGCC
    AGCAAAGGGA TGCAGGTTGT CATCACCACT GCTGTCAACA GTACAACTCA GATCAGCCCC
    ATTCCAGCTC TGAGTGCCAT GAGTGCCTTC ACTGCTTCCA TCGGTGACCC ATTAAGTCTC
    CCCAGTGCTG TCAGTGCGGT CATTCACGGA CGAAACATGG GCAGTGTGGA TCACGATGGG
    CGGCTGAGGA GTGCGAGAGG GGCTCGGCTG CCTAGCAGTC TGGACCACGG GAAGAACTCC
    AGCGAAGGAG ATGGCTTTGA ATATTTCAAA TCAGCAGGTT GCCACACATC TAAAAAACAG
    TGGGATGGGG AGCAAAGCCC TGGAGGGGAG CGAAACAGGT GGAAGTGTGA GGAATTTTTA
    GATCATCCAG GCCACATCCA CAATAGTCCT TGTCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TGCCTGGGGA ACAGCACCCA ATACTGTTAC CACCGAGAAA CTGTCAAGGG
    GATAAGATTT TGGAGGAGAA TTTCAGGTAT AATAACTACA AAAGAACTAT GATGAGTTTT
    AAGGAGAGAC TAGAGAACAC TGTGGAAAGA TGCGCACACA TTAACGGGAA CAGATCTCGG
    CAGAGTCGGG GCTTTGGAGA GCTGCTGAGC ACCACAAAGC AAGACTTTGT CCTAGAGGAG
    CAATCTCCGA GCTCCTCCAA TAGTTTGGAG AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AAACATTAAC GGGTGTGTAC CTAGCCCTTC AGACGCTAAA
    AGCATTAGTA GTGAAGATGA CCTAAGGAAC CCAGACTCCC CCTCTTCAAA TGAATTGATC
    CCTTATAGGC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGAAAATT AGTCAGAGAA GACGACGTTC ACAGTTCTTG TCAACAAAGC
    CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG TTGAAGACAC TAACGGAAGG TTTAGAAGCC
    TACAGCCGAG CCCGGAAAAG GAACAGAAAA AGTGGAAAGC TAAATAACCA TTTAGAAGCT
    GCTATTCATG AGGCCATGAG TGAACTGGAC AAAATGTCTG GGACTGTTCA CCAAATCCCC
    CAGGGAGACA GACAGATGAG ACCTCCCAAA CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330931.1
    CDS859..5355
    Translation

    Target ORF information:

    RefSeq Version XM_015475445.2
    Organism Bos taurus(cattle)
    Definition Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475445.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGTG ACATGCCCTC AATAAACAGT
    ACTTTGAATA ACCATCAACT GACTCATCTA CAGTCGCTGT TAAACAACAA TCAGATGTTT
    CCTCCAAATC AGCAGCAGCA GCAACTTCTT CAGGGGTACC AGAATCTCCA GGCATTTCAA
    GGACAGTCCA CAATTCCTTG CCCAGCTAAC AATAACCCCA TGGCTTGTCT GTTTCAGAAT
    TTCCAGGTGA GAATGCAGGA AGATGCAGCT CTCCTAAACA AAAGAATAAG CACTCAGCCG
    GGGCTCACAG CACTTCCCGA AAATCCAAAC ACTACACTTC CACCTTTCCA AGACACCTCT
    TGTGAGTTGC AGTCGAGGAT TGACCCATCT CTAGGTCAGC AGGTGAAGGA TGGCCTCATC
    ATGGGGGGCC AAGGCGATGC TTCCGTAGAT GCTATTTACA AAGCAGTCGT GGATGCAGCC
    AGCAAAGGGA TGCAGGTTGT CATCACCACT GCTGTCAACA GTACAACTCA GATCAGCCCC
    ATTCCAGCTC TGAGTGCCAT GAGTGCCTTC ACTGCTTCCA TCGGTGACCC ATTAAGTCTC
    CCCAGTGCTG TCAGTGCGGT CATTCACGGA CGAAACATGG GCAGTGTGGA TCACGATGGG
    CGGCTGAGGA GTGCGAGAGG GGCTCGGCTG CCTAGCAGTC TGGACCACGG GAAGAACTCC
    AGCGAAGGAG ATGGCTTTGA ATATTTCAAA TCAGCAGGTT GCCACACATC TAAAAAACAG
    TGGGATGGGG AGCAAAGCCC TGGAGGGGAG CGAAACAGGT GGAAGTGTGA GGAATTTTTA
    GATCATCCAG GCCACATCCA CAATAGTCCT TGTCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TGCCTGGGGA ACAGCACCCA ATACTGTTAC CACCGAGAAA CTGTCAAGGG
    GATAAGATTT TGGAGGAGAA TTTCAGGTAT AATAACTACA AAAGAACTAT GATGAGTTTT
    AAGGAGAGAC TAGAGAACAC TGTGGAAAGA TGCGCACACA TTAACGGGAA CAGATCTCGG
    CAGAGTCGGG GCTTTGGAGA GCTGCTGAGC ACCACAAAGC AAGACTTTGT CCTAGAGGAG
    CAATCTCCGA GCTCCTCCAA TAGTTTGGAG AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AAACATTAAC GGGTGTGTAC CTAGCCCTTC AGACGCTAAA
    AGCATTAGTA GTGAAGATGA CCTAAGGAAC CCAGACTCCC CCTCTTCAAA TGAATTGATC
    CCTTATAGGC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGAAAATT AGTCAGAGAA GACGACGTTC ACAGTTCTTG TCAACAAAGC
    CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG TTGAAGACAC TAACGGAAGG TTTAGAAGCC
    TACAGCCGAG CCCGGAAAAG GAACAGAAAA AGTGGAAAGC TAAATAACCA TTTAGAAGCT
    GCTATTCATG AGGCCATGAG TGAACTGGAC AAAATGTCTG GGACTGTTCA CCAAATCCCC
    CAGGGAGACA GACAGATGAG ACCTCCCAAA CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54961
    Clone ID Related Accession (Same CDS sequence) XM_015475434.2 , XM_015475432.1 , XM_015475432.2 , XM_015475434.1
    Accession Version XM_015475434.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475434.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGCA AGTCTGAGAT CAACCTCCAC
    CCTTTAGGTT TTCTCAACCC GAATGTAAAC GCTGCTTTAG CTTTTCTCTC CGGTGACATG
    GATGGGCAGG TATTGCAGCC TGTTCACTTT CAACTCTTAG CAGCCCTGCT TCAGAACCAA
    GCCCACGCAG CTGCCATGCT TCCCCTGCCA TCTTTCAATC TGGCCATCTC AGATATTTTG
    CAACAGCAAA ATACCCCCTT ACCCTCATTA ACACAGATGA CAGCCCCACC AGACCATTTG
    CCAAGCAATC AGTCAGAGAA CAGCCGAGCT GAGACCCTTT TAACCAACCC CCTGGGGAAC
    CCTTTACCAA GCTTTGCAGG CAGTGACACT ACTTTTAACC CCCTCTTCCT CCCAGCTGTC
    ACTGGGGCCT CAGGATTAAT GGCCTTGAAT CCCCAGCTGT TGGGAGGTGT CCTGAACTCG
    GCATCGGCCA ACACCGCTAA TCATCCAGAG GTTTCCATAG CAACTTCTTC CCAGGCAACC
    ACTACCACAA CCACTACATC ATCAGCAGTG GCAGCACTGA CTGTCTCAAC ACTTGGTGGG
    ACAGCAGTGG TGTCAATGGC AGAAACATTG CTGAATATAT CTAATAATGC TGGGAATACA
    CCTGGTCCAG CTAAACTCAA CAGTAACTCT GTGGTGCCAC AGCTACTTAA CCCTCTACTG
    GGGACAGGTC TGCTTGGTGA CATGCCCTCA ATAAACAGTA CTTTGAATAA CCATCAACTG
    ACTCATCTAC AGTCGCTGTT AAACAACAAT CAGATGTTTC CTCCAAATCA GCAGCAGCAG
    CAACTTCTTC AGGGGTACCA GAATCTCCAG GCATTTCAAG GACAGTCCAC AATTCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAATT TCCAGGTGAG AATGCAGGAA
    GATGCAGCTC TCCTAAACAA AAGAATAAGC ACTCAGCCGG GGCTCACAGC ACTTCCCGAA
    AATCCAAACA CTACACTTCC ACCTTTCCAA GACACCTCTT GTGAGTTGCA GTCGAGGATT
    GACCCATCTC TAGGTCAGCA GGTGAAGGAT GGCCTCATCA TGGGGGGCCA AGGCGATGCT
    TCCGTAGATG CTATTTACAA AGCAGTCGTG GATGCAGCCA GCAAAGGGAT GCAGGTTGTC
    ATCACCACTG CTGTCAACAG TACAACTCAG ATCAGCCCCA TTCCAGCTCT GAGTGCCATG
    AGTGCCTTCA CTGCTTCCAT CGGTGACCCA TTAAGTCTCC CCAGTGCTGT CAGTGCGGTC
    ATTCACGGAC GAAACATGGG CAGTGTGGAT CACGATGGGC GGCTGAGGAG TGCGAGAGGG
    GCTCGGCTGC CTAGCAGTCT GGACCACGGG AAGAACTCCA GCGAAGGAGA TGGCTTTGAA
    TATTTCAAAT CAGCAGGTTG CCACACATCT AAAAAACAGT GGGATGGGGA GCAAAGCCCT
    GGAGGGGAGC GAAACAGGTG GAAGTGTGAG GAATTTTTAG ATCATCCAGG CCACATCCAC
    AATAGTCCTT GTCACGAAAG GCCCAACAAT GTCTCTACAC TGCCATTTCT GCCTGGGGAA
    CAGCACCCAA TACTGTTACC ACCGAGAAAC TGTCAAGGGG ATAAGATTTT GGAGGAGAAT
    TTCAGGTATA ATAACTACAA AAGAACTATG ATGAGTTTTA AGGAGAGACT AGAGAACACT
    GTGGAAAGAT GCGCACACAT TAACGGGAAC AGATCTCGGC AGAGTCGGGG CTTTGGAGAG
    CTGCTGAGCA CCACAAAGCA AGACTTTGTC CTAGAGGAGC AATCTCCGAG CTCCTCCAAT
    AGTTTGGAGA GTTCTCTGGT CAAAGACTAC ATCCATTACA ATGGAGACTT TAATGCCAAA
    AACATTAACG GGTGTGTACC TAGCCCTTCA GACGCTAAAA GCATTAGTAG TGAAGATGAC
    CTAAGGAACC CAGACTCCCC CTCTTCAAAT GAATTGATCC CTTATAGGCC AAGGACGTTC
    AACGTTGGCG ACTTGGTCTG GGGCCAAATC AAAGGACTGA CTTCCTGGCC TGGAAAATTA
    GTCAGAGAAG ACGACGTTCA CAGTTCTTGT CAACAAAGCC CCGAGGAAGG GAAGGTCTGG
    GTAATGTGGT CTGGTCTTCA TGCCTTCACT CAGGTGGAGC CCGAGAAGTT GAAGACACTA
    ACGGAAGGTT TAGAAGCCTA CAGCCGAGCC CGGAAAAGGA ACAGAAAAAG TGGAAAGCTA
    AATAACCATT TAGAAGCTGC TATTCATGAG GCCATGAGTG AACTGGACAA AATGTCTGGG
    ACTGTTCACC AAATCCCCCA GGGAGACAGA CAGATGAGAC CTCCCAAACC CAAGAGGAGG
    AAGATCTCCA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330920.1
    CDS679..5913
    Translation

    Target ORF information:

    RefSeq Version XM_015475434.2
    Organism Bos taurus(cattle)
    Definition Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475434.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGCA AGTCTGAGAT CAACCTCCAC
    CCTTTAGGTT TTCTCAACCC GAATGTAAAC GCTGCTTTAG CTTTTCTCTC CGGTGACATG
    GATGGGCAGG TATTGCAGCC TGTTCACTTT CAACTCTTAG CAGCCCTGCT TCAGAACCAA
    GCCCACGCAG CTGCCATGCT TCCCCTGCCA TCTTTCAATC TGGCCATCTC AGATATTTTG
    CAACAGCAAA ATACCCCCTT ACCCTCATTA ACACAGATGA CAGCCCCACC AGACCATTTG
    CCAAGCAATC AGTCAGAGAA CAGCCGAGCT GAGACCCTTT TAACCAACCC CCTGGGGAAC
    CCTTTACCAA GCTTTGCAGG CAGTGACACT ACTTTTAACC CCCTCTTCCT CCCAGCTGTC
    ACTGGGGCCT CAGGATTAAT GGCCTTGAAT CCCCAGCTGT TGGGAGGTGT CCTGAACTCG
    GCATCGGCCA ACACCGCTAA TCATCCAGAG GTTTCCATAG CAACTTCTTC CCAGGCAACC
    ACTACCACAA CCACTACATC ATCAGCAGTG GCAGCACTGA CTGTCTCAAC ACTTGGTGGG
    ACAGCAGTGG TGTCAATGGC AGAAACATTG CTGAATATAT CTAATAATGC TGGGAATACA
    CCTGGTCCAG CTAAACTCAA CAGTAACTCT GTGGTGCCAC AGCTACTTAA CCCTCTACTG
    GGGACAGGTC TGCTTGGTGA CATGCCCTCA ATAAACAGTA CTTTGAATAA CCATCAACTG
    ACTCATCTAC AGTCGCTGTT AAACAACAAT CAGATGTTTC CTCCAAATCA GCAGCAGCAG
    CAACTTCTTC AGGGGTACCA GAATCTCCAG GCATTTCAAG GACAGTCCAC AATTCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAATT TCCAGGTGAG AATGCAGGAA
    GATGCAGCTC TCCTAAACAA AAGAATAAGC ACTCAGCCGG GGCTCACAGC ACTTCCCGAA
    AATCCAAACA CTACACTTCC ACCTTTCCAA GACACCTCTT GTGAGTTGCA GTCGAGGATT
    GACCCATCTC TAGGTCAGCA GGTGAAGGAT GGCCTCATCA TGGGGGGCCA AGGCGATGCT
    TCCGTAGATG CTATTTACAA AGCAGTCGTG GATGCAGCCA GCAAAGGGAT GCAGGTTGTC
    ATCACCACTG CTGTCAACAG TACAACTCAG ATCAGCCCCA TTCCAGCTCT GAGTGCCATG
    AGTGCCTTCA CTGCTTCCAT CGGTGACCCA TTAAGTCTCC CCAGTGCTGT CAGTGCGGTC
    ATTCACGGAC GAAACATGGG CAGTGTGGAT CACGATGGGC GGCTGAGGAG TGCGAGAGGG
    GCTCGGCTGC CTAGCAGTCT GGACCACGGG AAGAACTCCA GCGAAGGAGA TGGCTTTGAA
    TATTTCAAAT CAGCAGGTTG CCACACATCT AAAAAACAGT GGGATGGGGA GCAAAGCCCT
    GGAGGGGAGC GAAACAGGTG GAAGTGTGAG GAATTTTTAG ATCATCCAGG CCACATCCAC
    AATAGTCCTT GTCACGAAAG GCCCAACAAT GTCTCTACAC TGCCATTTCT GCCTGGGGAA
    CAGCACCCAA TACTGTTACC ACCGAGAAAC TGTCAAGGGG ATAAGATTTT GGAGGAGAAT
    TTCAGGTATA ATAACTACAA AAGAACTATG ATGAGTTTTA AGGAGAGACT AGAGAACACT
    GTGGAAAGAT GCGCACACAT TAACGGGAAC AGATCTCGGC AGAGTCGGGG CTTTGGAGAG
    CTGCTGAGCA CCACAAAGCA AGACTTTGTC CTAGAGGAGC AATCTCCGAG CTCCTCCAAT
    AGTTTGGAGA GTTCTCTGGT CAAAGACTAC ATCCATTACA ATGGAGACTT TAATGCCAAA
    AACATTAACG GGTGTGTACC TAGCCCTTCA GACGCTAAAA GCATTAGTAG TGAAGATGAC
    CTAAGGAACC CAGACTCCCC CTCTTCAAAT GAATTGATCC CTTATAGGCC AAGGACGTTC
    AACGTTGGCG ACTTGGTCTG GGGCCAAATC AAAGGACTGA CTTCCTGGCC TGGAAAATTA
    GTCAGAGAAG ACGACGTTCA CAGTTCTTGT CAACAAAGCC CCGAGGAAGG GAAGGTCTGG
    GTAATGTGGT CTGGTCTTCA TGCCTTCACT CAGGTGGAGC CCGAGAAGTT GAAGACACTA
    ACGGAAGGTT TAGAAGCCTA CAGCCGAGCC CGGAAAAGGA ACAGAAAAAG TGGAAAGCTA
    AATAACCATT TAGAAGCTGC TATTCATGAG GCCATGAGTG AACTGGACAA AATGTCTGGG
    ACTGTTCACC AAATCCCCCA GGGAGACAGA CAGATGAGAC CTCCCAAACC CAAGAGGAGG
    AAGATCTCCA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54961
    Clone ID Related Accession (Same CDS sequence) XM_015475434.2 , XM_015475432.1 , XM_015475432.2 , XM_015475434.1
    Accession Version XM_015475432.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGCA AGTCTGAGAT CAACCTCCAC
    CCTTTAGGTT TTCTCAACCC GAATGTAAAC GCTGCTTTAG CTTTTCTCTC CGGTGACATG
    GATGGGCAGG TATTGCAGCC TGTTCACTTT CAACTCTTAG CAGCCCTGCT TCAGAACCAA
    GCCCACGCAG CTGCCATGCT TCCCCTGCCA TCTTTCAATC TGGCCATCTC AGATATTTTG
    CAACAGCAAA ATACCCCCTT ACCCTCATTA ACACAGATGA CAGCCCCACC AGACCATTTG
    CCAAGCAATC AGTCAGAGAA CAGCCGAGCT GAGACCCTTT TAACCAACCC CCTGGGGAAC
    CCTTTACCAA GCTTTGCAGG CAGTGACACT ACTTTTAACC CCCTCTTCCT CCCAGCTGTC
    ACTGGGGCCT CAGGATTAAT GGCCTTGAAT CCCCAGCTGT TGGGAGGTGT CCTGAACTCG
    GCATCGGCCA ACACCGCTAA TCATCCAGAG GTTTCCATAG CAACTTCTTC CCAGGCAACC
    ACTACCACAA CCACTACATC ATCAGCAGTG GCAGCACTGA CTGTCTCAAC ACTTGGTGGG
    ACAGCAGTGG TGTCAATGGC AGAAACATTG CTGAATATAT CTAATAATGC TGGGAATACA
    CCTGGTCCAG CTAAACTCAA CAGTAACTCT GTGGTGCCAC AGCTACTTAA CCCTCTACTG
    GGGACAGGTC TGCTTGGTGA CATGCCCTCA ATAAACAGTA CTTTGAATAA CCATCAACTG
    ACTCATCTAC AGTCGCTGTT AAACAACAAT CAGATGTTTC CTCCAAATCA GCAGCAGCAG
    CAACTTCTTC AGGGGTACCA GAATCTCCAG GCATTTCAAG GACAGTCCAC AATTCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAATT TCCAGGTGAG AATGCAGGAA
    GATGCAGCTC TCCTAAACAA AAGAATAAGC ACTCAGCCGG GGCTCACAGC ACTTCCCGAA
    AATCCAAACA CTACACTTCC ACCTTTCCAA GACACCTCTT GTGAGTTGCA GTCGAGGATT
    GACCCATCTC TAGGTCAGCA GGTGAAGGAT GGCCTCATCA TGGGGGGCCA AGGCGATGCT
    TCCGTAGATG CTATTTACAA AGCAGTCGTG GATGCAGCCA GCAAAGGGAT GCAGGTTGTC
    ATCACCACTG CTGTCAACAG TACAACTCAG ATCAGCCCCA TTCCAGCTCT GAGTGCCATG
    AGTGCCTTCA CTGCTTCCAT CGGTGACCCA TTAAGTCTCC CCAGTGCTGT CAGTGCGGTC
    ATTCACGGAC GAAACATGGG CAGTGTGGAT CACGATGGGC GGCTGAGGAG TGCGAGAGGG
    GCTCGGCTGC CTAGCAGTCT GGACCACGGG AAGAACTCCA GCGAAGGAGA TGGCTTTGAA
    TATTTCAAAT CAGCAGGTTG CCACACATCT AAAAAACAGT GGGATGGGGA GCAAAGCCCT
    GGAGGGGAGC GAAACAGGTG GAAGTGTGAG GAATTTTTAG ATCATCCAGG CCACATCCAC
    AATAGTCCTT GTCACGAAAG GCCCAACAAT GTCTCTACAC TGCCATTTCT GCCTGGGGAA
    CAGCACCCAA TACTGTTACC ACCGAGAAAC TGTCAAGGGG ATAAGATTTT GGAGGAGAAT
    TTCAGGTATA ATAACTACAA AAGAACTATG ATGAGTTTTA AGGAGAGACT AGAGAACACT
    GTGGAAAGAT GCGCACACAT TAACGGGAAC AGATCTCGGC AGAGTCGGGG CTTTGGAGAG
    CTGCTGAGCA CCACAAAGCA AGACTTTGTC CTAGAGGAGC AATCTCCGAG CTCCTCCAAT
    AGTTTGGAGA GTTCTCTGGT CAAAGACTAC ATCCATTACA ATGGAGACTT TAATGCCAAA
    AACATTAACG GGTGTGTACC TAGCCCTTCA GACGCTAAAA GCATTAGTAG TGAAGATGAC
    CTAAGGAACC CAGACTCCCC CTCTTCAAAT GAATTGATCC CTTATAGGCC AAGGACGTTC
    AACGTTGGCG ACTTGGTCTG GGGCCAAATC AAAGGACTGA CTTCCTGGCC TGGAAAATTA
    GTCAGAGAAG ACGACGTTCA CAGTTCTTGT CAACAAAGCC CCGAGGAAGG GAAGGTCTGG
    GTAATGTGGT CTGGTCTTCA TGCCTTCACT CAGGTGGAGC CCGAGAAGTT GAAGACACTA
    ACGGAAGGTT TAGAAGCCTA CAGCCGAGCC CGGAAAAGGA ACAGAAAAAG TGGAAAGCTA
    AATAACCATT TAGAAGCTGC TATTCATGAG GCCATGAGTG AACTGGACAA AATGTCTGGG
    ACTGTTCACC AAATCCCCCA GGGAGACAGA CAGATGAGAC CTCCCAAACC CAAGAGGAGG
    AAGATCTCCA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330918.1
    CDS857..6091
    Translation

    Target ORF information:

    RefSeq Version XM_015475432.1
    Organism Bos taurus(cattle)
    Definition Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475432.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGCA AGTCTGAGAT CAACCTCCAC
    CCTTTAGGTT TTCTCAACCC GAATGTAAAC GCTGCTTTAG CTTTTCTCTC CGGTGACATG
    GATGGGCAGG TATTGCAGCC TGTTCACTTT CAACTCTTAG CAGCCCTGCT TCAGAACCAA
    GCCCACGCAG CTGCCATGCT TCCCCTGCCA TCTTTCAATC TGGCCATCTC AGATATTTTG
    CAACAGCAAA ATACCCCCTT ACCCTCATTA ACACAGATGA CAGCCCCACC AGACCATTTG
    CCAAGCAATC AGTCAGAGAA CAGCCGAGCT GAGACCCTTT TAACCAACCC CCTGGGGAAC
    CCTTTACCAA GCTTTGCAGG CAGTGACACT ACTTTTAACC CCCTCTTCCT CCCAGCTGTC
    ACTGGGGCCT CAGGATTAAT GGCCTTGAAT CCCCAGCTGT TGGGAGGTGT CCTGAACTCG
    GCATCGGCCA ACACCGCTAA TCATCCAGAG GTTTCCATAG CAACTTCTTC CCAGGCAACC
    ACTACCACAA CCACTACATC ATCAGCAGTG GCAGCACTGA CTGTCTCAAC ACTTGGTGGG
    ACAGCAGTGG TGTCAATGGC AGAAACATTG CTGAATATAT CTAATAATGC TGGGAATACA
    CCTGGTCCAG CTAAACTCAA CAGTAACTCT GTGGTGCCAC AGCTACTTAA CCCTCTACTG
    GGGACAGGTC TGCTTGGTGA CATGCCCTCA ATAAACAGTA CTTTGAATAA CCATCAACTG
    ACTCATCTAC AGTCGCTGTT AAACAACAAT CAGATGTTTC CTCCAAATCA GCAGCAGCAG
    CAACTTCTTC AGGGGTACCA GAATCTCCAG GCATTTCAAG GACAGTCCAC AATTCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAATT TCCAGGTGAG AATGCAGGAA
    GATGCAGCTC TCCTAAACAA AAGAATAAGC ACTCAGCCGG GGCTCACAGC ACTTCCCGAA
    AATCCAAACA CTACACTTCC ACCTTTCCAA GACACCTCTT GTGAGTTGCA GTCGAGGATT
    GACCCATCTC TAGGTCAGCA GGTGAAGGAT GGCCTCATCA TGGGGGGCCA AGGCGATGCT
    TCCGTAGATG CTATTTACAA AGCAGTCGTG GATGCAGCCA GCAAAGGGAT GCAGGTTGTC
    ATCACCACTG CTGTCAACAG TACAACTCAG ATCAGCCCCA TTCCAGCTCT GAGTGCCATG
    AGTGCCTTCA CTGCTTCCAT CGGTGACCCA TTAAGTCTCC CCAGTGCTGT CAGTGCGGTC
    ATTCACGGAC GAAACATGGG CAGTGTGGAT CACGATGGGC GGCTGAGGAG TGCGAGAGGG
    GCTCGGCTGC CTAGCAGTCT GGACCACGGG AAGAACTCCA GCGAAGGAGA TGGCTTTGAA
    TATTTCAAAT CAGCAGGTTG CCACACATCT AAAAAACAGT GGGATGGGGA GCAAAGCCCT
    GGAGGGGAGC GAAACAGGTG GAAGTGTGAG GAATTTTTAG ATCATCCAGG CCACATCCAC
    AATAGTCCTT GTCACGAAAG GCCCAACAAT GTCTCTACAC TGCCATTTCT GCCTGGGGAA
    CAGCACCCAA TACTGTTACC ACCGAGAAAC TGTCAAGGGG ATAAGATTTT GGAGGAGAAT
    TTCAGGTATA ATAACTACAA AAGAACTATG ATGAGTTTTA AGGAGAGACT AGAGAACACT
    GTGGAAAGAT GCGCACACAT TAACGGGAAC AGATCTCGGC AGAGTCGGGG CTTTGGAGAG
    CTGCTGAGCA CCACAAAGCA AGACTTTGTC CTAGAGGAGC AATCTCCGAG CTCCTCCAAT
    AGTTTGGAGA GTTCTCTGGT CAAAGACTAC ATCCATTACA ATGGAGACTT TAATGCCAAA
    AACATTAACG GGTGTGTACC TAGCCCTTCA GACGCTAAAA GCATTAGTAG TGAAGATGAC
    CTAAGGAACC CAGACTCCCC CTCTTCAAAT GAATTGATCC CTTATAGGCC AAGGACGTTC
    AACGTTGGCG ACTTGGTCTG GGGCCAAATC AAAGGACTGA CTTCCTGGCC TGGAAAATTA
    GTCAGAGAAG ACGACGTTCA CAGTTCTTGT CAACAAAGCC CCGAGGAAGG GAAGGTCTGG
    GTAATGTGGT CTGGTCTTCA TGCCTTCACT CAGGTGGAGC CCGAGAAGTT GAAGACACTA
    ACGGAAGGTT TAGAAGCCTA CAGCCGAGCC CGGAAAAGGA ACAGAAAAAG TGGAAAGCTA
    AATAACCATT TAGAAGCTGC TATTCATGAG GCCATGAGTG AACTGGACAA AATGTCTGGG
    ACTGTTCACC AAATCCCCCA GGGAGACAGA CAGATGAGAC CTCCCAAACC CAAGAGGAGG
    AAGATCTCCA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54961
    Clone ID Related Accession (Same CDS sequence) XM_015475434.2 , XM_015475432.1 , XM_015475432.2 , XM_015475434.1
    Accession Version XM_015475432.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475432.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGCA AGTCTGAGAT CAACCTCCAC
    CCTTTAGGTT TTCTCAACCC GAATGTAAAC GCTGCTTTAG CTTTTCTCTC CGGTGACATG
    GATGGGCAGG TATTGCAGCC TGTTCACTTT CAACTCTTAG CAGCCCTGCT TCAGAACCAA
    GCCCACGCAG CTGCCATGCT TCCCCTGCCA TCTTTCAATC TGGCCATCTC AGATATTTTG
    CAACAGCAAA ATACCCCCTT ACCCTCATTA ACACAGATGA CAGCCCCACC AGACCATTTG
    CCAAGCAATC AGTCAGAGAA CAGCCGAGCT GAGACCCTTT TAACCAACCC CCTGGGGAAC
    CCTTTACCAA GCTTTGCAGG CAGTGACACT ACTTTTAACC CCCTCTTCCT CCCAGCTGTC
    ACTGGGGCCT CAGGATTAAT GGCCTTGAAT CCCCAGCTGT TGGGAGGTGT CCTGAACTCG
    GCATCGGCCA ACACCGCTAA TCATCCAGAG GTTTCCATAG CAACTTCTTC CCAGGCAACC
    ACTACCACAA CCACTACATC ATCAGCAGTG GCAGCACTGA CTGTCTCAAC ACTTGGTGGG
    ACAGCAGTGG TGTCAATGGC AGAAACATTG CTGAATATAT CTAATAATGC TGGGAATACA
    CCTGGTCCAG CTAAACTCAA CAGTAACTCT GTGGTGCCAC AGCTACTTAA CCCTCTACTG
    GGGACAGGTC TGCTTGGTGA CATGCCCTCA ATAAACAGTA CTTTGAATAA CCATCAACTG
    ACTCATCTAC AGTCGCTGTT AAACAACAAT CAGATGTTTC CTCCAAATCA GCAGCAGCAG
    CAACTTCTTC AGGGGTACCA GAATCTCCAG GCATTTCAAG GACAGTCCAC AATTCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAATT TCCAGGTGAG AATGCAGGAA
    GATGCAGCTC TCCTAAACAA AAGAATAAGC ACTCAGCCGG GGCTCACAGC ACTTCCCGAA
    AATCCAAACA CTACACTTCC ACCTTTCCAA GACACCTCTT GTGAGTTGCA GTCGAGGATT
    GACCCATCTC TAGGTCAGCA GGTGAAGGAT GGCCTCATCA TGGGGGGCCA AGGCGATGCT
    TCCGTAGATG CTATTTACAA AGCAGTCGTG GATGCAGCCA GCAAAGGGAT GCAGGTTGTC
    ATCACCACTG CTGTCAACAG TACAACTCAG ATCAGCCCCA TTCCAGCTCT GAGTGCCATG
    AGTGCCTTCA CTGCTTCCAT CGGTGACCCA TTAAGTCTCC CCAGTGCTGT CAGTGCGGTC
    ATTCACGGAC GAAACATGGG CAGTGTGGAT CACGATGGGC GGCTGAGGAG TGCGAGAGGG
    GCTCGGCTGC CTAGCAGTCT GGACCACGGG AAGAACTCCA GCGAAGGAGA TGGCTTTGAA
    TATTTCAAAT CAGCAGGTTG CCACACATCT AAAAAACAGT GGGATGGGGA GCAAAGCCCT
    GGAGGGGAGC GAAACAGGTG GAAGTGTGAG GAATTTTTAG ATCATCCAGG CCACATCCAC
    AATAGTCCTT GTCACGAAAG GCCCAACAAT GTCTCTACAC TGCCATTTCT GCCTGGGGAA
    CAGCACCCAA TACTGTTACC ACCGAGAAAC TGTCAAGGGG ATAAGATTTT GGAGGAGAAT
    TTCAGGTATA ATAACTACAA AAGAACTATG ATGAGTTTTA AGGAGAGACT AGAGAACACT
    GTGGAAAGAT GCGCACACAT TAACGGGAAC AGATCTCGGC AGAGTCGGGG CTTTGGAGAG
    CTGCTGAGCA CCACAAAGCA AGACTTTGTC CTAGAGGAGC AATCTCCGAG CTCCTCCAAT
    AGTTTGGAGA GTTCTCTGGT CAAAGACTAC ATCCATTACA ATGGAGACTT TAATGCCAAA
    AACATTAACG GGTGTGTACC TAGCCCTTCA GACGCTAAAA GCATTAGTAG TGAAGATGAC
    CTAAGGAACC CAGACTCCCC CTCTTCAAAT GAATTGATCC CTTATAGGCC AAGGACGTTC
    AACGTTGGCG ACTTGGTCTG GGGCCAAATC AAAGGACTGA CTTCCTGGCC TGGAAAATTA
    GTCAGAGAAG ACGACGTTCA CAGTTCTTGT CAACAAAGCC CCGAGGAAGG GAAGGTCTGG
    GTAATGTGGT CTGGTCTTCA TGCCTTCACT CAGGTGGAGC CCGAGAAGTT GAAGACACTA
    ACGGAAGGTT TAGAAGCCTA CAGCCGAGCC CGGAAAAGGA ACAGAAAAAG TGGAAAGCTA
    AATAACCATT TAGAAGCTGC TATTCATGAG GCCATGAGTG AACTGGACAA AATGTCTGGG
    ACTGTTCACC AAATCCCCCA GGGAGACAGA CAGATGAGAC CTCCCAAACC CAAGAGGAGG
    AAGATCTCCA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330918.1
    CDS859..6093
    Translation

    Target ORF information:

    RefSeq Version XM_015475432.2
    Organism Bos taurus(cattle)
    Definition Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475432.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGCA AGTCTGAGAT CAACCTCCAC
    CCTTTAGGTT TTCTCAACCC GAATGTAAAC GCTGCTTTAG CTTTTCTCTC CGGTGACATG
    GATGGGCAGG TATTGCAGCC TGTTCACTTT CAACTCTTAG CAGCCCTGCT TCAGAACCAA
    GCCCACGCAG CTGCCATGCT TCCCCTGCCA TCTTTCAATC TGGCCATCTC AGATATTTTG
    CAACAGCAAA ATACCCCCTT ACCCTCATTA ACACAGATGA CAGCCCCACC AGACCATTTG
    CCAAGCAATC AGTCAGAGAA CAGCCGAGCT GAGACCCTTT TAACCAACCC CCTGGGGAAC
    CCTTTACCAA GCTTTGCAGG CAGTGACACT ACTTTTAACC CCCTCTTCCT CCCAGCTGTC
    ACTGGGGCCT CAGGATTAAT GGCCTTGAAT CCCCAGCTGT TGGGAGGTGT CCTGAACTCG
    GCATCGGCCA ACACCGCTAA TCATCCAGAG GTTTCCATAG CAACTTCTTC CCAGGCAACC
    ACTACCACAA CCACTACATC ATCAGCAGTG GCAGCACTGA CTGTCTCAAC ACTTGGTGGG
    ACAGCAGTGG TGTCAATGGC AGAAACATTG CTGAATATAT CTAATAATGC TGGGAATACA
    CCTGGTCCAG CTAAACTCAA CAGTAACTCT GTGGTGCCAC AGCTACTTAA CCCTCTACTG
    GGGACAGGTC TGCTTGGTGA CATGCCCTCA ATAAACAGTA CTTTGAATAA CCATCAACTG
    ACTCATCTAC AGTCGCTGTT AAACAACAAT CAGATGTTTC CTCCAAATCA GCAGCAGCAG
    CAACTTCTTC AGGGGTACCA GAATCTCCAG GCATTTCAAG GACAGTCCAC AATTCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAATT TCCAGGTGAG AATGCAGGAA
    GATGCAGCTC TCCTAAACAA AAGAATAAGC ACTCAGCCGG GGCTCACAGC ACTTCCCGAA
    AATCCAAACA CTACACTTCC ACCTTTCCAA GACACCTCTT GTGAGTTGCA GTCGAGGATT
    GACCCATCTC TAGGTCAGCA GGTGAAGGAT GGCCTCATCA TGGGGGGCCA AGGCGATGCT
    TCCGTAGATG CTATTTACAA AGCAGTCGTG GATGCAGCCA GCAAAGGGAT GCAGGTTGTC
    ATCACCACTG CTGTCAACAG TACAACTCAG ATCAGCCCCA TTCCAGCTCT GAGTGCCATG
    AGTGCCTTCA CTGCTTCCAT CGGTGACCCA TTAAGTCTCC CCAGTGCTGT CAGTGCGGTC
    ATTCACGGAC GAAACATGGG CAGTGTGGAT CACGATGGGC GGCTGAGGAG TGCGAGAGGG
    GCTCGGCTGC CTAGCAGTCT GGACCACGGG AAGAACTCCA GCGAAGGAGA TGGCTTTGAA
    TATTTCAAAT CAGCAGGTTG CCACACATCT AAAAAACAGT GGGATGGGGA GCAAAGCCCT
    GGAGGGGAGC GAAACAGGTG GAAGTGTGAG GAATTTTTAG ATCATCCAGG CCACATCCAC
    AATAGTCCTT GTCACGAAAG GCCCAACAAT GTCTCTACAC TGCCATTTCT GCCTGGGGAA
    CAGCACCCAA TACTGTTACC ACCGAGAAAC TGTCAAGGGG ATAAGATTTT GGAGGAGAAT
    TTCAGGTATA ATAACTACAA AAGAACTATG ATGAGTTTTA AGGAGAGACT AGAGAACACT
    GTGGAAAGAT GCGCACACAT TAACGGGAAC AGATCTCGGC AGAGTCGGGG CTTTGGAGAG
    CTGCTGAGCA CCACAAAGCA AGACTTTGTC CTAGAGGAGC AATCTCCGAG CTCCTCCAAT
    AGTTTGGAGA GTTCTCTGGT CAAAGACTAC ATCCATTACA ATGGAGACTT TAATGCCAAA
    AACATTAACG GGTGTGTACC TAGCCCTTCA GACGCTAAAA GCATTAGTAG TGAAGATGAC
    CTAAGGAACC CAGACTCCCC CTCTTCAAAT GAATTGATCC CTTATAGGCC AAGGACGTTC
    AACGTTGGCG ACTTGGTCTG GGGCCAAATC AAAGGACTGA CTTCCTGGCC TGGAAAATTA
    GTCAGAGAAG ACGACGTTCA CAGTTCTTGT CAACAAAGCC CCGAGGAAGG GAAGGTCTGG
    GTAATGTGGT CTGGTCTTCA TGCCTTCACT CAGGTGGAGC CCGAGAAGTT GAAGACACTA
    ACGGAAGGTT TAGAAGCCTA CAGCCGAGCC CGGAAAAGGA ACAGAAAAAG TGGAAAGCTA
    AATAACCATT TAGAAGCTGC TATTCATGAG GCCATGAGTG AACTGGACAA AATGTCTGGG
    ACTGTTCACC AAATCCCCCA GGGAGACAGA CAGATGAGAC CTCCCAAACC CAAGAGGAGG
    AAGATCTCCA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54961
    Clone ID Related Accession (Same CDS sequence) XM_015475434.2 , XM_015475432.1 , XM_015475432.2 , XM_015475434.1
    Accession Version XM_015475434.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGCA AGTCTGAGAT CAACCTCCAC
    CCTTTAGGTT TTCTCAACCC GAATGTAAAC GCTGCTTTAG CTTTTCTCTC CGGTGACATG
    GATGGGCAGG TATTGCAGCC TGTTCACTTT CAACTCTTAG CAGCCCTGCT TCAGAACCAA
    GCCCACGCAG CTGCCATGCT TCCCCTGCCA TCTTTCAATC TGGCCATCTC AGATATTTTG
    CAACAGCAAA ATACCCCCTT ACCCTCATTA ACACAGATGA CAGCCCCACC AGACCATTTG
    CCAAGCAATC AGTCAGAGAA CAGCCGAGCT GAGACCCTTT TAACCAACCC CCTGGGGAAC
    CCTTTACCAA GCTTTGCAGG CAGTGACACT ACTTTTAACC CCCTCTTCCT CCCAGCTGTC
    ACTGGGGCCT CAGGATTAAT GGCCTTGAAT CCCCAGCTGT TGGGAGGTGT CCTGAACTCG
    GCATCGGCCA ACACCGCTAA TCATCCAGAG GTTTCCATAG CAACTTCTTC CCAGGCAACC
    ACTACCACAA CCACTACATC ATCAGCAGTG GCAGCACTGA CTGTCTCAAC ACTTGGTGGG
    ACAGCAGTGG TGTCAATGGC AGAAACATTG CTGAATATAT CTAATAATGC TGGGAATACA
    CCTGGTCCAG CTAAACTCAA CAGTAACTCT GTGGTGCCAC AGCTACTTAA CCCTCTACTG
    GGGACAGGTC TGCTTGGTGA CATGCCCTCA ATAAACAGTA CTTTGAATAA CCATCAACTG
    ACTCATCTAC AGTCGCTGTT AAACAACAAT CAGATGTTTC CTCCAAATCA GCAGCAGCAG
    CAACTTCTTC AGGGGTACCA GAATCTCCAG GCATTTCAAG GACAGTCCAC AATTCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAATT TCCAGGTGAG AATGCAGGAA
    GATGCAGCTC TCCTAAACAA AAGAATAAGC ACTCAGCCGG GGCTCACAGC ACTTCCCGAA
    AATCCAAACA CTACACTTCC ACCTTTCCAA GACACCTCTT GTGAGTTGCA GTCGAGGATT
    GACCCATCTC TAGGTCAGCA GGTGAAGGAT GGCCTCATCA TGGGGGGCCA AGGCGATGCT
    TCCGTAGATG CTATTTACAA AGCAGTCGTG GATGCAGCCA GCAAAGGGAT GCAGGTTGTC
    ATCACCACTG CTGTCAACAG TACAACTCAG ATCAGCCCCA TTCCAGCTCT GAGTGCCATG
    AGTGCCTTCA CTGCTTCCAT CGGTGACCCA TTAAGTCTCC CCAGTGCTGT CAGTGCGGTC
    ATTCACGGAC GAAACATGGG CAGTGTGGAT CACGATGGGC GGCTGAGGAG TGCGAGAGGG
    GCTCGGCTGC CTAGCAGTCT GGACCACGGG AAGAACTCCA GCGAAGGAGA TGGCTTTGAA
    TATTTCAAAT CAGCAGGTTG CCACACATCT AAAAAACAGT GGGATGGGGA GCAAAGCCCT
    GGAGGGGAGC GAAACAGGTG GAAGTGTGAG GAATTTTTAG ATCATCCAGG CCACATCCAC
    AATAGTCCTT GTCACGAAAG GCCCAACAAT GTCTCTACAC TGCCATTTCT GCCTGGGGAA
    CAGCACCCAA TACTGTTACC ACCGAGAAAC TGTCAAGGGG ATAAGATTTT GGAGGAGAAT
    TTCAGGTATA ATAACTACAA AAGAACTATG ATGAGTTTTA AGGAGAGACT AGAGAACACT
    GTGGAAAGAT GCGCACACAT TAACGGGAAC AGATCTCGGC AGAGTCGGGG CTTTGGAGAG
    CTGCTGAGCA CCACAAAGCA AGACTTTGTC CTAGAGGAGC AATCTCCGAG CTCCTCCAAT
    AGTTTGGAGA GTTCTCTGGT CAAAGACTAC ATCCATTACA ATGGAGACTT TAATGCCAAA
    AACATTAACG GGTGTGTACC TAGCCCTTCA GACGCTAAAA GCATTAGTAG TGAAGATGAC
    CTAAGGAACC CAGACTCCCC CTCTTCAAAT GAATTGATCC CTTATAGGCC AAGGACGTTC
    AACGTTGGCG ACTTGGTCTG GGGCCAAATC AAAGGACTGA CTTCCTGGCC TGGAAAATTA
    GTCAGAGAAG ACGACGTTCA CAGTTCTTGT CAACAAAGCC CCGAGGAAGG GAAGGTCTGG
    GTAATGTGGT CTGGTCTTCA TGCCTTCACT CAGGTGGAGC CCGAGAAGTT GAAGACACTA
    ACGGAAGGTT TAGAAGCCTA CAGCCGAGCC CGGAAAAGGA ACAGAAAAAG TGGAAAGCTA
    AATAACCATT TAGAAGCTGC TATTCATGAG GCCATGAGTG AACTGGACAA AATGTCTGGG
    ACTGTTCACC AAATCCCCCA GGGAGACAGA CAGATGAGAC CTCCCAAACC CAAGAGGAGG
    AAGATCTCCA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330920.1
    CDS683..5917
    Translation

    Target ORF information:

    RefSeq Version XM_015475434.1
    Organism Bos taurus(cattle)
    Definition Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475434.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGCA AGTCTGAGAT CAACCTCCAC
    CCTTTAGGTT TTCTCAACCC GAATGTAAAC GCTGCTTTAG CTTTTCTCTC CGGTGACATG
    GATGGGCAGG TATTGCAGCC TGTTCACTTT CAACTCTTAG CAGCCCTGCT TCAGAACCAA
    GCCCACGCAG CTGCCATGCT TCCCCTGCCA TCTTTCAATC TGGCCATCTC AGATATTTTG
    CAACAGCAAA ATACCCCCTT ACCCTCATTA ACACAGATGA CAGCCCCACC AGACCATTTG
    CCAAGCAATC AGTCAGAGAA CAGCCGAGCT GAGACCCTTT TAACCAACCC CCTGGGGAAC
    CCTTTACCAA GCTTTGCAGG CAGTGACACT ACTTTTAACC CCCTCTTCCT CCCAGCTGTC
    ACTGGGGCCT CAGGATTAAT GGCCTTGAAT CCCCAGCTGT TGGGAGGTGT CCTGAACTCG
    GCATCGGCCA ACACCGCTAA TCATCCAGAG GTTTCCATAG CAACTTCTTC CCAGGCAACC
    ACTACCACAA CCACTACATC ATCAGCAGTG GCAGCACTGA CTGTCTCAAC ACTTGGTGGG
    ACAGCAGTGG TGTCAATGGC AGAAACATTG CTGAATATAT CTAATAATGC TGGGAATACA
    CCTGGTCCAG CTAAACTCAA CAGTAACTCT GTGGTGCCAC AGCTACTTAA CCCTCTACTG
    GGGACAGGTC TGCTTGGTGA CATGCCCTCA ATAAACAGTA CTTTGAATAA CCATCAACTG
    ACTCATCTAC AGTCGCTGTT AAACAACAAT CAGATGTTTC CTCCAAATCA GCAGCAGCAG
    CAACTTCTTC AGGGGTACCA GAATCTCCAG GCATTTCAAG GACAGTCCAC AATTCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAATT TCCAGGTGAG AATGCAGGAA
    GATGCAGCTC TCCTAAACAA AAGAATAAGC ACTCAGCCGG GGCTCACAGC ACTTCCCGAA
    AATCCAAACA CTACACTTCC ACCTTTCCAA GACACCTCTT GTGAGTTGCA GTCGAGGATT
    GACCCATCTC TAGGTCAGCA GGTGAAGGAT GGCCTCATCA TGGGGGGCCA AGGCGATGCT
    TCCGTAGATG CTATTTACAA AGCAGTCGTG GATGCAGCCA GCAAAGGGAT GCAGGTTGTC
    ATCACCACTG CTGTCAACAG TACAACTCAG ATCAGCCCCA TTCCAGCTCT GAGTGCCATG
    AGTGCCTTCA CTGCTTCCAT CGGTGACCCA TTAAGTCTCC CCAGTGCTGT CAGTGCGGTC
    ATTCACGGAC GAAACATGGG CAGTGTGGAT CACGATGGGC GGCTGAGGAG TGCGAGAGGG
    GCTCGGCTGC CTAGCAGTCT GGACCACGGG AAGAACTCCA GCGAAGGAGA TGGCTTTGAA
    TATTTCAAAT CAGCAGGTTG CCACACATCT AAAAAACAGT GGGATGGGGA GCAAAGCCCT
    GGAGGGGAGC GAAACAGGTG GAAGTGTGAG GAATTTTTAG ATCATCCAGG CCACATCCAC
    AATAGTCCTT GTCACGAAAG GCCCAACAAT GTCTCTACAC TGCCATTTCT GCCTGGGGAA
    CAGCACCCAA TACTGTTACC ACCGAGAAAC TGTCAAGGGG ATAAGATTTT GGAGGAGAAT
    TTCAGGTATA ATAACTACAA AAGAACTATG ATGAGTTTTA AGGAGAGACT AGAGAACACT
    GTGGAAAGAT GCGCACACAT TAACGGGAAC AGATCTCGGC AGAGTCGGGG CTTTGGAGAG
    CTGCTGAGCA CCACAAAGCA AGACTTTGTC CTAGAGGAGC AATCTCCGAG CTCCTCCAAT
    AGTTTGGAGA GTTCTCTGGT CAAAGACTAC ATCCATTACA ATGGAGACTT TAATGCCAAA
    AACATTAACG GGTGTGTACC TAGCCCTTCA GACGCTAAAA GCATTAGTAG TGAAGATGAC
    CTAAGGAACC CAGACTCCCC CTCTTCAAAT GAATTGATCC CTTATAGGCC AAGGACGTTC
    AACGTTGGCG ACTTGGTCTG GGGCCAAATC AAAGGACTGA CTTCCTGGCC TGGAAAATTA
    GTCAGAGAAG ACGACGTTCA CAGTTCTTGT CAACAAAGCC CCGAGGAAGG GAAGGTCTGG
    GTAATGTGGT CTGGTCTTCA TGCCTTCACT CAGGTGGAGC CCGAGAAGTT GAAGACACTA
    ACGGAAGGTT TAGAAGCCTA CAGCCGAGCC CGGAAAAGGA ACAGAAAAAG TGGAAAGCTA
    AATAACCATT TAGAAGCTGC TATTCATGAG GCCATGAGTG AACTGGACAA AATGTCTGGG
    ACTGTTCACC AAATCCCCCA GGGAGACAGA CAGATGAGAC CTCCCAAACC CAAGAGGAGG
    AAGATCTCCA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54963
    Clone ID Related Accession (Same CDS sequence) XM_015475436.1 , XM_015475436.2
    Accession Version XM_015475436.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product methyl-CpG-binding domain protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGTG ACATGCCCTC AATAAACAGT
    ACTTTGAATA ACCATCAACT GACTCATCTA CAGTCGCTGT TAAACAACAA TCAGATGTTT
    CCTCCAAATC AGCAGCAGCA GCAACTTCTT CAGGGGTACC AGAATCTCCA GGCATTTCAA
    GGACAGTCCA CAATTCCTTG CCCAGCTAAC AATAACCCCA TGGCTTGTCT GTTTCAGAAT
    TTCCAGGTGA GAATGCAGGA AGATGCAGCT CTCCTAAACA AAAGAATAAG CACTCAGCCG
    GGGCTCACAG CACTTCCCGA AAATCCAAAC ACTACACTTC CACCTTTCCA AGACACCTCT
    TGTGAGTTGC AGTCGAGGAT TGACCCATCT CTAGGTCAGC AGGTGAAGGA TGGCCTCATC
    ATGGGGGGCC AAGGCGATGC TTCCGTAGAT GCTATTTACA AAGCAGTCGT GGATGCAGCC
    AGCAAAGGGA TGCAGGTTGT CATCACCACT GCTGTCAACA GTACAACTCA GATCAGCCCC
    ATTCCAGCTC TGAGTGCCAT GAGTGCCTTC ACTGCTTCCA TCGGTGACCC ATTAAGTCTC
    CCCAGTGCTG TCAGTGCGGT CATTCACGGA CGAAACATGG GCAGTGTGGA TCACGATGGG
    CGGCTGAGGA GTGCGAGAGG GGCTCGGCTG CCTAGCAGTC TGGACCACGG GAAGAACTCC
    AGCGAAGGAG ATGGCTTTGA ATATTTCAAA TCAGCAGGTT GCCACACATC TAAAAAACAG
    TGGGATGGGG AGCAAAGCCC TGGAGGGGAG CGAAACAGGT GGAAGTGTGA GGAATTTTTA
    GATCATCCAG GCCACATCCA CAATAGTCCT TGTCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TGCCTGGGGA ACAGCACCCA ATACTGTTAC CACCGAGAAA CTGTCAAGGG
    GATAAGATTT TGGAGGAGAA TTTCAGGTAT AATAACTACA AAAGAACTAT GATGAGTTTT
    AAGGAGAGAC TAGAGAACAC TGTGGAAAGA TGCGCACACA TTAACGGGAA CAGATCTCGG
    CAGAGTCGGG GCTTTGGAGA GCTGCTGAGC ACCACAAAGC AAGACTTTGT CCTAGAGGAG
    CAATCTCCGA GCTCCTCCAA TAGTTTGGAG AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AAACATTAAC GGGTGTGTAC CTAGCCCTTC AGACGCTAAA
    AGCATTAGTA GTGAAGATGA CCTAAGGAAC CCAGACTCCC CCTCTTCAAA TGAATTGATC
    CCTTATAGGC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGAAAATT AGTCAGAGAA GACGACGTTC ACAGTTCTTG TCAACAAAGC
    CCCGAGGAAG GGAAGGTCTG GGTAATGTGG TCTGGTCTTC ATGCCTTCAC TCAGGTGGAG
    CCCGAGAAGT TGAAGACACT AACGGAAGGT TTAGAAGCCT ACAGCCGAGC CCGGAAAAGG
    AACAGAAAAA GTGGAAAGCT AAATAACCAT TTAGAAGCTG CTATTCATGA GGCCATGAGT
    GAACTGGACA AAATGTCTGG GACTGTTCAC CAAATCCCCC AGGGAGACAG ACAGATGAGA
    CCTCCCAAAC CCAAGAGGAG GAAGATCTCC AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330922.1
    CDS857..5392
    Translation

    Target ORF information:

    RefSeq Version XM_015475436.1
    Organism Bos taurus(cattle)
    Definition Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475436.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGTG ACATGCCCTC AATAAACAGT
    ACTTTGAATA ACCATCAACT GACTCATCTA CAGTCGCTGT TAAACAACAA TCAGATGTTT
    CCTCCAAATC AGCAGCAGCA GCAACTTCTT CAGGGGTACC AGAATCTCCA GGCATTTCAA
    GGACAGTCCA CAATTCCTTG CCCAGCTAAC AATAACCCCA TGGCTTGTCT GTTTCAGAAT
    TTCCAGGTGA GAATGCAGGA AGATGCAGCT CTCCTAAACA AAAGAATAAG CACTCAGCCG
    GGGCTCACAG CACTTCCCGA AAATCCAAAC ACTACACTTC CACCTTTCCA AGACACCTCT
    TGTGAGTTGC AGTCGAGGAT TGACCCATCT CTAGGTCAGC AGGTGAAGGA TGGCCTCATC
    ATGGGGGGCC AAGGCGATGC TTCCGTAGAT GCTATTTACA AAGCAGTCGT GGATGCAGCC
    AGCAAAGGGA TGCAGGTTGT CATCACCACT GCTGTCAACA GTACAACTCA GATCAGCCCC
    ATTCCAGCTC TGAGTGCCAT GAGTGCCTTC ACTGCTTCCA TCGGTGACCC ATTAAGTCTC
    CCCAGTGCTG TCAGTGCGGT CATTCACGGA CGAAACATGG GCAGTGTGGA TCACGATGGG
    CGGCTGAGGA GTGCGAGAGG GGCTCGGCTG CCTAGCAGTC TGGACCACGG GAAGAACTCC
    AGCGAAGGAG ATGGCTTTGA ATATTTCAAA TCAGCAGGTT GCCACACATC TAAAAAACAG
    TGGGATGGGG AGCAAAGCCC TGGAGGGGAG CGAAACAGGT GGAAGTGTGA GGAATTTTTA
    GATCATCCAG GCCACATCCA CAATAGTCCT TGTCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TGCCTGGGGA ACAGCACCCA ATACTGTTAC CACCGAGAAA CTGTCAAGGG
    GATAAGATTT TGGAGGAGAA TTTCAGGTAT AATAACTACA AAAGAACTAT GATGAGTTTT
    AAGGAGAGAC TAGAGAACAC TGTGGAAAGA TGCGCACACA TTAACGGGAA CAGATCTCGG
    CAGAGTCGGG GCTTTGGAGA GCTGCTGAGC ACCACAAAGC AAGACTTTGT CCTAGAGGAG
    CAATCTCCGA GCTCCTCCAA TAGTTTGGAG AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AAACATTAAC GGGTGTGTAC CTAGCCCTTC AGACGCTAAA
    AGCATTAGTA GTGAAGATGA CCTAAGGAAC CCAGACTCCC CCTCTTCAAA TGAATTGATC
    CCTTATAGGC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGAAAATT AGTCAGAGAA GACGACGTTC ACAGTTCTTG TCAACAAAGC
    CCCGAGGAAG GGAAGGTCTG GGTAATGTGG TCTGGTCTTC ATGCCTTCAC TCAGGTGGAG
    CCCGAGAAGT TGAAGACACT AACGGAAGGT TTAGAAGCCT ACAGCCGAGC CCGGAAAAGG
    AACAGAAAAA GTGGAAAGCT AAATAACCAT TTAGAAGCTG CTATTCATGA GGCCATGAGT
    GAACTGGACA AAATGTCTGG GACTGTTCAC CAAATCCCCC AGGGAGACAG ACAGATGAGA
    CCTCCCAAAC CCAAGAGGAG GAAGATCTCC AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54963
    Clone ID Related Accession (Same CDS sequence) XM_015475436.1 , XM_015475436.2
    Accession Version XM_015475436.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product methyl-CpG-binding domain protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475436.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGTG ACATGCCCTC AATAAACAGT
    ACTTTGAATA ACCATCAACT GACTCATCTA CAGTCGCTGT TAAACAACAA TCAGATGTTT
    CCTCCAAATC AGCAGCAGCA GCAACTTCTT CAGGGGTACC AGAATCTCCA GGCATTTCAA
    GGACAGTCCA CAATTCCTTG CCCAGCTAAC AATAACCCCA TGGCTTGTCT GTTTCAGAAT
    TTCCAGGTGA GAATGCAGGA AGATGCAGCT CTCCTAAACA AAAGAATAAG CACTCAGCCG
    GGGCTCACAG CACTTCCCGA AAATCCAAAC ACTACACTTC CACCTTTCCA AGACACCTCT
    TGTGAGTTGC AGTCGAGGAT TGACCCATCT CTAGGTCAGC AGGTGAAGGA TGGCCTCATC
    ATGGGGGGCC AAGGCGATGC TTCCGTAGAT GCTATTTACA AAGCAGTCGT GGATGCAGCC
    AGCAAAGGGA TGCAGGTTGT CATCACCACT GCTGTCAACA GTACAACTCA GATCAGCCCC
    ATTCCAGCTC TGAGTGCCAT GAGTGCCTTC ACTGCTTCCA TCGGTGACCC ATTAAGTCTC
    CCCAGTGCTG TCAGTGCGGT CATTCACGGA CGAAACATGG GCAGTGTGGA TCACGATGGG
    CGGCTGAGGA GTGCGAGAGG GGCTCGGCTG CCTAGCAGTC TGGACCACGG GAAGAACTCC
    AGCGAAGGAG ATGGCTTTGA ATATTTCAAA TCAGCAGGTT GCCACACATC TAAAAAACAG
    TGGGATGGGG AGCAAAGCCC TGGAGGGGAG CGAAACAGGT GGAAGTGTGA GGAATTTTTA
    GATCATCCAG GCCACATCCA CAATAGTCCT TGTCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TGCCTGGGGA ACAGCACCCA ATACTGTTAC CACCGAGAAA CTGTCAAGGG
    GATAAGATTT TGGAGGAGAA TTTCAGGTAT AATAACTACA AAAGAACTAT GATGAGTTTT
    AAGGAGAGAC TAGAGAACAC TGTGGAAAGA TGCGCACACA TTAACGGGAA CAGATCTCGG
    CAGAGTCGGG GCTTTGGAGA GCTGCTGAGC ACCACAAAGC AAGACTTTGT CCTAGAGGAG
    CAATCTCCGA GCTCCTCCAA TAGTTTGGAG AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AAACATTAAC GGGTGTGTAC CTAGCCCTTC AGACGCTAAA
    AGCATTAGTA GTGAAGATGA CCTAAGGAAC CCAGACTCCC CCTCTTCAAA TGAATTGATC
    CCTTATAGGC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGAAAATT AGTCAGAGAA GACGACGTTC ACAGTTCTTG TCAACAAAGC
    CCCGAGGAAG GGAAGGTCTG GGTAATGTGG TCTGGTCTTC ATGCCTTCAC TCAGGTGGAG
    CCCGAGAAGT TGAAGACACT AACGGAAGGT TTAGAAGCCT ACAGCCGAGC CCGGAAAAGG
    AACAGAAAAA GTGGAAAGCT AAATAACCAT TTAGAAGCTG CTATTCATGA GGCCATGAGT
    GAACTGGACA AAATGTCTGG GACTGTTCAC CAAATCCCCC AGGGAGACAG ACAGATGAGA
    CCTCCCAAAC CCAAGAGGAG GAAGATCTCC AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330922.1
    CDS859..5394
    Translation

    Target ORF information:

    RefSeq Version XM_015475436.2
    Organism Bos taurus(cattle)
    Definition Bos taurus methyl-CpG binding domain protein 5 (MBD5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475436.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGAG GCAAGGAGTG TGACGGAGGG GACAAGGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AAAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AACCCCACAC
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC TGTAAGAAAT AAGTCACATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAACAC CATGGGAAGA
    CTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAGGAAC CCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAATGA GCATGGACAG AAGTCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CCGCGTCGTC AGGTTCCCAG ATATATGGAG ACGGCTCGAT CTCGCCAAGG
    ACTGACCCAC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ATCCTGGTTT TCACGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCA
    CCTTCTTGTG CTCTTCAGAA AAAGCCATTA ACATCTGAGA AAGATCCACT TGGCATTCTT
    GACCCTATTC CTAGTAAACC GGTGAATCAG AACCCTGTTA TCATTAATCC AACTAGTTTC
    CATTCAAATG TCCACTCTCA GGTACCTGTG ATGAATGTAA GCATGCCTCC TGCTGTTGTT
    CCTTTGCCAA GTAATCTCCC TTTGCCAACC GTAAAACCTG GTCATATGAA TCATGGGAGT
    CATGTACAAA GAGTTCAGCA TTCAGCTTCA ACCTCCCTGT CCCCTTCTCC AGTGACATCC
    CCAGTGCACA TGATGGGGAC TGGAATTGGA AGGATTGAGG CATCGCCCCA AAGATCACGC
    TCATCTTCCA CATCATCAGA TCACGGAAAT TTCATGATGC CACCTCTAGG ACCCCAGGCC
    AATTGCAGTG GTATTAAGGT TCCACCCAGG TCACCAAGGT CAACAATAGG GTCCCCAAGG
    CCATCAATGC CATCAAGCCC TTCTACCAAG TCCGATGGAC ATCATCAGTA CAAGGATATC
    CCTAACCCAT TAATTGCTGG AATGAGTAAT GTACTAAATC CCCCAAGCAG TGCAGCTTTT
    CCTACTGCAT CTGCCGGAAG TGGTTCTGTA AAGAGTCAGC CTGGTTTGCT GGGAATGCCT
    TTAAATCAGA TCTTGAACCA GCACAATGCT GCCTCCTTTC CAGCAAGTAG TTTACTCTCA
    GCAGCAGCCA AAGCACAGCT AGCAAATCAA AACAAACTTG CTGGTAACAA CAGTAGCAGC
    AGTAGCAATT CTGGAGCTGT TGCTGGCAGT GGCAACACTG AAGGACATAG CACTTTAAAC
    ACCATGTTCC CTCCTACTGC CAACATGCTT CTCCCGTCAG GTGAAGGGCA AAGTGGTCGA
    GCAGCACTAA GAGATAAGCT GATGTCTCAG CAAAAAGACT CATTGCGGAA AAGAAAACAG
    CCACCTACCA CAGTGTTGAG TTTGCTCAGA CAGTCTCAAA TGGATAGTTC TGCAGTTCCT
    AAACCTGGAC CCGACTTGCT AAGGAAGCAG GGTCAGGGGT CGTTTCCCAT CAGTTCAATG
    TCTCAGTTAC TACAGTCTAT GAGTTGTCAA AGCTCTCACT TGAGTAGCAA TAGTACCCCG
    GGTTGTGGGG GCTCAAATAC TGCTTTGCCT TGCTCTGCTA ACCAGCTGCA TTTTACAGAT
    CCCAGTATGA ACTCCAGTGT TCTTCAGAAT TCACTGACAC AGAGCATACC TTTAAGAGGG
    GAAGCCATGC ACTGCCACAA TGCAAACACT AACTTTGTTC ACAGTAATAG TCCAGTCCCA
    AACCACCATC TTACAGGTTT AATAAATCAG ATTCAGGCTA GCGGGAACTG TGGGATGCTC
    AGTCAGTCGG GCATGGCTTT AGGAAATTCA TTACATCCCA ATCCACCTCA GTCAAGAATT
    TCAACGTCCT CCACTCCAGT GATACCAAAC AGCATTGTTA GCAGCTATAA TCAAACAAGT
    TCTGAAGCAG GCGGTTCAGG ACCATCCTCC TCCATAGCCA TAGCTGGCAT CAACCACCCT
    GCCATCACAA AGACAACATC TGTTCTTCAA GACGGCGTCA TAGTCACCAC CGCAGCTGGA
    AACCCACTGC AGAGTCAGCT GCCCATTGGG AGTGATTTTC CTTTTGTTGG CCAGGAGCAC
    GCACTTCATT TTCCATCCAA CAGCACTTCA AACAACCATC TTCCACACCC CTTGAACCCC
    AGCCTCCTCA GTTCTCTACC TATCTCTTTG CCAGTGAATC AACAGCATCT CCTAAACCAG
    AATCTATTAA ATATCTTCCA GCCTTCAGCA GGAGAAGGTG ACATGCCCTC AATAAACAGT
    ACTTTGAATA ACCATCAACT GACTCATCTA CAGTCGCTGT TAAACAACAA TCAGATGTTT
    CCTCCAAATC AGCAGCAGCA GCAACTTCTT CAGGGGTACC AGAATCTCCA GGCATTTCAA
    GGACAGTCCA CAATTCCTTG CCCAGCTAAC AATAACCCCA TGGCTTGTCT GTTTCAGAAT
    TTCCAGGTGA GAATGCAGGA AGATGCAGCT CTCCTAAACA AAAGAATAAG CACTCAGCCG
    GGGCTCACAG CACTTCCCGA AAATCCAAAC ACTACACTTC CACCTTTCCA AGACACCTCT
    TGTGAGTTGC AGTCGAGGAT TGACCCATCT CTAGGTCAGC AGGTGAAGGA TGGCCTCATC
    ATGGGGGGCC AAGGCGATGC TTCCGTAGAT GCTATTTACA AAGCAGTCGT GGATGCAGCC
    AGCAAAGGGA TGCAGGTTGT CATCACCACT GCTGTCAACA GTACAACTCA GATCAGCCCC
    ATTCCAGCTC TGAGTGCCAT GAGTGCCTTC ACTGCTTCCA TCGGTGACCC ATTAAGTCTC
    CCCAGTGCTG TCAGTGCGGT CATTCACGGA CGAAACATGG GCAGTGTGGA TCACGATGGG
    CGGCTGAGGA GTGCGAGAGG GGCTCGGCTG CCTAGCAGTC TGGACCACGG GAAGAACTCC
    AGCGAAGGAG ATGGCTTTGA ATATTTCAAA TCAGCAGGTT GCCACACATC TAAAAAACAG
    TGGGATGGGG AGCAAAGCCC TGGAGGGGAG CGAAACAGGT GGAAGTGTGA GGAATTTTTA
    GATCATCCAG GCCACATCCA CAATAGTCCT TGTCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TGCCTGGGGA ACAGCACCCA ATACTGTTAC CACCGAGAAA CTGTCAAGGG
    GATAAGATTT TGGAGGAGAA TTTCAGGTAT AATAACTACA AAAGAACTAT GATGAGTTTT
    AAGGAGAGAC TAGAGAACAC TGTGGAAAGA TGCGCACACA TTAACGGGAA CAGATCTCGG
    CAGAGTCGGG GCTTTGGAGA GCTGCTGAGC ACCACAAAGC AAGACTTTGT CCTAGAGGAG
    CAATCTCCGA GCTCCTCCAA TAGTTTGGAG AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AAACATTAAC GGGTGTGTAC CTAGCCCTTC AGACGCTAAA
    AGCATTAGTA GTGAAGATGA CCTAAGGAAC CCAGACTCCC CCTCTTCAAA TGAATTGATC
    CCTTATAGGC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGAAAATT AGTCAGAGAA GACGACGTTC ACAGTTCTTG TCAACAAAGC
    CCCGAGGAAG GGAAGGTCTG GGTAATGTGG TCTGGTCTTC ATGCCTTCAC TCAGGTGGAG
    CCCGAGAAGT TGAAGACACT AACGGAAGGT TTAGAAGCCT ACAGCCGAGC CCGGAAAAGG
    AACAGAAAAA GTGGAAAGCT AAATAACCAT TTAGAAGCTG CTATTCATGA GGCCATGAGT
    GAACTGGACA AAATGTCTGG GACTGTTCAC CAAATCCCCC AGGGAGACAG ACAGATGAGA
    CCTCCCAAAC CCAAGAGGAG GAAGATCTCC AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.