TRPV4 cDNA ORF clone, Bos taurus(Cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRPV4
Entrez Gene ID	540259
Full Name	transient receptor potential cation channel subfamily V member 4
General protein information	Preferred Names transient receptor potential cation channel subfamily V member 4 Names transient receptor potential cation channel subfamily V member 4
Gene Type	protein-coding
Organism	Bos taurus(Cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001192385.2	NP_001179314.1	transient receptor potential cation channel subfamily V member 4
XM_005217902.3	XP_005217959.1	transient receptor potential cation channel subfamily V member 4 isoform X1
XM_005217903.4	XP_005217960.1	transient receptor potential cation channel subfamily V member 4 isoform X1
XM_005217902.2	XP_005217959.1	transient receptor potential cation channel subfamily V member 4 isoform X1
NM_001192385.3	NP_001179314.1	transient receptor potential cation channel subfamily V member 4
XM_005217903.3	XP_005217960.1	transient receptor potential cation channel subfamily V member 4 isoform X1
NM_001192385.1	NP_001179314.1	transient receptor potential cation channel subfamily V member 4

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRPV4	NP_067638.3
Canis lupus familiaris (dog)	TRPV4	NP_001120787.1
Bos taurus (cattle)	TRPV4	NP_001179314.1
Mus musculus (house mouse)	Trpv4	NP_071300.2
Xenopus tropicalis (tropical clawed frog)	trpv4	XP_002932129.1
Gallus gallus (chicken)	TRPV4	NP_990023.1
Pan troglodytes (chimpanzee)	TRPV4	XP_003314024.1
Rattus norvegicus (Norway rat)	Trpv4	NP_076460.1
Danio rerio (zebrafish)	trpv4	NP_001036195.1

Gene Ontology
Bibliography

Related articles in PubMed

Reduced tissue osmolarity increases TRPV4 expression and pro-inflammatory cytokines in intervertebral disc cells.
Walter BA, Purmessur D, Moon A, Occhiogrosso J, Laudier DM, Hecht AC, Iatridis JC
European cells & materials32123-36(2016 Jul)

TRPV4 channels activity in bovine articular chondrocytes: regulation by obesity-associated mediators.
S?nchez JC, L?pez-Zapata DF, Wilkins RJ
Cell calcium56(6)493-503(2014 Dec)

TRPV4 channels mediate cyclic strain-induced endothelial cell reorientation through integrin-to-integrin signaling.
Thodeti CK, Matthews B, Ravi A, Mammoto A, Ghosh K, Bracha AL, Ingber DE
Circulation research104(9)1123-30(2009 May)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

reduced tissue osmolarity, likely following proteoglycan degradation, can increase TRPV4 signalling and enhance pro-inflammatory cytokine production.
Title: Reduced tissue osmolarity increases TRPV4 expression and pro-inflammatory cytokines in intervertebral disc cells.

TRPV4 channels activity in bovine articular chondrocytes are regulated by obesity-associated mediators.
Title: TRPV4 channels activity in bovine articular chondrocytes: regulation by obesity-associated mediators.

TRPV4 channels mediate cyclic strain-induced endothelial cell reorientation through integrin-to-integrin signaling.
Title: TRPV4 channels mediate cyclic strain-induced endothelial cell reorientation through integrin-to-integrin signaling.

The following TRPV4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPV4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa27927	NM_001192385.2 Latest version!	Bos taurus transient receptor potential cation channel subfamily V member 4 (TRPV4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa38167	NM_001192385.3 Latest version!	Bos taurus transient receptor potential cation channel subfamily V member 4 (TRPV4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa27927	XM_005217902.2 Latest version!	Bos taurus transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa27927	XM_005217903.3 Latest version!	Bos taurus transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa38167	XM_005217903.4 Latest version!	Bos taurus transient receptor potential cation channel subfamily V member 4 (TRPV4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa38167	XM_005217902.3 Latest version!	Bos taurus transient receptor potential cation channel subfamily V member 4 (TRPV4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
OBa38167	NM_001192385.1	Bos taurus transient receptor potential cation channel, subfamily V, member 4 (TRPV4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRPV4 ( NM_001192385.2 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001179314.1 Bos taurus transient receptor potential cation channel subfamily V member 4 (TRPV4), mRNA.

Price & Availability↑

CloneID	OBa27927
Clone ID Related Accession (Same CDS sequence)	NM_001192385.2 , XM_005217902.2 , XM_005217903.3
Accession Version	NM_001192385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	transient receptor potential cation channel subfamily V member 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02045464.1 and DAAA02045465.1. On Jun 30, 2017 this sequence version replaced NM_001192385.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822089, SAMN02822090 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGATC CTGGCGAAGG CCCTCGTGTG GGGCCAGGGG AGATGGCCGA GTCGCCCGGG 
GATGAGAGTG GGCCCCCCGG CGGGGAGGCC TTCCCCCTGT CATCACTGGC CAACCTGTTC 
GAGGGGGAAG ACGGCTCCCC GTCGCCCTCG CCAGCTGATG GCGGTCGCTC CACCGGCCCA 
GGCGACGGGC GACCCAACCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC 
AACCCCATCG ACCTGCTGGA GTCCACCCTG TACGAGTCCT CCGTGGTGCC CGGGCCCAAG 
AAGGCGCCCA TGGACTCGCT GTTTGACTAC GGCACCTATC GTCACCACCC CAGTGACAAC 
AAGCGGTGGA GGAGGAAGGT CATAGAGAAG CAGCCACAGA GCCCCAAAGC TCCCGCACCC 
CAGCCGCCTC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG 
GGCTCCACAG CTGACCTGGA TGGGCTGCTC CCCTTCTTGC TGACCCACAA GAAGCGCCTC 
ACGGACGAGG AGTTCCGGGA ACCGTCCACC GGGAAGACCT GCCTGCCCAA GGCCCTGCTG 
AATCTGAGCA ACGGCCGCAA TGACACCATC CCTGTGCTTC TGGACATCGC CGAGCGCACG 
GGCAACATGC GGGAATTCAT CAACTCGCCC TTCCGAGACA TCTACTACCG AGGCCAGACC 
GCCCTGCACA TCGCCATCGA ACGCCGCTGC AAACACTATG TGGAGCTGCT GGTGGCCCAA 
GGAGCCGACG TCCATGCCCA GGCGCGGGGC CGCTTCTTCC AGCCCAAGGA CGAGGGCGGC 
TACTTCTACT TCGGTGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATC 
GTCAACTACC TGACGGAGAA CCCGCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGC 
GGCAACACGG TGCTGCACGC GCTGGTGGCC ATTGCCGACA ACACCCGCGA GAACACCAAG 
TTCGTCACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC 
AACCTGGAGG CCGTGCTCAA CAATGACGGC CTCTCGCCCC TCATGATGGC CGCCAAGACG 
GGCAAGATTG GGATCTTCCA ACACATCATC CGTCGGGAGG TGACAGACGA GGACACCAGG 
CACCTGTCGC GCAAGTTCAA GGACTGGGCC TATGGGCCGG TGTACTCCTC GCTCTACGAC 
CTCTCCTCCC TGGACACATG TGGGGAGGAG ACCTCTGTGC TGGAGATCCT GGTGTACAAC 
AGCAAGATCG AGAACCGCCA CGAGATGCTG GCCGTGGAAC CCATCAACGA ACTACTGCGG 
GACAAGTGGC GGAAGTTCGG GGCTGTCTCC TTCTACATCA ACGTGGTCTC CTATCTGTGT 
GCCATGGTCA TCTTTACCCT CACCGCCTAC TACCAGCCTC TCGAGGGCAC TCCGCCATAC 
CCTTACCGCA CCACAATGGA CTACCTGAGG CTGGCCGGCG AGATCATCAC ACTCTTCACT 
GGGATCCTGT TCTTTTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG 
AACTCTCTTT TCATCGACGG CTCCTTCCAG CTACTCTACT TCATCTACTC TGTGCTGGTG 
ATTGTCTCAG CGGCCCTCTA CCTGGCGGGG ATCGAGGCGT ACCTGGCCGT CATGGTCTTT 
GCCTTGGTCC TGGGCTGGAT GAACGCCCTT TACTTCACCC GCGGGCTGAA GCTGACGGGG 
ACCTATAGCA TCATGATCCA GAAGATCCTC TTCAAAGACC TTTTCCGCTT CCTGCTGGTC 
TACTTGCTCT TCATGATCGG CTACGCGTCA GCCCTGGTCT CGCTCCTGAA CCCGTGTGCC 
AACTTGAAGG TGTGCGATGA GGACCACGCC AACTGCACAG TGCCCACCTA CCCCTCATGC 
CGCGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTCT TCAAGCTCAC CATCGGCATG 
GGTGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTC 
ACGTACATCA TCCTCACATT CGTGCTGCTC CTTAACATGC TCATCGCCCT CATGGGGGAG 
ACAGTGGGCC AGGTGTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC 
ATCCTGGACA TCGAGCGCTC CTTCCCCGTG TTCCTGAGGA AGGCCTTCCG CTCCGGTGAA 
ATGGTGACCG TGGGCAAGAG CTCGGACGGC ACTCCAGACC GCAGGTGGTG CTTCAGGGTG 
GACGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATTAACGA GGACCCCGGC 
AAGAACGAGA ACTACCAGTA CTATGGCTTC TCGCACACCG TGGGCCGCCT CCGGAGGGAT 
CGCTGGTCCT CAGTGGTGCC CCGCGTGGTA GAGCTCAACA AGAACTCCAA CCCGGACGAG 
GTGGTGGTGC CTCTGGACAA CGTAGGGAAC CCCAGCTGCG ACGGCCACCA GCAGAGTTAC 
CCCCCTAAGT GGAGGACTGA CGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179314.1
CDS	1..2616
Translation	MADPGEGPRVGPGEMAESPGDESGPPGGEAFPLSSLANLFEGEDGSPSPSPADGGRSTGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHPSDNKRWRRKVIEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTIPVLLDIAERTGNMREFINSPFRDIYYRGQTALHIAIERRCKHYVELLVAQGADVHAQARGRFFQPKDEGGYFYFGELPLSLAACTNQPHIVNYLTENPHKKADMRRQDSRGNTVLHALVAIADNTRENTKFVTKMYDLLLLKCARLFPDSNLEAVLNNDGLSPLMMAAKTGKIGIFQHIIRREVTDEDTRHLSRKFKDWAYGPVYSSLYDLSSLDTCGEETSVLEILVYNSKIENRHEMLAVEPINELLRDKWRKFGAVSFYINVVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTMDYLRLAGEIITLFTGILFFFTNIKDLFMKKCPGVNSLFIDGSFQLLYFIYSVLVIVSAALYLAGIEAYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVSLLNPCANLKVCDEDHANCTVPTYPSCRDSETFSTFLLDLFKLTIGMGDLEMLSSTKYPVVFIILLVTYIILTFVLLLNMLIALMGETVGQVSKESKHIWKLQWATTILDIERSFPVFLRKAFRSGEMVTVGKSSDGTPDRRWCFRVDEVNWSHWNQNLGIINEDPGKNENYQYYGFSHTVGRLRRDRWSSVVPRVVELNKNSNPDEVVVPLDNVGNPSCDGHQQSYPPKWRTDDAPL

Target ORF information:

RefSeq Version	NM_001192385.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transient receptor potential cation channel subfamily V member 4 (TRPV4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192385.2

ORF Insert Sequence:

ATGGCGGATC CTGGCGAAGG CCCTCGTGTG GGGCCAGGGG AGATGGCCGA GTCGCCCGGG 
GATGAGAGTG GGCCCCCCGG CGGGGAGGCC TTCCCCCTGT CATCACTGGC CAACCTGTTC 
GAGGGGGAAG ACGGCTCCCC GTCGCCCTCG CCAGCTGATG GCGGTCGCTC CACCGGCCCA 
GGCGACGGGC GACCCAACCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC 
AACCCCATCG ACCTGCTGGA GTCCACCCTG TACGAGTCCT CCGTGGTGCC CGGGCCCAAG 
AAGGCGCCCA TGGACTCGCT GTTTGACTAC GGCACCTATC GTCACCACCC CAGTGACAAC 
AAGCGGTGGA GGAGGAAGGT CATAGAGAAG CAGCCACAGA GCCCCAAAGC TCCCGCACCC 
CAGCCGCCTC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG 
GGCTCCACAG CTGACCTGGA TGGGCTGCTC CCCTTCTTGC TGACCCACAA GAAGCGCCTC 
ACGGACGAGG AGTTCCGGGA ACCGTCCACC GGGAAGACCT GCCTGCCCAA GGCCCTGCTG 
AATCTGAGCA ACGGCCGCAA TGACACCATC CCTGTGCTTC TGGACATCGC CGAGCGCACG 
GGCAACATGC GGGAATTCAT CAACTCGCCC TTCCGAGACA TCTACTACCG AGGCCAGACC 
GCCCTGCACA TCGCCATCGA ACGCCGCTGC AAACACTATG TGGAGCTGCT GGTGGCCCAA 
GGAGCCGACG TCCATGCCCA GGCGCGGGGC CGCTTCTTCC AGCCCAAGGA CGAGGGCGGC 
TACTTCTACT TCGGTGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATC 
GTCAACTACC TGACGGAGAA CCCGCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGC 
GGCAACACGG TGCTGCACGC GCTGGTGGCC ATTGCCGACA ACACCCGCGA GAACACCAAG 
TTCGTCACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC 
AACCTGGAGG CCGTGCTCAA CAATGACGGC CTCTCGCCCC TCATGATGGC CGCCAAGACG 
GGCAAGATTG GGATCTTCCA ACACATCATC CGTCGGGAGG TGACAGACGA GGACACCAGG 
CACCTGTCGC GCAAGTTCAA GGACTGGGCC TATGGGCCGG TGTACTCCTC GCTCTACGAC 
CTCTCCTCCC TGGACACATG TGGGGAGGAG ACCTCTGTGC TGGAGATCCT GGTGTACAAC 
AGCAAGATCG AGAACCGCCA CGAGATGCTG GCCGTGGAAC CCATCAACGA ACTACTGCGG 
GACAAGTGGC GGAAGTTCGG GGCTGTCTCC TTCTACATCA ACGTGGTCTC CTATCTGTGT 
GCCATGGTCA TCTTTACCCT CACCGCCTAC TACCAGCCTC TCGAGGGCAC TCCGCCATAC 
CCTTACCGCA CCACAATGGA CTACCTGAGG CTGGCCGGCG AGATCATCAC ACTCTTCACT 
GGGATCCTGT TCTTTTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG 
AACTCTCTTT TCATCGACGG CTCCTTCCAG CTACTCTACT TCATCTACTC TGTGCTGGTG 
ATTGTCTCAG CGGCCCTCTA CCTGGCGGGG ATCGAGGCGT ACCTGGCCGT CATGGTCTTT 
GCCTTGGTCC TGGGCTGGAT GAACGCCCTT TACTTCACCC GCGGGCTGAA GCTGACGGGG 
ACCTATAGCA TCATGATCCA GAAGATCCTC TTCAAAGACC TTTTCCGCTT CCTGCTGGTC 
TACTTGCTCT TCATGATCGG CTACGCGTCA GCCCTGGTCT CGCTCCTGAA CCCGTGTGCC 
AACTTGAAGG TGTGCGATGA GGACCACGCC AACTGCACAG TGCCCACCTA CCCCTCATGC 
CGCGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTCT TCAAGCTCAC CATCGGCATG 
GGTGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTC 
ACGTACATCA TCCTCACATT CGTGCTGCTC CTTAACATGC TCATCGCCCT CATGGGGGAG 
ACAGTGGGCC AGGTGTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC 
ATCCTGGACA TCGAGCGCTC CTTCCCCGTG TTCCTGAGGA AGGCCTTCCG CTCCGGTGAA 
ATGGTGACCG TGGGCAAGAG CTCGGACGGC ACTCCAGACC GCAGGTGGTG CTTCAGGGTG 
GACGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATTAACGA GGACCCCGGC 
AAGAACGAGA ACTACCAGTA CTATGGCTTC TCGCACACCG TGGGCCGCCT CCGGAGGGAT 
CGCTGGTCCT CAGTGGTGCC CCGCGTGGTA GAGCTCAACA AGAACTCCAA CCCGGACGAG 
GTGGTGGTGC CTCTGGACAA CGTAGGGAAC CCCAGCTGCG ACGGCCACCA GCAGAGTTAC 
CCCCCTAAGT GGAGGACTGA CGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPV4 ( NM_001192385.3 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001179314.1 Bos taurus transient receptor potential cation channel subfamily V member 4 (TRPV4), mRNA.

Price & Availability↑

CloneID	OBa38167
Clone ID Related Accession (Same CDS sequence)	XM_005217902.3 , XM_005217903.4 , NM_001192385.3 , NM_001192385.1
Accession Version	NM_001192385.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	transient receptor potential cation channel subfamily V member 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000017.1. On May 29, 2018 this sequence version replaced NM_001192385.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGATC CTGGCGAAGG CCCTCGTGTG GGGCCAGGGG AGATGGCCGA GTCGCCCGGG 
GATGAGAGTG GGCCCCCCGG CGGGGAGGCC TTCCCCCTGT CATCACTGGC CAACCTGTTC 
GAGGGGGAAG ACGGCTCCCC GTCGCCCTCG CCAGCTGATG GCGGTCGCTC CACCGGCCCA 
GGCGACGGGC GACCCAACCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC 
AACCCCATCG ACCTGCTGGA GTCCACCCTG TACGAGTCCT CCGTGGTGCC CGGGCCCAAG 
AAGGCGCCCA TGGACTCGCT GTTTGACTAC GGCACCTATC GTCACCACCC CAGTGACAAC 
AAGCGGTGGA GGAGGAAGGT CATAGAGAAG CAGCCACAGA GCCCCAAAGC TCCCGCACCC 
CAGCCGCCTC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG 
GGCTCCACAG CTGACCTGGA TGGGCTGCTC CCCTTCTTGC TGACCCACAA GAAGCGCCTC 
ACGGACGAGG AGTTCCGGGA ACCGTCCACC GGGAAGACCT GCCTGCCCAA GGCCCTGCTG 
AATCTGAGCA ACGGCCGCAA TGACACCATC CCTGTGCTTC TGGACATCGC CGAGCGCACG 
GGCAACATGC GGGAATTCAT CAACTCGCCC TTCCGAGACA TCTACTACCG AGGCCAGACC 
GCCCTGCACA TCGCCATCGA ACGCCGCTGC AAACACTATG TGGAGCTGCT GGTGGCCCAA 
GGAGCCGACG TCCATGCCCA GGCGCGGGGC CGCTTCTTCC AGCCCAAGGA CGAGGGCGGC 
TACTTCTACT TCGGTGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATC 
GTCAACTACC TGACGGAGAA CCCGCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGC 
GGCAACACGG TGCTGCACGC ACTGGTGGCC ATTGCCGACA ACACCCGCGA GAACACCAAG 
TTCGTCACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC 
AACCTGGAGG CCGTGCTCAA CAATGACGGC CTCTCGCCCC TCATGATGGC CGCCAAGACG 
GGCAAGATTG GGATCTTCCA ACACATCATC CGTCGGGAGG TGACAGACGA GGACACCAGG 
CACCTGTCGC GCAAGTTCAA GGACTGGGCC TATGGGCCGG TGTACTCCTC GCTCTACGAC 
CTCTCCTCCC TGGACACATG TGGGGAGGAG ACCTCTGTGC TGGAGATCCT GGTGTACAAC 
AGCAAGATCG AGAACCGCCA CGAGATGCTG GCCGTGGAAC CCATCAACGA ACTACTGCGG 
GACAAGTGGC GGAAGTTCGG GGCTGTCTCC TTCTACATCA ACGTGGTCTC CTATCTGTGT 
GCCATGGTCA TCTTTACCCT CACCGCCTAC TACCAGCCTC TCGAGGGCAC TCCGCCATAC 
CCTTACCGCA CCACAATGGA CTACCTGAGG CTGGCCGGCG AGATCATCAC ACTCTTCACT 
GGGATCCTGT TCTTTTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG 
AACTCTCTTT TCATCGACGG CTCCTTCCAG CTACTCTACT TCATCTACTC TGTGCTGGTG 
ATTGTCTCAG CGGCCCTCTA CCTGGCGGGG ATCGAGGCGT ACCTGGCCGT CATGGTCTTT 
GCCTTGGTCC TGGGCTGGAT GAACGCCCTT TACTTCACCC GCGGGCTGAA GCTGACGGGG 
ACCTATAGCA TCATGATCCA GAAGATCCTC TTCAAAGACC TTTTCCGCTT CCTGCTGGTC 
TACTTGCTCT TCATGATCGG CTACGCGTCA GCCCTGGTCT CGCTCCTGAA CCCGTGTGCC 
AACTTGAAGG TGTGCGATGA GGACCACGCC AACTGCACAG TGCCCACCTA CCCCTCATGC 
CGCGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTCT TCAAGCTCAC CATCGGCATG 
GGTGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTC 
ACGTACATCA TCCTCACATT CGTGCTGCTC CTTAACATGC TCATCGCCCT CATGGGGGAG 
ACAGTGGGCC AGGTGTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC 
ATCCTGGACA TCGAGCGCTC CTTCCCCGTG TTCCTGAGGA AGGCCTTCCG CTCCGGTGAA 
ATGGTGACCG TGGGCAAGAG CTCGGACGGC ACTCCAGACC GCAGGTGGTG CTTCAGGGTG 
GACGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATTAACGA GGACCCCGGC 
AAGAACGAGA ACTACCAGTA CTATGGCTTC TCGCACACCG TGGGCCGCCT CCGGAGGGAT 
CGCTGGTCCT CAGTGGTGCC CCGCGTGGTA GAGCTCAACA AGAACTCCAA CCCGGACGAG 
GTGGTGGTGC CTCTGGACAA CGTAGGGAAC CCCAGCTGCG ACGGCCACCA GCAGAGTTAC 
CCCCCTAAGT GGAGGACTGA CGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179314.1
CDS	1..2616
Translation	MADPGEGPRVGPGEMAESPGDESGPPGGEAFPLSSLANLFEGEDGSPSPSPADGGRSTGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHPSDNKRWRRKVIEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTIPVLLDIAERTGNMREFINSPFRDIYYRGQTALHIAIERRCKHYVELLVAQGADVHAQARGRFFQPKDEGGYFYFGELPLSLAACTNQPHIVNYLTENPHKKADMRRQDSRGNTVLHALVAIADNTRENTKFVTKMYDLLLLKCARLFPDSNLEAVLNNDGLSPLMMAAKTGKIGIFQHIIRREVTDEDTRHLSRKFKDWAYGPVYSSLYDLSSLDTCGEETSVLEILVYNSKIENRHEMLAVEPINELLRDKWRKFGAVSFYINVVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTMDYLRLAGEIITLFTGILFFFTNIKDLFMKKCPGVNSLFIDGSFQLLYFIYSVLVIVSAALYLAGIEAYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVSLLNPCANLKVCDEDHANCTVPTYPSCRDSETFSTFLLDLFKLTIGMGDLEMLSSTKYPVVFIILLVTYIILTFVLLLNMLIALMGETVGQVSKESKHIWKLQWATTILDIERSFPVFLRKAFRSGEMVTVGKSSDGTPDRRWCFRVDEVNWSHWNQNLGIINEDPGKNENYQYYGFSHTVGRLRRDRWSSVVPRVVELNKNSNPDEVVVPLDNVGNPSCDGHQQSYPPKWRTDDAPL

Target ORF information:

RefSeq Version	NM_001192385.3
Organism	Bos taurus(cattle)
Definition	Bos taurus transient receptor potential cation channel subfamily V member 4 (TRPV4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192385.3

ORF Insert Sequence:

ATGGCGGATC CTGGCGAAGG CCCTCGTGTG GGGCCAGGGG AGATGGCCGA GTCGCCCGGG 
GATGAGAGTG GGCCCCCCGG CGGGGAGGCC TTCCCCCTGT CATCACTGGC CAACCTGTTC 
GAGGGGGAAG ACGGCTCCCC GTCGCCCTCG CCAGCTGATG GCGGTCGCTC CACCGGCCCA 
GGCGACGGGC GACCCAACCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC 
AACCCCATCG ACCTGCTGGA GTCCACCCTG TACGAGTCCT CCGTGGTGCC CGGGCCCAAG 
AAGGCGCCCA TGGACTCGCT GTTTGACTAC GGCACCTATC GTCACCACCC CAGTGACAAC 
AAGCGGTGGA GGAGGAAGGT CATAGAGAAG CAGCCACAGA GCCCCAAAGC TCCCGCACCC 
CAGCCGCCTC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG 
GGCTCCACAG CTGACCTGGA TGGGCTGCTC CCCTTCTTGC TGACCCACAA GAAGCGCCTC 
ACGGACGAGG AGTTCCGGGA ACCGTCCACC GGGAAGACCT GCCTGCCCAA GGCCCTGCTG 
AATCTGAGCA ACGGCCGCAA TGACACCATC CCTGTGCTTC TGGACATCGC CGAGCGCACG 
GGCAACATGC GGGAATTCAT CAACTCGCCC TTCCGAGACA TCTACTACCG AGGCCAGACC 
GCCCTGCACA TCGCCATCGA ACGCCGCTGC AAACACTATG TGGAGCTGCT GGTGGCCCAA 
GGAGCCGACG TCCATGCCCA GGCGCGGGGC CGCTTCTTCC AGCCCAAGGA CGAGGGCGGC 
TACTTCTACT TCGGTGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATC 
GTCAACTACC TGACGGAGAA CCCGCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGC 
GGCAACACGG TGCTGCACGC ACTGGTGGCC ATTGCCGACA ACACCCGCGA GAACACCAAG 
TTCGTCACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC 
AACCTGGAGG CCGTGCTCAA CAATGACGGC CTCTCGCCCC TCATGATGGC CGCCAAGACG 
GGCAAGATTG GGATCTTCCA ACACATCATC CGTCGGGAGG TGACAGACGA GGACACCAGG 
CACCTGTCGC GCAAGTTCAA GGACTGGGCC TATGGGCCGG TGTACTCCTC GCTCTACGAC 
CTCTCCTCCC TGGACACATG TGGGGAGGAG ACCTCTGTGC TGGAGATCCT GGTGTACAAC 
AGCAAGATCG AGAACCGCCA CGAGATGCTG GCCGTGGAAC CCATCAACGA ACTACTGCGG 
GACAAGTGGC GGAAGTTCGG GGCTGTCTCC TTCTACATCA ACGTGGTCTC CTATCTGTGT 
GCCATGGTCA TCTTTACCCT CACCGCCTAC TACCAGCCTC TCGAGGGCAC TCCGCCATAC 
CCTTACCGCA CCACAATGGA CTACCTGAGG CTGGCCGGCG AGATCATCAC ACTCTTCACT 
GGGATCCTGT TCTTTTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG 
AACTCTCTTT TCATCGACGG CTCCTTCCAG CTACTCTACT TCATCTACTC TGTGCTGGTG 
ATTGTCTCAG CGGCCCTCTA CCTGGCGGGG ATCGAGGCGT ACCTGGCCGT CATGGTCTTT 
GCCTTGGTCC TGGGCTGGAT GAACGCCCTT TACTTCACCC GCGGGCTGAA GCTGACGGGG 
ACCTATAGCA TCATGATCCA GAAGATCCTC TTCAAAGACC TTTTCCGCTT CCTGCTGGTC 
TACTTGCTCT TCATGATCGG CTACGCGTCA GCCCTGGTCT CGCTCCTGAA CCCGTGTGCC 
AACTTGAAGG TGTGCGATGA GGACCACGCC AACTGCACAG TGCCCACCTA CCCCTCATGC 
CGCGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTCT TCAAGCTCAC CATCGGCATG 
GGTGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTC 
ACGTACATCA TCCTCACATT CGTGCTGCTC CTTAACATGC TCATCGCCCT CATGGGGGAG 
ACAGTGGGCC AGGTGTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC 
ATCCTGGACA TCGAGCGCTC CTTCCCCGTG TTCCTGAGGA AGGCCTTCCG CTCCGGTGAA 
ATGGTGACCG TGGGCAAGAG CTCGGACGGC ACTCCAGACC GCAGGTGGTG CTTCAGGGTG 
GACGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATTAACGA GGACCCCGGC 
AAGAACGAGA ACTACCAGTA CTATGGCTTC TCGCACACCG TGGGCCGCCT CCGGAGGGAT 
CGCTGGTCCT CAGTGGTGCC CCGCGTGGTA GAGCTCAACA AGAACTCCAA CCCGGACGAG 
GTGGTGGTGC CTCTGGACAA CGTAGGGAAC CCCAGCTGCG ACGGCCACCA GCAGAGTTAC 
CCCCCTAAGT GGAGGACTGA CGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPV4 ( NM_001192385.1 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001179314.1 Bos taurus transient receptor potential cation channel, subfamily V, member 4 (TRPV4), mRNA.

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CloneID	OBa38167
Clone ID Related Accession (Same CDS sequence)	XM_005217902.3 , XM_005217903.4 , NM_001192385.3 , NM_001192385.1
Accession Version	NM_001192385.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-23
Organism	Bos taurus(Cattle)
Product	transient receptor potential cation channel subfamily V member 4
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03044400.1 and AAFC03044399.1. On Jul 17, 2010 this sequence version replaced XM_592740.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02404551, SAMN02404559 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGATC CTGGCGAAGG CCCTCGTGTG GGGCCAGGGG AGATGGCCGA GTCGCCCGGG 
GATGAGAGTG GGCCCCCCGG CGGGGAGGCC TTCCCCCTGT CATCACTGGC CAACCTGTTC 
GAGGGGGAAG ACGGCTCCCC GTCGCCCTCG CCAGCTGATG GCGGTCGCTC CACCGGCCCA 
GGCGACGGGC GACCCAACCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC 
AACCCCATCG ACCTGCTGGA GTCCACCCTG TACGAGTCCT CCGTGGTGCC CGGGCCCAAG 
AAGGCGCCCA TGGACTCGCT GTTTGACTAC GGCACCTATC GTCACCACCC CAGTGACAAC 
AAGCGGTGGA GGAGGAAGGT CATAGAGAAG CAGCCACAGA GCCCCAAAGC TCCCGCACCC 
CAGCCGCCTC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG 
GGCTCCACAG CTGACCTGGA TGGGCTGCTC CCCTTCTTGC TGACCCACAA GAAGCGCCTC 
ACGGACGAGG AGTTCCGGGA ACCGTCCACC GGGAAGACCT GCCTGCCCAA GGCCCTGCTG 
AATCTGAGCA ACGGCCGCAA TGACACCATC CCTGTGCTTC TGGACATCGC CGAGCGCACG 
GGCAACATGC GGGAATTCAT CAACTCGCCC TTCCGAGACA TCTACTACCG AGGCCAGACC 
GCCCTGCACA TCGCCATCGA ACGCCGCTGC AAACACTATG TGGAGCTGCT GGTGGCCCAA 
GGAGCCGACG TCCATGCCCA GGCGCGGGGC CGCTTCTTCC AGCCCAAGGA CGAGGGCGGC 
TACTTCTACT TCGGTGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATC 
GTCAACTACC TGACGGAGAA CCCGCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGC 
GGCAACACGG TGCTGCACGC ACTGGTGGCC ATTGCCGACA ACACCCGCGA GAACACCAAG 
TTCGTCACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC 
AACCTGGAGG CCGTGCTCAA CAATGACGGC CTCTCGCCCC TCATGATGGC CGCCAAGACG 
GGCAAGATTG GGATCTTCCA ACACATCATC CGTCGGGAGG TGACAGACGA GGACACCAGG 
CACCTGTCGC GCAAGTTCAA GGACTGGGCC TATGGGCCGG TGTACTCCTC GCTCTACGAC 
CTCTCCTCCC TGGACACATG TGGGGAGGAG ACCTCTGTGC TGGAGATCCT GGTGTACAAC 
AGCAAGATCG AGAACCGCCA CGAGATGCTG GCCGTGGAAC CCATCAACGA ACTACTGCGG 
GACAAGTGGC GGAAGTTCGG GGCTGTCTCC TTCTACATCA ACGTGGTCTC CTATCTGTGT 
GCCATGGTCA TCTTTACCCT CACCGCCTAC TACCAGCCTC TCGAGGGCAC TCCGCCATAC 
CCTTACCGCA CCACAATGGA CTACCTGAGG CTGGCCGGCG AGATCATCAC ACTCTTCACT 
GGGATCCTGT TCTTTTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG 
AACTCTCTTT TCATCGACGG CTCCTTCCAG CTACTCTACT TCATCTACTC TGTGCTGGTG 
ATTGTCTCAG CGGCCCTCTA CCTGGCGGGG ATCGAGGCGT ACCTGGCCGT CATGGTCTTT 
GCCTTGGTCC TGGGCTGGAT GAACGCCCTT TACTTCACCC GCGGGCTGAA GCTGACGGGG 
ACCTATAGCA TCATGATCCA GAAGATCCTC TTCAAAGACC TTTTCCGCTT CCTGCTGGTC 
TACTTGCTCT TCATGATCGG CTACGCGTCA GCCCTGGTCT CGCTCCTGAA CCCGTGTGCC 
AACTTGAAGG TGTGCGATGA GGACCACGCC AACTGCACAG TGCCCACCTA CCCCTCATGC 
CGCGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTCT TCAAGCTCAC CATCGGCATG 
GGTGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTC 
ACGTACATCA TCCTCACATT CGTGCTGCTC CTTAACATGC TCATCGCCCT CATGGGGGAG 
ACAGTGGGCC AGGTGTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC 
ATCCTGGACA TCGAGCGCTC CTTCCCCGTG TTCCTGAGGA AGGCCTTCCG CTCCGGTGAA 
ATGGTGACCG TGGGCAAGAG CTCGGACGGC ACTCCAGACC GCAGGTGGTG CTTCAGGGTG 
GACGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATTAACGA GGACCCCGGC 
AAGAACGAGA ACTACCAGTA CTATGGCTTC TCGCACACCG TGGGCCGCCT CCGGAGGGAT 
CGCTGGTCCT CAGTGGTGCC CCGCGTGGTA GAGCTCAACA AGAACTCCAA CCCGGACGAG 
GTGGTGGTGC CTCTGGACAA CGTAGGGAAC CCCAGCTGCG ACGGCCACCA GCAGAGTTAC 
CCCCCTAAGT GGAGGACTGA CGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179314.1
CDS	1..2616
Translation	MADPGEGPRVGPGEMAESPGDESGPPGGEAFPLSSLANLFEGEDGSPSPSPADGGRSTGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHPSDNKRWRRKVIEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTIPVLLDIAERTGNMREFINSPFRDIYYRGQTALHIAIERRCKHYVELLVAQGADVHAQARGRFFQPKDEGGYFYFGELPLSLAACTNQPHIVNYLTENPHKKADMRRQDSRGNTVLHALVAIADNTRENTKFVTKMYDLLLLKCARLFPDSNLEAVLNNDGLSPLMMAAKTGKIGIFQHIIRREVTDEDTRHLSRKFKDWAYGPVYSSLYDLSSLDTCGEETSVLEILVYNSKIENRHEMLAVEPINELLRDKWRKFGAVSFYINVVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTMDYLRLAGEIITLFTGILFFFTNIKDLFMKKCPGVNSLFIDGSFQLLYFIYSVLVIVSAALYLAGIEAYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVSLLNPCANLKVCDEDHANCTVPTYPSCRDSETFSTFLLDLFKLTIGMGDLEMLSSTKYPVVFIILLVTYIILTFVLLLNMLIALMGETVGQVSKESKHIWKLQWATTILDIERSFPVFLRKAFRSGEMVTVGKSSDGTPDRRWCFRVDEVNWSHWNQNLGIINEDPGKNENYQYYGFSHTVGRLRRDRWSSVVPRVVELNKNSNPDEVVVPLDNVGNPSCDGHQQSYPPKWRTDDAPL

Target ORF information:

RefSeq Version	NM_001192385.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus transient receptor potential cation channel, subfamily V, member 4 (TRPV4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192385.1

ORF Insert Sequence:

ATGGCGGATC CTGGCGAAGG CCCTCGTGTG GGGCCAGGGG AGATGGCCGA GTCGCCCGGG 
GATGAGAGTG GGCCCCCCGG CGGGGAGGCC TTCCCCCTGT CATCACTGGC CAACCTGTTC 
GAGGGGGAAG ACGGCTCCCC GTCGCCCTCG CCAGCTGATG GCGGTCGCTC CACCGGCCCA 
GGCGACGGGC GACCCAACCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC 
AACCCCATCG ACCTGCTGGA GTCCACCCTG TACGAGTCCT CCGTGGTGCC CGGGCCCAAG 
AAGGCGCCCA TGGACTCGCT GTTTGACTAC GGCACCTATC GTCACCACCC CAGTGACAAC 
AAGCGGTGGA GGAGGAAGGT CATAGAGAAG CAGCCACAGA GCCCCAAAGC TCCCGCACCC 
CAGCCGCCTC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG 
GGCTCCACAG CTGACCTGGA TGGGCTGCTC CCCTTCTTGC TGACCCACAA GAAGCGCCTC 
ACGGACGAGG AGTTCCGGGA ACCGTCCACC GGGAAGACCT GCCTGCCCAA GGCCCTGCTG 
AATCTGAGCA ACGGCCGCAA TGACACCATC CCTGTGCTTC TGGACATCGC CGAGCGCACG 
GGCAACATGC GGGAATTCAT CAACTCGCCC TTCCGAGACA TCTACTACCG AGGCCAGACC 
GCCCTGCACA TCGCCATCGA ACGCCGCTGC AAACACTATG TGGAGCTGCT GGTGGCCCAA 
GGAGCCGACG TCCATGCCCA GGCGCGGGGC CGCTTCTTCC AGCCCAAGGA CGAGGGCGGC 
TACTTCTACT TCGGTGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATC 
GTCAACTACC TGACGGAGAA CCCGCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGC 
GGCAACACGG TGCTGCACGC ACTGGTGGCC ATTGCCGACA ACACCCGCGA GAACACCAAG 
TTCGTCACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC 
AACCTGGAGG CCGTGCTCAA CAATGACGGC CTCTCGCCCC TCATGATGGC CGCCAAGACG 
GGCAAGATTG GGATCTTCCA ACACATCATC CGTCGGGAGG TGACAGACGA GGACACCAGG 
CACCTGTCGC GCAAGTTCAA GGACTGGGCC TATGGGCCGG TGTACTCCTC GCTCTACGAC 
CTCTCCTCCC TGGACACATG TGGGGAGGAG ACCTCTGTGC TGGAGATCCT GGTGTACAAC 
AGCAAGATCG AGAACCGCCA CGAGATGCTG GCCGTGGAAC CCATCAACGA ACTACTGCGG 
GACAAGTGGC GGAAGTTCGG GGCTGTCTCC TTCTACATCA ACGTGGTCTC CTATCTGTGT 
GCCATGGTCA TCTTTACCCT CACCGCCTAC TACCAGCCTC TCGAGGGCAC TCCGCCATAC 
CCTTACCGCA CCACAATGGA CTACCTGAGG CTGGCCGGCG AGATCATCAC ACTCTTCACT 
GGGATCCTGT TCTTTTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG 
AACTCTCTTT TCATCGACGG CTCCTTCCAG CTACTCTACT TCATCTACTC TGTGCTGGTG 
ATTGTCTCAG CGGCCCTCTA CCTGGCGGGG ATCGAGGCGT ACCTGGCCGT CATGGTCTTT 
GCCTTGGTCC TGGGCTGGAT GAACGCCCTT TACTTCACCC GCGGGCTGAA GCTGACGGGG 
ACCTATAGCA TCATGATCCA GAAGATCCTC TTCAAAGACC TTTTCCGCTT CCTGCTGGTC 
TACTTGCTCT TCATGATCGG CTACGCGTCA GCCCTGGTCT CGCTCCTGAA CCCGTGTGCC 
AACTTGAAGG TGTGCGATGA GGACCACGCC AACTGCACAG TGCCCACCTA CCCCTCATGC 
CGCGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTCT TCAAGCTCAC CATCGGCATG 
GGTGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTC 
ACGTACATCA TCCTCACATT CGTGCTGCTC CTTAACATGC TCATCGCCCT CATGGGGGAG 
ACAGTGGGCC AGGTGTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC 
ATCCTGGACA TCGAGCGCTC CTTCCCCGTG TTCCTGAGGA AGGCCTTCCG CTCCGGTGAA 
ATGGTGACCG TGGGCAAGAG CTCGGACGGC ACTCCAGACC GCAGGTGGTG CTTCAGGGTG 
GACGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATTAACGA GGACCCCGGC 
AAGAACGAGA ACTACCAGTA CTATGGCTTC TCGCACACCG TGGGCCGCCT CCGGAGGGAT 
CGCTGGTCCT CAGTGGTGCC CCGCGTGGTA GAGCTCAACA AGAACTCCAA CCCGGACGAG 
GTGGTGGTGC CTCTGGACAA CGTAGGGAAC CCCAGCTGCG ACGGCCACCA GCAGAGTTAC 
CCCCCTAAGT GGAGGACTGA CGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPV4 ( XM_005217902.2 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_005217959.1 Bos taurus transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X1, mRNA.

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CloneID	OBa27927
Clone ID Related Accession (Same CDS sequence)	NM_001192385.2 , XM_005217902.2 , XM_005217903.3
Accession Version	XM_005217902.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transient receptor potential cation channel subfamily V member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005217902.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CTGGCGAAGG CCCTCGTGTG GGGCCAGGGG AGATGGCCGA GTCGCCCGGG 
GATGAGAGTG GGCCCCCCGG CGGGGAGGCC TTCCCCCTGT CATCACTGGC CAACCTGTTC 
GAGGGGGAAG ACGGCTCCCC GTCGCCCTCG CCAGCTGATG GCGGTCGCTC CACCGGCCCA 
GGCGACGGGC GACCCAACCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC 
AACCCCATCG ACCTGCTGGA GTCCACCCTG TACGAGTCCT CCGTGGTGCC CGGGCCCAAG 
AAGGCGCCCA TGGACTCGCT GTTTGACTAC GGCACCTATC GTCACCACCC CAGTGACAAC 
AAGCGGTGGA GGAGGAAGGT CATAGAGAAG CAGCCACAGA GCCCCAAAGC TCCCGCACCC 
CAGCCGCCTC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG 
GGCTCCACAG CTGACCTGGA TGGGCTGCTC CCCTTCTTGC TGACCCACAA GAAGCGCCTC 
ACGGACGAGG AGTTCCGGGA ACCGTCCACC GGGAAGACCT GCCTGCCCAA GGCCCTGCTG 
AATCTGAGCA ACGGCCGCAA TGACACCATC CCTGTGCTTC TGGACATCGC CGAGCGCACG 
GGCAACATGC GGGAATTCAT CAACTCGCCC TTCCGAGACA TCTACTACCG AGGCCAGACC 
GCCCTGCACA TCGCCATCGA ACGCCGCTGC AAACACTATG TGGAGCTGCT GGTGGCCCAA 
GGAGCCGACG TCCATGCCCA GGCGCGGGGC CGCTTCTTCC AGCCCAAGGA CGAGGGCGGC 
TACTTCTACT TCGGTGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATC 
GTCAACTACC TGACGGAGAA CCCGCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGC 
GGCAACACGG TGCTGCACGC GCTGGTGGCC ATTGCCGACA ACACCCGCGA GAACACCAAG 
TTCGTCACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC 
AACCTGGAGG CCGTGCTCAA CAATGACGGC CTCTCGCCCC TCATGATGGC CGCCAAGACG 
GGCAAGATTG GGATCTTCCA ACACATCATC CGTCGGGAGG TGACAGACGA GGACACCAGG 
CACCTGTCGC GCAAGTTCAA GGACTGGGCC TATGGGCCGG TGTACTCCTC GCTCTACGAC 
CTCTCCTCCC TGGACACATG TGGGGAGGAG ACCTCTGTGC TGGAGATCCT GGTGTACAAC 
AGCAAGATCG AGAACCGCCA CGAGATGCTG GCCGTGGAAC CCATCAACGA ACTACTGCGG 
GACAAGTGGC GGAAGTTCGG GGCTGTCTCC TTCTACATCA ACGTGGTCTC CTATCTGTGT 
GCCATGGTCA TCTTTACCCT CACCGCCTAC TACCAGCCTC TCGAGGGCAC TCCGCCATAC 
CCTTACCGCA CCACAATGGA CTACCTGAGG CTGGCCGGCG AGATCATCAC ACTCTTCACT 
GGGATCCTGT TCTTTTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG 
AACTCTCTTT TCATCGACGG CTCCTTCCAG CTACTCTACT TCATCTACTC TGTGCTGGTG 
ATTGTCTCAG CGGCCCTCTA CCTGGCGGGG ATCGAGGCGT ACCTGGCCGT CATGGTCTTT 
GCCTTGGTCC TGGGCTGGAT GAACGCCCTT TACTTCACCC GCGGGCTGAA GCTGACGGGG 
ACCTATAGCA TCATGATCCA GAAGATCCTC TTCAAAGACC TTTTCCGCTT CCTGCTGGTC 
TACTTGCTCT TCATGATCGG CTACGCGTCA GCCCTGGTCT CGCTCCTGAA CCCGTGTGCC 
AACTTGAAGG TGTGCGATGA GGACCACGCC AACTGCACAG TGCCCACCTA CCCCTCATGC 
CGCGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTCT TCAAGCTCAC CATCGGCATG 
GGTGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTC 
ACGTACATCA TCCTCACATT CGTGCTGCTC CTTAACATGC TCATCGCCCT CATGGGGGAG 
ACAGTGGGCC AGGTGTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC 
ATCCTGGACA TCGAGCGCTC CTTCCCCGTG TTCCTGAGGA AGGCCTTCCG CTCCGGTGAA 
ATGGTGACCG TGGGCAAGAG CTCGGACGGC ACTCCAGACC GCAGGTGGTG CTTCAGGGTG 
GACGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATTAACGA GGACCCCGGC 
AAGAACGAGA ACTACCAGTA CTATGGCTTC TCGCACACCG TGGGCCGCCT CCGGAGGGAT 
CGCTGGTCCT CAGTGGTGCC CCGCGTGGTA GAGCTCAACA AGAACTCCAA CCCGGACGAG 
GTGGTGGTGC CTCTGGACAA CGTAGGGAAC CCCAGCTGCG ACGGCCACCA GCAGAGTTAC 
CCCCCTAAGT GGAGGACTGA CGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217959.1
CDS	114..2729
Translation	MADPGEGPRVGPGEMAESPGDESGPPGGEAFPLSSLANLFEGEDGSPSPSPADGGRSTGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHPSDNKRWRRKVIEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTIPVLLDIAERTGNMREFINSPFRDIYYRGQTALHIAIERRCKHYVELLVAQGADVHAQARGRFFQPKDEGGYFYFGELPLSLAACTNQPHIVNYLTENPHKKADMRRQDSRGNTVLHALVAIADNTRENTKFVTKMYDLLLLKCARLFPDSNLEAVLNNDGLSPLMMAAKTGKIGIFQHIIRREVTDEDTRHLSRKFKDWAYGPVYSSLYDLSSLDTCGEETSVLEILVYNSKIENRHEMLAVEPINELLRDKWRKFGAVSFYINVVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTMDYLRLAGEIITLFTGILFFFTNIKDLFMKKCPGVNSLFIDGSFQLLYFIYSVLVIVSAALYLAGIEAYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVSLLNPCANLKVCDEDHANCTVPTYPSCRDSETFSTFLLDLFKLTIGMGDLEMLSSTKYPVVFIILLVTYIILTFVLLLNMLIALMGETVGQVSKESKHIWKLQWATTILDIERSFPVFLRKAFRSGEMVTVGKSSDGTPDRRWCFRVDEVNWSHWNQNLGIINEDPGKNENYQYYGFSHTVGRLRRDRWSSVVPRVVELNKNSNPDEVVVPLDNVGNPSCDGHQQSYPPKWRTDDAPL

Target ORF information:

RefSeq Version	XM_005217902.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217902.2

ORF Insert Sequence:

ATGGCGGATC CTGGCGAAGG CCCTCGTGTG GGGCCAGGGG AGATGGCCGA GTCGCCCGGG 
GATGAGAGTG GGCCCCCCGG CGGGGAGGCC TTCCCCCTGT CATCACTGGC CAACCTGTTC 
GAGGGGGAAG ACGGCTCCCC GTCGCCCTCG CCAGCTGATG GCGGTCGCTC CACCGGCCCA 
GGCGACGGGC GACCCAACCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC 
AACCCCATCG ACCTGCTGGA GTCCACCCTG TACGAGTCCT CCGTGGTGCC CGGGCCCAAG 
AAGGCGCCCA TGGACTCGCT GTTTGACTAC GGCACCTATC GTCACCACCC CAGTGACAAC 
AAGCGGTGGA GGAGGAAGGT CATAGAGAAG CAGCCACAGA GCCCCAAAGC TCCCGCACCC 
CAGCCGCCTC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG 
GGCTCCACAG CTGACCTGGA TGGGCTGCTC CCCTTCTTGC TGACCCACAA GAAGCGCCTC 
ACGGACGAGG AGTTCCGGGA ACCGTCCACC GGGAAGACCT GCCTGCCCAA GGCCCTGCTG 
AATCTGAGCA ACGGCCGCAA TGACACCATC CCTGTGCTTC TGGACATCGC CGAGCGCACG 
GGCAACATGC GGGAATTCAT CAACTCGCCC TTCCGAGACA TCTACTACCG AGGCCAGACC 
GCCCTGCACA TCGCCATCGA ACGCCGCTGC AAACACTATG TGGAGCTGCT GGTGGCCCAA 
GGAGCCGACG TCCATGCCCA GGCGCGGGGC CGCTTCTTCC AGCCCAAGGA CGAGGGCGGC 
TACTTCTACT TCGGTGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATC 
GTCAACTACC TGACGGAGAA CCCGCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGC 
GGCAACACGG TGCTGCACGC GCTGGTGGCC ATTGCCGACA ACACCCGCGA GAACACCAAG 
TTCGTCACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC 
AACCTGGAGG CCGTGCTCAA CAATGACGGC CTCTCGCCCC TCATGATGGC CGCCAAGACG 
GGCAAGATTG GGATCTTCCA ACACATCATC CGTCGGGAGG TGACAGACGA GGACACCAGG 
CACCTGTCGC GCAAGTTCAA GGACTGGGCC TATGGGCCGG TGTACTCCTC GCTCTACGAC 
CTCTCCTCCC TGGACACATG TGGGGAGGAG ACCTCTGTGC TGGAGATCCT GGTGTACAAC 
AGCAAGATCG AGAACCGCCA CGAGATGCTG GCCGTGGAAC CCATCAACGA ACTACTGCGG 
GACAAGTGGC GGAAGTTCGG GGCTGTCTCC TTCTACATCA ACGTGGTCTC CTATCTGTGT 
GCCATGGTCA TCTTTACCCT CACCGCCTAC TACCAGCCTC TCGAGGGCAC TCCGCCATAC 
CCTTACCGCA CCACAATGGA CTACCTGAGG CTGGCCGGCG AGATCATCAC ACTCTTCACT 
GGGATCCTGT TCTTTTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG 
AACTCTCTTT TCATCGACGG CTCCTTCCAG CTACTCTACT TCATCTACTC TGTGCTGGTG 
ATTGTCTCAG CGGCCCTCTA CCTGGCGGGG ATCGAGGCGT ACCTGGCCGT CATGGTCTTT 
GCCTTGGTCC TGGGCTGGAT GAACGCCCTT TACTTCACCC GCGGGCTGAA GCTGACGGGG 
ACCTATAGCA TCATGATCCA GAAGATCCTC TTCAAAGACC TTTTCCGCTT CCTGCTGGTC 
TACTTGCTCT TCATGATCGG CTACGCGTCA GCCCTGGTCT CGCTCCTGAA CCCGTGTGCC 
AACTTGAAGG TGTGCGATGA GGACCACGCC AACTGCACAG TGCCCACCTA CCCCTCATGC 
CGCGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTCT TCAAGCTCAC CATCGGCATG 
GGTGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTC 
ACGTACATCA TCCTCACATT CGTGCTGCTC CTTAACATGC TCATCGCCCT CATGGGGGAG 
ACAGTGGGCC AGGTGTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC 
ATCCTGGACA TCGAGCGCTC CTTCCCCGTG TTCCTGAGGA AGGCCTTCCG CTCCGGTGAA 
ATGGTGACCG TGGGCAAGAG CTCGGACGGC ACTCCAGACC GCAGGTGGTG CTTCAGGGTG 
GACGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATTAACGA GGACCCCGGC 
AAGAACGAGA ACTACCAGTA CTATGGCTTC TCGCACACCG TGGGCCGCCT CCGGAGGGAT 
CGCTGGTCCT CAGTGGTGCC CCGCGTGGTA GAGCTCAACA AGAACTCCAA CCCGGACGAG 
GTGGTGGTGC CTCTGGACAA CGTAGGGAAC CCCAGCTGCG ACGGCCACCA GCAGAGTTAC 
CCCCCTAAGT GGAGGACTGA CGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPV4 ( XM_005217903.3 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_005217960.1 Bos taurus transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X2, mRNA.

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CloneID	OBa27927
Clone ID Related Accession (Same CDS sequence)	NM_001192385.2 , XM_005217902.2 , XM_005217903.3
Accession Version	XM_005217903.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transient receptor potential cation channel subfamily V member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217903.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CTGGCGAAGG CCCTCGTGTG GGGCCAGGGG AGATGGCCGA GTCGCCCGGG 
GATGAGAGTG GGCCCCCCGG CGGGGAGGCC TTCCCCCTGT CATCACTGGC CAACCTGTTC 
GAGGGGGAAG ACGGCTCCCC GTCGCCCTCG CCAGCTGATG GCGGTCGCTC CACCGGCCCA 
GGCGACGGGC GACCCAACCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC 
AACCCCATCG ACCTGCTGGA GTCCACCCTG TACGAGTCCT CCGTGGTGCC CGGGCCCAAG 
AAGGCGCCCA TGGACTCGCT GTTTGACTAC GGCACCTATC GTCACCACCC CAGTGACAAC 
AAGCGGTGGA GGAGGAAGGT CATAGAGAAG CAGCCACAGA GCCCCAAAGC TCCCGCACCC 
CAGCCGCCTC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG 
GGCTCCACAG CTGACCTGGA TGGGCTGCTC CCCTTCTTGC TGACCCACAA GAAGCGCCTC 
ACGGACGAGG AGTTCCGGGA ACCGTCCACC GGGAAGACCT GCCTGCCCAA GGCCCTGCTG 
AATCTGAGCA ACGGCCGCAA TGACACCATC CCTGTGCTTC TGGACATCGC CGAGCGCACG 
GGCAACATGC GGGAATTCAT CAACTCGCCC TTCCGAGACA TCTACTACCG AGGCCAGACC 
GCCCTGCACA TCGCCATCGA ACGCCGCTGC AAACACTATG TGGAGCTGCT GGTGGCCCAA 
GGAGCCGACG TCCATGCCCA GGCGCGGGGC CGCTTCTTCC AGCCCAAGGA CGAGGGCGGC 
TACTTCTACT TCGGTGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATC 
GTCAACTACC TGACGGAGAA CCCGCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGC 
GGCAACACGG TGCTGCACGC GCTGGTGGCC ATTGCCGACA ACACCCGCGA GAACACCAAG 
TTCGTCACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC 
AACCTGGAGG CCGTGCTCAA CAATGACGGC CTCTCGCCCC TCATGATGGC CGCCAAGACG 
GGCAAGATTG GGATCTTCCA ACACATCATC CGTCGGGAGG TGACAGACGA GGACACCAGG 
CACCTGTCGC GCAAGTTCAA GGACTGGGCC TATGGGCCGG TGTACTCCTC GCTCTACGAC 
CTCTCCTCCC TGGACACATG TGGGGAGGAG ACCTCTGTGC TGGAGATCCT GGTGTACAAC 
AGCAAGATCG AGAACCGCCA CGAGATGCTG GCCGTGGAAC CCATCAACGA ACTACTGCGG 
GACAAGTGGC GGAAGTTCGG GGCTGTCTCC TTCTACATCA ACGTGGTCTC CTATCTGTGT 
GCCATGGTCA TCTTTACCCT CACCGCCTAC TACCAGCCTC TCGAGGGCAC TCCGCCATAC 
CCTTACCGCA CCACAATGGA CTACCTGAGG CTGGCCGGCG AGATCATCAC ACTCTTCACT 
GGGATCCTGT TCTTTTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG 
AACTCTCTTT TCATCGACGG CTCCTTCCAG CTACTCTACT TCATCTACTC TGTGCTGGTG 
ATTGTCTCAG CGGCCCTCTA CCTGGCGGGG ATCGAGGCGT ACCTGGCCGT CATGGTCTTT 
GCCTTGGTCC TGGGCTGGAT GAACGCCCTT TACTTCACCC GCGGGCTGAA GCTGACGGGG 
ACCTATAGCA TCATGATCCA GAAGATCCTC TTCAAAGACC TTTTCCGCTT CCTGCTGGTC 
TACTTGCTCT TCATGATCGG CTACGCGTCA GCCCTGGTCT CGCTCCTGAA CCCGTGTGCC 
AACTTGAAGG TGTGCGATGA GGACCACGCC AACTGCACAG TGCCCACCTA CCCCTCATGC 
CGCGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTCT TCAAGCTCAC CATCGGCATG 
GGTGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTC 
ACGTACATCA TCCTCACATT CGTGCTGCTC CTTAACATGC TCATCGCCCT CATGGGGGAG 
ACAGTGGGCC AGGTGTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC 
ATCCTGGACA TCGAGCGCTC CTTCCCCGTG TTCCTGAGGA AGGCCTTCCG CTCCGGTGAA 
ATGGTGACCG TGGGCAAGAG CTCGGACGGC ACTCCAGACC GCAGGTGGTG CTTCAGGGTG 
GACGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATTAACGA GGACCCCGGC 
AAGAACGAGA ACTACCAGTA CTATGGCTTC TCGCACACCG TGGGCCGCCT CCGGAGGGAT 
CGCTGGTCCT CAGTGGTGCC CCGCGTGGTA GAGCTCAACA AGAACTCCAA CCCGGACGAG 
GTGGTGGTGC CTCTGGACAA CGTAGGGAAC CCCAGCTGCG ACGGCCACCA GCAGAGTTAC 
CCCCCTAAGT GGAGGACTGA CGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217960.1
CDS	298..2913
Translation	MADPGEGPRVGPGEMAESPGDESGPPGGEAFPLSSLANLFEGEDGSPSPSPADGGRSTGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHPSDNKRWRRKVIEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTIPVLLDIAERTGNMREFINSPFRDIYYRGQTALHIAIERRCKHYVELLVAQGADVHAQARGRFFQPKDEGGYFYFGELPLSLAACTNQPHIVNYLTENPHKKADMRRQDSRGNTVLHALVAIADNTRENTKFVTKMYDLLLLKCARLFPDSNLEAVLNNDGLSPLMMAAKTGKIGIFQHIIRREVTDEDTRHLSRKFKDWAYGPVYSSLYDLSSLDTCGEETSVLEILVYNSKIENRHEMLAVEPINELLRDKWRKFGAVSFYINVVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTMDYLRLAGEIITLFTGILFFFTNIKDLFMKKCPGVNSLFIDGSFQLLYFIYSVLVIVSAALYLAGIEAYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVSLLNPCANLKVCDEDHANCTVPTYPSCRDSETFSTFLLDLFKLTIGMGDLEMLSSTKYPVVFIILLVTYIILTFVLLLNMLIALMGETVGQVSKESKHIWKLQWATTILDIERSFPVFLRKAFRSGEMVTVGKSSDGTPDRRWCFRVDEVNWSHWNQNLGIINEDPGKNENYQYYGFSHTVGRLRRDRWSSVVPRVVELNKNSNPDEVVVPLDNVGNPSCDGHQQSYPPKWRTDDAPL

Target ORF information:

RefSeq Version	XM_005217903.3
Organism	Bos taurus(cattle)
Definition	Bos taurus transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217903.3

ORF Insert Sequence:

ATGGCGGATC CTGGCGAAGG CCCTCGTGTG GGGCCAGGGG AGATGGCCGA GTCGCCCGGG 
GATGAGAGTG GGCCCCCCGG CGGGGAGGCC TTCCCCCTGT CATCACTGGC CAACCTGTTC 
GAGGGGGAAG ACGGCTCCCC GTCGCCCTCG CCAGCTGATG GCGGTCGCTC CACCGGCCCA 
GGCGACGGGC GACCCAACCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC 
AACCCCATCG ACCTGCTGGA GTCCACCCTG TACGAGTCCT CCGTGGTGCC CGGGCCCAAG 
AAGGCGCCCA TGGACTCGCT GTTTGACTAC GGCACCTATC GTCACCACCC CAGTGACAAC 
AAGCGGTGGA GGAGGAAGGT CATAGAGAAG CAGCCACAGA GCCCCAAAGC TCCCGCACCC 
CAGCCGCCTC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG 
GGCTCCACAG CTGACCTGGA TGGGCTGCTC CCCTTCTTGC TGACCCACAA GAAGCGCCTC 
ACGGACGAGG AGTTCCGGGA ACCGTCCACC GGGAAGACCT GCCTGCCCAA GGCCCTGCTG 
AATCTGAGCA ACGGCCGCAA TGACACCATC CCTGTGCTTC TGGACATCGC CGAGCGCACG 
GGCAACATGC GGGAATTCAT CAACTCGCCC TTCCGAGACA TCTACTACCG AGGCCAGACC 
GCCCTGCACA TCGCCATCGA ACGCCGCTGC AAACACTATG TGGAGCTGCT GGTGGCCCAA 
GGAGCCGACG TCCATGCCCA GGCGCGGGGC CGCTTCTTCC AGCCCAAGGA CGAGGGCGGC 
TACTTCTACT TCGGTGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATC 
GTCAACTACC TGACGGAGAA CCCGCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGC 
GGCAACACGG TGCTGCACGC GCTGGTGGCC ATTGCCGACA ACACCCGCGA GAACACCAAG 
TTCGTCACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC 
AACCTGGAGG CCGTGCTCAA CAATGACGGC CTCTCGCCCC TCATGATGGC CGCCAAGACG 
GGCAAGATTG GGATCTTCCA ACACATCATC CGTCGGGAGG TGACAGACGA GGACACCAGG 
CACCTGTCGC GCAAGTTCAA GGACTGGGCC TATGGGCCGG TGTACTCCTC GCTCTACGAC 
CTCTCCTCCC TGGACACATG TGGGGAGGAG ACCTCTGTGC TGGAGATCCT GGTGTACAAC 
AGCAAGATCG AGAACCGCCA CGAGATGCTG GCCGTGGAAC CCATCAACGA ACTACTGCGG 
GACAAGTGGC GGAAGTTCGG GGCTGTCTCC TTCTACATCA ACGTGGTCTC CTATCTGTGT 
GCCATGGTCA TCTTTACCCT CACCGCCTAC TACCAGCCTC TCGAGGGCAC TCCGCCATAC 
CCTTACCGCA CCACAATGGA CTACCTGAGG CTGGCCGGCG AGATCATCAC ACTCTTCACT 
GGGATCCTGT TCTTTTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG 
AACTCTCTTT TCATCGACGG CTCCTTCCAG CTACTCTACT TCATCTACTC TGTGCTGGTG 
ATTGTCTCAG CGGCCCTCTA CCTGGCGGGG ATCGAGGCGT ACCTGGCCGT CATGGTCTTT 
GCCTTGGTCC TGGGCTGGAT GAACGCCCTT TACTTCACCC GCGGGCTGAA GCTGACGGGG 
ACCTATAGCA TCATGATCCA GAAGATCCTC TTCAAAGACC TTTTCCGCTT CCTGCTGGTC 
TACTTGCTCT TCATGATCGG CTACGCGTCA GCCCTGGTCT CGCTCCTGAA CCCGTGTGCC 
AACTTGAAGG TGTGCGATGA GGACCACGCC AACTGCACAG TGCCCACCTA CCCCTCATGC 
CGCGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTCT TCAAGCTCAC CATCGGCATG 
GGTGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTC 
ACGTACATCA TCCTCACATT CGTGCTGCTC CTTAACATGC TCATCGCCCT CATGGGGGAG 
ACAGTGGGCC AGGTGTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC 
ATCCTGGACA TCGAGCGCTC CTTCCCCGTG TTCCTGAGGA AGGCCTTCCG CTCCGGTGAA 
ATGGTGACCG TGGGCAAGAG CTCGGACGGC ACTCCAGACC GCAGGTGGTG CTTCAGGGTG 
GACGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATTAACGA GGACCCCGGC 
AAGAACGAGA ACTACCAGTA CTATGGCTTC TCGCACACCG TGGGCCGCCT CCGGAGGGAT 
CGCTGGTCCT CAGTGGTGCC CCGCGTGGTA GAGCTCAACA AGAACTCCAA CCCGGACGAG 
GTGGTGGTGC CTCTGGACAA CGTAGGGAAC CCCAGCTGCG ACGGCCACCA GCAGAGTTAC 
CCCCCTAAGT GGAGGACTGA CGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPV4 ( XM_005217902.3 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_005217959.1 Bos taurus transient receptor potential cation channel subfamily V member 4 (TRPV4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa38167
Clone ID Related Accession (Same CDS sequence)	XM_005217902.3 , XM_005217903.4 , NM_001192385.3 , NM_001192385.1
Accession Version	XM_005217902.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transient receptor potential cation channel subfamily V member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217902.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CTGGCGAAGG CCCTCGTGTG GGGCCAGGGG AGATGGCCGA GTCGCCCGGG 
GATGAGAGTG GGCCCCCCGG CGGGGAGGCC TTCCCCCTGT CATCACTGGC CAACCTGTTC 
GAGGGGGAAG ACGGCTCCCC GTCGCCCTCG CCAGCTGATG GCGGTCGCTC CACCGGCCCA 
GGCGACGGGC GACCCAACCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC 
AACCCCATCG ACCTGCTGGA GTCCACCCTG TACGAGTCCT CCGTGGTGCC CGGGCCCAAG 
AAGGCGCCCA TGGACTCGCT GTTTGACTAC GGCACCTATC GTCACCACCC CAGTGACAAC 
AAGCGGTGGA GGAGGAAGGT CATAGAGAAG CAGCCACAGA GCCCCAAAGC TCCCGCACCC 
CAGCCGCCTC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG 
GGCTCCACAG CTGACCTGGA TGGGCTGCTC CCCTTCTTGC TGACCCACAA GAAGCGCCTC 
ACGGACGAGG AGTTCCGGGA ACCGTCCACC GGGAAGACCT GCCTGCCCAA GGCCCTGCTG 
AATCTGAGCA ACGGCCGCAA TGACACCATC CCTGTGCTTC TGGACATCGC CGAGCGCACG 
GGCAACATGC GGGAATTCAT CAACTCGCCC TTCCGAGACA TCTACTACCG AGGCCAGACC 
GCCCTGCACA TCGCCATCGA ACGCCGCTGC AAACACTATG TGGAGCTGCT GGTGGCCCAA 
GGAGCCGACG TCCATGCCCA GGCGCGGGGC CGCTTCTTCC AGCCCAAGGA CGAGGGCGGC 
TACTTCTACT TCGGTGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATC 
GTCAACTACC TGACGGAGAA CCCGCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGC 
GGCAACACGG TGCTGCACGC ACTGGTGGCC ATTGCCGACA ACACCCGCGA GAACACCAAG 
TTCGTCACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC 
AACCTGGAGG CCGTGCTCAA CAATGACGGC CTCTCGCCCC TCATGATGGC CGCCAAGACG 
GGCAAGATTG GGATCTTCCA ACACATCATC CGTCGGGAGG TGACAGACGA GGACACCAGG 
CACCTGTCGC GCAAGTTCAA GGACTGGGCC TATGGGCCGG TGTACTCCTC GCTCTACGAC 
CTCTCCTCCC TGGACACATG TGGGGAGGAG ACCTCTGTGC TGGAGATCCT GGTGTACAAC 
AGCAAGATCG AGAACCGCCA CGAGATGCTG GCCGTGGAAC CCATCAACGA ACTACTGCGG 
GACAAGTGGC GGAAGTTCGG GGCTGTCTCC TTCTACATCA ACGTGGTCTC CTATCTGTGT 
GCCATGGTCA TCTTTACCCT CACCGCCTAC TACCAGCCTC TCGAGGGCAC TCCGCCATAC 
CCTTACCGCA CCACAATGGA CTACCTGAGG CTGGCCGGCG AGATCATCAC ACTCTTCACT 
GGGATCCTGT TCTTTTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG 
AACTCTCTTT TCATCGACGG CTCCTTCCAG CTACTCTACT TCATCTACTC TGTGCTGGTG 
ATTGTCTCAG CGGCCCTCTA CCTGGCGGGG ATCGAGGCGT ACCTGGCCGT CATGGTCTTT 
GCCTTGGTCC TGGGCTGGAT GAACGCCCTT TACTTCACCC GCGGGCTGAA GCTGACGGGG 
ACCTATAGCA TCATGATCCA GAAGATCCTC TTCAAAGACC TTTTCCGCTT CCTGCTGGTC 
TACTTGCTCT TCATGATCGG CTACGCGTCA GCCCTGGTCT CGCTCCTGAA CCCGTGTGCC 
AACTTGAAGG TGTGCGATGA GGACCACGCC AACTGCACAG TGCCCACCTA CCCCTCATGC 
CGCGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTCT TCAAGCTCAC CATCGGCATG 
GGTGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTC 
ACGTACATCA TCCTCACATT CGTGCTGCTC CTTAACATGC TCATCGCCCT CATGGGGGAG 
ACAGTGGGCC AGGTGTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC 
ATCCTGGACA TCGAGCGCTC CTTCCCCGTG TTCCTGAGGA AGGCCTTCCG CTCCGGTGAA 
ATGGTGACCG TGGGCAAGAG CTCGGACGGC ACTCCAGACC GCAGGTGGTG CTTCAGGGTG 
GACGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATTAACGA GGACCCCGGC 
AAGAACGAGA ACTACCAGTA CTATGGCTTC TCGCACACCG TGGGCCGCCT CCGGAGGGAT 
CGCTGGTCCT CAGTGGTGCC CCGCGTGGTA GAGCTCAACA AGAACTCCAA CCCGGACGAG 
GTGGTGGTGC CTCTGGACAA CGTAGGGAAC CCCAGCTGCG ACGGCCACCA GCAGAGTTAC 
CCCCCTAAGT GGAGGACTGA CGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217959.1
CDS	114..2729
Translation	MADPGEGPRVGPGEMAESPGDESGPPGGEAFPLSSLANLFEGEDGSPSPSPADGGRSTGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHPSDNKRWRRKVIEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTIPVLLDIAERTGNMREFINSPFRDIYYRGQTALHIAIERRCKHYVELLVAQGADVHAQARGRFFQPKDEGGYFYFGELPLSLAACTNQPHIVNYLTENPHKKADMRRQDSRGNTVLHALVAIADNTRENTKFVTKMYDLLLLKCARLFPDSNLEAVLNNDGLSPLMMAAKTGKIGIFQHIIRREVTDEDTRHLSRKFKDWAYGPVYSSLYDLSSLDTCGEETSVLEILVYNSKIENRHEMLAVEPINELLRDKWRKFGAVSFYINVVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTMDYLRLAGEIITLFTGILFFFTNIKDLFMKKCPGVNSLFIDGSFQLLYFIYSVLVIVSAALYLAGIEAYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVSLLNPCANLKVCDEDHANCTVPTYPSCRDSETFSTFLLDLFKLTIGMGDLEMLSSTKYPVVFIILLVTYIILTFVLLLNMLIALMGETVGQVSKESKHIWKLQWATTILDIERSFPVFLRKAFRSGEMVTVGKSSDGTPDRRWCFRVDEVNWSHWNQNLGIINEDPGKNENYQYYGFSHTVGRLRRDRWSSVVPRVVELNKNSNPDEVVVPLDNVGNPSCDGHQQSYPPKWRTDDAPL

Target ORF information:

RefSeq Version	XM_005217902.3
Organism	Bos taurus(cattle)
Definition	Bos taurus transient receptor potential cation channel subfamily V member 4 (TRPV4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217902.3

ORF Insert Sequence:

ATGGCGGATC CTGGCGAAGG CCCTCGTGTG GGGCCAGGGG AGATGGCCGA GTCGCCCGGG 
GATGAGAGTG GGCCCCCCGG CGGGGAGGCC TTCCCCCTGT CATCACTGGC CAACCTGTTC 
GAGGGGGAAG ACGGCTCCCC GTCGCCCTCG CCAGCTGATG GCGGTCGCTC CACCGGCCCA 
GGCGACGGGC GACCCAACCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC 
AACCCCATCG ACCTGCTGGA GTCCACCCTG TACGAGTCCT CCGTGGTGCC CGGGCCCAAG 
AAGGCGCCCA TGGACTCGCT GTTTGACTAC GGCACCTATC GTCACCACCC CAGTGACAAC 
AAGCGGTGGA GGAGGAAGGT CATAGAGAAG CAGCCACAGA GCCCCAAAGC TCCCGCACCC 
CAGCCGCCTC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG 
GGCTCCACAG CTGACCTGGA TGGGCTGCTC CCCTTCTTGC TGACCCACAA GAAGCGCCTC 
ACGGACGAGG AGTTCCGGGA ACCGTCCACC GGGAAGACCT GCCTGCCCAA GGCCCTGCTG 
AATCTGAGCA ACGGCCGCAA TGACACCATC CCTGTGCTTC TGGACATCGC CGAGCGCACG 
GGCAACATGC GGGAATTCAT CAACTCGCCC TTCCGAGACA TCTACTACCG AGGCCAGACC 
GCCCTGCACA TCGCCATCGA ACGCCGCTGC AAACACTATG TGGAGCTGCT GGTGGCCCAA 
GGAGCCGACG TCCATGCCCA GGCGCGGGGC CGCTTCTTCC AGCCCAAGGA CGAGGGCGGC 
TACTTCTACT TCGGTGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATC 
GTCAACTACC TGACGGAGAA CCCGCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGC 
GGCAACACGG TGCTGCACGC ACTGGTGGCC ATTGCCGACA ACACCCGCGA GAACACCAAG 
TTCGTCACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC 
AACCTGGAGG CCGTGCTCAA CAATGACGGC CTCTCGCCCC TCATGATGGC CGCCAAGACG 
GGCAAGATTG GGATCTTCCA ACACATCATC CGTCGGGAGG TGACAGACGA GGACACCAGG 
CACCTGTCGC GCAAGTTCAA GGACTGGGCC TATGGGCCGG TGTACTCCTC GCTCTACGAC 
CTCTCCTCCC TGGACACATG TGGGGAGGAG ACCTCTGTGC TGGAGATCCT GGTGTACAAC 
AGCAAGATCG AGAACCGCCA CGAGATGCTG GCCGTGGAAC CCATCAACGA ACTACTGCGG 
GACAAGTGGC GGAAGTTCGG GGCTGTCTCC TTCTACATCA ACGTGGTCTC CTATCTGTGT 
GCCATGGTCA TCTTTACCCT CACCGCCTAC TACCAGCCTC TCGAGGGCAC TCCGCCATAC 
CCTTACCGCA CCACAATGGA CTACCTGAGG CTGGCCGGCG AGATCATCAC ACTCTTCACT 
GGGATCCTGT TCTTTTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG 
AACTCTCTTT TCATCGACGG CTCCTTCCAG CTACTCTACT TCATCTACTC TGTGCTGGTG 
ATTGTCTCAG CGGCCCTCTA CCTGGCGGGG ATCGAGGCGT ACCTGGCCGT CATGGTCTTT 
GCCTTGGTCC TGGGCTGGAT GAACGCCCTT TACTTCACCC GCGGGCTGAA GCTGACGGGG 
ACCTATAGCA TCATGATCCA GAAGATCCTC TTCAAAGACC TTTTCCGCTT CCTGCTGGTC 
TACTTGCTCT TCATGATCGG CTACGCGTCA GCCCTGGTCT CGCTCCTGAA CCCGTGTGCC 
AACTTGAAGG TGTGCGATGA GGACCACGCC AACTGCACAG TGCCCACCTA CCCCTCATGC 
CGCGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTCT TCAAGCTCAC CATCGGCATG 
GGTGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTC 
ACGTACATCA TCCTCACATT CGTGCTGCTC CTTAACATGC TCATCGCCCT CATGGGGGAG 
ACAGTGGGCC AGGTGTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC 
ATCCTGGACA TCGAGCGCTC CTTCCCCGTG TTCCTGAGGA AGGCCTTCCG CTCCGGTGAA 
ATGGTGACCG TGGGCAAGAG CTCGGACGGC ACTCCAGACC GCAGGTGGTG CTTCAGGGTG 
GACGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATTAACGA GGACCCCGGC 
AAGAACGAGA ACTACCAGTA CTATGGCTTC TCGCACACCG TGGGCCGCCT CCGGAGGGAT 
CGCTGGTCCT CAGTGGTGCC CCGCGTGGTA GAGCTCAACA AGAACTCCAA CCCGGACGAG 
GTGGTGGTGC CTCTGGACAA CGTAGGGAAC CCCAGCTGCG ACGGCCACCA GCAGAGTTAC 
CCCCCTAAGT GGAGGACTGA CGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPV4 ( XM_005217903.4 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_005217960.1 Bos taurus transient receptor potential cation channel subfamily V member 4 (TRPV4), transcript variant X2, mRNA.

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CloneID	OBa38167
Clone ID Related Accession (Same CDS sequence)	XM_005217902.3 , XM_005217903.4 , NM_001192385.3 , NM_001192385.1
Accession Version	XM_005217903.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transient receptor potential cation channel subfamily V member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217903.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CTGGCGAAGG CCCTCGTGTG GGGCCAGGGG AGATGGCCGA GTCGCCCGGG 
GATGAGAGTG GGCCCCCCGG CGGGGAGGCC TTCCCCCTGT CATCACTGGC CAACCTGTTC 
GAGGGGGAAG ACGGCTCCCC GTCGCCCTCG CCAGCTGATG GCGGTCGCTC CACCGGCCCA 
GGCGACGGGC GACCCAACCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC 
AACCCCATCG ACCTGCTGGA GTCCACCCTG TACGAGTCCT CCGTGGTGCC CGGGCCCAAG 
AAGGCGCCCA TGGACTCGCT GTTTGACTAC GGCACCTATC GTCACCACCC CAGTGACAAC 
AAGCGGTGGA GGAGGAAGGT CATAGAGAAG CAGCCACAGA GCCCCAAAGC TCCCGCACCC 
CAGCCGCCTC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG 
GGCTCCACAG CTGACCTGGA TGGGCTGCTC CCCTTCTTGC TGACCCACAA GAAGCGCCTC 
ACGGACGAGG AGTTCCGGGA ACCGTCCACC GGGAAGACCT GCCTGCCCAA GGCCCTGCTG 
AATCTGAGCA ACGGCCGCAA TGACACCATC CCTGTGCTTC TGGACATCGC CGAGCGCACG 
GGCAACATGC GGGAATTCAT CAACTCGCCC TTCCGAGACA TCTACTACCG AGGCCAGACC 
GCCCTGCACA TCGCCATCGA ACGCCGCTGC AAACACTATG TGGAGCTGCT GGTGGCCCAA 
GGAGCCGACG TCCATGCCCA GGCGCGGGGC CGCTTCTTCC AGCCCAAGGA CGAGGGCGGC 
TACTTCTACT TCGGTGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATC 
GTCAACTACC TGACGGAGAA CCCGCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGC 
GGCAACACGG TGCTGCACGC ACTGGTGGCC ATTGCCGACA ACACCCGCGA GAACACCAAG 
TTCGTCACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC 
AACCTGGAGG CCGTGCTCAA CAATGACGGC CTCTCGCCCC TCATGATGGC CGCCAAGACG 
GGCAAGATTG GGATCTTCCA ACACATCATC CGTCGGGAGG TGACAGACGA GGACACCAGG 
CACCTGTCGC GCAAGTTCAA GGACTGGGCC TATGGGCCGG TGTACTCCTC GCTCTACGAC 
CTCTCCTCCC TGGACACATG TGGGGAGGAG ACCTCTGTGC TGGAGATCCT GGTGTACAAC 
AGCAAGATCG AGAACCGCCA CGAGATGCTG GCCGTGGAAC CCATCAACGA ACTACTGCGG 
GACAAGTGGC GGAAGTTCGG GGCTGTCTCC TTCTACATCA ACGTGGTCTC CTATCTGTGT 
GCCATGGTCA TCTTTACCCT CACCGCCTAC TACCAGCCTC TCGAGGGCAC TCCGCCATAC 
CCTTACCGCA CCACAATGGA CTACCTGAGG CTGGCCGGCG AGATCATCAC ACTCTTCACT 
GGGATCCTGT TCTTTTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG 
AACTCTCTTT TCATCGACGG CTCCTTCCAG CTACTCTACT TCATCTACTC TGTGCTGGTG 
ATTGTCTCAG CGGCCCTCTA CCTGGCGGGG ATCGAGGCGT ACCTGGCCGT CATGGTCTTT 
GCCTTGGTCC TGGGCTGGAT GAACGCCCTT TACTTCACCC GCGGGCTGAA GCTGACGGGG 
ACCTATAGCA TCATGATCCA GAAGATCCTC TTCAAAGACC TTTTCCGCTT CCTGCTGGTC 
TACTTGCTCT TCATGATCGG CTACGCGTCA GCCCTGGTCT CGCTCCTGAA CCCGTGTGCC 
AACTTGAAGG TGTGCGATGA GGACCACGCC AACTGCACAG TGCCCACCTA CCCCTCATGC 
CGCGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTCT TCAAGCTCAC CATCGGCATG 
GGTGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTC 
ACGTACATCA TCCTCACATT CGTGCTGCTC CTTAACATGC TCATCGCCCT CATGGGGGAG 
ACAGTGGGCC AGGTGTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC 
ATCCTGGACA TCGAGCGCTC CTTCCCCGTG TTCCTGAGGA AGGCCTTCCG CTCCGGTGAA 
ATGGTGACCG TGGGCAAGAG CTCGGACGGC ACTCCAGACC GCAGGTGGTG CTTCAGGGTG 
GACGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATTAACGA GGACCCCGGC 
AAGAACGAGA ACTACCAGTA CTATGGCTTC TCGCACACCG TGGGCCGCCT CCGGAGGGAT 
CGCTGGTCCT CAGTGGTGCC CCGCGTGGTA GAGCTCAACA AGAACTCCAA CCCGGACGAG 
GTGGTGGTGC CTCTGGACAA CGTAGGGAAC CCCAGCTGCG ACGGCCACCA GCAGAGTTAC 
CCCCCTAAGT GGAGGACTGA CGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217960.1
CDS	499..3114
Translation	MADPGEGPRVGPGEMAESPGDESGPPGGEAFPLSSLANLFEGEDGSPSPSPADGGRSTGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHPSDNKRWRRKVIEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTIPVLLDIAERTGNMREFINSPFRDIYYRGQTALHIAIERRCKHYVELLVAQGADVHAQARGRFFQPKDEGGYFYFGELPLSLAACTNQPHIVNYLTENPHKKADMRRQDSRGNTVLHALVAIADNTRENTKFVTKMYDLLLLKCARLFPDSNLEAVLNNDGLSPLMMAAKTGKIGIFQHIIRREVTDEDTRHLSRKFKDWAYGPVYSSLYDLSSLDTCGEETSVLEILVYNSKIENRHEMLAVEPINELLRDKWRKFGAVSFYINVVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTMDYLRLAGEIITLFTGILFFFTNIKDLFMKKCPGVNSLFIDGSFQLLYFIYSVLVIVSAALYLAGIEAYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVSLLNPCANLKVCDEDHANCTVPTYPSCRDSETFSTFLLDLFKLTIGMGDLEMLSSTKYPVVFIILLVTYIILTFVLLLNMLIALMGETVGQVSKESKHIWKLQWATTILDIERSFPVFLRKAFRSGEMVTVGKSSDGTPDRRWCFRVDEVNWSHWNQNLGIINEDPGKNENYQYYGFSHTVGRLRRDRWSSVVPRVVELNKNSNPDEVVVPLDNVGNPSCDGHQQSYPPKWRTDDAPL

Target ORF information:

RefSeq Version	XM_005217903.4
Organism	Bos taurus(cattle)
Definition	Bos taurus transient receptor potential cation channel subfamily V member 4 (TRPV4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217903.4

ORF Insert Sequence:

ATGGCGGATC CTGGCGAAGG CCCTCGTGTG GGGCCAGGGG AGATGGCCGA GTCGCCCGGG 
GATGAGAGTG GGCCCCCCGG CGGGGAGGCC TTCCCCCTGT CATCACTGGC CAACCTGTTC 
GAGGGGGAAG ACGGCTCCCC GTCGCCCTCG CCAGCTGATG GCGGTCGCTC CACCGGCCCA 
GGCGACGGGC GACCCAACCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC 
AACCCCATCG ACCTGCTGGA GTCCACCCTG TACGAGTCCT CCGTGGTGCC CGGGCCCAAG 
AAGGCGCCCA TGGACTCGCT GTTTGACTAC GGCACCTATC GTCACCACCC CAGTGACAAC 
AAGCGGTGGA GGAGGAAGGT CATAGAGAAG CAGCCACAGA GCCCCAAAGC TCCCGCACCC 
CAGCCGCCTC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG 
GGCTCCACAG CTGACCTGGA TGGGCTGCTC CCCTTCTTGC TGACCCACAA GAAGCGCCTC 
ACGGACGAGG AGTTCCGGGA ACCGTCCACC GGGAAGACCT GCCTGCCCAA GGCCCTGCTG 
AATCTGAGCA ACGGCCGCAA TGACACCATC CCTGTGCTTC TGGACATCGC CGAGCGCACG 
GGCAACATGC GGGAATTCAT CAACTCGCCC TTCCGAGACA TCTACTACCG AGGCCAGACC 
GCCCTGCACA TCGCCATCGA ACGCCGCTGC AAACACTATG TGGAGCTGCT GGTGGCCCAA 
GGAGCCGACG TCCATGCCCA GGCGCGGGGC CGCTTCTTCC AGCCCAAGGA CGAGGGCGGC 
TACTTCTACT TCGGTGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATC 
GTCAACTACC TGACGGAGAA CCCGCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGC 
GGCAACACGG TGCTGCACGC ACTGGTGGCC ATTGCCGACA ACACCCGCGA GAACACCAAG 
TTCGTCACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC 
AACCTGGAGG CCGTGCTCAA CAATGACGGC CTCTCGCCCC TCATGATGGC CGCCAAGACG 
GGCAAGATTG GGATCTTCCA ACACATCATC CGTCGGGAGG TGACAGACGA GGACACCAGG 
CACCTGTCGC GCAAGTTCAA GGACTGGGCC TATGGGCCGG TGTACTCCTC GCTCTACGAC 
CTCTCCTCCC TGGACACATG TGGGGAGGAG ACCTCTGTGC TGGAGATCCT GGTGTACAAC 
AGCAAGATCG AGAACCGCCA CGAGATGCTG GCCGTGGAAC CCATCAACGA ACTACTGCGG 
GACAAGTGGC GGAAGTTCGG GGCTGTCTCC TTCTACATCA ACGTGGTCTC CTATCTGTGT 
GCCATGGTCA TCTTTACCCT CACCGCCTAC TACCAGCCTC TCGAGGGCAC TCCGCCATAC 
CCTTACCGCA CCACAATGGA CTACCTGAGG CTGGCCGGCG AGATCATCAC ACTCTTCACT 
GGGATCCTGT TCTTTTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG 
AACTCTCTTT TCATCGACGG CTCCTTCCAG CTACTCTACT TCATCTACTC TGTGCTGGTG 
ATTGTCTCAG CGGCCCTCTA CCTGGCGGGG ATCGAGGCGT ACCTGGCCGT CATGGTCTTT 
GCCTTGGTCC TGGGCTGGAT GAACGCCCTT TACTTCACCC GCGGGCTGAA GCTGACGGGG 
ACCTATAGCA TCATGATCCA GAAGATCCTC TTCAAAGACC TTTTCCGCTT CCTGCTGGTC 
TACTTGCTCT TCATGATCGG CTACGCGTCA GCCCTGGTCT CGCTCCTGAA CCCGTGTGCC 
AACTTGAAGG TGTGCGATGA GGACCACGCC AACTGCACAG TGCCCACCTA CCCCTCATGC 
CGCGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTCT TCAAGCTCAC CATCGGCATG 
GGTGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTC 
ACGTACATCA TCCTCACATT CGTGCTGCTC CTTAACATGC TCATCGCCCT CATGGGGGAG 
ACAGTGGGCC AGGTGTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC 
ATCCTGGACA TCGAGCGCTC CTTCCCCGTG TTCCTGAGGA AGGCCTTCCG CTCCGGTGAA 
ATGGTGACCG TGGGCAAGAG CTCGGACGGC ACTCCAGACC GCAGGTGGTG CTTCAGGGTG 
GACGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATTAACGA GGACCCCGGC 
AAGAACGAGA ACTACCAGTA CTATGGCTTC TCGCACACCG TGGGCCGCCT CCGGAGGGAT 
CGCTGGTCCT CAGTGGTGCC CCGCGTGGTA GAGCTCAACA AGAACTCCAA CCCGGACGAG 
GTGGTGGTGC CTCTGGACAA CGTAGGGAAC CCCAGCTGCG ACGGCCACCA GCAGAGTTAC 
CCCCCTAAGT GGAGGACTGA CGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reduced tissue osmolarity increases TRPV4 expression and pro-inflammatory cytokines in intervertebral disc cells.
European cells & materials32123-36(2016 Jul)
Walter BA,Purmessur D,Moon A,Occhiogrosso J,Laudier DM,Hecht AC,Iatridis JC

TRPV4 channels activity in bovine articular chondrocytes: regulation by obesity-associated mediators.
Cell calcium56(6)493-503(2014 Dec)
S?nchez JC,L?pez-Zapata DF,Wilkins RJ

TRPV4 channels mediate cyclic strain-induced endothelial cell reorientation through integrin-to-integrin signaling.
Circulation research104(9)1123-30(2009 May)
Thodeti CK,Matthews B,Ravi A,Mammoto A,Ghosh K,Bracha AL,Ingber DE

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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TRPV4 cDNA ORF clone, Bos taurus(Cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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