trpv4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	trpv4
Entrez Gene ID	557850
Full Name	transient receptor potential cation channel, subfamily V, member 4
Synonyms	wu:fp52e02
General protein information	Preferred Names transient receptor potential cation channel, subfamily V, member 4 Names transient receptor potential cation channel subfamily V member 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005165151.4	XP_005165208.1	transient receptor potential cation channel subfamily V member 4 isoform X1
XM_005165151.3	XP_005165208.1	transient receptor potential cation channel subfamily V member 4 isoform X1
XM_005165152.3	XP_005165209.1	transient receptor potential cation channel subfamily V member 4 isoform X2
XM_009301307.2	XP_009299582.1	transient receptor potential cation channel subfamily V member 4 isoform X4
XM_009301306.2	XP_009299581.1	transient receptor potential cation channel subfamily V member 4 isoform X3
NM_001042730.1	NP_001036195.1	transient receptor potential cation channel subfamily V member 4
XM_005165152.4	XP_005165209.1	transient receptor potential cation channel subfamily V member 4 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRPV4	NP_067638.3
Canis lupus familiaris (dog)	TRPV4	NP_001120787.1
Bos taurus (cattle)	TRPV4	NP_001179314.1
Mus musculus (house mouse)	Trpv4	NP_071300.2
Xenopus tropicalis (tropical clawed frog)	trpv4	XP_002932129.1
Gallus gallus (chicken)	TRPV4	NP_990023.1
Pan troglodytes (chimpanzee)	TRPV4	XP_003314024.1
Rattus norvegicus (Norway rat)	Trpv4	NP_076460.1
Danio rerio (zebrafish)	trpv4	NP_001036195.1

Gene Ontology
Bibliography

Related articles in PubMed

Temperature-activated ion channels in neural crest cells confer maternal fever-associated birth defects.
Hutson MR, Keyte AL, Hern?ndez-Morales M, Gibbs E, Kupchinsky ZA, Argyridis I, Erwin KN, Pegram K, Kneifel M, Rosenberg PB, Matak P, Xie L, Grandl J, Davis EE, Katsanis N, Liu C, Benner EJ
Science signaling10(500)(2017 Oct)

Thermal stress in Danio rerio: a link between temperature, light, thermo-TRP channels, and clock genes.
Jer?nimo R, Moraes MN, de Assis LVM, Ramos BC, Rocha T, Castrucci AML
Journal of thermal biology68(Pt A)128-138(2017 Aug)

TRPV4-Mediated Detection of Hyposmotic Stress by Skin Keratinocytes Activates Developmental Immunity.
Galindo-Villegas J, Montalban-Arques A, Liarte S, de Oliveira S, Pardo-Pastor C, Rubio-Moscardo F, Meseguer J, Valverde MA, Mulero V
Journal of immunology (Baltimore, Md. : 1950)196(2)738-49(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TRPP2 and TRPV4 are mechanosensitive channels in the endocardium.Oscillatory flow modulates mechanosensitive klf2a expression through trpv4 and trpp2 during heart valve development.
Title: Oscillatory Flow Modulates Mechanosensitive klf2a Expression through trpv4 and trpp2 during Heart Valve Development.

Age-dependence of heat-activated seizure susceptibility mimicks the mRNA expression of TRPV4 and glutamate receptors.
Title: A novel zebrafish model of hyperthermia-induced seizures reveals a role for TRPV4 channels and NMDA-type glutamate receptors.

TRPV4 present in all sensory organs of adult zebrafish.
Title: TRPV4 in the sensory organs of adult zebrafish.

TRPP2 utilizes TRPV4 to form a mechano- and thermosensitive molecular sensor in the cilium.
Title: TRPP2 and TRPV4 form a polymodal sensory channel complex.

OS-9 regulates the secretory transport of TRPV4 and appears to protect TRPV4 subunits from the precocious ubiquitination and ER-associated degradation.
Title: OS-9 regulates the transit and polyubiquitination of TRPV4 in the endoplasmic reticulum.

The following trpv4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trpv4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa42687	XM_009301307.2 Latest version!	Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa04267	NM_001042730.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa10274	XM_005165151.4 Latest version!	Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa10275	XM_005165152.4 Latest version!	Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa42686	XM_009301306.2 Latest version!	Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa10274	XM_005165151.3	Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa10275	XM_005165152.3	Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trpv4 ( XM_009301307.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299582.1 Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa42687
Clone ID Related Accession (Same CDS sequence)	XM_009301307.2
Accession Version	XM_009301307.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily V member 4 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301307.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGACC AGGGTTTTTC TGCTTCCACT CTACTGAAGC GCTATCGTCT GGCCATGACA 
GAGTCCTTGT CTGTCTCCTC ACCACCAGAC AATTCAGCTC AGGACTCTTC AGAGGCAGCA 
GATGGAGATC CCAACTTCCC CATGTCTTCA ATGGCAGCGC TACTGGAAAA TGATGATGTA 
TCACAGCCAA CTCATGAATT GCCTCGACCA GGACAACAGA ACGACCAGAA GCAGAACATG 
CGGATTAGGT TCCCAGGACC CTTTAAAAAG GGGGTTCCTA ACCCTATGGA TCTTCTTGAG 
TCGGATTATA CAGAGTATCC TAAACAAGCA CCTATGGATT CCATGTTTGA CTACGGCACA 
TGCAGGCAGA TCAACAACAA TAAGAAAGGA AGACGGAAGA AGCTTCCACG AGGGAAGGCA 
GAGATCGGGA TGTCATGTGA TGAGGGATCC CCGGAGCCCC CTGTTCTCAA AGTCTTCAAC 
CGCTGGATGT TGTTCGAAGC TGTATCTCGA GCAGATCCAA GAGCTCTGGA CGGCCTTCTG 
CAGTATCTTC AGTCCCACGA AAAGAGGCTG ACCGATGAAG AGTTCAAAGA ACTTTCTACA 
GGGAAAACCT GCCTACCAAA AGCACTTCTC AACCTGCACA ATGGTCAAAA CGACACCATC 
CCAATATTGG TGGACATTGC TGAACAGACA GGAAACCTCA GAGAGTTCAT CAACACACCC 
TTTAGAGATG TCTATTATAG GGGTCAGATG GCTCTGCATA TTGCTATAGA GCGACGATGC 
AAACAGTATG TGGAGCTTCT GGTGGAGAAG GGGGCTGATG TCCATGCGCA GGCCAGGGGA 
CGCTTCTTCC AGCCCAGAGA TGAGGGTGGA TACTTCTACT TTGGTGAACT GCCCCTCTCA 
CTCGCAGCTT GTACCAACCA ACCAGACATG GTGCATTACC TGACAGAGAA TGGACACAAG 
AAGGCGGATC TGAGGAGGCA GGACTCAAGG GGAAACACAG TCCTGCATGC TTTGGTCCAC 
ATTGCTGACA ACACTCGGGA CAACACACGT TTTGTCACTA AGATGTTTGA CTTGCTGCTC 
ATTAAATGTG CCAAACTCTA CCCGGATTGC AACCTGGAGA ATATACTCAA CAACGACGGC 
ATGTCCCCTC TCATGATGGC CGCCAAACTG GGCAAAATTG GGGTGTTTCA GCACATCATT 
CGGAGAGAGA TAAAAGATGA GGAAGCTCGA CACCTCTCAC GGAAATTTAA AGACTGGGCT 
TATGGACCAG TGTACTCCAA TCTGTATGAT CTGTCCTCCC TGGACACCTG CGGAGAGGAA 
GTGTCTGTTC TGGAAATACT CGTCTACAAC AGCAAGATTG AGAATCGGCA TGAGATGTTG 
GCGGTGGAAC CAATAAATGA ACTGCTGAGG GCCAAGTGGC AGAAATTTGC AGCGGTGACC 
TTCTACATCA GTGTGTTTTC CTATCTTGTG ACTATGATTA TCTTCACTCT TGTGGCCTAC 
TATCGCCCAT CAGTGGGAAA ACCCCCTTAT GCTTATGATA CCACAGAAGA CAAGGTGCGT 
CTGGCTGGAG AGATCATCAC AGTGGGTTCT GGACTCTTTT TCTTTGTAAC AAATCTTCAT 
CTACTCTGTG TTGGTGCTGG TGAGCGCAGC GCTGTATCTG TCAGGCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299582.1
CDS	387..2057
Translation	MTDQGFSASTLLKRYRLAMTESLSVSSPPDNSAQDSSEAADGDPNFPMSSMAALLENDDVSQPTHELPRPGQQNDQKQNMRIRFPGPFKKGVPNPMDLLESDYTEYPKQAPMDSMFDYGTCRQINNNKKGRRKKLPRGKAEIGMSCDEGSPEPPVLKVFNRWMLFEAVSRADPRALDGLLQYLQSHEKRLTDEEFKELSTGKTCLPKALLNLHNGQNDTIPILVDIAEQTGNLREFINTPFRDVYYRGQMALHIAIERRCKQYVELLVEKGADVHAQARGRFFQPRDEGGYFYFGELPLSLAACTNQPDMVHYLTENGHKKADLRRQDSRGNTVLHALVHIADNTRDNTRFVTKMFDLLLIKCAKLYPDCNLENILNNDGMSPLMMAAKLGKIGVFQHIIRREIKDEEARHLSRKFKDWAYGPVYSNLYDLSSLDTCGEEVSVLEILVYNSKIENRHEMLAVEPINELLRAKWQKFAAVTFYISVFSYLVTMIIFTLVAYYRPSVGKPPYAYDTTEDKVRLAGEIITVGSGLFFFVTNLHLLCVGAGERSAVSVRH

Target ORF information:

RefSeq Version	XM_009301307.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301307.2

ORF Insert Sequence:

ATGACTGACC AGGGTTTTTC TGCTTCCACT CTACTGAAGC GCTATCGTCT GGCCATGACA 
GAGTCCTTGT CTGTCTCCTC ACCACCAGAC AATTCAGCTC AGGACTCTTC AGAGGCAGCA 
GATGGAGATC CCAACTTCCC CATGTCTTCA ATGGCAGCGC TACTGGAAAA TGATGATGTA 
TCACAGCCAA CTCATGAATT GCCTCGACCA GGACAACAGA ACGACCAGAA GCAGAACATG 
CGGATTAGGT TCCCAGGACC CTTTAAAAAG GGGGTTCCTA ACCCTATGGA TCTTCTTGAG 
TCGGATTATA CAGAGTATCC TAAACAAGCA CCTATGGATT CCATGTTTGA CTACGGCACA 
TGCAGGCAGA TCAACAACAA TAAGAAAGGA AGACGGAAGA AGCTTCCACG AGGGAAGGCA 
GAGATCGGGA TGTCATGTGA TGAGGGATCC CCGGAGCCCC CTGTTCTCAA AGTCTTCAAC 
CGCTGGATGT TGTTCGAAGC TGTATCTCGA GCAGATCCAA GAGCTCTGGA CGGCCTTCTG 
CAGTATCTTC AGTCCCACGA AAAGAGGCTG ACCGATGAAG AGTTCAAAGA ACTTTCTACA 
GGGAAAACCT GCCTACCAAA AGCACTTCTC AACCTGCACA ATGGTCAAAA CGACACCATC 
CCAATATTGG TGGACATTGC TGAACAGACA GGAAACCTCA GAGAGTTCAT CAACACACCC 
TTTAGAGATG TCTATTATAG GGGTCAGATG GCTCTGCATA TTGCTATAGA GCGACGATGC 
AAACAGTATG TGGAGCTTCT GGTGGAGAAG GGGGCTGATG TCCATGCGCA GGCCAGGGGA 
CGCTTCTTCC AGCCCAGAGA TGAGGGTGGA TACTTCTACT TTGGTGAACT GCCCCTCTCA 
CTCGCAGCTT GTACCAACCA ACCAGACATG GTGCATTACC TGACAGAGAA TGGACACAAG 
AAGGCGGATC TGAGGAGGCA GGACTCAAGG GGAAACACAG TCCTGCATGC TTTGGTCCAC 
ATTGCTGACA ACACTCGGGA CAACACACGT TTTGTCACTA AGATGTTTGA CTTGCTGCTC 
ATTAAATGTG CCAAACTCTA CCCGGATTGC AACCTGGAGA ATATACTCAA CAACGACGGC 
ATGTCCCCTC TCATGATGGC CGCCAAACTG GGCAAAATTG GGGTGTTTCA GCACATCATT 
CGGAGAGAGA TAAAAGATGA GGAAGCTCGA CACCTCTCAC GGAAATTTAA AGACTGGGCT 
TATGGACCAG TGTACTCCAA TCTGTATGAT CTGTCCTCCC TGGACACCTG CGGAGAGGAA 
GTGTCTGTTC TGGAAATACT CGTCTACAAC AGCAAGATTG AGAATCGGCA TGAGATGTTG 
GCGGTGGAAC CAATAAATGA ACTGCTGAGG GCCAAGTGGC AGAAATTTGC AGCGGTGACC 
TTCTACATCA GTGTGTTTTC CTATCTTGTG ACTATGATTA TCTTCACTCT TGTGGCCTAC 
TATCGCCCAT CAGTGGGAAA ACCCCCTTAT GCTTATGATA CCACAGAAGA CAAGGTGCGT 
CTGGCTGGAG AGATCATCAC AGTGGGTTCT GGACTCTTTT TCTTTGTAAC AAATCTTCAT 
CTACTCTGTG TTGGTGCTGG TGAGCGCAGC GCTGTATCTG TCAGGCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpv4 ( NM_001042730.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001036195.1 Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), mRNA.

Price & Availability↑

CloneID	ODa04267
Clone ID Related Accession (Same CDS sequence)	NM_001042730.1
Accession Version	NM_001042730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily V member 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ858167.1. On Aug 16, 2006 this sequence version replaced XM_680995.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ858167.1, BC163540.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACAGAGT CCTTGTCTGT CTCCTCACCA CCAGACAATT CAGCTCAGGA CTCTTCAGAG 
GCAGCAGATG GAGATCCCAA CTTCCCCATG TCTTCAATGG CAGCGCTACT GGAAAATGAT 
GATGTATCAC AGCCAACTCA TGAATTGCCT CGACCAGGAC AACAGAACGA CCAGAAGCAG 
AACATGCGGA TTAGGTTCCC AGGACCCTTT AAAAAGGGGG TTCCTAACCC TATGGATCTT 
CTTGAGTCGG ATTATACAGA GTATCCTAAA CAAGCACCTA TGGATTCCAT GTTTGACTAC 
GGCACATGCA GGCAGATCAA CAACAATAAG AAAGGAAGAC GGAAGAAGCT TCCACGAGGG 
AAGGCAGAGA TCGGGATGTC ATGTGATGAG GGATCCCCGG AGCCCCCTGT TCTCAAAGTC 
TTCAACCGCT GGATGTTGTT CGAAGCTGTA TCTCGAGCAG ATCCAAGAGC TCTGGACGGC 
CTTCTGCAGT ATCTTCAGTC CCACGAAAAG AGGCTGACCG ATGAAGAGTT CAAAGAACTT 
TCTACAGGGA AAACCTGCCT ACCAAAAGCA CTTCTCAACC TGCACAATGG TCAAAACGAC 
ACCATCCCAA TATTGGTGGA CATTGCTGAA CAGACAGGAA ACCTCAGAGA GTTCATCAAC 
ACACCCTTTA GAGATGTCTA TTATAGGGGT CAGATGGCTC TGCATATTGC TATAGAGCGA 
CGATGCAAAC AGTATGTGGA GCTTCTGGTG GAGAAGGGGG CTGATGTCCA TGCGCAGGCC 
AGGGGACGCT TCTTCCAGCC CAGAGATGAG GGTGGATACT TCTACTTTGG TGAACTGCCC 
CTCTCACTCG CAGCTTGTAC CAACCAACCA GACATGGTGC ATTACCTGAC AGAGAATGGA 
CACAAGAAGG CGGATCTGAG GAGGCAGGAC TCAAGGGGAA ACACAGTCCT GCATGCTTTG 
GTCCACATTG CTGACAACAC TCGGGACAAC ACACGTTTTG TCACTAAGAT GTTTGACTTG 
CTGCTCATTA AATGTGCCAA ACTCTACCCG GATTGCAACC TGGAGAATAT ACTCAACAAC 
GACGGCATGT CCCCTCTCAT GATGGCCGCC AAACTGGGCA AAATTGGGGT GTTTCAGCAC 
ACCATTCGGA GAGAGATAAA AGATGAGGAA GCTCGACACC TCTCACGGAA ATTTAAAGAC 
TGGGCTTATG GACCAGTGTA CTCCAATCTG TATGATCTGT CCTCCCTGGA CACCTGCGGA 
GAGGAAGTGT CTGTTCTGGA AATACTCGTC TACAACAGCA AGATTGAGAA TCGGCATGAG 
ATGTTGGCGG TGGAACCAAT AAATGAACTG CTGAGGGCCA AGTGGCAGAA ATTTGCAGCG 
GTGACCTTCT ACATCAGTGT GTTTTCCTAT CTTGTGACTA TGATTATCTT CACTCTTGTG 
GCCTACTATC GCCCATCAGT GGGAAAACCC CCTTATGCTT ATGATACCAC AGAAGACAAG 
GTGCGTCTGG GTGGAGAGAT CATCACAGTG GGTTCTGGAC TCTTTTTCTT TGTAACAAAT 
ATTAAGGACC TTTTCCTGAA GAAGTGCCCC GGGGTGAATT CTATATTTGT TGATGGATCC 
TTTCAGCTTC TCTACTTCAT CTACTCTGTG TTGGTGGTGG GGAGCGCAGC GCTGTATCTG 
TCAGGCATTG AGGCCTACGT GTCTGTGATG GTGTTTGCTT TAACACTAGG GGGGATGAAC 
CCCCTCTATT TTACCAGAGG CCTTAAACTC ACTGGAACTT ACAGCATCAT GATCCAAAAG 
ATTCTCATCA AAGATTTGTT CCGGTTCCTG CTGGTCTACG TGCTCTTCAT GATTGGCTAT 
GCTTCAGCAT TAGTGTCGCT GTTGACCATC TGCCCTAATA AGGACACGTG TAAGGAAAAC 
TGTCCTACAT ATCCCGAATG TCGAGACACA AACACCTTCA GTGAATTCTT GCTGGACCTC 
TTCAAGCTGA CCATAGGCAT CGGAGACCTG GACAACATGC TGAAAGGCGC ACAGTATCCC 
GCCGTCTTCC TCATTCTGCT GGTCACCTAC ATTATCCTCA CCTTCGTCCC GTTGCTCAAC 
ATGTTAATCG CTCTGATGGG GGAGACGGTG GGACAGGTGT CCAAAGAAAG CAAGAAGATC 
TGGAAATTGC AGTGGGCGAC TACTATCCTG GACATTGAGC GTTCTTTCCC TGTGTGTCTG 
CGGCGGTCTT TCCGTGTGGG AGAGATGGTC ACTGTGGGCA AGGGTTTGGA CGGAAAGCCA 
GACAAACGCT GGTGCTTCAG GGTGGATGAG GTGAAGTGGT CCCACTGGAA TCAAAATCTG 
GGCATCATCA ATGAGGATCC AGGCCAGAAA GACCTTTCTG AACACACACA GGGTGGACGG 
GGCCTTCGAA GAGACCGCTG GTCCACTGTA GTACCGAGAG TGGTTGAACT GAATCGCGGC 
TCCAGAGATC ACACCGTAGA GATGGAGCCT CTCACAGGGA GACACCGACT CAAGTCTGAA 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036195.1
CDS	11..2536
Translation	MTESLSVSSPPDNSAQDSSEAADGDPNFPMSSMAALLENDDVSQPTHELPRPGQQNDQKQNMRIRFPGPFKKGVPNPMDLLESDYTEYPKQAPMDSMFDYGTCRQINNNKKGRRKKLPRGKAEIGMSCDEGSPEPPVLKVFNRWMLFEAVSRADPRALDGLLQYLQSHEKRLTDEEFKELSTGKTCLPKALLNLHNGQNDTIPILVDIAEQTGNLREFINTPFRDVYYRGQMALHIAIERRCKQYVELLVEKGADVHAQARGRFFQPRDEGGYFYFGELPLSLAACTNQPDMVHYLTENGHKKADLRRQDSRGNTVLHALVHIADNTRDNTRFVTKMFDLLLIKCAKLYPDCNLENILNNDGMSPLMMAAKLGKIGVFQHTIRREIKDEEARHLSRKFKDWAYGPVYSNLYDLSSLDTCGEEVSVLEILVYNSKIENRHEMLAVEPINELLRAKWQKFAAVTFYISVFSYLVTMIIFTLVAYYRPSVGKPPYAYDTTEDKVRLGGEIITVGSGLFFFVTNIKDLFLKKCPGVNSIFVDGSFQLLYFIYSVLVVGSAALYLSGIEAYVSVMVFALTLGGMNPLYFTRGLKLTGTYSIMIQKILIKDLFRFLLVYVLFMIGYASALVSLLTICPNKDTCKENCPTYPECRDTNTFSEFLLDLFKLTIGIGDLDNMLKGAQYPAVFLILLVTYIILTFVPLLNMLIALMGETVGQVSKESKKIWKLQWATTILDIERSFPVCLRRSFRVGEMVTVGKGLDGKPDKRWCFRVDEVKWSHWNQNLGIINEDPGQKDLSEHTQGGRGLRRDRWSTVVPRVVELNRGSRDHTVEMEPLTGRHRLKSES

Target ORF information:

RefSeq Version	NM_001042730.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042730.1

ORF Insert Sequence:

ATGACAGAGT CCTTGTCTGT CTCCTCACCA CCAGACAATT CAGCTCAGGA CTCTTCAGAG 
GCAGCAGATG GAGATCCCAA CTTCCCCATG TCTTCAATGG CAGCGCTACT GGAAAATGAT 
GATGTATCAC AGCCAACTCA TGAATTGCCT CGACCAGGAC AACAGAACGA CCAGAAGCAG 
AACATGCGGA TTAGGTTCCC AGGACCCTTT AAAAAGGGGG TTCCTAACCC TATGGATCTT 
CTTGAGTCGG ATTATACAGA GTATCCTAAA CAAGCACCTA TGGATTCCAT GTTTGACTAC 
GGCACATGCA GGCAGATCAA CAACAATAAG AAAGGAAGAC GGAAGAAGCT TCCACGAGGG 
AAGGCAGAGA TCGGGATGTC ATGTGATGAG GGATCCCCGG AGCCCCCTGT TCTCAAAGTC 
TTCAACCGCT GGATGTTGTT CGAAGCTGTA TCTCGAGCAG ATCCAAGAGC TCTGGACGGC 
CTTCTGCAGT ATCTTCAGTC CCACGAAAAG AGGCTGACCG ATGAAGAGTT CAAAGAACTT 
TCTACAGGGA AAACCTGCCT ACCAAAAGCA CTTCTCAACC TGCACAATGG TCAAAACGAC 
ACCATCCCAA TATTGGTGGA CATTGCTGAA CAGACAGGAA ACCTCAGAGA GTTCATCAAC 
ACACCCTTTA GAGATGTCTA TTATAGGGGT CAGATGGCTC TGCATATTGC TATAGAGCGA 
CGATGCAAAC AGTATGTGGA GCTTCTGGTG GAGAAGGGGG CTGATGTCCA TGCGCAGGCC 
AGGGGACGCT TCTTCCAGCC CAGAGATGAG GGTGGATACT TCTACTTTGG TGAACTGCCC 
CTCTCACTCG CAGCTTGTAC CAACCAACCA GACATGGTGC ATTACCTGAC AGAGAATGGA 
CACAAGAAGG CGGATCTGAG GAGGCAGGAC TCAAGGGGAA ACACAGTCCT GCATGCTTTG 
GTCCACATTG CTGACAACAC TCGGGACAAC ACACGTTTTG TCACTAAGAT GTTTGACTTG 
CTGCTCATTA AATGTGCCAA ACTCTACCCG GATTGCAACC TGGAGAATAT ACTCAACAAC 
GACGGCATGT CCCCTCTCAT GATGGCCGCC AAACTGGGCA AAATTGGGGT GTTTCAGCAC 
ACCATTCGGA GAGAGATAAA AGATGAGGAA GCTCGACACC TCTCACGGAA ATTTAAAGAC 
TGGGCTTATG GACCAGTGTA CTCCAATCTG TATGATCTGT CCTCCCTGGA CACCTGCGGA 
GAGGAAGTGT CTGTTCTGGA AATACTCGTC TACAACAGCA AGATTGAGAA TCGGCATGAG 
ATGTTGGCGG TGGAACCAAT AAATGAACTG CTGAGGGCCA AGTGGCAGAA ATTTGCAGCG 
GTGACCTTCT ACATCAGTGT GTTTTCCTAT CTTGTGACTA TGATTATCTT CACTCTTGTG 
GCCTACTATC GCCCATCAGT GGGAAAACCC CCTTATGCTT ATGATACCAC AGAAGACAAG 
GTGCGTCTGG GTGGAGAGAT CATCACAGTG GGTTCTGGAC TCTTTTTCTT TGTAACAAAT 
ATTAAGGACC TTTTCCTGAA GAAGTGCCCC GGGGTGAATT CTATATTTGT TGATGGATCC 
TTTCAGCTTC TCTACTTCAT CTACTCTGTG TTGGTGGTGG GGAGCGCAGC GCTGTATCTG 
TCAGGCATTG AGGCCTACGT GTCTGTGATG GTGTTTGCTT TAACACTAGG GGGGATGAAC 
CCCCTCTATT TTACCAGAGG CCTTAAACTC ACTGGAACTT ACAGCATCAT GATCCAAAAG 
ATTCTCATCA AAGATTTGTT CCGGTTCCTG CTGGTCTACG TGCTCTTCAT GATTGGCTAT 
GCTTCAGCAT TAGTGTCGCT GTTGACCATC TGCCCTAATA AGGACACGTG TAAGGAAAAC 
TGTCCTACAT ATCCCGAATG TCGAGACACA AACACCTTCA GTGAATTCTT GCTGGACCTC 
TTCAAGCTGA CCATAGGCAT CGGAGACCTG GACAACATGC TGAAAGGCGC ACAGTATCCC 
GCCGTCTTCC TCATTCTGCT GGTCACCTAC ATTATCCTCA CCTTCGTCCC GTTGCTCAAC 
ATGTTAATCG CTCTGATGGG GGAGACGGTG GGACAGGTGT CCAAAGAAAG CAAGAAGATC 
TGGAAATTGC AGTGGGCGAC TACTATCCTG GACATTGAGC GTTCTTTCCC TGTGTGTCTG 
CGGCGGTCTT TCCGTGTGGG AGAGATGGTC ACTGTGGGCA AGGGTTTGGA CGGAAAGCCA 
GACAAACGCT GGTGCTTCAG GGTGGATGAG GTGAAGTGGT CCCACTGGAA TCAAAATCTG 
GGCATCATCA ATGAGGATCC AGGCCAGAAA GACCTTTCTG AACACACACA GGGTGGACGG 
GGCCTTCGAA GAGACCGCTG GTCCACTGTA GTACCGAGAG TGGTTGAACT GAATCGCGGC 
TCCAGAGATC ACACCGTAGA GATGGAGCCT CTCACAGGGA GACACCGACT CAAGTCTGAA 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpv4 ( XM_005165151.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165208.1 Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10274
Clone ID Related Accession (Same CDS sequence)	XM_005165151.4 , XM_005165151.3
Accession Version	XM_005165151.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily V member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165151.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGACC AGGGTTTTTC TGCTTCCACT CTACTGAAGC GCTATCGTCT GGCCATGACA 
GAGTCCTTGT CTGTCTCCTC ACCACCAGAC AATTCAGCTC AGGACTCTTC AGAGGCAGCA 
GATGGAGATC CCAACTTCCC CATGTCTTCA ATGGCAGCGC TACTGGAAAA TGATGATGTA 
TCACAGCCAA CTCATGAATT GCCTCGACCA GGACAACAGA ACGACCAGAA GCAGAACATG 
CGGATTAGGT TCCCAGGACC CTTTAAAAAG GGGGTTCCTA ACCCTATGGA TCTTCTTGAG 
TCGGATTATA CAGAGTATCC TAAACAAGCA CCTATGGATT CCATGTTTGA CTACGGCACA 
TGCAGGCAGA TCAACAACAA TAAGAAAGGA AGACGGAAGA AGCTTCCACG AGGGAAGGCA 
GAGATCGGGA TGTCATGTGA TGAGGGATCC CCGGAGCCCC CTGTTCTCAA AGTCTTCAAC 
CGCTGGATGT TGTTCGAAGC TGTATCTCGA GCAGATCCAA GAGCTCTGGA CGGCCTTCTG 
CAGTATCTTC AGTCCCACGA AAAGAGGCTG ACCGATGAAG AGTTCAAAGA ACTTTCTACA 
GGGAAAACCT GCCTACCAAA AGCACTTCTC AACCTGCACA ATGGTCAAAA CGACACCATC 
CCAATATTGG TGGACATTGC TGAACAGACA GGAAACCTCA GAGAGTTCAT CAACACACCC 
TTTAGAGATG TCTATTATAG GGGTCAGATG GCTCTGCATA TTGCTATAGA GCGACGATGC 
AAACAGTATG TGGAGCTTCT GGTGGAGAAG GGGGCTGATG TCCATGCGCA GGCCAGGGGA 
CGCTTCTTCC AGCCCAGAGA TGAGGGTGGA TACTTCTACT TTGGTGAACT GCCCCTCTCA 
CTCGCAGCTT GTACCAACCA ACCAGACATG GTGCATTACC TGACAGAGAA TGGACACAAG 
AAGGCGGATC TGAGGAGGCA GGACTCAAGG GGAAACACAG TCCTGCATGC TTTGGTCCAC 
ATTGCTGACA ACACTCGGGA CAACACACGT TTTGTCACTA AGATGTTTGA CTTGCTGCTC 
ATTAAATGTG CCAAACTCTA CCCGGATTGC AACCTGGAGA ATATACTCAA CAACGACGGC 
ATGTCCCCTC TCATGATGGC CGCCAAACTG GGCAAAATTG GGGTGTTTCA GCACATCATT 
CGGAGAGAGA TAAAAGATGA GGAAGCTCGA CACCTCTCAC GGAAATTTAA AGACTGGGCT 
TATGGACCAG TGTACTCCAA TCTGTATGAT CTGTCCTCCC TGGACACCTG CGGAGAGGAA 
GTGTCTGTTC TGGAAATACT CGTCTACAAC AGCAAGATTG AGAATCGGCA TGAGATGTTG 
GCGGTGGAAC CAATAAATGA ACTGCTGAGG GCCAAGTGGC AGAAATTTGC AGCGGTGACC 
TTCTACATCA GTGTGTTTTC CTATCTTGTG ACTATGATTA TCTTCACTCT TGTGGCCTAC 
TATCGCCCAT CAGTGGGAAA ACCCCCTTAT GCTTATGATA CCACAGAAGA CAAGGTGCGT 
CTGGCTGGAG AGATCATCAC AGTGGGTTCT GGACTCTTTT TCTTTGTAAC AAATATTAAG 
GACCTTTTCC TGAAGAAGTG CCCCGGGGTG AATTCTATAT TTGTTGATGG ATCCTTTCAG 
CTTCTCTACT TCATCTACTC TGTGTTGGTG CTGGTGAGCG CAGCGCTGTA TCTGTCAGGC 
ATTGAGGCCT ACGTGTCTGT GATGGTGTTT GCTTTAACAC TAGGCTGGAT GAACACCCTC 
TATTTTACCA GAGGCCTTAA ACTCACTGGA ACTTACAGCA TCATGATCCA AAAGATTCTC 
ATCAAAGATT TGTTCCGGTT CCTGCTGGTC TACGTGCTCT TCATGATTGG TTATGCTTCA 
GCATTAGTGT CGCTGTTGAC CATCTGCCCT GATAAGGACA CGTGTAAGGA AAACTGTCCT 
ACATATCCCG AATGTCGAGA CACAAACACC TTCAGTGAAT TCTTGCTGGA CCTCTTCAAG 
CTGACCATAG GCATCGGAGA CCTGGACAAC ATGCTGAAAG GCGCACAGTA TCCCGCCGTC 
TTCCTCATTC TGCTGGTCAC CTACATTATC CTCACCTTCG TCCTGTTGCT CAACATGTTA 
ATCGCTCTGA TGGGGGAGAC GGTGGGACAG GTGTCCAAAG AAAGCAAGAA GATCTGGAAA 
TTGCAGTGGG CGACTACTAT CCTGGACATT GAGCGTTCTT TCCCTGTGTG TCTGCGGCGG 
TCTTTCCGTG TGGGAGAGAT GGTCACTGTG GGCAAGGGTT TGGATGGAAA GCCAGACAAA 
CGCTGGTGCT TCAGGGTGGA CGAGGTGAAG TGGTCCCACT GGAATCAAAA TCTGGGCATC 
ATCAATGAGG ATCCAGGCCA GAAAGACCTT TCTGAACACA CACAGGGTGG ACGGGGCCTT 
CGAAGAGACC GCTGGTCCAC TGTAGTACCG AGAGTGGTTG AACTGAATCG CGGCTCCAGA 
GATCACACCG TAGAGATGGA GCCTCTCACA GGGAGACACC GACTCAAGTC TGAAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165208.1
CDS	400..2979
Translation	MTDQGFSASTLLKRYRLAMTESLSVSSPPDNSAQDSSEAADGDPNFPMSSMAALLENDDVSQPTHELPRPGQQNDQKQNMRIRFPGPFKKGVPNPMDLLESDYTEYPKQAPMDSMFDYGTCRQINNNKKGRRKKLPRGKAEIGMSCDEGSPEPPVLKVFNRWMLFEAVSRADPRALDGLLQYLQSHEKRLTDEEFKELSTGKTCLPKALLNLHNGQNDTIPILVDIAEQTGNLREFINTPFRDVYYRGQMALHIAIERRCKQYVELLVEKGADVHAQARGRFFQPRDEGGYFYFGELPLSLAACTNQPDMVHYLTENGHKKADLRRQDSRGNTVLHALVHIADNTRDNTRFVTKMFDLLLIKCAKLYPDCNLENILNNDGMSPLMMAAKLGKIGVFQHIIRREIKDEEARHLSRKFKDWAYGPVYSNLYDLSSLDTCGEEVSVLEILVYNSKIENRHEMLAVEPINELLRAKWQKFAAVTFYISVFSYLVTMIIFTLVAYYRPSVGKPPYAYDTTEDKVRLAGEIITVGSGLFFFVTNIKDLFLKKCPGVNSIFVDGSFQLLYFIYSVLVLVSAALYLSGIEAYVSVMVFALTLGWMNTLYFTRGLKLTGTYSIMIQKILIKDLFRFLLVYVLFMIGYASALVSLLTICPDKDTCKENCPTYPECRDTNTFSEFLLDLFKLTIGIGDLDNMLKGAQYPAVFLILLVTYIILTFVLLLNMLIALMGETVGQVSKESKKIWKLQWATTILDIERSFPVCLRRSFRVGEMVTVGKGLDGKPDKRWCFRVDEVKWSHWNQNLGIINEDPGQKDLSEHTQGGRGLRRDRWSTVVPRVVELNRGSRDHTVEMEPLTGRHRLKSES

Target ORF information:

RefSeq Version	XM_005165151.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165151.4

ORF Insert Sequence:

ATGACTGACC AGGGTTTTTC TGCTTCCACT CTACTGAAGC GCTATCGTCT GGCCATGACA 
GAGTCCTTGT CTGTCTCCTC ACCACCAGAC AATTCAGCTC AGGACTCTTC AGAGGCAGCA 
GATGGAGATC CCAACTTCCC CATGTCTTCA ATGGCAGCGC TACTGGAAAA TGATGATGTA 
TCACAGCCAA CTCATGAATT GCCTCGACCA GGACAACAGA ACGACCAGAA GCAGAACATG 
CGGATTAGGT TCCCAGGACC CTTTAAAAAG GGGGTTCCTA ACCCTATGGA TCTTCTTGAG 
TCGGATTATA CAGAGTATCC TAAACAAGCA CCTATGGATT CCATGTTTGA CTACGGCACA 
TGCAGGCAGA TCAACAACAA TAAGAAAGGA AGACGGAAGA AGCTTCCACG AGGGAAGGCA 
GAGATCGGGA TGTCATGTGA TGAGGGATCC CCGGAGCCCC CTGTTCTCAA AGTCTTCAAC 
CGCTGGATGT TGTTCGAAGC TGTATCTCGA GCAGATCCAA GAGCTCTGGA CGGCCTTCTG 
CAGTATCTTC AGTCCCACGA AAAGAGGCTG ACCGATGAAG AGTTCAAAGA ACTTTCTACA 
GGGAAAACCT GCCTACCAAA AGCACTTCTC AACCTGCACA ATGGTCAAAA CGACACCATC 
CCAATATTGG TGGACATTGC TGAACAGACA GGAAACCTCA GAGAGTTCAT CAACACACCC 
TTTAGAGATG TCTATTATAG GGGTCAGATG GCTCTGCATA TTGCTATAGA GCGACGATGC 
AAACAGTATG TGGAGCTTCT GGTGGAGAAG GGGGCTGATG TCCATGCGCA GGCCAGGGGA 
CGCTTCTTCC AGCCCAGAGA TGAGGGTGGA TACTTCTACT TTGGTGAACT GCCCCTCTCA 
CTCGCAGCTT GTACCAACCA ACCAGACATG GTGCATTACC TGACAGAGAA TGGACACAAG 
AAGGCGGATC TGAGGAGGCA GGACTCAAGG GGAAACACAG TCCTGCATGC TTTGGTCCAC 
ATTGCTGACA ACACTCGGGA CAACACACGT TTTGTCACTA AGATGTTTGA CTTGCTGCTC 
ATTAAATGTG CCAAACTCTA CCCGGATTGC AACCTGGAGA ATATACTCAA CAACGACGGC 
ATGTCCCCTC TCATGATGGC CGCCAAACTG GGCAAAATTG GGGTGTTTCA GCACATCATT 
CGGAGAGAGA TAAAAGATGA GGAAGCTCGA CACCTCTCAC GGAAATTTAA AGACTGGGCT 
TATGGACCAG TGTACTCCAA TCTGTATGAT CTGTCCTCCC TGGACACCTG CGGAGAGGAA 
GTGTCTGTTC TGGAAATACT CGTCTACAAC AGCAAGATTG AGAATCGGCA TGAGATGTTG 
GCGGTGGAAC CAATAAATGA ACTGCTGAGG GCCAAGTGGC AGAAATTTGC AGCGGTGACC 
TTCTACATCA GTGTGTTTTC CTATCTTGTG ACTATGATTA TCTTCACTCT TGTGGCCTAC 
TATCGCCCAT CAGTGGGAAA ACCCCCTTAT GCTTATGATA CCACAGAAGA CAAGGTGCGT 
CTGGCTGGAG AGATCATCAC AGTGGGTTCT GGACTCTTTT TCTTTGTAAC AAATATTAAG 
GACCTTTTCC TGAAGAAGTG CCCCGGGGTG AATTCTATAT TTGTTGATGG ATCCTTTCAG 
CTTCTCTACT TCATCTACTC TGTGTTGGTG CTGGTGAGCG CAGCGCTGTA TCTGTCAGGC 
ATTGAGGCCT ACGTGTCTGT GATGGTGTTT GCTTTAACAC TAGGCTGGAT GAACACCCTC 
TATTTTACCA GAGGCCTTAA ACTCACTGGA ACTTACAGCA TCATGATCCA AAAGATTCTC 
ATCAAAGATT TGTTCCGGTT CCTGCTGGTC TACGTGCTCT TCATGATTGG TTATGCTTCA 
GCATTAGTGT CGCTGTTGAC CATCTGCCCT GATAAGGACA CGTGTAAGGA AAACTGTCCT 
ACATATCCCG AATGTCGAGA CACAAACACC TTCAGTGAAT TCTTGCTGGA CCTCTTCAAG 
CTGACCATAG GCATCGGAGA CCTGGACAAC ATGCTGAAAG GCGCACAGTA TCCCGCCGTC 
TTCCTCATTC TGCTGGTCAC CTACATTATC CTCACCTTCG TCCTGTTGCT CAACATGTTA 
ATCGCTCTGA TGGGGGAGAC GGTGGGACAG GTGTCCAAAG AAAGCAAGAA GATCTGGAAA 
TTGCAGTGGG CGACTACTAT CCTGGACATT GAGCGTTCTT TCCCTGTGTG TCTGCGGCGG 
TCTTTCCGTG TGGGAGAGAT GGTCACTGTG GGCAAGGGTT TGGATGGAAA GCCAGACAAA 
CGCTGGTGCT TCAGGGTGGA CGAGGTGAAG TGGTCCCACT GGAATCAAAA TCTGGGCATC 
ATCAATGAGG ATCCAGGCCA GAAAGACCTT TCTGAACACA CACAGGGTGG ACGGGGCCTT 
CGAAGAGACC GCTGGTCCAC TGTAGTACCG AGAGTGGTTG AACTGAATCG CGGCTCCAGA 
GATCACACCG TAGAGATGGA GCCTCTCACA GGGAGACACC GACTCAAGTC TGAAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpv4 ( XM_005165151.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165208.1 Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10274
Clone ID Related Accession (Same CDS sequence)	XM_005165151.4 , XM_005165151.3
Accession Version	XM_005165151.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily V member 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165151.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGACC AGGGTTTTTC TGCTTCCACT CTACTGAAGC GCTATCGTCT GGCCATGACA 
GAGTCCTTGT CTGTCTCCTC ACCACCAGAC AATTCAGCTC AGGACTCTTC AGAGGCAGCA 
GATGGAGATC CCAACTTCCC CATGTCTTCA ATGGCAGCGC TACTGGAAAA TGATGATGTA 
TCACAGCCAA CTCATGAATT GCCTCGACCA GGACAACAGA ACGACCAGAA GCAGAACATG 
CGGATTAGGT TCCCAGGACC CTTTAAAAAG GGGGTTCCTA ACCCTATGGA TCTTCTTGAG 
TCGGATTATA CAGAGTATCC TAAACAAGCA CCTATGGATT CCATGTTTGA CTACGGCACA 
TGCAGGCAGA TCAACAACAA TAAGAAAGGA AGACGGAAGA AGCTTCCACG AGGGAAGGCA 
GAGATCGGGA TGTCATGTGA TGAGGGATCC CCGGAGCCCC CTGTTCTCAA AGTCTTCAAC 
CGCTGGATGT TGTTCGAAGC TGTATCTCGA GCAGATCCAA GAGCTCTGGA CGGCCTTCTG 
CAGTATCTTC AGTCCCACGA AAAGAGGCTG ACCGATGAAG AGTTCAAAGA ACTTTCTACA 
GGGAAAACCT GCCTACCAAA AGCACTTCTC AACCTGCACA ATGGTCAAAA CGACACCATC 
CCAATATTGG TGGACATTGC TGAACAGACA GGAAACCTCA GAGAGTTCAT CAACACACCC 
TTTAGAGATG TCTATTATAG GGGTCAGATG GCTCTGCATA TTGCTATAGA GCGACGATGC 
AAACAGTATG TGGAGCTTCT GGTGGAGAAG GGGGCTGATG TCCATGCGCA GGCCAGGGGA 
CGCTTCTTCC AGCCCAGAGA TGAGGGTGGA TACTTCTACT TTGGTGAACT GCCCCTCTCA 
CTCGCAGCTT GTACCAACCA ACCAGACATG GTGCATTACC TGACAGAGAA TGGACACAAG 
AAGGCGGATC TGAGGAGGCA GGACTCAAGG GGAAACACAG TCCTGCATGC TTTGGTCCAC 
ATTGCTGACA ACACTCGGGA CAACACACGT TTTGTCACTA AGATGTTTGA CTTGCTGCTC 
ATTAAATGTG CCAAACTCTA CCCGGATTGC AACCTGGAGA ATATACTCAA CAACGACGGC 
ATGTCCCCTC TCATGATGGC CGCCAAACTG GGCAAAATTG GGGTGTTTCA GCACATCATT 
CGGAGAGAGA TAAAAGATGA GGAAGCTCGA CACCTCTCAC GGAAATTTAA AGACTGGGCT 
TATGGACCAG TGTACTCCAA TCTGTATGAT CTGTCCTCCC TGGACACCTG CGGAGAGGAA 
GTGTCTGTTC TGGAAATACT CGTCTACAAC AGCAAGATTG AGAATCGGCA TGAGATGTTG 
GCGGTGGAAC CAATAAATGA ACTGCTGAGG GCCAAGTGGC AGAAATTTGC AGCGGTGACC 
TTCTACATCA GTGTGTTTTC CTATCTTGTG ACTATGATTA TCTTCACTCT TGTGGCCTAC 
TATCGCCCAT CAGTGGGAAA ACCCCCTTAT GCTTATGATA CCACAGAAGA CAAGGTGCGT 
CTGGCTGGAG AGATCATCAC AGTGGGTTCT GGACTCTTTT TCTTTGTAAC AAATATTAAG 
GACCTTTTCC TGAAGAAGTG CCCCGGGGTG AATTCTATAT TTGTTGATGG ATCCTTTCAG 
CTTCTCTACT TCATCTACTC TGTGTTGGTG CTGGTGAGCG CAGCGCTGTA TCTGTCAGGC 
ATTGAGGCCT ACGTGTCTGT GATGGTGTTT GCTTTAACAC TAGGCTGGAT GAACACCCTC 
TATTTTACCA GAGGCCTTAA ACTCACTGGA ACTTACAGCA TCATGATCCA AAAGATTCTC 
ATCAAAGATT TGTTCCGGTT CCTGCTGGTC TACGTGCTCT TCATGATTGG TTATGCTTCA 
GCATTAGTGT CGCTGTTGAC CATCTGCCCT GATAAGGACA CGTGTAAGGA AAACTGTCCT 
ACATATCCCG AATGTCGAGA CACAAACACC TTCAGTGAAT TCTTGCTGGA CCTCTTCAAG 
CTGACCATAG GCATCGGAGA CCTGGACAAC ATGCTGAAAG GCGCACAGTA TCCCGCCGTC 
TTCCTCATTC TGCTGGTCAC CTACATTATC CTCACCTTCG TCCTGTTGCT CAACATGTTA 
ATCGCTCTGA TGGGGGAGAC GGTGGGACAG GTGTCCAAAG AAAGCAAGAA GATCTGGAAA 
TTGCAGTGGG CGACTACTAT CCTGGACATT GAGCGTTCTT TCCCTGTGTG TCTGCGGCGG 
TCTTTCCGTG TGGGAGAGAT GGTCACTGTG GGCAAGGGTT TGGATGGAAA GCCAGACAAA 
CGCTGGTGCT TCAGGGTGGA CGAGGTGAAG TGGTCCCACT GGAATCAAAA TCTGGGCATC 
ATCAATGAGG ATCCAGGCCA GAAAGACCTT TCTGAACACA CACAGGGTGG ACGGGGCCTT 
CGAAGAGACC GCTGGTCCAC TGTAGTACCG AGAGTGGTTG AACTGAATCG CGGCTCCAGA 
GATCACACCG TAGAGATGGA GCCTCTCACA GGGAGACACC GACTCAAGTC TGAAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165208.1
CDS	373..2952
Translation	MTDQGFSASTLLKRYRLAMTESLSVSSPPDNSAQDSSEAADGDPNFPMSSMAALLENDDVSQPTHELPRPGQQNDQKQNMRIRFPGPFKKGVPNPMDLLESDYTEYPKQAPMDSMFDYGTCRQINNNKKGRRKKLPRGKAEIGMSCDEGSPEPPVLKVFNRWMLFEAVSRADPRALDGLLQYLQSHEKRLTDEEFKELSTGKTCLPKALLNLHNGQNDTIPILVDIAEQTGNLREFINTPFRDVYYRGQMALHIAIERRCKQYVELLVEKGADVHAQARGRFFQPRDEGGYFYFGELPLSLAACTNQPDMVHYLTENGHKKADLRRQDSRGNTVLHALVHIADNTRDNTRFVTKMFDLLLIKCAKLYPDCNLENILNNDGMSPLMMAAKLGKIGVFQHIIRREIKDEEARHLSRKFKDWAYGPVYSNLYDLSSLDTCGEEVSVLEILVYNSKIENRHEMLAVEPINELLRAKWQKFAAVTFYISVFSYLVTMIIFTLVAYYRPSVGKPPYAYDTTEDKVRLAGEIITVGSGLFFFVTNIKDLFLKKCPGVNSIFVDGSFQLLYFIYSVLVLVSAALYLSGIEAYVSVMVFALTLGWMNTLYFTRGLKLTGTYSIMIQKILIKDLFRFLLVYVLFMIGYASALVSLLTICPDKDTCKENCPTYPECRDTNTFSEFLLDLFKLTIGIGDLDNMLKGAQYPAVFLILLVTYIILTFVLLLNMLIALMGETVGQVSKESKKIWKLQWATTILDIERSFPVCLRRSFRVGEMVTVGKGLDGKPDKRWCFRVDEVKWSHWNQNLGIINEDPGQKDLSEHTQGGRGLRRDRWSTVVPRVVELNRGSRDHTVEMEPLTGRHRLKSES

Target ORF information:

RefSeq Version	XM_005165151.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165151.3

ORF Insert Sequence:

ATGACTGACC AGGGTTTTTC TGCTTCCACT CTACTGAAGC GCTATCGTCT GGCCATGACA 
GAGTCCTTGT CTGTCTCCTC ACCACCAGAC AATTCAGCTC AGGACTCTTC AGAGGCAGCA 
GATGGAGATC CCAACTTCCC CATGTCTTCA ATGGCAGCGC TACTGGAAAA TGATGATGTA 
TCACAGCCAA CTCATGAATT GCCTCGACCA GGACAACAGA ACGACCAGAA GCAGAACATG 
CGGATTAGGT TCCCAGGACC CTTTAAAAAG GGGGTTCCTA ACCCTATGGA TCTTCTTGAG 
TCGGATTATA CAGAGTATCC TAAACAAGCA CCTATGGATT CCATGTTTGA CTACGGCACA 
TGCAGGCAGA TCAACAACAA TAAGAAAGGA AGACGGAAGA AGCTTCCACG AGGGAAGGCA 
GAGATCGGGA TGTCATGTGA TGAGGGATCC CCGGAGCCCC CTGTTCTCAA AGTCTTCAAC 
CGCTGGATGT TGTTCGAAGC TGTATCTCGA GCAGATCCAA GAGCTCTGGA CGGCCTTCTG 
CAGTATCTTC AGTCCCACGA AAAGAGGCTG ACCGATGAAG AGTTCAAAGA ACTTTCTACA 
GGGAAAACCT GCCTACCAAA AGCACTTCTC AACCTGCACA ATGGTCAAAA CGACACCATC 
CCAATATTGG TGGACATTGC TGAACAGACA GGAAACCTCA GAGAGTTCAT CAACACACCC 
TTTAGAGATG TCTATTATAG GGGTCAGATG GCTCTGCATA TTGCTATAGA GCGACGATGC 
AAACAGTATG TGGAGCTTCT GGTGGAGAAG GGGGCTGATG TCCATGCGCA GGCCAGGGGA 
CGCTTCTTCC AGCCCAGAGA TGAGGGTGGA TACTTCTACT TTGGTGAACT GCCCCTCTCA 
CTCGCAGCTT GTACCAACCA ACCAGACATG GTGCATTACC TGACAGAGAA TGGACACAAG 
AAGGCGGATC TGAGGAGGCA GGACTCAAGG GGAAACACAG TCCTGCATGC TTTGGTCCAC 
ATTGCTGACA ACACTCGGGA CAACACACGT TTTGTCACTA AGATGTTTGA CTTGCTGCTC 
ATTAAATGTG CCAAACTCTA CCCGGATTGC AACCTGGAGA ATATACTCAA CAACGACGGC 
ATGTCCCCTC TCATGATGGC CGCCAAACTG GGCAAAATTG GGGTGTTTCA GCACATCATT 
CGGAGAGAGA TAAAAGATGA GGAAGCTCGA CACCTCTCAC GGAAATTTAA AGACTGGGCT 
TATGGACCAG TGTACTCCAA TCTGTATGAT CTGTCCTCCC TGGACACCTG CGGAGAGGAA 
GTGTCTGTTC TGGAAATACT CGTCTACAAC AGCAAGATTG AGAATCGGCA TGAGATGTTG 
GCGGTGGAAC CAATAAATGA ACTGCTGAGG GCCAAGTGGC AGAAATTTGC AGCGGTGACC 
TTCTACATCA GTGTGTTTTC CTATCTTGTG ACTATGATTA TCTTCACTCT TGTGGCCTAC 
TATCGCCCAT CAGTGGGAAA ACCCCCTTAT GCTTATGATA CCACAGAAGA CAAGGTGCGT 
CTGGCTGGAG AGATCATCAC AGTGGGTTCT GGACTCTTTT TCTTTGTAAC AAATATTAAG 
GACCTTTTCC TGAAGAAGTG CCCCGGGGTG AATTCTATAT TTGTTGATGG ATCCTTTCAG 
CTTCTCTACT TCATCTACTC TGTGTTGGTG CTGGTGAGCG CAGCGCTGTA TCTGTCAGGC 
ATTGAGGCCT ACGTGTCTGT GATGGTGTTT GCTTTAACAC TAGGCTGGAT GAACACCCTC 
TATTTTACCA GAGGCCTTAA ACTCACTGGA ACTTACAGCA TCATGATCCA AAAGATTCTC 
ATCAAAGATT TGTTCCGGTT CCTGCTGGTC TACGTGCTCT TCATGATTGG TTATGCTTCA 
GCATTAGTGT CGCTGTTGAC CATCTGCCCT GATAAGGACA CGTGTAAGGA AAACTGTCCT 
ACATATCCCG AATGTCGAGA CACAAACACC TTCAGTGAAT TCTTGCTGGA CCTCTTCAAG 
CTGACCATAG GCATCGGAGA CCTGGACAAC ATGCTGAAAG GCGCACAGTA TCCCGCCGTC 
TTCCTCATTC TGCTGGTCAC CTACATTATC CTCACCTTCG TCCTGTTGCT CAACATGTTA 
ATCGCTCTGA TGGGGGAGAC GGTGGGACAG GTGTCCAAAG AAAGCAAGAA GATCTGGAAA 
TTGCAGTGGG CGACTACTAT CCTGGACATT GAGCGTTCTT TCCCTGTGTG TCTGCGGCGG 
TCTTTCCGTG TGGGAGAGAT GGTCACTGTG GGCAAGGGTT TGGATGGAAA GCCAGACAAA 
CGCTGGTGCT TCAGGGTGGA CGAGGTGAAG TGGTCCCACT GGAATCAAAA TCTGGGCATC 
ATCAATGAGG ATCCAGGCCA GAAAGACCTT TCTGAACACA CACAGGGTGG ACGGGGCCTT 
CGAAGAGACC GCTGGTCCAC TGTAGTACCG AGAGTGGTTG AACTGAATCG CGGCTCCAGA 
GATCACACCG TAGAGATGGA GCCTCTCACA GGGAGACACC GACTCAAGTC TGAAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpv4 ( XM_005165152.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165209.1 Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10275
Clone ID Related Accession (Same CDS sequence)	XM_005165152.3 , XM_005165152.4
Accession Version	XM_005165152.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily V member 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165152.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGACG GTTTTTCTGC TTCCACTCTA CTGAAGCGCT ATCGTCTGGC CATGACAGAG 
TCCTTGTCTG TCTCCTCACC ACCAGACAAT TCAGCTCAGG ACTCTTCAGA GGCAGCAGAT 
GGAGATCCCA ACTTCCCCAT GTCTTCAATG GCAGCGCTAC TGGAAAATGA TGATGTATCA 
CAGCCAACTC ATGAATTGCC TCGACCAGGA CAACAGAACG ACCAGAAGCA GAACATGCGG 
ATTAGGTTCC CAGGACCCTT TAAAAAGGGG GTTCCTAACC CTATGGATCT TCTTGAGTCG 
GATTATACAG AGTATCCTAA ACAAGCACCT ATGGATTCCA TGTTTGACTA CGGCACATGC 
AGGCAGATCA ACAACAATAA GAAAGGAAGA CGGAAGAAGC TTCCACGAGG GAAGGCAGAG 
ATCGGGATGT CATGTGATGA GGGATCCCCG GAGCCCCCTG TTCTCAAAGT CTTCAACCGC 
TGGATGTTGT TCGAAGCTGT ATCTCGAGCA GATCCAAGAG CTCTGGACGG CCTTCTGCAG 
TATCTTCAGT CCCACGAAAA GAGGCTGACC GATGAAGAGT TCAAAGAACT TTCTACAGGG 
AAAACCTGCC TACCAAAAGC ACTTCTCAAC CTGCACAATG GTCAAAACGA CACCATCCCA 
ATATTGGTGG ACATTGCTGA ACAGACAGGA AACCTCAGAG AGTTCATCAA CACACCCTTT 
AGAGATGTCT ATTATAGGGG TCAGATGGCT CTGCATATTG CTATAGAGCG ACGATGCAAA 
CAGTATGTGG AGCTTCTGGT GGAGAAGGGG GCTGATGTCC ATGCGCAGGC CAGGGGACGC 
TTCTTCCAGC CCAGAGATGA GGGTGGATAC TTCTACTTTG GTGAACTGCC CCTCTCACTC 
GCAGCTTGTA CCAACCAACC AGACATGGTG CATTACCTGA CAGAGAATGG ACACAAGAAG 
GCGGATCTGA GGAGGCAGGA CTCAAGGGGA AACACAGTCC TGCATGCTTT GGTCCACATT 
GCTGACAACA CTCGGGACAA CACACGTTTT GTCACTAAGA TGTTTGACTT GCTGCTCATT 
AAATGTGCCA AACTCTACCC GGATTGCAAC CTGGAGAATA TACTCAACAA CGACGGCATG 
TCCCCTCTCA TGATGGCCGC CAAACTGGGC AAAATTGGGG TGTTTCAGCA CATCATTCGG 
AGAGAGATAA AAGATGAGGA AGCTCGACAC CTCTCACGGA AATTTAAAGA CTGGGCTTAT 
GGACCAGTGT ACTCCAATCT GTATGATCTG TCCTCCCTGG ACACCTGCGG AGAGGAAGTG 
TCTGTTCTGG AAATACTCGT CTACAACAGC AAGATTGAGA ATCGGCATGA GATGTTGGCG 
GTGGAACCAA TAAATGAACT GCTGAGGGCC AAGTGGCAGA AATTTGCAGC GGTGACCTTC 
TACATCAGTG TGTTTTCCTA TCTTGTGACT ATGATTATCT TCACTCTTGT GGCCTACTAT 
CGCCCATCAG TGGGAAAACC CCCTTATGCT TATGATACCA CAGAAGACAA GGTGCGTCTG 
GCTGGAGAGA TCATCACAGT GGGTTCTGGA CTCTTTTTCT TTGTAACAAA TATTAAGGAC 
CTTTTCCTGA AGAAGTGCCC CGGGGTGAAT TCTATATTTG TTGATGGATC CTTTCAGCTT 
CTCTACTTCA TCTACTCTGT GTTGGTGCTG GTGAGCGCAG CGCTGTATCT GTCAGGCATT 
GAGGCCTACG TGTCTGTGAT GGTGTTTGCT TTAACACTAG GCTGGATGAA CACCCTCTAT 
TTTACCAGAG GCCTTAAACT CACTGGAACT TACAGCATCA TGATCCAAAA GATTCTCATC 
AAAGATTTGT TCCGGTTCCT GCTGGTCTAC GTGCTCTTCA TGATTGGTTA TGCTTCAGCA 
TTAGTGTCGC TGTTGACCAT CTGCCCTGAT AAGGACACGT GTAAGGAAAA CTGTCCTACA 
TATCCCGAAT GTCGAGACAC AAACACCTTC AGTGAATTCT TGCTGGACCT CTTCAAGCTG 
ACCATAGGCA TCGGAGACCT GGACAACATG CTGAAAGGCG CACAGTATCC CGCCGTCTTC 
CTCATTCTGC TGGTCACCTA CATTATCCTC ACCTTCGTCC TGTTGCTCAA CATGTTAATC 
GCTCTGATGG GGGAGACGGT GGGACAGGTG TCCAAAGAAA GCAAGAAGAT CTGGAAATTG 
CAGTGGGCGA CTACTATCCT GGACATTGAG CGTTCTTTCC CTGTGTGTCT GCGGCGGTCT 
TTCCGTGTGG GAGAGATGGT CACTGTGGGC AAGGGTTTGG ATGGAAAGCC AGACAAACGC 
TGGTGCTTCA GGGTGGACGA GGTGAAGTGG TCCCACTGGA ATCAAAATCT GGGCATCATC 
AATGAGGATC CAGGCCAGAA AGACCTTTCT GAACACACAC AGGGTGGACG GGGCCTTCGA 
AGAGACCGCT GGTCCACTGT AGTACCGAGA GTGGTTGAAC TGAATCGCGG CTCCAGAGAT 
CACACCGTAG AGATGGAGCC TCTCACAGGG AGACACCGAC TCAAGTCTGA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165209.1
CDS	373..2949
Translation	MTDGFSASTLLKRYRLAMTESLSVSSPPDNSAQDSSEAADGDPNFPMSSMAALLENDDVSQPTHELPRPGQQNDQKQNMRIRFPGPFKKGVPNPMDLLESDYTEYPKQAPMDSMFDYGTCRQINNNKKGRRKKLPRGKAEIGMSCDEGSPEPPVLKVFNRWMLFEAVSRADPRALDGLLQYLQSHEKRLTDEEFKELSTGKTCLPKALLNLHNGQNDTIPILVDIAEQTGNLREFINTPFRDVYYRGQMALHIAIERRCKQYVELLVEKGADVHAQARGRFFQPRDEGGYFYFGELPLSLAACTNQPDMVHYLTENGHKKADLRRQDSRGNTVLHALVHIADNTRDNTRFVTKMFDLLLIKCAKLYPDCNLENILNNDGMSPLMMAAKLGKIGVFQHIIRREIKDEEARHLSRKFKDWAYGPVYSNLYDLSSLDTCGEEVSVLEILVYNSKIENRHEMLAVEPINELLRAKWQKFAAVTFYISVFSYLVTMIIFTLVAYYRPSVGKPPYAYDTTEDKVRLAGEIITVGSGLFFFVTNIKDLFLKKCPGVNSIFVDGSFQLLYFIYSVLVLVSAALYLSGIEAYVSVMVFALTLGWMNTLYFTRGLKLTGTYSIMIQKILIKDLFRFLLVYVLFMIGYASALVSLLTICPDKDTCKENCPTYPECRDTNTFSEFLLDLFKLTIGIGDLDNMLKGAQYPAVFLILLVTYIILTFVLLLNMLIALMGETVGQVSKESKKIWKLQWATTILDIERSFPVCLRRSFRVGEMVTVGKGLDGKPDKRWCFRVDEVKWSHWNQNLGIINEDPGQKDLSEHTQGGRGLRRDRWSTVVPRVVELNRGSRDHTVEMEPLTGRHRLKSES

Target ORF information:

RefSeq Version	XM_005165152.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165152.3

ORF Insert Sequence:

ATGACTGACG GTTTTTCTGC TTCCACTCTA CTGAAGCGCT ATCGTCTGGC CATGACAGAG 
TCCTTGTCTG TCTCCTCACC ACCAGACAAT TCAGCTCAGG ACTCTTCAGA GGCAGCAGAT 
GGAGATCCCA ACTTCCCCAT GTCTTCAATG GCAGCGCTAC TGGAAAATGA TGATGTATCA 
CAGCCAACTC ATGAATTGCC TCGACCAGGA CAACAGAACG ACCAGAAGCA GAACATGCGG 
ATTAGGTTCC CAGGACCCTT TAAAAAGGGG GTTCCTAACC CTATGGATCT TCTTGAGTCG 
GATTATACAG AGTATCCTAA ACAAGCACCT ATGGATTCCA TGTTTGACTA CGGCACATGC 
AGGCAGATCA ACAACAATAA GAAAGGAAGA CGGAAGAAGC TTCCACGAGG GAAGGCAGAG 
ATCGGGATGT CATGTGATGA GGGATCCCCG GAGCCCCCTG TTCTCAAAGT CTTCAACCGC 
TGGATGTTGT TCGAAGCTGT ATCTCGAGCA GATCCAAGAG CTCTGGACGG CCTTCTGCAG 
TATCTTCAGT CCCACGAAAA GAGGCTGACC GATGAAGAGT TCAAAGAACT TTCTACAGGG 
AAAACCTGCC TACCAAAAGC ACTTCTCAAC CTGCACAATG GTCAAAACGA CACCATCCCA 
ATATTGGTGG ACATTGCTGA ACAGACAGGA AACCTCAGAG AGTTCATCAA CACACCCTTT 
AGAGATGTCT ATTATAGGGG TCAGATGGCT CTGCATATTG CTATAGAGCG ACGATGCAAA 
CAGTATGTGG AGCTTCTGGT GGAGAAGGGG GCTGATGTCC ATGCGCAGGC CAGGGGACGC 
TTCTTCCAGC CCAGAGATGA GGGTGGATAC TTCTACTTTG GTGAACTGCC CCTCTCACTC 
GCAGCTTGTA CCAACCAACC AGACATGGTG CATTACCTGA CAGAGAATGG ACACAAGAAG 
GCGGATCTGA GGAGGCAGGA CTCAAGGGGA AACACAGTCC TGCATGCTTT GGTCCACATT 
GCTGACAACA CTCGGGACAA CACACGTTTT GTCACTAAGA TGTTTGACTT GCTGCTCATT 
AAATGTGCCA AACTCTACCC GGATTGCAAC CTGGAGAATA TACTCAACAA CGACGGCATG 
TCCCCTCTCA TGATGGCCGC CAAACTGGGC AAAATTGGGG TGTTTCAGCA CATCATTCGG 
AGAGAGATAA AAGATGAGGA AGCTCGACAC CTCTCACGGA AATTTAAAGA CTGGGCTTAT 
GGACCAGTGT ACTCCAATCT GTATGATCTG TCCTCCCTGG ACACCTGCGG AGAGGAAGTG 
TCTGTTCTGG AAATACTCGT CTACAACAGC AAGATTGAGA ATCGGCATGA GATGTTGGCG 
GTGGAACCAA TAAATGAACT GCTGAGGGCC AAGTGGCAGA AATTTGCAGC GGTGACCTTC 
TACATCAGTG TGTTTTCCTA TCTTGTGACT ATGATTATCT TCACTCTTGT GGCCTACTAT 
CGCCCATCAG TGGGAAAACC CCCTTATGCT TATGATACCA CAGAAGACAA GGTGCGTCTG 
GCTGGAGAGA TCATCACAGT GGGTTCTGGA CTCTTTTTCT TTGTAACAAA TATTAAGGAC 
CTTTTCCTGA AGAAGTGCCC CGGGGTGAAT TCTATATTTG TTGATGGATC CTTTCAGCTT 
CTCTACTTCA TCTACTCTGT GTTGGTGCTG GTGAGCGCAG CGCTGTATCT GTCAGGCATT 
GAGGCCTACG TGTCTGTGAT GGTGTTTGCT TTAACACTAG GCTGGATGAA CACCCTCTAT 
TTTACCAGAG GCCTTAAACT CACTGGAACT TACAGCATCA TGATCCAAAA GATTCTCATC 
AAAGATTTGT TCCGGTTCCT GCTGGTCTAC GTGCTCTTCA TGATTGGTTA TGCTTCAGCA 
TTAGTGTCGC TGTTGACCAT CTGCCCTGAT AAGGACACGT GTAAGGAAAA CTGTCCTACA 
TATCCCGAAT GTCGAGACAC AAACACCTTC AGTGAATTCT TGCTGGACCT CTTCAAGCTG 
ACCATAGGCA TCGGAGACCT GGACAACATG CTGAAAGGCG CACAGTATCC CGCCGTCTTC 
CTCATTCTGC TGGTCACCTA CATTATCCTC ACCTTCGTCC TGTTGCTCAA CATGTTAATC 
GCTCTGATGG GGGAGACGGT GGGACAGGTG TCCAAAGAAA GCAAGAAGAT CTGGAAATTG 
CAGTGGGCGA CTACTATCCT GGACATTGAG CGTTCTTTCC CTGTGTGTCT GCGGCGGTCT 
TTCCGTGTGG GAGAGATGGT CACTGTGGGC AAGGGTTTGG ATGGAAAGCC AGACAAACGC 
TGGTGCTTCA GGGTGGACGA GGTGAAGTGG TCCCACTGGA ATCAAAATCT GGGCATCATC 
AATGAGGATC CAGGCCAGAA AGACCTTTCT GAACACACAC AGGGTGGACG GGGCCTTCGA 
AGAGACCGCT GGTCCACTGT AGTACCGAGA GTGGTTGAAC TGAATCGCGG CTCCAGAGAT 
CACACCGTAG AGATGGAGCC TCTCACAGGG AGACACCGAC TCAAGTCTGA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpv4 ( XM_005165152.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165209.1 Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10275
Clone ID Related Accession (Same CDS sequence)	XM_005165152.3 , XM_005165152.4
Accession Version	XM_005165152.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily V member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165152.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGACG GTTTTTCTGC TTCCACTCTA CTGAAGCGCT ATCGTCTGGC CATGACAGAG 
TCCTTGTCTG TCTCCTCACC ACCAGACAAT TCAGCTCAGG ACTCTTCAGA GGCAGCAGAT 
GGAGATCCCA ACTTCCCCAT GTCTTCAATG GCAGCGCTAC TGGAAAATGA TGATGTATCA 
CAGCCAACTC ATGAATTGCC TCGACCAGGA CAACAGAACG ACCAGAAGCA GAACATGCGG 
ATTAGGTTCC CAGGACCCTT TAAAAAGGGG GTTCCTAACC CTATGGATCT TCTTGAGTCG 
GATTATACAG AGTATCCTAA ACAAGCACCT ATGGATTCCA TGTTTGACTA CGGCACATGC 
AGGCAGATCA ACAACAATAA GAAAGGAAGA CGGAAGAAGC TTCCACGAGG GAAGGCAGAG 
ATCGGGATGT CATGTGATGA GGGATCCCCG GAGCCCCCTG TTCTCAAAGT CTTCAACCGC 
TGGATGTTGT TCGAAGCTGT ATCTCGAGCA GATCCAAGAG CTCTGGACGG CCTTCTGCAG 
TATCTTCAGT CCCACGAAAA GAGGCTGACC GATGAAGAGT TCAAAGAACT TTCTACAGGG 
AAAACCTGCC TACCAAAAGC ACTTCTCAAC CTGCACAATG GTCAAAACGA CACCATCCCA 
ATATTGGTGG ACATTGCTGA ACAGACAGGA AACCTCAGAG AGTTCATCAA CACACCCTTT 
AGAGATGTCT ATTATAGGGG TCAGATGGCT CTGCATATTG CTATAGAGCG ACGATGCAAA 
CAGTATGTGG AGCTTCTGGT GGAGAAGGGG GCTGATGTCC ATGCGCAGGC CAGGGGACGC 
TTCTTCCAGC CCAGAGATGA GGGTGGATAC TTCTACTTTG GTGAACTGCC CCTCTCACTC 
GCAGCTTGTA CCAACCAACC AGACATGGTG CATTACCTGA CAGAGAATGG ACACAAGAAG 
GCGGATCTGA GGAGGCAGGA CTCAAGGGGA AACACAGTCC TGCATGCTTT GGTCCACATT 
GCTGACAACA CTCGGGACAA CACACGTTTT GTCACTAAGA TGTTTGACTT GCTGCTCATT 
AAATGTGCCA AACTCTACCC GGATTGCAAC CTGGAGAATA TACTCAACAA CGACGGCATG 
TCCCCTCTCA TGATGGCCGC CAAACTGGGC AAAATTGGGG TGTTTCAGCA CATCATTCGG 
AGAGAGATAA AAGATGAGGA AGCTCGACAC CTCTCACGGA AATTTAAAGA CTGGGCTTAT 
GGACCAGTGT ACTCCAATCT GTATGATCTG TCCTCCCTGG ACACCTGCGG AGAGGAAGTG 
TCTGTTCTGG AAATACTCGT CTACAACAGC AAGATTGAGA ATCGGCATGA GATGTTGGCG 
GTGGAACCAA TAAATGAACT GCTGAGGGCC AAGTGGCAGA AATTTGCAGC GGTGACCTTC 
TACATCAGTG TGTTTTCCTA TCTTGTGACT ATGATTATCT TCACTCTTGT GGCCTACTAT 
CGCCCATCAG TGGGAAAACC CCCTTATGCT TATGATACCA CAGAAGACAA GGTGCGTCTG 
GCTGGAGAGA TCATCACAGT GGGTTCTGGA CTCTTTTTCT TTGTAACAAA TATTAAGGAC 
CTTTTCCTGA AGAAGTGCCC CGGGGTGAAT TCTATATTTG TTGATGGATC CTTTCAGCTT 
CTCTACTTCA TCTACTCTGT GTTGGTGCTG GTGAGCGCAG CGCTGTATCT GTCAGGCATT 
GAGGCCTACG TGTCTGTGAT GGTGTTTGCT TTAACACTAG GCTGGATGAA CACCCTCTAT 
TTTACCAGAG GCCTTAAACT CACTGGAACT TACAGCATCA TGATCCAAAA GATTCTCATC 
AAAGATTTGT TCCGGTTCCT GCTGGTCTAC GTGCTCTTCA TGATTGGTTA TGCTTCAGCA 
TTAGTGTCGC TGTTGACCAT CTGCCCTGAT AAGGACACGT GTAAGGAAAA CTGTCCTACA 
TATCCCGAAT GTCGAGACAC AAACACCTTC AGTGAATTCT TGCTGGACCT CTTCAAGCTG 
ACCATAGGCA TCGGAGACCT GGACAACATG CTGAAAGGCG CACAGTATCC CGCCGTCTTC 
CTCATTCTGC TGGTCACCTA CATTATCCTC ACCTTCGTCC TGTTGCTCAA CATGTTAATC 
GCTCTGATGG GGGAGACGGT GGGACAGGTG TCCAAAGAAA GCAAGAAGAT CTGGAAATTG 
CAGTGGGCGA CTACTATCCT GGACATTGAG CGTTCTTTCC CTGTGTGTCT GCGGCGGTCT 
TTCCGTGTGG GAGAGATGGT CACTGTGGGC AAGGGTTTGG ATGGAAAGCC AGACAAACGC 
TGGTGCTTCA GGGTGGACGA GGTGAAGTGG TCCCACTGGA ATCAAAATCT GGGCATCATC 
AATGAGGATC CAGGCCAGAA AGACCTTTCT GAACACACAC AGGGTGGACG GGGCCTTCGA 
AGAGACCGCT GGTCCACTGT AGTACCGAGA GTGGTTGAAC TGAATCGCGG CTCCAGAGAT 
CACACCGTAG AGATGGAGCC TCTCACAGGG AGACACCGAC TCAAGTCTGA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165209.1
CDS	400..2976
Translation	MTDGFSASTLLKRYRLAMTESLSVSSPPDNSAQDSSEAADGDPNFPMSSMAALLENDDVSQPTHELPRPGQQNDQKQNMRIRFPGPFKKGVPNPMDLLESDYTEYPKQAPMDSMFDYGTCRQINNNKKGRRKKLPRGKAEIGMSCDEGSPEPPVLKVFNRWMLFEAVSRADPRALDGLLQYLQSHEKRLTDEEFKELSTGKTCLPKALLNLHNGQNDTIPILVDIAEQTGNLREFINTPFRDVYYRGQMALHIAIERRCKQYVELLVEKGADVHAQARGRFFQPRDEGGYFYFGELPLSLAACTNQPDMVHYLTENGHKKADLRRQDSRGNTVLHALVHIADNTRDNTRFVTKMFDLLLIKCAKLYPDCNLENILNNDGMSPLMMAAKLGKIGVFQHIIRREIKDEEARHLSRKFKDWAYGPVYSNLYDLSSLDTCGEEVSVLEILVYNSKIENRHEMLAVEPINELLRAKWQKFAAVTFYISVFSYLVTMIIFTLVAYYRPSVGKPPYAYDTTEDKVRLAGEIITVGSGLFFFVTNIKDLFLKKCPGVNSIFVDGSFQLLYFIYSVLVLVSAALYLSGIEAYVSVMVFALTLGWMNTLYFTRGLKLTGTYSIMIQKILIKDLFRFLLVYVLFMIGYASALVSLLTICPDKDTCKENCPTYPECRDTNTFSEFLLDLFKLTIGIGDLDNMLKGAQYPAVFLILLVTYIILTFVLLLNMLIALMGETVGQVSKESKKIWKLQWATTILDIERSFPVCLRRSFRVGEMVTVGKGLDGKPDKRWCFRVDEVKWSHWNQNLGIINEDPGQKDLSEHTQGGRGLRRDRWSTVVPRVVELNRGSRDHTVEMEPLTGRHRLKSES

Target ORF information:

RefSeq Version	XM_005165152.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165152.4

ORF Insert Sequence:

ATGACTGACG GTTTTTCTGC TTCCACTCTA CTGAAGCGCT ATCGTCTGGC CATGACAGAG 
TCCTTGTCTG TCTCCTCACC ACCAGACAAT TCAGCTCAGG ACTCTTCAGA GGCAGCAGAT 
GGAGATCCCA ACTTCCCCAT GTCTTCAATG GCAGCGCTAC TGGAAAATGA TGATGTATCA 
CAGCCAACTC ATGAATTGCC TCGACCAGGA CAACAGAACG ACCAGAAGCA GAACATGCGG 
ATTAGGTTCC CAGGACCCTT TAAAAAGGGG GTTCCTAACC CTATGGATCT TCTTGAGTCG 
GATTATACAG AGTATCCTAA ACAAGCACCT ATGGATTCCA TGTTTGACTA CGGCACATGC 
AGGCAGATCA ACAACAATAA GAAAGGAAGA CGGAAGAAGC TTCCACGAGG GAAGGCAGAG 
ATCGGGATGT CATGTGATGA GGGATCCCCG GAGCCCCCTG TTCTCAAAGT CTTCAACCGC 
TGGATGTTGT TCGAAGCTGT ATCTCGAGCA GATCCAAGAG CTCTGGACGG CCTTCTGCAG 
TATCTTCAGT CCCACGAAAA GAGGCTGACC GATGAAGAGT TCAAAGAACT TTCTACAGGG 
AAAACCTGCC TACCAAAAGC ACTTCTCAAC CTGCACAATG GTCAAAACGA CACCATCCCA 
ATATTGGTGG ACATTGCTGA ACAGACAGGA AACCTCAGAG AGTTCATCAA CACACCCTTT 
AGAGATGTCT ATTATAGGGG TCAGATGGCT CTGCATATTG CTATAGAGCG ACGATGCAAA 
CAGTATGTGG AGCTTCTGGT GGAGAAGGGG GCTGATGTCC ATGCGCAGGC CAGGGGACGC 
TTCTTCCAGC CCAGAGATGA GGGTGGATAC TTCTACTTTG GTGAACTGCC CCTCTCACTC 
GCAGCTTGTA CCAACCAACC AGACATGGTG CATTACCTGA CAGAGAATGG ACACAAGAAG 
GCGGATCTGA GGAGGCAGGA CTCAAGGGGA AACACAGTCC TGCATGCTTT GGTCCACATT 
GCTGACAACA CTCGGGACAA CACACGTTTT GTCACTAAGA TGTTTGACTT GCTGCTCATT 
AAATGTGCCA AACTCTACCC GGATTGCAAC CTGGAGAATA TACTCAACAA CGACGGCATG 
TCCCCTCTCA TGATGGCCGC CAAACTGGGC AAAATTGGGG TGTTTCAGCA CATCATTCGG 
AGAGAGATAA AAGATGAGGA AGCTCGACAC CTCTCACGGA AATTTAAAGA CTGGGCTTAT 
GGACCAGTGT ACTCCAATCT GTATGATCTG TCCTCCCTGG ACACCTGCGG AGAGGAAGTG 
TCTGTTCTGG AAATACTCGT CTACAACAGC AAGATTGAGA ATCGGCATGA GATGTTGGCG 
GTGGAACCAA TAAATGAACT GCTGAGGGCC AAGTGGCAGA AATTTGCAGC GGTGACCTTC 
TACATCAGTG TGTTTTCCTA TCTTGTGACT ATGATTATCT TCACTCTTGT GGCCTACTAT 
CGCCCATCAG TGGGAAAACC CCCTTATGCT TATGATACCA CAGAAGACAA GGTGCGTCTG 
GCTGGAGAGA TCATCACAGT GGGTTCTGGA CTCTTTTTCT TTGTAACAAA TATTAAGGAC 
CTTTTCCTGA AGAAGTGCCC CGGGGTGAAT TCTATATTTG TTGATGGATC CTTTCAGCTT 
CTCTACTTCA TCTACTCTGT GTTGGTGCTG GTGAGCGCAG CGCTGTATCT GTCAGGCATT 
GAGGCCTACG TGTCTGTGAT GGTGTTTGCT TTAACACTAG GCTGGATGAA CACCCTCTAT 
TTTACCAGAG GCCTTAAACT CACTGGAACT TACAGCATCA TGATCCAAAA GATTCTCATC 
AAAGATTTGT TCCGGTTCCT GCTGGTCTAC GTGCTCTTCA TGATTGGTTA TGCTTCAGCA 
TTAGTGTCGC TGTTGACCAT CTGCCCTGAT AAGGACACGT GTAAGGAAAA CTGTCCTACA 
TATCCCGAAT GTCGAGACAC AAACACCTTC AGTGAATTCT TGCTGGACCT CTTCAAGCTG 
ACCATAGGCA TCGGAGACCT GGACAACATG CTGAAAGGCG CACAGTATCC CGCCGTCTTC 
CTCATTCTGC TGGTCACCTA CATTATCCTC ACCTTCGTCC TGTTGCTCAA CATGTTAATC 
GCTCTGATGG GGGAGACGGT GGGACAGGTG TCCAAAGAAA GCAAGAAGAT CTGGAAATTG 
CAGTGGGCGA CTACTATCCT GGACATTGAG CGTTCTTTCC CTGTGTGTCT GCGGCGGTCT 
TTCCGTGTGG GAGAGATGGT CACTGTGGGC AAGGGTTTGG ATGGAAAGCC AGACAAACGC 
TGGTGCTTCA GGGTGGACGA GGTGAAGTGG TCCCACTGGA ATCAAAATCT GGGCATCATC 
AATGAGGATC CAGGCCAGAA AGACCTTTCT GAACACACAC AGGGTGGACG GGGCCTTCGA 
AGAGACCGCT GGTCCACTGT AGTACCGAGA GTGGTTGAAC TGAATCGCGG CTCCAGAGAT 
CACACCGTAG AGATGGAGCC TCTCACAGGG AGACACCGAC TCAAGTCTGA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpv4 ( XM_009301306.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299581.1 Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa42686
Clone ID Related Accession (Same CDS sequence)	XM_009301306.2
Accession Version	XM_009301306.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily V member 4 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301306.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAGT CCTTGTCTGT CTCCTCACCA CCAGACAATT CAGCTCAGGA CTCTTCAGAG 
GCAGCAGATG GAGATCCCAA CTTCCCCATG TCTTCAATGG CAGCGCTACT GGAAAATGAT 
GATGTATCAC AGCCAACTCA TGAATTGCCT CGACCAGGAC AACAGAACGA CCAGAAGCAG 
AACATGCGGA TTAGGTTCCC AGGACCCTTT AAAAAGGGGG TTCCTAACCC TATGGATCTT 
CTTGAGTCGG ATTATACAGA GTATCCTAAA CAAGCACCTA TGGATTCCAT GTTTGACTAC 
GGCACATGCA GGCAGATCAA CAACAATAAG AAAGGAAGAC GGAAGAAGCT TCCACGAGGG 
AAGGCAGAGA TCGGGATGTC ATGTGATGAG GGATCCCCGG AGCCCCCTGT TCTCAAAGTC 
TTCAACCGCT GGATGTTGTT CGAAGCTGTA TCTCGAGCAG ATCCAAGAGC TCTGGACGGC 
CTTCTGCAGT ATCTTCAGTC CCACGAAAAG AGGCTGACCG ATGAAGAGTT CAAAGAACTT 
TCTACAGGGA AAACCTGCCT ACCAAAAGCA CTTCTCAACC TGCACAATGG TCAAAACGAC 
ACCATCCCAA TATTGGTGGA CATTGCTGAA CAGACAGGAA ACCTCAGAGA GTTCATCAAC 
ACACCCTTTA GAGATGTCTA TTATAGGGGT CAGATGGCTC TGCATATTGC TATAGAGCGA 
CGATGCAAAC AGTATGTGGA GCTTCTGGTG GAGAAGGGGG CTGATGTCCA TGCGCAGGCC 
AGGGGACGCT TCTTCCAGCC CAGAGATGAG GGTGGATACT TCTACTTTGG TGAACTGCCC 
CTCTCACTCG CAGCTTGTAC CAACCAACCA GACATGGTGC ATTACCTGAC AGAGAATGGA 
CACAAGAAGG CGGATCTGAG GAGGCAGGAC TCAAGGGGAA ACACAGTCCT GCATGCTTTG 
GTCCACATTG CTGACAACAC TCGGGACAAC ACACGTTTTG TCACTAAGAT GTTTGACTTG 
CTGCTCATTA AATGTGCCAA ACTCTACCCG GATTGCAACC TGGAGAATAT ACTCAACAAC 
GACGGCATGT CCCCTCTCAT GATGGCCGCC AAACTGGGCA AAATTGGGGT GTTTCAGCAC 
ATCATTCGGA GAGAGATAAA AGATGAGGAA GCTCGACACC TCTCACGGAA ATTTAAAGAC 
TGGGCTTATG GACCAGTGTA CTCCAATCTG TATGATCTGT CCTCCCTGGA CACCTGCGGA 
GAGGAAGTGT CTGTTCTGGA AATACTCGTC TACAACAGCA AGATTGAGAA TCGGCATGAG 
ATGTTGGCGG TGGAACCAAT AAATGAACTG CTGAGGGCCA AGTGGCAGAA ATTTGCAGCG 
GTGACCTTCT ACATCAGTGT GTTTTCCTAT CTTGTGACTA TGATTATCTT CACTCTTGTG 
GCCTACTATC GCCCATCAGT GGGAAAACCC CCTTATGCTT ATGATACCAC AGAAGACAAG 
GTGCGTCTGG CTGGAGAGAT CATCACAGTG GGTTCTGGAC TCTTTTTCTT TGTAACAAAT 
ATTAAGGACC TTTTCCTGAA GAAGTGCCCC GGGGTGAATT CTATATTTGT TGATGGATCC 
TTTCAGCTTC TCTACTTCAT CTACTCTGTG TTGGTGCTGG TGAGCGCAGC GCTGTATCTG 
TCAGGCATTG AGGCCTACGT GTCTGTGATG GTGTTTGCTT TAACACTAGG CTGGATGAAC 
ACCCTCTATT TTACCAGAGG CCTTAAACTC ACTGGAACTT ACAGCATCAT GATCCAAAAG 
ATTCTCATCA AAGATTTGTT CCGGTTCCTG CTGGTCTACG TGCTCTTCAT GATTGGTTAT 
GCTTCAGCAT TAGTGTCGCT GTTGACCATC TGCCCTGATA AGGACACGTG TAAGGAAAAC 
TGTCCTACAT ATCCCGAATG TCGAGACACA AACACCTTCA GTGAATTCTT GCTGGACCTC 
TTCAAGCTGA CCATAGGCAT CGGAGACCTG GACAACATGC TGAAAGGCGC ACAGTATCCC 
GCCGTCTTCC TCATTCTGCT GGTCACCTAC ATTATCCTCA CCTTCGTCCT GTTGCTCAAC 
ATGTTAATCG CTCTGATGGG GGAGACGGTG GGACAGGTGT CCAAAGAAAG CAAGAAGATC 
TGGAAATTGC AGTGGGCGAC TACTATCCTG GACATTGAGC GTTCTTTCCC TGTGTGTCTG 
CGGCGGTCTT TCCGTGTGGG AGAGATGGTC ACTGTGGGCA AGGGTTTGGA TGGAAAGCCA 
GACAAACGCT GGTGCTTCAG GGTGGACGAG GTGAAGTGGT CCCACTGGAA TCAAAATCTG 
GGCATCATCA ATGAGGATCC AGGCCAGAAA GACCTTTCTG AACACACACA GGGTGGACGG 
GGCCTTCGAA GAGACCGCTG GTCCACTGTA GTACCGAGAG TGGTTGAACT GAATCGCGGC 
TCCAGAGATC ACACCGTAGA GATGGAGCCT CTCACAGGGA GACACCGACT CAAGTCTGAA 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299581.1
CDS	326..2851
Translation	MTESLSVSSPPDNSAQDSSEAADGDPNFPMSSMAALLENDDVSQPTHELPRPGQQNDQKQNMRIRFPGPFKKGVPNPMDLLESDYTEYPKQAPMDSMFDYGTCRQINNNKKGRRKKLPRGKAEIGMSCDEGSPEPPVLKVFNRWMLFEAVSRADPRALDGLLQYLQSHEKRLTDEEFKELSTGKTCLPKALLNLHNGQNDTIPILVDIAEQTGNLREFINTPFRDVYYRGQMALHIAIERRCKQYVELLVEKGADVHAQARGRFFQPRDEGGYFYFGELPLSLAACTNQPDMVHYLTENGHKKADLRRQDSRGNTVLHALVHIADNTRDNTRFVTKMFDLLLIKCAKLYPDCNLENILNNDGMSPLMMAAKLGKIGVFQHIIRREIKDEEARHLSRKFKDWAYGPVYSNLYDLSSLDTCGEEVSVLEILVYNSKIENRHEMLAVEPINELLRAKWQKFAAVTFYISVFSYLVTMIIFTLVAYYRPSVGKPPYAYDTTEDKVRLAGEIITVGSGLFFFVTNIKDLFLKKCPGVNSIFVDGSFQLLYFIYSVLVLVSAALYLSGIEAYVSVMVFALTLGWMNTLYFTRGLKLTGTYSIMIQKILIKDLFRFLLVYVLFMIGYASALVSLLTICPDKDTCKENCPTYPECRDTNTFSEFLLDLFKLTIGIGDLDNMLKGAQYPAVFLILLVTYIILTFVLLLNMLIALMGETVGQVSKESKKIWKLQWATTILDIERSFPVCLRRSFRVGEMVTVGKGLDGKPDKRWCFRVDEVKWSHWNQNLGIINEDPGQKDLSEHTQGGRGLRRDRWSTVVPRVVELNRGSRDHTVEMEPLTGRHRLKSES

Target ORF information:

RefSeq Version	XM_009301306.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily V, member 4 (trpv4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301306.2

ORF Insert Sequence:

ATGACAGAGT CCTTGTCTGT CTCCTCACCA CCAGACAATT CAGCTCAGGA CTCTTCAGAG 
GCAGCAGATG GAGATCCCAA CTTCCCCATG TCTTCAATGG CAGCGCTACT GGAAAATGAT 
GATGTATCAC AGCCAACTCA TGAATTGCCT CGACCAGGAC AACAGAACGA CCAGAAGCAG 
AACATGCGGA TTAGGTTCCC AGGACCCTTT AAAAAGGGGG TTCCTAACCC TATGGATCTT 
CTTGAGTCGG ATTATACAGA GTATCCTAAA CAAGCACCTA TGGATTCCAT GTTTGACTAC 
GGCACATGCA GGCAGATCAA CAACAATAAG AAAGGAAGAC GGAAGAAGCT TCCACGAGGG 
AAGGCAGAGA TCGGGATGTC ATGTGATGAG GGATCCCCGG AGCCCCCTGT TCTCAAAGTC 
TTCAACCGCT GGATGTTGTT CGAAGCTGTA TCTCGAGCAG ATCCAAGAGC TCTGGACGGC 
CTTCTGCAGT ATCTTCAGTC CCACGAAAAG AGGCTGACCG ATGAAGAGTT CAAAGAACTT 
TCTACAGGGA AAACCTGCCT ACCAAAAGCA CTTCTCAACC TGCACAATGG TCAAAACGAC 
ACCATCCCAA TATTGGTGGA CATTGCTGAA CAGACAGGAA ACCTCAGAGA GTTCATCAAC 
ACACCCTTTA GAGATGTCTA TTATAGGGGT CAGATGGCTC TGCATATTGC TATAGAGCGA 
CGATGCAAAC AGTATGTGGA GCTTCTGGTG GAGAAGGGGG CTGATGTCCA TGCGCAGGCC 
AGGGGACGCT TCTTCCAGCC CAGAGATGAG GGTGGATACT TCTACTTTGG TGAACTGCCC 
CTCTCACTCG CAGCTTGTAC CAACCAACCA GACATGGTGC ATTACCTGAC AGAGAATGGA 
CACAAGAAGG CGGATCTGAG GAGGCAGGAC TCAAGGGGAA ACACAGTCCT GCATGCTTTG 
GTCCACATTG CTGACAACAC TCGGGACAAC ACACGTTTTG TCACTAAGAT GTTTGACTTG 
CTGCTCATTA AATGTGCCAA ACTCTACCCG GATTGCAACC TGGAGAATAT ACTCAACAAC 
GACGGCATGT CCCCTCTCAT GATGGCCGCC AAACTGGGCA AAATTGGGGT GTTTCAGCAC 
ATCATTCGGA GAGAGATAAA AGATGAGGAA GCTCGACACC TCTCACGGAA ATTTAAAGAC 
TGGGCTTATG GACCAGTGTA CTCCAATCTG TATGATCTGT CCTCCCTGGA CACCTGCGGA 
GAGGAAGTGT CTGTTCTGGA AATACTCGTC TACAACAGCA AGATTGAGAA TCGGCATGAG 
ATGTTGGCGG TGGAACCAAT AAATGAACTG CTGAGGGCCA AGTGGCAGAA ATTTGCAGCG 
GTGACCTTCT ACATCAGTGT GTTTTCCTAT CTTGTGACTA TGATTATCTT CACTCTTGTG 
GCCTACTATC GCCCATCAGT GGGAAAACCC CCTTATGCTT ATGATACCAC AGAAGACAAG 
GTGCGTCTGG CTGGAGAGAT CATCACAGTG GGTTCTGGAC TCTTTTTCTT TGTAACAAAT 
ATTAAGGACC TTTTCCTGAA GAAGTGCCCC GGGGTGAATT CTATATTTGT TGATGGATCC 
TTTCAGCTTC TCTACTTCAT CTACTCTGTG TTGGTGCTGG TGAGCGCAGC GCTGTATCTG 
TCAGGCATTG AGGCCTACGT GTCTGTGATG GTGTTTGCTT TAACACTAGG CTGGATGAAC 
ACCCTCTATT TTACCAGAGG CCTTAAACTC ACTGGAACTT ACAGCATCAT GATCCAAAAG 
ATTCTCATCA AAGATTTGTT CCGGTTCCTG CTGGTCTACG TGCTCTTCAT GATTGGTTAT 
GCTTCAGCAT TAGTGTCGCT GTTGACCATC TGCCCTGATA AGGACACGTG TAAGGAAAAC 
TGTCCTACAT ATCCCGAATG TCGAGACACA AACACCTTCA GTGAATTCTT GCTGGACCTC 
TTCAAGCTGA CCATAGGCAT CGGAGACCTG GACAACATGC TGAAAGGCGC ACAGTATCCC 
GCCGTCTTCC TCATTCTGCT GGTCACCTAC ATTATCCTCA CCTTCGTCCT GTTGCTCAAC 
ATGTTAATCG CTCTGATGGG GGAGACGGTG GGACAGGTGT CCAAAGAAAG CAAGAAGATC 
TGGAAATTGC AGTGGGCGAC TACTATCCTG GACATTGAGC GTTCTTTCCC TGTGTGTCTG 
CGGCGGTCTT TCCGTGTGGG AGAGATGGTC ACTGTGGGCA AGGGTTTGGA TGGAAAGCCA 
GACAAACGCT GGTGCTTCAG GGTGGACGAG GTGAAGTGGT CCCACTGGAA TCAAAATCTG 
GGCATCATCA ATGAGGATCC AGGCCAGAAA GACCTTTCTG AACACACACA GGGTGGACGG 
GGCCTTCGAA GAGACCGCTG GTCCACTGTA GTACCGAGAG TGGTTGAACT GAATCGCGGC 
TCCAGAGATC ACACCGTAGA GATGGAGCCT CTCACAGGGA GACACCGACT CAAGTCTGAA 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Temperature-activated ion channels in neural crest cells confer maternal fever-associated birth defects.
Science signaling10(500)(2017 Oct)
Hutson MR,Keyte AL,Hern?ndez-Morales M,Gibbs E,Kupchinsky ZA,Argyridis I,Erwin KN,Pegram K,Kneifel M,Rosenberg PB,Matak P,Xie L,Grandl J,Davis EE,Katsanis N,Liu C,Benner EJ

Thermal stress in Danio rerio: a link between temperature, light, thermo-TRP channels, and clock genes.
Journal of thermal biology68(Pt A)128-138(2017 Aug)
Jer?nimo R,Moraes MN,de Assis LVM,Ramos BC,Rocha T,Castrucci AML

TRPV4-Mediated Detection of Hyposmotic Stress by Skin Keratinocytes Activates Developmental Immunity.
Journal of immunology (Baltimore, Md. : 1950)196(2)738-49(2016 Jan)
Galindo-Villegas J,Montalban-Arques A,Liarte S,de Oliveira S,Pardo-Pastor C,Rubio-Moscardo F,Meseguer J,Valverde MA,Mulero V

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trpv4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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