DROSHA gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010816832.2	XP_010815134.1	ribonuclease 3 isoform X1
XM_024981526.1	XP_024837294.1	ribonuclease 3 isoform X1
XM_005196186.3	XP_005196243.1	ribonuclease 3 isoform X1
XM_002696390.5	XP_002696436.1	ribonuclease 3 isoform X2
XM_015459113.2	XP_015314599.1	ribonuclease 3 isoform X2
XM_591998.9	XP_591998.4	ribonuclease 3 isoform X2
XM_015468377.1	XP_015323863.1	ribonuclease 3 isoform X2
XM_010816832.3	XP_010815134.1	ribonuclease 3 isoform X1
XM_005221630.3	XP_005221687.1	ribonuclease 3 isoform X1
XM_010824764.2	XP_010823066.1	ribonuclease 3 isoform X1
XM_005196187.3	XP_005196244.1	ribonuclease 3 isoform X3
XM_015459113.1	XP_015314599.1	ribonuclease 3 isoform X2
XM_005221631.3	XP_005221688.1	ribonuclease 3 isoform X3
XM_005221630.4	XP_005221687.1	ribonuclease 3 isoform X1
XM_002696390.6	XP_002696436.1	ribonuclease 3 isoform X2
XM_005221631.4	XP_005221688.1	ribonuclease 3 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa10628	XM_015468377.1 Latest version!	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa10628	XM_591998.9 Latest version!	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa10628	XM_002696390.6 Latest version!	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa10628	XM_015459113.2 Latest version!	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa10629	XM_005196187.3 Latest version!	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa10629	XM_005221631.4 Latest version!	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa10627	XM_005196186.3 Latest version!	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa10627	XM_010824764.2 Latest version!	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa10627	XM_005221630.4 Latest version!	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa10627	XM_010816832.3 Latest version!	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa10627	XM_024981526.1 Latest version!	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OBa10627	XM_010816832.2	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa10628	XM_015459113.1	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa10627	XM_005221630.3	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa10628	XM_002696390.5	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa10629	XM_005221631.3	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

DROSHA ( XM_002696390.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002696436.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa10628
Clone ID Related Accession (Same CDS sequence)	XM_002696390.5 , XM_015459113.2 , XM_591998.9 , XM_015468377.1 , XM_015459113.1 , XM_002696390.6
Accession Version	XM_002696390.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002696390.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT 
CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG 
GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT 
TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG 
AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC 
AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG 
CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG 
TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT 
AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC 
CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT 
TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG 
GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC 
GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG 
GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC 
AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC 
TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT 
ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA 
GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT 
GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA 
TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC 
CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA 
TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC 
ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC 
AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC 
ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG 
ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT 
TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT 
TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC 
ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA 
CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC 
AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT 
TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC 
TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG 
TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC 
CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT 
GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT 
GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002696436.1
CDS	179..4171
Translation	MSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_002696390.5
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002696390.5

ORF Insert Sequence:

ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT 
CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG 
GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT 
TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG 
AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC 
AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG 
CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG 
TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT 
AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC 
CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT 
TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG 
GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC 
GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG 
GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC 
AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC 
TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT 
ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA 
GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT 
GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA 
TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC 
CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA 
TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC 
ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC 
AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC 
ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG 
ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT 
TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT 
TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC 
ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA 
CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC 
AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT 
TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC 
TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG 
TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC 
CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT 
GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT 
GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_015459113.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314599.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa10628
Clone ID Related Accession (Same CDS sequence)	XM_002696390.5 , XM_015459113.2 , XM_591998.9 , XM_015468377.1 , XM_015459113.1 , XM_002696390.6
Accession Version	XM_015459113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT 
CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG 
GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT 
TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG 
AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC 
AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG 
CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG 
TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT 
AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC 
CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT 
TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG 
GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC 
GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG 
GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC 
AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC 
TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT 
ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA 
GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT 
GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA 
TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC 
CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA 
TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC 
ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC 
AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC 
ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG 
ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT 
TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT 
TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC 
ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA 
CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC 
AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT 
TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC 
TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG 
TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC 
CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT 
GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT 
GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314599.1
CDS	325..4317
Translation	MSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_015459113.2
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459113.2

ORF Insert Sequence:

ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT 
CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG 
GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT 
TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG 
AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC 
AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG 
CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG 
TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT 
AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC 
CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT 
TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG 
GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC 
GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG 
GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC 
AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC 
TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT 
ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA 
GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT 
GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA 
TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC 
CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA 
TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC 
ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC 
AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC 
ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG 
ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT 
TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT 
TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC 
ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA 
CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC 
AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT 
TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC 
TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG 
TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC 
CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT 
GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT 
GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_591998.9 ) cDNA ORF clone, Bos taurus(cattle) -> XP_591998.4 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.

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CloneID	OBa10628
Clone ID Related Accession (Same CDS sequence)	XM_002696390.5 , XM_015459113.2 , XM_591998.9 , XM_015468377.1 , XM_015459113.1 , XM_002696390.6
Accession Version	XM_591998.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_591998.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT 
CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG 
GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT 
TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG 
AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC 
AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG 
CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG 
TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT 
AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC 
CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT 
TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG 
GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC 
GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG 
GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC 
AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC 
TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT 
ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA 
GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT 
GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA 
TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC 
CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA 
TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC 
ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC 
AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC 
ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG 
ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT 
TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT 
TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC 
ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA 
CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC 
AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT 
TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC 
TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG 
TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC 
CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT 
GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT 
GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_591998.4
CDS	179..4171
Translation	MSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_591998.9
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_591998.9

ORF Insert Sequence:

ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT 
CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG 
GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT 
TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG 
AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC 
AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG 
CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG 
TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT 
AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC 
CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT 
TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG 
GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC 
GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG 
GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC 
AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC 
TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT 
ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA 
GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT 
GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA 
TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC 
CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA 
TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC 
ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC 
AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC 
ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG 
ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT 
TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT 
TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC 
ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA 
CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC 
AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT 
TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC 
TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG 
TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC 
CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT 
GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT 
GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_015468377.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323863.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa10628
Clone ID Related Accession (Same CDS sequence)	XM_002696390.5 , XM_015459113.2 , XM_591998.9 , XM_015468377.1 , XM_015459113.1 , XM_002696390.6
Accession Version	XM_015468377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT 
CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG 
GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT 
TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG 
AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC 
AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG 
CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG 
TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT 
AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC 
CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT 
TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG 
GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC 
GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG 
GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC 
AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC 
TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT 
ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA 
GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT 
GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA 
TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC 
CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA 
TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC 
ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC 
AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC 
ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG 
ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT 
TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT 
TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC 
ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA 
CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC 
AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT 
TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC 
TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG 
TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC 
CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT 
GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT 
GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323863.1
CDS	307..4299
Translation	MSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_015468377.1
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468377.1

ORF Insert Sequence:

ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT 
CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG 
GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT 
TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG 
AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC 
AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG 
CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG 
TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT 
AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC 
CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT 
TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG 
GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC 
GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG 
GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC 
AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC 
TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT 
ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA 
GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT 
GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA 
TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC 
CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA 
TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC 
ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC 
AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC 
ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG 
ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT 
TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT 
TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC 
ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA 
CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC 
AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT 
TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC 
TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG 
TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC 
CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT 
GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT 
GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_015459113.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314599.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa10628
Clone ID Related Accession (Same CDS sequence)	XM_002696390.5 , XM_015459113.2 , XM_591998.9 , XM_015468377.1 , XM_015459113.1 , XM_002696390.6
Accession Version	XM_015459113.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT 
CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG 
GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT 
TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG 
AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC 
AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG 
CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG 
TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT 
AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC 
CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT 
TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG 
GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC 
GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG 
GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC 
AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC 
TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT 
ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA 
GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT 
GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA 
TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC 
CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA 
TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC 
ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC 
AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC 
ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG 
ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT 
TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT 
TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC 
ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA 
CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC 
AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT 
TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC 
TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG 
TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC 
CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT 
GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT 
GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314599.1
CDS	307..4299
Translation	MSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_015459113.1
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459113.1

ORF Insert Sequence:

ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT 
CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG 
GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT 
TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG 
AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC 
AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG 
CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG 
TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT 
AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC 
CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT 
TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG 
GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC 
GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG 
GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC 
AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC 
TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT 
ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA 
GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT 
GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA 
TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC 
CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA 
TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC 
ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC 
AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC 
ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG 
ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT 
TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT 
TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC 
ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA 
CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC 
AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT 
TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC 
TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG 
TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC 
CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT 
GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT 
GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_002696390.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002696436.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA.

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CloneID	OBa10628
Clone ID Related Accession (Same CDS sequence)	XM_002696390.5 , XM_015459113.2 , XM_591998.9 , XM_015468377.1 , XM_015459113.1 , XM_002696390.6
Accession Version	XM_002696390.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002696390.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT 
CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG 
GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT 
TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG 
AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC 
AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG 
CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG 
TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT 
AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC 
CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT 
TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG 
GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC 
GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG 
GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC 
AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC 
TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT 
ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA 
GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT 
GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA 
TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC 
CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA 
TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC 
ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC 
AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC 
ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG 
ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT 
TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT 
TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC 
ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA 
CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC 
AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT 
TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC 
TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG 
TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC 
CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT 
GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT 
GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002696436.1
CDS	197..4189
Translation	MSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_002696390.6
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002696390.6

ORF Insert Sequence:

ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT 
CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG 
GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT 
TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG 
AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC 
AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG 
CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG 
TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT 
AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC 
CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT 
TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG 
GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC 
GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG 
GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC 
AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC 
TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT 
ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA 
GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT 
GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA 
TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC 
CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA 
TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC 
ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC 
AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC 
ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG 
ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT 
TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT 
TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC 
ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA 
CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC 
AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT 
TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC 
TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG 
TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC 
CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT 
GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT 
GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_005196187.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196244.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA.

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CloneID	OBa10629
Clone ID Related Accession (Same CDS sequence)	XM_005196187.3 , XM_005221631.3 , XM_005221631.4
Accession Version	XM_005196187.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196187.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196244.1
CDS	221..4213
Translation	MQGSACHRMSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_005196187.3
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196187.3

ORF Insert Sequence:

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_005221631.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221688.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa10629
Clone ID Related Accession (Same CDS sequence)	XM_005196187.3 , XM_005221631.3 , XM_005221631.4
Accession Version	XM_005221631.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221631.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221688.1
CDS	221..4213
Translation	MQGSACHRMSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_005221631.3
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221631.3

ORF Insert Sequence:

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_005221631.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221688.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa10629
Clone ID Related Accession (Same CDS sequence)	XM_005196187.3 , XM_005221631.3 , XM_005221631.4
Accession Version	XM_005221631.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221631.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221688.1
CDS	240..4232
Translation	MQGSACHRMSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_005221631.4
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221631.4

ORF Insert Sequence:

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA 
GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG 
CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC 
CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA 
GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA 
TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA 
AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG 
CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC 
TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC 
AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG 
TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT 
CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT 
GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT 
GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT 
GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC 
CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA 
CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG 
GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG 
AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT 
CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC 
AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC 
AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT 
ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT 
TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG 
CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC 
AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC 
AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA 
GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT 
GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG 
GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_010816832.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815134.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.

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CloneID	OBa10627
Clone ID Related Accession (Same CDS sequence)	XM_010816832.2 , XM_024981526.1 , XM_005196186.3 , XM_010816832.3 , XM_005221630.3 , XM_010824764.2 , XM_005221630.4
Accession Version	XM_010816832.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816832.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT 
TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG 
AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA 
GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC 
TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA 
ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC 
ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT 
TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC 
ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC 
CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT 
GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA 
GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT 
GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT 
CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC 
CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA 
GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA 
CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA 
ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC 
CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG 
GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG 
TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG 
CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC 
TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC 
GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG 
GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA 
AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG 
ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT 
ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG 
ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG 
TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815134.1
CDS	349..4365
Translation	MQGSACHRMSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_010816832.2
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816832.2

ORF Insert Sequence:

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT 
TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG 
AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA 
GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC 
TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA 
ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC 
ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT 
TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC 
ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC 
CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT 
GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA 
GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT 
GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT 
CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC 
CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA 
GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA 
CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA 
ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC 
CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG 
GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG 
TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG 
CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC 
TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC 
GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG 
GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA 
AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG 
ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT 
ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG 
ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG 
TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_024981526.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837294.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa10627
Clone ID Related Accession (Same CDS sequence)	XM_010816832.2 , XM_024981526.1 , XM_005196186.3 , XM_010816832.3 , XM_005221630.3 , XM_010824764.2 , XM_005221630.4
Accession Version	XM_024981526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT 
TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG 
AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA 
GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC 
TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA 
ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC 
ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT 
TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC 
ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC 
CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT 
GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA 
GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT 
GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT 
CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC 
CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA 
GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA 
CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA 
ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC 
CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG 
GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG 
TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG 
CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC 
TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC 
GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG 
GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA 
AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG 
ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT 
ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG 
ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG 
TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837294.1
CDS	77..4093
Translation	MQGSACHRMSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_024981526.1
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981526.1

ORF Insert Sequence:

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT 
TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG 
AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA 
GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC 
TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA 
ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC 
ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT 
TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC 
ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC 
CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT 
GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA 
GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT 
GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT 
CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC 
CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA 
GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA 
CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA 
ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC 
CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG 
GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG 
TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG 
CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC 
TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC 
GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG 
GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA 
AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG 
ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT 
ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG 
ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG 
TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_005196186.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196243.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa10627
Clone ID Related Accession (Same CDS sequence)	XM_010816832.2 , XM_024981526.1 , XM_005196186.3 , XM_010816832.3 , XM_005221630.3 , XM_010824764.2 , XM_005221630.4
Accession Version	XM_005196186.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196186.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT 
TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG 
AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA 
GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC 
TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA 
ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC 
ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT 
TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC 
ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC 
CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT 
GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA 
GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT 
GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT 
CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC 
CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA 
GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA 
CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA 
ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC 
CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG 
GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG 
TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG 
CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC 
TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC 
GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG 
GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA 
AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG 
ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT 
ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG 
ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG 
TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196243.1
CDS	221..4237
Translation	MQGSACHRMSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_005196186.3
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196186.3

ORF Insert Sequence:

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT 
TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG 
AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA 
GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC 
TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA 
ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC 
ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT 
TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC 
ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC 
CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT 
GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA 
GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT 
GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT 
CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC 
CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA 
GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA 
CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA 
ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC 
CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG 
GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG 
TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG 
CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC 
TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC 
GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG 
GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA 
AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG 
ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT 
ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG 
ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG 
TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_010816832.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815134.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.

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CloneID	OBa10627
Clone ID Related Accession (Same CDS sequence)	XM_010816832.2 , XM_024981526.1 , XM_005196186.3 , XM_010816832.3 , XM_005221630.3 , XM_010824764.2 , XM_005221630.4
Accession Version	XM_010816832.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT 
TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG 
AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA 
GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC 
TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA 
ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC 
ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT 
TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC 
ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC 
CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT 
GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA 
GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT 
GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT 
CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC 
CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA 
GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA 
CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA 
ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC 
CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG 
GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG 
TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG 
CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC 
TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC 
GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG 
GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA 
AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG 
ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT 
ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG 
ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG 
TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815134.1
CDS	367..4383
Translation	MQGSACHRMSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_010816832.3
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816832.3

ORF Insert Sequence:

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT 
TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG 
AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA 
GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC 
TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA 
ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC 
ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT 
TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC 
ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC 
CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT 
GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA 
GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT 
GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT 
CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC 
CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA 
GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA 
CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA 
ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC 
CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG 
GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG 
TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG 
CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC 
TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC 
GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG 
GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA 
AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG 
ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT 
ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG 
ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG 
TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_005221630.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221687.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa10627
Clone ID Related Accession (Same CDS sequence)	XM_010816832.2 , XM_024981526.1 , XM_005196186.3 , XM_010816832.3 , XM_005221630.3 , XM_010824764.2 , XM_005221630.4
Accession Version	XM_005221630.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221630.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT 
TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG 
AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA 
GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC 
TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA 
ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC 
ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT 
TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC 
ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC 
CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT 
GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA 
GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT 
GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT 
CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC 
CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA 
GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA 
CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA 
ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC 
CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG 
GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG 
TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG 
CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC 
TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC 
GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG 
GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA 
AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG 
ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT 
ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG 
ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG 
TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221687.1
CDS	221..4237
Translation	MQGSACHRMSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_005221630.3
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221630.3

ORF Insert Sequence:

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT 
TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG 
AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA 
GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC 
TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA 
ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC 
ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT 
TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC 
ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC 
CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT 
GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA 
GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT 
GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT 
CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC 
CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA 
GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA 
CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA 
ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC 
CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG 
GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG 
TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG 
CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC 
TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC 
GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG 
GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA 
AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG 
ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT 
ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG 
ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG 
TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_010824764.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823066.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa10627
Clone ID Related Accession (Same CDS sequence)	XM_010816832.2 , XM_024981526.1 , XM_005196186.3 , XM_010816832.3 , XM_005221630.3 , XM_010824764.2 , XM_005221630.4
Accession Version	XM_010824764.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824764.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT 
TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG 
AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA 
GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC 
TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA 
ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC 
ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT 
TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC 
ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC 
CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT 
GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA 
GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT 
GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT 
CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC 
CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA 
GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA 
CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA 
ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC 
CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG 
GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG 
TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG 
CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC 
TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC 
GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG 
GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA 
AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG 
ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT 
ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG 
ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG 
TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823066.1
CDS	349..4365
Translation	MQGSACHRMSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_010824764.2
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824764.2

ORF Insert Sequence:

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT 
TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG 
AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA 
GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC 
TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA 
ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC 
ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT 
TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC 
ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC 
CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT 
GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA 
GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT 
GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT 
CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC 
CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA 
GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA 
CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA 
ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC 
CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG 
GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG 
TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG 
CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC 
TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC 
GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG 
GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA 
AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG 
ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT 
ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG 
ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG 
TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_005221630.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221687.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.

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CloneID	OBa10627
Clone ID Related Accession (Same CDS sequence)	XM_010816832.2 , XM_024981526.1 , XM_005196186.3 , XM_010816832.3 , XM_005221630.3 , XM_010824764.2 , XM_005221630.4
Accession Version	XM_005221630.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ribonuclease 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221630.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT 
TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG 
AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA 
GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC 
TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA 
ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC 
ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT 
TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC 
ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC 
CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT 
GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA 
GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT 
GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT 
CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC 
CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA 
GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA 
CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA 
ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC 
CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG 
GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG 
TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG 
CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC 
TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC 
GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG 
GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA 
AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG 
ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT 
ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG 
ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG 
TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221687.1
CDS	239..4255
Translation	MQGSACHRMSFHPGGPRCPRGRGGHGARPSSAPAFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRPPFPVPPCFPPMPPPLPCPNNPPVPGAPPGQGAFPFMMPPPSLPHPPPPPVVPQQVNYQYPPGYSHHSFPPPNFNSFQNSPSSFPPSASNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPDRRRQDSRYRSDHERGRTPSRHRSYERSRERERERHRHRDSRRSPSLERSYKKEYKRSGSRSPSREKKRGRWEEEKDRWSDIPGSGKEKNYTSIKEKEPEEGLPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGAKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEDAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEKEPDETEDIKK

Target ORF information:

RefSeq Version	XM_005221630.4
Organism	Bos taurus(cattle)
Definition	Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221630.4

ORF Insert Sequence:

ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG 
AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT 
TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA 
CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG 
CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC 
ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA 
GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC 
GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC 
CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC 
AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG 
CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG 
AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA 
AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG 
AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA 
TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA 
AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG 
GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG 
CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC 
CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT 
GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA 
GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT 
TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC 
TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC 
CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC 
AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG 
GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT 
ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT 
CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA 
CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG 
CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT 
TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT 
CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA 
CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA 
GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG 
TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA 
CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG 
ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT 
TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG 
AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA 
GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC 
TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA 
ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC 
ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT 
TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC 
ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC 
CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT 
GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA 
GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT 
GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT 
CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC 
CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA 
GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA 
CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA 
ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC 
CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG 
GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG 
TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG 
CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC 
TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC 
GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG 
GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA 
AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG 
ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT 
ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG 
ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG 
TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DROSHA
Entrez Gene ID	540168
Full Name	drosha ribonuclease III
Synonyms	RNASEN
General protein information	Preferred Names drosha ribonuclease III Names ribonuclease 3 drosha, ribonuclease type III ribonuclease type III, nuclear
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	DROSHA	NP_037367.3
Mus musculus (house mouse)	Drosha	NP_001123621.1
Rattus norvegicus (Norway rat)	Drosha	NP_001101125.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008087	XP_555381.3
	DROSHA	XP_002804372.1
Caenorhabditis elegans (roundworm)	drsh-1	NP_492599.1
Xenopus tropicalis (tropical clawed frog)	drosha	NP_001107152.1
Pan troglodytes (chimpanzee)	DROSHA	XP_001157472.2
Bos taurus (cattle)	DROSHA	XP_005221686.1
Canis lupus familiaris (dog)	DROSHA	XP_854135.2
Gallus gallus (chicken)	DROSHA	NP_001006379.1
Danio rerio (zebrafish)	rnasen	NP_001103942.1
Drosophila melanogaster (fruit fly)	drosha	NP_477436.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DROSHA cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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