DROSHA cDNA ORF clone, Homo sapiens(human)

The following DROSHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DROSHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu105662 XM_024446036.1
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu95850 XM_017009402.1
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu95847 XM_017009399.2
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu95848 XM_017009400.2
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95849 XM_017009401.2
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu39316 XM_005248294.4
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu67332 XM_011514033.3
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu16375 NM_001100412.2
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu16612 NM_013235.5
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu16375 XM_024446035.1
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu16612 XM_005248291.4
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu16612 XM_024446034.1
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu16612 XM_024446033.1
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu39314 XM_005248292.4
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu39315 XM_005248293.4
Latest version!
Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OHu16612 XM_005248291.3 Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu39316 XM_005248294.3 Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu16375 NM_001100412.1 Homo sapiens drosha ribonuclease III (DROSHA), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu95847 XM_017009399.1 Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu95848 XM_017009400.1 Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu16612 NM_013235.4 Homo sapiens drosha ribonuclease III (DROSHA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu39314 XM_005248292.3 Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu67332 XM_011514033.2 Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu39315 XM_005248293.3 Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu95849 XM_017009401.1 Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu105662
    Clone ID Related Accession (Same CDS sequence) XM_024446036.1
    Accession Version XM_024446036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024301804.1
    CDS403..3348
    Translation

    Target ORF information:

    RefSeq Version XM_024446036.1
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024446036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95850
    Clone ID Related Accession (Same CDS sequence) XM_017009402.1
    Accession Version XM_017009402.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTACCTCT GTATTAATAC TCCACTCTCA CATTTTAGAT TGCCTTTTGA AATGAACCTC 
    CTCGATGTTT CCTCAGATAC TTTACCCTCA ACCTGTGTGA AACTAGACTC GTCATCTTCC
    CTGCATATTG AATTTGAGAT TCCACTGTGT AAAGTAATTA GATTCAACAT AGACTACACG
    ATTCATTTCA TTGAAGAGAT GATGCCGGAG AATTTTTGTG TGAAAGGGCT TGAACTCTTT
    TCACTGTTCC TATTCAGAGA TATTTTGGAA TTATATGACT GGAATCTTAA AGGTCCTTTG
    TTTGAAGACA GCCCTCCCTG CTGCCCAAGA TTTCATTTCA TGCCACGTTT TGTAAGATTT
    CTTCCAGATG GAGGAAAGGA AGTGCTGTCC ATGCACCAGA TTCTCCTGTA CTTGTTAAGG
    TGCAGCAAAG CCCTGGTGCC TGAGGAGGAG ATTGCCAATA TGCTTCAGTG GGAGGAGCTG
    GAGTGGCAGA AATATGCAGA AGAATGCAAA GGCATGATTG TTACCAACCC TGGGACGAAA
    CCAAGCTCTG TCCGTATCGA TCAACTGGAT CGTGAACAGT TCAACCCCGA TGTGATTACT
    TTTCCGATTA TCGTCCACTT TGGGATACGC CCTGCACAGT TGAGTTATGC AGGAGACCCA
    CAGTACCAAA AACTGTGGAA GAGTTATGTG AAACTTCGCC ACCTCCTAGC AAATAGTCCC
    AAAGTCAAAC AAACTGACAA ACAGAAGCTG GCACAGAGGG AGGAAGCCCT CCAAAAAATA
    CGGCAGAAGA ATACAATGAG ACGAGAAGTA ACGGTGGAGC TAAGTAGCCA AGGATTCTGG
    AAAACTGGCA TCCGTTCTGA TGTCTGTCAG CATGCAATGA TGCTACCTGT TCTGACCCAT
    CATATCCGCT ACCACCAATG CCTAATGCAT TTGGACAAGT TGATAGGATA TACTTTCCAA
    GATCGTTGTC TGTTGCAGCT GGCCATGACT CATCCAAGTC ATCATTTAAA TTTTGGAATG
    AATCCTGATC ATGCCAGGAA TTCATTATCT AACTGTGGAA TTCGGCAGCC CAAATACGGA
    GACAGAAAAG TTCATCACAT GCACATGCGG AAGAAAGGGA TTAACACCTT GATAAATATC
    ATGTCACGCC TTGGCCAAGA TGACCCAACT CCCTCGAGGA TTAACCACAA TGAACGGTTG
    GAATTCCTGG GTGATGCTGT TGTTGAATTT CTGACCAGCG TCCATTTGTA CTATTTGTTT
    CCTAGTCTGG AAGAAGGAGG ATTAGCAACC TATCGGACTG CCATTGTTCA GAATCAGCAC
    CTTGCCATGC TAGCAAAGAA ACTTGAACTG GATCGATTTA TGCTGTATGC TCACGGGCCT
    GACCTTTGTA GAGAATCGGA CCTTCGACAT GCAATGGCCA ATTGTTTTGA AGCGTTAATA
    GGAGCTGTTT ACTTGGAGGG AAGCCTGGAG GAAGCCAAGC AGTTATTTGG ACGCTTGCTC
    TTTAATGATC CGGACCTGCG CGAAGTCTGG CTCAATTATC CTCTCCACCC ACTCCAACTA
    CAAGAGCCAA ATACTGATCG ACAACTTATT GAAACTTCTC CAGTTCTACA AAAACTTACT
    GAGTTTGAAG AAGCAATTGG AGTAATTTTT ACTCATGTTC GACTTCTGGC AAGGGCATTC
    ACATTGAGAA CTGTGGGATT TAACCATCTG ACCCTAGGCC ACAATCAGAG AATGGAATTC
    CTAGGTGACT CCATAATGCA ACTGGTAGCC ACAGAGTACT TATTCATTCA TTTCCCAGAT
    CATCATGAAG GACACTTAAC TTTGTTGCGA AGCTCTTTGG TGAATAATAG AACTCAGGCC
    AAGGTAGCGG AGGAGCTGGG CATGCAGGAG TACGCCATAA CCAACGACAA GACCAAGAGG
    CCTGTGGCGC TTCGCACCAA GACCTTGGCG GACCTTTTGG AATCATTTAT TGCAGCGCTG
    TACATTGATA AGGATTTGGA ATATGTTCAT ACTTTCATGA ATGTCTGCTT CTTTCCACGA
    TTGAAAGAGT TCATTTTGAA TCAGGATTGG AATGACCCCA AATCCCAGCT TCAGCAGTGT
    TGCTTGACAC TTAGGACAGA AGGAAAAGAG CCAGACATTC CTCTGTACAA GACTCTGCAG
    ACAGTGGGCC CATCCCATGC CCGAACCTAC ACTGTGGCTG TTTATTTCAA GGGAGAAAGA
    ATAGGCTGTG GGAAAGGACC AAGTATTCAG CAAGCGGAAA TGGGAGCAGC AATGGATGCG
    CTTGAAAAAT ATAATTTTCC CCAGATGGCC CATCAGAAGC GGTTCATCGA ACGGAAGTAC
    AGACAAGAGT TAAAAGAAAT GAGGTGGGAA AGAGAGCATC AAGAGAGAGA GCCAGATGAG
    ACTGAAGACA TCAAGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864891.1
    CDS155..2575
    Translation

    Target ORF information:

    RefSeq Version XM_017009402.1
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009402.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTACCTCT GTATTAATAC TCCACTCTCA CATTTTAGAT TGCCTTTTGA AATGAACCTC 
    CTCGATGTTT CCTCAGATAC TTTACCCTCA ACCTGTGTGA AACTAGACTC GTCATCTTCC
    CTGCATATTG AATTTGAGAT TCCACTGTGT AAAGTAATTA GATTCAACAT AGACTACACG
    ATTCATTTCA TTGAAGAGAT GATGCCGGAG AATTTTTGTG TGAAAGGGCT TGAACTCTTT
    TCACTGTTCC TATTCAGAGA TATTTTGGAA TTATATGACT GGAATCTTAA AGGTCCTTTG
    TTTGAAGACA GCCCTCCCTG CTGCCCAAGA TTTCATTTCA TGCCACGTTT TGTAAGATTT
    CTTCCAGATG GAGGAAAGGA AGTGCTGTCC ATGCACCAGA TTCTCCTGTA CTTGTTAAGG
    TGCAGCAAAG CCCTGGTGCC TGAGGAGGAG ATTGCCAATA TGCTTCAGTG GGAGGAGCTG
    GAGTGGCAGA AATATGCAGA AGAATGCAAA GGCATGATTG TTACCAACCC TGGGACGAAA
    CCAAGCTCTG TCCGTATCGA TCAACTGGAT CGTGAACAGT TCAACCCCGA TGTGATTACT
    TTTCCGATTA TCGTCCACTT TGGGATACGC CCTGCACAGT TGAGTTATGC AGGAGACCCA
    CAGTACCAAA AACTGTGGAA GAGTTATGTG AAACTTCGCC ACCTCCTAGC AAATAGTCCC
    AAAGTCAAAC AAACTGACAA ACAGAAGCTG GCACAGAGGG AGGAAGCCCT CCAAAAAATA
    CGGCAGAAGA ATACAATGAG ACGAGAAGTA ACGGTGGAGC TAAGTAGCCA AGGATTCTGG
    AAAACTGGCA TCCGTTCTGA TGTCTGTCAG CATGCAATGA TGCTACCTGT TCTGACCCAT
    CATATCCGCT ACCACCAATG CCTAATGCAT TTGGACAAGT TGATAGGATA TACTTTCCAA
    GATCGTTGTC TGTTGCAGCT GGCCATGACT CATCCAAGTC ATCATTTAAA TTTTGGAATG
    AATCCTGATC ATGCCAGGAA TTCATTATCT AACTGTGGAA TTCGGCAGCC CAAATACGGA
    GACAGAAAAG TTCATCACAT GCACATGCGG AAGAAAGGGA TTAACACCTT GATAAATATC
    ATGTCACGCC TTGGCCAAGA TGACCCAACT CCCTCGAGGA TTAACCACAA TGAACGGTTG
    GAATTCCTGG GTGATGCTGT TGTTGAATTT CTGACCAGCG TCCATTTGTA CTATTTGTTT
    CCTAGTCTGG AAGAAGGAGG ATTAGCAACC TATCGGACTG CCATTGTTCA GAATCAGCAC
    CTTGCCATGC TAGCAAAGAA ACTTGAACTG GATCGATTTA TGCTGTATGC TCACGGGCCT
    GACCTTTGTA GAGAATCGGA CCTTCGACAT GCAATGGCCA ATTGTTTTGA AGCGTTAATA
    GGAGCTGTTT ACTTGGAGGG AAGCCTGGAG GAAGCCAAGC AGTTATTTGG ACGCTTGCTC
    TTTAATGATC CGGACCTGCG CGAAGTCTGG CTCAATTATC CTCTCCACCC ACTCCAACTA
    CAAGAGCCAA ATACTGATCG ACAACTTATT GAAACTTCTC CAGTTCTACA AAAACTTACT
    GAGTTTGAAG AAGCAATTGG AGTAATTTTT ACTCATGTTC GACTTCTGGC AAGGGCATTC
    ACATTGAGAA CTGTGGGATT TAACCATCTG ACCCTAGGCC ACAATCAGAG AATGGAATTC
    CTAGGTGACT CCATAATGCA ACTGGTAGCC ACAGAGTACT TATTCATTCA TTTCCCAGAT
    CATCATGAAG GACACTTAAC TTTGTTGCGA AGCTCTTTGG TGAATAATAG AACTCAGGCC
    AAGGTAGCGG AGGAGCTGGG CATGCAGGAG TACGCCATAA CCAACGACAA GACCAAGAGG
    CCTGTGGCGC TTCGCACCAA GACCTTGGCG GACCTTTTGG AATCATTTAT TGCAGCGCTG
    TACATTGATA AGGATTTGGA ATATGTTCAT ACTTTCATGA ATGTCTGCTT CTTTCCACGA
    TTGAAAGAGT TCATTTTGAA TCAGGATTGG AATGACCCCA AATCCCAGCT TCAGCAGTGT
    TGCTTGACAC TTAGGACAGA AGGAAAAGAG CCAGACATTC CTCTGTACAA GACTCTGCAG
    ACAGTGGGCC CATCCCATGC CCGAACCTAC ACTGTGGCTG TTTATTTCAA GGGAGAAAGA
    ATAGGCTGTG GGAAAGGACC AAGTATTCAG CAAGCGGAAA TGGGAGCAGC AATGGATGCG
    CTTGAAAAAT ATAATTTTCC CCAGATGGCC CATCAGAAGC GGTTCATCGA ACGGAAGTAC
    AGACAAGAGT TAAAAGAAAT GAGGTGGGAA AGAGAGCATC AAGAGAGAGA GCCAGATGAG
    ACTGAAGACA TCAAGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95847
    Clone ID Related Accession (Same CDS sequence) XM_017009399.1 , XM_017009399.2
    Accession Version XM_017009399.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGGAGTTA CGGTTTATCG GTTGTTCCTG AACCTGCTGG ATGCACACCA
    GAATTACCTG GGGAGATTAT TAAAAATACA GATTCTTGGG CCCCACCCCT GGAGATTGTG
    AATCATCGCT CCCCAAGTAG GGAGAAGAAG AGAGCTCGTT GGGAGGAAGA AAAAGACCGT
    TGGAGTGACA ACCAGAGTTC TGGCAAAGAC AAGAACTATA CCTCAATCAA GGAAAAAGAG
    CCCGAGGAGA CCATGCCTGA CAAGAATGAG GAGGAAGAAG AAGAACTTCT TAAGCCTGTG
    TGGATTCGAT GCACTCATTC AGAAAACTAC TACTCCAGTG ACCCCATGGA TCAGGTGGGA
    GATTCTACAG TGGTTGGAAC GAGTAGGCTT CGTGACTTAT ATGACAAATT TGAGGAGGAG
    TTGGGGAGCA GGCAAGAAAA GGCCAAAGCT GCTCGGCCTC CGTGGGAACC TCCAAAGACG
    AAGCTCGATG AAGATTTAGA GAGTTCCAGT GAATCCGAGT GTGAGTCTGA TGAGGACAGC
    ACCTGTTCTA GCAGCTCAGA CTCTGAAGTT TTTGACGTTA TTGCAGAAAT CAAACGCAAA
    AAGGCCCACC CTGACCGACT TCATGATGAA CTTTGGTACA ACGATCCAGG CCAGATGAAT
    GATGGACCAC TCTGCAAATG CAGCGCAAAG GCAAGACGCA CAGGAATTAG GCACAGCATT
    TATCCTGGAG AAGAGGCCAT CAAGCCCTGT CGTCCTATGA CCAACAATGC TGGCAGACTT
    TTCCACTACC GGATCACAGT CTCCCCGCCT ACGAACTTTT TAACTGACAG GCCAACTGTT
    ATAGAATACG ATGATCACGA GTATATCTTT GAAGGATTTT CTATGTTTGC ACATGCCCCC
    CTGACCAATA TTCCACTGTG TAAAGTAATT AGATTCAACA TAGACTACAC GATTCATTTC
    ATTGAAGAGA TGATGCCGGA GAATTTTTGT GTGAAAGGGC TTGAACTCTT TTCACTGTTC
    CTATTCAGAG ATATTTTGGA ATTATATGAC TGGAATCTTA AAGGTCCTTT GTTTGAAGAC
    AGCCCTCCCT GCTGCCCAAG ATTTCATTTC ATGCCACGTT TTGTAAGATT TCTTCCAGAT
    GGAGGAAAGG AAGTGCTGTC CATGCACCAG ATTCTCCTGT ACTTGTTAAG GTGCAGCAAA
    GCCCTGGTGC CTGAGGAGGA GATTGCCAAT ATGCTTCAGT GGGAGGAGCT GGAGTGGCAG
    AAATATGCAG AAGAATGCAA AGGCATGATT GTTACCAACC CTGGGACGAA ACCAAGCTCT
    GTCCGTATCG ATCAACTGGA TCGTGAACAG TTCAACCCCG ATGTGATTAC TTTTCCGATT
    ATCGTCCACT TTGGGATACG CCCTGCACAG TACCAAAAAC TGTGGAAGAG TTATGTGAAA
    CTTCGCCACC TCCTAGCAAA TAGTCCCAAA GTCAAACAAA CTGACAAACA GAAGCTGGCA
    CAGAGGGAGG AAGCCCTCCA AAAAATACGG CAGAAGAATA CAATGAGACG AGAAGTAACG
    GTGGAGCTAA GTAGCCAAGG ATTCTGGAAA ACTGGCATCC GTTCTGATGT CTGTCAGCAT
    GCAATGATGC TACCTGTTCT GACCCATCAT ATCCGCTACC ACCAATGCCT AATGCATTTG
    GACAAGTTGA TAGGATATAC TTTCCAAGAT CGTTGTCTGT TGCAGCTGGC CATGACTCAT
    CCAAGTCATC ATTTAAATTT TGGAATGAAT CCTGATCATG CCAGGAATTC ATTATCTAAC
    TGTGGAATTC GGCAGCCCAA ATACGGAGAC AGAAAAGTTC ATCACATGCA CATGCGGAAG
    AAAGGGATTA ACACCTTGAT AAATATCATG TCACGCCTTG GCCAAGATGA CCCAACTCCC
    TCGAGGATTA ACCACAATGA ACGGTTGGAA TTCCTGGGTG ATGCTGTTGT TGAATTTCTG
    ACCAGCGTCC ATTTGTACTA TTTGTTTCCT AGTCTGGAAG AAGGAGGATT AGCAACCTAT
    CGGACTGCCA TTGTTCAGAA TCAGCACCTT GCCATGCTAG CAAAGAAACT TGAACTGGAT
    CGATTTATGC TGTATGCTCA CGGGCCTGAC CTTTGTAGAG AATCGGACCT TCGACATGCA
    ATGGCCAATT GTTTTGAAGC GTTAATAGGA GCTGTTTACT TGGAGGGAAG CCTGGAGGAA
    GCCAAGCAGT TATTTGGACG CTTGCTCTTT AATGATCCGG ACCTGCGCGA AGTCTGGCTC
    AATTATCCTC TCCACCCACT CCAACTACAA GAGCCAAATA CTGATCGACA ACTTATTGAA
    ACTTCTCCAG TTCTACAAAA ACTTACTGAG TTTGAAGAAG CAATTGGAGT AATTTTTACT
    CATGTTCGAC TTCTGGCAAG GGCATTCACA TTGAGAACTG TGGGATTTAA CCATCTGACC
    CTAGGCCACA ATCAGAGAAT GGAATTCCTA GGTGACTCCA TAATGCAACT GGTAGCCACA
    GAGTACTTAT TCATTCATTT CCCAGATCAT CATGAAGGAC ACTTAACTTT GTTGCGAAGC
    TCTTTGGTGA ATAATAGAAC TCAGGCCAAG GTAGCGGAGG AGCTGGGCAT GCAGGAGTAC
    GCCATAACCA ACGACAAGAC CAAGAGGCCT GTGGCGCTTC GCACCAAGAC CTTGGCGGAC
    CTTTTGGAAT CATTTATTGC AGCGCTGTAC ATTGATAAGG ATTTGGAATA TGTTCATACT
    TTCATGAATG TCTGCTTCTT TCCACGATTG AAAGAGTTCA TTTTGAATCA GGATTGGAAT
    GACCCCAAAT CCCAGCTTCA GCAGTGTTGC TTGACACTTA GGACAGAAGG AAAAGAGCCA
    GACATTCCTC TGTACAAGAC TCTGCAGACA GTGGGCCCAT CCCATGCCCG AACCTACACT
    GTGGCTGTTT ATTTCAAGGG AGAAAGAATA GGCTGTGGGA AAGGACCAAG TATTCAGCAA
    GCGGAAATGG GAGCAGCAAT GGATGCGCTT GAAAAATATA ATTTTCCCCA GATGGCCCAT
    CAGAAGCGGT TCATCGAACG GAAGTACAGA CAAGAGTTAA AAGAAATGAG GTGGGAAAGA
    GAGCATCAAG AGAGAGAGCC AGATGAGACT GAAGACATCA AGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864888.1
    CDS436..4443
    Translation

    Target ORF information:

    RefSeq Version XM_017009399.1
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009399.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGGAGTTA CGGTTTATCG GTTGTTCCTG AACCTGCTGG ATGCACACCA
    GAATTACCTG GGGAGATTAT TAAAAATACA GATTCTTGGG CCCCACCCCT GGAGATTGTG
    AATCATCGCT CCCCAAGTAG GGAGAAGAAG AGAGCTCGTT GGGAGGAAGA AAAAGACCGT
    TGGAGTGACA ACCAGAGTTC TGGCAAAGAC AAGAACTATA CCTCAATCAA GGAAAAAGAG
    CCCGAGGAGA CCATGCCTGA CAAGAATGAG GAGGAAGAAG AAGAACTTCT TAAGCCTGTG
    TGGATTCGAT GCACTCATTC AGAAAACTAC TACTCCAGTG ACCCCATGGA TCAGGTGGGA
    GATTCTACAG TGGTTGGAAC GAGTAGGCTT CGTGACTTAT ATGACAAATT TGAGGAGGAG
    TTGGGGAGCA GGCAAGAAAA GGCCAAAGCT GCTCGGCCTC CGTGGGAACC TCCAAAGACG
    AAGCTCGATG AAGATTTAGA GAGTTCCAGT GAATCCGAGT GTGAGTCTGA TGAGGACAGC
    ACCTGTTCTA GCAGCTCAGA CTCTGAAGTT TTTGACGTTA TTGCAGAAAT CAAACGCAAA
    AAGGCCCACC CTGACCGACT TCATGATGAA CTTTGGTACA ACGATCCAGG CCAGATGAAT
    GATGGACCAC TCTGCAAATG CAGCGCAAAG GCAAGACGCA CAGGAATTAG GCACAGCATT
    TATCCTGGAG AAGAGGCCAT CAAGCCCTGT CGTCCTATGA CCAACAATGC TGGCAGACTT
    TTCCACTACC GGATCACAGT CTCCCCGCCT ACGAACTTTT TAACTGACAG GCCAACTGTT
    ATAGAATACG ATGATCACGA GTATATCTTT GAAGGATTTT CTATGTTTGC ACATGCCCCC
    CTGACCAATA TTCCACTGTG TAAAGTAATT AGATTCAACA TAGACTACAC GATTCATTTC
    ATTGAAGAGA TGATGCCGGA GAATTTTTGT GTGAAAGGGC TTGAACTCTT TTCACTGTTC
    CTATTCAGAG ATATTTTGGA ATTATATGAC TGGAATCTTA AAGGTCCTTT GTTTGAAGAC
    AGCCCTCCCT GCTGCCCAAG ATTTCATTTC ATGCCACGTT TTGTAAGATT TCTTCCAGAT
    GGAGGAAAGG AAGTGCTGTC CATGCACCAG ATTCTCCTGT ACTTGTTAAG GTGCAGCAAA
    GCCCTGGTGC CTGAGGAGGA GATTGCCAAT ATGCTTCAGT GGGAGGAGCT GGAGTGGCAG
    AAATATGCAG AAGAATGCAA AGGCATGATT GTTACCAACC CTGGGACGAA ACCAAGCTCT
    GTCCGTATCG ATCAACTGGA TCGTGAACAG TTCAACCCCG ATGTGATTAC TTTTCCGATT
    ATCGTCCACT TTGGGATACG CCCTGCACAG TACCAAAAAC TGTGGAAGAG TTATGTGAAA
    CTTCGCCACC TCCTAGCAAA TAGTCCCAAA GTCAAACAAA CTGACAAACA GAAGCTGGCA
    CAGAGGGAGG AAGCCCTCCA AAAAATACGG CAGAAGAATA CAATGAGACG AGAAGTAACG
    GTGGAGCTAA GTAGCCAAGG ATTCTGGAAA ACTGGCATCC GTTCTGATGT CTGTCAGCAT
    GCAATGATGC TACCTGTTCT GACCCATCAT ATCCGCTACC ACCAATGCCT AATGCATTTG
    GACAAGTTGA TAGGATATAC TTTCCAAGAT CGTTGTCTGT TGCAGCTGGC CATGACTCAT
    CCAAGTCATC ATTTAAATTT TGGAATGAAT CCTGATCATG CCAGGAATTC ATTATCTAAC
    TGTGGAATTC GGCAGCCCAA ATACGGAGAC AGAAAAGTTC ATCACATGCA CATGCGGAAG
    AAAGGGATTA ACACCTTGAT AAATATCATG TCACGCCTTG GCCAAGATGA CCCAACTCCC
    TCGAGGATTA ACCACAATGA ACGGTTGGAA TTCCTGGGTG ATGCTGTTGT TGAATTTCTG
    ACCAGCGTCC ATTTGTACTA TTTGTTTCCT AGTCTGGAAG AAGGAGGATT AGCAACCTAT
    CGGACTGCCA TTGTTCAGAA TCAGCACCTT GCCATGCTAG CAAAGAAACT TGAACTGGAT
    CGATTTATGC TGTATGCTCA CGGGCCTGAC CTTTGTAGAG AATCGGACCT TCGACATGCA
    ATGGCCAATT GTTTTGAAGC GTTAATAGGA GCTGTTTACT TGGAGGGAAG CCTGGAGGAA
    GCCAAGCAGT TATTTGGACG CTTGCTCTTT AATGATCCGG ACCTGCGCGA AGTCTGGCTC
    AATTATCCTC TCCACCCACT CCAACTACAA GAGCCAAATA CTGATCGACA ACTTATTGAA
    ACTTCTCCAG TTCTACAAAA ACTTACTGAG TTTGAAGAAG CAATTGGAGT AATTTTTACT
    CATGTTCGAC TTCTGGCAAG GGCATTCACA TTGAGAACTG TGGGATTTAA CCATCTGACC
    CTAGGCCACA ATCAGAGAAT GGAATTCCTA GGTGACTCCA TAATGCAACT GGTAGCCACA
    GAGTACTTAT TCATTCATTT CCCAGATCAT CATGAAGGAC ACTTAACTTT GTTGCGAAGC
    TCTTTGGTGA ATAATAGAAC TCAGGCCAAG GTAGCGGAGG AGCTGGGCAT GCAGGAGTAC
    GCCATAACCA ACGACAAGAC CAAGAGGCCT GTGGCGCTTC GCACCAAGAC CTTGGCGGAC
    CTTTTGGAAT CATTTATTGC AGCGCTGTAC ATTGATAAGG ATTTGGAATA TGTTCATACT
    TTCATGAATG TCTGCTTCTT TCCACGATTG AAAGAGTTCA TTTTGAATCA GGATTGGAAT
    GACCCCAAAT CCCAGCTTCA GCAGTGTTGC TTGACACTTA GGACAGAAGG AAAAGAGCCA
    GACATTCCTC TGTACAAGAC TCTGCAGACA GTGGGCCCAT CCCATGCCCG AACCTACACT
    GTGGCTGTTT ATTTCAAGGG AGAAAGAATA GGCTGTGGGA AAGGACCAAG TATTCAGCAA
    GCGGAAATGG GAGCAGCAAT GGATGCGCTT GAAAAATATA ATTTTCCCCA GATGGCCCAT
    CAGAAGCGGT TCATCGAACG GAAGTACAGA CAAGAGTTAA AAGAAATGAG GTGGGAAAGA
    GAGCATCAAG AGAGAGAGCC AGATGAGACT GAAGACATCA AGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95847
    Clone ID Related Accession (Same CDS sequence) XM_017009399.1 , XM_017009399.2
    Accession Version XM_017009399.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGGAGTTA CGGTTTATCG GTTGTTCCTG AACCTGCTGG ATGCACACCA
    GAATTACCTG GGGAGATTAT TAAAAATACA GATTCTTGGG CCCCACCCCT GGAGATTGTG
    AATCATCGCT CCCCAAGTAG GGAGAAGAAG AGAGCTCGTT GGGAGGAAGA AAAAGACCGT
    TGGAGTGACA ACCAGAGTTC TGGCAAAGAC AAGAACTATA CCTCAATCAA GGAAAAAGAG
    CCCGAGGAGA CCATGCCTGA CAAGAATGAG GAGGAAGAAG AAGAACTTCT TAAGCCTGTG
    TGGATTCGAT GCACTCATTC AGAAAACTAC TACTCCAGTG ACCCCATGGA TCAGGTGGGA
    GATTCTACAG TGGTTGGAAC GAGTAGGCTT CGTGACTTAT ATGACAAATT TGAGGAGGAG
    TTGGGGAGCA GGCAAGAAAA GGCCAAAGCT GCTCGGCCTC CGTGGGAACC TCCAAAGACG
    AAGCTCGATG AAGATTTAGA GAGTTCCAGT GAATCCGAGT GTGAGTCTGA TGAGGACAGC
    ACCTGTTCTA GCAGCTCAGA CTCTGAAGTT TTTGACGTTA TTGCAGAAAT CAAACGCAAA
    AAGGCCCACC CTGACCGACT TCATGATGAA CTTTGGTACA ACGATCCAGG CCAGATGAAT
    GATGGACCAC TCTGCAAATG CAGCGCAAAG GCAAGACGCA CAGGAATTAG GCACAGCATT
    TATCCTGGAG AAGAGGCCAT CAAGCCCTGT CGTCCTATGA CCAACAATGC TGGCAGACTT
    TTCCACTACC GGATCACAGT CTCCCCGCCT ACGAACTTTT TAACTGACAG GCCAACTGTT
    ATAGAATACG ATGATCACGA GTATATCTTT GAAGGATTTT CTATGTTTGC ACATGCCCCC
    CTGACCAATA TTCCACTGTG TAAAGTAATT AGATTCAACA TAGACTACAC GATTCATTTC
    ATTGAAGAGA TGATGCCGGA GAATTTTTGT GTGAAAGGGC TTGAACTCTT TTCACTGTTC
    CTATTCAGAG ATATTTTGGA ATTATATGAC TGGAATCTTA AAGGTCCTTT GTTTGAAGAC
    AGCCCTCCCT GCTGCCCAAG ATTTCATTTC ATGCCACGTT TTGTAAGATT TCTTCCAGAT
    GGAGGAAAGG AAGTGCTGTC CATGCACCAG ATTCTCCTGT ACTTGTTAAG GTGCAGCAAA
    GCCCTGGTGC CTGAGGAGGA GATTGCCAAT ATGCTTCAGT GGGAGGAGCT GGAGTGGCAG
    AAATATGCAG AAGAATGCAA AGGCATGATT GTTACCAACC CTGGGACGAA ACCAAGCTCT
    GTCCGTATCG ATCAACTGGA TCGTGAACAG TTCAACCCCG ATGTGATTAC TTTTCCGATT
    ATCGTCCACT TTGGGATACG CCCTGCACAG TACCAAAAAC TGTGGAAGAG TTATGTGAAA
    CTTCGCCACC TCCTAGCAAA TAGTCCCAAA GTCAAACAAA CTGACAAACA GAAGCTGGCA
    CAGAGGGAGG AAGCCCTCCA AAAAATACGG CAGAAGAATA CAATGAGACG AGAAGTAACG
    GTGGAGCTAA GTAGCCAAGG ATTCTGGAAA ACTGGCATCC GTTCTGATGT CTGTCAGCAT
    GCAATGATGC TACCTGTTCT GACCCATCAT ATCCGCTACC ACCAATGCCT AATGCATTTG
    GACAAGTTGA TAGGATATAC TTTCCAAGAT CGTTGTCTGT TGCAGCTGGC CATGACTCAT
    CCAAGTCATC ATTTAAATTT TGGAATGAAT CCTGATCATG CCAGGAATTC ATTATCTAAC
    TGTGGAATTC GGCAGCCCAA ATACGGAGAC AGAAAAGTTC ATCACATGCA CATGCGGAAG
    AAAGGGATTA ACACCTTGAT AAATATCATG TCACGCCTTG GCCAAGATGA CCCAACTCCC
    TCGAGGATTA ACCACAATGA ACGGTTGGAA TTCCTGGGTG ATGCTGTTGT TGAATTTCTG
    ACCAGCGTCC ATTTGTACTA TTTGTTTCCT AGTCTGGAAG AAGGAGGATT AGCAACCTAT
    CGGACTGCCA TTGTTCAGAA TCAGCACCTT GCCATGCTAG CAAAGAAACT TGAACTGGAT
    CGATTTATGC TGTATGCTCA CGGGCCTGAC CTTTGTAGAG AATCGGACCT TCGACATGCA
    ATGGCCAATT GTTTTGAAGC GTTAATAGGA GCTGTTTACT TGGAGGGAAG CCTGGAGGAA
    GCCAAGCAGT TATTTGGACG CTTGCTCTTT AATGATCCGG ACCTGCGCGA AGTCTGGCTC
    AATTATCCTC TCCACCCACT CCAACTACAA GAGCCAAATA CTGATCGACA ACTTATTGAA
    ACTTCTCCAG TTCTACAAAA ACTTACTGAG TTTGAAGAAG CAATTGGAGT AATTTTTACT
    CATGTTCGAC TTCTGGCAAG GGCATTCACA TTGAGAACTG TGGGATTTAA CCATCTGACC
    CTAGGCCACA ATCAGAGAAT GGAATTCCTA GGTGACTCCA TAATGCAACT GGTAGCCACA
    GAGTACTTAT TCATTCATTT CCCAGATCAT CATGAAGGAC ACTTAACTTT GTTGCGAAGC
    TCTTTGGTGA ATAATAGAAC TCAGGCCAAG GTAGCGGAGG AGCTGGGCAT GCAGGAGTAC
    GCCATAACCA ACGACAAGAC CAAGAGGCCT GTGGCGCTTC GCACCAAGAC CTTGGCGGAC
    CTTTTGGAAT CATTTATTGC AGCGCTGTAC ATTGATAAGG ATTTGGAATA TGTTCATACT
    TTCATGAATG TCTGCTTCTT TCCACGATTG AAAGAGTTCA TTTTGAATCA GGATTGGAAT
    GACCCCAAAT CCCAGCTTCA GCAGTGTTGC TTGACACTTA GGACAGAAGG AAAAGAGCCA
    GACATTCCTC TGTACAAGAC TCTGCAGACA GTGGGCCCAT CCCATGCCCG AACCTACACT
    GTGGCTGTTT ATTTCAAGGG AGAAAGAATA GGCTGTGGGA AAGGACCAAG TATTCAGCAA
    GCGGAAATGG GAGCAGCAAT GGATGCGCTT GAAAAATATA ATTTTCCCCA GATGGCCCAT
    CAGAAGCGGT TCATCGAACG GAAGTACAGA CAAGAGTTAA AAGAAATGAG GTGGGAAAGA
    GAGCATCAAG AGAGAGAGCC AGATGAGACT GAAGACATCA AGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864888.1
    CDS403..4410
    Translation

    Target ORF information:

    RefSeq Version XM_017009399.2
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009399.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGGAGTTA CGGTTTATCG GTTGTTCCTG AACCTGCTGG ATGCACACCA
    GAATTACCTG GGGAGATTAT TAAAAATACA GATTCTTGGG CCCCACCCCT GGAGATTGTG
    AATCATCGCT CCCCAAGTAG GGAGAAGAAG AGAGCTCGTT GGGAGGAAGA AAAAGACCGT
    TGGAGTGACA ACCAGAGTTC TGGCAAAGAC AAGAACTATA CCTCAATCAA GGAAAAAGAG
    CCCGAGGAGA CCATGCCTGA CAAGAATGAG GAGGAAGAAG AAGAACTTCT TAAGCCTGTG
    TGGATTCGAT GCACTCATTC AGAAAACTAC TACTCCAGTG ACCCCATGGA TCAGGTGGGA
    GATTCTACAG TGGTTGGAAC GAGTAGGCTT CGTGACTTAT ATGACAAATT TGAGGAGGAG
    TTGGGGAGCA GGCAAGAAAA GGCCAAAGCT GCTCGGCCTC CGTGGGAACC TCCAAAGACG
    AAGCTCGATG AAGATTTAGA GAGTTCCAGT GAATCCGAGT GTGAGTCTGA TGAGGACAGC
    ACCTGTTCTA GCAGCTCAGA CTCTGAAGTT TTTGACGTTA TTGCAGAAAT CAAACGCAAA
    AAGGCCCACC CTGACCGACT TCATGATGAA CTTTGGTACA ACGATCCAGG CCAGATGAAT
    GATGGACCAC TCTGCAAATG CAGCGCAAAG GCAAGACGCA CAGGAATTAG GCACAGCATT
    TATCCTGGAG AAGAGGCCAT CAAGCCCTGT CGTCCTATGA CCAACAATGC TGGCAGACTT
    TTCCACTACC GGATCACAGT CTCCCCGCCT ACGAACTTTT TAACTGACAG GCCAACTGTT
    ATAGAATACG ATGATCACGA GTATATCTTT GAAGGATTTT CTATGTTTGC ACATGCCCCC
    CTGACCAATA TTCCACTGTG TAAAGTAATT AGATTCAACA TAGACTACAC GATTCATTTC
    ATTGAAGAGA TGATGCCGGA GAATTTTTGT GTGAAAGGGC TTGAACTCTT TTCACTGTTC
    CTATTCAGAG ATATTTTGGA ATTATATGAC TGGAATCTTA AAGGTCCTTT GTTTGAAGAC
    AGCCCTCCCT GCTGCCCAAG ATTTCATTTC ATGCCACGTT TTGTAAGATT TCTTCCAGAT
    GGAGGAAAGG AAGTGCTGTC CATGCACCAG ATTCTCCTGT ACTTGTTAAG GTGCAGCAAA
    GCCCTGGTGC CTGAGGAGGA GATTGCCAAT ATGCTTCAGT GGGAGGAGCT GGAGTGGCAG
    AAATATGCAG AAGAATGCAA AGGCATGATT GTTACCAACC CTGGGACGAA ACCAAGCTCT
    GTCCGTATCG ATCAACTGGA TCGTGAACAG TTCAACCCCG ATGTGATTAC TTTTCCGATT
    ATCGTCCACT TTGGGATACG CCCTGCACAG TACCAAAAAC TGTGGAAGAG TTATGTGAAA
    CTTCGCCACC TCCTAGCAAA TAGTCCCAAA GTCAAACAAA CTGACAAACA GAAGCTGGCA
    CAGAGGGAGG AAGCCCTCCA AAAAATACGG CAGAAGAATA CAATGAGACG AGAAGTAACG
    GTGGAGCTAA GTAGCCAAGG ATTCTGGAAA ACTGGCATCC GTTCTGATGT CTGTCAGCAT
    GCAATGATGC TACCTGTTCT GACCCATCAT ATCCGCTACC ACCAATGCCT AATGCATTTG
    GACAAGTTGA TAGGATATAC TTTCCAAGAT CGTTGTCTGT TGCAGCTGGC CATGACTCAT
    CCAAGTCATC ATTTAAATTT TGGAATGAAT CCTGATCATG CCAGGAATTC ATTATCTAAC
    TGTGGAATTC GGCAGCCCAA ATACGGAGAC AGAAAAGTTC ATCACATGCA CATGCGGAAG
    AAAGGGATTA ACACCTTGAT AAATATCATG TCACGCCTTG GCCAAGATGA CCCAACTCCC
    TCGAGGATTA ACCACAATGA ACGGTTGGAA TTCCTGGGTG ATGCTGTTGT TGAATTTCTG
    ACCAGCGTCC ATTTGTACTA TTTGTTTCCT AGTCTGGAAG AAGGAGGATT AGCAACCTAT
    CGGACTGCCA TTGTTCAGAA TCAGCACCTT GCCATGCTAG CAAAGAAACT TGAACTGGAT
    CGATTTATGC TGTATGCTCA CGGGCCTGAC CTTTGTAGAG AATCGGACCT TCGACATGCA
    ATGGCCAATT GTTTTGAAGC GTTAATAGGA GCTGTTTACT TGGAGGGAAG CCTGGAGGAA
    GCCAAGCAGT TATTTGGACG CTTGCTCTTT AATGATCCGG ACCTGCGCGA AGTCTGGCTC
    AATTATCCTC TCCACCCACT CCAACTACAA GAGCCAAATA CTGATCGACA ACTTATTGAA
    ACTTCTCCAG TTCTACAAAA ACTTACTGAG TTTGAAGAAG CAATTGGAGT AATTTTTACT
    CATGTTCGAC TTCTGGCAAG GGCATTCACA TTGAGAACTG TGGGATTTAA CCATCTGACC
    CTAGGCCACA ATCAGAGAAT GGAATTCCTA GGTGACTCCA TAATGCAACT GGTAGCCACA
    GAGTACTTAT TCATTCATTT CCCAGATCAT CATGAAGGAC ACTTAACTTT GTTGCGAAGC
    TCTTTGGTGA ATAATAGAAC TCAGGCCAAG GTAGCGGAGG AGCTGGGCAT GCAGGAGTAC
    GCCATAACCA ACGACAAGAC CAAGAGGCCT GTGGCGCTTC GCACCAAGAC CTTGGCGGAC
    CTTTTGGAAT CATTTATTGC AGCGCTGTAC ATTGATAAGG ATTTGGAATA TGTTCATACT
    TTCATGAATG TCTGCTTCTT TCCACGATTG AAAGAGTTCA TTTTGAATCA GGATTGGAAT
    GACCCCAAAT CCCAGCTTCA GCAGTGTTGC TTGACACTTA GGACAGAAGG AAAAGAGCCA
    GACATTCCTC TGTACAAGAC TCTGCAGACA GTGGGCCCAT CCCATGCCCG AACCTACACT
    GTGGCTGTTT ATTTCAAGGG AGAAAGAATA GGCTGTGGGA AAGGACCAAG TATTCAGCAA
    GCGGAAATGG GAGCAGCAAT GGATGCGCTT GAAAAATATA ATTTTCCCCA GATGGCCCAT
    CAGAAGCGGT TCATCGAACG GAAGTACAGA CAAGAGTTAA AAGAAATGAG GTGGGAAAGA
    GAGCATCAAG AGAGAGAGCC AGATGAGACT GAAGACATCA AGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95848
    Clone ID Related Accession (Same CDS sequence) XM_017009400.1 , XM_017009400.2
    Accession Version XM_017009400.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGTCG CTCCCCAAGT
    AGGGAGAAGA AGAGAGCTCG TTGGGAGGAA GAAAAAGACC GTTGGAGTGA CAACCAGAGT
    TCTGGCAAAG ACAAGAACTA TACCTCAATC AAGGAAAAAG AGCCCGAGGA GACCATGCCT
    GACAAGAATG AGGAGGAAGA AGAAGAACTT CTTAAGCCTG TGTGGATTCG ATGCACTCAT
    TCAGAAAACT ACTACTCCAG TGACCCCATG GATCAGGTGG GAGATTCTAC AGTGGTTGGA
    ACGAGTAGGC TTCGTGACTT ATATGACAAA TTTGAGGAGG AGTTGGGGAG CAGGCAAGAA
    AAGGCCAAAG CTGCTCGGCC TCCGTGGGAA CCTCCAAAGA CGAAGCTCGA TGAAGATTTA
    GAGAGTTCCA GTGAATCCGA GTGTGAGTCT GATGAGGACA GCACCTGTTC TAGCAGCTCA
    GACTCTGAAG TTTTTGACGT TATTGCAGAA ATCAAACGCA AAAAGGCCCA CCCTGACCGA
    CTTCATGATG AACTTTGGTA CAACGATCCA GGCCAGATGA ATGATGGACC ACTCTGCAAA
    TGCAGCGCAA AGGCAAGACG CACAGGAATT AGGCACAGCA TTTATCCTGG AGAAGAGGCC
    ATCAAGCCCT GTCGTCCTAT GACCAACAAT GCTGGCAGAC TTTTCCACTA CCGGATCACA
    GTCTCCCCGC CTACGAACTT TTTAACTGAC AGGCCAACTG TTATAGAATA CGATGATCAC
    GAGTATATCT TTGAAGGATT TTCTATGTTT GCACATGCCC CCCTGACCAA TATTCCACTG
    TGTAAAGTAA TTAGATTCAA CATAGACTAC ACGATTCATT TCATTGAAGA GATGATGCCG
    GAGAATTTTT GTGTGAAAGG GCTTGAACTC TTTTCACTGT TCCTATTCAG AGATATTTTG
    GAATTATATG ACTGGAATCT TAAAGGTCCT TTGTTTGAAG ACAGCCCTCC CTGCTGCCCA
    AGATTTCATT TCATGCCACG TTTTGTAAGA TTTCTTCCAG ATGGAGGAAA GGAAGTGCTG
    TCCATGCACC AGATTCTCCT GTACTTGTTA AGGTGCAGCA AAGCCCTGGT GCCTGAGGAG
    GAGATTGCCA ATATGCTTCA GTGGGAGGAG CTGGAGTGGC AGAAATATGC AGAAGAATGC
    AAAGGCATGA TTGTTACCAA CCCTGGGACG AAACCAAGCT CTGTCCGTAT CGATCAACTG
    GATCGTGAAC AGTTCAACCC CGATGTGATT ACTTTTCCGA TTATCGTCCA CTTTGGGATA
    CGCCCTGCAC AGTACCAAAA ACTGTGGAAG AGTTATGTGA AACTTCGCCA CCTCCTAGCA
    AATAGTCCCA AAGTCAAACA AACTGACAAA CAGAAGCTGG CACAGAGGGA GGAAGCCCTC
    CAAAAAATAC GGCAGAAGAA TACAATGAGA CGAGAAGTAA CGGTGGAGCT AAGTAGCCAA
    GGATTCTGGA AAACTGGCAT CCGTTCTGAT GTCTGTCAGC ATGCAATGAT GCTACCTGTT
    CTGACCCATC ATATCCGCTA CCACCAATGC CTAATGCATT TGGACAAGTT GATAGGATAT
    ACTTTCCAAG ATCGTTGTCT GTTGCAGCTG GCCATGACTC ATCCAAGTCA TCATTTAAAT
    TTTGGAATGA ATCCTGATCA TGCCAGGAAT TCATTATCTA ACTGTGGAAT TCGGCAGCCC
    AAATACGGAG ACAGAAAAGT TCATCACATG CACATGCGGA AGAAAGGGAT TAACACCTTG
    ATAAATATCA TGTCACGCCT TGGCCAAGAT GACCCAACTC CCTCGAGGAT TAACCACAAT
    GAACGGTTGG AATTCCTGGG TGATGCTGTT GTTGAATTTC TGACCAGCGT CCATTTGTAC
    TATTTGTTTC CTAGTCTGGA AGAAGGAGGA TTAGCAACCT ATCGGACTGC CATTGTTCAG
    AATCAGCACC TTGCCATGCT AGCAAAGAAA CTTGAACTGG ATCGATTTAT GCTGTATGCT
    CACGGGCCTG ACCTTTGTAG AGAATCGGAC CTTCGACATG CAATGGCCAA TTGTTTTGAA
    GCGTTAATAG GAGCTGTTTA CTTGGAGGGA AGCCTGGAGG AAGCCAAGCA GTTATTTGGA
    CGCTTGCTCT TTAATGATCC GGACCTGCGC GAAGTCTGGC TCAATTATCC TCTCCACCCA
    CTCCAACTAC AAGAGCCAAA TACTGATCGA CAACTTATTG AAACTTCTCC AGTTCTACAA
    AAACTTACTG AGTTTGAAGA AGCAATTGGA GTAATTTTTA CTCATGTTCG ACTTCTGGCA
    AGGGCATTCA CATTGAGAAC TGTGGGATTT AACCATCTGA CCCTAGGCCA CAATCAGAGA
    ATGGAATTCC TAGGTGACTC CATAATGCAA CTGGTAGCCA CAGAGTACTT ATTCATTCAT
    TTCCCAGATC ATCATGAAGG ACACTTAACT TTGTTGCGAA GCTCTTTGGT GAATAATAGA
    ACTCAGGCCA AGGTAGCGGA GGAGCTGGGC ATGCAGGAGT ACGCCATAAC CAACGACAAG
    ACCAAGAGGC CTGTGGCGCT TCGCACCAAG ACCTTGGCGG ACCTTTTGGA ATCATTTATT
    GCAGCGCTGT ACATTGATAA GGATTTGGAA TATGTTCATA CTTTCATGAA TGTCTGCTTC
    TTTCCACGAT TGAAAGAGTT CATTTTGAAT CAGGATTGGA ATGACCCCAA ATCCCAGCTT
    CAGCAGTGTT GCTTGACACT TAGGACAGAA GGAAAAGAGC CAGACATTCC TCTGTACAAG
    ACTCTGCAGA CAGTGGGCCC ATCCCATGCC CGAACCTACA CTGTGGCTGT TTATTTCAAG
    GGAGAAAGAA TAGGCTGTGG GAAAGGACCA AGTATTCAGC AAGCGGAAAT GGGAGCAGCA
    ATGGATGCGC TTGAAAAATA TAATTTTCCC CAGATGGCCC ATCAGAAGCG GTTCATCGAA
    CGGAAGTACA GACAAGAGTT AAAAGAAATG AGGTGGGAAA GAGAGCATCA AGAGAGAGAG
    CCAGATGAGA CTGAAGACAT CAAGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864889.1
    CDS436..4425
    Translation

    Target ORF information:

    RefSeq Version XM_017009400.1
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009400.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGTCG CTCCCCAAGT
    AGGGAGAAGA AGAGAGCTCG TTGGGAGGAA GAAAAAGACC GTTGGAGTGA CAACCAGAGT
    TCTGGCAAAG ACAAGAACTA TACCTCAATC AAGGAAAAAG AGCCCGAGGA GACCATGCCT
    GACAAGAATG AGGAGGAAGA AGAAGAACTT CTTAAGCCTG TGTGGATTCG ATGCACTCAT
    TCAGAAAACT ACTACTCCAG TGACCCCATG GATCAGGTGG GAGATTCTAC AGTGGTTGGA
    ACGAGTAGGC TTCGTGACTT ATATGACAAA TTTGAGGAGG AGTTGGGGAG CAGGCAAGAA
    AAGGCCAAAG CTGCTCGGCC TCCGTGGGAA CCTCCAAAGA CGAAGCTCGA TGAAGATTTA
    GAGAGTTCCA GTGAATCCGA GTGTGAGTCT GATGAGGACA GCACCTGTTC TAGCAGCTCA
    GACTCTGAAG TTTTTGACGT TATTGCAGAA ATCAAACGCA AAAAGGCCCA CCCTGACCGA
    CTTCATGATG AACTTTGGTA CAACGATCCA GGCCAGATGA ATGATGGACC ACTCTGCAAA
    TGCAGCGCAA AGGCAAGACG CACAGGAATT AGGCACAGCA TTTATCCTGG AGAAGAGGCC
    ATCAAGCCCT GTCGTCCTAT GACCAACAAT GCTGGCAGAC TTTTCCACTA CCGGATCACA
    GTCTCCCCGC CTACGAACTT TTTAACTGAC AGGCCAACTG TTATAGAATA CGATGATCAC
    GAGTATATCT TTGAAGGATT TTCTATGTTT GCACATGCCC CCCTGACCAA TATTCCACTG
    TGTAAAGTAA TTAGATTCAA CATAGACTAC ACGATTCATT TCATTGAAGA GATGATGCCG
    GAGAATTTTT GTGTGAAAGG GCTTGAACTC TTTTCACTGT TCCTATTCAG AGATATTTTG
    GAATTATATG ACTGGAATCT TAAAGGTCCT TTGTTTGAAG ACAGCCCTCC CTGCTGCCCA
    AGATTTCATT TCATGCCACG TTTTGTAAGA TTTCTTCCAG ATGGAGGAAA GGAAGTGCTG
    TCCATGCACC AGATTCTCCT GTACTTGTTA AGGTGCAGCA AAGCCCTGGT GCCTGAGGAG
    GAGATTGCCA ATATGCTTCA GTGGGAGGAG CTGGAGTGGC AGAAATATGC AGAAGAATGC
    AAAGGCATGA TTGTTACCAA CCCTGGGACG AAACCAAGCT CTGTCCGTAT CGATCAACTG
    GATCGTGAAC AGTTCAACCC CGATGTGATT ACTTTTCCGA TTATCGTCCA CTTTGGGATA
    CGCCCTGCAC AGTACCAAAA ACTGTGGAAG AGTTATGTGA AACTTCGCCA CCTCCTAGCA
    AATAGTCCCA AAGTCAAACA AACTGACAAA CAGAAGCTGG CACAGAGGGA GGAAGCCCTC
    CAAAAAATAC GGCAGAAGAA TACAATGAGA CGAGAAGTAA CGGTGGAGCT AAGTAGCCAA
    GGATTCTGGA AAACTGGCAT CCGTTCTGAT GTCTGTCAGC ATGCAATGAT GCTACCTGTT
    CTGACCCATC ATATCCGCTA CCACCAATGC CTAATGCATT TGGACAAGTT GATAGGATAT
    ACTTTCCAAG ATCGTTGTCT GTTGCAGCTG GCCATGACTC ATCCAAGTCA TCATTTAAAT
    TTTGGAATGA ATCCTGATCA TGCCAGGAAT TCATTATCTA ACTGTGGAAT TCGGCAGCCC
    AAATACGGAG ACAGAAAAGT TCATCACATG CACATGCGGA AGAAAGGGAT TAACACCTTG
    ATAAATATCA TGTCACGCCT TGGCCAAGAT GACCCAACTC CCTCGAGGAT TAACCACAAT
    GAACGGTTGG AATTCCTGGG TGATGCTGTT GTTGAATTTC TGACCAGCGT CCATTTGTAC
    TATTTGTTTC CTAGTCTGGA AGAAGGAGGA TTAGCAACCT ATCGGACTGC CATTGTTCAG
    AATCAGCACC TTGCCATGCT AGCAAAGAAA CTTGAACTGG ATCGATTTAT GCTGTATGCT
    CACGGGCCTG ACCTTTGTAG AGAATCGGAC CTTCGACATG CAATGGCCAA TTGTTTTGAA
    GCGTTAATAG GAGCTGTTTA CTTGGAGGGA AGCCTGGAGG AAGCCAAGCA GTTATTTGGA
    CGCTTGCTCT TTAATGATCC GGACCTGCGC GAAGTCTGGC TCAATTATCC TCTCCACCCA
    CTCCAACTAC AAGAGCCAAA TACTGATCGA CAACTTATTG AAACTTCTCC AGTTCTACAA
    AAACTTACTG AGTTTGAAGA AGCAATTGGA GTAATTTTTA CTCATGTTCG ACTTCTGGCA
    AGGGCATTCA CATTGAGAAC TGTGGGATTT AACCATCTGA CCCTAGGCCA CAATCAGAGA
    ATGGAATTCC TAGGTGACTC CATAATGCAA CTGGTAGCCA CAGAGTACTT ATTCATTCAT
    TTCCCAGATC ATCATGAAGG ACACTTAACT TTGTTGCGAA GCTCTTTGGT GAATAATAGA
    ACTCAGGCCA AGGTAGCGGA GGAGCTGGGC ATGCAGGAGT ACGCCATAAC CAACGACAAG
    ACCAAGAGGC CTGTGGCGCT TCGCACCAAG ACCTTGGCGG ACCTTTTGGA ATCATTTATT
    GCAGCGCTGT ACATTGATAA GGATTTGGAA TATGTTCATA CTTTCATGAA TGTCTGCTTC
    TTTCCACGAT TGAAAGAGTT CATTTTGAAT CAGGATTGGA ATGACCCCAA ATCCCAGCTT
    CAGCAGTGTT GCTTGACACT TAGGACAGAA GGAAAAGAGC CAGACATTCC TCTGTACAAG
    ACTCTGCAGA CAGTGGGCCC ATCCCATGCC CGAACCTACA CTGTGGCTGT TTATTTCAAG
    GGAGAAAGAA TAGGCTGTGG GAAAGGACCA AGTATTCAGC AAGCGGAAAT GGGAGCAGCA
    ATGGATGCGC TTGAAAAATA TAATTTTCCC CAGATGGCCC ATCAGAAGCG GTTCATCGAA
    CGGAAGTACA GACAAGAGTT AAAAGAAATG AGGTGGGAAA GAGAGCATCA AGAGAGAGAG
    CCAGATGAGA CTGAAGACAT CAAGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95848
    Clone ID Related Accession (Same CDS sequence) XM_017009400.1 , XM_017009400.2
    Accession Version XM_017009400.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009400.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGTCG CTCCCCAAGT
    AGGGAGAAGA AGAGAGCTCG TTGGGAGGAA GAAAAAGACC GTTGGAGTGA CAACCAGAGT
    TCTGGCAAAG ACAAGAACTA TACCTCAATC AAGGAAAAAG AGCCCGAGGA GACCATGCCT
    GACAAGAATG AGGAGGAAGA AGAAGAACTT CTTAAGCCTG TGTGGATTCG ATGCACTCAT
    TCAGAAAACT ACTACTCCAG TGACCCCATG GATCAGGTGG GAGATTCTAC AGTGGTTGGA
    ACGAGTAGGC TTCGTGACTT ATATGACAAA TTTGAGGAGG AGTTGGGGAG CAGGCAAGAA
    AAGGCCAAAG CTGCTCGGCC TCCGTGGGAA CCTCCAAAGA CGAAGCTCGA TGAAGATTTA
    GAGAGTTCCA GTGAATCCGA GTGTGAGTCT GATGAGGACA GCACCTGTTC TAGCAGCTCA
    GACTCTGAAG TTTTTGACGT TATTGCAGAA ATCAAACGCA AAAAGGCCCA CCCTGACCGA
    CTTCATGATG AACTTTGGTA CAACGATCCA GGCCAGATGA ATGATGGACC ACTCTGCAAA
    TGCAGCGCAA AGGCAAGACG CACAGGAATT AGGCACAGCA TTTATCCTGG AGAAGAGGCC
    ATCAAGCCCT GTCGTCCTAT GACCAACAAT GCTGGCAGAC TTTTCCACTA CCGGATCACA
    GTCTCCCCGC CTACGAACTT TTTAACTGAC AGGCCAACTG TTATAGAATA CGATGATCAC
    GAGTATATCT TTGAAGGATT TTCTATGTTT GCACATGCCC CCCTGACCAA TATTCCACTG
    TGTAAAGTAA TTAGATTCAA CATAGACTAC ACGATTCATT TCATTGAAGA GATGATGCCG
    GAGAATTTTT GTGTGAAAGG GCTTGAACTC TTTTCACTGT TCCTATTCAG AGATATTTTG
    GAATTATATG ACTGGAATCT TAAAGGTCCT TTGTTTGAAG ACAGCCCTCC CTGCTGCCCA
    AGATTTCATT TCATGCCACG TTTTGTAAGA TTTCTTCCAG ATGGAGGAAA GGAAGTGCTG
    TCCATGCACC AGATTCTCCT GTACTTGTTA AGGTGCAGCA AAGCCCTGGT GCCTGAGGAG
    GAGATTGCCA ATATGCTTCA GTGGGAGGAG CTGGAGTGGC AGAAATATGC AGAAGAATGC
    AAAGGCATGA TTGTTACCAA CCCTGGGACG AAACCAAGCT CTGTCCGTAT CGATCAACTG
    GATCGTGAAC AGTTCAACCC CGATGTGATT ACTTTTCCGA TTATCGTCCA CTTTGGGATA
    CGCCCTGCAC AGTACCAAAA ACTGTGGAAG AGTTATGTGA AACTTCGCCA CCTCCTAGCA
    AATAGTCCCA AAGTCAAACA AACTGACAAA CAGAAGCTGG CACAGAGGGA GGAAGCCCTC
    CAAAAAATAC GGCAGAAGAA TACAATGAGA CGAGAAGTAA CGGTGGAGCT AAGTAGCCAA
    GGATTCTGGA AAACTGGCAT CCGTTCTGAT GTCTGTCAGC ATGCAATGAT GCTACCTGTT
    CTGACCCATC ATATCCGCTA CCACCAATGC CTAATGCATT TGGACAAGTT GATAGGATAT
    ACTTTCCAAG ATCGTTGTCT GTTGCAGCTG GCCATGACTC ATCCAAGTCA TCATTTAAAT
    TTTGGAATGA ATCCTGATCA TGCCAGGAAT TCATTATCTA ACTGTGGAAT TCGGCAGCCC
    AAATACGGAG ACAGAAAAGT TCATCACATG CACATGCGGA AGAAAGGGAT TAACACCTTG
    ATAAATATCA TGTCACGCCT TGGCCAAGAT GACCCAACTC CCTCGAGGAT TAACCACAAT
    GAACGGTTGG AATTCCTGGG TGATGCTGTT GTTGAATTTC TGACCAGCGT CCATTTGTAC
    TATTTGTTTC CTAGTCTGGA AGAAGGAGGA TTAGCAACCT ATCGGACTGC CATTGTTCAG
    AATCAGCACC TTGCCATGCT AGCAAAGAAA CTTGAACTGG ATCGATTTAT GCTGTATGCT
    CACGGGCCTG ACCTTTGTAG AGAATCGGAC CTTCGACATG CAATGGCCAA TTGTTTTGAA
    GCGTTAATAG GAGCTGTTTA CTTGGAGGGA AGCCTGGAGG AAGCCAAGCA GTTATTTGGA
    CGCTTGCTCT TTAATGATCC GGACCTGCGC GAAGTCTGGC TCAATTATCC TCTCCACCCA
    CTCCAACTAC AAGAGCCAAA TACTGATCGA CAACTTATTG AAACTTCTCC AGTTCTACAA
    AAACTTACTG AGTTTGAAGA AGCAATTGGA GTAATTTTTA CTCATGTTCG ACTTCTGGCA
    AGGGCATTCA CATTGAGAAC TGTGGGATTT AACCATCTGA CCCTAGGCCA CAATCAGAGA
    ATGGAATTCC TAGGTGACTC CATAATGCAA CTGGTAGCCA CAGAGTACTT ATTCATTCAT
    TTCCCAGATC ATCATGAAGG ACACTTAACT TTGTTGCGAA GCTCTTTGGT GAATAATAGA
    ACTCAGGCCA AGGTAGCGGA GGAGCTGGGC ATGCAGGAGT ACGCCATAAC CAACGACAAG
    ACCAAGAGGC CTGTGGCGCT TCGCACCAAG ACCTTGGCGG ACCTTTTGGA ATCATTTATT
    GCAGCGCTGT ACATTGATAA GGATTTGGAA TATGTTCATA CTTTCATGAA TGTCTGCTTC
    TTTCCACGAT TGAAAGAGTT CATTTTGAAT CAGGATTGGA ATGACCCCAA ATCCCAGCTT
    CAGCAGTGTT GCTTGACACT TAGGACAGAA GGAAAAGAGC CAGACATTCC TCTGTACAAG
    ACTCTGCAGA CAGTGGGCCC ATCCCATGCC CGAACCTACA CTGTGGCTGT TTATTTCAAG
    GGAGAAAGAA TAGGCTGTGG GAAAGGACCA AGTATTCAGC AAGCGGAAAT GGGAGCAGCA
    ATGGATGCGC TTGAAAAATA TAATTTTCCC CAGATGGCCC ATCAGAAGCG GTTCATCGAA
    CGGAAGTACA GACAAGAGTT AAAAGAAATG AGGTGGGAAA GAGAGCATCA AGAGAGAGAG
    CCAGATGAGA CTGAAGACAT CAAGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864889.1
    CDS403..4392
    Translation

    Target ORF information:

    RefSeq Version XM_017009400.2
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009400.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGTCG CTCCCCAAGT
    AGGGAGAAGA AGAGAGCTCG TTGGGAGGAA GAAAAAGACC GTTGGAGTGA CAACCAGAGT
    TCTGGCAAAG ACAAGAACTA TACCTCAATC AAGGAAAAAG AGCCCGAGGA GACCATGCCT
    GACAAGAATG AGGAGGAAGA AGAAGAACTT CTTAAGCCTG TGTGGATTCG ATGCACTCAT
    TCAGAAAACT ACTACTCCAG TGACCCCATG GATCAGGTGG GAGATTCTAC AGTGGTTGGA
    ACGAGTAGGC TTCGTGACTT ATATGACAAA TTTGAGGAGG AGTTGGGGAG CAGGCAAGAA
    AAGGCCAAAG CTGCTCGGCC TCCGTGGGAA CCTCCAAAGA CGAAGCTCGA TGAAGATTTA
    GAGAGTTCCA GTGAATCCGA GTGTGAGTCT GATGAGGACA GCACCTGTTC TAGCAGCTCA
    GACTCTGAAG TTTTTGACGT TATTGCAGAA ATCAAACGCA AAAAGGCCCA CCCTGACCGA
    CTTCATGATG AACTTTGGTA CAACGATCCA GGCCAGATGA ATGATGGACC ACTCTGCAAA
    TGCAGCGCAA AGGCAAGACG CACAGGAATT AGGCACAGCA TTTATCCTGG AGAAGAGGCC
    ATCAAGCCCT GTCGTCCTAT GACCAACAAT GCTGGCAGAC TTTTCCACTA CCGGATCACA
    GTCTCCCCGC CTACGAACTT TTTAACTGAC AGGCCAACTG TTATAGAATA CGATGATCAC
    GAGTATATCT TTGAAGGATT TTCTATGTTT GCACATGCCC CCCTGACCAA TATTCCACTG
    TGTAAAGTAA TTAGATTCAA CATAGACTAC ACGATTCATT TCATTGAAGA GATGATGCCG
    GAGAATTTTT GTGTGAAAGG GCTTGAACTC TTTTCACTGT TCCTATTCAG AGATATTTTG
    GAATTATATG ACTGGAATCT TAAAGGTCCT TTGTTTGAAG ACAGCCCTCC CTGCTGCCCA
    AGATTTCATT TCATGCCACG TTTTGTAAGA TTTCTTCCAG ATGGAGGAAA GGAAGTGCTG
    TCCATGCACC AGATTCTCCT GTACTTGTTA AGGTGCAGCA AAGCCCTGGT GCCTGAGGAG
    GAGATTGCCA ATATGCTTCA GTGGGAGGAG CTGGAGTGGC AGAAATATGC AGAAGAATGC
    AAAGGCATGA TTGTTACCAA CCCTGGGACG AAACCAAGCT CTGTCCGTAT CGATCAACTG
    GATCGTGAAC AGTTCAACCC CGATGTGATT ACTTTTCCGA TTATCGTCCA CTTTGGGATA
    CGCCCTGCAC AGTACCAAAA ACTGTGGAAG AGTTATGTGA AACTTCGCCA CCTCCTAGCA
    AATAGTCCCA AAGTCAAACA AACTGACAAA CAGAAGCTGG CACAGAGGGA GGAAGCCCTC
    CAAAAAATAC GGCAGAAGAA TACAATGAGA CGAGAAGTAA CGGTGGAGCT AAGTAGCCAA
    GGATTCTGGA AAACTGGCAT CCGTTCTGAT GTCTGTCAGC ATGCAATGAT GCTACCTGTT
    CTGACCCATC ATATCCGCTA CCACCAATGC CTAATGCATT TGGACAAGTT GATAGGATAT
    ACTTTCCAAG ATCGTTGTCT GTTGCAGCTG GCCATGACTC ATCCAAGTCA TCATTTAAAT
    TTTGGAATGA ATCCTGATCA TGCCAGGAAT TCATTATCTA ACTGTGGAAT TCGGCAGCCC
    AAATACGGAG ACAGAAAAGT TCATCACATG CACATGCGGA AGAAAGGGAT TAACACCTTG
    ATAAATATCA TGTCACGCCT TGGCCAAGAT GACCCAACTC CCTCGAGGAT TAACCACAAT
    GAACGGTTGG AATTCCTGGG TGATGCTGTT GTTGAATTTC TGACCAGCGT CCATTTGTAC
    TATTTGTTTC CTAGTCTGGA AGAAGGAGGA TTAGCAACCT ATCGGACTGC CATTGTTCAG
    AATCAGCACC TTGCCATGCT AGCAAAGAAA CTTGAACTGG ATCGATTTAT GCTGTATGCT
    CACGGGCCTG ACCTTTGTAG AGAATCGGAC CTTCGACATG CAATGGCCAA TTGTTTTGAA
    GCGTTAATAG GAGCTGTTTA CTTGGAGGGA AGCCTGGAGG AAGCCAAGCA GTTATTTGGA
    CGCTTGCTCT TTAATGATCC GGACCTGCGC GAAGTCTGGC TCAATTATCC TCTCCACCCA
    CTCCAACTAC AAGAGCCAAA TACTGATCGA CAACTTATTG AAACTTCTCC AGTTCTACAA
    AAACTTACTG AGTTTGAAGA AGCAATTGGA GTAATTTTTA CTCATGTTCG ACTTCTGGCA
    AGGGCATTCA CATTGAGAAC TGTGGGATTT AACCATCTGA CCCTAGGCCA CAATCAGAGA
    ATGGAATTCC TAGGTGACTC CATAATGCAA CTGGTAGCCA CAGAGTACTT ATTCATTCAT
    TTCCCAGATC ATCATGAAGG ACACTTAACT TTGTTGCGAA GCTCTTTGGT GAATAATAGA
    ACTCAGGCCA AGGTAGCGGA GGAGCTGGGC ATGCAGGAGT ACGCCATAAC CAACGACAAG
    ACCAAGAGGC CTGTGGCGCT TCGCACCAAG ACCTTGGCGG ACCTTTTGGA ATCATTTATT
    GCAGCGCTGT ACATTGATAA GGATTTGGAA TATGTTCATA CTTTCATGAA TGTCTGCTTC
    TTTCCACGAT TGAAAGAGTT CATTTTGAAT CAGGATTGGA ATGACCCCAA ATCCCAGCTT
    CAGCAGTGTT GCTTGACACT TAGGACAGAA GGAAAAGAGC CAGACATTCC TCTGTACAAG
    ACTCTGCAGA CAGTGGGCCC ATCCCATGCC CGAACCTACA CTGTGGCTGT TTATTTCAAG
    GGAGAAAGAA TAGGCTGTGG GAAAGGACCA AGTATTCAGC AAGCGGAAAT GGGAGCAGCA
    ATGGATGCGC TTGAAAAATA TAATTTTCCC CAGATGGCCC ATCAGAAGCG GTTCATCGAA
    CGGAAGTACA GACAAGAGTT AAAAGAAATG AGGTGGGAAA GAGAGCATCA AGAGAGAGAG
    CCAGATGAGA CTGAAGACAT CAAGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95849
    Clone ID Related Accession (Same CDS sequence) XM_017009401.1 , XM_017009401.2
    Accession Version XM_017009401.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGTCGCTC CCCAAGTAGG GAGAAGAAGA GAGCTCGTTG GGAGGAAGAA
    AAAGACCGTT GGAGTGACAA CCAGAGTTCT GGCAAAGACA AGAACTATAC CTCAATCAAG
    GAAAAAGAGC CCGAGGAGAC CATGCCTGAC AAGAATGAGG AGGAAGAAGA AGAACTTCTT
    AAGCCTGTGT GGATTCGATG CACTCATTCA GAAAACTACT ACTCCAGTGA CCCCATGGAT
    CAGGTGGGAG ATTCTACAGT GGTTGGAACG AGTAGGCTTC GTGACTTATA TGACAAATTT
    GAGGAGGAGT TGGGGAGCAG GCAAGAAAAG GCCAAAGCTG CTCGGCCTCC GTGGGAACCT
    CCAAAGACGA AGCTCGATGA AGATTTAGAG AGTTCCAGTG AATCCGAGTG TGAGTCTGAT
    GAGGACAGCA CCTGTTCTAG CAGCTCAGAC TCTGAAGTTT TTGACGTTAT TGCAGAAATC
    AAACGCAAAA AGGCCCACCC TGACCGACTT CATGATGAAC TTTGGTACAA CGATCCAGGC
    CAGATGAATG ATGGACCACT CTGCAAATGC AGCGCAAAGG CAAGACGCAC AGGAATTAGG
    CACAGCATTT ATCCTGGAGA AGAGGCCATC AAGCCCTGTC GTCCTATGAC CAACAATGCT
    GGCAGACTTT TCCACTACCG GATCACAGTC TCCCCGCCTA CGAACTTTTT AACTGACAGG
    CCAACTGTTA TAGAATACGA TGATCACGAG TATATCTTTG AAGGATTTTC TATGTTTGCA
    CATGCCCCCC TGACCAATAT TCCACTGTGT AAAGTAATTA GATTCAACAT AGACTACACG
    ATTCATTTCA TTGAAGAGAT GATGCCGGAG AATTTTTGTG TGAAAGGGCT TGAACTCTTT
    TCACTGTTCC TATTCAGAGA TATTTTGGAA TTATATGACT GGAATCTTAA AGGTCCTTTG
    TTTGAAGACA GCCCTCCCTG CTGCCCAAGA TTTCATTTCA TGCCACGTTT TGTAAGATTT
    CTTCCAGATG GAGGAAAGGA AGTGCTGTCC ATGCACCAGA TTCTCCTGTA CTTGTTAAGG
    TGCAGCAAAG CCCTGGTGCC TGAGGAGGAG ATTGCCAATA TGCTTCAGTG GGAGGAGCTG
    GAGTGGCAGA AATATGCAGA AGAATGCAAA GGCATGATTG TTACCAACCC TGGGACGAAA
    CCAAGCTCTG TCCGTATCGA TCAACTGGAT CGTGAACAGT TCAACCCCGA TGTGATTACT
    TTTCCGATTA TCGTCCACTT TGGGATACGC CCTGCACAGT ACCAAAAACT GTGGAAGAGT
    TATGTGAAAC TTCGCCACCT CCTAGCAAAT AGTCCCAAAG TCAAACAAAC TGACAAACAG
    AAGCTGGCAC AGAGGGAGGA AGCCCTCCAA AAAATACGGC AGAAGAATAC AATGAGACGA
    GAAGTAACGG TGGAGCTAAG TAGCCAAGGA TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CAATGATGCT ACCTGTTCTG ACCCATCATA TCCGCTACCA CCAATGCCTA
    ATGCATTTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT GCAGCTGGCC
    ATGACTCATC CAAGTCATCA TTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCA
    TTATCTAACT GTGGAATTCG GCAGCCCAAA TACGGAGACA GAAAAGTTCA TCACATGCAC
    ATGCGGAAGA AAGGGATTAA CACCTTGATA AATATCATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CGAGGATTAA CCACAATGAA CGGTTGGAAT TCCTGGGTGA TGCTGTTGTT
    GAATTTCTGA CCAGCGTCCA TTTGTACTAT TTGTTTCCTA GTCTGGAAGA AGGAGGATTA
    GCAACCTATC GGACTGCCAT TGTTCAGAAT CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GATTTATGCT GTATGCTCAC GGGCCTGACC TTTGTAGAGA ATCGGACCTT
    CGACATGCAA TGGCCAATTG TTTTGAAGCG TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTGCTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAACTACAAG AGCCAAATAC TGATCGACAA
    CTTATTGAAA CTTCTCCAGT TCTACAAAAA CTTACTGAGT TTGAAGAAGC AATTGGAGTA
    ATTTTTACTC ATGTTCGACT TCTGGCAAGG GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGAATG GAATTCCTAG GTGACTCCAT AATGCAACTG
    GTAGCCACAG AGTACTTATT CATTCATTTC CCAGATCATC ATGAAGGACA CTTAACTTTG
    TTGCGAAGCT CTTTGGTGAA TAATAGAACT CAGGCCAAGG TAGCGGAGGA GCTGGGCATG
    CAGGAGTACG CCATAACCAA CGACAAGACC AAGAGGCCTG TGGCGCTTCG CACCAAGACC
    TTGGCGGACC TTTTGGAATC ATTTATTGCA GCGCTGTACA TTGATAAGGA TTTGGAATAT
    GTTCATACTT TCATGAATGT CTGCTTCTTT CCACGATTGA AAGAGTTCAT TTTGAATCAG
    GATTGGAATG ACCCCAAATC CCAGCTTCAG CAGTGTTGCT TGACACTTAG GACAGAAGGA
    AAAGAGCCAG ACATTCCTCT GTACAAGACT CTGCAGACAG TGGGCCCATC CCATGCCCGA
    ACCTACACTG TGGCTGTTTA TTTCAAGGGA GAAAGAATAG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CGGAAATGGG AGCAGCAATG GATGCGCTTG AAAAATATAA TTTTCCCCAG
    ATGGCCCATC AGAAGCGGTT CATCGAACGG AAGTACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAGAGAGCCA GATGAGACTG AAGACATCAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864890.1
    CDS436..4332
    Translation

    Target ORF information:

    RefSeq Version XM_017009401.1
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009401.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGTCGCTC CCCAAGTAGG GAGAAGAAGA GAGCTCGTTG GGAGGAAGAA
    AAAGACCGTT GGAGTGACAA CCAGAGTTCT GGCAAAGACA AGAACTATAC CTCAATCAAG
    GAAAAAGAGC CCGAGGAGAC CATGCCTGAC AAGAATGAGG AGGAAGAAGA AGAACTTCTT
    AAGCCTGTGT GGATTCGATG CACTCATTCA GAAAACTACT ACTCCAGTGA CCCCATGGAT
    CAGGTGGGAG ATTCTACAGT GGTTGGAACG AGTAGGCTTC GTGACTTATA TGACAAATTT
    GAGGAGGAGT TGGGGAGCAG GCAAGAAAAG GCCAAAGCTG CTCGGCCTCC GTGGGAACCT
    CCAAAGACGA AGCTCGATGA AGATTTAGAG AGTTCCAGTG AATCCGAGTG TGAGTCTGAT
    GAGGACAGCA CCTGTTCTAG CAGCTCAGAC TCTGAAGTTT TTGACGTTAT TGCAGAAATC
    AAACGCAAAA AGGCCCACCC TGACCGACTT CATGATGAAC TTTGGTACAA CGATCCAGGC
    CAGATGAATG ATGGACCACT CTGCAAATGC AGCGCAAAGG CAAGACGCAC AGGAATTAGG
    CACAGCATTT ATCCTGGAGA AGAGGCCATC AAGCCCTGTC GTCCTATGAC CAACAATGCT
    GGCAGACTTT TCCACTACCG GATCACAGTC TCCCCGCCTA CGAACTTTTT AACTGACAGG
    CCAACTGTTA TAGAATACGA TGATCACGAG TATATCTTTG AAGGATTTTC TATGTTTGCA
    CATGCCCCCC TGACCAATAT TCCACTGTGT AAAGTAATTA GATTCAACAT AGACTACACG
    ATTCATTTCA TTGAAGAGAT GATGCCGGAG AATTTTTGTG TGAAAGGGCT TGAACTCTTT
    TCACTGTTCC TATTCAGAGA TATTTTGGAA TTATATGACT GGAATCTTAA AGGTCCTTTG
    TTTGAAGACA GCCCTCCCTG CTGCCCAAGA TTTCATTTCA TGCCACGTTT TGTAAGATTT
    CTTCCAGATG GAGGAAAGGA AGTGCTGTCC ATGCACCAGA TTCTCCTGTA CTTGTTAAGG
    TGCAGCAAAG CCCTGGTGCC TGAGGAGGAG ATTGCCAATA TGCTTCAGTG GGAGGAGCTG
    GAGTGGCAGA AATATGCAGA AGAATGCAAA GGCATGATTG TTACCAACCC TGGGACGAAA
    CCAAGCTCTG TCCGTATCGA TCAACTGGAT CGTGAACAGT TCAACCCCGA TGTGATTACT
    TTTCCGATTA TCGTCCACTT TGGGATACGC CCTGCACAGT ACCAAAAACT GTGGAAGAGT
    TATGTGAAAC TTCGCCACCT CCTAGCAAAT AGTCCCAAAG TCAAACAAAC TGACAAACAG
    AAGCTGGCAC AGAGGGAGGA AGCCCTCCAA AAAATACGGC AGAAGAATAC AATGAGACGA
    GAAGTAACGG TGGAGCTAAG TAGCCAAGGA TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CAATGATGCT ACCTGTTCTG ACCCATCATA TCCGCTACCA CCAATGCCTA
    ATGCATTTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT GCAGCTGGCC
    ATGACTCATC CAAGTCATCA TTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCA
    TTATCTAACT GTGGAATTCG GCAGCCCAAA TACGGAGACA GAAAAGTTCA TCACATGCAC
    ATGCGGAAGA AAGGGATTAA CACCTTGATA AATATCATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CGAGGATTAA CCACAATGAA CGGTTGGAAT TCCTGGGTGA TGCTGTTGTT
    GAATTTCTGA CCAGCGTCCA TTTGTACTAT TTGTTTCCTA GTCTGGAAGA AGGAGGATTA
    GCAACCTATC GGACTGCCAT TGTTCAGAAT CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GATTTATGCT GTATGCTCAC GGGCCTGACC TTTGTAGAGA ATCGGACCTT
    CGACATGCAA TGGCCAATTG TTTTGAAGCG TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTGCTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAACTACAAG AGCCAAATAC TGATCGACAA
    CTTATTGAAA CTTCTCCAGT TCTACAAAAA CTTACTGAGT TTGAAGAAGC AATTGGAGTA
    ATTTTTACTC ATGTTCGACT TCTGGCAAGG GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGAATG GAATTCCTAG GTGACTCCAT AATGCAACTG
    GTAGCCACAG AGTACTTATT CATTCATTTC CCAGATCATC ATGAAGGACA CTTAACTTTG
    TTGCGAAGCT CTTTGGTGAA TAATAGAACT CAGGCCAAGG TAGCGGAGGA GCTGGGCATG
    CAGGAGTACG CCATAACCAA CGACAAGACC AAGAGGCCTG TGGCGCTTCG CACCAAGACC
    TTGGCGGACC TTTTGGAATC ATTTATTGCA GCGCTGTACA TTGATAAGGA TTTGGAATAT
    GTTCATACTT TCATGAATGT CTGCTTCTTT CCACGATTGA AAGAGTTCAT TTTGAATCAG
    GATTGGAATG ACCCCAAATC CCAGCTTCAG CAGTGTTGCT TGACACTTAG GACAGAAGGA
    AAAGAGCCAG ACATTCCTCT GTACAAGACT CTGCAGACAG TGGGCCCATC CCATGCCCGA
    ACCTACACTG TGGCTGTTTA TTTCAAGGGA GAAAGAATAG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CGGAAATGGG AGCAGCAATG GATGCGCTTG AAAAATATAA TTTTCCCCAG
    ATGGCCCATC AGAAGCGGTT CATCGAACGG AAGTACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAGAGAGCCA GATGAGACTG AAGACATCAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95849
    Clone ID Related Accession (Same CDS sequence) XM_017009401.1 , XM_017009401.2
    Accession Version XM_017009401.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009401.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGTCGCTC CCCAAGTAGG GAGAAGAAGA GAGCTCGTTG GGAGGAAGAA
    AAAGACCGTT GGAGTGACAA CCAGAGTTCT GGCAAAGACA AGAACTATAC CTCAATCAAG
    GAAAAAGAGC CCGAGGAGAC CATGCCTGAC AAGAATGAGG AGGAAGAAGA AGAACTTCTT
    AAGCCTGTGT GGATTCGATG CACTCATTCA GAAAACTACT ACTCCAGTGA CCCCATGGAT
    CAGGTGGGAG ATTCTACAGT GGTTGGAACG AGTAGGCTTC GTGACTTATA TGACAAATTT
    GAGGAGGAGT TGGGGAGCAG GCAAGAAAAG GCCAAAGCTG CTCGGCCTCC GTGGGAACCT
    CCAAAGACGA AGCTCGATGA AGATTTAGAG AGTTCCAGTG AATCCGAGTG TGAGTCTGAT
    GAGGACAGCA CCTGTTCTAG CAGCTCAGAC TCTGAAGTTT TTGACGTTAT TGCAGAAATC
    AAACGCAAAA AGGCCCACCC TGACCGACTT CATGATGAAC TTTGGTACAA CGATCCAGGC
    CAGATGAATG ATGGACCACT CTGCAAATGC AGCGCAAAGG CAAGACGCAC AGGAATTAGG
    CACAGCATTT ATCCTGGAGA AGAGGCCATC AAGCCCTGTC GTCCTATGAC CAACAATGCT
    GGCAGACTTT TCCACTACCG GATCACAGTC TCCCCGCCTA CGAACTTTTT AACTGACAGG
    CCAACTGTTA TAGAATACGA TGATCACGAG TATATCTTTG AAGGATTTTC TATGTTTGCA
    CATGCCCCCC TGACCAATAT TCCACTGTGT AAAGTAATTA GATTCAACAT AGACTACACG
    ATTCATTTCA TTGAAGAGAT GATGCCGGAG AATTTTTGTG TGAAAGGGCT TGAACTCTTT
    TCACTGTTCC TATTCAGAGA TATTTTGGAA TTATATGACT GGAATCTTAA AGGTCCTTTG
    TTTGAAGACA GCCCTCCCTG CTGCCCAAGA TTTCATTTCA TGCCACGTTT TGTAAGATTT
    CTTCCAGATG GAGGAAAGGA AGTGCTGTCC ATGCACCAGA TTCTCCTGTA CTTGTTAAGG
    TGCAGCAAAG CCCTGGTGCC TGAGGAGGAG ATTGCCAATA TGCTTCAGTG GGAGGAGCTG
    GAGTGGCAGA AATATGCAGA AGAATGCAAA GGCATGATTG TTACCAACCC TGGGACGAAA
    CCAAGCTCTG TCCGTATCGA TCAACTGGAT CGTGAACAGT TCAACCCCGA TGTGATTACT
    TTTCCGATTA TCGTCCACTT TGGGATACGC CCTGCACAGT ACCAAAAACT GTGGAAGAGT
    TATGTGAAAC TTCGCCACCT CCTAGCAAAT AGTCCCAAAG TCAAACAAAC TGACAAACAG
    AAGCTGGCAC AGAGGGAGGA AGCCCTCCAA AAAATACGGC AGAAGAATAC AATGAGACGA
    GAAGTAACGG TGGAGCTAAG TAGCCAAGGA TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CAATGATGCT ACCTGTTCTG ACCCATCATA TCCGCTACCA CCAATGCCTA
    ATGCATTTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT GCAGCTGGCC
    ATGACTCATC CAAGTCATCA TTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCA
    TTATCTAACT GTGGAATTCG GCAGCCCAAA TACGGAGACA GAAAAGTTCA TCACATGCAC
    ATGCGGAAGA AAGGGATTAA CACCTTGATA AATATCATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CGAGGATTAA CCACAATGAA CGGTTGGAAT TCCTGGGTGA TGCTGTTGTT
    GAATTTCTGA CCAGCGTCCA TTTGTACTAT TTGTTTCCTA GTCTGGAAGA AGGAGGATTA
    GCAACCTATC GGACTGCCAT TGTTCAGAAT CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GATTTATGCT GTATGCTCAC GGGCCTGACC TTTGTAGAGA ATCGGACCTT
    CGACATGCAA TGGCCAATTG TTTTGAAGCG TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTGCTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAACTACAAG AGCCAAATAC TGATCGACAA
    CTTATTGAAA CTTCTCCAGT TCTACAAAAA CTTACTGAGT TTGAAGAAGC AATTGGAGTA
    ATTTTTACTC ATGTTCGACT TCTGGCAAGG GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGAATG GAATTCCTAG GTGACTCCAT AATGCAACTG
    GTAGCCACAG AGTACTTATT CATTCATTTC CCAGATCATC ATGAAGGACA CTTAACTTTG
    TTGCGAAGCT CTTTGGTGAA TAATAGAACT CAGGCCAAGG TAGCGGAGGA GCTGGGCATG
    CAGGAGTACG CCATAACCAA CGACAAGACC AAGAGGCCTG TGGCGCTTCG CACCAAGACC
    TTGGCGGACC TTTTGGAATC ATTTATTGCA GCGCTGTACA TTGATAAGGA TTTGGAATAT
    GTTCATACTT TCATGAATGT CTGCTTCTTT CCACGATTGA AAGAGTTCAT TTTGAATCAG
    GATTGGAATG ACCCCAAATC CCAGCTTCAG CAGTGTTGCT TGACACTTAG GACAGAAGGA
    AAAGAGCCAG ACATTCCTCT GTACAAGACT CTGCAGACAG TGGGCCCATC CCATGCCCGA
    ACCTACACTG TGGCTGTTTA TTTCAAGGGA GAAAGAATAG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CGGAAATGGG AGCAGCAATG GATGCGCTTG AAAAATATAA TTTTCCCCAG
    ATGGCCCATC AGAAGCGGTT CATCGAACGG AAGTACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAGAGAGCCA GATGAGACTG AAGACATCAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864890.1
    CDS403..4299
    Translation

    Target ORF information:

    RefSeq Version XM_017009401.2
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009401.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGTCGCTC CCCAAGTAGG GAGAAGAAGA GAGCTCGTTG GGAGGAAGAA
    AAAGACCGTT GGAGTGACAA CCAGAGTTCT GGCAAAGACA AGAACTATAC CTCAATCAAG
    GAAAAAGAGC CCGAGGAGAC CATGCCTGAC AAGAATGAGG AGGAAGAAGA AGAACTTCTT
    AAGCCTGTGT GGATTCGATG CACTCATTCA GAAAACTACT ACTCCAGTGA CCCCATGGAT
    CAGGTGGGAG ATTCTACAGT GGTTGGAACG AGTAGGCTTC GTGACTTATA TGACAAATTT
    GAGGAGGAGT TGGGGAGCAG GCAAGAAAAG GCCAAAGCTG CTCGGCCTCC GTGGGAACCT
    CCAAAGACGA AGCTCGATGA AGATTTAGAG AGTTCCAGTG AATCCGAGTG TGAGTCTGAT
    GAGGACAGCA CCTGTTCTAG CAGCTCAGAC TCTGAAGTTT TTGACGTTAT TGCAGAAATC
    AAACGCAAAA AGGCCCACCC TGACCGACTT CATGATGAAC TTTGGTACAA CGATCCAGGC
    CAGATGAATG ATGGACCACT CTGCAAATGC AGCGCAAAGG CAAGACGCAC AGGAATTAGG
    CACAGCATTT ATCCTGGAGA AGAGGCCATC AAGCCCTGTC GTCCTATGAC CAACAATGCT
    GGCAGACTTT TCCACTACCG GATCACAGTC TCCCCGCCTA CGAACTTTTT AACTGACAGG
    CCAACTGTTA TAGAATACGA TGATCACGAG TATATCTTTG AAGGATTTTC TATGTTTGCA
    CATGCCCCCC TGACCAATAT TCCACTGTGT AAAGTAATTA GATTCAACAT AGACTACACG
    ATTCATTTCA TTGAAGAGAT GATGCCGGAG AATTTTTGTG TGAAAGGGCT TGAACTCTTT
    TCACTGTTCC TATTCAGAGA TATTTTGGAA TTATATGACT GGAATCTTAA AGGTCCTTTG
    TTTGAAGACA GCCCTCCCTG CTGCCCAAGA TTTCATTTCA TGCCACGTTT TGTAAGATTT
    CTTCCAGATG GAGGAAAGGA AGTGCTGTCC ATGCACCAGA TTCTCCTGTA CTTGTTAAGG
    TGCAGCAAAG CCCTGGTGCC TGAGGAGGAG ATTGCCAATA TGCTTCAGTG GGAGGAGCTG
    GAGTGGCAGA AATATGCAGA AGAATGCAAA GGCATGATTG TTACCAACCC TGGGACGAAA
    CCAAGCTCTG TCCGTATCGA TCAACTGGAT CGTGAACAGT TCAACCCCGA TGTGATTACT
    TTTCCGATTA TCGTCCACTT TGGGATACGC CCTGCACAGT ACCAAAAACT GTGGAAGAGT
    TATGTGAAAC TTCGCCACCT CCTAGCAAAT AGTCCCAAAG TCAAACAAAC TGACAAACAG
    AAGCTGGCAC AGAGGGAGGA AGCCCTCCAA AAAATACGGC AGAAGAATAC AATGAGACGA
    GAAGTAACGG TGGAGCTAAG TAGCCAAGGA TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CAATGATGCT ACCTGTTCTG ACCCATCATA TCCGCTACCA CCAATGCCTA
    ATGCATTTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT GCAGCTGGCC
    ATGACTCATC CAAGTCATCA TTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCA
    TTATCTAACT GTGGAATTCG GCAGCCCAAA TACGGAGACA GAAAAGTTCA TCACATGCAC
    ATGCGGAAGA AAGGGATTAA CACCTTGATA AATATCATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CGAGGATTAA CCACAATGAA CGGTTGGAAT TCCTGGGTGA TGCTGTTGTT
    GAATTTCTGA CCAGCGTCCA TTTGTACTAT TTGTTTCCTA GTCTGGAAGA AGGAGGATTA
    GCAACCTATC GGACTGCCAT TGTTCAGAAT CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GATTTATGCT GTATGCTCAC GGGCCTGACC TTTGTAGAGA ATCGGACCTT
    CGACATGCAA TGGCCAATTG TTTTGAAGCG TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTGCTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAACTACAAG AGCCAAATAC TGATCGACAA
    CTTATTGAAA CTTCTCCAGT TCTACAAAAA CTTACTGAGT TTGAAGAAGC AATTGGAGTA
    ATTTTTACTC ATGTTCGACT TCTGGCAAGG GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGAATG GAATTCCTAG GTGACTCCAT AATGCAACTG
    GTAGCCACAG AGTACTTATT CATTCATTTC CCAGATCATC ATGAAGGACA CTTAACTTTG
    TTGCGAAGCT CTTTGGTGAA TAATAGAACT CAGGCCAAGG TAGCGGAGGA GCTGGGCATG
    CAGGAGTACG CCATAACCAA CGACAAGACC AAGAGGCCTG TGGCGCTTCG CACCAAGACC
    TTGGCGGACC TTTTGGAATC ATTTATTGCA GCGCTGTACA TTGATAAGGA TTTGGAATAT
    GTTCATACTT TCATGAATGT CTGCTTCTTT CCACGATTGA AAGAGTTCAT TTTGAATCAG
    GATTGGAATG ACCCCAAATC CCAGCTTCAG CAGTGTTGCT TGACACTTAG GACAGAAGGA
    AAAGAGCCAG ACATTCCTCT GTACAAGACT CTGCAGACAG TGGGCCCATC CCATGCCCGA
    ACCTACACTG TGGCTGTTTA TTTCAAGGGA GAAAGAATAG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CGGAAATGGG AGCAGCAATG GATGCGCTTG AAAAATATAA TTTTCCCCAG
    ATGGCCCATC AGAAGCGGTT CATCGAACGG AAGTACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAGAGAGCCA GATGAGACTG AAGACATCAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39316
    Clone ID Related Accession (Same CDS sequence) XM_005248294.3 , XM_005248294.4
    Accession Version XM_005248294.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248294.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGTCGCTC CCCAAGTAGG GAGAAGAAGA GAGCTCGTTG GGAGGAAGAA
    AAAGACCGTT GGAGTGACAA CCAGAGTTCT GGCAAAGACA AGAACTATAC CTCAATCAAG
    GAAAAAGAGC CCGAGGAGAC CATGCCTGAC AAGAATGAGG AGGAAGAAGA AGAACTTCTT
    AAGCCTGTGT GGATTCGATG CACTCATTCA GAAAACTACT ACTCCAGTGA CCCCATGGAT
    CAGGTGGGAG ATTCTACAGT GGTTGGAACG AGTAGGCTTC GTGACTTATA TGACAAATTT
    GAGGAGGAGT TGGGGAGCAG GCAAGAAAAG GCCAAAGCTG CTCGGCCTCC GTGGGAACCT
    CCAAAGACGA AGCTCGATGA AGATTTAGAG AGTTCCAGTG AATCCGAGTG TGAGTCTGAT
    GAGGACAGCA CCTGTTCTAG CAGCTCAGAC TCTGAAGTTT TTGACGTTAT TGCAGAAATC
    AAACGCAAAA AGGCCCACCC TGACCGACTT CATGATGAAC TTTGGTACAA CGATCCAGGC
    CAGATGAATG ATGGACCACT CTGCAAATGC AGCGCAAAGG CAAGACGCAC AGGAATTAGG
    CACAGCATTT ATCCTGGAGA AGAGGCCATC AAGCCCTGTC GTCCTATGAC CAACAATGCT
    GGCAGACTTT TCCACTACCG GATCACAGTC TCCCCGCCTA CGAACTTTTT AACTGACAGG
    CCAACTGTTA TAGAATACGA TGATCACGAG TATATCTTTG AAGGATTTTC TATGTTTGCA
    CATGCCCCCC TGACCAATAT TCCACTGTGT AAAGTAATTA GATTCAACAT AGACTACACG
    ATTCATTTCA TTGAAGAGAT GATGCCGGAG AATTTTTGTG TGAAAGGGCT TGAACTCTTT
    TCACTGTTCC TATTCAGAGA TATTTTGGAA TTATATGACT GGAATCTTAA AGGTCCTTTG
    TTTGAAGACA GCCCTCCCTG CTGCCCAAGA TTTCATTTCA TGCCACGTTT TGTAAGATTT
    CTTCCAGATG GAGGAAAGGA AGTGCTGTCC ATGCACCAGA TTCTCCTGTA CTTGTTAAGG
    TGCAGCAAAG CCCTGGTGCC TGAGGAGGAG ATTGCCAATA TGCTTCAGTG GGAGGAGCTG
    GAGTGGCAGA AATATGCAGA AGAATGCAAA GGCATGATTG TTACCAACCC TGGGACGAAA
    CCAAGCTCTG TCCGTATCGA TCAACTGGAT CGTGAACAGT TCAACCCCGA TGTGATTACT
    TTTCCGATTA TCGTCCACTT TGGGATACGC CCTGCACAGT TGAGTTATGC AGGAGACCCA
    CAGTACCAAA AACTGTGGAA GAGTTATGTG AAACTTCGCC ACCTCCTAGC AAATAGTCCC
    AAAGTCAAAC AAACTGACAA ACAGAAGCTG GCACAGAGGG AGGAAGCCCT CCAAAAAATA
    CGGCAGAAGA ATACAATGAG ACGAGAAGTA ACGGTGGAGC TAAGTAGCCA AGGATTCTGG
    AAAACTGGCA TCCGTTCTGA TGTCTGTCAG CATGCAATGA TGCTACCTGT TCTGACCCAT
    CATATCCGCT ACCACCAATG CCTAATGCAT TTGGACAAGT TGATAGGATA TACTTTCCAA
    GATCGTTGTC TGTTGCAGCT GGCCATGACT CATCCAAGTC ATCATTTAAA TTTTGGAATG
    AATCCTGATC ATGCCAGGAA TTCATTATCT AACTGTGGAA TTCGGCAGCC CAAATACGGA
    GACAGAAAAG TTCATCACAT GCACATGCGG AAGAAAGGGA TTAACACCTT GATAAATATC
    ATGTCACGCC TTGGCCAAGA TGACCCAACT CCCTCGAGGA TTAACCACAA TGAACGGTTG
    GAATTCCTGG GTGATGCTGT TGTTGAATTT CTGACCAGCG TCCATTTGTA CTATTTGTTT
    CCTAGTCTGG AAGAAGGAGG ATTAGCAACC TATCGGACTG CCATTGTTCA GAATCAGCAC
    CTTGCCATGC TAGCAAAGAA ACTTGAACTG GATCGATTTA TGCTGTATGC TCACGGGCCT
    GACCTTTGTA GAGAATCGGA CCTTCGACAT GCAATGGCCA ATTGTTTTGA AGCGTTAATA
    GGAGCTGTTT ACTTGGAGGG AAGCCTGGAG GAAGCCAAGC AGTTATTTGG ACGCTTGCTC
    TTTAATGATC CGGACCTGCG CGAAGTCTGG CTCAATTATC CTCTCCACCC ACTCCAACTA
    CAAGAGCCAA ATACTGATCG ACAACTTATT GAAACTTCTC CAGTTCTACA AAAACTTACT
    GAGTTTGAAG AAGCAATTGG AGTAATTTTT ACTCATGTTC GACTTCTGGC AAGGGCATTC
    ACATTGAGAA CTGTGGGATT TAACCATCTG ACCCTAGGCC ACAATCAGAG AATGGAATTC
    CTAGGTGACT CCATAATGCA ACTGGTAGCC ACAGAGTACT TATTCATTCA TTTCCCAGAT
    CATCATGAAG GACACTTAAC TTTGTTGCGA AGCTCTTTGG TGAATAATAG AACTCAGGCC
    AAGGTAGCGG AGGAGCTGGG CATGCAGGAG TACGCCATAA CCAACGACAA GACCAAGAGG
    CCTGTGGCGC TTCGCACCAA GACCTTGGCG GACCTTTTGG AATCATTTAT TGCAGCGCTG
    TACATTGATA AGGATTTGGA ATATGTTCAT ACTTTCATGA ATGTCTGCTT CTTTCCACGA
    TTGAAAGAGT TCATTTTGAA TCAGGATTGG AATGACCCCA AATCCCAGCT TCAGCAGTGT
    TGCTTGACAC TTAGGACAGA AGGAAAAGAG CCAGACATTC CTCTGTACAA GACTCTGCAG
    ACAGTGGGCC CATCCCATGC CCGAACCTAC ACTGTGGCTG TTTATTTCAA GGGAGAAAGA
    ATAGGCTGTG GGAAAGGACC AAGTATTCAG CAAGCGGAAA TGGGAGCAGC AATGGATGCG
    CTTGAAAAAT ATAATTTTCC CCAGATGGCC CATCAGAAGC GGTTCATCGA ACGGAAGTAC
    AGACAAGAGT TAAAAGAAAT GAGGTGGGAA AGAGAGCATC AAGAGAGAGA GCCAGATGAG
    ACTGAAGACA TCAAGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248351.1
    CDS436..4356
    Translation

    Target ORF information:

    RefSeq Version XM_005248294.3
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248294.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGTCGCTC CCCAAGTAGG GAGAAGAAGA GAGCTCGTTG GGAGGAAGAA
    AAAGACCGTT GGAGTGACAA CCAGAGTTCT GGCAAAGACA AGAACTATAC CTCAATCAAG
    GAAAAAGAGC CCGAGGAGAC CATGCCTGAC AAGAATGAGG AGGAAGAAGA AGAACTTCTT
    AAGCCTGTGT GGATTCGATG CACTCATTCA GAAAACTACT ACTCCAGTGA CCCCATGGAT
    CAGGTGGGAG ATTCTACAGT GGTTGGAACG AGTAGGCTTC GTGACTTATA TGACAAATTT
    GAGGAGGAGT TGGGGAGCAG GCAAGAAAAG GCCAAAGCTG CTCGGCCTCC GTGGGAACCT
    CCAAAGACGA AGCTCGATGA AGATTTAGAG AGTTCCAGTG AATCCGAGTG TGAGTCTGAT
    GAGGACAGCA CCTGTTCTAG CAGCTCAGAC TCTGAAGTTT TTGACGTTAT TGCAGAAATC
    AAACGCAAAA AGGCCCACCC TGACCGACTT CATGATGAAC TTTGGTACAA CGATCCAGGC
    CAGATGAATG ATGGACCACT CTGCAAATGC AGCGCAAAGG CAAGACGCAC AGGAATTAGG
    CACAGCATTT ATCCTGGAGA AGAGGCCATC AAGCCCTGTC GTCCTATGAC CAACAATGCT
    GGCAGACTTT TCCACTACCG GATCACAGTC TCCCCGCCTA CGAACTTTTT AACTGACAGG
    CCAACTGTTA TAGAATACGA TGATCACGAG TATATCTTTG AAGGATTTTC TATGTTTGCA
    CATGCCCCCC TGACCAATAT TCCACTGTGT AAAGTAATTA GATTCAACAT AGACTACACG
    ATTCATTTCA TTGAAGAGAT GATGCCGGAG AATTTTTGTG TGAAAGGGCT TGAACTCTTT
    TCACTGTTCC TATTCAGAGA TATTTTGGAA TTATATGACT GGAATCTTAA AGGTCCTTTG
    TTTGAAGACA GCCCTCCCTG CTGCCCAAGA TTTCATTTCA TGCCACGTTT TGTAAGATTT
    CTTCCAGATG GAGGAAAGGA AGTGCTGTCC ATGCACCAGA TTCTCCTGTA CTTGTTAAGG
    TGCAGCAAAG CCCTGGTGCC TGAGGAGGAG ATTGCCAATA TGCTTCAGTG GGAGGAGCTG
    GAGTGGCAGA AATATGCAGA AGAATGCAAA GGCATGATTG TTACCAACCC TGGGACGAAA
    CCAAGCTCTG TCCGTATCGA TCAACTGGAT CGTGAACAGT TCAACCCCGA TGTGATTACT
    TTTCCGATTA TCGTCCACTT TGGGATACGC CCTGCACAGT TGAGTTATGC AGGAGACCCA
    CAGTACCAAA AACTGTGGAA GAGTTATGTG AAACTTCGCC ACCTCCTAGC AAATAGTCCC
    AAAGTCAAAC AAACTGACAA ACAGAAGCTG GCACAGAGGG AGGAAGCCCT CCAAAAAATA
    CGGCAGAAGA ATACAATGAG ACGAGAAGTA ACGGTGGAGC TAAGTAGCCA AGGATTCTGG
    AAAACTGGCA TCCGTTCTGA TGTCTGTCAG CATGCAATGA TGCTACCTGT TCTGACCCAT
    CATATCCGCT ACCACCAATG CCTAATGCAT TTGGACAAGT TGATAGGATA TACTTTCCAA
    GATCGTTGTC TGTTGCAGCT GGCCATGACT CATCCAAGTC ATCATTTAAA TTTTGGAATG
    AATCCTGATC ATGCCAGGAA TTCATTATCT AACTGTGGAA TTCGGCAGCC CAAATACGGA
    GACAGAAAAG TTCATCACAT GCACATGCGG AAGAAAGGGA TTAACACCTT GATAAATATC
    ATGTCACGCC TTGGCCAAGA TGACCCAACT CCCTCGAGGA TTAACCACAA TGAACGGTTG
    GAATTCCTGG GTGATGCTGT TGTTGAATTT CTGACCAGCG TCCATTTGTA CTATTTGTTT
    CCTAGTCTGG AAGAAGGAGG ATTAGCAACC TATCGGACTG CCATTGTTCA GAATCAGCAC
    CTTGCCATGC TAGCAAAGAA ACTTGAACTG GATCGATTTA TGCTGTATGC TCACGGGCCT
    GACCTTTGTA GAGAATCGGA CCTTCGACAT GCAATGGCCA ATTGTTTTGA AGCGTTAATA
    GGAGCTGTTT ACTTGGAGGG AAGCCTGGAG GAAGCCAAGC AGTTATTTGG ACGCTTGCTC
    TTTAATGATC CGGACCTGCG CGAAGTCTGG CTCAATTATC CTCTCCACCC ACTCCAACTA
    CAAGAGCCAA ATACTGATCG ACAACTTATT GAAACTTCTC CAGTTCTACA AAAACTTACT
    GAGTTTGAAG AAGCAATTGG AGTAATTTTT ACTCATGTTC GACTTCTGGC AAGGGCATTC
    ACATTGAGAA CTGTGGGATT TAACCATCTG ACCCTAGGCC ACAATCAGAG AATGGAATTC
    CTAGGTGACT CCATAATGCA ACTGGTAGCC ACAGAGTACT TATTCATTCA TTTCCCAGAT
    CATCATGAAG GACACTTAAC TTTGTTGCGA AGCTCTTTGG TGAATAATAG AACTCAGGCC
    AAGGTAGCGG AGGAGCTGGG CATGCAGGAG TACGCCATAA CCAACGACAA GACCAAGAGG
    CCTGTGGCGC TTCGCACCAA GACCTTGGCG GACCTTTTGG AATCATTTAT TGCAGCGCTG
    TACATTGATA AGGATTTGGA ATATGTTCAT ACTTTCATGA ATGTCTGCTT CTTTCCACGA
    TTGAAAGAGT TCATTTTGAA TCAGGATTGG AATGACCCCA AATCCCAGCT TCAGCAGTGT
    TGCTTGACAC TTAGGACAGA AGGAAAAGAG CCAGACATTC CTCTGTACAA GACTCTGCAG
    ACAGTGGGCC CATCCCATGC CCGAACCTAC ACTGTGGCTG TTTATTTCAA GGGAGAAAGA
    ATAGGCTGTG GGAAAGGACC AAGTATTCAG CAAGCGGAAA TGGGAGCAGC AATGGATGCG
    CTTGAAAAAT ATAATTTTCC CCAGATGGCC CATCAGAAGC GGTTCATCGA ACGGAAGTAC
    AGACAAGAGT TAAAAGAAAT GAGGTGGGAA AGAGAGCATC AAGAGAGAGA GCCAGATGAG
    ACTGAAGACA TCAAGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39316
    Clone ID Related Accession (Same CDS sequence) XM_005248294.3 , XM_005248294.4
    Accession Version XM_005248294.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248294.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGTCGCTC CCCAAGTAGG GAGAAGAAGA GAGCTCGTTG GGAGGAAGAA
    AAAGACCGTT GGAGTGACAA CCAGAGTTCT GGCAAAGACA AGAACTATAC CTCAATCAAG
    GAAAAAGAGC CCGAGGAGAC CATGCCTGAC AAGAATGAGG AGGAAGAAGA AGAACTTCTT
    AAGCCTGTGT GGATTCGATG CACTCATTCA GAAAACTACT ACTCCAGTGA CCCCATGGAT
    CAGGTGGGAG ATTCTACAGT GGTTGGAACG AGTAGGCTTC GTGACTTATA TGACAAATTT
    GAGGAGGAGT TGGGGAGCAG GCAAGAAAAG GCCAAAGCTG CTCGGCCTCC GTGGGAACCT
    CCAAAGACGA AGCTCGATGA AGATTTAGAG AGTTCCAGTG AATCCGAGTG TGAGTCTGAT
    GAGGACAGCA CCTGTTCTAG CAGCTCAGAC TCTGAAGTTT TTGACGTTAT TGCAGAAATC
    AAACGCAAAA AGGCCCACCC TGACCGACTT CATGATGAAC TTTGGTACAA CGATCCAGGC
    CAGATGAATG ATGGACCACT CTGCAAATGC AGCGCAAAGG CAAGACGCAC AGGAATTAGG
    CACAGCATTT ATCCTGGAGA AGAGGCCATC AAGCCCTGTC GTCCTATGAC CAACAATGCT
    GGCAGACTTT TCCACTACCG GATCACAGTC TCCCCGCCTA CGAACTTTTT AACTGACAGG
    CCAACTGTTA TAGAATACGA TGATCACGAG TATATCTTTG AAGGATTTTC TATGTTTGCA
    CATGCCCCCC TGACCAATAT TCCACTGTGT AAAGTAATTA GATTCAACAT AGACTACACG
    ATTCATTTCA TTGAAGAGAT GATGCCGGAG AATTTTTGTG TGAAAGGGCT TGAACTCTTT
    TCACTGTTCC TATTCAGAGA TATTTTGGAA TTATATGACT GGAATCTTAA AGGTCCTTTG
    TTTGAAGACA GCCCTCCCTG CTGCCCAAGA TTTCATTTCA TGCCACGTTT TGTAAGATTT
    CTTCCAGATG GAGGAAAGGA AGTGCTGTCC ATGCACCAGA TTCTCCTGTA CTTGTTAAGG
    TGCAGCAAAG CCCTGGTGCC TGAGGAGGAG ATTGCCAATA TGCTTCAGTG GGAGGAGCTG
    GAGTGGCAGA AATATGCAGA AGAATGCAAA GGCATGATTG TTACCAACCC TGGGACGAAA
    CCAAGCTCTG TCCGTATCGA TCAACTGGAT CGTGAACAGT TCAACCCCGA TGTGATTACT
    TTTCCGATTA TCGTCCACTT TGGGATACGC CCTGCACAGT TGAGTTATGC AGGAGACCCA
    CAGTACCAAA AACTGTGGAA GAGTTATGTG AAACTTCGCC ACCTCCTAGC AAATAGTCCC
    AAAGTCAAAC AAACTGACAA ACAGAAGCTG GCACAGAGGG AGGAAGCCCT CCAAAAAATA
    CGGCAGAAGA ATACAATGAG ACGAGAAGTA ACGGTGGAGC TAAGTAGCCA AGGATTCTGG
    AAAACTGGCA TCCGTTCTGA TGTCTGTCAG CATGCAATGA TGCTACCTGT TCTGACCCAT
    CATATCCGCT ACCACCAATG CCTAATGCAT TTGGACAAGT TGATAGGATA TACTTTCCAA
    GATCGTTGTC TGTTGCAGCT GGCCATGACT CATCCAAGTC ATCATTTAAA TTTTGGAATG
    AATCCTGATC ATGCCAGGAA TTCATTATCT AACTGTGGAA TTCGGCAGCC CAAATACGGA
    GACAGAAAAG TTCATCACAT GCACATGCGG AAGAAAGGGA TTAACACCTT GATAAATATC
    ATGTCACGCC TTGGCCAAGA TGACCCAACT CCCTCGAGGA TTAACCACAA TGAACGGTTG
    GAATTCCTGG GTGATGCTGT TGTTGAATTT CTGACCAGCG TCCATTTGTA CTATTTGTTT
    CCTAGTCTGG AAGAAGGAGG ATTAGCAACC TATCGGACTG CCATTGTTCA GAATCAGCAC
    CTTGCCATGC TAGCAAAGAA ACTTGAACTG GATCGATTTA TGCTGTATGC TCACGGGCCT
    GACCTTTGTA GAGAATCGGA CCTTCGACAT GCAATGGCCA ATTGTTTTGA AGCGTTAATA
    GGAGCTGTTT ACTTGGAGGG AAGCCTGGAG GAAGCCAAGC AGTTATTTGG ACGCTTGCTC
    TTTAATGATC CGGACCTGCG CGAAGTCTGG CTCAATTATC CTCTCCACCC ACTCCAACTA
    CAAGAGCCAA ATACTGATCG ACAACTTATT GAAACTTCTC CAGTTCTACA AAAACTTACT
    GAGTTTGAAG AAGCAATTGG AGTAATTTTT ACTCATGTTC GACTTCTGGC AAGGGCATTC
    ACATTGAGAA CTGTGGGATT TAACCATCTG ACCCTAGGCC ACAATCAGAG AATGGAATTC
    CTAGGTGACT CCATAATGCA ACTGGTAGCC ACAGAGTACT TATTCATTCA TTTCCCAGAT
    CATCATGAAG GACACTTAAC TTTGTTGCGA AGCTCTTTGG TGAATAATAG AACTCAGGCC
    AAGGTAGCGG AGGAGCTGGG CATGCAGGAG TACGCCATAA CCAACGACAA GACCAAGAGG
    CCTGTGGCGC TTCGCACCAA GACCTTGGCG GACCTTTTGG AATCATTTAT TGCAGCGCTG
    TACATTGATA AGGATTTGGA ATATGTTCAT ACTTTCATGA ATGTCTGCTT CTTTCCACGA
    TTGAAAGAGT TCATTTTGAA TCAGGATTGG AATGACCCCA AATCCCAGCT TCAGCAGTGT
    TGCTTGACAC TTAGGACAGA AGGAAAAGAG CCAGACATTC CTCTGTACAA GACTCTGCAG
    ACAGTGGGCC CATCCCATGC CCGAACCTAC ACTGTGGCTG TTTATTTCAA GGGAGAAAGA
    ATAGGCTGTG GGAAAGGACC AAGTATTCAG CAAGCGGAAA TGGGAGCAGC AATGGATGCG
    CTTGAAAAAT ATAATTTTCC CCAGATGGCC CATCAGAAGC GGTTCATCGA ACGGAAGTAC
    AGACAAGAGT TAAAAGAAAT GAGGTGGGAA AGAGAGCATC AAGAGAGAGA GCCAGATGAG
    ACTGAAGACA TCAAGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248351.1
    CDS403..4323
    Translation

    Target ORF information:

    RefSeq Version XM_005248294.4
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248294.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGTCGCTC CCCAAGTAGG GAGAAGAAGA GAGCTCGTTG GGAGGAAGAA
    AAAGACCGTT GGAGTGACAA CCAGAGTTCT GGCAAAGACA AGAACTATAC CTCAATCAAG
    GAAAAAGAGC CCGAGGAGAC CATGCCTGAC AAGAATGAGG AGGAAGAAGA AGAACTTCTT
    AAGCCTGTGT GGATTCGATG CACTCATTCA GAAAACTACT ACTCCAGTGA CCCCATGGAT
    CAGGTGGGAG ATTCTACAGT GGTTGGAACG AGTAGGCTTC GTGACTTATA TGACAAATTT
    GAGGAGGAGT TGGGGAGCAG GCAAGAAAAG GCCAAAGCTG CTCGGCCTCC GTGGGAACCT
    CCAAAGACGA AGCTCGATGA AGATTTAGAG AGTTCCAGTG AATCCGAGTG TGAGTCTGAT
    GAGGACAGCA CCTGTTCTAG CAGCTCAGAC TCTGAAGTTT TTGACGTTAT TGCAGAAATC
    AAACGCAAAA AGGCCCACCC TGACCGACTT CATGATGAAC TTTGGTACAA CGATCCAGGC
    CAGATGAATG ATGGACCACT CTGCAAATGC AGCGCAAAGG CAAGACGCAC AGGAATTAGG
    CACAGCATTT ATCCTGGAGA AGAGGCCATC AAGCCCTGTC GTCCTATGAC CAACAATGCT
    GGCAGACTTT TCCACTACCG GATCACAGTC TCCCCGCCTA CGAACTTTTT AACTGACAGG
    CCAACTGTTA TAGAATACGA TGATCACGAG TATATCTTTG AAGGATTTTC TATGTTTGCA
    CATGCCCCCC TGACCAATAT TCCACTGTGT AAAGTAATTA GATTCAACAT AGACTACACG
    ATTCATTTCA TTGAAGAGAT GATGCCGGAG AATTTTTGTG TGAAAGGGCT TGAACTCTTT
    TCACTGTTCC TATTCAGAGA TATTTTGGAA TTATATGACT GGAATCTTAA AGGTCCTTTG
    TTTGAAGACA GCCCTCCCTG CTGCCCAAGA TTTCATTTCA TGCCACGTTT TGTAAGATTT
    CTTCCAGATG GAGGAAAGGA AGTGCTGTCC ATGCACCAGA TTCTCCTGTA CTTGTTAAGG
    TGCAGCAAAG CCCTGGTGCC TGAGGAGGAG ATTGCCAATA TGCTTCAGTG GGAGGAGCTG
    GAGTGGCAGA AATATGCAGA AGAATGCAAA GGCATGATTG TTACCAACCC TGGGACGAAA
    CCAAGCTCTG TCCGTATCGA TCAACTGGAT CGTGAACAGT TCAACCCCGA TGTGATTACT
    TTTCCGATTA TCGTCCACTT TGGGATACGC CCTGCACAGT TGAGTTATGC AGGAGACCCA
    CAGTACCAAA AACTGTGGAA GAGTTATGTG AAACTTCGCC ACCTCCTAGC AAATAGTCCC
    AAAGTCAAAC AAACTGACAA ACAGAAGCTG GCACAGAGGG AGGAAGCCCT CCAAAAAATA
    CGGCAGAAGA ATACAATGAG ACGAGAAGTA ACGGTGGAGC TAAGTAGCCA AGGATTCTGG
    AAAACTGGCA TCCGTTCTGA TGTCTGTCAG CATGCAATGA TGCTACCTGT TCTGACCCAT
    CATATCCGCT ACCACCAATG CCTAATGCAT TTGGACAAGT TGATAGGATA TACTTTCCAA
    GATCGTTGTC TGTTGCAGCT GGCCATGACT CATCCAAGTC ATCATTTAAA TTTTGGAATG
    AATCCTGATC ATGCCAGGAA TTCATTATCT AACTGTGGAA TTCGGCAGCC CAAATACGGA
    GACAGAAAAG TTCATCACAT GCACATGCGG AAGAAAGGGA TTAACACCTT GATAAATATC
    ATGTCACGCC TTGGCCAAGA TGACCCAACT CCCTCGAGGA TTAACCACAA TGAACGGTTG
    GAATTCCTGG GTGATGCTGT TGTTGAATTT CTGACCAGCG TCCATTTGTA CTATTTGTTT
    CCTAGTCTGG AAGAAGGAGG ATTAGCAACC TATCGGACTG CCATTGTTCA GAATCAGCAC
    CTTGCCATGC TAGCAAAGAA ACTTGAACTG GATCGATTTA TGCTGTATGC TCACGGGCCT
    GACCTTTGTA GAGAATCGGA CCTTCGACAT GCAATGGCCA ATTGTTTTGA AGCGTTAATA
    GGAGCTGTTT ACTTGGAGGG AAGCCTGGAG GAAGCCAAGC AGTTATTTGG ACGCTTGCTC
    TTTAATGATC CGGACCTGCG CGAAGTCTGG CTCAATTATC CTCTCCACCC ACTCCAACTA
    CAAGAGCCAA ATACTGATCG ACAACTTATT GAAACTTCTC CAGTTCTACA AAAACTTACT
    GAGTTTGAAG AAGCAATTGG AGTAATTTTT ACTCATGTTC GACTTCTGGC AAGGGCATTC
    ACATTGAGAA CTGTGGGATT TAACCATCTG ACCCTAGGCC ACAATCAGAG AATGGAATTC
    CTAGGTGACT CCATAATGCA ACTGGTAGCC ACAGAGTACT TATTCATTCA TTTCCCAGAT
    CATCATGAAG GACACTTAAC TTTGTTGCGA AGCTCTTTGG TGAATAATAG AACTCAGGCC
    AAGGTAGCGG AGGAGCTGGG CATGCAGGAG TACGCCATAA CCAACGACAA GACCAAGAGG
    CCTGTGGCGC TTCGCACCAA GACCTTGGCG GACCTTTTGG AATCATTTAT TGCAGCGCTG
    TACATTGATA AGGATTTGGA ATATGTTCAT ACTTTCATGA ATGTCTGCTT CTTTCCACGA
    TTGAAAGAGT TCATTTTGAA TCAGGATTGG AATGACCCCA AATCCCAGCT TCAGCAGTGT
    TGCTTGACAC TTAGGACAGA AGGAAAAGAG CCAGACATTC CTCTGTACAA GACTCTGCAG
    ACAGTGGGCC CATCCCATGC CCGAACCTAC ACTGTGGCTG TTTATTTCAA GGGAGAAAGA
    ATAGGCTGTG GGAAAGGACC AAGTATTCAG CAAGCGGAAA TGGGAGCAGC AATGGATGCG
    CTTGAAAAAT ATAATTTTCC CCAGATGGCC CATCAGAAGC GGTTCATCGA ACGGAAGTAC
    AGACAAGAGT TAAAAGAAAT GAGGTGGGAA AGAGAGCATC AAGAGAGAGA GCCAGATGAG
    ACTGAAGACA TCAAGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu67332
    Clone ID Related Accession (Same CDS sequence) XM_011514033.3 , XM_011514033.2
    Accession Version XM_011514033.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514033.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATGGG ATCTCAGGAT TGGTGTTGAA AATGGCAAGA CCCAAGAATG TGCGTATTCT
    GGCATCATAT CTAATCTTCA AGTAATATTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512335.1
    CDS403..4155
    Translation

    Target ORF information:

    RefSeq Version XM_011514033.3
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514033.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATGGG ATCTCAGGAT TGGTGTTGAA AATGGCAAGA CCCAAGAATG TGCGTATTCT
    GGCATCATAT CTAATCTTCA AGTAATATTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu67332
    Clone ID Related Accession (Same CDS sequence) XM_011514033.3 , XM_011514033.2
    Accession Version XM_011514033.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514033.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATGGG ATCTCAGGAT TGGTGTTGAA AATGGCAAGA CCCAAGAATG TGCGTATTCT
    GGCATCATAT CTAATCTTCA AGTAATATTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512335.1
    CDS436..4188
    Translation

    Target ORF information:

    RefSeq Version XM_011514033.2
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514033.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATGGG ATCTCAGGAT TGGTGTTGAA AATGGCAAGA CCCAAGAATG TGCGTATTCT
    GGCATCATAT CTAATCTTCA AGTAATATTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16375
    Clone ID Related Accession (Same CDS sequence) NM_001100412.1 , XM_024446035.1 , NM_001100412.2
    Accession Version NM_001100412.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-29
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB065470.1, BX647724.1, BC024261.1, AC008768.8 and AA917625.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647724.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGTCG CTCCCCAAGT
    AGGGAGAAGA AGAGAGCTCG TTGGGAGGAA GAAAAAGACC GTTGGAGTGA CAACCAGAGT
    TCTGGCAAAG ACAAGAACTA TACCTCAATC AAGGAAAAAG AGCCCGAGGA GACCATGCCT
    GACAAGAATG AGGAGGAAGA AGAAGAACTT CTTAAGCCTG TGTGGATTCG ATGCACTCAT
    TCAGAAAACT ACTACTCCAG TGACCCCATG GATCAGGTGG GAGATTCTAC AGTGGTTGGA
    ACGAGTAGGC TTCGTGACTT ATATGACAAA TTTGAGGAGG AGTTGGGGAG CAGGCAAGAA
    AAGGCCAAAG CTGCTCGGCC TCCGTGGGAA CCTCCAAAGA CGAAGCTCGA TGAAGATTTA
    GAGAGTTCCA GTGAATCCGA GTGTGAGTCT GATGAGGACA GCACCTGTTC TAGCAGCTCA
    GACTCTGAAG TTTTTGACGT TATTGCAGAA ATCAAACGCA AAAAGGCCCA CCCTGACCGA
    CTTCATGATG AACTTTGGTA CAACGATCCA GGCCAGATGA ATGATGGACC ACTCTGCAAA
    TGCAGCGCAA AGGCAAGACG CACAGGAATT AGGCACAGCA TTTATCCTGG AGAAGAGGCC
    ATCAAGCCCT GTCGTCCTAT GACCAACAAT GCTGGCAGAC TTTTCCACTA CCGGATCACA
    GTCTCCCCGC CTACGAACTT TTTAACTGAC AGGCCAACTG TTATAGAATA CGATGATCAC
    GAGTATATCT TTGAAGGATT TTCTATGTTT GCACATGCCC CCCTGACCAA TATTCCACTG
    TGTAAAGTAA TTAGATTCAA CATAGACTAC ACGATTCATT TCATTGAAGA GATGATGCCG
    GAGAATTTTT GTGTGAAAGG GCTTGAACTC TTTTCACTGT TCCTATTCAG AGATATTTTG
    GAATTATATG ACTGGAATCT TAAAGGTCCT TTGTTTGAAG ACAGCCCTCC CTGCTGCCCA
    AGATTTCATT TCATGCCACG TTTTGTAAGA TTTCTTCCAG ATGGAGGAAA GGAAGTGCTG
    TCCATGCACC AGATTCTCCT GTACTTGTTA AGGTGCAGCA AAGCCCTGGT GCCTGAGGAG
    GAGATTGCCA ATATGCTTCA GTGGGAGGAG CTGGAGTGGC AGAAATATGC AGAAGAATGC
    AAAGGCATGA TTGTTACCAA CCCTGGGACG AAACCAAGCT CTGTCCGTAT CGATCAACTG
    GATCGTGAAC AGTTCAACCC CGATGTGATT ACTTTTCCGA TTATCGTCCA CTTTGGGATA
    CGCCCTGCAC AGTTGAGTTA TGCAGGAGAC CCACAGTACC AAAAACTGTG GAAGAGTTAT
    GTGAAACTTC GCCACCTCCT AGCAAATAGT CCCAAAGTCA AACAAACTGA CAAACAGAAG
    CTGGCACAGA GGGAGGAAGC CCTCCAAAAA ATACGGCAGA AGAATACAAT GAGACGAGAA
    GTAACGGTGG AGCTAAGTAG CCAAGGATTC TGGAAAACTG GCATCCGTTC TGATGTCTGT
    CAGCATGCAA TGATGCTACC TGTTCTGACC CATCATATCC GCTACCACCA ATGCCTAATG
    CATTTGGACA AGTTGATAGG ATATACTTTC CAAGATCGTT GTCTGTTGCA GCTGGCCATG
    ACTCATCCAA GTCATCATTT AAATTTTGGA ATGAATCCTG ATCATGCCAG GAATTCATTA
    TCTAACTGTG GAATTCGGCA GCCCAAATAC GGAGACAGAA AAGTTCATCA CATGCACATG
    CGGAAGAAAG GGATTAACAC CTTGATAAAT ATCATGTCAC GCCTTGGCCA AGATGACCCA
    ACTCCCTCGA GGATTAACCA CAATGAACGG TTGGAATTCC TGGGTGATGC TGTTGTTGAA
    TTTCTGACCA GCGTCCATTT GTACTATTTG TTTCCTAGTC TGGAAGAAGG AGGATTAGCA
    ACCTATCGGA CTGCCATTGT TCAGAATCAG CACCTTGCCA TGCTAGCAAA GAAACTTGAA
    CTGGATCGAT TTATGCTGTA TGCTCACGGG CCTGACCTTT GTAGAGAATC GGACCTTCGA
    CATGCAATGG CCAATTGTTT TGAAGCGTTA ATAGGAGCTG TTTACTTGGA GGGAAGCCTG
    GAGGAAGCCA AGCAGTTATT TGGACGCTTG CTCTTTAATG ATCCGGACCT GCGCGAAGTC
    TGGCTCAATT ATCCTCTCCA CCCACTCCAA CTACAAGAGC CAAATACTGA TCGACAACTT
    ATTGAAACTT CTCCAGTTCT ACAAAAACTT ACTGAGTTTG AAGAAGCAAT TGGAGTAATT
    TTTACTCATG TTCGACTTCT GGCAAGGGCA TTCACATTGA GAACTGTGGG ATTTAACCAT
    CTGACCCTAG GCCACAATCA GAGAATGGAA TTCCTAGGTG ACTCCATAAT GCAACTGGTA
    GCCACAGAGT ACTTATTCAT TCATTTCCCA GATCATCATG AAGGACACTT AACTTTGTTG
    CGAAGCTCTT TGGTGAATAA TAGAACTCAG GCCAAGGTAG CGGAGGAGCT GGGCATGCAG
    GAGTACGCCA TAACCAACGA CAAGACCAAG AGGCCTGTGG CGCTTCGCAC CAAGACCTTG
    GCGGACCTTT TGGAATCATT TATTGCAGCG CTGTACATTG ATAAGGATTT GGAATATGTT
    CATACTTTCA TGAATGTCTG CTTCTTTCCA CGATTGAAAG AGTTCATTTT GAATCAGGAT
    TGGAATGACC CCAAATCCCA GCTTCAGCAG TGTTGCTTGA CACTTAGGAC AGAAGGAAAA
    GAGCCAGACA TTCCTCTGTA CAAGACTCTG CAGACAGTGG GCCCATCCCA TGCCCGAACC
    TACACTGTGG CTGTTTATTT CAAGGGAGAA AGAATAGGCT GTGGGAAAGG ACCAAGTATT
    CAGCAAGCGG AAATGGGAGC AGCAATGGAT GCGCTTGAAA AATATAATTT TCCCCAGATG
    GCCCATCAGA AGCGGTTCAT CGAACGGAAG TACAGACAAG AGTTAAAAGA AATGAGGTGG
    GAAAGAGAGC ATCAAGAGAG AGAGCCAGAT GAGACTGAAG ACATCAAGAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001093882.1
    CDS436..4449
    Translation

    Target ORF information:

    RefSeq Version NM_001100412.1
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001100412.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGTCG CTCCCCAAGT
    AGGGAGAAGA AGAGAGCTCG TTGGGAGGAA GAAAAAGACC GTTGGAGTGA CAACCAGAGT
    TCTGGCAAAG ACAAGAACTA TACCTCAATC AAGGAAAAAG AGCCCGAGGA GACCATGCCT
    GACAAGAATG AGGAGGAAGA AGAAGAACTT CTTAAGCCTG TGTGGATTCG ATGCACTCAT
    TCAGAAAACT ACTACTCCAG TGACCCCATG GATCAGGTGG GAGATTCTAC AGTGGTTGGA
    ACGAGTAGGC TTCGTGACTT ATATGACAAA TTTGAGGAGG AGTTGGGGAG CAGGCAAGAA
    AAGGCCAAAG CTGCTCGGCC TCCGTGGGAA CCTCCAAAGA CGAAGCTCGA TGAAGATTTA
    GAGAGTTCCA GTGAATCCGA GTGTGAGTCT GATGAGGACA GCACCTGTTC TAGCAGCTCA
    GACTCTGAAG TTTTTGACGT TATTGCAGAA ATCAAACGCA AAAAGGCCCA CCCTGACCGA
    CTTCATGATG AACTTTGGTA CAACGATCCA GGCCAGATGA ATGATGGACC ACTCTGCAAA
    TGCAGCGCAA AGGCAAGACG CACAGGAATT AGGCACAGCA TTTATCCTGG AGAAGAGGCC
    ATCAAGCCCT GTCGTCCTAT GACCAACAAT GCTGGCAGAC TTTTCCACTA CCGGATCACA
    GTCTCCCCGC CTACGAACTT TTTAACTGAC AGGCCAACTG TTATAGAATA CGATGATCAC
    GAGTATATCT TTGAAGGATT TTCTATGTTT GCACATGCCC CCCTGACCAA TATTCCACTG
    TGTAAAGTAA TTAGATTCAA CATAGACTAC ACGATTCATT TCATTGAAGA GATGATGCCG
    GAGAATTTTT GTGTGAAAGG GCTTGAACTC TTTTCACTGT TCCTATTCAG AGATATTTTG
    GAATTATATG ACTGGAATCT TAAAGGTCCT TTGTTTGAAG ACAGCCCTCC CTGCTGCCCA
    AGATTTCATT TCATGCCACG TTTTGTAAGA TTTCTTCCAG ATGGAGGAAA GGAAGTGCTG
    TCCATGCACC AGATTCTCCT GTACTTGTTA AGGTGCAGCA AAGCCCTGGT GCCTGAGGAG
    GAGATTGCCA ATATGCTTCA GTGGGAGGAG CTGGAGTGGC AGAAATATGC AGAAGAATGC
    AAAGGCATGA TTGTTACCAA CCCTGGGACG AAACCAAGCT CTGTCCGTAT CGATCAACTG
    GATCGTGAAC AGTTCAACCC CGATGTGATT ACTTTTCCGA TTATCGTCCA CTTTGGGATA
    CGCCCTGCAC AGTTGAGTTA TGCAGGAGAC CCACAGTACC AAAAACTGTG GAAGAGTTAT
    GTGAAACTTC GCCACCTCCT AGCAAATAGT CCCAAAGTCA AACAAACTGA CAAACAGAAG
    CTGGCACAGA GGGAGGAAGC CCTCCAAAAA ATACGGCAGA AGAATACAAT GAGACGAGAA
    GTAACGGTGG AGCTAAGTAG CCAAGGATTC TGGAAAACTG GCATCCGTTC TGATGTCTGT
    CAGCATGCAA TGATGCTACC TGTTCTGACC CATCATATCC GCTACCACCA ATGCCTAATG
    CATTTGGACA AGTTGATAGG ATATACTTTC CAAGATCGTT GTCTGTTGCA GCTGGCCATG
    ACTCATCCAA GTCATCATTT AAATTTTGGA ATGAATCCTG ATCATGCCAG GAATTCATTA
    TCTAACTGTG GAATTCGGCA GCCCAAATAC GGAGACAGAA AAGTTCATCA CATGCACATG
    CGGAAGAAAG GGATTAACAC CTTGATAAAT ATCATGTCAC GCCTTGGCCA AGATGACCCA
    ACTCCCTCGA GGATTAACCA CAATGAACGG TTGGAATTCC TGGGTGATGC TGTTGTTGAA
    TTTCTGACCA GCGTCCATTT GTACTATTTG TTTCCTAGTC TGGAAGAAGG AGGATTAGCA
    ACCTATCGGA CTGCCATTGT TCAGAATCAG CACCTTGCCA TGCTAGCAAA GAAACTTGAA
    CTGGATCGAT TTATGCTGTA TGCTCACGGG CCTGACCTTT GTAGAGAATC GGACCTTCGA
    CATGCAATGG CCAATTGTTT TGAAGCGTTA ATAGGAGCTG TTTACTTGGA GGGAAGCCTG
    GAGGAAGCCA AGCAGTTATT TGGACGCTTG CTCTTTAATG ATCCGGACCT GCGCGAAGTC
    TGGCTCAATT ATCCTCTCCA CCCACTCCAA CTACAAGAGC CAAATACTGA TCGACAACTT
    ATTGAAACTT CTCCAGTTCT ACAAAAACTT ACTGAGTTTG AAGAAGCAAT TGGAGTAATT
    TTTACTCATG TTCGACTTCT GGCAAGGGCA TTCACATTGA GAACTGTGGG ATTTAACCAT
    CTGACCCTAG GCCACAATCA GAGAATGGAA TTCCTAGGTG ACTCCATAAT GCAACTGGTA
    GCCACAGAGT ACTTATTCAT TCATTTCCCA GATCATCATG AAGGACACTT AACTTTGTTG
    CGAAGCTCTT TGGTGAATAA TAGAACTCAG GCCAAGGTAG CGGAGGAGCT GGGCATGCAG
    GAGTACGCCA TAACCAACGA CAAGACCAAG AGGCCTGTGG CGCTTCGCAC CAAGACCTTG
    GCGGACCTTT TGGAATCATT TATTGCAGCG CTGTACATTG ATAAGGATTT GGAATATGTT
    CATACTTTCA TGAATGTCTG CTTCTTTCCA CGATTGAAAG AGTTCATTTT GAATCAGGAT
    TGGAATGACC CCAAATCCCA GCTTCAGCAG TGTTGCTTGA CACTTAGGAC AGAAGGAAAA
    GAGCCAGACA TTCCTCTGTA CAAGACTCTG CAGACAGTGG GCCCATCCCA TGCCCGAACC
    TACACTGTGG CTGTTTATTT CAAGGGAGAA AGAATAGGCT GTGGGAAAGG ACCAAGTATT
    CAGCAAGCGG AAATGGGAGC AGCAATGGAT GCGCTTGAAA AATATAATTT TCCCCAGATG
    GCCCATCAGA AGCGGTTCAT CGAACGGAAG TACAGACAAG AGTTAAAAGA AATGAGGTGG
    GAAAGAGAGC ATCAAGAGAG AGAGCCAGAT GAGACTGAAG ACATCAAGAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16375
    Clone ID Related Accession (Same CDS sequence) NM_001100412.1 , XM_024446035.1 , NM_001100412.2
    Accession Version NM_001100412.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-09
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB065470.1, BX647724.1, BC024261.1, AC008768.8 and AA917625.1. On Jun 2, 2019 this sequence version replaced NM_001100412.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647724.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGTCG CTCCCCAAGT
    AGGGAGAAGA AGAGAGCTCG TTGGGAGGAA GAAAAAGACC GTTGGAGTGA CAACCAGAGT
    TCTGGCAAAG ACAAGAACTA TACCTCAATC AAGGAAAAAG AGCCCGAGGA GACCATGCCT
    GACAAGAATG AGGAGGAAGA AGAAGAACTT CTTAAGCCTG TGTGGATTCG ATGCACTCAT
    TCAGAAAACT ACTACTCCAG TGACCCCATG GATCAGGTGG GAGATTCTAC AGTGGTTGGA
    ACGAGTAGGC TTCGTGACTT ATATGACAAA TTTGAGGAGG AGTTGGGGAG CAGGCAAGAA
    AAGGCCAAAG CTGCTCGGCC TCCGTGGGAA CCTCCAAAGA CGAAGCTCGA TGAAGATTTA
    GAGAGTTCCA GTGAATCCGA GTGTGAGTCT GATGAGGACA GCACCTGTTC TAGCAGCTCA
    GACTCTGAAG TTTTTGACGT TATTGCAGAA ATCAAACGCA AAAAGGCCCA CCCTGACCGA
    CTTCATGATG AACTTTGGTA CAACGATCCA GGCCAGATGA ATGATGGACC ACTCTGCAAA
    TGCAGCGCAA AGGCAAGACG CACAGGAATT AGGCACAGCA TTTATCCTGG AGAAGAGGCC
    ATCAAGCCCT GTCGTCCTAT GACCAACAAT GCTGGCAGAC TTTTCCACTA CCGGATCACA
    GTCTCCCCGC CTACGAACTT TTTAACTGAC AGGCCAACTG TTATAGAATA CGATGATCAC
    GAGTATATCT TTGAAGGATT TTCTATGTTT GCACATGCCC CCCTGACCAA TATTCCACTG
    TGTAAAGTAA TTAGATTCAA CATAGACTAC ACGATTCATT TCATTGAAGA GATGATGCCG
    GAGAATTTTT GTGTGAAAGG GCTTGAACTC TTTTCACTGT TCCTATTCAG AGATATTTTG
    GAATTATATG ACTGGAATCT TAAAGGTCCT TTGTTTGAAG ACAGCCCTCC CTGCTGCCCA
    AGATTTCATT TCATGCCACG TTTTGTAAGA TTTCTTCCAG ATGGAGGAAA GGAAGTGCTG
    TCCATGCACC AGATTCTCCT GTACTTGTTA AGGTGCAGCA AAGCCCTGGT GCCTGAGGAG
    GAGATTGCCA ATATGCTTCA GTGGGAGGAG CTGGAGTGGC AGAAATATGC AGAAGAATGC
    AAAGGCATGA TTGTTACCAA CCCTGGGACG AAACCAAGCT CTGTCCGTAT CGATCAACTG
    GATCGTGAAC AGTTCAACCC CGATGTGATT ACTTTTCCGA TTATCGTCCA CTTTGGGATA
    CGCCCTGCAC AGTTGAGTTA TGCAGGAGAC CCACAGTACC AAAAACTGTG GAAGAGTTAT
    GTGAAACTTC GCCACCTCCT AGCAAATAGT CCCAAAGTCA AACAAACTGA CAAACAGAAG
    CTGGCACAGA GGGAGGAAGC CCTCCAAAAA ATACGGCAGA AGAATACAAT GAGACGAGAA
    GTAACGGTGG AGCTAAGTAG CCAAGGATTC TGGAAAACTG GCATCCGTTC TGATGTCTGT
    CAGCATGCAA TGATGCTACC TGTTCTGACC CATCATATCC GCTACCACCA ATGCCTAATG
    CATTTGGACA AGTTGATAGG ATATACTTTC CAAGATCGTT GTCTGTTGCA GCTGGCCATG
    ACTCATCCAA GTCATCATTT AAATTTTGGA ATGAATCCTG ATCATGCCAG GAATTCATTA
    TCTAACTGTG GAATTCGGCA GCCCAAATAC GGAGACAGAA AAGTTCATCA CATGCACATG
    CGGAAGAAAG GGATTAACAC CTTGATAAAT ATCATGTCAC GCCTTGGCCA AGATGACCCA
    ACTCCCTCGA GGATTAACCA CAATGAACGG TTGGAATTCC TGGGTGATGC TGTTGTTGAA
    TTTCTGACCA GCGTCCATTT GTACTATTTG TTTCCTAGTC TGGAAGAAGG AGGATTAGCA
    ACCTATCGGA CTGCCATTGT TCAGAATCAG CACCTTGCCA TGCTAGCAAA GAAACTTGAA
    CTGGATCGAT TTATGCTGTA TGCTCACGGG CCTGACCTTT GTAGAGAATC GGACCTTCGA
    CATGCAATGG CCAATTGTTT TGAAGCGTTA ATAGGAGCTG TTTACTTGGA GGGAAGCCTG
    GAGGAAGCCA AGCAGTTATT TGGACGCTTG CTCTTTAATG ATCCGGACCT GCGCGAAGTC
    TGGCTCAATT ATCCTCTCCA CCCACTCCAA CTACAAGAGC CAAATACTGA TCGACAACTT
    ATTGAAACTT CTCCAGTTCT ACAAAAACTT ACTGAGTTTG AAGAAGCAAT TGGAGTAATT
    TTTACTCATG TTCGACTTCT GGCAAGGGCA TTCACATTGA GAACTGTGGG ATTTAACCAT
    CTGACCCTAG GCCACAATCA GAGAATGGAA TTCCTAGGTG ACTCCATAAT GCAACTGGTA
    GCCACAGAGT ACTTATTCAT TCATTTCCCA GATCATCATG AAGGACACTT AACTTTGTTG
    CGAAGCTCTT TGGTGAATAA TAGAACTCAG GCCAAGGTAG CGGAGGAGCT GGGCATGCAG
    GAGTACGCCA TAACCAACGA CAAGACCAAG AGGCCTGTGG CGCTTCGCAC CAAGACCTTG
    GCGGACCTTT TGGAATCATT TATTGCAGCG CTGTACATTG ATAAGGATTT GGAATATGTT
    CATACTTTCA TGAATGTCTG CTTCTTTCCA CGATTGAAAG AGTTCATTTT GAATCAGGAT
    TGGAATGACC CCAAATCCCA GCTTCAGCAG TGTTGCTTGA CACTTAGGAC AGAAGGAAAA
    GAGCCAGACA TTCCTCTGTA CAAGACTCTG CAGACAGTGG GCCCATCCCA TGCCCGAACC
    TACACTGTGG CTGTTTATTT CAAGGGAGAA AGAATAGGCT GTGGGAAAGG ACCAAGTATT
    CAGCAAGCGG AAATGGGAGC AGCAATGGAT GCGCTTGAAA AATATAATTT TCCCCAGATG
    GCCCATCAGA AGCGGTTCAT CGAACGGAAG TACAGACAAG AGTTAAAAGA AATGAGGTGG
    GAAAGAGAGC ATCAAGAGAG AGAGCCAGAT GAGACTGAAG ACATCAAGAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001093882.1
    CDS354..4367
    Translation

    Target ORF information:

    RefSeq Version NM_001100412.2
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001100412.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGTCG CTCCCCAAGT
    AGGGAGAAGA AGAGAGCTCG TTGGGAGGAA GAAAAAGACC GTTGGAGTGA CAACCAGAGT
    TCTGGCAAAG ACAAGAACTA TACCTCAATC AAGGAAAAAG AGCCCGAGGA GACCATGCCT
    GACAAGAATG AGGAGGAAGA AGAAGAACTT CTTAAGCCTG TGTGGATTCG ATGCACTCAT
    TCAGAAAACT ACTACTCCAG TGACCCCATG GATCAGGTGG GAGATTCTAC AGTGGTTGGA
    ACGAGTAGGC TTCGTGACTT ATATGACAAA TTTGAGGAGG AGTTGGGGAG CAGGCAAGAA
    AAGGCCAAAG CTGCTCGGCC TCCGTGGGAA CCTCCAAAGA CGAAGCTCGA TGAAGATTTA
    GAGAGTTCCA GTGAATCCGA GTGTGAGTCT GATGAGGACA GCACCTGTTC TAGCAGCTCA
    GACTCTGAAG TTTTTGACGT TATTGCAGAA ATCAAACGCA AAAAGGCCCA CCCTGACCGA
    CTTCATGATG AACTTTGGTA CAACGATCCA GGCCAGATGA ATGATGGACC ACTCTGCAAA
    TGCAGCGCAA AGGCAAGACG CACAGGAATT AGGCACAGCA TTTATCCTGG AGAAGAGGCC
    ATCAAGCCCT GTCGTCCTAT GACCAACAAT GCTGGCAGAC TTTTCCACTA CCGGATCACA
    GTCTCCCCGC CTACGAACTT TTTAACTGAC AGGCCAACTG TTATAGAATA CGATGATCAC
    GAGTATATCT TTGAAGGATT TTCTATGTTT GCACATGCCC CCCTGACCAA TATTCCACTG
    TGTAAAGTAA TTAGATTCAA CATAGACTAC ACGATTCATT TCATTGAAGA GATGATGCCG
    GAGAATTTTT GTGTGAAAGG GCTTGAACTC TTTTCACTGT TCCTATTCAG AGATATTTTG
    GAATTATATG ACTGGAATCT TAAAGGTCCT TTGTTTGAAG ACAGCCCTCC CTGCTGCCCA
    AGATTTCATT TCATGCCACG TTTTGTAAGA TTTCTTCCAG ATGGAGGAAA GGAAGTGCTG
    TCCATGCACC AGATTCTCCT GTACTTGTTA AGGTGCAGCA AAGCCCTGGT GCCTGAGGAG
    GAGATTGCCA ATATGCTTCA GTGGGAGGAG CTGGAGTGGC AGAAATATGC AGAAGAATGC
    AAAGGCATGA TTGTTACCAA CCCTGGGACG AAACCAAGCT CTGTCCGTAT CGATCAACTG
    GATCGTGAAC AGTTCAACCC CGATGTGATT ACTTTTCCGA TTATCGTCCA CTTTGGGATA
    CGCCCTGCAC AGTTGAGTTA TGCAGGAGAC CCACAGTACC AAAAACTGTG GAAGAGTTAT
    GTGAAACTTC GCCACCTCCT AGCAAATAGT CCCAAAGTCA AACAAACTGA CAAACAGAAG
    CTGGCACAGA GGGAGGAAGC CCTCCAAAAA ATACGGCAGA AGAATACAAT GAGACGAGAA
    GTAACGGTGG AGCTAAGTAG CCAAGGATTC TGGAAAACTG GCATCCGTTC TGATGTCTGT
    CAGCATGCAA TGATGCTACC TGTTCTGACC CATCATATCC GCTACCACCA ATGCCTAATG
    CATTTGGACA AGTTGATAGG ATATACTTTC CAAGATCGTT GTCTGTTGCA GCTGGCCATG
    ACTCATCCAA GTCATCATTT AAATTTTGGA ATGAATCCTG ATCATGCCAG GAATTCATTA
    TCTAACTGTG GAATTCGGCA GCCCAAATAC GGAGACAGAA AAGTTCATCA CATGCACATG
    CGGAAGAAAG GGATTAACAC CTTGATAAAT ATCATGTCAC GCCTTGGCCA AGATGACCCA
    ACTCCCTCGA GGATTAACCA CAATGAACGG TTGGAATTCC TGGGTGATGC TGTTGTTGAA
    TTTCTGACCA GCGTCCATTT GTACTATTTG TTTCCTAGTC TGGAAGAAGG AGGATTAGCA
    ACCTATCGGA CTGCCATTGT TCAGAATCAG CACCTTGCCA TGCTAGCAAA GAAACTTGAA
    CTGGATCGAT TTATGCTGTA TGCTCACGGG CCTGACCTTT GTAGAGAATC GGACCTTCGA
    CATGCAATGG CCAATTGTTT TGAAGCGTTA ATAGGAGCTG TTTACTTGGA GGGAAGCCTG
    GAGGAAGCCA AGCAGTTATT TGGACGCTTG CTCTTTAATG ATCCGGACCT GCGCGAAGTC
    TGGCTCAATT ATCCTCTCCA CCCACTCCAA CTACAAGAGC CAAATACTGA TCGACAACTT
    ATTGAAACTT CTCCAGTTCT ACAAAAACTT ACTGAGTTTG AAGAAGCAAT TGGAGTAATT
    TTTACTCATG TTCGACTTCT GGCAAGGGCA TTCACATTGA GAACTGTGGG ATTTAACCAT
    CTGACCCTAG GCCACAATCA GAGAATGGAA TTCCTAGGTG ACTCCATAAT GCAACTGGTA
    GCCACAGAGT ACTTATTCAT TCATTTCCCA GATCATCATG AAGGACACTT AACTTTGTTG
    CGAAGCTCTT TGGTGAATAA TAGAACTCAG GCCAAGGTAG CGGAGGAGCT GGGCATGCAG
    GAGTACGCCA TAACCAACGA CAAGACCAAG AGGCCTGTGG CGCTTCGCAC CAAGACCTTG
    GCGGACCTTT TGGAATCATT TATTGCAGCG CTGTACATTG ATAAGGATTT GGAATATGTT
    CATACTTTCA TGAATGTCTG CTTCTTTCCA CGATTGAAAG AGTTCATTTT GAATCAGGAT
    TGGAATGACC CCAAATCCCA GCTTCAGCAG TGTTGCTTGA CACTTAGGAC AGAAGGAAAA
    GAGCCAGACA TTCCTCTGTA CAAGACTCTG CAGACAGTGG GCCCATCCCA TGCCCGAACC
    TACACTGTGG CTGTTTATTT CAAGGGAGAA AGAATAGGCT GTGGGAAAGG ACCAAGTATT
    CAGCAAGCGG AAATGGGAGC AGCAATGGAT GCGCTTGAAA AATATAATTT TCCCCAGATG
    GCCCATCAGA AGCGGTTCAT CGAACGGAAG TACAGACAAG AGTTAAAAGA AATGAGGTGG
    GAAAGAGAGC ATCAAGAGAG AGAGCCAGAT GAGACTGAAG ACATCAAGAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16612
    Clone ID Related Accession (Same CDS sequence) XM_005248291.3 , NM_013235.5 , XM_005248291.4 , XM_024446034.1 , NM_013235.4 , XM_024446033.1
    Accession Version NM_013235.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-09
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB065470.1, AF189011.1, BC024261.1, AC008768.8 and AA917625.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_013235.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF189011.1, SRR1660803.220317.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATCAT TTATTGCAGC GCTGTACATT GATAAGGATT TGGAATATGT TCATACTTTC
    ATGAATGTCT GCTTCTTTCC ACGATTGAAA GAGTTCATTT TGAATCAGGA TTGGAATGAC
    CCCAAATCCC AGCTTCAGCA GTGTTGCTTG ACACTTAGGA CAGAAGGAAA AGAGCCAGAC
    ATTCCTCTGT ACAAGACTCT GCAGACAGTG GGCCCATCCC ATGCCCGAAC CTACACTGTG
    GCTGTTTATT TCAAGGGAGA AAGAATAGGC TGTGGGAAAG GACCAAGTAT TCAGCAAGCG
    GAAATGGGAG CAGCAATGGA TGCGCTTGAA AAATATAATT TTCCCCAGAT GGCCCATCAG
    AAGCGGTTCA TCGAACGGAA GTACAGACAA GAGTTAAAAG AAATGAGGTG GGAAAGAGAG
    CATCAAGAGA GAGAGCCAGA TGAGACTGAA GACATCAAGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_037367.3
    CDS278..4402
    Translation

    Target ORF information:

    RefSeq Version NM_013235.5
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_013235.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATCAT TTATTGCAGC GCTGTACATT GATAAGGATT TGGAATATGT TCATACTTTC
    ATGAATGTCT GCTTCTTTCC ACGATTGAAA GAGTTCATTT TGAATCAGGA TTGGAATGAC
    CCCAAATCCC AGCTTCAGCA GTGTTGCTTG ACACTTAGGA CAGAAGGAAA AGAGCCAGAC
    ATTCCTCTGT ACAAGACTCT GCAGACAGTG GGCCCATCCC ATGCCCGAAC CTACACTGTG
    GCTGTTTATT TCAAGGGAGA AAGAATAGGC TGTGGGAAAG GACCAAGTAT TCAGCAAGCG
    GAAATGGGAG CAGCAATGGA TGCGCTTGAA AAATATAATT TTCCCCAGAT GGCCCATCAG
    AAGCGGTTCA TCGAACGGAA GTACAGACAA GAGTTAAAAG AAATGAGGTG GGAAAGAGAG
    CATCAAGAGA GAGAGCCAGA TGAGACTGAA GACATCAAGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16612
    Clone ID Related Accession (Same CDS sequence) XM_005248291.3 , NM_013235.5 , XM_005248291.4 , XM_024446034.1 , NM_013235.4 , XM_024446033.1
    Accession Version NM_013235.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-09
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB065470.1, AF189011.1, BC024261.1, AC008768.8 and AA917625.1. This sequence is a reference standard in the RefSeqGene project. On Aug 10, 2007 this sequence version replaced NM_013235.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF189011.1, SRR1660803.220317.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATCAT TTATTGCAGC GCTGTACATT GATAAGGATT TGGAATATGT TCATACTTTC
    ATGAATGTCT GCTTCTTTCC ACGATTGAAA GAGTTCATTT TGAATCAGGA TTGGAATGAC
    CCCAAATCCC AGCTTCAGCA GTGTTGCTTG ACACTTAGGA CAGAAGGAAA AGAGCCAGAC
    ATTCCTCTGT ACAAGACTCT GCAGACAGTG GGCCCATCCC ATGCCCGAAC CTACACTGTG
    GCTGTTTATT TCAAGGGAGA AAGAATAGGC TGTGGGAAAG GACCAAGTAT TCAGCAAGCG
    GAAATGGGAG CAGCAATGGA TGCGCTTGAA AAATATAATT TTCCCCAGAT GGCCCATCAG
    AAGCGGTTCA TCGAACGGAA GTACAGACAA GAGTTAAAAG AAATGAGGTG GGAAAGAGAG
    CATCAAGAGA GAGAGCCAGA TGAGACTGAA GACATCAAGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_037367.3
    CDS360..4484
    Translation

    Target ORF information:

    RefSeq Version NM_013235.4
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_013235.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATCAT TTATTGCAGC GCTGTACATT GATAAGGATT TGGAATATGT TCATACTTTC
    ATGAATGTCT GCTTCTTTCC ACGATTGAAA GAGTTCATTT TGAATCAGGA TTGGAATGAC
    CCCAAATCCC AGCTTCAGCA GTGTTGCTTG ACACTTAGGA CAGAAGGAAA AGAGCCAGAC
    ATTCCTCTGT ACAAGACTCT GCAGACAGTG GGCCCATCCC ATGCCCGAAC CTACACTGTG
    GCTGTTTATT TCAAGGGAGA AAGAATAGGC TGTGGGAAAG GACCAAGTAT TCAGCAAGCG
    GAAATGGGAG CAGCAATGGA TGCGCTTGAA AAATATAATT TTCCCCAGAT GGCCCATCAG
    AAGCGGTTCA TCGAACGGAA GTACAGACAA GAGTTAAAAG AAATGAGGTG GGAAAGAGAG
    CATCAAGAGA GAGAGCCAGA TGAGACTGAA GACATCAAGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16375
    Clone ID Related Accession (Same CDS sequence) NM_001100412.1 , XM_024446035.1 , NM_001100412.2
    Accession Version XM_024446035.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGTCG CTCCCCAAGT
    AGGGAGAAGA AGAGAGCTCG TTGGGAGGAA GAAAAAGACC GTTGGAGTGA CAACCAGAGT
    TCTGGCAAAG ACAAGAACTA TACCTCAATC AAGGAAAAAG AGCCCGAGGA GACCATGCCT
    GACAAGAATG AGGAGGAAGA AGAAGAACTT CTTAAGCCTG TGTGGATTCG ATGCACTCAT
    TCAGAAAACT ACTACTCCAG TGACCCCATG GATCAGGTGG GAGATTCTAC AGTGGTTGGA
    ACGAGTAGGC TTCGTGACTT ATATGACAAA TTTGAGGAGG AGTTGGGGAG CAGGCAAGAA
    AAGGCCAAAG CTGCTCGGCC TCCGTGGGAA CCTCCAAAGA CGAAGCTCGA TGAAGATTTA
    GAGAGTTCCA GTGAATCCGA GTGTGAGTCT GATGAGGACA GCACCTGTTC TAGCAGCTCA
    GACTCTGAAG TTTTTGACGT TATTGCAGAA ATCAAACGCA AAAAGGCCCA CCCTGACCGA
    CTTCATGATG AACTTTGGTA CAACGATCCA GGCCAGATGA ATGATGGACC ACTCTGCAAA
    TGCAGCGCAA AGGCAAGACG CACAGGAATT AGGCACAGCA TTTATCCTGG AGAAGAGGCC
    ATCAAGCCCT GTCGTCCTAT GACCAACAAT GCTGGCAGAC TTTTCCACTA CCGGATCACA
    GTCTCCCCGC CTACGAACTT TTTAACTGAC AGGCCAACTG TTATAGAATA CGATGATCAC
    GAGTATATCT TTGAAGGATT TTCTATGTTT GCACATGCCC CCCTGACCAA TATTCCACTG
    TGTAAAGTAA TTAGATTCAA CATAGACTAC ACGATTCATT TCATTGAAGA GATGATGCCG
    GAGAATTTTT GTGTGAAAGG GCTTGAACTC TTTTCACTGT TCCTATTCAG AGATATTTTG
    GAATTATATG ACTGGAATCT TAAAGGTCCT TTGTTTGAAG ACAGCCCTCC CTGCTGCCCA
    AGATTTCATT TCATGCCACG TTTTGTAAGA TTTCTTCCAG ATGGAGGAAA GGAAGTGCTG
    TCCATGCACC AGATTCTCCT GTACTTGTTA AGGTGCAGCA AAGCCCTGGT GCCTGAGGAG
    GAGATTGCCA ATATGCTTCA GTGGGAGGAG CTGGAGTGGC AGAAATATGC AGAAGAATGC
    AAAGGCATGA TTGTTACCAA CCCTGGGACG AAACCAAGCT CTGTCCGTAT CGATCAACTG
    GATCGTGAAC AGTTCAACCC CGATGTGATT ACTTTTCCGA TTATCGTCCA CTTTGGGATA
    CGCCCTGCAC AGTTGAGTTA TGCAGGAGAC CCACAGTACC AAAAACTGTG GAAGAGTTAT
    GTGAAACTTC GCCACCTCCT AGCAAATAGT CCCAAAGTCA AACAAACTGA CAAACAGAAG
    CTGGCACAGA GGGAGGAAGC CCTCCAAAAA ATACGGCAGA AGAATACAAT GAGACGAGAA
    GTAACGGTGG AGCTAAGTAG CCAAGGATTC TGGAAAACTG GCATCCGTTC TGATGTCTGT
    CAGCATGCAA TGATGCTACC TGTTCTGACC CATCATATCC GCTACCACCA ATGCCTAATG
    CATTTGGACA AGTTGATAGG ATATACTTTC CAAGATCGTT GTCTGTTGCA GCTGGCCATG
    ACTCATCCAA GTCATCATTT AAATTTTGGA ATGAATCCTG ATCATGCCAG GAATTCATTA
    TCTAACTGTG GAATTCGGCA GCCCAAATAC GGAGACAGAA AAGTTCATCA CATGCACATG
    CGGAAGAAAG GGATTAACAC CTTGATAAAT ATCATGTCAC GCCTTGGCCA AGATGACCCA
    ACTCCCTCGA GGATTAACCA CAATGAACGG TTGGAATTCC TGGGTGATGC TGTTGTTGAA
    TTTCTGACCA GCGTCCATTT GTACTATTTG TTTCCTAGTC TGGAAGAAGG AGGATTAGCA
    ACCTATCGGA CTGCCATTGT TCAGAATCAG CACCTTGCCA TGCTAGCAAA GAAACTTGAA
    CTGGATCGAT TTATGCTGTA TGCTCACGGG CCTGACCTTT GTAGAGAATC GGACCTTCGA
    CATGCAATGG CCAATTGTTT TGAAGCGTTA ATAGGAGCTG TTTACTTGGA GGGAAGCCTG
    GAGGAAGCCA AGCAGTTATT TGGACGCTTG CTCTTTAATG ATCCGGACCT GCGCGAAGTC
    TGGCTCAATT ATCCTCTCCA CCCACTCCAA CTACAAGAGC CAAATACTGA TCGACAACTT
    ATTGAAACTT CTCCAGTTCT ACAAAAACTT ACTGAGTTTG AAGAAGCAAT TGGAGTAATT
    TTTACTCATG TTCGACTTCT GGCAAGGGCA TTCACATTGA GAACTGTGGG ATTTAACCAT
    CTGACCCTAG GCCACAATCA GAGAATGGAA TTCCTAGGTG ACTCCATAAT GCAACTGGTA
    GCCACAGAGT ACTTATTCAT TCATTTCCCA GATCATCATG AAGGACACTT AACTTTGTTG
    CGAAGCTCTT TGGTGAATAA TAGAACTCAG GCCAAGGTAG CGGAGGAGCT GGGCATGCAG
    GAGTACGCCA TAACCAACGA CAAGACCAAG AGGCCTGTGG CGCTTCGCAC CAAGACCTTG
    GCGGACCTTT TGGAATCATT TATTGCAGCG CTGTACATTG ATAAGGATTT GGAATATGTT
    CATACTTTCA TGAATGTCTG CTTCTTTCCA CGATTGAAAG AGTTCATTTT GAATCAGGAT
    TGGAATGACC CCAAATCCCA GCTTCAGCAG TGTTGCTTGA CACTTAGGAC AGAAGGAAAA
    GAGCCAGACA TTCCTCTGTA CAAGACTCTG CAGACAGTGG GCCCATCCCA TGCCCGAACC
    TACACTGTGG CTGTTTATTT CAAGGGAGAA AGAATAGGCT GTGGGAAAGG ACCAAGTATT
    CAGCAAGCGG AAATGGGAGC AGCAATGGAT GCGCTTGAAA AATATAATTT TCCCCAGATG
    GCCCATCAGA AGCGGTTCAT CGAACGGAAG TACAGACAAG AGTTAAAAGA AATGAGGTGG
    GAAAGAGAGC ATCAAGAGAG AGAGCCAGAT GAGACTGAAG ACATCAAGAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024301803.1
    CDS289..4302
    Translation

    Target ORF information:

    RefSeq Version XM_024446035.1
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024446035.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGTCG CTCCCCAAGT
    AGGGAGAAGA AGAGAGCTCG TTGGGAGGAA GAAAAAGACC GTTGGAGTGA CAACCAGAGT
    TCTGGCAAAG ACAAGAACTA TACCTCAATC AAGGAAAAAG AGCCCGAGGA GACCATGCCT
    GACAAGAATG AGGAGGAAGA AGAAGAACTT CTTAAGCCTG TGTGGATTCG ATGCACTCAT
    TCAGAAAACT ACTACTCCAG TGACCCCATG GATCAGGTGG GAGATTCTAC AGTGGTTGGA
    ACGAGTAGGC TTCGTGACTT ATATGACAAA TTTGAGGAGG AGTTGGGGAG CAGGCAAGAA
    AAGGCCAAAG CTGCTCGGCC TCCGTGGGAA CCTCCAAAGA CGAAGCTCGA TGAAGATTTA
    GAGAGTTCCA GTGAATCCGA GTGTGAGTCT GATGAGGACA GCACCTGTTC TAGCAGCTCA
    GACTCTGAAG TTTTTGACGT TATTGCAGAA ATCAAACGCA AAAAGGCCCA CCCTGACCGA
    CTTCATGATG AACTTTGGTA CAACGATCCA GGCCAGATGA ATGATGGACC ACTCTGCAAA
    TGCAGCGCAA AGGCAAGACG CACAGGAATT AGGCACAGCA TTTATCCTGG AGAAGAGGCC
    ATCAAGCCCT GTCGTCCTAT GACCAACAAT GCTGGCAGAC TTTTCCACTA CCGGATCACA
    GTCTCCCCGC CTACGAACTT TTTAACTGAC AGGCCAACTG TTATAGAATA CGATGATCAC
    GAGTATATCT TTGAAGGATT TTCTATGTTT GCACATGCCC CCCTGACCAA TATTCCACTG
    TGTAAAGTAA TTAGATTCAA CATAGACTAC ACGATTCATT TCATTGAAGA GATGATGCCG
    GAGAATTTTT GTGTGAAAGG GCTTGAACTC TTTTCACTGT TCCTATTCAG AGATATTTTG
    GAATTATATG ACTGGAATCT TAAAGGTCCT TTGTTTGAAG ACAGCCCTCC CTGCTGCCCA
    AGATTTCATT TCATGCCACG TTTTGTAAGA TTTCTTCCAG ATGGAGGAAA GGAAGTGCTG
    TCCATGCACC AGATTCTCCT GTACTTGTTA AGGTGCAGCA AAGCCCTGGT GCCTGAGGAG
    GAGATTGCCA ATATGCTTCA GTGGGAGGAG CTGGAGTGGC AGAAATATGC AGAAGAATGC
    AAAGGCATGA TTGTTACCAA CCCTGGGACG AAACCAAGCT CTGTCCGTAT CGATCAACTG
    GATCGTGAAC AGTTCAACCC CGATGTGATT ACTTTTCCGA TTATCGTCCA CTTTGGGATA
    CGCCCTGCAC AGTTGAGTTA TGCAGGAGAC CCACAGTACC AAAAACTGTG GAAGAGTTAT
    GTGAAACTTC GCCACCTCCT AGCAAATAGT CCCAAAGTCA AACAAACTGA CAAACAGAAG
    CTGGCACAGA GGGAGGAAGC CCTCCAAAAA ATACGGCAGA AGAATACAAT GAGACGAGAA
    GTAACGGTGG AGCTAAGTAG CCAAGGATTC TGGAAAACTG GCATCCGTTC TGATGTCTGT
    CAGCATGCAA TGATGCTACC TGTTCTGACC CATCATATCC GCTACCACCA ATGCCTAATG
    CATTTGGACA AGTTGATAGG ATATACTTTC CAAGATCGTT GTCTGTTGCA GCTGGCCATG
    ACTCATCCAA GTCATCATTT AAATTTTGGA ATGAATCCTG ATCATGCCAG GAATTCATTA
    TCTAACTGTG GAATTCGGCA GCCCAAATAC GGAGACAGAA AAGTTCATCA CATGCACATG
    CGGAAGAAAG GGATTAACAC CTTGATAAAT ATCATGTCAC GCCTTGGCCA AGATGACCCA
    ACTCCCTCGA GGATTAACCA CAATGAACGG TTGGAATTCC TGGGTGATGC TGTTGTTGAA
    TTTCTGACCA GCGTCCATTT GTACTATTTG TTTCCTAGTC TGGAAGAAGG AGGATTAGCA
    ACCTATCGGA CTGCCATTGT TCAGAATCAG CACCTTGCCA TGCTAGCAAA GAAACTTGAA
    CTGGATCGAT TTATGCTGTA TGCTCACGGG CCTGACCTTT GTAGAGAATC GGACCTTCGA
    CATGCAATGG CCAATTGTTT TGAAGCGTTA ATAGGAGCTG TTTACTTGGA GGGAAGCCTG
    GAGGAAGCCA AGCAGTTATT TGGACGCTTG CTCTTTAATG ATCCGGACCT GCGCGAAGTC
    TGGCTCAATT ATCCTCTCCA CCCACTCCAA CTACAAGAGC CAAATACTGA TCGACAACTT
    ATTGAAACTT CTCCAGTTCT ACAAAAACTT ACTGAGTTTG AAGAAGCAAT TGGAGTAATT
    TTTACTCATG TTCGACTTCT GGCAAGGGCA TTCACATTGA GAACTGTGGG ATTTAACCAT
    CTGACCCTAG GCCACAATCA GAGAATGGAA TTCCTAGGTG ACTCCATAAT GCAACTGGTA
    GCCACAGAGT ACTTATTCAT TCATTTCCCA GATCATCATG AAGGACACTT AACTTTGTTG
    CGAAGCTCTT TGGTGAATAA TAGAACTCAG GCCAAGGTAG CGGAGGAGCT GGGCATGCAG
    GAGTACGCCA TAACCAACGA CAAGACCAAG AGGCCTGTGG CGCTTCGCAC CAAGACCTTG
    GCGGACCTTT TGGAATCATT TATTGCAGCG CTGTACATTG ATAAGGATTT GGAATATGTT
    CATACTTTCA TGAATGTCTG CTTCTTTCCA CGATTGAAAG AGTTCATTTT GAATCAGGAT
    TGGAATGACC CCAAATCCCA GCTTCAGCAG TGTTGCTTGA CACTTAGGAC AGAAGGAAAA
    GAGCCAGACA TTCCTCTGTA CAAGACTCTG CAGACAGTGG GCCCATCCCA TGCCCGAACC
    TACACTGTGG CTGTTTATTT CAAGGGAGAA AGAATAGGCT GTGGGAAAGG ACCAAGTATT
    CAGCAAGCGG AAATGGGAGC AGCAATGGAT GCGCTTGAAA AATATAATTT TCCCCAGATG
    GCCCATCAGA AGCGGTTCAT CGAACGGAAG TACAGACAAG AGTTAAAAGA AATGAGGTGG
    GAAAGAGAGC ATCAAGAGAG AGAGCCAGAT GAGACTGAAG ACATCAAGAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16612
    Clone ID Related Accession (Same CDS sequence) XM_005248291.3 , NM_013235.5 , XM_005248291.4 , XM_024446034.1 , NM_013235.4 , XM_024446033.1
    Accession Version XM_005248291.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248291.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATCAT TTATTGCAGC GCTGTACATT GATAAGGATT TGGAATATGT TCATACTTTC
    ATGAATGTCT GCTTCTTTCC ACGATTGAAA GAGTTCATTT TGAATCAGGA TTGGAATGAC
    CCCAAATCCC AGCTTCAGCA GTGTTGCTTG ACACTTAGGA CAGAAGGAAA AGAGCCAGAC
    ATTCCTCTGT ACAAGACTCT GCAGACAGTG GGCCCATCCC ATGCCCGAAC CTACACTGTG
    GCTGTTTATT TCAAGGGAGA AAGAATAGGC TGTGGGAAAG GACCAAGTAT TCAGCAAGCG
    GAAATGGGAG CAGCAATGGA TGCGCTTGAA AAATATAATT TTCCCCAGAT GGCCCATCAG
    AAGCGGTTCA TCGAACGGAA GTACAGACAA GAGTTAAAAG AAATGAGGTG GGAAAGAGAG
    CATCAAGAGA GAGAGCCAGA TGAGACTGAA GACATCAAGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248348.1
    CDS436..4560
    Translation

    Target ORF information:

    RefSeq Version XM_005248291.3
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248291.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATCAT TTATTGCAGC GCTGTACATT GATAAGGATT TGGAATATGT TCATACTTTC
    ATGAATGTCT GCTTCTTTCC ACGATTGAAA GAGTTCATTT TGAATCAGGA TTGGAATGAC
    CCCAAATCCC AGCTTCAGCA GTGTTGCTTG ACACTTAGGA CAGAAGGAAA AGAGCCAGAC
    ATTCCTCTGT ACAAGACTCT GCAGACAGTG GGCCCATCCC ATGCCCGAAC CTACACTGTG
    GCTGTTTATT TCAAGGGAGA AAGAATAGGC TGTGGGAAAG GACCAAGTAT TCAGCAAGCG
    GAAATGGGAG CAGCAATGGA TGCGCTTGAA AAATATAATT TTCCCCAGAT GGCCCATCAG
    AAGCGGTTCA TCGAACGGAA GTACAGACAA GAGTTAAAAG AAATGAGGTG GGAAAGAGAG
    CATCAAGAGA GAGAGCCAGA TGAGACTGAA GACATCAAGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16612
    Clone ID Related Accession (Same CDS sequence) XM_005248291.3 , NM_013235.5 , XM_005248291.4 , XM_024446034.1 , NM_013235.4 , XM_024446033.1
    Accession Version XM_005248291.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248291.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATCAT TTATTGCAGC GCTGTACATT GATAAGGATT TGGAATATGT TCATACTTTC
    ATGAATGTCT GCTTCTTTCC ACGATTGAAA GAGTTCATTT TGAATCAGGA TTGGAATGAC
    CCCAAATCCC AGCTTCAGCA GTGTTGCTTG ACACTTAGGA CAGAAGGAAA AGAGCCAGAC
    ATTCCTCTGT ACAAGACTCT GCAGACAGTG GGCCCATCCC ATGCCCGAAC CTACACTGTG
    GCTGTTTATT TCAAGGGAGA AAGAATAGGC TGTGGGAAAG GACCAAGTAT TCAGCAAGCG
    GAAATGGGAG CAGCAATGGA TGCGCTTGAA AAATATAATT TTCCCCAGAT GGCCCATCAG
    AAGCGGTTCA TCGAACGGAA GTACAGACAA GAGTTAAAAG AAATGAGGTG GGAAAGAGAG
    CATCAAGAGA GAGAGCCAGA TGAGACTGAA GACATCAAGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248348.1
    CDS403..4527
    Translation

    Target ORF information:

    RefSeq Version XM_005248291.4
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248291.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATCAT TTATTGCAGC GCTGTACATT GATAAGGATT TGGAATATGT TCATACTTTC
    ATGAATGTCT GCTTCTTTCC ACGATTGAAA GAGTTCATTT TGAATCAGGA TTGGAATGAC
    CCCAAATCCC AGCTTCAGCA GTGTTGCTTG ACACTTAGGA CAGAAGGAAA AGAGCCAGAC
    ATTCCTCTGT ACAAGACTCT GCAGACAGTG GGCCCATCCC ATGCCCGAAC CTACACTGTG
    GCTGTTTATT TCAAGGGAGA AAGAATAGGC TGTGGGAAAG GACCAAGTAT TCAGCAAGCG
    GAAATGGGAG CAGCAATGGA TGCGCTTGAA AAATATAATT TTCCCCAGAT GGCCCATCAG
    AAGCGGTTCA TCGAACGGAA GTACAGACAA GAGTTAAAAG AAATGAGGTG GGAAAGAGAG
    CATCAAGAGA GAGAGCCAGA TGAGACTGAA GACATCAAGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16612
    Clone ID Related Accession (Same CDS sequence) XM_005248291.3 , NM_013235.5 , XM_005248291.4 , XM_024446034.1 , NM_013235.4 , XM_024446033.1
    Accession Version XM_024446034.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATCAT TTATTGCAGC GCTGTACATT GATAAGGATT TGGAATATGT TCATACTTTC
    ATGAATGTCT GCTTCTTTCC ACGATTGAAA GAGTTCATTT TGAATCAGGA TTGGAATGAC
    CCCAAATCCC AGCTTCAGCA GTGTTGCTTG ACACTTAGGA CAGAAGGAAA AGAGCCAGAC
    ATTCCTCTGT ACAAGACTCT GCAGACAGTG GGCCCATCCC ATGCCCGAAC CTACACTGTG
    GCTGTTTATT TCAAGGGAGA AAGAATAGGC TGTGGGAAAG GACCAAGTAT TCAGCAAGCG
    GAAATGGGAG CAGCAATGGA TGCGCTTGAA AAATATAATT TTCCCCAGAT GGCCCATCAG
    AAGCGGTTCA TCGAACGGAA GTACAGACAA GAGTTAAAAG AAATGAGGTG GGAAAGAGAG
    CATCAAGAGA GAGAGCCAGA TGAGACTGAA GACATCAAGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024301802.1
    CDS265..4389
    Translation

    Target ORF information:

    RefSeq Version XM_024446034.1
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024446034.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATCAT TTATTGCAGC GCTGTACATT GATAAGGATT TGGAATATGT TCATACTTTC
    ATGAATGTCT GCTTCTTTCC ACGATTGAAA GAGTTCATTT TGAATCAGGA TTGGAATGAC
    CCCAAATCCC AGCTTCAGCA GTGTTGCTTG ACACTTAGGA CAGAAGGAAA AGAGCCAGAC
    ATTCCTCTGT ACAAGACTCT GCAGACAGTG GGCCCATCCC ATGCCCGAAC CTACACTGTG
    GCTGTTTATT TCAAGGGAGA AAGAATAGGC TGTGGGAAAG GACCAAGTAT TCAGCAAGCG
    GAAATGGGAG CAGCAATGGA TGCGCTTGAA AAATATAATT TTCCCCAGAT GGCCCATCAG
    AAGCGGTTCA TCGAACGGAA GTACAGACAA GAGTTAAAAG AAATGAGGTG GGAAAGAGAG
    CATCAAGAGA GAGAGCCAGA TGAGACTGAA GACATCAAGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16612
    Clone ID Related Accession (Same CDS sequence) XM_005248291.3 , NM_013235.5 , XM_005248291.4 , XM_024446034.1 , NM_013235.4 , XM_024446033.1
    Accession Version XM_024446033.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATCAT TTATTGCAGC GCTGTACATT GATAAGGATT TGGAATATGT TCATACTTTC
    ATGAATGTCT GCTTCTTTCC ACGATTGAAA GAGTTCATTT TGAATCAGGA TTGGAATGAC
    CCCAAATCCC AGCTTCAGCA GTGTTGCTTG ACACTTAGGA CAGAAGGAAA AGAGCCAGAC
    ATTCCTCTGT ACAAGACTCT GCAGACAGTG GGCCCATCCC ATGCCCGAAC CTACACTGTG
    GCTGTTTATT TCAAGGGAGA AAGAATAGGC TGTGGGAAAG GACCAAGTAT TCAGCAAGCG
    GAAATGGGAG CAGCAATGGA TGCGCTTGAA AAATATAATT TTCCCCAGAT GGCCCATCAG
    AAGCGGTTCA TCGAACGGAA GTACAGACAA GAGTTAAAAG AAATGAGGTG GGAAAGAGAG
    CATCAAGAGA GAGAGCCAGA TGAGACTGAA GACATCAAGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024301801.1
    CDS348..4472
    Translation

    Target ORF information:

    RefSeq Version XM_024446033.1
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024446033.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTTGAGTT ATGCAGGAGA CCCACAGTAC CAAAAACTGT GGAAGAGTTA TGTGAAACTT
    CGCCACCTCC TAGCAAATAG TCCCAAAGTC AAACAAACTG ACAAACAGAA GCTGGCACAG
    AGGGAGGAAG CCCTCCAAAA AATACGGCAG AAGAATACAA TGAGACGAGA AGTAACGGTG
    GAGCTAAGTA GCCAAGGATT CTGGAAAACT GGCATCCGTT CTGATGTCTG TCAGCATGCA
    ATGATGCTAC CTGTTCTGAC CCATCATATC CGCTACCACC AATGCCTAAT GCATTTGGAC
    AAGTTGATAG GATATACTTT CCAAGATCGT TGTCTGTTGC AGCTGGCCAT GACTCATCCA
    AGTCATCATT TAAATTTTGG AATGAATCCT GATCATGCCA GGAATTCATT ATCTAACTGT
    GGAATTCGGC AGCCCAAATA CGGAGACAGA AAAGTTCATC ACATGCACAT GCGGAAGAAA
    GGGATTAACA CCTTGATAAA TATCATGTCA CGCCTTGGCC AAGATGACCC AACTCCCTCG
    AGGATTAACC ACAATGAACG GTTGGAATTC CTGGGTGATG CTGTTGTTGA ATTTCTGACC
    AGCGTCCATT TGTACTATTT GTTTCCTAGT CTGGAAGAAG GAGGATTAGC AACCTATCGG
    ACTGCCATTG TTCAGAATCA GCACCTTGCC ATGCTAGCAA AGAAACTTGA ACTGGATCGA
    TTTATGCTGT ATGCTCACGG GCCTGACCTT TGTAGAGAAT CGGACCTTCG ACATGCAATG
    GCCAATTGTT TTGAAGCGTT AATAGGAGCT GTTTACTTGG AGGGAAGCCT GGAGGAAGCC
    AAGCAGTTAT TTGGACGCTT GCTCTTTAAT GATCCGGACC TGCGCGAAGT CTGGCTCAAT
    TATCCTCTCC ACCCACTCCA ACTACAAGAG CCAAATACTG ATCGACAACT TATTGAAACT
    TCTCCAGTTC TACAAAAACT TACTGAGTTT GAAGAAGCAA TTGGAGTAAT TTTTACTCAT
    GTTCGACTTC TGGCAAGGGC ATTCACATTG AGAACTGTGG GATTTAACCA TCTGACCCTA
    GGCCACAATC AGAGAATGGA ATTCCTAGGT GACTCCATAA TGCAACTGGT AGCCACAGAG
    TACTTATTCA TTCATTTCCC AGATCATCAT GAAGGACACT TAACTTTGTT GCGAAGCTCT
    TTGGTGAATA ATAGAACTCA GGCCAAGGTA GCGGAGGAGC TGGGCATGCA GGAGTACGCC
    ATAACCAACG ACAAGACCAA GAGGCCTGTG GCGCTTCGCA CCAAGACCTT GGCGGACCTT
    TTGGAATCAT TTATTGCAGC GCTGTACATT GATAAGGATT TGGAATATGT TCATACTTTC
    ATGAATGTCT GCTTCTTTCC ACGATTGAAA GAGTTCATTT TGAATCAGGA TTGGAATGAC
    CCCAAATCCC AGCTTCAGCA GTGTTGCTTG ACACTTAGGA CAGAAGGAAA AGAGCCAGAC
    ATTCCTCTGT ACAAGACTCT GCAGACAGTG GGCCCATCCC ATGCCCGAAC CTACACTGTG
    GCTGTTTATT TCAAGGGAGA AAGAATAGGC TGTGGGAAAG GACCAAGTAT TCAGCAAGCG
    GAAATGGGAG CAGCAATGGA TGCGCTTGAA AAATATAATT TTCCCCAGAT GGCCCATCAG
    AAGCGGTTCA TCGAACGGAA GTACAGACAA GAGTTAAAAG AAATGAGGTG GGAAAGAGAG
    CATCAAGAGA GAGAGCCAGA TGAGACTGAA GACATCAAGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39314
    Clone ID Related Accession (Same CDS sequence) XM_005248292.4 , XM_005248292.3
    Accession Version XM_005248292.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248292.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTACCAAA AACTGTGGAA GAGTTATGTG AAACTTCGCC ACCTCCTAGC AAATAGTCCC
    AAAGTCAAAC AAACTGACAA ACAGAAGCTG GCACAGAGGG AGGAAGCCCT CCAAAAAATA
    CGGCAGAAGA ATACAATGAG ACGAGAAGTA ACGGTGGAGC TAAGTAGCCA AGGATTCTGG
    AAAACTGGCA TCCGTTCTGA TGTCTGTCAG CATGCAATGA TGCTACCTGT TCTGACCCAT
    CATATCCGCT ACCACCAATG CCTAATGCAT TTGGACAAGT TGATAGGATA TACTTTCCAA
    GATCGTTGTC TGTTGCAGCT GGCCATGACT CATCCAAGTC ATCATTTAAA TTTTGGAATG
    AATCCTGATC ATGCCAGGAA TTCATTATCT AACTGTGGAA TTCGGCAGCC CAAATACGGA
    GACAGAAAAG TTCATCACAT GCACATGCGG AAGAAAGGGA TTAACACCTT GATAAATATC
    ATGTCACGCC TTGGCCAAGA TGACCCAACT CCCTCGAGGA TTAACCACAA TGAACGGTTG
    GAATTCCTGG GTGATGCTGT TGTTGAATTT CTGACCAGCG TCCATTTGTA CTATTTGTTT
    CCTAGTCTGG AAGAAGGAGG ATTAGCAACC TATCGGACTG CCATTGTTCA GAATCAGCAC
    CTTGCCATGC TAGCAAAGAA ACTTGAACTG GATCGATTTA TGCTGTATGC TCACGGGCCT
    GACCTTTGTA GAGAATCGGA CCTTCGACAT GCAATGGCCA ATTGTTTTGA AGCGTTAATA
    GGAGCTGTTT ACTTGGAGGG AAGCCTGGAG GAAGCCAAGC AGTTATTTGG ACGCTTGCTC
    TTTAATGATC CGGACCTGCG CGAAGTCTGG CTCAATTATC CTCTCCACCC ACTCCAACTA
    CAAGAGCCAA ATACTGATCG ACAACTTATT GAAACTTCTC CAGTTCTACA AAAACTTACT
    GAGTTTGAAG AAGCAATTGG AGTAATTTTT ACTCATGTTC GACTTCTGGC AAGGGCATTC
    ACATTGAGAA CTGTGGGATT TAACCATCTG ACCCTAGGCC ACAATCAGAG AATGGAATTC
    CTAGGTGACT CCATAATGCA ACTGGTAGCC ACAGAGTACT TATTCATTCA TTTCCCAGAT
    CATCATGAAG GACACTTAAC TTTGTTGCGA AGCTCTTTGG TGAATAATAG AACTCAGGCC
    AAGGTAGCGG AGGAGCTGGG CATGCAGGAG TACGCCATAA CCAACGACAA GACCAAGAGG
    CCTGTGGCGC TTCGCACCAA GACCTTGGCG GACCTTTTGG AATCATTTAT TGCAGCGCTG
    TACATTGATA AGGATTTGGA ATATGTTCAT ACTTTCATGA ATGTCTGCTT CTTTCCACGA
    TTGAAAGAGT TCATTTTGAA TCAGGATTGG AATGACCCCA AATCCCAGCT TCAGCAGTGT
    TGCTTGACAC TTAGGACAGA AGGAAAAGAG CCAGACATTC CTCTGTACAA GACTCTGCAG
    ACAGTGGGCC CATCCCATGC CCGAACCTAC ACTGTGGCTG TTTATTTCAA GGGAGAAAGA
    ATAGGCTGTG GGAAAGGACC AAGTATTCAG CAAGCGGAAA TGGGAGCAGC AATGGATGCG
    CTTGAAAAAT ATAATTTTCC CCAGATGGCC CATCAGAAGC GGTTCATCGA ACGGAAGTAC
    AGACAAGAGT TAAAAGAAAT GAGGTGGGAA AGAGAGCATC AAGAGAGAGA GCCAGATGAG
    ACTGAAGACA TCAAGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248349.1
    CDS403..4503
    Translation

    Target ORF information:

    RefSeq Version XM_005248292.4
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248292.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTACCAAA AACTGTGGAA GAGTTATGTG AAACTTCGCC ACCTCCTAGC AAATAGTCCC
    AAAGTCAAAC AAACTGACAA ACAGAAGCTG GCACAGAGGG AGGAAGCCCT CCAAAAAATA
    CGGCAGAAGA ATACAATGAG ACGAGAAGTA ACGGTGGAGC TAAGTAGCCA AGGATTCTGG
    AAAACTGGCA TCCGTTCTGA TGTCTGTCAG CATGCAATGA TGCTACCTGT TCTGACCCAT
    CATATCCGCT ACCACCAATG CCTAATGCAT TTGGACAAGT TGATAGGATA TACTTTCCAA
    GATCGTTGTC TGTTGCAGCT GGCCATGACT CATCCAAGTC ATCATTTAAA TTTTGGAATG
    AATCCTGATC ATGCCAGGAA TTCATTATCT AACTGTGGAA TTCGGCAGCC CAAATACGGA
    GACAGAAAAG TTCATCACAT GCACATGCGG AAGAAAGGGA TTAACACCTT GATAAATATC
    ATGTCACGCC TTGGCCAAGA TGACCCAACT CCCTCGAGGA TTAACCACAA TGAACGGTTG
    GAATTCCTGG GTGATGCTGT TGTTGAATTT CTGACCAGCG TCCATTTGTA CTATTTGTTT
    CCTAGTCTGG AAGAAGGAGG ATTAGCAACC TATCGGACTG CCATTGTTCA GAATCAGCAC
    CTTGCCATGC TAGCAAAGAA ACTTGAACTG GATCGATTTA TGCTGTATGC TCACGGGCCT
    GACCTTTGTA GAGAATCGGA CCTTCGACAT GCAATGGCCA ATTGTTTTGA AGCGTTAATA
    GGAGCTGTTT ACTTGGAGGG AAGCCTGGAG GAAGCCAAGC AGTTATTTGG ACGCTTGCTC
    TTTAATGATC CGGACCTGCG CGAAGTCTGG CTCAATTATC CTCTCCACCC ACTCCAACTA
    CAAGAGCCAA ATACTGATCG ACAACTTATT GAAACTTCTC CAGTTCTACA AAAACTTACT
    GAGTTTGAAG AAGCAATTGG AGTAATTTTT ACTCATGTTC GACTTCTGGC AAGGGCATTC
    ACATTGAGAA CTGTGGGATT TAACCATCTG ACCCTAGGCC ACAATCAGAG AATGGAATTC
    CTAGGTGACT CCATAATGCA ACTGGTAGCC ACAGAGTACT TATTCATTCA TTTCCCAGAT
    CATCATGAAG GACACTTAAC TTTGTTGCGA AGCTCTTTGG TGAATAATAG AACTCAGGCC
    AAGGTAGCGG AGGAGCTGGG CATGCAGGAG TACGCCATAA CCAACGACAA GACCAAGAGG
    CCTGTGGCGC TTCGCACCAA GACCTTGGCG GACCTTTTGG AATCATTTAT TGCAGCGCTG
    TACATTGATA AGGATTTGGA ATATGTTCAT ACTTTCATGA ATGTCTGCTT CTTTCCACGA
    TTGAAAGAGT TCATTTTGAA TCAGGATTGG AATGACCCCA AATCCCAGCT TCAGCAGTGT
    TGCTTGACAC TTAGGACAGA AGGAAAAGAG CCAGACATTC CTCTGTACAA GACTCTGCAG
    ACAGTGGGCC CATCCCATGC CCGAACCTAC ACTGTGGCTG TTTATTTCAA GGGAGAAAGA
    ATAGGCTGTG GGAAAGGACC AAGTATTCAG CAAGCGGAAA TGGGAGCAGC AATGGATGCG
    CTTGAAAAAT ATAATTTTCC CCAGATGGCC CATCAGAAGC GGTTCATCGA ACGGAAGTAC
    AGACAAGAGT TAAAAGAAAT GAGGTGGGAA AGAGAGCATC AAGAGAGAGA GCCAGATGAG
    ACTGAAGACA TCAAGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39314
    Clone ID Related Accession (Same CDS sequence) XM_005248292.4 , XM_005248292.3
    Accession Version XM_005248292.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248292.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTACCAAA AACTGTGGAA GAGTTATGTG AAACTTCGCC ACCTCCTAGC AAATAGTCCC
    AAAGTCAAAC AAACTGACAA ACAGAAGCTG GCACAGAGGG AGGAAGCCCT CCAAAAAATA
    CGGCAGAAGA ATACAATGAG ACGAGAAGTA ACGGTGGAGC TAAGTAGCCA AGGATTCTGG
    AAAACTGGCA TCCGTTCTGA TGTCTGTCAG CATGCAATGA TGCTACCTGT TCTGACCCAT
    CATATCCGCT ACCACCAATG CCTAATGCAT TTGGACAAGT TGATAGGATA TACTTTCCAA
    GATCGTTGTC TGTTGCAGCT GGCCATGACT CATCCAAGTC ATCATTTAAA TTTTGGAATG
    AATCCTGATC ATGCCAGGAA TTCATTATCT AACTGTGGAA TTCGGCAGCC CAAATACGGA
    GACAGAAAAG TTCATCACAT GCACATGCGG AAGAAAGGGA TTAACACCTT GATAAATATC
    ATGTCACGCC TTGGCCAAGA TGACCCAACT CCCTCGAGGA TTAACCACAA TGAACGGTTG
    GAATTCCTGG GTGATGCTGT TGTTGAATTT CTGACCAGCG TCCATTTGTA CTATTTGTTT
    CCTAGTCTGG AAGAAGGAGG ATTAGCAACC TATCGGACTG CCATTGTTCA GAATCAGCAC
    CTTGCCATGC TAGCAAAGAA ACTTGAACTG GATCGATTTA TGCTGTATGC TCACGGGCCT
    GACCTTTGTA GAGAATCGGA CCTTCGACAT GCAATGGCCA ATTGTTTTGA AGCGTTAATA
    GGAGCTGTTT ACTTGGAGGG AAGCCTGGAG GAAGCCAAGC AGTTATTTGG ACGCTTGCTC
    TTTAATGATC CGGACCTGCG CGAAGTCTGG CTCAATTATC CTCTCCACCC ACTCCAACTA
    CAAGAGCCAA ATACTGATCG ACAACTTATT GAAACTTCTC CAGTTCTACA AAAACTTACT
    GAGTTTGAAG AAGCAATTGG AGTAATTTTT ACTCATGTTC GACTTCTGGC AAGGGCATTC
    ACATTGAGAA CTGTGGGATT TAACCATCTG ACCCTAGGCC ACAATCAGAG AATGGAATTC
    CTAGGTGACT CCATAATGCA ACTGGTAGCC ACAGAGTACT TATTCATTCA TTTCCCAGAT
    CATCATGAAG GACACTTAAC TTTGTTGCGA AGCTCTTTGG TGAATAATAG AACTCAGGCC
    AAGGTAGCGG AGGAGCTGGG CATGCAGGAG TACGCCATAA CCAACGACAA GACCAAGAGG
    CCTGTGGCGC TTCGCACCAA GACCTTGGCG GACCTTTTGG AATCATTTAT TGCAGCGCTG
    TACATTGATA AGGATTTGGA ATATGTTCAT ACTTTCATGA ATGTCTGCTT CTTTCCACGA
    TTGAAAGAGT TCATTTTGAA TCAGGATTGG AATGACCCCA AATCCCAGCT TCAGCAGTGT
    TGCTTGACAC TTAGGACAGA AGGAAAAGAG CCAGACATTC CTCTGTACAA GACTCTGCAG
    ACAGTGGGCC CATCCCATGC CCGAACCTAC ACTGTGGCTG TTTATTTCAA GGGAGAAAGA
    ATAGGCTGTG GGAAAGGACC AAGTATTCAG CAAGCGGAAA TGGGAGCAGC AATGGATGCG
    CTTGAAAAAT ATAATTTTCC CCAGATGGCC CATCAGAAGC GGTTCATCGA ACGGAAGTAC
    AGACAAGAGT TAAAAGAAAT GAGGTGGGAA AGAGAGCATC AAGAGAGAGA GCCAGATGAG
    ACTGAAGACA TCAAGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248349.1
    CDS436..4536
    Translation

    Target ORF information:

    RefSeq Version XM_005248292.3
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248292.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGAGAGCG AGAACGGGAG AGACACAGGC ATCGAGACAA CCGAAGATCA
    CCATCTCTGG AAAGGTCCTA CAAAAAAGAG TATAAGAGAT CTGGAAGGAG TTACGGTTTA
    TCGGTTGTTC CTGAACCTGC TGGATGCACA CCAGAATTAC CTGGGGAGAT TATTAAAAAT
    ACAGATTCTT GGGCCCCACC CCTGGAGATT GTGAATCATC GCTCCCCAAG TAGGGAGAAG
    AAGAGAGCTC GTTGGGAGGA AGAAAAAGAC CGTTGGAGTG ACAACCAGAG TTCTGGCAAA
    GACAAGAACT ATACCTCAAT CAAGGAAAAA GAGCCCGAGG AGACCATGCC TGACAAGAAT
    GAGGAGGAAG AAGAAGAACT TCTTAAGCCT GTGTGGATTC GATGCACTCA TTCAGAAAAC
    TACTACTCCA GTGACCCCAT GGATCAGGTG GGAGATTCTA CAGTGGTTGG AACGAGTAGG
    CTTCGTGACT TATATGACAA ATTTGAGGAG GAGTTGGGGA GCAGGCAAGA AAAGGCCAAA
    GCTGCTCGGC CTCCGTGGGA ACCTCCAAAG ACGAAGCTCG ATGAAGATTT AGAGAGTTCC
    AGTGAATCCG AGTGTGAGTC TGATGAGGAC AGCACCTGTT CTAGCAGCTC AGACTCTGAA
    GTTTTTGACG TTATTGCAGA AATCAAACGC AAAAAGGCCC ACCCTGACCG ACTTCATGAT
    GAACTTTGGT ACAACGATCC AGGCCAGATG AATGATGGAC CACTCTGCAA ATGCAGCGCA
    AAGGCAAGAC GCACAGGAAT TAGGCACAGC ATTTATCCTG GAGAAGAGGC CATCAAGCCC
    TGTCGTCCTA TGACCAACAA TGCTGGCAGA CTTTTCCACT ACCGGATCAC AGTCTCCCCG
    CCTACGAACT TTTTAACTGA CAGGCCAACT GTTATAGAAT ACGATGATCA CGAGTATATC
    TTTGAAGGAT TTTCTATGTT TGCACATGCC CCCCTGACCA ATATTCCACT GTGTAAAGTA
    ATTAGATTCA ACATAGACTA CACGATTCAT TTCATTGAAG AGATGATGCC GGAGAATTTT
    TGTGTGAAAG GGCTTGAACT CTTTTCACTG TTCCTATTCA GAGATATTTT GGAATTATAT
    GACTGGAATC TTAAAGGTCC TTTGTTTGAA GACAGCCCTC CCTGCTGCCC AAGATTTCAT
    TTCATGCCAC GTTTTGTAAG ATTTCTTCCA GATGGAGGAA AGGAAGTGCT GTCCATGCAC
    CAGATTCTCC TGTACTTGTT AAGGTGCAGC AAAGCCCTGG TGCCTGAGGA GGAGATTGCC
    AATATGCTTC AGTGGGAGGA GCTGGAGTGG CAGAAATATG CAGAAGAATG CAAAGGCATG
    ATTGTTACCA ACCCTGGGAC GAAACCAAGC TCTGTCCGTA TCGATCAACT GGATCGTGAA
    CAGTTCAACC CCGATGTGAT TACTTTTCCG ATTATCGTCC ACTTTGGGAT ACGCCCTGCA
    CAGTACCAAA AACTGTGGAA GAGTTATGTG AAACTTCGCC ACCTCCTAGC AAATAGTCCC
    AAAGTCAAAC AAACTGACAA ACAGAAGCTG GCACAGAGGG AGGAAGCCCT CCAAAAAATA
    CGGCAGAAGA ATACAATGAG ACGAGAAGTA ACGGTGGAGC TAAGTAGCCA AGGATTCTGG
    AAAACTGGCA TCCGTTCTGA TGTCTGTCAG CATGCAATGA TGCTACCTGT TCTGACCCAT
    CATATCCGCT ACCACCAATG CCTAATGCAT TTGGACAAGT TGATAGGATA TACTTTCCAA
    GATCGTTGTC TGTTGCAGCT GGCCATGACT CATCCAAGTC ATCATTTAAA TTTTGGAATG
    AATCCTGATC ATGCCAGGAA TTCATTATCT AACTGTGGAA TTCGGCAGCC CAAATACGGA
    GACAGAAAAG TTCATCACAT GCACATGCGG AAGAAAGGGA TTAACACCTT GATAAATATC
    ATGTCACGCC TTGGCCAAGA TGACCCAACT CCCTCGAGGA TTAACCACAA TGAACGGTTG
    GAATTCCTGG GTGATGCTGT TGTTGAATTT CTGACCAGCG TCCATTTGTA CTATTTGTTT
    CCTAGTCTGG AAGAAGGAGG ATTAGCAACC TATCGGACTG CCATTGTTCA GAATCAGCAC
    CTTGCCATGC TAGCAAAGAA ACTTGAACTG GATCGATTTA TGCTGTATGC TCACGGGCCT
    GACCTTTGTA GAGAATCGGA CCTTCGACAT GCAATGGCCA ATTGTTTTGA AGCGTTAATA
    GGAGCTGTTT ACTTGGAGGG AAGCCTGGAG GAAGCCAAGC AGTTATTTGG ACGCTTGCTC
    TTTAATGATC CGGACCTGCG CGAAGTCTGG CTCAATTATC CTCTCCACCC ACTCCAACTA
    CAAGAGCCAA ATACTGATCG ACAACTTATT GAAACTTCTC CAGTTCTACA AAAACTTACT
    GAGTTTGAAG AAGCAATTGG AGTAATTTTT ACTCATGTTC GACTTCTGGC AAGGGCATTC
    ACATTGAGAA CTGTGGGATT TAACCATCTG ACCCTAGGCC ACAATCAGAG AATGGAATTC
    CTAGGTGACT CCATAATGCA ACTGGTAGCC ACAGAGTACT TATTCATTCA TTTCCCAGAT
    CATCATGAAG GACACTTAAC TTTGTTGCGA AGCTCTTTGG TGAATAATAG AACTCAGGCC
    AAGGTAGCGG AGGAGCTGGG CATGCAGGAG TACGCCATAA CCAACGACAA GACCAAGAGG
    CCTGTGGCGC TTCGCACCAA GACCTTGGCG GACCTTTTGG AATCATTTAT TGCAGCGCTG
    TACATTGATA AGGATTTGGA ATATGTTCAT ACTTTCATGA ATGTCTGCTT CTTTCCACGA
    TTGAAAGAGT TCATTTTGAA TCAGGATTGG AATGACCCCA AATCCCAGCT TCAGCAGTGT
    TGCTTGACAC TTAGGACAGA AGGAAAAGAG CCAGACATTC CTCTGTACAA GACTCTGCAG
    ACAGTGGGCC CATCCCATGC CCGAACCTAC ACTGTGGCTG TTTATTTCAA GGGAGAAAGA
    ATAGGCTGTG GGAAAGGACC AAGTATTCAG CAAGCGGAAA TGGGAGCAGC AATGGATGCG
    CTTGAAAAAT ATAATTTTCC CCAGATGGCC CATCAGAAGC GGTTCATCGA ACGGAAGTAC
    AGACAAGAGT TAAAAGAAAT GAGGTGGGAA AGAGAGCATC AAGAGAGAGA GCCAGATGAG
    ACTGAAGACA TCAAGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39315
    Clone ID Related Accession (Same CDS sequence) XM_005248293.3 , XM_005248293.4
    Accession Version XM_005248293.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248293.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGGAGTTA CGGTTTATCG GTTGTTCCTG AACCTGCTGG ATGCACACCA
    GAATTACCTG GGGAGATTAT TAAAAATACA GATTCTTGGG CCCCACCCCT GGAGATTGTG
    AATCATCGCT CCCCAAGTAG GGAGAAGAAG AGAGCTCGTT GGGAGGAAGA AAAAGACCGT
    TGGAGTGACA ACCAGAGTTC TGGCAAAGAC AAGAACTATA CCTCAATCAA GGAAAAAGAG
    CCCGAGGAGA CCATGCCTGA CAAGAATGAG GAGGAAGAAG AAGAACTTCT TAAGCCTGTG
    TGGATTCGAT GCACTCATTC AGAAAACTAC TACTCCAGTG ACCCCATGGA TCAGGTGGGA
    GATTCTACAG TGGTTGGAAC GAGTAGGCTT CGTGACTTAT ATGACAAATT TGAGGAGGAG
    TTGGGGAGCA GGCAAGAAAA GGCCAAAGCT GCTCGGCCTC CGTGGGAACC TCCAAAGACG
    AAGCTCGATG AAGATTTAGA GAGTTCCAGT GAATCCGAGT GTGAGTCTGA TGAGGACAGC
    ACCTGTTCTA GCAGCTCAGA CTCTGAAGTT TTTGACGTTA TTGCAGAAAT CAAACGCAAA
    AAGGCCCACC CTGACCGACT TCATGATGAA CTTTGGTACA ACGATCCAGG CCAGATGAAT
    GATGGACCAC TCTGCAAATG CAGCGCAAAG GCAAGACGCA CAGGAATTAG GCACAGCATT
    TATCCTGGAG AAGAGGCCAT CAAGCCCTGT CGTCCTATGA CCAACAATGC TGGCAGACTT
    TTCCACTACC GGATCACAGT CTCCCCGCCT ACGAACTTTT TAACTGACAG GCCAACTGTT
    ATAGAATACG ATGATCACGA GTATATCTTT GAAGGATTTT CTATGTTTGC ACATGCCCCC
    CTGACCAATA TTCCACTGTG TAAAGTAATT AGATTCAACA TAGACTACAC GATTCATTTC
    ATTGAAGAGA TGATGCCGGA GAATTTTTGT GTGAAAGGGC TTGAACTCTT TTCACTGTTC
    CTATTCAGAG ATATTTTGGA ATTATATGAC TGGAATCTTA AAGGTCCTTT GTTTGAAGAC
    AGCCCTCCCT GCTGCCCAAG ATTTCATTTC ATGCCACGTT TTGTAAGATT TCTTCCAGAT
    GGAGGAAAGG AAGTGCTGTC CATGCACCAG ATTCTCCTGT ACTTGTTAAG GTGCAGCAAA
    GCCCTGGTGC CTGAGGAGGA GATTGCCAAT ATGCTTCAGT GGGAGGAGCT GGAGTGGCAG
    AAATATGCAG AAGAATGCAA AGGCATGATT GTTACCAACC CTGGGACGAA ACCAAGCTCT
    GTCCGTATCG ATCAACTGGA TCGTGAACAG TTCAACCCCG ATGTGATTAC TTTTCCGATT
    ATCGTCCACT TTGGGATACG CCCTGCACAG TTGAGTTATG CAGGAGACCC ACAGTACCAA
    AAACTGTGGA AGAGTTATGT GAAACTTCGC CACCTCCTAG CAAATAGTCC CAAAGTCAAA
    CAAACTGACA AACAGAAGCT GGCACAGAGG GAGGAAGCCC TCCAAAAAAT ACGGCAGAAG
    AATACAATGA GACGAGAAGT AACGGTGGAG CTAAGTAGCC AAGGATTCTG GAAAACTGGC
    ATCCGTTCTG ATGTCTGTCA GCATGCAATG ATGCTACCTG TTCTGACCCA TCATATCCGC
    TACCACCAAT GCCTAATGCA TTTGGACAAG TTGATAGGAT ATACTTTCCA AGATCGTTGT
    CTGTTGCAGC TGGCCATGAC TCATCCAAGT CATCATTTAA ATTTTGGAAT GAATCCTGAT
    CATGCCAGGA ATTCATTATC TAACTGTGGA ATTCGGCAGC CCAAATACGG AGACAGAAAA
    GTTCATCACA TGCACATGCG GAAGAAAGGG ATTAACACCT TGATAAATAT CATGTCACGC
    CTTGGCCAAG ATGACCCAAC TCCCTCGAGG ATTAACCACA ATGAACGGTT GGAATTCCTG
    GGTGATGCTG TTGTTGAATT TCTGACCAGC GTCCATTTGT ACTATTTGTT TCCTAGTCTG
    GAAGAAGGAG GATTAGCAAC CTATCGGACT GCCATTGTTC AGAATCAGCA CCTTGCCATG
    CTAGCAAAGA AACTTGAACT GGATCGATTT ATGCTGTATG CTCACGGGCC TGACCTTTGT
    AGAGAATCGG ACCTTCGACA TGCAATGGCC AATTGTTTTG AAGCGTTAAT AGGAGCTGTT
    TACTTGGAGG GAAGCCTGGA GGAAGCCAAG CAGTTATTTG GACGCTTGCT CTTTAATGAT
    CCGGACCTGC GCGAAGTCTG GCTCAATTAT CCTCTCCACC CACTCCAACT ACAAGAGCCA
    AATACTGATC GACAACTTAT TGAAACTTCT CCAGTTCTAC AAAAACTTAC TGAGTTTGAA
    GAAGCAATTG GAGTAATTTT TACTCATGTT CGACTTCTGG CAAGGGCATT CACATTGAGA
    ACTGTGGGAT TTAACCATCT GACCCTAGGC CACAATCAGA GAATGGAATT CCTAGGTGAC
    TCCATAATGC AACTGGTAGC CACAGAGTAC TTATTCATTC ATTTCCCAGA TCATCATGAA
    GGACACTTAA CTTTGTTGCG AAGCTCTTTG GTGAATAATA GAACTCAGGC CAAGGTAGCG
    GAGGAGCTGG GCATGCAGGA GTACGCCATA ACCAACGACA AGACCAAGAG GCCTGTGGCG
    CTTCGCACCA AGACCTTGGC GGACCTTTTG GAATCATTTA TTGCAGCGCT GTACATTGAT
    AAGGATTTGG AATATGTTCA TACTTTCATG AATGTCTGCT TCTTTCCACG ATTGAAAGAG
    TTCATTTTGA ATCAGGATTG GAATGACCCC AAATCCCAGC TTCAGCAGTG TTGCTTGACA
    CTTAGGACAG AAGGAAAAGA GCCAGACATT CCTCTGTACA AGACTCTGCA GACAGTGGGC
    CCATCCCATG CCCGAACCTA CACTGTGGCT GTTTATTTCA AGGGAGAAAG AATAGGCTGT
    GGGAAAGGAC CAAGTATTCA GCAAGCGGAA ATGGGAGCAG CAATGGATGC GCTTGAAAAA
    TATAATTTTC CCCAGATGGC CCATCAGAAG CGGTTCATCG AACGGAAGTA CAGACAAGAG
    TTAAAAGAAA TGAGGTGGGA AAGAGAGCAT CAAGAGAGAG AGCCAGATGA GACTGAAGAC
    ATCAAGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248350.1
    CDS436..4467
    Translation

    Target ORF information:

    RefSeq Version XM_005248293.3
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248293.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGGAGTTA CGGTTTATCG GTTGTTCCTG AACCTGCTGG ATGCACACCA
    GAATTACCTG GGGAGATTAT TAAAAATACA GATTCTTGGG CCCCACCCCT GGAGATTGTG
    AATCATCGCT CCCCAAGTAG GGAGAAGAAG AGAGCTCGTT GGGAGGAAGA AAAAGACCGT
    TGGAGTGACA ACCAGAGTTC TGGCAAAGAC AAGAACTATA CCTCAATCAA GGAAAAAGAG
    CCCGAGGAGA CCATGCCTGA CAAGAATGAG GAGGAAGAAG AAGAACTTCT TAAGCCTGTG
    TGGATTCGAT GCACTCATTC AGAAAACTAC TACTCCAGTG ACCCCATGGA TCAGGTGGGA
    GATTCTACAG TGGTTGGAAC GAGTAGGCTT CGTGACTTAT ATGACAAATT TGAGGAGGAG
    TTGGGGAGCA GGCAAGAAAA GGCCAAAGCT GCTCGGCCTC CGTGGGAACC TCCAAAGACG
    AAGCTCGATG AAGATTTAGA GAGTTCCAGT GAATCCGAGT GTGAGTCTGA TGAGGACAGC
    ACCTGTTCTA GCAGCTCAGA CTCTGAAGTT TTTGACGTTA TTGCAGAAAT CAAACGCAAA
    AAGGCCCACC CTGACCGACT TCATGATGAA CTTTGGTACA ACGATCCAGG CCAGATGAAT
    GATGGACCAC TCTGCAAATG CAGCGCAAAG GCAAGACGCA CAGGAATTAG GCACAGCATT
    TATCCTGGAG AAGAGGCCAT CAAGCCCTGT CGTCCTATGA CCAACAATGC TGGCAGACTT
    TTCCACTACC GGATCACAGT CTCCCCGCCT ACGAACTTTT TAACTGACAG GCCAACTGTT
    ATAGAATACG ATGATCACGA GTATATCTTT GAAGGATTTT CTATGTTTGC ACATGCCCCC
    CTGACCAATA TTCCACTGTG TAAAGTAATT AGATTCAACA TAGACTACAC GATTCATTTC
    ATTGAAGAGA TGATGCCGGA GAATTTTTGT GTGAAAGGGC TTGAACTCTT TTCACTGTTC
    CTATTCAGAG ATATTTTGGA ATTATATGAC TGGAATCTTA AAGGTCCTTT GTTTGAAGAC
    AGCCCTCCCT GCTGCCCAAG ATTTCATTTC ATGCCACGTT TTGTAAGATT TCTTCCAGAT
    GGAGGAAAGG AAGTGCTGTC CATGCACCAG ATTCTCCTGT ACTTGTTAAG GTGCAGCAAA
    GCCCTGGTGC CTGAGGAGGA GATTGCCAAT ATGCTTCAGT GGGAGGAGCT GGAGTGGCAG
    AAATATGCAG AAGAATGCAA AGGCATGATT GTTACCAACC CTGGGACGAA ACCAAGCTCT
    GTCCGTATCG ATCAACTGGA TCGTGAACAG TTCAACCCCG ATGTGATTAC TTTTCCGATT
    ATCGTCCACT TTGGGATACG CCCTGCACAG TTGAGTTATG CAGGAGACCC ACAGTACCAA
    AAACTGTGGA AGAGTTATGT GAAACTTCGC CACCTCCTAG CAAATAGTCC CAAAGTCAAA
    CAAACTGACA AACAGAAGCT GGCACAGAGG GAGGAAGCCC TCCAAAAAAT ACGGCAGAAG
    AATACAATGA GACGAGAAGT AACGGTGGAG CTAAGTAGCC AAGGATTCTG GAAAACTGGC
    ATCCGTTCTG ATGTCTGTCA GCATGCAATG ATGCTACCTG TTCTGACCCA TCATATCCGC
    TACCACCAAT GCCTAATGCA TTTGGACAAG TTGATAGGAT ATACTTTCCA AGATCGTTGT
    CTGTTGCAGC TGGCCATGAC TCATCCAAGT CATCATTTAA ATTTTGGAAT GAATCCTGAT
    CATGCCAGGA ATTCATTATC TAACTGTGGA ATTCGGCAGC CCAAATACGG AGACAGAAAA
    GTTCATCACA TGCACATGCG GAAGAAAGGG ATTAACACCT TGATAAATAT CATGTCACGC
    CTTGGCCAAG ATGACCCAAC TCCCTCGAGG ATTAACCACA ATGAACGGTT GGAATTCCTG
    GGTGATGCTG TTGTTGAATT TCTGACCAGC GTCCATTTGT ACTATTTGTT TCCTAGTCTG
    GAAGAAGGAG GATTAGCAAC CTATCGGACT GCCATTGTTC AGAATCAGCA CCTTGCCATG
    CTAGCAAAGA AACTTGAACT GGATCGATTT ATGCTGTATG CTCACGGGCC TGACCTTTGT
    AGAGAATCGG ACCTTCGACA TGCAATGGCC AATTGTTTTG AAGCGTTAAT AGGAGCTGTT
    TACTTGGAGG GAAGCCTGGA GGAAGCCAAG CAGTTATTTG GACGCTTGCT CTTTAATGAT
    CCGGACCTGC GCGAAGTCTG GCTCAATTAT CCTCTCCACC CACTCCAACT ACAAGAGCCA
    AATACTGATC GACAACTTAT TGAAACTTCT CCAGTTCTAC AAAAACTTAC TGAGTTTGAA
    GAAGCAATTG GAGTAATTTT TACTCATGTT CGACTTCTGG CAAGGGCATT CACATTGAGA
    ACTGTGGGAT TTAACCATCT GACCCTAGGC CACAATCAGA GAATGGAATT CCTAGGTGAC
    TCCATAATGC AACTGGTAGC CACAGAGTAC TTATTCATTC ATTTCCCAGA TCATCATGAA
    GGACACTTAA CTTTGTTGCG AAGCTCTTTG GTGAATAATA GAACTCAGGC CAAGGTAGCG
    GAGGAGCTGG GCATGCAGGA GTACGCCATA ACCAACGACA AGACCAAGAG GCCTGTGGCG
    CTTCGCACCA AGACCTTGGC GGACCTTTTG GAATCATTTA TTGCAGCGCT GTACATTGAT
    AAGGATTTGG AATATGTTCA TACTTTCATG AATGTCTGCT TCTTTCCACG ATTGAAAGAG
    TTCATTTTGA ATCAGGATTG GAATGACCCC AAATCCCAGC TTCAGCAGTG TTGCTTGACA
    CTTAGGACAG AAGGAAAAGA GCCAGACATT CCTCTGTACA AGACTCTGCA GACAGTGGGC
    CCATCCCATG CCCGAACCTA CACTGTGGCT GTTTATTTCA AGGGAGAAAG AATAGGCTGT
    GGGAAAGGAC CAAGTATTCA GCAAGCGGAA ATGGGAGCAG CAATGGATGC GCTTGAAAAA
    TATAATTTTC CCCAGATGGC CCATCAGAAG CGGTTCATCG AACGGAAGTA CAGACAAGAG
    TTAAAAGAAA TGAGGTGGGA AAGAGAGCAT CAAGAGAGAG AGCCAGATGA GACTGAAGAC
    ATCAAGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39315
    Clone ID Related Accession (Same CDS sequence) XM_005248293.3 , XM_005248293.4
    Accession Version XM_005248293.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ribonuclease 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248293.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGGAGTTA CGGTTTATCG GTTGTTCCTG AACCTGCTGG ATGCACACCA
    GAATTACCTG GGGAGATTAT TAAAAATACA GATTCTTGGG CCCCACCCCT GGAGATTGTG
    AATCATCGCT CCCCAAGTAG GGAGAAGAAG AGAGCTCGTT GGGAGGAAGA AAAAGACCGT
    TGGAGTGACA ACCAGAGTTC TGGCAAAGAC AAGAACTATA CCTCAATCAA GGAAAAAGAG
    CCCGAGGAGA CCATGCCTGA CAAGAATGAG GAGGAAGAAG AAGAACTTCT TAAGCCTGTG
    TGGATTCGAT GCACTCATTC AGAAAACTAC TACTCCAGTG ACCCCATGGA TCAGGTGGGA
    GATTCTACAG TGGTTGGAAC GAGTAGGCTT CGTGACTTAT ATGACAAATT TGAGGAGGAG
    TTGGGGAGCA GGCAAGAAAA GGCCAAAGCT GCTCGGCCTC CGTGGGAACC TCCAAAGACG
    AAGCTCGATG AAGATTTAGA GAGTTCCAGT GAATCCGAGT GTGAGTCTGA TGAGGACAGC
    ACCTGTTCTA GCAGCTCAGA CTCTGAAGTT TTTGACGTTA TTGCAGAAAT CAAACGCAAA
    AAGGCCCACC CTGACCGACT TCATGATGAA CTTTGGTACA ACGATCCAGG CCAGATGAAT
    GATGGACCAC TCTGCAAATG CAGCGCAAAG GCAAGACGCA CAGGAATTAG GCACAGCATT
    TATCCTGGAG AAGAGGCCAT CAAGCCCTGT CGTCCTATGA CCAACAATGC TGGCAGACTT
    TTCCACTACC GGATCACAGT CTCCCCGCCT ACGAACTTTT TAACTGACAG GCCAACTGTT
    ATAGAATACG ATGATCACGA GTATATCTTT GAAGGATTTT CTATGTTTGC ACATGCCCCC
    CTGACCAATA TTCCACTGTG TAAAGTAATT AGATTCAACA TAGACTACAC GATTCATTTC
    ATTGAAGAGA TGATGCCGGA GAATTTTTGT GTGAAAGGGC TTGAACTCTT TTCACTGTTC
    CTATTCAGAG ATATTTTGGA ATTATATGAC TGGAATCTTA AAGGTCCTTT GTTTGAAGAC
    AGCCCTCCCT GCTGCCCAAG ATTTCATTTC ATGCCACGTT TTGTAAGATT TCTTCCAGAT
    GGAGGAAAGG AAGTGCTGTC CATGCACCAG ATTCTCCTGT ACTTGTTAAG GTGCAGCAAA
    GCCCTGGTGC CTGAGGAGGA GATTGCCAAT ATGCTTCAGT GGGAGGAGCT GGAGTGGCAG
    AAATATGCAG AAGAATGCAA AGGCATGATT GTTACCAACC CTGGGACGAA ACCAAGCTCT
    GTCCGTATCG ATCAACTGGA TCGTGAACAG TTCAACCCCG ATGTGATTAC TTTTCCGATT
    ATCGTCCACT TTGGGATACG CCCTGCACAG TTGAGTTATG CAGGAGACCC ACAGTACCAA
    AAACTGTGGA AGAGTTATGT GAAACTTCGC CACCTCCTAG CAAATAGTCC CAAAGTCAAA
    CAAACTGACA AACAGAAGCT GGCACAGAGG GAGGAAGCCC TCCAAAAAAT ACGGCAGAAG
    AATACAATGA GACGAGAAGT AACGGTGGAG CTAAGTAGCC AAGGATTCTG GAAAACTGGC
    ATCCGTTCTG ATGTCTGTCA GCATGCAATG ATGCTACCTG TTCTGACCCA TCATATCCGC
    TACCACCAAT GCCTAATGCA TTTGGACAAG TTGATAGGAT ATACTTTCCA AGATCGTTGT
    CTGTTGCAGC TGGCCATGAC TCATCCAAGT CATCATTTAA ATTTTGGAAT GAATCCTGAT
    CATGCCAGGA ATTCATTATC TAACTGTGGA ATTCGGCAGC CCAAATACGG AGACAGAAAA
    GTTCATCACA TGCACATGCG GAAGAAAGGG ATTAACACCT TGATAAATAT CATGTCACGC
    CTTGGCCAAG ATGACCCAAC TCCCTCGAGG ATTAACCACA ATGAACGGTT GGAATTCCTG
    GGTGATGCTG TTGTTGAATT TCTGACCAGC GTCCATTTGT ACTATTTGTT TCCTAGTCTG
    GAAGAAGGAG GATTAGCAAC CTATCGGACT GCCATTGTTC AGAATCAGCA CCTTGCCATG
    CTAGCAAAGA AACTTGAACT GGATCGATTT ATGCTGTATG CTCACGGGCC TGACCTTTGT
    AGAGAATCGG ACCTTCGACA TGCAATGGCC AATTGTTTTG AAGCGTTAAT AGGAGCTGTT
    TACTTGGAGG GAAGCCTGGA GGAAGCCAAG CAGTTATTTG GACGCTTGCT CTTTAATGAT
    CCGGACCTGC GCGAAGTCTG GCTCAATTAT CCTCTCCACC CACTCCAACT ACAAGAGCCA
    AATACTGATC GACAACTTAT TGAAACTTCT CCAGTTCTAC AAAAACTTAC TGAGTTTGAA
    GAAGCAATTG GAGTAATTTT TACTCATGTT CGACTTCTGG CAAGGGCATT CACATTGAGA
    ACTGTGGGAT TTAACCATCT GACCCTAGGC CACAATCAGA GAATGGAATT CCTAGGTGAC
    TCCATAATGC AACTGGTAGC CACAGAGTAC TTATTCATTC ATTTCCCAGA TCATCATGAA
    GGACACTTAA CTTTGTTGCG AAGCTCTTTG GTGAATAATA GAACTCAGGC CAAGGTAGCG
    GAGGAGCTGG GCATGCAGGA GTACGCCATA ACCAACGACA AGACCAAGAG GCCTGTGGCG
    CTTCGCACCA AGACCTTGGC GGACCTTTTG GAATCATTTA TTGCAGCGCT GTACATTGAT
    AAGGATTTGG AATATGTTCA TACTTTCATG AATGTCTGCT TCTTTCCACG ATTGAAAGAG
    TTCATTTTGA ATCAGGATTG GAATGACCCC AAATCCCAGC TTCAGCAGTG TTGCTTGACA
    CTTAGGACAG AAGGAAAAGA GCCAGACATT CCTCTGTACA AGACTCTGCA GACAGTGGGC
    CCATCCCATG CCCGAACCTA CACTGTGGCT GTTTATTTCA AGGGAGAAAG AATAGGCTGT
    GGGAAAGGAC CAAGTATTCA GCAAGCGGAA ATGGGAGCAG CAATGGATGC GCTTGAAAAA
    TATAATTTTC CCCAGATGGC CCATCAGAAG CGGTTCATCG AACGGAAGTA CAGACAAGAG
    TTAAAAGAAA TGAGGTGGGA AAGAGAGCAT CAAGAGAGAG AGCCAGATGA GACTGAAGAC
    ATCAAGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248350.1
    CDS403..4434
    Translation

    Target ORF information:

    RefSeq Version XM_005248293.4
    Organism Homo sapiens(human)
    Definition Homo sapiens drosha ribonuclease III (DROSHA), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248293.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGATGCAGG GAAACACATG TCACAGAATG TCGTTCCACC CGGGACGAGG GTGTCCCCGA 
    GGACGAGGAG GACATGGAGC CAGACCCTCA GCACCATCCT TTAGGCCCCA AAATCTGAGG
    CTGCTTCACC CTCAGCAGCC TCCTGTGCAA TATCAATATG AACCTCCAAG TGCCCCTTCC
    ACCACTTTCT CAAACTCTCC AGCCCCCAAT TTTCTCCCTC CACGACCAGA CTTTGTACCC
    TTCCCCCCAC CCATGCCTCC GTCAGCGCAA GGCCCTCTTC CCCCCTGCCC AATCAGGCCG
    CCTTTCCCCA ACCACCAGAT GAGGCACCCC TTCCCAGTTC CTCCTTGTTT TCCTCCCATG
    CCACCACCAA TGCCTTGTCC TAATAACCCC CCAGTCCCTG GGGCACCTCC TGGACAAGGC
    ACTTTCCCCT TCATGATGCC CCCTCCCTCC ATGCCTCATC CCCCGCCCCC TCCAGTCATG
    CCGCAGCAGG TTAATTATCA GTACCCTCCG GGCTATTCTC ACCACAACTT CCCACCTCCC
    AGTTTTAATA GTTTCCAGAA CAACCCTAGT TCTTTCCTGC CCAGTGCTAA TAACAGCAGT
    AGTCCTCATT TCAGACATCT CCCTCCATAC CCACTCCCAA AGGCTCCCAG TGAGAGAAGG
    TCCCCAGAAA GGCTGAAACA CTATGATGAC CACAGGCACC GAGATCACAG TCATGGGCGA
    GGTGAGAGGC ATCGGTCCCT GGATCGGCGG GAGCGAGGCC GCAGTCCCGA CAGGAGAAGA
    CAAGACAGCC GGTACAGATC TGATTATGAC CGAGGGAGAA CACCATCTCG CCACCGCAGC
    TACGAACGGA GCAGGAGTTA CGGTTTATCG GTTGTTCCTG AACCTGCTGG ATGCACACCA
    GAATTACCTG GGGAGATTAT TAAAAATACA GATTCTTGGG CCCCACCCCT GGAGATTGTG
    AATCATCGCT CCCCAAGTAG GGAGAAGAAG AGAGCTCGTT GGGAGGAAGA AAAAGACCGT
    TGGAGTGACA ACCAGAGTTC TGGCAAAGAC AAGAACTATA CCTCAATCAA GGAAAAAGAG
    CCCGAGGAGA CCATGCCTGA CAAGAATGAG GAGGAAGAAG AAGAACTTCT TAAGCCTGTG
    TGGATTCGAT GCACTCATTC AGAAAACTAC TACTCCAGTG ACCCCATGGA TCAGGTGGGA
    GATTCTACAG TGGTTGGAAC GAGTAGGCTT CGTGACTTAT ATGACAAATT TGAGGAGGAG
    TTGGGGAGCA GGCAAGAAAA GGCCAAAGCT GCTCGGCCTC CGTGGGAACC TCCAAAGACG
    AAGCTCGATG AAGATTTAGA GAGTTCCAGT GAATCCGAGT GTGAGTCTGA TGAGGACAGC
    ACCTGTTCTA GCAGCTCAGA CTCTGAAGTT TTTGACGTTA TTGCAGAAAT CAAACGCAAA
    AAGGCCCACC CTGACCGACT TCATGATGAA CTTTGGTACA ACGATCCAGG CCAGATGAAT
    GATGGACCAC TCTGCAAATG CAGCGCAAAG GCAAGACGCA CAGGAATTAG GCACAGCATT
    TATCCTGGAG AAGAGGCCAT CAAGCCCTGT CGTCCTATGA CCAACAATGC TGGCAGACTT
    TTCCACTACC GGATCACAGT CTCCCCGCCT ACGAACTTTT TAACTGACAG GCCAACTGTT
    ATAGAATACG ATGATCACGA GTATATCTTT GAAGGATTTT CTATGTTTGC ACATGCCCCC
    CTGACCAATA TTCCACTGTG TAAAGTAATT AGATTCAACA TAGACTACAC GATTCATTTC
    ATTGAAGAGA TGATGCCGGA GAATTTTTGT GTGAAAGGGC TTGAACTCTT TTCACTGTTC
    CTATTCAGAG ATATTTTGGA ATTATATGAC TGGAATCTTA AAGGTCCTTT GTTTGAAGAC
    AGCCCTCCCT GCTGCCCAAG ATTTCATTTC ATGCCACGTT TTGTAAGATT TCTTCCAGAT
    GGAGGAAAGG AAGTGCTGTC CATGCACCAG ATTCTCCTGT ACTTGTTAAG GTGCAGCAAA
    GCCCTGGTGC CTGAGGAGGA GATTGCCAAT ATGCTTCAGT GGGAGGAGCT GGAGTGGCAG
    AAATATGCAG AAGAATGCAA AGGCATGATT GTTACCAACC CTGGGACGAA ACCAAGCTCT
    GTCCGTATCG ATCAACTGGA TCGTGAACAG TTCAACCCCG ATGTGATTAC TTTTCCGATT
    ATCGTCCACT TTGGGATACG CCCTGCACAG TTGAGTTATG CAGGAGACCC ACAGTACCAA
    AAACTGTGGA AGAGTTATGT GAAACTTCGC CACCTCCTAG CAAATAGTCC CAAAGTCAAA
    CAAACTGACA AACAGAAGCT GGCACAGAGG GAGGAAGCCC TCCAAAAAAT ACGGCAGAAG
    AATACAATGA GACGAGAAGT AACGGTGGAG CTAAGTAGCC AAGGATTCTG GAAAACTGGC
    ATCCGTTCTG ATGTCTGTCA GCATGCAATG ATGCTACCTG TTCTGACCCA TCATATCCGC
    TACCACCAAT GCCTAATGCA TTTGGACAAG TTGATAGGAT ATACTTTCCA AGATCGTTGT
    CTGTTGCAGC TGGCCATGAC TCATCCAAGT CATCATTTAA ATTTTGGAAT GAATCCTGAT
    CATGCCAGGA ATTCATTATC TAACTGTGGA ATTCGGCAGC CCAAATACGG AGACAGAAAA
    GTTCATCACA TGCACATGCG GAAGAAAGGG ATTAACACCT TGATAAATAT CATGTCACGC
    CTTGGCCAAG ATGACCCAAC TCCCTCGAGG ATTAACCACA ATGAACGGTT GGAATTCCTG
    GGTGATGCTG TTGTTGAATT TCTGACCAGC GTCCATTTGT ACTATTTGTT TCCTAGTCTG
    GAAGAAGGAG GATTAGCAAC CTATCGGACT GCCATTGTTC AGAATCAGCA CCTTGCCATG
    CTAGCAAAGA AACTTGAACT GGATCGATTT ATGCTGTATG CTCACGGGCC TGACCTTTGT
    AGAGAATCGG ACCTTCGACA TGCAATGGCC AATTGTTTTG AAGCGTTAAT AGGAGCTGTT
    TACTTGGAGG GAAGCCTGGA GGAAGCCAAG CAGTTATTTG GACGCTTGCT CTTTAATGAT
    CCGGACCTGC GCGAAGTCTG GCTCAATTAT CCTCTCCACC CACTCCAACT ACAAGAGCCA
    AATACTGATC GACAACTTAT TGAAACTTCT CCAGTTCTAC AAAAACTTAC TGAGTTTGAA
    GAAGCAATTG GAGTAATTTT TACTCATGTT CGACTTCTGG CAAGGGCATT CACATTGAGA
    ACTGTGGGAT TTAACCATCT GACCCTAGGC CACAATCAGA GAATGGAATT CCTAGGTGAC
    TCCATAATGC AACTGGTAGC CACAGAGTAC TTATTCATTC ATTTCCCAGA TCATCATGAA
    GGACACTTAA CTTTGTTGCG AAGCTCTTTG GTGAATAATA GAACTCAGGC CAAGGTAGCG
    GAGGAGCTGG GCATGCAGGA GTACGCCATA ACCAACGACA AGACCAAGAG GCCTGTGGCG
    CTTCGCACCA AGACCTTGGC GGACCTTTTG GAATCATTTA TTGCAGCGCT GTACATTGAT
    AAGGATTTGG AATATGTTCA TACTTTCATG AATGTCTGCT TCTTTCCACG ATTGAAAGAG
    TTCATTTTGA ATCAGGATTG GAATGACCCC AAATCCCAGC TTCAGCAGTG TTGCTTGACA
    CTTAGGACAG AAGGAAAAGA GCCAGACATT CCTCTGTACA AGACTCTGCA GACAGTGGGC
    CCATCCCATG CCCGAACCTA CACTGTGGCT GTTTATTTCA AGGGAGAAAG AATAGGCTGT
    GGGAAAGGAC CAAGTATTCA GCAAGCGGAA ATGGGAGCAG CAATGGATGC GCTTGAAAAA
    TATAATTTTC CCCAGATGGC CCATCAGAAG CGGTTCATCG AACGGAAGTA CAGACAAGAG
    TTAAAAGAAA TGAGGTGGGA AAGAGAGCAT CAAGAGAGAG AGCCAGATGA GACTGAAGAC
    ATCAAGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.