DROSHA cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	DROSHA
Entrez Gene ID	420911
Full Name	drosha ribonuclease III
Synonyms	RNASEN
General protein information	Preferred Names drosha ribonuclease III Names ribonuclease 3 drosha, ribonuclease type III ribonuclease III, nuclear ribonuclease type III, nuclear
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015282183.1	XP_015137669.1	ribonuclease 3 isoform X1
XM_015282183.2	XP_015137669.1	ribonuclease 3 isoform X1
XM_025147270.1	XP_025003038.1	ribonuclease 3 isoform X1
XM_015282182.1	XP_015137668.1	ribonuclease 3 isoform X1
NM_001006379.1	NP_001006379.1	ribonuclease 3
XM_015282182.2	XP_015137668.1	ribonuclease 3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DROSHA	NP_037367.3
Mus musculus (house mouse)	Drosha	NP_001123621.1
Rattus norvegicus (Norway rat)	Drosha	NP_001101125.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008087	XP_555381.3
	DROSHA	XP_002804372.1
Caenorhabditis elegans (roundworm)	drsh-1	NP_492599.1
Xenopus tropicalis (tropical clawed frog)	drosha	NP_001107152.1
Pan troglodytes (chimpanzee)	DROSHA	XP_001157472.2
Bos taurus (cattle)	DROSHA	XP_005221686.1
Canis lupus familiaris (dog)	DROSHA	XP_854135.2
Gallus gallus (chicken)	DROSHA	NP_001006379.1
Danio rerio (zebrafish)	rnasen	NP_001103942.1
Drosophila melanogaster (fruit fly)	drosha	NP_477436.1

Gene Ontology
Bibliography

Related articles in PubMed

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P, Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci P, Hayashizaki Y, Buerstedde JM
Genome biology6(1)R6(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DROSHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DROSHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa32168	XM_025147270.1 Latest version!	Gallus gallus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa32168	XM_015282183.2 Latest version!	Gallus gallus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa32168	XM_015282182.2 Latest version!	Gallus gallus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa25819	NM_001006379.1 Latest version!	Gallus gallus drosha ribonuclease III (DROSHA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OGa32168	XM_015282183.1	Gallus gallus drosha, ribonuclease type III (DROSHA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa32168	XM_015282182.1	Gallus gallus drosha, ribonuclease type III (DROSHA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DROSHA ( XM_015282183.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137669.1 Gallus gallus drosha, ribonuclease type III (DROSHA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa32168
Clone ID Related Accession (Same CDS sequence)	XM_015282183.1 , XM_015282183.2 , XM_025147270.1 , XM_015282182.1 , XM_015282182.2
Accession Version	XM_015282183.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ribonuclease 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455777.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTCC ATTCTGGACG AGGATGTCCC CGTGGGCAGG GGGGACCAGG AGCAAGAACT 
TCAGCACAGA CGTATCGTCC TCAGAACCTG CGGCAGCTTC ACCCCCAGCA GCCTTCTGTA 
CAGTACCAGT ATGACCAGCA AGCCGCACCT GCAGCTACCT ATTCAAACCC TTCAGCCACC 
AGTTACATGC CTCCCAGGCC AGACTTTGTT CCATATCCCC CACCAGTTCC CCCTTCCACA 
CAAAATCCCA TCAACCAGTG TCCCATGAGG CCACCGTTTC CAAACCATCA GATGAGGCAG 
AGCTTCCCAG TGCCTCCATG CTTCCCTCCT GCTCCTCCAC CAGTGCCAGC CCCTAGCAAT 
GCTCCCGTGC CAGCAACTGC TACTGGGCAG AGCACCTTTC CATACATGAT GCCTCCTCCC 
GCAGTACCCC ATCCTCCCCC ACCACCAGTC ATACCACAGC AAGTCAACTA CCAGCCTGTA 
TATTCCACAG GGTATTCCCA GCAGTCATTC CCACCACCTA ACTTCAATAG CTATCAGCAC 
AACTCTGGCT CCTTTCAGAG CAGCACTACC AATAGCAGCA GTGGCAGCAG CCATTTTCGG 
CACACGGGTC AGTACCAGGG AGAAAAGTCA CAAAGTGATC GACGATCTCC GGAGAGGAGC 
AAGCACTACG ACGAGCACCG GCACCGTGAG CACAGTCACA TCCACGGTGA CAGGCATCGA 
TCCAATAACC ACGGGGATCG TCGGGATCGA GGCCGCAGTC CAGACAGGAG GAGGCAGGAA 
AGCAGTCGGC ACCGGACTGA ATATGACAGA GGAAGAATGT CGCCTCATCA TAGAAGTTAT 
GAGCGTAGCA GGGAGCGTGA GAGGGACAGG CATAGGCACC GTGACAGCAG AAGATCACCA 
TCACCGGACA GGTCCTACAA AAAAGATTAT AAAAGAGCAG GAAGCCGCTC TCCAAGTAGA 
GAAAGAAAGA GACCCCGATG GGAGGAGGAA AGAGAAAGGT GGTCTGAGAG CCAGAGTGCC 
AACAAAGAAA AAAACTACAC TAGTATCAAA GACAAAGAGT CAGAGGAGAA TGCATCTGAA 
AAAAATGAAG ATGAAGATGA TGAATTACTT AAACCAGTCT GGGTTCGCTG TACCCATTCT 
GAAAGCTATT ATTCAAATGA CCCCATGGAT CAAGTGGGGG ACTCTACTGT GGTAGGCACA 
AGCAAGCTCC GAGATCTGTA TGAGAAGTTT GAGGAGGAGC TAGGAAAACG ACAGGCAAAG 
GCCAAAGCAG CCAGGCCACC ATGGGAGCCG CCGAAGACCA AGCTGGATGA AGATCTTGAG 
AGCTCCAGTG AGTCAGAATG CGACTCTGAT GATGACAGCA GCTGCTCCAG CAGTTCAGAT 
TCGGAAGTTT TTGATGTCAT TGCAGAAATT AAGCGTAAGA AAGCACACCC TGATCGTCTT 
CATGAGGAAC TGTGGTACAA TGATCCAGGA CAGATGAATG ATGGACCGCT GTGCAAGTGC 
AGCGCTAAAG CCCGACGAAC AGGGATAAGA CATGGCATTT ATCCTGGTGA AGAGCCCATT 
AAACCATGTC GCCCCATGAC CAACAATGCT GGAAGACTGT ACCATTATCG AATTACAGTG 
TCACCTCCCA CAAACTTCTT AACAGACAGA CCTACTGTTA TTGAGTATGA TGACCATGAA 
TATATCTTCG AAGGATTCTC CATGTTTTCA CATGCCCCAC TGACAAACAT TCCTCTATGT 
AAAGTAATCA GATTTAACAT TGACTATACA ATTCATTTCA TTGAAGAGAT GATGCCTGAG 
AATTTTTGTG TGAAAGGTCT TGAGCTGTTC TCTTCTTATC TATTCAAAGA TATTCTGGAG 
CTGTATGACT GGCATCTTAA AGGTCCTTCA GTTGAAAATG ATTCTGTTTC CTGCCCCAGA 
TTTCACTTCA TGCCGCGATT TGTGAGATTT CTTCCAGATG GAGGGAAGGA AGTACTGTCA 
ATGCATCAGA TTCTTCTCTA CTTGTTACGA TGCAATAAAG CTTTGGTGCC TGAAGAAGAG 
ATTGCCAACA TGCTTCAGTG GGAAGAGCTG GAATGGCAGA AATATGCAGA GGAATGCAAA 
GGGATGATCG TTACCAGCCC TGGCATGAAA CCCAGCTCAG TTCGTATTGA TCAACTGGAC 
CGTGAACAAT TCAATCCTGA TGTAATTACT TTCCCCATTA TTGTCCACTT TGGGATACGA 
CCTGCTCAAC TCAGCTACGC TGGAGATCCT CAATACCAAA AACTATGGAA GAGTTATGTG 
AAGCTGCGTC ACCTGCTAGC TAACAGTCCC AAAGTCAAAC AAGCTGATAA ACAGAAATTA 
TCACAGAGAG AGGAAGCACT GCAGAAAATC CGGCAGAAGA ACACGATGAG ACGTGAAGTG 
ACTGTCGAGT TGAGTAGCCA GGGATTCTGG AAGACGGGGA TACGCTCTGA TGTCTGCCAG 
CACGCAATGA TGCTTCCTGT CCTCACCCAC CATATTCGCT ACCATCAGTG TCTGATGCAT 
TTGGACAAGT TGATAGGCTA CACTTTTCGA GATAGGTGCC TGCTGCAGCT TGCCATGACT 
CATCCGAGCC ATCACTTAAA CTTTGGAATG AATCCAGACC ATGCCAGAAA TTCCTTATCC 
AACTGTGGGA TTCGACAGCC AAAGTATGGG GACAGAAAAG TTCACCATAT GCATATGAGA 
AAAAAAGGGA TAAACACCCT GATAAATATT ATGTCCCGTT TGGGACAGGA TGATCCAGCT 
CCTTCCAGGA TTAACCACAA TGAGCGTTTA GAATTTCTGG GAGATGCTGT GGTGGAGTTC 
TTAACAAGTG TCCACTTGTA CTACCTGTTT CCCACTCTGG AGGAAGGAGG ATTAGCTACG 
TACCGCACTG CCATAGTTCA GAACCAGCAT CTGGCCATGC TGGCTAAGAA GCTGGAACTA 
GATCGATTTA TGCTTTATGC TCATGGTCCG GACCTTTGCA GGGAATCGGA TCTCCGCCAT 
GCCATGGCCA ACTGCTTTGA AGCACTAATA GGAGCAGTTT ATCTGGAGGG TAGTCTTGAA 
GAAGCCAAAC AATTATTTGG ACGGTTGCTC TTCAATGACA AGGACCTGCG GGATGTATGG 
CTTAATTATC CACTACACCC ACTCCAACTG CAGGAGTCTA ATACTGACAG GCAACTCATT 
GAGACCTCTC CAGTTCTTCA AAAGCTTACG GAGTTTGAGG AGGCAATTGG AGTCATCTTT 
ACTCATGTTC GACTTCTAGC ACGTGCATTC ACTCTGAGGA CAGTAGGCTT TAACCACCTG 
ACTCTAGGCC ACAATCAGAG GATGGAATTC CTGGGTGACT CCATAATGCA GTTGGTAGCC 
ACAGAGTATT TATTCATACA TTTCCCCGAT CATCATGAAG GGCACTTAAC GCTGTTGAGA 
AGCTCTCTGG TGAACAACAG GACTCAGGCC AAAGTGGCAG AAGAGCTGGG AATGCAAGAG 
TTTGCCATCA CTAATGACAA GACCAAAAGA CCCGTAGCTC TTCGAACTAA AACTCTGGCA 
GATCTCTTGG AATCCTTTAT TGCTGCCCTG TACATTGATA AAGATTTGGA ATATGTTCAC 
ACTTTCATGA ATGTATGCTT TTTTCCACGA CTAAAGGAAT TCATTTTAAA TCAAGATTGG 
AATGACCCTA AATCTCAGCT TCAGCAGTGC TGCTTGACTC TCAGGACAGA AGGAAAGGAG 
CCAGACATTC CTCTTTACAA GACTCTGCAG ACAGTGGGAC CATCTCATGC CAGGACGTAC 
ACTGTAGCTG TTTATTTCAA AGGGGAAAGA ATAGGATGTG GTAAAGGCCC AAGTATTCAA 
CAAGCAGAAA TGGGAGCTGC AATGGATGCA CTTGAAAAAT ATAACTTTCC CCAGATGGCC 
CATCAGAAAC GGTTCATTGA ACGGAAGTAC AGACAAGAGT TGAAAGAAAT GAGGTGGGAA 
AGAGAGCATC AAGAGAGAGA AACAGATGAG ACTGAAGAAG CAAGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137669.1
CDS	240..4250
Translation	MSFHSGRGCPRGQGGPGARTSAQTYRPQNLRQLHPQQPSVQYQYDQQAAPAATYSNPSATSYMPPRPDFVPYPPPVPPSTQNPINQCPMRPPFPNHQMRQSFPVPPCFPPAPPPVPAPSNAPVPATATGQSTFPYMMPPPAVPHPPPPPVIPQQVNYQPVYSTGYSQQSFPPPNFNSYQHNSGSFQSSTTNSSSGSSHFRHTGQYQGEKSQSDRRSPERSKHYDEHRHREHSHIHGDRHRSNNHGDRRDRGRSPDRRRQESSRHRTEYDRGRMSPHHRSYERSRERERDRHRHRDSRRSPSPDRSYKKDYKRAGSRSPSRERKRPRWEEERERWSESQSANKEKNYTSIKDKESEENASEKNEDEDDELLKPVWVRCTHSESYYSNDPMDQVGDSTVVGTSKLRDLYEKFEEELGKRQAKAKAARPPWEPPKTKLDEDLESSSESECDSDDDSSCSSSSDSEVFDVIAEIKRKKAHPDRLHEELWYNDPGQMNDGPLCKCSAKARRTGIRHGIYPGEEPIKPCRPMTNNAGRLYHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFSHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSSYLFKDILELYDWHLKGPSVENDSVSCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCNKALVPEEEIANMLQWEELEWQKYAEECKGMIVTSPGMKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQADKQKLSQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFRDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPAPSRINHNERLEFLGDAVVEFLTSVHLYYLFPTLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDKDLRDVWLNYPLHPLQLQESNTDRQLIETSPVLQKLTEFEEAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEFAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQERETDETEEARK

Target ORF information:

RefSeq Version	XM_015282183.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus drosha, ribonuclease type III (DROSHA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282183.1

ORF Insert Sequence:

ATGTCTTTCC ATTCTGGACG AGGATGTCCC CGTGGGCAGG GGGGACCAGG AGCAAGAACT 
TCAGCACAGA CGTATCGTCC TCAGAACCTG CGGCAGCTTC ACCCCCAGCA GCCTTCTGTA 
CAGTACCAGT ATGACCAGCA AGCCGCACCT GCAGCTACCT ATTCAAACCC TTCAGCCACC 
AGTTACATGC CTCCCAGGCC AGACTTTGTT CCATATCCCC CACCAGTTCC CCCTTCCACA 
CAAAATCCCA TCAACCAGTG TCCCATGAGG CCACCGTTTC CAAACCATCA GATGAGGCAG 
AGCTTCCCAG TGCCTCCATG CTTCCCTCCT GCTCCTCCAC CAGTGCCAGC CCCTAGCAAT 
GCTCCCGTGC CAGCAACTGC TACTGGGCAG AGCACCTTTC CATACATGAT GCCTCCTCCC 
GCAGTACCCC ATCCTCCCCC ACCACCAGTC ATACCACAGC AAGTCAACTA CCAGCCTGTA 
TATTCCACAG GGTATTCCCA GCAGTCATTC CCACCACCTA ACTTCAATAG CTATCAGCAC 
AACTCTGGCT CCTTTCAGAG CAGCACTACC AATAGCAGCA GTGGCAGCAG CCATTTTCGG 
CACACGGGTC AGTACCAGGG AGAAAAGTCA CAAAGTGATC GACGATCTCC GGAGAGGAGC 
AAGCACTACG ACGAGCACCG GCACCGTGAG CACAGTCACA TCCACGGTGA CAGGCATCGA 
TCCAATAACC ACGGGGATCG TCGGGATCGA GGCCGCAGTC CAGACAGGAG GAGGCAGGAA 
AGCAGTCGGC ACCGGACTGA ATATGACAGA GGAAGAATGT CGCCTCATCA TAGAAGTTAT 
GAGCGTAGCA GGGAGCGTGA GAGGGACAGG CATAGGCACC GTGACAGCAG AAGATCACCA 
TCACCGGACA GGTCCTACAA AAAAGATTAT AAAAGAGCAG GAAGCCGCTC TCCAAGTAGA 
GAAAGAAAGA GACCCCGATG GGAGGAGGAA AGAGAAAGGT GGTCTGAGAG CCAGAGTGCC 
AACAAAGAAA AAAACTACAC TAGTATCAAA GACAAAGAGT CAGAGGAGAA TGCATCTGAA 
AAAAATGAAG ATGAAGATGA TGAATTACTT AAACCAGTCT GGGTTCGCTG TACCCATTCT 
GAAAGCTATT ATTCAAATGA CCCCATGGAT CAAGTGGGGG ACTCTACTGT GGTAGGCACA 
AGCAAGCTCC GAGATCTGTA TGAGAAGTTT GAGGAGGAGC TAGGAAAACG ACAGGCAAAG 
GCCAAAGCAG CCAGGCCACC ATGGGAGCCG CCGAAGACCA AGCTGGATGA AGATCTTGAG 
AGCTCCAGTG AGTCAGAATG CGACTCTGAT GATGACAGCA GCTGCTCCAG CAGTTCAGAT 
TCGGAAGTTT TTGATGTCAT TGCAGAAATT AAGCGTAAGA AAGCACACCC TGATCGTCTT 
CATGAGGAAC TGTGGTACAA TGATCCAGGA CAGATGAATG ATGGACCGCT GTGCAAGTGC 
AGCGCTAAAG CCCGACGAAC AGGGATAAGA CATGGCATTT ATCCTGGTGA AGAGCCCATT 
AAACCATGTC GCCCCATGAC CAACAATGCT GGAAGACTGT ACCATTATCG AATTACAGTG 
TCACCTCCCA CAAACTTCTT AACAGACAGA CCTACTGTTA TTGAGTATGA TGACCATGAA 
TATATCTTCG AAGGATTCTC CATGTTTTCA CATGCCCCAC TGACAAACAT TCCTCTATGT 
AAAGTAATCA GATTTAACAT TGACTATACA ATTCATTTCA TTGAAGAGAT GATGCCTGAG 
AATTTTTGTG TGAAAGGTCT TGAGCTGTTC TCTTCTTATC TATTCAAAGA TATTCTGGAG 
CTGTATGACT GGCATCTTAA AGGTCCTTCA GTTGAAAATG ATTCTGTTTC CTGCCCCAGA 
TTTCACTTCA TGCCGCGATT TGTGAGATTT CTTCCAGATG GAGGGAAGGA AGTACTGTCA 
ATGCATCAGA TTCTTCTCTA CTTGTTACGA TGCAATAAAG CTTTGGTGCC TGAAGAAGAG 
ATTGCCAACA TGCTTCAGTG GGAAGAGCTG GAATGGCAGA AATATGCAGA GGAATGCAAA 
GGGATGATCG TTACCAGCCC TGGCATGAAA CCCAGCTCAG TTCGTATTGA TCAACTGGAC 
CGTGAACAAT TCAATCCTGA TGTAATTACT TTCCCCATTA TTGTCCACTT TGGGATACGA 
CCTGCTCAAC TCAGCTACGC TGGAGATCCT CAATACCAAA AACTATGGAA GAGTTATGTG 
AAGCTGCGTC ACCTGCTAGC TAACAGTCCC AAAGTCAAAC AAGCTGATAA ACAGAAATTA 
TCACAGAGAG AGGAAGCACT GCAGAAAATC CGGCAGAAGA ACACGATGAG ACGTGAAGTG 
ACTGTCGAGT TGAGTAGCCA GGGATTCTGG AAGACGGGGA TACGCTCTGA TGTCTGCCAG 
CACGCAATGA TGCTTCCTGT CCTCACCCAC CATATTCGCT ACCATCAGTG TCTGATGCAT 
TTGGACAAGT TGATAGGCTA CACTTTTCGA GATAGGTGCC TGCTGCAGCT TGCCATGACT 
CATCCGAGCC ATCACTTAAA CTTTGGAATG AATCCAGACC ATGCCAGAAA TTCCTTATCC 
AACTGTGGGA TTCGACAGCC AAAGTATGGG GACAGAAAAG TTCACCATAT GCATATGAGA 
AAAAAAGGGA TAAACACCCT GATAAATATT ATGTCCCGTT TGGGACAGGA TGATCCAGCT 
CCTTCCAGGA TTAACCACAA TGAGCGTTTA GAATTTCTGG GAGATGCTGT GGTGGAGTTC 
TTAACAAGTG TCCACTTGTA CTACCTGTTT CCCACTCTGG AGGAAGGAGG ATTAGCTACG 
TACCGCACTG CCATAGTTCA GAACCAGCAT CTGGCCATGC TGGCTAAGAA GCTGGAACTA 
GATCGATTTA TGCTTTATGC TCATGGTCCG GACCTTTGCA GGGAATCGGA TCTCCGCCAT 
GCCATGGCCA ACTGCTTTGA AGCACTAATA GGAGCAGTTT ATCTGGAGGG TAGTCTTGAA 
GAAGCCAAAC AATTATTTGG ACGGTTGCTC TTCAATGACA AGGACCTGCG GGATGTATGG 
CTTAATTATC CACTACACCC ACTCCAACTG CAGGAGTCTA ATACTGACAG GCAACTCATT 
GAGACCTCTC CAGTTCTTCA AAAGCTTACG GAGTTTGAGG AGGCAATTGG AGTCATCTTT 
ACTCATGTTC GACTTCTAGC ACGTGCATTC ACTCTGAGGA CAGTAGGCTT TAACCACCTG 
ACTCTAGGCC ACAATCAGAG GATGGAATTC CTGGGTGACT CCATAATGCA GTTGGTAGCC 
ACAGAGTATT TATTCATACA TTTCCCCGAT CATCATGAAG GGCACTTAAC GCTGTTGAGA 
AGCTCTCTGG TGAACAACAG GACTCAGGCC AAAGTGGCAG AAGAGCTGGG AATGCAAGAG 
TTTGCCATCA CTAATGACAA GACCAAAAGA CCCGTAGCTC TTCGAACTAA AACTCTGGCA 
GATCTCTTGG AATCCTTTAT TGCTGCCCTG TACATTGATA AAGATTTGGA ATATGTTCAC 
ACTTTCATGA ATGTATGCTT TTTTCCACGA CTAAAGGAAT TCATTTTAAA TCAAGATTGG 
AATGACCCTA AATCTCAGCT TCAGCAGTGC TGCTTGACTC TCAGGACAGA AGGAAAGGAG 
CCAGACATTC CTCTTTACAA GACTCTGCAG ACAGTGGGAC CATCTCATGC CAGGACGTAC 
ACTGTAGCTG TTTATTTCAA AGGGGAAAGA ATAGGATGTG GTAAAGGCCC AAGTATTCAA 
CAAGCAGAAA TGGGAGCTGC AATGGATGCA CTTGAAAAAT ATAACTTTCC CCAGATGGCC 
CATCAGAAAC GGTTCATTGA ACGGAAGTAC AGACAAGAGT TGAAAGAAAT GAGGTGGGAA 
AGAGAGCATC AAGAGAGAGA AACAGATGAG ACTGAAGAAG CAAGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_015282183.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137669.1 Gallus gallus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa32168
Clone ID Related Accession (Same CDS sequence)	XM_015282183.1 , XM_015282183.2 , XM_025147270.1 , XM_015282182.1 , XM_015282182.2
Accession Version	XM_015282183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ribonuclease 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTCC ATTCTGGACG AGGATGTCCC CGTGGGCAGG GGGGACCAGG AGCAAGAACT 
TCAGCACAGA CGTATCGTCC TCAGAACCTG CGGCAGCTTC ACCCCCAGCA GCCTTCTGTA 
CAGTACCAGT ATGACCAGCA AGCCGCACCT GCAGCTACCT ATTCAAACCC TTCAGCCACC 
AGTTACATGC CTCCCAGGCC AGACTTTGTT CCATATCCCC CACCAGTTCC CCCTTCCACA 
CAAAATCCCA TCAACCAGTG TCCCATGAGG CCACCGTTTC CAAACCATCA GATGAGGCAG 
AGCTTCCCAG TGCCTCCATG CTTCCCTCCT GCTCCTCCAC CAGTGCCAGC CCCTAGCAAT 
GCTCCCGTGC CAGCAACTGC TACTGGGCAG AGCACCTTTC CATACATGAT GCCTCCTCCC 
GCAGTACCCC ATCCTCCCCC ACCACCAGTC ATACCACAGC AAGTCAACTA CCAGCCTGTA 
TATTCCACAG GGTATTCCCA GCAGTCATTC CCACCACCTA ACTTCAATAG CTATCAGCAC 
AACTCTGGCT CCTTTCAGAG CAGCACTACC AATAGCAGCA GTGGCAGCAG CCATTTTCGG 
CACACGGGTC AGTACCAGGG AGAAAAGTCA CAAAGTGATC GACGATCTCC GGAGAGGAGC 
AAGCACTACG ACGAGCACCG GCACCGTGAG CACAGTCACA TCCACGGTGA CAGGCATCGA 
TCCAATAACC ACGGGGATCG TCGGGATCGA GGCCGCAGTC CAGACAGGAG GAGGCAGGAA 
AGCAGTCGGC ACCGGACTGA ATATGACAGA GGAAGAATGT CGCCTCATCA TAGAAGTTAT 
GAGCGTAGCA GGGAGCGTGA GAGGGACAGG CATAGGCACC GTGACAGCAG AAGATCACCA 
TCACCGGACA GGTCCTACAA AAAAGATTAT AAAAGAGCAG GAAGCCGCTC TCCAAGTAGA 
GAAAGAAAGA GACCCCGATG GGAGGAGGAA AGAGAAAGGT GGTCTGAGAG CCAGAGTGCC 
AACAAAGAAA AAAACTACAC TAGTATCAAA GACAAAGAGT CAGAGGAGAA TGCATCTGAA 
AAAAATGAAG ATGAAGATGA TGAATTACTT AAACCAGTCT GGGTTCGCTG TACCCATTCT 
GAAAGCTATT ATTCAAATGA CCCCATGGAT CAAGTGGGGG ACTCTACTGT GGTAGGCACA 
AGCAAGCTCC GAGATCTGTA TGAGAAGTTT GAGGAGGAGC TAGGAAAACG ACAGGCAAAG 
GCCAAAGCAG CCAGGCCACC ATGGGAGCCG CCGAAGACCA AGCTGGATGA AGATCTTGAG 
AGCTCCAGTG AGTCAGAATG CGACTCTGAT GATGACAGCA GCTGCTCCAG CAGTTCAGAT 
TCGGAAGTTT TTGATGTCAT TGCAGAAATT AAGCGTAAGA AAGCACACCC TGATCGTCTT 
CATGAGGAAC TGTGGTACAA TGATCCAGGA CAGATGAATG ATGGACCGCT GTGCAAGTGC 
AGCGCTAAAG CCCGACGAAC AGGGATAAGA CATGGCATTT ATCCTGGTGA AGAGCCCATT 
AAACCATGTC GCCCCATGAC CAACAATGCT GGAAGACTGT ACCATTATCG AATTACAGTG 
TCACCTCCCA CAAACTTCTT AACAGACAGA CCTACTGTTA TTGAGTATGA TGACCATGAA 
TATATCTTCG AAGGATTCTC CATGTTTTCA CATGCCCCAC TGACAAACAT TCCTCTATGT 
AAAGTAATCA GATTTAACAT TGACTATACA ATTCATTTCA TTGAAGAGAT GATGCCTGAG 
AATTTTTGTG TGAAAGGTCT TGAGCTGTTC TCTTCTTATC TATTCAAAGA TATTCTGGAG 
CTGTATGACT GGCATCTTAA AGGTCCTTCA GTTGAAAATG ATTCTGTTTC CTGCCCCAGA 
TTTCACTTCA TGCCGCGATT TGTGAGATTT CTTCCAGATG GAGGGAAGGA AGTACTGTCA 
ATGCATCAGA TTCTTCTCTA CTTGTTACGA TGCAATAAAG CTTTGGTGCC TGAAGAAGAG 
ATTGCCAACA TGCTTCAGTG GGAAGAGCTG GAATGGCAGA AATATGCAGA GGAATGCAAA 
GGGATGATCG TTACCAGCCC TGGCATGAAA CCCAGCTCAG TTCGTATTGA TCAACTGGAC 
CGTGAACAAT TCAATCCTGA TGTAATTACT TTCCCCATTA TTGTCCACTT TGGGATACGA 
CCTGCTCAAC TCAGCTACGC TGGAGATCCT CAATACCAAA AACTATGGAA GAGTTATGTG 
AAGCTGCGTC ACCTGCTAGC TAACAGTCCC AAAGTCAAAC AAGCTGATAA ACAGAAATTA 
TCACAGAGAG AGGAAGCACT GCAGAAAATC CGGCAGAAGA ACACGATGAG ACGTGAAGTG 
ACTGTCGAGT TGAGTAGCCA GGGATTCTGG AAGACGGGGA TACGCTCTGA TGTCTGCCAG 
CACGCAATGA TGCTTCCTGT CCTCACCCAC CATATTCGCT ACCATCAGTG TCTGATGCAT 
TTGGACAAGT TGATAGGCTA CACTTTTCGA GATAGGTGCC TGCTGCAGCT TGCCATGACT 
CATCCGAGCC ATCACTTAAA CTTTGGAATG AATCCAGACC ATGCCAGAAA TTCCTTATCC 
AACTGTGGGA TTCGACAGCC AAAGTATGGG GACAGAAAAG TTCACCATAT GCATATGAGA 
AAAAAAGGGA TAAACACCCT GATAAATATT ATGTCCCGTT TGGGACAGGA TGATCCAGCT 
CCTTCCAGGA TTAACCACAA TGAGCGTTTA GAATTTCTGG GAGATGCTGT GGTGGAGTTC 
TTAACAAGTG TCCACTTGTA CTACCTGTTT CCCACTCTGG AGGAAGGAGG ATTAGCTACG 
TACCGCACTG CCATAGTTCA GAACCAGCAT CTGGCCATGC TGGCTAAGAA GCTGGAACTA 
GATCGATTTA TGCTTTATGC TCATGGTCCG GACCTTTGCA GGGAATCGGA TCTCCGCCAT 
GCCATGGCCA ACTGCTTTGA AGCACTAATA GGAGCAGTTT ATCTGGAGGG TAGTCTTGAA 
GAAGCCAAAC AATTATTTGG ACGGTTGCTC TTCAATGACA AGGACCTGCG GGATGTATGG 
CTTAATTATC CACTACACCC ACTCCAACTG CAGGAGTCTA ATACTGACAG GCAACTCATT 
GAGACCTCTC CAGTTCTTCA AAAGCTTACG GAGTTTGAGG AGGCAATTGG AGTCATCTTT 
ACTCATGTTC GACTTCTAGC ACGTGCATTC ACTCTGAGGA CAGTAGGCTT TAACCACCTG 
ACTCTAGGCC ACAATCAGAG GATGGAATTC CTGGGTGACT CCATAATGCA GTTGGTAGCC 
ACAGAGTATT TATTCATACA TTTCCCCGAT CATCATGAAG GGCACTTAAC GCTGTTGAGA 
AGCTCTCTGG TGAACAACAG GACTCAGGCC AAAGTGGCAG AAGAGCTGGG AATGCAAGAG 
TTTGCCATCA CTAATGACAA GACCAAAAGA CCCGTAGCTC TTCGAACTAA AACTCTGGCA 
GATCTCTTGG AATCCTTTAT TGCTGCCCTG TACATTGATA AAGATTTGGA ATATGTTCAC 
ACTTTCATGA ATGTATGCTT TTTTCCACGA CTAAAGGAAT TCATTTTAAA TCAAGATTGG 
AATGACCCTA AATCTCAGCT TCAGCAGTGC TGCTTGACTC TCAGGACAGA AGGAAAGGAG 
CCAGACATTC CTCTTTACAA GACTCTGCAG ACAGTGGGAC CATCTCATGC CAGGACGTAC 
ACTGTAGCTG TTTATTTCAA AGGGGAAAGA ATAGGATGTG GTAAAGGCCC AAGTATTCAA 
CAAGCAGAAA TGGGAGCTGC AATGGATGCA CTTGAAAAAT ATAACTTTCC CCAGATGGCC 
CATCAGAAAC GGTTCATTGA ACGGAAGTAC AGACAAGAGT TGAAAGAAAT GAGGTGGGAA 
AGAGAGCATC AAGAGAGAGA AACAGATGAG ACTGAAGAAG CAAGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137669.1
CDS	292..4302
Translation	MSFHSGRGCPRGQGGPGARTSAQTYRPQNLRQLHPQQPSVQYQYDQQAAPAATYSNPSATSYMPPRPDFVPYPPPVPPSTQNPINQCPMRPPFPNHQMRQSFPVPPCFPPAPPPVPAPSNAPVPATATGQSTFPYMMPPPAVPHPPPPPVIPQQVNYQPVYSTGYSQQSFPPPNFNSYQHNSGSFQSSTTNSSSGSSHFRHTGQYQGEKSQSDRRSPERSKHYDEHRHREHSHIHGDRHRSNNHGDRRDRGRSPDRRRQESSRHRTEYDRGRMSPHHRSYERSRERERDRHRHRDSRRSPSPDRSYKKDYKRAGSRSPSRERKRPRWEEERERWSESQSANKEKNYTSIKDKESEENASEKNEDEDDELLKPVWVRCTHSESYYSNDPMDQVGDSTVVGTSKLRDLYEKFEEELGKRQAKAKAARPPWEPPKTKLDEDLESSSESECDSDDDSSCSSSSDSEVFDVIAEIKRKKAHPDRLHEELWYNDPGQMNDGPLCKCSAKARRTGIRHGIYPGEEPIKPCRPMTNNAGRLYHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFSHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSSYLFKDILELYDWHLKGPSVENDSVSCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCNKALVPEEEIANMLQWEELEWQKYAEECKGMIVTSPGMKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQADKQKLSQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFRDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPAPSRINHNERLEFLGDAVVEFLTSVHLYYLFPTLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDKDLRDVWLNYPLHPLQLQESNTDRQLIETSPVLQKLTEFEEAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEFAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQERETDETEEARK

Target ORF information:

RefSeq Version	XM_015282183.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282183.2

ORF Insert Sequence:

ATGTCTTTCC ATTCTGGACG AGGATGTCCC CGTGGGCAGG GGGGACCAGG AGCAAGAACT 
TCAGCACAGA CGTATCGTCC TCAGAACCTG CGGCAGCTTC ACCCCCAGCA GCCTTCTGTA 
CAGTACCAGT ATGACCAGCA AGCCGCACCT GCAGCTACCT ATTCAAACCC TTCAGCCACC 
AGTTACATGC CTCCCAGGCC AGACTTTGTT CCATATCCCC CACCAGTTCC CCCTTCCACA 
CAAAATCCCA TCAACCAGTG TCCCATGAGG CCACCGTTTC CAAACCATCA GATGAGGCAG 
AGCTTCCCAG TGCCTCCATG CTTCCCTCCT GCTCCTCCAC CAGTGCCAGC CCCTAGCAAT 
GCTCCCGTGC CAGCAACTGC TACTGGGCAG AGCACCTTTC CATACATGAT GCCTCCTCCC 
GCAGTACCCC ATCCTCCCCC ACCACCAGTC ATACCACAGC AAGTCAACTA CCAGCCTGTA 
TATTCCACAG GGTATTCCCA GCAGTCATTC CCACCACCTA ACTTCAATAG CTATCAGCAC 
AACTCTGGCT CCTTTCAGAG CAGCACTACC AATAGCAGCA GTGGCAGCAG CCATTTTCGG 
CACACGGGTC AGTACCAGGG AGAAAAGTCA CAAAGTGATC GACGATCTCC GGAGAGGAGC 
AAGCACTACG ACGAGCACCG GCACCGTGAG CACAGTCACA TCCACGGTGA CAGGCATCGA 
TCCAATAACC ACGGGGATCG TCGGGATCGA GGCCGCAGTC CAGACAGGAG GAGGCAGGAA 
AGCAGTCGGC ACCGGACTGA ATATGACAGA GGAAGAATGT CGCCTCATCA TAGAAGTTAT 
GAGCGTAGCA GGGAGCGTGA GAGGGACAGG CATAGGCACC GTGACAGCAG AAGATCACCA 
TCACCGGACA GGTCCTACAA AAAAGATTAT AAAAGAGCAG GAAGCCGCTC TCCAAGTAGA 
GAAAGAAAGA GACCCCGATG GGAGGAGGAA AGAGAAAGGT GGTCTGAGAG CCAGAGTGCC 
AACAAAGAAA AAAACTACAC TAGTATCAAA GACAAAGAGT CAGAGGAGAA TGCATCTGAA 
AAAAATGAAG ATGAAGATGA TGAATTACTT AAACCAGTCT GGGTTCGCTG TACCCATTCT 
GAAAGCTATT ATTCAAATGA CCCCATGGAT CAAGTGGGGG ACTCTACTGT GGTAGGCACA 
AGCAAGCTCC GAGATCTGTA TGAGAAGTTT GAGGAGGAGC TAGGAAAACG ACAGGCAAAG 
GCCAAAGCAG CCAGGCCACC ATGGGAGCCG CCGAAGACCA AGCTGGATGA AGATCTTGAG 
AGCTCCAGTG AGTCAGAATG CGACTCTGAT GATGACAGCA GCTGCTCCAG CAGTTCAGAT 
TCGGAAGTTT TTGATGTCAT TGCAGAAATT AAGCGTAAGA AAGCACACCC TGATCGTCTT 
CATGAGGAAC TGTGGTACAA TGATCCAGGA CAGATGAATG ATGGACCGCT GTGCAAGTGC 
AGCGCTAAAG CCCGACGAAC AGGGATAAGA CATGGCATTT ATCCTGGTGA AGAGCCCATT 
AAACCATGTC GCCCCATGAC CAACAATGCT GGAAGACTGT ACCATTATCG AATTACAGTG 
TCACCTCCCA CAAACTTCTT AACAGACAGA CCTACTGTTA TTGAGTATGA TGACCATGAA 
TATATCTTCG AAGGATTCTC CATGTTTTCA CATGCCCCAC TGACAAACAT TCCTCTATGT 
AAAGTAATCA GATTTAACAT TGACTATACA ATTCATTTCA TTGAAGAGAT GATGCCTGAG 
AATTTTTGTG TGAAAGGTCT TGAGCTGTTC TCTTCTTATC TATTCAAAGA TATTCTGGAG 
CTGTATGACT GGCATCTTAA AGGTCCTTCA GTTGAAAATG ATTCTGTTTC CTGCCCCAGA 
TTTCACTTCA TGCCGCGATT TGTGAGATTT CTTCCAGATG GAGGGAAGGA AGTACTGTCA 
ATGCATCAGA TTCTTCTCTA CTTGTTACGA TGCAATAAAG CTTTGGTGCC TGAAGAAGAG 
ATTGCCAACA TGCTTCAGTG GGAAGAGCTG GAATGGCAGA AATATGCAGA GGAATGCAAA 
GGGATGATCG TTACCAGCCC TGGCATGAAA CCCAGCTCAG TTCGTATTGA TCAACTGGAC 
CGTGAACAAT TCAATCCTGA TGTAATTACT TTCCCCATTA TTGTCCACTT TGGGATACGA 
CCTGCTCAAC TCAGCTACGC TGGAGATCCT CAATACCAAA AACTATGGAA GAGTTATGTG 
AAGCTGCGTC ACCTGCTAGC TAACAGTCCC AAAGTCAAAC AAGCTGATAA ACAGAAATTA 
TCACAGAGAG AGGAAGCACT GCAGAAAATC CGGCAGAAGA ACACGATGAG ACGTGAAGTG 
ACTGTCGAGT TGAGTAGCCA GGGATTCTGG AAGACGGGGA TACGCTCTGA TGTCTGCCAG 
CACGCAATGA TGCTTCCTGT CCTCACCCAC CATATTCGCT ACCATCAGTG TCTGATGCAT 
TTGGACAAGT TGATAGGCTA CACTTTTCGA GATAGGTGCC TGCTGCAGCT TGCCATGACT 
CATCCGAGCC ATCACTTAAA CTTTGGAATG AATCCAGACC ATGCCAGAAA TTCCTTATCC 
AACTGTGGGA TTCGACAGCC AAAGTATGGG GACAGAAAAG TTCACCATAT GCATATGAGA 
AAAAAAGGGA TAAACACCCT GATAAATATT ATGTCCCGTT TGGGACAGGA TGATCCAGCT 
CCTTCCAGGA TTAACCACAA TGAGCGTTTA GAATTTCTGG GAGATGCTGT GGTGGAGTTC 
TTAACAAGTG TCCACTTGTA CTACCTGTTT CCCACTCTGG AGGAAGGAGG ATTAGCTACG 
TACCGCACTG CCATAGTTCA GAACCAGCAT CTGGCCATGC TGGCTAAGAA GCTGGAACTA 
GATCGATTTA TGCTTTATGC TCATGGTCCG GACCTTTGCA GGGAATCGGA TCTCCGCCAT 
GCCATGGCCA ACTGCTTTGA AGCACTAATA GGAGCAGTTT ATCTGGAGGG TAGTCTTGAA 
GAAGCCAAAC AATTATTTGG ACGGTTGCTC TTCAATGACA AGGACCTGCG GGATGTATGG 
CTTAATTATC CACTACACCC ACTCCAACTG CAGGAGTCTA ATACTGACAG GCAACTCATT 
GAGACCTCTC CAGTTCTTCA AAAGCTTACG GAGTTTGAGG AGGCAATTGG AGTCATCTTT 
ACTCATGTTC GACTTCTAGC ACGTGCATTC ACTCTGAGGA CAGTAGGCTT TAACCACCTG 
ACTCTAGGCC ACAATCAGAG GATGGAATTC CTGGGTGACT CCATAATGCA GTTGGTAGCC 
ACAGAGTATT TATTCATACA TTTCCCCGAT CATCATGAAG GGCACTTAAC GCTGTTGAGA 
AGCTCTCTGG TGAACAACAG GACTCAGGCC AAAGTGGCAG AAGAGCTGGG AATGCAAGAG 
TTTGCCATCA CTAATGACAA GACCAAAAGA CCCGTAGCTC TTCGAACTAA AACTCTGGCA 
GATCTCTTGG AATCCTTTAT TGCTGCCCTG TACATTGATA AAGATTTGGA ATATGTTCAC 
ACTTTCATGA ATGTATGCTT TTTTCCACGA CTAAAGGAAT TCATTTTAAA TCAAGATTGG 
AATGACCCTA AATCTCAGCT TCAGCAGTGC TGCTTGACTC TCAGGACAGA AGGAAAGGAG 
CCAGACATTC CTCTTTACAA GACTCTGCAG ACAGTGGGAC CATCTCATGC CAGGACGTAC 
ACTGTAGCTG TTTATTTCAA AGGGGAAAGA ATAGGATGTG GTAAAGGCCC AAGTATTCAA 
CAAGCAGAAA TGGGAGCTGC AATGGATGCA CTTGAAAAAT ATAACTTTCC CCAGATGGCC 
CATCAGAAAC GGTTCATTGA ACGGAAGTAC AGACAAGAGT TGAAAGAAAT GAGGTGGGAA 
AGAGAGCATC AAGAGAGAGA AACAGATGAG ACTGAAGAAG CAAGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_025147270.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025003038.1 Gallus gallus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.

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CloneID	OGa32168
Clone ID Related Accession (Same CDS sequence)	XM_015282183.1 , XM_015282183.2 , XM_025147270.1 , XM_015282182.1 , XM_015282182.2
Accession Version	XM_025147270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ribonuclease 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTCC ATTCTGGACG AGGATGTCCC CGTGGGCAGG GGGGACCAGG AGCAAGAACT 
TCAGCACAGA CGTATCGTCC TCAGAACCTG CGGCAGCTTC ACCCCCAGCA GCCTTCTGTA 
CAGTACCAGT ATGACCAGCA AGCCGCACCT GCAGCTACCT ATTCAAACCC TTCAGCCACC 
AGTTACATGC CTCCCAGGCC AGACTTTGTT CCATATCCCC CACCAGTTCC CCCTTCCACA 
CAAAATCCCA TCAACCAGTG TCCCATGAGG CCACCGTTTC CAAACCATCA GATGAGGCAG 
AGCTTCCCAG TGCCTCCATG CTTCCCTCCT GCTCCTCCAC CAGTGCCAGC CCCTAGCAAT 
GCTCCCGTGC CAGCAACTGC TACTGGGCAG AGCACCTTTC CATACATGAT GCCTCCTCCC 
GCAGTACCCC ATCCTCCCCC ACCACCAGTC ATACCACAGC AAGTCAACTA CCAGCCTGTA 
TATTCCACAG GGTATTCCCA GCAGTCATTC CCACCACCTA ACTTCAATAG CTATCAGCAC 
AACTCTGGCT CCTTTCAGAG CAGCACTACC AATAGCAGCA GTGGCAGCAG CCATTTTCGG 
CACACGGGTC AGTACCAGGG AGAAAAGTCA CAAAGTGATC GACGATCTCC GGAGAGGAGC 
AAGCACTACG ACGAGCACCG GCACCGTGAG CACAGTCACA TCCACGGTGA CAGGCATCGA 
TCCAATAACC ACGGGGATCG TCGGGATCGA GGCCGCAGTC CAGACAGGAG GAGGCAGGAA 
AGCAGTCGGC ACCGGACTGA ATATGACAGA GGAAGAATGT CGCCTCATCA TAGAAGTTAT 
GAGCGTAGCA GGGAGCGTGA GAGGGACAGG CATAGGCACC GTGACAGCAG AAGATCACCA 
TCACCGGACA GGTCCTACAA AAAAGATTAT AAAAGAGCAG GAAGCCGCTC TCCAAGTAGA 
GAAAGAAAGA GACCCCGATG GGAGGAGGAA AGAGAAAGGT GGTCTGAGAG CCAGAGTGCC 
AACAAAGAAA AAAACTACAC TAGTATCAAA GACAAAGAGT CAGAGGAGAA TGCATCTGAA 
AAAAATGAAG ATGAAGATGA TGAATTACTT AAACCAGTCT GGGTTCGCTG TACCCATTCT 
GAAAGCTATT ATTCAAATGA CCCCATGGAT CAAGTGGGGG ACTCTACTGT GGTAGGCACA 
AGCAAGCTCC GAGATCTGTA TGAGAAGTTT GAGGAGGAGC TAGGAAAACG ACAGGCAAAG 
GCCAAAGCAG CCAGGCCACC ATGGGAGCCG CCGAAGACCA AGCTGGATGA AGATCTTGAG 
AGCTCCAGTG AGTCAGAATG CGACTCTGAT GATGACAGCA GCTGCTCCAG CAGTTCAGAT 
TCGGAAGTTT TTGATGTCAT TGCAGAAATT AAGCGTAAGA AAGCACACCC TGATCGTCTT 
CATGAGGAAC TGTGGTACAA TGATCCAGGA CAGATGAATG ATGGACCGCT GTGCAAGTGC 
AGCGCTAAAG CCCGACGAAC AGGGATAAGA CATGGCATTT ATCCTGGTGA AGAGCCCATT 
AAACCATGTC GCCCCATGAC CAACAATGCT GGAAGACTGT ACCATTATCG AATTACAGTG 
TCACCTCCCA CAAACTTCTT AACAGACAGA CCTACTGTTA TTGAGTATGA TGACCATGAA 
TATATCTTCG AAGGATTCTC CATGTTTTCA CATGCCCCAC TGACAAACAT TCCTCTATGT 
AAAGTAATCA GATTTAACAT TGACTATACA ATTCATTTCA TTGAAGAGAT GATGCCTGAG 
AATTTTTGTG TGAAAGGTCT TGAGCTGTTC TCTTCTTATC TATTCAAAGA TATTCTGGAG 
CTGTATGACT GGCATCTTAA AGGTCCTTCA GTTGAAAATG ATTCTGTTTC CTGCCCCAGA 
TTTCACTTCA TGCCGCGATT TGTGAGATTT CTTCCAGATG GAGGGAAGGA AGTACTGTCA 
ATGCATCAGA TTCTTCTCTA CTTGTTACGA TGCAATAAAG CTTTGGTGCC TGAAGAAGAG 
ATTGCCAACA TGCTTCAGTG GGAAGAGCTG GAATGGCAGA AATATGCAGA GGAATGCAAA 
GGGATGATCG TTACCAGCCC TGGCATGAAA CCCAGCTCAG TTCGTATTGA TCAACTGGAC 
CGTGAACAAT TCAATCCTGA TGTAATTACT TTCCCCATTA TTGTCCACTT TGGGATACGA 
CCTGCTCAAC TCAGCTACGC TGGAGATCCT CAATACCAAA AACTATGGAA GAGTTATGTG 
AAGCTGCGTC ACCTGCTAGC TAACAGTCCC AAAGTCAAAC AAGCTGATAA ACAGAAATTA 
TCACAGAGAG AGGAAGCACT GCAGAAAATC CGGCAGAAGA ACACGATGAG ACGTGAAGTG 
ACTGTCGAGT TGAGTAGCCA GGGATTCTGG AAGACGGGGA TACGCTCTGA TGTCTGCCAG 
CACGCAATGA TGCTTCCTGT CCTCACCCAC CATATTCGCT ACCATCAGTG TCTGATGCAT 
TTGGACAAGT TGATAGGCTA CACTTTTCGA GATAGGTGCC TGCTGCAGCT TGCCATGACT 
CATCCGAGCC ATCACTTAAA CTTTGGAATG AATCCAGACC ATGCCAGAAA TTCCTTATCC 
AACTGTGGGA TTCGACAGCC AAAGTATGGG GACAGAAAAG TTCACCATAT GCATATGAGA 
AAAAAAGGGA TAAACACCCT GATAAATATT ATGTCCCGTT TGGGACAGGA TGATCCAGCT 
CCTTCCAGGA TTAACCACAA TGAGCGTTTA GAATTTCTGG GAGATGCTGT GGTGGAGTTC 
TTAACAAGTG TCCACTTGTA CTACCTGTTT CCCACTCTGG AGGAAGGAGG ATTAGCTACG 
TACCGCACTG CCATAGTTCA GAACCAGCAT CTGGCCATGC TGGCTAAGAA GCTGGAACTA 
GATCGATTTA TGCTTTATGC TCATGGTCCG GACCTTTGCA GGGAATCGGA TCTCCGCCAT 
GCCATGGCCA ACTGCTTTGA AGCACTAATA GGAGCAGTTT ATCTGGAGGG TAGTCTTGAA 
GAAGCCAAAC AATTATTTGG ACGGTTGCTC TTCAATGACA AGGACCTGCG GGATGTATGG 
CTTAATTATC CACTACACCC ACTCCAACTG CAGGAGTCTA ATACTGACAG GCAACTCATT 
GAGACCTCTC CAGTTCTTCA AAAGCTTACG GAGTTTGAGG AGGCAATTGG AGTCATCTTT 
ACTCATGTTC GACTTCTAGC ACGTGCATTC ACTCTGAGGA CAGTAGGCTT TAACCACCTG 
ACTCTAGGCC ACAATCAGAG GATGGAATTC CTGGGTGACT CCATAATGCA GTTGGTAGCC 
ACAGAGTATT TATTCATACA TTTCCCCGAT CATCATGAAG GGCACTTAAC GCTGTTGAGA 
AGCTCTCTGG TGAACAACAG GACTCAGGCC AAAGTGGCAG AAGAGCTGGG AATGCAAGAG 
TTTGCCATCA CTAATGACAA GACCAAAAGA CCCGTAGCTC TTCGAACTAA AACTCTGGCA 
GATCTCTTGG AATCCTTTAT TGCTGCCCTG TACATTGATA AAGATTTGGA ATATGTTCAC 
ACTTTCATGA ATGTATGCTT TTTTCCACGA CTAAAGGAAT TCATTTTAAA TCAAGATTGG 
AATGACCCTA AATCTCAGCT TCAGCAGTGC TGCTTGACTC TCAGGACAGA AGGAAAGGAG 
CCAGACATTC CTCTTTACAA GACTCTGCAG ACAGTGGGAC CATCTCATGC CAGGACGTAC 
ACTGTAGCTG TTTATTTCAA AGGGGAAAGA ATAGGATGTG GTAAAGGCCC AAGTATTCAA 
CAAGCAGAAA TGGGAGCTGC AATGGATGCA CTTGAAAAAT ATAACTTTCC CCAGATGGCC 
CATCAGAAAC GGTTCATTGA ACGGAAGTAC AGACAAGAGT TGAAAGAAAT GAGGTGGGAA 
AGAGAGCATC AAGAGAGAGA AACAGATGAG ACTGAAGAAG CAAGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025003038.1
CDS	273..4283
Translation	MSFHSGRGCPRGQGGPGARTSAQTYRPQNLRQLHPQQPSVQYQYDQQAAPAATYSNPSATSYMPPRPDFVPYPPPVPPSTQNPINQCPMRPPFPNHQMRQSFPVPPCFPPAPPPVPAPSNAPVPATATGQSTFPYMMPPPAVPHPPPPPVIPQQVNYQPVYSTGYSQQSFPPPNFNSYQHNSGSFQSSTTNSSSGSSHFRHTGQYQGEKSQSDRRSPERSKHYDEHRHREHSHIHGDRHRSNNHGDRRDRGRSPDRRRQESSRHRTEYDRGRMSPHHRSYERSRERERDRHRHRDSRRSPSPDRSYKKDYKRAGSRSPSRERKRPRWEEERERWSESQSANKEKNYTSIKDKESEENASEKNEDEDDELLKPVWVRCTHSESYYSNDPMDQVGDSTVVGTSKLRDLYEKFEEELGKRQAKAKAARPPWEPPKTKLDEDLESSSESECDSDDDSSCSSSSDSEVFDVIAEIKRKKAHPDRLHEELWYNDPGQMNDGPLCKCSAKARRTGIRHGIYPGEEPIKPCRPMTNNAGRLYHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFSHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSSYLFKDILELYDWHLKGPSVENDSVSCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCNKALVPEEEIANMLQWEELEWQKYAEECKGMIVTSPGMKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQADKQKLSQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFRDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPAPSRINHNERLEFLGDAVVEFLTSVHLYYLFPTLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDKDLRDVWLNYPLHPLQLQESNTDRQLIETSPVLQKLTEFEEAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEFAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQERETDETEEARK

Target ORF information:

RefSeq Version	XM_025147270.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025147270.1

ORF Insert Sequence:

ATGTCTTTCC ATTCTGGACG AGGATGTCCC CGTGGGCAGG GGGGACCAGG AGCAAGAACT 
TCAGCACAGA CGTATCGTCC TCAGAACCTG CGGCAGCTTC ACCCCCAGCA GCCTTCTGTA 
CAGTACCAGT ATGACCAGCA AGCCGCACCT GCAGCTACCT ATTCAAACCC TTCAGCCACC 
AGTTACATGC CTCCCAGGCC AGACTTTGTT CCATATCCCC CACCAGTTCC CCCTTCCACA 
CAAAATCCCA TCAACCAGTG TCCCATGAGG CCACCGTTTC CAAACCATCA GATGAGGCAG 
AGCTTCCCAG TGCCTCCATG CTTCCCTCCT GCTCCTCCAC CAGTGCCAGC CCCTAGCAAT 
GCTCCCGTGC CAGCAACTGC TACTGGGCAG AGCACCTTTC CATACATGAT GCCTCCTCCC 
GCAGTACCCC ATCCTCCCCC ACCACCAGTC ATACCACAGC AAGTCAACTA CCAGCCTGTA 
TATTCCACAG GGTATTCCCA GCAGTCATTC CCACCACCTA ACTTCAATAG CTATCAGCAC 
AACTCTGGCT CCTTTCAGAG CAGCACTACC AATAGCAGCA GTGGCAGCAG CCATTTTCGG 
CACACGGGTC AGTACCAGGG AGAAAAGTCA CAAAGTGATC GACGATCTCC GGAGAGGAGC 
AAGCACTACG ACGAGCACCG GCACCGTGAG CACAGTCACA TCCACGGTGA CAGGCATCGA 
TCCAATAACC ACGGGGATCG TCGGGATCGA GGCCGCAGTC CAGACAGGAG GAGGCAGGAA 
AGCAGTCGGC ACCGGACTGA ATATGACAGA GGAAGAATGT CGCCTCATCA TAGAAGTTAT 
GAGCGTAGCA GGGAGCGTGA GAGGGACAGG CATAGGCACC GTGACAGCAG AAGATCACCA 
TCACCGGACA GGTCCTACAA AAAAGATTAT AAAAGAGCAG GAAGCCGCTC TCCAAGTAGA 
GAAAGAAAGA GACCCCGATG GGAGGAGGAA AGAGAAAGGT GGTCTGAGAG CCAGAGTGCC 
AACAAAGAAA AAAACTACAC TAGTATCAAA GACAAAGAGT CAGAGGAGAA TGCATCTGAA 
AAAAATGAAG ATGAAGATGA TGAATTACTT AAACCAGTCT GGGTTCGCTG TACCCATTCT 
GAAAGCTATT ATTCAAATGA CCCCATGGAT CAAGTGGGGG ACTCTACTGT GGTAGGCACA 
AGCAAGCTCC GAGATCTGTA TGAGAAGTTT GAGGAGGAGC TAGGAAAACG ACAGGCAAAG 
GCCAAAGCAG CCAGGCCACC ATGGGAGCCG CCGAAGACCA AGCTGGATGA AGATCTTGAG 
AGCTCCAGTG AGTCAGAATG CGACTCTGAT GATGACAGCA GCTGCTCCAG CAGTTCAGAT 
TCGGAAGTTT TTGATGTCAT TGCAGAAATT AAGCGTAAGA AAGCACACCC TGATCGTCTT 
CATGAGGAAC TGTGGTACAA TGATCCAGGA CAGATGAATG ATGGACCGCT GTGCAAGTGC 
AGCGCTAAAG CCCGACGAAC AGGGATAAGA CATGGCATTT ATCCTGGTGA AGAGCCCATT 
AAACCATGTC GCCCCATGAC CAACAATGCT GGAAGACTGT ACCATTATCG AATTACAGTG 
TCACCTCCCA CAAACTTCTT AACAGACAGA CCTACTGTTA TTGAGTATGA TGACCATGAA 
TATATCTTCG AAGGATTCTC CATGTTTTCA CATGCCCCAC TGACAAACAT TCCTCTATGT 
AAAGTAATCA GATTTAACAT TGACTATACA ATTCATTTCA TTGAAGAGAT GATGCCTGAG 
AATTTTTGTG TGAAAGGTCT TGAGCTGTTC TCTTCTTATC TATTCAAAGA TATTCTGGAG 
CTGTATGACT GGCATCTTAA AGGTCCTTCA GTTGAAAATG ATTCTGTTTC CTGCCCCAGA 
TTTCACTTCA TGCCGCGATT TGTGAGATTT CTTCCAGATG GAGGGAAGGA AGTACTGTCA 
ATGCATCAGA TTCTTCTCTA CTTGTTACGA TGCAATAAAG CTTTGGTGCC TGAAGAAGAG 
ATTGCCAACA TGCTTCAGTG GGAAGAGCTG GAATGGCAGA AATATGCAGA GGAATGCAAA 
GGGATGATCG TTACCAGCCC TGGCATGAAA CCCAGCTCAG TTCGTATTGA TCAACTGGAC 
CGTGAACAAT TCAATCCTGA TGTAATTACT TTCCCCATTA TTGTCCACTT TGGGATACGA 
CCTGCTCAAC TCAGCTACGC TGGAGATCCT CAATACCAAA AACTATGGAA GAGTTATGTG 
AAGCTGCGTC ACCTGCTAGC TAACAGTCCC AAAGTCAAAC AAGCTGATAA ACAGAAATTA 
TCACAGAGAG AGGAAGCACT GCAGAAAATC CGGCAGAAGA ACACGATGAG ACGTGAAGTG 
ACTGTCGAGT TGAGTAGCCA GGGATTCTGG AAGACGGGGA TACGCTCTGA TGTCTGCCAG 
CACGCAATGA TGCTTCCTGT CCTCACCCAC CATATTCGCT ACCATCAGTG TCTGATGCAT 
TTGGACAAGT TGATAGGCTA CACTTTTCGA GATAGGTGCC TGCTGCAGCT TGCCATGACT 
CATCCGAGCC ATCACTTAAA CTTTGGAATG AATCCAGACC ATGCCAGAAA TTCCTTATCC 
AACTGTGGGA TTCGACAGCC AAAGTATGGG GACAGAAAAG TTCACCATAT GCATATGAGA 
AAAAAAGGGA TAAACACCCT GATAAATATT ATGTCCCGTT TGGGACAGGA TGATCCAGCT 
CCTTCCAGGA TTAACCACAA TGAGCGTTTA GAATTTCTGG GAGATGCTGT GGTGGAGTTC 
TTAACAAGTG TCCACTTGTA CTACCTGTTT CCCACTCTGG AGGAAGGAGG ATTAGCTACG 
TACCGCACTG CCATAGTTCA GAACCAGCAT CTGGCCATGC TGGCTAAGAA GCTGGAACTA 
GATCGATTTA TGCTTTATGC TCATGGTCCG GACCTTTGCA GGGAATCGGA TCTCCGCCAT 
GCCATGGCCA ACTGCTTTGA AGCACTAATA GGAGCAGTTT ATCTGGAGGG TAGTCTTGAA 
GAAGCCAAAC AATTATTTGG ACGGTTGCTC TTCAATGACA AGGACCTGCG GGATGTATGG 
CTTAATTATC CACTACACCC ACTCCAACTG CAGGAGTCTA ATACTGACAG GCAACTCATT 
GAGACCTCTC CAGTTCTTCA AAAGCTTACG GAGTTTGAGG AGGCAATTGG AGTCATCTTT 
ACTCATGTTC GACTTCTAGC ACGTGCATTC ACTCTGAGGA CAGTAGGCTT TAACCACCTG 
ACTCTAGGCC ACAATCAGAG GATGGAATTC CTGGGTGACT CCATAATGCA GTTGGTAGCC 
ACAGAGTATT TATTCATACA TTTCCCCGAT CATCATGAAG GGCACTTAAC GCTGTTGAGA 
AGCTCTCTGG TGAACAACAG GACTCAGGCC AAAGTGGCAG AAGAGCTGGG AATGCAAGAG 
TTTGCCATCA CTAATGACAA GACCAAAAGA CCCGTAGCTC TTCGAACTAA AACTCTGGCA 
GATCTCTTGG AATCCTTTAT TGCTGCCCTG TACATTGATA AAGATTTGGA ATATGTTCAC 
ACTTTCATGA ATGTATGCTT TTTTCCACGA CTAAAGGAAT TCATTTTAAA TCAAGATTGG 
AATGACCCTA AATCTCAGCT TCAGCAGTGC TGCTTGACTC TCAGGACAGA AGGAAAGGAG 
CCAGACATTC CTCTTTACAA GACTCTGCAG ACAGTGGGAC CATCTCATGC CAGGACGTAC 
ACTGTAGCTG TTTATTTCAA AGGGGAAAGA ATAGGATGTG GTAAAGGCCC AAGTATTCAA 
CAAGCAGAAA TGGGAGCTGC AATGGATGCA CTTGAAAAAT ATAACTTTCC CCAGATGGCC 
CATCAGAAAC GGTTCATTGA ACGGAAGTAC AGACAAGAGT TGAAAGAAAT GAGGTGGGAA 
AGAGAGCATC AAGAGAGAGA AACAGATGAG ACTGAAGAAG CAAGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_015282182.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137668.1 Gallus gallus drosha, ribonuclease type III (DROSHA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa32168
Clone ID Related Accession (Same CDS sequence)	XM_015282183.1 , XM_015282183.2 , XM_025147270.1 , XM_015282182.1 , XM_015282182.2
Accession Version	XM_015282182.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ribonuclease 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455777.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTCC ATTCTGGACG AGGATGTCCC CGTGGGCAGG GGGGACCAGG AGCAAGAACT 
TCAGCACAGA CGTATCGTCC TCAGAACCTG CGGCAGCTTC ACCCCCAGCA GCCTTCTGTA 
CAGTACCAGT ATGACCAGCA AGCCGCACCT GCAGCTACCT ATTCAAACCC TTCAGCCACC 
AGTTACATGC CTCCCAGGCC AGACTTTGTT CCATATCCCC CACCAGTTCC CCCTTCCACA 
CAAAATCCCA TCAACCAGTG TCCCATGAGG CCACCGTTTC CAAACCATCA GATGAGGCAG 
AGCTTCCCAG TGCCTCCATG CTTCCCTCCT GCTCCTCCAC CAGTGCCAGC CCCTAGCAAT 
GCTCCCGTGC CAGCAACTGC TACTGGGCAG AGCACCTTTC CATACATGAT GCCTCCTCCC 
GCAGTACCCC ATCCTCCCCC ACCACCAGTC ATACCACAGC AAGTCAACTA CCAGCCTGTA 
TATTCCACAG GGTATTCCCA GCAGTCATTC CCACCACCTA ACTTCAATAG CTATCAGCAC 
AACTCTGGCT CCTTTCAGAG CAGCACTACC AATAGCAGCA GTGGCAGCAG CCATTTTCGG 
CACACGGGTC AGTACCAGGG AGAAAAGTCA CAAAGTGATC GACGATCTCC GGAGAGGAGC 
AAGCACTACG ACGAGCACCG GCACCGTGAG CACAGTCACA TCCACGGTGA CAGGCATCGA 
TCCAATAACC ACGGGGATCG TCGGGATCGA GGCCGCAGTC CAGACAGGAG GAGGCAGGAA 
AGCAGTCGGC ACCGGACTGA ATATGACAGA GGAAGAATGT CGCCTCATCA TAGAAGTTAT 
GAGCGTAGCA GGGAGCGTGA GAGGGACAGG CATAGGCACC GTGACAGCAG AAGATCACCA 
TCACCGGACA GGTCCTACAA AAAAGATTAT AAAAGAGCAG GAAGCCGCTC TCCAAGTAGA 
GAAAGAAAGA GACCCCGATG GGAGGAGGAA AGAGAAAGGT GGTCTGAGAG CCAGAGTGCC 
AACAAAGAAA AAAACTACAC TAGTATCAAA GACAAAGAGT CAGAGGAGAA TGCATCTGAA 
AAAAATGAAG ATGAAGATGA TGAATTACTT AAACCAGTCT GGGTTCGCTG TACCCATTCT 
GAAAGCTATT ATTCAAATGA CCCCATGGAT CAAGTGGGGG ACTCTACTGT GGTAGGCACA 
AGCAAGCTCC GAGATCTGTA TGAGAAGTTT GAGGAGGAGC TAGGAAAACG ACAGGCAAAG 
GCCAAAGCAG CCAGGCCACC ATGGGAGCCG CCGAAGACCA AGCTGGATGA AGATCTTGAG 
AGCTCCAGTG AGTCAGAATG CGACTCTGAT GATGACAGCA GCTGCTCCAG CAGTTCAGAT 
TCGGAAGTTT TTGATGTCAT TGCAGAAATT AAGCGTAAGA AAGCACACCC TGATCGTCTT 
CATGAGGAAC TGTGGTACAA TGATCCAGGA CAGATGAATG ATGGACCGCT GTGCAAGTGC 
AGCGCTAAAG CCCGACGAAC AGGGATAAGA CATGGCATTT ATCCTGGTGA AGAGCCCATT 
AAACCATGTC GCCCCATGAC CAACAATGCT GGAAGACTGT ACCATTATCG AATTACAGTG 
TCACCTCCCA CAAACTTCTT AACAGACAGA CCTACTGTTA TTGAGTATGA TGACCATGAA 
TATATCTTCG AAGGATTCTC CATGTTTTCA CATGCCCCAC TGACAAACAT TCCTCTATGT 
AAAGTAATCA GATTTAACAT TGACTATACA ATTCATTTCA TTGAAGAGAT GATGCCTGAG 
AATTTTTGTG TGAAAGGTCT TGAGCTGTTC TCTTCTTATC TATTCAAAGA TATTCTGGAG 
CTGTATGACT GGCATCTTAA AGGTCCTTCA GTTGAAAATG ATTCTGTTTC CTGCCCCAGA 
TTTCACTTCA TGCCGCGATT TGTGAGATTT CTTCCAGATG GAGGGAAGGA AGTACTGTCA 
ATGCATCAGA TTCTTCTCTA CTTGTTACGA TGCAATAAAG CTTTGGTGCC TGAAGAAGAG 
ATTGCCAACA TGCTTCAGTG GGAAGAGCTG GAATGGCAGA AATATGCAGA GGAATGCAAA 
GGGATGATCG TTACCAGCCC TGGCATGAAA CCCAGCTCAG TTCGTATTGA TCAACTGGAC 
CGTGAACAAT TCAATCCTGA TGTAATTACT TTCCCCATTA TTGTCCACTT TGGGATACGA 
CCTGCTCAAC TCAGCTACGC TGGAGATCCT CAATACCAAA AACTATGGAA GAGTTATGTG 
AAGCTGCGTC ACCTGCTAGC TAACAGTCCC AAAGTCAAAC AAGCTGATAA ACAGAAATTA 
TCACAGAGAG AGGAAGCACT GCAGAAAATC CGGCAGAAGA ACACGATGAG ACGTGAAGTG 
ACTGTCGAGT TGAGTAGCCA GGGATTCTGG AAGACGGGGA TACGCTCTGA TGTCTGCCAG 
CACGCAATGA TGCTTCCTGT CCTCACCCAC CATATTCGCT ACCATCAGTG TCTGATGCAT 
TTGGACAAGT TGATAGGCTA CACTTTTCGA GATAGGTGCC TGCTGCAGCT TGCCATGACT 
CATCCGAGCC ATCACTTAAA CTTTGGAATG AATCCAGACC ATGCCAGAAA TTCCTTATCC 
AACTGTGGGA TTCGACAGCC AAAGTATGGG GACAGAAAAG TTCACCATAT GCATATGAGA 
AAAAAAGGGA TAAACACCCT GATAAATATT ATGTCCCGTT TGGGACAGGA TGATCCAGCT 
CCTTCCAGGA TTAACCACAA TGAGCGTTTA GAATTTCTGG GAGATGCTGT GGTGGAGTTC 
TTAACAAGTG TCCACTTGTA CTACCTGTTT CCCACTCTGG AGGAAGGAGG ATTAGCTACG 
TACCGCACTG CCATAGTTCA GAACCAGCAT CTGGCCATGC TGGCTAAGAA GCTGGAACTA 
GATCGATTTA TGCTTTATGC TCATGGTCCG GACCTTTGCA GGGAATCGGA TCTCCGCCAT 
GCCATGGCCA ACTGCTTTGA AGCACTAATA GGAGCAGTTT ATCTGGAGGG TAGTCTTGAA 
GAAGCCAAAC AATTATTTGG ACGGTTGCTC TTCAATGACA AGGACCTGCG GGATGTATGG 
CTTAATTATC CACTACACCC ACTCCAACTG CAGGAGTCTA ATACTGACAG GCAACTCATT 
GAGACCTCTC CAGTTCTTCA AAAGCTTACG GAGTTTGAGG AGGCAATTGG AGTCATCTTT 
ACTCATGTTC GACTTCTAGC ACGTGCATTC ACTCTGAGGA CAGTAGGCTT TAACCACCTG 
ACTCTAGGCC ACAATCAGAG GATGGAATTC CTGGGTGACT CCATAATGCA GTTGGTAGCC 
ACAGAGTATT TATTCATACA TTTCCCCGAT CATCATGAAG GGCACTTAAC GCTGTTGAGA 
AGCTCTCTGG TGAACAACAG GACTCAGGCC AAAGTGGCAG AAGAGCTGGG AATGCAAGAG 
TTTGCCATCA CTAATGACAA GACCAAAAGA CCCGTAGCTC TTCGAACTAA AACTCTGGCA 
GATCTCTTGG AATCCTTTAT TGCTGCCCTG TACATTGATA AAGATTTGGA ATATGTTCAC 
ACTTTCATGA ATGTATGCTT TTTTCCACGA CTAAAGGAAT TCATTTTAAA TCAAGATTGG 
AATGACCCTA AATCTCAGCT TCAGCAGTGC TGCTTGACTC TCAGGACAGA AGGAAAGGAG 
CCAGACATTC CTCTTTACAA GACTCTGCAG ACAGTGGGAC CATCTCATGC CAGGACGTAC 
ACTGTAGCTG TTTATTTCAA AGGGGAAAGA ATAGGATGTG GTAAAGGCCC AAGTATTCAA 
CAAGCAGAAA TGGGAGCTGC AATGGATGCA CTTGAAAAAT ATAACTTTCC CCAGATGGCC 
CATCAGAAAC GGTTCATTGA ACGGAAGTAC AGACAAGAGT TGAAAGAAAT GAGGTGGGAA 
AGAGAGCATC AAGAGAGAGA AACAGATGAG ACTGAAGAAG CAAGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137668.1
CDS	272..4282
Translation	MSFHSGRGCPRGQGGPGARTSAQTYRPQNLRQLHPQQPSVQYQYDQQAAPAATYSNPSATSYMPPRPDFVPYPPPVPPSTQNPINQCPMRPPFPNHQMRQSFPVPPCFPPAPPPVPAPSNAPVPATATGQSTFPYMMPPPAVPHPPPPPVIPQQVNYQPVYSTGYSQQSFPPPNFNSYQHNSGSFQSSTTNSSSGSSHFRHTGQYQGEKSQSDRRSPERSKHYDEHRHREHSHIHGDRHRSNNHGDRRDRGRSPDRRRQESSRHRTEYDRGRMSPHHRSYERSRERERDRHRHRDSRRSPSPDRSYKKDYKRAGSRSPSRERKRPRWEEERERWSESQSANKEKNYTSIKDKESEENASEKNEDEDDELLKPVWVRCTHSESYYSNDPMDQVGDSTVVGTSKLRDLYEKFEEELGKRQAKAKAARPPWEPPKTKLDEDLESSSESECDSDDDSSCSSSSDSEVFDVIAEIKRKKAHPDRLHEELWYNDPGQMNDGPLCKCSAKARRTGIRHGIYPGEEPIKPCRPMTNNAGRLYHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFSHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSSYLFKDILELYDWHLKGPSVENDSVSCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCNKALVPEEEIANMLQWEELEWQKYAEECKGMIVTSPGMKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQADKQKLSQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFRDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPAPSRINHNERLEFLGDAVVEFLTSVHLYYLFPTLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDKDLRDVWLNYPLHPLQLQESNTDRQLIETSPVLQKLTEFEEAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEFAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQERETDETEEARK

Target ORF information:

RefSeq Version	XM_015282182.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus drosha, ribonuclease type III (DROSHA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282182.1

ORF Insert Sequence:

ATGTCTTTCC ATTCTGGACG AGGATGTCCC CGTGGGCAGG GGGGACCAGG AGCAAGAACT 
TCAGCACAGA CGTATCGTCC TCAGAACCTG CGGCAGCTTC ACCCCCAGCA GCCTTCTGTA 
CAGTACCAGT ATGACCAGCA AGCCGCACCT GCAGCTACCT ATTCAAACCC TTCAGCCACC 
AGTTACATGC CTCCCAGGCC AGACTTTGTT CCATATCCCC CACCAGTTCC CCCTTCCACA 
CAAAATCCCA TCAACCAGTG TCCCATGAGG CCACCGTTTC CAAACCATCA GATGAGGCAG 
AGCTTCCCAG TGCCTCCATG CTTCCCTCCT GCTCCTCCAC CAGTGCCAGC CCCTAGCAAT 
GCTCCCGTGC CAGCAACTGC TACTGGGCAG AGCACCTTTC CATACATGAT GCCTCCTCCC 
GCAGTACCCC ATCCTCCCCC ACCACCAGTC ATACCACAGC AAGTCAACTA CCAGCCTGTA 
TATTCCACAG GGTATTCCCA GCAGTCATTC CCACCACCTA ACTTCAATAG CTATCAGCAC 
AACTCTGGCT CCTTTCAGAG CAGCACTACC AATAGCAGCA GTGGCAGCAG CCATTTTCGG 
CACACGGGTC AGTACCAGGG AGAAAAGTCA CAAAGTGATC GACGATCTCC GGAGAGGAGC 
AAGCACTACG ACGAGCACCG GCACCGTGAG CACAGTCACA TCCACGGTGA CAGGCATCGA 
TCCAATAACC ACGGGGATCG TCGGGATCGA GGCCGCAGTC CAGACAGGAG GAGGCAGGAA 
AGCAGTCGGC ACCGGACTGA ATATGACAGA GGAAGAATGT CGCCTCATCA TAGAAGTTAT 
GAGCGTAGCA GGGAGCGTGA GAGGGACAGG CATAGGCACC GTGACAGCAG AAGATCACCA 
TCACCGGACA GGTCCTACAA AAAAGATTAT AAAAGAGCAG GAAGCCGCTC TCCAAGTAGA 
GAAAGAAAGA GACCCCGATG GGAGGAGGAA AGAGAAAGGT GGTCTGAGAG CCAGAGTGCC 
AACAAAGAAA AAAACTACAC TAGTATCAAA GACAAAGAGT CAGAGGAGAA TGCATCTGAA 
AAAAATGAAG ATGAAGATGA TGAATTACTT AAACCAGTCT GGGTTCGCTG TACCCATTCT 
GAAAGCTATT ATTCAAATGA CCCCATGGAT CAAGTGGGGG ACTCTACTGT GGTAGGCACA 
AGCAAGCTCC GAGATCTGTA TGAGAAGTTT GAGGAGGAGC TAGGAAAACG ACAGGCAAAG 
GCCAAAGCAG CCAGGCCACC ATGGGAGCCG CCGAAGACCA AGCTGGATGA AGATCTTGAG 
AGCTCCAGTG AGTCAGAATG CGACTCTGAT GATGACAGCA GCTGCTCCAG CAGTTCAGAT 
TCGGAAGTTT TTGATGTCAT TGCAGAAATT AAGCGTAAGA AAGCACACCC TGATCGTCTT 
CATGAGGAAC TGTGGTACAA TGATCCAGGA CAGATGAATG ATGGACCGCT GTGCAAGTGC 
AGCGCTAAAG CCCGACGAAC AGGGATAAGA CATGGCATTT ATCCTGGTGA AGAGCCCATT 
AAACCATGTC GCCCCATGAC CAACAATGCT GGAAGACTGT ACCATTATCG AATTACAGTG 
TCACCTCCCA CAAACTTCTT AACAGACAGA CCTACTGTTA TTGAGTATGA TGACCATGAA 
TATATCTTCG AAGGATTCTC CATGTTTTCA CATGCCCCAC TGACAAACAT TCCTCTATGT 
AAAGTAATCA GATTTAACAT TGACTATACA ATTCATTTCA TTGAAGAGAT GATGCCTGAG 
AATTTTTGTG TGAAAGGTCT TGAGCTGTTC TCTTCTTATC TATTCAAAGA TATTCTGGAG 
CTGTATGACT GGCATCTTAA AGGTCCTTCA GTTGAAAATG ATTCTGTTTC CTGCCCCAGA 
TTTCACTTCA TGCCGCGATT TGTGAGATTT CTTCCAGATG GAGGGAAGGA AGTACTGTCA 
ATGCATCAGA TTCTTCTCTA CTTGTTACGA TGCAATAAAG CTTTGGTGCC TGAAGAAGAG 
ATTGCCAACA TGCTTCAGTG GGAAGAGCTG GAATGGCAGA AATATGCAGA GGAATGCAAA 
GGGATGATCG TTACCAGCCC TGGCATGAAA CCCAGCTCAG TTCGTATTGA TCAACTGGAC 
CGTGAACAAT TCAATCCTGA TGTAATTACT TTCCCCATTA TTGTCCACTT TGGGATACGA 
CCTGCTCAAC TCAGCTACGC TGGAGATCCT CAATACCAAA AACTATGGAA GAGTTATGTG 
AAGCTGCGTC ACCTGCTAGC TAACAGTCCC AAAGTCAAAC AAGCTGATAA ACAGAAATTA 
TCACAGAGAG AGGAAGCACT GCAGAAAATC CGGCAGAAGA ACACGATGAG ACGTGAAGTG 
ACTGTCGAGT TGAGTAGCCA GGGATTCTGG AAGACGGGGA TACGCTCTGA TGTCTGCCAG 
CACGCAATGA TGCTTCCTGT CCTCACCCAC CATATTCGCT ACCATCAGTG TCTGATGCAT 
TTGGACAAGT TGATAGGCTA CACTTTTCGA GATAGGTGCC TGCTGCAGCT TGCCATGACT 
CATCCGAGCC ATCACTTAAA CTTTGGAATG AATCCAGACC ATGCCAGAAA TTCCTTATCC 
AACTGTGGGA TTCGACAGCC AAAGTATGGG GACAGAAAAG TTCACCATAT GCATATGAGA 
AAAAAAGGGA TAAACACCCT GATAAATATT ATGTCCCGTT TGGGACAGGA TGATCCAGCT 
CCTTCCAGGA TTAACCACAA TGAGCGTTTA GAATTTCTGG GAGATGCTGT GGTGGAGTTC 
TTAACAAGTG TCCACTTGTA CTACCTGTTT CCCACTCTGG AGGAAGGAGG ATTAGCTACG 
TACCGCACTG CCATAGTTCA GAACCAGCAT CTGGCCATGC TGGCTAAGAA GCTGGAACTA 
GATCGATTTA TGCTTTATGC TCATGGTCCG GACCTTTGCA GGGAATCGGA TCTCCGCCAT 
GCCATGGCCA ACTGCTTTGA AGCACTAATA GGAGCAGTTT ATCTGGAGGG TAGTCTTGAA 
GAAGCCAAAC AATTATTTGG ACGGTTGCTC TTCAATGACA AGGACCTGCG GGATGTATGG 
CTTAATTATC CACTACACCC ACTCCAACTG CAGGAGTCTA ATACTGACAG GCAACTCATT 
GAGACCTCTC CAGTTCTTCA AAAGCTTACG GAGTTTGAGG AGGCAATTGG AGTCATCTTT 
ACTCATGTTC GACTTCTAGC ACGTGCATTC ACTCTGAGGA CAGTAGGCTT TAACCACCTG 
ACTCTAGGCC ACAATCAGAG GATGGAATTC CTGGGTGACT CCATAATGCA GTTGGTAGCC 
ACAGAGTATT TATTCATACA TTTCCCCGAT CATCATGAAG GGCACTTAAC GCTGTTGAGA 
AGCTCTCTGG TGAACAACAG GACTCAGGCC AAAGTGGCAG AAGAGCTGGG AATGCAAGAG 
TTTGCCATCA CTAATGACAA GACCAAAAGA CCCGTAGCTC TTCGAACTAA AACTCTGGCA 
GATCTCTTGG AATCCTTTAT TGCTGCCCTG TACATTGATA AAGATTTGGA ATATGTTCAC 
ACTTTCATGA ATGTATGCTT TTTTCCACGA CTAAAGGAAT TCATTTTAAA TCAAGATTGG 
AATGACCCTA AATCTCAGCT TCAGCAGTGC TGCTTGACTC TCAGGACAGA AGGAAAGGAG 
CCAGACATTC CTCTTTACAA GACTCTGCAG ACAGTGGGAC CATCTCATGC CAGGACGTAC 
ACTGTAGCTG TTTATTTCAA AGGGGAAAGA ATAGGATGTG GTAAAGGCCC AAGTATTCAA 
CAAGCAGAAA TGGGAGCTGC AATGGATGCA CTTGAAAAAT ATAACTTTCC CCAGATGGCC 
CATCAGAAAC GGTTCATTGA ACGGAAGTAC AGACAAGAGT TGAAAGAAAT GAGGTGGGAA 
AGAGAGCATC AAGAGAGAGA AACAGATGAG ACTGAAGAAG CAAGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( XM_015282182.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137668.1 Gallus gallus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa32168
Clone ID Related Accession (Same CDS sequence)	XM_015282183.1 , XM_015282183.2 , XM_025147270.1 , XM_015282182.1 , XM_015282182.2
Accession Version	XM_015282182.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ribonuclease 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTCC ATTCTGGACG AGGATGTCCC CGTGGGCAGG GGGGACCAGG AGCAAGAACT 
TCAGCACAGA CGTATCGTCC TCAGAACCTG CGGCAGCTTC ACCCCCAGCA GCCTTCTGTA 
CAGTACCAGT ATGACCAGCA AGCCGCACCT GCAGCTACCT ATTCAAACCC TTCAGCCACC 
AGTTACATGC CTCCCAGGCC AGACTTTGTT CCATATCCCC CACCAGTTCC CCCTTCCACA 
CAAAATCCCA TCAACCAGTG TCCCATGAGG CCACCGTTTC CAAACCATCA GATGAGGCAG 
AGCTTCCCAG TGCCTCCATG CTTCCCTCCT GCTCCTCCAC CAGTGCCAGC CCCTAGCAAT 
GCTCCCGTGC CAGCAACTGC TACTGGGCAG AGCACCTTTC CATACATGAT GCCTCCTCCC 
GCAGTACCCC ATCCTCCCCC ACCACCAGTC ATACCACAGC AAGTCAACTA CCAGCCTGTA 
TATTCCACAG GGTATTCCCA GCAGTCATTC CCACCACCTA ACTTCAATAG CTATCAGCAC 
AACTCTGGCT CCTTTCAGAG CAGCACTACC AATAGCAGCA GTGGCAGCAG CCATTTTCGG 
CACACGGGTC AGTACCAGGG AGAAAAGTCA CAAAGTGATC GACGATCTCC GGAGAGGAGC 
AAGCACTACG ACGAGCACCG GCACCGTGAG CACAGTCACA TCCACGGTGA CAGGCATCGA 
TCCAATAACC ACGGGGATCG TCGGGATCGA GGCCGCAGTC CAGACAGGAG GAGGCAGGAA 
AGCAGTCGGC ACCGGACTGA ATATGACAGA GGAAGAATGT CGCCTCATCA TAGAAGTTAT 
GAGCGTAGCA GGGAGCGTGA GAGGGACAGG CATAGGCACC GTGACAGCAG AAGATCACCA 
TCACCGGACA GGTCCTACAA AAAAGATTAT AAAAGAGCAG GAAGCCGCTC TCCAAGTAGA 
GAAAGAAAGA GACCCCGATG GGAGGAGGAA AGAGAAAGGT GGTCTGAGAG CCAGAGTGCC 
AACAAAGAAA AAAACTACAC TAGTATCAAA GACAAAGAGT CAGAGGAGAA TGCATCTGAA 
AAAAATGAAG ATGAAGATGA TGAATTACTT AAACCAGTCT GGGTTCGCTG TACCCATTCT 
GAAAGCTATT ATTCAAATGA CCCCATGGAT CAAGTGGGGG ACTCTACTGT GGTAGGCACA 
AGCAAGCTCC GAGATCTGTA TGAGAAGTTT GAGGAGGAGC TAGGAAAACG ACAGGCAAAG 
GCCAAAGCAG CCAGGCCACC ATGGGAGCCG CCGAAGACCA AGCTGGATGA AGATCTTGAG 
AGCTCCAGTG AGTCAGAATG CGACTCTGAT GATGACAGCA GCTGCTCCAG CAGTTCAGAT 
TCGGAAGTTT TTGATGTCAT TGCAGAAATT AAGCGTAAGA AAGCACACCC TGATCGTCTT 
CATGAGGAAC TGTGGTACAA TGATCCAGGA CAGATGAATG ATGGACCGCT GTGCAAGTGC 
AGCGCTAAAG CCCGACGAAC AGGGATAAGA CATGGCATTT ATCCTGGTGA AGAGCCCATT 
AAACCATGTC GCCCCATGAC CAACAATGCT GGAAGACTGT ACCATTATCG AATTACAGTG 
TCACCTCCCA CAAACTTCTT AACAGACAGA CCTACTGTTA TTGAGTATGA TGACCATGAA 
TATATCTTCG AAGGATTCTC CATGTTTTCA CATGCCCCAC TGACAAACAT TCCTCTATGT 
AAAGTAATCA GATTTAACAT TGACTATACA ATTCATTTCA TTGAAGAGAT GATGCCTGAG 
AATTTTTGTG TGAAAGGTCT TGAGCTGTTC TCTTCTTATC TATTCAAAGA TATTCTGGAG 
CTGTATGACT GGCATCTTAA AGGTCCTTCA GTTGAAAATG ATTCTGTTTC CTGCCCCAGA 
TTTCACTTCA TGCCGCGATT TGTGAGATTT CTTCCAGATG GAGGGAAGGA AGTACTGTCA 
ATGCATCAGA TTCTTCTCTA CTTGTTACGA TGCAATAAAG CTTTGGTGCC TGAAGAAGAG 
ATTGCCAACA TGCTTCAGTG GGAAGAGCTG GAATGGCAGA AATATGCAGA GGAATGCAAA 
GGGATGATCG TTACCAGCCC TGGCATGAAA CCCAGCTCAG TTCGTATTGA TCAACTGGAC 
CGTGAACAAT TCAATCCTGA TGTAATTACT TTCCCCATTA TTGTCCACTT TGGGATACGA 
CCTGCTCAAC TCAGCTACGC TGGAGATCCT CAATACCAAA AACTATGGAA GAGTTATGTG 
AAGCTGCGTC ACCTGCTAGC TAACAGTCCC AAAGTCAAAC AAGCTGATAA ACAGAAATTA 
TCACAGAGAG AGGAAGCACT GCAGAAAATC CGGCAGAAGA ACACGATGAG ACGTGAAGTG 
ACTGTCGAGT TGAGTAGCCA GGGATTCTGG AAGACGGGGA TACGCTCTGA TGTCTGCCAG 
CACGCAATGA TGCTTCCTGT CCTCACCCAC CATATTCGCT ACCATCAGTG TCTGATGCAT 
TTGGACAAGT TGATAGGCTA CACTTTTCGA GATAGGTGCC TGCTGCAGCT TGCCATGACT 
CATCCGAGCC ATCACTTAAA CTTTGGAATG AATCCAGACC ATGCCAGAAA TTCCTTATCC 
AACTGTGGGA TTCGACAGCC AAAGTATGGG GACAGAAAAG TTCACCATAT GCATATGAGA 
AAAAAAGGGA TAAACACCCT GATAAATATT ATGTCCCGTT TGGGACAGGA TGATCCAGCT 
CCTTCCAGGA TTAACCACAA TGAGCGTTTA GAATTTCTGG GAGATGCTGT GGTGGAGTTC 
TTAACAAGTG TCCACTTGTA CTACCTGTTT CCCACTCTGG AGGAAGGAGG ATTAGCTACG 
TACCGCACTG CCATAGTTCA GAACCAGCAT CTGGCCATGC TGGCTAAGAA GCTGGAACTA 
GATCGATTTA TGCTTTATGC TCATGGTCCG GACCTTTGCA GGGAATCGGA TCTCCGCCAT 
GCCATGGCCA ACTGCTTTGA AGCACTAATA GGAGCAGTTT ATCTGGAGGG TAGTCTTGAA 
GAAGCCAAAC AATTATTTGG ACGGTTGCTC TTCAATGACA AGGACCTGCG GGATGTATGG 
CTTAATTATC CACTACACCC ACTCCAACTG CAGGAGTCTA ATACTGACAG GCAACTCATT 
GAGACCTCTC CAGTTCTTCA AAAGCTTACG GAGTTTGAGG AGGCAATTGG AGTCATCTTT 
ACTCATGTTC GACTTCTAGC ACGTGCATTC ACTCTGAGGA CAGTAGGCTT TAACCACCTG 
ACTCTAGGCC ACAATCAGAG GATGGAATTC CTGGGTGACT CCATAATGCA GTTGGTAGCC 
ACAGAGTATT TATTCATACA TTTCCCCGAT CATCATGAAG GGCACTTAAC GCTGTTGAGA 
AGCTCTCTGG TGAACAACAG GACTCAGGCC AAAGTGGCAG AAGAGCTGGG AATGCAAGAG 
TTTGCCATCA CTAATGACAA GACCAAAAGA CCCGTAGCTC TTCGAACTAA AACTCTGGCA 
GATCTCTTGG AATCCTTTAT TGCTGCCCTG TACATTGATA AAGATTTGGA ATATGTTCAC 
ACTTTCATGA ATGTATGCTT TTTTCCACGA CTAAAGGAAT TCATTTTAAA TCAAGATTGG 
AATGACCCTA AATCTCAGCT TCAGCAGTGC TGCTTGACTC TCAGGACAGA AGGAAAGGAG 
CCAGACATTC CTCTTTACAA GACTCTGCAG ACAGTGGGAC CATCTCATGC CAGGACGTAC 
ACTGTAGCTG TTTATTTCAA AGGGGAAAGA ATAGGATGTG GTAAAGGCCC AAGTATTCAA 
CAAGCAGAAA TGGGAGCTGC AATGGATGCA CTTGAAAAAT ATAACTTTCC CCAGATGGCC 
CATCAGAAAC GGTTCATTGA ACGGAAGTAC AGACAAGAGT TGAAAGAAAT GAGGTGGGAA 
AGAGAGCATC AAGAGAGAGA AACAGATGAG ACTGAAGAAG CAAGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137668.1
CDS	283..4293
Translation	MSFHSGRGCPRGQGGPGARTSAQTYRPQNLRQLHPQQPSVQYQYDQQAAPAATYSNPSATSYMPPRPDFVPYPPPVPPSTQNPINQCPMRPPFPNHQMRQSFPVPPCFPPAPPPVPAPSNAPVPATATGQSTFPYMMPPPAVPHPPPPPVIPQQVNYQPVYSTGYSQQSFPPPNFNSYQHNSGSFQSSTTNSSSGSSHFRHTGQYQGEKSQSDRRSPERSKHYDEHRHREHSHIHGDRHRSNNHGDRRDRGRSPDRRRQESSRHRTEYDRGRMSPHHRSYERSRERERDRHRHRDSRRSPSPDRSYKKDYKRAGSRSPSRERKRPRWEEERERWSESQSANKEKNYTSIKDKESEENASEKNEDEDDELLKPVWVRCTHSESYYSNDPMDQVGDSTVVGTSKLRDLYEKFEEELGKRQAKAKAARPPWEPPKTKLDEDLESSSESECDSDDDSSCSSSSDSEVFDVIAEIKRKKAHPDRLHEELWYNDPGQMNDGPLCKCSAKARRTGIRHGIYPGEEPIKPCRPMTNNAGRLYHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFSHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSSYLFKDILELYDWHLKGPSVENDSVSCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCNKALVPEEEIANMLQWEELEWQKYAEECKGMIVTSPGMKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQADKQKLSQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFRDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPAPSRINHNERLEFLGDAVVEFLTSVHLYYLFPTLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDKDLRDVWLNYPLHPLQLQESNTDRQLIETSPVLQKLTEFEEAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEFAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQERETDETEEARK

Target ORF information:

RefSeq Version	XM_015282182.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282182.2

ORF Insert Sequence:

ATGTCTTTCC ATTCTGGACG AGGATGTCCC CGTGGGCAGG GGGGACCAGG AGCAAGAACT 
TCAGCACAGA CGTATCGTCC TCAGAACCTG CGGCAGCTTC ACCCCCAGCA GCCTTCTGTA 
CAGTACCAGT ATGACCAGCA AGCCGCACCT GCAGCTACCT ATTCAAACCC TTCAGCCACC 
AGTTACATGC CTCCCAGGCC AGACTTTGTT CCATATCCCC CACCAGTTCC CCCTTCCACA 
CAAAATCCCA TCAACCAGTG TCCCATGAGG CCACCGTTTC CAAACCATCA GATGAGGCAG 
AGCTTCCCAG TGCCTCCATG CTTCCCTCCT GCTCCTCCAC CAGTGCCAGC CCCTAGCAAT 
GCTCCCGTGC CAGCAACTGC TACTGGGCAG AGCACCTTTC CATACATGAT GCCTCCTCCC 
GCAGTACCCC ATCCTCCCCC ACCACCAGTC ATACCACAGC AAGTCAACTA CCAGCCTGTA 
TATTCCACAG GGTATTCCCA GCAGTCATTC CCACCACCTA ACTTCAATAG CTATCAGCAC 
AACTCTGGCT CCTTTCAGAG CAGCACTACC AATAGCAGCA GTGGCAGCAG CCATTTTCGG 
CACACGGGTC AGTACCAGGG AGAAAAGTCA CAAAGTGATC GACGATCTCC GGAGAGGAGC 
AAGCACTACG ACGAGCACCG GCACCGTGAG CACAGTCACA TCCACGGTGA CAGGCATCGA 
TCCAATAACC ACGGGGATCG TCGGGATCGA GGCCGCAGTC CAGACAGGAG GAGGCAGGAA 
AGCAGTCGGC ACCGGACTGA ATATGACAGA GGAAGAATGT CGCCTCATCA TAGAAGTTAT 
GAGCGTAGCA GGGAGCGTGA GAGGGACAGG CATAGGCACC GTGACAGCAG AAGATCACCA 
TCACCGGACA GGTCCTACAA AAAAGATTAT AAAAGAGCAG GAAGCCGCTC TCCAAGTAGA 
GAAAGAAAGA GACCCCGATG GGAGGAGGAA AGAGAAAGGT GGTCTGAGAG CCAGAGTGCC 
AACAAAGAAA AAAACTACAC TAGTATCAAA GACAAAGAGT CAGAGGAGAA TGCATCTGAA 
AAAAATGAAG ATGAAGATGA TGAATTACTT AAACCAGTCT GGGTTCGCTG TACCCATTCT 
GAAAGCTATT ATTCAAATGA CCCCATGGAT CAAGTGGGGG ACTCTACTGT GGTAGGCACA 
AGCAAGCTCC GAGATCTGTA TGAGAAGTTT GAGGAGGAGC TAGGAAAACG ACAGGCAAAG 
GCCAAAGCAG CCAGGCCACC ATGGGAGCCG CCGAAGACCA AGCTGGATGA AGATCTTGAG 
AGCTCCAGTG AGTCAGAATG CGACTCTGAT GATGACAGCA GCTGCTCCAG CAGTTCAGAT 
TCGGAAGTTT TTGATGTCAT TGCAGAAATT AAGCGTAAGA AAGCACACCC TGATCGTCTT 
CATGAGGAAC TGTGGTACAA TGATCCAGGA CAGATGAATG ATGGACCGCT GTGCAAGTGC 
AGCGCTAAAG CCCGACGAAC AGGGATAAGA CATGGCATTT ATCCTGGTGA AGAGCCCATT 
AAACCATGTC GCCCCATGAC CAACAATGCT GGAAGACTGT ACCATTATCG AATTACAGTG 
TCACCTCCCA CAAACTTCTT AACAGACAGA CCTACTGTTA TTGAGTATGA TGACCATGAA 
TATATCTTCG AAGGATTCTC CATGTTTTCA CATGCCCCAC TGACAAACAT TCCTCTATGT 
AAAGTAATCA GATTTAACAT TGACTATACA ATTCATTTCA TTGAAGAGAT GATGCCTGAG 
AATTTTTGTG TGAAAGGTCT TGAGCTGTTC TCTTCTTATC TATTCAAAGA TATTCTGGAG 
CTGTATGACT GGCATCTTAA AGGTCCTTCA GTTGAAAATG ATTCTGTTTC CTGCCCCAGA 
TTTCACTTCA TGCCGCGATT TGTGAGATTT CTTCCAGATG GAGGGAAGGA AGTACTGTCA 
ATGCATCAGA TTCTTCTCTA CTTGTTACGA TGCAATAAAG CTTTGGTGCC TGAAGAAGAG 
ATTGCCAACA TGCTTCAGTG GGAAGAGCTG GAATGGCAGA AATATGCAGA GGAATGCAAA 
GGGATGATCG TTACCAGCCC TGGCATGAAA CCCAGCTCAG TTCGTATTGA TCAACTGGAC 
CGTGAACAAT TCAATCCTGA TGTAATTACT TTCCCCATTA TTGTCCACTT TGGGATACGA 
CCTGCTCAAC TCAGCTACGC TGGAGATCCT CAATACCAAA AACTATGGAA GAGTTATGTG 
AAGCTGCGTC ACCTGCTAGC TAACAGTCCC AAAGTCAAAC AAGCTGATAA ACAGAAATTA 
TCACAGAGAG AGGAAGCACT GCAGAAAATC CGGCAGAAGA ACACGATGAG ACGTGAAGTG 
ACTGTCGAGT TGAGTAGCCA GGGATTCTGG AAGACGGGGA TACGCTCTGA TGTCTGCCAG 
CACGCAATGA TGCTTCCTGT CCTCACCCAC CATATTCGCT ACCATCAGTG TCTGATGCAT 
TTGGACAAGT TGATAGGCTA CACTTTTCGA GATAGGTGCC TGCTGCAGCT TGCCATGACT 
CATCCGAGCC ATCACTTAAA CTTTGGAATG AATCCAGACC ATGCCAGAAA TTCCTTATCC 
AACTGTGGGA TTCGACAGCC AAAGTATGGG GACAGAAAAG TTCACCATAT GCATATGAGA 
AAAAAAGGGA TAAACACCCT GATAAATATT ATGTCCCGTT TGGGACAGGA TGATCCAGCT 
CCTTCCAGGA TTAACCACAA TGAGCGTTTA GAATTTCTGG GAGATGCTGT GGTGGAGTTC 
TTAACAAGTG TCCACTTGTA CTACCTGTTT CCCACTCTGG AGGAAGGAGG ATTAGCTACG 
TACCGCACTG CCATAGTTCA GAACCAGCAT CTGGCCATGC TGGCTAAGAA GCTGGAACTA 
GATCGATTTA TGCTTTATGC TCATGGTCCG GACCTTTGCA GGGAATCGGA TCTCCGCCAT 
GCCATGGCCA ACTGCTTTGA AGCACTAATA GGAGCAGTTT ATCTGGAGGG TAGTCTTGAA 
GAAGCCAAAC AATTATTTGG ACGGTTGCTC TTCAATGACA AGGACCTGCG GGATGTATGG 
CTTAATTATC CACTACACCC ACTCCAACTG CAGGAGTCTA ATACTGACAG GCAACTCATT 
GAGACCTCTC CAGTTCTTCA AAAGCTTACG GAGTTTGAGG AGGCAATTGG AGTCATCTTT 
ACTCATGTTC GACTTCTAGC ACGTGCATTC ACTCTGAGGA CAGTAGGCTT TAACCACCTG 
ACTCTAGGCC ACAATCAGAG GATGGAATTC CTGGGTGACT CCATAATGCA GTTGGTAGCC 
ACAGAGTATT TATTCATACA TTTCCCCGAT CATCATGAAG GGCACTTAAC GCTGTTGAGA 
AGCTCTCTGG TGAACAACAG GACTCAGGCC AAAGTGGCAG AAGAGCTGGG AATGCAAGAG 
TTTGCCATCA CTAATGACAA GACCAAAAGA CCCGTAGCTC TTCGAACTAA AACTCTGGCA 
GATCTCTTGG AATCCTTTAT TGCTGCCCTG TACATTGATA AAGATTTGGA ATATGTTCAC 
ACTTTCATGA ATGTATGCTT TTTTCCACGA CTAAAGGAAT TCATTTTAAA TCAAGATTGG 
AATGACCCTA AATCTCAGCT TCAGCAGTGC TGCTTGACTC TCAGGACAGA AGGAAAGGAG 
CCAGACATTC CTCTTTACAA GACTCTGCAG ACAGTGGGAC CATCTCATGC CAGGACGTAC 
ACTGTAGCTG TTTATTTCAA AGGGGAAAGA ATAGGATGTG GTAAAGGCCC AAGTATTCAA 
CAAGCAGAAA TGGGAGCTGC AATGGATGCA CTTGAAAAAT ATAACTTTCC CCAGATGGCC 
CATCAGAAAC GGTTCATTGA ACGGAAGTAC AGACAAGAGT TGAAAGAAAT GAGGTGGGAA 
AGAGAGCATC AAGAGAGAGA AACAGATGAG ACTGAAGAAG CAAGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DROSHA ( NM_001006379.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001006379.1 Gallus gallus drosha ribonuclease III (DROSHA), mRNA.

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CloneID	OGa25819
Clone ID Related Accession (Same CDS sequence)	NM_001006379.1
Accession Version	NM_001006379.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	ribonuclease 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ720721.1. On Jan 13, 2005 this sequence version replaced XM_418997.1. ##Evidence-Data-START## Transcript exon combination :: AJ720721.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCTTTCC ATTCTGGACG AGGATGTCCC CGTGGGCAGG GGGGACCAGG AGCAAGAACT 
TCAGCACAGA CGTATCGTCC TCAGAACCTG CGGCAGCTTC ACCCCCAGCA GCCTTCTGTA 
CAGTACCAGT ATGACCAGCA AGCCGCACCT GCAGCTACCT ATTCAAACCC TTCAGCCACC 
AGTTACATGC CTCCCAGGCC AGACTTTGTT CCATATCCCC CACCAGTTCC CCCTTCCACA 
CAAAATCCCA TCAACCAGTG TCCCATGAGG CCACCGTTTC CAAACCATCA GATGAGGCAG 
AGCTTCCCAG TGCCTCCATG CTTCCCTCCT GCTCCTCCAC CAGTGCCAGC CCCTAGCAAT 
GCTCCCGTGC CAGCAACTGC TACTGGGCAG AGTACCTTTC CATACATGAT GCCTCCTCCC 
GCAGTACCCC ATCCTCCCCC ACCACCAGTC ATACCACAGC AAGTCAACTA CCAGCCTGTA 
TATTCCACAG GGTATTCCCA GCAGTCATTC CCACCACCTA ACTTCAATAG CTATCAGCAC 
AACTCTAGCT CCTTTCAGAG CAGCACTACC AATAGCAGCA GTGGCAGCAG CCATTTTCGG 
CACACGGGTC AGTACCAGGG AGAAAAGTCA CAAAGTGATC GACGATCTCC GGAGAGGAGC 
AAACACTACG ACGAGCACCG GCACCGTGAG CACAGTCACA TCCACGGTGA CAGGCATCGA 
TCCAATAACC ACGGGGATCG TCGGGATCGA GGCCGCAGTC CAGACAGGAG GAGGCAGGAA 
AGCAGTCGGC ACCGGACTGA ATATGACAGA GGAAGAATGT CGCCTCATCA TAGAAGTTAT 
GAGCGTAGCA GGGAGCGTGA CAGGGATAGG CATAGGCACC GTGACAGCAG AAGATCACCA 
TCACCGGACA GGTCCTACAA AAAAGATTAT AAAAGAGCAG GAAGCCGCTC TCCAAGTAGA 
GAAAGAAAGA GACCCCGATG GGAGGAGGAA AGAGAAAGGT GGTCTGAGAG CCAGAGTGCC 
AACAAAGAAA AAAACTACAC TAGTATCAAA GACAAAGAGT CAGAGGAGAA TGCATCTGAA 
AAAAATGAAG ATGAAGATGA TGAATTACTT AAACCAGTCT GGGTTCGCTG TACCCATTCT 
GAAAGCTATT ATTCAAATGA CCCCATGGAT CAAGTGGGGG ACTCTACTGT GGTAGGCACA 
AGCAAGCTCC GAGATCTGTA TGAGAAGTTT GAGGAGGAGC TAGGAAAACG ACAGGCAAAG 
GCCAAAGCAG CCAGGCCACC ATGGGAGCCG CCGAAGACCA AGCTGGATGA AGATCTTGAG 
AGCTCCAGTG AGTCAGAATG CGACTCTGAT GATGACAGCA GCTGCTCCAG CAGTTCAGAT 
TCGGAAGTTT TTGATGTCAT TGCAGAAATT AAGCGTAAGA AAGCACACCC TGATCGTCTT 
CATGAGGAAC TGTGGTACAA TGATCCAGGA CAGATGAATG ATGGACCGCT GTGCAAGTGC 
AGCGCTAAAG CCCGACGAAC AGGGATAAGA CATGGCATTT ATCCTGGTGA AGAGCCCATT 
AAACCATGTC GCCCCATGAC CAACAATGCT GGAAGACTGT ACCATTATCG AATTACAGTG 
TCACCTCCCA CAAACTTCTT AACAGACAGA CCTACTGTTA TTGAGTATGA TGACCATGAA 
TATATCTTCG AAGGATTCTC CATGTTTTCA CATGCCCCAC TGACAAACAT TCCTCTATGT 
AAAGTAATCA GATTTAACAT TGACTATACA ATTCATTTCA TTGAAGAGAT GATGCCTGAG 
AATTTTTGTG TGAAAGGTCT TGAGCTGTTC TCTTCTTATC TATTCAAAGA TATTCTGGAG 
CTGTATGACT GGCATCTTAA AGGTCCTTCA GTTGAAAATG ATTCTGTTTC CTGCCCCAGA 
TTTCACTTCA TGCCGCGATT TGTGAGATTT CTTCCAGATG GAGGGAAGGA AGTACTGTCA 
ATGCATCAGA TTCTTCTCTA CTTGTTACGA TGCAATAAAG CTTTGGTGCC TGAAGAAGAG 
ATTGCCAACA TGCTTCAGTG GGAAGAGCTG GAATGGCAGA AATATGCAGA GGAATGCAAA 
GGGATGATCG TTACCAGCCC TGGCATGAAA CCCAGCTCAG TTCGTATTGA TCAACTGGAC 
CGTGAACAAT TCAATCCTGA TGTAATTACT TTCCCCATTA TTGTCCACTT TGGGATACGA 
CCTGCTCAAC TCAGCTACGC TGGAGATCCT CAATACCAAA AACTATGGAA GAGTTATGTG 
AAGCTGCGTC ACCTGCTAGC TAACAGTCCC AAAGTCAAAC AAGCTGATAA ACAGAAATTA 
TCACAGAGAG AGGAAGCACT GCAGAAAATC CGGCAGAAGA ACACGATGAG ACGTGAAGTG 
ACTGTCGAGT TGAGTAGCCA GGGATTCTGG AAGACGGGGA TACGCTCTGA TGTCTGCCAG 
CACGCAATGA TGCTTCCTGT CCTCACCCAC CATATTCGCT ACCATCAGTG TCTGATGCAT 
TTGGACAAGT TGATAGGCTA CACTTTTCGA GATAGGTGCC TGCTGCAGCT TGCCATGACT 
CATCCGAGCC ATCACTTAAA CTTTGGAATG AATCCAGACC ATGCCAGAAA TTCCTTATCC 
AACTGTGGGA TTCGACAGCC AAAGTATGGG GACAGAAAAG TTCACCATAT GCATATGAGA 
AAAAAAGGGA TAAACACCCT GATAAATATT ATGTCCCGTT TGGGACAGGA TGATCCAGCT 
CCTTCCAGGA TTAACCACAA TGAGCGTTTA GAATTTCTGG GAGATGCTGT GGTGGAGTTC 
TTAACAAGTG TCCACTTGTA CTACCTGTTT CCCACTCTGG AGGAAGGAGG ATTAGCTACG 
TACCGCACTG CCATAGTTCA GAACCAGCAT CTGGCCATGC TGGCTAAGAA GCTGGAACTA 
GATCGATTTA TGCTTTATGC TCATGGTCCG GACCTTTGCA GGGAATCGGA TCTCCGCCAT 
GCCATGGCCA ACTGCTTTGA AGCACTAATA GGAGCAGTTT ATCTGGAGGG TAGTCTTGAA 
GAAGCCAAAC AATTATTTGG ACGGTTGCTC TTCAATGACA AGGACCTGCG GGATGTATGG 
CTTAATTATC CACTACACCC ACTCCAACTG CAGGAGTCTA ATACTGACAG GCAACTCATT 
GAGACCTCTC CAGTTCTTCA AAAGCTTACG GAGTTTGAGG AGGCAATTGG AGTCATCTTT 
ACTCATGTTC GACTTCTAGC ACGTGCATTC ACTCTGAGGA CAGTAGGCTT TAACCACCTG 
ACTCTAGGCC ACAATCAGAG GATGGAATTC CTGGGTGACT CCATAATGCA GTTGGTAGCC 
ACAGAGTATT TATTCATACA TTTCCCCGAT CATCATGAAG GGCACTTAAC GCTGTTGAGA 
AGCTCTCTGG TGAACAACAG GACTCAGGCC AAAGTGGCAG AAGAGCTGGG AATGCAAGAG 
TTTGCCATCA CTAATGACAA GACCAAAAGA CCCGTAGCTC TTCGAACTAA AACTCTGGCA 
GATCTCTTGG AATCCTTTAT TGCTGCCCTG TACATTGATA AAGATTTGGA ATATGTTCAC 
ACTTTCATGA ATGTATGCTT TTTTCCACGA CTAAAGGAAT TCATTTTAAA TCAAGATTGG 
AATGACCCTA AATCTCAGCT TCAGCAGTGC TGCTTGACTC TCAGGACAGA AGGAAAGGAG 
CCAGACATTC CTCTTTACAA GACTCTGCAG ACAGTGGGAC CATCTCATGC CAGGACGTAC 
ACTGTAGCTG TTTATTTCAA AGGGGAAAGA ATAGGATGTG GTAAAGGCCC AAGTATTCAA 
CAAGCAGAAA TGGGAGCTGC AATGGATGCA CTTGAAAAAT ATAACTTTCC CCAGATGGCC 
CATCAGAAAC GGTTCATTGA ACGGAAGTAC AGACAAGAGT TGAAAGAAAT GAGGTGGGAA 
AGAGAGCATC AAGAGAGAGA AACAGATGAG ACTGAAGAAG CAAGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006379.1
CDS	173..4183
Translation	MSFHSGRGCPRGQGGPGARTSAQTYRPQNLRQLHPQQPSVQYQYDQQAAPAATYSNPSATSYMPPRPDFVPYPPPVPPSTQNPINQCPMRPPFPNHQMRQSFPVPPCFPPAPPPVPAPSNAPVPATATGQSTFPYMMPPPAVPHPPPPPVIPQQVNYQPVYSTGYSQQSFPPPNFNSYQHNSSSFQSSTTNSSSGSSHFRHTGQYQGEKSQSDRRSPERSKHYDEHRHREHSHIHGDRHRSNNHGDRRDRGRSPDRRRQESSRHRTEYDRGRMSPHHRSYERSRERDRDRHRHRDSRRSPSPDRSYKKDYKRAGSRSPSRERKRPRWEEERERWSESQSANKEKNYTSIKDKESEENASEKNEDEDDELLKPVWVRCTHSESYYSNDPMDQVGDSTVVGTSKLRDLYEKFEEELGKRQAKAKAARPPWEPPKTKLDEDLESSSESECDSDDDSSCSSSSDSEVFDVIAEIKRKKAHPDRLHEELWYNDPGQMNDGPLCKCSAKARRTGIRHGIYPGEEPIKPCRPMTNNAGRLYHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFSHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSSYLFKDILELYDWHLKGPSVENDSVSCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCNKALVPEEEIANMLQWEELEWQKYAEECKGMIVTSPGMKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQADKQKLSQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFRDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPAPSRINHNERLEFLGDAVVEFLTSVHLYYLFPTLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDKDLRDVWLNYPLHPLQLQESNTDRQLIETSPVLQKLTEFEEAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEFAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQERETDETEEARK

Target ORF information:

RefSeq Version	NM_001006379.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus drosha ribonuclease III (DROSHA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006379.1

ORF Insert Sequence:

ATGTCTTTCC ATTCTGGACG AGGATGTCCC CGTGGGCAGG GGGGACCAGG AGCAAGAACT 
TCAGCACAGA CGTATCGTCC TCAGAACCTG CGGCAGCTTC ACCCCCAGCA GCCTTCTGTA 
CAGTACCAGT ATGACCAGCA AGCCGCACCT GCAGCTACCT ATTCAAACCC TTCAGCCACC 
AGTTACATGC CTCCCAGGCC AGACTTTGTT CCATATCCCC CACCAGTTCC CCCTTCCACA 
CAAAATCCCA TCAACCAGTG TCCCATGAGG CCACCGTTTC CAAACCATCA GATGAGGCAG 
AGCTTCCCAG TGCCTCCATG CTTCCCTCCT GCTCCTCCAC CAGTGCCAGC CCCTAGCAAT 
GCTCCCGTGC CAGCAACTGC TACTGGGCAG AGTACCTTTC CATACATGAT GCCTCCTCCC 
GCAGTACCCC ATCCTCCCCC ACCACCAGTC ATACCACAGC AAGTCAACTA CCAGCCTGTA 
TATTCCACAG GGTATTCCCA GCAGTCATTC CCACCACCTA ACTTCAATAG CTATCAGCAC 
AACTCTAGCT CCTTTCAGAG CAGCACTACC AATAGCAGCA GTGGCAGCAG CCATTTTCGG 
CACACGGGTC AGTACCAGGG AGAAAAGTCA CAAAGTGATC GACGATCTCC GGAGAGGAGC 
AAACACTACG ACGAGCACCG GCACCGTGAG CACAGTCACA TCCACGGTGA CAGGCATCGA 
TCCAATAACC ACGGGGATCG TCGGGATCGA GGCCGCAGTC CAGACAGGAG GAGGCAGGAA 
AGCAGTCGGC ACCGGACTGA ATATGACAGA GGAAGAATGT CGCCTCATCA TAGAAGTTAT 
GAGCGTAGCA GGGAGCGTGA CAGGGATAGG CATAGGCACC GTGACAGCAG AAGATCACCA 
TCACCGGACA GGTCCTACAA AAAAGATTAT AAAAGAGCAG GAAGCCGCTC TCCAAGTAGA 
GAAAGAAAGA GACCCCGATG GGAGGAGGAA AGAGAAAGGT GGTCTGAGAG CCAGAGTGCC 
AACAAAGAAA AAAACTACAC TAGTATCAAA GACAAAGAGT CAGAGGAGAA TGCATCTGAA 
AAAAATGAAG ATGAAGATGA TGAATTACTT AAACCAGTCT GGGTTCGCTG TACCCATTCT 
GAAAGCTATT ATTCAAATGA CCCCATGGAT CAAGTGGGGG ACTCTACTGT GGTAGGCACA 
AGCAAGCTCC GAGATCTGTA TGAGAAGTTT GAGGAGGAGC TAGGAAAACG ACAGGCAAAG 
GCCAAAGCAG CCAGGCCACC ATGGGAGCCG CCGAAGACCA AGCTGGATGA AGATCTTGAG 
AGCTCCAGTG AGTCAGAATG CGACTCTGAT GATGACAGCA GCTGCTCCAG CAGTTCAGAT 
TCGGAAGTTT TTGATGTCAT TGCAGAAATT AAGCGTAAGA AAGCACACCC TGATCGTCTT 
CATGAGGAAC TGTGGTACAA TGATCCAGGA CAGATGAATG ATGGACCGCT GTGCAAGTGC 
AGCGCTAAAG CCCGACGAAC AGGGATAAGA CATGGCATTT ATCCTGGTGA AGAGCCCATT 
AAACCATGTC GCCCCATGAC CAACAATGCT GGAAGACTGT ACCATTATCG AATTACAGTG 
TCACCTCCCA CAAACTTCTT AACAGACAGA CCTACTGTTA TTGAGTATGA TGACCATGAA 
TATATCTTCG AAGGATTCTC CATGTTTTCA CATGCCCCAC TGACAAACAT TCCTCTATGT 
AAAGTAATCA GATTTAACAT TGACTATACA ATTCATTTCA TTGAAGAGAT GATGCCTGAG 
AATTTTTGTG TGAAAGGTCT TGAGCTGTTC TCTTCTTATC TATTCAAAGA TATTCTGGAG 
CTGTATGACT GGCATCTTAA AGGTCCTTCA GTTGAAAATG ATTCTGTTTC CTGCCCCAGA 
TTTCACTTCA TGCCGCGATT TGTGAGATTT CTTCCAGATG GAGGGAAGGA AGTACTGTCA 
ATGCATCAGA TTCTTCTCTA CTTGTTACGA TGCAATAAAG CTTTGGTGCC TGAAGAAGAG 
ATTGCCAACA TGCTTCAGTG GGAAGAGCTG GAATGGCAGA AATATGCAGA GGAATGCAAA 
GGGATGATCG TTACCAGCCC TGGCATGAAA CCCAGCTCAG TTCGTATTGA TCAACTGGAC 
CGTGAACAAT TCAATCCTGA TGTAATTACT TTCCCCATTA TTGTCCACTT TGGGATACGA 
CCTGCTCAAC TCAGCTACGC TGGAGATCCT CAATACCAAA AACTATGGAA GAGTTATGTG 
AAGCTGCGTC ACCTGCTAGC TAACAGTCCC AAAGTCAAAC AAGCTGATAA ACAGAAATTA 
TCACAGAGAG AGGAAGCACT GCAGAAAATC CGGCAGAAGA ACACGATGAG ACGTGAAGTG 
ACTGTCGAGT TGAGTAGCCA GGGATTCTGG AAGACGGGGA TACGCTCTGA TGTCTGCCAG 
CACGCAATGA TGCTTCCTGT CCTCACCCAC CATATTCGCT ACCATCAGTG TCTGATGCAT 
TTGGACAAGT TGATAGGCTA CACTTTTCGA GATAGGTGCC TGCTGCAGCT TGCCATGACT 
CATCCGAGCC ATCACTTAAA CTTTGGAATG AATCCAGACC ATGCCAGAAA TTCCTTATCC 
AACTGTGGGA TTCGACAGCC AAAGTATGGG GACAGAAAAG TTCACCATAT GCATATGAGA 
AAAAAAGGGA TAAACACCCT GATAAATATT ATGTCCCGTT TGGGACAGGA TGATCCAGCT 
CCTTCCAGGA TTAACCACAA TGAGCGTTTA GAATTTCTGG GAGATGCTGT GGTGGAGTTC 
TTAACAAGTG TCCACTTGTA CTACCTGTTT CCCACTCTGG AGGAAGGAGG ATTAGCTACG 
TACCGCACTG CCATAGTTCA GAACCAGCAT CTGGCCATGC TGGCTAAGAA GCTGGAACTA 
GATCGATTTA TGCTTTATGC TCATGGTCCG GACCTTTGCA GGGAATCGGA TCTCCGCCAT 
GCCATGGCCA ACTGCTTTGA AGCACTAATA GGAGCAGTTT ATCTGGAGGG TAGTCTTGAA 
GAAGCCAAAC AATTATTTGG ACGGTTGCTC TTCAATGACA AGGACCTGCG GGATGTATGG 
CTTAATTATC CACTACACCC ACTCCAACTG CAGGAGTCTA ATACTGACAG GCAACTCATT 
GAGACCTCTC CAGTTCTTCA AAAGCTTACG GAGTTTGAGG AGGCAATTGG AGTCATCTTT 
ACTCATGTTC GACTTCTAGC ACGTGCATTC ACTCTGAGGA CAGTAGGCTT TAACCACCTG 
ACTCTAGGCC ACAATCAGAG GATGGAATTC CTGGGTGACT CCATAATGCA GTTGGTAGCC 
ACAGAGTATT TATTCATACA TTTCCCCGAT CATCATGAAG GGCACTTAAC GCTGTTGAGA 
AGCTCTCTGG TGAACAACAG GACTCAGGCC AAAGTGGCAG AAGAGCTGGG AATGCAAGAG 
TTTGCCATCA CTAATGACAA GACCAAAAGA CCCGTAGCTC TTCGAACTAA AACTCTGGCA 
GATCTCTTGG AATCCTTTAT TGCTGCCCTG TACATTGATA AAGATTTGGA ATATGTTCAC 
ACTTTCATGA ATGTATGCTT TTTTCCACGA CTAAAGGAAT TCATTTTAAA TCAAGATTGG 
AATGACCCTA AATCTCAGCT TCAGCAGTGC TGCTTGACTC TCAGGACAGA AGGAAAGGAG 
CCAGACATTC CTCTTTACAA GACTCTGCAG ACAGTGGGAC CATCTCATGC CAGGACGTAC 
ACTGTAGCTG TTTATTTCAA AGGGGAAAGA ATAGGATGTG GTAAAGGCCC AAGTATTCAA 
CAAGCAGAAA TGGGAGCTGC AATGGATGCA CTTGAAAAAT ATAACTTTCC CCAGATGGCC 
CATCAGAAAC GGTTCATTGA ACGGAAGTAC AGACAAGAGT TGAAAGAAAT GAGGTGGGAA 
AGAGAGCATC AAGAGAGAGA AACAGATGAG ACTGAAGAAG CAAGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Genome biology6(1)R6(2005)
Caldwell RB,Kierzek AM,Arakawa H,Bezzubov Y,Zaim J,Fiedler P,Kutter S,Blagodatski A,Kostovska D,Koter M,Plachy J,Carninci P,Hayashizaki Y,Buerstedde JM

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オンライン注文

DROSHA cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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