TNIK gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010801393.1	XP_010799695.1	TRAF2 and NCK-interacting protein kinase isoform X4
XM_003585675.3	XP_003585723.1	TRAF2 and NCK-interacting protein kinase isoform X9
XM_003585674.4	XP_003585722.1	TRAF2 and NCK-interacting protein kinase isoform X2
XM_003585673.4	XP_003585721.1	TRAF2 and NCK-interacting protein kinase isoform X3
XM_005195922.2	XP_005195979.1	TRAF2 and NCK-interacting protein kinase isoform X1
XM_010824381.1	XP_010822683.1	TRAF2 and NCK-interacting protein kinase isoform X2
XM_010801394.1	XP_010799696.1	TRAF2 and NCK-interacting protein kinase isoform X5
XM_003585674.3	XP_003585722.1	TRAF2 and NCK-interacting protein kinase isoform X6
XM_005201707.2	XP_005201764.1	TRAF2 and NCK-interacting protein kinase isoform X1
XM_002684922.4	XP_002684968.1	TRAF2 and NCK-interacting protein kinase isoform X3
XM_010801395.1	XP_010799697.1	TRAF2 and NCK-interacting protein kinase isoform X7
XM_588438.8	XP_588438.5	TRAF2 and NCK-interacting protein kinase isoform X3
XM_024995475.1	XP_024851243.1	TRAF2 and NCK-interacting protein kinase isoform X4
XM_024995482.1	XP_024851250.1	TRAF2 and NCK-interacting protein kinase isoform X6
XM_010824382.1	XP_010822684.1	TRAF2 and NCK-interacting protein kinase isoform X4
XM_003581731.3	XP_003581779.1	TRAF2 and NCK-interacting protein kinase isoform X6
XM_010824384.1	XP_010822686.1	TRAF2 and NCK-interacting protein kinase isoform X7
XM_010801392.1	XP_010799694.1	TRAF2 and NCK-interacting protein kinase isoform X2
XM_003585673.3	XP_003585721.1	TRAF2 and NCK-interacting protein kinase isoform X8
XM_002684922.5	XP_002684968.1	TRAF2 and NCK-interacting protein kinase isoform X1
XM_003585675.4	XP_003585723.1	TRAF2 and NCK-interacting protein kinase isoform X5
XM_010824383.1	XP_010822685.1	TRAF2 and NCK-interacting protein kinase isoform X5
XM_003581730.3	XP_003581778.1	TRAF2 and NCK-interacting protein kinase isoform X8
XM_003581732.3	XP_003581780.1	TRAF2 and NCK-interacting protein kinase isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa219204	XM_024995475.1 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa219205	XM_024995482.1 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa02614	XM_003581730.3 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa02614	XM_003585673.4 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa02615	XM_003581732.3 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa02615	XM_003585675.4 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa02611	XM_005201707.2 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa02611	XM_005195922.2 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa02612	XM_002684922.5 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa02612	XM_588438.8 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa02613	XM_003581731.3 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa02613	XM_003585674.4 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa54384	XM_010824381.1 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa54384	XM_010801392.1 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa54385	XM_010824382.1 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa54385	XM_010801393.1 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa54386	XM_010801394.1 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa54386	XM_010824383.1 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa54387	XM_010801395.1 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa54387	XM_010824384.1 Latest version!	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OBa02613	XM_003585674.3	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa02614	XM_003585673.3	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa02612	XM_002684922.4	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa02615	XM_003585675.3	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

TNIK ( XM_024995475.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851243.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa219204
Clone ID Related Accession (Same CDS sequence)	XM_024995475.1
Accession Version	XM_024995475.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGGAGGCTC TGAATGTGAG CTCTCACCGC 
GTCGAGATGC CACGCCAGAA CTCAGATCCC ACCTCTGAAA ACCCACCTCT CCCCACTCGC 
ATTGAGAAGT TTGACCGAAG CTCTTGGTTA CGACAGGAAG AAGACATTCC ACCAAAGGTG 
CCTCAAAGAA CAACTTCTAT ATCCCCAGCA TTAGCAAGGA AGAATTCTCC TGGAAATGGT 
AGTGCTCTGG GACCCAGACT AGGATCTCAA CCCATCAGAG CAAGCAACCC CGACCTGCGG 
AGAACCGAGC CCGTCTTGGA GAGCCCCTTG CAGAGGACGA GCAGTGGCAG TTCTTCCAGC 
TCCAGCACCC CCAGCTCCCA GCCCAGCTCC CAAGGAGGCT CTCAGCCTGG GTCACAAGCA 
GGATCCAGCG AGCGGACCCG GGTTCGAGCC AACAGTAAGT CGGAAGGATC ACCTGTGCTC 
CCCCATGAGC CCTCCAAGGT GAAACCAGAA GAATCCAGGG AAATTACCCG ACCAAGTCGC 
CCAGCTAGCT ATAAGAAAGC TATAGATGAG GATCTGACGG CATTGGCCAA AGAATTAAGA 
GAACTTCGGA TTGAAGAAAC AAACCGCCCA CTGAAGAAGG TAACAGATTA CTCCTCCTCC 
AGTGAGGAGT CAGAAAGCAG TGATGAAGAG GAGGAAGATG GAGAGAGTGA GACCCAGGAT 
GGGACGGTGG CTGTCAGCGA CATACCCAGA CTGATACCCA CAGGAGCCCC CGGGAGCGCT 
GAACAGTACA ATGTGGGAAT GGTCGGCACA CACGCGCTGG AGACCTCTCA TGCAGATACT 
TTCGGCAGCA GTATTTCAAG AGAAGGAACC TTGATGATAA GAGAACCGTC GGGAGAGAAG 
AAGCGACCTG GCCACAGTGA CAGCAATGGC TTTGCCGGCC ACATCAACCT CCCGGACCTG 
GTACAGCAGA GCCATTCCCC TGCGGGGACC CCCACCGAGG GCCTGGGGCG CGTCTCCACC 
CACCCCCAGG AAATGGACGC CGGGACTGAA TATGGCCTGG GGAGCAGCAC CAAAGCCTCC 
TTCACCCCCT TTGTGGACCC CAGGGTATAC CAGACATCTC CCACTGATGA AGATGAAGAG 
GATGAGGAAT CATCAGCTGC GGCTCTGTTT ACTAGCGAAC TTCTTAGGCA AGAACAGGCC 
AAACTAAATG AAGCAAGAAA GATTTCAGTG GTAAATGTAA ACCCAACCAA CATTCGGCCT 
CATAGCGACA CACCGGAGAT CAGAAAATAT AAGAAGCGAT TCAATTCAGA AATACTTTGT 
GCAGCTCTGT GGGGTGTAAA CCTCCTGGTG GGAACCGAAA ATGGCCTGAT GCTTTTGGAC 
CGAAGTGGGC AAGGCAAAGT TTATAATCTG ATCAACCGGA GGCGATTTCA GCAGATGGAT 
GTGCTCGAGG GACTGAATGT CCTCGTGACA ATATCAGGAA AGAAGAATAA GTTACGAGTT 
TACTATCTTT CATGGTTAAG AAACAGAATA CTACACAATG ACCCAGAAGT AGAAAAGAAA 
CAAGGCTGGA TCACTGTGGG AGACTTGGAA GGCTGTATAC ATTACAAAGT TGTTAAATAT 
GAAAGGATCA AATTCTTGGT GATTGCCTTA AAGAATGCTG TGGAGATATA TGCTTGGGCT 
CCTAAACCGT ATCATAAGTT CATGGCATTT AAGTCTTTTG CAGATCTCCA GCACAAGCCA 
CTGCTTGTTG ATCTCACAGT AGAAGAAGGT CAAAGATTAA AGGTTATTTT TGGTTCACAC 
ACTGGTTTCC ATGTAATTGA TGTTGATTCA GGAAACTCTT ATGATATCTA CATACCATCT 
CATATTCAGG GCAATATCAC TCCTCATGCC ATTGTCATCT TGCCTAAGAC AGATGGAATG 
GAAATGCTTG TTTGCTATGA GGATGAGGGG GTGTATGTAA ACACCTACGG CCGGATAACT 
AAGGATGTGG TGCTCCAATG GGGAGAAATG CCCACGTCTG TGGCCTACAT TCATTCCAAT 
CAGATAATGG GCTGGGGCGA GAAAGCTATT GAGATCCGGT CAGTGGAAAC AGGACATTTG 
GATGGAGTAT TTATGCATAA GCGAGCTCAA AGGTTAAAGT TTCTATGTGA AAGAAATGAT 
AAGGTATTTT TTGCATCCGT GCGATCTGGA GGAAGTAGCC AAGTGTTTTT CATGACCCTC 
AACAGAAATT CCATGATGAA CTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851243.1
CDS	365..4351
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_024995475.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995475.1

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGGAGGCTC TGAATGTGAG CTCTCACCGC 
GTCGAGATGC CACGCCAGAA CTCAGATCCC ACCTCTGAAA ACCCACCTCT CCCCACTCGC 
ATTGAGAAGT TTGACCGAAG CTCTTGGTTA CGACAGGAAG AAGACATTCC ACCAAAGGTG 
CCTCAAAGAA CAACTTCTAT ATCCCCAGCA TTAGCAAGGA AGAATTCTCC TGGAAATGGT 
AGTGCTCTGG GACCCAGACT AGGATCTCAA CCCATCAGAG CAAGCAACCC CGACCTGCGG 
AGAACCGAGC CCGTCTTGGA GAGCCCCTTG CAGAGGACGA GCAGTGGCAG TTCTTCCAGC 
TCCAGCACCC CCAGCTCCCA GCCCAGCTCC CAAGGAGGCT CTCAGCCTGG GTCACAAGCA 
GGATCCAGCG AGCGGACCCG GGTTCGAGCC AACAGTAAGT CGGAAGGATC ACCTGTGCTC 
CCCCATGAGC CCTCCAAGGT GAAACCAGAA GAATCCAGGG AAATTACCCG ACCAAGTCGC 
CCAGCTAGCT ATAAGAAAGC TATAGATGAG GATCTGACGG CATTGGCCAA AGAATTAAGA 
GAACTTCGGA TTGAAGAAAC AAACCGCCCA CTGAAGAAGG TAACAGATTA CTCCTCCTCC 
AGTGAGGAGT CAGAAAGCAG TGATGAAGAG GAGGAAGATG GAGAGAGTGA GACCCAGGAT 
GGGACGGTGG CTGTCAGCGA CATACCCAGA CTGATACCCA CAGGAGCCCC CGGGAGCGCT 
GAACAGTACA ATGTGGGAAT GGTCGGCACA CACGCGCTGG AGACCTCTCA TGCAGATACT 
TTCGGCAGCA GTATTTCAAG AGAAGGAACC TTGATGATAA GAGAACCGTC GGGAGAGAAG 
AAGCGACCTG GCCACAGTGA CAGCAATGGC TTTGCCGGCC ACATCAACCT CCCGGACCTG 
GTACAGCAGA GCCATTCCCC TGCGGGGACC CCCACCGAGG GCCTGGGGCG CGTCTCCACC 
CACCCCCAGG AAATGGACGC CGGGACTGAA TATGGCCTGG GGAGCAGCAC CAAAGCCTCC 
TTCACCCCCT TTGTGGACCC CAGGGTATAC CAGACATCTC CCACTGATGA AGATGAAGAG 
GATGAGGAAT CATCAGCTGC GGCTCTGTTT ACTAGCGAAC TTCTTAGGCA AGAACAGGCC 
AAACTAAATG AAGCAAGAAA GATTTCAGTG GTAAATGTAA ACCCAACCAA CATTCGGCCT 
CATAGCGACA CACCGGAGAT CAGAAAATAT AAGAAGCGAT TCAATTCAGA AATACTTTGT 
GCAGCTCTGT GGGGTGTAAA CCTCCTGGTG GGAACCGAAA ATGGCCTGAT GCTTTTGGAC 
CGAAGTGGGC AAGGCAAAGT TTATAATCTG ATCAACCGGA GGCGATTTCA GCAGATGGAT 
GTGCTCGAGG GACTGAATGT CCTCGTGACA ATATCAGGAA AGAAGAATAA GTTACGAGTT 
TACTATCTTT CATGGTTAAG AAACAGAATA CTACACAATG ACCCAGAAGT AGAAAAGAAA 
CAAGGCTGGA TCACTGTGGG AGACTTGGAA GGCTGTATAC ATTACAAAGT TGTTAAATAT 
GAAAGGATCA AATTCTTGGT GATTGCCTTA AAGAATGCTG TGGAGATATA TGCTTGGGCT 
CCTAAACCGT ATCATAAGTT CATGGCATTT AAGTCTTTTG CAGATCTCCA GCACAAGCCA 
CTGCTTGTTG ATCTCACAGT AGAAGAAGGT CAAAGATTAA AGGTTATTTT TGGTTCACAC 
ACTGGTTTCC ATGTAATTGA TGTTGATTCA GGAAACTCTT ATGATATCTA CATACCATCT 
CATATTCAGG GCAATATCAC TCCTCATGCC ATTGTCATCT TGCCTAAGAC AGATGGAATG 
GAAATGCTTG TTTGCTATGA GGATGAGGGG GTGTATGTAA ACACCTACGG CCGGATAACT 
AAGGATGTGG TGCTCCAATG GGGAGAAATG CCCACGTCTG TGGCCTACAT TCATTCCAAT 
CAGATAATGG GCTGGGGCGA GAAAGCTATT GAGATCCGGT CAGTGGAAAC AGGACATTTG 
GATGGAGTAT TTATGCATAA GCGAGCTCAA AGGTTAAAGT TTCTATGTGA AAGAAATGAT 
AAGGTATTTT TTGCATCCGT GCGATCTGGA GGAAGTAGCC AAGTGTTTTT CATGACCCTC 
AACAGAAATT CCATGATGAA CTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_024995482.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851250.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa219205
Clone ID Related Accession (Same CDS sequence)	XM_024995482.1
Accession Version	XM_024995482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ATATGGCCTG GGGAGCAGCA CCAAAGCCTC CTTCACCCCC 
TTTGTGGACC CCAGGGTATA CCAGACATCT CCCACTGATG AAGATGAAGA GGATGAGGAA 
TCATCAGCTG CGGCTCTGTT TACTAGCGAA CTTCTTAGGC AAGAACAGGC CAAACTAAAT 
GAAGCAAGAA AGATTTCAGT GGTAAATGTA AACCCAACCA ACATTCGGCC TCATAGCGAC 
ACACCGGAGA TCAGAAAATA TAAGAAGCGA TTCAATTCAG AAATACTTTG TGCAGCTCTG 
TGGGGTGTAA ACCTCCTGGT GGGAACCGAA AATGGCCTGA TGCTTTTGGA CCGAAGTGGG 
CAAGGCAAAG TTTATAATCT GATCAACCGG AGGCGATTTC AGCAGATGGA TGTGCTCGAG 
GGACTGAATG TCCTCGTGAC AATATCAGGA AAGAAGAATA AGTTACGAGT TTACTATCTT 
TCATGGTTAA GAAACAGAAT ACTACACAAT GACCCAGAAG TAGAAAAGAA ACAAGGCTGG 
ATCACTGTGG GAGACTTGGA AGGCTGTATA CATTACAAAG TTGTTAAATA TGAAAGGATC 
AAATTCTTGG TGATTGCCTT AAAGAATGCT GTGGAGATAT ATGCTTGGGC TCCTAAACCG 
TATCATAAGT TCATGGCATT TAAGTCTTTT GCAGATCTCC AGCACAAGCC ACTGCTTGTT 
GATCTCACAG TAGAAGAAGG TCAAAGATTA AAGGTTATTT TTGGTTCACA CACTGGTTTC 
CATGTAATTG ATGTTGATTC AGGAAACTCT TATGATATCT ACATACCATC TCATATTCAG 
GGCAATATCA CTCCTCATGC CATTGTCATC TTGCCTAAGA CAGATGGAAT GGAAATGCTT 
GTTTGCTATG AGGATGAGGG GGTGTATGTA AACACCTACG GCCGGATAAC TAAGGATGTG 
GTGCTCCAAT GGGGAGAAAT GCCCACGTCT GTGGCCTACA TTCATTCCAA TCAGATAATG 
GGCTGGGGCG AGAAAGCTAT TGAGATCCGG TCAGTGGAAA CAGGACATTT GGATGGAGTA 
TTTATGCATA AGCGAGCTCA AAGGTTAAAG TTTCTATGTG AAAGAAATGA TAAGGTATTT 
TTTGCATCCG TGCGATCTGG AGGAAGTAGC CAAGTGTTTT TCATGACCCT CAACAGAAAT 
TCCATGATGA ACTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851250.1
CDS	365..4282
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_024995482.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995482.1

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ATATGGCCTG GGGAGCAGCA CCAAAGCCTC CTTCACCCCC 
TTTGTGGACC CCAGGGTATA CCAGACATCT CCCACTGATG AAGATGAAGA GGATGAGGAA 
TCATCAGCTG CGGCTCTGTT TACTAGCGAA CTTCTTAGGC AAGAACAGGC CAAACTAAAT 
GAAGCAAGAA AGATTTCAGT GGTAAATGTA AACCCAACCA ACATTCGGCC TCATAGCGAC 
ACACCGGAGA TCAGAAAATA TAAGAAGCGA TTCAATTCAG AAATACTTTG TGCAGCTCTG 
TGGGGTGTAA ACCTCCTGGT GGGAACCGAA AATGGCCTGA TGCTTTTGGA CCGAAGTGGG 
CAAGGCAAAG TTTATAATCT GATCAACCGG AGGCGATTTC AGCAGATGGA TGTGCTCGAG 
GGACTGAATG TCCTCGTGAC AATATCAGGA AAGAAGAATA AGTTACGAGT TTACTATCTT 
TCATGGTTAA GAAACAGAAT ACTACACAAT GACCCAGAAG TAGAAAAGAA ACAAGGCTGG 
ATCACTGTGG GAGACTTGGA AGGCTGTATA CATTACAAAG TTGTTAAATA TGAAAGGATC 
AAATTCTTGG TGATTGCCTT AAAGAATGCT GTGGAGATAT ATGCTTGGGC TCCTAAACCG 
TATCATAAGT TCATGGCATT TAAGTCTTTT GCAGATCTCC AGCACAAGCC ACTGCTTGTT 
GATCTCACAG TAGAAGAAGG TCAAAGATTA AAGGTTATTT TTGGTTCACA CACTGGTTTC 
CATGTAATTG ATGTTGATTC AGGAAACTCT TATGATATCT ACATACCATC TCATATTCAG 
GGCAATATCA CTCCTCATGC CATTGTCATC TTGCCTAAGA CAGATGGAAT GGAAATGCTT 
GTTTGCTATG AGGATGAGGG GGTGTATGTA AACACCTACG GCCGGATAAC TAAGGATGTG 
GTGCTCCAAT GGGGAGAAAT GCCCACGTCT GTGGCCTACA TTCATTCCAA TCAGATAATG 
GGCTGGGGCG AGAAAGCTAT TGAGATCCGG TCAGTGGAAA CAGGACATTT GGATGGAGTA 
TTTATGCATA AGCGAGCTCA AAGGTTAAAG TTTCTATGTG AAAGAAATGA TAAGGTATTT 
TTTGCATCCG TGCGATCTGG AGGAAGTAGC CAAGTGTTTT TCATGACCCT CAACAGAAAT 
TCCATGATGA ACTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_003585673.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003585721.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X3, mRNA.

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CloneID	OBa02614
Clone ID Related Accession (Same CDS sequence)	XM_003585673.4 , XM_003585673.3 , XM_003581730.3
Accession Version	XM_003585673.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003585673.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTAGCTA TAAGAAAGCT ATAGATGAGG ATCTGACGGC ATTGGCCAAA 
GAATTAAGAG AACTTCGGAT TGAAGAAACA AACCGCCCAC TGAAGAAGGT AACAGATTAC 
TCCTCCTCCA GTGAGGAGTC AGAAAGCAGT GATGAAGAGG AGGAAGATGG AGAGAGTGAG 
ACCCAGGATG GGACGGTGGC TGTCAGCGAC ATACCCAGAC TGATACCCAC AGGAGCCCCC 
GGGAGCGCTG AACAGTACAA TGTGGGAATG GTCGGCACAC ACGCGCTGGA GACCTCTCAT 
GCAGATACTT TCGGCAGCAG TATTTCAAGA GAAGGAACCT TGATGATAAG AGAACCGTCG 
GGAGAGAAGA AGCGACCTGG CCACAGTGAC AGCAATGGCT TTGCCGGCCA CATCAACCTC 
CCGGACCTGG TACAGCAGAG CCATTCCCCT GCGGGGACCC CCACCGAGGG CCTGGGGCGC 
GTCTCCACCC ACCCCCAGGA AATGGACGCC GGGACTGAAT ATGGCCTGGG GAGCAGCACC 
AAAGCCTCCT TCACCCCCTT TGTGGACCCC AGGGTATACC AGACATCTCC CACTGATGAA 
GATGAAGAGG ATGAGGAATC ATCAGCTGCG GCTCTGTTTA CTAGCGAACT TCTTAGGCAA 
GAACAGGCCA AACTAAATGA AGCAAGAAAG ATTTCAGTGG TAAATGTAAA CCCAACCAAC 
ATTCGGCCTC ATAGCGACAC ACCGGAGATC AGAAAATATA AGAAGCGATT CAATTCAGAA 
ATACTTTGTG CAGCTCTGTG GGGTGTAAAC CTCCTGGTGG GAACCGAAAA TGGCCTGATG 
CTTTTGGACC GAAGTGGGCA AGGCAAAGTT TATAATCTGA TCAACCGGAG GCGATTTCAG 
CAGATGGATG TGCTCGAGGG ACTGAATGTC CTCGTGACAA TATCAGGAAA GAAGAATAAG 
TTACGAGTTT ACTATCTTTC ATGGTTAAGA AACAGAATAC TACACAATGA CCCAGAAGTA 
GAAAAGAAAC AAGGCTGGAT CACTGTGGGA GACTTGGAAG GCTGTATACA TTACAAAGTT 
GTTAAATATG AAAGGATCAA ATTCTTGGTG ATTGCCTTAA AGAATGCTGT GGAGATATAT 
GCTTGGGCTC CTAAACCGTA TCATAAGTTC ATGGCATTTA AGTCTTTTGC AGATCTCCAG 
CACAAGCCAC TGCTTGTTGA TCTCACAGTA GAAGAAGGTC AAAGATTAAA GGTTATTTTT 
GGTTCACACA CTGGTTTCCA TGTAATTGAT GTTGATTCAG GAAACTCTTA TGATATCTAC 
ATACCATCTC ATATTCAGGG CAATATCACT CCTCATGCCA TTGTCATCTT GCCTAAGACA 
GATGGAATGG AAATGCTTGT TTGCTATGAG GATGAGGGGG TGTATGTAAA CACCTACGGC 
CGGATAACTA AGGATGTGGT GCTCCAATGG GGAGAAATGC CCACGTCTGT GGCCTACATT 
CATTCCAATC AGATAATGGG CTGGGGCGAG AAAGCTATTG AGATCCGGTC AGTGGAAACA 
GGACATTTGG ATGGAGTATT TATGCATAAG CGAGCTCAAA GGTTAAAGTT TCTATGTGAA 
AGAAATGATA AGGTATTTTT TGCATCCGTG CGATCTGGAG GAAGTAGCCA AGTGTTTTTC 
ATGACCCTCA ACAGAAATTC CATGATGAAC TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003585721.1
CDS	365..4360
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_003585673.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003585673.4

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTAGCTA TAAGAAAGCT ATAGATGAGG ATCTGACGGC ATTGGCCAAA 
GAATTAAGAG AACTTCGGAT TGAAGAAACA AACCGCCCAC TGAAGAAGGT AACAGATTAC 
TCCTCCTCCA GTGAGGAGTC AGAAAGCAGT GATGAAGAGG AGGAAGATGG AGAGAGTGAG 
ACCCAGGATG GGACGGTGGC TGTCAGCGAC ATACCCAGAC TGATACCCAC AGGAGCCCCC 
GGGAGCGCTG AACAGTACAA TGTGGGAATG GTCGGCACAC ACGCGCTGGA GACCTCTCAT 
GCAGATACTT TCGGCAGCAG TATTTCAAGA GAAGGAACCT TGATGATAAG AGAACCGTCG 
GGAGAGAAGA AGCGACCTGG CCACAGTGAC AGCAATGGCT TTGCCGGCCA CATCAACCTC 
CCGGACCTGG TACAGCAGAG CCATTCCCCT GCGGGGACCC CCACCGAGGG CCTGGGGCGC 
GTCTCCACCC ACCCCCAGGA AATGGACGCC GGGACTGAAT ATGGCCTGGG GAGCAGCACC 
AAAGCCTCCT TCACCCCCTT TGTGGACCCC AGGGTATACC AGACATCTCC CACTGATGAA 
GATGAAGAGG ATGAGGAATC ATCAGCTGCG GCTCTGTTTA CTAGCGAACT TCTTAGGCAA 
GAACAGGCCA AACTAAATGA AGCAAGAAAG ATTTCAGTGG TAAATGTAAA CCCAACCAAC 
ATTCGGCCTC ATAGCGACAC ACCGGAGATC AGAAAATATA AGAAGCGATT CAATTCAGAA 
ATACTTTGTG CAGCTCTGTG GGGTGTAAAC CTCCTGGTGG GAACCGAAAA TGGCCTGATG 
CTTTTGGACC GAAGTGGGCA AGGCAAAGTT TATAATCTGA TCAACCGGAG GCGATTTCAG 
CAGATGGATG TGCTCGAGGG ACTGAATGTC CTCGTGACAA TATCAGGAAA GAAGAATAAG 
TTACGAGTTT ACTATCTTTC ATGGTTAAGA AACAGAATAC TACACAATGA CCCAGAAGTA 
GAAAAGAAAC AAGGCTGGAT CACTGTGGGA GACTTGGAAG GCTGTATACA TTACAAAGTT 
GTTAAATATG AAAGGATCAA ATTCTTGGTG ATTGCCTTAA AGAATGCTGT GGAGATATAT 
GCTTGGGCTC CTAAACCGTA TCATAAGTTC ATGGCATTTA AGTCTTTTGC AGATCTCCAG 
CACAAGCCAC TGCTTGTTGA TCTCACAGTA GAAGAAGGTC AAAGATTAAA GGTTATTTTT 
GGTTCACACA CTGGTTTCCA TGTAATTGAT GTTGATTCAG GAAACTCTTA TGATATCTAC 
ATACCATCTC ATATTCAGGG CAATATCACT CCTCATGCCA TTGTCATCTT GCCTAAGACA 
GATGGAATGG AAATGCTTGT TTGCTATGAG GATGAGGGGG TGTATGTAAA CACCTACGGC 
CGGATAACTA AGGATGTGGT GCTCCAATGG GGAGAAATGC CCACGTCTGT GGCCTACATT 
CATTCCAATC AGATAATGGG CTGGGGCGAG AAAGCTATTG AGATCCGGTC AGTGGAAACA 
GGACATTTGG ATGGAGTATT TATGCATAAG CGAGCTCAAA GGTTAAAGTT TCTATGTGAA 
AGAAATGATA AGGTATTTTT TGCATCCGTG CGATCTGGAG GAAGTAGCCA AGTGTTTTTC 
ATGACCCTCA ACAGAAATTC CATGATGAAC TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_003585673.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003585721.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X8, mRNA.

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CloneID	OBa02614
Clone ID Related Accession (Same CDS sequence)	XM_003585673.4 , XM_003585673.3 , XM_003581730.3
Accession Version	XM_003585673.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_003585673.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTAGCTA TAAGAAAGCT ATAGATGAGG ATCTGACGGC ATTGGCCAAA 
GAATTAAGAG AACTTCGGAT TGAAGAAACA AACCGCCCAC TGAAGAAGGT AACAGATTAC 
TCCTCCTCCA GTGAGGAGTC AGAAAGCAGT GATGAAGAGG AGGAAGATGG AGAGAGTGAG 
ACCCAGGATG GGACGGTGGC TGTCAGCGAC ATACCCAGAC TGATACCCAC AGGAGCCCCC 
GGGAGCGCTG AACAGTACAA TGTGGGAATG GTCGGCACAC ACGCGCTGGA GACCTCTCAT 
GCAGATACTT TCGGCAGCAG TATTTCAAGA GAAGGAACCT TGATGATAAG AGAACCGTCG 
GGAGAGAAGA AGCGACCTGG CCACAGTGAC AGCAATGGCT TTGCCGGCCA CATCAACCTC 
CCGGACCTGG TACAGCAGAG CCATTCCCCT GCGGGGACCC CCACCGAGGG CCTGGGGCGC 
GTCTCCACCC ACCCCCAGGA AATGGACGCC GGGACTGAAT ATGGCCTGGG GAGCAGCACC 
AAAGCCTCCT TCACCCCCTT TGTGGACCCC AGGGTATACC AGACATCTCC CACTGATGAA 
GATGAAGAGG ATGAGGAATC ATCAGCTGCG GCTCTGTTTA CTAGCGAACT TCTTAGGCAA 
GAACAGGCCA AACTAAATGA AGCAAGAAAG ATTTCAGTGG TAAATGTAAA CCCAACCAAC 
ATTCGGCCTC ATAGCGACAC ACCGGAGATC AGAAAATATA AGAAGCGATT CAATTCAGAA 
ATACTTTGTG CAGCTCTGTG GGGTGTAAAC CTCCTGGTGG GAACCGAAAA TGGCCTGATG 
CTTTTGGACC GAAGTGGGCA AGGCAAAGTT TATAATCTGA TCAACCGGAG GCGATTTCAG 
CAGATGGATG TGCTCGAGGG ACTGAATGTC CTCGTGACAA TATCAGGAAA GAAGAATAAG 
TTACGAGTTT ACTATCTTTC ATGGTTAAGA AACAGAATAC TACACAATGA CCCAGAAGTA 
GAAAAGAAAC AAGGCTGGAT CACTGTGGGA GACTTGGAAG GCTGTATACA TTACAAAGTT 
GTTAAATATG AAAGGATCAA ATTCTTGGTG ATTGCCTTAA AGAATGCTGT GGAGATATAT 
GCTTGGGCTC CTAAACCGTA TCATAAGTTC ATGGCATTTA AGTCTTTTGC AGATCTCCAG 
CACAAGCCAC TGCTTGTTGA TCTCACAGTA GAAGAAGGTC AAAGATTAAA GGTTATTTTT 
GGTTCACACA CTGGTTTCCA TGTAATTGAT GTTGATTCAG GAAACTCTTA TGATATCTAC 
ATACCATCTC ATATTCAGGG CAATATCACT CCTCATGCCA TTGTCATCTT GCCTAAGACA 
GATGGAATGG AAATGCTTGT TTGCTATGAG GATGAGGGGG TGTATGTAAA CACCTACGGC 
CGGATAACTA AGGATGTGGT GCTCCAATGG GGAGAAATGC CCACGTCTGT GGCCTACATT 
CATTCCAATC AGATAATGGG CTGGGGCGAG AAAGCTATTG AGATCCGGTC AGTGGAAACA 
GGACATTTGG ATGGAGTATT TATGCATAAG CGAGCTCAAA GGTTAAAGTT TCTATGTGAA 
AGAAATGATA AGGTATTTTT TGCATCCGTG CGATCTGGAG GAAGTAGCCA AGTGTTTTTC 
ATGACCCTCA ACAGAAATTC CATGATGAAC TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003585721.1
CDS	341..4336
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_003585673.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003585673.3

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTAGCTA TAAGAAAGCT ATAGATGAGG ATCTGACGGC ATTGGCCAAA 
GAATTAAGAG AACTTCGGAT TGAAGAAACA AACCGCCCAC TGAAGAAGGT AACAGATTAC 
TCCTCCTCCA GTGAGGAGTC AGAAAGCAGT GATGAAGAGG AGGAAGATGG AGAGAGTGAG 
ACCCAGGATG GGACGGTGGC TGTCAGCGAC ATACCCAGAC TGATACCCAC AGGAGCCCCC 
GGGAGCGCTG AACAGTACAA TGTGGGAATG GTCGGCACAC ACGCGCTGGA GACCTCTCAT 
GCAGATACTT TCGGCAGCAG TATTTCAAGA GAAGGAACCT TGATGATAAG AGAACCGTCG 
GGAGAGAAGA AGCGACCTGG CCACAGTGAC AGCAATGGCT TTGCCGGCCA CATCAACCTC 
CCGGACCTGG TACAGCAGAG CCATTCCCCT GCGGGGACCC CCACCGAGGG CCTGGGGCGC 
GTCTCCACCC ACCCCCAGGA AATGGACGCC GGGACTGAAT ATGGCCTGGG GAGCAGCACC 
AAAGCCTCCT TCACCCCCTT TGTGGACCCC AGGGTATACC AGACATCTCC CACTGATGAA 
GATGAAGAGG ATGAGGAATC ATCAGCTGCG GCTCTGTTTA CTAGCGAACT TCTTAGGCAA 
GAACAGGCCA AACTAAATGA AGCAAGAAAG ATTTCAGTGG TAAATGTAAA CCCAACCAAC 
ATTCGGCCTC ATAGCGACAC ACCGGAGATC AGAAAATATA AGAAGCGATT CAATTCAGAA 
ATACTTTGTG CAGCTCTGTG GGGTGTAAAC CTCCTGGTGG GAACCGAAAA TGGCCTGATG 
CTTTTGGACC GAAGTGGGCA AGGCAAAGTT TATAATCTGA TCAACCGGAG GCGATTTCAG 
CAGATGGATG TGCTCGAGGG ACTGAATGTC CTCGTGACAA TATCAGGAAA GAAGAATAAG 
TTACGAGTTT ACTATCTTTC ATGGTTAAGA AACAGAATAC TACACAATGA CCCAGAAGTA 
GAAAAGAAAC AAGGCTGGAT CACTGTGGGA GACTTGGAAG GCTGTATACA TTACAAAGTT 
GTTAAATATG AAAGGATCAA ATTCTTGGTG ATTGCCTTAA AGAATGCTGT GGAGATATAT 
GCTTGGGCTC CTAAACCGTA TCATAAGTTC ATGGCATTTA AGTCTTTTGC AGATCTCCAG 
CACAAGCCAC TGCTTGTTGA TCTCACAGTA GAAGAAGGTC AAAGATTAAA GGTTATTTTT 
GGTTCACACA CTGGTTTCCA TGTAATTGAT GTTGATTCAG GAAACTCTTA TGATATCTAC 
ATACCATCTC ATATTCAGGG CAATATCACT CCTCATGCCA TTGTCATCTT GCCTAAGACA 
GATGGAATGG AAATGCTTGT TTGCTATGAG GATGAGGGGG TGTATGTAAA CACCTACGGC 
CGGATAACTA AGGATGTGGT GCTCCAATGG GGAGAAATGC CCACGTCTGT GGCCTACATT 
CATTCCAATC AGATAATGGG CTGGGGCGAG AAAGCTATTG AGATCCGGTC AGTGGAAACA 
GGACATTTGG ATGGAGTATT TATGCATAAG CGAGCTCAAA GGTTAAAGTT TCTATGTGAA 
AGAAATGATA AGGTATTTTT TGCATCCGTG CGATCTGGAG GAAGTAGCCA AGTGTTTTTC 
ATGACCCTCA ACAGAAATTC CATGATGAAC TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_003581730.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003581778.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa02614
Clone ID Related Accession (Same CDS sequence)	XM_003585673.4 , XM_003585673.3 , XM_003581730.3
Accession Version	XM_003581730.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644547.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_003581730.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTAGCTA TAAGAAAGCT ATAGATGAGG ATCTGACGGC ATTGGCCAAA 
GAATTAAGAG AACTTCGGAT TGAAGAAACA AACCGCCCAC TGAAGAAGGT AACAGATTAC 
TCCTCCTCCA GTGAGGAGTC AGAAAGCAGT GATGAAGAGG AGGAAGATGG AGAGAGTGAG 
ACCCAGGATG GGACGGTGGC TGTCAGCGAC ATACCCAGAC TGATACCCAC AGGAGCCCCC 
GGGAGCGCTG AACAGTACAA TGTGGGAATG GTCGGCACAC ACGCGCTGGA GACCTCTCAT 
GCAGATACTT TCGGCAGCAG TATTTCAAGA GAAGGAACCT TGATGATAAG AGAACCGTCG 
GGAGAGAAGA AGCGACCTGG CCACAGTGAC AGCAATGGCT TTGCCGGCCA CATCAACCTC 
CCGGACCTGG TACAGCAGAG CCATTCCCCT GCGGGGACCC CCACCGAGGG CCTGGGGCGC 
GTCTCCACCC ACCCCCAGGA AATGGACGCC GGGACTGAAT ATGGCCTGGG GAGCAGCACC 
AAAGCCTCCT TCACCCCCTT TGTGGACCCC AGGGTATACC AGACATCTCC CACTGATGAA 
GATGAAGAGG ATGAGGAATC ATCAGCTGCG GCTCTGTTTA CTAGCGAACT TCTTAGGCAA 
GAACAGGCCA AACTAAATGA AGCAAGAAAG ATTTCAGTGG TAAATGTAAA CCCAACCAAC 
ATTCGGCCTC ATAGCGACAC ACCGGAGATC AGAAAATATA AGAAGCGATT CAATTCAGAA 
ATACTTTGTG CAGCTCTGTG GGGTGTAAAC CTCCTGGTGG GAACCGAAAA TGGCCTGATG 
CTTTTGGACC GAAGTGGGCA AGGCAAAGTT TATAATCTGA TCAACCGGAG GCGATTTCAG 
CAGATGGATG TGCTCGAGGG ACTGAATGTC CTCGTGACAA TATCAGGAAA GAAGAATAAG 
TTACGAGTTT ACTATCTTTC ATGGTTAAGA AACAGAATAC TACACAATGA CCCAGAAGTA 
GAAAAGAAAC AAGGCTGGAT CACTGTGGGA GACTTGGAAG GCTGTATACA TTACAAAGTT 
GTTAAATATG AAAGGATCAA ATTCTTGGTG ATTGCCTTAA AGAATGCTGT GGAGATATAT 
GCTTGGGCTC CTAAACCGTA TCATAAGTTC ATGGCATTTA AGTCTTTTGC AGATCTCCAG 
CACAAGCCAC TGCTTGTTGA TCTCACAGTA GAAGAAGGTC AAAGATTAAA GGTTATTTTT 
GGTTCACACA CTGGTTTCCA TGTAATTGAT GTTGATTCAG GAAACTCTTA TGATATCTAC 
ATACCATCTC ATATTCAGGG CAATATCACT CCTCATGCCA TTGTCATCTT GCCTAAGACA 
GATGGAATGG AAATGCTTGT TTGCTATGAG GATGAGGGGG TGTATGTAAA CACCTACGGC 
CGGATAACTA AGGATGTGGT GCTCCAATGG GGAGAAATGC CCACGTCTGT GGCCTACATT 
CATTCCAATC AGATAATGGG CTGGGGCGAG AAAGCTATTG AGATCCGGTC AGTGGAAACA 
GGACATTTGG ATGGAGTATT TATGCATAAG CGAGCTCAAA GGTTAAAGTT TCTATGTGAA 
AGAAATGATA AGGTATTTTT TGCATCCGTG CGATCTGGAG GAAGTAGCCA AGTGTTTTTC 
ATGACCCTCA ACAGAAATTC CATGATGAAC TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003581778.1
CDS	341..4336
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_003581730.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003581730.3

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTAGCTA TAAGAAAGCT ATAGATGAGG ATCTGACGGC ATTGGCCAAA 
GAATTAAGAG AACTTCGGAT TGAAGAAACA AACCGCCCAC TGAAGAAGGT AACAGATTAC 
TCCTCCTCCA GTGAGGAGTC AGAAAGCAGT GATGAAGAGG AGGAAGATGG AGAGAGTGAG 
ACCCAGGATG GGACGGTGGC TGTCAGCGAC ATACCCAGAC TGATACCCAC AGGAGCCCCC 
GGGAGCGCTG AACAGTACAA TGTGGGAATG GTCGGCACAC ACGCGCTGGA GACCTCTCAT 
GCAGATACTT TCGGCAGCAG TATTTCAAGA GAAGGAACCT TGATGATAAG AGAACCGTCG 
GGAGAGAAGA AGCGACCTGG CCACAGTGAC AGCAATGGCT TTGCCGGCCA CATCAACCTC 
CCGGACCTGG TACAGCAGAG CCATTCCCCT GCGGGGACCC CCACCGAGGG CCTGGGGCGC 
GTCTCCACCC ACCCCCAGGA AATGGACGCC GGGACTGAAT ATGGCCTGGG GAGCAGCACC 
AAAGCCTCCT TCACCCCCTT TGTGGACCCC AGGGTATACC AGACATCTCC CACTGATGAA 
GATGAAGAGG ATGAGGAATC ATCAGCTGCG GCTCTGTTTA CTAGCGAACT TCTTAGGCAA 
GAACAGGCCA AACTAAATGA AGCAAGAAAG ATTTCAGTGG TAAATGTAAA CCCAACCAAC 
ATTCGGCCTC ATAGCGACAC ACCGGAGATC AGAAAATATA AGAAGCGATT CAATTCAGAA 
ATACTTTGTG CAGCTCTGTG GGGTGTAAAC CTCCTGGTGG GAACCGAAAA TGGCCTGATG 
CTTTTGGACC GAAGTGGGCA AGGCAAAGTT TATAATCTGA TCAACCGGAG GCGATTTCAG 
CAGATGGATG TGCTCGAGGG ACTGAATGTC CTCGTGACAA TATCAGGAAA GAAGAATAAG 
TTACGAGTTT ACTATCTTTC ATGGTTAAGA AACAGAATAC TACACAATGA CCCAGAAGTA 
GAAAAGAAAC AAGGCTGGAT CACTGTGGGA GACTTGGAAG GCTGTATACA TTACAAAGTT 
GTTAAATATG AAAGGATCAA ATTCTTGGTG ATTGCCTTAA AGAATGCTGT GGAGATATAT 
GCTTGGGCTC CTAAACCGTA TCATAAGTTC ATGGCATTTA AGTCTTTTGC AGATCTCCAG 
CACAAGCCAC TGCTTGTTGA TCTCACAGTA GAAGAAGGTC AAAGATTAAA GGTTATTTTT 
GGTTCACACA CTGGTTTCCA TGTAATTGAT GTTGATTCAG GAAACTCTTA TGATATCTAC 
ATACCATCTC ATATTCAGGG CAATATCACT CCTCATGCCA TTGTCATCTT GCCTAAGACA 
GATGGAATGG AAATGCTTGT TTGCTATGAG GATGAGGGGG TGTATGTAAA CACCTACGGC 
CGGATAACTA AGGATGTGGT GCTCCAATGG GGAGAAATGC CCACGTCTGT GGCCTACATT 
CATTCCAATC AGATAATGGG CTGGGGCGAG AAAGCTATTG AGATCCGGTC AGTGGAAACA 
GGACATTTGG ATGGAGTATT TATGCATAAG CGAGCTCAAA GGTTAAAGTT TCTATGTGAA 
AGAAATGATA AGGTATTTTT TGCATCCGTG CGATCTGGAG GAAGTAGCCA AGTGTTTTTC 
ATGACCCTCA ACAGAAATTC CATGATGAAC TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_003585675.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003585723.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa02615
Clone ID Related Accession (Same CDS sequence)	XM_003585675.3 , XM_003585675.4 , XM_003581732.3
Accession Version	XM_003585675.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_003585675.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTGATCT GACGGCATTG GCCAAAGAAT TAAGAGAACT TCGGATTGAA 
GAAACAAACC GCCCACTGAA GAAGGTAACA GATTACTCCT CCTCCAGTGA GGAGTCAGAA 
AGCAGTGATG AAGAGGAGGA AGATGGAGAG AGTGAGACCC AGGATGGGAC GGTGGCTGTC 
AGCGACATAC CCAGACTGAT ACCCACAGGA GCCCCCGGGA GCGCTGAACA GTACAATGTG 
GGAATGGTCG GCACACACGC GCTGGAGACC TCTCATGCAG ATACTTTCGG CAGCAGTATT 
TCAAGAGAAG GAACCTTGAT GATAAGAGAA CCGTCGGGAG AGAAGAAGCG ACCTGGCCAC 
AGTGACAGCA ATGGCTTTGC CGGCCACATC AACCTCCCGG ACCTGGTACA GCAGAGCCAT 
TCCCCTGCGG GGACCCCCAC CGAGGGCCTG GGGCGCGTCT CCACCCACCC CCAGGAAATG 
GACGCCGGGA CTGAATATGG CCTGGGGAGC AGCACCAAAG CCTCCTTCAC CCCCTTTGTG 
GACCCCAGGG TATACCAGAC ATCTCCCACT GATGAAGATG AAGAGGATGA GGAATCATCA 
GCTGCGGCTC TGTTTACTAG CGAACTTCTT AGGCAAGAAC AGGCCAAACT AAATGAAGCA 
AGAAAGATTT CAGTGGTAAA TGTAAACCCA ACCAACATTC GGCCTCATAG CGACACACCG 
GAGATCAGAA AATATAAGAA GCGATTCAAT TCAGAAATAC TTTGTGCAGC TCTGTGGGGT 
GTAAACCTCC TGGTGGGAAC CGAAAATGGC CTGATGCTTT TGGACCGAAG TGGGCAAGGC 
AAAGTTTATA ATCTGATCAA CCGGAGGCGA TTTCAGCAGA TGGATGTGCT CGAGGGACTG 
AATGTCCTCG TGACAATATC AGGAAAGAAG AATAAGTTAC GAGTTTACTA TCTTTCATGG 
TTAAGAAACA GAATACTACA CAATGACCCA GAAGTAGAAA AGAAACAAGG CTGGATCACT 
GTGGGAGACT TGGAAGGCTG TATACATTAC AAAGTTGTTA AATATGAAAG GATCAAATTC 
TTGGTGATTG CCTTAAAGAA TGCTGTGGAG ATATATGCTT GGGCTCCTAA ACCGTATCAT 
AAGTTCATGG CATTTAAGTC TTTTGCAGAT CTCCAGCACA AGCCACTGCT TGTTGATCTC 
ACAGTAGAAG AAGGTCAAAG ATTAAAGGTT ATTTTTGGTT CACACACTGG TTTCCATGTA 
ATTGATGTTG ATTCAGGAAA CTCTTATGAT ATCTACATAC CATCTCATAT TCAGGGCAAT 
ATCACTCCTC ATGCCATTGT CATCTTGCCT AAGACAGATG GAATGGAAAT GCTTGTTTGC 
TATGAGGATG AGGGGGTGTA TGTAAACACC TACGGCCGGA TAACTAAGGA TGTGGTGCTC 
CAATGGGGAG AAATGCCCAC GTCTGTGGCC TACATTCATT CCAATCAGAT AATGGGCTGG 
GGCGAGAAAG CTATTGAGAT CCGGTCAGTG GAAACAGGAC ATTTGGATGG AGTATTTATG 
CATAAGCGAG CTCAAAGGTT AAAGTTTCTA TGTGAAAGAA ATGATAAGGT ATTTTTTGCA 
TCCGTGCGAT CTGGAGGAAG TAGCCAAGTG TTTTTCATGA CCCTCAACAG AAATTCCATG 
ATGAACTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003585723.1
CDS	341..4312
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPADLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_003585675.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003585675.3

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTGATCT GACGGCATTG GCCAAAGAAT TAAGAGAACT TCGGATTGAA 
GAAACAAACC GCCCACTGAA GAAGGTAACA GATTACTCCT CCTCCAGTGA GGAGTCAGAA 
AGCAGTGATG AAGAGGAGGA AGATGGAGAG AGTGAGACCC AGGATGGGAC GGTGGCTGTC 
AGCGACATAC CCAGACTGAT ACCCACAGGA GCCCCCGGGA GCGCTGAACA GTACAATGTG 
GGAATGGTCG GCACACACGC GCTGGAGACC TCTCATGCAG ATACTTTCGG CAGCAGTATT 
TCAAGAGAAG GAACCTTGAT GATAAGAGAA CCGTCGGGAG AGAAGAAGCG ACCTGGCCAC 
AGTGACAGCA ATGGCTTTGC CGGCCACATC AACCTCCCGG ACCTGGTACA GCAGAGCCAT 
TCCCCTGCGG GGACCCCCAC CGAGGGCCTG GGGCGCGTCT CCACCCACCC CCAGGAAATG 
GACGCCGGGA CTGAATATGG CCTGGGGAGC AGCACCAAAG CCTCCTTCAC CCCCTTTGTG 
GACCCCAGGG TATACCAGAC ATCTCCCACT GATGAAGATG AAGAGGATGA GGAATCATCA 
GCTGCGGCTC TGTTTACTAG CGAACTTCTT AGGCAAGAAC AGGCCAAACT AAATGAAGCA 
AGAAAGATTT CAGTGGTAAA TGTAAACCCA ACCAACATTC GGCCTCATAG CGACACACCG 
GAGATCAGAA AATATAAGAA GCGATTCAAT TCAGAAATAC TTTGTGCAGC TCTGTGGGGT 
GTAAACCTCC TGGTGGGAAC CGAAAATGGC CTGATGCTTT TGGACCGAAG TGGGCAAGGC 
AAAGTTTATA ATCTGATCAA CCGGAGGCGA TTTCAGCAGA TGGATGTGCT CGAGGGACTG 
AATGTCCTCG TGACAATATC AGGAAAGAAG AATAAGTTAC GAGTTTACTA TCTTTCATGG 
TTAAGAAACA GAATACTACA CAATGACCCA GAAGTAGAAA AGAAACAAGG CTGGATCACT 
GTGGGAGACT TGGAAGGCTG TATACATTAC AAAGTTGTTA AATATGAAAG GATCAAATTC 
TTGGTGATTG CCTTAAAGAA TGCTGTGGAG ATATATGCTT GGGCTCCTAA ACCGTATCAT 
AAGTTCATGG CATTTAAGTC TTTTGCAGAT CTCCAGCACA AGCCACTGCT TGTTGATCTC 
ACAGTAGAAG AAGGTCAAAG ATTAAAGGTT ATTTTTGGTT CACACACTGG TTTCCATGTA 
ATTGATGTTG ATTCAGGAAA CTCTTATGAT ATCTACATAC CATCTCATAT TCAGGGCAAT 
ATCACTCCTC ATGCCATTGT CATCTTGCCT AAGACAGATG GAATGGAAAT GCTTGTTTGC 
TATGAGGATG AGGGGGTGTA TGTAAACACC TACGGCCGGA TAACTAAGGA TGTGGTGCTC 
CAATGGGGAG AAATGCCCAC GTCTGTGGCC TACATTCATT CCAATCAGAT AATGGGCTGG 
GGCGAGAAAG CTATTGAGAT CCGGTCAGTG GAAACAGGAC ATTTGGATGG AGTATTTATG 
CATAAGCGAG CTCAAAGGTT AAAGTTTCTA TGTGAAAGAA ATGATAAGGT ATTTTTTGCA 
TCCGTGCGAT CTGGAGGAAG TAGCCAAGTG TTTTTCATGA CCCTCAACAG AAATTCCATG 
ATGAACTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_003585675.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003585723.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X5, mRNA.

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CloneID	OBa02615
Clone ID Related Accession (Same CDS sequence)	XM_003585675.3 , XM_003585675.4 , XM_003581732.3
Accession Version	XM_003585675.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003585675.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTGATCT GACGGCATTG GCCAAAGAAT TAAGAGAACT TCGGATTGAA 
GAAACAAACC GCCCACTGAA GAAGGTAACA GATTACTCCT CCTCCAGTGA GGAGTCAGAA 
AGCAGTGATG AAGAGGAGGA AGATGGAGAG AGTGAGACCC AGGATGGGAC GGTGGCTGTC 
AGCGACATAC CCAGACTGAT ACCCACAGGA GCCCCCGGGA GCGCTGAACA GTACAATGTG 
GGAATGGTCG GCACACACGC GCTGGAGACC TCTCATGCAG ATACTTTCGG CAGCAGTATT 
TCAAGAGAAG GAACCTTGAT GATAAGAGAA CCGTCGGGAG AGAAGAAGCG ACCTGGCCAC 
AGTGACAGCA ATGGCTTTGC CGGCCACATC AACCTCCCGG ACCTGGTACA GCAGAGCCAT 
TCCCCTGCGG GGACCCCCAC CGAGGGCCTG GGGCGCGTCT CCACCCACCC CCAGGAAATG 
GACGCCGGGA CTGAATATGG CCTGGGGAGC AGCACCAAAG CCTCCTTCAC CCCCTTTGTG 
GACCCCAGGG TATACCAGAC ATCTCCCACT GATGAAGATG AAGAGGATGA GGAATCATCA 
GCTGCGGCTC TGTTTACTAG CGAACTTCTT AGGCAAGAAC AGGCCAAACT AAATGAAGCA 
AGAAAGATTT CAGTGGTAAA TGTAAACCCA ACCAACATTC GGCCTCATAG CGACACACCG 
GAGATCAGAA AATATAAGAA GCGATTCAAT TCAGAAATAC TTTGTGCAGC TCTGTGGGGT 
GTAAACCTCC TGGTGGGAAC CGAAAATGGC CTGATGCTTT TGGACCGAAG TGGGCAAGGC 
AAAGTTTATA ATCTGATCAA CCGGAGGCGA TTTCAGCAGA TGGATGTGCT CGAGGGACTG 
AATGTCCTCG TGACAATATC AGGAAAGAAG AATAAGTTAC GAGTTTACTA TCTTTCATGG 
TTAAGAAACA GAATACTACA CAATGACCCA GAAGTAGAAA AGAAACAAGG CTGGATCACT 
GTGGGAGACT TGGAAGGCTG TATACATTAC AAAGTTGTTA AATATGAAAG GATCAAATTC 
TTGGTGATTG CCTTAAAGAA TGCTGTGGAG ATATATGCTT GGGCTCCTAA ACCGTATCAT 
AAGTTCATGG CATTTAAGTC TTTTGCAGAT CTCCAGCACA AGCCACTGCT TGTTGATCTC 
ACAGTAGAAG AAGGTCAAAG ATTAAAGGTT ATTTTTGGTT CACACACTGG TTTCCATGTA 
ATTGATGTTG ATTCAGGAAA CTCTTATGAT ATCTACATAC CATCTCATAT TCAGGGCAAT 
ATCACTCCTC ATGCCATTGT CATCTTGCCT AAGACAGATG GAATGGAAAT GCTTGTTTGC 
TATGAGGATG AGGGGGTGTA TGTAAACACC TACGGCCGGA TAACTAAGGA TGTGGTGCTC 
CAATGGGGAG AAATGCCCAC GTCTGTGGCC TACATTCATT CCAATCAGAT AATGGGCTGG 
GGCGAGAAAG CTATTGAGAT CCGGTCAGTG GAAACAGGAC ATTTGGATGG AGTATTTATG 
CATAAGCGAG CTCAAAGGTT AAAGTTTCTA TGTGAAAGAA ATGATAAGGT ATTTTTTGCA 
TCCGTGCGAT CTGGAGGAAG TAGCCAAGTG TTTTTCATGA CCCTCAACAG AAATTCCATG 
ATGAACTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003585723.1
CDS	365..4336
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPADLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_003585675.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003585675.4

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTGATCT GACGGCATTG GCCAAAGAAT TAAGAGAACT TCGGATTGAA 
GAAACAAACC GCCCACTGAA GAAGGTAACA GATTACTCCT CCTCCAGTGA GGAGTCAGAA 
AGCAGTGATG AAGAGGAGGA AGATGGAGAG AGTGAGACCC AGGATGGGAC GGTGGCTGTC 
AGCGACATAC CCAGACTGAT ACCCACAGGA GCCCCCGGGA GCGCTGAACA GTACAATGTG 
GGAATGGTCG GCACACACGC GCTGGAGACC TCTCATGCAG ATACTTTCGG CAGCAGTATT 
TCAAGAGAAG GAACCTTGAT GATAAGAGAA CCGTCGGGAG AGAAGAAGCG ACCTGGCCAC 
AGTGACAGCA ATGGCTTTGC CGGCCACATC AACCTCCCGG ACCTGGTACA GCAGAGCCAT 
TCCCCTGCGG GGACCCCCAC CGAGGGCCTG GGGCGCGTCT CCACCCACCC CCAGGAAATG 
GACGCCGGGA CTGAATATGG CCTGGGGAGC AGCACCAAAG CCTCCTTCAC CCCCTTTGTG 
GACCCCAGGG TATACCAGAC ATCTCCCACT GATGAAGATG AAGAGGATGA GGAATCATCA 
GCTGCGGCTC TGTTTACTAG CGAACTTCTT AGGCAAGAAC AGGCCAAACT AAATGAAGCA 
AGAAAGATTT CAGTGGTAAA TGTAAACCCA ACCAACATTC GGCCTCATAG CGACACACCG 
GAGATCAGAA AATATAAGAA GCGATTCAAT TCAGAAATAC TTTGTGCAGC TCTGTGGGGT 
GTAAACCTCC TGGTGGGAAC CGAAAATGGC CTGATGCTTT TGGACCGAAG TGGGCAAGGC 
AAAGTTTATA ATCTGATCAA CCGGAGGCGA TTTCAGCAGA TGGATGTGCT CGAGGGACTG 
AATGTCCTCG TGACAATATC AGGAAAGAAG AATAAGTTAC GAGTTTACTA TCTTTCATGG 
TTAAGAAACA GAATACTACA CAATGACCCA GAAGTAGAAA AGAAACAAGG CTGGATCACT 
GTGGGAGACT TGGAAGGCTG TATACATTAC AAAGTTGTTA AATATGAAAG GATCAAATTC 
TTGGTGATTG CCTTAAAGAA TGCTGTGGAG ATATATGCTT GGGCTCCTAA ACCGTATCAT 
AAGTTCATGG CATTTAAGTC TTTTGCAGAT CTCCAGCACA AGCCACTGCT TGTTGATCTC 
ACAGTAGAAG AAGGTCAAAG ATTAAAGGTT ATTTTTGGTT CACACACTGG TTTCCATGTA 
ATTGATGTTG ATTCAGGAAA CTCTTATGAT ATCTACATAC CATCTCATAT TCAGGGCAAT 
ATCACTCCTC ATGCCATTGT CATCTTGCCT AAGACAGATG GAATGGAAAT GCTTGTTTGC 
TATGAGGATG AGGGGGTGTA TGTAAACACC TACGGCCGGA TAACTAAGGA TGTGGTGCTC 
CAATGGGGAG AAATGCCCAC GTCTGTGGCC TACATTCATT CCAATCAGAT AATGGGCTGG 
GGCGAGAAAG CTATTGAGAT CCGGTCAGTG GAAACAGGAC ATTTGGATGG AGTATTTATG 
CATAAGCGAG CTCAAAGGTT AAAGTTTCTA TGTGAAAGAA ATGATAAGGT ATTTTTTGCA 
TCCGTGCGAT CTGGAGGAAG TAGCCAAGTG TTTTTCATGA CCCTCAACAG AAATTCCATG 
ATGAACTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_003581732.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003581780.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X9, mRNA.

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CloneID	OBa02615
Clone ID Related Accession (Same CDS sequence)	XM_003585675.3 , XM_003585675.4 , XM_003581732.3
Accession Version	XM_003581732.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644547.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_003581732.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTGATCT GACGGCATTG GCCAAAGAAT TAAGAGAACT TCGGATTGAA 
GAAACAAACC GCCCACTGAA GAAGGTAACA GATTACTCCT CCTCCAGTGA GGAGTCAGAA 
AGCAGTGATG AAGAGGAGGA AGATGGAGAG AGTGAGACCC AGGATGGGAC GGTGGCTGTC 
AGCGACATAC CCAGACTGAT ACCCACAGGA GCCCCCGGGA GCGCTGAACA GTACAATGTG 
GGAATGGTCG GCACACACGC GCTGGAGACC TCTCATGCAG ATACTTTCGG CAGCAGTATT 
TCAAGAGAAG GAACCTTGAT GATAAGAGAA CCGTCGGGAG AGAAGAAGCG ACCTGGCCAC 
AGTGACAGCA ATGGCTTTGC CGGCCACATC AACCTCCCGG ACCTGGTACA GCAGAGCCAT 
TCCCCTGCGG GGACCCCCAC CGAGGGCCTG GGGCGCGTCT CCACCCACCC CCAGGAAATG 
GACGCCGGGA CTGAATATGG CCTGGGGAGC AGCACCAAAG CCTCCTTCAC CCCCTTTGTG 
GACCCCAGGG TATACCAGAC ATCTCCCACT GATGAAGATG AAGAGGATGA GGAATCATCA 
GCTGCGGCTC TGTTTACTAG CGAACTTCTT AGGCAAGAAC AGGCCAAACT AAATGAAGCA 
AGAAAGATTT CAGTGGTAAA TGTAAACCCA ACCAACATTC GGCCTCATAG CGACACACCG 
GAGATCAGAA AATATAAGAA GCGATTCAAT TCAGAAATAC TTTGTGCAGC TCTGTGGGGT 
GTAAACCTCC TGGTGGGAAC CGAAAATGGC CTGATGCTTT TGGACCGAAG TGGGCAAGGC 
AAAGTTTATA ATCTGATCAA CCGGAGGCGA TTTCAGCAGA TGGATGTGCT CGAGGGACTG 
AATGTCCTCG TGACAATATC AGGAAAGAAG AATAAGTTAC GAGTTTACTA TCTTTCATGG 
TTAAGAAACA GAATACTACA CAATGACCCA GAAGTAGAAA AGAAACAAGG CTGGATCACT 
GTGGGAGACT TGGAAGGCTG TATACATTAC AAAGTTGTTA AATATGAAAG GATCAAATTC 
TTGGTGATTG CCTTAAAGAA TGCTGTGGAG ATATATGCTT GGGCTCCTAA ACCGTATCAT 
AAGTTCATGG CATTTAAGTC TTTTGCAGAT CTCCAGCACA AGCCACTGCT TGTTGATCTC 
ACAGTAGAAG AAGGTCAAAG ATTAAAGGTT ATTTTTGGTT CACACACTGG TTTCCATGTA 
ATTGATGTTG ATTCAGGAAA CTCTTATGAT ATCTACATAC CATCTCATAT TCAGGGCAAT 
ATCACTCCTC ATGCCATTGT CATCTTGCCT AAGACAGATG GAATGGAAAT GCTTGTTTGC 
TATGAGGATG AGGGGGTGTA TGTAAACACC TACGGCCGGA TAACTAAGGA TGTGGTGCTC 
CAATGGGGAG AAATGCCCAC GTCTGTGGCC TACATTCATT CCAATCAGAT AATGGGCTGG 
GGCGAGAAAG CTATTGAGAT CCGGTCAGTG GAAACAGGAC ATTTGGATGG AGTATTTATG 
CATAAGCGAG CTCAAAGGTT AAAGTTTCTA TGTGAAAGAA ATGATAAGGT ATTTTTTGCA 
TCCGTGCGAT CTGGAGGAAG TAGCCAAGTG TTTTTCATGA CCCTCAACAG AAATTCCATG 
ATGAACTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003581780.1
CDS	341..4312
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPADLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_003581732.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003581732.3

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTGATCT GACGGCATTG GCCAAAGAAT TAAGAGAACT TCGGATTGAA 
GAAACAAACC GCCCACTGAA GAAGGTAACA GATTACTCCT CCTCCAGTGA GGAGTCAGAA 
AGCAGTGATG AAGAGGAGGA AGATGGAGAG AGTGAGACCC AGGATGGGAC GGTGGCTGTC 
AGCGACATAC CCAGACTGAT ACCCACAGGA GCCCCCGGGA GCGCTGAACA GTACAATGTG 
GGAATGGTCG GCACACACGC GCTGGAGACC TCTCATGCAG ATACTTTCGG CAGCAGTATT 
TCAAGAGAAG GAACCTTGAT GATAAGAGAA CCGTCGGGAG AGAAGAAGCG ACCTGGCCAC 
AGTGACAGCA ATGGCTTTGC CGGCCACATC AACCTCCCGG ACCTGGTACA GCAGAGCCAT 
TCCCCTGCGG GGACCCCCAC CGAGGGCCTG GGGCGCGTCT CCACCCACCC CCAGGAAATG 
GACGCCGGGA CTGAATATGG CCTGGGGAGC AGCACCAAAG CCTCCTTCAC CCCCTTTGTG 
GACCCCAGGG TATACCAGAC ATCTCCCACT GATGAAGATG AAGAGGATGA GGAATCATCA 
GCTGCGGCTC TGTTTACTAG CGAACTTCTT AGGCAAGAAC AGGCCAAACT AAATGAAGCA 
AGAAAGATTT CAGTGGTAAA TGTAAACCCA ACCAACATTC GGCCTCATAG CGACACACCG 
GAGATCAGAA AATATAAGAA GCGATTCAAT TCAGAAATAC TTTGTGCAGC TCTGTGGGGT 
GTAAACCTCC TGGTGGGAAC CGAAAATGGC CTGATGCTTT TGGACCGAAG TGGGCAAGGC 
AAAGTTTATA ATCTGATCAA CCGGAGGCGA TTTCAGCAGA TGGATGTGCT CGAGGGACTG 
AATGTCCTCG TGACAATATC AGGAAAGAAG AATAAGTTAC GAGTTTACTA TCTTTCATGG 
TTAAGAAACA GAATACTACA CAATGACCCA GAAGTAGAAA AGAAACAAGG CTGGATCACT 
GTGGGAGACT TGGAAGGCTG TATACATTAC AAAGTTGTTA AATATGAAAG GATCAAATTC 
TTGGTGATTG CCTTAAAGAA TGCTGTGGAG ATATATGCTT GGGCTCCTAA ACCGTATCAT 
AAGTTCATGG CATTTAAGTC TTTTGCAGAT CTCCAGCACA AGCCACTGCT TGTTGATCTC 
ACAGTAGAAG AAGGTCAAAG ATTAAAGGTT ATTTTTGGTT CACACACTGG TTTCCATGTA 
ATTGATGTTG ATTCAGGAAA CTCTTATGAT ATCTACATAC CATCTCATAT TCAGGGCAAT 
ATCACTCCTC ATGCCATTGT CATCTTGCCT AAGACAGATG GAATGGAAAT GCTTGTTTGC 
TATGAGGATG AGGGGGTGTA TGTAAACACC TACGGCCGGA TAACTAAGGA TGTGGTGCTC 
CAATGGGGAG AAATGCCCAC GTCTGTGGCC TACATTCATT CCAATCAGAT AATGGGCTGG 
GGCGAGAAAG CTATTGAGAT CCGGTCAGTG GAAACAGGAC ATTTGGATGG AGTATTTATG 
CATAAGCGAG CTCAAAGGTT AAAGTTTCTA TGTGAAAGAA ATGATAAGGT ATTTTTTGCA 
TCCGTGCGAT CTGGAGGAAG TAGCCAAGTG TTTTTCATGA CCCTCAACAG AAATTCCATG 
ATGAACTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_005195922.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005195979.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa02611
Clone ID Related Accession (Same CDS sequence)	XM_005195922.2 , XM_005201707.2
Accession Version	XM_005195922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644547.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005195922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ACCGTCGGGA GAGAAGAAGC GACCTGGCCA CAGTGACAGC 
AATGGCTTTG CCGGCCACAT CAACCTCCCG GACCTGGTAC AGCAGAGCCA TTCCCCTGCG 
GGGACCCCCA CCGAGGGCCT GGGGCGCGTC TCCACCCACC CCCAGGAAAT GGACGCCGGG 
ACTGAATATG GCCTGGGGAG CAGCACCAAA GCCTCCTTCA CCCCCTTTGT GGACCCCAGG 
GTATACCAGA CATCTCCCAC TGATGAAGAT GAAGAGGATG AGGAATCATC AGCTGCGGGA 
ACCCAGAGGT ATCCAGGGGC CCTGGGAAAA GCTGTTCCTC CGAGATCTCC AAACTCACCA 
CCTATTTCTC AAAAGCAGTT TCTGGCTCTG TTTACTAGCG AACTTCTTAG GCAAGAACAG 
GCCAAACTAA ATGAAGCAAG AAAGATTTCA GTGGTAAATG TAAACCCAAC CAACATTCGG 
CCTCATAGCG ACACACCGGA GATCAGAAAA TATAAGAAGC GATTCAATTC AGAAATACTT 
TGTGCAGCTC TGTGGGGTGT AAACCTCCTG GTGGGAACCG AAAATGGCCT GATGCTTTTG 
GACCGAAGTG GGCAAGGCAA AGTTTATAAT CTGATCAACC GGAGGCGATT TCAGCAGATG 
GATGTGCTCG AGGGACTGAA TGTCCTCGTG ACAATATCAG GAAAGAAGAA TAAGTTACGA 
GTTTACTATC TTTCATGGTT AAGAAACAGA ATACTACACA ATGACCCAGA AGTAGAAAAG 
AAACAAGGCT GGATCACTGT GGGAGACTTG GAAGGCTGTA TACATTACAA AGTTGTTAAA 
TATGAAAGGA TCAAATTCTT GGTGATTGCC TTAAAGAATG CTGTGGAGAT ATATGCTTGG 
GCTCCTAAAC CGTATCATAA GTTCATGGCA TTTAAGTCTT TTGCAGATCT CCAGCACAAG 
CCACTGCTTG TTGATCTCAC AGTAGAAGAA GGTCAAAGAT TAAAGGTTAT TTTTGGTTCA 
CACACTGGTT TCCATGTAAT TGATGTTGAT TCAGGAAACT CTTATGATAT CTACATACCA 
TCTCATATTC AGGGCAATAT CACTCCTCAT GCCATTGTCA TCTTGCCTAA GACAGATGGA 
ATGGAAATGC TTGTTTGCTA TGAGGATGAG GGGGTGTATG TAAACACCTA CGGCCGGATA 
ACTAAGGATG TGGTGCTCCA ATGGGGAGAA ATGCCCACGT CTGTGGCCTA CATTCATTCC 
AATCAGATAA TGGGCTGGGG CGAGAAAGCT ATTGAGATCC GGTCAGTGGA AACAGGACAT 
TTGGATGGAG TATTTATGCA TAAGCGAGCT CAAAGGTTAA AGTTTCTATG TGAAAGAAAT 
GATAAGGTAT TTTTTGCATC CGTGCGATCT GGAGGAAGTA GCCAAGTGTT TTTCATGACC 
CTCAACAGAA ATTCCATGAT GAACTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005195979.1
CDS	341..4510
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAGTQRYPGALGKAVPPRSPNSPPISQKQFLALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_005195922.2
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005195922.2

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ACCGTCGGGA GAGAAGAAGC GACCTGGCCA CAGTGACAGC 
AATGGCTTTG CCGGCCACAT CAACCTCCCG GACCTGGTAC AGCAGAGCCA TTCCCCTGCG 
GGGACCCCCA CCGAGGGCCT GGGGCGCGTC TCCACCCACC CCCAGGAAAT GGACGCCGGG 
ACTGAATATG GCCTGGGGAG CAGCACCAAA GCCTCCTTCA CCCCCTTTGT GGACCCCAGG 
GTATACCAGA CATCTCCCAC TGATGAAGAT GAAGAGGATG AGGAATCATC AGCTGCGGGA 
ACCCAGAGGT ATCCAGGGGC CCTGGGAAAA GCTGTTCCTC CGAGATCTCC AAACTCACCA 
CCTATTTCTC AAAAGCAGTT TCTGGCTCTG TTTACTAGCG AACTTCTTAG GCAAGAACAG 
GCCAAACTAA ATGAAGCAAG AAAGATTTCA GTGGTAAATG TAAACCCAAC CAACATTCGG 
CCTCATAGCG ACACACCGGA GATCAGAAAA TATAAGAAGC GATTCAATTC AGAAATACTT 
TGTGCAGCTC TGTGGGGTGT AAACCTCCTG GTGGGAACCG AAAATGGCCT GATGCTTTTG 
GACCGAAGTG GGCAAGGCAA AGTTTATAAT CTGATCAACC GGAGGCGATT TCAGCAGATG 
GATGTGCTCG AGGGACTGAA TGTCCTCGTG ACAATATCAG GAAAGAAGAA TAAGTTACGA 
GTTTACTATC TTTCATGGTT AAGAAACAGA ATACTACACA ATGACCCAGA AGTAGAAAAG 
AAACAAGGCT GGATCACTGT GGGAGACTTG GAAGGCTGTA TACATTACAA AGTTGTTAAA 
TATGAAAGGA TCAAATTCTT GGTGATTGCC TTAAAGAATG CTGTGGAGAT ATATGCTTGG 
GCTCCTAAAC CGTATCATAA GTTCATGGCA TTTAAGTCTT TTGCAGATCT CCAGCACAAG 
CCACTGCTTG TTGATCTCAC AGTAGAAGAA GGTCAAAGAT TAAAGGTTAT TTTTGGTTCA 
CACACTGGTT TCCATGTAAT TGATGTTGAT TCAGGAAACT CTTATGATAT CTACATACCA 
TCTCATATTC AGGGCAATAT CACTCCTCAT GCCATTGTCA TCTTGCCTAA GACAGATGGA 
ATGGAAATGC TTGTTTGCTA TGAGGATGAG GGGGTGTATG TAAACACCTA CGGCCGGATA 
ACTAAGGATG TGGTGCTCCA ATGGGGAGAA ATGCCCACGT CTGTGGCCTA CATTCATTCC 
AATCAGATAA TGGGCTGGGG CGAGAAAGCT ATTGAGATCC GGTCAGTGGA AACAGGACAT 
TTGGATGGAG TATTTATGCA TAAGCGAGCT CAAAGGTTAA AGTTTCTATG TGAAAGAAAT 
GATAAGGTAT TTTTTGCATC CGTGCGATCT GGAGGAAGTA GCCAAGTGTT TTTCATGACC 
CTCAACAGAA ATTCCATGAT GAACTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_005201707.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201764.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X1, mRNA.

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CloneID	OBa02611
Clone ID Related Accession (Same CDS sequence)	XM_005195922.2 , XM_005201707.2
Accession Version	XM_005201707.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005201707.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ACCGTCGGGA GAGAAGAAGC GACCTGGCCA CAGTGACAGC 
AATGGCTTTG CCGGCCACAT CAACCTCCCG GACCTGGTAC AGCAGAGCCA TTCCCCTGCG 
GGGACCCCCA CCGAGGGCCT GGGGCGCGTC TCCACCCACC CCCAGGAAAT GGACGCCGGG 
ACTGAATATG GCCTGGGGAG CAGCACCAAA GCCTCCTTCA CCCCCTTTGT GGACCCCAGG 
GTATACCAGA CATCTCCCAC TGATGAAGAT GAAGAGGATG AGGAATCATC AGCTGCGGGA 
ACCCAGAGGT ATCCAGGGGC CCTGGGAAAA GCTGTTCCTC CGAGATCTCC AAACTCACCA 
CCTATTTCTC AAAAGCAGTT TCTGGCTCTG TTTACTAGCG AACTTCTTAG GCAAGAACAG 
GCCAAACTAA ATGAAGCAAG AAAGATTTCA GTGGTAAATG TAAACCCAAC CAACATTCGG 
CCTCATAGCG ACACACCGGA GATCAGAAAA TATAAGAAGC GATTCAATTC AGAAATACTT 
TGTGCAGCTC TGTGGGGTGT AAACCTCCTG GTGGGAACCG AAAATGGCCT GATGCTTTTG 
GACCGAAGTG GGCAAGGCAA AGTTTATAAT CTGATCAACC GGAGGCGATT TCAGCAGATG 
GATGTGCTCG AGGGACTGAA TGTCCTCGTG ACAATATCAG GAAAGAAGAA TAAGTTACGA 
GTTTACTATC TTTCATGGTT AAGAAACAGA ATACTACACA ATGACCCAGA AGTAGAAAAG 
AAACAAGGCT GGATCACTGT GGGAGACTTG GAAGGCTGTA TACATTACAA AGTTGTTAAA 
TATGAAAGGA TCAAATTCTT GGTGATTGCC TTAAAGAATG CTGTGGAGAT ATATGCTTGG 
GCTCCTAAAC CGTATCATAA GTTCATGGCA TTTAAGTCTT TTGCAGATCT CCAGCACAAG 
CCACTGCTTG TTGATCTCAC AGTAGAAGAA GGTCAAAGAT TAAAGGTTAT TTTTGGTTCA 
CACACTGGTT TCCATGTAAT TGATGTTGAT TCAGGAAACT CTTATGATAT CTACATACCA 
TCTCATATTC AGGGCAATAT CACTCCTCAT GCCATTGTCA TCTTGCCTAA GACAGATGGA 
ATGGAAATGC TTGTTTGCTA TGAGGATGAG GGGGTGTATG TAAACACCTA CGGCCGGATA 
ACTAAGGATG TGGTGCTCCA ATGGGGAGAA ATGCCCACGT CTGTGGCCTA CATTCATTCC 
AATCAGATAA TGGGCTGGGG CGAGAAAGCT ATTGAGATCC GGTCAGTGGA AACAGGACAT 
TTGGATGGAG TATTTATGCA TAAGCGAGCT CAAAGGTTAA AGTTTCTATG TGAAAGAAAT 
GATAAGGTAT TTTTTGCATC CGTGCGATCT GGAGGAAGTA GCCAAGTGTT TTTCATGACC 
CTCAACAGAA ATTCCATGAT GAACTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201764.1
CDS	341..4510
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAGTQRYPGALGKAVPPRSPNSPPISQKQFLALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_005201707.2
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201707.2

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ACCGTCGGGA GAGAAGAAGC GACCTGGCCA CAGTGACAGC 
AATGGCTTTG CCGGCCACAT CAACCTCCCG GACCTGGTAC AGCAGAGCCA TTCCCCTGCG 
GGGACCCCCA CCGAGGGCCT GGGGCGCGTC TCCACCCACC CCCAGGAAAT GGACGCCGGG 
ACTGAATATG GCCTGGGGAG CAGCACCAAA GCCTCCTTCA CCCCCTTTGT GGACCCCAGG 
GTATACCAGA CATCTCCCAC TGATGAAGAT GAAGAGGATG AGGAATCATC AGCTGCGGGA 
ACCCAGAGGT ATCCAGGGGC CCTGGGAAAA GCTGTTCCTC CGAGATCTCC AAACTCACCA 
CCTATTTCTC AAAAGCAGTT TCTGGCTCTG TTTACTAGCG AACTTCTTAG GCAAGAACAG 
GCCAAACTAA ATGAAGCAAG AAAGATTTCA GTGGTAAATG TAAACCCAAC CAACATTCGG 
CCTCATAGCG ACACACCGGA GATCAGAAAA TATAAGAAGC GATTCAATTC AGAAATACTT 
TGTGCAGCTC TGTGGGGTGT AAACCTCCTG GTGGGAACCG AAAATGGCCT GATGCTTTTG 
GACCGAAGTG GGCAAGGCAA AGTTTATAAT CTGATCAACC GGAGGCGATT TCAGCAGATG 
GATGTGCTCG AGGGACTGAA TGTCCTCGTG ACAATATCAG GAAAGAAGAA TAAGTTACGA 
GTTTACTATC TTTCATGGTT AAGAAACAGA ATACTACACA ATGACCCAGA AGTAGAAAAG 
AAACAAGGCT GGATCACTGT GGGAGACTTG GAAGGCTGTA TACATTACAA AGTTGTTAAA 
TATGAAAGGA TCAAATTCTT GGTGATTGCC TTAAAGAATG CTGTGGAGAT ATATGCTTGG 
GCTCCTAAAC CGTATCATAA GTTCATGGCA TTTAAGTCTT TTGCAGATCT CCAGCACAAG 
CCACTGCTTG TTGATCTCAC AGTAGAAGAA GGTCAAAGAT TAAAGGTTAT TTTTGGTTCA 
CACACTGGTT TCCATGTAAT TGATGTTGAT TCAGGAAACT CTTATGATAT CTACATACCA 
TCTCATATTC AGGGCAATAT CACTCCTCAT GCCATTGTCA TCTTGCCTAA GACAGATGGA 
ATGGAAATGC TTGTTTGCTA TGAGGATGAG GGGGTGTATG TAAACACCTA CGGCCGGATA 
ACTAAGGATG TGGTGCTCCA ATGGGGAGAA ATGCCCACGT CTGTGGCCTA CATTCATTCC 
AATCAGATAA TGGGCTGGGG CGAGAAAGCT ATTGAGATCC GGTCAGTGGA AACAGGACAT 
TTGGATGGAG TATTTATGCA TAAGCGAGCT CAAAGGTTAA AGTTTCTATG TGAAAGAAAT 
GATAAGGTAT TTTTTGCATC CGTGCGATCT GGAGGAAGTA GCCAAGTGTT TTTCATGACC 
CTCAACAGAA ATTCCATGAT GAACTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_002684922.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002684968.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa02612
Clone ID Related Accession (Same CDS sequence)	XM_002684922.4 , XM_588438.8 , XM_002684922.5
Accession Version	XM_002684922.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_002684922.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ACCGTCGGGA GAGAAGAAGC GACCTGGCCA CAGTGACAGC 
AATGGCTTTG CCGGCCACAT CAACCTCCCG GACCTGGTAC AGCAGAGCCA TTCCCCTGCG 
GGGACCCCCA CCGAGGGCCT GGGGCGCGTC TCCACCCACC CCCAGGAAAT GGACGCCGGG 
ACTGAATATG GCCTGGGGAG CAGCACCAAA GCCTCCTTCA CCCCCTTTGT GGACCCCAGG 
GTATACCAGA CATCTCCCAC TGATGAAGAT GAAGAGGATG AGGAATCATC AGCTGCGGCT 
CTGTTTACTA GCGAACTTCT TAGGCAAGAA CAGGCCAAAC TAAATGAAGC AAGAAAGATT 
TCAGTGGTAA ATGTAAACCC AACCAACATT CGGCCTCATA GCGACACACC GGAGATCAGA 
AAATATAAGA AGCGATTCAA TTCAGAAATA CTTTGTGCAG CTCTGTGGGG TGTAAACCTC 
CTGGTGGGAA CCGAAAATGG CCTGATGCTT TTGGACCGAA GTGGGCAAGG CAAAGTTTAT 
AATCTGATCA ACCGGAGGCG ATTTCAGCAG ATGGATGTGC TCGAGGGACT GAATGTCCTC 
GTGACAATAT CAGGAAAGAA GAATAAGTTA CGAGTTTACT ATCTTTCATG GTTAAGAAAC 
AGAATACTAC ACAATGACCC AGAAGTAGAA AAGAAACAAG GCTGGATCAC TGTGGGAGAC 
TTGGAAGGCT GTATACATTA CAAAGTTGTT AAATATGAAA GGATCAAATT CTTGGTGATT 
GCCTTAAAGA ATGCTGTGGA GATATATGCT TGGGCTCCTA AACCGTATCA TAAGTTCATG 
GCATTTAAGT CTTTTGCAGA TCTCCAGCAC AAGCCACTGC TTGTTGATCT CACAGTAGAA 
GAAGGTCAAA GATTAAAGGT TATTTTTGGT TCACACACTG GTTTCCATGT AATTGATGTT 
GATTCAGGAA ACTCTTATGA TATCTACATA CCATCTCATA TTCAGGGCAA TATCACTCCT 
CATGCCATTG TCATCTTGCC TAAGACAGAT GGAATGGAAA TGCTTGTTTG CTATGAGGAT 
GAGGGGGTGT ATGTAAACAC CTACGGCCGG ATAACTAAGG ATGTGGTGCT CCAATGGGGA 
GAAATGCCCA CGTCTGTGGC CTACATTCAT TCCAATCAGA TAATGGGCTG GGGCGAGAAA 
GCTATTGAGA TCCGGTCAGT GGAAACAGGA CATTTGGATG GAGTATTTAT GCATAAGCGA 
GCTCAAAGGT TAAAGTTTCT ATGTGAAAGA AATGATAAGG TATTTTTTGC ATCCGTGCGA 
TCTGGAGGAA GTAGCCAAGT GTTTTTCATG ACCCTCAACA GAAATTCCAT GATGAACTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002684968.1
CDS	341..4423
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_002684922.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002684922.4

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ACCGTCGGGA GAGAAGAAGC GACCTGGCCA CAGTGACAGC 
AATGGCTTTG CCGGCCACAT CAACCTCCCG GACCTGGTAC AGCAGAGCCA TTCCCCTGCG 
GGGACCCCCA CCGAGGGCCT GGGGCGCGTC TCCACCCACC CCCAGGAAAT GGACGCCGGG 
ACTGAATATG GCCTGGGGAG CAGCACCAAA GCCTCCTTCA CCCCCTTTGT GGACCCCAGG 
GTATACCAGA CATCTCCCAC TGATGAAGAT GAAGAGGATG AGGAATCATC AGCTGCGGCT 
CTGTTTACTA GCGAACTTCT TAGGCAAGAA CAGGCCAAAC TAAATGAAGC AAGAAAGATT 
TCAGTGGTAA ATGTAAACCC AACCAACATT CGGCCTCATA GCGACACACC GGAGATCAGA 
AAATATAAGA AGCGATTCAA TTCAGAAATA CTTTGTGCAG CTCTGTGGGG TGTAAACCTC 
CTGGTGGGAA CCGAAAATGG CCTGATGCTT TTGGACCGAA GTGGGCAAGG CAAAGTTTAT 
AATCTGATCA ACCGGAGGCG ATTTCAGCAG ATGGATGTGC TCGAGGGACT GAATGTCCTC 
GTGACAATAT CAGGAAAGAA GAATAAGTTA CGAGTTTACT ATCTTTCATG GTTAAGAAAC 
AGAATACTAC ACAATGACCC AGAAGTAGAA AAGAAACAAG GCTGGATCAC TGTGGGAGAC 
TTGGAAGGCT GTATACATTA CAAAGTTGTT AAATATGAAA GGATCAAATT CTTGGTGATT 
GCCTTAAAGA ATGCTGTGGA GATATATGCT TGGGCTCCTA AACCGTATCA TAAGTTCATG 
GCATTTAAGT CTTTTGCAGA TCTCCAGCAC AAGCCACTGC TTGTTGATCT CACAGTAGAA 
GAAGGTCAAA GATTAAAGGT TATTTTTGGT TCACACACTG GTTTCCATGT AATTGATGTT 
GATTCAGGAA ACTCTTATGA TATCTACATA CCATCTCATA TTCAGGGCAA TATCACTCCT 
CATGCCATTG TCATCTTGCC TAAGACAGAT GGAATGGAAA TGCTTGTTTG CTATGAGGAT 
GAGGGGGTGT ATGTAAACAC CTACGGCCGG ATAACTAAGG ATGTGGTGCT CCAATGGGGA 
GAAATGCCCA CGTCTGTGGC CTACATTCAT TCCAATCAGA TAATGGGCTG GGGCGAGAAA 
GCTATTGAGA TCCGGTCAGT GGAAACAGGA CATTTGGATG GAGTATTTAT GCATAAGCGA 
GCTCAAAGGT TAAAGTTTCT ATGTGAAAGA AATGATAAGG TATTTTTTGC ATCCGTGCGA 
TCTGGAGGAA GTAGCCAAGT GTTTTTCATG ACCCTCAACA GAAATTCCAT GATGAACTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_588438.8 ) cDNA ORF clone, Bos taurus(cattle) -> XP_588438.5 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa02612
Clone ID Related Accession (Same CDS sequence)	XM_002684922.4 , XM_588438.8 , XM_002684922.5
Accession Version	XM_588438.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644547.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_588438.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ACCGTCGGGA GAGAAGAAGC GACCTGGCCA CAGTGACAGC 
AATGGCTTTG CCGGCCACAT CAACCTCCCG GACCTGGTAC AGCAGAGCCA TTCCCCTGCG 
GGGACCCCCA CCGAGGGCCT GGGGCGCGTC TCCACCCACC CCCAGGAAAT GGACGCCGGG 
ACTGAATATG GCCTGGGGAG CAGCACCAAA GCCTCCTTCA CCCCCTTTGT GGACCCCAGG 
GTATACCAGA CATCTCCCAC TGATGAAGAT GAAGAGGATG AGGAATCATC AGCTGCGGCT 
CTGTTTACTA GCGAACTTCT TAGGCAAGAA CAGGCCAAAC TAAATGAAGC AAGAAAGATT 
TCAGTGGTAA ATGTAAACCC AACCAACATT CGGCCTCATA GCGACACACC GGAGATCAGA 
AAATATAAGA AGCGATTCAA TTCAGAAATA CTTTGTGCAG CTCTGTGGGG TGTAAACCTC 
CTGGTGGGAA CCGAAAATGG CCTGATGCTT TTGGACCGAA GTGGGCAAGG CAAAGTTTAT 
AATCTGATCA ACCGGAGGCG ATTTCAGCAG ATGGATGTGC TCGAGGGACT GAATGTCCTC 
GTGACAATAT CAGGAAAGAA GAATAAGTTA CGAGTTTACT ATCTTTCATG GTTAAGAAAC 
AGAATACTAC ACAATGACCC AGAAGTAGAA AAGAAACAAG GCTGGATCAC TGTGGGAGAC 
TTGGAAGGCT GTATACATTA CAAAGTTGTT AAATATGAAA GGATCAAATT CTTGGTGATT 
GCCTTAAAGA ATGCTGTGGA GATATATGCT TGGGCTCCTA AACCGTATCA TAAGTTCATG 
GCATTTAAGT CTTTTGCAGA TCTCCAGCAC AAGCCACTGC TTGTTGATCT CACAGTAGAA 
GAAGGTCAAA GATTAAAGGT TATTTTTGGT TCACACACTG GTTTCCATGT AATTGATGTT 
GATTCAGGAA ACTCTTATGA TATCTACATA CCATCTCATA TTCAGGGCAA TATCACTCCT 
CATGCCATTG TCATCTTGCC TAAGACAGAT GGAATGGAAA TGCTTGTTTG CTATGAGGAT 
GAGGGGGTGT ATGTAAACAC CTACGGCCGG ATAACTAAGG ATGTGGTGCT CCAATGGGGA 
GAAATGCCCA CGTCTGTGGC CTACATTCAT TCCAATCAGA TAATGGGCTG GGGCGAGAAA 
GCTATTGAGA TCCGGTCAGT GGAAACAGGA CATTTGGATG GAGTATTTAT GCATAAGCGA 
GCTCAAAGGT TAAAGTTTCT ATGTGAAAGA AATGATAAGG TATTTTTTGC ATCCGTGCGA 
TCTGGAGGAA GTAGCCAAGT GTTTTTCATG ACCCTCAACA GAAATTCCAT GATGAACTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_588438.5
CDS	341..4423
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_588438.8
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_588438.8

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ACCGTCGGGA GAGAAGAAGC GACCTGGCCA CAGTGACAGC 
AATGGCTTTG CCGGCCACAT CAACCTCCCG GACCTGGTAC AGCAGAGCCA TTCCCCTGCG 
GGGACCCCCA CCGAGGGCCT GGGGCGCGTC TCCACCCACC CCCAGGAAAT GGACGCCGGG 
ACTGAATATG GCCTGGGGAG CAGCACCAAA GCCTCCTTCA CCCCCTTTGT GGACCCCAGG 
GTATACCAGA CATCTCCCAC TGATGAAGAT GAAGAGGATG AGGAATCATC AGCTGCGGCT 
CTGTTTACTA GCGAACTTCT TAGGCAAGAA CAGGCCAAAC TAAATGAAGC AAGAAAGATT 
TCAGTGGTAA ATGTAAACCC AACCAACATT CGGCCTCATA GCGACACACC GGAGATCAGA 
AAATATAAGA AGCGATTCAA TTCAGAAATA CTTTGTGCAG CTCTGTGGGG TGTAAACCTC 
CTGGTGGGAA CCGAAAATGG CCTGATGCTT TTGGACCGAA GTGGGCAAGG CAAAGTTTAT 
AATCTGATCA ACCGGAGGCG ATTTCAGCAG ATGGATGTGC TCGAGGGACT GAATGTCCTC 
GTGACAATAT CAGGAAAGAA GAATAAGTTA CGAGTTTACT ATCTTTCATG GTTAAGAAAC 
AGAATACTAC ACAATGACCC AGAAGTAGAA AAGAAACAAG GCTGGATCAC TGTGGGAGAC 
TTGGAAGGCT GTATACATTA CAAAGTTGTT AAATATGAAA GGATCAAATT CTTGGTGATT 
GCCTTAAAGA ATGCTGTGGA GATATATGCT TGGGCTCCTA AACCGTATCA TAAGTTCATG 
GCATTTAAGT CTTTTGCAGA TCTCCAGCAC AAGCCACTGC TTGTTGATCT CACAGTAGAA 
GAAGGTCAAA GATTAAAGGT TATTTTTGGT TCACACACTG GTTTCCATGT AATTGATGTT 
GATTCAGGAA ACTCTTATGA TATCTACATA CCATCTCATA TTCAGGGCAA TATCACTCCT 
CATGCCATTG TCATCTTGCC TAAGACAGAT GGAATGGAAA TGCTTGTTTG CTATGAGGAT 
GAGGGGGTGT ATGTAAACAC CTACGGCCGG ATAACTAAGG ATGTGGTGCT CCAATGGGGA 
GAAATGCCCA CGTCTGTGGC CTACATTCAT TCCAATCAGA TAATGGGCTG GGGCGAGAAA 
GCTATTGAGA TCCGGTCAGT GGAAACAGGA CATTTGGATG GAGTATTTAT GCATAAGCGA 
GCTCAAAGGT TAAAGTTTCT ATGTGAAAGA AATGATAAGG TATTTTTTGC ATCCGTGCGA 
TCTGGAGGAA GTAGCCAAGT GTTTTTCATG ACCCTCAACA GAAATTCCAT GATGAACTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_002684922.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002684968.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X1, mRNA.

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CloneID	OBa02612
Clone ID Related Accession (Same CDS sequence)	XM_002684922.4 , XM_588438.8 , XM_002684922.5
Accession Version	XM_002684922.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002684922.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ACCGTCGGGA GAGAAGAAGC GACCTGGCCA CAGTGACAGC 
AATGGCTTTG CCGGCCACAT CAACCTCCCG GACCTGGTAC AGCAGAGCCA TTCCCCTGCG 
GGGACCCCCA CCGAGGGCCT GGGGCGCGTC TCCACCCACC CCCAGGAAAT GGACGCCGGG 
ACTGAATATG GCCTGGGGAG CAGCACCAAA GCCTCCTTCA CCCCCTTTGT GGACCCCAGG 
GTATACCAGA CATCTCCCAC TGATGAAGAT GAAGAGGATG AGGAATCATC AGCTGCGGCT 
CTGTTTACTA GCGAACTTCT TAGGCAAGAA CAGGCCAAAC TAAATGAAGC AAGAAAGATT 
TCAGTGGTAA ATGTAAACCC AACCAACATT CGGCCTCATA GCGACACACC GGAGATCAGA 
AAATATAAGA AGCGATTCAA TTCAGAAATA CTTTGTGCAG CTCTGTGGGG TGTAAACCTC 
CTGGTGGGAA CCGAAAATGG CCTGATGCTT TTGGACCGAA GTGGGCAAGG CAAAGTTTAT 
AATCTGATCA ACCGGAGGCG ATTTCAGCAG ATGGATGTGC TCGAGGGACT GAATGTCCTC 
GTGACAATAT CAGGAAAGAA GAATAAGTTA CGAGTTTACT ATCTTTCATG GTTAAGAAAC 
AGAATACTAC ACAATGACCC AGAAGTAGAA AAGAAACAAG GCTGGATCAC TGTGGGAGAC 
TTGGAAGGCT GTATACATTA CAAAGTTGTT AAATATGAAA GGATCAAATT CTTGGTGATT 
GCCTTAAAGA ATGCTGTGGA GATATATGCT TGGGCTCCTA AACCGTATCA TAAGTTCATG 
GCATTTAAGT CTTTTGCAGA TCTCCAGCAC AAGCCACTGC TTGTTGATCT CACAGTAGAA 
GAAGGTCAAA GATTAAAGGT TATTTTTGGT TCACACACTG GTTTCCATGT AATTGATGTT 
GATTCAGGAA ACTCTTATGA TATCTACATA CCATCTCATA TTCAGGGCAA TATCACTCCT 
CATGCCATTG TCATCTTGCC TAAGACAGAT GGAATGGAAA TGCTTGTTTG CTATGAGGAT 
GAGGGGGTGT ATGTAAACAC CTACGGCCGG ATAACTAAGG ATGTGGTGCT CCAATGGGGA 
GAAATGCCCA CGTCTGTGGC CTACATTCAT TCCAATCAGA TAATGGGCTG GGGCGAGAAA 
GCTATTGAGA TCCGGTCAGT GGAAACAGGA CATTTGGATG GAGTATTTAT GCATAAGCGA 
GCTCAAAGGT TAAAGTTTCT ATGTGAAAGA AATGATAAGG TATTTTTTGC ATCCGTGCGA 
TCTGGAGGAA GTAGCCAAGT GTTTTTCATG ACCCTCAACA GAAATTCCAT GATGAACTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002684968.1
CDS	365..4447
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_002684922.5
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002684922.5

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ACCGTCGGGA GAGAAGAAGC GACCTGGCCA CAGTGACAGC 
AATGGCTTTG CCGGCCACAT CAACCTCCCG GACCTGGTAC AGCAGAGCCA TTCCCCTGCG 
GGGACCCCCA CCGAGGGCCT GGGGCGCGTC TCCACCCACC CCCAGGAAAT GGACGCCGGG 
ACTGAATATG GCCTGGGGAG CAGCACCAAA GCCTCCTTCA CCCCCTTTGT GGACCCCAGG 
GTATACCAGA CATCTCCCAC TGATGAAGAT GAAGAGGATG AGGAATCATC AGCTGCGGCT 
CTGTTTACTA GCGAACTTCT TAGGCAAGAA CAGGCCAAAC TAAATGAAGC AAGAAAGATT 
TCAGTGGTAA ATGTAAACCC AACCAACATT CGGCCTCATA GCGACACACC GGAGATCAGA 
AAATATAAGA AGCGATTCAA TTCAGAAATA CTTTGTGCAG CTCTGTGGGG TGTAAACCTC 
CTGGTGGGAA CCGAAAATGG CCTGATGCTT TTGGACCGAA GTGGGCAAGG CAAAGTTTAT 
AATCTGATCA ACCGGAGGCG ATTTCAGCAG ATGGATGTGC TCGAGGGACT GAATGTCCTC 
GTGACAATAT CAGGAAAGAA GAATAAGTTA CGAGTTTACT ATCTTTCATG GTTAAGAAAC 
AGAATACTAC ACAATGACCC AGAAGTAGAA AAGAAACAAG GCTGGATCAC TGTGGGAGAC 
TTGGAAGGCT GTATACATTA CAAAGTTGTT AAATATGAAA GGATCAAATT CTTGGTGATT 
GCCTTAAAGA ATGCTGTGGA GATATATGCT TGGGCTCCTA AACCGTATCA TAAGTTCATG 
GCATTTAAGT CTTTTGCAGA TCTCCAGCAC AAGCCACTGC TTGTTGATCT CACAGTAGAA 
GAAGGTCAAA GATTAAAGGT TATTTTTGGT TCACACACTG GTTTCCATGT AATTGATGTT 
GATTCAGGAA ACTCTTATGA TATCTACATA CCATCTCATA TTCAGGGCAA TATCACTCCT 
CATGCCATTG TCATCTTGCC TAAGACAGAT GGAATGGAAA TGCTTGTTTG CTATGAGGAT 
GAGGGGGTGT ATGTAAACAC CTACGGCCGG ATAACTAAGG ATGTGGTGCT CCAATGGGGA 
GAAATGCCCA CGTCTGTGGC CTACATTCAT TCCAATCAGA TAATGGGCTG GGGCGAGAAA 
GCTATTGAGA TCCGGTCAGT GGAAACAGGA CATTTGGATG GAGTATTTAT GCATAAGCGA 
GCTCAAAGGT TAAAGTTTCT ATGTGAAAGA AATGATAAGG TATTTTTTGC ATCCGTGCGA 
TCTGGAGGAA GTAGCCAAGT GTTTTTCATG ACCCTCAACA GAAATTCCAT GATGAACTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_003585674.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003585722.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X2, mRNA.

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CloneID	OBa02613
Clone ID Related Accession (Same CDS sequence)	XM_003585674.4 , XM_003585674.3 , XM_003581731.3
Accession Version	XM_003585674.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003585674.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTGATCTGAC GGCATTGGCC 
AAAGAATTAA GAGAACTTCG GATTGAAGAA ACAAACCGCC CACTGAAGAA GGTAACAGAT 
TACTCCTCCT CCAGTGAGGA GTCAGAAAGC AGTGATGAAG AGGAGGAAGA TGGAGAGAGT 
GAGACCCAGG ATGGGACGGT GGCTGTCAGC GACATACCCA GACTGATACC CACAGGAGCC 
CCCGGGAGCG CTGAACAGTA CAATGTGGGA ATGGTCGGCA CACACGCGCT GGAGACCTCT 
CATGCAGATA CTTTCGGCAG CAGTATTTCA AGAGAAGGAA CCTTGATGAT AAGAGAACCG 
TCGGGAGAGA AGAAGCGACC TGGCCACAGT GACAGCAATG GCTTTGCCGG CCACATCAAC 
CTCCCGGACC TGGTACAGCA GAGCCATTCC CCTGCGGGGA CCCCCACCGA GGGCCTGGGG 
CGCGTCTCCA CCCACCCCCA GGAAATGGAC GCCGGGACTG AATATGGCCT GGGGAGCAGC 
ACCAAAGCCT CCTTCACCCC CTTTGTGGAC CCCAGGGTAT ACCAGACATC TCCCACTGAT 
GAAGATGAAG AGGATGAGGA ATCATCAGCT GCGGCTCTGT TTACTAGCGA ACTTCTTAGG 
CAAGAACAGG CCAAACTAAA TGAAGCAAGA AAGATTTCAG TGGTAAATGT AAACCCAACC 
AACATTCGGC CTCATAGCGA CACACCGGAG ATCAGAAAAT ATAAGAAGCG ATTCAATTCA 
GAAATACTTT GTGCAGCTCT GTGGGGTGTA AACCTCCTGG TGGGAACCGA AAATGGCCTG 
ATGCTTTTGG ACCGAAGTGG GCAAGGCAAA GTTTATAATC TGATCAACCG GAGGCGATTT 
CAGCAGATGG ATGTGCTCGA GGGACTGAAT GTCCTCGTGA CAATATCAGG AAAGAAGAAT 
AAGTTACGAG TTTACTATCT TTCATGGTTA AGAAACAGAA TACTACACAA TGACCCAGAA 
GTAGAAAAGA AACAAGGCTG GATCACTGTG GGAGACTTGG AAGGCTGTAT ACATTACAAA 
GTTGTTAAAT ATGAAAGGAT CAAATTCTTG GTGATTGCCT TAAAGAATGC TGTGGAGATA 
TATGCTTGGG CTCCTAAACC GTATCATAAG TTCATGGCAT TTAAGTCTTT TGCAGATCTC 
CAGCACAAGC CACTGCTTGT TGATCTCACA GTAGAAGAAG GTCAAAGATT AAAGGTTATT 
TTTGGTTCAC ACACTGGTTT CCATGTAATT GATGTTGATT CAGGAAACTC TTATGATATC 
TACATACCAT CTCATATTCA GGGCAATATC ACTCCTCATG CCATTGTCAT CTTGCCTAAG 
ACAGATGGAA TGGAAATGCT TGTTTGCTAT GAGGATGAGG GGGTGTATGT AAACACCTAC 
GGCCGGATAA CTAAGGATGT GGTGCTCCAA TGGGGAGAAA TGCCCACGTC TGTGGCCTAC 
ATTCATTCCA ATCAGATAAT GGGCTGGGGC GAGAAAGCTA TTGAGATCCG GTCAGTGGAA 
ACAGGACATT TGGATGGAGT ATTTATGCAT AAGCGAGCTC AAAGGTTAAA GTTTCTATGT 
GAAAGAAATG ATAAGGTATT TTTTGCATCC GTGCGATCTG GAGGAAGTAG CCAAGTGTTT 
TTCATGACCC TCAACAGAAA TTCCATGATG AACTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003585722.1
CDS	365..4423
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPADLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_003585674.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003585674.4

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTGATCTGAC GGCATTGGCC 
AAAGAATTAA GAGAACTTCG GATTGAAGAA ACAAACCGCC CACTGAAGAA GGTAACAGAT 
TACTCCTCCT CCAGTGAGGA GTCAGAAAGC AGTGATGAAG AGGAGGAAGA TGGAGAGAGT 
GAGACCCAGG ATGGGACGGT GGCTGTCAGC GACATACCCA GACTGATACC CACAGGAGCC 
CCCGGGAGCG CTGAACAGTA CAATGTGGGA ATGGTCGGCA CACACGCGCT GGAGACCTCT 
CATGCAGATA CTTTCGGCAG CAGTATTTCA AGAGAAGGAA CCTTGATGAT AAGAGAACCG 
TCGGGAGAGA AGAAGCGACC TGGCCACAGT GACAGCAATG GCTTTGCCGG CCACATCAAC 
CTCCCGGACC TGGTACAGCA GAGCCATTCC CCTGCGGGGA CCCCCACCGA GGGCCTGGGG 
CGCGTCTCCA CCCACCCCCA GGAAATGGAC GCCGGGACTG AATATGGCCT GGGGAGCAGC 
ACCAAAGCCT CCTTCACCCC CTTTGTGGAC CCCAGGGTAT ACCAGACATC TCCCACTGAT 
GAAGATGAAG AGGATGAGGA ATCATCAGCT GCGGCTCTGT TTACTAGCGA ACTTCTTAGG 
CAAGAACAGG CCAAACTAAA TGAAGCAAGA AAGATTTCAG TGGTAAATGT AAACCCAACC 
AACATTCGGC CTCATAGCGA CACACCGGAG ATCAGAAAAT ATAAGAAGCG ATTCAATTCA 
GAAATACTTT GTGCAGCTCT GTGGGGTGTA AACCTCCTGG TGGGAACCGA AAATGGCCTG 
ATGCTTTTGG ACCGAAGTGG GCAAGGCAAA GTTTATAATC TGATCAACCG GAGGCGATTT 
CAGCAGATGG ATGTGCTCGA GGGACTGAAT GTCCTCGTGA CAATATCAGG AAAGAAGAAT 
AAGTTACGAG TTTACTATCT TTCATGGTTA AGAAACAGAA TACTACACAA TGACCCAGAA 
GTAGAAAAGA AACAAGGCTG GATCACTGTG GGAGACTTGG AAGGCTGTAT ACATTACAAA 
GTTGTTAAAT ATGAAAGGAT CAAATTCTTG GTGATTGCCT TAAAGAATGC TGTGGAGATA 
TATGCTTGGG CTCCTAAACC GTATCATAAG TTCATGGCAT TTAAGTCTTT TGCAGATCTC 
CAGCACAAGC CACTGCTTGT TGATCTCACA GTAGAAGAAG GTCAAAGATT AAAGGTTATT 
TTTGGTTCAC ACACTGGTTT CCATGTAATT GATGTTGATT CAGGAAACTC TTATGATATC 
TACATACCAT CTCATATTCA GGGCAATATC ACTCCTCATG CCATTGTCAT CTTGCCTAAG 
ACAGATGGAA TGGAAATGCT TGTTTGCTAT GAGGATGAGG GGGTGTATGT AAACACCTAC 
GGCCGGATAA CTAAGGATGT GGTGCTCCAA TGGGGAGAAA TGCCCACGTC TGTGGCCTAC 
ATTCATTCCA ATCAGATAAT GGGCTGGGGC GAGAAAGCTA TTGAGATCCG GTCAGTGGAA 
ACAGGACATT TGGATGGAGT ATTTATGCAT AAGCGAGCTC AAAGGTTAAA GTTTCTATGT 
GAAAGAAATG ATAAGGTATT TTTTGCATCC GTGCGATCTG GAGGAAGTAG CCAAGTGTTT 
TTCATGACCC TCAACAGAAA TTCCATGATG AACTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_003585674.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003585722.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X6, mRNA.

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CloneID	OBa02613
Clone ID Related Accession (Same CDS sequence)	XM_003585674.4 , XM_003585674.3 , XM_003581731.3
Accession Version	XM_003585674.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_003585674.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTGATCTGAC GGCATTGGCC 
AAAGAATTAA GAGAACTTCG GATTGAAGAA ACAAACCGCC CACTGAAGAA GGTAACAGAT 
TACTCCTCCT CCAGTGAGGA GTCAGAAAGC AGTGATGAAG AGGAGGAAGA TGGAGAGAGT 
GAGACCCAGG ATGGGACGGT GGCTGTCAGC GACATACCCA GACTGATACC CACAGGAGCC 
CCCGGGAGCG CTGAACAGTA CAATGTGGGA ATGGTCGGCA CACACGCGCT GGAGACCTCT 
CATGCAGATA CTTTCGGCAG CAGTATTTCA AGAGAAGGAA CCTTGATGAT AAGAGAACCG 
TCGGGAGAGA AGAAGCGACC TGGCCACAGT GACAGCAATG GCTTTGCCGG CCACATCAAC 
CTCCCGGACC TGGTACAGCA GAGCCATTCC CCTGCGGGGA CCCCCACCGA GGGCCTGGGG 
CGCGTCTCCA CCCACCCCCA GGAAATGGAC GCCGGGACTG AATATGGCCT GGGGAGCAGC 
ACCAAAGCCT CCTTCACCCC CTTTGTGGAC CCCAGGGTAT ACCAGACATC TCCCACTGAT 
GAAGATGAAG AGGATGAGGA ATCATCAGCT GCGGCTCTGT TTACTAGCGA ACTTCTTAGG 
CAAGAACAGG CCAAACTAAA TGAAGCAAGA AAGATTTCAG TGGTAAATGT AAACCCAACC 
AACATTCGGC CTCATAGCGA CACACCGGAG ATCAGAAAAT ATAAGAAGCG ATTCAATTCA 
GAAATACTTT GTGCAGCTCT GTGGGGTGTA AACCTCCTGG TGGGAACCGA AAATGGCCTG 
ATGCTTTTGG ACCGAAGTGG GCAAGGCAAA GTTTATAATC TGATCAACCG GAGGCGATTT 
CAGCAGATGG ATGTGCTCGA GGGACTGAAT GTCCTCGTGA CAATATCAGG AAAGAAGAAT 
AAGTTACGAG TTTACTATCT TTCATGGTTA AGAAACAGAA TACTACACAA TGACCCAGAA 
GTAGAAAAGA AACAAGGCTG GATCACTGTG GGAGACTTGG AAGGCTGTAT ACATTACAAA 
GTTGTTAAAT ATGAAAGGAT CAAATTCTTG GTGATTGCCT TAAAGAATGC TGTGGAGATA 
TATGCTTGGG CTCCTAAACC GTATCATAAG TTCATGGCAT TTAAGTCTTT TGCAGATCTC 
CAGCACAAGC CACTGCTTGT TGATCTCACA GTAGAAGAAG GTCAAAGATT AAAGGTTATT 
TTTGGTTCAC ACACTGGTTT CCATGTAATT GATGTTGATT CAGGAAACTC TTATGATATC 
TACATACCAT CTCATATTCA GGGCAATATC ACTCCTCATG CCATTGTCAT CTTGCCTAAG 
ACAGATGGAA TGGAAATGCT TGTTTGCTAT GAGGATGAGG GGGTGTATGT AAACACCTAC 
GGCCGGATAA CTAAGGATGT GGTGCTCCAA TGGGGAGAAA TGCCCACGTC TGTGGCCTAC 
ATTCATTCCA ATCAGATAAT GGGCTGGGGC GAGAAAGCTA TTGAGATCCG GTCAGTGGAA 
ACAGGACATT TGGATGGAGT ATTTATGCAT AAGCGAGCTC AAAGGTTAAA GTTTCTATGT 
GAAAGAAATG ATAAGGTATT TTTTGCATCC GTGCGATCTG GAGGAAGTAG CCAAGTGTTT 
TTCATGACCC TCAACAGAAA TTCCATGATG AACTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003585722.1
CDS	341..4399
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPADLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_003585674.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003585674.3

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTGATCTGAC GGCATTGGCC 
AAAGAATTAA GAGAACTTCG GATTGAAGAA ACAAACCGCC CACTGAAGAA GGTAACAGAT 
TACTCCTCCT CCAGTGAGGA GTCAGAAAGC AGTGATGAAG AGGAGGAAGA TGGAGAGAGT 
GAGACCCAGG ATGGGACGGT GGCTGTCAGC GACATACCCA GACTGATACC CACAGGAGCC 
CCCGGGAGCG CTGAACAGTA CAATGTGGGA ATGGTCGGCA CACACGCGCT GGAGACCTCT 
CATGCAGATA CTTTCGGCAG CAGTATTTCA AGAGAAGGAA CCTTGATGAT AAGAGAACCG 
TCGGGAGAGA AGAAGCGACC TGGCCACAGT GACAGCAATG GCTTTGCCGG CCACATCAAC 
CTCCCGGACC TGGTACAGCA GAGCCATTCC CCTGCGGGGA CCCCCACCGA GGGCCTGGGG 
CGCGTCTCCA CCCACCCCCA GGAAATGGAC GCCGGGACTG AATATGGCCT GGGGAGCAGC 
ACCAAAGCCT CCTTCACCCC CTTTGTGGAC CCCAGGGTAT ACCAGACATC TCCCACTGAT 
GAAGATGAAG AGGATGAGGA ATCATCAGCT GCGGCTCTGT TTACTAGCGA ACTTCTTAGG 
CAAGAACAGG CCAAACTAAA TGAAGCAAGA AAGATTTCAG TGGTAAATGT AAACCCAACC 
AACATTCGGC CTCATAGCGA CACACCGGAG ATCAGAAAAT ATAAGAAGCG ATTCAATTCA 
GAAATACTTT GTGCAGCTCT GTGGGGTGTA AACCTCCTGG TGGGAACCGA AAATGGCCTG 
ATGCTTTTGG ACCGAAGTGG GCAAGGCAAA GTTTATAATC TGATCAACCG GAGGCGATTT 
CAGCAGATGG ATGTGCTCGA GGGACTGAAT GTCCTCGTGA CAATATCAGG AAAGAAGAAT 
AAGTTACGAG TTTACTATCT TTCATGGTTA AGAAACAGAA TACTACACAA TGACCCAGAA 
GTAGAAAAGA AACAAGGCTG GATCACTGTG GGAGACTTGG AAGGCTGTAT ACATTACAAA 
GTTGTTAAAT ATGAAAGGAT CAAATTCTTG GTGATTGCCT TAAAGAATGC TGTGGAGATA 
TATGCTTGGG CTCCTAAACC GTATCATAAG TTCATGGCAT TTAAGTCTTT TGCAGATCTC 
CAGCACAAGC CACTGCTTGT TGATCTCACA GTAGAAGAAG GTCAAAGATT AAAGGTTATT 
TTTGGTTCAC ACACTGGTTT CCATGTAATT GATGTTGATT CAGGAAACTC TTATGATATC 
TACATACCAT CTCATATTCA GGGCAATATC ACTCCTCATG CCATTGTCAT CTTGCCTAAG 
ACAGATGGAA TGGAAATGCT TGTTTGCTAT GAGGATGAGG GGGTGTATGT AAACACCTAC 
GGCCGGATAA CTAAGGATGT GGTGCTCCAA TGGGGAGAAA TGCCCACGTC TGTGGCCTAC 
ATTCATTCCA ATCAGATAAT GGGCTGGGGC GAGAAAGCTA TTGAGATCCG GTCAGTGGAA 
ACAGGACATT TGGATGGAGT ATTTATGCAT AAGCGAGCTC AAAGGTTAAA GTTTCTATGT 
GAAAGAAATG ATAAGGTATT TTTTGCATCC GTGCGATCTG GAGGAAGTAG CCAAGTGTTT 
TTCATGACCC TCAACAGAAA TTCCATGATG AACTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_003581731.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003581779.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa02613
Clone ID Related Accession (Same CDS sequence)	XM_003585674.4 , XM_003585674.3 , XM_003581731.3
Accession Version	XM_003581731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644547.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_003581731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTGATCTGAC GGCATTGGCC 
AAAGAATTAA GAGAACTTCG GATTGAAGAA ACAAACCGCC CACTGAAGAA GGTAACAGAT 
TACTCCTCCT CCAGTGAGGA GTCAGAAAGC AGTGATGAAG AGGAGGAAGA TGGAGAGAGT 
GAGACCCAGG ATGGGACGGT GGCTGTCAGC GACATACCCA GACTGATACC CACAGGAGCC 
CCCGGGAGCG CTGAACAGTA CAATGTGGGA ATGGTCGGCA CACACGCGCT GGAGACCTCT 
CATGCAGATA CTTTCGGCAG CAGTATTTCA AGAGAAGGAA CCTTGATGAT AAGAGAACCG 
TCGGGAGAGA AGAAGCGACC TGGCCACAGT GACAGCAATG GCTTTGCCGG CCACATCAAC 
CTCCCGGACC TGGTACAGCA GAGCCATTCC CCTGCGGGGA CCCCCACCGA GGGCCTGGGG 
CGCGTCTCCA CCCACCCCCA GGAAATGGAC GCCGGGACTG AATATGGCCT GGGGAGCAGC 
ACCAAAGCCT CCTTCACCCC CTTTGTGGAC CCCAGGGTAT ACCAGACATC TCCCACTGAT 
GAAGATGAAG AGGATGAGGA ATCATCAGCT GCGGCTCTGT TTACTAGCGA ACTTCTTAGG 
CAAGAACAGG CCAAACTAAA TGAAGCAAGA AAGATTTCAG TGGTAAATGT AAACCCAACC 
AACATTCGGC CTCATAGCGA CACACCGGAG ATCAGAAAAT ATAAGAAGCG ATTCAATTCA 
GAAATACTTT GTGCAGCTCT GTGGGGTGTA AACCTCCTGG TGGGAACCGA AAATGGCCTG 
ATGCTTTTGG ACCGAAGTGG GCAAGGCAAA GTTTATAATC TGATCAACCG GAGGCGATTT 
CAGCAGATGG ATGTGCTCGA GGGACTGAAT GTCCTCGTGA CAATATCAGG AAAGAAGAAT 
AAGTTACGAG TTTACTATCT TTCATGGTTA AGAAACAGAA TACTACACAA TGACCCAGAA 
GTAGAAAAGA AACAAGGCTG GATCACTGTG GGAGACTTGG AAGGCTGTAT ACATTACAAA 
GTTGTTAAAT ATGAAAGGAT CAAATTCTTG GTGATTGCCT TAAAGAATGC TGTGGAGATA 
TATGCTTGGG CTCCTAAACC GTATCATAAG TTCATGGCAT TTAAGTCTTT TGCAGATCTC 
CAGCACAAGC CACTGCTTGT TGATCTCACA GTAGAAGAAG GTCAAAGATT AAAGGTTATT 
TTTGGTTCAC ACACTGGTTT CCATGTAATT GATGTTGATT CAGGAAACTC TTATGATATC 
TACATACCAT CTCATATTCA GGGCAATATC ACTCCTCATG CCATTGTCAT CTTGCCTAAG 
ACAGATGGAA TGGAAATGCT TGTTTGCTAT GAGGATGAGG GGGTGTATGT AAACACCTAC 
GGCCGGATAA CTAAGGATGT GGTGCTCCAA TGGGGAGAAA TGCCCACGTC TGTGGCCTAC 
ATTCATTCCA ATCAGATAAT GGGCTGGGGC GAGAAAGCTA TTGAGATCCG GTCAGTGGAA 
ACAGGACATT TGGATGGAGT ATTTATGCAT AAGCGAGCTC AAAGGTTAAA GTTTCTATGT 
GAAAGAAATG ATAAGGTATT TTTTGCATCC GTGCGATCTG GAGGAAGTAG CCAAGTGTTT 
TTCATGACCC TCAACAGAAA TTCCATGATG AACTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003581779.1
CDS	341..4399
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPADLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_003581731.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003581731.3

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTGATCTGAC GGCATTGGCC 
AAAGAATTAA GAGAACTTCG GATTGAAGAA ACAAACCGCC CACTGAAGAA GGTAACAGAT 
TACTCCTCCT CCAGTGAGGA GTCAGAAAGC AGTGATGAAG AGGAGGAAGA TGGAGAGAGT 
GAGACCCAGG ATGGGACGGT GGCTGTCAGC GACATACCCA GACTGATACC CACAGGAGCC 
CCCGGGAGCG CTGAACAGTA CAATGTGGGA ATGGTCGGCA CACACGCGCT GGAGACCTCT 
CATGCAGATA CTTTCGGCAG CAGTATTTCA AGAGAAGGAA CCTTGATGAT AAGAGAACCG 
TCGGGAGAGA AGAAGCGACC TGGCCACAGT GACAGCAATG GCTTTGCCGG CCACATCAAC 
CTCCCGGACC TGGTACAGCA GAGCCATTCC CCTGCGGGGA CCCCCACCGA GGGCCTGGGG 
CGCGTCTCCA CCCACCCCCA GGAAATGGAC GCCGGGACTG AATATGGCCT GGGGAGCAGC 
ACCAAAGCCT CCTTCACCCC CTTTGTGGAC CCCAGGGTAT ACCAGACATC TCCCACTGAT 
GAAGATGAAG AGGATGAGGA ATCATCAGCT GCGGCTCTGT TTACTAGCGA ACTTCTTAGG 
CAAGAACAGG CCAAACTAAA TGAAGCAAGA AAGATTTCAG TGGTAAATGT AAACCCAACC 
AACATTCGGC CTCATAGCGA CACACCGGAG ATCAGAAAAT ATAAGAAGCG ATTCAATTCA 
GAAATACTTT GTGCAGCTCT GTGGGGTGTA AACCTCCTGG TGGGAACCGA AAATGGCCTG 
ATGCTTTTGG ACCGAAGTGG GCAAGGCAAA GTTTATAATC TGATCAACCG GAGGCGATTT 
CAGCAGATGG ATGTGCTCGA GGGACTGAAT GTCCTCGTGA CAATATCAGG AAAGAAGAAT 
AAGTTACGAG TTTACTATCT TTCATGGTTA AGAAACAGAA TACTACACAA TGACCCAGAA 
GTAGAAAAGA AACAAGGCTG GATCACTGTG GGAGACTTGG AAGGCTGTAT ACATTACAAA 
GTTGTTAAAT ATGAAAGGAT CAAATTCTTG GTGATTGCCT TAAAGAATGC TGTGGAGATA 
TATGCTTGGG CTCCTAAACC GTATCATAAG TTCATGGCAT TTAAGTCTTT TGCAGATCTC 
CAGCACAAGC CACTGCTTGT TGATCTCACA GTAGAAGAAG GTCAAAGATT AAAGGTTATT 
TTTGGTTCAC ACACTGGTTT CCATGTAATT GATGTTGATT CAGGAAACTC TTATGATATC 
TACATACCAT CTCATATTCA GGGCAATATC ACTCCTCATG CCATTGTCAT CTTGCCTAAG 
ACAGATGGAA TGGAAATGCT TGTTTGCTAT GAGGATGAGG GGGTGTATGT AAACACCTAC 
GGCCGGATAA CTAAGGATGT GGTGCTCCAA TGGGGAGAAA TGCCCACGTC TGTGGCCTAC 
ATTCATTCCA ATCAGATAAT GGGCTGGGGC GAGAAAGCTA TTGAGATCCG GTCAGTGGAA 
ACAGGACATT TGGATGGAGT ATTTATGCAT AAGCGAGCTC AAAGGTTAAA GTTTCTATGT 
GAAAGAAATG ATAAGGTATT TTTTGCATCC GTGCGATCTG GAGGAAGTAG CCAAGTGTTT 
TTCATGACCC TCAACAGAAA TTCCATGATG AACTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_010824381.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822683.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X2, mRNA.

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CloneID	OBa54384
Clone ID Related Accession (Same CDS sequence)	XM_010824381.1 , XM_010801392.1
Accession Version	XM_010824381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644547.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTGATCTGAC GGCATTGGCC 
AAAGAATTAA GAGAACTTCG GATTGAAGAA ACAAACCGCC CACTGAAGAA GGTAACAGAT 
TACTCCTCCT CCAGTGAGGA GTCAGAAAGC AGTGATGAAG AGGAGGAAGA TGGAGAGAGT 
GAGACCCAGG ATGGGACGGT GGCTGTCAGC GACATACCCA GACTGATACC CACAGGAGCC 
CCCGGGAGCG CTGAACAGTA CAATGTGGGA ATGGTCGGCA CACACGCGCT GGAGACCTCT 
CATGCAGATA CTTTCGGCAG CAGTATTTCA AGAGAAGGAA CCTTGATGAT AAGAGAACCG 
TCGGGAGAGA AGAAGCGACC TGGCCACAGT GACAGCAATG GCTTTGCCGG CCACATCAAC 
CTCCCGGACC TGGTACAGCA GAGCCATTCC CCTGCGGGGA CCCCCACCGA GGGCCTGGGG 
CGCGTCTCCA CCCACCCCCA GGAAATGGAC GCCGGGACTG AATATGGCCT GGGGAGCAGC 
ACCAAAGCCT CCTTCACCCC CTTTGTGGAC CCCAGGGTAT ACCAGACATC TCCCACTGAT 
GAAGATGAAG AGGATGAGGA ATCATCAGCT GCGGGAACCC AGAGGTATCC AGGGGCCCTG 
GGAAAAGCTG TTCCTCCGAG ATCTCCAAAC TCACCACCTA TTTCTCAAAA GCAGTTTCTG 
GCTCTGTTTA CTAGCGAACT TCTTAGGCAA GAACAGGCCA AACTAAATGA AGCAAGAAAG 
ATTTCAGTGG TAAATGTAAA CCCAACCAAC ATTCGGCCTC ATAGCGACAC ACCGGAGATC 
AGAAAATATA AGAAGCGATT CAATTCAGAA ATACTTTGTG CAGCTCTGTG GGGTGTAAAC 
CTCCTGGTGG GAACCGAAAA TGGCCTGATG CTTTTGGACC GAAGTGGGCA AGGCAAAGTT 
TATAATCTGA TCAACCGGAG GCGATTTCAG CAGATGGATG TGCTCGAGGG ACTGAATGTC 
CTCGTGACAA TATCAGGAAA GAAGAATAAG TTACGAGTTT ACTATCTTTC ATGGTTAAGA 
AACAGAATAC TACACAATGA CCCAGAAGTA GAAAAGAAAC AAGGCTGGAT CACTGTGGGA 
GACTTGGAAG GCTGTATACA TTACAAAGTT GTTAAATATG AAAGGATCAA ATTCTTGGTG 
ATTGCCTTAA AGAATGCTGT GGAGATATAT GCTTGGGCTC CTAAACCGTA TCATAAGTTC 
ATGGCATTTA AGTCTTTTGC AGATCTCCAG CACAAGCCAC TGCTTGTTGA TCTCACAGTA 
GAAGAAGGTC AAAGATTAAA GGTTATTTTT GGTTCACACA CTGGTTTCCA TGTAATTGAT 
GTTGATTCAG GAAACTCTTA TGATATCTAC ATACCATCTC ATATTCAGGG CAATATCACT 
CCTCATGCCA TTGTCATCTT GCCTAAGACA GATGGAATGG AAATGCTTGT TTGCTATGAG 
GATGAGGGGG TGTATGTAAA CACCTACGGC CGGATAACTA AGGATGTGGT GCTCCAATGG 
GGAGAAATGC CCACGTCTGT GGCCTACATT CATTCCAATC AGATAATGGG CTGGGGCGAG 
AAAGCTATTG AGATCCGGTC AGTGGAAACA GGACATTTGG ATGGAGTATT TATGCATAAG 
CGAGCTCAAA GGTTAAAGTT TCTATGTGAA AGAAATGATA AGGTATTTTT TGCATCCGTG 
CGATCTGGAG GAAGTAGCCA AGTGTTTTTC ATGACCCTCA ACAGAAATTC CATGATGAAC 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822683.1
CDS	341..4486
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPADLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAGTQRYPGALGKAVPPRSPNSPPISQKQFLALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_010824381.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824381.1

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTGATCTGAC GGCATTGGCC 
AAAGAATTAA GAGAACTTCG GATTGAAGAA ACAAACCGCC CACTGAAGAA GGTAACAGAT 
TACTCCTCCT CCAGTGAGGA GTCAGAAAGC AGTGATGAAG AGGAGGAAGA TGGAGAGAGT 
GAGACCCAGG ATGGGACGGT GGCTGTCAGC GACATACCCA GACTGATACC CACAGGAGCC 
CCCGGGAGCG CTGAACAGTA CAATGTGGGA ATGGTCGGCA CACACGCGCT GGAGACCTCT 
CATGCAGATA CTTTCGGCAG CAGTATTTCA AGAGAAGGAA CCTTGATGAT AAGAGAACCG 
TCGGGAGAGA AGAAGCGACC TGGCCACAGT GACAGCAATG GCTTTGCCGG CCACATCAAC 
CTCCCGGACC TGGTACAGCA GAGCCATTCC CCTGCGGGGA CCCCCACCGA GGGCCTGGGG 
CGCGTCTCCA CCCACCCCCA GGAAATGGAC GCCGGGACTG AATATGGCCT GGGGAGCAGC 
ACCAAAGCCT CCTTCACCCC CTTTGTGGAC CCCAGGGTAT ACCAGACATC TCCCACTGAT 
GAAGATGAAG AGGATGAGGA ATCATCAGCT GCGGGAACCC AGAGGTATCC AGGGGCCCTG 
GGAAAAGCTG TTCCTCCGAG ATCTCCAAAC TCACCACCTA TTTCTCAAAA GCAGTTTCTG 
GCTCTGTTTA CTAGCGAACT TCTTAGGCAA GAACAGGCCA AACTAAATGA AGCAAGAAAG 
ATTTCAGTGG TAAATGTAAA CCCAACCAAC ATTCGGCCTC ATAGCGACAC ACCGGAGATC 
AGAAAATATA AGAAGCGATT CAATTCAGAA ATACTTTGTG CAGCTCTGTG GGGTGTAAAC 
CTCCTGGTGG GAACCGAAAA TGGCCTGATG CTTTTGGACC GAAGTGGGCA AGGCAAAGTT 
TATAATCTGA TCAACCGGAG GCGATTTCAG CAGATGGATG TGCTCGAGGG ACTGAATGTC 
CTCGTGACAA TATCAGGAAA GAAGAATAAG TTACGAGTTT ACTATCTTTC ATGGTTAAGA 
AACAGAATAC TACACAATGA CCCAGAAGTA GAAAAGAAAC AAGGCTGGAT CACTGTGGGA 
GACTTGGAAG GCTGTATACA TTACAAAGTT GTTAAATATG AAAGGATCAA ATTCTTGGTG 
ATTGCCTTAA AGAATGCTGT GGAGATATAT GCTTGGGCTC CTAAACCGTA TCATAAGTTC 
ATGGCATTTA AGTCTTTTGC AGATCTCCAG CACAAGCCAC TGCTTGTTGA TCTCACAGTA 
GAAGAAGGTC AAAGATTAAA GGTTATTTTT GGTTCACACA CTGGTTTCCA TGTAATTGAT 
GTTGATTCAG GAAACTCTTA TGATATCTAC ATACCATCTC ATATTCAGGG CAATATCACT 
CCTCATGCCA TTGTCATCTT GCCTAAGACA GATGGAATGG AAATGCTTGT TTGCTATGAG 
GATGAGGGGG TGTATGTAAA CACCTACGGC CGGATAACTA AGGATGTGGT GCTCCAATGG 
GGAGAAATGC CCACGTCTGT GGCCTACATT CATTCCAATC AGATAATGGG CTGGGGCGAG 
AAAGCTATTG AGATCCGGTC AGTGGAAACA GGACATTTGG ATGGAGTATT TATGCATAAG 
CGAGCTCAAA GGTTAAAGTT TCTATGTGAA AGAAATGATA AGGTATTTTT TGCATCCGTG 
CGATCTGGAG GAAGTAGCCA AGTGTTTTTC ATGACCCTCA ACAGAAATTC CATGATGAAC 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_010801392.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799694.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa54384
Clone ID Related Accession (Same CDS sequence)	XM_010824381.1 , XM_010801392.1
Accession Version	XM_010801392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTGATCTGAC GGCATTGGCC 
AAAGAATTAA GAGAACTTCG GATTGAAGAA ACAAACCGCC CACTGAAGAA GGTAACAGAT 
TACTCCTCCT CCAGTGAGGA GTCAGAAAGC AGTGATGAAG AGGAGGAAGA TGGAGAGAGT 
GAGACCCAGG ATGGGACGGT GGCTGTCAGC GACATACCCA GACTGATACC CACAGGAGCC 
CCCGGGAGCG CTGAACAGTA CAATGTGGGA ATGGTCGGCA CACACGCGCT GGAGACCTCT 
CATGCAGATA CTTTCGGCAG CAGTATTTCA AGAGAAGGAA CCTTGATGAT AAGAGAACCG 
TCGGGAGAGA AGAAGCGACC TGGCCACAGT GACAGCAATG GCTTTGCCGG CCACATCAAC 
CTCCCGGACC TGGTACAGCA GAGCCATTCC CCTGCGGGGA CCCCCACCGA GGGCCTGGGG 
CGCGTCTCCA CCCACCCCCA GGAAATGGAC GCCGGGACTG AATATGGCCT GGGGAGCAGC 
ACCAAAGCCT CCTTCACCCC CTTTGTGGAC CCCAGGGTAT ACCAGACATC TCCCACTGAT 
GAAGATGAAG AGGATGAGGA ATCATCAGCT GCGGGAACCC AGAGGTATCC AGGGGCCCTG 
GGAAAAGCTG TTCCTCCGAG ATCTCCAAAC TCACCACCTA TTTCTCAAAA GCAGTTTCTG 
GCTCTGTTTA CTAGCGAACT TCTTAGGCAA GAACAGGCCA AACTAAATGA AGCAAGAAAG 
ATTTCAGTGG TAAATGTAAA CCCAACCAAC ATTCGGCCTC ATAGCGACAC ACCGGAGATC 
AGAAAATATA AGAAGCGATT CAATTCAGAA ATACTTTGTG CAGCTCTGTG GGGTGTAAAC 
CTCCTGGTGG GAACCGAAAA TGGCCTGATG CTTTTGGACC GAAGTGGGCA AGGCAAAGTT 
TATAATCTGA TCAACCGGAG GCGATTTCAG CAGATGGATG TGCTCGAGGG ACTGAATGTC 
CTCGTGACAA TATCAGGAAA GAAGAATAAG TTACGAGTTT ACTATCTTTC ATGGTTAAGA 
AACAGAATAC TACACAATGA CCCAGAAGTA GAAAAGAAAC AAGGCTGGAT CACTGTGGGA 
GACTTGGAAG GCTGTATACA TTACAAAGTT GTTAAATATG AAAGGATCAA ATTCTTGGTG 
ATTGCCTTAA AGAATGCTGT GGAGATATAT GCTTGGGCTC CTAAACCGTA TCATAAGTTC 
ATGGCATTTA AGTCTTTTGC AGATCTCCAG CACAAGCCAC TGCTTGTTGA TCTCACAGTA 
GAAGAAGGTC AAAGATTAAA GGTTATTTTT GGTTCACACA CTGGTTTCCA TGTAATTGAT 
GTTGATTCAG GAAACTCTTA TGATATCTAC ATACCATCTC ATATTCAGGG CAATATCACT 
CCTCATGCCA TTGTCATCTT GCCTAAGACA GATGGAATGG AAATGCTTGT TTGCTATGAG 
GATGAGGGGG TGTATGTAAA CACCTACGGC CGGATAACTA AGGATGTGGT GCTCCAATGG 
GGAGAAATGC CCACGTCTGT GGCCTACATT CATTCCAATC AGATAATGGG CTGGGGCGAG 
AAAGCTATTG AGATCCGGTC AGTGGAAACA GGACATTTGG ATGGAGTATT TATGCATAAG 
CGAGCTCAAA GGTTAAAGTT TCTATGTGAA AGAAATGATA AGGTATTTTT TGCATCCGTG 
CGATCTGGAG GAAGTAGCCA AGTGTTTTTC ATGACCCTCA ACAGAAATTC CATGATGAAC 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799694.1
CDS	341..4486
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPADLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAGTQRYPGALGKAVPPRSPNSPPISQKQFLALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_010801392.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801392.1

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTGATCTGAC GGCATTGGCC 
AAAGAATTAA GAGAACTTCG GATTGAAGAA ACAAACCGCC CACTGAAGAA GGTAACAGAT 
TACTCCTCCT CCAGTGAGGA GTCAGAAAGC AGTGATGAAG AGGAGGAAGA TGGAGAGAGT 
GAGACCCAGG ATGGGACGGT GGCTGTCAGC GACATACCCA GACTGATACC CACAGGAGCC 
CCCGGGAGCG CTGAACAGTA CAATGTGGGA ATGGTCGGCA CACACGCGCT GGAGACCTCT 
CATGCAGATA CTTTCGGCAG CAGTATTTCA AGAGAAGGAA CCTTGATGAT AAGAGAACCG 
TCGGGAGAGA AGAAGCGACC TGGCCACAGT GACAGCAATG GCTTTGCCGG CCACATCAAC 
CTCCCGGACC TGGTACAGCA GAGCCATTCC CCTGCGGGGA CCCCCACCGA GGGCCTGGGG 
CGCGTCTCCA CCCACCCCCA GGAAATGGAC GCCGGGACTG AATATGGCCT GGGGAGCAGC 
ACCAAAGCCT CCTTCACCCC CTTTGTGGAC CCCAGGGTAT ACCAGACATC TCCCACTGAT 
GAAGATGAAG AGGATGAGGA ATCATCAGCT GCGGGAACCC AGAGGTATCC AGGGGCCCTG 
GGAAAAGCTG TTCCTCCGAG ATCTCCAAAC TCACCACCTA TTTCTCAAAA GCAGTTTCTG 
GCTCTGTTTA CTAGCGAACT TCTTAGGCAA GAACAGGCCA AACTAAATGA AGCAAGAAAG 
ATTTCAGTGG TAAATGTAAA CCCAACCAAC ATTCGGCCTC ATAGCGACAC ACCGGAGATC 
AGAAAATATA AGAAGCGATT CAATTCAGAA ATACTTTGTG CAGCTCTGTG GGGTGTAAAC 
CTCCTGGTGG GAACCGAAAA TGGCCTGATG CTTTTGGACC GAAGTGGGCA AGGCAAAGTT 
TATAATCTGA TCAACCGGAG GCGATTTCAG CAGATGGATG TGCTCGAGGG ACTGAATGTC 
CTCGTGACAA TATCAGGAAA GAAGAATAAG TTACGAGTTT ACTATCTTTC ATGGTTAAGA 
AACAGAATAC TACACAATGA CCCAGAAGTA GAAAAGAAAC AAGGCTGGAT CACTGTGGGA 
GACTTGGAAG GCTGTATACA TTACAAAGTT GTTAAATATG AAAGGATCAA ATTCTTGGTG 
ATTGCCTTAA AGAATGCTGT GGAGATATAT GCTTGGGCTC CTAAACCGTA TCATAAGTTC 
ATGGCATTTA AGTCTTTTGC AGATCTCCAG CACAAGCCAC TGCTTGTTGA TCTCACAGTA 
GAAGAAGGTC AAAGATTAAA GGTTATTTTT GGTTCACACA CTGGTTTCCA TGTAATTGAT 
GTTGATTCAG GAAACTCTTA TGATATCTAC ATACCATCTC ATATTCAGGG CAATATCACT 
CCTCATGCCA TTGTCATCTT GCCTAAGACA GATGGAATGG AAATGCTTGT TTGCTATGAG 
GATGAGGGGG TGTATGTAAA CACCTACGGC CGGATAACTA AGGATGTGGT GCTCCAATGG 
GGAGAAATGC CCACGTCTGT GGCCTACATT CATTCCAATC AGATAATGGG CTGGGGCGAG 
AAAGCTATTG AGATCCGGTC AGTGGAAACA GGACATTTGG ATGGAGTATT TATGCATAAG 
CGAGCTCAAA GGTTAAAGTT TCTATGTGAA AGAAATGATA AGGTATTTTT TGCATCCGTG 
CGATCTGGAG GAAGTAGCCA AGTGTTTTTC ATGACCCTCA ACAGAAATTC CATGATGAAC 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_010801393.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799695.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa54385
Clone ID Related Accession (Same CDS sequence)	XM_010801393.1 , XM_010824382.1
Accession Version	XM_010801393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTAGCTA TAAGAAAGCT ATAGATGAGG ATCTGACGGC ATTGGCCAAA 
GAATTAAGAG AACTTCGGAT TGAAGAAACA AACCGCCCAC TGAAGAAGGT AACAGATTAC 
TCCTCCTCCA GTGAGGAGTC AGAAAGCAGT GATGAAGAGG AGGAAGATGG AGAGAGTGAG 
ACCCAGGATG GGACGGTGGC TGTCAGCGAC ATACCCAGAC TGATACCCAC AGGAGCCCCC 
GGGAGCGCTG AACAGTACAA TGTGGGAATG GTCGGCACAC ACGCGCTGGA GACCTCTCAT 
GCAGATACTT TCGGCAGCAG TATTTCAAGA GAAGGAACCT TGATGATAAG AGAACCGTCG 
GGAGAGAAGA AGCGACCTGG CCACAGTGAC AGCAATGGCT TTGCCGGCCA CATCAACCTC 
CCGGACCTGG TACAGCAGAG CCATTCCCCT GCGGGGACCC CCACCGAGGG CCTGGGGCGC 
GTCTCCACCC ACCCCCAGGA AATGGACGCC GGGACTGAAT ATGGCCTGGG GAGCAGCACC 
AAAGCCTCCT TCACCCCCTT TGTGGACCCC AGGGTATACC AGACATCTCC CACTGATGAA 
GATGAAGAGG ATGAGGAATC ATCAGCTGCG GGAACCCAGA GGTATCCAGG GGCCCTGGGA 
AAAGCTGTTC CTCCGAGATC TCCAAACTCA CCACCTATTT CTCAAAAGCA GTTTCTGGCT 
CTGTTTACTA GCGAACTTCT TAGGCAAGAA CAGGCCAAAC TAAATGAAGC AAGAAAGATT 
TCAGTGGTAA ATGTAAACCC AACCAACATT CGGCCTCATA GCGACACACC GGAGATCAGA 
AAATATAAGA AGCGATTCAA TTCAGAAATA CTTTGTGCAG CTCTGTGGGG TGTAAACCTC 
CTGGTGGGAA CCGAAAATGG CCTGATGCTT TTGGACCGAA GTGGGCAAGG CAAAGTTTAT 
AATCTGATCA ACCGGAGGCG ATTTCAGCAG ATGGATGTGC TCGAGGGACT GAATGTCCTC 
GTGACAATAT CAGGAAAGAA GAATAAGTTA CGAGTTTACT ATCTTTCATG GTTAAGAAAC 
AGAATACTAC ACAATGACCC AGAAGTAGAA AAGAAACAAG GCTGGATCAC TGTGGGAGAC 
TTGGAAGGCT GTATACATTA CAAAGTTGTT AAATATGAAA GGATCAAATT CTTGGTGATT 
GCCTTAAAGA ATGCTGTGGA GATATATGCT TGGGCTCCTA AACCGTATCA TAAGTTCATG 
GCATTTAAGT CTTTTGCAGA TCTCCAGCAC AAGCCACTGC TTGTTGATCT CACAGTAGAA 
GAAGGTCAAA GATTAAAGGT TATTTTTGGT TCACACACTG GTTTCCATGT AATTGATGTT 
GATTCAGGAA ACTCTTATGA TATCTACATA CCATCTCATA TTCAGGGCAA TATCACTCCT 
CATGCCATTG TCATCTTGCC TAAGACAGAT GGAATGGAAA TGCTTGTTTG CTATGAGGAT 
GAGGGGGTGT ATGTAAACAC CTACGGCCGG ATAACTAAGG ATGTGGTGCT CCAATGGGGA 
GAAATGCCCA CGTCTGTGGC CTACATTCAT TCCAATCAGA TAATGGGCTG GGGCGAGAAA 
GCTATTGAGA TCCGGTCAGT GGAAACAGGA CATTTGGATG GAGTATTTAT GCATAAGCGA 
GCTCAAAGGT TAAAGTTTCT ATGTGAAAGA AATGATAAGG TATTTTTTGC ATCCGTGCGA 
TCTGGAGGAA GTAGCCAAGT GTTTTTCATG ACCCTCAACA GAAATTCCAT GATGAACTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799695.1
CDS	341..4423
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAGTQRYPGALGKAVPPRSPNSPPISQKQFLALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_010801393.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801393.1

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTAGCTA TAAGAAAGCT ATAGATGAGG ATCTGACGGC ATTGGCCAAA 
GAATTAAGAG AACTTCGGAT TGAAGAAACA AACCGCCCAC TGAAGAAGGT AACAGATTAC 
TCCTCCTCCA GTGAGGAGTC AGAAAGCAGT GATGAAGAGG AGGAAGATGG AGAGAGTGAG 
ACCCAGGATG GGACGGTGGC TGTCAGCGAC ATACCCAGAC TGATACCCAC AGGAGCCCCC 
GGGAGCGCTG AACAGTACAA TGTGGGAATG GTCGGCACAC ACGCGCTGGA GACCTCTCAT 
GCAGATACTT TCGGCAGCAG TATTTCAAGA GAAGGAACCT TGATGATAAG AGAACCGTCG 
GGAGAGAAGA AGCGACCTGG CCACAGTGAC AGCAATGGCT TTGCCGGCCA CATCAACCTC 
CCGGACCTGG TACAGCAGAG CCATTCCCCT GCGGGGACCC CCACCGAGGG CCTGGGGCGC 
GTCTCCACCC ACCCCCAGGA AATGGACGCC GGGACTGAAT ATGGCCTGGG GAGCAGCACC 
AAAGCCTCCT TCACCCCCTT TGTGGACCCC AGGGTATACC AGACATCTCC CACTGATGAA 
GATGAAGAGG ATGAGGAATC ATCAGCTGCG GGAACCCAGA GGTATCCAGG GGCCCTGGGA 
AAAGCTGTTC CTCCGAGATC TCCAAACTCA CCACCTATTT CTCAAAAGCA GTTTCTGGCT 
CTGTTTACTA GCGAACTTCT TAGGCAAGAA CAGGCCAAAC TAAATGAAGC AAGAAAGATT 
TCAGTGGTAA ATGTAAACCC AACCAACATT CGGCCTCATA GCGACACACC GGAGATCAGA 
AAATATAAGA AGCGATTCAA TTCAGAAATA CTTTGTGCAG CTCTGTGGGG TGTAAACCTC 
CTGGTGGGAA CCGAAAATGG CCTGATGCTT TTGGACCGAA GTGGGCAAGG CAAAGTTTAT 
AATCTGATCA ACCGGAGGCG ATTTCAGCAG ATGGATGTGC TCGAGGGACT GAATGTCCTC 
GTGACAATAT CAGGAAAGAA GAATAAGTTA CGAGTTTACT ATCTTTCATG GTTAAGAAAC 
AGAATACTAC ACAATGACCC AGAAGTAGAA AAGAAACAAG GCTGGATCAC TGTGGGAGAC 
TTGGAAGGCT GTATACATTA CAAAGTTGTT AAATATGAAA GGATCAAATT CTTGGTGATT 
GCCTTAAAGA ATGCTGTGGA GATATATGCT TGGGCTCCTA AACCGTATCA TAAGTTCATG 
GCATTTAAGT CTTTTGCAGA TCTCCAGCAC AAGCCACTGC TTGTTGATCT CACAGTAGAA 
GAAGGTCAAA GATTAAAGGT TATTTTTGGT TCACACACTG GTTTCCATGT AATTGATGTT 
GATTCAGGAA ACTCTTATGA TATCTACATA CCATCTCATA TTCAGGGCAA TATCACTCCT 
CATGCCATTG TCATCTTGCC TAAGACAGAT GGAATGGAAA TGCTTGTTTG CTATGAGGAT 
GAGGGGGTGT ATGTAAACAC CTACGGCCGG ATAACTAAGG ATGTGGTGCT CCAATGGGGA 
GAAATGCCCA CGTCTGTGGC CTACATTCAT TCCAATCAGA TAATGGGCTG GGGCGAGAAA 
GCTATTGAGA TCCGGTCAGT GGAAACAGGA CATTTGGATG GAGTATTTAT GCATAAGCGA 
GCTCAAAGGT TAAAGTTTCT ATGTGAAAGA AATGATAAGG TATTTTTTGC ATCCGTGCGA 
TCTGGAGGAA GTAGCCAAGT GTTTTTCATG ACCCTCAACA GAAATTCCAT GATGAACTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_010824382.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822684.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X4, mRNA.

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CloneID	OBa54385
Clone ID Related Accession (Same CDS sequence)	XM_010801393.1 , XM_010824382.1
Accession Version	XM_010824382.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644547.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTAGCTA TAAGAAAGCT ATAGATGAGG ATCTGACGGC ATTGGCCAAA 
GAATTAAGAG AACTTCGGAT TGAAGAAACA AACCGCCCAC TGAAGAAGGT AACAGATTAC 
TCCTCCTCCA GTGAGGAGTC AGAAAGCAGT GATGAAGAGG AGGAAGATGG AGAGAGTGAG 
ACCCAGGATG GGACGGTGGC TGTCAGCGAC ATACCCAGAC TGATACCCAC AGGAGCCCCC 
GGGAGCGCTG AACAGTACAA TGTGGGAATG GTCGGCACAC ACGCGCTGGA GACCTCTCAT 
GCAGATACTT TCGGCAGCAG TATTTCAAGA GAAGGAACCT TGATGATAAG AGAACCGTCG 
GGAGAGAAGA AGCGACCTGG CCACAGTGAC AGCAATGGCT TTGCCGGCCA CATCAACCTC 
CCGGACCTGG TACAGCAGAG CCATTCCCCT GCGGGGACCC CCACCGAGGG CCTGGGGCGC 
GTCTCCACCC ACCCCCAGGA AATGGACGCC GGGACTGAAT ATGGCCTGGG GAGCAGCACC 
AAAGCCTCCT TCACCCCCTT TGTGGACCCC AGGGTATACC AGACATCTCC CACTGATGAA 
GATGAAGAGG ATGAGGAATC ATCAGCTGCG GGAACCCAGA GGTATCCAGG GGCCCTGGGA 
AAAGCTGTTC CTCCGAGATC TCCAAACTCA CCACCTATTT CTCAAAAGCA GTTTCTGGCT 
CTGTTTACTA GCGAACTTCT TAGGCAAGAA CAGGCCAAAC TAAATGAAGC AAGAAAGATT 
TCAGTGGTAA ATGTAAACCC AACCAACATT CGGCCTCATA GCGACACACC GGAGATCAGA 
AAATATAAGA AGCGATTCAA TTCAGAAATA CTTTGTGCAG CTCTGTGGGG TGTAAACCTC 
CTGGTGGGAA CCGAAAATGG CCTGATGCTT TTGGACCGAA GTGGGCAAGG CAAAGTTTAT 
AATCTGATCA ACCGGAGGCG ATTTCAGCAG ATGGATGTGC TCGAGGGACT GAATGTCCTC 
GTGACAATAT CAGGAAAGAA GAATAAGTTA CGAGTTTACT ATCTTTCATG GTTAAGAAAC 
AGAATACTAC ACAATGACCC AGAAGTAGAA AAGAAACAAG GCTGGATCAC TGTGGGAGAC 
TTGGAAGGCT GTATACATTA CAAAGTTGTT AAATATGAAA GGATCAAATT CTTGGTGATT 
GCCTTAAAGA ATGCTGTGGA GATATATGCT TGGGCTCCTA AACCGTATCA TAAGTTCATG 
GCATTTAAGT CTTTTGCAGA TCTCCAGCAC AAGCCACTGC TTGTTGATCT CACAGTAGAA 
GAAGGTCAAA GATTAAAGGT TATTTTTGGT TCACACACTG GTTTCCATGT AATTGATGTT 
GATTCAGGAA ACTCTTATGA TATCTACATA CCATCTCATA TTCAGGGCAA TATCACTCCT 
CATGCCATTG TCATCTTGCC TAAGACAGAT GGAATGGAAA TGCTTGTTTG CTATGAGGAT 
GAGGGGGTGT ATGTAAACAC CTACGGCCGG ATAACTAAGG ATGTGGTGCT CCAATGGGGA 
GAAATGCCCA CGTCTGTGGC CTACATTCAT TCCAATCAGA TAATGGGCTG GGGCGAGAAA 
GCTATTGAGA TCCGGTCAGT GGAAACAGGA CATTTGGATG GAGTATTTAT GCATAAGCGA 
GCTCAAAGGT TAAAGTTTCT ATGTGAAAGA AATGATAAGG TATTTTTTGC ATCCGTGCGA 
TCTGGAGGAA GTAGCCAAGT GTTTTTCATG ACCCTCAACA GAAATTCCAT GATGAACTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822684.1
CDS	341..4423
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAGTQRYPGALGKAVPPRSPNSPPISQKQFLALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_010824382.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824382.1

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAATATAA GCGCAAGCAG TTGGAAGAAC AGAGACAAGC AGAAAGGCTA 
CAGAGACAAC TGAAGCAAGA GAGAGACTAC TTGGTTTCTC TTCAGCATCA GCGGCAGGAG 
CAGAGGCCTG TGGAGAAGAA GCCACTGTAT CATTACAAAG AAGGGATGAG TCCTAGTGAG 
AAACCAGCAT GGGCCAAGGA GGTAGAAGAA CGGTCAAGGC TCAACCGGCA GAGTTCACCT 
GCCATGCCTC ACAAGGTTGC CAACAGGATA TCGGACCCCA ACCTGCCCCC ACGGTCGGAG 
TCCTTCAGCA TCAGTGGGGT TCAGCCTGCT CGGACACCCC CCATGCTCAG ATCAGTCGAT 
CCCCAGATCC CGCATCTGGT AGCAGTGAAA TCCCAGGGAC CTGCCCTGAC CGCCTCCCAG 
TCAGTGCATG AGCAGCCCAC GAAGGGCCTG TCTGGGTTTC AGGAGGCTCT GAATGTGAGC 
TCTCACCGCG TCGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCTGAAAA CCCACCTCTC 
CCCACTCGCA TTGAGAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA 
CCAAAGGTGC CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCAAGGAA GAATTCTCCT 
GGAAATGGTA GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCC 
GACCTGCGGA GAACCGAGCC CGTCTTGGAG AGCCCCTTGC AGAGGACGAG CAGTGGCAGT 
TCTTCCAGCT CCAGCACCCC CAGCTCCCAG CCCAGCTCCC AAGGAGGCTC TCAGCCTGGG 
TCACAAGCAG GATCCAGCGA GCGGACCCGG GTTCGAGCCA ACAGTAAGTC GGAAGGATCA 
CCTGTGCTCC CCCATGAGCC CTCCAAGGTG AAACCAGAAG AATCCAGGGA AATTACCCGA 
CCAAGTCGCC CAGCTAGCTA TAAGAAAGCT ATAGATGAGG ATCTGACGGC ATTGGCCAAA 
GAATTAAGAG AACTTCGGAT TGAAGAAACA AACCGCCCAC TGAAGAAGGT AACAGATTAC 
TCCTCCTCCA GTGAGGAGTC AGAAAGCAGT GATGAAGAGG AGGAAGATGG AGAGAGTGAG 
ACCCAGGATG GGACGGTGGC TGTCAGCGAC ATACCCAGAC TGATACCCAC AGGAGCCCCC 
GGGAGCGCTG AACAGTACAA TGTGGGAATG GTCGGCACAC ACGCGCTGGA GACCTCTCAT 
GCAGATACTT TCGGCAGCAG TATTTCAAGA GAAGGAACCT TGATGATAAG AGAACCGTCG 
GGAGAGAAGA AGCGACCTGG CCACAGTGAC AGCAATGGCT TTGCCGGCCA CATCAACCTC 
CCGGACCTGG TACAGCAGAG CCATTCCCCT GCGGGGACCC CCACCGAGGG CCTGGGGCGC 
GTCTCCACCC ACCCCCAGGA AATGGACGCC GGGACTGAAT ATGGCCTGGG GAGCAGCACC 
AAAGCCTCCT TCACCCCCTT TGTGGACCCC AGGGTATACC AGACATCTCC CACTGATGAA 
GATGAAGAGG ATGAGGAATC ATCAGCTGCG GGAACCCAGA GGTATCCAGG GGCCCTGGGA 
AAAGCTGTTC CTCCGAGATC TCCAAACTCA CCACCTATTT CTCAAAAGCA GTTTCTGGCT 
CTGTTTACTA GCGAACTTCT TAGGCAAGAA CAGGCCAAAC TAAATGAAGC AAGAAAGATT 
TCAGTGGTAA ATGTAAACCC AACCAACATT CGGCCTCATA GCGACACACC GGAGATCAGA 
AAATATAAGA AGCGATTCAA TTCAGAAATA CTTTGTGCAG CTCTGTGGGG TGTAAACCTC 
CTGGTGGGAA CCGAAAATGG CCTGATGCTT TTGGACCGAA GTGGGCAAGG CAAAGTTTAT 
AATCTGATCA ACCGGAGGCG ATTTCAGCAG ATGGATGTGC TCGAGGGACT GAATGTCCTC 
GTGACAATAT CAGGAAAGAA GAATAAGTTA CGAGTTTACT ATCTTTCATG GTTAAGAAAC 
AGAATACTAC ACAATGACCC AGAAGTAGAA AAGAAACAAG GCTGGATCAC TGTGGGAGAC 
TTGGAAGGCT GTATACATTA CAAAGTTGTT AAATATGAAA GGATCAAATT CTTGGTGATT 
GCCTTAAAGA ATGCTGTGGA GATATATGCT TGGGCTCCTA AACCGTATCA TAAGTTCATG 
GCATTTAAGT CTTTTGCAGA TCTCCAGCAC AAGCCACTGC TTGTTGATCT CACAGTAGAA 
GAAGGTCAAA GATTAAAGGT TATTTTTGGT TCACACACTG GTTTCCATGT AATTGATGTT 
GATTCAGGAA ACTCTTATGA TATCTACATA CCATCTCATA TTCAGGGCAA TATCACTCCT 
CATGCCATTG TCATCTTGCC TAAGACAGAT GGAATGGAAA TGCTTGTTTG CTATGAGGAT 
GAGGGGGTGT ATGTAAACAC CTACGGCCGG ATAACTAAGG ATGTGGTGCT CCAATGGGGA 
GAAATGCCCA CGTCTGTGGC CTACATTCAT TCCAATCAGA TAATGGGCTG GGGCGAGAAA 
GCTATTGAGA TCCGGTCAGT GGAAACAGGA CATTTGGATG GAGTATTTAT GCATAAGCGA 
GCTCAAAGGT TAAAGTTTCT ATGTGAAAGA AATGATAAGG TATTTTTTGC ATCCGTGCGA 
TCTGGAGGAA GTAGCCAAGT GTTTTTCATG ACCCTCAACA GAAATTCCAT GATGAACTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_010801394.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799696.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X5, mRNA.

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CloneID	OBa54386
Clone ID Related Accession (Same CDS sequence)	XM_010801394.1 , XM_010824383.1
Accession Version	XM_010801394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGGAGGCTC TGAATGTGAG CTCTCACCGC 
GTCGAGATGC CACGCCAGAA CTCAGATCCC ACCTCTGAAA ACCCACCTCT CCCCACTCGC 
ATTGAGAAGT TTGACCGAAG CTCTTGGTTA CGACAGGAAG AAGACATTCC ACCAAAGGTG 
CCTCAAAGAA CAACTTCTAT ATCCCCAGCA TTAGCAAGGA AGAATTCTCC TGGAAATGGT 
AGTGCTCTGG GACCCAGACT AGGATCTCAA CCCATCAGAG CAAGCAACCC CGACCTGCGG 
AGAACCGAGC CCGTCTTGGA GAGCCCCTTG CAGAGGACGA GCAGTGGCAG TTCTTCCAGC 
TCCAGCACCC CCAGCTCCCA GCCCAGCTCC CAAGGAGGCT CTCAGCCTGG GTCACAAGCA 
GGATCCAGCG AGCGGACCCG GGTTCGAGCC AACAGTAAGT CGGAAGGATC ACCTGTGCTC 
CCCCATGAGC CCTCCAAGGT GAAACCAGAA GAATCCAGGG AAATTACCCG ACCAAGTCGC 
CCAGCTAGCT ATAAGAAAGC TATAGATGAG GATCTGACGG CATTGGCCAA AGAATTAAGA 
GAACTTCGGA TTGAAGAAAC AAACCGCCCA CTGAAGAAGG TAACAGATTA CTCCTCCTCC 
AGTGAGGAGT CAGAAAGCAG TGATGAAGAG GAGGAAGATG GAGAGAGTGA GACCCAGGAT 
GGGACGGTGG CTGTCAGCGA CATACCCAGA CTGATACCCA CAGGAGCCCC CGGGAGCGCT 
GAACAGTACA ATGTGGGAAT GGTCGGCACA CACGCGCTGG AGACCTCTCA TGCAGATACT 
TTCGGCAGCA GTATTTCAAG AGAAGGAACC TTGATGATAA GAGAACCGTC GGGAGAGAAG 
AAGCGACCTG GCCACAGTGA CAGCAATGGC TTTGCCGGCC ACATCAACCT CCCGGACCTG 
GTACAGCAGA GCCATTCCCC TGCGGGGACC CCCACCGAGG GCCTGGGGCG CGTCTCCACC 
CACCCCCAGG AAATGGACGC CGGGACTGAA TATGGCCTGG GGAGCAGCAC CAAAGCCTCC 
TTCACCCCCT TTGTGGACCC CAGGGTATAC CAGACATCTC CCACTGATGA AGATGAAGAG 
GATGAGGAAT CATCAGCTGC GGGAACCCAG AGGTATCCAG GGGCCCTGGG AAAAGCTGTT 
CCTCCGAGAT CTCCAAACTC ACCACCTATT TCTCAAAAGC AGTTTCTGGC TCTGTTTACT 
AGCGAACTTC TTAGGCAAGA ACAGGCCAAA CTAAATGAAG CAAGAAAGAT TTCAGTGGTA 
AATGTAAACC CAACCAACAT TCGGCCTCAT AGCGACACAC CGGAGATCAG AAAATATAAG 
AAGCGATTCA ATTCAGAAAT ACTTTGTGCA GCTCTGTGGG GTGTAAACCT CCTGGTGGGA 
ACCGAAAATG GCCTGATGCT TTTGGACCGA AGTGGGCAAG GCAAAGTTTA TAATCTGATC 
AACCGGAGGC GATTTCAGCA GATGGATGTG CTCGAGGGAC TGAATGTCCT CGTGACAATA 
TCAGGAAAGA AGAATAAGTT ACGAGTTTAC TATCTTTCAT GGTTAAGAAA CAGAATACTA 
CACAATGACC CAGAAGTAGA AAAGAAACAA GGCTGGATCA CTGTGGGAGA CTTGGAAGGC 
TGTATACATT ACAAAGTTGT TAAATATGAA AGGATCAAAT TCTTGGTGAT TGCCTTAAAG 
AATGCTGTGG AGATATATGC TTGGGCTCCT AAACCGTATC ATAAGTTCAT GGCATTTAAG 
TCTTTTGCAG ATCTCCAGCA CAAGCCACTG CTTGTTGATC TCACAGTAGA AGAAGGTCAA 
AGATTAAAGG TTATTTTTGG TTCACACACT GGTTTCCATG TAATTGATGT TGATTCAGGA 
AACTCTTATG ATATCTACAT ACCATCTCAT ATTCAGGGCA ATATCACTCC TCATGCCATT 
GTCATCTTGC CTAAGACAGA TGGAATGGAA ATGCTTGTTT GCTATGAGGA TGAGGGGGTG 
TATGTAAACA CCTACGGCCG GATAACTAAG GATGTGGTGC TCCAATGGGG AGAAATGCCC 
ACGTCTGTGG CCTACATTCA TTCCAATCAG ATAATGGGCT GGGGCGAGAA AGCTATTGAG 
ATCCGGTCAG TGGAAACAGG ACATTTGGAT GGAGTATTTA TGCATAAGCG AGCTCAAAGG 
TTAAAGTTTC TATGTGAAAG AAATGATAAG GTATTTTTTG CATCCGTGCG ATCTGGAGGA 
AGTAGCCAAG TGTTTTTCAT GACCCTCAAC AGAAATTCCA TGATGAACTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799696.1
CDS	341..4414
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAGTQRYPGALGKAVPPRSPNSPPISQKQFLALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_010801394.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801394.1

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGGAGGCTC TGAATGTGAG CTCTCACCGC 
GTCGAGATGC CACGCCAGAA CTCAGATCCC ACCTCTGAAA ACCCACCTCT CCCCACTCGC 
ATTGAGAAGT TTGACCGAAG CTCTTGGTTA CGACAGGAAG AAGACATTCC ACCAAAGGTG 
CCTCAAAGAA CAACTTCTAT ATCCCCAGCA TTAGCAAGGA AGAATTCTCC TGGAAATGGT 
AGTGCTCTGG GACCCAGACT AGGATCTCAA CCCATCAGAG CAAGCAACCC CGACCTGCGG 
AGAACCGAGC CCGTCTTGGA GAGCCCCTTG CAGAGGACGA GCAGTGGCAG TTCTTCCAGC 
TCCAGCACCC CCAGCTCCCA GCCCAGCTCC CAAGGAGGCT CTCAGCCTGG GTCACAAGCA 
GGATCCAGCG AGCGGACCCG GGTTCGAGCC AACAGTAAGT CGGAAGGATC ACCTGTGCTC 
CCCCATGAGC CCTCCAAGGT GAAACCAGAA GAATCCAGGG AAATTACCCG ACCAAGTCGC 
CCAGCTAGCT ATAAGAAAGC TATAGATGAG GATCTGACGG CATTGGCCAA AGAATTAAGA 
GAACTTCGGA TTGAAGAAAC AAACCGCCCA CTGAAGAAGG TAACAGATTA CTCCTCCTCC 
AGTGAGGAGT CAGAAAGCAG TGATGAAGAG GAGGAAGATG GAGAGAGTGA GACCCAGGAT 
GGGACGGTGG CTGTCAGCGA CATACCCAGA CTGATACCCA CAGGAGCCCC CGGGAGCGCT 
GAACAGTACA ATGTGGGAAT GGTCGGCACA CACGCGCTGG AGACCTCTCA TGCAGATACT 
TTCGGCAGCA GTATTTCAAG AGAAGGAACC TTGATGATAA GAGAACCGTC GGGAGAGAAG 
AAGCGACCTG GCCACAGTGA CAGCAATGGC TTTGCCGGCC ACATCAACCT CCCGGACCTG 
GTACAGCAGA GCCATTCCCC TGCGGGGACC CCCACCGAGG GCCTGGGGCG CGTCTCCACC 
CACCCCCAGG AAATGGACGC CGGGACTGAA TATGGCCTGG GGAGCAGCAC CAAAGCCTCC 
TTCACCCCCT TTGTGGACCC CAGGGTATAC CAGACATCTC CCACTGATGA AGATGAAGAG 
GATGAGGAAT CATCAGCTGC GGGAACCCAG AGGTATCCAG GGGCCCTGGG AAAAGCTGTT 
CCTCCGAGAT CTCCAAACTC ACCACCTATT TCTCAAAAGC AGTTTCTGGC TCTGTTTACT 
AGCGAACTTC TTAGGCAAGA ACAGGCCAAA CTAAATGAAG CAAGAAAGAT TTCAGTGGTA 
AATGTAAACC CAACCAACAT TCGGCCTCAT AGCGACACAC CGGAGATCAG AAAATATAAG 
AAGCGATTCA ATTCAGAAAT ACTTTGTGCA GCTCTGTGGG GTGTAAACCT CCTGGTGGGA 
ACCGAAAATG GCCTGATGCT TTTGGACCGA AGTGGGCAAG GCAAAGTTTA TAATCTGATC 
AACCGGAGGC GATTTCAGCA GATGGATGTG CTCGAGGGAC TGAATGTCCT CGTGACAATA 
TCAGGAAAGA AGAATAAGTT ACGAGTTTAC TATCTTTCAT GGTTAAGAAA CAGAATACTA 
CACAATGACC CAGAAGTAGA AAAGAAACAA GGCTGGATCA CTGTGGGAGA CTTGGAAGGC 
TGTATACATT ACAAAGTTGT TAAATATGAA AGGATCAAAT TCTTGGTGAT TGCCTTAAAG 
AATGCTGTGG AGATATATGC TTGGGCTCCT AAACCGTATC ATAAGTTCAT GGCATTTAAG 
TCTTTTGCAG ATCTCCAGCA CAAGCCACTG CTTGTTGATC TCACAGTAGA AGAAGGTCAA 
AGATTAAAGG TTATTTTTGG TTCACACACT GGTTTCCATG TAATTGATGT TGATTCAGGA 
AACTCTTATG ATATCTACAT ACCATCTCAT ATTCAGGGCA ATATCACTCC TCATGCCATT 
GTCATCTTGC CTAAGACAGA TGGAATGGAA ATGCTTGTTT GCTATGAGGA TGAGGGGGTG 
TATGTAAACA CCTACGGCCG GATAACTAAG GATGTGGTGC TCCAATGGGG AGAAATGCCC 
ACGTCTGTGG CCTACATTCA TTCCAATCAG ATAATGGGCT GGGGCGAGAA AGCTATTGAG 
ATCCGGTCAG TGGAAACAGG ACATTTGGAT GGAGTATTTA TGCATAAGCG AGCTCAAAGG 
TTAAAGTTTC TATGTGAAAG AAATGATAAG GTATTTTTTG CATCCGTGCG ATCTGGAGGA 
AGTAGCCAAG TGTTTTTCAT GACCCTCAAC AGAAATTCCA TGATGAACTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_010824383.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822685.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa54386
Clone ID Related Accession (Same CDS sequence)	XM_010801394.1 , XM_010824383.1
Accession Version	XM_010824383.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644547.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGGAGGCTC TGAATGTGAG CTCTCACCGC 
GTCGAGATGC CACGCCAGAA CTCAGATCCC ACCTCTGAAA ACCCACCTCT CCCCACTCGC 
ATTGAGAAGT TTGACCGAAG CTCTTGGTTA CGACAGGAAG AAGACATTCC ACCAAAGGTG 
CCTCAAAGAA CAACTTCTAT ATCCCCAGCA TTAGCAAGGA AGAATTCTCC TGGAAATGGT 
AGTGCTCTGG GACCCAGACT AGGATCTCAA CCCATCAGAG CAAGCAACCC CGACCTGCGG 
AGAACCGAGC CCGTCTTGGA GAGCCCCTTG CAGAGGACGA GCAGTGGCAG TTCTTCCAGC 
TCCAGCACCC CCAGCTCCCA GCCCAGCTCC CAAGGAGGCT CTCAGCCTGG GTCACAAGCA 
GGATCCAGCG AGCGGACCCG GGTTCGAGCC AACAGTAAGT CGGAAGGATC ACCTGTGCTC 
CCCCATGAGC CCTCCAAGGT GAAACCAGAA GAATCCAGGG AAATTACCCG ACCAAGTCGC 
CCAGCTAGCT ATAAGAAAGC TATAGATGAG GATCTGACGG CATTGGCCAA AGAATTAAGA 
GAACTTCGGA TTGAAGAAAC AAACCGCCCA CTGAAGAAGG TAACAGATTA CTCCTCCTCC 
AGTGAGGAGT CAGAAAGCAG TGATGAAGAG GAGGAAGATG GAGAGAGTGA GACCCAGGAT 
GGGACGGTGG CTGTCAGCGA CATACCCAGA CTGATACCCA CAGGAGCCCC CGGGAGCGCT 
GAACAGTACA ATGTGGGAAT GGTCGGCACA CACGCGCTGG AGACCTCTCA TGCAGATACT 
TTCGGCAGCA GTATTTCAAG AGAAGGAACC TTGATGATAA GAGAACCGTC GGGAGAGAAG 
AAGCGACCTG GCCACAGTGA CAGCAATGGC TTTGCCGGCC ACATCAACCT CCCGGACCTG 
GTACAGCAGA GCCATTCCCC TGCGGGGACC CCCACCGAGG GCCTGGGGCG CGTCTCCACC 
CACCCCCAGG AAATGGACGC CGGGACTGAA TATGGCCTGG GGAGCAGCAC CAAAGCCTCC 
TTCACCCCCT TTGTGGACCC CAGGGTATAC CAGACATCTC CCACTGATGA AGATGAAGAG 
GATGAGGAAT CATCAGCTGC GGGAACCCAG AGGTATCCAG GGGCCCTGGG AAAAGCTGTT 
CCTCCGAGAT CTCCAAACTC ACCACCTATT TCTCAAAAGC AGTTTCTGGC TCTGTTTACT 
AGCGAACTTC TTAGGCAAGA ACAGGCCAAA CTAAATGAAG CAAGAAAGAT TTCAGTGGTA 
AATGTAAACC CAACCAACAT TCGGCCTCAT AGCGACACAC CGGAGATCAG AAAATATAAG 
AAGCGATTCA ATTCAGAAAT ACTTTGTGCA GCTCTGTGGG GTGTAAACCT CCTGGTGGGA 
ACCGAAAATG GCCTGATGCT TTTGGACCGA AGTGGGCAAG GCAAAGTTTA TAATCTGATC 
AACCGGAGGC GATTTCAGCA GATGGATGTG CTCGAGGGAC TGAATGTCCT CGTGACAATA 
TCAGGAAAGA AGAATAAGTT ACGAGTTTAC TATCTTTCAT GGTTAAGAAA CAGAATACTA 
CACAATGACC CAGAAGTAGA AAAGAAACAA GGCTGGATCA CTGTGGGAGA CTTGGAAGGC 
TGTATACATT ACAAAGTTGT TAAATATGAA AGGATCAAAT TCTTGGTGAT TGCCTTAAAG 
AATGCTGTGG AGATATATGC TTGGGCTCCT AAACCGTATC ATAAGTTCAT GGCATTTAAG 
TCTTTTGCAG ATCTCCAGCA CAAGCCACTG CTTGTTGATC TCACAGTAGA AGAAGGTCAA 
AGATTAAAGG TTATTTTTGG TTCACACACT GGTTTCCATG TAATTGATGT TGATTCAGGA 
AACTCTTATG ATATCTACAT ACCATCTCAT ATTCAGGGCA ATATCACTCC TCATGCCATT 
GTCATCTTGC CTAAGACAGA TGGAATGGAA ATGCTTGTTT GCTATGAGGA TGAGGGGGTG 
TATGTAAACA CCTACGGCCG GATAACTAAG GATGTGGTGC TCCAATGGGG AGAAATGCCC 
ACGTCTGTGG CCTACATTCA TTCCAATCAG ATAATGGGCT GGGGCGAGAA AGCTATTGAG 
ATCCGGTCAG TGGAAACAGG ACATTTGGAT GGAGTATTTA TGCATAAGCG AGCTCAAAGG 
TTAAAGTTTC TATGTGAAAG AAATGATAAG GTATTTTTTG CATCCGTGCG ATCTGGAGGA 
AGTAGCCAAG TGTTTTTCAT GACCCTCAAC AGAAATTCCA TGATGAACTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822685.1
CDS	341..4414
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREPSGEKKRPGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDAGTEYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAGTQRYPGALGKAVPPRSPNSPPISQKQFLALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_010824383.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824383.1

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGGAGGCTC TGAATGTGAG CTCTCACCGC 
GTCGAGATGC CACGCCAGAA CTCAGATCCC ACCTCTGAAA ACCCACCTCT CCCCACTCGC 
ATTGAGAAGT TTGACCGAAG CTCTTGGTTA CGACAGGAAG AAGACATTCC ACCAAAGGTG 
CCTCAAAGAA CAACTTCTAT ATCCCCAGCA TTAGCAAGGA AGAATTCTCC TGGAAATGGT 
AGTGCTCTGG GACCCAGACT AGGATCTCAA CCCATCAGAG CAAGCAACCC CGACCTGCGG 
AGAACCGAGC CCGTCTTGGA GAGCCCCTTG CAGAGGACGA GCAGTGGCAG TTCTTCCAGC 
TCCAGCACCC CCAGCTCCCA GCCCAGCTCC CAAGGAGGCT CTCAGCCTGG GTCACAAGCA 
GGATCCAGCG AGCGGACCCG GGTTCGAGCC AACAGTAAGT CGGAAGGATC ACCTGTGCTC 
CCCCATGAGC CCTCCAAGGT GAAACCAGAA GAATCCAGGG AAATTACCCG ACCAAGTCGC 
CCAGCTAGCT ATAAGAAAGC TATAGATGAG GATCTGACGG CATTGGCCAA AGAATTAAGA 
GAACTTCGGA TTGAAGAAAC AAACCGCCCA CTGAAGAAGG TAACAGATTA CTCCTCCTCC 
AGTGAGGAGT CAGAAAGCAG TGATGAAGAG GAGGAAGATG GAGAGAGTGA GACCCAGGAT 
GGGACGGTGG CTGTCAGCGA CATACCCAGA CTGATACCCA CAGGAGCCCC CGGGAGCGCT 
GAACAGTACA ATGTGGGAAT GGTCGGCACA CACGCGCTGG AGACCTCTCA TGCAGATACT 
TTCGGCAGCA GTATTTCAAG AGAAGGAACC TTGATGATAA GAGAACCGTC GGGAGAGAAG 
AAGCGACCTG GCCACAGTGA CAGCAATGGC TTTGCCGGCC ACATCAACCT CCCGGACCTG 
GTACAGCAGA GCCATTCCCC TGCGGGGACC CCCACCGAGG GCCTGGGGCG CGTCTCCACC 
CACCCCCAGG AAATGGACGC CGGGACTGAA TATGGCCTGG GGAGCAGCAC CAAAGCCTCC 
TTCACCCCCT TTGTGGACCC CAGGGTATAC CAGACATCTC CCACTGATGA AGATGAAGAG 
GATGAGGAAT CATCAGCTGC GGGAACCCAG AGGTATCCAG GGGCCCTGGG AAAAGCTGTT 
CCTCCGAGAT CTCCAAACTC ACCACCTATT TCTCAAAAGC AGTTTCTGGC TCTGTTTACT 
AGCGAACTTC TTAGGCAAGA ACAGGCCAAA CTAAATGAAG CAAGAAAGAT TTCAGTGGTA 
AATGTAAACC CAACCAACAT TCGGCCTCAT AGCGACACAC CGGAGATCAG AAAATATAAG 
AAGCGATTCA ATTCAGAAAT ACTTTGTGCA GCTCTGTGGG GTGTAAACCT CCTGGTGGGA 
ACCGAAAATG GCCTGATGCT TTTGGACCGA AGTGGGCAAG GCAAAGTTTA TAATCTGATC 
AACCGGAGGC GATTTCAGCA GATGGATGTG CTCGAGGGAC TGAATGTCCT CGTGACAATA 
TCAGGAAAGA AGAATAAGTT ACGAGTTTAC TATCTTTCAT GGTTAAGAAA CAGAATACTA 
CACAATGACC CAGAAGTAGA AAAGAAACAA GGCTGGATCA CTGTGGGAGA CTTGGAAGGC 
TGTATACATT ACAAAGTTGT TAAATATGAA AGGATCAAAT TCTTGGTGAT TGCCTTAAAG 
AATGCTGTGG AGATATATGC TTGGGCTCCT AAACCGTATC ATAAGTTCAT GGCATTTAAG 
TCTTTTGCAG ATCTCCAGCA CAAGCCACTG CTTGTTGATC TCACAGTAGA AGAAGGTCAA 
AGATTAAAGG TTATTTTTGG TTCACACACT GGTTTCCATG TAATTGATGT TGATTCAGGA 
AACTCTTATG ATATCTACAT ACCATCTCAT ATTCAGGGCA ATATCACTCC TCATGCCATT 
GTCATCTTGC CTAAGACAGA TGGAATGGAA ATGCTTGTTT GCTATGAGGA TGAGGGGGTG 
TATGTAAACA CCTACGGCCG GATAACTAAG GATGTGGTGC TCCAATGGGG AGAAATGCCC 
ACGTCTGTGG CCTACATTCA TTCCAATCAG ATAATGGGCT GGGGCGAGAA AGCTATTGAG 
ATCCGGTCAG TGGAAACAGG ACATTTGGAT GGAGTATTTA TGCATAAGCG AGCTCAAAGG 
TTAAAGTTTC TATGTGAAAG AAATGATAAG GTATTTTTTG CATCCGTGCG ATCTGGAGGA 
AGTAGCCAAG TGTTTTTCAT GACCCTCAAC AGAAATTCCA TGATGAACTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_010801395.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799697.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa54387
Clone ID Related Accession (Same CDS sequence)	XM_010801395.1 , XM_010824384.1
Accession Version	XM_010801395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ATATGGCCTG GGGAGCAGCA CCAAAGCCTC CTTCACCCCC 
TTTGTGGACC CCAGGGTATA CCAGACATCT CCCACTGATG AAGATGAAGA GGATGAGGAA 
TCATCAGCTG CGGGAACCCA GAGGTATCCA GGGGCCCTGG GAAAAGCTGT TCCTCCGAGA 
TCTCCAAACT CACCACCTAT TTCTCAAAAG CAGTTTCTGG CTCTGTTTAC TAGCGAACTT 
CTTAGGCAAG AACAGGCCAA ACTAAATGAA GCAAGAAAGA TTTCAGTGGT AAATGTAAAC 
CCAACCAACA TTCGGCCTCA TAGCGACACA CCGGAGATCA GAAAATATAA GAAGCGATTC 
AATTCAGAAA TACTTTGTGC AGCTCTGTGG GGTGTAAACC TCCTGGTGGG AACCGAAAAT 
GGCCTGATGC TTTTGGACCG AAGTGGGCAA GGCAAAGTTT ATAATCTGAT CAACCGGAGG 
CGATTTCAGC AGATGGATGT GCTCGAGGGA CTGAATGTCC TCGTGACAAT ATCAGGAAAG 
AAGAATAAGT TACGAGTTTA CTATCTTTCA TGGTTAAGAA ACAGAATACT ACACAATGAC 
CCAGAAGTAG AAAAGAAACA AGGCTGGATC ACTGTGGGAG ACTTGGAAGG CTGTATACAT 
TACAAAGTTG TTAAATATGA AAGGATCAAA TTCTTGGTGA TTGCCTTAAA GAATGCTGTG 
GAGATATATG CTTGGGCTCC TAAACCGTAT CATAAGTTCA TGGCATTTAA GTCTTTTGCA 
GATCTCCAGC ACAAGCCACT GCTTGTTGAT CTCACAGTAG AAGAAGGTCA AAGATTAAAG 
GTTATTTTTG GTTCACACAC TGGTTTCCAT GTAATTGATG TTGATTCAGG AAACTCTTAT 
GATATCTACA TACCATCTCA TATTCAGGGC AATATCACTC CTCATGCCAT TGTCATCTTG 
CCTAAGACAG ATGGAATGGA AATGCTTGTT TGCTATGAGG ATGAGGGGGT GTATGTAAAC 
ACCTACGGCC GGATAACTAA GGATGTGGTG CTCCAATGGG GAGAAATGCC CACGTCTGTG 
GCCTACATTC ATTCCAATCA GATAATGGGC TGGGGCGAGA AAGCTATTGA GATCCGGTCA 
GTGGAAACAG GACATTTGGA TGGAGTATTT ATGCATAAGC GAGCTCAAAG GTTAAAGTTT 
CTATGTGAAA GAAATGATAA GGTATTTTTT GCATCCGTGC GATCTGGAGG AAGTAGCCAA 
GTGTTTTTCA TGACCCTCAA CAGAAATTCC ATGATGAACT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799697.1
CDS	341..4345
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAGTQRYPGALGKAVPPRSPNSPPISQKQFLALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_010801395.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801395.1

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ATATGGCCTG GGGAGCAGCA CCAAAGCCTC CTTCACCCCC 
TTTGTGGACC CCAGGGTATA CCAGACATCT CCCACTGATG AAGATGAAGA GGATGAGGAA 
TCATCAGCTG CGGGAACCCA GAGGTATCCA GGGGCCCTGG GAAAAGCTGT TCCTCCGAGA 
TCTCCAAACT CACCACCTAT TTCTCAAAAG CAGTTTCTGG CTCTGTTTAC TAGCGAACTT 
CTTAGGCAAG AACAGGCCAA ACTAAATGAA GCAAGAAAGA TTTCAGTGGT AAATGTAAAC 
CCAACCAACA TTCGGCCTCA TAGCGACACA CCGGAGATCA GAAAATATAA GAAGCGATTC 
AATTCAGAAA TACTTTGTGC AGCTCTGTGG GGTGTAAACC TCCTGGTGGG AACCGAAAAT 
GGCCTGATGC TTTTGGACCG AAGTGGGCAA GGCAAAGTTT ATAATCTGAT CAACCGGAGG 
CGATTTCAGC AGATGGATGT GCTCGAGGGA CTGAATGTCC TCGTGACAAT ATCAGGAAAG 
AAGAATAAGT TACGAGTTTA CTATCTTTCA TGGTTAAGAA ACAGAATACT ACACAATGAC 
CCAGAAGTAG AAAAGAAACA AGGCTGGATC ACTGTGGGAG ACTTGGAAGG CTGTATACAT 
TACAAAGTTG TTAAATATGA AAGGATCAAA TTCTTGGTGA TTGCCTTAAA GAATGCTGTG 
GAGATATATG CTTGGGCTCC TAAACCGTAT CATAAGTTCA TGGCATTTAA GTCTTTTGCA 
GATCTCCAGC ACAAGCCACT GCTTGTTGAT CTCACAGTAG AAGAAGGTCA AAGATTAAAG 
GTTATTTTTG GTTCACACAC TGGTTTCCAT GTAATTGATG TTGATTCAGG AAACTCTTAT 
GATATCTACA TACCATCTCA TATTCAGGGC AATATCACTC CTCATGCCAT TGTCATCTTG 
CCTAAGACAG ATGGAATGGA AATGCTTGTT TGCTATGAGG ATGAGGGGGT GTATGTAAAC 
ACCTACGGCC GGATAACTAA GGATGTGGTG CTCCAATGGG GAGAAATGCC CACGTCTGTG 
GCCTACATTC ATTCCAATCA GATAATGGGC TGGGGCGAGA AAGCTATTGA GATCCGGTCA 
GTGGAAACAG GACATTTGGA TGGAGTATTT ATGCATAAGC GAGCTCAAAG GTTAAAGTTT 
CTATGTGAAA GAAATGATAA GGTATTTTTT GCATCCGTGC GATCTGGAGG AAGTAGCCAA 
GTGTTTTTCA TGACCCTCAA CAGAAATTCC ATGATGAACT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNIK ( XM_010824384.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822686.1 Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X7, mRNA.

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CloneID	OBa54387
Clone ID Related Accession (Same CDS sequence)	XM_010801395.1 , XM_010824384.1
Accession Version	XM_010824384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRAF2 and NCK-interacting protein kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644547.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ATATGGCCTG GGGAGCAGCA CCAAAGCCTC CTTCACCCCC 
TTTGTGGACC CCAGGGTATA CCAGACATCT CCCACTGATG AAGATGAAGA GGATGAGGAA 
TCATCAGCTG CGGGAACCCA GAGGTATCCA GGGGCCCTGG GAAAAGCTGT TCCTCCGAGA 
TCTCCAAACT CACCACCTAT TTCTCAAAAG CAGTTTCTGG CTCTGTTTAC TAGCGAACTT 
CTTAGGCAAG AACAGGCCAA ACTAAATGAA GCAAGAAAGA TTTCAGTGGT AAATGTAAAC 
CCAACCAACA TTCGGCCTCA TAGCGACACA CCGGAGATCA GAAAATATAA GAAGCGATTC 
AATTCAGAAA TACTTTGTGC AGCTCTGTGG GGTGTAAACC TCCTGGTGGG AACCGAAAAT 
GGCCTGATGC TTTTGGACCG AAGTGGGCAA GGCAAAGTTT ATAATCTGAT CAACCGGAGG 
CGATTTCAGC AGATGGATGT GCTCGAGGGA CTGAATGTCC TCGTGACAAT ATCAGGAAAG 
AAGAATAAGT TACGAGTTTA CTATCTTTCA TGGTTAAGAA ACAGAATACT ACACAATGAC 
CCAGAAGTAG AAAAGAAACA AGGCTGGATC ACTGTGGGAG ACTTGGAAGG CTGTATACAT 
TACAAAGTTG TTAAATATGA AAGGATCAAA TTCTTGGTGA TTGCCTTAAA GAATGCTGTG 
GAGATATATG CTTGGGCTCC TAAACCGTAT CATAAGTTCA TGGCATTTAA GTCTTTTGCA 
GATCTCCAGC ACAAGCCACT GCTTGTTGAT CTCACAGTAG AAGAAGGTCA AAGATTAAAG 
GTTATTTTTG GTTCACACAC TGGTTTCCAT GTAATTGATG TTGATTCAGG AAACTCTTAT 
GATATCTACA TACCATCTCA TATTCAGGGC AATATCACTC CTCATGCCAT TGTCATCTTG 
CCTAAGACAG ATGGAATGGA AATGCTTGTT TGCTATGAGG ATGAGGGGGT GTATGTAAAC 
ACCTACGGCC GGATAACTAA GGATGTGGTG CTCCAATGGG GAGAAATGCC CACGTCTGTG 
GCCTACATTC ATTCCAATCA GATAATGGGC TGGGGCGAGA AAGCTATTGA GATCCGGTCA 
GTGGAAACAG GACATTTGGA TGGAGTATTT ATGCATAAGC GAGCTCAAAG GTTAAAGTTT 
CTATGTGAAA GAAATGATAA GGTATTTTTT GCATCCGTGC GATCTGGAGG AAGTAGCCAA 
GTGTTTTTCA TGACCCTCAA CAGAAATTCC ATGATGAACT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822686.1
CDS	341..4345
Translation	MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRSVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVSSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESREITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESETQDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGTHALETSHADTFGSSISREGTLMIREYGLGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAGTQRYPGALGKAVPPRSPNSPPISQKQFLALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

Target ORF information:

RefSeq Version	XM_010824384.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TRAF2 and NCK interacting kinase (TNIK), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824384.1

ORF Insert Sequence:

ATGGCGAGCG ACTCCCCGGC TCGCAGCTTG GATGAAATAG ATCTCTCTGC TTTGAGGGAC 
CCCGCAGGAA TCTTTGAATT GGTGGAACTT GTTGGCAACG GAACATATGG ACAAGTATAT 
AAGGGTCGTC ATGTCAAAAC GGGCCAGCTG GCAGCCATCA AGGTTATGGA TGTCACAGGG 
GATGAAGAGG AAGAAATCAA ACAAGAAATT AACATGTTGA AGAAATACTC TCATCACCGG 
AATATAGCTA CATACTATGG TGCTTTTATC AAAAAGAACC CACCAGGCAT GGATGACCAA 
CTTTGGTTGG TGATGGAGTT TTGCGGTGCG GGCTCTGTCA CTGACCTGAT CAAGAACACA 
AAAGGTAACA CGTTGAAGGA GGAATGGATT GCGTACATCT GCAGGGAGAT CTTACGAGGG 
CTGAGTCACC TGCACCAGCA TAAAGTGATT CATCGAGATA TTAAAGGGCA GAATGTCTTG 
CTGACTGAAA ATGCAGAAGT GAAACTAGTG GACTTTGGAG TCAGTGCCCA GCTTGATCGA 
ACAGTGGGCA GGAGGAATAC CTTCATCGGA ACACCCTACT GGATGGCGCC AGAAGTTATT 
GCCTGCGATG AAAACCCAGA TGCCACGTAT GATTTCAAGA GTGACCTGTG GTCTCTGGGA 
ATCACCGCTA TCGAGATGGC AGAAGGTGCT CCCCCTCTGT GTGACATGCA CCCCATGAGA 
GCTCTCTTCC TCATCCCCCG GAACCCAGCG CCTCGGCTGA AGTCTAAGAA GTGGTCAAAA 
AAATTCCAGT CGTTTATTGA GAGCTGCTTG GTAAAGAACC ACAGCCAGCG ACCAGCAACG 
GAACAACTGA TGAAGCATCC ATTTATACGA GACCAGCCTA ATGAGCGACA GGTCCGCATT 
CAGCTCAAGG ACCATATCGA CAGAACAAAG AAGAAGCGAG GAGAGAAAGA TGAGACGGAG 
TATGAGTACA GCGGCAGTGA GGAAGAAGAA GAGGAGAATG ACTCCGGAGA GCCCAGCTCC 
ATTCTCAACC TGCCTGGGGA GTCAACGCTG CGGCGGGACT TCCTGAGGCT CCAGCTGGCC 
AACAAGGAGC GGTCTGAGGC CCTGCGGCGG CAGCAGCTGG AGCAGCAGCA GCGGGAGAAC 
GAGGAGCATA AGCGGCAGCT GCTGGCCGAG CGCCAGAAGC GCATCGAGGA GCAGAAGGAG 
CAGCGGCGGC GGCTGGAGGA GCAACAAAGG CGAGAGAAGG AGCTGCGGAA ACAGCAAGAG 
AGGGAGCAGC GCAGACACTA TGAGGAGCAG ATGCGCCGGG AGGAGGAGAG GCGGCGCGCA 
GAGCACGAGC AGGAGTACAT CAGGCGACAG CTGGAGGAGG AGCAGAGGCA GCTAGAGATC 
TTACAGCAGC AGCTACTGCA TGAACAAGCT CTACTTCTGG AATATAAGCG CAAGCAGTTG 
GAAGAACAGA GACAAGCAGA AAGGCTACAG AGACAACTGA AGCAAGAGAG AGACTACTTG 
GTTTCTCTTC AGCATCAGCG GCAGGAGCAG AGGCCTGTGG AGAAGAAGCC ACTGTATCAT 
TACAAAGAAG GGATGAGTCC TAGTGAGAAA CCAGCATGGG CCAAGGAGGT AGAAGAACGG 
TCAAGGCTCA ACCGGCAGAG TTCACCTGCC ATGCCTCACA AGGTTGCCAA CAGGATATCG 
GACCCCAACC TGCCCCCACG GTCGGAGTCC TTCAGCATCA GTGGGGTTCA GCCTGCTCGG 
ACACCCCCCA TGCTCAGATC AGTCGATCCC CAGATCCCGC ATCTGGTAGC AGTGAAATCC 
CAGGGACCTG CCCTGACCGC CTCCCAGTCA GTGCATGAGC AGCCCACGAA GGGCCTGTCT 
GGGTTTCAGG AGGCTCTGAA TGTGAGCTCT CACCGCGTCG AGATGCCACG CCAGAACTCA 
GATCCCACCT CTGAAAACCC ACCTCTCCCC ACTCGCATTG AGAAGTTTGA CCGAAGCTCT 
TGGTTACGAC AGGAAGAAGA CATTCCACCA AAGGTGCCTC AAAGAACAAC TTCTATATCC 
CCAGCATTAG CAAGGAAGAA TTCTCCTGGA AATGGTAGTG CTCTGGGACC CAGACTAGGA 
TCTCAACCCA TCAGAGCAAG CAACCCCGAC CTGCGGAGAA CCGAGCCCGT CTTGGAGAGC 
CCCTTGCAGA GGACGAGCAG TGGCAGTTCT TCCAGCTCCA GCACCCCCAG CTCCCAGCCC 
AGCTCCCAAG GAGGCTCTCA GCCTGGGTCA CAAGCAGGAT CCAGCGAGCG GACCCGGGTT 
CGAGCCAACA GTAAGTCGGA AGGATCACCT GTGCTCCCCC ATGAGCCCTC CAAGGTGAAA 
CCAGAAGAAT CCAGGGAAAT TACCCGACCA AGTCGCCCAG CTAGCTATAA GAAAGCTATA 
GATGAGGATC TGACGGCATT GGCCAAAGAA TTAAGAGAAC TTCGGATTGA AGAAACAAAC 
CGCCCACTGA AGAAGGTAAC AGATTACTCC TCCTCCAGTG AGGAGTCAGA AAGCAGTGAT 
GAAGAGGAGG AAGATGGAGA GAGTGAGACC CAGGATGGGA CGGTGGCTGT CAGCGACATA 
CCCAGACTGA TACCCACAGG AGCCCCCGGG AGCGCTGAAC AGTACAATGT GGGAATGGTC 
GGCACACACG CGCTGGAGAC CTCTCATGCA GATACTTTCG GCAGCAGTAT TTCAAGAGAA 
GGAACCTTGA TGATAAGAGA ATATGGCCTG GGGAGCAGCA CCAAAGCCTC CTTCACCCCC 
TTTGTGGACC CCAGGGTATA CCAGACATCT CCCACTGATG AAGATGAAGA GGATGAGGAA 
TCATCAGCTG CGGGAACCCA GAGGTATCCA GGGGCCCTGG GAAAAGCTGT TCCTCCGAGA 
TCTCCAAACT CACCACCTAT TTCTCAAAAG CAGTTTCTGG CTCTGTTTAC TAGCGAACTT 
CTTAGGCAAG AACAGGCCAA ACTAAATGAA GCAAGAAAGA TTTCAGTGGT AAATGTAAAC 
CCAACCAACA TTCGGCCTCA TAGCGACACA CCGGAGATCA GAAAATATAA GAAGCGATTC 
AATTCAGAAA TACTTTGTGC AGCTCTGTGG GGTGTAAACC TCCTGGTGGG AACCGAAAAT 
GGCCTGATGC TTTTGGACCG AAGTGGGCAA GGCAAAGTTT ATAATCTGAT CAACCGGAGG 
CGATTTCAGC AGATGGATGT GCTCGAGGGA CTGAATGTCC TCGTGACAAT ATCAGGAAAG 
AAGAATAAGT TACGAGTTTA CTATCTTTCA TGGTTAAGAA ACAGAATACT ACACAATGAC 
CCAGAAGTAG AAAAGAAACA AGGCTGGATC ACTGTGGGAG ACTTGGAAGG CTGTATACAT 
TACAAAGTTG TTAAATATGA AAGGATCAAA TTCTTGGTGA TTGCCTTAAA GAATGCTGTG 
GAGATATATG CTTGGGCTCC TAAACCGTAT CATAAGTTCA TGGCATTTAA GTCTTTTGCA 
GATCTCCAGC ACAAGCCACT GCTTGTTGAT CTCACAGTAG AAGAAGGTCA AAGATTAAAG 
GTTATTTTTG GTTCACACAC TGGTTTCCAT GTAATTGATG TTGATTCAGG AAACTCTTAT 
GATATCTACA TACCATCTCA TATTCAGGGC AATATCACTC CTCATGCCAT TGTCATCTTG 
CCTAAGACAG ATGGAATGGA AATGCTTGTT TGCTATGAGG ATGAGGGGGT GTATGTAAAC 
ACCTACGGCC GGATAACTAA GGATGTGGTG CTCCAATGGG GAGAAATGCC CACGTCTGTG 
GCCTACATTC ATTCCAATCA GATAATGGGC TGGGGCGAGA AAGCTATTGA GATCCGGTCA 
GTGGAAACAG GACATTTGGA TGGAGTATTT ATGCATAAGC GAGCTCAAAG GTTAAAGTTT 
CTATGTGAAA GAAATGATAA GGTATTTTTT GCATCCGTGC GATCTGGAGG AAGTAGCCAA 
GTGTTTTTCA TGACCCTCAA CAGAAATTCC ATGATGAACT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TNIK
Entrez Gene ID	539627
Full Name	TRAF2 and NCK interacting kinase
General protein information	Preferred Names TRAF2 and NCK interacting kinase Names TRAF2 and NCK-interacting protein kinase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Bos taurus (cattle)	TNIK	XP_005201764.1
Danio rerio (zebrafish)	tnikb	NP_001032765.2
Drosophila melanogaster (fruit fly)	msn	NP_995971.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TNIK cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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