msn cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following msn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the msn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa19363 NM_001274404.1
Latest version!
Drosophila melanogaster misshapen (msn), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa19363 NM_206252.3
Latest version!
Drosophila melanogaster misshapen (msn), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa19364 NM_001274403.1
Latest version!
Drosophila melanogaster misshapen (msn), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa19365 NM_001274402.1
Latest version!
Drosophila melanogaster misshapen (msn), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OFa20455 NM_206251.3
Latest version!
Drosophila melanogaster misshapen (msn), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OFa20455 NM_206250.3
Latest version!
Drosophila melanogaster misshapen (msn), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OFa20456 NM_079940.5
Latest version!
Drosophila melanogaster misshapen (msn), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OFa20457 NM_206249.3
Latest version!
Drosophila melanogaster misshapen (msn), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa19363
    Clone ID Related Accession (Same CDS sequence) NM_001274404.1 , NM_206252.3
    Accession Version NM_001274404.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misshapen, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGTTAC CACCAAGGAG ACCTGAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA
    TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG
    TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG
    CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC
    AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA
    TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC
    ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG
    CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA
    GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC
    ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG
    GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGTA TCAAGCGGCC
    ATTAGCTCAT CCGTGCATTC CACGCCATCG AAATCGTTTA TCGCCAGCAG CGGAAGCGGT
    GGTCTCGGTT TGGGGGGCGG TACCGTTGTG GGCGGCGTGA TTATCAGCAG CAATCACTCG
    CCCCAATCGA CGATATCGCT CGCCTCTTCG TCCTCGAATT CGCGCCAGAA TTCGCCCAAG
    AATTCGATCA GCAAGACGCG CTCCTCCAGC AGTATTACTA ATCTCTTGCA CAAATCTGCT
    TCTTCCTCCT CCGCGAACCT GCACCACCTG ACGCCCTGCT CCTCGACGTC CTCGGCCTCG
    ATCTCCAATC CGCTGCCACC GCACGCCTAT GCCCTGCAAC AGAAGCAGCG CAGTTTCCTG
    ACCTTTGGCT TCGGCGCCGG CGGCTCTGGT CCTTCGCGCC GGGAATCGCA CGTCAATGTC
    AATGTAACGC CCACCTCCCA CGAGGCGGCC AACGATACGC CCGAGATCCG TAAGTATAAG
    AAGCGCTTCA ATTCGGAAAT CCTGTGCGCA GCGCTATGGG GCGTCAACCT GCTGATTGGA
    ACGGAGAATG GCTTAATGCT GCTAGATCGA TCCGGTCAGG GCAAGGTCTA CCAGCTCATC
    TCGCGACGCC GTTTCCAGCA AATGGAGGTG CTAGAGGGCC AGAACATATT GGTCACCATA
    TCCGGCAAGA AGAACCGAGT GCGCGTCTAC TACTTGTCCT GGCTAAAGTC GAAGATCTTG
    CGCACCGACG GACTCTCCGA TCAAGTGGAG CGTCGGAACG GTTGGATCAA CGTGGGTGAT
    CTGCAAGGTG CTGTACATTT TAAGATTGTC AAATACGAGA GGATTAAGTT CCTAGTGATT
    GCATTAAAAG ACTCTATTGA AATTTATGCA TGGGCTCCCA AACCATATCA CAAATTTATG
    GCATTTAAGA ATTTTGGTGA ACTGGAACAT CGCCCGCTTT TGGTCGATCT CACCATTGAG
    GATCAGTCGA GACTGAAGGT GATCTATGGC TCCGCCGAGG GTTTCCATGC GGTTGATTTA
    GATTCAGCTG AAGTATACGA TATCTATCTT CCCAAGCATA CTCAGGGTGC AATCATTCCG
    CATTGTATTG TGGCGCTTCC CAACTCAAAT GGCATGCAAC TGCTGCTGTG CTACGACAAT
    GAGGGCGTCT ATGTGAACAC TGTGGGCCGC GTTTCCAAGA ACATTGTACT GCAGTGGGGC
    GAGATGCCCA CCTCGGTGGC CTACATCGGC ACTGGACAAA TCATGGGCTG GGGCAATAAA
    GCAATAGAGA TACGTTCCGT TGAGAGCGGC CATTTGGATG GTGTGTTCAT GCACAAAAAG
    GCGCAGCGCT TGAAATTCCT TTGCGAGCGA AACGACAAAG TATTCTTCAG CAGTGCCAAA
    GGTGCTTCAT CGTGTCAAAT CTACTTTATG ACGCTCAACA AGCCGGGCAT GGCCAATTGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261333.1
    CDS332..3934
    Translation

    Target ORF information:

    RefSeq Version NM_001274404.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misshapen (msn), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274404.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGTTAC CACCAAGGAG ACCTGAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA
    TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG
    TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG
    CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC
    AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA
    TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC
    ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG
    CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA
    GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC
    ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG
    GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGTA TCAAGCGGCC
    ATTAGCTCAT CCGTGCATTC CACGCCATCG AAATCGTTTA TCGCCAGCAG CGGAAGCGGT
    GGTCTCGGTT TGGGGGGCGG TACCGTTGTG GGCGGCGTGA TTATCAGCAG CAATCACTCG
    CCCCAATCGA CGATATCGCT CGCCTCTTCG TCCTCGAATT CGCGCCAGAA TTCGCCCAAG
    AATTCGATCA GCAAGACGCG CTCCTCCAGC AGTATTACTA ATCTCTTGCA CAAATCTGCT
    TCTTCCTCCT CCGCGAACCT GCACCACCTG ACGCCCTGCT CCTCGACGTC CTCGGCCTCG
    ATCTCCAATC CGCTGCCACC GCACGCCTAT GCCCTGCAAC AGAAGCAGCG CAGTTTCCTG
    ACCTTTGGCT TCGGCGCCGG CGGCTCTGGT CCTTCGCGCC GGGAATCGCA CGTCAATGTC
    AATGTAACGC CCACCTCCCA CGAGGCGGCC AACGATACGC CCGAGATCCG TAAGTATAAG
    AAGCGCTTCA ATTCGGAAAT CCTGTGCGCA GCGCTATGGG GCGTCAACCT GCTGATTGGA
    ACGGAGAATG GCTTAATGCT GCTAGATCGA TCCGGTCAGG GCAAGGTCTA CCAGCTCATC
    TCGCGACGCC GTTTCCAGCA AATGGAGGTG CTAGAGGGCC AGAACATATT GGTCACCATA
    TCCGGCAAGA AGAACCGAGT GCGCGTCTAC TACTTGTCCT GGCTAAAGTC GAAGATCTTG
    CGCACCGACG GACTCTCCGA TCAAGTGGAG CGTCGGAACG GTTGGATCAA CGTGGGTGAT
    CTGCAAGGTG CTGTACATTT TAAGATTGTC AAATACGAGA GGATTAAGTT CCTAGTGATT
    GCATTAAAAG ACTCTATTGA AATTTATGCA TGGGCTCCCA AACCATATCA CAAATTTATG
    GCATTTAAGA ATTTTGGTGA ACTGGAACAT CGCCCGCTTT TGGTCGATCT CACCATTGAG
    GATCAGTCGA GACTGAAGGT GATCTATGGC TCCGCCGAGG GTTTCCATGC GGTTGATTTA
    GATTCAGCTG AAGTATACGA TATCTATCTT CCCAAGCATA CTCAGGGTGC AATCATTCCG
    CATTGTATTG TGGCGCTTCC CAACTCAAAT GGCATGCAAC TGCTGCTGTG CTACGACAAT
    GAGGGCGTCT ATGTGAACAC TGTGGGCCGC GTTTCCAAGA ACATTGTACT GCAGTGGGGC
    GAGATGCCCA CCTCGGTGGC CTACATCGGC ACTGGACAAA TCATGGGCTG GGGCAATAAA
    GCAATAGAGA TACGTTCCGT TGAGAGCGGC CATTTGGATG GTGTGTTCAT GCACAAAAAG
    GCGCAGCGCT TGAAATTCCT TTGCGAGCGA AACGACAAAG TATTCTTCAG CAGTGCCAAA
    GGTGCTTCAT CGTGTCAAAT CTACTTTATG ACGCTCAACA AGCCGGGCAT GGCCAATTGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa19363
    Clone ID Related Accession (Same CDS sequence) NM_001274404.1 , NM_206252.3
    Accession Version NM_206252.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misshapen, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206252.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGTTAC CACCAAGGAG ACCTGAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA
    TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG
    TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG
    CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC
    AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA
    TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC
    ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG
    CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA
    GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC
    ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG
    GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGTA TCAAGCGGCC
    ATTAGCTCAT CCGTGCATTC CACGCCATCG AAATCGTTTA TCGCCAGCAG CGGAAGCGGT
    GGTCTCGGTT TGGGGGGCGG TACCGTTGTG GGCGGCGTGA TTATCAGCAG CAATCACTCG
    CCCCAATCGA CGATATCGCT CGCCTCTTCG TCCTCGAATT CGCGCCAGAA TTCGCCCAAG
    AATTCGATCA GCAAGACGCG CTCCTCCAGC AGTATTACTA ATCTCTTGCA CAAATCTGCT
    TCTTCCTCCT CCGCGAACCT GCACCACCTG ACGCCCTGCT CCTCGACGTC CTCGGCCTCG
    ATCTCCAATC CGCTGCCACC GCACGCCTAT GCCCTGCAAC AGAAGCAGCG CAGTTTCCTG
    ACCTTTGGCT TCGGCGCCGG CGGCTCTGGT CCTTCGCGCC GGGAATCGCA CGTCAATGTC
    AATGTAACGC CCACCTCCCA CGAGGCGGCC AACGATACGC CCGAGATCCG TAAGTATAAG
    AAGCGCTTCA ATTCGGAAAT CCTGTGCGCA GCGCTATGGG GCGTCAACCT GCTGATTGGA
    ACGGAGAATG GCTTAATGCT GCTAGATCGA TCCGGTCAGG GCAAGGTCTA CCAGCTCATC
    TCGCGACGCC GTTTCCAGCA AATGGAGGTG CTAGAGGGCC AGAACATATT GGTCACCATA
    TCCGGCAAGA AGAACCGAGT GCGCGTCTAC TACTTGTCCT GGCTAAAGTC GAAGATCTTG
    CGCACCGACG GACTCTCCGA TCAAGTGGAG CGTCGGAACG GTTGGATCAA CGTGGGTGAT
    CTGCAAGGTG CTGTACATTT TAAGATTGTC AAATACGAGA GGATTAAGTT CCTAGTGATT
    GCATTAAAAG ACTCTATTGA AATTTATGCA TGGGCTCCCA AACCATATCA CAAATTTATG
    GCATTTAAGA ATTTTGGTGA ACTGGAACAT CGCCCGCTTT TGGTCGATCT CACCATTGAG
    GATCAGTCGA GACTGAAGGT GATCTATGGC TCCGCCGAGG GTTTCCATGC GGTTGATTTA
    GATTCAGCTG AAGTATACGA TATCTATCTT CCCAAGCATA CTCAGGGTGC AATCATTCCG
    CATTGTATTG TGGCGCTTCC CAACTCAAAT GGCATGCAAC TGCTGCTGTG CTACGACAAT
    GAGGGCGTCT ATGTGAACAC TGTGGGCCGC GTTTCCAAGA ACATTGTACT GCAGTGGGGC
    GAGATGCCCA CCTCGGTGGC CTACATCGGC ACTGGACAAA TCATGGGCTG GGGCAATAAA
    GCAATAGAGA TACGTTCCGT TGAGAGCGGC CATTTGGATG GTGTGTTCAT GCACAAAAAG
    GCGCAGCGCT TGAAATTCCT TTGCGAGCGA AACGACAAAG TATTCTTCAG CAGTGCCAAA
    GGTGCTTCAT CGTGTCAAAT CTACTTTATG ACGCTCAACA AGCCGGGCAT GGCCAATTGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_995974.1
    CDS195..3797
    Translation

    Target ORF information:

    RefSeq Version NM_206252.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misshapen (msn), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206252.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGTTAC CACCAAGGAG ACCTGAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA
    TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG
    TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG
    CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC
    AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA
    TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC
    ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG
    CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA
    GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC
    ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG
    GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGTA TCAAGCGGCC
    ATTAGCTCAT CCGTGCATTC CACGCCATCG AAATCGTTTA TCGCCAGCAG CGGAAGCGGT
    GGTCTCGGTT TGGGGGGCGG TACCGTTGTG GGCGGCGTGA TTATCAGCAG CAATCACTCG
    CCCCAATCGA CGATATCGCT CGCCTCTTCG TCCTCGAATT CGCGCCAGAA TTCGCCCAAG
    AATTCGATCA GCAAGACGCG CTCCTCCAGC AGTATTACTA ATCTCTTGCA CAAATCTGCT
    TCTTCCTCCT CCGCGAACCT GCACCACCTG ACGCCCTGCT CCTCGACGTC CTCGGCCTCG
    ATCTCCAATC CGCTGCCACC GCACGCCTAT GCCCTGCAAC AGAAGCAGCG CAGTTTCCTG
    ACCTTTGGCT TCGGCGCCGG CGGCTCTGGT CCTTCGCGCC GGGAATCGCA CGTCAATGTC
    AATGTAACGC CCACCTCCCA CGAGGCGGCC AACGATACGC CCGAGATCCG TAAGTATAAG
    AAGCGCTTCA ATTCGGAAAT CCTGTGCGCA GCGCTATGGG GCGTCAACCT GCTGATTGGA
    ACGGAGAATG GCTTAATGCT GCTAGATCGA TCCGGTCAGG GCAAGGTCTA CCAGCTCATC
    TCGCGACGCC GTTTCCAGCA AATGGAGGTG CTAGAGGGCC AGAACATATT GGTCACCATA
    TCCGGCAAGA AGAACCGAGT GCGCGTCTAC TACTTGTCCT GGCTAAAGTC GAAGATCTTG
    CGCACCGACG GACTCTCCGA TCAAGTGGAG CGTCGGAACG GTTGGATCAA CGTGGGTGAT
    CTGCAAGGTG CTGTACATTT TAAGATTGTC AAATACGAGA GGATTAAGTT CCTAGTGATT
    GCATTAAAAG ACTCTATTGA AATTTATGCA TGGGCTCCCA AACCATATCA CAAATTTATG
    GCATTTAAGA ATTTTGGTGA ACTGGAACAT CGCCCGCTTT TGGTCGATCT CACCATTGAG
    GATCAGTCGA GACTGAAGGT GATCTATGGC TCCGCCGAGG GTTTCCATGC GGTTGATTTA
    GATTCAGCTG AAGTATACGA TATCTATCTT CCCAAGCATA CTCAGGGTGC AATCATTCCG
    CATTGTATTG TGGCGCTTCC CAACTCAAAT GGCATGCAAC TGCTGCTGTG CTACGACAAT
    GAGGGCGTCT ATGTGAACAC TGTGGGCCGC GTTTCCAAGA ACATTGTACT GCAGTGGGGC
    GAGATGCCCA CCTCGGTGGC CTACATCGGC ACTGGACAAA TCATGGGCTG GGGCAATAAA
    GCAATAGAGA TACGTTCCGT TGAGAGCGGC CATTTGGATG GTGTGTTCAT GCACAAAAAG
    GCGCAGCGCT TGAAATTCCT TTGCGAGCGA AACGACAAAG TATTCTTCAG CAGTGCCAAA
    GGTGCTTCAT CGTGTCAAAT CTACTTTATG ACGCTCAACA AGCCGGGCAT GGCCAATTGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa19364
    Clone ID Related Accession (Same CDS sequence) NM_001274403.1
    Accession Version NM_001274403.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misshapen, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGGATC ATTTGGATGT GTTAGCAGCA CAATTAAGTG AATTGGGCGT GGTCTTCTCC
    CAACAGCCAC AGCCGCAGAC GGCCGCCGGT GGTCAGGGAT CCCAGCAGCA GGCGCAGCCG
    GAGGCGCCGC CTCGCAACAA TCGCCAGTCG AGCGGTCTGT CCTCCTCCGG CGGATCGGCA
    TCCGGAGGCG GCGGTTCGTC GAAGCCCGCA GCCGCTCTGC CGCAGCAGTC GAACAATCAT
    TTGGGCCAGC CGGTGAATCC GCTGGATCCC TTGGACAGCA GCGATTCGGA CAGCGAGCCG
    GATGAGCCCA ACGATCGGGC TAGGAACGAT GGAACTCTAT TGGCCAGTGA TCCGCCCAAG
    CCACTGCCCG GCTTGGGACC CGTCTCCGAG GACGCCAACA CGACAACGCC CTTGAGTCAC
    GGCAGCGGTG GACCGCCCAA CCGCCCACTG CCGCCCACGC CCGACGACGA CGATCAGGCT
    GGCGATCGGA CTTTGATCAT GAAGCGCAAT CGAGGAGGAG GCGGTGGCGG CGGCGGCGGC
    GGAGGAGGCG GTGGCAGCAG CGGCGTGGGC GCCGATGGCA CTGGACTGGG AACTCCTGGG
    ACAAGGACCA GCAGCGTCCT GCCGGATCTA CTCAGCCAGG CATCGCCGGC AACGCCACCA
    CGCCATGATA AATCATCCAG TGAGGAGTAT CAAGCGGCCA TTAGCTCATC CGTGCATTCC
    ACGCCATCGA AATCGTTTAT CGCCAGCAGC GGAAGCGGTG GTCTCGGTTT GGGGGGCGGT
    ACCGTTGTGG GCGGCGTGAT TATCAGCAGC AATCACTCGC CCCAATCGAC GATATCGCTC
    GCCTCTTCGT CCTCGAATTC GCGCCAGAAT TCGCCCAAGA ATTCGATCAG CAAGACGCGC
    TCCTCCAGCA GTATTACTAA TCTCTTGCAC AAATCTGCTT CTTCCTCCTC CGCGAACCTG
    CACCACCTGA CGCCCTGCTC CTCGACGTCC TCGGCCTCGA TCTCCAATCC GCTGCCACCG
    CACGCCTATG CCCTGCAACA GAAGCAGCGC AGTTTCCTGA CCTTTGGCTT CGGCGCCGGC
    GGCTCTGGTC CTTCGCGCCG GGAATCGCAC GTCAATGTCA ATGTAACGCC CACCTCCCAC
    GAGGCGGCCA ACGATACGCC CGAGATCCGT AAGTATAAGA AGCGCTTCAA TTCGGAAATC
    CTGTGCGCAG CGCTATGGGG CGTCAACCTG CTGATTGGAA CGGAGAATGG CTTAATGCTG
    CTAGATCGAT CCGGTCAGGG CAAGGTCTAC CAGCTCATCT CGCGACGCCG TTTCCAGCAA
    ATGGAGGTGC TAGAGGGCCA GAACATATTG GTCACCATAT CCGGCAAGAA GAACCGAGTG
    CGCGTCTACT ACTTGTCCTG GCTAAAGTCG AAGATCTTGC GCACCGACGG ACTCTCCGAT
    CAAGTGGAGC GTCGGAACGG TTGGATCAAC GTGGGTGATC TGCAAGGTGC TGTACATTTT
    AAGATTGTCA AATACGAGAG GATTAAGTTC CTAGTGATTG CATTAAAAGA CTCTATTGAA
    ATTTATGCAT GGGCTCCCAA ACCATATCAC AAATTTATGG CATTTAAGAA TTTTGGTGAA
    CTGGAACATC GCCCGCTTTT GGTCGATCTC ACCATTGAGG ATCAGTCGAG ACTGAAGGTG
    ATCTATGGCT CCGCCGAGGG TTTCCATGCG GTTGATTTAG ATTCAGCTGA AGTATACGAT
    ATCTATCTTC CCAAGCATAC TCAGGGTGCA ATCATTCCGC ATTGTATTGT GGCGCTTCCC
    AACTCAAATG GCATGCAACT GCTGCTGTGC TACGACAATG AGGGCGTCTA TGTGAACACT
    GTGGGCCGCG TTTCCAAGAA CATTGTACTG CAGTGGGGCG AGATGCCCAC CTCGGTGGCC
    TACATCGGCA CTGGACAAAT CATGGGCTGG GGCAATAAAG CAATAGAGAT ACGTTCCGTT
    GAGAGCGGCC ATTTGGATGG TGTGTTCATG CACAAAAAGG CGCAGCGCTT GAAATTCCTT
    TGCGAGCGAA ACGACAAAGT ATTCTTCAGC AGTGCCAAAG GTGCTTCATC GTGTCAAATC
    TACTTTATGA CGCTCAACAA GCCGGGCATG GCCAATTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261332.1
    CDS195..3836
    Translation

    Target ORF information:

    RefSeq Version NM_001274403.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misshapen (msn), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274403.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGGATC ATTTGGATGT GTTAGCAGCA CAATTAAGTG AATTGGGCGT GGTCTTCTCC
    CAACAGCCAC AGCCGCAGAC GGCCGCCGGT GGTCAGGGAT CCCAGCAGCA GGCGCAGCCG
    GAGGCGCCGC CTCGCAACAA TCGCCAGTCG AGCGGTCTGT CCTCCTCCGG CGGATCGGCA
    TCCGGAGGCG GCGGTTCGTC GAAGCCCGCA GCCGCTCTGC CGCAGCAGTC GAACAATCAT
    TTGGGCCAGC CGGTGAATCC GCTGGATCCC TTGGACAGCA GCGATTCGGA CAGCGAGCCG
    GATGAGCCCA ACGATCGGGC TAGGAACGAT GGAACTCTAT TGGCCAGTGA TCCGCCCAAG
    CCACTGCCCG GCTTGGGACC CGTCTCCGAG GACGCCAACA CGACAACGCC CTTGAGTCAC
    GGCAGCGGTG GACCGCCCAA CCGCCCACTG CCGCCCACGC CCGACGACGA CGATCAGGCT
    GGCGATCGGA CTTTGATCAT GAAGCGCAAT CGAGGAGGAG GCGGTGGCGG CGGCGGCGGC
    GGAGGAGGCG GTGGCAGCAG CGGCGTGGGC GCCGATGGCA CTGGACTGGG AACTCCTGGG
    ACAAGGACCA GCAGCGTCCT GCCGGATCTA CTCAGCCAGG CATCGCCGGC AACGCCACCA
    CGCCATGATA AATCATCCAG TGAGGAGTAT CAAGCGGCCA TTAGCTCATC CGTGCATTCC
    ACGCCATCGA AATCGTTTAT CGCCAGCAGC GGAAGCGGTG GTCTCGGTTT GGGGGGCGGT
    ACCGTTGTGG GCGGCGTGAT TATCAGCAGC AATCACTCGC CCCAATCGAC GATATCGCTC
    GCCTCTTCGT CCTCGAATTC GCGCCAGAAT TCGCCCAAGA ATTCGATCAG CAAGACGCGC
    TCCTCCAGCA GTATTACTAA TCTCTTGCAC AAATCTGCTT CTTCCTCCTC CGCGAACCTG
    CACCACCTGA CGCCCTGCTC CTCGACGTCC TCGGCCTCGA TCTCCAATCC GCTGCCACCG
    CACGCCTATG CCCTGCAACA GAAGCAGCGC AGTTTCCTGA CCTTTGGCTT CGGCGCCGGC
    GGCTCTGGTC CTTCGCGCCG GGAATCGCAC GTCAATGTCA ATGTAACGCC CACCTCCCAC
    GAGGCGGCCA ACGATACGCC CGAGATCCGT AAGTATAAGA AGCGCTTCAA TTCGGAAATC
    CTGTGCGCAG CGCTATGGGG CGTCAACCTG CTGATTGGAA CGGAGAATGG CTTAATGCTG
    CTAGATCGAT CCGGTCAGGG CAAGGTCTAC CAGCTCATCT CGCGACGCCG TTTCCAGCAA
    ATGGAGGTGC TAGAGGGCCA GAACATATTG GTCACCATAT CCGGCAAGAA GAACCGAGTG
    CGCGTCTACT ACTTGTCCTG GCTAAAGTCG AAGATCTTGC GCACCGACGG ACTCTCCGAT
    CAAGTGGAGC GTCGGAACGG TTGGATCAAC GTGGGTGATC TGCAAGGTGC TGTACATTTT
    AAGATTGTCA AATACGAGAG GATTAAGTTC CTAGTGATTG CATTAAAAGA CTCTATTGAA
    ATTTATGCAT GGGCTCCCAA ACCATATCAC AAATTTATGG CATTTAAGAA TTTTGGTGAA
    CTGGAACATC GCCCGCTTTT GGTCGATCTC ACCATTGAGG ATCAGTCGAG ACTGAAGGTG
    ATCTATGGCT CCGCCGAGGG TTTCCATGCG GTTGATTTAG ATTCAGCTGA AGTATACGAT
    ATCTATCTTC CCAAGCATAC TCAGGGTGCA ATCATTCCGC ATTGTATTGT GGCGCTTCCC
    AACTCAAATG GCATGCAACT GCTGCTGTGC TACGACAATG AGGGCGTCTA TGTGAACACT
    GTGGGCCGCG TTTCCAAGAA CATTGTACTG CAGTGGGGCG AGATGCCCAC CTCGGTGGCC
    TACATCGGCA CTGGACAAAT CATGGGCTGG GGCAATAAAG CAATAGAGAT ACGTTCCGTT
    GAGAGCGGCC ATTTGGATGG TGTGTTCATG CACAAAAAGG CGCAGCGCTT GAAATTCCTT
    TGCGAGCGAA ACGACAAAGT ATTCTTCAGC AGTGCCAAAG GTGCTTCATC GTGTCAAATC
    TACTTTATGA CGCTCAACAA GCCGGGCATG GCCAATTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa19365
    Clone ID Related Accession (Same CDS sequence) NM_001274402.1
    Accession Version NM_001274402.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misshapen, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT
    GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA
    TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG
    TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG
    CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC
    AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA
    TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC
    ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG
    CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA ACTAGAGCAG
    AACATAAACC GCCTGCAAAA GTCCGCATCC ACATCGCAAG CCAATGTGAC ACCGTCGCGT
    CGCGGCGACG AATCCAATTT GCTGCGGGAC TGGGACTTTG ATCGTTTCTT CCCCAAGAAC
    GCAAACGGTC CGAGGGGCAG TGGACGTGGT AGCCCCACAA CCACTGCCAG CTTGAGCCGA
    AGCTCCCAGC TGAGCACGCT GAAGGTGGAT ACGAAACTGC AAAGGGCCAG TGTGGCGGAG
    GCCATCACGA GACCAGTTCC CCGGGGCTAT CAGCCGCTAA AGGCCGAACC AAGTGCCAGC
    CAAAGTATTG CCAAAGAACA AGGATCAGCA TCAGGATCCG GATCGGGATC GGGAAGTGCT
    AGTGGTAGCG GAAGCGGTGG TAGCACCAAT TCCCCAGCGC ATAAGCGCCA GGATTCGGAC
    TCCCGATTGC CAACGAATTT TGAGCGCGGT TTTCGACGCG AGAACTCGGA CTTCTTCCCG
    CTGGCCAAGA GATACTCGGC TGTGTTCAGT GGTGCAAGTG CATCCGGATC AACAGCTGGA
    TCGCCGTCAG CACAGGCTCT TCAGAGGTCG AGCGCAGTGT ACCAAAGGAA CAGCATTTAT
    AGCAGCAGCA TCAGCAGTAA GAGCAAGGAG AATGACGTGC CTGGAGCGCC AGGAGCAGCT
    GCAGCAGGAA CTGCCACTGC CAGTGCCAAG CCAGGACCAG CTGCAGCCAC AACCACTGCC
    ACCAAGGGAG CTGCTCCAAA GACCTCCAAG TCCCTGGCCA ACTTTCATTT CCTGCGACCG
    CGTCGCGAAA AGACTGAATC CGTCATTGTG CTGCAGAATG CTGCCGTTCG CGCCCAAAGG
    CTGCAGCAAC TGCAACAGCA GCAGCAGCAG CAGCAACTGC AGCAGCAACA GCAGCAGCAG
    AATCGAGGAG GAGGCGGTGG CGGCGGCGGC GGCGGAGGAG GCGGTGGCAG CAGCGGCGTG
    GGCGCCGATG GCACTGGACT GGGAACTCCT GGGACAAGGA CCAGCAGCGT CCTGCCGGAT
    CTACTCAGCC AGGCATCGCC GGCAACGCCA CCACGCCATG ATAAATCATC CAGTGAGGAG
    AAGCAGCGCA GTTTCCTGAC CTTTGGCTTC GGCGCCGGCG GCTCTGGTCC TTCGCGCCGG
    GAATCGCACG TCAATGTCAA TGTAACGCCC ACCTCCCACG AGGCGGCCAA CGATACGCCC
    GAGATCCGTA AGTATAAGAA GCGCTTCAAT TCGGAAATCC TGTGCGCAGC GCTATGGGGC
    GTCAACCTGC TGATTGGAAC GGAGAATGGC TTAATGCTGC TAGATCGATC CGGTCAGGGC
    AAGGTCTACC AGCTCATCTC GCGACGCCGT TTCCAGCAAA TGGAGGTGCT AGAGGGCCAG
    AACATATTGG TCACCATATC CGGCAAGAAG AACCGAGTGC GCGTCTACTA CTTGTCCTGG
    CTAAAGTCGA AGATCTTGCG CACCGACGGA CTCTCCGATC AAGTGGAGCG TCGGAACGGT
    TGGATCAACG TGGGTGATCT GCAAGGTGCT GTACATTTTA AGATTGTCAA ATACGAGAGG
    ATTAAGTTCC TAGTGATTGC ATTAAAAGAC TCTATTGAAA TTTATGCATG GGCTCCCAAA
    CCATATCACA AATTTATGGC ATTTAAGAAT TTTGGTGAAC TGGAACATCG CCCGCTTTTG
    GTCGATCTCA CCATTGAGGA TCAGTCGAGA CTGAAGGTGA TCTATGGCTC CGCCGAGGGT
    TTCCATGCGG TTGATTTAGA TTCAGCTGAA GTATACGATA TCTATCTTCC CAAGCATGGT
    GCAATCATTC CGCATTGTAT TGTGGCGCTT CCCAACTCAA ATGGCATGCA ACTGCTGCTG
    TGCTACGACA ATGAGGGCGT CTATGTGAAC ACTGTGGGCC GCGTTTCCAA GAACATTGTA
    CTGCAGTGGG GCGAGATGCC CACCTCGGTG GCCTACATCG GCACTGGACA AATCATGGGC
    TGGGGCAATA AAGCAATAGA GATACGTTCC GTTGAGAGCG GCCATTTGGA TGGTGTGTTC
    ATGCACAAAA AGGCGCAGCG CTTGAAATTC CTTTGCGAGC GAAACGACAA AGTATTCTTC
    AGCAGTGCCA AAGGTGCTTC ATCGTGTCAA ATCTACTTTA TGACGCTCAA CAAGCCGGGC
    ATGGCCAATT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261331.1
    CDS275..4489
    Translation

    Target ORF information:

    RefSeq Version NM_001274402.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misshapen (msn), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274402.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT
    GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA
    TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG
    TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG
    CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC
    AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA
    TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC
    ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG
    CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA ACTAGAGCAG
    AACATAAACC GCCTGCAAAA GTCCGCATCC ACATCGCAAG CCAATGTGAC ACCGTCGCGT
    CGCGGCGACG AATCCAATTT GCTGCGGGAC TGGGACTTTG ATCGTTTCTT CCCCAAGAAC
    GCAAACGGTC CGAGGGGCAG TGGACGTGGT AGCCCCACAA CCACTGCCAG CTTGAGCCGA
    AGCTCCCAGC TGAGCACGCT GAAGGTGGAT ACGAAACTGC AAAGGGCCAG TGTGGCGGAG
    GCCATCACGA GACCAGTTCC CCGGGGCTAT CAGCCGCTAA AGGCCGAACC AAGTGCCAGC
    CAAAGTATTG CCAAAGAACA AGGATCAGCA TCAGGATCCG GATCGGGATC GGGAAGTGCT
    AGTGGTAGCG GAAGCGGTGG TAGCACCAAT TCCCCAGCGC ATAAGCGCCA GGATTCGGAC
    TCCCGATTGC CAACGAATTT TGAGCGCGGT TTTCGACGCG AGAACTCGGA CTTCTTCCCG
    CTGGCCAAGA GATACTCGGC TGTGTTCAGT GGTGCAAGTG CATCCGGATC AACAGCTGGA
    TCGCCGTCAG CACAGGCTCT TCAGAGGTCG AGCGCAGTGT ACCAAAGGAA CAGCATTTAT
    AGCAGCAGCA TCAGCAGTAA GAGCAAGGAG AATGACGTGC CTGGAGCGCC AGGAGCAGCT
    GCAGCAGGAA CTGCCACTGC CAGTGCCAAG CCAGGACCAG CTGCAGCCAC AACCACTGCC
    ACCAAGGGAG CTGCTCCAAA GACCTCCAAG TCCCTGGCCA ACTTTCATTT CCTGCGACCG
    CGTCGCGAAA AGACTGAATC CGTCATTGTG CTGCAGAATG CTGCCGTTCG CGCCCAAAGG
    CTGCAGCAAC TGCAACAGCA GCAGCAGCAG CAGCAACTGC AGCAGCAACA GCAGCAGCAG
    AATCGAGGAG GAGGCGGTGG CGGCGGCGGC GGCGGAGGAG GCGGTGGCAG CAGCGGCGTG
    GGCGCCGATG GCACTGGACT GGGAACTCCT GGGACAAGGA CCAGCAGCGT CCTGCCGGAT
    CTACTCAGCC AGGCATCGCC GGCAACGCCA CCACGCCATG ATAAATCATC CAGTGAGGAG
    AAGCAGCGCA GTTTCCTGAC CTTTGGCTTC GGCGCCGGCG GCTCTGGTCC TTCGCGCCGG
    GAATCGCACG TCAATGTCAA TGTAACGCCC ACCTCCCACG AGGCGGCCAA CGATACGCCC
    GAGATCCGTA AGTATAAGAA GCGCTTCAAT TCGGAAATCC TGTGCGCAGC GCTATGGGGC
    GTCAACCTGC TGATTGGAAC GGAGAATGGC TTAATGCTGC TAGATCGATC CGGTCAGGGC
    AAGGTCTACC AGCTCATCTC GCGACGCCGT TTCCAGCAAA TGGAGGTGCT AGAGGGCCAG
    AACATATTGG TCACCATATC CGGCAAGAAG AACCGAGTGC GCGTCTACTA CTTGTCCTGG
    CTAAAGTCGA AGATCTTGCG CACCGACGGA CTCTCCGATC AAGTGGAGCG TCGGAACGGT
    TGGATCAACG TGGGTGATCT GCAAGGTGCT GTACATTTTA AGATTGTCAA ATACGAGAGG
    ATTAAGTTCC TAGTGATTGC ATTAAAAGAC TCTATTGAAA TTTATGCATG GGCTCCCAAA
    CCATATCACA AATTTATGGC ATTTAAGAAT TTTGGTGAAC TGGAACATCG CCCGCTTTTG
    GTCGATCTCA CCATTGAGGA TCAGTCGAGA CTGAAGGTGA TCTATGGCTC CGCCGAGGGT
    TTCCATGCGG TTGATTTAGA TTCAGCTGAA GTATACGATA TCTATCTTCC CAAGCATGGT
    GCAATCATTC CGCATTGTAT TGTGGCGCTT CCCAACTCAA ATGGCATGCA ACTGCTGCTG
    TGCTACGACA ATGAGGGCGT CTATGTGAAC ACTGTGGGCC GCGTTTCCAA GAACATTGTA
    CTGCAGTGGG GCGAGATGCC CACCTCGGTG GCCTACATCG GCACTGGACA AATCATGGGC
    TGGGGCAATA AAGCAATAGA GATACGTTCC GTTGAGAGCG GCCATTTGGA TGGTGTGTTC
    ATGCACAAAA AGGCGCAGCG CTTGAAATTC CTTTGCGAGC GAAACGACAA AGTATTCTTC
    AGCAGTGCCA AAGGTGCTTC ATCGTGTCAA ATCTACTTTA TGACGCTCAA CAAGCCGGGC
    ATGGCCAATT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20455
    Clone ID Related Accession (Same CDS sequence) NM_206251.3 , NM_206250.3
    Accession Version NM_206251.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3309bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misshapen, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206251.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT
    GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA
    TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG
    TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG
    CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC
    AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA
    TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC
    ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG
    CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA
    GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC
    ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG
    GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGAA GCAGCGCAGT
    TTCCTGACCT TTGGCTTCGG CGCCGGCGGC TCTGGTCCTT CGCGCCGGGA ATCGCACGTC
    AATGTCAATG TAACGCCCAC CTCCCACGAG GCGGCCAACG ATACGCCCGA GATCCGTAAG
    TATAAGAAGC GCTTCAATTC GGAAATCCTG TGCGCAGCGC TATGGGGCGT CAACCTGCTG
    ATTGGAACGG AGAATGGCTT AATGCTGCTA GATCGATCCG GTCAGGGCAA GGTCTACCAG
    CTCATCTCGC GACGCCGTTT CCAGCAAATG GAGGTGCTAG AGGGCCAGAA CATATTGGTC
    ACCATATCCG GCAAGAAGAA CCGAGTGCGC GTCTACTACT TGTCCTGGCT AAAGTCGAAG
    ATCTTGCGCA CCGACGGACT CTCCGATCAA GTGGAGCGTC GGAACGGTTG GATCAACGTG
    GGTGATCTGC AAGGTGCTGT ACATTTTAAG ATTGTCAAAT ACGAGAGGAT TAAGTTCCTA
    GTGATTGCAT TAAAAGACTC TATTGAAATT TATGCATGGG CTCCCAAACC ATATCACAAA
    TTTATGGCAT TTAAGAATTT TGGTGAACTG GAACATCGCC CGCTTTTGGT CGATCTCACC
    ATTGAGGATC AGTCGAGACT GAAGGTGATC TATGGCTCCG CCGAGGGTTT CCATGCGGTT
    GATTTAGATT CAGCTGAAGT ATACGATATC TATCTTCCCA AGCATACTCA GGGTGCAATC
    ATTCCGCATT GTATTGTGGC GCTTCCCAAC TCAAATGGCA TGCAACTGCT GCTGTGCTAC
    GACAATGAGG GCGTCTATGT GAACACTGTG GGCCGCGTTT CCAAGAACAT TGTACTGCAG
    TGGGGCGAGA TGCCCACCTC GGTGGCCTAC ATCGGCACTG GACAAATCAT GGGCTGGGGC
    AATAAAGCAA TAGAGATACG TTCCGTTGAG AGCGGCCATT TGGATGGTGT GTTCATGCAC
    AAAAAGGCGC AGCGCTTGAA ATTCCTTTGC GAGCGAAACG ACAAAGTATT CTTCAGCAGT
    GCCAAAGGTG CTTCATCGTG TCAAATCTAC TTTATGACGC TCAACAAGCC GGGCATGGCC
    AATTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_995973.1
    CDS189..3497
    Translation

    Target ORF information:

    RefSeq Version NM_206251.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misshapen (msn), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206251.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT
    GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA
    TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG
    TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG
    CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC
    AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA
    TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC
    ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG
    CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA
    GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC
    ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG
    GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGAA GCAGCGCAGT
    TTCCTGACCT TTGGCTTCGG CGCCGGCGGC TCTGGTCCTT CGCGCCGGGA ATCGCACGTC
    AATGTCAATG TAACGCCCAC CTCCCACGAG GCGGCCAACG ATACGCCCGA GATCCGTAAG
    TATAAGAAGC GCTTCAATTC GGAAATCCTG TGCGCAGCGC TATGGGGCGT CAACCTGCTG
    ATTGGAACGG AGAATGGCTT AATGCTGCTA GATCGATCCG GTCAGGGCAA GGTCTACCAG
    CTCATCTCGC GACGCCGTTT CCAGCAAATG GAGGTGCTAG AGGGCCAGAA CATATTGGTC
    ACCATATCCG GCAAGAAGAA CCGAGTGCGC GTCTACTACT TGTCCTGGCT AAAGTCGAAG
    ATCTTGCGCA CCGACGGACT CTCCGATCAA GTGGAGCGTC GGAACGGTTG GATCAACGTG
    GGTGATCTGC AAGGTGCTGT ACATTTTAAG ATTGTCAAAT ACGAGAGGAT TAAGTTCCTA
    GTGATTGCAT TAAAAGACTC TATTGAAATT TATGCATGGG CTCCCAAACC ATATCACAAA
    TTTATGGCAT TTAAGAATTT TGGTGAACTG GAACATCGCC CGCTTTTGGT CGATCTCACC
    ATTGAGGATC AGTCGAGACT GAAGGTGATC TATGGCTCCG CCGAGGGTTT CCATGCGGTT
    GATTTAGATT CAGCTGAAGT ATACGATATC TATCTTCCCA AGCATACTCA GGGTGCAATC
    ATTCCGCATT GTATTGTGGC GCTTCCCAAC TCAAATGGCA TGCAACTGCT GCTGTGCTAC
    GACAATGAGG GCGTCTATGT GAACACTGTG GGCCGCGTTT CCAAGAACAT TGTACTGCAG
    TGGGGCGAGA TGCCCACCTC GGTGGCCTAC ATCGGCACTG GACAAATCAT GGGCTGGGGC
    AATAAAGCAA TAGAGATACG TTCCGTTGAG AGCGGCCATT TGGATGGTGT GTTCATGCAC
    AAAAAGGCGC AGCGCTTGAA ATTCCTTTGC GAGCGAAACG ACAAAGTATT CTTCAGCAGT
    GCCAAAGGTG CTTCATCGTG TCAAATCTAC TTTATGACGC TCAACAAGCC GGGCATGGCC
    AATTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20455
    Clone ID Related Accession (Same CDS sequence) NM_206251.3 , NM_206250.3
    Accession Version NM_206250.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3309bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misshapen, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206250.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT
    GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA
    TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG
    TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG
    CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC
    AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA
    TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC
    ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG
    CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA
    GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC
    ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG
    GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGAA GCAGCGCAGT
    TTCCTGACCT TTGGCTTCGG CGCCGGCGGC TCTGGTCCTT CGCGCCGGGA ATCGCACGTC
    AATGTCAATG TAACGCCCAC CTCCCACGAG GCGGCCAACG ATACGCCCGA GATCCGTAAG
    TATAAGAAGC GCTTCAATTC GGAAATCCTG TGCGCAGCGC TATGGGGCGT CAACCTGCTG
    ATTGGAACGG AGAATGGCTT AATGCTGCTA GATCGATCCG GTCAGGGCAA GGTCTACCAG
    CTCATCTCGC GACGCCGTTT CCAGCAAATG GAGGTGCTAG AGGGCCAGAA CATATTGGTC
    ACCATATCCG GCAAGAAGAA CCGAGTGCGC GTCTACTACT TGTCCTGGCT AAAGTCGAAG
    ATCTTGCGCA CCGACGGACT CTCCGATCAA GTGGAGCGTC GGAACGGTTG GATCAACGTG
    GGTGATCTGC AAGGTGCTGT ACATTTTAAG ATTGTCAAAT ACGAGAGGAT TAAGTTCCTA
    GTGATTGCAT TAAAAGACTC TATTGAAATT TATGCATGGG CTCCCAAACC ATATCACAAA
    TTTATGGCAT TTAAGAATTT TGGTGAACTG GAACATCGCC CGCTTTTGGT CGATCTCACC
    ATTGAGGATC AGTCGAGACT GAAGGTGATC TATGGCTCCG CCGAGGGTTT CCATGCGGTT
    GATTTAGATT CAGCTGAAGT ATACGATATC TATCTTCCCA AGCATACTCA GGGTGCAATC
    ATTCCGCATT GTATTGTGGC GCTTCCCAAC TCAAATGGCA TGCAACTGCT GCTGTGCTAC
    GACAATGAGG GCGTCTATGT GAACACTGTG GGCCGCGTTT CCAAGAACAT TGTACTGCAG
    TGGGGCGAGA TGCCCACCTC GGTGGCCTAC ATCGGCACTG GACAAATCAT GGGCTGGGGC
    AATAAAGCAA TAGAGATACG TTCCGTTGAG AGCGGCCATT TGGATGGTGT GTTCATGCAC
    AAAAAGGCGC AGCGCTTGAA ATTCCTTTGC GAGCGAAACG ACAAAGTATT CTTCAGCAGT
    GCCAAAGGTG CTTCATCGTG TCAAATCTAC TTTATGACGC TCAACAAGCC GGGCATGGCC
    AATTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_995972.1
    CDS178..3486
    Translation

    Target ORF information:

    RefSeq Version NM_206250.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misshapen (msn), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206250.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT
    GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA
    TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG
    TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG
    CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC
    AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA
    TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC
    ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG
    CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA
    GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC
    ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG
    GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGAA GCAGCGCAGT
    TTCCTGACCT TTGGCTTCGG CGCCGGCGGC TCTGGTCCTT CGCGCCGGGA ATCGCACGTC
    AATGTCAATG TAACGCCCAC CTCCCACGAG GCGGCCAACG ATACGCCCGA GATCCGTAAG
    TATAAGAAGC GCTTCAATTC GGAAATCCTG TGCGCAGCGC TATGGGGCGT CAACCTGCTG
    ATTGGAACGG AGAATGGCTT AATGCTGCTA GATCGATCCG GTCAGGGCAA GGTCTACCAG
    CTCATCTCGC GACGCCGTTT CCAGCAAATG GAGGTGCTAG AGGGCCAGAA CATATTGGTC
    ACCATATCCG GCAAGAAGAA CCGAGTGCGC GTCTACTACT TGTCCTGGCT AAAGTCGAAG
    ATCTTGCGCA CCGACGGACT CTCCGATCAA GTGGAGCGTC GGAACGGTTG GATCAACGTG
    GGTGATCTGC AAGGTGCTGT ACATTTTAAG ATTGTCAAAT ACGAGAGGAT TAAGTTCCTA
    GTGATTGCAT TAAAAGACTC TATTGAAATT TATGCATGGG CTCCCAAACC ATATCACAAA
    TTTATGGCAT TTAAGAATTT TGGTGAACTG GAACATCGCC CGCTTTTGGT CGATCTCACC
    ATTGAGGATC AGTCGAGACT GAAGGTGATC TATGGCTCCG CCGAGGGTTT CCATGCGGTT
    GATTTAGATT CAGCTGAAGT ATACGATATC TATCTTCCCA AGCATACTCA GGGTGCAATC
    ATTCCGCATT GTATTGTGGC GCTTCCCAAC TCAAATGGCA TGCAACTGCT GCTGTGCTAC
    GACAATGAGG GCGTCTATGT GAACACTGTG GGCCGCGTTT CCAAGAACAT TGTACTGCAG
    TGGGGCGAGA TGCCCACCTC GGTGGCCTAC ATCGGCACTG GACAAATCAT GGGCTGGGGC
    AATAAAGCAA TAGAGATACG TTCCGTTGAG AGCGGCCATT TGGATGGTGT GTTCATGCAC
    AAAAAGGCGC AGCGCTTGAA ATTCCTTTGC GAGCGAAACG ACAAAGTATT CTTCAGCAGT
    GCCAAAGGTG CTTCATCGTG TCAAATCTAC TTTATGACGC TCAACAAGCC GGGCATGGCC
    AATTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20456
    Clone ID Related Accession (Same CDS sequence) NM_079940.5
    Accession Version NM_079940.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4515bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misshapen, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079940.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGTTAC CACCAAGGAG ACCTGAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA
    TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG
    TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG
    CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC
    AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA
    TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC
    ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG
    CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA ACTAGAGCAG
    AACATAAACC GCCTGCAAAA GTCCGCATCC ACATCGCAAG CCAATGTGAC ACCGTCGCGT
    CGCGGCGACG AATCCAATTT GCTGCGGGAC TGGGACTTTG ATCGTTTCTT CCCCAAGAAC
    GCAAACGGTC CGAGGGGCAG TGGACGTGGT AGCCCCACAA CCACTGCCAG CTTGAGCCGA
    AGCTCCCAGC TGAGCACGCT GAAGGTGGAT ACGAAACTGC AAAGGGCCAG TGTGGCGGAG
    GCCATCACGA GACCAGTTCC CCGGGGCTAT CAGCCGCTAA AGGCCGAACC AAGTGCCAGC
    CAAAGTATTG CCAAAGAACA AGGATCAGCA TCAGGATCCG GATCGGGATC GGGAAGTGCT
    AGTGGTAGCG GAAGCGGTGG TAGCACCAAT TCCCCAGCGC ATAAGCGCCA GGATTCGGAC
    TCCCGATTGC CAACGAATTT TGAGCGCGGT TTTCGACGCG AGAACTCGGA CTTCTTCCCG
    CTGGCCAAGA GATACTCGGC TGTGTTCAGT GGTGCAAGTG CATCCGGATC AACAGCTGGA
    TCGCCGTCAG CACAGGCTCT TCAGAGGTCG AGCGCAGTGT ACCAAAGGAA CAGCATTTAT
    AGCAGCAGCA TCAGCAGTAA GAGCAAGGAG AATGACGTGC CTGGAGCGCC AGGAGCAGCT
    GCAGCAGGAA CTGCCACTGC CAGTGCCAAG CCAGGACCAG CTGCAGCCAC AACCACTGCC
    ACCAAGGGAG CTGCTCCAAA GACCTCCAAG TCCCTGGCCA ACTTTCATTT CCTGCGACCG
    CGTCGCGAAA AGACTGAATC CGTCATTGTG CTGCAGAATG CTGCCGTTCG CGCCCAAAGG
    CTGCAGCAAC TGCAACAGCA GCAGCAGCAG CAGCAACTGC AGCAGCAACA GCAGCAGCAG
    AATCGAGGAG GAGGCGGTGG CGGCGGCGGC GGCGGAGGAG GCGGTGGCAG CAGCGGCGTG
    GGCGCCGATG GCACTGGACT GGGAACTCCT GGGACAAGGA CCAGCAGCGT CCTGCCGGAT
    CTACTCAGCC AGGCATCGCC GGCAACGCCA CCACGCCATG ATAAATCATC CAGTGAGGAG
    TATCAAGCGG CCATTAGCTC ATCCGTGCAT TCCACGCCAT CGAAATCGTT TATCGCCAGC
    AGCGGAAGCG GTGGTCTCGG TTTGGGGGGC GGTACCGTTG TGGGCGGCGT GATTATCAGC
    AGCAATCACT CGCCCCAATC GACGATATCG CTCGCCTCTT CGTCCTCGAA TTCGCGCCAG
    AATTCGCCCA AGAATTCGAT CAGCAAGACG CGCTCCTCCA GCAGTATTAC TAATCTCTTG
    CACAAATCTG CTTCTTCCTC CTCCGCGAAC CTGCACCACC TGACGCCCTG CTCCTCGACG
    TCCTCGGCCT CGATCTCCAA TCCGCTGCCA CCGCACGCCT ATGCCCTGCA ACAGAAGCAG
    CGCAGTTTCC TGACCTTTGG CTTCGGCGCC GGCGGCTCTG GTCCTTCGCG CCGGGAATCG
    CACGTCAATG TCAATGTAAC GCCCACCTCC CACGAGGCGG CCAACGATAC GCCCGAGATC
    CGTAAGTATA AGAAGCGCTT CAATTCGGAA ATCCTGTGCG CAGCGCTATG GGGCGTCAAC
    CTGCTGATTG GAACGGAGAA TGGCTTAATG CTGCTAGATC GATCCGGTCA GGGCAAGGTC
    TACCAGCTCA TCTCGCGACG CCGTTTCCAG CAAATGGAGG TGCTAGAGGG CCAGAACATA
    TTGGTCACCA TATCCGGCAA GAAGAACCGA GTGCGCGTCT ACTACTTGTC CTGGCTAAAG
    TCGAAGATCT TGCGCACCGA CGGACTCTCC GATCAAGTGG AGCGTCGGAA CGGTTGGATC
    AACGTGGGTG ATCTGCAAGG TGCTGTACAT TTTAAGATTG TCAAATACGA GAGGATTAAG
    TTCCTAGTGA TTGCATTAAA AGACTCTATT GAAATTTATG CATGGGCTCC CAAACCATAT
    CACAAATTTA TGGCATTTAA GAATTTTGGT GAACTGGAAC ATCGCCCGCT TTTGGTCGAT
    CTCACCATTG AGGATCAGTC GAGACTGAAG GTGATCTATG GCTCCGCCGA GGGTTTCCAT
    GCGGTTGATT TAGATTCAGC TGAAGTATAC GATATCTATC TTCCCAAGCA TACTCAGGGT
    GCAATCATTC CGCATTGTAT TGTGGCGCTT CCCAACTCAA ATGGCATGCA ACTGCTGCTG
    TGCTACGACA ATGAGGGCGT CTATGTGAAC ACTGTGGGCC GCGTTTCCAA GAACATTGTA
    CTGCAGTGGG GCGAGATGCC CACCTCGGTG GCCTACATCG GCACTGGACA AATCATGGGC
    TGGGGCAATA AAGCAATAGA GATACGTTCC GTTGAGAGCG GCCATTTGGA TGGTGTGTTC
    ATGCACAAAA AGGCGCAGCG CTTGAAATTC CTTTGCGAGC GAAACGACAA AGTATTCTTC
    AGCAGTGCCA AAGGTGCTTC ATCGTGTCAA ATCTACTTTA TGACGCTCAA CAAGCCGGGC
    ATGGCCAATT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_524679.3
    CDS195..4709
    Translation

    Target ORF information:

    RefSeq Version NM_079940.5
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misshapen (msn), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_079940.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGTTAC CACCAAGGAG ACCTGAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA
    TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG
    TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG
    CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC
    AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA
    TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC
    ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG
    CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA ACTAGAGCAG
    AACATAAACC GCCTGCAAAA GTCCGCATCC ACATCGCAAG CCAATGTGAC ACCGTCGCGT
    CGCGGCGACG AATCCAATTT GCTGCGGGAC TGGGACTTTG ATCGTTTCTT CCCCAAGAAC
    GCAAACGGTC CGAGGGGCAG TGGACGTGGT AGCCCCACAA CCACTGCCAG CTTGAGCCGA
    AGCTCCCAGC TGAGCACGCT GAAGGTGGAT ACGAAACTGC AAAGGGCCAG TGTGGCGGAG
    GCCATCACGA GACCAGTTCC CCGGGGCTAT CAGCCGCTAA AGGCCGAACC AAGTGCCAGC
    CAAAGTATTG CCAAAGAACA AGGATCAGCA TCAGGATCCG GATCGGGATC GGGAAGTGCT
    AGTGGTAGCG GAAGCGGTGG TAGCACCAAT TCCCCAGCGC ATAAGCGCCA GGATTCGGAC
    TCCCGATTGC CAACGAATTT TGAGCGCGGT TTTCGACGCG AGAACTCGGA CTTCTTCCCG
    CTGGCCAAGA GATACTCGGC TGTGTTCAGT GGTGCAAGTG CATCCGGATC AACAGCTGGA
    TCGCCGTCAG CACAGGCTCT TCAGAGGTCG AGCGCAGTGT ACCAAAGGAA CAGCATTTAT
    AGCAGCAGCA TCAGCAGTAA GAGCAAGGAG AATGACGTGC CTGGAGCGCC AGGAGCAGCT
    GCAGCAGGAA CTGCCACTGC CAGTGCCAAG CCAGGACCAG CTGCAGCCAC AACCACTGCC
    ACCAAGGGAG CTGCTCCAAA GACCTCCAAG TCCCTGGCCA ACTTTCATTT CCTGCGACCG
    CGTCGCGAAA AGACTGAATC CGTCATTGTG CTGCAGAATG CTGCCGTTCG CGCCCAAAGG
    CTGCAGCAAC TGCAACAGCA GCAGCAGCAG CAGCAACTGC AGCAGCAACA GCAGCAGCAG
    AATCGAGGAG GAGGCGGTGG CGGCGGCGGC GGCGGAGGAG GCGGTGGCAG CAGCGGCGTG
    GGCGCCGATG GCACTGGACT GGGAACTCCT GGGACAAGGA CCAGCAGCGT CCTGCCGGAT
    CTACTCAGCC AGGCATCGCC GGCAACGCCA CCACGCCATG ATAAATCATC CAGTGAGGAG
    TATCAAGCGG CCATTAGCTC ATCCGTGCAT TCCACGCCAT CGAAATCGTT TATCGCCAGC
    AGCGGAAGCG GTGGTCTCGG TTTGGGGGGC GGTACCGTTG TGGGCGGCGT GATTATCAGC
    AGCAATCACT CGCCCCAATC GACGATATCG CTCGCCTCTT CGTCCTCGAA TTCGCGCCAG
    AATTCGCCCA AGAATTCGAT CAGCAAGACG CGCTCCTCCA GCAGTATTAC TAATCTCTTG
    CACAAATCTG CTTCTTCCTC CTCCGCGAAC CTGCACCACC TGACGCCCTG CTCCTCGACG
    TCCTCGGCCT CGATCTCCAA TCCGCTGCCA CCGCACGCCT ATGCCCTGCA ACAGAAGCAG
    CGCAGTTTCC TGACCTTTGG CTTCGGCGCC GGCGGCTCTG GTCCTTCGCG CCGGGAATCG
    CACGTCAATG TCAATGTAAC GCCCACCTCC CACGAGGCGG CCAACGATAC GCCCGAGATC
    CGTAAGTATA AGAAGCGCTT CAATTCGGAA ATCCTGTGCG CAGCGCTATG GGGCGTCAAC
    CTGCTGATTG GAACGGAGAA TGGCTTAATG CTGCTAGATC GATCCGGTCA GGGCAAGGTC
    TACCAGCTCA TCTCGCGACG CCGTTTCCAG CAAATGGAGG TGCTAGAGGG CCAGAACATA
    TTGGTCACCA TATCCGGCAA GAAGAACCGA GTGCGCGTCT ACTACTTGTC CTGGCTAAAG
    TCGAAGATCT TGCGCACCGA CGGACTCTCC GATCAAGTGG AGCGTCGGAA CGGTTGGATC
    AACGTGGGTG ATCTGCAAGG TGCTGTACAT TTTAAGATTG TCAAATACGA GAGGATTAAG
    TTCCTAGTGA TTGCATTAAA AGACTCTATT GAAATTTATG CATGGGCTCC CAAACCATAT
    CACAAATTTA TGGCATTTAA GAATTTTGGT GAACTGGAAC ATCGCCCGCT TTTGGTCGAT
    CTCACCATTG AGGATCAGTC GAGACTGAAG GTGATCTATG GCTCCGCCGA GGGTTTCCAT
    GCGGTTGATT TAGATTCAGC TGAAGTATAC GATATCTATC TTCCCAAGCA TACTCAGGGT
    GCAATCATTC CGCATTGTAT TGTGGCGCTT CCCAACTCAA ATGGCATGCA ACTGCTGCTG
    TGCTACGACA ATGAGGGCGT CTATGTGAAC ACTGTGGGCC GCGTTTCCAA GAACATTGTA
    CTGCAGTGGG GCGAGATGCC CACCTCGGTG GCCTACATCG GCACTGGACA AATCATGGGC
    TGGGGCAATA AAGCAATAGA GATACGTTCC GTTGAGAGCG GCCATTTGGA TGGTGTGTTC
    ATGCACAAAA AGGCGCAGCG CTTGAAATTC CTTTGCGAGC GAAACGACAA AGTATTCTTC
    AGCAGTGCCA AAGGTGCTTC ATCGTGTCAA ATCTACTTTA TGACGCTCAA CAAGCCGGGC
    ATGGCCAATT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20457
    Clone ID Related Accession (Same CDS sequence) NM_206249.3
    Accession Version NM_206249.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misshapen, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206249.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT
    GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA
    TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG
    TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG
    CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC
    AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA
    TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC
    ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG
    CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA
    GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC
    ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG
    GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGCA GCGCAGTTTC
    CTGACCTTTG GCTTCGGCGC CGGCGGCTCT GGTCCTTCGC GCCGGGAATC GCACGTCAAT
    GTCAATGTAA CGCCCACCTC CCACGAGGCG GCCAACGATA CGCCCGAGAT CCGTAAGTAT
    AAGAAGCGCT TCAATTCGGA AATCCTGTGC GCAGCGCTAT GGGGCGTCAA CCTGCTGATT
    GGAACGGAGA ATGGCTTAAT GCTGCTAGAT CGATCCGGTC AGGGCAAGGT CTACCAGCTC
    ATCTCGCGAC GCCGTTTCCA GCAAATGGAG GTGCTAGAGG GCCAGAACAT ATTGGTCACC
    ATATCCGGCA AGAAGAACCG AGTGCGCGTC TACTACTTGT CCTGGCTAAA GTCGAAGATC
    TTGCGCACCG ACGGACTCTC CGATCAAGTG GAGCGTCGGA ACGGTTGGAT CAACGTGGGT
    GATCTGCAAG GTGCTGTACA TTTTAAGATT GTCAAATACG AGAGGATTAA GTTCCTAGTG
    ATTGCATTAA AAGACTCTAT TGAAATTTAT GCATGGGCTC CCAAACCATA TCACAAATTT
    ATGGCATTTA AGAATTTTGG TGAACTGGAA CATCGCCCGC TTTTGGTCGA TCTCACCATT
    GAGGATCAGT CGAGACTGAA GGTGATCTAT GGCTCCGCCG AGGGTTTCCA TGCGGTTGAT
    TTAGATTCAG CTGAAGTATA CGATATCTAT CTTCCCAAGC ATACTCAGGG TGCAATCATT
    CCGCATTGTA TTGTGGCGCT TCCCAACTCA AATGGCATGC AACTGCTGCT GTGCTACGAC
    AATGAGGGCG TCTATGTGAA CACTGTGGGC CGCGTTTCCA AGAACATTGT ACTGCAGTGG
    GGCGAGATGC CCACCTCGGT GGCCTACATC GGCACTGGAC AAATCATGGG CTGGGGCAAT
    AAAGCAATAG AGATACGTTC CGTTGAGAGC GGCCATTTGG ATGGTGTGTT CATGCACAAA
    AAGGCGCAGC GCTTGAAATT CCTTTGCGAG CGAAACGACA AAGTATTCTT CAGCAGTGCC
    AAAGGTGCTT CATCGTGTCA AATCTACTTT ATGACGCTCA ACAAGCCGGG CATGGCCAAT
    TGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_995971.2
    CDS195..3500
    Translation

    Target ORF information:

    RefSeq Version NM_206249.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misshapen (msn), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206249.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
    GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT
    GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA
    AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA
    AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG
    CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA
    ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC
    TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC
    ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT
    GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC
    TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC
    TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC
    TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC
    AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC
    TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC
    ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG
    CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA
    CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC
    AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG
    ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA
    CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA
    CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA
    AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC
    CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC
    GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG
    CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT
    GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA
    TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG
    TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG
    CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC
    AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA
    TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC
    ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG
    CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA
    GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC
    ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG
    GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGCA GCGCAGTTTC
    CTGACCTTTG GCTTCGGCGC CGGCGGCTCT GGTCCTTCGC GCCGGGAATC GCACGTCAAT
    GTCAATGTAA CGCCCACCTC CCACGAGGCG GCCAACGATA CGCCCGAGAT CCGTAAGTAT
    AAGAAGCGCT TCAATTCGGA AATCCTGTGC GCAGCGCTAT GGGGCGTCAA CCTGCTGATT
    GGAACGGAGA ATGGCTTAAT GCTGCTAGAT CGATCCGGTC AGGGCAAGGT CTACCAGCTC
    ATCTCGCGAC GCCGTTTCCA GCAAATGGAG GTGCTAGAGG GCCAGAACAT ATTGGTCACC
    ATATCCGGCA AGAAGAACCG AGTGCGCGTC TACTACTTGT CCTGGCTAAA GTCGAAGATC
    TTGCGCACCG ACGGACTCTC CGATCAAGTG GAGCGTCGGA ACGGTTGGAT CAACGTGGGT
    GATCTGCAAG GTGCTGTACA TTTTAAGATT GTCAAATACG AGAGGATTAA GTTCCTAGTG
    ATTGCATTAA AAGACTCTAT TGAAATTTAT GCATGGGCTC CCAAACCATA TCACAAATTT
    ATGGCATTTA AGAATTTTGG TGAACTGGAA CATCGCCCGC TTTTGGTCGA TCTCACCATT
    GAGGATCAGT CGAGACTGAA GGTGATCTAT GGCTCCGCCG AGGGTTTCCA TGCGGTTGAT
    TTAGATTCAG CTGAAGTATA CGATATCTAT CTTCCCAAGC ATACTCAGGG TGCAATCATT
    CCGCATTGTA TTGTGGCGCT TCCCAACTCA AATGGCATGC AACTGCTGCT GTGCTACGAC
    AATGAGGGCG TCTATGTGAA CACTGTGGGC CGCGTTTCCA AGAACATTGT ACTGCAGTGG
    GGCGAGATGC CCACCTCGGT GGCCTACATC GGCACTGGAC AAATCATGGG CTGGGGCAAT
    AAAGCAATAG AGATACGTTC CGTTGAGAGC GGCCATTTGG ATGGTGTGTT CATGCACAAA
    AAGGCGCAGC GCTTGAAATT CCTTTGCGAG CGAAACGACA AAGTATTCTT CAGCAGTGCC
    AAAGGTGCTT CATCGTGTCA AATCTACTTT ATGACGCTCA ACAAGCCGGG CATGGCCAAT
    TGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.