GEMIN4 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010815869.2	XP_010814171.1	gem-associated protein 4 isoform X1
XM_010815870.2	XP_010814172.1	gem-associated protein 4 isoform X3
XM_024980697.1	XP_024836465.1	gem-associated protein 4 isoform X1
XM_585199.8	XP_585199.4	gem-associated protein 4 isoform X2
XM_010824017.2	XP_010822319.1	gem-associated protein 4 isoform X3
XM_002695679.4	XP_002695725.1	gem-associated protein 4 isoform X2
XM_010824016.2	XP_010822318.2	gem-associated protein 4 isoform X1
XM_010815869.3	XP_010814171.2	gem-associated protein 4 isoform X1
XM_002695679.5	XP_002695725.1	gem-associated protein 4 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa10037	XM_024980697.1 Latest version!	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa10037	XM_010815869.3 Latest version!	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa10038	XM_585199.8 Latest version!	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa10038	XM_002695679.5 Latest version!	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa29565	XM_010815869.2 Latest version!	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa29565	XM_010824016.2 Latest version!	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa54209	XM_010815870.2 Latest version!	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa54209	XM_010824017.2 Latest version!	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa10038	XM_002695679.4	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

GEMIN4 ( XM_024980697.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836465.1 Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa10037
Clone ID Related Accession (Same CDS sequence)	XM_024980697.1 , XM_010815869.3
Accession Version	XM_024980697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	gem-associated protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGAAGTG GTGTGTCCAT 
GCAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT 
GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT 
GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC 
TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC 
ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC 
ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT 
GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG 
CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC 
GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC 
CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG 
TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG 
CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC 
TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG 
ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC 
CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG 
ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC 
CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC 
TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC 
CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC 
GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC 
ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG 
TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG 
CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG 
ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC 
TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG 
GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC 
TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC 
TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG 
AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT 
CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA 
TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG 
AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA 
GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG 
CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC 
ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG 
TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC 
CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA 
GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG 
AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC 
TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG 
ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC 
TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC 
AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG 
CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG 
AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC 
CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT 
CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG 
CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC 
CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG 
GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC 
TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC 
GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC 
ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836465.1
CDS	259..3492
Translation	MRGTGAPLRGRKALRKWCVHAGPLNICEEMTILHGGFLLAEQLFRPKALAELTKSDWVHVGRPIVEALREISSTTACSQPFAWKKKALIIIWAKVLQPYPITPSDMETRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTVCRAELEHFLEHMTIDTSSKDVAFFLDVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDDFSHPPKRFKSDPDVCPTMPLLAMLLTGLKQIQDRILCPGMKCCALANLADMLTVFALMEEDPQEVSATVYLDKLATVISVWNSDTQNPYHQQALAEKVKEAERDISLTSLARLPSETIFVGFEFLRSLLQEWGEELQALLSSSQGTSYDSFRLCDSLTSFSQNLKLYLDTTSLAKEERQVVSELAECVGDFLRKTNRVVRSKGGEQDITASIAMAIIEQKMDRHMEMCYIFASERKWAFSDEWLSCLLNNRALFREPGLVLKLLETVMEVSTIDRAVSEPQIKQVFDLILECYTHLSLPDKNKVLSGVLLSWGRKGLSEKLLAHLEGFQEDLNTTFNQLTQSTSEQGLAKAVASVARLVILHPEITVKKLCSMAVVNLGTHKFLAQILTAFPALRFMEEQGPDRSTTFVVSCLKETVWTKLSTPREEKQFLELLSCLVSPTKPQGIPVAALLEPDEVLKEFVLPFLMLEVKEVDLSLRIFVQTLEGSTNLEEYWLQSCSPFPLIFSLCQLLDGFSKYWQLPKEKRCLPLDGKDLAIHILELLCEVVSANTDTFSPDTWVKSLSWLHRKLEQLDWTVGLRLKSFFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECSSISSSVSEQMLALLVVDVGNPEEVRLFSKGFLVALVQVMPWCSPQEWQCLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQLSVLLLRVFQFLCSQSCRNWLPVEGWSHVVKLLCNSLTNLLDSVRLIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMVHQEVCEPLYVLALEILTCYETLSKTNPSVSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_024980697.1
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980697.1

ORF Insert Sequence:

ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGAAGTG GTGTGTCCAT 
GCAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT 
GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT 
GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC 
TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC 
ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC 
ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT 
GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG 
CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC 
GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC 
CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG 
TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG 
CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC 
TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG 
ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC 
CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG 
ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC 
CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC 
TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC 
CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC 
GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC 
ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG 
TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG 
CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG 
ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC 
TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG 
GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC 
TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC 
TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG 
AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT 
CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA 
TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG 
AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA 
GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG 
CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC 
ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG 
TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC 
CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA 
GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG 
AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC 
TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG 
ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC 
TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC 
AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG 
CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG 
AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC 
CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT 
CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG 
CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC 
CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG 
GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC 
TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC 
GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC 
ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_010815869.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814171.2 Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa10037
Clone ID Related Accession (Same CDS sequence)	XM_024980697.1 , XM_010815869.3
Accession Version	XM_010815869.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	gem-associated protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815869.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGAAGTG GTGTGTCCAT 
GCAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT 
GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT 
GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC 
TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC 
ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC 
ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT 
GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG 
CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC 
GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC 
CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG 
TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG 
CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC 
TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG 
ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC 
CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG 
ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC 
CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC 
TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC 
CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC 
GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC 
ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG 
TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG 
CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG 
ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC 
TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG 
GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC 
TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC 
TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG 
AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT 
CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA 
TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG 
AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA 
GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG 
CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC 
ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG 
TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC 
CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA 
GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG 
AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC 
TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG 
ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC 
TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC 
AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG 
CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG 
AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC 
CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT 
CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG 
CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC 
CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG 
GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC 
TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC 
GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC 
ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814171.2
CDS	276..3509
Translation	MRGTGAPLRGRKALRKWCVHAGPLNICEEMTILHGGFLLAEQLFRPKALAELTKSDWVHVGRPIVEALREISSTTACSQPFAWKKKALIIIWAKVLQPYPITPSDMETRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTVCRAELEHFLEHMTIDTSSKDVAFFLDVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDDFSHPPKRFKSDPDVCPTMPLLAMLLTGLKQIQDRILCPGMKCCALANLADMLTVFALMEEDPQEVSATVYLDKLATVISVWNSDTQNPYHQQALAEKVKEAERDISLTSLARLPSETIFVGFEFLRSLLQEWGEELQALLSSSQGTSYDSFRLCDSLTSFSQNLKLYLDTTSLAKEERQVVSELAECVGDFLRKTNRVVRSKGGEQDITASIAMAIIEQKMDRHMEMCYIFASERKWAFSDEWLSCLLNNRALFREPGLVLKLLETVMEVSTIDRAVSEPQIKQVFDLILECYTHLSLPDKNKVLSGVLLSWGRKGLSEKLLAHLEGFQEDLNTTFNQLTQSTSEQGLAKAVASVARLVILHPEITVKKLCSMAVVNLGTHKFLAQILTAFPALRFMEEQGPDRSTTFVVSCLKETVWTKLSTPREEKQFLELLSCLVSPTKPQGIPVAALLEPDEVLKEFVLPFLMLEVKEVDLSLRIFVQTLEGSTNLEEYWLQSCSPFPLIFSLCQLLDGFSKYWQLPKEKRCLPLDGKDLAIHILELLCEVVSANTDTFSPDTWVKSLSWLHRKLEQLDWTVGLRLKSFFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECSSISSSVSEQMLALLVVDVGNPEEVRLFSKGFLVALVQVMPWCSPQEWQCLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQLSVLLLRVFQFLCSQSCRNWLPVEGWSHVVKLLCNSLTNLLDSVRLIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMVHQEVCEPLYVLALEILTCYETLSKTNPSVSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_010815869.3
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815869.3

ORF Insert Sequence:

ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGAAGTG GTGTGTCCAT 
GCAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT 
GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT 
GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC 
TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC 
ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC 
ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT 
GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG 
CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC 
GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC 
CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG 
TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG 
CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC 
TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG 
ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC 
CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG 
ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC 
CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC 
TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC 
CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC 
GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC 
ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG 
TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG 
CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG 
ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC 
TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG 
GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC 
TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC 
TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG 
AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT 
CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA 
TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG 
AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA 
GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG 
CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC 
ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG 
TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC 
CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA 
GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG 
AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC 
TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG 
ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC 
TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC 
AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG 
CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG 
AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC 
CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT 
CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG 
CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC 
CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG 
GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC 
TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC 
GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC 
ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_585199.8 ) cDNA ORF clone, Bos taurus(cattle) -> XP_585199.4 Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.

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CloneID	OBa10038
Clone ID Related Accession (Same CDS sequence)	XM_585199.8 , XM_002695679.4 , XM_002695679.5
Accession Version	XM_585199.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	gem-associated protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_585199.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTAG GACCTTTGAA TATCTGTGAG GAAATGACTA TCTTGCACGG GGGCTTCTTG 
CTGGCTGAGC AGCTGTTCCG CCCCAAAGCG CTGGCCGAAC TGACCAAGTC TGACTGGGTG 
CACGTTGGGC GGCCCATTGT GGAGGCTCTG AGGGAGATCT CCTCCACCAC AGCCTGCTCC 
CAGCCCTTCG CCTGGAAGAA GAAAGCGCTG ATCATCATCT GGGCCAAGGT GCTACAGCCC 
TACCCCATCA CCCCTTCCGA CATGGAAACC CGCTGGCAGG AAGATGTGTT CTTCTCTGTG 
GGCAACATGA TCCCCACCAT CAATCACACA GTCCTCTTTG AGCTGCTCAA GTCGCTGGAG 
GCCTCTGGAC TCTTTATCCA GCTCCTGATG GCCCTGCCCA CCACTGTCTG CCGTGCGGAA 
CTAGAGCACT TTTTGGAGCA CATGACCATC GACACCTCTT CCAAGGACGT GGCCTTCTTC 
CTCGACGTCT GGTGGGAAAT GATGAAGCAC AAGGGCAATC AGCAGGATCC CCTGCTCTCC 
CAGTTCCGGA CAATGGCCCA CAAGTACCTG TCCTCCTCCG ATGACTTCTC CCACCCGCCG 
AAGAGGTTCA AGTCCGATCC AGATGTGTGT CCAACCATGC CCCTGCTGGC CATGCTGCTC 
ACGGGGCTGA AACAAATCCA GGACAGGATC CTATGCCCCG GGATGAAGTG CTGCGCCTTA 
GCCAACTTGG CTGACATGCT GACCGTGTTT GCGCTGATGG AGGAGGACCC CCAGGAAGTG 
TCTGCGACCG TGTACCTGGA CAAGCTGGCC ACGGTGATCT CCGTGTGGAA CTCGGACACC 
CAGAACCCGT ATCACCAGCA GGCGCTGGCA GAGAAGGTGA AGGAGGCGGA GCGGGACATC 
AGCCTGACCT CCCTGGCCAG GCTGCCGAGC GAGACCATCT TCGTGGGCTT TGAGTTCCTG 
CGCAGCCTGC TGCAGGAGTG GGGGGAGGAG CTGCAGGCCT TGCTCAGCAG CAGCCAGGGG 
ACCAGCTACG ACAGCTTCCG GCTCTGCGAC AGCCTGACCT CCTTCAGCCA GAATCTGAAG 
CTCTACCTGG ACACCACCAG CCTGGCCAAG GAGGAGAGGC AGGTGGTCTC CGAGCTGGCA 
GAGTGCGTGG GGGACTTCCT GAGGAAGACG AACAGGGTGG TGAGGAGCAA GGGCGGTGAG 
CAGGACATCA CCGCCTCCAT CGCCATGGCC ATCATCGAGC AGAAGATGGA CCGCCACATG 
GAAATGTGCT ACATTTTTGC CTCCGAGAGG AAGTGGGCCT TCTCGGACGA GTGGCTGTCC 
TGCCTGCTCA ACAACCGAGC TCTCTTCCGA GAGCCGGGCC TGGTGTTAAA GCTGCTGGAG 
ACGGTGATGG AAGTCAGCAC GATAGACAGG GCTGTCTCCG AACCTCAGAT CAAACAGGTG 
TTCGACTTGA TCCTGGAGTG TTACACACAC CTCTCTCTGC CAGATAAAAA TAAAGTCCTC 
TCGGGGGTCC TGCTCTCCTG GGGGCGCAAG GGCCTCTCTG AGAAATTGTT AGCTCACTTG 
GAGGGCTTTC AGGAGGACCT CAACACGACT TTCAACCAGC TCACACAGAG CACCTCTGAA 
CAGGGCTTGG CTAAGGCTGT TGCTTCCGTG GCCCGCCTGG TCATCCTGCA CCCAGAGATC 
ACGGTGAAGA AGTTGTGCAG CATGGCTGTG GTCAACCTCG GCACCCACAA GTTCCTGGCT 
CAGATTCTCA CTGCCTTCCC CGCTCTCCGG TTCATGGAAG AGCAGGGGCC AGACCGCTCT 
ACCACATTCG TGGTGTCCTG CCTCAAAGAA ACGGTCTGGA CCAAGCTCTC GACACCCCGG 
GAGGAGAAGC AGTTTCTGGA GCTCTTGAGC TGCCTGGTGA GTCCTACAAA GCCCCAAGGG 
ATTCCAGTCG CTGCTCTGCT CGAGCCGGAT GAGGTACTAA AGGAGTTCGT CCTGCCTTTC 
TTGATGCTGG AGGTCAAAGA GGTGGACCTC AGTCTGAGGA TCTTCGTCCA GACTCTGGAA 
GGGAGCACAA ACCTGGAAGA ATACTGGCTG CAGTCCTGCT CTCCGTTCCC GCTCATCTTC 
AGCCTGTGCC AGCTCCTTGA TGGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAGCGC 
TGTCTCCCCC TGGACGGGAA GGACCTGGCC ATCCACATCC TGGAGCTCCT CTGTGAGGTG 
GTCTCAGCTA ACACCGACAC CTTCTCCCCA GACACCTGGG TCAAGTCGCT GTCCTGGCTC 
CACCGGAAGC TGGAGCAGCT AGACTGGACT GTGGGCCTGA GACTGAAGAG CTTCTTTGAG 
GGCCACTTCA AGTGCGAGGT GCCAGCCACG CTCTTTGAGA TCTGTAAGCT TTCTGAGGAC 
GAGTGGACCT CCCAGGCCCA CCCCGGGTAT GGGCCTGGCA CAGGCCTCCT GGCCTGGATG 
GAGTGCTCCT CCATCTCCAG CAGCGTCTCC GAGCAGATGC TCGCACTCCT GGTGGTGGAC 
GTGGGCAACC CTGAGGAGGT CAGGTTGTTC AGCAAGGGCT TCCTGGTGGC CCTGGTACAG 
GTCATGCCAT GGTGCAGCCC CCAGGAATGG CAGTGCCTTC ACCAGCTGAC CCGGAGACTG 
TTGGAGAAGC AACTCCTGCA CGTCCCCTAC AGCCTGGAGT ATATACAGTT CGTTCCTCTG 
CTCAACCTGA AGCCCTTTGC CCAGGAGCTC CAGCTCTCTG TCCTCTTGCT TAGAGTTTTT 
CAGTTTCTCT GCAGCCAGAG CTGCCGTAAC TGGCTCCCTG TGGAGGGCTG GAGCCATGTG 
GTCAAGCTCC TCTGCAACAG TCTGACCAAT CTCCTGGACT CTGTTCGGTT GATACAGTCG 
GTTGGCCCTT GGGCTCAAGG ACAAGAGCAG GACCTGACCC AGGAAACCCT CTTTTTCTAT 
ACCCAGGTCT TCTGTCATGT TCTGCACATC ATGGCCATGG TCCACCAGGA GGTCTGTGAA 
CCTCTCTATG TCTTGGCCTT GGAGATCCTC ACCTGCTACG AAACCCTGAG CAAGACCAAC 
CCTTCCGTCA GCTCCTTGCT CCAGAAAGTA AACGAGCAGC GCTTCTTAAA GTCCATCGCC 
GAGAACATCA GCCCTGAGGA GCGGCGCCAA ACCCTCCTGC AGAAGATCAG CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_585199.4
CDS	184..3363
Translation	MDLGPLNICEEMTILHGGFLLAEQLFRPKALAELTKSDWVHVGRPIVEALREISSTTACSQPFAWKKKALIIIWAKVLQPYPITPSDMETRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTVCRAELEHFLEHMTIDTSSKDVAFFLDVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDDFSHPPKRFKSDPDVCPTMPLLAMLLTGLKQIQDRILCPGMKCCALANLADMLTVFALMEEDPQEVSATVYLDKLATVISVWNSDTQNPYHQQALAEKVKEAERDISLTSLARLPSETIFVGFEFLRSLLQEWGEELQALLSSSQGTSYDSFRLCDSLTSFSQNLKLYLDTTSLAKEERQVVSELAECVGDFLRKTNRVVRSKGGEQDITASIAMAIIEQKMDRHMEMCYIFASERKWAFSDEWLSCLLNNRALFREPGLVLKLLETVMEVSTIDRAVSEPQIKQVFDLILECYTHLSLPDKNKVLSGVLLSWGRKGLSEKLLAHLEGFQEDLNTTFNQLTQSTSEQGLAKAVASVARLVILHPEITVKKLCSMAVVNLGTHKFLAQILTAFPALRFMEEQGPDRSTTFVVSCLKETVWTKLSTPREEKQFLELLSCLVSPTKPQGIPVAALLEPDEVLKEFVLPFLMLEVKEVDLSLRIFVQTLEGSTNLEEYWLQSCSPFPLIFSLCQLLDGFSKYWQLPKEKRCLPLDGKDLAIHILELLCEVVSANTDTFSPDTWVKSLSWLHRKLEQLDWTVGLRLKSFFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECSSISSSVSEQMLALLVVDVGNPEEVRLFSKGFLVALVQVMPWCSPQEWQCLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQLSVLLLRVFQFLCSQSCRNWLPVEGWSHVVKLLCNSLTNLLDSVRLIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMVHQEVCEPLYVLALEILTCYETLSKTNPSVSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_585199.8
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_585199.8

ORF Insert Sequence:

ATGGACCTAG GACCTTTGAA TATCTGTGAG GAAATGACTA TCTTGCACGG GGGCTTCTTG 
CTGGCTGAGC AGCTGTTCCG CCCCAAAGCG CTGGCCGAAC TGACCAAGTC TGACTGGGTG 
CACGTTGGGC GGCCCATTGT GGAGGCTCTG AGGGAGATCT CCTCCACCAC AGCCTGCTCC 
CAGCCCTTCG CCTGGAAGAA GAAAGCGCTG ATCATCATCT GGGCCAAGGT GCTACAGCCC 
TACCCCATCA CCCCTTCCGA CATGGAAACC CGCTGGCAGG AAGATGTGTT CTTCTCTGTG 
GGCAACATGA TCCCCACCAT CAATCACACA GTCCTCTTTG AGCTGCTCAA GTCGCTGGAG 
GCCTCTGGAC TCTTTATCCA GCTCCTGATG GCCCTGCCCA CCACTGTCTG CCGTGCGGAA 
CTAGAGCACT TTTTGGAGCA CATGACCATC GACACCTCTT CCAAGGACGT GGCCTTCTTC 
CTCGACGTCT GGTGGGAAAT GATGAAGCAC AAGGGCAATC AGCAGGATCC CCTGCTCTCC 
CAGTTCCGGA CAATGGCCCA CAAGTACCTG TCCTCCTCCG ATGACTTCTC CCACCCGCCG 
AAGAGGTTCA AGTCCGATCC AGATGTGTGT CCAACCATGC CCCTGCTGGC CATGCTGCTC 
ACGGGGCTGA AACAAATCCA GGACAGGATC CTATGCCCCG GGATGAAGTG CTGCGCCTTA 
GCCAACTTGG CTGACATGCT GACCGTGTTT GCGCTGATGG AGGAGGACCC CCAGGAAGTG 
TCTGCGACCG TGTACCTGGA CAAGCTGGCC ACGGTGATCT CCGTGTGGAA CTCGGACACC 
CAGAACCCGT ATCACCAGCA GGCGCTGGCA GAGAAGGTGA AGGAGGCGGA GCGGGACATC 
AGCCTGACCT CCCTGGCCAG GCTGCCGAGC GAGACCATCT TCGTGGGCTT TGAGTTCCTG 
CGCAGCCTGC TGCAGGAGTG GGGGGAGGAG CTGCAGGCCT TGCTCAGCAG CAGCCAGGGG 
ACCAGCTACG ACAGCTTCCG GCTCTGCGAC AGCCTGACCT CCTTCAGCCA GAATCTGAAG 
CTCTACCTGG ACACCACCAG CCTGGCCAAG GAGGAGAGGC AGGTGGTCTC CGAGCTGGCA 
GAGTGCGTGG GGGACTTCCT GAGGAAGACG AACAGGGTGG TGAGGAGCAA GGGCGGTGAG 
CAGGACATCA CCGCCTCCAT CGCCATGGCC ATCATCGAGC AGAAGATGGA CCGCCACATG 
GAAATGTGCT ACATTTTTGC CTCCGAGAGG AAGTGGGCCT TCTCGGACGA GTGGCTGTCC 
TGCCTGCTCA ACAACCGAGC TCTCTTCCGA GAGCCGGGCC TGGTGTTAAA GCTGCTGGAG 
ACGGTGATGG AAGTCAGCAC GATAGACAGG GCTGTCTCCG AACCTCAGAT CAAACAGGTG 
TTCGACTTGA TCCTGGAGTG TTACACACAC CTCTCTCTGC CAGATAAAAA TAAAGTCCTC 
TCGGGGGTCC TGCTCTCCTG GGGGCGCAAG GGCCTCTCTG AGAAATTGTT AGCTCACTTG 
GAGGGCTTTC AGGAGGACCT CAACACGACT TTCAACCAGC TCACACAGAG CACCTCTGAA 
CAGGGCTTGG CTAAGGCTGT TGCTTCCGTG GCCCGCCTGG TCATCCTGCA CCCAGAGATC 
ACGGTGAAGA AGTTGTGCAG CATGGCTGTG GTCAACCTCG GCACCCACAA GTTCCTGGCT 
CAGATTCTCA CTGCCTTCCC CGCTCTCCGG TTCATGGAAG AGCAGGGGCC AGACCGCTCT 
ACCACATTCG TGGTGTCCTG CCTCAAAGAA ACGGTCTGGA CCAAGCTCTC GACACCCCGG 
GAGGAGAAGC AGTTTCTGGA GCTCTTGAGC TGCCTGGTGA GTCCTACAAA GCCCCAAGGG 
ATTCCAGTCG CTGCTCTGCT CGAGCCGGAT GAGGTACTAA AGGAGTTCGT CCTGCCTTTC 
TTGATGCTGG AGGTCAAAGA GGTGGACCTC AGTCTGAGGA TCTTCGTCCA GACTCTGGAA 
GGGAGCACAA ACCTGGAAGA ATACTGGCTG CAGTCCTGCT CTCCGTTCCC GCTCATCTTC 
AGCCTGTGCC AGCTCCTTGA TGGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAGCGC 
TGTCTCCCCC TGGACGGGAA GGACCTGGCC ATCCACATCC TGGAGCTCCT CTGTGAGGTG 
GTCTCAGCTA ACACCGACAC CTTCTCCCCA GACACCTGGG TCAAGTCGCT GTCCTGGCTC 
CACCGGAAGC TGGAGCAGCT AGACTGGACT GTGGGCCTGA GACTGAAGAG CTTCTTTGAG 
GGCCACTTCA AGTGCGAGGT GCCAGCCACG CTCTTTGAGA TCTGTAAGCT TTCTGAGGAC 
GAGTGGACCT CCCAGGCCCA CCCCGGGTAT GGGCCTGGCA CAGGCCTCCT GGCCTGGATG 
GAGTGCTCCT CCATCTCCAG CAGCGTCTCC GAGCAGATGC TCGCACTCCT GGTGGTGGAC 
GTGGGCAACC CTGAGGAGGT CAGGTTGTTC AGCAAGGGCT TCCTGGTGGC CCTGGTACAG 
GTCATGCCAT GGTGCAGCCC CCAGGAATGG CAGTGCCTTC ACCAGCTGAC CCGGAGACTG 
TTGGAGAAGC AACTCCTGCA CGTCCCCTAC AGCCTGGAGT ATATACAGTT CGTTCCTCTG 
CTCAACCTGA AGCCCTTTGC CCAGGAGCTC CAGCTCTCTG TCCTCTTGCT TAGAGTTTTT 
CAGTTTCTCT GCAGCCAGAG CTGCCGTAAC TGGCTCCCTG TGGAGGGCTG GAGCCATGTG 
GTCAAGCTCC TCTGCAACAG TCTGACCAAT CTCCTGGACT CTGTTCGGTT GATACAGTCG 
GTTGGCCCTT GGGCTCAAGG ACAAGAGCAG GACCTGACCC AGGAAACCCT CTTTTTCTAT 
ACCCAGGTCT TCTGTCATGT TCTGCACATC ATGGCCATGG TCCACCAGGA GGTCTGTGAA 
CCTCTCTATG TCTTGGCCTT GGAGATCCTC ACCTGCTACG AAACCCTGAG CAAGACCAAC 
CCTTCCGTCA GCTCCTTGCT CCAGAAAGTA AACGAGCAGC GCTTCTTAAA GTCCATCGCC 
GAGAACATCA GCCCTGAGGA GCGGCGCCAA ACCCTCCTGC AGAAGATCAG CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_002695679.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002695725.1 Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa10038
Clone ID Related Accession (Same CDS sequence)	XM_585199.8 , XM_002695679.4 , XM_002695679.5
Accession Version	XM_002695679.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	gem-associated protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002695679.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTAG GACCTTTGAA TATCTGTGAG GAAATGACTA TCTTGCACGG GGGCTTCTTG 
CTGGCTGAGC AGCTGTTCCG CCCCAAAGCG CTGGCCGAAC TGACCAAGTC TGACTGGGTG 
CACGTTGGGC GGCCCATTGT GGAGGCTCTG AGGGAGATCT CCTCCACCAC AGCCTGCTCC 
CAGCCCTTCG CCTGGAAGAA GAAAGCGCTG ATCATCATCT GGGCCAAGGT GCTACAGCCC 
TACCCCATCA CCCCTTCCGA CATGGAAACC CGCTGGCAGG AAGATGTGTT CTTCTCTGTG 
GGCAACATGA TCCCCACCAT CAATCACACA GTCCTCTTTG AGCTGCTCAA GTCGCTGGAG 
GCCTCTGGAC TCTTTATCCA GCTCCTGATG GCCCTGCCCA CCACTGTCTG CCGTGCGGAA 
CTAGAGCACT TTTTGGAGCA CATGACCATC GACACCTCTT CCAAGGACGT GGCCTTCTTC 
CTCGACGTCT GGTGGGAAAT GATGAAGCAC AAGGGCAATC AGCAGGATCC CCTGCTCTCC 
CAGTTCCGGA CAATGGCCCA CAAGTACCTG TCCTCCTCCG ATGACTTCTC CCACCCGCCG 
AAGAGGTTCA AGTCCGATCC AGATGTGTGT CCAACCATGC CCCTGCTGGC CATGCTGCTC 
ACGGGGCTGA AACAAATCCA GGACAGGATC CTATGCCCCG GGATGAAGTG CTGCGCCTTA 
GCCAACTTGG CTGACATGCT GACCGTGTTT GCGCTGATGG AGGAGGACCC CCAGGAAGTG 
TCTGCGACCG TGTACCTGGA CAAGCTGGCC ACGGTGATCT CCGTGTGGAA CTCGGACACC 
CAGAACCCGT ATCACCAGCA GGCGCTGGCA GAGAAGGTGA AGGAGGCGGA GCGGGACATC 
AGCCTGACCT CCCTGGCCAG GCTGCCGAGC GAGACCATCT TCGTGGGCTT TGAGTTCCTG 
CGCAGCCTGC TGCAGGAGTG GGGGGAGGAG CTGCAGGCCT TGCTCAGCAG CAGCCAGGGG 
ACCAGCTACG ACAGCTTCCG GCTCTGCGAC AGCCTGACCT CCTTCAGCCA GAATCTGAAG 
CTCTACCTGG ACACCACCAG CCTGGCCAAG GAGGAGAGGC AGGTGGTCTC CGAGCTGGCA 
GAGTGCGTGG GGGACTTCCT GAGGAAGACG AACAGGGTGG TGAGGAGCAA GGGCGGTGAG 
CAGGACATCA CCGCCTCCAT CGCCATGGCC ATCATCGAGC AGAAGATGGA CCGCCACATG 
GAAATGTGCT ACATTTTTGC CTCCGAGAGG AAGTGGGCCT TCTCGGACGA GTGGCTGTCC 
TGCCTGCTCA ACAACCGAGC TCTCTTCCGA GAGCCGGGCC TGGTGTTAAA GCTGCTGGAG 
ACGGTGATGG AAGTCAGCAC GATAGACAGG GCTGTCTCCG AACCTCAGAT CAAACAGGTG 
TTCGACTTGA TCCTGGAGTG TTACACACAC CTCTCTCTGC CAGATAAAAA TAAAGTCCTC 
TCGGGGGTCC TGCTCTCCTG GGGGCGCAAG GGCCTCTCTG AGAAATTGTT AGCTCACTTG 
GAGGGCTTTC AGGAGGACCT CAACACGACT TTCAACCAGC TCACACAGAG CACCTCTGAA 
CAGGGCTTGG CTAAGGCTGT TGCTTCCGTG GCCCGCCTGG TCATCCTGCA CCCAGAGATC 
ACGGTGAAGA AGTTGTGCAG CATGGCTGTG GTCAACCTCG GCACCCACAA GTTCCTGGCT 
CAGATTCTCA CTGCCTTCCC CGCTCTCCGG TTCATGGAAG AGCAGGGGCC AGACCGCTCT 
ACCACATTCG TGGTGTCCTG CCTCAAAGAA ACGGTCTGGA CCAAGCTCTC GACACCCCGG 
GAGGAGAAGC AGTTTCTGGA GCTCTTGAGC TGCCTGGTGA GTCCTACAAA GCCCCAAGGG 
ATTCCAGTCG CTGCTCTGCT CGAGCCGGAT GAGGTACTAA AGGAGTTCGT CCTGCCTTTC 
TTGATGCTGG AGGTCAAAGA GGTGGACCTC AGTCTGAGGA TCTTCGTCCA GACTCTGGAA 
GGGAGCACAA ACCTGGAAGA ATACTGGCTG CAGTCCTGCT CTCCGTTCCC GCTCATCTTC 
AGCCTGTGCC AGCTCCTTGA TGGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAGCGC 
TGTCTCCCCC TGGACGGGAA GGACCTGGCC ATCCACATCC TGGAGCTCCT CTGTGAGGTG 
GTCTCAGCTA ACACCGACAC CTTCTCCCCA GACACCTGGG TCAAGTCGCT GTCCTGGCTC 
CACCGGAAGC TGGAGCAGCT AGACTGGACT GTGGGCCTGA GACTGAAGAG CTTCTTTGAG 
GGCCACTTCA AGTGCGAGGT GCCAGCCACG CTCTTTGAGA TCTGTAAGCT TTCTGAGGAC 
GAGTGGACCT CCCAGGCCCA CCCCGGGTAT GGGCCTGGCA CAGGCCTCCT GGCCTGGATG 
GAGTGCTCCT CCATCTCCAG CAGCGTCTCC GAGCAGATGC TCGCACTCCT GGTGGTGGAC 
GTGGGCAACC CTGAGGAGGT CAGGTTGTTC AGCAAGGGCT TCCTGGTGGC CCTGGTACAG 
GTCATGCCAT GGTGCAGCCC CCAGGAATGG CAGTGCCTTC ACCAGCTGAC CCGGAGACTG 
TTGGAGAAGC AACTCCTGCA CGTCCCCTAC AGCCTGGAGT ATATACAGTT CGTTCCTCTG 
CTCAACCTGA AGCCCTTTGC CCAGGAGCTC CAGCTCTCTG TCCTCTTGCT TAGAGTTTTT 
CAGTTTCTCT GCAGCCAGAG CTGCCGTAAC TGGCTCCCTG TGGAGGGCTG GAGCCATGTG 
GTCAAGCTCC TCTGCAACAG TCTGACCAAT CTCCTGGACT CTGTTCGGTT GATACAGTCG 
GTTGGCCCTT GGGCTCAAGG ACAAGAGCAG GACCTGACCC AGGAAACCCT CTTTTTCTAT 
ACCCAGGTCT TCTGTCATGT TCTGCACATC ATGGCCATGG TCCACCAGGA GGTCTGTGAA 
CCTCTCTATG TCTTGGCCTT GGAGATCCTC ACCTGCTACG AAACCCTGAG CAAGACCAAC 
CCTTCCGTCA GCTCCTTGCT CCAGAAAGTA AACGAGCAGC GCTTCTTAAA GTCCATCGCC 
GAGAACATCA GCCCTGAGGA GCGGCGCCAA ACCCTCCTGC AGAAGATCAG CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002695725.1
CDS	184..3363
Translation	MDLGPLNICEEMTILHGGFLLAEQLFRPKALAELTKSDWVHVGRPIVEALREISSTTACSQPFAWKKKALIIIWAKVLQPYPITPSDMETRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTVCRAELEHFLEHMTIDTSSKDVAFFLDVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDDFSHPPKRFKSDPDVCPTMPLLAMLLTGLKQIQDRILCPGMKCCALANLADMLTVFALMEEDPQEVSATVYLDKLATVISVWNSDTQNPYHQQALAEKVKEAERDISLTSLARLPSETIFVGFEFLRSLLQEWGEELQALLSSSQGTSYDSFRLCDSLTSFSQNLKLYLDTTSLAKEERQVVSELAECVGDFLRKTNRVVRSKGGEQDITASIAMAIIEQKMDRHMEMCYIFASERKWAFSDEWLSCLLNNRALFREPGLVLKLLETVMEVSTIDRAVSEPQIKQVFDLILECYTHLSLPDKNKVLSGVLLSWGRKGLSEKLLAHLEGFQEDLNTTFNQLTQSTSEQGLAKAVASVARLVILHPEITVKKLCSMAVVNLGTHKFLAQILTAFPALRFMEEQGPDRSTTFVVSCLKETVWTKLSTPREEKQFLELLSCLVSPTKPQGIPVAALLEPDEVLKEFVLPFLMLEVKEVDLSLRIFVQTLEGSTNLEEYWLQSCSPFPLIFSLCQLLDGFSKYWQLPKEKRCLPLDGKDLAIHILELLCEVVSANTDTFSPDTWVKSLSWLHRKLEQLDWTVGLRLKSFFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECSSISSSVSEQMLALLVVDVGNPEEVRLFSKGFLVALVQVMPWCSPQEWQCLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQLSVLLLRVFQFLCSQSCRNWLPVEGWSHVVKLLCNSLTNLLDSVRLIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMVHQEVCEPLYVLALEILTCYETLSKTNPSVSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_002695679.4
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002695679.4

ORF Insert Sequence:

ATGGACCTAG GACCTTTGAA TATCTGTGAG GAAATGACTA TCTTGCACGG GGGCTTCTTG 
CTGGCTGAGC AGCTGTTCCG CCCCAAAGCG CTGGCCGAAC TGACCAAGTC TGACTGGGTG 
CACGTTGGGC GGCCCATTGT GGAGGCTCTG AGGGAGATCT CCTCCACCAC AGCCTGCTCC 
CAGCCCTTCG CCTGGAAGAA GAAAGCGCTG ATCATCATCT GGGCCAAGGT GCTACAGCCC 
TACCCCATCA CCCCTTCCGA CATGGAAACC CGCTGGCAGG AAGATGTGTT CTTCTCTGTG 
GGCAACATGA TCCCCACCAT CAATCACACA GTCCTCTTTG AGCTGCTCAA GTCGCTGGAG 
GCCTCTGGAC TCTTTATCCA GCTCCTGATG GCCCTGCCCA CCACTGTCTG CCGTGCGGAA 
CTAGAGCACT TTTTGGAGCA CATGACCATC GACACCTCTT CCAAGGACGT GGCCTTCTTC 
CTCGACGTCT GGTGGGAAAT GATGAAGCAC AAGGGCAATC AGCAGGATCC CCTGCTCTCC 
CAGTTCCGGA CAATGGCCCA CAAGTACCTG TCCTCCTCCG ATGACTTCTC CCACCCGCCG 
AAGAGGTTCA AGTCCGATCC AGATGTGTGT CCAACCATGC CCCTGCTGGC CATGCTGCTC 
ACGGGGCTGA AACAAATCCA GGACAGGATC CTATGCCCCG GGATGAAGTG CTGCGCCTTA 
GCCAACTTGG CTGACATGCT GACCGTGTTT GCGCTGATGG AGGAGGACCC CCAGGAAGTG 
TCTGCGACCG TGTACCTGGA CAAGCTGGCC ACGGTGATCT CCGTGTGGAA CTCGGACACC 
CAGAACCCGT ATCACCAGCA GGCGCTGGCA GAGAAGGTGA AGGAGGCGGA GCGGGACATC 
AGCCTGACCT CCCTGGCCAG GCTGCCGAGC GAGACCATCT TCGTGGGCTT TGAGTTCCTG 
CGCAGCCTGC TGCAGGAGTG GGGGGAGGAG CTGCAGGCCT TGCTCAGCAG CAGCCAGGGG 
ACCAGCTACG ACAGCTTCCG GCTCTGCGAC AGCCTGACCT CCTTCAGCCA GAATCTGAAG 
CTCTACCTGG ACACCACCAG CCTGGCCAAG GAGGAGAGGC AGGTGGTCTC CGAGCTGGCA 
GAGTGCGTGG GGGACTTCCT GAGGAAGACG AACAGGGTGG TGAGGAGCAA GGGCGGTGAG 
CAGGACATCA CCGCCTCCAT CGCCATGGCC ATCATCGAGC AGAAGATGGA CCGCCACATG 
GAAATGTGCT ACATTTTTGC CTCCGAGAGG AAGTGGGCCT TCTCGGACGA GTGGCTGTCC 
TGCCTGCTCA ACAACCGAGC TCTCTTCCGA GAGCCGGGCC TGGTGTTAAA GCTGCTGGAG 
ACGGTGATGG AAGTCAGCAC GATAGACAGG GCTGTCTCCG AACCTCAGAT CAAACAGGTG 
TTCGACTTGA TCCTGGAGTG TTACACACAC CTCTCTCTGC CAGATAAAAA TAAAGTCCTC 
TCGGGGGTCC TGCTCTCCTG GGGGCGCAAG GGCCTCTCTG AGAAATTGTT AGCTCACTTG 
GAGGGCTTTC AGGAGGACCT CAACACGACT TTCAACCAGC TCACACAGAG CACCTCTGAA 
CAGGGCTTGG CTAAGGCTGT TGCTTCCGTG GCCCGCCTGG TCATCCTGCA CCCAGAGATC 
ACGGTGAAGA AGTTGTGCAG CATGGCTGTG GTCAACCTCG GCACCCACAA GTTCCTGGCT 
CAGATTCTCA CTGCCTTCCC CGCTCTCCGG TTCATGGAAG AGCAGGGGCC AGACCGCTCT 
ACCACATTCG TGGTGTCCTG CCTCAAAGAA ACGGTCTGGA CCAAGCTCTC GACACCCCGG 
GAGGAGAAGC AGTTTCTGGA GCTCTTGAGC TGCCTGGTGA GTCCTACAAA GCCCCAAGGG 
ATTCCAGTCG CTGCTCTGCT CGAGCCGGAT GAGGTACTAA AGGAGTTCGT CCTGCCTTTC 
TTGATGCTGG AGGTCAAAGA GGTGGACCTC AGTCTGAGGA TCTTCGTCCA GACTCTGGAA 
GGGAGCACAA ACCTGGAAGA ATACTGGCTG CAGTCCTGCT CTCCGTTCCC GCTCATCTTC 
AGCCTGTGCC AGCTCCTTGA TGGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAGCGC 
TGTCTCCCCC TGGACGGGAA GGACCTGGCC ATCCACATCC TGGAGCTCCT CTGTGAGGTG 
GTCTCAGCTA ACACCGACAC CTTCTCCCCA GACACCTGGG TCAAGTCGCT GTCCTGGCTC 
CACCGGAAGC TGGAGCAGCT AGACTGGACT GTGGGCCTGA GACTGAAGAG CTTCTTTGAG 
GGCCACTTCA AGTGCGAGGT GCCAGCCACG CTCTTTGAGA TCTGTAAGCT TTCTGAGGAC 
GAGTGGACCT CCCAGGCCCA CCCCGGGTAT GGGCCTGGCA CAGGCCTCCT GGCCTGGATG 
GAGTGCTCCT CCATCTCCAG CAGCGTCTCC GAGCAGATGC TCGCACTCCT GGTGGTGGAC 
GTGGGCAACC CTGAGGAGGT CAGGTTGTTC AGCAAGGGCT TCCTGGTGGC CCTGGTACAG 
GTCATGCCAT GGTGCAGCCC CCAGGAATGG CAGTGCCTTC ACCAGCTGAC CCGGAGACTG 
TTGGAGAAGC AACTCCTGCA CGTCCCCTAC AGCCTGGAGT ATATACAGTT CGTTCCTCTG 
CTCAACCTGA AGCCCTTTGC CCAGGAGCTC CAGCTCTCTG TCCTCTTGCT TAGAGTTTTT 
CAGTTTCTCT GCAGCCAGAG CTGCCGTAAC TGGCTCCCTG TGGAGGGCTG GAGCCATGTG 
GTCAAGCTCC TCTGCAACAG TCTGACCAAT CTCCTGGACT CTGTTCGGTT GATACAGTCG 
GTTGGCCCTT GGGCTCAAGG ACAAGAGCAG GACCTGACCC AGGAAACCCT CTTTTTCTAT 
ACCCAGGTCT TCTGTCATGT TCTGCACATC ATGGCCATGG TCCACCAGGA GGTCTGTGAA 
CCTCTCTATG TCTTGGCCTT GGAGATCCTC ACCTGCTACG AAACCCTGAG CAAGACCAAC 
CCTTCCGTCA GCTCCTTGCT CCAGAAAGTA AACGAGCAGC GCTTCTTAAA GTCCATCGCC 
GAGAACATCA GCCCTGAGGA GCGGCGCCAA ACCCTCCTGC AGAAGATCAG CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_002695679.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002695725.1 Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.

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CloneID	OBa10038
Clone ID Related Accession (Same CDS sequence)	XM_585199.8 , XM_002695679.4 , XM_002695679.5
Accession Version	XM_002695679.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	gem-associated protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002695679.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTAG GACCTTTGAA TATCTGTGAG GAAATGACTA TCTTGCACGG GGGCTTCTTG 
CTGGCTGAGC AGCTGTTCCG CCCCAAAGCG CTGGCCGAAC TGACCAAGTC TGACTGGGTG 
CACGTTGGGC GGCCCATTGT GGAGGCTCTG AGGGAGATCT CCTCCACCAC AGCCTGCTCC 
CAGCCCTTCG CCTGGAAGAA GAAAGCGCTG ATCATCATCT GGGCCAAGGT GCTACAGCCC 
TACCCCATCA CCCCTTCCGA CATGGAAACC CGCTGGCAGG AAGATGTGTT CTTCTCTGTG 
GGCAACATGA TCCCCACCAT CAATCACACA GTCCTCTTTG AGCTGCTCAA GTCGCTGGAG 
GCCTCTGGAC TCTTTATCCA GCTCCTGATG GCCCTGCCCA CCACTGTCTG CCGTGCGGAA 
CTAGAGCACT TTTTGGAGCA CATGACCATC GACACCTCTT CCAAGGACGT GGCCTTCTTC 
CTCGACGTCT GGTGGGAAAT GATGAAGCAC AAGGGCAATC AGCAGGATCC CCTGCTCTCC 
CAGTTCCGGA CAATGGCCCA CAAGTACCTG TCCTCCTCCG ATGACTTCTC CCACCCGCCG 
AAGAGGTTCA AGTCCGATCC AGATGTGTGT CCAACCATGC CCCTGCTGGC CATGCTGCTC 
ACGGGGCTGA AACAAATCCA GGACAGGATC CTATGCCCCG GGATGAAGTG CTGCGCCTTA 
GCCAACTTGG CTGACATGCT GACCGTGTTT GCGCTGATGG AGGAGGACCC CCAGGAAGTG 
TCTGCGACCG TGTACCTGGA CAAGCTGGCC ACGGTGATCT CCGTGTGGAA CTCGGACACC 
CAGAACCCGT ATCACCAGCA GGCGCTGGCA GAGAAGGTGA AGGAGGCGGA GCGGGACATC 
AGCCTGACCT CCCTGGCCAG GCTGCCGAGC GAGACCATCT TCGTGGGCTT TGAGTTCCTG 
CGCAGCCTGC TGCAGGAGTG GGGGGAGGAG CTGCAGGCCT TGCTCAGCAG CAGCCAGGGG 
ACCAGCTACG ACAGCTTCCG GCTCTGCGAC AGCCTGACCT CCTTCAGCCA GAATCTGAAG 
CTCTACCTGG ACACCACCAG CCTGGCCAAG GAGGAGAGGC AGGTGGTCTC CGAGCTGGCA 
GAGTGCGTGG GGGACTTCCT GAGGAAGACG AACAGGGTGG TGAGGAGCAA GGGCGGTGAG 
CAGGACATCA CCGCCTCCAT CGCCATGGCC ATCATCGAGC AGAAGATGGA CCGCCACATG 
GAAATGTGCT ACATTTTTGC CTCCGAGAGG AAGTGGGCCT TCTCGGACGA GTGGCTGTCC 
TGCCTGCTCA ACAACCGAGC TCTCTTCCGA GAGCCGGGCC TGGTGTTAAA GCTGCTGGAG 
ACGGTGATGG AAGTCAGCAC GATAGACAGG GCTGTCTCCG AACCTCAGAT CAAACAGGTG 
TTCGACTTGA TCCTGGAGTG TTACACACAC CTCTCTCTGC CAGATAAAAA TAAAGTCCTC 
TCGGGGGTCC TGCTCTCCTG GGGGCGCAAG GGCCTCTCTG AGAAATTGTT AGCTCACTTG 
GAGGGCTTTC AGGAGGACCT CAACACGACT TTCAACCAGC TCACACAGAG CACCTCTGAA 
CAGGGCTTGG CTAAGGCTGT TGCTTCCGTG GCCCGCCTGG TCATCCTGCA CCCAGAGATC 
ACGGTGAAGA AGTTGTGCAG CATGGCTGTG GTCAACCTCG GCACCCACAA GTTCCTGGCT 
CAGATTCTCA CTGCCTTCCC CGCTCTCCGG TTCATGGAAG AGCAGGGGCC AGACCGCTCT 
ACCACATTCG TGGTGTCCTG CCTCAAAGAA ACGGTCTGGA CCAAGCTCTC GACACCCCGG 
GAGGAGAAGC AGTTTCTGGA GCTCTTGAGC TGCCTGGTGA GTCCTACAAA GCCCCAAGGG 
ATTCCAGTCG CTGCTCTGCT CGAGCCGGAT GAGGTACTAA AGGAGTTCGT CCTGCCTTTC 
TTGATGCTGG AGGTCAAAGA GGTGGACCTC AGTCTGAGGA TCTTCGTCCA GACTCTGGAA 
GGGAGCACAA ACCTGGAAGA ATACTGGCTG CAGTCCTGCT CTCCGTTCCC GCTCATCTTC 
AGCCTGTGCC AGCTCCTTGA TGGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAGCGC 
TGTCTCCCCC TGGACGGGAA GGACCTGGCC ATCCACATCC TGGAGCTCCT CTGTGAGGTG 
GTCTCAGCTA ACACCGACAC CTTCTCCCCA GACACCTGGG TCAAGTCGCT GTCCTGGCTC 
CACCGGAAGC TGGAGCAGCT AGACTGGACT GTGGGCCTGA GACTGAAGAG CTTCTTTGAG 
GGCCACTTCA AGTGCGAGGT GCCAGCCACG CTCTTTGAGA TCTGTAAGCT TTCTGAGGAC 
GAGTGGACCT CCCAGGCCCA CCCCGGGTAT GGGCCTGGCA CAGGCCTCCT GGCCTGGATG 
GAGTGCTCCT CCATCTCCAG CAGCGTCTCC GAGCAGATGC TCGCACTCCT GGTGGTGGAC 
GTGGGCAACC CTGAGGAGGT CAGGTTGTTC AGCAAGGGCT TCCTGGTGGC CCTGGTACAG 
GTCATGCCAT GGTGCAGCCC CCAGGAATGG CAGTGCCTTC ACCAGCTGAC CCGGAGACTG 
TTGGAGAAGC AACTCCTGCA CGTCCCCTAC AGCCTGGAGT ATATACAGTT CGTTCCTCTG 
CTCAACCTGA AGCCCTTTGC CCAGGAGCTC CAGCTCTCTG TCCTCTTGCT TAGAGTTTTT 
CAGTTTCTCT GCAGCCAGAG CTGCCGTAAC TGGCTCCCTG TGGAGGGCTG GAGCCATGTG 
GTCAAGCTCC TCTGCAACAG TCTGACCAAT CTCCTGGACT CTGTTCGGTT GATACAGTCG 
GTTGGCCCTT GGGCTCAAGG ACAAGAGCAG GACCTGACCC AGGAAACCCT CTTTTTCTAT 
ACCCAGGTCT TCTGTCATGT TCTGCACATC ATGGCCATGG TCCACCAGGA GGTCTGTGAA 
CCTCTCTATG TCTTGGCCTT GGAGATCCTC ACCTGCTACG AAACCCTGAG CAAGACCAAC 
CCTTCCGTCA GCTCCTTGCT CCAGAAAGTA AACGAGCAGC GCTTCTTAAA GTCCATCGCC 
GAGAACATCA GCCCTGAGGA GCGGCGCCAA ACCCTCCTGC AGAAGATCAG CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002695725.1
CDS	179..3358
Translation	MDLGPLNICEEMTILHGGFLLAEQLFRPKALAELTKSDWVHVGRPIVEALREISSTTACSQPFAWKKKALIIIWAKVLQPYPITPSDMETRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTVCRAELEHFLEHMTIDTSSKDVAFFLDVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDDFSHPPKRFKSDPDVCPTMPLLAMLLTGLKQIQDRILCPGMKCCALANLADMLTVFALMEEDPQEVSATVYLDKLATVISVWNSDTQNPYHQQALAEKVKEAERDISLTSLARLPSETIFVGFEFLRSLLQEWGEELQALLSSSQGTSYDSFRLCDSLTSFSQNLKLYLDTTSLAKEERQVVSELAECVGDFLRKTNRVVRSKGGEQDITASIAMAIIEQKMDRHMEMCYIFASERKWAFSDEWLSCLLNNRALFREPGLVLKLLETVMEVSTIDRAVSEPQIKQVFDLILECYTHLSLPDKNKVLSGVLLSWGRKGLSEKLLAHLEGFQEDLNTTFNQLTQSTSEQGLAKAVASVARLVILHPEITVKKLCSMAVVNLGTHKFLAQILTAFPALRFMEEQGPDRSTTFVVSCLKETVWTKLSTPREEKQFLELLSCLVSPTKPQGIPVAALLEPDEVLKEFVLPFLMLEVKEVDLSLRIFVQTLEGSTNLEEYWLQSCSPFPLIFSLCQLLDGFSKYWQLPKEKRCLPLDGKDLAIHILELLCEVVSANTDTFSPDTWVKSLSWLHRKLEQLDWTVGLRLKSFFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECSSISSSVSEQMLALLVVDVGNPEEVRLFSKGFLVALVQVMPWCSPQEWQCLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQLSVLLLRVFQFLCSQSCRNWLPVEGWSHVVKLLCNSLTNLLDSVRLIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMVHQEVCEPLYVLALEILTCYETLSKTNPSVSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_002695679.5
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002695679.5

ORF Insert Sequence:

ATGGACCTAG GACCTTTGAA TATCTGTGAG GAAATGACTA TCTTGCACGG GGGCTTCTTG 
CTGGCTGAGC AGCTGTTCCG CCCCAAAGCG CTGGCCGAAC TGACCAAGTC TGACTGGGTG 
CACGTTGGGC GGCCCATTGT GGAGGCTCTG AGGGAGATCT CCTCCACCAC AGCCTGCTCC 
CAGCCCTTCG CCTGGAAGAA GAAAGCGCTG ATCATCATCT GGGCCAAGGT GCTACAGCCC 
TACCCCATCA CCCCTTCCGA CATGGAAACC CGCTGGCAGG AAGATGTGTT CTTCTCTGTG 
GGCAACATGA TCCCCACCAT CAATCACACA GTCCTCTTTG AGCTGCTCAA GTCGCTGGAG 
GCCTCTGGAC TCTTTATCCA GCTCCTGATG GCCCTGCCCA CCACTGTCTG CCGTGCGGAA 
CTAGAGCACT TTTTGGAGCA CATGACCATC GACACCTCTT CCAAGGACGT GGCCTTCTTC 
CTCGACGTCT GGTGGGAAAT GATGAAGCAC AAGGGCAATC AGCAGGATCC CCTGCTCTCC 
CAGTTCCGGA CAATGGCCCA CAAGTACCTG TCCTCCTCCG ATGACTTCTC CCACCCGCCG 
AAGAGGTTCA AGTCCGATCC AGATGTGTGT CCAACCATGC CCCTGCTGGC CATGCTGCTC 
ACGGGGCTGA AACAAATCCA GGACAGGATC CTATGCCCCG GGATGAAGTG CTGCGCCTTA 
GCCAACTTGG CTGACATGCT GACCGTGTTT GCGCTGATGG AGGAGGACCC CCAGGAAGTG 
TCTGCGACCG TGTACCTGGA CAAGCTGGCC ACGGTGATCT CCGTGTGGAA CTCGGACACC 
CAGAACCCGT ATCACCAGCA GGCGCTGGCA GAGAAGGTGA AGGAGGCGGA GCGGGACATC 
AGCCTGACCT CCCTGGCCAG GCTGCCGAGC GAGACCATCT TCGTGGGCTT TGAGTTCCTG 
CGCAGCCTGC TGCAGGAGTG GGGGGAGGAG CTGCAGGCCT TGCTCAGCAG CAGCCAGGGG 
ACCAGCTACG ACAGCTTCCG GCTCTGCGAC AGCCTGACCT CCTTCAGCCA GAATCTGAAG 
CTCTACCTGG ACACCACCAG CCTGGCCAAG GAGGAGAGGC AGGTGGTCTC CGAGCTGGCA 
GAGTGCGTGG GGGACTTCCT GAGGAAGACG AACAGGGTGG TGAGGAGCAA GGGCGGTGAG 
CAGGACATCA CCGCCTCCAT CGCCATGGCC ATCATCGAGC AGAAGATGGA CCGCCACATG 
GAAATGTGCT ACATTTTTGC CTCCGAGAGG AAGTGGGCCT TCTCGGACGA GTGGCTGTCC 
TGCCTGCTCA ACAACCGAGC TCTCTTCCGA GAGCCGGGCC TGGTGTTAAA GCTGCTGGAG 
ACGGTGATGG AAGTCAGCAC GATAGACAGG GCTGTCTCCG AACCTCAGAT CAAACAGGTG 
TTCGACTTGA TCCTGGAGTG TTACACACAC CTCTCTCTGC CAGATAAAAA TAAAGTCCTC 
TCGGGGGTCC TGCTCTCCTG GGGGCGCAAG GGCCTCTCTG AGAAATTGTT AGCTCACTTG 
GAGGGCTTTC AGGAGGACCT CAACACGACT TTCAACCAGC TCACACAGAG CACCTCTGAA 
CAGGGCTTGG CTAAGGCTGT TGCTTCCGTG GCCCGCCTGG TCATCCTGCA CCCAGAGATC 
ACGGTGAAGA AGTTGTGCAG CATGGCTGTG GTCAACCTCG GCACCCACAA GTTCCTGGCT 
CAGATTCTCA CTGCCTTCCC CGCTCTCCGG TTCATGGAAG AGCAGGGGCC AGACCGCTCT 
ACCACATTCG TGGTGTCCTG CCTCAAAGAA ACGGTCTGGA CCAAGCTCTC GACACCCCGG 
GAGGAGAAGC AGTTTCTGGA GCTCTTGAGC TGCCTGGTGA GTCCTACAAA GCCCCAAGGG 
ATTCCAGTCG CTGCTCTGCT CGAGCCGGAT GAGGTACTAA AGGAGTTCGT CCTGCCTTTC 
TTGATGCTGG AGGTCAAAGA GGTGGACCTC AGTCTGAGGA TCTTCGTCCA GACTCTGGAA 
GGGAGCACAA ACCTGGAAGA ATACTGGCTG CAGTCCTGCT CTCCGTTCCC GCTCATCTTC 
AGCCTGTGCC AGCTCCTTGA TGGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAGCGC 
TGTCTCCCCC TGGACGGGAA GGACCTGGCC ATCCACATCC TGGAGCTCCT CTGTGAGGTG 
GTCTCAGCTA ACACCGACAC CTTCTCCCCA GACACCTGGG TCAAGTCGCT GTCCTGGCTC 
CACCGGAAGC TGGAGCAGCT AGACTGGACT GTGGGCCTGA GACTGAAGAG CTTCTTTGAG 
GGCCACTTCA AGTGCGAGGT GCCAGCCACG CTCTTTGAGA TCTGTAAGCT TTCTGAGGAC 
GAGTGGACCT CCCAGGCCCA CCCCGGGTAT GGGCCTGGCA CAGGCCTCCT GGCCTGGATG 
GAGTGCTCCT CCATCTCCAG CAGCGTCTCC GAGCAGATGC TCGCACTCCT GGTGGTGGAC 
GTGGGCAACC CTGAGGAGGT CAGGTTGTTC AGCAAGGGCT TCCTGGTGGC CCTGGTACAG 
GTCATGCCAT GGTGCAGCCC CCAGGAATGG CAGTGCCTTC ACCAGCTGAC CCGGAGACTG 
TTGGAGAAGC AACTCCTGCA CGTCCCCTAC AGCCTGGAGT ATATACAGTT CGTTCCTCTG 
CTCAACCTGA AGCCCTTTGC CCAGGAGCTC CAGCTCTCTG TCCTCTTGCT TAGAGTTTTT 
CAGTTTCTCT GCAGCCAGAG CTGCCGTAAC TGGCTCCCTG TGGAGGGCTG GAGCCATGTG 
GTCAAGCTCC TCTGCAACAG TCTGACCAAT CTCCTGGACT CTGTTCGGTT GATACAGTCG 
GTTGGCCCTT GGGCTCAAGG ACAAGAGCAG GACCTGACCC AGGAAACCCT CTTTTTCTAT 
ACCCAGGTCT TCTGTCATGT TCTGCACATC ATGGCCATGG TCCACCAGGA GGTCTGTGAA 
CCTCTCTATG TCTTGGCCTT GGAGATCCTC ACCTGCTACG AAACCCTGAG CAAGACCAAC 
CCTTCCGTCA GCTCCTTGCT CCAGAAAGTA AACGAGCAGC GCTTCTTAAA GTCCATCGCC 
GAGAACATCA GCCCTGAGGA GCGGCGCCAA ACCCTCCTGC AGAAGATCAG CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_010815869.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814171.1 Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa29565
Clone ID Related Accession (Same CDS sequence)	XM_010815869.2 , XM_010824016.2
Accession Version	XM_010815869.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	gem-associated protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815869.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGGAGTG GTGTGTCCAT 
GAAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT 
GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT 
GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC 
TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC 
ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC 
ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT 
GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG 
CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC 
GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC 
CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG 
TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG 
CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC 
TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG 
ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC 
CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG 
ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC 
CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC 
TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC 
CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC 
GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC 
ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG 
TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG 
CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG 
ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC 
TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG 
GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC 
TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC 
TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG 
AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT 
CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA 
TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG 
AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA 
GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG 
CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC 
ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG 
TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC 
CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA 
GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG 
AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC 
TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG 
ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC 
TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC 
AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG 
CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG 
AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC 
CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT 
CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG 
CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC 
CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG 
GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC 
TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC 
GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC 
ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814171.1
CDS	305..3538
Translation	MRGTGAPLRGRKALREWCVHEGPLNICEEMTILHGGFLLAEQLFRPKALAELTKSDWVHVGRPIVEALREISSTTACSQPFAWKKKALIIIWAKVLQPYPITPSDMETRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTVCRAELEHFLEHMTIDTSSKDVAFFLDVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDDFSHPPKRFKSDPDVCPTMPLLAMLLTGLKQIQDRILCPGMKCCALANLADMLTVFALMEEDPQEVSATVYLDKLATVISVWNSDTQNPYHQQALAEKVKEAERDISLTSLARLPSETIFVGFEFLRSLLQEWGEELQALLSSSQGTSYDSFRLCDSLTSFSQNLKLYLDTTSLAKEERQVVSELAECVGDFLRKTNRVVRSKGGEQDITASIAMAIIEQKMDRHMEMCYIFASERKWAFSDEWLSCLLNNRALFREPGLVLKLLETVMEVSTIDRAVSEPQIKQVFDLILECYTHLSLPDKNKVLSGVLLSWGRKGLSEKLLAHLEGFQEDLNTTFNQLTQSTSEQGLAKAVASVARLVILHPEITVKKLCSMAVVNLGTHKFLAQILTAFPALRFMEEQGPDRSTTFVVSCLKETVWTKLSTPREEKQFLELLSCLVSPTKPQGIPVAALLEPDEVLKEFVLPFLMLEVKEVDLSLRIFVQTLEGSTNLEEYWLQSCSPFPLIFSLCQLLDGFSKYWQLPKEKRCLPLDGKDLAIHILELLCEVVSANTDTFSPDTWVKSLSWLHRKLEQLDWTVGLRLKSFFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECSSISSSVSEQMLALLVVDVGNPEEVRLFSKGFLVALVQVMPWCSPQEWQCLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQLSVLLLRVFQFLCSQSCRNWLPVEGWSHVVKLLCNSLTNLLDSVRLIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMVHQEVCEPLYVLALEILTCYETLSKTNPSVSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_010815869.2
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815869.2

ORF Insert Sequence:

ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGGAGTG GTGTGTCCAT 
GAAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT 
GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT 
GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC 
TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC 
ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC 
ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT 
GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG 
CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC 
GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC 
CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG 
TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG 
CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC 
TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG 
ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC 
CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG 
ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC 
CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC 
TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC 
CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC 
GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC 
ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG 
TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG 
CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG 
ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC 
TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG 
GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC 
TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC 
TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG 
AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT 
CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA 
TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG 
AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA 
GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG 
CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC 
ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG 
TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC 
CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA 
GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG 
AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC 
TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG 
ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC 
TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC 
AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG 
CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG 
AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC 
CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT 
CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG 
CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC 
CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG 
GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC 
TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC 
GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC 
ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_010824016.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822318.2 Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa29565
Clone ID Related Accession (Same CDS sequence)	XM_010815869.2 , XM_010824016.2
Accession Version	XM_010824016.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	gem-associated protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGGAGTG GTGTGTCCAT 
GAAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT 
GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT 
GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC 
TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC 
ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC 
ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT 
GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG 
CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC 
GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC 
CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG 
TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG 
CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC 
TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG 
ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC 
CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG 
ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC 
CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC 
TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC 
CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC 
GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC 
ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG 
TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG 
CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG 
ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC 
TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG 
GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC 
TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC 
TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG 
AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT 
CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA 
TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG 
AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA 
GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG 
CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC 
ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG 
TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC 
CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA 
GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG 
AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC 
TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG 
ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC 
TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC 
AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG 
CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG 
AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC 
CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT 
CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG 
CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC 
CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG 
GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC 
TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC 
GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC 
ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822318.2
CDS	305..3538
Translation	MRGTGAPLRGRKALREWCVHEGPLNICEEMTILHGGFLLAEQLFRPKALAELTKSDWVHVGRPIVEALREISSTTACSQPFAWKKKALIIIWAKVLQPYPITPSDMETRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTVCRAELEHFLEHMTIDTSSKDVAFFLDVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDDFSHPPKRFKSDPDVCPTMPLLAMLLTGLKQIQDRILCPGMKCCALANLADMLTVFALMEEDPQEVSATVYLDKLATVISVWNSDTQNPYHQQALAEKVKEAERDISLTSLARLPSETIFVGFEFLRSLLQEWGEELQALLSSSQGTSYDSFRLCDSLTSFSQNLKLYLDTTSLAKEERQVVSELAECVGDFLRKTNRVVRSKGGEQDITASIAMAIIEQKMDRHMEMCYIFASERKWAFSDEWLSCLLNNRALFREPGLVLKLLETVMEVSTIDRAVSEPQIKQVFDLILECYTHLSLPDKNKVLSGVLLSWGRKGLSEKLLAHLEGFQEDLNTTFNQLTQSTSEQGLAKAVASVARLVILHPEITVKKLCSMAVVNLGTHKFLAQILTAFPALRFMEEQGPDRSTTFVVSCLKETVWTKLSTPREEKQFLELLSCLVSPTKPQGIPVAALLEPDEVLKEFVLPFLMLEVKEVDLSLRIFVQTLEGSTNLEEYWLQSCSPFPLIFSLCQLLDGFSKYWQLPKEKRCLPLDGKDLAIHILELLCEVVSANTDTFSPDTWVKSLSWLHRKLEQLDWTVGLRLKSFFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECSSISSSVSEQMLALLVVDVGNPEEVRLFSKGFLVALVQVMPWCSPQEWQCLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQLSVLLLRVFQFLCSQSCRNWLPVEGWSHVVKLLCNSLTNLLDSVRLIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMVHQEVCEPLYVLALEILTCYETLSKTNPSVSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_010824016.2
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824016.2

ORF Insert Sequence:

ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGGAGTG GTGTGTCCAT 
GAAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT 
GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT 
GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC 
TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC 
ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC 
ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT 
GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG 
CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC 
GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC 
CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG 
TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG 
CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC 
TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG 
ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC 
CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG 
ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC 
CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC 
TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC 
CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC 
GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC 
ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG 
TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG 
CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG 
ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC 
TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG 
GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC 
TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC 
TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG 
AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT 
CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA 
TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG 
AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA 
GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG 
CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC 
ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG 
TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC 
CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA 
GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG 
AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC 
TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG 
ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC 
TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC 
AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG 
CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG 
AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC 
CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT 
CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG 
CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC 
CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG 
GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC 
TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC 
GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC 
ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_010815870.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814172.1 Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa54209
Clone ID Related Accession (Same CDS sequence)	XM_010815870.2 , XM_010824017.2
Accession Version	XM_010815870.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	gem-associated protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTATCT TGCACGGGGG CTTCTTGCTG GCTGAGCAGC TGTTCCGCCC CAAAGCGCTG 
GCCGAACTGA CCAAGTCTGA CTGGGTGCAC GTTGGGCGGC CCATTGTGGA GGCTCTGAGG 
GAGATCTCCT CCACCACAGC CTGCTCCCAG CCCTTCGCCT GGAAGAAGAA AGCGCTGATC 
ATCATCTGGG CCAAGGTGCT ACAGCCCTAC CCCATCACCC CTTCCGACAT GGAAACCCGC 
TGGCAGGAAG ATGTGTTCTT CTCTGTGGGC AACATGATCC CCACCATCAA TCACACAGTC 
CTCTTTGAGC TGCTCAAGTC GCTGGAGGCC TCTGGACTCT TTATCCAGCT CCTGATGGCC 
CTGCCCACCA CTGTCTGCCG TGCGGAACTA GAGCACTTTT TGGAGCACAT GACCATCGAC 
ACCTCTTCCA AGGACGTGGC CTTCTTCCTC GACGTCTGGT GGGAAATGAT GAAGCACAAG 
GGCAATCAGC AGGATCCCCT GCTCTCCCAG TTCCGGACAA TGGCCCACAA GTACCTGTCC 
TCCTCCGATG ACTTCTCCCA CCCGCCGAAG AGGTTCAAGT CCGATCCAGA TGTGTGTCCA 
ACCATGCCCC TGCTGGCCAT GCTGCTCACG GGGCTGAAAC AAATCCAGGA CAGGATCCTA 
TGCCCCGGGA TGAAGTGCTG CGCCTTAGCC AACTTGGCTG ACATGCTGAC CGTGTTTGCG 
CTGATGGAGG AGGACCCCCA GGAAGTGTCT GCGACCGTGT ACCTGGACAA GCTGGCCACG 
GTGATCTCCG TGTGGAACTC GGACACCCAG AACCCGTATC ACCAGCAGGC GCTGGCAGAG 
AAGGTGAAGG AGGCGGAGCG GGACATCAGC CTGACCTCCC TGGCCAGGCT GCCGAGCGAG 
ACCATCTTCG TGGGCTTTGA GTTCCTGCGC AGCCTGCTGC AGGAGTGGGG GGAGGAGCTG 
CAGGCCTTGC TCAGCAGCAG CCAGGGGACC AGCTACGACA GCTTCCGGCT CTGCGACAGC 
CTGACCTCCT TCAGCCAGAA TCTGAAGCTC TACCTGGACA CCACCAGCCT GGCCAAGGAG 
GAGAGGCAGG TGGTCTCCGA GCTGGCAGAG TGCGTGGGGG ACTTCCTGAG GAAGACGAAC 
AGGGTGGTGA GGAGCAAGGG CGGTGAGCAG GACATCACCG CCTCCATCGC CATGGCCATC 
ATCGAGCAGA AGATGGACCG CCACATGGAA ATGTGCTACA TTTTTGCCTC CGAGAGGAAG 
TGGGCCTTCT CGGACGAGTG GCTGTCCTGC CTGCTCAACA ACCGAGCTCT CTTCCGAGAG 
CCGGGCCTGG TGTTAAAGCT GCTGGAGACG GTGATGGAAG TCAGCACGAT AGACAGGGCT 
GTCTCCGAAC CTCAGATCAA ACAGGTGTTC GACTTGATCC TGGAGTGTTA CACACACCTC 
TCTCTGCCAG ATAAAAATAA AGTCCTCTCG GGGGTCCTGC TCTCCTGGGG GCGCAAGGGC 
CTCTCTGAGA AATTGTTAGC TCACTTGGAG GGCTTTCAGG AGGACCTCAA CACGACTTTC 
AACCAGCTCA CACAGAGCAC CTCTGAACAG GGCTTGGCTA AGGCTGTTGC TTCCGTGGCC 
CGCCTGGTCA TCCTGCACCC AGAGATCACG GTGAAGAAGT TGTGCAGCAT GGCTGTGGTC 
AACCTCGGCA CCCACAAGTT CCTGGCTCAG ATTCTCACTG CCTTCCCCGC TCTCCGGTTC 
ATGGAAGAGC AGGGGCCAGA CCGCTCTACC ACATTCGTGG TGTCCTGCCT CAAAGAAACG 
GTCTGGACCA AGCTCTCGAC ACCCCGGGAG GAGAAGCAGT TTCTGGAGCT CTTGAGCTGC 
CTGGTGAGTC CTACAAAGCC CCAAGGGATT CCAGTCGCTG CTCTGCTCGA GCCGGATGAG 
GTACTAAAGG AGTTCGTCCT GCCTTTCTTG ATGCTGGAGG TCAAAGAGGT GGACCTCAGT 
CTGAGGATCT TCGTCCAGAC TCTGGAAGGG AGCACAAACC TGGAAGAATA CTGGCTGCAG 
TCCTGCTCTC CGTTCCCGCT CATCTTCAGC CTGTGCCAGC TCCTTGATGG CTTCAGCAAA 
TACTGGCAGC TCCCCAAGGA GAAGCGCTGT CTCCCCCTGG ACGGGAAGGA CCTGGCCATC 
CACATCCTGG AGCTCCTCTG TGAGGTGGTC TCAGCTAACA CCGACACCTT CTCCCCAGAC 
ACCTGGGTCA AGTCGCTGTC CTGGCTCCAC CGGAAGCTGG AGCAGCTAGA CTGGACTGTG 
GGCCTGAGAC TGAAGAGCTT CTTTGAGGGC CACTTCAAGT GCGAGGTGCC AGCCACGCTC 
TTTGAGATCT GTAAGCTTTC TGAGGACGAG TGGACCTCCC AGGCCCACCC CGGGTATGGG 
CCTGGCACAG GCCTCCTGGC CTGGATGGAG TGCTCCTCCA TCTCCAGCAG CGTCTCCGAG 
CAGATGCTCG CACTCCTGGT GGTGGACGTG GGCAACCCTG AGGAGGTCAG GTTGTTCAGC 
AAGGGCTTCC TGGTGGCCCT GGTACAGGTC ATGCCATGGT GCAGCCCCCA GGAATGGCAG 
TGCCTTCACC AGCTGACCCG GAGACTGTTG GAGAAGCAAC TCCTGCACGT CCCCTACAGC 
CTGGAGTATA TACAGTTCGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCA GGAGCTCCAG 
CTCTCTGTCC TCTTGCTTAG AGTTTTTCAG TTTCTCTGCA GCCAGAGCTG CCGTAACTGG 
CTCCCTGTGG AGGGCTGGAG CCATGTGGTC AAGCTCCTCT GCAACAGTCT GACCAATCTC 
CTGGACTCTG TTCGGTTGAT ACAGTCGGTT GGCCCTTGGG CTCAAGGACA AGAGCAGGAC 
CTGACCCAGG AAACCCTCTT TTTCTATACC CAGGTCTTCT GTCATGTTCT GCACATCATG 
GCCATGGTCC ACCAGGAGGT CTGTGAACCT CTCTATGTCT TGGCCTTGGA GATCCTCACC 
TGCTACGAAA CCCTGAGCAA GACCAACCCT TCCGTCAGCT CCTTGCTCCA GAAAGTAAAC 
GAGCAGCGCT TCTTAAAGTC CATCGCCGAG AACATCAGCC CTGAGGAGCG GCGCCAAACC 
CTCCTGCAGA AGATCAGCAA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814172.1
CDS	191..3337
Translation	MTILHGGFLLAEQLFRPKALAELTKSDWVHVGRPIVEALREISSTTACSQPFAWKKKALIIIWAKVLQPYPITPSDMETRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTVCRAELEHFLEHMTIDTSSKDVAFFLDVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDDFSHPPKRFKSDPDVCPTMPLLAMLLTGLKQIQDRILCPGMKCCALANLADMLTVFALMEEDPQEVSATVYLDKLATVISVWNSDTQNPYHQQALAEKVKEAERDISLTSLARLPSETIFVGFEFLRSLLQEWGEELQALLSSSQGTSYDSFRLCDSLTSFSQNLKLYLDTTSLAKEERQVVSELAECVGDFLRKTNRVVRSKGGEQDITASIAMAIIEQKMDRHMEMCYIFASERKWAFSDEWLSCLLNNRALFREPGLVLKLLETVMEVSTIDRAVSEPQIKQVFDLILECYTHLSLPDKNKVLSGVLLSWGRKGLSEKLLAHLEGFQEDLNTTFNQLTQSTSEQGLAKAVASVARLVILHPEITVKKLCSMAVVNLGTHKFLAQILTAFPALRFMEEQGPDRSTTFVVSCLKETVWTKLSTPREEKQFLELLSCLVSPTKPQGIPVAALLEPDEVLKEFVLPFLMLEVKEVDLSLRIFVQTLEGSTNLEEYWLQSCSPFPLIFSLCQLLDGFSKYWQLPKEKRCLPLDGKDLAIHILELLCEVVSANTDTFSPDTWVKSLSWLHRKLEQLDWTVGLRLKSFFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECSSISSSVSEQMLALLVVDVGNPEEVRLFSKGFLVALVQVMPWCSPQEWQCLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQLSVLLLRVFQFLCSQSCRNWLPVEGWSHVVKLLCNSLTNLLDSVRLIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMVHQEVCEPLYVLALEILTCYETLSKTNPSVSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_010815870.2
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815870.2

ORF Insert Sequence:

ATGACTATCT TGCACGGGGG CTTCTTGCTG GCTGAGCAGC TGTTCCGCCC CAAAGCGCTG 
GCCGAACTGA CCAAGTCTGA CTGGGTGCAC GTTGGGCGGC CCATTGTGGA GGCTCTGAGG 
GAGATCTCCT CCACCACAGC CTGCTCCCAG CCCTTCGCCT GGAAGAAGAA AGCGCTGATC 
ATCATCTGGG CCAAGGTGCT ACAGCCCTAC CCCATCACCC CTTCCGACAT GGAAACCCGC 
TGGCAGGAAG ATGTGTTCTT CTCTGTGGGC AACATGATCC CCACCATCAA TCACACAGTC 
CTCTTTGAGC TGCTCAAGTC GCTGGAGGCC TCTGGACTCT TTATCCAGCT CCTGATGGCC 
CTGCCCACCA CTGTCTGCCG TGCGGAACTA GAGCACTTTT TGGAGCACAT GACCATCGAC 
ACCTCTTCCA AGGACGTGGC CTTCTTCCTC GACGTCTGGT GGGAAATGAT GAAGCACAAG 
GGCAATCAGC AGGATCCCCT GCTCTCCCAG TTCCGGACAA TGGCCCACAA GTACCTGTCC 
TCCTCCGATG ACTTCTCCCA CCCGCCGAAG AGGTTCAAGT CCGATCCAGA TGTGTGTCCA 
ACCATGCCCC TGCTGGCCAT GCTGCTCACG GGGCTGAAAC AAATCCAGGA CAGGATCCTA 
TGCCCCGGGA TGAAGTGCTG CGCCTTAGCC AACTTGGCTG ACATGCTGAC CGTGTTTGCG 
CTGATGGAGG AGGACCCCCA GGAAGTGTCT GCGACCGTGT ACCTGGACAA GCTGGCCACG 
GTGATCTCCG TGTGGAACTC GGACACCCAG AACCCGTATC ACCAGCAGGC GCTGGCAGAG 
AAGGTGAAGG AGGCGGAGCG GGACATCAGC CTGACCTCCC TGGCCAGGCT GCCGAGCGAG 
ACCATCTTCG TGGGCTTTGA GTTCCTGCGC AGCCTGCTGC AGGAGTGGGG GGAGGAGCTG 
CAGGCCTTGC TCAGCAGCAG CCAGGGGACC AGCTACGACA GCTTCCGGCT CTGCGACAGC 
CTGACCTCCT TCAGCCAGAA TCTGAAGCTC TACCTGGACA CCACCAGCCT GGCCAAGGAG 
GAGAGGCAGG TGGTCTCCGA GCTGGCAGAG TGCGTGGGGG ACTTCCTGAG GAAGACGAAC 
AGGGTGGTGA GGAGCAAGGG CGGTGAGCAG GACATCACCG CCTCCATCGC CATGGCCATC 
ATCGAGCAGA AGATGGACCG CCACATGGAA ATGTGCTACA TTTTTGCCTC CGAGAGGAAG 
TGGGCCTTCT CGGACGAGTG GCTGTCCTGC CTGCTCAACA ACCGAGCTCT CTTCCGAGAG 
CCGGGCCTGG TGTTAAAGCT GCTGGAGACG GTGATGGAAG TCAGCACGAT AGACAGGGCT 
GTCTCCGAAC CTCAGATCAA ACAGGTGTTC GACTTGATCC TGGAGTGTTA CACACACCTC 
TCTCTGCCAG ATAAAAATAA AGTCCTCTCG GGGGTCCTGC TCTCCTGGGG GCGCAAGGGC 
CTCTCTGAGA AATTGTTAGC TCACTTGGAG GGCTTTCAGG AGGACCTCAA CACGACTTTC 
AACCAGCTCA CACAGAGCAC CTCTGAACAG GGCTTGGCTA AGGCTGTTGC TTCCGTGGCC 
CGCCTGGTCA TCCTGCACCC AGAGATCACG GTGAAGAAGT TGTGCAGCAT GGCTGTGGTC 
AACCTCGGCA CCCACAAGTT CCTGGCTCAG ATTCTCACTG CCTTCCCCGC TCTCCGGTTC 
ATGGAAGAGC AGGGGCCAGA CCGCTCTACC ACATTCGTGG TGTCCTGCCT CAAAGAAACG 
GTCTGGACCA AGCTCTCGAC ACCCCGGGAG GAGAAGCAGT TTCTGGAGCT CTTGAGCTGC 
CTGGTGAGTC CTACAAAGCC CCAAGGGATT CCAGTCGCTG CTCTGCTCGA GCCGGATGAG 
GTACTAAAGG AGTTCGTCCT GCCTTTCTTG ATGCTGGAGG TCAAAGAGGT GGACCTCAGT 
CTGAGGATCT TCGTCCAGAC TCTGGAAGGG AGCACAAACC TGGAAGAATA CTGGCTGCAG 
TCCTGCTCTC CGTTCCCGCT CATCTTCAGC CTGTGCCAGC TCCTTGATGG CTTCAGCAAA 
TACTGGCAGC TCCCCAAGGA GAAGCGCTGT CTCCCCCTGG ACGGGAAGGA CCTGGCCATC 
CACATCCTGG AGCTCCTCTG TGAGGTGGTC TCAGCTAACA CCGACACCTT CTCCCCAGAC 
ACCTGGGTCA AGTCGCTGTC CTGGCTCCAC CGGAAGCTGG AGCAGCTAGA CTGGACTGTG 
GGCCTGAGAC TGAAGAGCTT CTTTGAGGGC CACTTCAAGT GCGAGGTGCC AGCCACGCTC 
TTTGAGATCT GTAAGCTTTC TGAGGACGAG TGGACCTCCC AGGCCCACCC CGGGTATGGG 
CCTGGCACAG GCCTCCTGGC CTGGATGGAG TGCTCCTCCA TCTCCAGCAG CGTCTCCGAG 
CAGATGCTCG CACTCCTGGT GGTGGACGTG GGCAACCCTG AGGAGGTCAG GTTGTTCAGC 
AAGGGCTTCC TGGTGGCCCT GGTACAGGTC ATGCCATGGT GCAGCCCCCA GGAATGGCAG 
TGCCTTCACC AGCTGACCCG GAGACTGTTG GAGAAGCAAC TCCTGCACGT CCCCTACAGC 
CTGGAGTATA TACAGTTCGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCA GGAGCTCCAG 
CTCTCTGTCC TCTTGCTTAG AGTTTTTCAG TTTCTCTGCA GCCAGAGCTG CCGTAACTGG 
CTCCCTGTGG AGGGCTGGAG CCATGTGGTC AAGCTCCTCT GCAACAGTCT GACCAATCTC 
CTGGACTCTG TTCGGTTGAT ACAGTCGGTT GGCCCTTGGG CTCAAGGACA AGAGCAGGAC 
CTGACCCAGG AAACCCTCTT TTTCTATACC CAGGTCTTCT GTCATGTTCT GCACATCATG 
GCCATGGTCC ACCAGGAGGT CTGTGAACCT CTCTATGTCT TGGCCTTGGA GATCCTCACC 
TGCTACGAAA CCCTGAGCAA GACCAACCCT TCCGTCAGCT CCTTGCTCCA GAAAGTAAAC 
GAGCAGCGCT TCTTAAAGTC CATCGCCGAG AACATCAGCC CTGAGGAGCG GCGCCAAACC 
CTCCTGCAGA AGATCAGCAA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_010824017.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822319.1 Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa54209
Clone ID Related Accession (Same CDS sequence)	XM_010815870.2 , XM_010824017.2
Accession Version	XM_010824017.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	gem-associated protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTATCT TGCACGGGGG CTTCTTGCTG GCTGAGCAGC TGTTCCGCCC CAAAGCGCTG 
GCCGAACTGA CCAAGTCTGA CTGGGTGCAC GTTGGGCGGC CCATTGTGGA GGCTCTGAGG 
GAGATCTCCT CCACCACAGC CTGCTCCCAG CCCTTCGCCT GGAAGAAGAA AGCGCTGATC 
ATCATCTGGG CCAAGGTGCT ACAGCCCTAC CCCATCACCC CTTCCGACAT GGAAACCCGC 
TGGCAGGAAG ATGTGTTCTT CTCTGTGGGC AACATGATCC CCACCATCAA TCACACAGTC 
CTCTTTGAGC TGCTCAAGTC GCTGGAGGCC TCTGGACTCT TTATCCAGCT CCTGATGGCC 
CTGCCCACCA CTGTCTGCCG TGCGGAACTA GAGCACTTTT TGGAGCACAT GACCATCGAC 
ACCTCTTCCA AGGACGTGGC CTTCTTCCTC GACGTCTGGT GGGAAATGAT GAAGCACAAG 
GGCAATCAGC AGGATCCCCT GCTCTCCCAG TTCCGGACAA TGGCCCACAA GTACCTGTCC 
TCCTCCGATG ACTTCTCCCA CCCGCCGAAG AGGTTCAAGT CCGATCCAGA TGTGTGTCCA 
ACCATGCCCC TGCTGGCCAT GCTGCTCACG GGGCTGAAAC AAATCCAGGA CAGGATCCTA 
TGCCCCGGGA TGAAGTGCTG CGCCTTAGCC AACTTGGCTG ACATGCTGAC CGTGTTTGCG 
CTGATGGAGG AGGACCCCCA GGAAGTGTCT GCGACCGTGT ACCTGGACAA GCTGGCCACG 
GTGATCTCCG TGTGGAACTC GGACACCCAG AACCCGTATC ACCAGCAGGC GCTGGCAGAG 
AAGGTGAAGG AGGCGGAGCG GGACATCAGC CTGACCTCCC TGGCCAGGCT GCCGAGCGAG 
ACCATCTTCG TGGGCTTTGA GTTCCTGCGC AGCCTGCTGC AGGAGTGGGG GGAGGAGCTG 
CAGGCCTTGC TCAGCAGCAG CCAGGGGACC AGCTACGACA GCTTCCGGCT CTGCGACAGC 
CTGACCTCCT TCAGCCAGAA TCTGAAGCTC TACCTGGACA CCACCAGCCT GGCCAAGGAG 
GAGAGGCAGG TGGTCTCCGA GCTGGCAGAG TGCGTGGGGG ACTTCCTGAG GAAGACGAAC 
AGGGTGGTGA GGAGCAAGGG CGGTGAGCAG GACATCACCG CCTCCATCGC CATGGCCATC 
ATCGAGCAGA AGATGGACCG CCACATGGAA ATGTGCTACA TTTTTGCCTC CGAGAGGAAG 
TGGGCCTTCT CGGACGAGTG GCTGTCCTGC CTGCTCAACA ACCGAGCTCT CTTCCGAGAG 
CCGGGCCTGG TGTTAAAGCT GCTGGAGACG GTGATGGAAG TCAGCACGAT AGACAGGGCT 
GTCTCCGAAC CTCAGATCAA ACAGGTGTTC GACTTGATCC TGGAGTGTTA CACACACCTC 
TCTCTGCCAG ATAAAAATAA AGTCCTCTCG GGGGTCCTGC TCTCCTGGGG GCGCAAGGGC 
CTCTCTGAGA AATTGTTAGC TCACTTGGAG GGCTTTCAGG AGGACCTCAA CACGACTTTC 
AACCAGCTCA CACAGAGCAC CTCTGAACAG GGCTTGGCTA AGGCTGTTGC TTCCGTGGCC 
CGCCTGGTCA TCCTGCACCC AGAGATCACG GTGAAGAAGT TGTGCAGCAT GGCTGTGGTC 
AACCTCGGCA CCCACAAGTT CCTGGCTCAG ATTCTCACTG CCTTCCCCGC TCTCCGGTTC 
ATGGAAGAGC AGGGGCCAGA CCGCTCTACC ACATTCGTGG TGTCCTGCCT CAAAGAAACG 
GTCTGGACCA AGCTCTCGAC ACCCCGGGAG GAGAAGCAGT TTCTGGAGCT CTTGAGCTGC 
CTGGTGAGTC CTACAAAGCC CCAAGGGATT CCAGTCGCTG CTCTGCTCGA GCCGGATGAG 
GTACTAAAGG AGTTCGTCCT GCCTTTCTTG ATGCTGGAGG TCAAAGAGGT GGACCTCAGT 
CTGAGGATCT TCGTCCAGAC TCTGGAAGGG AGCACAAACC TGGAAGAATA CTGGCTGCAG 
TCCTGCTCTC CGTTCCCGCT CATCTTCAGC CTGTGCCAGC TCCTTGATGG CTTCAGCAAA 
TACTGGCAGC TCCCCAAGGA GAAGCGCTGT CTCCCCCTGG ACGGGAAGGA CCTGGCCATC 
CACATCCTGG AGCTCCTCTG TGAGGTGGTC TCAGCTAACA CCGACACCTT CTCCCCAGAC 
ACCTGGGTCA AGTCGCTGTC CTGGCTCCAC CGGAAGCTGG AGCAGCTAGA CTGGACTGTG 
GGCCTGAGAC TGAAGAGCTT CTTTGAGGGC CACTTCAAGT GCGAGGTGCC AGCCACGCTC 
TTTGAGATCT GTAAGCTTTC TGAGGACGAG TGGACCTCCC AGGCCCACCC CGGGTATGGG 
CCTGGCACAG GCCTCCTGGC CTGGATGGAG TGCTCCTCCA TCTCCAGCAG CGTCTCCGAG 
CAGATGCTCG CACTCCTGGT GGTGGACGTG GGCAACCCTG AGGAGGTCAG GTTGTTCAGC 
AAGGGCTTCC TGGTGGCCCT GGTACAGGTC ATGCCATGGT GCAGCCCCCA GGAATGGCAG 
TGCCTTCACC AGCTGACCCG GAGACTGTTG GAGAAGCAAC TCCTGCACGT CCCCTACAGC 
CTGGAGTATA TACAGTTCGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCA GGAGCTCCAG 
CTCTCTGTCC TCTTGCTTAG AGTTTTTCAG TTTCTCTGCA GCCAGAGCTG CCGTAACTGG 
CTCCCTGTGG AGGGCTGGAG CCATGTGGTC AAGCTCCTCT GCAACAGTCT GACCAATCTC 
CTGGACTCTG TTCGGTTGAT ACAGTCGGTT GGCCCTTGGG CTCAAGGACA AGAGCAGGAC 
CTGACCCAGG AAACCCTCTT TTTCTATACC CAGGTCTTCT GTCATGTTCT GCACATCATG 
GCCATGGTCC ACCAGGAGGT CTGTGAACCT CTCTATGTCT TGGCCTTGGA GATCCTCACC 
TGCTACGAAA CCCTGAGCAA GACCAACCCT TCCGTCAGCT CCTTGCTCCA GAAAGTAAAC 
GAGCAGCGCT TCTTAAAGTC CATCGCCGAG AACATCAGCC CTGAGGAGCG GCGCCAAACC 
CTCCTGCAGA AGATCAGCAA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822319.1
CDS	191..3337
Translation	MTILHGGFLLAEQLFRPKALAELTKSDWVHVGRPIVEALREISSTTACSQPFAWKKKALIIIWAKVLQPYPITPSDMETRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTVCRAELEHFLEHMTIDTSSKDVAFFLDVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDDFSHPPKRFKSDPDVCPTMPLLAMLLTGLKQIQDRILCPGMKCCALANLADMLTVFALMEEDPQEVSATVYLDKLATVISVWNSDTQNPYHQQALAEKVKEAERDISLTSLARLPSETIFVGFEFLRSLLQEWGEELQALLSSSQGTSYDSFRLCDSLTSFSQNLKLYLDTTSLAKEERQVVSELAECVGDFLRKTNRVVRSKGGEQDITASIAMAIIEQKMDRHMEMCYIFASERKWAFSDEWLSCLLNNRALFREPGLVLKLLETVMEVSTIDRAVSEPQIKQVFDLILECYTHLSLPDKNKVLSGVLLSWGRKGLSEKLLAHLEGFQEDLNTTFNQLTQSTSEQGLAKAVASVARLVILHPEITVKKLCSMAVVNLGTHKFLAQILTAFPALRFMEEQGPDRSTTFVVSCLKETVWTKLSTPREEKQFLELLSCLVSPTKPQGIPVAALLEPDEVLKEFVLPFLMLEVKEVDLSLRIFVQTLEGSTNLEEYWLQSCSPFPLIFSLCQLLDGFSKYWQLPKEKRCLPLDGKDLAIHILELLCEVVSANTDTFSPDTWVKSLSWLHRKLEQLDWTVGLRLKSFFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECSSISSSVSEQMLALLVVDVGNPEEVRLFSKGFLVALVQVMPWCSPQEWQCLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQLSVLLLRVFQFLCSQSCRNWLPVEGWSHVVKLLCNSLTNLLDSVRLIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMVHQEVCEPLYVLALEILTCYETLSKTNPSVSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_010824017.2
Organism	Bos taurus(cattle)
Definition	Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824017.2

ORF Insert Sequence:

ATGACTATCT TGCACGGGGG CTTCTTGCTG GCTGAGCAGC TGTTCCGCCC CAAAGCGCTG 
GCCGAACTGA CCAAGTCTGA CTGGGTGCAC GTTGGGCGGC CCATTGTGGA GGCTCTGAGG 
GAGATCTCCT CCACCACAGC CTGCTCCCAG CCCTTCGCCT GGAAGAAGAA AGCGCTGATC 
ATCATCTGGG CCAAGGTGCT ACAGCCCTAC CCCATCACCC CTTCCGACAT GGAAACCCGC 
TGGCAGGAAG ATGTGTTCTT CTCTGTGGGC AACATGATCC CCACCATCAA TCACACAGTC 
CTCTTTGAGC TGCTCAAGTC GCTGGAGGCC TCTGGACTCT TTATCCAGCT CCTGATGGCC 
CTGCCCACCA CTGTCTGCCG TGCGGAACTA GAGCACTTTT TGGAGCACAT GACCATCGAC 
ACCTCTTCCA AGGACGTGGC CTTCTTCCTC GACGTCTGGT GGGAAATGAT GAAGCACAAG 
GGCAATCAGC AGGATCCCCT GCTCTCCCAG TTCCGGACAA TGGCCCACAA GTACCTGTCC 
TCCTCCGATG ACTTCTCCCA CCCGCCGAAG AGGTTCAAGT CCGATCCAGA TGTGTGTCCA 
ACCATGCCCC TGCTGGCCAT GCTGCTCACG GGGCTGAAAC AAATCCAGGA CAGGATCCTA 
TGCCCCGGGA TGAAGTGCTG CGCCTTAGCC AACTTGGCTG ACATGCTGAC CGTGTTTGCG 
CTGATGGAGG AGGACCCCCA GGAAGTGTCT GCGACCGTGT ACCTGGACAA GCTGGCCACG 
GTGATCTCCG TGTGGAACTC GGACACCCAG AACCCGTATC ACCAGCAGGC GCTGGCAGAG 
AAGGTGAAGG AGGCGGAGCG GGACATCAGC CTGACCTCCC TGGCCAGGCT GCCGAGCGAG 
ACCATCTTCG TGGGCTTTGA GTTCCTGCGC AGCCTGCTGC AGGAGTGGGG GGAGGAGCTG 
CAGGCCTTGC TCAGCAGCAG CCAGGGGACC AGCTACGACA GCTTCCGGCT CTGCGACAGC 
CTGACCTCCT TCAGCCAGAA TCTGAAGCTC TACCTGGACA CCACCAGCCT GGCCAAGGAG 
GAGAGGCAGG TGGTCTCCGA GCTGGCAGAG TGCGTGGGGG ACTTCCTGAG GAAGACGAAC 
AGGGTGGTGA GGAGCAAGGG CGGTGAGCAG GACATCACCG CCTCCATCGC CATGGCCATC 
ATCGAGCAGA AGATGGACCG CCACATGGAA ATGTGCTACA TTTTTGCCTC CGAGAGGAAG 
TGGGCCTTCT CGGACGAGTG GCTGTCCTGC CTGCTCAACA ACCGAGCTCT CTTCCGAGAG 
CCGGGCCTGG TGTTAAAGCT GCTGGAGACG GTGATGGAAG TCAGCACGAT AGACAGGGCT 
GTCTCCGAAC CTCAGATCAA ACAGGTGTTC GACTTGATCC TGGAGTGTTA CACACACCTC 
TCTCTGCCAG ATAAAAATAA AGTCCTCTCG GGGGTCCTGC TCTCCTGGGG GCGCAAGGGC 
CTCTCTGAGA AATTGTTAGC TCACTTGGAG GGCTTTCAGG AGGACCTCAA CACGACTTTC 
AACCAGCTCA CACAGAGCAC CTCTGAACAG GGCTTGGCTA AGGCTGTTGC TTCCGTGGCC 
CGCCTGGTCA TCCTGCACCC AGAGATCACG GTGAAGAAGT TGTGCAGCAT GGCTGTGGTC 
AACCTCGGCA CCCACAAGTT CCTGGCTCAG ATTCTCACTG CCTTCCCCGC TCTCCGGTTC 
ATGGAAGAGC AGGGGCCAGA CCGCTCTACC ACATTCGTGG TGTCCTGCCT CAAAGAAACG 
GTCTGGACCA AGCTCTCGAC ACCCCGGGAG GAGAAGCAGT TTCTGGAGCT CTTGAGCTGC 
CTGGTGAGTC CTACAAAGCC CCAAGGGATT CCAGTCGCTG CTCTGCTCGA GCCGGATGAG 
GTACTAAAGG AGTTCGTCCT GCCTTTCTTG ATGCTGGAGG TCAAAGAGGT GGACCTCAGT 
CTGAGGATCT TCGTCCAGAC TCTGGAAGGG AGCACAAACC TGGAAGAATA CTGGCTGCAG 
TCCTGCTCTC CGTTCCCGCT CATCTTCAGC CTGTGCCAGC TCCTTGATGG CTTCAGCAAA 
TACTGGCAGC TCCCCAAGGA GAAGCGCTGT CTCCCCCTGG ACGGGAAGGA CCTGGCCATC 
CACATCCTGG AGCTCCTCTG TGAGGTGGTC TCAGCTAACA CCGACACCTT CTCCCCAGAC 
ACCTGGGTCA AGTCGCTGTC CTGGCTCCAC CGGAAGCTGG AGCAGCTAGA CTGGACTGTG 
GGCCTGAGAC TGAAGAGCTT CTTTGAGGGC CACTTCAAGT GCGAGGTGCC AGCCACGCTC 
TTTGAGATCT GTAAGCTTTC TGAGGACGAG TGGACCTCCC AGGCCCACCC CGGGTATGGG 
CCTGGCACAG GCCTCCTGGC CTGGATGGAG TGCTCCTCCA TCTCCAGCAG CGTCTCCGAG 
CAGATGCTCG CACTCCTGGT GGTGGACGTG GGCAACCCTG AGGAGGTCAG GTTGTTCAGC 
AAGGGCTTCC TGGTGGCCCT GGTACAGGTC ATGCCATGGT GCAGCCCCCA GGAATGGCAG 
TGCCTTCACC AGCTGACCCG GAGACTGTTG GAGAAGCAAC TCCTGCACGT CCCCTACAGC 
CTGGAGTATA TACAGTTCGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCA GGAGCTCCAG 
CTCTCTGTCC TCTTGCTTAG AGTTTTTCAG TTTCTCTGCA GCCAGAGCTG CCGTAACTGG 
CTCCCTGTGG AGGGCTGGAG CCATGTGGTC AAGCTCCTCT GCAACAGTCT GACCAATCTC 
CTGGACTCTG TTCGGTTGAT ACAGTCGGTT GGCCCTTGGG CTCAAGGACA AGAGCAGGAC 
CTGACCCAGG AAACCCTCTT TTTCTATACC CAGGTCTTCT GTCATGTTCT GCACATCATG 
GCCATGGTCC ACCAGGAGGT CTGTGAACCT CTCTATGTCT TGGCCTTGGA GATCCTCACC 
TGCTACGAAA CCCTGAGCAA GACCAACCCT TCCGTCAGCT CCTTGCTCCA GAAAGTAAAC 
GAGCAGCGCT TCTTAAAGTC CATCGCCGAG AACATCAGCC CTGAGGAGCG GCGCCAAACC 
CTCCTGCAGA AGATCAGCAA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GEMIN4
Entrez Gene ID	539147
Full Name	gem nuclear organelle associated protein 4
General protein information	Preferred Names gem nuclear organelle associated protein 4 Names gem-associated protein 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	GEMIN4	XP_001084635.1
Gallus gallus (chicken)	GEMIN4	NP_001012628.1
Xenopus tropicalis (tropical clawed frog)	gemin4	XP_004911736.1
Canis lupus familiaris (dog)	GEMIN4	XP_853922.2
Rattus norvegicus (Norway rat)	Gemin4	NP_001102507.1
Danio rerio (zebrafish)	gemin4	NP_001018154.1
Mus musculus (house mouse)	Gemin4	NP_796341.2
Homo sapiens (human)	GEMIN4	NP_056536.2
Pan troglodytes (chimpanzee)	GEMIN4	XP_511244.2
Bos taurus (cattle)	GEMIN4	XP_002695725.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GEMIN4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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