GEMIN4 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	GEMIN4
Entrez Gene ID	611189
Full Name	gem nuclear organelle associated protein 4
General protein information	Preferred Names gem nuclear organelle associated protein 4 Names gem-associated protein 4
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005624913.3	XP_005624970.1	gem-associated protein 4 isoform X2
XM_005624913.2	XP_005624970.1	gem-associated protein 4 isoform X2
XM_848829.4	XP_853922.2	gem-associated protein 4 isoform X1
XM_005624915.3	XP_005624972.1	gem-associated protein 4 isoform X2
XM_005624914.2	XP_005624971.1	gem-associated protein 4 isoform X2
XM_005624915.2	XP_005624972.1	gem-associated protein 4 isoform X2
XM_848829.5	XP_853922.2	gem-associated protein 4 isoform X1

Pathways from BioSystems

Homologies

	GEMIN4	XP_001084635.1
Gallus gallus (chicken)	GEMIN4	NP_001012628.1
Xenopus tropicalis (tropical clawed frog)	gemin4	XP_004911736.1
Canis lupus familiaris (dog)	GEMIN4	XP_853922.2
Rattus norvegicus (Norway rat)	Gemin4	NP_001102507.1
Danio rerio (zebrafish)	gemin4	NP_001018154.1
Mus musculus (house mouse)	Gemin4	NP_796341.2
Homo sapiens (human)	GEMIN4	NP_056536.2
Pan troglodytes (chimpanzee)	GEMIN4	XP_511244.2
Bos taurus (cattle)	GEMIN4	XP_002695725.1

Gene Ontology

The following GEMIN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GEMIN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe12298	XM_848829.5 Latest version!	Canis lupus familiaris gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe12299	XM_005624915.3 Latest version!	Canis lupus familiaris gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe12299	XM_005624914.2 Latest version!	Canis lupus familiaris gem (nuclear organelle) associated protein 4 (GEMIN4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe12299	XM_005624913.3 Latest version!	Canis lupus familiaris gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OCe12299	XM_005624913.2	Canis lupus familiaris gem (nuclear organelle) associated protein 4 (GEMIN4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe12299	XM_005624915.2	Canis lupus familiaris gem (nuclear organelle) associated protein 4 (GEMIN4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe12298	XM_848829.4	Canis lupus familiaris gem (nuclear organelle) associated protein 4 (GEMIN4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GEMIN4 ( XM_848829.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_853922.2 Canis lupus familiaris gem (nuclear organelle) associated protein 4 (GEMIN4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe12298
Clone ID Related Accession (Same CDS sequence)	XM_848829.4 , XM_848829.5
Accession Version	XM_848829.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	gem-associated protein 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_848829.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTAG GACCTTTGAA TATCTGTGAA GAAATGACTA TTCTGCATGG GGGCTTTTTG 
CTTGCCGAGC AGCTCTTCCA CCCCAAAGCA CTGACAGAAC TGACAAAGTC TGACTGGGAA 
CATGTTGGGC AGCCCATTGT GGAGGCCCTA AGGGAGATCT CCTCCGCCAC AACATGCTCC 
CAGCCCTTTG CCTGGAAGAA GAAAGCTCTG ATCATCATCT GGGCCAAGGT TCTTCAGCCC 
TACCCTGTCA CCCCTTCAGA CACTGACACT CGGTGGCAGG AAGATGTGTT CTTCTCGGTA 
GGCAACATGA TCCCTACCAT CAATCACACG GTCCTTTTTG AGCTGCTCAA GTCTCTGGAA 
GCTTCTGGAC TCTTTATCCA GCTTCTGATG GCCCTGCCCA CCACCATCTG CCATGCAGAA 
CTAGAGCGCT TTTTGGAGCA CATGTCTGTT GACACTTCTT CAGAGGATGT GGCCTTTTTC 
CTCAGTGTCT GGTGGGAAAT GATGAAGCAC AAAGGCAATC AGCAGGATCC TCTGCTCTCC 
CAGTTTAGAA CAATGGCCCA TAAGTACTTG TCTTCTTCAG ATGAGTTCTC CCATCCTCCA 
AAGAGGTTTA AGTCAGACCC AGAGGTGTGT CCCACTATGC CCCTGTTGGC CATGCTGCTC 
ACTGGGCTAA AGCAAATCCA AAATAGAATC CTGTGCCCAG GGATGAAATG CTGCGCATTA 
GCCAACTTAG CAGACATGCT AACAGTATTT GCACTGATGG AGGATGACCC CCAGGAAGTG 
TCTGCAACTG TGTATCTGGA CAAACTGGCC ACAGTGATCT CCGTGTGGAA TTCGGACACC 
CAGAACCCGT ATTACCAACA GGCACTAGTA GAGAAGGTAA AGGAGGCAGA ACGGGACGTC 
AGCCTGACCT CCCTGGCCAA GCTTCCAAGC GAGACGATTT TCACTGGGTT TGAGTTCATG 
TGCAGCCTCC TGCGGGAGTG GGGGAAGGAG CTGCAGACCA TGCTCAACAG CAGCCAGGGG 
ACAAGTTATG ACAGCTACCG GCTGTGTGAC AGCCTGACTT CCTTCAGCCA GAACTTGAAG 
CTCTACCTTG ATTCTACCAG CTTGTCTAAG GAGGAGCGGC AGGTGGTCTC AGAGCTAGCA 
GAGTGTGTCG AAGACTTCCT GAGGAAAACA AACAGGGTGC TGAAGAATAA GGGCTTTGAG 
AAGGACATCA CCGCCTCGAT TGCCATGGCC ATCATTGAGC AGAAGATGGA CCGGCATATG 
GAAATGTGCT ACATTTTTGC TTCTGAGAAG AAGTGGGCCT TCTCAGACCA GTGGCTAGCC 
TGCCTGGTTA ATAACTGGGC TCTCTTCCGA GAGCCAGACT TGGTGTTAAA GCTGCTGGAA 
ACAGTGATGG AAGTTACTGC GACAGACAGA GCCATCCCTG AATCTCAGAT CAAACAAGTG 
ATTGGCTTGA TCCTGGAATG CTACGCAGAC CTCTCGCTGC CAGATAAAAA TAAAGTCCTC 
TCAGGCACCC TGCTTTCCTG GGGGCGGAAG GGTCTCTCTG AGAACCTGTT GGCTTACTTG 
GACGGGTTTC AGGAGGACCT CAACACAACT TTTAACCAGC TTGCGCAGAG TGCCTCTGAA 
CAGGGCTTAG CTAAAGCGGT GGCTTCTGTG GCCCGCCTGA TAATATTGCA CCCGGAGATC 
ACAGTGAAGA AAATGTGCAG CATGGCTGTG GTCAACCTTG GCACCCACAA ATTCCTGGCT 
CAGATTCTCT CTGCCTTCCC TGCCCTTAGG TTCACCGAAG AGCAGGATCC AAATGCATCT 
ACTACCTTTG TGGTGTCATG CCTCAAAGAA ACTGTTTGGA CAAAGTTCTC TACACCTAAG 
GAAGAAAAGC AATTTTTGGA GCTCCTGGGC TGCCTGGTGA GTCCTGTGAA GCCCCAGGGT 
ATCCCGGTTG CTGCTCTTCT TGAGCCACAT GAGGTGCTAA AGGAGTTTGT CCTGCCTTTC 
TTGATGCTTG ATGTCAAAGA GGTAGACCTC AGTTTGAAGA TTTTCCTCCA GACTCTTGAA 
GCAAATGCAT GTTTAGAGGA ATACTGGCTC CAGACCTGTT CTCCATTCCC CCTCATCTTC 
AGTTTGTGCC AGCTCCTGGA CTGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAACGG 
TGCCTCTTGT TGGATGGGAA GGATCTGGTG GTCCATATCC TGGAGATCCT CTGTGAGATC 
GTACTGGCTA ATGCTGAGAC ATTTTCCCCA GACACCTGGA TCAAGTCCCT GTCTTGGCTC 
CACCGGAAGT TGGAGCAGCT CGACTGGACA GTGCGCCTGA GGATGAAGAA CCTCTTTGAG 
GGCCACTTCA AATGTGAGGT GCCAGCCACG CTTTTTGAGA TCTGCAAGCT TTCTGAGGAT 
GAATGGACTT CCCAGGCCCA CCCTGGGTAT GGGCCAGGCA CAGGGCTTCT TGCCTGGATG 
GAGTGCTGCT ACATTTCCAG CAGCATCTCT GAGCAGATGC TGTCCCTCTT GGTGGTAGAT 
GTGGGTAACC CTGAAGAGGT CAGATTGTTC AGCAAGGGCT TTCTGGTGGC CCTGGTACAA 
GTCATGCCTT GGTGCAGCTC CCAAGAGTGG CAGTACCTTT ACCAGCTGAC CAGGAGACTA 
TTGGAGAAAC AACTCCTACA TGTCCCTTAT AGCCTGGAAT ATATTCAATT TGTTCCTCTG 
CTCAACCTGA AGCCCTTTGC CCCGGAGCTC CAGCTGTCCG TACTCTTGCT GAGAGTTTTC 
CAGTTTCTCT GCAGCCAGAG TTGTCGTAAC TGGCTTCCTA TAGAAGGCTG GAGCCATGTA 
GTCAGGCTTC TCTGTAACAG TCTGACCAAT CTCCTGGACT CCGTTAGGAT GATACAGTCA 
GTTGGCCCTT GGGCTCAAGG ACAAGAACAG GACCTGACCC AGGAAACCCT GTTTTTTTAT 
ACCCAGGTAT TCTGTCACGT TCTGCACATC ATGGCCATGC TCCCCCACGA GGTGTGCGAG 
CCGCTGTATG TCTTGGCCTT GGAAATCCTC ACCTGCTATG AAACCCTAAG CAAGGCTAAT 
CCTTCTAGTA GTTCCCTGCT CCAGAAGGTA AATGAGCAGC GCTTCTTAAA GTCCATCGCT 
GAGAACATTA GCCCTGAGGA ACGGCGCCAA ACCCTGCTGC AGAAGATCAG CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_853922.2
CDS	64..3243
Translation	MDLGPLNICEEMTILHGGFLLAEQLFHPKALTELTKSDWEHVGQPIVEALREISSATTCSQPFAWKKKALIIIWAKVLQPYPVTPSDTDTRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTICHAELERFLEHMSVDTSSEDVAFFLSVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDEFSHPPKRFKSDPEVCPTMPLLAMLLTGLKQIQNRILCPGMKCCALANLADMLTVFALMEDDPQEVSATVYLDKLATVISVWNSDTQNPYYQQALVEKVKEAERDVSLTSLAKLPSETIFTGFEFMCSLLREWGKELQTMLNSSQGTSYDSYRLCDSLTSFSQNLKLYLDSTSLSKEERQVVSELAECVEDFLRKTNRVLKNKGFEKDITASIAMAIIEQKMDRHMEMCYIFASEKKWAFSDQWLACLVNNWALFREPDLVLKLLETVMEVTATDRAIPESQIKQVIGLILECYADLSLPDKNKVLSGTLLSWGRKGLSENLLAYLDGFQEDLNTTFNQLAQSASEQGLAKAVASVARLIILHPEITVKKMCSMAVVNLGTHKFLAQILSAFPALRFTEEQDPNASTTFVVSCLKETVWTKFSTPKEEKQFLELLGCLVSPVKPQGIPVAALLEPHEVLKEFVLPFLMLDVKEVDLSLKIFLQTLEANACLEEYWLQTCSPFPLIFSLCQLLDCFSKYWQLPKEKRCLLLDGKDLVVHILEILCEIVLANAETFSPDTWIKSLSWLHRKLEQLDWTVRLRMKNLFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECCYISSSISEQMLSLLVVDVGNPEEVRLFSKGFLVALVQVMPWCSSQEWQYLYQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAPELQLSVLLLRVFQFLCSQSCRNWLPIEGWSHVVRLLCNSLTNLLDSVRMIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMLPHEVCEPLYVLALEILTCYETLSKANPSSSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_848829.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris gem (nuclear organelle) associated protein 4 (GEMIN4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_848829.4

ORF Insert Sequence:

ATGGACCTAG GACCTTTGAA TATCTGTGAA GAAATGACTA TTCTGCATGG GGGCTTTTTG 
CTTGCCGAGC AGCTCTTCCA CCCCAAAGCA CTGACAGAAC TGACAAAGTC TGACTGGGAA 
CATGTTGGGC AGCCCATTGT GGAGGCCCTA AGGGAGATCT CCTCCGCCAC AACATGCTCC 
CAGCCCTTTG CCTGGAAGAA GAAAGCTCTG ATCATCATCT GGGCCAAGGT TCTTCAGCCC 
TACCCTGTCA CCCCTTCAGA CACTGACACT CGGTGGCAGG AAGATGTGTT CTTCTCGGTA 
GGCAACATGA TCCCTACCAT CAATCACACG GTCCTTTTTG AGCTGCTCAA GTCTCTGGAA 
GCTTCTGGAC TCTTTATCCA GCTTCTGATG GCCCTGCCCA CCACCATCTG CCATGCAGAA 
CTAGAGCGCT TTTTGGAGCA CATGTCTGTT GACACTTCTT CAGAGGATGT GGCCTTTTTC 
CTCAGTGTCT GGTGGGAAAT GATGAAGCAC AAAGGCAATC AGCAGGATCC TCTGCTCTCC 
CAGTTTAGAA CAATGGCCCA TAAGTACTTG TCTTCTTCAG ATGAGTTCTC CCATCCTCCA 
AAGAGGTTTA AGTCAGACCC AGAGGTGTGT CCCACTATGC CCCTGTTGGC CATGCTGCTC 
ACTGGGCTAA AGCAAATCCA AAATAGAATC CTGTGCCCAG GGATGAAATG CTGCGCATTA 
GCCAACTTAG CAGACATGCT AACAGTATTT GCACTGATGG AGGATGACCC CCAGGAAGTG 
TCTGCAACTG TGTATCTGGA CAAACTGGCC ACAGTGATCT CCGTGTGGAA TTCGGACACC 
CAGAACCCGT ATTACCAACA GGCACTAGTA GAGAAGGTAA AGGAGGCAGA ACGGGACGTC 
AGCCTGACCT CCCTGGCCAA GCTTCCAAGC GAGACGATTT TCACTGGGTT TGAGTTCATG 
TGCAGCCTCC TGCGGGAGTG GGGGAAGGAG CTGCAGACCA TGCTCAACAG CAGCCAGGGG 
ACAAGTTATG ACAGCTACCG GCTGTGTGAC AGCCTGACTT CCTTCAGCCA GAACTTGAAG 
CTCTACCTTG ATTCTACCAG CTTGTCTAAG GAGGAGCGGC AGGTGGTCTC AGAGCTAGCA 
GAGTGTGTCG AAGACTTCCT GAGGAAAACA AACAGGGTGC TGAAGAATAA GGGCTTTGAG 
AAGGACATCA CCGCCTCGAT TGCCATGGCC ATCATTGAGC AGAAGATGGA CCGGCATATG 
GAAATGTGCT ACATTTTTGC TTCTGAGAAG AAGTGGGCCT TCTCAGACCA GTGGCTAGCC 
TGCCTGGTTA ATAACTGGGC TCTCTTCCGA GAGCCAGACT TGGTGTTAAA GCTGCTGGAA 
ACAGTGATGG AAGTTACTGC GACAGACAGA GCCATCCCTG AATCTCAGAT CAAACAAGTG 
ATTGGCTTGA TCCTGGAATG CTACGCAGAC CTCTCGCTGC CAGATAAAAA TAAAGTCCTC 
TCAGGCACCC TGCTTTCCTG GGGGCGGAAG GGTCTCTCTG AGAACCTGTT GGCTTACTTG 
GACGGGTTTC AGGAGGACCT CAACACAACT TTTAACCAGC TTGCGCAGAG TGCCTCTGAA 
CAGGGCTTAG CTAAAGCGGT GGCTTCTGTG GCCCGCCTGA TAATATTGCA CCCGGAGATC 
ACAGTGAAGA AAATGTGCAG CATGGCTGTG GTCAACCTTG GCACCCACAA ATTCCTGGCT 
CAGATTCTCT CTGCCTTCCC TGCCCTTAGG TTCACCGAAG AGCAGGATCC AAATGCATCT 
ACTACCTTTG TGGTGTCATG CCTCAAAGAA ACTGTTTGGA CAAAGTTCTC TACACCTAAG 
GAAGAAAAGC AATTTTTGGA GCTCCTGGGC TGCCTGGTGA GTCCTGTGAA GCCCCAGGGT 
ATCCCGGTTG CTGCTCTTCT TGAGCCACAT GAGGTGCTAA AGGAGTTTGT CCTGCCTTTC 
TTGATGCTTG ATGTCAAAGA GGTAGACCTC AGTTTGAAGA TTTTCCTCCA GACTCTTGAA 
GCAAATGCAT GTTTAGAGGA ATACTGGCTC CAGACCTGTT CTCCATTCCC CCTCATCTTC 
AGTTTGTGCC AGCTCCTGGA CTGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAACGG 
TGCCTCTTGT TGGATGGGAA GGATCTGGTG GTCCATATCC TGGAGATCCT CTGTGAGATC 
GTACTGGCTA ATGCTGAGAC ATTTTCCCCA GACACCTGGA TCAAGTCCCT GTCTTGGCTC 
CACCGGAAGT TGGAGCAGCT CGACTGGACA GTGCGCCTGA GGATGAAGAA CCTCTTTGAG 
GGCCACTTCA AATGTGAGGT GCCAGCCACG CTTTTTGAGA TCTGCAAGCT TTCTGAGGAT 
GAATGGACTT CCCAGGCCCA CCCTGGGTAT GGGCCAGGCA CAGGGCTTCT TGCCTGGATG 
GAGTGCTGCT ACATTTCCAG CAGCATCTCT GAGCAGATGC TGTCCCTCTT GGTGGTAGAT 
GTGGGTAACC CTGAAGAGGT CAGATTGTTC AGCAAGGGCT TTCTGGTGGC CCTGGTACAA 
GTCATGCCTT GGTGCAGCTC CCAAGAGTGG CAGTACCTTT ACCAGCTGAC CAGGAGACTA 
TTGGAGAAAC AACTCCTACA TGTCCCTTAT AGCCTGGAAT ATATTCAATT TGTTCCTCTG 
CTCAACCTGA AGCCCTTTGC CCCGGAGCTC CAGCTGTCCG TACTCTTGCT GAGAGTTTTC 
CAGTTTCTCT GCAGCCAGAG TTGTCGTAAC TGGCTTCCTA TAGAAGGCTG GAGCCATGTA 
GTCAGGCTTC TCTGTAACAG TCTGACCAAT CTCCTGGACT CCGTTAGGAT GATACAGTCA 
GTTGGCCCTT GGGCTCAAGG ACAAGAACAG GACCTGACCC AGGAAACCCT GTTTTTTTAT 
ACCCAGGTAT TCTGTCACGT TCTGCACATC ATGGCCATGC TCCCCCACGA GGTGTGCGAG 
CCGCTGTATG TCTTGGCCTT GGAAATCCTC ACCTGCTATG AAACCCTAAG CAAGGCTAAT 
CCTTCTAGTA GTTCCCTGCT CCAGAAGGTA AATGAGCAGC GCTTCTTAAA GTCCATCGCT 
GAGAACATTA GCCCTGAGGA ACGGCGCCAA ACCCTGCTGC AGAAGATCAG CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_848829.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_853922.2 Canis lupus familiaris gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe12298
Clone ID Related Accession (Same CDS sequence)	XM_848829.4 , XM_848829.5
Accession Version	XM_848829.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	gem-associated protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_848829.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTAG GACCTTTGAA TATCTGTGAA GAAATGACTA TTCTGCATGG GGGCTTTTTG 
CTTGCCGAGC AGCTCTTCCA CCCCAAAGCA CTGACAGAAC TGACAAAGTC TGACTGGGAA 
CATGTTGGGC AGCCCATTGT GGAGGCCCTA AGGGAGATCT CCTCCGCCAC AACATGCTCC 
CAGCCCTTTG CCTGGAAGAA GAAAGCTCTG ATCATCATCT GGGCCAAGGT TCTTCAGCCC 
TACCCTGTCA CCCCTTCAGA CACTGACACT CGGTGGCAGG AAGATGTGTT CTTCTCGGTA 
GGCAACATGA TCCCTACCAT CAATCACACG GTCCTTTTTG AGCTGCTCAA GTCTCTGGAA 
GCTTCTGGAC TCTTTATCCA GCTTCTGATG GCCCTGCCCA CCACCATCTG CCATGCAGAA 
CTAGAGCGCT TTTTGGAGCA CATGTCTGTT GACACTTCTT CAGAGGATGT GGCCTTTTTC 
CTCAGTGTCT GGTGGGAAAT GATGAAGCAC AAAGGCAATC AGCAGGATCC TCTGCTCTCC 
CAGTTTAGAA CAATGGCCCA TAAGTACTTG TCTTCTTCAG ATGAGTTCTC CCATCCTCCA 
AAGAGGTTTA AGTCAGACCC AGAGGTGTGT CCCACTATGC CCCTGTTGGC CATGCTGCTC 
ACTGGGCTAA AGCAAATCCA AAATAGAATC CTGTGCCCAG GGATGAAATG CTGCGCATTA 
GCCAACTTAG CAGACATGCT AACAGTATTT GCACTGATGG AGGATGACCC CCAGGAAGTG 
TCTGCAACTG TGTATCTGGA CAAACTGGCC ACAGTGATCT CCGTGTGGAA TTCGGACACC 
CAGAACCCGT ATTACCAACA GGCACTAGTA GAGAAGGTAA AGGAGGCAGA ACGGGACGTC 
AGCCTGACCT CCCTGGCCAA GCTTCCAAGC GAGACGATTT TCACTGGGTT TGAGTTCATG 
TGCAGCCTCC TGCGGGAGTG GGGGAAGGAG CTGCAGACCA TGCTCAACAG CAGCCAGGGG 
ACAAGTTATG ACAGCTACCG GCTGTGTGAC AGCCTGACTT CCTTCAGCCA GAACTTGAAG 
CTCTACCTTG ATTCTACCAG CTTGTCTAAG GAGGAGCGGC AGGTGGTCTC AGAGCTAGCA 
GAGTGTGTCG AAGACTTCCT GAGGAAAACA AACAGGGTGC TGAAGAATAA GGGCTTTGAG 
AAGGACATCA CCGCCTCGAT TGCCATGGCC ATCATTGAGC AGAAGATGGA CCGGCATATG 
GAAATGTGCT ACATTTTTGC TTCTGAGAAG AAGTGGGCCT TCTCAGACCA GTGGCTAGCC 
TGCCTGGTTA ATAACTGGGC TCTCTTCCGA GAGCCAGACT TGGTGTTAAA GCTGCTGGAA 
ACAGTGATGG AAGTTACTGC GACAGACAGA GCCATCCCTG AATCTCAGAT CAAACAAGTG 
ATTGGCTTGA TCCTGGAATG CTACGCAGAC CTCTCGCTGC CAGATAAAAA TAAAGTCCTC 
TCAGGCACCC TGCTTTCCTG GGGGCGGAAG GGTCTCTCTG AGAACCTGTT GGCTTACTTG 
GACGGGTTTC AGGAGGACCT CAACACAACT TTTAACCAGC TTGCGCAGAG TGCCTCTGAA 
CAGGGCTTAG CTAAAGCGGT GGCTTCTGTG GCCCGCCTGA TAATATTGCA CCCGGAGATC 
ACAGTGAAGA AAATGTGCAG CATGGCTGTG GTCAACCTTG GCACCCACAA ATTCCTGGCT 
CAGATTCTCT CTGCCTTCCC TGCCCTTAGG TTCACCGAAG AGCAGGATCC AAATGCATCT 
ACTACCTTTG TGGTGTCATG CCTCAAAGAA ACTGTTTGGA CAAAGTTCTC TACACCTAAG 
GAAGAAAAGC AATTTTTGGA GCTCCTGGGC TGCCTGGTGA GTCCTGTGAA GCCCCAGGGT 
ATCCCGGTTG CTGCTCTTCT TGAGCCACAT GAGGTGCTAA AGGAGTTTGT CCTGCCTTTC 
TTGATGCTTG ATGTCAAAGA GGTAGACCTC AGTTTGAAGA TTTTCCTCCA GACTCTTGAA 
GCAAATGCAT GTTTAGAGGA ATACTGGCTC CAGACCTGTT CTCCATTCCC CCTCATCTTC 
AGTTTGTGCC AGCTCCTGGA CTGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAACGG 
TGCCTCTTGT TGGATGGGAA GGATCTGGTG GTCCATATCC TGGAGATCCT CTGTGAGATC 
GTACTGGCTA ATGCTGAGAC ATTTTCCCCA GACACCTGGA TCAAGTCCCT GTCTTGGCTC 
CACCGGAAGT TGGAGCAGCT CGACTGGACA GTGCGCCTGA GGATGAAGAA CCTCTTTGAG 
GGCCACTTCA AATGTGAGGT GCCAGCCACG CTTTTTGAGA TCTGCAAGCT TTCTGAGGAT 
GAATGGACTT CCCAGGCCCA CCCTGGGTAT GGGCCAGGCA CAGGGCTTCT TGCCTGGATG 
GAGTGCTGCT ACATTTCCAG CAGCATCTCT GAGCAGATGC TGTCCCTCTT GGTGGTAGAT 
GTGGGTAACC CTGAAGAGGT CAGATTGTTC AGCAAGGGCT TTCTGGTGGC CCTGGTACAA 
GTCATGCCTT GGTGCAGCTC CCAAGAGTGG CAGTACCTTT ACCAGCTGAC CAGGAGACTA 
TTGGAGAAAC AACTCCTACA TGTCCCTTAT AGCCTGGAAT ATATTCAATT TGTTCCTCTG 
CTCAACCTGA AGCCCTTTGC CCCGGAGCTC CAGCTGTCCG TACTCTTGCT GAGAGTTTTC 
CAGTTTCTCT GCAGCCAGAG TTGTCGTAAC TGGCTTCCTA TAGAAGGCTG GAGCCATGTA 
GTCAGGCTTC TCTGTAACAG TCTGACCAAT CTCCTGGACT CCGTTAGGAT GATACAGTCA 
GTTGGCCCTT GGGCTCAAGG ACAAGAACAG GACCTGACCC AGGAAACCCT GTTTTTTTAT 
ACCCAGGTAT TCTGTCACGT TCTGCACATC ATGGCCATGC TCCCCCACGA GGTGTGCGAG 
CCGCTGTATG TCTTGGCCTT GGAAATCCTC ACCTGCTATG AAACCCTAAG CAAGGCTAAT 
CCTTCTAGTA GTTCCCTGCT CCAGAAGGTA AATGAGCAGC GCTTCTTAAA GTCCATCGCT 
GAGAACATTA GCCCTGAGGA ACGGCGCCAA ACCCTGCTGC AGAAGATCAG CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_853922.2
CDS	121..3300
Translation	MDLGPLNICEEMTILHGGFLLAEQLFHPKALTELTKSDWEHVGQPIVEALREISSATTCSQPFAWKKKALIIIWAKVLQPYPVTPSDTDTRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTICHAELERFLEHMSVDTSSEDVAFFLSVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDEFSHPPKRFKSDPEVCPTMPLLAMLLTGLKQIQNRILCPGMKCCALANLADMLTVFALMEDDPQEVSATVYLDKLATVISVWNSDTQNPYYQQALVEKVKEAERDVSLTSLAKLPSETIFTGFEFMCSLLREWGKELQTMLNSSQGTSYDSYRLCDSLTSFSQNLKLYLDSTSLSKEERQVVSELAECVEDFLRKTNRVLKNKGFEKDITASIAMAIIEQKMDRHMEMCYIFASEKKWAFSDQWLACLVNNWALFREPDLVLKLLETVMEVTATDRAIPESQIKQVIGLILECYADLSLPDKNKVLSGTLLSWGRKGLSENLLAYLDGFQEDLNTTFNQLAQSASEQGLAKAVASVARLIILHPEITVKKMCSMAVVNLGTHKFLAQILSAFPALRFTEEQDPNASTTFVVSCLKETVWTKFSTPKEEKQFLELLGCLVSPVKPQGIPVAALLEPHEVLKEFVLPFLMLDVKEVDLSLKIFLQTLEANACLEEYWLQTCSPFPLIFSLCQLLDCFSKYWQLPKEKRCLLLDGKDLVVHILEILCEIVLANAETFSPDTWIKSLSWLHRKLEQLDWTVRLRMKNLFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECCYISSSISEQMLSLLVVDVGNPEEVRLFSKGFLVALVQVMPWCSSQEWQYLYQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAPELQLSVLLLRVFQFLCSQSCRNWLPIEGWSHVVRLLCNSLTNLLDSVRMIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMLPHEVCEPLYVLALEILTCYETLSKANPSSSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_848829.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_848829.5

ORF Insert Sequence:

ATGGACCTAG GACCTTTGAA TATCTGTGAA GAAATGACTA TTCTGCATGG GGGCTTTTTG 
CTTGCCGAGC AGCTCTTCCA CCCCAAAGCA CTGACAGAAC TGACAAAGTC TGACTGGGAA 
CATGTTGGGC AGCCCATTGT GGAGGCCCTA AGGGAGATCT CCTCCGCCAC AACATGCTCC 
CAGCCCTTTG CCTGGAAGAA GAAAGCTCTG ATCATCATCT GGGCCAAGGT TCTTCAGCCC 
TACCCTGTCA CCCCTTCAGA CACTGACACT CGGTGGCAGG AAGATGTGTT CTTCTCGGTA 
GGCAACATGA TCCCTACCAT CAATCACACG GTCCTTTTTG AGCTGCTCAA GTCTCTGGAA 
GCTTCTGGAC TCTTTATCCA GCTTCTGATG GCCCTGCCCA CCACCATCTG CCATGCAGAA 
CTAGAGCGCT TTTTGGAGCA CATGTCTGTT GACACTTCTT CAGAGGATGT GGCCTTTTTC 
CTCAGTGTCT GGTGGGAAAT GATGAAGCAC AAAGGCAATC AGCAGGATCC TCTGCTCTCC 
CAGTTTAGAA CAATGGCCCA TAAGTACTTG TCTTCTTCAG ATGAGTTCTC CCATCCTCCA 
AAGAGGTTTA AGTCAGACCC AGAGGTGTGT CCCACTATGC CCCTGTTGGC CATGCTGCTC 
ACTGGGCTAA AGCAAATCCA AAATAGAATC CTGTGCCCAG GGATGAAATG CTGCGCATTA 
GCCAACTTAG CAGACATGCT AACAGTATTT GCACTGATGG AGGATGACCC CCAGGAAGTG 
TCTGCAACTG TGTATCTGGA CAAACTGGCC ACAGTGATCT CCGTGTGGAA TTCGGACACC 
CAGAACCCGT ATTACCAACA GGCACTAGTA GAGAAGGTAA AGGAGGCAGA ACGGGACGTC 
AGCCTGACCT CCCTGGCCAA GCTTCCAAGC GAGACGATTT TCACTGGGTT TGAGTTCATG 
TGCAGCCTCC TGCGGGAGTG GGGGAAGGAG CTGCAGACCA TGCTCAACAG CAGCCAGGGG 
ACAAGTTATG ACAGCTACCG GCTGTGTGAC AGCCTGACTT CCTTCAGCCA GAACTTGAAG 
CTCTACCTTG ATTCTACCAG CTTGTCTAAG GAGGAGCGGC AGGTGGTCTC AGAGCTAGCA 
GAGTGTGTCG AAGACTTCCT GAGGAAAACA AACAGGGTGC TGAAGAATAA GGGCTTTGAG 
AAGGACATCA CCGCCTCGAT TGCCATGGCC ATCATTGAGC AGAAGATGGA CCGGCATATG 
GAAATGTGCT ACATTTTTGC TTCTGAGAAG AAGTGGGCCT TCTCAGACCA GTGGCTAGCC 
TGCCTGGTTA ATAACTGGGC TCTCTTCCGA GAGCCAGACT TGGTGTTAAA GCTGCTGGAA 
ACAGTGATGG AAGTTACTGC GACAGACAGA GCCATCCCTG AATCTCAGAT CAAACAAGTG 
ATTGGCTTGA TCCTGGAATG CTACGCAGAC CTCTCGCTGC CAGATAAAAA TAAAGTCCTC 
TCAGGCACCC TGCTTTCCTG GGGGCGGAAG GGTCTCTCTG AGAACCTGTT GGCTTACTTG 
GACGGGTTTC AGGAGGACCT CAACACAACT TTTAACCAGC TTGCGCAGAG TGCCTCTGAA 
CAGGGCTTAG CTAAAGCGGT GGCTTCTGTG GCCCGCCTGA TAATATTGCA CCCGGAGATC 
ACAGTGAAGA AAATGTGCAG CATGGCTGTG GTCAACCTTG GCACCCACAA ATTCCTGGCT 
CAGATTCTCT CTGCCTTCCC TGCCCTTAGG TTCACCGAAG AGCAGGATCC AAATGCATCT 
ACTACCTTTG TGGTGTCATG CCTCAAAGAA ACTGTTTGGA CAAAGTTCTC TACACCTAAG 
GAAGAAAAGC AATTTTTGGA GCTCCTGGGC TGCCTGGTGA GTCCTGTGAA GCCCCAGGGT 
ATCCCGGTTG CTGCTCTTCT TGAGCCACAT GAGGTGCTAA AGGAGTTTGT CCTGCCTTTC 
TTGATGCTTG ATGTCAAAGA GGTAGACCTC AGTTTGAAGA TTTTCCTCCA GACTCTTGAA 
GCAAATGCAT GTTTAGAGGA ATACTGGCTC CAGACCTGTT CTCCATTCCC CCTCATCTTC 
AGTTTGTGCC AGCTCCTGGA CTGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAACGG 
TGCCTCTTGT TGGATGGGAA GGATCTGGTG GTCCATATCC TGGAGATCCT CTGTGAGATC 
GTACTGGCTA ATGCTGAGAC ATTTTCCCCA GACACCTGGA TCAAGTCCCT GTCTTGGCTC 
CACCGGAAGT TGGAGCAGCT CGACTGGACA GTGCGCCTGA GGATGAAGAA CCTCTTTGAG 
GGCCACTTCA AATGTGAGGT GCCAGCCACG CTTTTTGAGA TCTGCAAGCT TTCTGAGGAT 
GAATGGACTT CCCAGGCCCA CCCTGGGTAT GGGCCAGGCA CAGGGCTTCT TGCCTGGATG 
GAGTGCTGCT ACATTTCCAG CAGCATCTCT GAGCAGATGC TGTCCCTCTT GGTGGTAGAT 
GTGGGTAACC CTGAAGAGGT CAGATTGTTC AGCAAGGGCT TTCTGGTGGC CCTGGTACAA 
GTCATGCCTT GGTGCAGCTC CCAAGAGTGG CAGTACCTTT ACCAGCTGAC CAGGAGACTA 
TTGGAGAAAC AACTCCTACA TGTCCCTTAT AGCCTGGAAT ATATTCAATT TGTTCCTCTG 
CTCAACCTGA AGCCCTTTGC CCCGGAGCTC CAGCTGTCCG TACTCTTGCT GAGAGTTTTC 
CAGTTTCTCT GCAGCCAGAG TTGTCGTAAC TGGCTTCCTA TAGAAGGCTG GAGCCATGTA 
GTCAGGCTTC TCTGTAACAG TCTGACCAAT CTCCTGGACT CCGTTAGGAT GATACAGTCA 
GTTGGCCCTT GGGCTCAAGG ACAAGAACAG GACCTGACCC AGGAAACCCT GTTTTTTTAT 
ACCCAGGTAT TCTGTCACGT TCTGCACATC ATGGCCATGC TCCCCCACGA GGTGTGCGAG 
CCGCTGTATG TCTTGGCCTT GGAAATCCTC ACCTGCTATG AAACCCTAAG CAAGGCTAAT 
CCTTCTAGTA GTTCCCTGCT CCAGAAGGTA AATGAGCAGC GCTTCTTAAA GTCCATCGCT 
GAGAACATTA GCCCTGAGGA ACGGCGCCAA ACCCTGCTGC AGAAGATCAG CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_005624913.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624970.1 Canis lupus familiaris gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe12299
Clone ID Related Accession (Same CDS sequence)	XM_005624913.3 , XM_005624913.2 , XM_005624915.3 , XM_005624914.2 , XM_005624915.2
Accession Version	XM_005624913.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	gem-associated protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624913.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTATTC TGCATGGGGG CTTTTTGCTT GCCGAGCAGC TCTTCCACCC CAAAGCACTG 
ACAGAACTGA CAAAGTCTGA CTGGGAACAT GTTGGGCAGC CCATTGTGGA GGCCCTAAGG 
GAGATCTCCT CCGCCACAAC ATGCTCCCAG CCCTTTGCCT GGAAGAAGAA AGCTCTGATC 
ATCATCTGGG CCAAGGTTCT TCAGCCCTAC CCTGTCACCC CTTCAGACAC TGACACTCGG 
TGGCAGGAAG ATGTGTTCTT CTCGGTAGGC AACATGATCC CTACCATCAA TCACACGGTC 
CTTTTTGAGC TGCTCAAGTC TCTGGAAGCT TCTGGACTCT TTATCCAGCT TCTGATGGCC 
CTGCCCACCA CCATCTGCCA TGCAGAACTA GAGCGCTTTT TGGAGCACAT GTCTGTTGAC 
ACTTCTTCAG AGGATGTGGC CTTTTTCCTC AGTGTCTGGT GGGAAATGAT GAAGCACAAA 
GGCAATCAGC AGGATCCTCT GCTCTCCCAG TTTAGAACAA TGGCCCATAA GTACTTGTCT 
TCTTCAGATG AGTTCTCCCA TCCTCCAAAG AGGTTTAAGT CAGACCCAGA GGTGTGTCCC 
ACTATGCCCC TGTTGGCCAT GCTGCTCACT GGGCTAAAGC AAATCCAAAA TAGAATCCTG 
TGCCCAGGGA TGAAATGCTG CGCATTAGCC AACTTAGCAG ACATGCTAAC AGTATTTGCA 
CTGATGGAGG ATGACCCCCA GGAAGTGTCT GCAACTGTGT ATCTGGACAA ACTGGCCACA 
GTGATCTCCG TGTGGAATTC GGACACCCAG AACCCGTATT ACCAACAGGC ACTAGTAGAG 
AAGGTAAAGG AGGCAGAACG GGACGTCAGC CTGACCTCCC TGGCCAAGCT TCCAAGCGAG 
ACGATTTTCA CTGGGTTTGA GTTCATGTGC AGCCTCCTGC GGGAGTGGGG GAAGGAGCTG 
CAGACCATGC TCAACAGCAG CCAGGGGACA AGTTATGACA GCTACCGGCT GTGTGACAGC 
CTGACTTCCT TCAGCCAGAA CTTGAAGCTC TACCTTGATT CTACCAGCTT GTCTAAGGAG 
GAGCGGCAGG TGGTCTCAGA GCTAGCAGAG TGTGTCGAAG ACTTCCTGAG GAAAACAAAC 
AGGGTGCTGA AGAATAAGGG CTTTGAGAAG GACATCACCG CCTCGATTGC CATGGCCATC 
ATTGAGCAGA AGATGGACCG GCATATGGAA ATGTGCTACA TTTTTGCTTC TGAGAAGAAG 
TGGGCCTTCT CAGACCAGTG GCTAGCCTGC CTGGTTAATA ACTGGGCTCT CTTCCGAGAG 
CCAGACTTGG TGTTAAAGCT GCTGGAAACA GTGATGGAAG TTACTGCGAC AGACAGAGCC 
ATCCCTGAAT CTCAGATCAA ACAAGTGATT GGCTTGATCC TGGAATGCTA CGCAGACCTC 
TCGCTGCCAG ATAAAAATAA AGTCCTCTCA GGCACCCTGC TTTCCTGGGG GCGGAAGGGT 
CTCTCTGAGA ACCTGTTGGC TTACTTGGAC GGGTTTCAGG AGGACCTCAA CACAACTTTT 
AACCAGCTTG CGCAGAGTGC CTCTGAACAG GGCTTAGCTA AAGCGGTGGC TTCTGTGGCC 
CGCCTGATAA TATTGCACCC GGAGATCACA GTGAAGAAAA TGTGCAGCAT GGCTGTGGTC 
AACCTTGGCA CCCACAAATT CCTGGCTCAG ATTCTCTCTG CCTTCCCTGC CCTTAGGTTC 
ACCGAAGAGC AGGATCCAAA TGCATCTACT ACCTTTGTGG TGTCATGCCT CAAAGAAACT 
GTTTGGACAA AGTTCTCTAC ACCTAAGGAA GAAAAGCAAT TTTTGGAGCT CCTGGGCTGC 
CTGGTGAGTC CTGTGAAGCC CCAGGGTATC CCGGTTGCTG CTCTTCTTGA GCCACATGAG 
GTGCTAAAGG AGTTTGTCCT GCCTTTCTTG ATGCTTGATG TCAAAGAGGT AGACCTCAGT 
TTGAAGATTT TCCTCCAGAC TCTTGAAGCA AATGCATGTT TAGAGGAATA CTGGCTCCAG 
ACCTGTTCTC CATTCCCCCT CATCTTCAGT TTGTGCCAGC TCCTGGACTG CTTCAGCAAA 
TACTGGCAGC TCCCCAAGGA GAAACGGTGC CTCTTGTTGG ATGGGAAGGA TCTGGTGGTC 
CATATCCTGG AGATCCTCTG TGAGATCGTA CTGGCTAATG CTGAGACATT TTCCCCAGAC 
ACCTGGATCA AGTCCCTGTC TTGGCTCCAC CGGAAGTTGG AGCAGCTCGA CTGGACAGTG 
CGCCTGAGGA TGAAGAACCT CTTTGAGGGC CACTTCAAAT GTGAGGTGCC AGCCACGCTT 
TTTGAGATCT GCAAGCTTTC TGAGGATGAA TGGACTTCCC AGGCCCACCC TGGGTATGGG 
CCAGGCACAG GGCTTCTTGC CTGGATGGAG TGCTGCTACA TTTCCAGCAG CATCTCTGAG 
CAGATGCTGT CCCTCTTGGT GGTAGATGTG GGTAACCCTG AAGAGGTCAG ATTGTTCAGC 
AAGGGCTTTC TGGTGGCCCT GGTACAAGTC ATGCCTTGGT GCAGCTCCCA AGAGTGGCAG 
TACCTTTACC AGCTGACCAG GAGACTATTG GAGAAACAAC TCCTACATGT CCCTTATAGC 
CTGGAATATA TTCAATTTGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCC GGAGCTCCAG 
CTGTCCGTAC TCTTGCTGAG AGTTTTCCAG TTTCTCTGCA GCCAGAGTTG TCGTAACTGG 
CTTCCTATAG AAGGCTGGAG CCATGTAGTC AGGCTTCTCT GTAACAGTCT GACCAATCTC 
CTGGACTCCG TTAGGATGAT ACAGTCAGTT GGCCCTTGGG CTCAAGGACA AGAACAGGAC 
CTGACCCAGG AAACCCTGTT TTTTTATACC CAGGTATTCT GTCACGTTCT GCACATCATG 
GCCATGCTCC CCCACGAGGT GTGCGAGCCG CTGTATGTCT TGGCCTTGGA AATCCTCACC 
TGCTATGAAA CCCTAAGCAA GGCTAATCCT TCTAGTAGTT CCCTGCTCCA GAAGGTAAAT 
GAGCAGCGCT TCTTAAAGTC CATCGCTGAG AACATTAGCC CTGAGGAACG GCGCCAAACC 
CTGCTGCAGA AGATCAGCAA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624970.1
CDS	136..3282
Translation	MTILHGGFLLAEQLFHPKALTELTKSDWEHVGQPIVEALREISSATTCSQPFAWKKKALIIIWAKVLQPYPVTPSDTDTRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTICHAELERFLEHMSVDTSSEDVAFFLSVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDEFSHPPKRFKSDPEVCPTMPLLAMLLTGLKQIQNRILCPGMKCCALANLADMLTVFALMEDDPQEVSATVYLDKLATVISVWNSDTQNPYYQQALVEKVKEAERDVSLTSLAKLPSETIFTGFEFMCSLLREWGKELQTMLNSSQGTSYDSYRLCDSLTSFSQNLKLYLDSTSLSKEERQVVSELAECVEDFLRKTNRVLKNKGFEKDITASIAMAIIEQKMDRHMEMCYIFASEKKWAFSDQWLACLVNNWALFREPDLVLKLLETVMEVTATDRAIPESQIKQVIGLILECYADLSLPDKNKVLSGTLLSWGRKGLSENLLAYLDGFQEDLNTTFNQLAQSASEQGLAKAVASVARLIILHPEITVKKMCSMAVVNLGTHKFLAQILSAFPALRFTEEQDPNASTTFVVSCLKETVWTKFSTPKEEKQFLELLGCLVSPVKPQGIPVAALLEPHEVLKEFVLPFLMLDVKEVDLSLKIFLQTLEANACLEEYWLQTCSPFPLIFSLCQLLDCFSKYWQLPKEKRCLLLDGKDLVVHILEILCEIVLANAETFSPDTWIKSLSWLHRKLEQLDWTVRLRMKNLFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECCYISSSISEQMLSLLVVDVGNPEEVRLFSKGFLVALVQVMPWCSSQEWQYLYQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAPELQLSVLLLRVFQFLCSQSCRNWLPIEGWSHVVRLLCNSLTNLLDSVRMIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMLPHEVCEPLYVLALEILTCYETLSKANPSSSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_005624913.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624913.3

ORF Insert Sequence:

ATGACTATTC TGCATGGGGG CTTTTTGCTT GCCGAGCAGC TCTTCCACCC CAAAGCACTG 
ACAGAACTGA CAAAGTCTGA CTGGGAACAT GTTGGGCAGC CCATTGTGGA GGCCCTAAGG 
GAGATCTCCT CCGCCACAAC ATGCTCCCAG CCCTTTGCCT GGAAGAAGAA AGCTCTGATC 
ATCATCTGGG CCAAGGTTCT TCAGCCCTAC CCTGTCACCC CTTCAGACAC TGACACTCGG 
TGGCAGGAAG ATGTGTTCTT CTCGGTAGGC AACATGATCC CTACCATCAA TCACACGGTC 
CTTTTTGAGC TGCTCAAGTC TCTGGAAGCT TCTGGACTCT TTATCCAGCT TCTGATGGCC 
CTGCCCACCA CCATCTGCCA TGCAGAACTA GAGCGCTTTT TGGAGCACAT GTCTGTTGAC 
ACTTCTTCAG AGGATGTGGC CTTTTTCCTC AGTGTCTGGT GGGAAATGAT GAAGCACAAA 
GGCAATCAGC AGGATCCTCT GCTCTCCCAG TTTAGAACAA TGGCCCATAA GTACTTGTCT 
TCTTCAGATG AGTTCTCCCA TCCTCCAAAG AGGTTTAAGT CAGACCCAGA GGTGTGTCCC 
ACTATGCCCC TGTTGGCCAT GCTGCTCACT GGGCTAAAGC AAATCCAAAA TAGAATCCTG 
TGCCCAGGGA TGAAATGCTG CGCATTAGCC AACTTAGCAG ACATGCTAAC AGTATTTGCA 
CTGATGGAGG ATGACCCCCA GGAAGTGTCT GCAACTGTGT ATCTGGACAA ACTGGCCACA 
GTGATCTCCG TGTGGAATTC GGACACCCAG AACCCGTATT ACCAACAGGC ACTAGTAGAG 
AAGGTAAAGG AGGCAGAACG GGACGTCAGC CTGACCTCCC TGGCCAAGCT TCCAAGCGAG 
ACGATTTTCA CTGGGTTTGA GTTCATGTGC AGCCTCCTGC GGGAGTGGGG GAAGGAGCTG 
CAGACCATGC TCAACAGCAG CCAGGGGACA AGTTATGACA GCTACCGGCT GTGTGACAGC 
CTGACTTCCT TCAGCCAGAA CTTGAAGCTC TACCTTGATT CTACCAGCTT GTCTAAGGAG 
GAGCGGCAGG TGGTCTCAGA GCTAGCAGAG TGTGTCGAAG ACTTCCTGAG GAAAACAAAC 
AGGGTGCTGA AGAATAAGGG CTTTGAGAAG GACATCACCG CCTCGATTGC CATGGCCATC 
ATTGAGCAGA AGATGGACCG GCATATGGAA ATGTGCTACA TTTTTGCTTC TGAGAAGAAG 
TGGGCCTTCT CAGACCAGTG GCTAGCCTGC CTGGTTAATA ACTGGGCTCT CTTCCGAGAG 
CCAGACTTGG TGTTAAAGCT GCTGGAAACA GTGATGGAAG TTACTGCGAC AGACAGAGCC 
ATCCCTGAAT CTCAGATCAA ACAAGTGATT GGCTTGATCC TGGAATGCTA CGCAGACCTC 
TCGCTGCCAG ATAAAAATAA AGTCCTCTCA GGCACCCTGC TTTCCTGGGG GCGGAAGGGT 
CTCTCTGAGA ACCTGTTGGC TTACTTGGAC GGGTTTCAGG AGGACCTCAA CACAACTTTT 
AACCAGCTTG CGCAGAGTGC CTCTGAACAG GGCTTAGCTA AAGCGGTGGC TTCTGTGGCC 
CGCCTGATAA TATTGCACCC GGAGATCACA GTGAAGAAAA TGTGCAGCAT GGCTGTGGTC 
AACCTTGGCA CCCACAAATT CCTGGCTCAG ATTCTCTCTG CCTTCCCTGC CCTTAGGTTC 
ACCGAAGAGC AGGATCCAAA TGCATCTACT ACCTTTGTGG TGTCATGCCT CAAAGAAACT 
GTTTGGACAA AGTTCTCTAC ACCTAAGGAA GAAAAGCAAT TTTTGGAGCT CCTGGGCTGC 
CTGGTGAGTC CTGTGAAGCC CCAGGGTATC CCGGTTGCTG CTCTTCTTGA GCCACATGAG 
GTGCTAAAGG AGTTTGTCCT GCCTTTCTTG ATGCTTGATG TCAAAGAGGT AGACCTCAGT 
TTGAAGATTT TCCTCCAGAC TCTTGAAGCA AATGCATGTT TAGAGGAATA CTGGCTCCAG 
ACCTGTTCTC CATTCCCCCT CATCTTCAGT TTGTGCCAGC TCCTGGACTG CTTCAGCAAA 
TACTGGCAGC TCCCCAAGGA GAAACGGTGC CTCTTGTTGG ATGGGAAGGA TCTGGTGGTC 
CATATCCTGG AGATCCTCTG TGAGATCGTA CTGGCTAATG CTGAGACATT TTCCCCAGAC 
ACCTGGATCA AGTCCCTGTC TTGGCTCCAC CGGAAGTTGG AGCAGCTCGA CTGGACAGTG 
CGCCTGAGGA TGAAGAACCT CTTTGAGGGC CACTTCAAAT GTGAGGTGCC AGCCACGCTT 
TTTGAGATCT GCAAGCTTTC TGAGGATGAA TGGACTTCCC AGGCCCACCC TGGGTATGGG 
CCAGGCACAG GGCTTCTTGC CTGGATGGAG TGCTGCTACA TTTCCAGCAG CATCTCTGAG 
CAGATGCTGT CCCTCTTGGT GGTAGATGTG GGTAACCCTG AAGAGGTCAG ATTGTTCAGC 
AAGGGCTTTC TGGTGGCCCT GGTACAAGTC ATGCCTTGGT GCAGCTCCCA AGAGTGGCAG 
TACCTTTACC AGCTGACCAG GAGACTATTG GAGAAACAAC TCCTACATGT CCCTTATAGC 
CTGGAATATA TTCAATTTGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCC GGAGCTCCAG 
CTGTCCGTAC TCTTGCTGAG AGTTTTCCAG TTTCTCTGCA GCCAGAGTTG TCGTAACTGG 
CTTCCTATAG AAGGCTGGAG CCATGTAGTC AGGCTTCTCT GTAACAGTCT GACCAATCTC 
CTGGACTCCG TTAGGATGAT ACAGTCAGTT GGCCCTTGGG CTCAAGGACA AGAACAGGAC 
CTGACCCAGG AAACCCTGTT TTTTTATACC CAGGTATTCT GTCACGTTCT GCACATCATG 
GCCATGCTCC CCCACGAGGT GTGCGAGCCG CTGTATGTCT TGGCCTTGGA AATCCTCACC 
TGCTATGAAA CCCTAAGCAA GGCTAATCCT TCTAGTAGTT CCCTGCTCCA GAAGGTAAAT 
GAGCAGCGCT TCTTAAAGTC CATCGCTGAG AACATTAGCC CTGAGGAACG GCGCCAAACC 
CTGCTGCAGA AGATCAGCAA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_005624913.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624970.1 Canis lupus familiaris gem (nuclear organelle) associated protein 4 (GEMIN4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe12299
Clone ID Related Accession (Same CDS sequence)	XM_005624913.3 , XM_005624913.2 , XM_005624915.3 , XM_005624914.2 , XM_005624915.2
Accession Version	XM_005624913.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	gem-associated protein 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624913.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTATTC TGCATGGGGG CTTTTTGCTT GCCGAGCAGC TCTTCCACCC CAAAGCACTG 
ACAGAACTGA CAAAGTCTGA CTGGGAACAT GTTGGGCAGC CCATTGTGGA GGCCCTAAGG 
GAGATCTCCT CCGCCACAAC ATGCTCCCAG CCCTTTGCCT GGAAGAAGAA AGCTCTGATC 
ATCATCTGGG CCAAGGTTCT TCAGCCCTAC CCTGTCACCC CTTCAGACAC TGACACTCGG 
TGGCAGGAAG ATGTGTTCTT CTCGGTAGGC AACATGATCC CTACCATCAA TCACACGGTC 
CTTTTTGAGC TGCTCAAGTC TCTGGAAGCT TCTGGACTCT TTATCCAGCT TCTGATGGCC 
CTGCCCACCA CCATCTGCCA TGCAGAACTA GAGCGCTTTT TGGAGCACAT GTCTGTTGAC 
ACTTCTTCAG AGGATGTGGC CTTTTTCCTC AGTGTCTGGT GGGAAATGAT GAAGCACAAA 
GGCAATCAGC AGGATCCTCT GCTCTCCCAG TTTAGAACAA TGGCCCATAA GTACTTGTCT 
TCTTCAGATG AGTTCTCCCA TCCTCCAAAG AGGTTTAAGT CAGACCCAGA GGTGTGTCCC 
ACTATGCCCC TGTTGGCCAT GCTGCTCACT GGGCTAAAGC AAATCCAAAA TAGAATCCTG 
TGCCCAGGGA TGAAATGCTG CGCATTAGCC AACTTAGCAG ACATGCTAAC AGTATTTGCA 
CTGATGGAGG ATGACCCCCA GGAAGTGTCT GCAACTGTGT ATCTGGACAA ACTGGCCACA 
GTGATCTCCG TGTGGAATTC GGACACCCAG AACCCGTATT ACCAACAGGC ACTAGTAGAG 
AAGGTAAAGG AGGCAGAACG GGACGTCAGC CTGACCTCCC TGGCCAAGCT TCCAAGCGAG 
ACGATTTTCA CTGGGTTTGA GTTCATGTGC AGCCTCCTGC GGGAGTGGGG GAAGGAGCTG 
CAGACCATGC TCAACAGCAG CCAGGGGACA AGTTATGACA GCTACCGGCT GTGTGACAGC 
CTGACTTCCT TCAGCCAGAA CTTGAAGCTC TACCTTGATT CTACCAGCTT GTCTAAGGAG 
GAGCGGCAGG TGGTCTCAGA GCTAGCAGAG TGTGTCGAAG ACTTCCTGAG GAAAACAAAC 
AGGGTGCTGA AGAATAAGGG CTTTGAGAAG GACATCACCG CCTCGATTGC CATGGCCATC 
ATTGAGCAGA AGATGGACCG GCATATGGAA ATGTGCTACA TTTTTGCTTC TGAGAAGAAG 
TGGGCCTTCT CAGACCAGTG GCTAGCCTGC CTGGTTAATA ACTGGGCTCT CTTCCGAGAG 
CCAGACTTGG TGTTAAAGCT GCTGGAAACA GTGATGGAAG TTACTGCGAC AGACAGAGCC 
ATCCCTGAAT CTCAGATCAA ACAAGTGATT GGCTTGATCC TGGAATGCTA CGCAGACCTC 
TCGCTGCCAG ATAAAAATAA AGTCCTCTCA GGCACCCTGC TTTCCTGGGG GCGGAAGGGT 
CTCTCTGAGA ACCTGTTGGC TTACTTGGAC GGGTTTCAGG AGGACCTCAA CACAACTTTT 
AACCAGCTTG CGCAGAGTGC CTCTGAACAG GGCTTAGCTA AAGCGGTGGC TTCTGTGGCC 
CGCCTGATAA TATTGCACCC GGAGATCACA GTGAAGAAAA TGTGCAGCAT GGCTGTGGTC 
AACCTTGGCA CCCACAAATT CCTGGCTCAG ATTCTCTCTG CCTTCCCTGC CCTTAGGTTC 
ACCGAAGAGC AGGATCCAAA TGCATCTACT ACCTTTGTGG TGTCATGCCT CAAAGAAACT 
GTTTGGACAA AGTTCTCTAC ACCTAAGGAA GAAAAGCAAT TTTTGGAGCT CCTGGGCTGC 
CTGGTGAGTC CTGTGAAGCC CCAGGGTATC CCGGTTGCTG CTCTTCTTGA GCCACATGAG 
GTGCTAAAGG AGTTTGTCCT GCCTTTCTTG ATGCTTGATG TCAAAGAGGT AGACCTCAGT 
TTGAAGATTT TCCTCCAGAC TCTTGAAGCA AATGCATGTT TAGAGGAATA CTGGCTCCAG 
ACCTGTTCTC CATTCCCCCT CATCTTCAGT TTGTGCCAGC TCCTGGACTG CTTCAGCAAA 
TACTGGCAGC TCCCCAAGGA GAAACGGTGC CTCTTGTTGG ATGGGAAGGA TCTGGTGGTC 
CATATCCTGG AGATCCTCTG TGAGATCGTA CTGGCTAATG CTGAGACATT TTCCCCAGAC 
ACCTGGATCA AGTCCCTGTC TTGGCTCCAC CGGAAGTTGG AGCAGCTCGA CTGGACAGTG 
CGCCTGAGGA TGAAGAACCT CTTTGAGGGC CACTTCAAAT GTGAGGTGCC AGCCACGCTT 
TTTGAGATCT GCAAGCTTTC TGAGGATGAA TGGACTTCCC AGGCCCACCC TGGGTATGGG 
CCAGGCACAG GGCTTCTTGC CTGGATGGAG TGCTGCTACA TTTCCAGCAG CATCTCTGAG 
CAGATGCTGT CCCTCTTGGT GGTAGATGTG GGTAACCCTG AAGAGGTCAG ATTGTTCAGC 
AAGGGCTTTC TGGTGGCCCT GGTACAAGTC ATGCCTTGGT GCAGCTCCCA AGAGTGGCAG 
TACCTTTACC AGCTGACCAG GAGACTATTG GAGAAACAAC TCCTACATGT CCCTTATAGC 
CTGGAATATA TTCAATTTGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCC GGAGCTCCAG 
CTGTCCGTAC TCTTGCTGAG AGTTTTCCAG TTTCTCTGCA GCCAGAGTTG TCGTAACTGG 
CTTCCTATAG AAGGCTGGAG CCATGTAGTC AGGCTTCTCT GTAACAGTCT GACCAATCTC 
CTGGACTCCG TTAGGATGAT ACAGTCAGTT GGCCCTTGGG CTCAAGGACA AGAACAGGAC 
CTGACCCAGG AAACCCTGTT TTTTTATACC CAGGTATTCT GTCACGTTCT GCACATCATG 
GCCATGCTCC CCCACGAGGT GTGCGAGCCG CTGTATGTCT TGGCCTTGGA AATCCTCACC 
TGCTATGAAA CCCTAAGCAA GGCTAATCCT TCTAGTAGTT CCCTGCTCCA GAAGGTAAAT 
GAGCAGCGCT TCTTAAAGTC CATCGCTGAG AACATTAGCC CTGAGGAACG GCGCCAAACC 
CTGCTGCAGA AGATCAGCAA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624970.1
CDS	168..3314
Translation	MTILHGGFLLAEQLFHPKALTELTKSDWEHVGQPIVEALREISSATTCSQPFAWKKKALIIIWAKVLQPYPVTPSDTDTRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTICHAELERFLEHMSVDTSSEDVAFFLSVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDEFSHPPKRFKSDPEVCPTMPLLAMLLTGLKQIQNRILCPGMKCCALANLADMLTVFALMEDDPQEVSATVYLDKLATVISVWNSDTQNPYYQQALVEKVKEAERDVSLTSLAKLPSETIFTGFEFMCSLLREWGKELQTMLNSSQGTSYDSYRLCDSLTSFSQNLKLYLDSTSLSKEERQVVSELAECVEDFLRKTNRVLKNKGFEKDITASIAMAIIEQKMDRHMEMCYIFASEKKWAFSDQWLACLVNNWALFREPDLVLKLLETVMEVTATDRAIPESQIKQVIGLILECYADLSLPDKNKVLSGTLLSWGRKGLSENLLAYLDGFQEDLNTTFNQLAQSASEQGLAKAVASVARLIILHPEITVKKMCSMAVVNLGTHKFLAQILSAFPALRFTEEQDPNASTTFVVSCLKETVWTKFSTPKEEKQFLELLGCLVSPVKPQGIPVAALLEPHEVLKEFVLPFLMLDVKEVDLSLKIFLQTLEANACLEEYWLQTCSPFPLIFSLCQLLDCFSKYWQLPKEKRCLLLDGKDLVVHILEILCEIVLANAETFSPDTWIKSLSWLHRKLEQLDWTVRLRMKNLFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECCYISSSISEQMLSLLVVDVGNPEEVRLFSKGFLVALVQVMPWCSSQEWQYLYQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAPELQLSVLLLRVFQFLCSQSCRNWLPIEGWSHVVRLLCNSLTNLLDSVRMIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMLPHEVCEPLYVLALEILTCYETLSKANPSSSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_005624913.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris gem (nuclear organelle) associated protein 4 (GEMIN4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624913.2

ORF Insert Sequence:

ATGACTATTC TGCATGGGGG CTTTTTGCTT GCCGAGCAGC TCTTCCACCC CAAAGCACTG 
ACAGAACTGA CAAAGTCTGA CTGGGAACAT GTTGGGCAGC CCATTGTGGA GGCCCTAAGG 
GAGATCTCCT CCGCCACAAC ATGCTCCCAG CCCTTTGCCT GGAAGAAGAA AGCTCTGATC 
ATCATCTGGG CCAAGGTTCT TCAGCCCTAC CCTGTCACCC CTTCAGACAC TGACACTCGG 
TGGCAGGAAG ATGTGTTCTT CTCGGTAGGC AACATGATCC CTACCATCAA TCACACGGTC 
CTTTTTGAGC TGCTCAAGTC TCTGGAAGCT TCTGGACTCT TTATCCAGCT TCTGATGGCC 
CTGCCCACCA CCATCTGCCA TGCAGAACTA GAGCGCTTTT TGGAGCACAT GTCTGTTGAC 
ACTTCTTCAG AGGATGTGGC CTTTTTCCTC AGTGTCTGGT GGGAAATGAT GAAGCACAAA 
GGCAATCAGC AGGATCCTCT GCTCTCCCAG TTTAGAACAA TGGCCCATAA GTACTTGTCT 
TCTTCAGATG AGTTCTCCCA TCCTCCAAAG AGGTTTAAGT CAGACCCAGA GGTGTGTCCC 
ACTATGCCCC TGTTGGCCAT GCTGCTCACT GGGCTAAAGC AAATCCAAAA TAGAATCCTG 
TGCCCAGGGA TGAAATGCTG CGCATTAGCC AACTTAGCAG ACATGCTAAC AGTATTTGCA 
CTGATGGAGG ATGACCCCCA GGAAGTGTCT GCAACTGTGT ATCTGGACAA ACTGGCCACA 
GTGATCTCCG TGTGGAATTC GGACACCCAG AACCCGTATT ACCAACAGGC ACTAGTAGAG 
AAGGTAAAGG AGGCAGAACG GGACGTCAGC CTGACCTCCC TGGCCAAGCT TCCAAGCGAG 
ACGATTTTCA CTGGGTTTGA GTTCATGTGC AGCCTCCTGC GGGAGTGGGG GAAGGAGCTG 
CAGACCATGC TCAACAGCAG CCAGGGGACA AGTTATGACA GCTACCGGCT GTGTGACAGC 
CTGACTTCCT TCAGCCAGAA CTTGAAGCTC TACCTTGATT CTACCAGCTT GTCTAAGGAG 
GAGCGGCAGG TGGTCTCAGA GCTAGCAGAG TGTGTCGAAG ACTTCCTGAG GAAAACAAAC 
AGGGTGCTGA AGAATAAGGG CTTTGAGAAG GACATCACCG CCTCGATTGC CATGGCCATC 
ATTGAGCAGA AGATGGACCG GCATATGGAA ATGTGCTACA TTTTTGCTTC TGAGAAGAAG 
TGGGCCTTCT CAGACCAGTG GCTAGCCTGC CTGGTTAATA ACTGGGCTCT CTTCCGAGAG 
CCAGACTTGG TGTTAAAGCT GCTGGAAACA GTGATGGAAG TTACTGCGAC AGACAGAGCC 
ATCCCTGAAT CTCAGATCAA ACAAGTGATT GGCTTGATCC TGGAATGCTA CGCAGACCTC 
TCGCTGCCAG ATAAAAATAA AGTCCTCTCA GGCACCCTGC TTTCCTGGGG GCGGAAGGGT 
CTCTCTGAGA ACCTGTTGGC TTACTTGGAC GGGTTTCAGG AGGACCTCAA CACAACTTTT 
AACCAGCTTG CGCAGAGTGC CTCTGAACAG GGCTTAGCTA AAGCGGTGGC TTCTGTGGCC 
CGCCTGATAA TATTGCACCC GGAGATCACA GTGAAGAAAA TGTGCAGCAT GGCTGTGGTC 
AACCTTGGCA CCCACAAATT CCTGGCTCAG ATTCTCTCTG CCTTCCCTGC CCTTAGGTTC 
ACCGAAGAGC AGGATCCAAA TGCATCTACT ACCTTTGTGG TGTCATGCCT CAAAGAAACT 
GTTTGGACAA AGTTCTCTAC ACCTAAGGAA GAAAAGCAAT TTTTGGAGCT CCTGGGCTGC 
CTGGTGAGTC CTGTGAAGCC CCAGGGTATC CCGGTTGCTG CTCTTCTTGA GCCACATGAG 
GTGCTAAAGG AGTTTGTCCT GCCTTTCTTG ATGCTTGATG TCAAAGAGGT AGACCTCAGT 
TTGAAGATTT TCCTCCAGAC TCTTGAAGCA AATGCATGTT TAGAGGAATA CTGGCTCCAG 
ACCTGTTCTC CATTCCCCCT CATCTTCAGT TTGTGCCAGC TCCTGGACTG CTTCAGCAAA 
TACTGGCAGC TCCCCAAGGA GAAACGGTGC CTCTTGTTGG ATGGGAAGGA TCTGGTGGTC 
CATATCCTGG AGATCCTCTG TGAGATCGTA CTGGCTAATG CTGAGACATT TTCCCCAGAC 
ACCTGGATCA AGTCCCTGTC TTGGCTCCAC CGGAAGTTGG AGCAGCTCGA CTGGACAGTG 
CGCCTGAGGA TGAAGAACCT CTTTGAGGGC CACTTCAAAT GTGAGGTGCC AGCCACGCTT 
TTTGAGATCT GCAAGCTTTC TGAGGATGAA TGGACTTCCC AGGCCCACCC TGGGTATGGG 
CCAGGCACAG GGCTTCTTGC CTGGATGGAG TGCTGCTACA TTTCCAGCAG CATCTCTGAG 
CAGATGCTGT CCCTCTTGGT GGTAGATGTG GGTAACCCTG AAGAGGTCAG ATTGTTCAGC 
AAGGGCTTTC TGGTGGCCCT GGTACAAGTC ATGCCTTGGT GCAGCTCCCA AGAGTGGCAG 
TACCTTTACC AGCTGACCAG GAGACTATTG GAGAAACAAC TCCTACATGT CCCTTATAGC 
CTGGAATATA TTCAATTTGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCC GGAGCTCCAG 
CTGTCCGTAC TCTTGCTGAG AGTTTTCCAG TTTCTCTGCA GCCAGAGTTG TCGTAACTGG 
CTTCCTATAG AAGGCTGGAG CCATGTAGTC AGGCTTCTCT GTAACAGTCT GACCAATCTC 
CTGGACTCCG TTAGGATGAT ACAGTCAGTT GGCCCTTGGG CTCAAGGACA AGAACAGGAC 
CTGACCCAGG AAACCCTGTT TTTTTATACC CAGGTATTCT GTCACGTTCT GCACATCATG 
GCCATGCTCC CCCACGAGGT GTGCGAGCCG CTGTATGTCT TGGCCTTGGA AATCCTCACC 
TGCTATGAAA CCCTAAGCAA GGCTAATCCT TCTAGTAGTT CCCTGCTCCA GAAGGTAAAT 
GAGCAGCGCT TCTTAAAGTC CATCGCTGAG AACATTAGCC CTGAGGAACG GCGCCAAACC 
CTGCTGCAGA AGATCAGCAA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_005624915.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624972.1 Canis lupus familiaris gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe12299
Clone ID Related Accession (Same CDS sequence)	XM_005624913.3 , XM_005624913.2 , XM_005624915.3 , XM_005624914.2 , XM_005624915.2
Accession Version	XM_005624915.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	gem-associated protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624915.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTATTC TGCATGGGGG CTTTTTGCTT GCCGAGCAGC TCTTCCACCC CAAAGCACTG 
ACAGAACTGA CAAAGTCTGA CTGGGAACAT GTTGGGCAGC CCATTGTGGA GGCCCTAAGG 
GAGATCTCCT CCGCCACAAC ATGCTCCCAG CCCTTTGCCT GGAAGAAGAA AGCTCTGATC 
ATCATCTGGG CCAAGGTTCT TCAGCCCTAC CCTGTCACCC CTTCAGACAC TGACACTCGG 
TGGCAGGAAG ATGTGTTCTT CTCGGTAGGC AACATGATCC CTACCATCAA TCACACGGTC 
CTTTTTGAGC TGCTCAAGTC TCTGGAAGCT TCTGGACTCT TTATCCAGCT TCTGATGGCC 
CTGCCCACCA CCATCTGCCA TGCAGAACTA GAGCGCTTTT TGGAGCACAT GTCTGTTGAC 
ACTTCTTCAG AGGATGTGGC CTTTTTCCTC AGTGTCTGGT GGGAAATGAT GAAGCACAAA 
GGCAATCAGC AGGATCCTCT GCTCTCCCAG TTTAGAACAA TGGCCCATAA GTACTTGTCT 
TCTTCAGATG AGTTCTCCCA TCCTCCAAAG AGGTTTAAGT CAGACCCAGA GGTGTGTCCC 
ACTATGCCCC TGTTGGCCAT GCTGCTCACT GGGCTAAAGC AAATCCAAAA TAGAATCCTG 
TGCCCAGGGA TGAAATGCTG CGCATTAGCC AACTTAGCAG ACATGCTAAC AGTATTTGCA 
CTGATGGAGG ATGACCCCCA GGAAGTGTCT GCAACTGTGT ATCTGGACAA ACTGGCCACA 
GTGATCTCCG TGTGGAATTC GGACACCCAG AACCCGTATT ACCAACAGGC ACTAGTAGAG 
AAGGTAAAGG AGGCAGAACG GGACGTCAGC CTGACCTCCC TGGCCAAGCT TCCAAGCGAG 
ACGATTTTCA CTGGGTTTGA GTTCATGTGC AGCCTCCTGC GGGAGTGGGG GAAGGAGCTG 
CAGACCATGC TCAACAGCAG CCAGGGGACA AGTTATGACA GCTACCGGCT GTGTGACAGC 
CTGACTTCCT TCAGCCAGAA CTTGAAGCTC TACCTTGATT CTACCAGCTT GTCTAAGGAG 
GAGCGGCAGG TGGTCTCAGA GCTAGCAGAG TGTGTCGAAG ACTTCCTGAG GAAAACAAAC 
AGGGTGCTGA AGAATAAGGG CTTTGAGAAG GACATCACCG CCTCGATTGC CATGGCCATC 
ATTGAGCAGA AGATGGACCG GCATATGGAA ATGTGCTACA TTTTTGCTTC TGAGAAGAAG 
TGGGCCTTCT CAGACCAGTG GCTAGCCTGC CTGGTTAATA ACTGGGCTCT CTTCCGAGAG 
CCAGACTTGG TGTTAAAGCT GCTGGAAACA GTGATGGAAG TTACTGCGAC AGACAGAGCC 
ATCCCTGAAT CTCAGATCAA ACAAGTGATT GGCTTGATCC TGGAATGCTA CGCAGACCTC 
TCGCTGCCAG ATAAAAATAA AGTCCTCTCA GGCACCCTGC TTTCCTGGGG GCGGAAGGGT 
CTCTCTGAGA ACCTGTTGGC TTACTTGGAC GGGTTTCAGG AGGACCTCAA CACAACTTTT 
AACCAGCTTG CGCAGAGTGC CTCTGAACAG GGCTTAGCTA AAGCGGTGGC TTCTGTGGCC 
CGCCTGATAA TATTGCACCC GGAGATCACA GTGAAGAAAA TGTGCAGCAT GGCTGTGGTC 
AACCTTGGCA CCCACAAATT CCTGGCTCAG ATTCTCTCTG CCTTCCCTGC CCTTAGGTTC 
ACCGAAGAGC AGGATCCAAA TGCATCTACT ACCTTTGTGG TGTCATGCCT CAAAGAAACT 
GTTTGGACAA AGTTCTCTAC ACCTAAGGAA GAAAAGCAAT TTTTGGAGCT CCTGGGCTGC 
CTGGTGAGTC CTGTGAAGCC CCAGGGTATC CCGGTTGCTG CTCTTCTTGA GCCACATGAG 
GTGCTAAAGG AGTTTGTCCT GCCTTTCTTG ATGCTTGATG TCAAAGAGGT AGACCTCAGT 
TTGAAGATTT TCCTCCAGAC TCTTGAAGCA AATGCATGTT TAGAGGAATA CTGGCTCCAG 
ACCTGTTCTC CATTCCCCCT CATCTTCAGT TTGTGCCAGC TCCTGGACTG CTTCAGCAAA 
TACTGGCAGC TCCCCAAGGA GAAACGGTGC CTCTTGTTGG ATGGGAAGGA TCTGGTGGTC 
CATATCCTGG AGATCCTCTG TGAGATCGTA CTGGCTAATG CTGAGACATT TTCCCCAGAC 
ACCTGGATCA AGTCCCTGTC TTGGCTCCAC CGGAAGTTGG AGCAGCTCGA CTGGACAGTG 
CGCCTGAGGA TGAAGAACCT CTTTGAGGGC CACTTCAAAT GTGAGGTGCC AGCCACGCTT 
TTTGAGATCT GCAAGCTTTC TGAGGATGAA TGGACTTCCC AGGCCCACCC TGGGTATGGG 
CCAGGCACAG GGCTTCTTGC CTGGATGGAG TGCTGCTACA TTTCCAGCAG CATCTCTGAG 
CAGATGCTGT CCCTCTTGGT GGTAGATGTG GGTAACCCTG AAGAGGTCAG ATTGTTCAGC 
AAGGGCTTTC TGGTGGCCCT GGTACAAGTC ATGCCTTGGT GCAGCTCCCA AGAGTGGCAG 
TACCTTTACC AGCTGACCAG GAGACTATTG GAGAAACAAC TCCTACATGT CCCTTATAGC 
CTGGAATATA TTCAATTTGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCC GGAGCTCCAG 
CTGTCCGTAC TCTTGCTGAG AGTTTTCCAG TTTCTCTGCA GCCAGAGTTG TCGTAACTGG 
CTTCCTATAG AAGGCTGGAG CCATGTAGTC AGGCTTCTCT GTAACAGTCT GACCAATCTC 
CTGGACTCCG TTAGGATGAT ACAGTCAGTT GGCCCTTGGG CTCAAGGACA AGAACAGGAC 
CTGACCCAGG AAACCCTGTT TTTTTATACC CAGGTATTCT GTCACGTTCT GCACATCATG 
GCCATGCTCC CCCACGAGGT GTGCGAGCCG CTGTATGTCT TGGCCTTGGA AATCCTCACC 
TGCTATGAAA CCCTAAGCAA GGCTAATCCT TCTAGTAGTT CCCTGCTCCA GAAGGTAAAT 
GAGCAGCGCT TCTTAAAGTC CATCGCTGAG AACATTAGCC CTGAGGAACG GCGCCAAACC 
CTGCTGCAGA AGATCAGCAA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624972.1
CDS	144..3290
Translation	MTILHGGFLLAEQLFHPKALTELTKSDWEHVGQPIVEALREISSATTCSQPFAWKKKALIIIWAKVLQPYPVTPSDTDTRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTICHAELERFLEHMSVDTSSEDVAFFLSVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDEFSHPPKRFKSDPEVCPTMPLLAMLLTGLKQIQNRILCPGMKCCALANLADMLTVFALMEDDPQEVSATVYLDKLATVISVWNSDTQNPYYQQALVEKVKEAERDVSLTSLAKLPSETIFTGFEFMCSLLREWGKELQTMLNSSQGTSYDSYRLCDSLTSFSQNLKLYLDSTSLSKEERQVVSELAECVEDFLRKTNRVLKNKGFEKDITASIAMAIIEQKMDRHMEMCYIFASEKKWAFSDQWLACLVNNWALFREPDLVLKLLETVMEVTATDRAIPESQIKQVIGLILECYADLSLPDKNKVLSGTLLSWGRKGLSENLLAYLDGFQEDLNTTFNQLAQSASEQGLAKAVASVARLIILHPEITVKKMCSMAVVNLGTHKFLAQILSAFPALRFTEEQDPNASTTFVVSCLKETVWTKFSTPKEEKQFLELLGCLVSPVKPQGIPVAALLEPHEVLKEFVLPFLMLDVKEVDLSLKIFLQTLEANACLEEYWLQTCSPFPLIFSLCQLLDCFSKYWQLPKEKRCLLLDGKDLVVHILEILCEIVLANAETFSPDTWIKSLSWLHRKLEQLDWTVRLRMKNLFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECCYISSSISEQMLSLLVVDVGNPEEVRLFSKGFLVALVQVMPWCSSQEWQYLYQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAPELQLSVLLLRVFQFLCSQSCRNWLPIEGWSHVVRLLCNSLTNLLDSVRMIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMLPHEVCEPLYVLALEILTCYETLSKANPSSSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_005624915.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624915.3

ORF Insert Sequence:

ATGACTATTC TGCATGGGGG CTTTTTGCTT GCCGAGCAGC TCTTCCACCC CAAAGCACTG 
ACAGAACTGA CAAAGTCTGA CTGGGAACAT GTTGGGCAGC CCATTGTGGA GGCCCTAAGG 
GAGATCTCCT CCGCCACAAC ATGCTCCCAG CCCTTTGCCT GGAAGAAGAA AGCTCTGATC 
ATCATCTGGG CCAAGGTTCT TCAGCCCTAC CCTGTCACCC CTTCAGACAC TGACACTCGG 
TGGCAGGAAG ATGTGTTCTT CTCGGTAGGC AACATGATCC CTACCATCAA TCACACGGTC 
CTTTTTGAGC TGCTCAAGTC TCTGGAAGCT TCTGGACTCT TTATCCAGCT TCTGATGGCC 
CTGCCCACCA CCATCTGCCA TGCAGAACTA GAGCGCTTTT TGGAGCACAT GTCTGTTGAC 
ACTTCTTCAG AGGATGTGGC CTTTTTCCTC AGTGTCTGGT GGGAAATGAT GAAGCACAAA 
GGCAATCAGC AGGATCCTCT GCTCTCCCAG TTTAGAACAA TGGCCCATAA GTACTTGTCT 
TCTTCAGATG AGTTCTCCCA TCCTCCAAAG AGGTTTAAGT CAGACCCAGA GGTGTGTCCC 
ACTATGCCCC TGTTGGCCAT GCTGCTCACT GGGCTAAAGC AAATCCAAAA TAGAATCCTG 
TGCCCAGGGA TGAAATGCTG CGCATTAGCC AACTTAGCAG ACATGCTAAC AGTATTTGCA 
CTGATGGAGG ATGACCCCCA GGAAGTGTCT GCAACTGTGT ATCTGGACAA ACTGGCCACA 
GTGATCTCCG TGTGGAATTC GGACACCCAG AACCCGTATT ACCAACAGGC ACTAGTAGAG 
AAGGTAAAGG AGGCAGAACG GGACGTCAGC CTGACCTCCC TGGCCAAGCT TCCAAGCGAG 
ACGATTTTCA CTGGGTTTGA GTTCATGTGC AGCCTCCTGC GGGAGTGGGG GAAGGAGCTG 
CAGACCATGC TCAACAGCAG CCAGGGGACA AGTTATGACA GCTACCGGCT GTGTGACAGC 
CTGACTTCCT TCAGCCAGAA CTTGAAGCTC TACCTTGATT CTACCAGCTT GTCTAAGGAG 
GAGCGGCAGG TGGTCTCAGA GCTAGCAGAG TGTGTCGAAG ACTTCCTGAG GAAAACAAAC 
AGGGTGCTGA AGAATAAGGG CTTTGAGAAG GACATCACCG CCTCGATTGC CATGGCCATC 
ATTGAGCAGA AGATGGACCG GCATATGGAA ATGTGCTACA TTTTTGCTTC TGAGAAGAAG 
TGGGCCTTCT CAGACCAGTG GCTAGCCTGC CTGGTTAATA ACTGGGCTCT CTTCCGAGAG 
CCAGACTTGG TGTTAAAGCT GCTGGAAACA GTGATGGAAG TTACTGCGAC AGACAGAGCC 
ATCCCTGAAT CTCAGATCAA ACAAGTGATT GGCTTGATCC TGGAATGCTA CGCAGACCTC 
TCGCTGCCAG ATAAAAATAA AGTCCTCTCA GGCACCCTGC TTTCCTGGGG GCGGAAGGGT 
CTCTCTGAGA ACCTGTTGGC TTACTTGGAC GGGTTTCAGG AGGACCTCAA CACAACTTTT 
AACCAGCTTG CGCAGAGTGC CTCTGAACAG GGCTTAGCTA AAGCGGTGGC TTCTGTGGCC 
CGCCTGATAA TATTGCACCC GGAGATCACA GTGAAGAAAA TGTGCAGCAT GGCTGTGGTC 
AACCTTGGCA CCCACAAATT CCTGGCTCAG ATTCTCTCTG CCTTCCCTGC CCTTAGGTTC 
ACCGAAGAGC AGGATCCAAA TGCATCTACT ACCTTTGTGG TGTCATGCCT CAAAGAAACT 
GTTTGGACAA AGTTCTCTAC ACCTAAGGAA GAAAAGCAAT TTTTGGAGCT CCTGGGCTGC 
CTGGTGAGTC CTGTGAAGCC CCAGGGTATC CCGGTTGCTG CTCTTCTTGA GCCACATGAG 
GTGCTAAAGG AGTTTGTCCT GCCTTTCTTG ATGCTTGATG TCAAAGAGGT AGACCTCAGT 
TTGAAGATTT TCCTCCAGAC TCTTGAAGCA AATGCATGTT TAGAGGAATA CTGGCTCCAG 
ACCTGTTCTC CATTCCCCCT CATCTTCAGT TTGTGCCAGC TCCTGGACTG CTTCAGCAAA 
TACTGGCAGC TCCCCAAGGA GAAACGGTGC CTCTTGTTGG ATGGGAAGGA TCTGGTGGTC 
CATATCCTGG AGATCCTCTG TGAGATCGTA CTGGCTAATG CTGAGACATT TTCCCCAGAC 
ACCTGGATCA AGTCCCTGTC TTGGCTCCAC CGGAAGTTGG AGCAGCTCGA CTGGACAGTG 
CGCCTGAGGA TGAAGAACCT CTTTGAGGGC CACTTCAAAT GTGAGGTGCC AGCCACGCTT 
TTTGAGATCT GCAAGCTTTC TGAGGATGAA TGGACTTCCC AGGCCCACCC TGGGTATGGG 
CCAGGCACAG GGCTTCTTGC CTGGATGGAG TGCTGCTACA TTTCCAGCAG CATCTCTGAG 
CAGATGCTGT CCCTCTTGGT GGTAGATGTG GGTAACCCTG AAGAGGTCAG ATTGTTCAGC 
AAGGGCTTTC TGGTGGCCCT GGTACAAGTC ATGCCTTGGT GCAGCTCCCA AGAGTGGCAG 
TACCTTTACC AGCTGACCAG GAGACTATTG GAGAAACAAC TCCTACATGT CCCTTATAGC 
CTGGAATATA TTCAATTTGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCC GGAGCTCCAG 
CTGTCCGTAC TCTTGCTGAG AGTTTTCCAG TTTCTCTGCA GCCAGAGTTG TCGTAACTGG 
CTTCCTATAG AAGGCTGGAG CCATGTAGTC AGGCTTCTCT GTAACAGTCT GACCAATCTC 
CTGGACTCCG TTAGGATGAT ACAGTCAGTT GGCCCTTGGG CTCAAGGACA AGAACAGGAC 
CTGACCCAGG AAACCCTGTT TTTTTATACC CAGGTATTCT GTCACGTTCT GCACATCATG 
GCCATGCTCC CCCACGAGGT GTGCGAGCCG CTGTATGTCT TGGCCTTGGA AATCCTCACC 
TGCTATGAAA CCCTAAGCAA GGCTAATCCT TCTAGTAGTT CCCTGCTCCA GAAGGTAAAT 
GAGCAGCGCT TCTTAAAGTC CATCGCTGAG AACATTAGCC CTGAGGAACG GCGCCAAACC 
CTGCTGCAGA AGATCAGCAA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_005624914.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624971.1 Canis lupus familiaris gem (nuclear organelle) associated protein 4 (GEMIN4), transcript variant X3, mRNA.

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CloneID	OCe12299
Clone ID Related Accession (Same CDS sequence)	XM_005624913.3 , XM_005624913.2 , XM_005624915.3 , XM_005624914.2 , XM_005624915.2
Accession Version	XM_005624914.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	gem-associated protein 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624914.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTATTC TGCATGGGGG CTTTTTGCTT GCCGAGCAGC TCTTCCACCC CAAAGCACTG 
ACAGAACTGA CAAAGTCTGA CTGGGAACAT GTTGGGCAGC CCATTGTGGA GGCCCTAAGG 
GAGATCTCCT CCGCCACAAC ATGCTCCCAG CCCTTTGCCT GGAAGAAGAA AGCTCTGATC 
ATCATCTGGG CCAAGGTTCT TCAGCCCTAC CCTGTCACCC CTTCAGACAC TGACACTCGG 
TGGCAGGAAG ATGTGTTCTT CTCGGTAGGC AACATGATCC CTACCATCAA TCACACGGTC 
CTTTTTGAGC TGCTCAAGTC TCTGGAAGCT TCTGGACTCT TTATCCAGCT TCTGATGGCC 
CTGCCCACCA CCATCTGCCA TGCAGAACTA GAGCGCTTTT TGGAGCACAT GTCTGTTGAC 
ACTTCTTCAG AGGATGTGGC CTTTTTCCTC AGTGTCTGGT GGGAAATGAT GAAGCACAAA 
GGCAATCAGC AGGATCCTCT GCTCTCCCAG TTTAGAACAA TGGCCCATAA GTACTTGTCT 
TCTTCAGATG AGTTCTCCCA TCCTCCAAAG AGGTTTAAGT CAGACCCAGA GGTGTGTCCC 
ACTATGCCCC TGTTGGCCAT GCTGCTCACT GGGCTAAAGC AAATCCAAAA TAGAATCCTG 
TGCCCAGGGA TGAAATGCTG CGCATTAGCC AACTTAGCAG ACATGCTAAC AGTATTTGCA 
CTGATGGAGG ATGACCCCCA GGAAGTGTCT GCAACTGTGT ATCTGGACAA ACTGGCCACA 
GTGATCTCCG TGTGGAATTC GGACACCCAG AACCCGTATT ACCAACAGGC ACTAGTAGAG 
AAGGTAAAGG AGGCAGAACG GGACGTCAGC CTGACCTCCC TGGCCAAGCT TCCAAGCGAG 
ACGATTTTCA CTGGGTTTGA GTTCATGTGC AGCCTCCTGC GGGAGTGGGG GAAGGAGCTG 
CAGACCATGC TCAACAGCAG CCAGGGGACA AGTTATGACA GCTACCGGCT GTGTGACAGC 
CTGACTTCCT TCAGCCAGAA CTTGAAGCTC TACCTTGATT CTACCAGCTT GTCTAAGGAG 
GAGCGGCAGG TGGTCTCAGA GCTAGCAGAG TGTGTCGAAG ACTTCCTGAG GAAAACAAAC 
AGGGTGCTGA AGAATAAGGG CTTTGAGAAG GACATCACCG CCTCGATTGC CATGGCCATC 
ATTGAGCAGA AGATGGACCG GCATATGGAA ATGTGCTACA TTTTTGCTTC TGAGAAGAAG 
TGGGCCTTCT CAGACCAGTG GCTAGCCTGC CTGGTTAATA ACTGGGCTCT CTTCCGAGAG 
CCAGACTTGG TGTTAAAGCT GCTGGAAACA GTGATGGAAG TTACTGCGAC AGACAGAGCC 
ATCCCTGAAT CTCAGATCAA ACAAGTGATT GGCTTGATCC TGGAATGCTA CGCAGACCTC 
TCGCTGCCAG ATAAAAATAA AGTCCTCTCA GGCACCCTGC TTTCCTGGGG GCGGAAGGGT 
CTCTCTGAGA ACCTGTTGGC TTACTTGGAC GGGTTTCAGG AGGACCTCAA CACAACTTTT 
AACCAGCTTG CGCAGAGTGC CTCTGAACAG GGCTTAGCTA AAGCGGTGGC TTCTGTGGCC 
CGCCTGATAA TATTGCACCC GGAGATCACA GTGAAGAAAA TGTGCAGCAT GGCTGTGGTC 
AACCTTGGCA CCCACAAATT CCTGGCTCAG ATTCTCTCTG CCTTCCCTGC CCTTAGGTTC 
ACCGAAGAGC AGGATCCAAA TGCATCTACT ACCTTTGTGG TGTCATGCCT CAAAGAAACT 
GTTTGGACAA AGTTCTCTAC ACCTAAGGAA GAAAAGCAAT TTTTGGAGCT CCTGGGCTGC 
CTGGTGAGTC CTGTGAAGCC CCAGGGTATC CCGGTTGCTG CTCTTCTTGA GCCACATGAG 
GTGCTAAAGG AGTTTGTCCT GCCTTTCTTG ATGCTTGATG TCAAAGAGGT AGACCTCAGT 
TTGAAGATTT TCCTCCAGAC TCTTGAAGCA AATGCATGTT TAGAGGAATA CTGGCTCCAG 
ACCTGTTCTC CATTCCCCCT CATCTTCAGT TTGTGCCAGC TCCTGGACTG CTTCAGCAAA 
TACTGGCAGC TCCCCAAGGA GAAACGGTGC CTCTTGTTGG ATGGGAAGGA TCTGGTGGTC 
CATATCCTGG AGATCCTCTG TGAGATCGTA CTGGCTAATG CTGAGACATT TTCCCCAGAC 
ACCTGGATCA AGTCCCTGTC TTGGCTCCAC CGGAAGTTGG AGCAGCTCGA CTGGACAGTG 
CGCCTGAGGA TGAAGAACCT CTTTGAGGGC CACTTCAAAT GTGAGGTGCC AGCCACGCTT 
TTTGAGATCT GCAAGCTTTC TGAGGATGAA TGGACTTCCC AGGCCCACCC TGGGTATGGG 
CCAGGCACAG GGCTTCTTGC CTGGATGGAG TGCTGCTACA TTTCCAGCAG CATCTCTGAG 
CAGATGCTGT CCCTCTTGGT GGTAGATGTG GGTAACCCTG AAGAGGTCAG ATTGTTCAGC 
AAGGGCTTTC TGGTGGCCCT GGTACAAGTC ATGCCTTGGT GCAGCTCCCA AGAGTGGCAG 
TACCTTTACC AGCTGACCAG GAGACTATTG GAGAAACAAC TCCTACATGT CCCTTATAGC 
CTGGAATATA TTCAATTTGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCC GGAGCTCCAG 
CTGTCCGTAC TCTTGCTGAG AGTTTTCCAG TTTCTCTGCA GCCAGAGTTG TCGTAACTGG 
CTTCCTATAG AAGGCTGGAG CCATGTAGTC AGGCTTCTCT GTAACAGTCT GACCAATCTC 
CTGGACTCCG TTAGGATGAT ACAGTCAGTT GGCCCTTGGG CTCAAGGACA AGAACAGGAC 
CTGACCCAGG AAACCCTGTT TTTTTATACC CAGGTATTCT GTCACGTTCT GCACATCATG 
GCCATGCTCC CCCACGAGGT GTGCGAGCCG CTGTATGTCT TGGCCTTGGA AATCCTCACC 
TGCTATGAAA CCCTAAGCAA GGCTAATCCT TCTAGTAGTT CCCTGCTCCA GAAGGTAAAT 
GAGCAGCGCT TCTTAAAGTC CATCGCTGAG AACATTAGCC CTGAGGAACG GCGCCAAACC 
CTGCTGCAGA AGATCAGCAA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624971.1
CDS	189..3335
Translation	MTILHGGFLLAEQLFHPKALTELTKSDWEHVGQPIVEALREISSATTCSQPFAWKKKALIIIWAKVLQPYPVTPSDTDTRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTICHAELERFLEHMSVDTSSEDVAFFLSVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDEFSHPPKRFKSDPEVCPTMPLLAMLLTGLKQIQNRILCPGMKCCALANLADMLTVFALMEDDPQEVSATVYLDKLATVISVWNSDTQNPYYQQALVEKVKEAERDVSLTSLAKLPSETIFTGFEFMCSLLREWGKELQTMLNSSQGTSYDSYRLCDSLTSFSQNLKLYLDSTSLSKEERQVVSELAECVEDFLRKTNRVLKNKGFEKDITASIAMAIIEQKMDRHMEMCYIFASEKKWAFSDQWLACLVNNWALFREPDLVLKLLETVMEVTATDRAIPESQIKQVIGLILECYADLSLPDKNKVLSGTLLSWGRKGLSENLLAYLDGFQEDLNTTFNQLAQSASEQGLAKAVASVARLIILHPEITVKKMCSMAVVNLGTHKFLAQILSAFPALRFTEEQDPNASTTFVVSCLKETVWTKFSTPKEEKQFLELLGCLVSPVKPQGIPVAALLEPHEVLKEFVLPFLMLDVKEVDLSLKIFLQTLEANACLEEYWLQTCSPFPLIFSLCQLLDCFSKYWQLPKEKRCLLLDGKDLVVHILEILCEIVLANAETFSPDTWIKSLSWLHRKLEQLDWTVRLRMKNLFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECCYISSSISEQMLSLLVVDVGNPEEVRLFSKGFLVALVQVMPWCSSQEWQYLYQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAPELQLSVLLLRVFQFLCSQSCRNWLPIEGWSHVVRLLCNSLTNLLDSVRMIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMLPHEVCEPLYVLALEILTCYETLSKANPSSSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_005624914.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris gem (nuclear organelle) associated protein 4 (GEMIN4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624914.2

ORF Insert Sequence:

ATGACTATTC TGCATGGGGG CTTTTTGCTT GCCGAGCAGC TCTTCCACCC CAAAGCACTG 
ACAGAACTGA CAAAGTCTGA CTGGGAACAT GTTGGGCAGC CCATTGTGGA GGCCCTAAGG 
GAGATCTCCT CCGCCACAAC ATGCTCCCAG CCCTTTGCCT GGAAGAAGAA AGCTCTGATC 
ATCATCTGGG CCAAGGTTCT TCAGCCCTAC CCTGTCACCC CTTCAGACAC TGACACTCGG 
TGGCAGGAAG ATGTGTTCTT CTCGGTAGGC AACATGATCC CTACCATCAA TCACACGGTC 
CTTTTTGAGC TGCTCAAGTC TCTGGAAGCT TCTGGACTCT TTATCCAGCT TCTGATGGCC 
CTGCCCACCA CCATCTGCCA TGCAGAACTA GAGCGCTTTT TGGAGCACAT GTCTGTTGAC 
ACTTCTTCAG AGGATGTGGC CTTTTTCCTC AGTGTCTGGT GGGAAATGAT GAAGCACAAA 
GGCAATCAGC AGGATCCTCT GCTCTCCCAG TTTAGAACAA TGGCCCATAA GTACTTGTCT 
TCTTCAGATG AGTTCTCCCA TCCTCCAAAG AGGTTTAAGT CAGACCCAGA GGTGTGTCCC 
ACTATGCCCC TGTTGGCCAT GCTGCTCACT GGGCTAAAGC AAATCCAAAA TAGAATCCTG 
TGCCCAGGGA TGAAATGCTG CGCATTAGCC AACTTAGCAG ACATGCTAAC AGTATTTGCA 
CTGATGGAGG ATGACCCCCA GGAAGTGTCT GCAACTGTGT ATCTGGACAA ACTGGCCACA 
GTGATCTCCG TGTGGAATTC GGACACCCAG AACCCGTATT ACCAACAGGC ACTAGTAGAG 
AAGGTAAAGG AGGCAGAACG GGACGTCAGC CTGACCTCCC TGGCCAAGCT TCCAAGCGAG 
ACGATTTTCA CTGGGTTTGA GTTCATGTGC AGCCTCCTGC GGGAGTGGGG GAAGGAGCTG 
CAGACCATGC TCAACAGCAG CCAGGGGACA AGTTATGACA GCTACCGGCT GTGTGACAGC 
CTGACTTCCT TCAGCCAGAA CTTGAAGCTC TACCTTGATT CTACCAGCTT GTCTAAGGAG 
GAGCGGCAGG TGGTCTCAGA GCTAGCAGAG TGTGTCGAAG ACTTCCTGAG GAAAACAAAC 
AGGGTGCTGA AGAATAAGGG CTTTGAGAAG GACATCACCG CCTCGATTGC CATGGCCATC 
ATTGAGCAGA AGATGGACCG GCATATGGAA ATGTGCTACA TTTTTGCTTC TGAGAAGAAG 
TGGGCCTTCT CAGACCAGTG GCTAGCCTGC CTGGTTAATA ACTGGGCTCT CTTCCGAGAG 
CCAGACTTGG TGTTAAAGCT GCTGGAAACA GTGATGGAAG TTACTGCGAC AGACAGAGCC 
ATCCCTGAAT CTCAGATCAA ACAAGTGATT GGCTTGATCC TGGAATGCTA CGCAGACCTC 
TCGCTGCCAG ATAAAAATAA AGTCCTCTCA GGCACCCTGC TTTCCTGGGG GCGGAAGGGT 
CTCTCTGAGA ACCTGTTGGC TTACTTGGAC GGGTTTCAGG AGGACCTCAA CACAACTTTT 
AACCAGCTTG CGCAGAGTGC CTCTGAACAG GGCTTAGCTA AAGCGGTGGC TTCTGTGGCC 
CGCCTGATAA TATTGCACCC GGAGATCACA GTGAAGAAAA TGTGCAGCAT GGCTGTGGTC 
AACCTTGGCA CCCACAAATT CCTGGCTCAG ATTCTCTCTG CCTTCCCTGC CCTTAGGTTC 
ACCGAAGAGC AGGATCCAAA TGCATCTACT ACCTTTGTGG TGTCATGCCT CAAAGAAACT 
GTTTGGACAA AGTTCTCTAC ACCTAAGGAA GAAAAGCAAT TTTTGGAGCT CCTGGGCTGC 
CTGGTGAGTC CTGTGAAGCC CCAGGGTATC CCGGTTGCTG CTCTTCTTGA GCCACATGAG 
GTGCTAAAGG AGTTTGTCCT GCCTTTCTTG ATGCTTGATG TCAAAGAGGT AGACCTCAGT 
TTGAAGATTT TCCTCCAGAC TCTTGAAGCA AATGCATGTT TAGAGGAATA CTGGCTCCAG 
ACCTGTTCTC CATTCCCCCT CATCTTCAGT TTGTGCCAGC TCCTGGACTG CTTCAGCAAA 
TACTGGCAGC TCCCCAAGGA GAAACGGTGC CTCTTGTTGG ATGGGAAGGA TCTGGTGGTC 
CATATCCTGG AGATCCTCTG TGAGATCGTA CTGGCTAATG CTGAGACATT TTCCCCAGAC 
ACCTGGATCA AGTCCCTGTC TTGGCTCCAC CGGAAGTTGG AGCAGCTCGA CTGGACAGTG 
CGCCTGAGGA TGAAGAACCT CTTTGAGGGC CACTTCAAAT GTGAGGTGCC AGCCACGCTT 
TTTGAGATCT GCAAGCTTTC TGAGGATGAA TGGACTTCCC AGGCCCACCC TGGGTATGGG 
CCAGGCACAG GGCTTCTTGC CTGGATGGAG TGCTGCTACA TTTCCAGCAG CATCTCTGAG 
CAGATGCTGT CCCTCTTGGT GGTAGATGTG GGTAACCCTG AAGAGGTCAG ATTGTTCAGC 
AAGGGCTTTC TGGTGGCCCT GGTACAAGTC ATGCCTTGGT GCAGCTCCCA AGAGTGGCAG 
TACCTTTACC AGCTGACCAG GAGACTATTG GAGAAACAAC TCCTACATGT CCCTTATAGC 
CTGGAATATA TTCAATTTGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCC GGAGCTCCAG 
CTGTCCGTAC TCTTGCTGAG AGTTTTCCAG TTTCTCTGCA GCCAGAGTTG TCGTAACTGG 
CTTCCTATAG AAGGCTGGAG CCATGTAGTC AGGCTTCTCT GTAACAGTCT GACCAATCTC 
CTGGACTCCG TTAGGATGAT ACAGTCAGTT GGCCCTTGGG CTCAAGGACA AGAACAGGAC 
CTGACCCAGG AAACCCTGTT TTTTTATACC CAGGTATTCT GTCACGTTCT GCACATCATG 
GCCATGCTCC CCCACGAGGT GTGCGAGCCG CTGTATGTCT TGGCCTTGGA AATCCTCACC 
TGCTATGAAA CCCTAAGCAA GGCTAATCCT TCTAGTAGTT CCCTGCTCCA GAAGGTAAAT 
GAGCAGCGCT TCTTAAAGTC CATCGCTGAG AACATTAGCC CTGAGGAACG GCGCCAAACC 
CTGCTGCAGA AGATCAGCAA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_005624915.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624972.1 Canis lupus familiaris gem (nuclear organelle) associated protein 4 (GEMIN4), transcript variant X4, mRNA.

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CloneID	OCe12299
Clone ID Related Accession (Same CDS sequence)	XM_005624913.3 , XM_005624913.2 , XM_005624915.3 , XM_005624914.2 , XM_005624915.2
Accession Version	XM_005624915.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	gem-associated protein 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624915.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTATTC TGCATGGGGG CTTTTTGCTT GCCGAGCAGC TCTTCCACCC CAAAGCACTG 
ACAGAACTGA CAAAGTCTGA CTGGGAACAT GTTGGGCAGC CCATTGTGGA GGCCCTAAGG 
GAGATCTCCT CCGCCACAAC ATGCTCCCAG CCCTTTGCCT GGAAGAAGAA AGCTCTGATC 
ATCATCTGGG CCAAGGTTCT TCAGCCCTAC CCTGTCACCC CTTCAGACAC TGACACTCGG 
TGGCAGGAAG ATGTGTTCTT CTCGGTAGGC AACATGATCC CTACCATCAA TCACACGGTC 
CTTTTTGAGC TGCTCAAGTC TCTGGAAGCT TCTGGACTCT TTATCCAGCT TCTGATGGCC 
CTGCCCACCA CCATCTGCCA TGCAGAACTA GAGCGCTTTT TGGAGCACAT GTCTGTTGAC 
ACTTCTTCAG AGGATGTGGC CTTTTTCCTC AGTGTCTGGT GGGAAATGAT GAAGCACAAA 
GGCAATCAGC AGGATCCTCT GCTCTCCCAG TTTAGAACAA TGGCCCATAA GTACTTGTCT 
TCTTCAGATG AGTTCTCCCA TCCTCCAAAG AGGTTTAAGT CAGACCCAGA GGTGTGTCCC 
ACTATGCCCC TGTTGGCCAT GCTGCTCACT GGGCTAAAGC AAATCCAAAA TAGAATCCTG 
TGCCCAGGGA TGAAATGCTG CGCATTAGCC AACTTAGCAG ACATGCTAAC AGTATTTGCA 
CTGATGGAGG ATGACCCCCA GGAAGTGTCT GCAACTGTGT ATCTGGACAA ACTGGCCACA 
GTGATCTCCG TGTGGAATTC GGACACCCAG AACCCGTATT ACCAACAGGC ACTAGTAGAG 
AAGGTAAAGG AGGCAGAACG GGACGTCAGC CTGACCTCCC TGGCCAAGCT TCCAAGCGAG 
ACGATTTTCA CTGGGTTTGA GTTCATGTGC AGCCTCCTGC GGGAGTGGGG GAAGGAGCTG 
CAGACCATGC TCAACAGCAG CCAGGGGACA AGTTATGACA GCTACCGGCT GTGTGACAGC 
CTGACTTCCT TCAGCCAGAA CTTGAAGCTC TACCTTGATT CTACCAGCTT GTCTAAGGAG 
GAGCGGCAGG TGGTCTCAGA GCTAGCAGAG TGTGTCGAAG ACTTCCTGAG GAAAACAAAC 
AGGGTGCTGA AGAATAAGGG CTTTGAGAAG GACATCACCG CCTCGATTGC CATGGCCATC 
ATTGAGCAGA AGATGGACCG GCATATGGAA ATGTGCTACA TTTTTGCTTC TGAGAAGAAG 
TGGGCCTTCT CAGACCAGTG GCTAGCCTGC CTGGTTAATA ACTGGGCTCT CTTCCGAGAG 
CCAGACTTGG TGTTAAAGCT GCTGGAAACA GTGATGGAAG TTACTGCGAC AGACAGAGCC 
ATCCCTGAAT CTCAGATCAA ACAAGTGATT GGCTTGATCC TGGAATGCTA CGCAGACCTC 
TCGCTGCCAG ATAAAAATAA AGTCCTCTCA GGCACCCTGC TTTCCTGGGG GCGGAAGGGT 
CTCTCTGAGA ACCTGTTGGC TTACTTGGAC GGGTTTCAGG AGGACCTCAA CACAACTTTT 
AACCAGCTTG CGCAGAGTGC CTCTGAACAG GGCTTAGCTA AAGCGGTGGC TTCTGTGGCC 
CGCCTGATAA TATTGCACCC GGAGATCACA GTGAAGAAAA TGTGCAGCAT GGCTGTGGTC 
AACCTTGGCA CCCACAAATT CCTGGCTCAG ATTCTCTCTG CCTTCCCTGC CCTTAGGTTC 
ACCGAAGAGC AGGATCCAAA TGCATCTACT ACCTTTGTGG TGTCATGCCT CAAAGAAACT 
GTTTGGACAA AGTTCTCTAC ACCTAAGGAA GAAAAGCAAT TTTTGGAGCT CCTGGGCTGC 
CTGGTGAGTC CTGTGAAGCC CCAGGGTATC CCGGTTGCTG CTCTTCTTGA GCCACATGAG 
GTGCTAAAGG AGTTTGTCCT GCCTTTCTTG ATGCTTGATG TCAAAGAGGT AGACCTCAGT 
TTGAAGATTT TCCTCCAGAC TCTTGAAGCA AATGCATGTT TAGAGGAATA CTGGCTCCAG 
ACCTGTTCTC CATTCCCCCT CATCTTCAGT TTGTGCCAGC TCCTGGACTG CTTCAGCAAA 
TACTGGCAGC TCCCCAAGGA GAAACGGTGC CTCTTGTTGG ATGGGAAGGA TCTGGTGGTC 
CATATCCTGG AGATCCTCTG TGAGATCGTA CTGGCTAATG CTGAGACATT TTCCCCAGAC 
ACCTGGATCA AGTCCCTGTC TTGGCTCCAC CGGAAGTTGG AGCAGCTCGA CTGGACAGTG 
CGCCTGAGGA TGAAGAACCT CTTTGAGGGC CACTTCAAAT GTGAGGTGCC AGCCACGCTT 
TTTGAGATCT GCAAGCTTTC TGAGGATGAA TGGACTTCCC AGGCCCACCC TGGGTATGGG 
CCAGGCACAG GGCTTCTTGC CTGGATGGAG TGCTGCTACA TTTCCAGCAG CATCTCTGAG 
CAGATGCTGT CCCTCTTGGT GGTAGATGTG GGTAACCCTG AAGAGGTCAG ATTGTTCAGC 
AAGGGCTTTC TGGTGGCCCT GGTACAAGTC ATGCCTTGGT GCAGCTCCCA AGAGTGGCAG 
TACCTTTACC AGCTGACCAG GAGACTATTG GAGAAACAAC TCCTACATGT CCCTTATAGC 
CTGGAATATA TTCAATTTGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCC GGAGCTCCAG 
CTGTCCGTAC TCTTGCTGAG AGTTTTCCAG TTTCTCTGCA GCCAGAGTTG TCGTAACTGG 
CTTCCTATAG AAGGCTGGAG CCATGTAGTC AGGCTTCTCT GTAACAGTCT GACCAATCTC 
CTGGACTCCG TTAGGATGAT ACAGTCAGTT GGCCCTTGGG CTCAAGGACA AGAACAGGAC 
CTGACCCAGG AAACCCTGTT TTTTTATACC CAGGTATTCT GTCACGTTCT GCACATCATG 
GCCATGCTCC CCCACGAGGT GTGCGAGCCG CTGTATGTCT TGGCCTTGGA AATCCTCACC 
TGCTATGAAA CCCTAAGCAA GGCTAATCCT TCTAGTAGTT CCCTGCTCCA GAAGGTAAAT 
GAGCAGCGCT TCTTAAAGTC CATCGCTGAG AACATTAGCC CTGAGGAACG GCGCCAAACC 
CTGCTGCAGA AGATCAGCAA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624972.1
CDS	264..3410
Translation	MTILHGGFLLAEQLFHPKALTELTKSDWEHVGQPIVEALREISSATTCSQPFAWKKKALIIIWAKVLQPYPVTPSDTDTRWQEDVFFSVGNMIPTINHTVLFELLKSLEASGLFIQLLMALPTTICHAELERFLEHMSVDTSSEDVAFFLSVWWEMMKHKGNQQDPLLSQFRTMAHKYLSSSDEFSHPPKRFKSDPEVCPTMPLLAMLLTGLKQIQNRILCPGMKCCALANLADMLTVFALMEDDPQEVSATVYLDKLATVISVWNSDTQNPYYQQALVEKVKEAERDVSLTSLAKLPSETIFTGFEFMCSLLREWGKELQTMLNSSQGTSYDSYRLCDSLTSFSQNLKLYLDSTSLSKEERQVVSELAECVEDFLRKTNRVLKNKGFEKDITASIAMAIIEQKMDRHMEMCYIFASEKKWAFSDQWLACLVNNWALFREPDLVLKLLETVMEVTATDRAIPESQIKQVIGLILECYADLSLPDKNKVLSGTLLSWGRKGLSENLLAYLDGFQEDLNTTFNQLAQSASEQGLAKAVASVARLIILHPEITVKKMCSMAVVNLGTHKFLAQILSAFPALRFTEEQDPNASTTFVVSCLKETVWTKFSTPKEEKQFLELLGCLVSPVKPQGIPVAALLEPHEVLKEFVLPFLMLDVKEVDLSLKIFLQTLEANACLEEYWLQTCSPFPLIFSLCQLLDCFSKYWQLPKEKRCLLLDGKDLVVHILEILCEIVLANAETFSPDTWIKSLSWLHRKLEQLDWTVRLRMKNLFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGPGTGLLAWMECCYISSSISEQMLSLLVVDVGNPEEVRLFSKGFLVALVQVMPWCSSQEWQYLYQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAPELQLSVLLLRVFQFLCSQSCRNWLPIEGWSHVVRLLCNSLTNLLDSVRMIQSVGPWAQGQEQDLTQETLFFYTQVFCHVLHIMAMLPHEVCEPLYVLALEILTCYETLSKANPSSSSLLQKVNEQRFLKSIAENISPEERRQTLLQKISNF

Target ORF information:

RefSeq Version	XM_005624915.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris gem (nuclear organelle) associated protein 4 (GEMIN4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624915.2

ORF Insert Sequence:

ATGACTATTC TGCATGGGGG CTTTTTGCTT GCCGAGCAGC TCTTCCACCC CAAAGCACTG 
ACAGAACTGA CAAAGTCTGA CTGGGAACAT GTTGGGCAGC CCATTGTGGA GGCCCTAAGG 
GAGATCTCCT CCGCCACAAC ATGCTCCCAG CCCTTTGCCT GGAAGAAGAA AGCTCTGATC 
ATCATCTGGG CCAAGGTTCT TCAGCCCTAC CCTGTCACCC CTTCAGACAC TGACACTCGG 
TGGCAGGAAG ATGTGTTCTT CTCGGTAGGC AACATGATCC CTACCATCAA TCACACGGTC 
CTTTTTGAGC TGCTCAAGTC TCTGGAAGCT TCTGGACTCT TTATCCAGCT TCTGATGGCC 
CTGCCCACCA CCATCTGCCA TGCAGAACTA GAGCGCTTTT TGGAGCACAT GTCTGTTGAC 
ACTTCTTCAG AGGATGTGGC CTTTTTCCTC AGTGTCTGGT GGGAAATGAT GAAGCACAAA 
GGCAATCAGC AGGATCCTCT GCTCTCCCAG TTTAGAACAA TGGCCCATAA GTACTTGTCT 
TCTTCAGATG AGTTCTCCCA TCCTCCAAAG AGGTTTAAGT CAGACCCAGA GGTGTGTCCC 
ACTATGCCCC TGTTGGCCAT GCTGCTCACT GGGCTAAAGC AAATCCAAAA TAGAATCCTG 
TGCCCAGGGA TGAAATGCTG CGCATTAGCC AACTTAGCAG ACATGCTAAC AGTATTTGCA 
CTGATGGAGG ATGACCCCCA GGAAGTGTCT GCAACTGTGT ATCTGGACAA ACTGGCCACA 
GTGATCTCCG TGTGGAATTC GGACACCCAG AACCCGTATT ACCAACAGGC ACTAGTAGAG 
AAGGTAAAGG AGGCAGAACG GGACGTCAGC CTGACCTCCC TGGCCAAGCT TCCAAGCGAG 
ACGATTTTCA CTGGGTTTGA GTTCATGTGC AGCCTCCTGC GGGAGTGGGG GAAGGAGCTG 
CAGACCATGC TCAACAGCAG CCAGGGGACA AGTTATGACA GCTACCGGCT GTGTGACAGC 
CTGACTTCCT TCAGCCAGAA CTTGAAGCTC TACCTTGATT CTACCAGCTT GTCTAAGGAG 
GAGCGGCAGG TGGTCTCAGA GCTAGCAGAG TGTGTCGAAG ACTTCCTGAG GAAAACAAAC 
AGGGTGCTGA AGAATAAGGG CTTTGAGAAG GACATCACCG CCTCGATTGC CATGGCCATC 
ATTGAGCAGA AGATGGACCG GCATATGGAA ATGTGCTACA TTTTTGCTTC TGAGAAGAAG 
TGGGCCTTCT CAGACCAGTG GCTAGCCTGC CTGGTTAATA ACTGGGCTCT CTTCCGAGAG 
CCAGACTTGG TGTTAAAGCT GCTGGAAACA GTGATGGAAG TTACTGCGAC AGACAGAGCC 
ATCCCTGAAT CTCAGATCAA ACAAGTGATT GGCTTGATCC TGGAATGCTA CGCAGACCTC 
TCGCTGCCAG ATAAAAATAA AGTCCTCTCA GGCACCCTGC TTTCCTGGGG GCGGAAGGGT 
CTCTCTGAGA ACCTGTTGGC TTACTTGGAC GGGTTTCAGG AGGACCTCAA CACAACTTTT 
AACCAGCTTG CGCAGAGTGC CTCTGAACAG GGCTTAGCTA AAGCGGTGGC TTCTGTGGCC 
CGCCTGATAA TATTGCACCC GGAGATCACA GTGAAGAAAA TGTGCAGCAT GGCTGTGGTC 
AACCTTGGCA CCCACAAATT CCTGGCTCAG ATTCTCTCTG CCTTCCCTGC CCTTAGGTTC 
ACCGAAGAGC AGGATCCAAA TGCATCTACT ACCTTTGTGG TGTCATGCCT CAAAGAAACT 
GTTTGGACAA AGTTCTCTAC ACCTAAGGAA GAAAAGCAAT TTTTGGAGCT CCTGGGCTGC 
CTGGTGAGTC CTGTGAAGCC CCAGGGTATC CCGGTTGCTG CTCTTCTTGA GCCACATGAG 
GTGCTAAAGG AGTTTGTCCT GCCTTTCTTG ATGCTTGATG TCAAAGAGGT AGACCTCAGT 
TTGAAGATTT TCCTCCAGAC TCTTGAAGCA AATGCATGTT TAGAGGAATA CTGGCTCCAG 
ACCTGTTCTC CATTCCCCCT CATCTTCAGT TTGTGCCAGC TCCTGGACTG CTTCAGCAAA 
TACTGGCAGC TCCCCAAGGA GAAACGGTGC CTCTTGTTGG ATGGGAAGGA TCTGGTGGTC 
CATATCCTGG AGATCCTCTG TGAGATCGTA CTGGCTAATG CTGAGACATT TTCCCCAGAC 
ACCTGGATCA AGTCCCTGTC TTGGCTCCAC CGGAAGTTGG AGCAGCTCGA CTGGACAGTG 
CGCCTGAGGA TGAAGAACCT CTTTGAGGGC CACTTCAAAT GTGAGGTGCC AGCCACGCTT 
TTTGAGATCT GCAAGCTTTC TGAGGATGAA TGGACTTCCC AGGCCCACCC TGGGTATGGG 
CCAGGCACAG GGCTTCTTGC CTGGATGGAG TGCTGCTACA TTTCCAGCAG CATCTCTGAG 
CAGATGCTGT CCCTCTTGGT GGTAGATGTG GGTAACCCTG AAGAGGTCAG ATTGTTCAGC 
AAGGGCTTTC TGGTGGCCCT GGTACAAGTC ATGCCTTGGT GCAGCTCCCA AGAGTGGCAG 
TACCTTTACC AGCTGACCAG GAGACTATTG GAGAAACAAC TCCTACATGT CCCTTATAGC 
CTGGAATATA TTCAATTTGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCC GGAGCTCCAG 
CTGTCCGTAC TCTTGCTGAG AGTTTTCCAG TTTCTCTGCA GCCAGAGTTG TCGTAACTGG 
CTTCCTATAG AAGGCTGGAG CCATGTAGTC AGGCTTCTCT GTAACAGTCT GACCAATCTC 
CTGGACTCCG TTAGGATGAT ACAGTCAGTT GGCCCTTGGG CTCAAGGACA AGAACAGGAC 
CTGACCCAGG AAACCCTGTT TTTTTATACC CAGGTATTCT GTCACGTTCT GCACATCATG 
GCCATGCTCC CCCACGAGGT GTGCGAGCCG CTGTATGTCT TGGCCTTGGA AATCCTCACC 
TGCTATGAAA CCCTAAGCAA GGCTAATCCT TCTAGTAGTT CCCTGCTCCA GAAGGTAAAT 
GAGCAGCGCT TCTTAAAGTC CATCGCTGAG AACATTAGCC CTGAGGAACG GCGCCAAACC 
CTGCTGCAGA AGATCAGCAA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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GEMIN4 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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