gemin4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	gemin4
Entrez Gene ID	100379790
Full Name	gem nuclear organelle associated protein 4
General protein information	Preferred Names gem nuclear organelle associated protein 4 Names gem-associated protein 4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004911680.3	XP_004911737.1	gem-associated protein 4
XM_002934341.5	XP_002934387.1	gem-associated protein 4
XM_004911680.4	XP_004911737.1	gem-associated protein 4
XM_002934341.4	XP_002934387.1	gem-associated protein 4
XM_004911679.4	XP_004911736.1	gem-associated protein 4
XM_012957516.2	XP_012812970.1	gem-associated protein 4
XM_012957516.3	XP_012812970.1	gem-associated protein 4
XM_004911679.3	XP_004911736.1	gem-associated protein 4

Pathways from BioSystems

Homologies

	GEMIN4	XP_001084635.1
Gallus gallus (chicken)	GEMIN4	NP_001012628.1
Xenopus tropicalis (tropical clawed frog)	gemin4	XP_004911736.1
Canis lupus familiaris (dog)	GEMIN4	XP_853922.2
Rattus norvegicus (Norway rat)	Gemin4	NP_001102507.1
Danio rerio (zebrafish)	gemin4	NP_001018154.1
Mus musculus (house mouse)	Gemin4	NP_796341.2
Homo sapiens (human)	GEMIN4	NP_056536.2
Pan troglodytes (chimpanzee)	GEMIN4	XP_511244.2
Bos taurus (cattle)	GEMIN4	XP_002695725.1

Gene Ontology

The following gemin4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gemin4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa52402	XM_004911679.4 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa52402	XM_012957516.3 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa52402	XM_004911680.4 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa52402	XM_002934341.5 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa05636	XM_004911679.3 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa05636	XM_002934341.4 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa05636	XM_004911680.3 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa05636	XM_012957516.2 Latest version!	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gemin4 ( XM_002934341.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934387.1 Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa52402
Clone ID Related Accession (Same CDS sequence)	XM_002934341.5 , XM_004911680.4 , XM_004911679.4 , XM_012957516.3
Accession Version	XM_002934341.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934341.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT CTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCCGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934387.1
CDS	195..3401
Translation	MDFGSWNVCEQTAVLQGAFLLAEKLCHPKTLLEVKKSDWPLIQKPITEAFKEISSGLPPQHQEWRRRAIAILWARTLCCTPDLTSVSHFDIDRKWKEDVFFPVENMIPKISQTVLFELLKTMKAADICAELLLALPVGIWAEGATSLLQHICEETSVEDVDFFLDVWWEVMKSKSDEQNETLVLFSSVVSQYLPKSSDEPSQSAKRFKSDPEQTLNQADNILAVFLEGLHKMKQCVATNKLRCFTIANLAEMLCSSAFLEKESELLPIKSYLEQFAVVLSFCEKGSRDVDMIRVAERMVQSGSTSSRFRLLRGAQHFGLSLLKDLLFQWGEELIHYLNNNKVVGYVCYRMRHSLASLHKILRTLEDSGTYPEEQRGDVLELAHCITSFQEKTTQVQHGCPSNANEMMAFIAMKIIENKMDRFKEVCSVFASEVSWAFSNDWLNCLEKNKAHFQDPAMVLKLLDLIVTVTALQSNVDIGSVRKGADVTLSIFSELSLSEKNEVLKEVLSLWGRKGLSPSIKAFTDSFQDELNITFNQISQSLSDYSAAQAVSKVSKLALLNPQAVVKKACHFAVVNVGAHTFLAKILSSLPALCFKCPNNSEKTVSLLCECLMETCWGKLSSDKEERQFLALLAFLMESSEVNDKDSSIQLLQPAEVIQTFVLPYIFDEYANVALCLHILHKAFNVQNPEDESGKHWVLSCSPFPLIMSLCKLLNSYTKCWQLSENSHCLTLGSKELIIDILTLIINAVMPEAGNNSETWNKSLFWLHKKMENLDWVVCLRLKPIFGKHFKNEVPSSLFDVGKLSEREWVPLQLPEYGPGTGLMAWLECCCISAEMKNQMLSLLSVNSKDPEEVNLFSKGFLVAMIQVLPWCSSTECKLVAEVVRHLLKRRLLHVPYTLEYVQHMPLLNLRPFAYNLQFSVLLLRGFQLLCSSSCSDWLPFEGHKHIARLYTNCIFDILEAVKQQISGNKNETQTERKEQDYVQEASFFYMQVFCHVLHIIAMMPDNTCEPLYVLSLEILSLYETLRASDTSTDSFLRRANERHFLKSITENVSDEHHRTTLMQKINKL

Target ORF information:

RefSeq Version	XM_002934341.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934341.5

ORF Insert Sequence:

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT CTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCCGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin4 ( XM_004911680.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911737.1 Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa52402
Clone ID Related Accession (Same CDS sequence)	XM_002934341.5 , XM_004911680.4 , XM_004911679.4 , XM_012957516.3
Accession Version	XM_004911680.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911680.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT CTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCCGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911737.1
CDS	231..3437
Translation	MDFGSWNVCEQTAVLQGAFLLAEKLCHPKTLLEVKKSDWPLIQKPITEAFKEISSGLPPQHQEWRRRAIAILWARTLCCTPDLTSVSHFDIDRKWKEDVFFPVENMIPKISQTVLFELLKTMKAADICAELLLALPVGIWAEGATSLLQHICEETSVEDVDFFLDVWWEVMKSKSDEQNETLVLFSSVVSQYLPKSSDEPSQSAKRFKSDPEQTLNQADNILAVFLEGLHKMKQCVATNKLRCFTIANLAEMLCSSAFLEKESELLPIKSYLEQFAVVLSFCEKGSRDVDMIRVAERMVQSGSTSSRFRLLRGAQHFGLSLLKDLLFQWGEELIHYLNNNKVVGYVCYRMRHSLASLHKILRTLEDSGTYPEEQRGDVLELAHCITSFQEKTTQVQHGCPSNANEMMAFIAMKIIENKMDRFKEVCSVFASEVSWAFSNDWLNCLEKNKAHFQDPAMVLKLLDLIVTVTALQSNVDIGSVRKGADVTLSIFSELSLSEKNEVLKEVLSLWGRKGLSPSIKAFTDSFQDELNITFNQISQSLSDYSAAQAVSKVSKLALLNPQAVVKKACHFAVVNVGAHTFLAKILSSLPALCFKCPNNSEKTVSLLCECLMETCWGKLSSDKEERQFLALLAFLMESSEVNDKDSSIQLLQPAEVIQTFVLPYIFDEYANVALCLHILHKAFNVQNPEDESGKHWVLSCSPFPLIMSLCKLLNSYTKCWQLSENSHCLTLGSKELIIDILTLIINAVMPEAGNNSETWNKSLFWLHKKMENLDWVVCLRLKPIFGKHFKNEVPSSLFDVGKLSEREWVPLQLPEYGPGTGLMAWLECCCISAEMKNQMLSLLSVNSKDPEEVNLFSKGFLVAMIQVLPWCSSTECKLVAEVVRHLLKRRLLHVPYTLEYVQHMPLLNLRPFAYNLQFSVLLLRGFQLLCSSSCSDWLPFEGHKHIARLYTNCIFDILEAVKQQISGNKNETQTERKEQDYVQEASFFYMQVFCHVLHIIAMMPDNTCEPLYVLSLEILSLYETLRASDTSTDSFLRRANERHFLKSITENVSDEHHRTTLMQKINKL

Target ORF information:

RefSeq Version	XM_004911680.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911680.4

ORF Insert Sequence:

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT CTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCCGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin4 ( XM_004911679.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911736.1 Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa52402
Clone ID Related Accession (Same CDS sequence)	XM_002934341.5 , XM_004911680.4 , XM_004911679.4 , XM_012957516.3
Accession Version	XM_004911679.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911679.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT CTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCCGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911736.1
CDS	473..3679
Translation	MDFGSWNVCEQTAVLQGAFLLAEKLCHPKTLLEVKKSDWPLIQKPITEAFKEISSGLPPQHQEWRRRAIAILWARTLCCTPDLTSVSHFDIDRKWKEDVFFPVENMIPKISQTVLFELLKTMKAADICAELLLALPVGIWAEGATSLLQHICEETSVEDVDFFLDVWWEVMKSKSDEQNETLVLFSSVVSQYLPKSSDEPSQSAKRFKSDPEQTLNQADNILAVFLEGLHKMKQCVATNKLRCFTIANLAEMLCSSAFLEKESELLPIKSYLEQFAVVLSFCEKGSRDVDMIRVAERMVQSGSTSSRFRLLRGAQHFGLSLLKDLLFQWGEELIHYLNNNKVVGYVCYRMRHSLASLHKILRTLEDSGTYPEEQRGDVLELAHCITSFQEKTTQVQHGCPSNANEMMAFIAMKIIENKMDRFKEVCSVFASEVSWAFSNDWLNCLEKNKAHFQDPAMVLKLLDLIVTVTALQSNVDIGSVRKGADVTLSIFSELSLSEKNEVLKEVLSLWGRKGLSPSIKAFTDSFQDELNITFNQISQSLSDYSAAQAVSKVSKLALLNPQAVVKKACHFAVVNVGAHTFLAKILSSLPALCFKCPNNSEKTVSLLCECLMETCWGKLSSDKEERQFLALLAFLMESSEVNDKDSSIQLLQPAEVIQTFVLPYIFDEYANVALCLHILHKAFNVQNPEDESGKHWVLSCSPFPLIMSLCKLLNSYTKCWQLSENSHCLTLGSKELIIDILTLIINAVMPEAGNNSETWNKSLFWLHKKMENLDWVVCLRLKPIFGKHFKNEVPSSLFDVGKLSEREWVPLQLPEYGPGTGLMAWLECCCISAEMKNQMLSLLSVNSKDPEEVNLFSKGFLVAMIQVLPWCSSTECKLVAEVVRHLLKRRLLHVPYTLEYVQHMPLLNLRPFAYNLQFSVLLLRGFQLLCSSSCSDWLPFEGHKHIARLYTNCIFDILEAVKQQISGNKNETQTERKEQDYVQEASFFYMQVFCHVLHIIAMMPDNTCEPLYVLSLEILSLYETLRASDTSTDSFLRRANERHFLKSITENVSDEHHRTTLMQKINKL

Target ORF information:

RefSeq Version	XM_004911679.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911679.4

ORF Insert Sequence:

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT CTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCCGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin4 ( XM_012957516.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812970.1 Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa52402
Clone ID Related Accession (Same CDS sequence)	XM_002934341.5 , XM_004911680.4 , XM_004911679.4 , XM_012957516.3
Accession Version	XM_012957516.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957516.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT CTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCCGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812970.1
CDS	180..3386
Translation	MDFGSWNVCEQTAVLQGAFLLAEKLCHPKTLLEVKKSDWPLIQKPITEAFKEISSGLPPQHQEWRRRAIAILWARTLCCTPDLTSVSHFDIDRKWKEDVFFPVENMIPKISQTVLFELLKTMKAADICAELLLALPVGIWAEGATSLLQHICEETSVEDVDFFLDVWWEVMKSKSDEQNETLVLFSSVVSQYLPKSSDEPSQSAKRFKSDPEQTLNQADNILAVFLEGLHKMKQCVATNKLRCFTIANLAEMLCSSAFLEKESELLPIKSYLEQFAVVLSFCEKGSRDVDMIRVAERMVQSGSTSSRFRLLRGAQHFGLSLLKDLLFQWGEELIHYLNNNKVVGYVCYRMRHSLASLHKILRTLEDSGTYPEEQRGDVLELAHCITSFQEKTTQVQHGCPSNANEMMAFIAMKIIENKMDRFKEVCSVFASEVSWAFSNDWLNCLEKNKAHFQDPAMVLKLLDLIVTVTALQSNVDIGSVRKGADVTLSIFSELSLSEKNEVLKEVLSLWGRKGLSPSIKAFTDSFQDELNITFNQISQSLSDYSAAQAVSKVSKLALLNPQAVVKKACHFAVVNVGAHTFLAKILSSLPALCFKCPNNSEKTVSLLCECLMETCWGKLSSDKEERQFLALLAFLMESSEVNDKDSSIQLLQPAEVIQTFVLPYIFDEYANVALCLHILHKAFNVQNPEDESGKHWVLSCSPFPLIMSLCKLLNSYTKCWQLSENSHCLTLGSKELIIDILTLIINAVMPEAGNNSETWNKSLFWLHKKMENLDWVVCLRLKPIFGKHFKNEVPSSLFDVGKLSEREWVPLQLPEYGPGTGLMAWLECCCISAEMKNQMLSLLSVNSKDPEEVNLFSKGFLVAMIQVLPWCSSTECKLVAEVVRHLLKRRLLHVPYTLEYVQHMPLLNLRPFAYNLQFSVLLLRGFQLLCSSSCSDWLPFEGHKHIARLYTNCIFDILEAVKQQISGNKNETQTERKEQDYVQEASFFYMQVFCHVLHIIAMMPDNTCEPLYVLSLEILSLYETLRASDTSTDSFLRRANERHFLKSITENVSDEHHRTTLMQKINKL

Target ORF information:

RefSeq Version	XM_012957516.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957516.3

ORF Insert Sequence:

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT CTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCCGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin4 ( XM_004911680.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911737.1 Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X2, mRNA.

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CloneID	OXa05636
Clone ID Related Accession (Same CDS sequence)	XM_004911680.3 , XM_002934341.4 , XM_012957516.2 , XM_004911679.3
Accession Version	XM_004911680.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911680.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT TTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCTGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911737.1
CDS	431..3637
Translation	MDFGSWNVCEQTAVLQGAFLLAEKLCHPKTLLEVKKSDWPLIQKPITEAFKEISSGLPPQHQEWRRRAIAILWARTLCCTPDLTSVSHFDIDRKWKEDVFFPVENMIPKISQTVLFELLKTMKAADICAELLLALPVGIWAEGATSLLQHICEETSVEDVDFFLDVWWEVMKSKSDEQNETLVLFSSVVSQYLPKSSDEPSQSAKRFKSDPEQTLNQADNILAVFLEGLHKMKQCVATNKLRCFTIANLAEMLCSSAFLEKESELLPIKSYLEQFAVVLSFCEKGSRDVDMIRVAERMVQSGSTSSRFRLLRGAQHFGLSLLKDLLFQWGEELIHYLNNNKVVGYVCYRMRHSLASLHKILRTLEDSGTYPEEQRGDVLELAHCITSFQEKTTQVQHGCPSNANEMMAFIAMKIIENKMDRFKEVCSVFASEVSWAFSNDWLNCLEKNKAHFQDPAMVLKLLDLIVTVTALQSNVDIGSVRKGADVTLSIFSELSLSEKNEVLKEVLSLWGRKGLSPSIKAFTDSFQDELNITFNQISQSLSDYSAAQAVSKVSKLALLNPQAVVKKACHFAVVNVGAHTFLAKILSSLPALCFKCPNNSEKTVSLLCECLMETCWGKLSSDKEERQFLALLAFLMESSEVNDKDSSIQLLQPAEVIQTFVLPYIFDEYANVALCLHILHKAFNVQNPEDESGKHWVLSCSPFPLIMSLCKLLNSYTKCWQLSENSHCLTLGSKELIIDILTLIINAVMPEAGNNSETWNKSLFWLHKKMENLDWVVCLRLKPIFGKHFKNEVPSSLFDVGKLSEREWVPLQLPEYGPGTGLMAWLECCCISAEMKNQMLSLLSVNSKDPEEVNLFSKGFLVAMIQVLPWCSSTECKLVAEVVRHLLKRRLLHVPYTLEYVQHMPLLNLRPFAYNLQFSVLLLRGFQLLCSSSCSDWLPFEGHKHIARLYTNCIFDILEAVKQQISGNKNETQTERKEQDYVQEASFFYMQVFCHVLHIIAMMPDNTCEPLYVLSLEILSLYETLRASDTSTDSFLRRANERHFLKSITENVSDEHHRTTLMQKINKL

Target ORF information:

RefSeq Version	XM_004911680.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911680.3

ORF Insert Sequence:

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT TTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCTGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin4 ( XM_002934341.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934387.1 Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa05636
Clone ID Related Accession (Same CDS sequence)	XM_004911680.3 , XM_002934341.4 , XM_012957516.2 , XM_004911679.3
Accession Version	XM_002934341.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934341.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT TTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCTGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934387.1
CDS	235..3441
Translation	MDFGSWNVCEQTAVLQGAFLLAEKLCHPKTLLEVKKSDWPLIQKPITEAFKEISSGLPPQHQEWRRRAIAILWARTLCCTPDLTSVSHFDIDRKWKEDVFFPVENMIPKISQTVLFELLKTMKAADICAELLLALPVGIWAEGATSLLQHICEETSVEDVDFFLDVWWEVMKSKSDEQNETLVLFSSVVSQYLPKSSDEPSQSAKRFKSDPEQTLNQADNILAVFLEGLHKMKQCVATNKLRCFTIANLAEMLCSSAFLEKESELLPIKSYLEQFAVVLSFCEKGSRDVDMIRVAERMVQSGSTSSRFRLLRGAQHFGLSLLKDLLFQWGEELIHYLNNNKVVGYVCYRMRHSLASLHKILRTLEDSGTYPEEQRGDVLELAHCITSFQEKTTQVQHGCPSNANEMMAFIAMKIIENKMDRFKEVCSVFASEVSWAFSNDWLNCLEKNKAHFQDPAMVLKLLDLIVTVTALQSNVDIGSVRKGADVTLSIFSELSLSEKNEVLKEVLSLWGRKGLSPSIKAFTDSFQDELNITFNQISQSLSDYSAAQAVSKVSKLALLNPQAVVKKACHFAVVNVGAHTFLAKILSSLPALCFKCPNNSEKTVSLLCECLMETCWGKLSSDKEERQFLALLAFLMESSEVNDKDSSIQLLQPAEVIQTFVLPYIFDEYANVALCLHILHKAFNVQNPEDESGKHWVLSCSPFPLIMSLCKLLNSYTKCWQLSENSHCLTLGSKELIIDILTLIINAVMPEAGNNSETWNKSLFWLHKKMENLDWVVCLRLKPIFGKHFKNEVPSSLFDVGKLSEREWVPLQLPEYGPGTGLMAWLECCCISAEMKNQMLSLLSVNSKDPEEVNLFSKGFLVAMIQVLPWCSSTECKLVAEVVRHLLKRRLLHVPYTLEYVQHMPLLNLRPFAYNLQFSVLLLRGFQLLCSSSCSDWLPFEGHKHIARLYTNCIFDILEAVKQQISGNKNETQTERKEQDYVQEASFFYMQVFCHVLHIIAMMPDNTCEPLYVLSLEILSLYETLRASDTSTDSFLRRANERHFLKSITENVSDEHHRTTLMQKINKL

Target ORF information:

RefSeq Version	XM_002934341.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934341.4

ORF Insert Sequence:

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT TTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCTGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin4 ( XM_012957516.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812970.1 Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa05636
Clone ID Related Accession (Same CDS sequence)	XM_004911680.3 , XM_002934341.4 , XM_012957516.2 , XM_004911679.3
Accession Version	XM_012957516.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957516.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT TTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCTGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812970.1
CDS	380..3586
Translation	MDFGSWNVCEQTAVLQGAFLLAEKLCHPKTLLEVKKSDWPLIQKPITEAFKEISSGLPPQHQEWRRRAIAILWARTLCCTPDLTSVSHFDIDRKWKEDVFFPVENMIPKISQTVLFELLKTMKAADICAELLLALPVGIWAEGATSLLQHICEETSVEDVDFFLDVWWEVMKSKSDEQNETLVLFSSVVSQYLPKSSDEPSQSAKRFKSDPEQTLNQADNILAVFLEGLHKMKQCVATNKLRCFTIANLAEMLCSSAFLEKESELLPIKSYLEQFAVVLSFCEKGSRDVDMIRVAERMVQSGSTSSRFRLLRGAQHFGLSLLKDLLFQWGEELIHYLNNNKVVGYVCYRMRHSLASLHKILRTLEDSGTYPEEQRGDVLELAHCITSFQEKTTQVQHGCPSNANEMMAFIAMKIIENKMDRFKEVCSVFASEVSWAFSNDWLNCLEKNKAHFQDPAMVLKLLDLIVTVTALQSNVDIGSVRKGADVTLSIFSELSLSEKNEVLKEVLSLWGRKGLSPSIKAFTDSFQDELNITFNQISQSLSDYSAAQAVSKVSKLALLNPQAVVKKACHFAVVNVGAHTFLAKILSSLPALCFKCPNNSEKTVSLLCECLMETCWGKLSSDKEERQFLALLAFLMESSEVNDKDSSIQLLQPAEVIQTFVLPYIFDEYANVALCLHILHKAFNVQNPEDESGKHWVLSCSPFPLIMSLCKLLNSYTKCWQLSENSHCLTLGSKELIIDILTLIINAVMPEAGNNSETWNKSLFWLHKKMENLDWVVCLRLKPIFGKHFKNEVPSSLFDVGKLSEREWVPLQLPEYGPGTGLMAWLECCCISAEMKNQMLSLLSVNSKDPEEVNLFSKGFLVAMIQVLPWCSSTECKLVAEVVRHLLKRRLLHVPYTLEYVQHMPLLNLRPFAYNLQFSVLLLRGFQLLCSSSCSDWLPFEGHKHIARLYTNCIFDILEAVKQQISGNKNETQTERKEQDYVQEASFFYMQVFCHVLHIIAMMPDNTCEPLYVLSLEILSLYETLRASDTSTDSFLRRANERHFLKSITENVSDEHHRTTLMQKINKL

Target ORF information:

RefSeq Version	XM_012957516.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957516.2

ORF Insert Sequence:

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT TTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCTGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin4 ( XM_004911679.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911736.1 Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X1, mRNA.

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CloneID	OXa05636
Clone ID Related Accession (Same CDS sequence)	XM_004911680.3 , XM_002934341.4 , XM_012957516.2 , XM_004911679.3
Accession Version	XM_004911679.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gem-associated protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911679.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT TTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCTGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911736.1
CDS	864..4070
Translation	MDFGSWNVCEQTAVLQGAFLLAEKLCHPKTLLEVKKSDWPLIQKPITEAFKEISSGLPPQHQEWRRRAIAILWARTLCCTPDLTSVSHFDIDRKWKEDVFFPVENMIPKISQTVLFELLKTMKAADICAELLLALPVGIWAEGATSLLQHICEETSVEDVDFFLDVWWEVMKSKSDEQNETLVLFSSVVSQYLPKSSDEPSQSAKRFKSDPEQTLNQADNILAVFLEGLHKMKQCVATNKLRCFTIANLAEMLCSSAFLEKESELLPIKSYLEQFAVVLSFCEKGSRDVDMIRVAERMVQSGSTSSRFRLLRGAQHFGLSLLKDLLFQWGEELIHYLNNNKVVGYVCYRMRHSLASLHKILRTLEDSGTYPEEQRGDVLELAHCITSFQEKTTQVQHGCPSNANEMMAFIAMKIIENKMDRFKEVCSVFASEVSWAFSNDWLNCLEKNKAHFQDPAMVLKLLDLIVTVTALQSNVDIGSVRKGADVTLSIFSELSLSEKNEVLKEVLSLWGRKGLSPSIKAFTDSFQDELNITFNQISQSLSDYSAAQAVSKVSKLALLNPQAVVKKACHFAVVNVGAHTFLAKILSSLPALCFKCPNNSEKTVSLLCECLMETCWGKLSSDKEERQFLALLAFLMESSEVNDKDSSIQLLQPAEVIQTFVLPYIFDEYANVALCLHILHKAFNVQNPEDESGKHWVLSCSPFPLIMSLCKLLNSYTKCWQLSENSHCLTLGSKELIIDILTLIINAVMPEAGNNSETWNKSLFWLHKKMENLDWVVCLRLKPIFGKHFKNEVPSSLFDVGKLSEREWVPLQLPEYGPGTGLMAWLECCCISAEMKNQMLSLLSVNSKDPEEVNLFSKGFLVAMIQVLPWCSSTECKLVAEVVRHLLKRRLLHVPYTLEYVQHMPLLNLRPFAYNLQFSVLLLRGFQLLCSSSCSDWLPFEGHKHIARLYTNCIFDILEAVKQQISGNKNETQTERKEQDYVQEASFFYMQVFCHVLHIIAMMPDNTCEPLYVLSLEILSLYETLRASDTSTDSFLRRANERHFLKSITENVSDEHHRTTLMQKINKL

Target ORF information:

RefSeq Version	XM_004911679.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gem nuclear organelle associated protein 4 (gemin4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911679.3

ORF Insert Sequence:

ATGGATTTTG GAAGCTGGAA TGTGTGTGAG CAAACTGCGG TTCTACAGGG TGCATTTTTA 
TTGGCTGAAA AACTTTGCCA TCCAAAAACA TTGCTGGAGG TAAAGAAGTC TGACTGGCCC 
CTTATTCAAA AACCAATCAC AGAGGCCTTC AAGGAAATAA GCTCAGGTCT TCCACCTCAA 
CATCAAGAAT GGCGGAGAAG AGCCATTGCT ATACTTTGGG CCAGAACACT TTGTTGCACG 
CCAGATCTGA CTTCCGTTTC ACACTTTGAC ATAGATAGGA AATGGAAAGA GGATGTTTTC 
TTTCCAGTGG AAAACATGAT CCCCAAAATA AGTCAAACTG TACTCTTTGA ACTGCTCAAA 
ACAATGAAAG CAGCAGACAT TTGTGCAGAA TTGCTGTTGG CACTTCCAGT TGGCATTTGG 
GCTGAAGGGG CCACCTCACT ACTTCAGCAT ATTTGTGAAG AGACTTCAGT AGAGGATGTT 
GATTTTTTTT TAGATGTCTG GTGGGAGGTA ATGAAAAGCA AGAGTGATGA GCAAAATGAA 
ACTCTTGTGT TATTTTCCTC TGTGGTTTCC CAGTACTTAC CAAAGTCTAG TGATGAACCA 
TCACAGAGCG CCAAAAGATT TAAGTCAGAT CCTGAACAGA CTTTAAATCA AGCCGATAAT 
ATCCTTGCTG TTTTTCTAGA GGGGCTTCAT AAAATGAAAC AGTGTGTTGC TACCAACAAG 
CTGAGGTGCT TTACTATTGC AAATCTAGCT GAAATGTTAT GCTCCTCAGC CTTTCTCGAA 
AAGGAGTCTG AGCTTCTGCC CATAAAATCA TATTTAGAAC AATTTGCAGT AGTGCTTTCC 
TTCTGTGAAA AAGGATCCAG AGATGTTGAT ATGATTAGAG TAGCAGAAAG GATGGTGCAG 
AGCGGAAGCA CATCTTCAAG ATTCCGCTTA CTACGAGGTG CTCAGCATTT TGGTTTAAGC 
CTTCTCAAAG ACCTTCTTTT TCAGTGGGGG GAGGAGTTAA TTCATTATTT GAATAACAAC 
AAAGTAGTTG GCTATGTATG TTACAGGATG AGACACAGCC TTGCATCTTT GCATAAAATC 
TTGAGAACGT TAGAAGATTC TGGTACCTAC CCTGAAGAAC AAAGAGGGGA TGTACTGGAA 
TTGGCACACT GCATTACAAG CTTTCAAGAG AAAACCACCC AAGTTCAACA TGGATGTCCT 
TCTAATGCTA ATGAAATGAT GGCTTTTATT GCCATGAAAA TTATTGAGAA TAAAATGGAC 
CGTTTCAAAG AAGTTTGTTC TGTCTTCGCC TCAGAAGTAT CCTGGGCCTT TTCAAATGAC 
TGGTTAAATT GTCTTGAGAA AAACAAAGCA CATTTTCAGG ACCCTGCAAT GGTATTAAAA 
TTGCTGGATC TCATAGTAAC AGTTACTGCT TTACAAAGTA ATGTGGATAT TGGATCAGTA 
CGAAAAGGCG CTGATGTCAC ATTAAGCATT TTTTCAGAAC TTTCACTGTC TGAGAAGAAT 
GAAGTGCTGA AAGAAGTTTT ATCCTTATGG GGCAGAAAAG GGCTTTCTCC ATCTATCAAA 
GCATTTACTG ACAGTTTCCA AGATGAACTT AATATAACAT TCAACCAAAT TTCCCAAAGC 
CTTTCTGATT ACAGTGCTGC TCAGGCTGTT TCAAAGGTGT CTAAACTGGC TTTGCTGAAC 
CCGCAAGCAG TAGTAAAGAA AGCCTGTCAT TTTGCAGTGG TAAATGTTGG TGCTCATACA 
TTTTTAGCCA AGATATTGAG CTCCCTTCCA GCTTTATGCT TTAAGTGTCC TAATAATTCA 
GAAAAAACAG TCTCTCTGTT GTGTGAGTGC CTGATGGAAA CCTGTTGGGG TAAGTTGTCT 
TCTGATAAGG AAGAGAGGCA GTTTTTAGCC TTACTAGCCT TTCTGATGGA ATCTTCAGAA 
GTAAATGATA AGGATTCTTC TATCCAACTT CTCCAGCCAG CTGAAGTCAT ACAAACATTT 
GTTCTCCCGT ACATTTTTGA CGAATATGCT AATGTTGCGT TATGCTTGCA CATACTTCAT 
AAGGCTTTTA ATGTTCAGAA TCCTGAGGAT GAATCTGGAA AACATTGGGT TTTATCCTGT 
TCCCCGTTTC CACTTATTAT GTCATTATGT AAGCTTCTTA ATTCCTACAC AAAATGCTGG 
CAACTTTCTG AGAATTCACA TTGCCTTACA CTGGGCTCCA AAGAGTTAAT CATTGACATT 
CTTACACTGA TCATTAATGC AGTCATGCCC GAGGCTGGAA ACAACTCTGA AACGTGGAAC 
AAGTCACTCT TCTGGTTGCA TAAAAAAATG GAAAACTTGG ATTGGGTTGT ATGTTTGAGG 
CTAAAACCAA TATTTGGAAA ACACTTTAAA AACGAAGTCC CATCTTCATT ATTTGACGTT 
GGCAAACTGT CTGAGAGGGA ATGGGTTCCT CTTCAGCTAC CCGAGTATGG ACCAGGCACA 
GGTCTTATGG CATGGCTGGA ATGTTGCTGC ATATCTGCAG AAATGAAAAA CCAAATGCTT 
TCTCTTCTCT CTGTCAACAG CAAAGACCCT GAGGAGGTCA ACCTTTTTAG TAAAGGTTTT 
CTGGTGGCAA TGATCCAAGT TCTACCATGG TGTAGCTCAA CAGAATGCAA GCTTGTTGCT 
GAGGTAGTGA GACATCTCCT TAAAAGACGA CTCCTTCATG TACCCTACAC TCTAGAATAT 
GTACAGCATA TGCCACTTTT GAATCTGCGA CCATTTGCTT ATAATCTCCA GTTCTCTGTT 
CTTCTTCTCA GAGGCTTTCA GCTTCTATGT AGTTCTAGTT GTTCTGATTG GCTACCTTTT 
GAAGGGCACA AACACATTGC AAGGCTTTAT ACTAACTGCA TATTTGATAT ATTAGAGGCT 
GTCAAGCAGC AGATATCAGG AAATAAAAAT GAGACCCAGA CAGAAAGGAA AGAACAGGAC 
TATGTTCAGG AGGCTTCCTT CTTTTATATG CAGGTATTCT GCCATGTTCT TCATATTATA 
GCAATGATGC CGGACAATAC ATGTGAGCCG CTTTATGTAC TTTCACTTGA GATTCTGTCC 
CTGTATGAGA CTCTAAGGGC AAGTGACACT TCAACAGACA GTTTTCTCAG AAGAGCTAAC 
GAGAGGCATT TCTTGAAATC CATTACTGAG AATGTAAGTG ATGAGCACCA TCGCACAACT 
CTAATGCAAA AAATTAATAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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gemin4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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