GEMIN4 cDNA ORF clone, Bos taurus(cattle)

The following GEMIN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GEMIN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa10037 XM_024980697.1
Latest version!
Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa10037 XM_010815869.3
Latest version!
Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa10038 XM_585199.8
Latest version!
Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa10038 XM_002695679.5
Latest version!
Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa29565 XM_010815869.2
Latest version!
Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa29565 XM_010824016.2
Latest version!
Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa54209 XM_010815870.2
Latest version!
Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa54209 XM_010824017.2
Latest version!
Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa10038 XM_002695679.4 Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa10037
    Clone ID Related Accession (Same CDS sequence) XM_024980697.1 , XM_010815869.3
    Accession Version XM_024980697.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product gem-associated protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGAAGTG GTGTGTCCAT 
    GCAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT
    GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT
    GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC
    TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC
    ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC
    ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT
    GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG
    CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC
    GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC
    CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG
    TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG
    CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC
    TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG
    ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC
    CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG
    ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC
    CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC
    TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC
    CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC
    GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC
    ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG
    TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG
    CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG
    ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC
    TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG
    GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC
    TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC
    TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG
    AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT
    CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA
    TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG
    AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA
    GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG
    CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC
    ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG
    TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC
    CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA
    GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG
    AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC
    TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG
    ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC
    TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC
    AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG
    CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG
    AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC
    CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT
    CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG
    CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC
    CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG
    GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC
    TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC
    GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC
    ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836465.1
    CDS259..3492
    Translation

    Target ORF information:

    RefSeq Version XM_024980697.1
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980697.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGAAGTG GTGTGTCCAT 
    GCAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT
    GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT
    GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC
    TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC
    ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC
    ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT
    GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG
    CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC
    GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC
    CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG
    TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG
    CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC
    TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG
    ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC
    CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG
    ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC
    CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC
    TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC
    CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC
    GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC
    ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG
    TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG
    CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG
    ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC
    TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG
    GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC
    TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC
    TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG
    AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT
    CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA
    TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG
    AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA
    GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG
    CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC
    ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG
    TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC
    CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA
    GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG
    AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC
    TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG
    ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC
    TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC
    AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG
    CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG
    AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC
    CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT
    CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG
    CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC
    CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG
    GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC
    TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC
    GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC
    ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10037
    Clone ID Related Accession (Same CDS sequence) XM_024980697.1 , XM_010815869.3
    Accession Version XM_010815869.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product gem-associated protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815869.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGAAGTG GTGTGTCCAT 
    GCAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT
    GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT
    GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC
    TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC
    ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC
    ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT
    GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG
    CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC
    GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC
    CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG
    TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG
    CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC
    TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG
    ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC
    CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG
    ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC
    CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC
    TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC
    CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC
    GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC
    ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG
    TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG
    CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG
    ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC
    TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG
    GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC
    TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC
    TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG
    AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT
    CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA
    TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG
    AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA
    GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG
    CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC
    ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG
    TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC
    CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA
    GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG
    AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC
    TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG
    ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC
    TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC
    AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG
    CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG
    AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC
    CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT
    CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG
    CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC
    CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG
    GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC
    TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC
    GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC
    ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814171.2
    CDS276..3509
    Translation

    Target ORF information:

    RefSeq Version XM_010815869.3
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815869.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGAAGTG GTGTGTCCAT 
    GCAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT
    GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT
    GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC
    TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC
    ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC
    ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT
    GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG
    CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC
    GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC
    CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG
    TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG
    CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC
    TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG
    ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC
    CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG
    ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC
    CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC
    TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC
    CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC
    GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC
    ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG
    TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG
    CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG
    ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC
    TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG
    GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC
    TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC
    TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG
    AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT
    CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA
    TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG
    AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA
    GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG
    CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC
    ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG
    TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC
    CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA
    GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG
    AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC
    TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG
    ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC
    TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC
    AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG
    CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG
    AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC
    CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT
    CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG
    CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC
    CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG
    GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC
    TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC
    GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC
    ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10038
    Clone ID Related Accession (Same CDS sequence) XM_585199.8 , XM_002695679.4 , XM_002695679.5
    Accession Version XM_585199.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product gem-associated protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_585199.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACCTAG GACCTTTGAA TATCTGTGAG GAAATGACTA TCTTGCACGG GGGCTTCTTG 
    CTGGCTGAGC AGCTGTTCCG CCCCAAAGCG CTGGCCGAAC TGACCAAGTC TGACTGGGTG
    CACGTTGGGC GGCCCATTGT GGAGGCTCTG AGGGAGATCT CCTCCACCAC AGCCTGCTCC
    CAGCCCTTCG CCTGGAAGAA GAAAGCGCTG ATCATCATCT GGGCCAAGGT GCTACAGCCC
    TACCCCATCA CCCCTTCCGA CATGGAAACC CGCTGGCAGG AAGATGTGTT CTTCTCTGTG
    GGCAACATGA TCCCCACCAT CAATCACACA GTCCTCTTTG AGCTGCTCAA GTCGCTGGAG
    GCCTCTGGAC TCTTTATCCA GCTCCTGATG GCCCTGCCCA CCACTGTCTG CCGTGCGGAA
    CTAGAGCACT TTTTGGAGCA CATGACCATC GACACCTCTT CCAAGGACGT GGCCTTCTTC
    CTCGACGTCT GGTGGGAAAT GATGAAGCAC AAGGGCAATC AGCAGGATCC CCTGCTCTCC
    CAGTTCCGGA CAATGGCCCA CAAGTACCTG TCCTCCTCCG ATGACTTCTC CCACCCGCCG
    AAGAGGTTCA AGTCCGATCC AGATGTGTGT CCAACCATGC CCCTGCTGGC CATGCTGCTC
    ACGGGGCTGA AACAAATCCA GGACAGGATC CTATGCCCCG GGATGAAGTG CTGCGCCTTA
    GCCAACTTGG CTGACATGCT GACCGTGTTT GCGCTGATGG AGGAGGACCC CCAGGAAGTG
    TCTGCGACCG TGTACCTGGA CAAGCTGGCC ACGGTGATCT CCGTGTGGAA CTCGGACACC
    CAGAACCCGT ATCACCAGCA GGCGCTGGCA GAGAAGGTGA AGGAGGCGGA GCGGGACATC
    AGCCTGACCT CCCTGGCCAG GCTGCCGAGC GAGACCATCT TCGTGGGCTT TGAGTTCCTG
    CGCAGCCTGC TGCAGGAGTG GGGGGAGGAG CTGCAGGCCT TGCTCAGCAG CAGCCAGGGG
    ACCAGCTACG ACAGCTTCCG GCTCTGCGAC AGCCTGACCT CCTTCAGCCA GAATCTGAAG
    CTCTACCTGG ACACCACCAG CCTGGCCAAG GAGGAGAGGC AGGTGGTCTC CGAGCTGGCA
    GAGTGCGTGG GGGACTTCCT GAGGAAGACG AACAGGGTGG TGAGGAGCAA GGGCGGTGAG
    CAGGACATCA CCGCCTCCAT CGCCATGGCC ATCATCGAGC AGAAGATGGA CCGCCACATG
    GAAATGTGCT ACATTTTTGC CTCCGAGAGG AAGTGGGCCT TCTCGGACGA GTGGCTGTCC
    TGCCTGCTCA ACAACCGAGC TCTCTTCCGA GAGCCGGGCC TGGTGTTAAA GCTGCTGGAG
    ACGGTGATGG AAGTCAGCAC GATAGACAGG GCTGTCTCCG AACCTCAGAT CAAACAGGTG
    TTCGACTTGA TCCTGGAGTG TTACACACAC CTCTCTCTGC CAGATAAAAA TAAAGTCCTC
    TCGGGGGTCC TGCTCTCCTG GGGGCGCAAG GGCCTCTCTG AGAAATTGTT AGCTCACTTG
    GAGGGCTTTC AGGAGGACCT CAACACGACT TTCAACCAGC TCACACAGAG CACCTCTGAA
    CAGGGCTTGG CTAAGGCTGT TGCTTCCGTG GCCCGCCTGG TCATCCTGCA CCCAGAGATC
    ACGGTGAAGA AGTTGTGCAG CATGGCTGTG GTCAACCTCG GCACCCACAA GTTCCTGGCT
    CAGATTCTCA CTGCCTTCCC CGCTCTCCGG TTCATGGAAG AGCAGGGGCC AGACCGCTCT
    ACCACATTCG TGGTGTCCTG CCTCAAAGAA ACGGTCTGGA CCAAGCTCTC GACACCCCGG
    GAGGAGAAGC AGTTTCTGGA GCTCTTGAGC TGCCTGGTGA GTCCTACAAA GCCCCAAGGG
    ATTCCAGTCG CTGCTCTGCT CGAGCCGGAT GAGGTACTAA AGGAGTTCGT CCTGCCTTTC
    TTGATGCTGG AGGTCAAAGA GGTGGACCTC AGTCTGAGGA TCTTCGTCCA GACTCTGGAA
    GGGAGCACAA ACCTGGAAGA ATACTGGCTG CAGTCCTGCT CTCCGTTCCC GCTCATCTTC
    AGCCTGTGCC AGCTCCTTGA TGGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAGCGC
    TGTCTCCCCC TGGACGGGAA GGACCTGGCC ATCCACATCC TGGAGCTCCT CTGTGAGGTG
    GTCTCAGCTA ACACCGACAC CTTCTCCCCA GACACCTGGG TCAAGTCGCT GTCCTGGCTC
    CACCGGAAGC TGGAGCAGCT AGACTGGACT GTGGGCCTGA GACTGAAGAG CTTCTTTGAG
    GGCCACTTCA AGTGCGAGGT GCCAGCCACG CTCTTTGAGA TCTGTAAGCT TTCTGAGGAC
    GAGTGGACCT CCCAGGCCCA CCCCGGGTAT GGGCCTGGCA CAGGCCTCCT GGCCTGGATG
    GAGTGCTCCT CCATCTCCAG CAGCGTCTCC GAGCAGATGC TCGCACTCCT GGTGGTGGAC
    GTGGGCAACC CTGAGGAGGT CAGGTTGTTC AGCAAGGGCT TCCTGGTGGC CCTGGTACAG
    GTCATGCCAT GGTGCAGCCC CCAGGAATGG CAGTGCCTTC ACCAGCTGAC CCGGAGACTG
    TTGGAGAAGC AACTCCTGCA CGTCCCCTAC AGCCTGGAGT ATATACAGTT CGTTCCTCTG
    CTCAACCTGA AGCCCTTTGC CCAGGAGCTC CAGCTCTCTG TCCTCTTGCT TAGAGTTTTT
    CAGTTTCTCT GCAGCCAGAG CTGCCGTAAC TGGCTCCCTG TGGAGGGCTG GAGCCATGTG
    GTCAAGCTCC TCTGCAACAG TCTGACCAAT CTCCTGGACT CTGTTCGGTT GATACAGTCG
    GTTGGCCCTT GGGCTCAAGG ACAAGAGCAG GACCTGACCC AGGAAACCCT CTTTTTCTAT
    ACCCAGGTCT TCTGTCATGT TCTGCACATC ATGGCCATGG TCCACCAGGA GGTCTGTGAA
    CCTCTCTATG TCTTGGCCTT GGAGATCCTC ACCTGCTACG AAACCCTGAG CAAGACCAAC
    CCTTCCGTCA GCTCCTTGCT CCAGAAAGTA AACGAGCAGC GCTTCTTAAA GTCCATCGCC
    GAGAACATCA GCCCTGAGGA GCGGCGCCAA ACCCTCCTGC AGAAGATCAG CAACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_585199.4
    CDS184..3363
    Translation

    Target ORF information:

    RefSeq Version XM_585199.8
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_585199.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACCTAG GACCTTTGAA TATCTGTGAG GAAATGACTA TCTTGCACGG GGGCTTCTTG 
    CTGGCTGAGC AGCTGTTCCG CCCCAAAGCG CTGGCCGAAC TGACCAAGTC TGACTGGGTG
    CACGTTGGGC GGCCCATTGT GGAGGCTCTG AGGGAGATCT CCTCCACCAC AGCCTGCTCC
    CAGCCCTTCG CCTGGAAGAA GAAAGCGCTG ATCATCATCT GGGCCAAGGT GCTACAGCCC
    TACCCCATCA CCCCTTCCGA CATGGAAACC CGCTGGCAGG AAGATGTGTT CTTCTCTGTG
    GGCAACATGA TCCCCACCAT CAATCACACA GTCCTCTTTG AGCTGCTCAA GTCGCTGGAG
    GCCTCTGGAC TCTTTATCCA GCTCCTGATG GCCCTGCCCA CCACTGTCTG CCGTGCGGAA
    CTAGAGCACT TTTTGGAGCA CATGACCATC GACACCTCTT CCAAGGACGT GGCCTTCTTC
    CTCGACGTCT GGTGGGAAAT GATGAAGCAC AAGGGCAATC AGCAGGATCC CCTGCTCTCC
    CAGTTCCGGA CAATGGCCCA CAAGTACCTG TCCTCCTCCG ATGACTTCTC CCACCCGCCG
    AAGAGGTTCA AGTCCGATCC AGATGTGTGT CCAACCATGC CCCTGCTGGC CATGCTGCTC
    ACGGGGCTGA AACAAATCCA GGACAGGATC CTATGCCCCG GGATGAAGTG CTGCGCCTTA
    GCCAACTTGG CTGACATGCT GACCGTGTTT GCGCTGATGG AGGAGGACCC CCAGGAAGTG
    TCTGCGACCG TGTACCTGGA CAAGCTGGCC ACGGTGATCT CCGTGTGGAA CTCGGACACC
    CAGAACCCGT ATCACCAGCA GGCGCTGGCA GAGAAGGTGA AGGAGGCGGA GCGGGACATC
    AGCCTGACCT CCCTGGCCAG GCTGCCGAGC GAGACCATCT TCGTGGGCTT TGAGTTCCTG
    CGCAGCCTGC TGCAGGAGTG GGGGGAGGAG CTGCAGGCCT TGCTCAGCAG CAGCCAGGGG
    ACCAGCTACG ACAGCTTCCG GCTCTGCGAC AGCCTGACCT CCTTCAGCCA GAATCTGAAG
    CTCTACCTGG ACACCACCAG CCTGGCCAAG GAGGAGAGGC AGGTGGTCTC CGAGCTGGCA
    GAGTGCGTGG GGGACTTCCT GAGGAAGACG AACAGGGTGG TGAGGAGCAA GGGCGGTGAG
    CAGGACATCA CCGCCTCCAT CGCCATGGCC ATCATCGAGC AGAAGATGGA CCGCCACATG
    GAAATGTGCT ACATTTTTGC CTCCGAGAGG AAGTGGGCCT TCTCGGACGA GTGGCTGTCC
    TGCCTGCTCA ACAACCGAGC TCTCTTCCGA GAGCCGGGCC TGGTGTTAAA GCTGCTGGAG
    ACGGTGATGG AAGTCAGCAC GATAGACAGG GCTGTCTCCG AACCTCAGAT CAAACAGGTG
    TTCGACTTGA TCCTGGAGTG TTACACACAC CTCTCTCTGC CAGATAAAAA TAAAGTCCTC
    TCGGGGGTCC TGCTCTCCTG GGGGCGCAAG GGCCTCTCTG AGAAATTGTT AGCTCACTTG
    GAGGGCTTTC AGGAGGACCT CAACACGACT TTCAACCAGC TCACACAGAG CACCTCTGAA
    CAGGGCTTGG CTAAGGCTGT TGCTTCCGTG GCCCGCCTGG TCATCCTGCA CCCAGAGATC
    ACGGTGAAGA AGTTGTGCAG CATGGCTGTG GTCAACCTCG GCACCCACAA GTTCCTGGCT
    CAGATTCTCA CTGCCTTCCC CGCTCTCCGG TTCATGGAAG AGCAGGGGCC AGACCGCTCT
    ACCACATTCG TGGTGTCCTG CCTCAAAGAA ACGGTCTGGA CCAAGCTCTC GACACCCCGG
    GAGGAGAAGC AGTTTCTGGA GCTCTTGAGC TGCCTGGTGA GTCCTACAAA GCCCCAAGGG
    ATTCCAGTCG CTGCTCTGCT CGAGCCGGAT GAGGTACTAA AGGAGTTCGT CCTGCCTTTC
    TTGATGCTGG AGGTCAAAGA GGTGGACCTC AGTCTGAGGA TCTTCGTCCA GACTCTGGAA
    GGGAGCACAA ACCTGGAAGA ATACTGGCTG CAGTCCTGCT CTCCGTTCCC GCTCATCTTC
    AGCCTGTGCC AGCTCCTTGA TGGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAGCGC
    TGTCTCCCCC TGGACGGGAA GGACCTGGCC ATCCACATCC TGGAGCTCCT CTGTGAGGTG
    GTCTCAGCTA ACACCGACAC CTTCTCCCCA GACACCTGGG TCAAGTCGCT GTCCTGGCTC
    CACCGGAAGC TGGAGCAGCT AGACTGGACT GTGGGCCTGA GACTGAAGAG CTTCTTTGAG
    GGCCACTTCA AGTGCGAGGT GCCAGCCACG CTCTTTGAGA TCTGTAAGCT TTCTGAGGAC
    GAGTGGACCT CCCAGGCCCA CCCCGGGTAT GGGCCTGGCA CAGGCCTCCT GGCCTGGATG
    GAGTGCTCCT CCATCTCCAG CAGCGTCTCC GAGCAGATGC TCGCACTCCT GGTGGTGGAC
    GTGGGCAACC CTGAGGAGGT CAGGTTGTTC AGCAAGGGCT TCCTGGTGGC CCTGGTACAG
    GTCATGCCAT GGTGCAGCCC CCAGGAATGG CAGTGCCTTC ACCAGCTGAC CCGGAGACTG
    TTGGAGAAGC AACTCCTGCA CGTCCCCTAC AGCCTGGAGT ATATACAGTT CGTTCCTCTG
    CTCAACCTGA AGCCCTTTGC CCAGGAGCTC CAGCTCTCTG TCCTCTTGCT TAGAGTTTTT
    CAGTTTCTCT GCAGCCAGAG CTGCCGTAAC TGGCTCCCTG TGGAGGGCTG GAGCCATGTG
    GTCAAGCTCC TCTGCAACAG TCTGACCAAT CTCCTGGACT CTGTTCGGTT GATACAGTCG
    GTTGGCCCTT GGGCTCAAGG ACAAGAGCAG GACCTGACCC AGGAAACCCT CTTTTTCTAT
    ACCCAGGTCT TCTGTCATGT TCTGCACATC ATGGCCATGG TCCACCAGGA GGTCTGTGAA
    CCTCTCTATG TCTTGGCCTT GGAGATCCTC ACCTGCTACG AAACCCTGAG CAAGACCAAC
    CCTTCCGTCA GCTCCTTGCT CCAGAAAGTA AACGAGCAGC GCTTCTTAAA GTCCATCGCC
    GAGAACATCA GCCCTGAGGA GCGGCGCCAA ACCCTCCTGC AGAAGATCAG CAACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10038
    Clone ID Related Accession (Same CDS sequence) XM_585199.8 , XM_002695679.4 , XM_002695679.5
    Accession Version XM_002695679.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product gem-associated protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002695679.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACCTAG GACCTTTGAA TATCTGTGAG GAAATGACTA TCTTGCACGG GGGCTTCTTG 
    CTGGCTGAGC AGCTGTTCCG CCCCAAAGCG CTGGCCGAAC TGACCAAGTC TGACTGGGTG
    CACGTTGGGC GGCCCATTGT GGAGGCTCTG AGGGAGATCT CCTCCACCAC AGCCTGCTCC
    CAGCCCTTCG CCTGGAAGAA GAAAGCGCTG ATCATCATCT GGGCCAAGGT GCTACAGCCC
    TACCCCATCA CCCCTTCCGA CATGGAAACC CGCTGGCAGG AAGATGTGTT CTTCTCTGTG
    GGCAACATGA TCCCCACCAT CAATCACACA GTCCTCTTTG AGCTGCTCAA GTCGCTGGAG
    GCCTCTGGAC TCTTTATCCA GCTCCTGATG GCCCTGCCCA CCACTGTCTG CCGTGCGGAA
    CTAGAGCACT TTTTGGAGCA CATGACCATC GACACCTCTT CCAAGGACGT GGCCTTCTTC
    CTCGACGTCT GGTGGGAAAT GATGAAGCAC AAGGGCAATC AGCAGGATCC CCTGCTCTCC
    CAGTTCCGGA CAATGGCCCA CAAGTACCTG TCCTCCTCCG ATGACTTCTC CCACCCGCCG
    AAGAGGTTCA AGTCCGATCC AGATGTGTGT CCAACCATGC CCCTGCTGGC CATGCTGCTC
    ACGGGGCTGA AACAAATCCA GGACAGGATC CTATGCCCCG GGATGAAGTG CTGCGCCTTA
    GCCAACTTGG CTGACATGCT GACCGTGTTT GCGCTGATGG AGGAGGACCC CCAGGAAGTG
    TCTGCGACCG TGTACCTGGA CAAGCTGGCC ACGGTGATCT CCGTGTGGAA CTCGGACACC
    CAGAACCCGT ATCACCAGCA GGCGCTGGCA GAGAAGGTGA AGGAGGCGGA GCGGGACATC
    AGCCTGACCT CCCTGGCCAG GCTGCCGAGC GAGACCATCT TCGTGGGCTT TGAGTTCCTG
    CGCAGCCTGC TGCAGGAGTG GGGGGAGGAG CTGCAGGCCT TGCTCAGCAG CAGCCAGGGG
    ACCAGCTACG ACAGCTTCCG GCTCTGCGAC AGCCTGACCT CCTTCAGCCA GAATCTGAAG
    CTCTACCTGG ACACCACCAG CCTGGCCAAG GAGGAGAGGC AGGTGGTCTC CGAGCTGGCA
    GAGTGCGTGG GGGACTTCCT GAGGAAGACG AACAGGGTGG TGAGGAGCAA GGGCGGTGAG
    CAGGACATCA CCGCCTCCAT CGCCATGGCC ATCATCGAGC AGAAGATGGA CCGCCACATG
    GAAATGTGCT ACATTTTTGC CTCCGAGAGG AAGTGGGCCT TCTCGGACGA GTGGCTGTCC
    TGCCTGCTCA ACAACCGAGC TCTCTTCCGA GAGCCGGGCC TGGTGTTAAA GCTGCTGGAG
    ACGGTGATGG AAGTCAGCAC GATAGACAGG GCTGTCTCCG AACCTCAGAT CAAACAGGTG
    TTCGACTTGA TCCTGGAGTG TTACACACAC CTCTCTCTGC CAGATAAAAA TAAAGTCCTC
    TCGGGGGTCC TGCTCTCCTG GGGGCGCAAG GGCCTCTCTG AGAAATTGTT AGCTCACTTG
    GAGGGCTTTC AGGAGGACCT CAACACGACT TTCAACCAGC TCACACAGAG CACCTCTGAA
    CAGGGCTTGG CTAAGGCTGT TGCTTCCGTG GCCCGCCTGG TCATCCTGCA CCCAGAGATC
    ACGGTGAAGA AGTTGTGCAG CATGGCTGTG GTCAACCTCG GCACCCACAA GTTCCTGGCT
    CAGATTCTCA CTGCCTTCCC CGCTCTCCGG TTCATGGAAG AGCAGGGGCC AGACCGCTCT
    ACCACATTCG TGGTGTCCTG CCTCAAAGAA ACGGTCTGGA CCAAGCTCTC GACACCCCGG
    GAGGAGAAGC AGTTTCTGGA GCTCTTGAGC TGCCTGGTGA GTCCTACAAA GCCCCAAGGG
    ATTCCAGTCG CTGCTCTGCT CGAGCCGGAT GAGGTACTAA AGGAGTTCGT CCTGCCTTTC
    TTGATGCTGG AGGTCAAAGA GGTGGACCTC AGTCTGAGGA TCTTCGTCCA GACTCTGGAA
    GGGAGCACAA ACCTGGAAGA ATACTGGCTG CAGTCCTGCT CTCCGTTCCC GCTCATCTTC
    AGCCTGTGCC AGCTCCTTGA TGGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAGCGC
    TGTCTCCCCC TGGACGGGAA GGACCTGGCC ATCCACATCC TGGAGCTCCT CTGTGAGGTG
    GTCTCAGCTA ACACCGACAC CTTCTCCCCA GACACCTGGG TCAAGTCGCT GTCCTGGCTC
    CACCGGAAGC TGGAGCAGCT AGACTGGACT GTGGGCCTGA GACTGAAGAG CTTCTTTGAG
    GGCCACTTCA AGTGCGAGGT GCCAGCCACG CTCTTTGAGA TCTGTAAGCT TTCTGAGGAC
    GAGTGGACCT CCCAGGCCCA CCCCGGGTAT GGGCCTGGCA CAGGCCTCCT GGCCTGGATG
    GAGTGCTCCT CCATCTCCAG CAGCGTCTCC GAGCAGATGC TCGCACTCCT GGTGGTGGAC
    GTGGGCAACC CTGAGGAGGT CAGGTTGTTC AGCAAGGGCT TCCTGGTGGC CCTGGTACAG
    GTCATGCCAT GGTGCAGCCC CCAGGAATGG CAGTGCCTTC ACCAGCTGAC CCGGAGACTG
    TTGGAGAAGC AACTCCTGCA CGTCCCCTAC AGCCTGGAGT ATATACAGTT CGTTCCTCTG
    CTCAACCTGA AGCCCTTTGC CCAGGAGCTC CAGCTCTCTG TCCTCTTGCT TAGAGTTTTT
    CAGTTTCTCT GCAGCCAGAG CTGCCGTAAC TGGCTCCCTG TGGAGGGCTG GAGCCATGTG
    GTCAAGCTCC TCTGCAACAG TCTGACCAAT CTCCTGGACT CTGTTCGGTT GATACAGTCG
    GTTGGCCCTT GGGCTCAAGG ACAAGAGCAG GACCTGACCC AGGAAACCCT CTTTTTCTAT
    ACCCAGGTCT TCTGTCATGT TCTGCACATC ATGGCCATGG TCCACCAGGA GGTCTGTGAA
    CCTCTCTATG TCTTGGCCTT GGAGATCCTC ACCTGCTACG AAACCCTGAG CAAGACCAAC
    CCTTCCGTCA GCTCCTTGCT CCAGAAAGTA AACGAGCAGC GCTTCTTAAA GTCCATCGCC
    GAGAACATCA GCCCTGAGGA GCGGCGCCAA ACCCTCCTGC AGAAGATCAG CAACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002695725.1
    CDS184..3363
    Translation

    Target ORF information:

    RefSeq Version XM_002695679.4
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002695679.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACCTAG GACCTTTGAA TATCTGTGAG GAAATGACTA TCTTGCACGG GGGCTTCTTG 
    CTGGCTGAGC AGCTGTTCCG CCCCAAAGCG CTGGCCGAAC TGACCAAGTC TGACTGGGTG
    CACGTTGGGC GGCCCATTGT GGAGGCTCTG AGGGAGATCT CCTCCACCAC AGCCTGCTCC
    CAGCCCTTCG CCTGGAAGAA GAAAGCGCTG ATCATCATCT GGGCCAAGGT GCTACAGCCC
    TACCCCATCA CCCCTTCCGA CATGGAAACC CGCTGGCAGG AAGATGTGTT CTTCTCTGTG
    GGCAACATGA TCCCCACCAT CAATCACACA GTCCTCTTTG AGCTGCTCAA GTCGCTGGAG
    GCCTCTGGAC TCTTTATCCA GCTCCTGATG GCCCTGCCCA CCACTGTCTG CCGTGCGGAA
    CTAGAGCACT TTTTGGAGCA CATGACCATC GACACCTCTT CCAAGGACGT GGCCTTCTTC
    CTCGACGTCT GGTGGGAAAT GATGAAGCAC AAGGGCAATC AGCAGGATCC CCTGCTCTCC
    CAGTTCCGGA CAATGGCCCA CAAGTACCTG TCCTCCTCCG ATGACTTCTC CCACCCGCCG
    AAGAGGTTCA AGTCCGATCC AGATGTGTGT CCAACCATGC CCCTGCTGGC CATGCTGCTC
    ACGGGGCTGA AACAAATCCA GGACAGGATC CTATGCCCCG GGATGAAGTG CTGCGCCTTA
    GCCAACTTGG CTGACATGCT GACCGTGTTT GCGCTGATGG AGGAGGACCC CCAGGAAGTG
    TCTGCGACCG TGTACCTGGA CAAGCTGGCC ACGGTGATCT CCGTGTGGAA CTCGGACACC
    CAGAACCCGT ATCACCAGCA GGCGCTGGCA GAGAAGGTGA AGGAGGCGGA GCGGGACATC
    AGCCTGACCT CCCTGGCCAG GCTGCCGAGC GAGACCATCT TCGTGGGCTT TGAGTTCCTG
    CGCAGCCTGC TGCAGGAGTG GGGGGAGGAG CTGCAGGCCT TGCTCAGCAG CAGCCAGGGG
    ACCAGCTACG ACAGCTTCCG GCTCTGCGAC AGCCTGACCT CCTTCAGCCA GAATCTGAAG
    CTCTACCTGG ACACCACCAG CCTGGCCAAG GAGGAGAGGC AGGTGGTCTC CGAGCTGGCA
    GAGTGCGTGG GGGACTTCCT GAGGAAGACG AACAGGGTGG TGAGGAGCAA GGGCGGTGAG
    CAGGACATCA CCGCCTCCAT CGCCATGGCC ATCATCGAGC AGAAGATGGA CCGCCACATG
    GAAATGTGCT ACATTTTTGC CTCCGAGAGG AAGTGGGCCT TCTCGGACGA GTGGCTGTCC
    TGCCTGCTCA ACAACCGAGC TCTCTTCCGA GAGCCGGGCC TGGTGTTAAA GCTGCTGGAG
    ACGGTGATGG AAGTCAGCAC GATAGACAGG GCTGTCTCCG AACCTCAGAT CAAACAGGTG
    TTCGACTTGA TCCTGGAGTG TTACACACAC CTCTCTCTGC CAGATAAAAA TAAAGTCCTC
    TCGGGGGTCC TGCTCTCCTG GGGGCGCAAG GGCCTCTCTG AGAAATTGTT AGCTCACTTG
    GAGGGCTTTC AGGAGGACCT CAACACGACT TTCAACCAGC TCACACAGAG CACCTCTGAA
    CAGGGCTTGG CTAAGGCTGT TGCTTCCGTG GCCCGCCTGG TCATCCTGCA CCCAGAGATC
    ACGGTGAAGA AGTTGTGCAG CATGGCTGTG GTCAACCTCG GCACCCACAA GTTCCTGGCT
    CAGATTCTCA CTGCCTTCCC CGCTCTCCGG TTCATGGAAG AGCAGGGGCC AGACCGCTCT
    ACCACATTCG TGGTGTCCTG CCTCAAAGAA ACGGTCTGGA CCAAGCTCTC GACACCCCGG
    GAGGAGAAGC AGTTTCTGGA GCTCTTGAGC TGCCTGGTGA GTCCTACAAA GCCCCAAGGG
    ATTCCAGTCG CTGCTCTGCT CGAGCCGGAT GAGGTACTAA AGGAGTTCGT CCTGCCTTTC
    TTGATGCTGG AGGTCAAAGA GGTGGACCTC AGTCTGAGGA TCTTCGTCCA GACTCTGGAA
    GGGAGCACAA ACCTGGAAGA ATACTGGCTG CAGTCCTGCT CTCCGTTCCC GCTCATCTTC
    AGCCTGTGCC AGCTCCTTGA TGGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAGCGC
    TGTCTCCCCC TGGACGGGAA GGACCTGGCC ATCCACATCC TGGAGCTCCT CTGTGAGGTG
    GTCTCAGCTA ACACCGACAC CTTCTCCCCA GACACCTGGG TCAAGTCGCT GTCCTGGCTC
    CACCGGAAGC TGGAGCAGCT AGACTGGACT GTGGGCCTGA GACTGAAGAG CTTCTTTGAG
    GGCCACTTCA AGTGCGAGGT GCCAGCCACG CTCTTTGAGA TCTGTAAGCT TTCTGAGGAC
    GAGTGGACCT CCCAGGCCCA CCCCGGGTAT GGGCCTGGCA CAGGCCTCCT GGCCTGGATG
    GAGTGCTCCT CCATCTCCAG CAGCGTCTCC GAGCAGATGC TCGCACTCCT GGTGGTGGAC
    GTGGGCAACC CTGAGGAGGT CAGGTTGTTC AGCAAGGGCT TCCTGGTGGC CCTGGTACAG
    GTCATGCCAT GGTGCAGCCC CCAGGAATGG CAGTGCCTTC ACCAGCTGAC CCGGAGACTG
    TTGGAGAAGC AACTCCTGCA CGTCCCCTAC AGCCTGGAGT ATATACAGTT CGTTCCTCTG
    CTCAACCTGA AGCCCTTTGC CCAGGAGCTC CAGCTCTCTG TCCTCTTGCT TAGAGTTTTT
    CAGTTTCTCT GCAGCCAGAG CTGCCGTAAC TGGCTCCCTG TGGAGGGCTG GAGCCATGTG
    GTCAAGCTCC TCTGCAACAG TCTGACCAAT CTCCTGGACT CTGTTCGGTT GATACAGTCG
    GTTGGCCCTT GGGCTCAAGG ACAAGAGCAG GACCTGACCC AGGAAACCCT CTTTTTCTAT
    ACCCAGGTCT TCTGTCATGT TCTGCACATC ATGGCCATGG TCCACCAGGA GGTCTGTGAA
    CCTCTCTATG TCTTGGCCTT GGAGATCCTC ACCTGCTACG AAACCCTGAG CAAGACCAAC
    CCTTCCGTCA GCTCCTTGCT CCAGAAAGTA AACGAGCAGC GCTTCTTAAA GTCCATCGCC
    GAGAACATCA GCCCTGAGGA GCGGCGCCAA ACCCTCCTGC AGAAGATCAG CAACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10038
    Clone ID Related Accession (Same CDS sequence) XM_585199.8 , XM_002695679.4 , XM_002695679.5
    Accession Version XM_002695679.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product gem-associated protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002695679.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACCTAG GACCTTTGAA TATCTGTGAG GAAATGACTA TCTTGCACGG GGGCTTCTTG 
    CTGGCTGAGC AGCTGTTCCG CCCCAAAGCG CTGGCCGAAC TGACCAAGTC TGACTGGGTG
    CACGTTGGGC GGCCCATTGT GGAGGCTCTG AGGGAGATCT CCTCCACCAC AGCCTGCTCC
    CAGCCCTTCG CCTGGAAGAA GAAAGCGCTG ATCATCATCT GGGCCAAGGT GCTACAGCCC
    TACCCCATCA CCCCTTCCGA CATGGAAACC CGCTGGCAGG AAGATGTGTT CTTCTCTGTG
    GGCAACATGA TCCCCACCAT CAATCACACA GTCCTCTTTG AGCTGCTCAA GTCGCTGGAG
    GCCTCTGGAC TCTTTATCCA GCTCCTGATG GCCCTGCCCA CCACTGTCTG CCGTGCGGAA
    CTAGAGCACT TTTTGGAGCA CATGACCATC GACACCTCTT CCAAGGACGT GGCCTTCTTC
    CTCGACGTCT GGTGGGAAAT GATGAAGCAC AAGGGCAATC AGCAGGATCC CCTGCTCTCC
    CAGTTCCGGA CAATGGCCCA CAAGTACCTG TCCTCCTCCG ATGACTTCTC CCACCCGCCG
    AAGAGGTTCA AGTCCGATCC AGATGTGTGT CCAACCATGC CCCTGCTGGC CATGCTGCTC
    ACGGGGCTGA AACAAATCCA GGACAGGATC CTATGCCCCG GGATGAAGTG CTGCGCCTTA
    GCCAACTTGG CTGACATGCT GACCGTGTTT GCGCTGATGG AGGAGGACCC CCAGGAAGTG
    TCTGCGACCG TGTACCTGGA CAAGCTGGCC ACGGTGATCT CCGTGTGGAA CTCGGACACC
    CAGAACCCGT ATCACCAGCA GGCGCTGGCA GAGAAGGTGA AGGAGGCGGA GCGGGACATC
    AGCCTGACCT CCCTGGCCAG GCTGCCGAGC GAGACCATCT TCGTGGGCTT TGAGTTCCTG
    CGCAGCCTGC TGCAGGAGTG GGGGGAGGAG CTGCAGGCCT TGCTCAGCAG CAGCCAGGGG
    ACCAGCTACG ACAGCTTCCG GCTCTGCGAC AGCCTGACCT CCTTCAGCCA GAATCTGAAG
    CTCTACCTGG ACACCACCAG CCTGGCCAAG GAGGAGAGGC AGGTGGTCTC CGAGCTGGCA
    GAGTGCGTGG GGGACTTCCT GAGGAAGACG AACAGGGTGG TGAGGAGCAA GGGCGGTGAG
    CAGGACATCA CCGCCTCCAT CGCCATGGCC ATCATCGAGC AGAAGATGGA CCGCCACATG
    GAAATGTGCT ACATTTTTGC CTCCGAGAGG AAGTGGGCCT TCTCGGACGA GTGGCTGTCC
    TGCCTGCTCA ACAACCGAGC TCTCTTCCGA GAGCCGGGCC TGGTGTTAAA GCTGCTGGAG
    ACGGTGATGG AAGTCAGCAC GATAGACAGG GCTGTCTCCG AACCTCAGAT CAAACAGGTG
    TTCGACTTGA TCCTGGAGTG TTACACACAC CTCTCTCTGC CAGATAAAAA TAAAGTCCTC
    TCGGGGGTCC TGCTCTCCTG GGGGCGCAAG GGCCTCTCTG AGAAATTGTT AGCTCACTTG
    GAGGGCTTTC AGGAGGACCT CAACACGACT TTCAACCAGC TCACACAGAG CACCTCTGAA
    CAGGGCTTGG CTAAGGCTGT TGCTTCCGTG GCCCGCCTGG TCATCCTGCA CCCAGAGATC
    ACGGTGAAGA AGTTGTGCAG CATGGCTGTG GTCAACCTCG GCACCCACAA GTTCCTGGCT
    CAGATTCTCA CTGCCTTCCC CGCTCTCCGG TTCATGGAAG AGCAGGGGCC AGACCGCTCT
    ACCACATTCG TGGTGTCCTG CCTCAAAGAA ACGGTCTGGA CCAAGCTCTC GACACCCCGG
    GAGGAGAAGC AGTTTCTGGA GCTCTTGAGC TGCCTGGTGA GTCCTACAAA GCCCCAAGGG
    ATTCCAGTCG CTGCTCTGCT CGAGCCGGAT GAGGTACTAA AGGAGTTCGT CCTGCCTTTC
    TTGATGCTGG AGGTCAAAGA GGTGGACCTC AGTCTGAGGA TCTTCGTCCA GACTCTGGAA
    GGGAGCACAA ACCTGGAAGA ATACTGGCTG CAGTCCTGCT CTCCGTTCCC GCTCATCTTC
    AGCCTGTGCC AGCTCCTTGA TGGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAGCGC
    TGTCTCCCCC TGGACGGGAA GGACCTGGCC ATCCACATCC TGGAGCTCCT CTGTGAGGTG
    GTCTCAGCTA ACACCGACAC CTTCTCCCCA GACACCTGGG TCAAGTCGCT GTCCTGGCTC
    CACCGGAAGC TGGAGCAGCT AGACTGGACT GTGGGCCTGA GACTGAAGAG CTTCTTTGAG
    GGCCACTTCA AGTGCGAGGT GCCAGCCACG CTCTTTGAGA TCTGTAAGCT TTCTGAGGAC
    GAGTGGACCT CCCAGGCCCA CCCCGGGTAT GGGCCTGGCA CAGGCCTCCT GGCCTGGATG
    GAGTGCTCCT CCATCTCCAG CAGCGTCTCC GAGCAGATGC TCGCACTCCT GGTGGTGGAC
    GTGGGCAACC CTGAGGAGGT CAGGTTGTTC AGCAAGGGCT TCCTGGTGGC CCTGGTACAG
    GTCATGCCAT GGTGCAGCCC CCAGGAATGG CAGTGCCTTC ACCAGCTGAC CCGGAGACTG
    TTGGAGAAGC AACTCCTGCA CGTCCCCTAC AGCCTGGAGT ATATACAGTT CGTTCCTCTG
    CTCAACCTGA AGCCCTTTGC CCAGGAGCTC CAGCTCTCTG TCCTCTTGCT TAGAGTTTTT
    CAGTTTCTCT GCAGCCAGAG CTGCCGTAAC TGGCTCCCTG TGGAGGGCTG GAGCCATGTG
    GTCAAGCTCC TCTGCAACAG TCTGACCAAT CTCCTGGACT CTGTTCGGTT GATACAGTCG
    GTTGGCCCTT GGGCTCAAGG ACAAGAGCAG GACCTGACCC AGGAAACCCT CTTTTTCTAT
    ACCCAGGTCT TCTGTCATGT TCTGCACATC ATGGCCATGG TCCACCAGGA GGTCTGTGAA
    CCTCTCTATG TCTTGGCCTT GGAGATCCTC ACCTGCTACG AAACCCTGAG CAAGACCAAC
    CCTTCCGTCA GCTCCTTGCT CCAGAAAGTA AACGAGCAGC GCTTCTTAAA GTCCATCGCC
    GAGAACATCA GCCCTGAGGA GCGGCGCCAA ACCCTCCTGC AGAAGATCAG CAACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002695725.1
    CDS179..3358
    Translation

    Target ORF information:

    RefSeq Version XM_002695679.5
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002695679.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACCTAG GACCTTTGAA TATCTGTGAG GAAATGACTA TCTTGCACGG GGGCTTCTTG 
    CTGGCTGAGC AGCTGTTCCG CCCCAAAGCG CTGGCCGAAC TGACCAAGTC TGACTGGGTG
    CACGTTGGGC GGCCCATTGT GGAGGCTCTG AGGGAGATCT CCTCCACCAC AGCCTGCTCC
    CAGCCCTTCG CCTGGAAGAA GAAAGCGCTG ATCATCATCT GGGCCAAGGT GCTACAGCCC
    TACCCCATCA CCCCTTCCGA CATGGAAACC CGCTGGCAGG AAGATGTGTT CTTCTCTGTG
    GGCAACATGA TCCCCACCAT CAATCACACA GTCCTCTTTG AGCTGCTCAA GTCGCTGGAG
    GCCTCTGGAC TCTTTATCCA GCTCCTGATG GCCCTGCCCA CCACTGTCTG CCGTGCGGAA
    CTAGAGCACT TTTTGGAGCA CATGACCATC GACACCTCTT CCAAGGACGT GGCCTTCTTC
    CTCGACGTCT GGTGGGAAAT GATGAAGCAC AAGGGCAATC AGCAGGATCC CCTGCTCTCC
    CAGTTCCGGA CAATGGCCCA CAAGTACCTG TCCTCCTCCG ATGACTTCTC CCACCCGCCG
    AAGAGGTTCA AGTCCGATCC AGATGTGTGT CCAACCATGC CCCTGCTGGC CATGCTGCTC
    ACGGGGCTGA AACAAATCCA GGACAGGATC CTATGCCCCG GGATGAAGTG CTGCGCCTTA
    GCCAACTTGG CTGACATGCT GACCGTGTTT GCGCTGATGG AGGAGGACCC CCAGGAAGTG
    TCTGCGACCG TGTACCTGGA CAAGCTGGCC ACGGTGATCT CCGTGTGGAA CTCGGACACC
    CAGAACCCGT ATCACCAGCA GGCGCTGGCA GAGAAGGTGA AGGAGGCGGA GCGGGACATC
    AGCCTGACCT CCCTGGCCAG GCTGCCGAGC GAGACCATCT TCGTGGGCTT TGAGTTCCTG
    CGCAGCCTGC TGCAGGAGTG GGGGGAGGAG CTGCAGGCCT TGCTCAGCAG CAGCCAGGGG
    ACCAGCTACG ACAGCTTCCG GCTCTGCGAC AGCCTGACCT CCTTCAGCCA GAATCTGAAG
    CTCTACCTGG ACACCACCAG CCTGGCCAAG GAGGAGAGGC AGGTGGTCTC CGAGCTGGCA
    GAGTGCGTGG GGGACTTCCT GAGGAAGACG AACAGGGTGG TGAGGAGCAA GGGCGGTGAG
    CAGGACATCA CCGCCTCCAT CGCCATGGCC ATCATCGAGC AGAAGATGGA CCGCCACATG
    GAAATGTGCT ACATTTTTGC CTCCGAGAGG AAGTGGGCCT TCTCGGACGA GTGGCTGTCC
    TGCCTGCTCA ACAACCGAGC TCTCTTCCGA GAGCCGGGCC TGGTGTTAAA GCTGCTGGAG
    ACGGTGATGG AAGTCAGCAC GATAGACAGG GCTGTCTCCG AACCTCAGAT CAAACAGGTG
    TTCGACTTGA TCCTGGAGTG TTACACACAC CTCTCTCTGC CAGATAAAAA TAAAGTCCTC
    TCGGGGGTCC TGCTCTCCTG GGGGCGCAAG GGCCTCTCTG AGAAATTGTT AGCTCACTTG
    GAGGGCTTTC AGGAGGACCT CAACACGACT TTCAACCAGC TCACACAGAG CACCTCTGAA
    CAGGGCTTGG CTAAGGCTGT TGCTTCCGTG GCCCGCCTGG TCATCCTGCA CCCAGAGATC
    ACGGTGAAGA AGTTGTGCAG CATGGCTGTG GTCAACCTCG GCACCCACAA GTTCCTGGCT
    CAGATTCTCA CTGCCTTCCC CGCTCTCCGG TTCATGGAAG AGCAGGGGCC AGACCGCTCT
    ACCACATTCG TGGTGTCCTG CCTCAAAGAA ACGGTCTGGA CCAAGCTCTC GACACCCCGG
    GAGGAGAAGC AGTTTCTGGA GCTCTTGAGC TGCCTGGTGA GTCCTACAAA GCCCCAAGGG
    ATTCCAGTCG CTGCTCTGCT CGAGCCGGAT GAGGTACTAA AGGAGTTCGT CCTGCCTTTC
    TTGATGCTGG AGGTCAAAGA GGTGGACCTC AGTCTGAGGA TCTTCGTCCA GACTCTGGAA
    GGGAGCACAA ACCTGGAAGA ATACTGGCTG CAGTCCTGCT CTCCGTTCCC GCTCATCTTC
    AGCCTGTGCC AGCTCCTTGA TGGCTTCAGC AAATACTGGC AGCTCCCCAA GGAGAAGCGC
    TGTCTCCCCC TGGACGGGAA GGACCTGGCC ATCCACATCC TGGAGCTCCT CTGTGAGGTG
    GTCTCAGCTA ACACCGACAC CTTCTCCCCA GACACCTGGG TCAAGTCGCT GTCCTGGCTC
    CACCGGAAGC TGGAGCAGCT AGACTGGACT GTGGGCCTGA GACTGAAGAG CTTCTTTGAG
    GGCCACTTCA AGTGCGAGGT GCCAGCCACG CTCTTTGAGA TCTGTAAGCT TTCTGAGGAC
    GAGTGGACCT CCCAGGCCCA CCCCGGGTAT GGGCCTGGCA CAGGCCTCCT GGCCTGGATG
    GAGTGCTCCT CCATCTCCAG CAGCGTCTCC GAGCAGATGC TCGCACTCCT GGTGGTGGAC
    GTGGGCAACC CTGAGGAGGT CAGGTTGTTC AGCAAGGGCT TCCTGGTGGC CCTGGTACAG
    GTCATGCCAT GGTGCAGCCC CCAGGAATGG CAGTGCCTTC ACCAGCTGAC CCGGAGACTG
    TTGGAGAAGC AACTCCTGCA CGTCCCCTAC AGCCTGGAGT ATATACAGTT CGTTCCTCTG
    CTCAACCTGA AGCCCTTTGC CCAGGAGCTC CAGCTCTCTG TCCTCTTGCT TAGAGTTTTT
    CAGTTTCTCT GCAGCCAGAG CTGCCGTAAC TGGCTCCCTG TGGAGGGCTG GAGCCATGTG
    GTCAAGCTCC TCTGCAACAG TCTGACCAAT CTCCTGGACT CTGTTCGGTT GATACAGTCG
    GTTGGCCCTT GGGCTCAAGG ACAAGAGCAG GACCTGACCC AGGAAACCCT CTTTTTCTAT
    ACCCAGGTCT TCTGTCATGT TCTGCACATC ATGGCCATGG TCCACCAGGA GGTCTGTGAA
    CCTCTCTATG TCTTGGCCTT GGAGATCCTC ACCTGCTACG AAACCCTGAG CAAGACCAAC
    CCTTCCGTCA GCTCCTTGCT CCAGAAAGTA AACGAGCAGC GCTTCTTAAA GTCCATCGCC
    GAGAACATCA GCCCTGAGGA GCGGCGCCAA ACCCTCCTGC AGAAGATCAG CAACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29565
    Clone ID Related Accession (Same CDS sequence) XM_010815869.2 , XM_010824016.2
    Accession Version XM_010815869.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product gem-associated protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815869.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGGAGTG GTGTGTCCAT 
    GAAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT
    GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT
    GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC
    TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC
    ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC
    ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT
    GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG
    CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC
    GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC
    CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG
    TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG
    CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC
    TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG
    ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC
    CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG
    ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC
    CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC
    TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC
    CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC
    GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC
    ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG
    TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG
    CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG
    ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC
    TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG
    GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC
    TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC
    TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG
    AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT
    CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA
    TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG
    AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA
    GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG
    CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC
    ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG
    TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC
    CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA
    GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG
    AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC
    TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG
    ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC
    TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC
    AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG
    CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG
    AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC
    CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT
    CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG
    CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC
    CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG
    GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC
    TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC
    GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC
    ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814171.1
    CDS305..3538
    Translation

    Target ORF information:

    RefSeq Version XM_010815869.2
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815869.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGGAGTG GTGTGTCCAT 
    GAAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT
    GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT
    GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC
    TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC
    ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC
    ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT
    GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG
    CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC
    GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC
    CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG
    TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG
    CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC
    TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG
    ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC
    CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG
    ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC
    CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC
    TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC
    CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC
    GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC
    ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG
    TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG
    CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG
    ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC
    TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG
    GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC
    TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC
    TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG
    AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT
    CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA
    TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG
    AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA
    GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG
    CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC
    ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG
    TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC
    CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA
    GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG
    AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC
    TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG
    ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC
    TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC
    AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG
    CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG
    AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC
    CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT
    CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG
    CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC
    CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG
    GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC
    TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC
    GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC
    ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29565
    Clone ID Related Accession (Same CDS sequence) XM_010815869.2 , XM_010824016.2
    Accession Version XM_010824016.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product gem-associated protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGGAGTG GTGTGTCCAT 
    GAAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT
    GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT
    GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC
    TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC
    ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC
    ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT
    GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG
    CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC
    GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC
    CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG
    TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG
    CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC
    TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG
    ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC
    CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG
    ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC
    CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC
    TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC
    CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC
    GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC
    ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG
    TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG
    CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG
    ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC
    TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG
    GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC
    TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC
    TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG
    AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT
    CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA
    TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG
    AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA
    GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG
    CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC
    ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG
    TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC
    CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA
    GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG
    AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC
    TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG
    ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC
    TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC
    AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG
    CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG
    AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC
    CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT
    CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG
    CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC
    CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG
    GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC
    TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC
    GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC
    ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822318.2
    CDS305..3538
    Translation

    Target ORF information:

    RefSeq Version XM_010824016.2
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824016.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCGTGGGA CAGGAGCCCC GCTGCGGGGG AGGAAGGCTC TCAGGGAGTG GTGTGTCCAT 
    GAAGGACCTT TGAATATCTG TGAGGAAATG ACTATCTTGC ACGGGGGCTT CTTGCTGGCT
    GAGCAGCTGT TCCGCCCCAA AGCGCTGGCC GAACTGACCA AGTCTGACTG GGTGCACGTT
    GGGCGGCCCA TTGTGGAGGC TCTGAGGGAG ATCTCCTCCA CCACAGCCTG CTCCCAGCCC
    TTCGCCTGGA AGAAGAAAGC GCTGATCATC ATCTGGGCCA AGGTGCTACA GCCCTACCCC
    ATCACCCCTT CCGACATGGA AACCCGCTGG CAGGAAGATG TGTTCTTCTC TGTGGGCAAC
    ATGATCCCCA CCATCAATCA CACAGTCCTC TTTGAGCTGC TCAAGTCGCT GGAGGCCTCT
    GGACTCTTTA TCCAGCTCCT GATGGCCCTG CCCACCACTG TCTGCCGTGC GGAACTAGAG
    CACTTTTTGG AGCACATGAC CATCGACACC TCTTCCAAGG ACGTGGCCTT CTTCCTCGAC
    GTCTGGTGGG AAATGATGAA GCACAAGGGC AATCAGCAGG ATCCCCTGCT CTCCCAGTTC
    CGGACAATGG CCCACAAGTA CCTGTCCTCC TCCGATGACT TCTCCCACCC GCCGAAGAGG
    TTCAAGTCCG ATCCAGATGT GTGTCCAACC ATGCCCCTGC TGGCCATGCT GCTCACGGGG
    CTGAAACAAA TCCAGGACAG GATCCTATGC CCCGGGATGA AGTGCTGCGC CTTAGCCAAC
    TTGGCTGACA TGCTGACCGT GTTTGCGCTG ATGGAGGAGG ACCCCCAGGA AGTGTCTGCG
    ACCGTGTACC TGGACAAGCT GGCCACGGTG ATCTCCGTGT GGAACTCGGA CACCCAGAAC
    CCGTATCACC AGCAGGCGCT GGCAGAGAAG GTGAAGGAGG CGGAGCGGGA CATCAGCCTG
    ACCTCCCTGG CCAGGCTGCC GAGCGAGACC ATCTTCGTGG GCTTTGAGTT CCTGCGCAGC
    CTGCTGCAGG AGTGGGGGGA GGAGCTGCAG GCCTTGCTCA GCAGCAGCCA GGGGACCAGC
    TACGACAGCT TCCGGCTCTG CGACAGCCTG ACCTCCTTCA GCCAGAATCT GAAGCTCTAC
    CTGGACACCA CCAGCCTGGC CAAGGAGGAG AGGCAGGTGG TCTCCGAGCT GGCAGAGTGC
    GTGGGGGACT TCCTGAGGAA GACGAACAGG GTGGTGAGGA GCAAGGGCGG TGAGCAGGAC
    ATCACCGCCT CCATCGCCAT GGCCATCATC GAGCAGAAGA TGGACCGCCA CATGGAAATG
    TGCTACATTT TTGCCTCCGA GAGGAAGTGG GCCTTCTCGG ACGAGTGGCT GTCCTGCCTG
    CTCAACAACC GAGCTCTCTT CCGAGAGCCG GGCCTGGTGT TAAAGCTGCT GGAGACGGTG
    ATGGAAGTCA GCACGATAGA CAGGGCTGTC TCCGAACCTC AGATCAAACA GGTGTTCGAC
    TTGATCCTGG AGTGTTACAC ACACCTCTCT CTGCCAGATA AAAATAAAGT CCTCTCGGGG
    GTCCTGCTCT CCTGGGGGCG CAAGGGCCTC TCTGAGAAAT TGTTAGCTCA CTTGGAGGGC
    TTTCAGGAGG ACCTCAACAC GACTTTCAAC CAGCTCACAC AGAGCACCTC TGAACAGGGC
    TTGGCTAAGG CTGTTGCTTC CGTGGCCCGC CTGGTCATCC TGCACCCAGA GATCACGGTG
    AAGAAGTTGT GCAGCATGGC TGTGGTCAAC CTCGGCACCC ACAAGTTCCT GGCTCAGATT
    CTCACTGCCT TCCCCGCTCT CCGGTTCATG GAAGAGCAGG GGCCAGACCG CTCTACCACA
    TTCGTGGTGT CCTGCCTCAA AGAAACGGTC TGGACCAAGC TCTCGACACC CCGGGAGGAG
    AAGCAGTTTC TGGAGCTCTT GAGCTGCCTG GTGAGTCCTA CAAAGCCCCA AGGGATTCCA
    GTCGCTGCTC TGCTCGAGCC GGATGAGGTA CTAAAGGAGT TCGTCCTGCC TTTCTTGATG
    CTGGAGGTCA AAGAGGTGGA CCTCAGTCTG AGGATCTTCG TCCAGACTCT GGAAGGGAGC
    ACAAACCTGG AAGAATACTG GCTGCAGTCC TGCTCTCCGT TCCCGCTCAT CTTCAGCCTG
    TGCCAGCTCC TTGATGGCTT CAGCAAATAC TGGCAGCTCC CCAAGGAGAA GCGCTGTCTC
    CCCCTGGACG GGAAGGACCT GGCCATCCAC ATCCTGGAGC TCCTCTGTGA GGTGGTCTCA
    GCTAACACCG ACACCTTCTC CCCAGACACC TGGGTCAAGT CGCTGTCCTG GCTCCACCGG
    AAGCTGGAGC AGCTAGACTG GACTGTGGGC CTGAGACTGA AGAGCTTCTT TGAGGGCCAC
    TTCAAGTGCG AGGTGCCAGC CACGCTCTTT GAGATCTGTA AGCTTTCTGA GGACGAGTGG
    ACCTCCCAGG CCCACCCCGG GTATGGGCCT GGCACAGGCC TCCTGGCCTG GATGGAGTGC
    TCCTCCATCT CCAGCAGCGT CTCCGAGCAG ATGCTCGCAC TCCTGGTGGT GGACGTGGGC
    AACCCTGAGG AGGTCAGGTT GTTCAGCAAG GGCTTCCTGG TGGCCCTGGT ACAGGTCATG
    CCATGGTGCA GCCCCCAGGA ATGGCAGTGC CTTCACCAGC TGACCCGGAG ACTGTTGGAG
    AAGCAACTCC TGCACGTCCC CTACAGCCTG GAGTATATAC AGTTCGTTCC TCTGCTCAAC
    CTGAAGCCCT TTGCCCAGGA GCTCCAGCTC TCTGTCCTCT TGCTTAGAGT TTTTCAGTTT
    CTCTGCAGCC AGAGCTGCCG TAACTGGCTC CCTGTGGAGG GCTGGAGCCA TGTGGTCAAG
    CTCCTCTGCA ACAGTCTGAC CAATCTCCTG GACTCTGTTC GGTTGATACA GTCGGTTGGC
    CCTTGGGCTC AAGGACAAGA GCAGGACCTG ACCCAGGAAA CCCTCTTTTT CTATACCCAG
    GTCTTCTGTC ATGTTCTGCA CATCATGGCC ATGGTCCACC AGGAGGTCTG TGAACCTCTC
    TATGTCTTGG CCTTGGAGAT CCTCACCTGC TACGAAACCC TGAGCAAGAC CAACCCTTCC
    GTCAGCTCCT TGCTCCAGAA AGTAAACGAG CAGCGCTTCT TAAAGTCCAT CGCCGAGAAC
    ATCAGCCCTG AGGAGCGGCG CCAAACCCTC CTGCAGAAGA TCAGCAACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54209
    Clone ID Related Accession (Same CDS sequence) XM_010815870.2 , XM_010824017.2
    Accession Version XM_010815870.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product gem-associated protein 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTATCT TGCACGGGGG CTTCTTGCTG GCTGAGCAGC TGTTCCGCCC CAAAGCGCTG 
    GCCGAACTGA CCAAGTCTGA CTGGGTGCAC GTTGGGCGGC CCATTGTGGA GGCTCTGAGG
    GAGATCTCCT CCACCACAGC CTGCTCCCAG CCCTTCGCCT GGAAGAAGAA AGCGCTGATC
    ATCATCTGGG CCAAGGTGCT ACAGCCCTAC CCCATCACCC CTTCCGACAT GGAAACCCGC
    TGGCAGGAAG ATGTGTTCTT CTCTGTGGGC AACATGATCC CCACCATCAA TCACACAGTC
    CTCTTTGAGC TGCTCAAGTC GCTGGAGGCC TCTGGACTCT TTATCCAGCT CCTGATGGCC
    CTGCCCACCA CTGTCTGCCG TGCGGAACTA GAGCACTTTT TGGAGCACAT GACCATCGAC
    ACCTCTTCCA AGGACGTGGC CTTCTTCCTC GACGTCTGGT GGGAAATGAT GAAGCACAAG
    GGCAATCAGC AGGATCCCCT GCTCTCCCAG TTCCGGACAA TGGCCCACAA GTACCTGTCC
    TCCTCCGATG ACTTCTCCCA CCCGCCGAAG AGGTTCAAGT CCGATCCAGA TGTGTGTCCA
    ACCATGCCCC TGCTGGCCAT GCTGCTCACG GGGCTGAAAC AAATCCAGGA CAGGATCCTA
    TGCCCCGGGA TGAAGTGCTG CGCCTTAGCC AACTTGGCTG ACATGCTGAC CGTGTTTGCG
    CTGATGGAGG AGGACCCCCA GGAAGTGTCT GCGACCGTGT ACCTGGACAA GCTGGCCACG
    GTGATCTCCG TGTGGAACTC GGACACCCAG AACCCGTATC ACCAGCAGGC GCTGGCAGAG
    AAGGTGAAGG AGGCGGAGCG GGACATCAGC CTGACCTCCC TGGCCAGGCT GCCGAGCGAG
    ACCATCTTCG TGGGCTTTGA GTTCCTGCGC AGCCTGCTGC AGGAGTGGGG GGAGGAGCTG
    CAGGCCTTGC TCAGCAGCAG CCAGGGGACC AGCTACGACA GCTTCCGGCT CTGCGACAGC
    CTGACCTCCT TCAGCCAGAA TCTGAAGCTC TACCTGGACA CCACCAGCCT GGCCAAGGAG
    GAGAGGCAGG TGGTCTCCGA GCTGGCAGAG TGCGTGGGGG ACTTCCTGAG GAAGACGAAC
    AGGGTGGTGA GGAGCAAGGG CGGTGAGCAG GACATCACCG CCTCCATCGC CATGGCCATC
    ATCGAGCAGA AGATGGACCG CCACATGGAA ATGTGCTACA TTTTTGCCTC CGAGAGGAAG
    TGGGCCTTCT CGGACGAGTG GCTGTCCTGC CTGCTCAACA ACCGAGCTCT CTTCCGAGAG
    CCGGGCCTGG TGTTAAAGCT GCTGGAGACG GTGATGGAAG TCAGCACGAT AGACAGGGCT
    GTCTCCGAAC CTCAGATCAA ACAGGTGTTC GACTTGATCC TGGAGTGTTA CACACACCTC
    TCTCTGCCAG ATAAAAATAA AGTCCTCTCG GGGGTCCTGC TCTCCTGGGG GCGCAAGGGC
    CTCTCTGAGA AATTGTTAGC TCACTTGGAG GGCTTTCAGG AGGACCTCAA CACGACTTTC
    AACCAGCTCA CACAGAGCAC CTCTGAACAG GGCTTGGCTA AGGCTGTTGC TTCCGTGGCC
    CGCCTGGTCA TCCTGCACCC AGAGATCACG GTGAAGAAGT TGTGCAGCAT GGCTGTGGTC
    AACCTCGGCA CCCACAAGTT CCTGGCTCAG ATTCTCACTG CCTTCCCCGC TCTCCGGTTC
    ATGGAAGAGC AGGGGCCAGA CCGCTCTACC ACATTCGTGG TGTCCTGCCT CAAAGAAACG
    GTCTGGACCA AGCTCTCGAC ACCCCGGGAG GAGAAGCAGT TTCTGGAGCT CTTGAGCTGC
    CTGGTGAGTC CTACAAAGCC CCAAGGGATT CCAGTCGCTG CTCTGCTCGA GCCGGATGAG
    GTACTAAAGG AGTTCGTCCT GCCTTTCTTG ATGCTGGAGG TCAAAGAGGT GGACCTCAGT
    CTGAGGATCT TCGTCCAGAC TCTGGAAGGG AGCACAAACC TGGAAGAATA CTGGCTGCAG
    TCCTGCTCTC CGTTCCCGCT CATCTTCAGC CTGTGCCAGC TCCTTGATGG CTTCAGCAAA
    TACTGGCAGC TCCCCAAGGA GAAGCGCTGT CTCCCCCTGG ACGGGAAGGA CCTGGCCATC
    CACATCCTGG AGCTCCTCTG TGAGGTGGTC TCAGCTAACA CCGACACCTT CTCCCCAGAC
    ACCTGGGTCA AGTCGCTGTC CTGGCTCCAC CGGAAGCTGG AGCAGCTAGA CTGGACTGTG
    GGCCTGAGAC TGAAGAGCTT CTTTGAGGGC CACTTCAAGT GCGAGGTGCC AGCCACGCTC
    TTTGAGATCT GTAAGCTTTC TGAGGACGAG TGGACCTCCC AGGCCCACCC CGGGTATGGG
    CCTGGCACAG GCCTCCTGGC CTGGATGGAG TGCTCCTCCA TCTCCAGCAG CGTCTCCGAG
    CAGATGCTCG CACTCCTGGT GGTGGACGTG GGCAACCCTG AGGAGGTCAG GTTGTTCAGC
    AAGGGCTTCC TGGTGGCCCT GGTACAGGTC ATGCCATGGT GCAGCCCCCA GGAATGGCAG
    TGCCTTCACC AGCTGACCCG GAGACTGTTG GAGAAGCAAC TCCTGCACGT CCCCTACAGC
    CTGGAGTATA TACAGTTCGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCA GGAGCTCCAG
    CTCTCTGTCC TCTTGCTTAG AGTTTTTCAG TTTCTCTGCA GCCAGAGCTG CCGTAACTGG
    CTCCCTGTGG AGGGCTGGAG CCATGTGGTC AAGCTCCTCT GCAACAGTCT GACCAATCTC
    CTGGACTCTG TTCGGTTGAT ACAGTCGGTT GGCCCTTGGG CTCAAGGACA AGAGCAGGAC
    CTGACCCAGG AAACCCTCTT TTTCTATACC CAGGTCTTCT GTCATGTTCT GCACATCATG
    GCCATGGTCC ACCAGGAGGT CTGTGAACCT CTCTATGTCT TGGCCTTGGA GATCCTCACC
    TGCTACGAAA CCCTGAGCAA GACCAACCCT TCCGTCAGCT CCTTGCTCCA GAAAGTAAAC
    GAGCAGCGCT TCTTAAAGTC CATCGCCGAG AACATCAGCC CTGAGGAGCG GCGCCAAACC
    CTCCTGCAGA AGATCAGCAA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814172.1
    CDS191..3337
    Translation

    Target ORF information:

    RefSeq Version XM_010815870.2
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815870.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTATCT TGCACGGGGG CTTCTTGCTG GCTGAGCAGC TGTTCCGCCC CAAAGCGCTG 
    GCCGAACTGA CCAAGTCTGA CTGGGTGCAC GTTGGGCGGC CCATTGTGGA GGCTCTGAGG
    GAGATCTCCT CCACCACAGC CTGCTCCCAG CCCTTCGCCT GGAAGAAGAA AGCGCTGATC
    ATCATCTGGG CCAAGGTGCT ACAGCCCTAC CCCATCACCC CTTCCGACAT GGAAACCCGC
    TGGCAGGAAG ATGTGTTCTT CTCTGTGGGC AACATGATCC CCACCATCAA TCACACAGTC
    CTCTTTGAGC TGCTCAAGTC GCTGGAGGCC TCTGGACTCT TTATCCAGCT CCTGATGGCC
    CTGCCCACCA CTGTCTGCCG TGCGGAACTA GAGCACTTTT TGGAGCACAT GACCATCGAC
    ACCTCTTCCA AGGACGTGGC CTTCTTCCTC GACGTCTGGT GGGAAATGAT GAAGCACAAG
    GGCAATCAGC AGGATCCCCT GCTCTCCCAG TTCCGGACAA TGGCCCACAA GTACCTGTCC
    TCCTCCGATG ACTTCTCCCA CCCGCCGAAG AGGTTCAAGT CCGATCCAGA TGTGTGTCCA
    ACCATGCCCC TGCTGGCCAT GCTGCTCACG GGGCTGAAAC AAATCCAGGA CAGGATCCTA
    TGCCCCGGGA TGAAGTGCTG CGCCTTAGCC AACTTGGCTG ACATGCTGAC CGTGTTTGCG
    CTGATGGAGG AGGACCCCCA GGAAGTGTCT GCGACCGTGT ACCTGGACAA GCTGGCCACG
    GTGATCTCCG TGTGGAACTC GGACACCCAG AACCCGTATC ACCAGCAGGC GCTGGCAGAG
    AAGGTGAAGG AGGCGGAGCG GGACATCAGC CTGACCTCCC TGGCCAGGCT GCCGAGCGAG
    ACCATCTTCG TGGGCTTTGA GTTCCTGCGC AGCCTGCTGC AGGAGTGGGG GGAGGAGCTG
    CAGGCCTTGC TCAGCAGCAG CCAGGGGACC AGCTACGACA GCTTCCGGCT CTGCGACAGC
    CTGACCTCCT TCAGCCAGAA TCTGAAGCTC TACCTGGACA CCACCAGCCT GGCCAAGGAG
    GAGAGGCAGG TGGTCTCCGA GCTGGCAGAG TGCGTGGGGG ACTTCCTGAG GAAGACGAAC
    AGGGTGGTGA GGAGCAAGGG CGGTGAGCAG GACATCACCG CCTCCATCGC CATGGCCATC
    ATCGAGCAGA AGATGGACCG CCACATGGAA ATGTGCTACA TTTTTGCCTC CGAGAGGAAG
    TGGGCCTTCT CGGACGAGTG GCTGTCCTGC CTGCTCAACA ACCGAGCTCT CTTCCGAGAG
    CCGGGCCTGG TGTTAAAGCT GCTGGAGACG GTGATGGAAG TCAGCACGAT AGACAGGGCT
    GTCTCCGAAC CTCAGATCAA ACAGGTGTTC GACTTGATCC TGGAGTGTTA CACACACCTC
    TCTCTGCCAG ATAAAAATAA AGTCCTCTCG GGGGTCCTGC TCTCCTGGGG GCGCAAGGGC
    CTCTCTGAGA AATTGTTAGC TCACTTGGAG GGCTTTCAGG AGGACCTCAA CACGACTTTC
    AACCAGCTCA CACAGAGCAC CTCTGAACAG GGCTTGGCTA AGGCTGTTGC TTCCGTGGCC
    CGCCTGGTCA TCCTGCACCC AGAGATCACG GTGAAGAAGT TGTGCAGCAT GGCTGTGGTC
    AACCTCGGCA CCCACAAGTT CCTGGCTCAG ATTCTCACTG CCTTCCCCGC TCTCCGGTTC
    ATGGAAGAGC AGGGGCCAGA CCGCTCTACC ACATTCGTGG TGTCCTGCCT CAAAGAAACG
    GTCTGGACCA AGCTCTCGAC ACCCCGGGAG GAGAAGCAGT TTCTGGAGCT CTTGAGCTGC
    CTGGTGAGTC CTACAAAGCC CCAAGGGATT CCAGTCGCTG CTCTGCTCGA GCCGGATGAG
    GTACTAAAGG AGTTCGTCCT GCCTTTCTTG ATGCTGGAGG TCAAAGAGGT GGACCTCAGT
    CTGAGGATCT TCGTCCAGAC TCTGGAAGGG AGCACAAACC TGGAAGAATA CTGGCTGCAG
    TCCTGCTCTC CGTTCCCGCT CATCTTCAGC CTGTGCCAGC TCCTTGATGG CTTCAGCAAA
    TACTGGCAGC TCCCCAAGGA GAAGCGCTGT CTCCCCCTGG ACGGGAAGGA CCTGGCCATC
    CACATCCTGG AGCTCCTCTG TGAGGTGGTC TCAGCTAACA CCGACACCTT CTCCCCAGAC
    ACCTGGGTCA AGTCGCTGTC CTGGCTCCAC CGGAAGCTGG AGCAGCTAGA CTGGACTGTG
    GGCCTGAGAC TGAAGAGCTT CTTTGAGGGC CACTTCAAGT GCGAGGTGCC AGCCACGCTC
    TTTGAGATCT GTAAGCTTTC TGAGGACGAG TGGACCTCCC AGGCCCACCC CGGGTATGGG
    CCTGGCACAG GCCTCCTGGC CTGGATGGAG TGCTCCTCCA TCTCCAGCAG CGTCTCCGAG
    CAGATGCTCG CACTCCTGGT GGTGGACGTG GGCAACCCTG AGGAGGTCAG GTTGTTCAGC
    AAGGGCTTCC TGGTGGCCCT GGTACAGGTC ATGCCATGGT GCAGCCCCCA GGAATGGCAG
    TGCCTTCACC AGCTGACCCG GAGACTGTTG GAGAAGCAAC TCCTGCACGT CCCCTACAGC
    CTGGAGTATA TACAGTTCGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCA GGAGCTCCAG
    CTCTCTGTCC TCTTGCTTAG AGTTTTTCAG TTTCTCTGCA GCCAGAGCTG CCGTAACTGG
    CTCCCTGTGG AGGGCTGGAG CCATGTGGTC AAGCTCCTCT GCAACAGTCT GACCAATCTC
    CTGGACTCTG TTCGGTTGAT ACAGTCGGTT GGCCCTTGGG CTCAAGGACA AGAGCAGGAC
    CTGACCCAGG AAACCCTCTT TTTCTATACC CAGGTCTTCT GTCATGTTCT GCACATCATG
    GCCATGGTCC ACCAGGAGGT CTGTGAACCT CTCTATGTCT TGGCCTTGGA GATCCTCACC
    TGCTACGAAA CCCTGAGCAA GACCAACCCT TCCGTCAGCT CCTTGCTCCA GAAAGTAAAC
    GAGCAGCGCT TCTTAAAGTC CATCGCCGAG AACATCAGCC CTGAGGAGCG GCGCCAAACC
    CTCCTGCAGA AGATCAGCAA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54209
    Clone ID Related Accession (Same CDS sequence) XM_010815870.2 , XM_010824017.2
    Accession Version XM_010824017.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product gem-associated protein 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTATCT TGCACGGGGG CTTCTTGCTG GCTGAGCAGC TGTTCCGCCC CAAAGCGCTG 
    GCCGAACTGA CCAAGTCTGA CTGGGTGCAC GTTGGGCGGC CCATTGTGGA GGCTCTGAGG
    GAGATCTCCT CCACCACAGC CTGCTCCCAG CCCTTCGCCT GGAAGAAGAA AGCGCTGATC
    ATCATCTGGG CCAAGGTGCT ACAGCCCTAC CCCATCACCC CTTCCGACAT GGAAACCCGC
    TGGCAGGAAG ATGTGTTCTT CTCTGTGGGC AACATGATCC CCACCATCAA TCACACAGTC
    CTCTTTGAGC TGCTCAAGTC GCTGGAGGCC TCTGGACTCT TTATCCAGCT CCTGATGGCC
    CTGCCCACCA CTGTCTGCCG TGCGGAACTA GAGCACTTTT TGGAGCACAT GACCATCGAC
    ACCTCTTCCA AGGACGTGGC CTTCTTCCTC GACGTCTGGT GGGAAATGAT GAAGCACAAG
    GGCAATCAGC AGGATCCCCT GCTCTCCCAG TTCCGGACAA TGGCCCACAA GTACCTGTCC
    TCCTCCGATG ACTTCTCCCA CCCGCCGAAG AGGTTCAAGT CCGATCCAGA TGTGTGTCCA
    ACCATGCCCC TGCTGGCCAT GCTGCTCACG GGGCTGAAAC AAATCCAGGA CAGGATCCTA
    TGCCCCGGGA TGAAGTGCTG CGCCTTAGCC AACTTGGCTG ACATGCTGAC CGTGTTTGCG
    CTGATGGAGG AGGACCCCCA GGAAGTGTCT GCGACCGTGT ACCTGGACAA GCTGGCCACG
    GTGATCTCCG TGTGGAACTC GGACACCCAG AACCCGTATC ACCAGCAGGC GCTGGCAGAG
    AAGGTGAAGG AGGCGGAGCG GGACATCAGC CTGACCTCCC TGGCCAGGCT GCCGAGCGAG
    ACCATCTTCG TGGGCTTTGA GTTCCTGCGC AGCCTGCTGC AGGAGTGGGG GGAGGAGCTG
    CAGGCCTTGC TCAGCAGCAG CCAGGGGACC AGCTACGACA GCTTCCGGCT CTGCGACAGC
    CTGACCTCCT TCAGCCAGAA TCTGAAGCTC TACCTGGACA CCACCAGCCT GGCCAAGGAG
    GAGAGGCAGG TGGTCTCCGA GCTGGCAGAG TGCGTGGGGG ACTTCCTGAG GAAGACGAAC
    AGGGTGGTGA GGAGCAAGGG CGGTGAGCAG GACATCACCG CCTCCATCGC CATGGCCATC
    ATCGAGCAGA AGATGGACCG CCACATGGAA ATGTGCTACA TTTTTGCCTC CGAGAGGAAG
    TGGGCCTTCT CGGACGAGTG GCTGTCCTGC CTGCTCAACA ACCGAGCTCT CTTCCGAGAG
    CCGGGCCTGG TGTTAAAGCT GCTGGAGACG GTGATGGAAG TCAGCACGAT AGACAGGGCT
    GTCTCCGAAC CTCAGATCAA ACAGGTGTTC GACTTGATCC TGGAGTGTTA CACACACCTC
    TCTCTGCCAG ATAAAAATAA AGTCCTCTCG GGGGTCCTGC TCTCCTGGGG GCGCAAGGGC
    CTCTCTGAGA AATTGTTAGC TCACTTGGAG GGCTTTCAGG AGGACCTCAA CACGACTTTC
    AACCAGCTCA CACAGAGCAC CTCTGAACAG GGCTTGGCTA AGGCTGTTGC TTCCGTGGCC
    CGCCTGGTCA TCCTGCACCC AGAGATCACG GTGAAGAAGT TGTGCAGCAT GGCTGTGGTC
    AACCTCGGCA CCCACAAGTT CCTGGCTCAG ATTCTCACTG CCTTCCCCGC TCTCCGGTTC
    ATGGAAGAGC AGGGGCCAGA CCGCTCTACC ACATTCGTGG TGTCCTGCCT CAAAGAAACG
    GTCTGGACCA AGCTCTCGAC ACCCCGGGAG GAGAAGCAGT TTCTGGAGCT CTTGAGCTGC
    CTGGTGAGTC CTACAAAGCC CCAAGGGATT CCAGTCGCTG CTCTGCTCGA GCCGGATGAG
    GTACTAAAGG AGTTCGTCCT GCCTTTCTTG ATGCTGGAGG TCAAAGAGGT GGACCTCAGT
    CTGAGGATCT TCGTCCAGAC TCTGGAAGGG AGCACAAACC TGGAAGAATA CTGGCTGCAG
    TCCTGCTCTC CGTTCCCGCT CATCTTCAGC CTGTGCCAGC TCCTTGATGG CTTCAGCAAA
    TACTGGCAGC TCCCCAAGGA GAAGCGCTGT CTCCCCCTGG ACGGGAAGGA CCTGGCCATC
    CACATCCTGG AGCTCCTCTG TGAGGTGGTC TCAGCTAACA CCGACACCTT CTCCCCAGAC
    ACCTGGGTCA AGTCGCTGTC CTGGCTCCAC CGGAAGCTGG AGCAGCTAGA CTGGACTGTG
    GGCCTGAGAC TGAAGAGCTT CTTTGAGGGC CACTTCAAGT GCGAGGTGCC AGCCACGCTC
    TTTGAGATCT GTAAGCTTTC TGAGGACGAG TGGACCTCCC AGGCCCACCC CGGGTATGGG
    CCTGGCACAG GCCTCCTGGC CTGGATGGAG TGCTCCTCCA TCTCCAGCAG CGTCTCCGAG
    CAGATGCTCG CACTCCTGGT GGTGGACGTG GGCAACCCTG AGGAGGTCAG GTTGTTCAGC
    AAGGGCTTCC TGGTGGCCCT GGTACAGGTC ATGCCATGGT GCAGCCCCCA GGAATGGCAG
    TGCCTTCACC AGCTGACCCG GAGACTGTTG GAGAAGCAAC TCCTGCACGT CCCCTACAGC
    CTGGAGTATA TACAGTTCGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCA GGAGCTCCAG
    CTCTCTGTCC TCTTGCTTAG AGTTTTTCAG TTTCTCTGCA GCCAGAGCTG CCGTAACTGG
    CTCCCTGTGG AGGGCTGGAG CCATGTGGTC AAGCTCCTCT GCAACAGTCT GACCAATCTC
    CTGGACTCTG TTCGGTTGAT ACAGTCGGTT GGCCCTTGGG CTCAAGGACA AGAGCAGGAC
    CTGACCCAGG AAACCCTCTT TTTCTATACC CAGGTCTTCT GTCATGTTCT GCACATCATG
    GCCATGGTCC ACCAGGAGGT CTGTGAACCT CTCTATGTCT TGGCCTTGGA GATCCTCACC
    TGCTACGAAA CCCTGAGCAA GACCAACCCT TCCGTCAGCT CCTTGCTCCA GAAAGTAAAC
    GAGCAGCGCT TCTTAAAGTC CATCGCCGAG AACATCAGCC CTGAGGAGCG GCGCCAAACC
    CTCCTGCAGA AGATCAGCAA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822319.1
    CDS191..3337
    Translation

    Target ORF information:

    RefSeq Version XM_010824017.2
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 4 (GEMIN4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824017.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTATCT TGCACGGGGG CTTCTTGCTG GCTGAGCAGC TGTTCCGCCC CAAAGCGCTG 
    GCCGAACTGA CCAAGTCTGA CTGGGTGCAC GTTGGGCGGC CCATTGTGGA GGCTCTGAGG
    GAGATCTCCT CCACCACAGC CTGCTCCCAG CCCTTCGCCT GGAAGAAGAA AGCGCTGATC
    ATCATCTGGG CCAAGGTGCT ACAGCCCTAC CCCATCACCC CTTCCGACAT GGAAACCCGC
    TGGCAGGAAG ATGTGTTCTT CTCTGTGGGC AACATGATCC CCACCATCAA TCACACAGTC
    CTCTTTGAGC TGCTCAAGTC GCTGGAGGCC TCTGGACTCT TTATCCAGCT CCTGATGGCC
    CTGCCCACCA CTGTCTGCCG TGCGGAACTA GAGCACTTTT TGGAGCACAT GACCATCGAC
    ACCTCTTCCA AGGACGTGGC CTTCTTCCTC GACGTCTGGT GGGAAATGAT GAAGCACAAG
    GGCAATCAGC AGGATCCCCT GCTCTCCCAG TTCCGGACAA TGGCCCACAA GTACCTGTCC
    TCCTCCGATG ACTTCTCCCA CCCGCCGAAG AGGTTCAAGT CCGATCCAGA TGTGTGTCCA
    ACCATGCCCC TGCTGGCCAT GCTGCTCACG GGGCTGAAAC AAATCCAGGA CAGGATCCTA
    TGCCCCGGGA TGAAGTGCTG CGCCTTAGCC AACTTGGCTG ACATGCTGAC CGTGTTTGCG
    CTGATGGAGG AGGACCCCCA GGAAGTGTCT GCGACCGTGT ACCTGGACAA GCTGGCCACG
    GTGATCTCCG TGTGGAACTC GGACACCCAG AACCCGTATC ACCAGCAGGC GCTGGCAGAG
    AAGGTGAAGG AGGCGGAGCG GGACATCAGC CTGACCTCCC TGGCCAGGCT GCCGAGCGAG
    ACCATCTTCG TGGGCTTTGA GTTCCTGCGC AGCCTGCTGC AGGAGTGGGG GGAGGAGCTG
    CAGGCCTTGC TCAGCAGCAG CCAGGGGACC AGCTACGACA GCTTCCGGCT CTGCGACAGC
    CTGACCTCCT TCAGCCAGAA TCTGAAGCTC TACCTGGACA CCACCAGCCT GGCCAAGGAG
    GAGAGGCAGG TGGTCTCCGA GCTGGCAGAG TGCGTGGGGG ACTTCCTGAG GAAGACGAAC
    AGGGTGGTGA GGAGCAAGGG CGGTGAGCAG GACATCACCG CCTCCATCGC CATGGCCATC
    ATCGAGCAGA AGATGGACCG CCACATGGAA ATGTGCTACA TTTTTGCCTC CGAGAGGAAG
    TGGGCCTTCT CGGACGAGTG GCTGTCCTGC CTGCTCAACA ACCGAGCTCT CTTCCGAGAG
    CCGGGCCTGG TGTTAAAGCT GCTGGAGACG GTGATGGAAG TCAGCACGAT AGACAGGGCT
    GTCTCCGAAC CTCAGATCAA ACAGGTGTTC GACTTGATCC TGGAGTGTTA CACACACCTC
    TCTCTGCCAG ATAAAAATAA AGTCCTCTCG GGGGTCCTGC TCTCCTGGGG GCGCAAGGGC
    CTCTCTGAGA AATTGTTAGC TCACTTGGAG GGCTTTCAGG AGGACCTCAA CACGACTTTC
    AACCAGCTCA CACAGAGCAC CTCTGAACAG GGCTTGGCTA AGGCTGTTGC TTCCGTGGCC
    CGCCTGGTCA TCCTGCACCC AGAGATCACG GTGAAGAAGT TGTGCAGCAT GGCTGTGGTC
    AACCTCGGCA CCCACAAGTT CCTGGCTCAG ATTCTCACTG CCTTCCCCGC TCTCCGGTTC
    ATGGAAGAGC AGGGGCCAGA CCGCTCTACC ACATTCGTGG TGTCCTGCCT CAAAGAAACG
    GTCTGGACCA AGCTCTCGAC ACCCCGGGAG GAGAAGCAGT TTCTGGAGCT CTTGAGCTGC
    CTGGTGAGTC CTACAAAGCC CCAAGGGATT CCAGTCGCTG CTCTGCTCGA GCCGGATGAG
    GTACTAAAGG AGTTCGTCCT GCCTTTCTTG ATGCTGGAGG TCAAAGAGGT GGACCTCAGT
    CTGAGGATCT TCGTCCAGAC TCTGGAAGGG AGCACAAACC TGGAAGAATA CTGGCTGCAG
    TCCTGCTCTC CGTTCCCGCT CATCTTCAGC CTGTGCCAGC TCCTTGATGG CTTCAGCAAA
    TACTGGCAGC TCCCCAAGGA GAAGCGCTGT CTCCCCCTGG ACGGGAAGGA CCTGGCCATC
    CACATCCTGG AGCTCCTCTG TGAGGTGGTC TCAGCTAACA CCGACACCTT CTCCCCAGAC
    ACCTGGGTCA AGTCGCTGTC CTGGCTCCAC CGGAAGCTGG AGCAGCTAGA CTGGACTGTG
    GGCCTGAGAC TGAAGAGCTT CTTTGAGGGC CACTTCAAGT GCGAGGTGCC AGCCACGCTC
    TTTGAGATCT GTAAGCTTTC TGAGGACGAG TGGACCTCCC AGGCCCACCC CGGGTATGGG
    CCTGGCACAG GCCTCCTGGC CTGGATGGAG TGCTCCTCCA TCTCCAGCAG CGTCTCCGAG
    CAGATGCTCG CACTCCTGGT GGTGGACGTG GGCAACCCTG AGGAGGTCAG GTTGTTCAGC
    AAGGGCTTCC TGGTGGCCCT GGTACAGGTC ATGCCATGGT GCAGCCCCCA GGAATGGCAG
    TGCCTTCACC AGCTGACCCG GAGACTGTTG GAGAAGCAAC TCCTGCACGT CCCCTACAGC
    CTGGAGTATA TACAGTTCGT TCCTCTGCTC AACCTGAAGC CCTTTGCCCA GGAGCTCCAG
    CTCTCTGTCC TCTTGCTTAG AGTTTTTCAG TTTCTCTGCA GCCAGAGCTG CCGTAACTGG
    CTCCCTGTGG AGGGCTGGAG CCATGTGGTC AAGCTCCTCT GCAACAGTCT GACCAATCTC
    CTGGACTCTG TTCGGTTGAT ACAGTCGGTT GGCCCTTGGG CTCAAGGACA AGAGCAGGAC
    CTGACCCAGG AAACCCTCTT TTTCTATACC CAGGTCTTCT GTCATGTTCT GCACATCATG
    GCCATGGTCC ACCAGGAGGT CTGTGAACCT CTCTATGTCT TGGCCTTGGA GATCCTCACC
    TGCTACGAAA CCCTGAGCAA GACCAACCCT TCCGTCAGCT CCTTGCTCCA GAAAGTAAAC
    GAGCAGCGCT TCTTAAAGTC CATCGCCGAG AACATCAGCC CTGAGGAGCG GCGCCAAACC
    CTCCTGCAGA AGATCAGCAA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.