KMT2E gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005205362.3	XP_005205419.1	histone-lysine N-methyltransferase 2E isoform X1
XM_005205366.4	XP_005205423.1	inactive histone-lysine N-methyltransferase 2E isoform X2
XM_024990819.1	XP_024846587.1	inactive histone-lysine N-methyltransferase 2E isoform X4
XM_005205363.3	XP_005205420.1	histone-lysine N-methyltransferase 2E isoform X1
XM_005205366.3	XP_005205423.1	histone-lysine N-methyltransferase 2E isoform X2
NM_001082451.2	NP_001075920.2	histone-lysine N-methyltransferase 2E
XM_005205364.3	XP_005205421.1	histone-lysine N-methyltransferase 2E isoform X1
XM_005205363.4	XP_005205420.1	inactive histone-lysine N-methyltransferase 2E isoform X1
XM_005205365.4	XP_005205422.1	inactive histone-lysine N-methyltransferase 2E isoform X1
XM_005205367.4	XP_005205424.1	inactive histone-lysine N-methyltransferase 2E isoform X3
XM_005205365.3	XP_005205422.1	histone-lysine N-methyltransferase 2E isoform X1
XM_005205367.3	XP_005205424.1	histone-lysine N-methyltransferase 2E isoform X3
XM_005205362.4	XP_005205419.1	inactive histone-lysine N-methyltransferase 2E isoform X1
NM_001082451.3	NP_001075920.2	inactive histone-lysine N-methyltransferase 2E
XM_005205364.4	XP_005205421.1	inactive histone-lysine N-methyltransferase 2E isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa233777	XM_005205366.4 Latest version!	Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233778	XM_005205367.4 Latest version!	Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233779	XM_024990819.1 Latest version!	Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa01901	NM_001082451.3 Latest version!	Bos taurus lysine methyltransferase 2E (KMT2E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa01901	XM_005205364.4 Latest version!	Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa01901	XM_005205365.4 Latest version!	Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa01901	XM_005205363.4 Latest version!	Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa01901	XM_005205362.4 Latest version!	Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18519	XM_005205364.3 Latest version!	Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18519	XM_005205363.3 Latest version!	Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18519	XM_005205362.3 Latest version!	Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18519	XM_005205365.3 Latest version!	Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18520	XM_005205366.3 Latest version!	Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18521	XM_005205367.3 Latest version!	Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OBa01901	NM_001082451.2	Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

KMT2E ( XM_005205366.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205423.1 Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa233777
Clone ID Related Accession (Same CDS sequence)	XM_005205366.4
Accession Version	XM_005205366.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive histone-lysine N-methyltransferase 2E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205366.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGATCCTG ATCCTGAAAA TCTAGAACCC ACAACTACAA ATGAATGCCC ATCCCCAGAT 
ACTTCCCAAA GTACTTGTAA AAGTCCTTCA AAAATGAGCA AGCCTGGTTC ACCTGGACCT 
GTAATTCCTG TTCAGCCACA TGGGAAAATA CTTACAAAAC CAGATTCCCA TTGGGAGGGA 
ACAGTTATTG TATCTGAAGC TGAGAATGGT GTCCACCTGA AAACAGAACT CCATCAAAAA 
CAGCTGTCAA GTAACCCCCA AGCACTTTCA AAGAACCATC CTCCACAGCC ACTCGTTCGC 
TGTTCATCTG AGCAGCTTTC ACAAAAGCTG CCTTCTGCAC CCATGAAGTT GCACTGTCCT 
CCGTCACCTC ACGTAGAAAA TCCTCCAAAG TCATCGACGC CTCACACGCC TGTACAGCAT 
GGCTATCTCT CACCAAAGCC GCCTACACAG CAGTTAGGCT CTCCCTATAG GCCTCACCAT 
TCACAGTCAC CTCAAGTTGG AACACCTCAG CGAGAGCCTC AAAGAAATTT TTACCCAGCC 
GCACAGAACC TTCAAGCCAG TACTCAGCAG GCAACTTCTG GAGCATTATT TACACAGACA 
CCCTCAGGAC AATCTTCAGC AACATACAGT CAGTTTAATC AGACAAGTCT GAGCAGCACG 
GCACCACCCC CTCCACCCCC TCCACCCCCT TCTTCTTCGT CTTATTATCA AAACCAGCAG 
CCCTCTGCAA ACTTTCAGAA TTATAATCAG CTTAAAGGTA GTCTTTCCCA ACAAACTGTG 
TTTACATCAG GACCAAATCA AGCACTTCCT GGCACCACAA GCCAGCAAAC AGTTCCAGGA 
CACCACGTTA CTCCAGGGCA TTTTTTGCCT TCTCAGAACC CTACTGTTCA CCATCAGACT 
GCTGCTGCTG TGGTCCCACC CCCTCCTCCA CCCCCACCTG CTCCAGGACC ACACCTCGTA 
CAACAGCCAA GCTCCCATCA GCCACACTCA GTAGCACATG TGGTGGGGCC TGTTCACGCT 
GTCACCCCTG GATCGCATAT TCATTCTCAA ACTGCTGGGC ACCATTTGCC ACCACCGCCT 
CCCCCTCCTG GTCCTGCCCC TCATCACCAC CCCCCGCCCC ACCCTTCCAC AGGACTCCAA 
GGTCTACAAG CACAACACCA GCATGTGGTA AACTCAGCCC CTCCACCTCC CCCTCCTCCT 
CCGCCTTCCA GTGTTCTGGC TTCTGGGCAT CACACCACGT CAGCTCAAGC CTTACACCAT 
CCACCTCATC AAGGACCGCC ACTTTTTCCT TCAAGTGCTC ATCCAGCTGT ACCACCGTAT 
CCCTCACAAG CTACACATCA CACCACTCTG GGACTGGGAC CCCAACACCA GCCTTCTGGA 
ACAGGGCCAC ATTGTCCATT ACCAGTTGCA GGTCCTCATC TCCAGCCCCA AGGACCAAAC 
AGTATTCCAA CACCTACCGC TTCAGGGTTC TGTCCTCACC CTGGCTCTGT GGCCCTGCCA 
CATGGGGTCC AAGGACCTCA GCAGGCATCT CCAGTGCCTG GACAGATTCC AATTCACAGA 
GCACAGGTGC CACCGACATT TCAGAACAAT TACCATGGTT CGGGGTGGCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205423.1
CDS	510..5963
Translation	MSIVIPLGVDTAETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSVLISKNEVSIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_005205366.4
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205366.4

ORF Insert Sequence:

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGATCCTG ATCCTGAAAA TCTAGAACCC ACAACTACAA ATGAATGCCC ATCCCCAGAT 
ACTTCCCAAA GTACTTGTAA AAGTCCTTCA AAAATGAGCA AGCCTGGTTC ACCTGGACCT 
GTAATTCCTG TTCAGCCACA TGGGAAAATA CTTACAAAAC CAGATTCCCA TTGGGAGGGA 
ACAGTTATTG TATCTGAAGC TGAGAATGGT GTCCACCTGA AAACAGAACT CCATCAAAAA 
CAGCTGTCAA GTAACCCCCA AGCACTTTCA AAGAACCATC CTCCACAGCC ACTCGTTCGC 
TGTTCATCTG AGCAGCTTTC ACAAAAGCTG CCTTCTGCAC CCATGAAGTT GCACTGTCCT 
CCGTCACCTC ACGTAGAAAA TCCTCCAAAG TCATCGACGC CTCACACGCC TGTACAGCAT 
GGCTATCTCT CACCAAAGCC GCCTACACAG CAGTTAGGCT CTCCCTATAG GCCTCACCAT 
TCACAGTCAC CTCAAGTTGG AACACCTCAG CGAGAGCCTC AAAGAAATTT TTACCCAGCC 
GCACAGAACC TTCAAGCCAG TACTCAGCAG GCAACTTCTG GAGCATTATT TACACAGACA 
CCCTCAGGAC AATCTTCAGC AACATACAGT CAGTTTAATC AGACAAGTCT GAGCAGCACG 
GCACCACCCC CTCCACCCCC TCCACCCCCT TCTTCTTCGT CTTATTATCA AAACCAGCAG 
CCCTCTGCAA ACTTTCAGAA TTATAATCAG CTTAAAGGTA GTCTTTCCCA ACAAACTGTG 
TTTACATCAG GACCAAATCA AGCACTTCCT GGCACCACAA GCCAGCAAAC AGTTCCAGGA 
CACCACGTTA CTCCAGGGCA TTTTTTGCCT TCTCAGAACC CTACTGTTCA CCATCAGACT 
GCTGCTGCTG TGGTCCCACC CCCTCCTCCA CCCCCACCTG CTCCAGGACC ACACCTCGTA 
CAACAGCCAA GCTCCCATCA GCCACACTCA GTAGCACATG TGGTGGGGCC TGTTCACGCT 
GTCACCCCTG GATCGCATAT TCATTCTCAA ACTGCTGGGC ACCATTTGCC ACCACCGCCT 
CCCCCTCCTG GTCCTGCCCC TCATCACCAC CCCCCGCCCC ACCCTTCCAC AGGACTCCAA 
GGTCTACAAG CACAACACCA GCATGTGGTA AACTCAGCCC CTCCACCTCC CCCTCCTCCT 
CCGCCTTCCA GTGTTCTGGC TTCTGGGCAT CACACCACGT CAGCTCAAGC CTTACACCAT 
CCACCTCATC AAGGACCGCC ACTTTTTCCT TCAAGTGCTC ATCCAGCTGT ACCACCGTAT 
CCCTCACAAG CTACACATCA CACCACTCTG GGACTGGGAC CCCAACACCA GCCTTCTGGA 
ACAGGGCCAC ATTGTCCATT ACCAGTTGCA GGTCCTCATC TCCAGCCCCA AGGACCAAAC 
AGTATTCCAA CACCTACCGC TTCAGGGTTC TGTCCTCACC CTGGCTCTGT GGCCCTGCCA 
CATGGGGTCC AAGGACCTCA GCAGGCATCT CCAGTGCCTG GACAGATTCC AATTCACAGA 
GCACAGGTGC CACCGACATT TCAGAACAAT TACCATGGTT CGGGGTGGCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KMT2E ( XM_005205367.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205424.1 Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X6, mRNA.

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CloneID	OBa233778
Clone ID Related Accession (Same CDS sequence)	XM_005205367.4
Accession Version	XM_005205367.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive histone-lysine N-methyltransferase 2E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205367.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGATTG ACAGGCAGCA TATTCCTGAT ACATATCTTT GTGAACGTTG TCAGCCTAGG 
AGTTTGGATA AAGAGAGGGC GGTGCTACTA CAACGCCGGA AAAGGGAAAA TATGTCAGAT 
GGTGATACCA GTGCAACTGA GAGTGGTGAT GAGGTTCCTG TGGAATTATA TACTGCATTT 
CAGCATACCC CAACATCGAT TACTTTAACT GCTTCAAGGG TTTCTAAGGT TAATGATAAA 
AGAAGGAAAA AAAGTGGGGA GAAAGAACAA AATATTTCAA AATGTAAAAA AGCCTTTCGT 
GAAGGATCTA GGAAATCATC AAGAGTTAAG GGCTCAGCTC CGGAGATTGA TCCTTCATCT 
GATGTTTCAA ATTTTGGATG GGAGACAAAA ATCAAAGCAT GGATGGATCA ATATGAAGAG 
GCAAATAATA ACCAATATAG TGAGAGTGTT CAGAGGGAGG CACAAAGAAT AGCTCTGAGA 
TTGGGCAATG GAAATGACAA AAAAGAGATT AAATCAGATT TGAATACCAA CAATTTGATC 
TTCAAACCTC CTGTAGAGAG CCATATACAA AAAAATAAGA AGATTCTGAA ATCTGCAAAA 
GATTTGCCTC CTGATGCACT TATCATTGAA TACAGAGGGA AGTTTATGTT GAGAGAGCAG 
TTTGAAGCAA ATGGATATTT CTTTAAGAGA CCATACCCTT TTGTTTTATT CTATTCTAAA 
TTTCATGGCC TAGAAATGTG TGTTGATGCA AGGACTTTTG GGAATGAAGC TCGGTTCATC 
AGGCGTTCTT GTACACCCAA TGCAGAGGTG AGGCATGAAA TTGAAGATGG AACCATACAT 
CTTTATATTT ATTCTATACA AAGTATTCCA AAGGGAACCG AAATTACTAT TGCCTTTGAT 
TTTGACTATG GGAATTGTAA GTACAAGGTG GATTGTGCAT GCCTCAAAGA AAATTCAGAG 
TGCCCTGTTC TGAAACGTAG TTCTGAATCC ACGGAGAACA TCAATAGCGG TTATGAGACC 
AGAAGGAAGA AAGGCAAAAA AGAAAAGGAT ATTTCAAAAG AAAAAGACAC ACAAAATCAG 
AATATTACTC TGGATTGTGA AGGAACAACG AACAAAATGA AGAGCCCAGA AACTAAACAG 
AGAAAGCTTT CTCCACTGAG ACTATCAGTA TCAAATAATC AGGAACCAGA TTTTATTGAT 
GATATAGAAG AAAAAACTCC CATCAGCAAT GAGGTAGAAA TGGAGTCTGA GGAACAGATT 
GCAGAGAGGA AAAGGAAGAT GACAAGAGAA GAAAGGAAAA TGGAAGCAAT TCTGCAGGCT 
TTTGCCAGAC TTGAAAAAAG AGAGAAGAGA AGAGAACAAG CTTTGGAAAG AATCAGCACA 
GCCAAAACTG AAGTTAAAAC TGAATGTAAA GATGCACAGA CTGTCAGTGA TGCTGAAGTT 
ATTCAGGAAC AAGCAAAAGA AGAAACTGCT AGCAAGCCAA CCCCAGCCAA AGTAAATAGA 
ACTAAACAGA GAAAAAGTTT TTCTCGGAGC AGGACTCACA TTGGACAGCA GCGTCGGAGA 
CACAGGACTG TCAGTATGTG TTCGGATATC CAACCGTCTT CTCCTGACAT CGAAGTTACC 
TCACAACAAA ACGATGTGGA AAATACTGTA CTTGCAATAG AACCAGAAAC TGAAACTGCA 
CTAACAGAAA TAATTACTGA AAATGAAGTT CCAGTACTTA ATAAATGTCC AACAAAGTAC 
CCTAAAACAA AGAAGCACTT GGTCAGTGAA TGGTTAAGTG AGAAGAATGA GAAGACAGGA 
AAACCTTCAG ACAGCCTTTC AGAGAGGCCG CTGCGCATAA CTACAGATCC TGAAGTGCTA 
GCTACACAGC TCAATTCTTT GCCAGGTCTC ACTTACAGTC CACATGTATA CTCTACTCCT 
AAGCACTATA TTAGATTCAC TTCACCATTC CTTTCGGAAA AAAGGAGAAG AAAAGAACCT 
CCTGAAAACA TATCTGGCTC ATGCAAGAAG CGGTGGTTGA AACAAGCTCT GGAAGAGGAA 
AACTCAGCAA TTTTACATAG ATTTAATTCG CCCTGTCAAG AAAGATCCAG AAGTCCTACA 
GTCAATGGTG AAAATAAAAG TCCACTACTA TTAAATGATA GCTGTTCCCT TCCAGATTTA 
ACTACACCAC TGAAAAAACG AAGACTTTAT CAGTTGCTAG ATTCTGCTTA CTCAGAAACC 
TCCACACCTA CTCCTTCACC GTATGCTACA CCAACTCACA CAGATATTAC TCCTACAGAC 
CCATCATTTT CCACTCCTCC ACGGATAAAG TCAGATGATG AAGCTTGTAG AAATGGTTAT 
AAGCCCATAT ATTCACCAGT TACCCCAGTA ACTCCTGGCA CACCAGGAAA TACCATGCAC 
TTTGAGAATA TTTCTTCCCC AGAAAGTTCT CCAGAAATTA AGAGACGCAC TTATAGTCAA 
GAGGGATATG ACAGATCGTC AACCATGTTA ACGTTGGGGC CTTTTAGAAA TTCTAACTTA 
ACTGAACTGG GTCTGCAAGA AATAAAGACT ATTGGCTATG GCAGCCCTAG GAGTAGGACT 
GAAATCAGCA GGCAGTGCCC TGGAGAAAAG GAACCGGTGT CAGATCTTCA ACTGGGACTC 
GATGCAGTCG AGCAGACCGC CTTACATAAA ACCATGGAAG CAGCTGCACA CGACAGGACT 
GAGGCTGCCA GCCAGCTGGA GGCTGCTCAC TCGGGGCGGG GCCCTCTGTA TTCCTCCTGG 
GTAAAGAGTC CTGAGAGAAC AGGCGTTAAC TTCTCAGTAA ATTCCAACTT GAGGGACCTG 
ACACCCTCAC ATCAGTTGGA GGTTGGAGGC GGCTTCCGAA TAAGTGAGTC AAAGTGCCTG 
ATGCAGGATG ATACTAGAGG CATGTTTATG GAAACACCCG TGTTTTGTAC TTCAGAAGAT 
GGGCTTGTAT CTGGTTTCGG ACGGACTGTT AATGACAATT TGATCGACGG AAGTTGCACA 
CCCCAGAATC CACCACAAAA GAAAAAGGTT TCTCTATTAG AATACCGGAA GAGACAACGT 
GAAGCTAGGA AGAGCGGCTC TAAGACGGAG CACTTTGCGC TGGTTAGTGT ATCACCTCAC 
ACAAGTGGCA ACTTGAGCAG CAGCACTGGT GATGGGTGTG CCCACAGGAG TGAAAATGGG 
GAGCAGGTAG AAAGCGCTGC TAGCCTACCT TTACCAACAC CAGCTACAGT TTATCATGCT 
ACTTCGGAAG AAACCAGCAA TAACAGTACT GTTAAGGAAG TGTCTGCCAG TGAAAAGAAT 
GAACCAGAAG TTCAATGGAC GGCCTCAACT TCAGTGGAGC AAGTGAGAGA GAGGAGTTAT 
CAGAGAGCTC TGCTTCTCAG TGATCACCGA AAAGATAAAG ACAGTGGGGC AGAGTCACCA 
TGTGTCCCCT GTTCACCGAA TCATGTTCCG TCTTCTCCTT CATCTCATTC AAATCACATT 
CCCCAGTTGC AACCCAAGGG CCCAGTCCCT TCTTTCAGTG AACTTACGGA AGATCCTGAT 
CCTGAAAATC TAGAACCCAC AACTACAAAT GAATGCCCAT CCCCAGATAC TTCCCAAAGT 
ACTTGTAAAA GTCCTTCAAA AATGAGCAAG CCTGGTTCAC CTGGACCTGT AATTCCTGTT 
CAGCCACATG GGAAAATACT TACAAAACCA GATTCCCATT GGGAGGGAAC AGTTATTGTA 
TCTGAAGCTG AGAATGGTGT CCACCTGAAA ACAGAACTCC ATCAAAAACA GCTGTCAAGT 
AACCCCCAAG CACTTTCAAA GAACCATCCT CCACAGCCAC TCGTTCGCTG TTCATCTGAG 
CAGCTTTCAC AAAAGCTGCC TTCTGCACCC ATGAAGTTGC ACTGTCCTCC GTCACCTCAC 
GTAGAAAATC CTCCAAAGTC ATCGACGCCT CACACGCCTG TACAGCATGG CTATCTCTCA 
CCAAAGCCGC CTACACAGCA GTTAGGCTCT CCCTATAGGC CTCACCATTC ACAGTCACCT 
CAAGTTGGAA CACCTCAGCG AGAGCCTCAA AGAAATTTTT ACCCAGCCGC ACAGAACCTT 
CAAGCCAGTA CTCAGCAGGC AACTTCTGGA GCATTATTTA CACAGACACC CTCAGGACAA 
TCTTCAGCAA CATACAGTCA GTTTAATCAG ACAAGTCTGA GCAGCACGGC ACCACCCCCT 
CCACCCCCTC CACCCCCTTC TTCTTCGTCT TATTATCAAA ACCAGCAGCC CTCTGCAAAC 
TTTCAGAATT ATAATCAGCT TAAAGGTAGT CTTTCCCAAC AAACTGTGTT TACATCAGGA 
CCAAATCAAG CACTTCCTGG CACCACAAGC CAGCAAACAG TTCCAGGACA CCACGTTACT 
CCAGGGCATT TTTTGCCTTC TCAGAACCCT ACTGTTCACC ATCAGACTGC TGCTGCTGTG 
GTCCCACCCC CTCCTCCACC CCCACCTGCT CCAGGACCAC ACCTCGTACA ACAGCCAAGC 
TCCCATCAGC CACACTCAGT AGCACATGTG GTGGGGCCTG TTCACGCTGT CACCCCTGGA 
TCGCATATTC ATTCTCAAAC TGCTGGGCAC CATTTGCCAC CACCGCCTCC CCCTCCTGGT 
CCTGCCCCTC ATCACCACCC CCCGCCCCAC CCTTCCACAG GACTCCAAGG TCTACAAGCA 
CAACACCAGC ATGTGGTAAA CTCAGCCCCT CCACCTCCCC CTCCTCCTCC GCCTTCCAGT 
GTTCTGGCTT CTGGGCATCA CACCACGTCA GCTCAAGCCT TACACCATCC ACCTCATCAA 
GGACCGCCAC TTTTTCCTTC AAGTGCTCAT CCAGCTGTAC CACCGTATCC CTCACAAGCT 
ACACATCACA CCACTCTGGG ACTGGGACCC CAACACCAGC CTTCTGGAAC AGGGCCACAT 
TGTCCATTAC CAGTTGCAGG TCCTCATCTC CAGCCCCAAG GACCAAACAG TATTCCAACA 
CCTACCGCTT CAGGGTTCTG TCCTCACCCT GGCTCTGTGG CCCTGCCACA TGGGGTCCAA 
GGACCTCAGC AGGCATCTCC AGTGCCTGGA CAGATTCCAA TTCACAGAGC ACAGGTGCCA 
CCGACATTTC AGAACAATTA CCATGGTTCG GGGTGGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205424.1
CDS	253..5394
Translation	MGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSGAESPCVPCSPNHVPSSPSSHSNHIPQLQPKGPVPSFSELTEDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_005205367.4
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205367.4

ORF Insert Sequence:

ATGGGGATTG ACAGGCAGCA TATTCCTGAT ACATATCTTT GTGAACGTTG TCAGCCTAGG 
AGTTTGGATA AAGAGAGGGC GGTGCTACTA CAACGCCGGA AAAGGGAAAA TATGTCAGAT 
GGTGATACCA GTGCAACTGA GAGTGGTGAT GAGGTTCCTG TGGAATTATA TACTGCATTT 
CAGCATACCC CAACATCGAT TACTTTAACT GCTTCAAGGG TTTCTAAGGT TAATGATAAA 
AGAAGGAAAA AAAGTGGGGA GAAAGAACAA AATATTTCAA AATGTAAAAA AGCCTTTCGT 
GAAGGATCTA GGAAATCATC AAGAGTTAAG GGCTCAGCTC CGGAGATTGA TCCTTCATCT 
GATGTTTCAA ATTTTGGATG GGAGACAAAA ATCAAAGCAT GGATGGATCA ATATGAAGAG 
GCAAATAATA ACCAATATAG TGAGAGTGTT CAGAGGGAGG CACAAAGAAT AGCTCTGAGA 
TTGGGCAATG GAAATGACAA AAAAGAGATT AAATCAGATT TGAATACCAA CAATTTGATC 
TTCAAACCTC CTGTAGAGAG CCATATACAA AAAAATAAGA AGATTCTGAA ATCTGCAAAA 
GATTTGCCTC CTGATGCACT TATCATTGAA TACAGAGGGA AGTTTATGTT GAGAGAGCAG 
TTTGAAGCAA ATGGATATTT CTTTAAGAGA CCATACCCTT TTGTTTTATT CTATTCTAAA 
TTTCATGGCC TAGAAATGTG TGTTGATGCA AGGACTTTTG GGAATGAAGC TCGGTTCATC 
AGGCGTTCTT GTACACCCAA TGCAGAGGTG AGGCATGAAA TTGAAGATGG AACCATACAT 
CTTTATATTT ATTCTATACA AAGTATTCCA AAGGGAACCG AAATTACTAT TGCCTTTGAT 
TTTGACTATG GGAATTGTAA GTACAAGGTG GATTGTGCAT GCCTCAAAGA AAATTCAGAG 
TGCCCTGTTC TGAAACGTAG TTCTGAATCC ACGGAGAACA TCAATAGCGG TTATGAGACC 
AGAAGGAAGA AAGGCAAAAA AGAAAAGGAT ATTTCAAAAG AAAAAGACAC ACAAAATCAG 
AATATTACTC TGGATTGTGA AGGAACAACG AACAAAATGA AGAGCCCAGA AACTAAACAG 
AGAAAGCTTT CTCCACTGAG ACTATCAGTA TCAAATAATC AGGAACCAGA TTTTATTGAT 
GATATAGAAG AAAAAACTCC CATCAGCAAT GAGGTAGAAA TGGAGTCTGA GGAACAGATT 
GCAGAGAGGA AAAGGAAGAT GACAAGAGAA GAAAGGAAAA TGGAAGCAAT TCTGCAGGCT 
TTTGCCAGAC TTGAAAAAAG AGAGAAGAGA AGAGAACAAG CTTTGGAAAG AATCAGCACA 
GCCAAAACTG AAGTTAAAAC TGAATGTAAA GATGCACAGA CTGTCAGTGA TGCTGAAGTT 
ATTCAGGAAC AAGCAAAAGA AGAAACTGCT AGCAAGCCAA CCCCAGCCAA AGTAAATAGA 
ACTAAACAGA GAAAAAGTTT TTCTCGGAGC AGGACTCACA TTGGACAGCA GCGTCGGAGA 
CACAGGACTG TCAGTATGTG TTCGGATATC CAACCGTCTT CTCCTGACAT CGAAGTTACC 
TCACAACAAA ACGATGTGGA AAATACTGTA CTTGCAATAG AACCAGAAAC TGAAACTGCA 
CTAACAGAAA TAATTACTGA AAATGAAGTT CCAGTACTTA ATAAATGTCC AACAAAGTAC 
CCTAAAACAA AGAAGCACTT GGTCAGTGAA TGGTTAAGTG AGAAGAATGA GAAGACAGGA 
AAACCTTCAG ACAGCCTTTC AGAGAGGCCG CTGCGCATAA CTACAGATCC TGAAGTGCTA 
GCTACACAGC TCAATTCTTT GCCAGGTCTC ACTTACAGTC CACATGTATA CTCTACTCCT 
AAGCACTATA TTAGATTCAC TTCACCATTC CTTTCGGAAA AAAGGAGAAG AAAAGAACCT 
CCTGAAAACA TATCTGGCTC ATGCAAGAAG CGGTGGTTGA AACAAGCTCT GGAAGAGGAA 
AACTCAGCAA TTTTACATAG ATTTAATTCG CCCTGTCAAG AAAGATCCAG AAGTCCTACA 
GTCAATGGTG AAAATAAAAG TCCACTACTA TTAAATGATA GCTGTTCCCT TCCAGATTTA 
ACTACACCAC TGAAAAAACG AAGACTTTAT CAGTTGCTAG ATTCTGCTTA CTCAGAAACC 
TCCACACCTA CTCCTTCACC GTATGCTACA CCAACTCACA CAGATATTAC TCCTACAGAC 
CCATCATTTT CCACTCCTCC ACGGATAAAG TCAGATGATG AAGCTTGTAG AAATGGTTAT 
AAGCCCATAT ATTCACCAGT TACCCCAGTA ACTCCTGGCA CACCAGGAAA TACCATGCAC 
TTTGAGAATA TTTCTTCCCC AGAAAGTTCT CCAGAAATTA AGAGACGCAC TTATAGTCAA 
GAGGGATATG ACAGATCGTC AACCATGTTA ACGTTGGGGC CTTTTAGAAA TTCTAACTTA 
ACTGAACTGG GTCTGCAAGA AATAAAGACT ATTGGCTATG GCAGCCCTAG GAGTAGGACT 
GAAATCAGCA GGCAGTGCCC TGGAGAAAAG GAACCGGTGT CAGATCTTCA ACTGGGACTC 
GATGCAGTCG AGCAGACCGC CTTACATAAA ACCATGGAAG CAGCTGCACA CGACAGGACT 
GAGGCTGCCA GCCAGCTGGA GGCTGCTCAC TCGGGGCGGG GCCCTCTGTA TTCCTCCTGG 
GTAAAGAGTC CTGAGAGAAC AGGCGTTAAC TTCTCAGTAA ATTCCAACTT GAGGGACCTG 
ACACCCTCAC ATCAGTTGGA GGTTGGAGGC GGCTTCCGAA TAAGTGAGTC AAAGTGCCTG 
ATGCAGGATG ATACTAGAGG CATGTTTATG GAAACACCCG TGTTTTGTAC TTCAGAAGAT 
GGGCTTGTAT CTGGTTTCGG ACGGACTGTT AATGACAATT TGATCGACGG AAGTTGCACA 
CCCCAGAATC CACCACAAAA GAAAAAGGTT TCTCTATTAG AATACCGGAA GAGACAACGT 
GAAGCTAGGA AGAGCGGCTC TAAGACGGAG CACTTTGCGC TGGTTAGTGT ATCACCTCAC 
ACAAGTGGCA ACTTGAGCAG CAGCACTGGT GATGGGTGTG CCCACAGGAG TGAAAATGGG 
GAGCAGGTAG AAAGCGCTGC TAGCCTACCT TTACCAACAC CAGCTACAGT TTATCATGCT 
ACTTCGGAAG AAACCAGCAA TAACAGTACT GTTAAGGAAG TGTCTGCCAG TGAAAAGAAT 
GAACCAGAAG TTCAATGGAC GGCCTCAACT TCAGTGGAGC AAGTGAGAGA GAGGAGTTAT 
CAGAGAGCTC TGCTTCTCAG TGATCACCGA AAAGATAAAG ACAGTGGGGC AGAGTCACCA 
TGTGTCCCCT GTTCACCGAA TCATGTTCCG TCTTCTCCTT CATCTCATTC AAATCACATT 
CCCCAGTTGC AACCCAAGGG CCCAGTCCCT TCTTTCAGTG AACTTACGGA AGATCCTGAT 
CCTGAAAATC TAGAACCCAC AACTACAAAT GAATGCCCAT CCCCAGATAC TTCCCAAAGT 
ACTTGTAAAA GTCCTTCAAA AATGAGCAAG CCTGGTTCAC CTGGACCTGT AATTCCTGTT 
CAGCCACATG GGAAAATACT TACAAAACCA GATTCCCATT GGGAGGGAAC AGTTATTGTA 
TCTGAAGCTG AGAATGGTGT CCACCTGAAA ACAGAACTCC ATCAAAAACA GCTGTCAAGT 
AACCCCCAAG CACTTTCAAA GAACCATCCT CCACAGCCAC TCGTTCGCTG TTCATCTGAG 
CAGCTTTCAC AAAAGCTGCC TTCTGCACCC ATGAAGTTGC ACTGTCCTCC GTCACCTCAC 
GTAGAAAATC CTCCAAAGTC ATCGACGCCT CACACGCCTG TACAGCATGG CTATCTCTCA 
CCAAAGCCGC CTACACAGCA GTTAGGCTCT CCCTATAGGC CTCACCATTC ACAGTCACCT 
CAAGTTGGAA CACCTCAGCG AGAGCCTCAA AGAAATTTTT ACCCAGCCGC ACAGAACCTT 
CAAGCCAGTA CTCAGCAGGC AACTTCTGGA GCATTATTTA CACAGACACC CTCAGGACAA 
TCTTCAGCAA CATACAGTCA GTTTAATCAG ACAAGTCTGA GCAGCACGGC ACCACCCCCT 
CCACCCCCTC CACCCCCTTC TTCTTCGTCT TATTATCAAA ACCAGCAGCC CTCTGCAAAC 
TTTCAGAATT ATAATCAGCT TAAAGGTAGT CTTTCCCAAC AAACTGTGTT TACATCAGGA 
CCAAATCAAG CACTTCCTGG CACCACAAGC CAGCAAACAG TTCCAGGACA CCACGTTACT 
CCAGGGCATT TTTTGCCTTC TCAGAACCCT ACTGTTCACC ATCAGACTGC TGCTGCTGTG 
GTCCCACCCC CTCCTCCACC CCCACCTGCT CCAGGACCAC ACCTCGTACA ACAGCCAAGC 
TCCCATCAGC CACACTCAGT AGCACATGTG GTGGGGCCTG TTCACGCTGT CACCCCTGGA 
TCGCATATTC ATTCTCAAAC TGCTGGGCAC CATTTGCCAC CACCGCCTCC CCCTCCTGGT 
CCTGCCCCTC ATCACCACCC CCCGCCCCAC CCTTCCACAG GACTCCAAGG TCTACAAGCA 
CAACACCAGC ATGTGGTAAA CTCAGCCCCT CCACCTCCCC CTCCTCCTCC GCCTTCCAGT 
GTTCTGGCTT CTGGGCATCA CACCACGTCA GCTCAAGCCT TACACCATCC ACCTCATCAA 
GGACCGCCAC TTTTTCCTTC AAGTGCTCAT CCAGCTGTAC CACCGTATCC CTCACAAGCT 
ACACATCACA CCACTCTGGG ACTGGGACCC CAACACCAGC CTTCTGGAAC AGGGCCACAT 
TGTCCATTAC CAGTTGCAGG TCCTCATCTC CAGCCCCAAG GACCAAACAG TATTCCAACA 
CCTACCGCTT CAGGGTTCTG TCCTCACCCT GGCTCTGTGG CCCTGCCACA TGGGGTCCAA 
GGACCTCAGC AGGCATCTCC AGTGCCTGGA CAGATTCCAA TTCACAGAGC ACAGGTGCCA 
CCGACATTTC AGAACAATTA CCATGGTTCG GGGTGGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KMT2E ( XM_024990819.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846587.1 Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa233779
Clone ID Related Accession (Same CDS sequence)	XM_024990819.1
Accession Version	XM_024990819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive histone-lysine N-methyltransferase 2E isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGATTG ACAGGCAGCA TATTCCTGAT ACATATCTTT GTGAACGTTG TCAGCCTAGG 
AGTTTGGATA AAGAGAGGGC GGTGCTACTA CAACGCCGGA AAAGGGAAAA TATGTCAGAT 
GGTGATACCA GTGCAACTGA GAGTGGTGAT GAGGTTCCTG TGGAATTATA TACTGCATTT 
CAGCATACCC CAACATCGAT TACTTTAACT GCTTCAAGGG TTTCTAAGGT TAATGATAAA 
AGAAGGAAAA AAAGTGGGGA GAAAGAACAA AATATTTCAA AATGTAAAAA AGCCTTTCGT 
GAAGGATCTA GGAAATCATC AAGAGTTAAG GGCTCAGCTC CGGAGATTGA TCCTTCATCT 
GATGTTTCAA ATTTTGGATG GGAGACAAAA ATCAAAGCAT GGATGGATCA ATATGAAGAG 
GCAAATAATA ACCAATATAG TGAGAGTGTT CAGAGGGAGG CACAAAGAAT AGCTCTGAGA 
TTGGGCAATG GAAATGACAA AAAAGAGATT AAATCAGATT TGAATACCAA CAATTTGATC 
TTCAAACCTC CTGTAGAGAG CCATATACAA AAAAATAAGA AGATTCTGAA ATCTGCAAAA 
GATTTGCCTC CTGATGCACT TATCATTGAA TACAGAGGGA AGTTTATGTT GAGAGAGCAG 
TTTGAAGCAA ATGGATATTT CTTTAAGAGA CCATACCCTT TTGTTTTATT CTATTCTAAA 
TTTCATGGCC TAGAAATGTG TGTTGATGCA AGGACTTTTG GGAATGAAGC TCGGTTCATC 
AGGCGTTCTT GTACACCCAA TGCAGAGGTG AGGCATGAAA TTGAAGATGG AACCATACAT 
CTTTATATTT ATTCTATACA AAGTATTCCA AAGGGAACCG AAATTACTAT TGCCTTTGAT 
TTTGACTATG GGAATTGTAA GTACAAGGTG GATTGTGCAT GCCTCAAAGA AAATTCAGAG 
TGCCCTGTTC TGAAACGTAG TTCTGAATCC ACGGAGAACA TCAATAGCGG TTATGAGACC 
AGAAGGAAGA AAGGCAAAAA AGAAAAGGAT ATTTCAAAAG AAAAAGACAC ACAAAATCAG 
AATATTACTC TGGATTGTGA AGGAACAACG AACAAAATGA AGAGCCCAGA AACTAAACAG 
AGAAAGCTTT CTCCACTGAG ACTATCAGTA TCAAATAATC AGGAACCAGA TTTTATTGAT 
GATATAGAAG AAAAAACTCC CATCAGCAAT GAGGTAGAAA TGGAGTCTGA GGAACAGATT 
GCAGAGAGGA AAAGGAAGAT GACAAGAGAA GAAAGGAAAA TGGAAGCAAT TCTGCAGGCT 
TTTGCCAGAC TTGAAAAAAG AGAGAAGAGA AGAGAACAAG CTTTGGAAAG AATCAGCACA 
GCCAAAACTG AAGTTAAAAC TGAATGTAAA GATGCACAGA CTGTCAGTGA TGCTGAAGTT 
ATTCAGGAAC AAGCAAAAGA AGAAACTGCT AGCAAGCCAA CCCCAGCCAA AGTAAATAGA 
ACTAAACAGA GAAAAAGTTT TTCTCGGAGC AGGACTCACA TTGGACAGCA GCGTCGGAGA 
CACAGGACTG TCAGTATGTG TTCGGATATC CAACCGTCTT CTCCTGACAT CGAAGTTACC 
TCACAACAAA ACGATGTGGA AAATACTGTA CTTGCAATAG AACCAGAAAC TGAAACTGCA 
CTAACAGAAA TAATTACTGA AAATGAAGTT CCAGTACTTA ATAAATGTCC AACAAAGTAC 
CCTAAAACAA AGAAGCACTT GGTCAGTGAA TGGTTAAGTG AGAAGAATGA GAAGACAGGA 
AAACCTTCAG ACAGCCTTTC AGAGAGGCCG CTGCGCATAA CTACAGATCC TGAAGTGCTA 
GCTACACAGC TCAATTCTTT GCCAGGTCTC ACTTACAGTC CACATGTATA CTCTACTCCT 
AAGCACTATA TTAGATTCAC TTCACCATTC CTTTCGGAAA AAAGGAGAAG AAAAGAACCT 
CCTGAAAACA TATCTGGCTC ATGCAAGAAG CGGTGGTTGA AACAAGCTCT GGAAGAGGAA 
AACTCAGCAA TTTTACATAG ATTTAATTCG CCCTGTCAAG AAAGATCCAG AAGTCCTACA 
GTCAATGGTG AAAATAAAAG TCCACTACTA TTAAATGATA GCTGTTCCCT TCCAGATTTA 
ACTACACCAC TGAAAAAACG AAGACTTTAT CAGTTGCTAG ATTCTGCTTA CTCAGAAACC 
TCCACACCTA CTCCTTCACC GTATGCTACA CCAACTCACA CAGATATTAC TCCTACAGAC 
CCATCATTTT CCACTCCTCC ACGGATAAAG TCAGATGATG AAGCTTGTAG AAATGGTTAT 
AAGCCCATAT ATTCACCAGT TACCCCAGTA ACTCCTGGCA CACCAGGAAA TACCATGCAC 
TTTGAGAATA TTTCTTCCCC AGAAAGTTCT CCAGAAATTA AGAGACGCAC TTATAGTCAA 
GAGGGATATG ACAGATCGTC AACCATGTTA ACGTTGGGGC CTTTTAGAAA TTCTAACTTA 
ACTGAACTGG GTCTGCAAGA AATAAAGACT ATTGGCTATG GCAGCCCTAG GAGTAGGACT 
GAAATCAGCA GGCAGTGCCC TGGAGAAAAG GAACCGGTGT CAGATCTTCA ACTGGGACTC 
GATGCAGTCG AGCAGACCGC CTTACATAAA ACCATGGAAG CAGCTGCACA CGACAGGACT 
GAGGCTGCCA GCCAGCTGGA GGCTGCTCAC TCGGGGCGGG GCCCTCTGTA TTCCTCCTGG 
GTAAAGAGTC CTGAGAGAAC AGGCGTTAAC TTCTCAGTAA ATTCCAACTT GAGGGACCTG 
ACACCCTCAC ATCAGTTGGA GGTTGGAGGC GGCTTCCGAA TAAGTGAGTC AAAGTGCCTG 
ATGCAGGATG ATACTAGAGG CATGTTTATG GAAACACCCG TGTTTTGTAC TTCAGAAGAT 
GGGCTTGTAT CTGGTTTCGG ACGGACTGTT AATGACAATT TGATCGACGG AAGTTGCACA 
CCCCAGAATC CACCACAAAA GAAAAAGGTT TCTCTATTAG AATACCGGAA GAGACAACGT 
GAAGCTAGGA AGAGCGGCTC TAAGACGGAG CACTTTGCGC TGGTTAGTGT ATCACCTCAC 
ACAAGTGGCA ACTTGAGCAG CAGCACTGGT GATGGGTGTG CCCACAGGAG TGAAAATGGG 
GAGCAGGTAG AAAGCGCTGC TAGCCTACCT TTACCAACAC CAGCTACAGT TTATCATGCT 
ACTTCGGAAG AAACCAGCAA TAACAGTACT GTTAAGGAAG TGTCTGCCAG TGAAAAGAAT 
GAACCAGAAG TTCAATGGAC GGCCTCAACT TCAGTGGAGC AAGTGAGAGA GAGGAGTTAT 
CAGAGAGCTC TGCTTCTCAG TGATCACCGA AAAGATAAAG ACAGTGATCC TGATCCTGAA 
AATCTAGAAC CCACAACTAC AAATGAATGC CCATCCCCAG ATACTTCCCA AAGTACTTGT 
AAAAGTCCTT CAAAAATGAG CAAGCCTGGT TCACCTGGAC CTGTAATTCC TGTTCAGCCA 
CATGGGAAAA TACTTACAAA ACCAGATTCC CATTGGGAGG GAACAGTTAT TGTATCTGAA 
GCTGAGAATG GTGTCCACCT GAAAACAGAA CTCCATCAAA AACAGCTGTC AAGTAACCCC 
CAAGCACTTT CAAAGAACCA TCCTCCACAG CCACTCGTTC GCTGTTCATC TGAGCAGCTT 
TCACAAAAGC TGCCTTCTGC ACCCATGAAG TTGCACTGTC CTCCGTCACC TCACGTAGAA 
AATCCTCCAA AGTCATCGAC GCCTCACACG CCTGTACAGC ATGGCTATCT CTCACCAAAG 
CCGCCTACAC AGCAGTTAGG CTCTCCCTAT AGGCCTCACC ATTCACAGTC ACCTCAAGTT 
GGAACACCTC AGCGAGAGCC TCAAAGAAAT TTTTACCCAG CCGCACAGAA CCTTCAAGCC 
AGTACTCAGC AGGCAACTTC TGGAGCATTA TTTACACAGA CACCCTCAGG ACAATCTTCA 
GCAACATACA GTCAGTTTAA TCAGACAAGT CTGAGCAGCA CGGCACCACC CCCTCCACCC 
CCTCCACCCC CTTCTTCTTC GTCTTATTAT CAAAACCAGC AGCCCTCTGC AAACTTTCAG 
AATTATAATC AGCTTAAAGG TAGTCTTTCC CAACAAACTG TGTTTACATC AGGACCAAAT 
CAAGCACTTC CTGGCACCAC AAGCCAGCAA ACAGTTCCAG GACACCACGT TACTCCAGGG 
CATTTTTTGC CTTCTCAGAA CCCTACTGTT CACCATCAGA CTGCTGCTGC TGTGGTCCCA 
CCCCCTCCTC CACCCCCACC TGCTCCAGGA CCACACCTCG TACAACAGCC AAGCTCCCAT 
CAGCCACACT CAGTAGCACA TGTGGTGGGG CCTGTTCACG CTGTCACCCC TGGATCGCAT 
ATTCATTCTC AAACTGCTGG GCACCATTTG CCACCACCGC CTCCCCCTCC TGGTCCTGCC 
CCTCATCACC ACCCCCCGCC CCACCCTTCC ACAGGACTCC AAGGTCTACA AGCACAACAC 
CAGCATGTGG TAAACTCAGC CCCTCCACCT CCCCCTCCTC CTCCGCCTTC CAGTGTTCTG 
GCTTCTGGGC ATCACACCAC GTCAGCTCAA GCCTTACACC ATCCACCTCA TCAAGGACCG 
CCACTTTTTC CTTCAAGTGC TCATCCAGCT GTACCACCGT ATCCCTCACA AGCTACACAT 
CACACCACTC TGGGACTGGG ACCCCAACAC CAGCCTTCTG GAACAGGGCC ACATTGTCCA 
TTACCAGTTG CAGGTCCTCA TCTCCAGCCC CAAGGACCAA ACAGTATTCC AACACCTACC 
GCTTCAGGGT TCTGTCCTCA CCCTGGCTCT GTGGCCCTGC CACATGGGGT CCAAGGACCT 
CAGCAGGCAT CTCCAGTGCC TGGACAGATT CCAATTCACA GAGCACAGGT GCCACCGACA 
TTTCAGAACA ATTACCATGG TTCGGGGTGG CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846587.1
CDS	998..6013
Translation	MGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_024990819.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990819.1

ORF Insert Sequence:

ATGGGGATTG ACAGGCAGCA TATTCCTGAT ACATATCTTT GTGAACGTTG TCAGCCTAGG 
AGTTTGGATA AAGAGAGGGC GGTGCTACTA CAACGCCGGA AAAGGGAAAA TATGTCAGAT 
GGTGATACCA GTGCAACTGA GAGTGGTGAT GAGGTTCCTG TGGAATTATA TACTGCATTT 
CAGCATACCC CAACATCGAT TACTTTAACT GCTTCAAGGG TTTCTAAGGT TAATGATAAA 
AGAAGGAAAA AAAGTGGGGA GAAAGAACAA AATATTTCAA AATGTAAAAA AGCCTTTCGT 
GAAGGATCTA GGAAATCATC AAGAGTTAAG GGCTCAGCTC CGGAGATTGA TCCTTCATCT 
GATGTTTCAA ATTTTGGATG GGAGACAAAA ATCAAAGCAT GGATGGATCA ATATGAAGAG 
GCAAATAATA ACCAATATAG TGAGAGTGTT CAGAGGGAGG CACAAAGAAT AGCTCTGAGA 
TTGGGCAATG GAAATGACAA AAAAGAGATT AAATCAGATT TGAATACCAA CAATTTGATC 
TTCAAACCTC CTGTAGAGAG CCATATACAA AAAAATAAGA AGATTCTGAA ATCTGCAAAA 
GATTTGCCTC CTGATGCACT TATCATTGAA TACAGAGGGA AGTTTATGTT GAGAGAGCAG 
TTTGAAGCAA ATGGATATTT CTTTAAGAGA CCATACCCTT TTGTTTTATT CTATTCTAAA 
TTTCATGGCC TAGAAATGTG TGTTGATGCA AGGACTTTTG GGAATGAAGC TCGGTTCATC 
AGGCGTTCTT GTACACCCAA TGCAGAGGTG AGGCATGAAA TTGAAGATGG AACCATACAT 
CTTTATATTT ATTCTATACA AAGTATTCCA AAGGGAACCG AAATTACTAT TGCCTTTGAT 
TTTGACTATG GGAATTGTAA GTACAAGGTG GATTGTGCAT GCCTCAAAGA AAATTCAGAG 
TGCCCTGTTC TGAAACGTAG TTCTGAATCC ACGGAGAACA TCAATAGCGG TTATGAGACC 
AGAAGGAAGA AAGGCAAAAA AGAAAAGGAT ATTTCAAAAG AAAAAGACAC ACAAAATCAG 
AATATTACTC TGGATTGTGA AGGAACAACG AACAAAATGA AGAGCCCAGA AACTAAACAG 
AGAAAGCTTT CTCCACTGAG ACTATCAGTA TCAAATAATC AGGAACCAGA TTTTATTGAT 
GATATAGAAG AAAAAACTCC CATCAGCAAT GAGGTAGAAA TGGAGTCTGA GGAACAGATT 
GCAGAGAGGA AAAGGAAGAT GACAAGAGAA GAAAGGAAAA TGGAAGCAAT TCTGCAGGCT 
TTTGCCAGAC TTGAAAAAAG AGAGAAGAGA AGAGAACAAG CTTTGGAAAG AATCAGCACA 
GCCAAAACTG AAGTTAAAAC TGAATGTAAA GATGCACAGA CTGTCAGTGA TGCTGAAGTT 
ATTCAGGAAC AAGCAAAAGA AGAAACTGCT AGCAAGCCAA CCCCAGCCAA AGTAAATAGA 
ACTAAACAGA GAAAAAGTTT TTCTCGGAGC AGGACTCACA TTGGACAGCA GCGTCGGAGA 
CACAGGACTG TCAGTATGTG TTCGGATATC CAACCGTCTT CTCCTGACAT CGAAGTTACC 
TCACAACAAA ACGATGTGGA AAATACTGTA CTTGCAATAG AACCAGAAAC TGAAACTGCA 
CTAACAGAAA TAATTACTGA AAATGAAGTT CCAGTACTTA ATAAATGTCC AACAAAGTAC 
CCTAAAACAA AGAAGCACTT GGTCAGTGAA TGGTTAAGTG AGAAGAATGA GAAGACAGGA 
AAACCTTCAG ACAGCCTTTC AGAGAGGCCG CTGCGCATAA CTACAGATCC TGAAGTGCTA 
GCTACACAGC TCAATTCTTT GCCAGGTCTC ACTTACAGTC CACATGTATA CTCTACTCCT 
AAGCACTATA TTAGATTCAC TTCACCATTC CTTTCGGAAA AAAGGAGAAG AAAAGAACCT 
CCTGAAAACA TATCTGGCTC ATGCAAGAAG CGGTGGTTGA AACAAGCTCT GGAAGAGGAA 
AACTCAGCAA TTTTACATAG ATTTAATTCG CCCTGTCAAG AAAGATCCAG AAGTCCTACA 
GTCAATGGTG AAAATAAAAG TCCACTACTA TTAAATGATA GCTGTTCCCT TCCAGATTTA 
ACTACACCAC TGAAAAAACG AAGACTTTAT CAGTTGCTAG ATTCTGCTTA CTCAGAAACC 
TCCACACCTA CTCCTTCACC GTATGCTACA CCAACTCACA CAGATATTAC TCCTACAGAC 
CCATCATTTT CCACTCCTCC ACGGATAAAG TCAGATGATG AAGCTTGTAG AAATGGTTAT 
AAGCCCATAT ATTCACCAGT TACCCCAGTA ACTCCTGGCA CACCAGGAAA TACCATGCAC 
TTTGAGAATA TTTCTTCCCC AGAAAGTTCT CCAGAAATTA AGAGACGCAC TTATAGTCAA 
GAGGGATATG ACAGATCGTC AACCATGTTA ACGTTGGGGC CTTTTAGAAA TTCTAACTTA 
ACTGAACTGG GTCTGCAAGA AATAAAGACT ATTGGCTATG GCAGCCCTAG GAGTAGGACT 
GAAATCAGCA GGCAGTGCCC TGGAGAAAAG GAACCGGTGT CAGATCTTCA ACTGGGACTC 
GATGCAGTCG AGCAGACCGC CTTACATAAA ACCATGGAAG CAGCTGCACA CGACAGGACT 
GAGGCTGCCA GCCAGCTGGA GGCTGCTCAC TCGGGGCGGG GCCCTCTGTA TTCCTCCTGG 
GTAAAGAGTC CTGAGAGAAC AGGCGTTAAC TTCTCAGTAA ATTCCAACTT GAGGGACCTG 
ACACCCTCAC ATCAGTTGGA GGTTGGAGGC GGCTTCCGAA TAAGTGAGTC AAAGTGCCTG 
ATGCAGGATG ATACTAGAGG CATGTTTATG GAAACACCCG TGTTTTGTAC TTCAGAAGAT 
GGGCTTGTAT CTGGTTTCGG ACGGACTGTT AATGACAATT TGATCGACGG AAGTTGCACA 
CCCCAGAATC CACCACAAAA GAAAAAGGTT TCTCTATTAG AATACCGGAA GAGACAACGT 
GAAGCTAGGA AGAGCGGCTC TAAGACGGAG CACTTTGCGC TGGTTAGTGT ATCACCTCAC 
ACAAGTGGCA ACTTGAGCAG CAGCACTGGT GATGGGTGTG CCCACAGGAG TGAAAATGGG 
GAGCAGGTAG AAAGCGCTGC TAGCCTACCT TTACCAACAC CAGCTACAGT TTATCATGCT 
ACTTCGGAAG AAACCAGCAA TAACAGTACT GTTAAGGAAG TGTCTGCCAG TGAAAAGAAT 
GAACCAGAAG TTCAATGGAC GGCCTCAACT TCAGTGGAGC AAGTGAGAGA GAGGAGTTAT 
CAGAGAGCTC TGCTTCTCAG TGATCACCGA AAAGATAAAG ACAGTGATCC TGATCCTGAA 
AATCTAGAAC CCACAACTAC AAATGAATGC CCATCCCCAG ATACTTCCCA AAGTACTTGT 
AAAAGTCCTT CAAAAATGAG CAAGCCTGGT TCACCTGGAC CTGTAATTCC TGTTCAGCCA 
CATGGGAAAA TACTTACAAA ACCAGATTCC CATTGGGAGG GAACAGTTAT TGTATCTGAA 
GCTGAGAATG GTGTCCACCT GAAAACAGAA CTCCATCAAA AACAGCTGTC AAGTAACCCC 
CAAGCACTTT CAAAGAACCA TCCTCCACAG CCACTCGTTC GCTGTTCATC TGAGCAGCTT 
TCACAAAAGC TGCCTTCTGC ACCCATGAAG TTGCACTGTC CTCCGTCACC TCACGTAGAA 
AATCCTCCAA AGTCATCGAC GCCTCACACG CCTGTACAGC ATGGCTATCT CTCACCAAAG 
CCGCCTACAC AGCAGTTAGG CTCTCCCTAT AGGCCTCACC ATTCACAGTC ACCTCAAGTT 
GGAACACCTC AGCGAGAGCC TCAAAGAAAT TTTTACCCAG CCGCACAGAA CCTTCAAGCC 
AGTACTCAGC AGGCAACTTC TGGAGCATTA TTTACACAGA CACCCTCAGG ACAATCTTCA 
GCAACATACA GTCAGTTTAA TCAGACAAGT CTGAGCAGCA CGGCACCACC CCCTCCACCC 
CCTCCACCCC CTTCTTCTTC GTCTTATTAT CAAAACCAGC AGCCCTCTGC AAACTTTCAG 
AATTATAATC AGCTTAAAGG TAGTCTTTCC CAACAAACTG TGTTTACATC AGGACCAAAT 
CAAGCACTTC CTGGCACCAC AAGCCAGCAA ACAGTTCCAG GACACCACGT TACTCCAGGG 
CATTTTTTGC CTTCTCAGAA CCCTACTGTT CACCATCAGA CTGCTGCTGC TGTGGTCCCA 
CCCCCTCCTC CACCCCCACC TGCTCCAGGA CCACACCTCG TACAACAGCC AAGCTCCCAT 
CAGCCACACT CAGTAGCACA TGTGGTGGGG CCTGTTCACG CTGTCACCCC TGGATCGCAT 
ATTCATTCTC AAACTGCTGG GCACCATTTG CCACCACCGC CTCCCCCTCC TGGTCCTGCC 
CCTCATCACC ACCCCCCGCC CCACCCTTCC ACAGGACTCC AAGGTCTACA AGCACAACAC 
CAGCATGTGG TAAACTCAGC CCCTCCACCT CCCCCTCCTC CTCCGCCTTC CAGTGTTCTG 
GCTTCTGGGC ATCACACCAC GTCAGCTCAA GCCTTACACC ATCCACCTCA TCAAGGACCG 
CCACTTTTTC CTTCAAGTGC TCATCCAGCT GTACCACCGT ATCCCTCACA AGCTACACAT 
CACACCACTC TGGGACTGGG ACCCCAACAC CAGCCTTCTG GAACAGGGCC ACATTGTCCA 
TTACCAGTTG CAGGTCCTCA TCTCCAGCCC CAAGGACCAA ACAGTATTCC AACACCTACC 
GCTTCAGGGT TCTGTCCTCA CCCTGGCTCT GTGGCCCTGC CACATGGGGT CCAAGGACCT 
CAGCAGGCAT CTCCAGTGCC TGGACAGATT CCAATTCACA GAGCACAGGT GCCACCGACA 
TTTCAGAACA ATTACCATGG TTCGGGGTGG CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KMT2E ( NM_001082451.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001075920.2 Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), mRNA.

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CloneID	OBa01901
Clone ID Related Accession (Same CDS sequence)	NM_001082451.2 , XM_005205363.4 , XM_005205365.4 , XM_005205362.4 , NM_001082451.3 , XM_005205364.4
Accession Version	NM_001082451.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-31
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase 2E
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03120932.1. On Dec 23, 2009 this sequence version replaced NM_001082451.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075920.2
CDS	381..5960
Translation	MSIVIPLGVDTAETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSVLISKNEVSIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSGAESPCVPCSPNHVPSSPSSHSNHIPQLQPKGPVPSFSELTEDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	NM_001082451.2
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082451.2

ORF Insert Sequence:

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KMT2E ( NM_001082451.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001075920.2 Bos taurus lysine methyltransferase 2E (KMT2E), mRNA.

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CloneID	OBa01901
Clone ID Related Accession (Same CDS sequence)	NM_001082451.2 , XM_005205363.4 , XM_005205365.4 , XM_005205362.4 , NM_001082451.3 , XM_005205364.4
Accession Version	NM_001082451.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-15
Organism	Bos taurus(cattle)
Product	inactive histone-lysine N-methyltransferase 2E
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000004.1. On Jun 16, 2018 this sequence version replaced NM_001082451.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR6764281.15648.1, SRR5571260.45118.1 [ECO:0000331] ##Evidence-Data-END##

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075920.2
CDS	381..5960
Translation	MSIVIPLGVDTAETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSVLISKNEVSIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSGAESPCVPCSPNHVPSSPSSHSNHIPQLQPKGPVPSFSELTEDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	NM_001082451.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine methyltransferase 2E (KMT2E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082451.3

ORF Insert Sequence:

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KMT2E ( XM_005205363.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205420.1 Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X2, mRNA.

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CloneID	OBa01901
Clone ID Related Accession (Same CDS sequence)	NM_001082451.2 , XM_005205363.4 , XM_005205365.4 , XM_005205362.4 , NM_001082451.3 , XM_005205364.4
Accession Version	XM_005205363.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205363.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205420.1
CDS	554..6133
Translation	MSIVIPLGVDTAETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSVLISKNEVSIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSGAESPCVPCSPNHVPSSPSSHSNHIPQLQPKGPVPSFSELTEDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_005205363.4
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205363.4

ORF Insert Sequence:

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KMT2E ( XM_005205365.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205422.1 Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X4, mRNA.

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CloneID	OBa01901
Clone ID Related Accession (Same CDS sequence)	NM_001082451.2 , XM_005205363.4 , XM_005205365.4 , XM_005205362.4 , NM_001082451.3 , XM_005205364.4
Accession Version	XM_005205365.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205365.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205422.1
CDS	176..5755
Translation	MSIVIPLGVDTAETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSVLISKNEVSIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSGAESPCVPCSPNHVPSSPSSHSNHIPQLQPKGPVPSFSELTEDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_005205365.4
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205365.4

ORF Insert Sequence:

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KMT2E ( XM_005205362.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205419.1 Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X1, mRNA.

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CloneID	OBa01901
Clone ID Related Accession (Same CDS sequence)	NM_001082451.2 , XM_005205363.4 , XM_005205365.4 , XM_005205362.4 , NM_001082451.3 , XM_005205364.4
Accession Version	XM_005205362.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205362.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205419.1
CDS	510..6089
Translation	MSIVIPLGVDTAETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSVLISKNEVSIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSGAESPCVPCSPNHVPSSPSSHSNHIPQLQPKGPVPSFSELTEDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_005205362.4
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205362.4

ORF Insert Sequence:

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KMT2E ( XM_005205364.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205421.1 Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X3, mRNA.

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CloneID	OBa01901
Clone ID Related Accession (Same CDS sequence)	NM_001082451.2 , XM_005205363.4 , XM_005205365.4 , XM_005205362.4 , NM_001082451.3 , XM_005205364.4
Accession Version	XM_005205364.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205364.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205421.1
CDS	613..6192
Translation	MSIVIPLGVDTAETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSVLISKNEVSIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSGAESPCVPCSPNHVPSSPSSHSNHIPQLQPKGPVPSFSELTEDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_005205364.4
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205364.4

ORF Insert Sequence:

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KMT2E ( XM_005205362.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205419.1 Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X1, mRNA.

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CloneID	OBa18519
Clone ID Related Accession (Same CDS sequence)	XM_005205362.3 , XM_005205363.3 , XM_005205364.3 , XM_005205365.3
Accession Version	XM_005205362.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205362.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205419.1
CDS	450..6029
Translation	MSIVIPLGVDTAETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSVLISKNEVSIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSGAESPCVPCSPNHVPSSPSSHSNHIPQLQPKGPVPSFSELTEDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_005205362.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205362.3

ORF Insert Sequence:

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KMT2E ( XM_005205363.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205420.1 Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X2, mRNA.

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CloneID	OBa18519
Clone ID Related Accession (Same CDS sequence)	XM_005205362.3 , XM_005205363.3 , XM_005205364.3 , XM_005205365.3
Accession Version	XM_005205363.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205363.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205420.1
CDS	523..6102
Translation	MSIVIPLGVDTAETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSVLISKNEVSIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSGAESPCVPCSPNHVPSSPSSHSNHIPQLQPKGPVPSFSELTEDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_005205363.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205363.3

ORF Insert Sequence:

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KMT2E ( XM_005205364.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205421.1 Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X3, mRNA.

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CloneID	OBa18519
Clone ID Related Accession (Same CDS sequence)	XM_005205362.3 , XM_005205363.3 , XM_005205364.3 , XM_005205365.3
Accession Version	XM_005205364.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205364.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205421.1
CDS	592..6171
Translation	MSIVIPLGVDTAETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSVLISKNEVSIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSGAESPCVPCSPNHVPSSPSSHSNHIPQLQPKGPVPSFSELTEDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_005205364.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205364.3

ORF Insert Sequence:

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KMT2E ( XM_005205365.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205422.1 Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X4, mRNA.

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CloneID	OBa18519
Clone ID Related Accession (Same CDS sequence)	XM_005205362.3 , XM_005205363.3 , XM_005205364.3 , XM_005205365.3
Accession Version	XM_005205365.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205365.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205422.1
CDS	144..5723
Translation	MSIVIPLGVDTAETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSVLISKNEVSIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSGAESPCVPCSPNHVPSSPSSHSNHIPQLQPKGPVPSFSELTEDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_005205365.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205365.3

ORF Insert Sequence:

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA 
TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA 
CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC 
CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT 
GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG 
GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT 
CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC 
GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC 
TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA 
CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT 
CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC 
CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA 
CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC 
AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC 
CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA 
ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT 
CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT 
CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC 
CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT 
CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA 
CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA 
CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT 
CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA 
CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA 
CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT 
TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA 
CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC 
CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT 
CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KMT2E ( XM_005205366.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205423.1 Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X5, mRNA.

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CloneID	OBa18520
Clone ID Related Accession (Same CDS sequence)	XM_005205366.3
Accession Version	XM_005205366.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase 2E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205366.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGATCCTG ATCCTGAAAA TCTAGAACCC ACAACTACAA ATGAATGCCC ATCCCCAGAT 
ACTTCCCAAA GTACTTGTAA AAGTCCTTCA AAAATGAGCA AGCCTGGTTC ACCTGGACCT 
GTAATTCCTG TTCAGCCACA TGGGAAAATA CTTACAAAAC CAGATTCCCA TTGGGAGGGA 
ACAGTTATTG TATCTGAAGC TGAGAATGGT GTCCACCTGA AAACAGAACT CCATCAAAAA 
CAGCTGTCAA GTAACCCCCA AGCACTTTCA AAGAACCATC CTCCACAGCC ACTCGTTCGC 
TGTTCATCTG AGCAGCTTTC ACAAAAGCTG CCTTCTGCAC CCATGAAGTT GCACTGTCCT 
CCGTCACCTC ACGTAGAAAA TCCTCCAAAG TCATCGACGC CTCACACGCC TGTACAGCAT 
GGCTATCTCT CACCAAAGCC TCCTACACAG CAGTTAGGCT CTCCCTATAG GCCTCACCAT 
TCACAGTCAC CTCAAGTTGG AACACCTCAG CGAGAGCCTC AAAGAAATTT TTACCCAGCC 
GCACAGAACC TTCAAGCCAG TACTCAGCAG GCAACTTCTG GAGCATTATT TACACAGACA 
CCCTCAGGAC AATCTTCAGC AACATACAGT CAGTTTAATC AGACAAGTCT GAGCAGCACG 
GCACCACCCC CTCCACCCCC TCCACCCCCT TCTTCTTCGT CTTATTATCA AAACCAGCAG 
CCCTCTGCAA ACTTTCAGAA TTATAATCAG CTTAAAGGTA GTCTTTCCCA ACAAACTGTG 
TTTACATCAG GACCAAATCA AGCACTTCCT GGCACCACAA GCCAGCAAAC AGTTCCAGGA 
CACCACGTTA CTCCAGGGCA TTTTTTGCCT TCTCAGAACC CTACTGTTCA CCATCAGACT 
GCTGCTGCTG TGGTCCCACC CCCTCCTCCA CCCCCACCTG CTCCAGGACC ACACCTCGTA 
CAACAGCCAA GCTCCCATCA GCCACACTCA GTAGCACATG TGGTGGGGCC TGTTCACGCT 
GTCACCCCTG GATCGCATAT TCATTCTCAA ACTGCTGGGC ACCATTTGCC ACCACCGCCT 
CCCCCTCCTG GTCCTGCCCC TCATCACCAC CCCCCGCCCC ACCCTTCCAC AGGACTCCAA 
GGTCTACAAG CACAACACCA GCATGTGGTA AACTCAGCCC CTCCACCTCC CCCTCCTCCT 
CCGCCTTCCA GTGTTCTGGC TTCTGGGCAT CACACCACGT CAGCTCAAGC CTTACACCAT 
CCACCTCATC AAGGACCGCC ACTTTTTCCT TCAAGTGCTC ATCCAGCTGT ACCACCGTAT 
CCCTCACAAG CTACACATCA CACCACTCTG GGACTGGGAC CCCAACACCA GCCTTCTGGA 
ACAGGGCCAC ATTGTCCATT ACCAGTTGCA GGTCCTCATC TCCAGCCCCA AGGACCAAAC 
AGTATTCCAA CACCTACCGC TTCAGGGTTC TGTCCTCACC CTGGCTCTGT GGCCCTGCCA 
CATGGGGTCC AAGGACCTCA GCAGGCATCT CCAGTGCCTG GACAGATTCC AATTCACAGA 
GCACAGGTGC CACCGACATT TCAGAACAAT TACCATGGTT CGGGGTGGCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205423.1
CDS	450..5903
Translation	MSIVIPLGVDTAETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSVLISKNEVSIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_005205366.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205366.3

ORF Insert Sequence:

ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT 
TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC 
TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA 
TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT 
TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG 
TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT 
TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT 
GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA 
AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG 
GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT 
TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA 
TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG 
GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG 
ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA 
CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG 
AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG 
ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG 
TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT 
GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG 
AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT 
GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA 
ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC 
CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC 
AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA 
AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG 
AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG 
GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG 
GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG 
GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT 
TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT 
GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC 
CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT 
GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT 
CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA 
CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT 
AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG 
AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT 
ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA 
CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA 
AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA 
CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA 
AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC 
TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT 
TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA 
GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA 
GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA 
CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG 
AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT 
TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC 
AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA 
GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA 
GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC 
CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT 
TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA 
AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG 
TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG 
ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA 
TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG 
GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC 
CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA 
GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG 
TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA 
GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC 
AGTGATCCTG ATCCTGAAAA TCTAGAACCC ACAACTACAA ATGAATGCCC ATCCCCAGAT 
ACTTCCCAAA GTACTTGTAA AAGTCCTTCA AAAATGAGCA AGCCTGGTTC ACCTGGACCT 
GTAATTCCTG TTCAGCCACA TGGGAAAATA CTTACAAAAC CAGATTCCCA TTGGGAGGGA 
ACAGTTATTG TATCTGAAGC TGAGAATGGT GTCCACCTGA AAACAGAACT CCATCAAAAA 
CAGCTGTCAA GTAACCCCCA AGCACTTTCA AAGAACCATC CTCCACAGCC ACTCGTTCGC 
TGTTCATCTG AGCAGCTTTC ACAAAAGCTG CCTTCTGCAC CCATGAAGTT GCACTGTCCT 
CCGTCACCTC ACGTAGAAAA TCCTCCAAAG TCATCGACGC CTCACACGCC TGTACAGCAT 
GGCTATCTCT CACCAAAGCC TCCTACACAG CAGTTAGGCT CTCCCTATAG GCCTCACCAT 
TCACAGTCAC CTCAAGTTGG AACACCTCAG CGAGAGCCTC AAAGAAATTT TTACCCAGCC 
GCACAGAACC TTCAAGCCAG TACTCAGCAG GCAACTTCTG GAGCATTATT TACACAGACA 
CCCTCAGGAC AATCTTCAGC AACATACAGT CAGTTTAATC AGACAAGTCT GAGCAGCACG 
GCACCACCCC CTCCACCCCC TCCACCCCCT TCTTCTTCGT CTTATTATCA AAACCAGCAG 
CCCTCTGCAA ACTTTCAGAA TTATAATCAG CTTAAAGGTA GTCTTTCCCA ACAAACTGTG 
TTTACATCAG GACCAAATCA AGCACTTCCT GGCACCACAA GCCAGCAAAC AGTTCCAGGA 
CACCACGTTA CTCCAGGGCA TTTTTTGCCT TCTCAGAACC CTACTGTTCA CCATCAGACT 
GCTGCTGCTG TGGTCCCACC CCCTCCTCCA CCCCCACCTG CTCCAGGACC ACACCTCGTA 
CAACAGCCAA GCTCCCATCA GCCACACTCA GTAGCACATG TGGTGGGGCC TGTTCACGCT 
GTCACCCCTG GATCGCATAT TCATTCTCAA ACTGCTGGGC ACCATTTGCC ACCACCGCCT 
CCCCCTCCTG GTCCTGCCCC TCATCACCAC CCCCCGCCCC ACCCTTCCAC AGGACTCCAA 
GGTCTACAAG CACAACACCA GCATGTGGTA AACTCAGCCC CTCCACCTCC CCCTCCTCCT 
CCGCCTTCCA GTGTTCTGGC TTCTGGGCAT CACACCACGT CAGCTCAAGC CTTACACCAT 
CCACCTCATC AAGGACCGCC ACTTTTTCCT TCAAGTGCTC ATCCAGCTGT ACCACCGTAT 
CCCTCACAAG CTACACATCA CACCACTCTG GGACTGGGAC CCCAACACCA GCCTTCTGGA 
ACAGGGCCAC ATTGTCCATT ACCAGTTGCA GGTCCTCATC TCCAGCCCCA AGGACCAAAC 
AGTATTCCAA CACCTACCGC TTCAGGGTTC TGTCCTCACC CTGGCTCTGT GGCCCTGCCA 
CATGGGGTCC AAGGACCTCA GCAGGCATCT CCAGTGCCTG GACAGATTCC AATTCACAGA 
GCACAGGTGC CACCGACATT TCAGAACAAT TACCATGGTT CGGGGTGGCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KMT2E ( XM_005205367.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205424.1 Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X6, mRNA.

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CloneID	OBa18521
Clone ID Related Accession (Same CDS sequence)	XM_005205367.3
Accession Version	XM_005205367.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase 2E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205367.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGATTG ACAGGCAGCA TATTCCTGAT ACATATCTTT GTGAACGTTG TCAGCCTAGG 
AGTTTGGATA AAGAGAGGGC GGTGCTACTA CAACGCCGGA AAAGGGAAAA TATGTCAGAT 
GGTGATACCA GTGCAACTGA GAGTGGTGAT GAGGTTCCTG TGGAATTATA TACTGCATTT 
CAGCATACCC CAACATCGAT TACTTTAACT GCTTCAAGGG TTTCTAAGGT TAATGATAAA 
AGAAGGAAAA AAAGTGGGGA GAAAGAACAA AATATTTCAA AATGTAAAAA AGCCTTTCGT 
GAAGGATCTA GGAAATCATC AAGAGTTAAG GGCTCAGCTC CGGAGATTGA TCCTTCATCT 
GATGTTTCAA ATTTTGGATG GGAGACAAAA ATCAAAGCAT GGATGGATCA ATATGAAGAG 
GCAAATAATA ACCAATATAG TGAGAGTGTT CAGAGGGAGG CACAAAGAAT AGCTCTGAGA 
TTGGGCAATG GAAATGACAA AAAAGAGATT AAATCAGATT TGAATACCAA CAATTTGATC 
TTCAAACCTC CTGTAGAGAG CCATATACAA AAAAATAAGA AGATTCTGAA ATCTGCAAAA 
GATTTGCCTC CTGATGCACT TATCATTGAA TACAGAGGGA AGTTTATGTT GAGAGAGCAG 
TTTGAAGCAA ATGGATATTT CTTTAAGAGA CCATACCCTT TTGTTTTATT CTATTCTAAA 
TTTCATGGCC TAGAAATGTG TGTTGATGCA AGGACTTTTG GGAATGAAGC TCGGTTCATC 
AGGCGTTCTT GTACACCCAA TGCAGAGGTG AGGCATGAAA TTGAAGATGG AACCATACAT 
CTTTATATTT ATTCTATACA AAGTATTCCA AAGGGAACCG AAATTACTAT TGCCTTTGAT 
TTTGACTATG GGAATTGTAA GTACAAGGTG GATTGTGCAT GCCTCAAAGA AAATTCAGAG 
TGCCCTGTTC TGAAACGTAG TTCTGAATCC ACGGAGAACA TCAATAGCGG TTATGAGACC 
AGAAGGAAGA AAGGCAAAAA AGAAAAGGAT ATTTCAAAAG AAAAAGACAC ACAAAATCAG 
AATATTACTC TGGATTGTGA AGGAACAACG AACAAAATGA AGAGCCCAGA AACTAAACAG 
AGAAAGCTTT CTCCACTGAG ACTATCAGTA TCAAATAATC AGGAACCAGA TTTTATTGAT 
GATATAGAAG AAAAAACTCC CATCAGCAAT GAGGTAGAAA TGGAGTCTGA GGAACAGATT 
GCAGAGAGGA AAAGGAAGAT GACAAGAGAA GAAAGGAAAA TGGAAGCAAT TCTGCAGGCT 
TTTGCCAGAC TTGAAAAAAG AGAGAAGAGA AGAGAACAAG CTTTGGAAAG AATCAGCACA 
GCCAAAACTG AAGTTAAAAC TGAATGTAAA GATGCACAGA CTGTCAGTGA TGCTGAAGTT 
ATTCAGGAAC AAGCAAAAGA AGAAACTGCT AGCAAGCCAA CCCCAGCCAA AGTAAATAGA 
ACTAAACAGA GAAAAAGTTT TTCTCGGAGC AGGACTCACA TTGGACAGCA GCGTCGGAGA 
CACAGGACTG TCAGTATGTG TTCGGATATC CAACCGTCTT CTCCTGACAT CGAAGTTACC 
TCACAACAAA ACGATGTGGA AAATACTGTA CTTGCAATAG AACCAGAAAC TGAAACTGCA 
CTAACAGAAA TAATTACTGA AAATGAAGTT CCAGTACTTA ATAAATGTCC AACAAAGTAC 
CCTAAAACAA AGAAGCACTT GGTCAGTGAA TGGTTAAGTG AGAAGAATGA GAAGACAGGA 
AAACCTTCAG ACAGCCTTTC AGAGAGGCCG CTGCGCATAA CTACAGATCC TGAAGTGCTA 
GCTACACAGC TCAATTCTTT GCCAGGTCTC ACTTACAGTC CACATGTATA CTCTACTCCT 
AAGCACTATA TTAGATTCAC TTCACCATTC CTTTCGGAAA AAAGGAGAAG AAAAGAACCT 
CCTGAAAACA TATCTGGCTC ATGCAAGAAG CGGTGGTTGA AACAAGCTCT GGAAGAGGAA 
AACTCAGCAA TTTTACATAG ATTTAATTCG CCCTGTCAAG AAAGATCCAG AAGTCCTACA 
GTCAATGGTG AAAATAAAAG TCCACTACTA TTAAATGATA GCTGTTCCCT TCCAGATTTA 
ACTACACCAC TGAAAAAACG AAGACTTTAT CAGTTGCTAG ATTCTGCTTA CTCAGAAACC 
TCCACACCTA CTCCTTCACC GTATGCTACA CCAACTCACA CAGATATTAC TCCTACAGAC 
CCATCATTTT CCACTCCTCC ACGGATAAAG TCAGATGATG AAGCTTGTAG AAATGGTTAT 
AAGCCCATAT ATTCACCAGT TACCCCAGTA ACTCCTGGCA CACCAGGAAA TACCATGCAC 
TTTGAGAATA TTTCTTCCCC AGAAAGTTCT CCAGAAATTA AGAGACGCAC TTATAGTCAA 
GAGGGATATG ACAGATCGTC AACCATGTTA ACGTTGGGGC CTTTTAGAAA TTCTAACTTA 
ACTGAACTGG GTCTGCAAGA AATAAAGACT ATTGGCTATG GCAGCCCTAG GAGTAGGACT 
GAAATCAGCA GGCAGTGCCC TGGAGAAAAG GAACCGGTGT CAGATCTTCA ACTGGGACTC 
GATGCAGTCG AGCAGACCGC CTTACATAAA ACCATGGAAG CAGCTGCACA CGACAGGACT 
GAGGCTGCCA GCCAGCTGGA GGCTGCTCAC TCGGGGCGGG GCCCTCTGTA TTCCTCCTGG 
GTAAAGAGTC CTGAGAGAAC AGGCGTTAAC TTCTCAGTAA ATTCCAACTT GAGGGACCTG 
ACACCCTCAC ATCAGTTGGA GGTTGGAGGC GGCTTCCGAA TAAGTGAGTC AAAGTGCCTG 
ATGCAGGATG ATACTAGAGG CATGTTTATG GAAACACCCG TGTTTTGTAC TTCAGAAGAT 
GGGCTTGTAT CTGGTTTCGG ACGGACTGTT AATGACAATT TGATCGACGG AAGTTGCACA 
CCCCAGAATC CACCACAAAA GAAAAAGGTT TCTCTATTAG AATACCGGAA GAGACAACGT 
GAAGCTAGGA AGAGCGGCTC TAAGACGGAG CACTTTGCGC TGGTTAGTGT ATCACCTCAC 
ACAAGTGGCA ACTTGAGCAG CAGCACTGGT GATGGGTGTG CCCACAGGAG TGAAAATGGG 
GAGCAGGTAG AAAGCGCTGC TAGCCTACCT TTACCAACAC CAGCTACAGT TTATCATGCT 
ACTTCGGAAG AAACCAGCAA TAACAGTACT GTTAAGGAAG TGTCTGCCAG TGAAAAGAAT 
GAACCAGAAG TTCAATGGAC GGCCTCAACT TCAGTGGAGC AAGTGAGAGA GAGGAGTTAT 
CAGAGAGCTC TGCTTCTCAG TGATCACCGA AAAGATAAAG ACAGTGGGGC AGAGTCACCA 
TGTGTCCCCT GTTCACCGAA TCATGTTCCG TCTTCTCCTT CATCTCATTC AAATCACATT 
CCCCAGTTGC AACCCAAGGG CCCAGTCCCT TCTTTCAGTG AACTTACGGA AGATCCTGAT 
CCTGAAAATC TAGAACCCAC AACTACAAAT GAATGCCCAT CCCCAGATAC TTCCCAAAGT 
ACTTGTAAAA GTCCTTCAAA AATGAGCAAG CCTGGTTCAC CTGGACCTGT AATTCCTGTT 
CAGCCACATG GGAAAATACT TACAAAACCA GATTCCCATT GGGAGGGAAC AGTTATTGTA 
TCTGAAGCTG AGAATGGTGT CCACCTGAAA ACAGAACTCC ATCAAAAACA GCTGTCAAGT 
AACCCCCAAG CACTTTCAAA GAACCATCCT CCACAGCCAC TCGTTCGCTG TTCATCTGAG 
CAGCTTTCAC AAAAGCTGCC TTCTGCACCC ATGAAGTTGC ACTGTCCTCC GTCACCTCAC 
GTAGAAAATC CTCCAAAGTC ATCGACGCCT CACACGCCTG TACAGCATGG CTATCTCTCA 
CCAAAGCCTC CTACACAGCA GTTAGGCTCT CCCTATAGGC CTCACCATTC ACAGTCACCT 
CAAGTTGGAA CACCTCAGCG AGAGCCTCAA AGAAATTTTT ACCCAGCCGC ACAGAACCTT 
CAAGCCAGTA CTCAGCAGGC AACTTCTGGA GCATTATTTA CACAGACACC CTCAGGACAA 
TCTTCAGCAA CATACAGTCA GTTTAATCAG ACAAGTCTGA GCAGCACGGC ACCACCCCCT 
CCACCCCCTC CACCCCCTTC TTCTTCGTCT TATTATCAAA ACCAGCAGCC CTCTGCAAAC 
TTTCAGAATT ATAATCAGCT TAAAGGTAGT CTTTCCCAAC AAACTGTGTT TACATCAGGA 
CCAAATCAAG CACTTCCTGG CACCACAAGC CAGCAAACAG TTCCAGGACA CCACGTTACT 
CCAGGGCATT TTTTGCCTTC TCAGAACCCT ACTGTTCACC ATCAGACTGC TGCTGCTGTG 
GTCCCACCCC CTCCTCCACC CCCACCTGCT CCAGGACCAC ACCTCGTACA ACAGCCAAGC 
TCCCATCAGC CACACTCAGT AGCACATGTG GTGGGGCCTG TTCACGCTGT CACCCCTGGA 
TCGCATATTC ATTCTCAAAC TGCTGGGCAC CATTTGCCAC CACCGCCTCC CCCTCCTGGT 
CCTGCCCCTC ATCACCACCC CCCGCCCCAC CCTTCCACAG GACTCCAAGG TCTACAAGCA 
CAACACCAGC ATGTGGTAAA CTCAGCCCCT CCACCTCCCC CTCCTCCTCC GCCTTCCAGT 
GTTCTGGCTT CTGGGCATCA CACCACGTCA GCTCAAGCCT TACACCATCC ACCTCATCAA 
GGACCGCCAC TTTTTCCTTC AAGTGCTCAT CCAGCTGTAC CACCGTATCC CTCACAAGCT 
ACACATCACA CCACTCTGGG ACTGGGACCC CAACACCAGC CTTCTGGAAC AGGGCCACAT 
TGTCCATTAC CAGTTGCAGG TCCTCATCTC CAGCCCCAAG GACCAAACAG TATTCCAACA 
CCTACCGCTT CAGGGTTCTG TCCTCACCCT GGCTCTGTGG CCCTGCCACA TGGGGTCCAA 
GGACCTCAGC AGGCATCTCC AGTGCCTGGA CAGATTCCAA TTCACAGAGC ACAGGTGCCA 
CCGACATTTC AGAACAATTA CCATGGTTCG GGGTGGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205424.1
CDS	205..5346
Translation	MGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQNISKCKKAFREGSRKSSRVKGSAPEIDPSSDVSNFGWETKIKAWMDQYEEANNNQYSESVQREAQRIALRLGNGNDKKEIKSDLNTNNLIFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEDGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENSECPVLKRSSESTENINSGYETRRKKGKKEKDISKEKDTQNQNITLDCEGTTNKMKSPETKQRKLSPLRLSVSNNQEPDFIDDIEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKTECKDAQTVSDAEVIQEQAKEETASKPTPAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDIEVTSQQNDVENTVLAIEPETETALTEIITENEVPVLNKCPTKYPKTKKHLVSEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKRRRKEPPENISGSCKKRWLKQALEEENSAILHRFNSPCQERSRSPTVNGENKSPLLLNDSCSLPDLTTPLKKRRLYQLLDSAYSETSTPTPSPYATPTHTDITPTDPSFSTPPRIKSDDEACRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRRTYSQEGYDRSSTMLTLGPFRNSNLTELGLQEIKTIGYGSPRSRTEISRQCPGEKEPVSDLQLGLDAVEQTALHKTMEAAAHDRTEAASQLEAAHSGRGPLYSSWVKSPERTGVNFSVNSNLRDLTPSHQLEVGGGFRISESKCLMQDDTRGMFMETPVFCTSEDGLVSGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKTEHFALVSVSPHTSGNLSSSTGDGCAHRSENGEQVESAASLPLPTPATVYHATSEETSNNSTVKEVSASEKNEPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSGAESPCVPCSPNHVPSSPSSHSNHIPQLQPKGPVPSFSELTEDPDPENLEPTTTNECPSPDTSQSTCKSPSKMSKPGSPGPVIPVQPHGKILTKPDSHWEGTVIVSEAENGVHLKTELHQKQLSSNPQALSKNHPPQPLVRCSSEQLSQKLPSAPMKLHCPPSPHVENPPKSSTPHTPVQHGYLSPKPPTQQLGSPYRPHHSQSPQVGTPQREPQRNFYPAAQNLQASTQQATSGALFTQTPSGQSSATYSQFNQTSLSSTAPPPPPPPPPSSSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQQTVPGHHVTPGHFLPSQNPTVHHQTAAAVVPPPPPPPPAPGPHLVQQPSSHQPHSVAHVVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPSTGLQGLQAQHQHVVNSAPPPPPPPPPSSVLASGHHTTSAQALHHPPHQGPPLFPSSAHPAVPPYPSQATHHTTLGLGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPGSVALPHGVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_005205367.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205367.3

ORF Insert Sequence:

ATGGGGATTG ACAGGCAGCA TATTCCTGAT ACATATCTTT GTGAACGTTG TCAGCCTAGG 
AGTTTGGATA AAGAGAGGGC GGTGCTACTA CAACGCCGGA AAAGGGAAAA TATGTCAGAT 
GGTGATACCA GTGCAACTGA GAGTGGTGAT GAGGTTCCTG TGGAATTATA TACTGCATTT 
CAGCATACCC CAACATCGAT TACTTTAACT GCTTCAAGGG TTTCTAAGGT TAATGATAAA 
AGAAGGAAAA AAAGTGGGGA GAAAGAACAA AATATTTCAA AATGTAAAAA AGCCTTTCGT 
GAAGGATCTA GGAAATCATC AAGAGTTAAG GGCTCAGCTC CGGAGATTGA TCCTTCATCT 
GATGTTTCAA ATTTTGGATG GGAGACAAAA ATCAAAGCAT GGATGGATCA ATATGAAGAG 
GCAAATAATA ACCAATATAG TGAGAGTGTT CAGAGGGAGG CACAAAGAAT AGCTCTGAGA 
TTGGGCAATG GAAATGACAA AAAAGAGATT AAATCAGATT TGAATACCAA CAATTTGATC 
TTCAAACCTC CTGTAGAGAG CCATATACAA AAAAATAAGA AGATTCTGAA ATCTGCAAAA 
GATTTGCCTC CTGATGCACT TATCATTGAA TACAGAGGGA AGTTTATGTT GAGAGAGCAG 
TTTGAAGCAA ATGGATATTT CTTTAAGAGA CCATACCCTT TTGTTTTATT CTATTCTAAA 
TTTCATGGCC TAGAAATGTG TGTTGATGCA AGGACTTTTG GGAATGAAGC TCGGTTCATC 
AGGCGTTCTT GTACACCCAA TGCAGAGGTG AGGCATGAAA TTGAAGATGG AACCATACAT 
CTTTATATTT ATTCTATACA AAGTATTCCA AAGGGAACCG AAATTACTAT TGCCTTTGAT 
TTTGACTATG GGAATTGTAA GTACAAGGTG GATTGTGCAT GCCTCAAAGA AAATTCAGAG 
TGCCCTGTTC TGAAACGTAG TTCTGAATCC ACGGAGAACA TCAATAGCGG TTATGAGACC 
AGAAGGAAGA AAGGCAAAAA AGAAAAGGAT ATTTCAAAAG AAAAAGACAC ACAAAATCAG 
AATATTACTC TGGATTGTGA AGGAACAACG AACAAAATGA AGAGCCCAGA AACTAAACAG 
AGAAAGCTTT CTCCACTGAG ACTATCAGTA TCAAATAATC AGGAACCAGA TTTTATTGAT 
GATATAGAAG AAAAAACTCC CATCAGCAAT GAGGTAGAAA TGGAGTCTGA GGAACAGATT 
GCAGAGAGGA AAAGGAAGAT GACAAGAGAA GAAAGGAAAA TGGAAGCAAT TCTGCAGGCT 
TTTGCCAGAC TTGAAAAAAG AGAGAAGAGA AGAGAACAAG CTTTGGAAAG AATCAGCACA 
GCCAAAACTG AAGTTAAAAC TGAATGTAAA GATGCACAGA CTGTCAGTGA TGCTGAAGTT 
ATTCAGGAAC AAGCAAAAGA AGAAACTGCT AGCAAGCCAA CCCCAGCCAA AGTAAATAGA 
ACTAAACAGA GAAAAAGTTT TTCTCGGAGC AGGACTCACA TTGGACAGCA GCGTCGGAGA 
CACAGGACTG TCAGTATGTG TTCGGATATC CAACCGTCTT CTCCTGACAT CGAAGTTACC 
TCACAACAAA ACGATGTGGA AAATACTGTA CTTGCAATAG AACCAGAAAC TGAAACTGCA 
CTAACAGAAA TAATTACTGA AAATGAAGTT CCAGTACTTA ATAAATGTCC AACAAAGTAC 
CCTAAAACAA AGAAGCACTT GGTCAGTGAA TGGTTAAGTG AGAAGAATGA GAAGACAGGA 
AAACCTTCAG ACAGCCTTTC AGAGAGGCCG CTGCGCATAA CTACAGATCC TGAAGTGCTA 
GCTACACAGC TCAATTCTTT GCCAGGTCTC ACTTACAGTC CACATGTATA CTCTACTCCT 
AAGCACTATA TTAGATTCAC TTCACCATTC CTTTCGGAAA AAAGGAGAAG AAAAGAACCT 
CCTGAAAACA TATCTGGCTC ATGCAAGAAG CGGTGGTTGA AACAAGCTCT GGAAGAGGAA 
AACTCAGCAA TTTTACATAG ATTTAATTCG CCCTGTCAAG AAAGATCCAG AAGTCCTACA 
GTCAATGGTG AAAATAAAAG TCCACTACTA TTAAATGATA GCTGTTCCCT TCCAGATTTA 
ACTACACCAC TGAAAAAACG AAGACTTTAT CAGTTGCTAG ATTCTGCTTA CTCAGAAACC 
TCCACACCTA CTCCTTCACC GTATGCTACA CCAACTCACA CAGATATTAC TCCTACAGAC 
CCATCATTTT CCACTCCTCC ACGGATAAAG TCAGATGATG AAGCTTGTAG AAATGGTTAT 
AAGCCCATAT ATTCACCAGT TACCCCAGTA ACTCCTGGCA CACCAGGAAA TACCATGCAC 
TTTGAGAATA TTTCTTCCCC AGAAAGTTCT CCAGAAATTA AGAGACGCAC TTATAGTCAA 
GAGGGATATG ACAGATCGTC AACCATGTTA ACGTTGGGGC CTTTTAGAAA TTCTAACTTA 
ACTGAACTGG GTCTGCAAGA AATAAAGACT ATTGGCTATG GCAGCCCTAG GAGTAGGACT 
GAAATCAGCA GGCAGTGCCC TGGAGAAAAG GAACCGGTGT CAGATCTTCA ACTGGGACTC 
GATGCAGTCG AGCAGACCGC CTTACATAAA ACCATGGAAG CAGCTGCACA CGACAGGACT 
GAGGCTGCCA GCCAGCTGGA GGCTGCTCAC TCGGGGCGGG GCCCTCTGTA TTCCTCCTGG 
GTAAAGAGTC CTGAGAGAAC AGGCGTTAAC TTCTCAGTAA ATTCCAACTT GAGGGACCTG 
ACACCCTCAC ATCAGTTGGA GGTTGGAGGC GGCTTCCGAA TAAGTGAGTC AAAGTGCCTG 
ATGCAGGATG ATACTAGAGG CATGTTTATG GAAACACCCG TGTTTTGTAC TTCAGAAGAT 
GGGCTTGTAT CTGGTTTCGG ACGGACTGTT AATGACAATT TGATCGACGG AAGTTGCACA 
CCCCAGAATC CACCACAAAA GAAAAAGGTT TCTCTATTAG AATACCGGAA GAGACAACGT 
GAAGCTAGGA AGAGCGGCTC TAAGACGGAG CACTTTGCGC TGGTTAGTGT ATCACCTCAC 
ACAAGTGGCA ACTTGAGCAG CAGCACTGGT GATGGGTGTG CCCACAGGAG TGAAAATGGG 
GAGCAGGTAG AAAGCGCTGC TAGCCTACCT TTACCAACAC CAGCTACAGT TTATCATGCT 
ACTTCGGAAG AAACCAGCAA TAACAGTACT GTTAAGGAAG TGTCTGCCAG TGAAAAGAAT 
GAACCAGAAG TTCAATGGAC GGCCTCAACT TCAGTGGAGC AAGTGAGAGA GAGGAGTTAT 
CAGAGAGCTC TGCTTCTCAG TGATCACCGA AAAGATAAAG ACAGTGGGGC AGAGTCACCA 
TGTGTCCCCT GTTCACCGAA TCATGTTCCG TCTTCTCCTT CATCTCATTC AAATCACATT 
CCCCAGTTGC AACCCAAGGG CCCAGTCCCT TCTTTCAGTG AACTTACGGA AGATCCTGAT 
CCTGAAAATC TAGAACCCAC AACTACAAAT GAATGCCCAT CCCCAGATAC TTCCCAAAGT 
ACTTGTAAAA GTCCTTCAAA AATGAGCAAG CCTGGTTCAC CTGGACCTGT AATTCCTGTT 
CAGCCACATG GGAAAATACT TACAAAACCA GATTCCCATT GGGAGGGAAC AGTTATTGTA 
TCTGAAGCTG AGAATGGTGT CCACCTGAAA ACAGAACTCC ATCAAAAACA GCTGTCAAGT 
AACCCCCAAG CACTTTCAAA GAACCATCCT CCACAGCCAC TCGTTCGCTG TTCATCTGAG 
CAGCTTTCAC AAAAGCTGCC TTCTGCACCC ATGAAGTTGC ACTGTCCTCC GTCACCTCAC 
GTAGAAAATC CTCCAAAGTC ATCGACGCCT CACACGCCTG TACAGCATGG CTATCTCTCA 
CCAAAGCCTC CTACACAGCA GTTAGGCTCT CCCTATAGGC CTCACCATTC ACAGTCACCT 
CAAGTTGGAA CACCTCAGCG AGAGCCTCAA AGAAATTTTT ACCCAGCCGC ACAGAACCTT 
CAAGCCAGTA CTCAGCAGGC AACTTCTGGA GCATTATTTA CACAGACACC CTCAGGACAA 
TCTTCAGCAA CATACAGTCA GTTTAATCAG ACAAGTCTGA GCAGCACGGC ACCACCCCCT 
CCACCCCCTC CACCCCCTTC TTCTTCGTCT TATTATCAAA ACCAGCAGCC CTCTGCAAAC 
TTTCAGAATT ATAATCAGCT TAAAGGTAGT CTTTCCCAAC AAACTGTGTT TACATCAGGA 
CCAAATCAAG CACTTCCTGG CACCACAAGC CAGCAAACAG TTCCAGGACA CCACGTTACT 
CCAGGGCATT TTTTGCCTTC TCAGAACCCT ACTGTTCACC ATCAGACTGC TGCTGCTGTG 
GTCCCACCCC CTCCTCCACC CCCACCTGCT CCAGGACCAC ACCTCGTACA ACAGCCAAGC 
TCCCATCAGC CACACTCAGT AGCACATGTG GTGGGGCCTG TTCACGCTGT CACCCCTGGA 
TCGCATATTC ATTCTCAAAC TGCTGGGCAC CATTTGCCAC CACCGCCTCC CCCTCCTGGT 
CCTGCCCCTC ATCACCACCC CCCGCCCCAC CCTTCCACAG GACTCCAAGG TCTACAAGCA 
CAACACCAGC ATGTGGTAAA CTCAGCCCCT CCACCTCCCC CTCCTCCTCC GCCTTCCAGT 
GTTCTGGCTT CTGGGCATCA CACCACGTCA GCTCAAGCCT TACACCATCC ACCTCATCAA 
GGACCGCCAC TTTTTCCTTC AAGTGCTCAT CCAGCTGTAC CACCGTATCC CTCACAAGCT 
ACACATCACA CCACTCTGGG ACTGGGACCC CAACACCAGC CTTCTGGAAC AGGGCCACAT 
TGTCCATTAC CAGTTGCAGG TCCTCATCTC CAGCCCCAAG GACCAAACAG TATTCCAACA 
CCTACCGCTT CAGGGTTCTG TCCTCACCCT GGCTCTGTGG CCCTGCCACA TGGGGTCCAA 
GGACCTCAGC AGGCATCTCC AGTGCCTGGA CAGATTCCAA TTCACAGAGC ACAGGTGCCA 
CCGACATTTC AGAACAATTA CCATGGTTCG GGGTGGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KMT2E
Entrez Gene ID	539121
Full Name	lysine methyltransferase 2E
Synonyms	MLL5
General protein information	Preferred Names lysine methyltransferase 2E Names histone-lysine N-methyltransferase 2E myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Bos taurus (cattle)	MLL5	NP_001075920.2
Mus musculus (house mouse)	Kmt2e	NP_081260.1
Rattus norvegicus (Norway rat)	Kmt2e	NP_001094321.1
Pan troglodytes (chimpanzee)	MLL5	XP_003318779.1
Gallus gallus (chicken)	MLL5	XP_003640389.1
Danio rerio (zebrafish)	kmt2e	XP_692965.5
Xenopus tropicalis (tropical clawed frog)	kmt2e	XP_004913074.1
Homo sapiens (human)	KMT2E	NP_891847.1
	LOC697233	XP_002808408.1
Canis lupus familiaris (dog)	KMT2E	XP_533102.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

KMT2E cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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