KMT2E cDNA ORF clone, Bos taurus(cattle)

The following KMT2E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KMT2E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa233777 XM_005205366.4
Latest version!
Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa233778 XM_005205367.4
Latest version!
Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa233779 XM_024990819.1
Latest version!
Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa01901 NM_001082451.3
Latest version!
Bos taurus lysine methyltransferase 2E (KMT2E), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa01901 XM_005205364.4
Latest version!
Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa01901 XM_005205365.4
Latest version!
Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa01901 XM_005205363.4
Latest version!
Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa01901 XM_005205362.4
Latest version!
Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa18519 XM_005205364.3
Latest version!
Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa18519 XM_005205363.3
Latest version!
Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa18519 XM_005205362.3
Latest version!
Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa18519 XM_005205365.3
Latest version!
Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa18520 XM_005205366.3
Latest version!
Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa18521 XM_005205367.3
Latest version!
Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
OBa01901 NM_001082451.2 Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa233777
    Clone ID Related Accession (Same CDS sequence) XM_005205366.4
    Accession Version XM_005205366.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5454bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive histone-lysine N-methyltransferase 2E isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205366.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGATCCTG ATCCTGAAAA TCTAGAACCC ACAACTACAA ATGAATGCCC ATCCCCAGAT
    ACTTCCCAAA GTACTTGTAA AAGTCCTTCA AAAATGAGCA AGCCTGGTTC ACCTGGACCT
    GTAATTCCTG TTCAGCCACA TGGGAAAATA CTTACAAAAC CAGATTCCCA TTGGGAGGGA
    ACAGTTATTG TATCTGAAGC TGAGAATGGT GTCCACCTGA AAACAGAACT CCATCAAAAA
    CAGCTGTCAA GTAACCCCCA AGCACTTTCA AAGAACCATC CTCCACAGCC ACTCGTTCGC
    TGTTCATCTG AGCAGCTTTC ACAAAAGCTG CCTTCTGCAC CCATGAAGTT GCACTGTCCT
    CCGTCACCTC ACGTAGAAAA TCCTCCAAAG TCATCGACGC CTCACACGCC TGTACAGCAT
    GGCTATCTCT CACCAAAGCC GCCTACACAG CAGTTAGGCT CTCCCTATAG GCCTCACCAT
    TCACAGTCAC CTCAAGTTGG AACACCTCAG CGAGAGCCTC AAAGAAATTT TTACCCAGCC
    GCACAGAACC TTCAAGCCAG TACTCAGCAG GCAACTTCTG GAGCATTATT TACACAGACA
    CCCTCAGGAC AATCTTCAGC AACATACAGT CAGTTTAATC AGACAAGTCT GAGCAGCACG
    GCACCACCCC CTCCACCCCC TCCACCCCCT TCTTCTTCGT CTTATTATCA AAACCAGCAG
    CCCTCTGCAA ACTTTCAGAA TTATAATCAG CTTAAAGGTA GTCTTTCCCA ACAAACTGTG
    TTTACATCAG GACCAAATCA AGCACTTCCT GGCACCACAA GCCAGCAAAC AGTTCCAGGA
    CACCACGTTA CTCCAGGGCA TTTTTTGCCT TCTCAGAACC CTACTGTTCA CCATCAGACT
    GCTGCTGCTG TGGTCCCACC CCCTCCTCCA CCCCCACCTG CTCCAGGACC ACACCTCGTA
    CAACAGCCAA GCTCCCATCA GCCACACTCA GTAGCACATG TGGTGGGGCC TGTTCACGCT
    GTCACCCCTG GATCGCATAT TCATTCTCAA ACTGCTGGGC ACCATTTGCC ACCACCGCCT
    CCCCCTCCTG GTCCTGCCCC TCATCACCAC CCCCCGCCCC ACCCTTCCAC AGGACTCCAA
    GGTCTACAAG CACAACACCA GCATGTGGTA AACTCAGCCC CTCCACCTCC CCCTCCTCCT
    CCGCCTTCCA GTGTTCTGGC TTCTGGGCAT CACACCACGT CAGCTCAAGC CTTACACCAT
    CCACCTCATC AAGGACCGCC ACTTTTTCCT TCAAGTGCTC ATCCAGCTGT ACCACCGTAT
    CCCTCACAAG CTACACATCA CACCACTCTG GGACTGGGAC CCCAACACCA GCCTTCTGGA
    ACAGGGCCAC ATTGTCCATT ACCAGTTGCA GGTCCTCATC TCCAGCCCCA AGGACCAAAC
    AGTATTCCAA CACCTACCGC TTCAGGGTTC TGTCCTCACC CTGGCTCTGT GGCCCTGCCA
    CATGGGGTCC AAGGACCTCA GCAGGCATCT CCAGTGCCTG GACAGATTCC AATTCACAGA
    GCACAGGTGC CACCGACATT TCAGAACAAT TACCATGGTT CGGGGTGGCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205423.1
    CDS510..5963
    Translation

    Target ORF information:

    RefSeq Version XM_005205366.4
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205366.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGATCCTG ATCCTGAAAA TCTAGAACCC ACAACTACAA ATGAATGCCC ATCCCCAGAT
    ACTTCCCAAA GTACTTGTAA AAGTCCTTCA AAAATGAGCA AGCCTGGTTC ACCTGGACCT
    GTAATTCCTG TTCAGCCACA TGGGAAAATA CTTACAAAAC CAGATTCCCA TTGGGAGGGA
    ACAGTTATTG TATCTGAAGC TGAGAATGGT GTCCACCTGA AAACAGAACT CCATCAAAAA
    CAGCTGTCAA GTAACCCCCA AGCACTTTCA AAGAACCATC CTCCACAGCC ACTCGTTCGC
    TGTTCATCTG AGCAGCTTTC ACAAAAGCTG CCTTCTGCAC CCATGAAGTT GCACTGTCCT
    CCGTCACCTC ACGTAGAAAA TCCTCCAAAG TCATCGACGC CTCACACGCC TGTACAGCAT
    GGCTATCTCT CACCAAAGCC GCCTACACAG CAGTTAGGCT CTCCCTATAG GCCTCACCAT
    TCACAGTCAC CTCAAGTTGG AACACCTCAG CGAGAGCCTC AAAGAAATTT TTACCCAGCC
    GCACAGAACC TTCAAGCCAG TACTCAGCAG GCAACTTCTG GAGCATTATT TACACAGACA
    CCCTCAGGAC AATCTTCAGC AACATACAGT CAGTTTAATC AGACAAGTCT GAGCAGCACG
    GCACCACCCC CTCCACCCCC TCCACCCCCT TCTTCTTCGT CTTATTATCA AAACCAGCAG
    CCCTCTGCAA ACTTTCAGAA TTATAATCAG CTTAAAGGTA GTCTTTCCCA ACAAACTGTG
    TTTACATCAG GACCAAATCA AGCACTTCCT GGCACCACAA GCCAGCAAAC AGTTCCAGGA
    CACCACGTTA CTCCAGGGCA TTTTTTGCCT TCTCAGAACC CTACTGTTCA CCATCAGACT
    GCTGCTGCTG TGGTCCCACC CCCTCCTCCA CCCCCACCTG CTCCAGGACC ACACCTCGTA
    CAACAGCCAA GCTCCCATCA GCCACACTCA GTAGCACATG TGGTGGGGCC TGTTCACGCT
    GTCACCCCTG GATCGCATAT TCATTCTCAA ACTGCTGGGC ACCATTTGCC ACCACCGCCT
    CCCCCTCCTG GTCCTGCCCC TCATCACCAC CCCCCGCCCC ACCCTTCCAC AGGACTCCAA
    GGTCTACAAG CACAACACCA GCATGTGGTA AACTCAGCCC CTCCACCTCC CCCTCCTCCT
    CCGCCTTCCA GTGTTCTGGC TTCTGGGCAT CACACCACGT CAGCTCAAGC CTTACACCAT
    CCACCTCATC AAGGACCGCC ACTTTTTCCT TCAAGTGCTC ATCCAGCTGT ACCACCGTAT
    CCCTCACAAG CTACACATCA CACCACTCTG GGACTGGGAC CCCAACACCA GCCTTCTGGA
    ACAGGGCCAC ATTGTCCATT ACCAGTTGCA GGTCCTCATC TCCAGCCCCA AGGACCAAAC
    AGTATTCCAA CACCTACCGC TTCAGGGTTC TGTCCTCACC CTGGCTCTGT GGCCCTGCCA
    CATGGGGTCC AAGGACCTCA GCAGGCATCT CCAGTGCCTG GACAGATTCC AATTCACAGA
    GCACAGGTGC CACCGACATT TCAGAACAAT TACCATGGTT CGGGGTGGCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233778
    Clone ID Related Accession (Same CDS sequence) XM_005205367.4
    Accession Version XM_005205367.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive histone-lysine N-methyltransferase 2E isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205367.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGATTG ACAGGCAGCA TATTCCTGAT ACATATCTTT GTGAACGTTG TCAGCCTAGG 
    AGTTTGGATA AAGAGAGGGC GGTGCTACTA CAACGCCGGA AAAGGGAAAA TATGTCAGAT
    GGTGATACCA GTGCAACTGA GAGTGGTGAT GAGGTTCCTG TGGAATTATA TACTGCATTT
    CAGCATACCC CAACATCGAT TACTTTAACT GCTTCAAGGG TTTCTAAGGT TAATGATAAA
    AGAAGGAAAA AAAGTGGGGA GAAAGAACAA AATATTTCAA AATGTAAAAA AGCCTTTCGT
    GAAGGATCTA GGAAATCATC AAGAGTTAAG GGCTCAGCTC CGGAGATTGA TCCTTCATCT
    GATGTTTCAA ATTTTGGATG GGAGACAAAA ATCAAAGCAT GGATGGATCA ATATGAAGAG
    GCAAATAATA ACCAATATAG TGAGAGTGTT CAGAGGGAGG CACAAAGAAT AGCTCTGAGA
    TTGGGCAATG GAAATGACAA AAAAGAGATT AAATCAGATT TGAATACCAA CAATTTGATC
    TTCAAACCTC CTGTAGAGAG CCATATACAA AAAAATAAGA AGATTCTGAA ATCTGCAAAA
    GATTTGCCTC CTGATGCACT TATCATTGAA TACAGAGGGA AGTTTATGTT GAGAGAGCAG
    TTTGAAGCAA ATGGATATTT CTTTAAGAGA CCATACCCTT TTGTTTTATT CTATTCTAAA
    TTTCATGGCC TAGAAATGTG TGTTGATGCA AGGACTTTTG GGAATGAAGC TCGGTTCATC
    AGGCGTTCTT GTACACCCAA TGCAGAGGTG AGGCATGAAA TTGAAGATGG AACCATACAT
    CTTTATATTT ATTCTATACA AAGTATTCCA AAGGGAACCG AAATTACTAT TGCCTTTGAT
    TTTGACTATG GGAATTGTAA GTACAAGGTG GATTGTGCAT GCCTCAAAGA AAATTCAGAG
    TGCCCTGTTC TGAAACGTAG TTCTGAATCC ACGGAGAACA TCAATAGCGG TTATGAGACC
    AGAAGGAAGA AAGGCAAAAA AGAAAAGGAT ATTTCAAAAG AAAAAGACAC ACAAAATCAG
    AATATTACTC TGGATTGTGA AGGAACAACG AACAAAATGA AGAGCCCAGA AACTAAACAG
    AGAAAGCTTT CTCCACTGAG ACTATCAGTA TCAAATAATC AGGAACCAGA TTTTATTGAT
    GATATAGAAG AAAAAACTCC CATCAGCAAT GAGGTAGAAA TGGAGTCTGA GGAACAGATT
    GCAGAGAGGA AAAGGAAGAT GACAAGAGAA GAAAGGAAAA TGGAAGCAAT TCTGCAGGCT
    TTTGCCAGAC TTGAAAAAAG AGAGAAGAGA AGAGAACAAG CTTTGGAAAG AATCAGCACA
    GCCAAAACTG AAGTTAAAAC TGAATGTAAA GATGCACAGA CTGTCAGTGA TGCTGAAGTT
    ATTCAGGAAC AAGCAAAAGA AGAAACTGCT AGCAAGCCAA CCCCAGCCAA AGTAAATAGA
    ACTAAACAGA GAAAAAGTTT TTCTCGGAGC AGGACTCACA TTGGACAGCA GCGTCGGAGA
    CACAGGACTG TCAGTATGTG TTCGGATATC CAACCGTCTT CTCCTGACAT CGAAGTTACC
    TCACAACAAA ACGATGTGGA AAATACTGTA CTTGCAATAG AACCAGAAAC TGAAACTGCA
    CTAACAGAAA TAATTACTGA AAATGAAGTT CCAGTACTTA ATAAATGTCC AACAAAGTAC
    CCTAAAACAA AGAAGCACTT GGTCAGTGAA TGGTTAAGTG AGAAGAATGA GAAGACAGGA
    AAACCTTCAG ACAGCCTTTC AGAGAGGCCG CTGCGCATAA CTACAGATCC TGAAGTGCTA
    GCTACACAGC TCAATTCTTT GCCAGGTCTC ACTTACAGTC CACATGTATA CTCTACTCCT
    AAGCACTATA TTAGATTCAC TTCACCATTC CTTTCGGAAA AAAGGAGAAG AAAAGAACCT
    CCTGAAAACA TATCTGGCTC ATGCAAGAAG CGGTGGTTGA AACAAGCTCT GGAAGAGGAA
    AACTCAGCAA TTTTACATAG ATTTAATTCG CCCTGTCAAG AAAGATCCAG AAGTCCTACA
    GTCAATGGTG AAAATAAAAG TCCACTACTA TTAAATGATA GCTGTTCCCT TCCAGATTTA
    ACTACACCAC TGAAAAAACG AAGACTTTAT CAGTTGCTAG ATTCTGCTTA CTCAGAAACC
    TCCACACCTA CTCCTTCACC GTATGCTACA CCAACTCACA CAGATATTAC TCCTACAGAC
    CCATCATTTT CCACTCCTCC ACGGATAAAG TCAGATGATG AAGCTTGTAG AAATGGTTAT
    AAGCCCATAT ATTCACCAGT TACCCCAGTA ACTCCTGGCA CACCAGGAAA TACCATGCAC
    TTTGAGAATA TTTCTTCCCC AGAAAGTTCT CCAGAAATTA AGAGACGCAC TTATAGTCAA
    GAGGGATATG ACAGATCGTC AACCATGTTA ACGTTGGGGC CTTTTAGAAA TTCTAACTTA
    ACTGAACTGG GTCTGCAAGA AATAAAGACT ATTGGCTATG GCAGCCCTAG GAGTAGGACT
    GAAATCAGCA GGCAGTGCCC TGGAGAAAAG GAACCGGTGT CAGATCTTCA ACTGGGACTC
    GATGCAGTCG AGCAGACCGC CTTACATAAA ACCATGGAAG CAGCTGCACA CGACAGGACT
    GAGGCTGCCA GCCAGCTGGA GGCTGCTCAC TCGGGGCGGG GCCCTCTGTA TTCCTCCTGG
    GTAAAGAGTC CTGAGAGAAC AGGCGTTAAC TTCTCAGTAA ATTCCAACTT GAGGGACCTG
    ACACCCTCAC ATCAGTTGGA GGTTGGAGGC GGCTTCCGAA TAAGTGAGTC AAAGTGCCTG
    ATGCAGGATG ATACTAGAGG CATGTTTATG GAAACACCCG TGTTTTGTAC TTCAGAAGAT
    GGGCTTGTAT CTGGTTTCGG ACGGACTGTT AATGACAATT TGATCGACGG AAGTTGCACA
    CCCCAGAATC CACCACAAAA GAAAAAGGTT TCTCTATTAG AATACCGGAA GAGACAACGT
    GAAGCTAGGA AGAGCGGCTC TAAGACGGAG CACTTTGCGC TGGTTAGTGT ATCACCTCAC
    ACAAGTGGCA ACTTGAGCAG CAGCACTGGT GATGGGTGTG CCCACAGGAG TGAAAATGGG
    GAGCAGGTAG AAAGCGCTGC TAGCCTACCT TTACCAACAC CAGCTACAGT TTATCATGCT
    ACTTCGGAAG AAACCAGCAA TAACAGTACT GTTAAGGAAG TGTCTGCCAG TGAAAAGAAT
    GAACCAGAAG TTCAATGGAC GGCCTCAACT TCAGTGGAGC AAGTGAGAGA GAGGAGTTAT
    CAGAGAGCTC TGCTTCTCAG TGATCACCGA AAAGATAAAG ACAGTGGGGC AGAGTCACCA
    TGTGTCCCCT GTTCACCGAA TCATGTTCCG TCTTCTCCTT CATCTCATTC AAATCACATT
    CCCCAGTTGC AACCCAAGGG CCCAGTCCCT TCTTTCAGTG AACTTACGGA AGATCCTGAT
    CCTGAAAATC TAGAACCCAC AACTACAAAT GAATGCCCAT CCCCAGATAC TTCCCAAAGT
    ACTTGTAAAA GTCCTTCAAA AATGAGCAAG CCTGGTTCAC CTGGACCTGT AATTCCTGTT
    CAGCCACATG GGAAAATACT TACAAAACCA GATTCCCATT GGGAGGGAAC AGTTATTGTA
    TCTGAAGCTG AGAATGGTGT CCACCTGAAA ACAGAACTCC ATCAAAAACA GCTGTCAAGT
    AACCCCCAAG CACTTTCAAA GAACCATCCT CCACAGCCAC TCGTTCGCTG TTCATCTGAG
    CAGCTTTCAC AAAAGCTGCC TTCTGCACCC ATGAAGTTGC ACTGTCCTCC GTCACCTCAC
    GTAGAAAATC CTCCAAAGTC ATCGACGCCT CACACGCCTG TACAGCATGG CTATCTCTCA
    CCAAAGCCGC CTACACAGCA GTTAGGCTCT CCCTATAGGC CTCACCATTC ACAGTCACCT
    CAAGTTGGAA CACCTCAGCG AGAGCCTCAA AGAAATTTTT ACCCAGCCGC ACAGAACCTT
    CAAGCCAGTA CTCAGCAGGC AACTTCTGGA GCATTATTTA CACAGACACC CTCAGGACAA
    TCTTCAGCAA CATACAGTCA GTTTAATCAG ACAAGTCTGA GCAGCACGGC ACCACCCCCT
    CCACCCCCTC CACCCCCTTC TTCTTCGTCT TATTATCAAA ACCAGCAGCC CTCTGCAAAC
    TTTCAGAATT ATAATCAGCT TAAAGGTAGT CTTTCCCAAC AAACTGTGTT TACATCAGGA
    CCAAATCAAG CACTTCCTGG CACCACAAGC CAGCAAACAG TTCCAGGACA CCACGTTACT
    CCAGGGCATT TTTTGCCTTC TCAGAACCCT ACTGTTCACC ATCAGACTGC TGCTGCTGTG
    GTCCCACCCC CTCCTCCACC CCCACCTGCT CCAGGACCAC ACCTCGTACA ACAGCCAAGC
    TCCCATCAGC CACACTCAGT AGCACATGTG GTGGGGCCTG TTCACGCTGT CACCCCTGGA
    TCGCATATTC ATTCTCAAAC TGCTGGGCAC CATTTGCCAC CACCGCCTCC CCCTCCTGGT
    CCTGCCCCTC ATCACCACCC CCCGCCCCAC CCTTCCACAG GACTCCAAGG TCTACAAGCA
    CAACACCAGC ATGTGGTAAA CTCAGCCCCT CCACCTCCCC CTCCTCCTCC GCCTTCCAGT
    GTTCTGGCTT CTGGGCATCA CACCACGTCA GCTCAAGCCT TACACCATCC ACCTCATCAA
    GGACCGCCAC TTTTTCCTTC AAGTGCTCAT CCAGCTGTAC CACCGTATCC CTCACAAGCT
    ACACATCACA CCACTCTGGG ACTGGGACCC CAACACCAGC CTTCTGGAAC AGGGCCACAT
    TGTCCATTAC CAGTTGCAGG TCCTCATCTC CAGCCCCAAG GACCAAACAG TATTCCAACA
    CCTACCGCTT CAGGGTTCTG TCCTCACCCT GGCTCTGTGG CCCTGCCACA TGGGGTCCAA
    GGACCTCAGC AGGCATCTCC AGTGCCTGGA CAGATTCCAA TTCACAGAGC ACAGGTGCCA
    CCGACATTTC AGAACAATTA CCATGGTTCG GGGTGGCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205424.1
    CDS253..5394
    Translation

    Target ORF information:

    RefSeq Version XM_005205367.4
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205367.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGATTG ACAGGCAGCA TATTCCTGAT ACATATCTTT GTGAACGTTG TCAGCCTAGG 
    AGTTTGGATA AAGAGAGGGC GGTGCTACTA CAACGCCGGA AAAGGGAAAA TATGTCAGAT
    GGTGATACCA GTGCAACTGA GAGTGGTGAT GAGGTTCCTG TGGAATTATA TACTGCATTT
    CAGCATACCC CAACATCGAT TACTTTAACT GCTTCAAGGG TTTCTAAGGT TAATGATAAA
    AGAAGGAAAA AAAGTGGGGA GAAAGAACAA AATATTTCAA AATGTAAAAA AGCCTTTCGT
    GAAGGATCTA GGAAATCATC AAGAGTTAAG GGCTCAGCTC CGGAGATTGA TCCTTCATCT
    GATGTTTCAA ATTTTGGATG GGAGACAAAA ATCAAAGCAT GGATGGATCA ATATGAAGAG
    GCAAATAATA ACCAATATAG TGAGAGTGTT CAGAGGGAGG CACAAAGAAT AGCTCTGAGA
    TTGGGCAATG GAAATGACAA AAAAGAGATT AAATCAGATT TGAATACCAA CAATTTGATC
    TTCAAACCTC CTGTAGAGAG CCATATACAA AAAAATAAGA AGATTCTGAA ATCTGCAAAA
    GATTTGCCTC CTGATGCACT TATCATTGAA TACAGAGGGA AGTTTATGTT GAGAGAGCAG
    TTTGAAGCAA ATGGATATTT CTTTAAGAGA CCATACCCTT TTGTTTTATT CTATTCTAAA
    TTTCATGGCC TAGAAATGTG TGTTGATGCA AGGACTTTTG GGAATGAAGC TCGGTTCATC
    AGGCGTTCTT GTACACCCAA TGCAGAGGTG AGGCATGAAA TTGAAGATGG AACCATACAT
    CTTTATATTT ATTCTATACA AAGTATTCCA AAGGGAACCG AAATTACTAT TGCCTTTGAT
    TTTGACTATG GGAATTGTAA GTACAAGGTG GATTGTGCAT GCCTCAAAGA AAATTCAGAG
    TGCCCTGTTC TGAAACGTAG TTCTGAATCC ACGGAGAACA TCAATAGCGG TTATGAGACC
    AGAAGGAAGA AAGGCAAAAA AGAAAAGGAT ATTTCAAAAG AAAAAGACAC ACAAAATCAG
    AATATTACTC TGGATTGTGA AGGAACAACG AACAAAATGA AGAGCCCAGA AACTAAACAG
    AGAAAGCTTT CTCCACTGAG ACTATCAGTA TCAAATAATC AGGAACCAGA TTTTATTGAT
    GATATAGAAG AAAAAACTCC CATCAGCAAT GAGGTAGAAA TGGAGTCTGA GGAACAGATT
    GCAGAGAGGA AAAGGAAGAT GACAAGAGAA GAAAGGAAAA TGGAAGCAAT TCTGCAGGCT
    TTTGCCAGAC TTGAAAAAAG AGAGAAGAGA AGAGAACAAG CTTTGGAAAG AATCAGCACA
    GCCAAAACTG AAGTTAAAAC TGAATGTAAA GATGCACAGA CTGTCAGTGA TGCTGAAGTT
    ATTCAGGAAC AAGCAAAAGA AGAAACTGCT AGCAAGCCAA CCCCAGCCAA AGTAAATAGA
    ACTAAACAGA GAAAAAGTTT TTCTCGGAGC AGGACTCACA TTGGACAGCA GCGTCGGAGA
    CACAGGACTG TCAGTATGTG TTCGGATATC CAACCGTCTT CTCCTGACAT CGAAGTTACC
    TCACAACAAA ACGATGTGGA AAATACTGTA CTTGCAATAG AACCAGAAAC TGAAACTGCA
    CTAACAGAAA TAATTACTGA AAATGAAGTT CCAGTACTTA ATAAATGTCC AACAAAGTAC
    CCTAAAACAA AGAAGCACTT GGTCAGTGAA TGGTTAAGTG AGAAGAATGA GAAGACAGGA
    AAACCTTCAG ACAGCCTTTC AGAGAGGCCG CTGCGCATAA CTACAGATCC TGAAGTGCTA
    GCTACACAGC TCAATTCTTT GCCAGGTCTC ACTTACAGTC CACATGTATA CTCTACTCCT
    AAGCACTATA TTAGATTCAC TTCACCATTC CTTTCGGAAA AAAGGAGAAG AAAAGAACCT
    CCTGAAAACA TATCTGGCTC ATGCAAGAAG CGGTGGTTGA AACAAGCTCT GGAAGAGGAA
    AACTCAGCAA TTTTACATAG ATTTAATTCG CCCTGTCAAG AAAGATCCAG AAGTCCTACA
    GTCAATGGTG AAAATAAAAG TCCACTACTA TTAAATGATA GCTGTTCCCT TCCAGATTTA
    ACTACACCAC TGAAAAAACG AAGACTTTAT CAGTTGCTAG ATTCTGCTTA CTCAGAAACC
    TCCACACCTA CTCCTTCACC GTATGCTACA CCAACTCACA CAGATATTAC TCCTACAGAC
    CCATCATTTT CCACTCCTCC ACGGATAAAG TCAGATGATG AAGCTTGTAG AAATGGTTAT
    AAGCCCATAT ATTCACCAGT TACCCCAGTA ACTCCTGGCA CACCAGGAAA TACCATGCAC
    TTTGAGAATA TTTCTTCCCC AGAAAGTTCT CCAGAAATTA AGAGACGCAC TTATAGTCAA
    GAGGGATATG ACAGATCGTC AACCATGTTA ACGTTGGGGC CTTTTAGAAA TTCTAACTTA
    ACTGAACTGG GTCTGCAAGA AATAAAGACT ATTGGCTATG GCAGCCCTAG GAGTAGGACT
    GAAATCAGCA GGCAGTGCCC TGGAGAAAAG GAACCGGTGT CAGATCTTCA ACTGGGACTC
    GATGCAGTCG AGCAGACCGC CTTACATAAA ACCATGGAAG CAGCTGCACA CGACAGGACT
    GAGGCTGCCA GCCAGCTGGA GGCTGCTCAC TCGGGGCGGG GCCCTCTGTA TTCCTCCTGG
    GTAAAGAGTC CTGAGAGAAC AGGCGTTAAC TTCTCAGTAA ATTCCAACTT GAGGGACCTG
    ACACCCTCAC ATCAGTTGGA GGTTGGAGGC GGCTTCCGAA TAAGTGAGTC AAAGTGCCTG
    ATGCAGGATG ATACTAGAGG CATGTTTATG GAAACACCCG TGTTTTGTAC TTCAGAAGAT
    GGGCTTGTAT CTGGTTTCGG ACGGACTGTT AATGACAATT TGATCGACGG AAGTTGCACA
    CCCCAGAATC CACCACAAAA GAAAAAGGTT TCTCTATTAG AATACCGGAA GAGACAACGT
    GAAGCTAGGA AGAGCGGCTC TAAGACGGAG CACTTTGCGC TGGTTAGTGT ATCACCTCAC
    ACAAGTGGCA ACTTGAGCAG CAGCACTGGT GATGGGTGTG CCCACAGGAG TGAAAATGGG
    GAGCAGGTAG AAAGCGCTGC TAGCCTACCT TTACCAACAC CAGCTACAGT TTATCATGCT
    ACTTCGGAAG AAACCAGCAA TAACAGTACT GTTAAGGAAG TGTCTGCCAG TGAAAAGAAT
    GAACCAGAAG TTCAATGGAC GGCCTCAACT TCAGTGGAGC AAGTGAGAGA GAGGAGTTAT
    CAGAGAGCTC TGCTTCTCAG TGATCACCGA AAAGATAAAG ACAGTGGGGC AGAGTCACCA
    TGTGTCCCCT GTTCACCGAA TCATGTTCCG TCTTCTCCTT CATCTCATTC AAATCACATT
    CCCCAGTTGC AACCCAAGGG CCCAGTCCCT TCTTTCAGTG AACTTACGGA AGATCCTGAT
    CCTGAAAATC TAGAACCCAC AACTACAAAT GAATGCCCAT CCCCAGATAC TTCCCAAAGT
    ACTTGTAAAA GTCCTTCAAA AATGAGCAAG CCTGGTTCAC CTGGACCTGT AATTCCTGTT
    CAGCCACATG GGAAAATACT TACAAAACCA GATTCCCATT GGGAGGGAAC AGTTATTGTA
    TCTGAAGCTG AGAATGGTGT CCACCTGAAA ACAGAACTCC ATCAAAAACA GCTGTCAAGT
    AACCCCCAAG CACTTTCAAA GAACCATCCT CCACAGCCAC TCGTTCGCTG TTCATCTGAG
    CAGCTTTCAC AAAAGCTGCC TTCTGCACCC ATGAAGTTGC ACTGTCCTCC GTCACCTCAC
    GTAGAAAATC CTCCAAAGTC ATCGACGCCT CACACGCCTG TACAGCATGG CTATCTCTCA
    CCAAAGCCGC CTACACAGCA GTTAGGCTCT CCCTATAGGC CTCACCATTC ACAGTCACCT
    CAAGTTGGAA CACCTCAGCG AGAGCCTCAA AGAAATTTTT ACCCAGCCGC ACAGAACCTT
    CAAGCCAGTA CTCAGCAGGC AACTTCTGGA GCATTATTTA CACAGACACC CTCAGGACAA
    TCTTCAGCAA CATACAGTCA GTTTAATCAG ACAAGTCTGA GCAGCACGGC ACCACCCCCT
    CCACCCCCTC CACCCCCTTC TTCTTCGTCT TATTATCAAA ACCAGCAGCC CTCTGCAAAC
    TTTCAGAATT ATAATCAGCT TAAAGGTAGT CTTTCCCAAC AAACTGTGTT TACATCAGGA
    CCAAATCAAG CACTTCCTGG CACCACAAGC CAGCAAACAG TTCCAGGACA CCACGTTACT
    CCAGGGCATT TTTTGCCTTC TCAGAACCCT ACTGTTCACC ATCAGACTGC TGCTGCTGTG
    GTCCCACCCC CTCCTCCACC CCCACCTGCT CCAGGACCAC ACCTCGTACA ACAGCCAAGC
    TCCCATCAGC CACACTCAGT AGCACATGTG GTGGGGCCTG TTCACGCTGT CACCCCTGGA
    TCGCATATTC ATTCTCAAAC TGCTGGGCAC CATTTGCCAC CACCGCCTCC CCCTCCTGGT
    CCTGCCCCTC ATCACCACCC CCCGCCCCAC CCTTCCACAG GACTCCAAGG TCTACAAGCA
    CAACACCAGC ATGTGGTAAA CTCAGCCCCT CCACCTCCCC CTCCTCCTCC GCCTTCCAGT
    GTTCTGGCTT CTGGGCATCA CACCACGTCA GCTCAAGCCT TACACCATCC ACCTCATCAA
    GGACCGCCAC TTTTTCCTTC AAGTGCTCAT CCAGCTGTAC CACCGTATCC CTCACAAGCT
    ACACATCACA CCACTCTGGG ACTGGGACCC CAACACCAGC CTTCTGGAAC AGGGCCACAT
    TGTCCATTAC CAGTTGCAGG TCCTCATCTC CAGCCCCAAG GACCAAACAG TATTCCAACA
    CCTACCGCTT CAGGGTTCTG TCCTCACCCT GGCTCTGTGG CCCTGCCACA TGGGGTCCAA
    GGACCTCAGC AGGCATCTCC AGTGCCTGGA CAGATTCCAA TTCACAGAGC ACAGGTGCCA
    CCGACATTTC AGAACAATTA CCATGGTTCG GGGTGGCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233779
    Clone ID Related Accession (Same CDS sequence) XM_024990819.1
    Accession Version XM_024990819.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive histone-lysine N-methyltransferase 2E isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGATTG ACAGGCAGCA TATTCCTGAT ACATATCTTT GTGAACGTTG TCAGCCTAGG 
    AGTTTGGATA AAGAGAGGGC GGTGCTACTA CAACGCCGGA AAAGGGAAAA TATGTCAGAT
    GGTGATACCA GTGCAACTGA GAGTGGTGAT GAGGTTCCTG TGGAATTATA TACTGCATTT
    CAGCATACCC CAACATCGAT TACTTTAACT GCTTCAAGGG TTTCTAAGGT TAATGATAAA
    AGAAGGAAAA AAAGTGGGGA GAAAGAACAA AATATTTCAA AATGTAAAAA AGCCTTTCGT
    GAAGGATCTA GGAAATCATC AAGAGTTAAG GGCTCAGCTC CGGAGATTGA TCCTTCATCT
    GATGTTTCAA ATTTTGGATG GGAGACAAAA ATCAAAGCAT GGATGGATCA ATATGAAGAG
    GCAAATAATA ACCAATATAG TGAGAGTGTT CAGAGGGAGG CACAAAGAAT AGCTCTGAGA
    TTGGGCAATG GAAATGACAA AAAAGAGATT AAATCAGATT TGAATACCAA CAATTTGATC
    TTCAAACCTC CTGTAGAGAG CCATATACAA AAAAATAAGA AGATTCTGAA ATCTGCAAAA
    GATTTGCCTC CTGATGCACT TATCATTGAA TACAGAGGGA AGTTTATGTT GAGAGAGCAG
    TTTGAAGCAA ATGGATATTT CTTTAAGAGA CCATACCCTT TTGTTTTATT CTATTCTAAA
    TTTCATGGCC TAGAAATGTG TGTTGATGCA AGGACTTTTG GGAATGAAGC TCGGTTCATC
    AGGCGTTCTT GTACACCCAA TGCAGAGGTG AGGCATGAAA TTGAAGATGG AACCATACAT
    CTTTATATTT ATTCTATACA AAGTATTCCA AAGGGAACCG AAATTACTAT TGCCTTTGAT
    TTTGACTATG GGAATTGTAA GTACAAGGTG GATTGTGCAT GCCTCAAAGA AAATTCAGAG
    TGCCCTGTTC TGAAACGTAG TTCTGAATCC ACGGAGAACA TCAATAGCGG TTATGAGACC
    AGAAGGAAGA AAGGCAAAAA AGAAAAGGAT ATTTCAAAAG AAAAAGACAC ACAAAATCAG
    AATATTACTC TGGATTGTGA AGGAACAACG AACAAAATGA AGAGCCCAGA AACTAAACAG
    AGAAAGCTTT CTCCACTGAG ACTATCAGTA TCAAATAATC AGGAACCAGA TTTTATTGAT
    GATATAGAAG AAAAAACTCC CATCAGCAAT GAGGTAGAAA TGGAGTCTGA GGAACAGATT
    GCAGAGAGGA AAAGGAAGAT GACAAGAGAA GAAAGGAAAA TGGAAGCAAT TCTGCAGGCT
    TTTGCCAGAC TTGAAAAAAG AGAGAAGAGA AGAGAACAAG CTTTGGAAAG AATCAGCACA
    GCCAAAACTG AAGTTAAAAC TGAATGTAAA GATGCACAGA CTGTCAGTGA TGCTGAAGTT
    ATTCAGGAAC AAGCAAAAGA AGAAACTGCT AGCAAGCCAA CCCCAGCCAA AGTAAATAGA
    ACTAAACAGA GAAAAAGTTT TTCTCGGAGC AGGACTCACA TTGGACAGCA GCGTCGGAGA
    CACAGGACTG TCAGTATGTG TTCGGATATC CAACCGTCTT CTCCTGACAT CGAAGTTACC
    TCACAACAAA ACGATGTGGA AAATACTGTA CTTGCAATAG AACCAGAAAC TGAAACTGCA
    CTAACAGAAA TAATTACTGA AAATGAAGTT CCAGTACTTA ATAAATGTCC AACAAAGTAC
    CCTAAAACAA AGAAGCACTT GGTCAGTGAA TGGTTAAGTG AGAAGAATGA GAAGACAGGA
    AAACCTTCAG ACAGCCTTTC AGAGAGGCCG CTGCGCATAA CTACAGATCC TGAAGTGCTA
    GCTACACAGC TCAATTCTTT GCCAGGTCTC ACTTACAGTC CACATGTATA CTCTACTCCT
    AAGCACTATA TTAGATTCAC TTCACCATTC CTTTCGGAAA AAAGGAGAAG AAAAGAACCT
    CCTGAAAACA TATCTGGCTC ATGCAAGAAG CGGTGGTTGA AACAAGCTCT GGAAGAGGAA
    AACTCAGCAA TTTTACATAG ATTTAATTCG CCCTGTCAAG AAAGATCCAG AAGTCCTACA
    GTCAATGGTG AAAATAAAAG TCCACTACTA TTAAATGATA GCTGTTCCCT TCCAGATTTA
    ACTACACCAC TGAAAAAACG AAGACTTTAT CAGTTGCTAG ATTCTGCTTA CTCAGAAACC
    TCCACACCTA CTCCTTCACC GTATGCTACA CCAACTCACA CAGATATTAC TCCTACAGAC
    CCATCATTTT CCACTCCTCC ACGGATAAAG TCAGATGATG AAGCTTGTAG AAATGGTTAT
    AAGCCCATAT ATTCACCAGT TACCCCAGTA ACTCCTGGCA CACCAGGAAA TACCATGCAC
    TTTGAGAATA TTTCTTCCCC AGAAAGTTCT CCAGAAATTA AGAGACGCAC TTATAGTCAA
    GAGGGATATG ACAGATCGTC AACCATGTTA ACGTTGGGGC CTTTTAGAAA TTCTAACTTA
    ACTGAACTGG GTCTGCAAGA AATAAAGACT ATTGGCTATG GCAGCCCTAG GAGTAGGACT
    GAAATCAGCA GGCAGTGCCC TGGAGAAAAG GAACCGGTGT CAGATCTTCA ACTGGGACTC
    GATGCAGTCG AGCAGACCGC CTTACATAAA ACCATGGAAG CAGCTGCACA CGACAGGACT
    GAGGCTGCCA GCCAGCTGGA GGCTGCTCAC TCGGGGCGGG GCCCTCTGTA TTCCTCCTGG
    GTAAAGAGTC CTGAGAGAAC AGGCGTTAAC TTCTCAGTAA ATTCCAACTT GAGGGACCTG
    ACACCCTCAC ATCAGTTGGA GGTTGGAGGC GGCTTCCGAA TAAGTGAGTC AAAGTGCCTG
    ATGCAGGATG ATACTAGAGG CATGTTTATG GAAACACCCG TGTTTTGTAC TTCAGAAGAT
    GGGCTTGTAT CTGGTTTCGG ACGGACTGTT AATGACAATT TGATCGACGG AAGTTGCACA
    CCCCAGAATC CACCACAAAA GAAAAAGGTT TCTCTATTAG AATACCGGAA GAGACAACGT
    GAAGCTAGGA AGAGCGGCTC TAAGACGGAG CACTTTGCGC TGGTTAGTGT ATCACCTCAC
    ACAAGTGGCA ACTTGAGCAG CAGCACTGGT GATGGGTGTG CCCACAGGAG TGAAAATGGG
    GAGCAGGTAG AAAGCGCTGC TAGCCTACCT TTACCAACAC CAGCTACAGT TTATCATGCT
    ACTTCGGAAG AAACCAGCAA TAACAGTACT GTTAAGGAAG TGTCTGCCAG TGAAAAGAAT
    GAACCAGAAG TTCAATGGAC GGCCTCAACT TCAGTGGAGC AAGTGAGAGA GAGGAGTTAT
    CAGAGAGCTC TGCTTCTCAG TGATCACCGA AAAGATAAAG ACAGTGATCC TGATCCTGAA
    AATCTAGAAC CCACAACTAC AAATGAATGC CCATCCCCAG ATACTTCCCA AAGTACTTGT
    AAAAGTCCTT CAAAAATGAG CAAGCCTGGT TCACCTGGAC CTGTAATTCC TGTTCAGCCA
    CATGGGAAAA TACTTACAAA ACCAGATTCC CATTGGGAGG GAACAGTTAT TGTATCTGAA
    GCTGAGAATG GTGTCCACCT GAAAACAGAA CTCCATCAAA AACAGCTGTC AAGTAACCCC
    CAAGCACTTT CAAAGAACCA TCCTCCACAG CCACTCGTTC GCTGTTCATC TGAGCAGCTT
    TCACAAAAGC TGCCTTCTGC ACCCATGAAG TTGCACTGTC CTCCGTCACC TCACGTAGAA
    AATCCTCCAA AGTCATCGAC GCCTCACACG CCTGTACAGC ATGGCTATCT CTCACCAAAG
    CCGCCTACAC AGCAGTTAGG CTCTCCCTAT AGGCCTCACC ATTCACAGTC ACCTCAAGTT
    GGAACACCTC AGCGAGAGCC TCAAAGAAAT TTTTACCCAG CCGCACAGAA CCTTCAAGCC
    AGTACTCAGC AGGCAACTTC TGGAGCATTA TTTACACAGA CACCCTCAGG ACAATCTTCA
    GCAACATACA GTCAGTTTAA TCAGACAAGT CTGAGCAGCA CGGCACCACC CCCTCCACCC
    CCTCCACCCC CTTCTTCTTC GTCTTATTAT CAAAACCAGC AGCCCTCTGC AAACTTTCAG
    AATTATAATC AGCTTAAAGG TAGTCTTTCC CAACAAACTG TGTTTACATC AGGACCAAAT
    CAAGCACTTC CTGGCACCAC AAGCCAGCAA ACAGTTCCAG GACACCACGT TACTCCAGGG
    CATTTTTTGC CTTCTCAGAA CCCTACTGTT CACCATCAGA CTGCTGCTGC TGTGGTCCCA
    CCCCCTCCTC CACCCCCACC TGCTCCAGGA CCACACCTCG TACAACAGCC AAGCTCCCAT
    CAGCCACACT CAGTAGCACA TGTGGTGGGG CCTGTTCACG CTGTCACCCC TGGATCGCAT
    ATTCATTCTC AAACTGCTGG GCACCATTTG CCACCACCGC CTCCCCCTCC TGGTCCTGCC
    CCTCATCACC ACCCCCCGCC CCACCCTTCC ACAGGACTCC AAGGTCTACA AGCACAACAC
    CAGCATGTGG TAAACTCAGC CCCTCCACCT CCCCCTCCTC CTCCGCCTTC CAGTGTTCTG
    GCTTCTGGGC ATCACACCAC GTCAGCTCAA GCCTTACACC ATCCACCTCA TCAAGGACCG
    CCACTTTTTC CTTCAAGTGC TCATCCAGCT GTACCACCGT ATCCCTCACA AGCTACACAT
    CACACCACTC TGGGACTGGG ACCCCAACAC CAGCCTTCTG GAACAGGGCC ACATTGTCCA
    TTACCAGTTG CAGGTCCTCA TCTCCAGCCC CAAGGACCAA ACAGTATTCC AACACCTACC
    GCTTCAGGGT TCTGTCCTCA CCCTGGCTCT GTGGCCCTGC CACATGGGGT CCAAGGACCT
    CAGCAGGCAT CTCCAGTGCC TGGACAGATT CCAATTCACA GAGCACAGGT GCCACCGACA
    TTTCAGAACA ATTACCATGG TTCGGGGTGG CATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846587.1
    CDS998..6013
    Translation

    Target ORF information:

    RefSeq Version XM_024990819.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990819.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGATTG ACAGGCAGCA TATTCCTGAT ACATATCTTT GTGAACGTTG TCAGCCTAGG 
    AGTTTGGATA AAGAGAGGGC GGTGCTACTA CAACGCCGGA AAAGGGAAAA TATGTCAGAT
    GGTGATACCA GTGCAACTGA GAGTGGTGAT GAGGTTCCTG TGGAATTATA TACTGCATTT
    CAGCATACCC CAACATCGAT TACTTTAACT GCTTCAAGGG TTTCTAAGGT TAATGATAAA
    AGAAGGAAAA AAAGTGGGGA GAAAGAACAA AATATTTCAA AATGTAAAAA AGCCTTTCGT
    GAAGGATCTA GGAAATCATC AAGAGTTAAG GGCTCAGCTC CGGAGATTGA TCCTTCATCT
    GATGTTTCAA ATTTTGGATG GGAGACAAAA ATCAAAGCAT GGATGGATCA ATATGAAGAG
    GCAAATAATA ACCAATATAG TGAGAGTGTT CAGAGGGAGG CACAAAGAAT AGCTCTGAGA
    TTGGGCAATG GAAATGACAA AAAAGAGATT AAATCAGATT TGAATACCAA CAATTTGATC
    TTCAAACCTC CTGTAGAGAG CCATATACAA AAAAATAAGA AGATTCTGAA ATCTGCAAAA
    GATTTGCCTC CTGATGCACT TATCATTGAA TACAGAGGGA AGTTTATGTT GAGAGAGCAG
    TTTGAAGCAA ATGGATATTT CTTTAAGAGA CCATACCCTT TTGTTTTATT CTATTCTAAA
    TTTCATGGCC TAGAAATGTG TGTTGATGCA AGGACTTTTG GGAATGAAGC TCGGTTCATC
    AGGCGTTCTT GTACACCCAA TGCAGAGGTG AGGCATGAAA TTGAAGATGG AACCATACAT
    CTTTATATTT ATTCTATACA AAGTATTCCA AAGGGAACCG AAATTACTAT TGCCTTTGAT
    TTTGACTATG GGAATTGTAA GTACAAGGTG GATTGTGCAT GCCTCAAAGA AAATTCAGAG
    TGCCCTGTTC TGAAACGTAG TTCTGAATCC ACGGAGAACA TCAATAGCGG TTATGAGACC
    AGAAGGAAGA AAGGCAAAAA AGAAAAGGAT ATTTCAAAAG AAAAAGACAC ACAAAATCAG
    AATATTACTC TGGATTGTGA AGGAACAACG AACAAAATGA AGAGCCCAGA AACTAAACAG
    AGAAAGCTTT CTCCACTGAG ACTATCAGTA TCAAATAATC AGGAACCAGA TTTTATTGAT
    GATATAGAAG AAAAAACTCC CATCAGCAAT GAGGTAGAAA TGGAGTCTGA GGAACAGATT
    GCAGAGAGGA AAAGGAAGAT GACAAGAGAA GAAAGGAAAA TGGAAGCAAT TCTGCAGGCT
    TTTGCCAGAC TTGAAAAAAG AGAGAAGAGA AGAGAACAAG CTTTGGAAAG AATCAGCACA
    GCCAAAACTG AAGTTAAAAC TGAATGTAAA GATGCACAGA CTGTCAGTGA TGCTGAAGTT
    ATTCAGGAAC AAGCAAAAGA AGAAACTGCT AGCAAGCCAA CCCCAGCCAA AGTAAATAGA
    ACTAAACAGA GAAAAAGTTT TTCTCGGAGC AGGACTCACA TTGGACAGCA GCGTCGGAGA
    CACAGGACTG TCAGTATGTG TTCGGATATC CAACCGTCTT CTCCTGACAT CGAAGTTACC
    TCACAACAAA ACGATGTGGA AAATACTGTA CTTGCAATAG AACCAGAAAC TGAAACTGCA
    CTAACAGAAA TAATTACTGA AAATGAAGTT CCAGTACTTA ATAAATGTCC AACAAAGTAC
    CCTAAAACAA AGAAGCACTT GGTCAGTGAA TGGTTAAGTG AGAAGAATGA GAAGACAGGA
    AAACCTTCAG ACAGCCTTTC AGAGAGGCCG CTGCGCATAA CTACAGATCC TGAAGTGCTA
    GCTACACAGC TCAATTCTTT GCCAGGTCTC ACTTACAGTC CACATGTATA CTCTACTCCT
    AAGCACTATA TTAGATTCAC TTCACCATTC CTTTCGGAAA AAAGGAGAAG AAAAGAACCT
    CCTGAAAACA TATCTGGCTC ATGCAAGAAG CGGTGGTTGA AACAAGCTCT GGAAGAGGAA
    AACTCAGCAA TTTTACATAG ATTTAATTCG CCCTGTCAAG AAAGATCCAG AAGTCCTACA
    GTCAATGGTG AAAATAAAAG TCCACTACTA TTAAATGATA GCTGTTCCCT TCCAGATTTA
    ACTACACCAC TGAAAAAACG AAGACTTTAT CAGTTGCTAG ATTCTGCTTA CTCAGAAACC
    TCCACACCTA CTCCTTCACC GTATGCTACA CCAACTCACA CAGATATTAC TCCTACAGAC
    CCATCATTTT CCACTCCTCC ACGGATAAAG TCAGATGATG AAGCTTGTAG AAATGGTTAT
    AAGCCCATAT ATTCACCAGT TACCCCAGTA ACTCCTGGCA CACCAGGAAA TACCATGCAC
    TTTGAGAATA TTTCTTCCCC AGAAAGTTCT CCAGAAATTA AGAGACGCAC TTATAGTCAA
    GAGGGATATG ACAGATCGTC AACCATGTTA ACGTTGGGGC CTTTTAGAAA TTCTAACTTA
    ACTGAACTGG GTCTGCAAGA AATAAAGACT ATTGGCTATG GCAGCCCTAG GAGTAGGACT
    GAAATCAGCA GGCAGTGCCC TGGAGAAAAG GAACCGGTGT CAGATCTTCA ACTGGGACTC
    GATGCAGTCG AGCAGACCGC CTTACATAAA ACCATGGAAG CAGCTGCACA CGACAGGACT
    GAGGCTGCCA GCCAGCTGGA GGCTGCTCAC TCGGGGCGGG GCCCTCTGTA TTCCTCCTGG
    GTAAAGAGTC CTGAGAGAAC AGGCGTTAAC TTCTCAGTAA ATTCCAACTT GAGGGACCTG
    ACACCCTCAC ATCAGTTGGA GGTTGGAGGC GGCTTCCGAA TAAGTGAGTC AAAGTGCCTG
    ATGCAGGATG ATACTAGAGG CATGTTTATG GAAACACCCG TGTTTTGTAC TTCAGAAGAT
    GGGCTTGTAT CTGGTTTCGG ACGGACTGTT AATGACAATT TGATCGACGG AAGTTGCACA
    CCCCAGAATC CACCACAAAA GAAAAAGGTT TCTCTATTAG AATACCGGAA GAGACAACGT
    GAAGCTAGGA AGAGCGGCTC TAAGACGGAG CACTTTGCGC TGGTTAGTGT ATCACCTCAC
    ACAAGTGGCA ACTTGAGCAG CAGCACTGGT GATGGGTGTG CCCACAGGAG TGAAAATGGG
    GAGCAGGTAG AAAGCGCTGC TAGCCTACCT TTACCAACAC CAGCTACAGT TTATCATGCT
    ACTTCGGAAG AAACCAGCAA TAACAGTACT GTTAAGGAAG TGTCTGCCAG TGAAAAGAAT
    GAACCAGAAG TTCAATGGAC GGCCTCAACT TCAGTGGAGC AAGTGAGAGA GAGGAGTTAT
    CAGAGAGCTC TGCTTCTCAG TGATCACCGA AAAGATAAAG ACAGTGATCC TGATCCTGAA
    AATCTAGAAC CCACAACTAC AAATGAATGC CCATCCCCAG ATACTTCCCA AAGTACTTGT
    AAAAGTCCTT CAAAAATGAG CAAGCCTGGT TCACCTGGAC CTGTAATTCC TGTTCAGCCA
    CATGGGAAAA TACTTACAAA ACCAGATTCC CATTGGGAGG GAACAGTTAT TGTATCTGAA
    GCTGAGAATG GTGTCCACCT GAAAACAGAA CTCCATCAAA AACAGCTGTC AAGTAACCCC
    CAAGCACTTT CAAAGAACCA TCCTCCACAG CCACTCGTTC GCTGTTCATC TGAGCAGCTT
    TCACAAAAGC TGCCTTCTGC ACCCATGAAG TTGCACTGTC CTCCGTCACC TCACGTAGAA
    AATCCTCCAA AGTCATCGAC GCCTCACACG CCTGTACAGC ATGGCTATCT CTCACCAAAG
    CCGCCTACAC AGCAGTTAGG CTCTCCCTAT AGGCCTCACC ATTCACAGTC ACCTCAAGTT
    GGAACACCTC AGCGAGAGCC TCAAAGAAAT TTTTACCCAG CCGCACAGAA CCTTCAAGCC
    AGTACTCAGC AGGCAACTTC TGGAGCATTA TTTACACAGA CACCCTCAGG ACAATCTTCA
    GCAACATACA GTCAGTTTAA TCAGACAAGT CTGAGCAGCA CGGCACCACC CCCTCCACCC
    CCTCCACCCC CTTCTTCTTC GTCTTATTAT CAAAACCAGC AGCCCTCTGC AAACTTTCAG
    AATTATAATC AGCTTAAAGG TAGTCTTTCC CAACAAACTG TGTTTACATC AGGACCAAAT
    CAAGCACTTC CTGGCACCAC AAGCCAGCAA ACAGTTCCAG GACACCACGT TACTCCAGGG
    CATTTTTTGC CTTCTCAGAA CCCTACTGTT CACCATCAGA CTGCTGCTGC TGTGGTCCCA
    CCCCCTCCTC CACCCCCACC TGCTCCAGGA CCACACCTCG TACAACAGCC AAGCTCCCAT
    CAGCCACACT CAGTAGCACA TGTGGTGGGG CCTGTTCACG CTGTCACCCC TGGATCGCAT
    ATTCATTCTC AAACTGCTGG GCACCATTTG CCACCACCGC CTCCCCCTCC TGGTCCTGCC
    CCTCATCACC ACCCCCCGCC CCACCCTTCC ACAGGACTCC AAGGTCTACA AGCACAACAC
    CAGCATGTGG TAAACTCAGC CCCTCCACCT CCCCCTCCTC CTCCGCCTTC CAGTGTTCTG
    GCTTCTGGGC ATCACACCAC GTCAGCTCAA GCCTTACACC ATCCACCTCA TCAAGGACCG
    CCACTTTTTC CTTCAAGTGC TCATCCAGCT GTACCACCGT ATCCCTCACA AGCTACACAT
    CACACCACTC TGGGACTGGG ACCCCAACAC CAGCCTTCTG GAACAGGGCC ACATTGTCCA
    TTACCAGTTG CAGGTCCTCA TCTCCAGCCC CAAGGACCAA ACAGTATTCC AACACCTACC
    GCTTCAGGGT TCTGTCCTCA CCCTGGCTCT GTGGCCCTGC CACATGGGGT CCAAGGACCT
    CAGCAGGCAT CTCCAGTGCC TGGACAGATT CCAATTCACA GAGCACAGGT GCCACCGACA
    TTTCAGAACA ATTACCATGG TTCGGGGTGG CATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01901
    Clone ID Related Accession (Same CDS sequence) NM_001082451.2 , XM_005205363.4 , XM_005205365.4 , XM_005205362.4 , NM_001082451.3 , XM_005205364.4
    Accession Version NM_001082451.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-12-31
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase 2E
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03120932.1. On Dec 23, 2009 this sequence version replaced NM_001082451.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001075920.2
    CDS381..5960
    Translation

    Target ORF information:

    RefSeq Version NM_001082451.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001082451.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01901
    Clone ID Related Accession (Same CDS sequence) NM_001082451.2 , XM_005205363.4 , XM_005205365.4 , XM_005205362.4 , NM_001082451.3 , XM_005205364.4
    Accession Version NM_001082451.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-15
    Organism Bos taurus(cattle)
    Product inactive histone-lysine N-methyltransferase 2E
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000004.1. On Jun 16, 2018 this sequence version replaced NM_001082451.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR6764281.15648.1, SRR5571260.45118.1 [ECO:0000331] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001075920.2
    CDS381..5960
    Translation

    Target ORF information:

    RefSeq Version NM_001082451.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine methyltransferase 2E (KMT2E), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001082451.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01901
    Clone ID Related Accession (Same CDS sequence) NM_001082451.2 , XM_005205363.4 , XM_005205365.4 , XM_005205362.4 , NM_001082451.3 , XM_005205364.4
    Accession Version XM_005205363.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive histone-lysine N-methyltransferase 2E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205363.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205420.1
    CDS554..6133
    Translation

    Target ORF information:

    RefSeq Version XM_005205363.4
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205363.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01901
    Clone ID Related Accession (Same CDS sequence) NM_001082451.2 , XM_005205363.4 , XM_005205365.4 , XM_005205362.4 , NM_001082451.3 , XM_005205364.4
    Accession Version XM_005205365.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive histone-lysine N-methyltransferase 2E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205365.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205422.1
    CDS176..5755
    Translation

    Target ORF information:

    RefSeq Version XM_005205365.4
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205365.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01901
    Clone ID Related Accession (Same CDS sequence) NM_001082451.2 , XM_005205363.4 , XM_005205365.4 , XM_005205362.4 , NM_001082451.3 , XM_005205364.4
    Accession Version XM_005205362.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive histone-lysine N-methyltransferase 2E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205362.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205419.1
    CDS510..6089
    Translation

    Target ORF information:

    RefSeq Version XM_005205362.4
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205362.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01901
    Clone ID Related Accession (Same CDS sequence) NM_001082451.2 , XM_005205363.4 , XM_005205365.4 , XM_005205362.4 , NM_001082451.3 , XM_005205364.4
    Accession Version XM_005205364.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inactive histone-lysine N-methyltransferase 2E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205364.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205421.1
    CDS613..6192
    Translation

    Target ORF information:

    RefSeq Version XM_005205364.4
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine methyltransferase 2E (KMT2E), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205364.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCGCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18519
    Clone ID Related Accession (Same CDS sequence) XM_005205362.3 , XM_005205363.3 , XM_005205364.3 , XM_005205365.3
    Accession Version XM_005205362.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase 2E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205362.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205419.1
    CDS450..6029
    Translation

    Target ORF information:

    RefSeq Version XM_005205362.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205362.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18519
    Clone ID Related Accession (Same CDS sequence) XM_005205362.3 , XM_005205363.3 , XM_005205364.3 , XM_005205365.3
    Accession Version XM_005205363.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase 2E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205363.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205420.1
    CDS523..6102
    Translation

    Target ORF information:

    RefSeq Version XM_005205363.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205363.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18519
    Clone ID Related Accession (Same CDS sequence) XM_005205362.3 , XM_005205363.3 , XM_005205364.3 , XM_005205365.3
    Accession Version XM_005205364.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase 2E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205364.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205421.1
    CDS592..6171
    Translation

    Target ORF information:

    RefSeq Version XM_005205364.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205364.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18519
    Clone ID Related Accession (Same CDS sequence) XM_005205362.3 , XM_005205363.3 , XM_005205364.3 , XM_005205365.3
    Accession Version XM_005205365.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase 2E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205365.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205422.1
    CDS144..5723
    Translation

    Target ORF information:

    RefSeq Version XM_005205365.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205365.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGGGGCAG AGTCACCATG TGTCCCCTGT TCACCGAATC ATGTTCCGTC TTCTCCTTCA
    TCTCATTCAA ATCACATTCC CCAGTTGCAA CCCAAGGGCC CAGTCCCTTC TTTCAGTGAA
    CTTACGGAAG ATCCTGATCC TGAAAATCTA GAACCCACAA CTACAAATGA ATGCCCATCC
    CCAGATACTT CCCAAAGTAC TTGTAAAAGT CCTTCAAAAA TGAGCAAGCC TGGTTCACCT
    GGACCTGTAA TTCCTGTTCA GCCACATGGG AAAATACTTA CAAAACCAGA TTCCCATTGG
    GAGGGAACAG TTATTGTATC TGAAGCTGAG AATGGTGTCC ACCTGAAAAC AGAACTCCAT
    CAAAAACAGC TGTCAAGTAA CCCCCAAGCA CTTTCAAAGA ACCATCCTCC ACAGCCACTC
    GTTCGCTGTT CATCTGAGCA GCTTTCACAA AAGCTGCCTT CTGCACCCAT GAAGTTGCAC
    TGTCCTCCGT CACCTCACGT AGAAAATCCT CCAAAGTCAT CGACGCCTCA CACGCCTGTA
    CAGCATGGCT ATCTCTCACC AAAGCCTCCT ACACAGCAGT TAGGCTCTCC CTATAGGCCT
    CACCATTCAC AGTCACCTCA AGTTGGAACA CCTCAGCGAG AGCCTCAAAG AAATTTTTAC
    CCAGCCGCAC AGAACCTTCA AGCCAGTACT CAGCAGGCAA CTTCTGGAGC ATTATTTACA
    CAGACACCCT CAGGACAATC TTCAGCAACA TACAGTCAGT TTAATCAGAC AAGTCTGAGC
    AGCACGGCAC CACCCCCTCC ACCCCCTCCA CCCCCTTCTT CTTCGTCTTA TTATCAAAAC
    CAGCAGCCCT CTGCAAACTT TCAGAATTAT AATCAGCTTA AAGGTAGTCT TTCCCAACAA
    ACTGTGTTTA CATCAGGACC AAATCAAGCA CTTCCTGGCA CCACAAGCCA GCAAACAGTT
    CCAGGACACC ACGTTACTCC AGGGCATTTT TTGCCTTCTC AGAACCCTAC TGTTCACCAT
    CAGACTGCTG CTGCTGTGGT CCCACCCCCT CCTCCACCCC CACCTGCTCC AGGACCACAC
    CTCGTACAAC AGCCAAGCTC CCATCAGCCA CACTCAGTAG CACATGTGGT GGGGCCTGTT
    CACGCTGTCA CCCCTGGATC GCATATTCAT TCTCAAACTG CTGGGCACCA TTTGCCACCA
    CCGCCTCCCC CTCCTGGTCC TGCCCCTCAT CACCACCCCC CGCCCCACCC TTCCACAGGA
    CTCCAAGGTC TACAAGCACA ACACCAGCAT GTGGTAAACT CAGCCCCTCC ACCTCCCCCT
    CCTCCTCCGC CTTCCAGTGT TCTGGCTTCT GGGCATCACA CCACGTCAGC TCAAGCCTTA
    CACCATCCAC CTCATCAAGG ACCGCCACTT TTTCCTTCAA GTGCTCATCC AGCTGTACCA
    CCGTATCCCT CACAAGCTAC ACATCACACC ACTCTGGGAC TGGGACCCCA ACACCAGCCT
    TCTGGAACAG GGCCACATTG TCCATTACCA GTTGCAGGTC CTCATCTCCA GCCCCAAGGA
    CCAAACAGTA TTCCAACACC TACCGCTTCA GGGTTCTGTC CTCACCCTGG CTCTGTGGCC
    CTGCCACATG GGGTCCAAGG ACCTCAGCAG GCATCTCCAG TGCCTGGACA GATTCCAATT
    CACAGAGCAC AGGTGCCACC GACATTTCAG AACAATTACC ATGGTTCGGG GTGGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18520
    Clone ID Related Accession (Same CDS sequence) XM_005205366.3
    Accession Version XM_005205366.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5454bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase 2E isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205366.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGATCCTG ATCCTGAAAA TCTAGAACCC ACAACTACAA ATGAATGCCC ATCCCCAGAT
    ACTTCCCAAA GTACTTGTAA AAGTCCTTCA AAAATGAGCA AGCCTGGTTC ACCTGGACCT
    GTAATTCCTG TTCAGCCACA TGGGAAAATA CTTACAAAAC CAGATTCCCA TTGGGAGGGA
    ACAGTTATTG TATCTGAAGC TGAGAATGGT GTCCACCTGA AAACAGAACT CCATCAAAAA
    CAGCTGTCAA GTAACCCCCA AGCACTTTCA AAGAACCATC CTCCACAGCC ACTCGTTCGC
    TGTTCATCTG AGCAGCTTTC ACAAAAGCTG CCTTCTGCAC CCATGAAGTT GCACTGTCCT
    CCGTCACCTC ACGTAGAAAA TCCTCCAAAG TCATCGACGC CTCACACGCC TGTACAGCAT
    GGCTATCTCT CACCAAAGCC TCCTACACAG CAGTTAGGCT CTCCCTATAG GCCTCACCAT
    TCACAGTCAC CTCAAGTTGG AACACCTCAG CGAGAGCCTC AAAGAAATTT TTACCCAGCC
    GCACAGAACC TTCAAGCCAG TACTCAGCAG GCAACTTCTG GAGCATTATT TACACAGACA
    CCCTCAGGAC AATCTTCAGC AACATACAGT CAGTTTAATC AGACAAGTCT GAGCAGCACG
    GCACCACCCC CTCCACCCCC TCCACCCCCT TCTTCTTCGT CTTATTATCA AAACCAGCAG
    CCCTCTGCAA ACTTTCAGAA TTATAATCAG CTTAAAGGTA GTCTTTCCCA ACAAACTGTG
    TTTACATCAG GACCAAATCA AGCACTTCCT GGCACCACAA GCCAGCAAAC AGTTCCAGGA
    CACCACGTTA CTCCAGGGCA TTTTTTGCCT TCTCAGAACC CTACTGTTCA CCATCAGACT
    GCTGCTGCTG TGGTCCCACC CCCTCCTCCA CCCCCACCTG CTCCAGGACC ACACCTCGTA
    CAACAGCCAA GCTCCCATCA GCCACACTCA GTAGCACATG TGGTGGGGCC TGTTCACGCT
    GTCACCCCTG GATCGCATAT TCATTCTCAA ACTGCTGGGC ACCATTTGCC ACCACCGCCT
    CCCCCTCCTG GTCCTGCCCC TCATCACCAC CCCCCGCCCC ACCCTTCCAC AGGACTCCAA
    GGTCTACAAG CACAACACCA GCATGTGGTA AACTCAGCCC CTCCACCTCC CCCTCCTCCT
    CCGCCTTCCA GTGTTCTGGC TTCTGGGCAT CACACCACGT CAGCTCAAGC CTTACACCAT
    CCACCTCATC AAGGACCGCC ACTTTTTCCT TCAAGTGCTC ATCCAGCTGT ACCACCGTAT
    CCCTCACAAG CTACACATCA CACCACTCTG GGACTGGGAC CCCAACACCA GCCTTCTGGA
    ACAGGGCCAC ATTGTCCATT ACCAGTTGCA GGTCCTCATC TCCAGCCCCA AGGACCAAAC
    AGTATTCCAA CACCTACCGC TTCAGGGTTC TGTCCTCACC CTGGCTCTGT GGCCCTGCCA
    CATGGGGTCC AAGGACCTCA GCAGGCATCT CCAGTGCCTG GACAGATTCC AATTCACAGA
    GCACAGGTGC CACCGACATT TCAGAACAAT TACCATGGTT CGGGGTGGCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205423.1
    CDS450..5903
    Translation

    Target ORF information:

    RefSeq Version XM_005205366.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205366.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGCATAG TGATCCCATT GGGGGTTGAT ACAGCAGAAA CGTCATACTT GGAGATGGCT 
    GCAGGCTCAG AACCAGAATC CGTAGAAGCT AGCCCTGTGG TAGTTGAGAA ATCCAACAGT
    TATCCCCACC AGTTATATAC CAGCAGCTCG CATCATTCAC ACAGTTACAT TGGTTTGCCC
    TATGCGGACC ATAATTATGG TGCTCGTCCT CCTCCAACAC CTCCAGCTTC CCCTCCTCCA
    TCAGTTCTTA TTAGCAAAAA TGAAGTAAGC ATCTTTACCA CTCCTAATTT TGATGAAACT
    TCCAGTGCTA CTACAATCAG CACATCTGAG GATGGAAGTT ATGGTACTGA TGTAACCAGG
    TGCATATGTG GTTTTACACA TGATGATGGA TACATGATCT GTTGTGACAA ATGCAGTGTT
    TGGCAACATA TTGACTGCAT GGGGATTGAC AGGCAGCATA TTCCTGATAC ATATCTTTGT
    GAACGTTGTC AGCCTAGGAG TTTGGATAAA GAGAGGGCGG TGCTACTACA ACGCCGGAAA
    AGGGAAAATA TGTCAGATGG TGATACCAGT GCAACTGAGA GTGGTGATGA GGTTCCTGTG
    GAATTATATA CTGCATTTCA GCATACCCCA ACATCGATTA CTTTAACTGC TTCAAGGGTT
    TCTAAGGTTA ATGATAAAAG AAGGAAAAAA AGTGGGGAGA AAGAACAAAA TATTTCAAAA
    TGTAAAAAAG CCTTTCGTGA AGGATCTAGG AAATCATCAA GAGTTAAGGG CTCAGCTCCG
    GAGATTGATC CTTCATCTGA TGTTTCAAAT TTTGGATGGG AGACAAAAAT CAAAGCATGG
    ATGGATCAAT ATGAAGAGGC AAATAATAAC CAATATAGTG AGAGTGTTCA GAGGGAGGCA
    CAAAGAATAG CTCTGAGATT GGGCAATGGA AATGACAAAA AAGAGATTAA ATCAGATTTG
    AATACCAACA ATTTGATCTT CAAACCTCCT GTAGAGAGCC ATATACAAAA AAATAAGAAG
    ATTCTGAAAT CTGCAAAAGA TTTGCCTCCT GATGCACTTA TCATTGAATA CAGAGGGAAG
    TTTATGTTGA GAGAGCAGTT TGAAGCAAAT GGATATTTCT TTAAGAGACC ATACCCTTTT
    GTTTTATTCT ATTCTAAATT TCATGGCCTA GAAATGTGTG TTGATGCAAG GACTTTTGGG
    AATGAAGCTC GGTTCATCAG GCGTTCTTGT ACACCCAATG CAGAGGTGAG GCATGAAATT
    GAAGATGGAA CCATACATCT TTATATTTAT TCTATACAAA GTATTCCAAA GGGAACCGAA
    ATTACTATTG CCTTTGATTT TGACTATGGG AATTGTAAGT ACAAGGTGGA TTGTGCATGC
    CTCAAAGAAA ATTCAGAGTG CCCTGTTCTG AAACGTAGTT CTGAATCCAC GGAGAACATC
    AATAGCGGTT ATGAGACCAG AAGGAAGAAA GGCAAAAAAG AAAAGGATAT TTCAAAAGAA
    AAAGACACAC AAAATCAGAA TATTACTCTG GATTGTGAAG GAACAACGAA CAAAATGAAG
    AGCCCAGAAA CTAAACAGAG AAAGCTTTCT CCACTGAGAC TATCAGTATC AAATAATCAG
    GAACCAGATT TTATTGATGA TATAGAAGAA AAAACTCCCA TCAGCAATGA GGTAGAAATG
    GAGTCTGAGG AACAGATTGC AGAGAGGAAA AGGAAGATGA CAAGAGAAGA AAGGAAAATG
    GAAGCAATTC TGCAGGCTTT TGCCAGACTT GAAAAAAGAG AGAAGAGAAG AGAACAAGCT
    TTGGAAAGAA TCAGCACAGC CAAAACTGAA GTTAAAACTG AATGTAAAGA TGCACAGACT
    GTCAGTGATG CTGAAGTTAT TCAGGAACAA GCAAAAGAAG AAACTGCTAG CAAGCCAACC
    CCAGCCAAAG TAAATAGAAC TAAACAGAGA AAAAGTTTTT CTCGGAGCAG GACTCACATT
    GGACAGCAGC GTCGGAGACA CAGGACTGTC AGTATGTGTT CGGATATCCA ACCGTCTTCT
    CCTGACATCG AAGTTACCTC ACAACAAAAC GATGTGGAAA ATACTGTACT TGCAATAGAA
    CCAGAAACTG AAACTGCACT AACAGAAATA ATTACTGAAA ATGAAGTTCC AGTACTTAAT
    AAATGTCCAA CAAAGTACCC TAAAACAAAG AAGCACTTGG TCAGTGAATG GTTAAGTGAG
    AAGAATGAGA AGACAGGAAA ACCTTCAGAC AGCCTTTCAG AGAGGCCGCT GCGCATAACT
    ACAGATCCTG AAGTGCTAGC TACACAGCTC AATTCTTTGC CAGGTCTCAC TTACAGTCCA
    CATGTATACT CTACTCCTAA GCACTATATT AGATTCACTT CACCATTCCT TTCGGAAAAA
    AGGAGAAGAA AAGAACCTCC TGAAAACATA TCTGGCTCAT GCAAGAAGCG GTGGTTGAAA
    CAAGCTCTGG AAGAGGAAAA CTCAGCAATT TTACATAGAT TTAATTCGCC CTGTCAAGAA
    AGATCCAGAA GTCCTACAGT CAATGGTGAA AATAAAAGTC CACTACTATT AAATGATAGC
    TGTTCCCTTC CAGATTTAAC TACACCACTG AAAAAACGAA GACTTTATCA GTTGCTAGAT
    TCTGCTTACT CAGAAACCTC CACACCTACT CCTTCACCGT ATGCTACACC AACTCACACA
    GATATTACTC CTACAGACCC ATCATTTTCC ACTCCTCCAC GGATAAAGTC AGATGATGAA
    GCTTGTAGAA ATGGTTATAA GCCCATATAT TCACCAGTTA CCCCAGTAAC TCCTGGCACA
    CCAGGAAATA CCATGCACTT TGAGAATATT TCTTCCCCAG AAAGTTCTCC AGAAATTAAG
    AGACGCACTT ATAGTCAAGA GGGATATGAC AGATCGTCAA CCATGTTAAC GTTGGGGCCT
    TTTAGAAATT CTAACTTAAC TGAACTGGGT CTGCAAGAAA TAAAGACTAT TGGCTATGGC
    AGCCCTAGGA GTAGGACTGA AATCAGCAGG CAGTGCCCTG GAGAAAAGGA ACCGGTGTCA
    GATCTTCAAC TGGGACTCGA TGCAGTCGAG CAGACCGCCT TACATAAAAC CATGGAAGCA
    GCTGCACACG ACAGGACTGA GGCTGCCAGC CAGCTGGAGG CTGCTCACTC GGGGCGGGGC
    CCTCTGTATT CCTCCTGGGT AAAGAGTCCT GAGAGAACAG GCGTTAACTT CTCAGTAAAT
    TCCAACTTGA GGGACCTGAC ACCCTCACAT CAGTTGGAGG TTGGAGGCGG CTTCCGAATA
    AGTGAGTCAA AGTGCCTGAT GCAGGATGAT ACTAGAGGCA TGTTTATGGA AACACCCGTG
    TTTTGTACTT CAGAAGATGG GCTTGTATCT GGTTTCGGAC GGACTGTTAA TGACAATTTG
    ATCGACGGAA GTTGCACACC CCAGAATCCA CCACAAAAGA AAAAGGTTTC TCTATTAGAA
    TACCGGAAGA GACAACGTGA AGCTAGGAAG AGCGGCTCTA AGACGGAGCA CTTTGCGCTG
    GTTAGTGTAT CACCTCACAC AAGTGGCAAC TTGAGCAGCA GCACTGGTGA TGGGTGTGCC
    CACAGGAGTG AAAATGGGGA GCAGGTAGAA AGCGCTGCTA GCCTACCTTT ACCAACACCA
    GCTACAGTTT ATCATGCTAC TTCGGAAGAA ACCAGCAATA ACAGTACTGT TAAGGAAGTG
    TCTGCCAGTG AAAAGAATGA ACCAGAAGTT CAATGGACGG CCTCAACTTC AGTGGAGCAA
    GTGAGAGAGA GGAGTTATCA GAGAGCTCTG CTTCTCAGTG ATCACCGAAA AGATAAAGAC
    AGTGATCCTG ATCCTGAAAA TCTAGAACCC ACAACTACAA ATGAATGCCC ATCCCCAGAT
    ACTTCCCAAA GTACTTGTAA AAGTCCTTCA AAAATGAGCA AGCCTGGTTC ACCTGGACCT
    GTAATTCCTG TTCAGCCACA TGGGAAAATA CTTACAAAAC CAGATTCCCA TTGGGAGGGA
    ACAGTTATTG TATCTGAAGC TGAGAATGGT GTCCACCTGA AAACAGAACT CCATCAAAAA
    CAGCTGTCAA GTAACCCCCA AGCACTTTCA AAGAACCATC CTCCACAGCC ACTCGTTCGC
    TGTTCATCTG AGCAGCTTTC ACAAAAGCTG CCTTCTGCAC CCATGAAGTT GCACTGTCCT
    CCGTCACCTC ACGTAGAAAA TCCTCCAAAG TCATCGACGC CTCACACGCC TGTACAGCAT
    GGCTATCTCT CACCAAAGCC TCCTACACAG CAGTTAGGCT CTCCCTATAG GCCTCACCAT
    TCACAGTCAC CTCAAGTTGG AACACCTCAG CGAGAGCCTC AAAGAAATTT TTACCCAGCC
    GCACAGAACC TTCAAGCCAG TACTCAGCAG GCAACTTCTG GAGCATTATT TACACAGACA
    CCCTCAGGAC AATCTTCAGC AACATACAGT CAGTTTAATC AGACAAGTCT GAGCAGCACG
    GCACCACCCC CTCCACCCCC TCCACCCCCT TCTTCTTCGT CTTATTATCA AAACCAGCAG
    CCCTCTGCAA ACTTTCAGAA TTATAATCAG CTTAAAGGTA GTCTTTCCCA ACAAACTGTG
    TTTACATCAG GACCAAATCA AGCACTTCCT GGCACCACAA GCCAGCAAAC AGTTCCAGGA
    CACCACGTTA CTCCAGGGCA TTTTTTGCCT TCTCAGAACC CTACTGTTCA CCATCAGACT
    GCTGCTGCTG TGGTCCCACC CCCTCCTCCA CCCCCACCTG CTCCAGGACC ACACCTCGTA
    CAACAGCCAA GCTCCCATCA GCCACACTCA GTAGCACATG TGGTGGGGCC TGTTCACGCT
    GTCACCCCTG GATCGCATAT TCATTCTCAA ACTGCTGGGC ACCATTTGCC ACCACCGCCT
    CCCCCTCCTG GTCCTGCCCC TCATCACCAC CCCCCGCCCC ACCCTTCCAC AGGACTCCAA
    GGTCTACAAG CACAACACCA GCATGTGGTA AACTCAGCCC CTCCACCTCC CCCTCCTCCT
    CCGCCTTCCA GTGTTCTGGC TTCTGGGCAT CACACCACGT CAGCTCAAGC CTTACACCAT
    CCACCTCATC AAGGACCGCC ACTTTTTCCT TCAAGTGCTC ATCCAGCTGT ACCACCGTAT
    CCCTCACAAG CTACACATCA CACCACTCTG GGACTGGGAC CCCAACACCA GCCTTCTGGA
    ACAGGGCCAC ATTGTCCATT ACCAGTTGCA GGTCCTCATC TCCAGCCCCA AGGACCAAAC
    AGTATTCCAA CACCTACCGC TTCAGGGTTC TGTCCTCACC CTGGCTCTGT GGCCCTGCCA
    CATGGGGTCC AAGGACCTCA GCAGGCATCT CCAGTGCCTG GACAGATTCC AATTCACAGA
    GCACAGGTGC CACCGACATT TCAGAACAAT TACCATGGTT CGGGGTGGCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18521
    Clone ID Related Accession (Same CDS sequence) XM_005205367.3
    Accession Version XM_005205367.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase 2E isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205367.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGATTG ACAGGCAGCA TATTCCTGAT ACATATCTTT GTGAACGTTG TCAGCCTAGG 
    AGTTTGGATA AAGAGAGGGC GGTGCTACTA CAACGCCGGA AAAGGGAAAA TATGTCAGAT
    GGTGATACCA GTGCAACTGA GAGTGGTGAT GAGGTTCCTG TGGAATTATA TACTGCATTT
    CAGCATACCC CAACATCGAT TACTTTAACT GCTTCAAGGG TTTCTAAGGT TAATGATAAA
    AGAAGGAAAA AAAGTGGGGA GAAAGAACAA AATATTTCAA AATGTAAAAA AGCCTTTCGT
    GAAGGATCTA GGAAATCATC AAGAGTTAAG GGCTCAGCTC CGGAGATTGA TCCTTCATCT
    GATGTTTCAA ATTTTGGATG GGAGACAAAA ATCAAAGCAT GGATGGATCA ATATGAAGAG
    GCAAATAATA ACCAATATAG TGAGAGTGTT CAGAGGGAGG CACAAAGAAT AGCTCTGAGA
    TTGGGCAATG GAAATGACAA AAAAGAGATT AAATCAGATT TGAATACCAA CAATTTGATC
    TTCAAACCTC CTGTAGAGAG CCATATACAA AAAAATAAGA AGATTCTGAA ATCTGCAAAA
    GATTTGCCTC CTGATGCACT TATCATTGAA TACAGAGGGA AGTTTATGTT GAGAGAGCAG
    TTTGAAGCAA ATGGATATTT CTTTAAGAGA CCATACCCTT TTGTTTTATT CTATTCTAAA
    TTTCATGGCC TAGAAATGTG TGTTGATGCA AGGACTTTTG GGAATGAAGC TCGGTTCATC
    AGGCGTTCTT GTACACCCAA TGCAGAGGTG AGGCATGAAA TTGAAGATGG AACCATACAT
    CTTTATATTT ATTCTATACA AAGTATTCCA AAGGGAACCG AAATTACTAT TGCCTTTGAT
    TTTGACTATG GGAATTGTAA GTACAAGGTG GATTGTGCAT GCCTCAAAGA AAATTCAGAG
    TGCCCTGTTC TGAAACGTAG TTCTGAATCC ACGGAGAACA TCAATAGCGG TTATGAGACC
    AGAAGGAAGA AAGGCAAAAA AGAAAAGGAT ATTTCAAAAG AAAAAGACAC ACAAAATCAG
    AATATTACTC TGGATTGTGA AGGAACAACG AACAAAATGA AGAGCCCAGA AACTAAACAG
    AGAAAGCTTT CTCCACTGAG ACTATCAGTA TCAAATAATC AGGAACCAGA TTTTATTGAT
    GATATAGAAG AAAAAACTCC CATCAGCAAT GAGGTAGAAA TGGAGTCTGA GGAACAGATT
    GCAGAGAGGA AAAGGAAGAT GACAAGAGAA GAAAGGAAAA TGGAAGCAAT TCTGCAGGCT
    TTTGCCAGAC TTGAAAAAAG AGAGAAGAGA AGAGAACAAG CTTTGGAAAG AATCAGCACA
    GCCAAAACTG AAGTTAAAAC TGAATGTAAA GATGCACAGA CTGTCAGTGA TGCTGAAGTT
    ATTCAGGAAC AAGCAAAAGA AGAAACTGCT AGCAAGCCAA CCCCAGCCAA AGTAAATAGA
    ACTAAACAGA GAAAAAGTTT TTCTCGGAGC AGGACTCACA TTGGACAGCA GCGTCGGAGA
    CACAGGACTG TCAGTATGTG TTCGGATATC CAACCGTCTT CTCCTGACAT CGAAGTTACC
    TCACAACAAA ACGATGTGGA AAATACTGTA CTTGCAATAG AACCAGAAAC TGAAACTGCA
    CTAACAGAAA TAATTACTGA AAATGAAGTT CCAGTACTTA ATAAATGTCC AACAAAGTAC
    CCTAAAACAA AGAAGCACTT GGTCAGTGAA TGGTTAAGTG AGAAGAATGA GAAGACAGGA
    AAACCTTCAG ACAGCCTTTC AGAGAGGCCG CTGCGCATAA CTACAGATCC TGAAGTGCTA
    GCTACACAGC TCAATTCTTT GCCAGGTCTC ACTTACAGTC CACATGTATA CTCTACTCCT
    AAGCACTATA TTAGATTCAC TTCACCATTC CTTTCGGAAA AAAGGAGAAG AAAAGAACCT
    CCTGAAAACA TATCTGGCTC ATGCAAGAAG CGGTGGTTGA AACAAGCTCT GGAAGAGGAA
    AACTCAGCAA TTTTACATAG ATTTAATTCG CCCTGTCAAG AAAGATCCAG AAGTCCTACA
    GTCAATGGTG AAAATAAAAG TCCACTACTA TTAAATGATA GCTGTTCCCT TCCAGATTTA
    ACTACACCAC TGAAAAAACG AAGACTTTAT CAGTTGCTAG ATTCTGCTTA CTCAGAAACC
    TCCACACCTA CTCCTTCACC GTATGCTACA CCAACTCACA CAGATATTAC TCCTACAGAC
    CCATCATTTT CCACTCCTCC ACGGATAAAG TCAGATGATG AAGCTTGTAG AAATGGTTAT
    AAGCCCATAT ATTCACCAGT TACCCCAGTA ACTCCTGGCA CACCAGGAAA TACCATGCAC
    TTTGAGAATA TTTCTTCCCC AGAAAGTTCT CCAGAAATTA AGAGACGCAC TTATAGTCAA
    GAGGGATATG ACAGATCGTC AACCATGTTA ACGTTGGGGC CTTTTAGAAA TTCTAACTTA
    ACTGAACTGG GTCTGCAAGA AATAAAGACT ATTGGCTATG GCAGCCCTAG GAGTAGGACT
    GAAATCAGCA GGCAGTGCCC TGGAGAAAAG GAACCGGTGT CAGATCTTCA ACTGGGACTC
    GATGCAGTCG AGCAGACCGC CTTACATAAA ACCATGGAAG CAGCTGCACA CGACAGGACT
    GAGGCTGCCA GCCAGCTGGA GGCTGCTCAC TCGGGGCGGG GCCCTCTGTA TTCCTCCTGG
    GTAAAGAGTC CTGAGAGAAC AGGCGTTAAC TTCTCAGTAA ATTCCAACTT GAGGGACCTG
    ACACCCTCAC ATCAGTTGGA GGTTGGAGGC GGCTTCCGAA TAAGTGAGTC AAAGTGCCTG
    ATGCAGGATG ATACTAGAGG CATGTTTATG GAAACACCCG TGTTTTGTAC TTCAGAAGAT
    GGGCTTGTAT CTGGTTTCGG ACGGACTGTT AATGACAATT TGATCGACGG AAGTTGCACA
    CCCCAGAATC CACCACAAAA GAAAAAGGTT TCTCTATTAG AATACCGGAA GAGACAACGT
    GAAGCTAGGA AGAGCGGCTC TAAGACGGAG CACTTTGCGC TGGTTAGTGT ATCACCTCAC
    ACAAGTGGCA ACTTGAGCAG CAGCACTGGT GATGGGTGTG CCCACAGGAG TGAAAATGGG
    GAGCAGGTAG AAAGCGCTGC TAGCCTACCT TTACCAACAC CAGCTACAGT TTATCATGCT
    ACTTCGGAAG AAACCAGCAA TAACAGTACT GTTAAGGAAG TGTCTGCCAG TGAAAAGAAT
    GAACCAGAAG TTCAATGGAC GGCCTCAACT TCAGTGGAGC AAGTGAGAGA GAGGAGTTAT
    CAGAGAGCTC TGCTTCTCAG TGATCACCGA AAAGATAAAG ACAGTGGGGC AGAGTCACCA
    TGTGTCCCCT GTTCACCGAA TCATGTTCCG TCTTCTCCTT CATCTCATTC AAATCACATT
    CCCCAGTTGC AACCCAAGGG CCCAGTCCCT TCTTTCAGTG AACTTACGGA AGATCCTGAT
    CCTGAAAATC TAGAACCCAC AACTACAAAT GAATGCCCAT CCCCAGATAC TTCCCAAAGT
    ACTTGTAAAA GTCCTTCAAA AATGAGCAAG CCTGGTTCAC CTGGACCTGT AATTCCTGTT
    CAGCCACATG GGAAAATACT TACAAAACCA GATTCCCATT GGGAGGGAAC AGTTATTGTA
    TCTGAAGCTG AGAATGGTGT CCACCTGAAA ACAGAACTCC ATCAAAAACA GCTGTCAAGT
    AACCCCCAAG CACTTTCAAA GAACCATCCT CCACAGCCAC TCGTTCGCTG TTCATCTGAG
    CAGCTTTCAC AAAAGCTGCC TTCTGCACCC ATGAAGTTGC ACTGTCCTCC GTCACCTCAC
    GTAGAAAATC CTCCAAAGTC ATCGACGCCT CACACGCCTG TACAGCATGG CTATCTCTCA
    CCAAAGCCTC CTACACAGCA GTTAGGCTCT CCCTATAGGC CTCACCATTC ACAGTCACCT
    CAAGTTGGAA CACCTCAGCG AGAGCCTCAA AGAAATTTTT ACCCAGCCGC ACAGAACCTT
    CAAGCCAGTA CTCAGCAGGC AACTTCTGGA GCATTATTTA CACAGACACC CTCAGGACAA
    TCTTCAGCAA CATACAGTCA GTTTAATCAG ACAAGTCTGA GCAGCACGGC ACCACCCCCT
    CCACCCCCTC CACCCCCTTC TTCTTCGTCT TATTATCAAA ACCAGCAGCC CTCTGCAAAC
    TTTCAGAATT ATAATCAGCT TAAAGGTAGT CTTTCCCAAC AAACTGTGTT TACATCAGGA
    CCAAATCAAG CACTTCCTGG CACCACAAGC CAGCAAACAG TTCCAGGACA CCACGTTACT
    CCAGGGCATT TTTTGCCTTC TCAGAACCCT ACTGTTCACC ATCAGACTGC TGCTGCTGTG
    GTCCCACCCC CTCCTCCACC CCCACCTGCT CCAGGACCAC ACCTCGTACA ACAGCCAAGC
    TCCCATCAGC CACACTCAGT AGCACATGTG GTGGGGCCTG TTCACGCTGT CACCCCTGGA
    TCGCATATTC ATTCTCAAAC TGCTGGGCAC CATTTGCCAC CACCGCCTCC CCCTCCTGGT
    CCTGCCCCTC ATCACCACCC CCCGCCCCAC CCTTCCACAG GACTCCAAGG TCTACAAGCA
    CAACACCAGC ATGTGGTAAA CTCAGCCCCT CCACCTCCCC CTCCTCCTCC GCCTTCCAGT
    GTTCTGGCTT CTGGGCATCA CACCACGTCA GCTCAAGCCT TACACCATCC ACCTCATCAA
    GGACCGCCAC TTTTTCCTTC AAGTGCTCAT CCAGCTGTAC CACCGTATCC CTCACAAGCT
    ACACATCACA CCACTCTGGG ACTGGGACCC CAACACCAGC CTTCTGGAAC AGGGCCACAT
    TGTCCATTAC CAGTTGCAGG TCCTCATCTC CAGCCCCAAG GACCAAACAG TATTCCAACA
    CCTACCGCTT CAGGGTTCTG TCCTCACCCT GGCTCTGTGG CCCTGCCACA TGGGGTCCAA
    GGACCTCAGC AGGCATCTCC AGTGCCTGGA CAGATTCCAA TTCACAGAGC ACAGGTGCCA
    CCGACATTTC AGAACAATTA CCATGGTTCG GGGTGGCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205424.1
    CDS205..5346
    Translation

    Target ORF information:

    RefSeq Version XM_005205367.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific methyltransferase 2E (KMT2E), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205367.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGATTG ACAGGCAGCA TATTCCTGAT ACATATCTTT GTGAACGTTG TCAGCCTAGG 
    AGTTTGGATA AAGAGAGGGC GGTGCTACTA CAACGCCGGA AAAGGGAAAA TATGTCAGAT
    GGTGATACCA GTGCAACTGA GAGTGGTGAT GAGGTTCCTG TGGAATTATA TACTGCATTT
    CAGCATACCC CAACATCGAT TACTTTAACT GCTTCAAGGG TTTCTAAGGT TAATGATAAA
    AGAAGGAAAA AAAGTGGGGA GAAAGAACAA AATATTTCAA AATGTAAAAA AGCCTTTCGT
    GAAGGATCTA GGAAATCATC AAGAGTTAAG GGCTCAGCTC CGGAGATTGA TCCTTCATCT
    GATGTTTCAA ATTTTGGATG GGAGACAAAA ATCAAAGCAT GGATGGATCA ATATGAAGAG
    GCAAATAATA ACCAATATAG TGAGAGTGTT CAGAGGGAGG CACAAAGAAT AGCTCTGAGA
    TTGGGCAATG GAAATGACAA AAAAGAGATT AAATCAGATT TGAATACCAA CAATTTGATC
    TTCAAACCTC CTGTAGAGAG CCATATACAA AAAAATAAGA AGATTCTGAA ATCTGCAAAA
    GATTTGCCTC CTGATGCACT TATCATTGAA TACAGAGGGA AGTTTATGTT GAGAGAGCAG
    TTTGAAGCAA ATGGATATTT CTTTAAGAGA CCATACCCTT TTGTTTTATT CTATTCTAAA
    TTTCATGGCC TAGAAATGTG TGTTGATGCA AGGACTTTTG GGAATGAAGC TCGGTTCATC
    AGGCGTTCTT GTACACCCAA TGCAGAGGTG AGGCATGAAA TTGAAGATGG AACCATACAT
    CTTTATATTT ATTCTATACA AAGTATTCCA AAGGGAACCG AAATTACTAT TGCCTTTGAT
    TTTGACTATG GGAATTGTAA GTACAAGGTG GATTGTGCAT GCCTCAAAGA AAATTCAGAG
    TGCCCTGTTC TGAAACGTAG TTCTGAATCC ACGGAGAACA TCAATAGCGG TTATGAGACC
    AGAAGGAAGA AAGGCAAAAA AGAAAAGGAT ATTTCAAAAG AAAAAGACAC ACAAAATCAG
    AATATTACTC TGGATTGTGA AGGAACAACG AACAAAATGA AGAGCCCAGA AACTAAACAG
    AGAAAGCTTT CTCCACTGAG ACTATCAGTA TCAAATAATC AGGAACCAGA TTTTATTGAT
    GATATAGAAG AAAAAACTCC CATCAGCAAT GAGGTAGAAA TGGAGTCTGA GGAACAGATT
    GCAGAGAGGA AAAGGAAGAT GACAAGAGAA GAAAGGAAAA TGGAAGCAAT TCTGCAGGCT
    TTTGCCAGAC TTGAAAAAAG AGAGAAGAGA AGAGAACAAG CTTTGGAAAG AATCAGCACA
    GCCAAAACTG AAGTTAAAAC TGAATGTAAA GATGCACAGA CTGTCAGTGA TGCTGAAGTT
    ATTCAGGAAC AAGCAAAAGA AGAAACTGCT AGCAAGCCAA CCCCAGCCAA AGTAAATAGA
    ACTAAACAGA GAAAAAGTTT TTCTCGGAGC AGGACTCACA TTGGACAGCA GCGTCGGAGA
    CACAGGACTG TCAGTATGTG TTCGGATATC CAACCGTCTT CTCCTGACAT CGAAGTTACC
    TCACAACAAA ACGATGTGGA AAATACTGTA CTTGCAATAG AACCAGAAAC TGAAACTGCA
    CTAACAGAAA TAATTACTGA AAATGAAGTT CCAGTACTTA ATAAATGTCC AACAAAGTAC
    CCTAAAACAA AGAAGCACTT GGTCAGTGAA TGGTTAAGTG AGAAGAATGA GAAGACAGGA
    AAACCTTCAG ACAGCCTTTC AGAGAGGCCG CTGCGCATAA CTACAGATCC TGAAGTGCTA
    GCTACACAGC TCAATTCTTT GCCAGGTCTC ACTTACAGTC CACATGTATA CTCTACTCCT
    AAGCACTATA TTAGATTCAC TTCACCATTC CTTTCGGAAA AAAGGAGAAG AAAAGAACCT
    CCTGAAAACA TATCTGGCTC ATGCAAGAAG CGGTGGTTGA AACAAGCTCT GGAAGAGGAA
    AACTCAGCAA TTTTACATAG ATTTAATTCG CCCTGTCAAG AAAGATCCAG AAGTCCTACA
    GTCAATGGTG AAAATAAAAG TCCACTACTA TTAAATGATA GCTGTTCCCT TCCAGATTTA
    ACTACACCAC TGAAAAAACG AAGACTTTAT CAGTTGCTAG ATTCTGCTTA CTCAGAAACC
    TCCACACCTA CTCCTTCACC GTATGCTACA CCAACTCACA CAGATATTAC TCCTACAGAC
    CCATCATTTT CCACTCCTCC ACGGATAAAG TCAGATGATG AAGCTTGTAG AAATGGTTAT
    AAGCCCATAT ATTCACCAGT TACCCCAGTA ACTCCTGGCA CACCAGGAAA TACCATGCAC
    TTTGAGAATA TTTCTTCCCC AGAAAGTTCT CCAGAAATTA AGAGACGCAC TTATAGTCAA
    GAGGGATATG ACAGATCGTC AACCATGTTA ACGTTGGGGC CTTTTAGAAA TTCTAACTTA
    ACTGAACTGG GTCTGCAAGA AATAAAGACT ATTGGCTATG GCAGCCCTAG GAGTAGGACT
    GAAATCAGCA GGCAGTGCCC TGGAGAAAAG GAACCGGTGT CAGATCTTCA ACTGGGACTC
    GATGCAGTCG AGCAGACCGC CTTACATAAA ACCATGGAAG CAGCTGCACA CGACAGGACT
    GAGGCTGCCA GCCAGCTGGA GGCTGCTCAC TCGGGGCGGG GCCCTCTGTA TTCCTCCTGG
    GTAAAGAGTC CTGAGAGAAC AGGCGTTAAC TTCTCAGTAA ATTCCAACTT GAGGGACCTG
    ACACCCTCAC ATCAGTTGGA GGTTGGAGGC GGCTTCCGAA TAAGTGAGTC AAAGTGCCTG
    ATGCAGGATG ATACTAGAGG CATGTTTATG GAAACACCCG TGTTTTGTAC TTCAGAAGAT
    GGGCTTGTAT CTGGTTTCGG ACGGACTGTT AATGACAATT TGATCGACGG AAGTTGCACA
    CCCCAGAATC CACCACAAAA GAAAAAGGTT TCTCTATTAG AATACCGGAA GAGACAACGT
    GAAGCTAGGA AGAGCGGCTC TAAGACGGAG CACTTTGCGC TGGTTAGTGT ATCACCTCAC
    ACAAGTGGCA ACTTGAGCAG CAGCACTGGT GATGGGTGTG CCCACAGGAG TGAAAATGGG
    GAGCAGGTAG AAAGCGCTGC TAGCCTACCT TTACCAACAC CAGCTACAGT TTATCATGCT
    ACTTCGGAAG AAACCAGCAA TAACAGTACT GTTAAGGAAG TGTCTGCCAG TGAAAAGAAT
    GAACCAGAAG TTCAATGGAC GGCCTCAACT TCAGTGGAGC AAGTGAGAGA GAGGAGTTAT
    CAGAGAGCTC TGCTTCTCAG TGATCACCGA AAAGATAAAG ACAGTGGGGC AGAGTCACCA
    TGTGTCCCCT GTTCACCGAA TCATGTTCCG TCTTCTCCTT CATCTCATTC AAATCACATT
    CCCCAGTTGC AACCCAAGGG CCCAGTCCCT TCTTTCAGTG AACTTACGGA AGATCCTGAT
    CCTGAAAATC TAGAACCCAC AACTACAAAT GAATGCCCAT CCCCAGATAC TTCCCAAAGT
    ACTTGTAAAA GTCCTTCAAA AATGAGCAAG CCTGGTTCAC CTGGACCTGT AATTCCTGTT
    CAGCCACATG GGAAAATACT TACAAAACCA GATTCCCATT GGGAGGGAAC AGTTATTGTA
    TCTGAAGCTG AGAATGGTGT CCACCTGAAA ACAGAACTCC ATCAAAAACA GCTGTCAAGT
    AACCCCCAAG CACTTTCAAA GAACCATCCT CCACAGCCAC TCGTTCGCTG TTCATCTGAG
    CAGCTTTCAC AAAAGCTGCC TTCTGCACCC ATGAAGTTGC ACTGTCCTCC GTCACCTCAC
    GTAGAAAATC CTCCAAAGTC ATCGACGCCT CACACGCCTG TACAGCATGG CTATCTCTCA
    CCAAAGCCTC CTACACAGCA GTTAGGCTCT CCCTATAGGC CTCACCATTC ACAGTCACCT
    CAAGTTGGAA CACCTCAGCG AGAGCCTCAA AGAAATTTTT ACCCAGCCGC ACAGAACCTT
    CAAGCCAGTA CTCAGCAGGC AACTTCTGGA GCATTATTTA CACAGACACC CTCAGGACAA
    TCTTCAGCAA CATACAGTCA GTTTAATCAG ACAAGTCTGA GCAGCACGGC ACCACCCCCT
    CCACCCCCTC CACCCCCTTC TTCTTCGTCT TATTATCAAA ACCAGCAGCC CTCTGCAAAC
    TTTCAGAATT ATAATCAGCT TAAAGGTAGT CTTTCCCAAC AAACTGTGTT TACATCAGGA
    CCAAATCAAG CACTTCCTGG CACCACAAGC CAGCAAACAG TTCCAGGACA CCACGTTACT
    CCAGGGCATT TTTTGCCTTC TCAGAACCCT ACTGTTCACC ATCAGACTGC TGCTGCTGTG
    GTCCCACCCC CTCCTCCACC CCCACCTGCT CCAGGACCAC ACCTCGTACA ACAGCCAAGC
    TCCCATCAGC CACACTCAGT AGCACATGTG GTGGGGCCTG TTCACGCTGT CACCCCTGGA
    TCGCATATTC ATTCTCAAAC TGCTGGGCAC CATTTGCCAC CACCGCCTCC CCCTCCTGGT
    CCTGCCCCTC ATCACCACCC CCCGCCCCAC CCTTCCACAG GACTCCAAGG TCTACAAGCA
    CAACACCAGC ATGTGGTAAA CTCAGCCCCT CCACCTCCCC CTCCTCCTCC GCCTTCCAGT
    GTTCTGGCTT CTGGGCATCA CACCACGTCA GCTCAAGCCT TACACCATCC ACCTCATCAA
    GGACCGCCAC TTTTTCCTTC AAGTGCTCAT CCAGCTGTAC CACCGTATCC CTCACAAGCT
    ACACATCACA CCACTCTGGG ACTGGGACCC CAACACCAGC CTTCTGGAAC AGGGCCACAT
    TGTCCATTAC CAGTTGCAGG TCCTCATCTC CAGCCCCAAG GACCAAACAG TATTCCAACA
    CCTACCGCTT CAGGGTTCTG TCCTCACCCT GGCTCTGTGG CCCTGCCACA TGGGGTCCAA
    GGACCTCAGC AGGCATCTCC AGTGCCTGGA CAGATTCCAA TTCACAGAGC ACAGGTGCCA
    CCGACATTTC AGAACAATTA CCATGGTTCG GGGTGGCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.