MAST2 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005204881.2	XP_005204938.1	microtubule-associated serine/threonine-protein kinase 2 isoform X2
XM_005204883.3	XP_005204940.1	microtubule-associated serine/threonine-protein kinase 2 isoform X5
XM_005204886.3	XP_005204943.1	microtubule-associated serine/threonine-protein kinase 2 isoform X8
XM_015462789.1	XP_015318275.1	microtubule-associated serine/threonine-protein kinase 2 isoform X6
XM_005204880.3	XP_005204937.2	microtubule-associated serine/threonine-protein kinase 2 isoform X1
XM_003585907.4	XP_003585955.2	microtubule-associated serine/threonine-protein kinase 2 isoform X3
XM_005204886.4	XP_005204943.1	microtubule-associated serine/threonine-protein kinase 2 isoform X9
XM_003585907.3	XP_003585955.1	microtubule-associated serine/threonine-protein kinase 2 isoform X3
XM_015462784.1	XP_015318270.1	microtubule-associated serine/threonine-protein kinase 2 isoform X1
XM_015462786.1	XP_015318272.1	microtubule-associated serine/threonine-protein kinase 2 isoform X3
XM_015462788.1	XP_015318274.1	microtubule-associated serine/threonine-protein kinase 2 isoform X5
XM_003582019.4	XP_003582067.2	microtubule-associated serine/threonine-protein kinase 2 isoform X7
XM_005204884.3	XP_005204941.1	microtubule-associated serine/threonine-protein kinase 2 isoform X6
XM_015462785.1	XP_015318271.1	microtubule-associated serine/threonine-protein kinase 2 isoform X2
XM_005204881.3	XP_005204938.2	microtubule-associated serine/threonine-protein kinase 2 isoform X2
XM_024990152.1	XP_024845920.1	microtubule-associated serine/threonine-protein kinase 2 isoform X5
XM_005204883.4	XP_005204940.1	microtubule-associated serine/threonine-protein kinase 2 isoform X6
XM_015462787.1	XP_015318273.1	microtubule-associated serine/threonine-protein kinase 2 isoform X4
XM_005204880.2	XP_005204937.1	microtubule-associated serine/threonine-protein kinase 2 isoform X1
XM_005204882.3	XP_005204939.2	microtubule-associated serine/threonine-protein kinase 2 isoform X4
XM_005204882.2	XP_005204939.1	microtubule-associated serine/threonine-protein kinase 2 isoform X4
XM_010803741.2	XP_010802043.1	microtubule-associated serine/threonine-protein kinase 2 isoform X7
XM_015462790.1	XP_015318276.1	microtubule-associated serine/threonine-protein kinase 2 isoform X8
XM_005204884.4	XP_005204941.1	microtubule-associated serine/threonine-protein kinase 2 isoform X7
XM_010803741.3	XP_010802043.1	microtubule-associated serine/threonine-protein kinase 2 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa233318	XM_005204880.3 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233319	XM_005204881.3 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233320	XM_003585907.4 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233321	XM_005204882.3 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233322	XM_005204883.4 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233323	XM_005204884.4 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa53214	XM_024990152.1 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa18162	XM_005204883.3 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa18162	XM_015462788.1 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa18163	XM_005204884.3 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa18163	XM_015462789.1 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa18165	XM_005204886.4 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa18165	XM_015462790.1 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa53217	XM_010803741.3 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa53217	XM_003582019.4 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa18158	XM_015462784.1 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18158	XM_005204880.2 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18159	XM_015462785.1 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18159	XM_005204881.2 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18160	XM_015462786.1 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18160	XM_003585907.3 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18161	XM_005204882.2 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18161	XM_015462787.1 Latest version!	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OBa18165	XM_005204886.3	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa53217	XM_010803741.2	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

MAST2 ( XM_005204880.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204937.2 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa233318
Clone ID Related Accession (Same CDS sequence)	XM_005204880.3
Accession Version	XM_005204880.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA 
CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT 
ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG 
GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG 
GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA 
GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG 
GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC 
GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC 
CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT 
CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG 
GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC 
CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC 
CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG 
GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT 
ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA 
CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG 
TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG 
AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT 
GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC 
ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC 
CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA 
CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG 
GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA 
AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC 
GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG 
GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT 
GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG 
GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC 
CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT 
GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA 
GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG 
GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT 
CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG 
ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG 
GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG 
CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG 
CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT 
GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG 
GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA 
CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC 
CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC 
CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA 
GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC 
TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG 
GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC 
ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT 
GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC 
AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC 
GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT 
TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC 
AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGCAGA 
AAAAGGAGCT CCCTGTTCCG GAAGATCACC AAGCAGGCAT CCCTGCTCCA CACCAGCCGC 
AGCCTTTCTT CCCTTAACCG CTCCCTGTCG TCCGGCGAGA GTGGGCCAGG CTCGCCCACA 
CACAGCCACA GCCTTTCTCC CCGCTCTCCC ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC 
GTGCACTCAG TGGGGGGGAA TTCATCACAG AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT 
TCTCCTGCCG GCTCTGGGCA CACACGGCCG AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC 
CAACGCCAGT ACCGCTCTCC ACGGCGCAAG TCAGCAGGCA GCATCCCGCT GTCACCACTG 
GCCCACACCC CTTCACCACC ACCAGCGGCT TCGCCTCAGC GTTCCCCATC ACCTCTGTCT 
GGCCACGGGG CCCAGGCCTT CCCTACCAAG CTCCACTTGT CGCCTCCCCT AGGCCGGCAA 
CTCTCACGGC CCAAGAGCGC GGAGCCACCC CGCTCACCAC TACTTAAGAG GGTGCAGTCA 
GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA 
CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC 
TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA 
GGGGTGTTGC AGGCTGCTCC CTCACGGGCC TTAGGGACCC TCCGCCAGGA CAGGGCTGAA 
CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC ATCCGGGAGG TTGACTCTTC AGAAGATGAT 
ACTGATGAGG GGCCTGAGAA CAGCCAAGGT GTTCAGGAGC CAAGCTTGGT ACCCCACCGA 
GAAGGGGGCC AGGATCCACC CCTCAGAGGA GCAGGAGAGG GTGAGGAAGA GGATGCCTTC 
TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA GTGGTCACAG GTCTACTGAC AGGGGTCACA 
CTGGGATCTC CCAGAATGGA AGGCCCTGGT GTCCCCCAGA AGAAGCTTGG AATCCTACAG 
GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC 
ACAGACCCCA TCCCCGCTGA AGGCTGCTGG AAGGCCCAGC ACCTCCACAC CCAGGCACTG 
ACAGCACTTT GTCCGAGCTC ATCAGGACGC ATCCCTGCCA GTTGCTCCAC TGCCTCCACC 
CCTGGAGAGC TGGGCCCATG GTCCTGGAAA TTCCTCTTCA AGGGCCCTGA CAGGGCATAC 
CCAACCGGAA AGGCAGCAAT GGAAGATGGG CCAGCCAGCT CCCAAGATTT GGAAACTACA 
ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC AGACAGGAGG GGAAGTCATG GCCACCCAGT 
GCCCCTGGGC TGGCCCATCC ACCTTGTGAG GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT 
GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA GCGCTGGGCA TCACCAAAGT GCCTGATGCC 
TCAGGTGACA GGAGGCAGGA CGTTCCATGC AAGAGCTGCC CCTTCACCCC AGAGCCTGGG 
CCCAGCCTGC TCCAGAAGGG CCAAGACCCA GGGGGCCCTC AGGAGCATCA GGACTTGGCA 
TTGTTACCTG ATGAGCTTTT AAAGCAAACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204937.2
CDS	307..5739
Translation	MKRSRCRDRPQPPPPPLPQAAGREDGAQRAAELPQPQPLPPRRRAPPGRQRLEERTGPVGPEVREQDVVTGRSPLLFRKLSNPDIFSSTGKAKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSGSAHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_005204880.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204880.3

ORF Insert Sequence:

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA 
CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT 
ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG 
GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG 
GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA 
GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG 
GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC 
GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC 
CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT 
CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG 
GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC 
CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC 
CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG 
GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT 
ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA 
CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG 
TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG 
AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT 
GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC 
ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC 
CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA 
CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG 
GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA 
AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC 
GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG 
GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT 
GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG 
GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC 
CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT 
GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA 
GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG 
GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT 
CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG 
ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG 
GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG 
CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG 
CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT 
GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG 
GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA 
CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC 
CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC 
CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA 
GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC 
TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG 
GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC 
ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT 
GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC 
AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC 
GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT 
TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC 
AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGCAGA 
AAAAGGAGCT CCCTGTTCCG GAAGATCACC AAGCAGGCAT CCCTGCTCCA CACCAGCCGC 
AGCCTTTCTT CCCTTAACCG CTCCCTGTCG TCCGGCGAGA GTGGGCCAGG CTCGCCCACA 
CACAGCCACA GCCTTTCTCC CCGCTCTCCC ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC 
GTGCACTCAG TGGGGGGGAA TTCATCACAG AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT 
TCTCCTGCCG GCTCTGGGCA CACACGGCCG AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC 
CAACGCCAGT ACCGCTCTCC ACGGCGCAAG TCAGCAGGCA GCATCCCGCT GTCACCACTG 
GCCCACACCC CTTCACCACC ACCAGCGGCT TCGCCTCAGC GTTCCCCATC ACCTCTGTCT 
GGCCACGGGG CCCAGGCCTT CCCTACCAAG CTCCACTTGT CGCCTCCCCT AGGCCGGCAA 
CTCTCACGGC CCAAGAGCGC GGAGCCACCC CGCTCACCAC TACTTAAGAG GGTGCAGTCA 
GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA 
CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC 
TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA 
GGGGTGTTGC AGGCTGCTCC CTCACGGGCC TTAGGGACCC TCCGCCAGGA CAGGGCTGAA 
CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC ATCCGGGAGG TTGACTCTTC AGAAGATGAT 
ACTGATGAGG GGCCTGAGAA CAGCCAAGGT GTTCAGGAGC CAAGCTTGGT ACCCCACCGA 
GAAGGGGGCC AGGATCCACC CCTCAGAGGA GCAGGAGAGG GTGAGGAAGA GGATGCCTTC 
TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA GTGGTCACAG GTCTACTGAC AGGGGTCACA 
CTGGGATCTC CCAGAATGGA AGGCCCTGGT GTCCCCCAGA AGAAGCTTGG AATCCTACAG 
GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC 
ACAGACCCCA TCCCCGCTGA AGGCTGCTGG AAGGCCCAGC ACCTCCACAC CCAGGCACTG 
ACAGCACTTT GTCCGAGCTC ATCAGGACGC ATCCCTGCCA GTTGCTCCAC TGCCTCCACC 
CCTGGAGAGC TGGGCCCATG GTCCTGGAAA TTCCTCTTCA AGGGCCCTGA CAGGGCATAC 
CCAACCGGAA AGGCAGCAAT GGAAGATGGG CCAGCCAGCT CCCAAGATTT GGAAACTACA 
ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC AGACAGGAGG GGAAGTCATG GCCACCCAGT 
GCCCCTGGGC TGGCCCATCC ACCTTGTGAG GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT 
GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA GCGCTGGGCA TCACCAAAGT GCCTGATGCC 
TCAGGTGACA GGAGGCAGGA CGTTCCATGC AAGAGCTGCC CCTTCACCCC AGAGCCTGGG 
CCCAGCCTGC TCCAGAAGGG CCAAGACCCA GGGGGCCCTC AGGAGCATCA GGACTTGGCA 
TTGTTACCTG ATGAGCTTTT AAAGCAAACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_005204881.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204938.2 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.

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CloneID	OBa233319
Clone ID Related Accession (Same CDS sequence)	XM_005204881.3
Accession Version	XM_005204881.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204881.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA 
CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT 
ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG 
GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG 
GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA 
GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG 
GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC 
GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC 
CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT 
CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG 
GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC 
CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC 
CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG 
GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT 
ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA 
CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG 
TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG 
AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT 
GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC 
ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC 
CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA 
CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG 
GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA 
AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC 
GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG 
GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT 
GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG 
GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC 
CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT 
GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA 
GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG 
GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT 
CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG 
ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG 
GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG 
CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG 
CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT 
GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG 
GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA 
CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC 
CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC 
CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA 
GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC 
TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG 
GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC 
ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT 
GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC 
AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC 
GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT 
TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC 
AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGAAAA 
AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC 
CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC 
AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG 
CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT 
CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA 
CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC 
CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC 
CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC 
TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT 
GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG 
AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT 
CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG 
GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG 
CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT 
GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA 
GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG 
CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG 
GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT 
TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA 
GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA 
GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT 
GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA 
ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT 
CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC 
CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG 
TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA 
GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC 
AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG 
TTACCTGATG AGCTTTTAAA GCAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204938.2
CDS	307..5736
Translation	MKRSRCRDRPQPPPPPLPQAAGREDGAQRAAELPQPQPLPPRRRAPPGRQRLEERTGPVGPEVREQDVVTGRSPLLFRKLSNPDIFSSTGKAKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSGSAHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQERKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_005204881.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204881.3

ORF Insert Sequence:

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA 
CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT 
ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG 
GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG 
GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA 
GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG 
GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC 
GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC 
CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT 
CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG 
GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC 
CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC 
CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG 
GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT 
ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA 
CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG 
TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG 
AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT 
GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC 
ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC 
CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA 
CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG 
GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA 
AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC 
GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG 
GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT 
GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG 
GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC 
CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT 
GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA 
GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG 
GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT 
CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG 
ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG 
GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG 
CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG 
CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT 
GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG 
GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA 
CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC 
CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC 
CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA 
GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC 
TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG 
GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC 
ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT 
GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC 
AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC 
GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT 
TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC 
AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGAAAA 
AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC 
CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC 
AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG 
CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT 
CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA 
CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC 
CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC 
CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC 
TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT 
GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG 
AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT 
CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG 
GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG 
CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT 
GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA 
GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG 
CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG 
GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT 
TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA 
GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA 
GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT 
GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA 
ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT 
CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC 
CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG 
TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA 
GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC 
AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG 
TTACCTGATG AGCTTTTAAA GCAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_003585907.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003585955.2 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.

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CloneID	OBa233320
Clone ID Related Accession (Same CDS sequence)	XM_003585907.4
Accession Version	XM_003585907.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003585907.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA 
TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC 
TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG 
ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG 
CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA 
ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG 
CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA 
ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC 
CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT 
CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG 
AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC 
CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG 
ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA 
GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA 
GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA 
ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC 
ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC 
AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA 
GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC 
CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC 
CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT 
TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT 
GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA 
CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC 
ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC 
TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG 
GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA 
GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT 
CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT 
GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG 
GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC 
AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC 
AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG 
CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG 
TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC 
CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG 
CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG 
GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC 
CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC 
ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG 
GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC 
ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC 
ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC 
TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG 
TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG 
CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG 
GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG 
GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC 
ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG 
AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG 
AAGATCACCA AGCAGGCATC CCTGCTCCAC ACCAGCCGCA GCCTTTCTTC CCTTAACCGC 
TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC TCGCCCACAC ACAGCCACAG CCTTTCTCCC 
CGCTCTCCCA CGCAGGGCTA CCGGGTGACC CCTGAAGCCG TGCACTCAGT GGGGGGGAAT 
TCATCACAGA GCAGCTCCCC CAGCTCCAGC GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC 
ACACGGCCGA GCTCCCTCCA CGGTCTGGCG CCCAAGCTCC AACGCCAGTA CCGCTCTCCA 
CGGCGCAAGT CAGCAGGCAG CATCCCGCTG TCACCACTGG CCCACACCCC TTCACCACCA 
CCAGCGGCTT CGCCTCAGCG TTCCCCATCA CCTCTGTCTG GCCACGGGGC CCAGGCCTTC 
CCTACCAAGC TCCACTTGTC GCCTCCCCTA GGCCGGCAAC TCTCACGGCC CAAGAGCGCG 
GAGCCACCCC GCTCACCACT ACTTAAGAGG GTGCAGTCAG CTGAAAAACT GGCAGCTGCA 
CTGGCTGCTT CTGAGAAGAA GCTCGCCACT TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC 
CCCGAGCTAA AGAAGGAATT GCCGCCCAGG GAAGTCAGCT CTCTGGAGGT AGTTGGGACC 
AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC 
TCACGGGCCT TAGGGACCCT CCGCCAGGAC AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG 
CAGGAAGCCA TCCGGGAGGT TGACTCTTCA GAAGATGATA CTGATGAGGG GCCTGAGAAC 
AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA CCCCACCGAG AAGGGGGCCA GGATCCACCC 
CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG GATGCCTTCT TGCCCAGGGA TCCCAGGGGA 
CAGGGCCCAG TGGTCACAGG TCTACTGACA GGGGTCACAC TGGGATCTCC CAGAATGGAA 
GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA ATCCTACAGG CTTTTGAAGA GGGTTCCAGC 
TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG GCTGGCCCCA CAGACCCCAT CCCCGCTGAA 
GGCTGCTGGA AGGCCCAGCA CCTCCACACC CAGGCACTGA CAGCACTTTG TCCGAGCTCA 
TCAGGACGCA TCCCTGCCAG TTGCTCCACT GCCTCCACCC CTGGAGAGCT GGGCCCATGG 
TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC AGGGCATACC CAACCGGAAA GGCAGCAATG 
GAAGATGGGC CAGCCAGCTC CCAAGATTTG GAAACTACAA CTCCAGGCCA CCCTGCAAAC 
CTGTCCCCCA GACAGGAGGG GAAGTCATGG CCACCCAGTG CCCCTGGGCT GGCCCATCCA 
CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA TGGGAGTCTG AGTGTGCACA GAGAGAGAAA 
GAGGAGCCAG CGCTGGGCAT CACCAAAGTG CCTGATGCCT CAGGTGACAG GAGGCAGGAC 
GTTCCATGCA AGAGCTGCCC CTTCACCCCA GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC 
CAAGACCCAG GGGGCCCTCA GGAGCATCAG GACTTGGCAT TGTTACCTGA TGAGCTTTTA 
AAGCAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003585955.2
CDS	307..5718
Translation	MKRSRCRDRPQPPPPPLPQAAGREDGAQRAAELPQPQPLPPRRRAPPGRQRLEERTGPVGPEVREQDVVTGRSPLLFRKLSNPDIFSSTGKAKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSGSAHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_003585907.4
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003585907.4

ORF Insert Sequence:

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA 
TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC 
TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG 
ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG 
CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA 
ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG 
CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA 
ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC 
CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT 
CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG 
AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC 
CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG 
ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA 
GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA 
GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA 
ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC 
ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC 
AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA 
GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC 
CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC 
CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT 
TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT 
GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA 
CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC 
ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC 
TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG 
GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA 
GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT 
CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT 
GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG 
GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC 
AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC 
AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG 
CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG 
TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC 
CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG 
CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG 
GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC 
CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC 
ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG 
GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC 
ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC 
ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC 
TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG 
TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG 
CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG 
GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG 
GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC 
ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG 
AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG 
AAGATCACCA AGCAGGCATC CCTGCTCCAC ACCAGCCGCA GCCTTTCTTC CCTTAACCGC 
TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC TCGCCCACAC ACAGCCACAG CCTTTCTCCC 
CGCTCTCCCA CGCAGGGCTA CCGGGTGACC CCTGAAGCCG TGCACTCAGT GGGGGGGAAT 
TCATCACAGA GCAGCTCCCC CAGCTCCAGC GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC 
ACACGGCCGA GCTCCCTCCA CGGTCTGGCG CCCAAGCTCC AACGCCAGTA CCGCTCTCCA 
CGGCGCAAGT CAGCAGGCAG CATCCCGCTG TCACCACTGG CCCACACCCC TTCACCACCA 
CCAGCGGCTT CGCCTCAGCG TTCCCCATCA CCTCTGTCTG GCCACGGGGC CCAGGCCTTC 
CCTACCAAGC TCCACTTGTC GCCTCCCCTA GGCCGGCAAC TCTCACGGCC CAAGAGCGCG 
GAGCCACCCC GCTCACCACT ACTTAAGAGG GTGCAGTCAG CTGAAAAACT GGCAGCTGCA 
CTGGCTGCTT CTGAGAAGAA GCTCGCCACT TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC 
CCCGAGCTAA AGAAGGAATT GCCGCCCAGG GAAGTCAGCT CTCTGGAGGT AGTTGGGACC 
AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC 
TCACGGGCCT TAGGGACCCT CCGCCAGGAC AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG 
CAGGAAGCCA TCCGGGAGGT TGACTCTTCA GAAGATGATA CTGATGAGGG GCCTGAGAAC 
AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA CCCCACCGAG AAGGGGGCCA GGATCCACCC 
CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG GATGCCTTCT TGCCCAGGGA TCCCAGGGGA 
CAGGGCCCAG TGGTCACAGG TCTACTGACA GGGGTCACAC TGGGATCTCC CAGAATGGAA 
GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA ATCCTACAGG CTTTTGAAGA GGGTTCCAGC 
TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG GCTGGCCCCA CAGACCCCAT CCCCGCTGAA 
GGCTGCTGGA AGGCCCAGCA CCTCCACACC CAGGCACTGA CAGCACTTTG TCCGAGCTCA 
TCAGGACGCA TCCCTGCCAG TTGCTCCACT GCCTCCACCC CTGGAGAGCT GGGCCCATGG 
TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC AGGGCATACC CAACCGGAAA GGCAGCAATG 
GAAGATGGGC CAGCCAGCTC CCAAGATTTG GAAACTACAA CTCCAGGCCA CCCTGCAAAC 
CTGTCCCCCA GACAGGAGGG GAAGTCATGG CCACCCAGTG CCCCTGGGCT GGCCCATCCA 
CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA TGGGAGTCTG AGTGTGCACA GAGAGAGAAA 
GAGGAGCCAG CGCTGGGCAT CACCAAAGTG CCTGATGCCT CAGGTGACAG GAGGCAGGAC 
GTTCCATGCA AGAGCTGCCC CTTCACCCCA GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC 
CAAGACCCAG GGGGCCCTCA GGAGCATCAG GACTTGGCAT TGTTACCTGA TGAGCTTTTA 
AAGCAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_005204882.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204939.2 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.

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CloneID	OBa233321
Clone ID Related Accession (Same CDS sequence)	XM_005204882.3
Accession Version	XM_005204882.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204882.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA 
TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC 
TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG 
ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG 
CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA 
ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG 
CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA 
ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC 
CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT 
CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG 
AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC 
CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG 
ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA 
GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA 
GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA 
ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC 
ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC 
AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA 
GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC 
CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC 
CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT 
TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT 
GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA 
CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC 
ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC 
TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG 
GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA 
GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT 
CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT 
GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG 
GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC 
AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC 
AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG 
CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG 
TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC 
CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG 
CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG 
GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC 
CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC 
ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG 
GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC 
ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC 
ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC 
TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG 
TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG 
CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG 
GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG 
GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC 
ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG 
AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGAAAAA GGAGCTCCCT GTTCCGGAAG 
ATCACCAAGC AGGCATCCCT GCTCCACACC AGCCGCAGCC TTTCTTCCCT TAACCGCTCC 
CTGTCGTCCG GCGAGAGTGG GCCAGGCTCG CCCACACACA GCCACAGCCT TTCTCCCCGC 
TCTCCCACGC AGGGCTACCG GGTGACCCCT GAAGCCGTGC ACTCAGTGGG GGGGAATTCA 
TCACAGAGCA GCTCCCCCAG CTCCAGCGTG CCCAGTTCTC CTGCCGGCTC TGGGCACACA 
CGGCCGAGCT CCCTCCACGG TCTGGCGCCC AAGCTCCAAC GCCAGTACCG CTCTCCACGG 
CGCAAGTCAG CAGGCAGCAT CCCGCTGTCA CCACTGGCCC ACACCCCTTC ACCACCACCA 
GCGGCTTCGC CTCAGCGTTC CCCATCACCT CTGTCTGGCC ACGGGGCCCA GGCCTTCCCT 
ACCAAGCTCC ACTTGTCGCC TCCCCTAGGC CGGCAACTCT CACGGCCCAA GAGCGCGGAG 
CCACCCCGCT CACCACTACT TAAGAGGGTG CAGTCAGCTG AAAAACTGGC AGCTGCACTG 
GCTGCTTCTG AGAAGAAGCT CGCCACTTCT CGAAAACAGA GCCTTGACCT GCCCCACCCC 
GAGCTAAAGA AGGAATTGCC GCCCAGGGAA GTCAGCTCTC TGGAGGTAGT TGGGACCAGG 
AGTGTGCTGT CTGGAAAGGG GGCGCTGCCA GGGAAAGGGG TGTTGCAGGC TGCTCCCTCA 
CGGGCCTTAG GGACCCTCCG CCAGGACAGG GCTGAACGGC GAGAGTCGCT GCAGAAGCAG 
GAAGCCATCC GGGAGGTTGA CTCTTCAGAA GATGATACTG ATGAGGGGCC TGAGAACAGC 
CAAGGTGTTC AGGAGCCAAG CTTGGTACCC CACCGAGAAG GGGGCCAGGA TCCACCCCTC 
AGAGGAGCAG GAGAGGGTGA GGAAGAGGAT GCCTTCTTGC CCAGGGATCC CAGGGGACAG 
GGCCCAGTGG TCACAGGTCT ACTGACAGGG GTCACACTGG GATCTCCCAG AATGGAAGGC 
CCTGGTGTCC CCCAGAAGAA GCTTGGAATC CTACAGGCTT TTGAAGAGGG TTCCAGCTCC 
TCGTCCTCAG CCCCCAACCT GGGGAAGGCT GGCCCCACAG ACCCCATCCC CGCTGAAGGC 
TGCTGGAAGG CCCAGCACCT CCACACCCAG GCACTGACAG CACTTTGTCC GAGCTCATCA 
GGACGCATCC CTGCCAGTTG CTCCACTGCC TCCACCCCTG GAGAGCTGGG CCCATGGTCC 
TGGAAATTCC TCTTCAAGGG CCCTGACAGG GCATACCCAA CCGGAAAGGC AGCAATGGAA 
GATGGGCCAG CCAGCTCCCA AGATTTGGAA ACTACAACTC CAGGCCACCC TGCAAACCTG 
TCCCCCAGAC AGGAGGGGAA GTCATGGCCA CCCAGTGCCC CTGGGCTGGC CCATCCACCT 
TGTGAGGTTC CCAGCCAGAG CTGGCTATGG GAGTCTGAGT GTGCACAGAG AGAGAAAGAG 
GAGCCAGCGC TGGGCATCAC CAAAGTGCCT GATGCCTCAG GTGACAGGAG GCAGGACGTT 
CCATGCAAGA GCTGCCCCTT CACCCCAGAG CCTGGGCCCA GCCTGCTCCA GAAGGGCCAA 
GACCCAGGGG GCCCTCAGGA GCATCAGGAC TTGGCATTGT TACCTGATGA GCTTTTAAAG 
CAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204939.2
CDS	307..5715
Translation	MKRSRCRDRPQPPPPPLPQAAGREDGAQRAAELPQPQPLPPRRRAPPGRQRLEERTGPVGPEVREQDVVTGRSPLLFRKLSNPDIFSSTGKAKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSGSAHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQERKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_005204882.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204882.3

ORF Insert Sequence:

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA 
TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC 
TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG 
ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG 
CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA 
ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG 
CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA 
ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC 
CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT 
CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG 
AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC 
CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG 
ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA 
GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA 
GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA 
ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC 
ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC 
AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA 
GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC 
CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC 
CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT 
TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT 
GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA 
CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC 
ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC 
TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG 
GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA 
GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT 
CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT 
GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG 
GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC 
AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC 
AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG 
CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG 
TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC 
CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG 
CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG 
GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC 
CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC 
ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG 
GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC 
ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC 
ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC 
TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG 
TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG 
CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG 
GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG 
GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC 
ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG 
AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGAAAAA GGAGCTCCCT GTTCCGGAAG 
ATCACCAAGC AGGCATCCCT GCTCCACACC AGCCGCAGCC TTTCTTCCCT TAACCGCTCC 
CTGTCGTCCG GCGAGAGTGG GCCAGGCTCG CCCACACACA GCCACAGCCT TTCTCCCCGC 
TCTCCCACGC AGGGCTACCG GGTGACCCCT GAAGCCGTGC ACTCAGTGGG GGGGAATTCA 
TCACAGAGCA GCTCCCCCAG CTCCAGCGTG CCCAGTTCTC CTGCCGGCTC TGGGCACACA 
CGGCCGAGCT CCCTCCACGG TCTGGCGCCC AAGCTCCAAC GCCAGTACCG CTCTCCACGG 
CGCAAGTCAG CAGGCAGCAT CCCGCTGTCA CCACTGGCCC ACACCCCTTC ACCACCACCA 
GCGGCTTCGC CTCAGCGTTC CCCATCACCT CTGTCTGGCC ACGGGGCCCA GGCCTTCCCT 
ACCAAGCTCC ACTTGTCGCC TCCCCTAGGC CGGCAACTCT CACGGCCCAA GAGCGCGGAG 
CCACCCCGCT CACCACTACT TAAGAGGGTG CAGTCAGCTG AAAAACTGGC AGCTGCACTG 
GCTGCTTCTG AGAAGAAGCT CGCCACTTCT CGAAAACAGA GCCTTGACCT GCCCCACCCC 
GAGCTAAAGA AGGAATTGCC GCCCAGGGAA GTCAGCTCTC TGGAGGTAGT TGGGACCAGG 
AGTGTGCTGT CTGGAAAGGG GGCGCTGCCA GGGAAAGGGG TGTTGCAGGC TGCTCCCTCA 
CGGGCCTTAG GGACCCTCCG CCAGGACAGG GCTGAACGGC GAGAGTCGCT GCAGAAGCAG 
GAAGCCATCC GGGAGGTTGA CTCTTCAGAA GATGATACTG ATGAGGGGCC TGAGAACAGC 
CAAGGTGTTC AGGAGCCAAG CTTGGTACCC CACCGAGAAG GGGGCCAGGA TCCACCCCTC 
AGAGGAGCAG GAGAGGGTGA GGAAGAGGAT GCCTTCTTGC CCAGGGATCC CAGGGGACAG 
GGCCCAGTGG TCACAGGTCT ACTGACAGGG GTCACACTGG GATCTCCCAG AATGGAAGGC 
CCTGGTGTCC CCCAGAAGAA GCTTGGAATC CTACAGGCTT TTGAAGAGGG TTCCAGCTCC 
TCGTCCTCAG CCCCCAACCT GGGGAAGGCT GGCCCCACAG ACCCCATCCC CGCTGAAGGC 
TGCTGGAAGG CCCAGCACCT CCACACCCAG GCACTGACAG CACTTTGTCC GAGCTCATCA 
GGACGCATCC CTGCCAGTTG CTCCACTGCC TCCACCCCTG GAGAGCTGGG CCCATGGTCC 
TGGAAATTCC TCTTCAAGGG CCCTGACAGG GCATACCCAA CCGGAAAGGC AGCAATGGAA 
GATGGGCCAG CCAGCTCCCA AGATTTGGAA ACTACAACTC CAGGCCACCC TGCAAACCTG 
TCCCCCAGAC AGGAGGGGAA GTCATGGCCA CCCAGTGCCC CTGGGCTGGC CCATCCACCT 
TGTGAGGTTC CCAGCCAGAG CTGGCTATGG GAGTCTGAGT GTGCACAGAG AGAGAAAGAG 
GAGCCAGCGC TGGGCATCAC CAAAGTGCCT GATGCCTCAG GTGACAGGAG GCAGGACGTT 
CCATGCAAGA GCTGCCCCTT CACCCCAGAG CCTGGGCCCA GCCTGCTCCA GAAGGGCCAA 
GACCCAGGGG GCCCTCAGGA GCATCAGGAC TTGGCATTGT TACCTGATGA GCTTTTAAAG 
CAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_005204883.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204940.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA.

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CloneID	OBa233322
Clone ID Related Accession (Same CDS sequence)	XM_005204883.4
Accession Version	XM_005204883.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204883.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG 
TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT 
TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGTCCCT GTTCCAGCCG CTCACTGCAG 
TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA 
CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC 
CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGCCATGGC 
CACAGAGCAG ACAGGACTGA TGGACGGCGC TGGTCTTTGG CCTCATTGCC TTCTTCAGGC 
TATGGAACCA ACACCCCTAG TTCTACCGTG TCATCGTCGT GCTCCTCTCA GGAGAAGCTG 
CACCAGTTGC CTTTCCAGCC CACGGCCGAT GAGCTGCACT TCTTGACGAA GCATTTCAGC 
ACAGACAGTG TGCCTGATGA GGAGGGGCGG CAGTCCCCCG CCCTGCGGCC CCGCTCCCGC 
AGCCTCAGTC CTGGACGGTC ACCAGTTTCC TTTGACAGTG AAATAATAAT GATGAATCAT 
GTATACAAGG AAAGATTCCC CAAGGCCACT GCACAAATGG AAGAGCGTCT GGCCGAGTTC 
ATCTCCTCCA ATACTCCAGA CAACGTGTTG CCCTTGGCTG ATGGAACCTT GAGCTTTATT 
CATCATCAGG TGATAGAGAT GGCCCGAGAC TGCCTGGACA AATCCCGGAG TGGCCTCATT 
ACCTCACACT ACTTCTACGA ACTTCAAGAG AATTTAGAGA AGCTTCTGCA AGATGCTCAT 
GAGCGCTCAG AGAGTTCAGA AGTGGCTTTT GTGATGCAGC TGGTGAAAAA GCTGATGATT 
GTCATTGCCC GCCCAGCTCG TCTCCTGGAA TGCCTGGAGT TTGACCCTGA AGAGTTCTAC 
CACCTGTTAG AAGCAGCTGA GGGCCACGCC AAAGAGGGAC AAGGGATTAA ATGTGACATT 
CCCCGCTACA TCGTTAGCCA ACTGGGCCTC ACACGGGATC CCCTAGAGGA AATGGCCCAG 
TTGAGCAGCT ATGACAGTCC TGATACTCCG GAGACAGATG ATTCAGTCGA GGGCCGTGGG 
GCATCTCTGA CATCGAAAAA GACACCCTCT GAGGAGGACT TCGAAACCAT TAAGCTCATC 
AGCAATGGCG CCTATGGGGC CGTGTTCCTG GTGCGGCACA AGTCCACCCG GCAGCGCTTT 
GCCATGAAGA AGATCAACAA GCAGAACCTG ATCCTGCGGA ACCAGATCCA GCAGGCCTTC 
GTGGAGCGGG ACATCCTGAC TTTTGCTGAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC 
TTTGAAACCA AGCGCCACCT GTGCATGGTG ATGGAGTATG TGGAAGGAGG AGACTGTGCC 
ACGCTGCTGA AGAACATCGG GGCCCTGCCT GTGGACATGG TGCGCCTGTA CTTTGCAGAA 
ACTGTGCTAG CCCTGGAGTA CTTACACAAT TACGGCATCG TGCACCGTGA CCTCAAGCCT 
GACAACCTGC TGATTACATC CATGGGTCAC ATCAAGCTCA CTGATTTTGG CCTTTCCAAA 
ATTGGCCTTA TGAGTCTGAC CACAAACTTG TACGAGGGCC ACATTGAAAA GGATGCACGG 
GAGTTCCTGG ACAAGCAGGT ATGCGGGACC CCGGAGTACA TTGCACCTGA GGTGATCCTG 
CGGCAGGGCT ACGGGAAGCC AGTGGACTGG TGGGCTATGG GCATCATCCT GTACGAGTTC 
CTGGTGGGCT GCGTCCCTTT CTTTGGAGAC ACTCCGGAGG AGCTCTTTGG GCAAGTGATC 
AGTGATGAGA TTGTGTGGCC TGAGGGGGAC GATGCACTGC CGCCGGACGC CCAAGATCTC 
ACCTCAAAAC TGCTCCACCA GAACCCATTG GAGAGACTGG GCACAGGCAG TGCCTATGAG 
GTGAAGCAGC ACCCATTCTT CACTGGTCTG GACTGGACGG GACTTCTTCG CCAGAAGGCT 
GAATTTATTC CTCAGCTGGA GTCAGAGGAT GATACCAGCT ACTTTGACAC CCGCTCAGAG 
CGATACCACC ACGTGGACTC GGAGGATGAG GAGGAAGTGA GTGAGGATGG CTGCCTTGAG 
ATTCGCCAGT TCTCGTCCTG TTCTCCAAGG TTCAGCAAGG TGTACAGCAG CATGGAGCGG 
CTCTCGCTGC TCGAGGAGCG CCGGACGCCA CCCCCCACCA AGCGCAGCCT GAGTGAGGAA 
AAGGAGGACC GCACGGATGG CCTGGCGGGG CTGAGGGGCC GAGAGCGAAG CTGGGTGATC 
GGCTCCCCTG AGATATTACG GAAGCGGCTG TCGGTGTCCG AGTCGTCCCA CACGGAAAGC 
GATTCCAGCC CTCCACTCAC CGTGCGGCGC CACTGCTCGG GCCTCCTGGA CGTGCCTCGC 
TTCCCTGAGA GCACTGAGGA GGCTGTTGGC GCTCCCAGGA GGCAGCAGCA GGAGGGTGCA 
TGGCTTCTGA CCCCGTCTGG AGAGGGGGCG TCTGGGCCTG CCCTGGAACG GCCGGCCGAG 
CGGCGGCTGA AGCTGAATGA GGAACCTGCT GGCCAGAGCA GCGCCCCCAG CCCAGCCATG 
GAGACCCGAG GAGGCCGCGG AACCCCACAG CTGGCAGAAG GAGCCACAGC CAGGGCCATC 
AGCGACCTGG CGGTGCGCAG GGCCCGCCAC CGGCTGCTCT CTGGGGACTC CATAGAAAAG 
CGCACCGCTC GCCCCATCAA CAAAGTGATC AAGTCCGCCT CAGCCACAGC CCTGTCCCTT 
CTCATTCCTG CTGAGCACCA CTCCTGCTCT CCACTGGCCA GCCCCATGTC CCCACATTCC 
CAGTCATCCA ACCCATCATC CAGGGACTCT TCTCCAAGCA GGGACTTCCT GCCAGCCCTC 
AGCAGCTCAC GGCCCCCCAT TGTCATCCAC CGAGCCGGCA AGAAGTATGG CTTCACCCTA 
CGAGCCATCC GTGTCTACAT GGGTGACTCA GATGTCTACA CCGTGCACCA CATGGTGTGG 
CACGTGGAGG ACGGAGGTCC AGCCAGTGAG GCAGGGCTGC GTCAGGGGGA CCTCATCACG 
CACGTCAACG GGGAGCCTGT GCACGGGCTG GTGCACACGG AGGTGGTGGA GCTGATCCTG 
AAGAGTGGAA ACAAGGTGTC TATTTCAACA ACTCCCCTGG AGAACACCTC CATTAAGGTG 
GGGCCCGCTC GGAAGGGCAG CTACAAGGCC AAGATGGCCC GAAGGAGCAA GCGGAGCCGG 
GGCAAGGATG GACAAGAAAG CAGAAAAAGG AGCTCCCTGT TCCGGAAGAT CACCAAGCAG 
GCATCCCTGC TCCACACCAG CCGCAGCCTT TCTTCCCTTA ACCGCTCCCT GTCGTCCGGC 
GAGAGTGGGC CAGGCTCGCC CACACACAGC CACAGCCTTT CTCCCCGCTC TCCCACGCAG 
GGCTACCGGG TGACCCCTGA AGCCGTGCAC TCAGTGGGGG GGAATTCATC ACAGAGCAGC 
TCCCCCAGCT CCAGCGTGCC CAGTTCTCCT GCCGGCTCTG GGCACACACG GCCGAGCTCC 
CTCCACGGTC TGGCGCCCAA GCTCCAACGC CAGTACCGCT CTCCACGGCG CAAGTCAGCA 
GGCAGCATCC CGCTGTCACC ACTGGCCCAC ACCCCTTCAC CACCACCAGC GGCTTCGCCT 
CAGCGTTCCC CATCACCTCT GTCTGGCCAC GGGGCCCAGG CCTTCCCTAC CAAGCTCCAC 
TTGTCGCCTC CCCTAGGCCG GCAACTCTCA CGGCCCAAGA GCGCGGAGCC ACCCCGCTCA 
CCACTACTTA AGAGGGTGCA GTCAGCTGAA AAACTGGCAG CTGCACTGGC TGCTTCTGAG 
AAGAAGCTCG CCACTTCTCG AAAACAGAGC CTTGACCTGC CCCACCCCGA GCTAAAGAAG 
GAATTGCCGC CCAGGGAAGT CAGCTCTCTG GAGGTAGTTG GGACCAGGAG TGTGCTGTCT 
GGAAAGGGGG CGCTGCCAGG GAAAGGGGTG TTGCAGGCTG CTCCCTCACG GGCCTTAGGG 
ACCCTCCGCC AGGACAGGGC TGAACGGCGA GAGTCGCTGC AGAAGCAGGA AGCCATCCGG 
GAGGTTGACT CTTCAGAAGA TGATACTGAT GAGGGGCCTG AGAACAGCCA AGGTGTTCAG 
GAGCCAAGCT TGGTACCCCA CCGAGAAGGG GGCCAGGATC CACCCCTCAG AGGAGCAGGA 
GAGGGTGAGG AAGAGGATGC CTTCTTGCCC AGGGATCCCA GGGGACAGGG CCCAGTGGTC 
ACAGGTCTAC TGACAGGGGT CACACTGGGA TCTCCCAGAA TGGAAGGCCC TGGTGTCCCC 
CAGAAGAAGC TTGGAATCCT ACAGGCTTTT GAAGAGGGTT CCAGCTCCTC GTCCTCAGCC 
CCCAACCTGG GGAAGGCTGG CCCCACAGAC CCCATCCCCG CTGAAGGCTG CTGGAAGGCC 
CAGCACCTCC ACACCCAGGC ACTGACAGCA CTTTGTCCGA GCTCATCAGG ACGCATCCCT 
GCCAGTTGCT CCACTGCCTC CACCCCTGGA GAGCTGGGCC CATGGTCCTG GAAATTCCTC 
TTCAAGGGCC CTGACAGGGC ATACCCAACC GGAAAGGCAG CAATGGAAGA TGGGCCAGCC 
AGCTCCCAAG ATTTGGAAAC TACAACTCCA GGCCACCCTG CAAACCTGTC CCCCAGACAG 
GAGGGGAAGT CATGGCCACC CAGTGCCCCT GGGCTGGCCC ATCCACCTTG TGAGGTTCCC 
AGCCAGAGCT GGCTATGGGA GTCTGAGTGT GCACAGAGAG AGAAAGAGGA GCCAGCGCTG 
GGCATCACCA AAGTGCCTGA TGCCTCAGGT GACAGGAGGC AGGACGTTCC ATGCAAGAGC 
TGCCCCTTCA CCCCAGAGCC TGGGCCCAGC CTGCTCCAGA AGGGCCAAGA CCCAGGGGGC 
CCTCAGGAGC ATCAGGACTT GGCATTGTTA CCTGATGAGC TTTTAAAGCA AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204940.1
CDS	244..5400
Translation	MMKRRRERLGAPCLRIQISALCRGSEVNRHVLSPTSAPAWSLSKVPFSAECTLATSPLAFFLNTHAHSSPGSPCSSRSLQWSCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_005204883.4
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204883.4

ORF Insert Sequence:

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG 
TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT 
TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGTCCCT GTTCCAGCCG CTCACTGCAG 
TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA 
CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC 
CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGCCATGGC 
CACAGAGCAG ACAGGACTGA TGGACGGCGC TGGTCTTTGG CCTCATTGCC TTCTTCAGGC 
TATGGAACCA ACACCCCTAG TTCTACCGTG TCATCGTCGT GCTCCTCTCA GGAGAAGCTG 
CACCAGTTGC CTTTCCAGCC CACGGCCGAT GAGCTGCACT TCTTGACGAA GCATTTCAGC 
ACAGACAGTG TGCCTGATGA GGAGGGGCGG CAGTCCCCCG CCCTGCGGCC CCGCTCCCGC 
AGCCTCAGTC CTGGACGGTC ACCAGTTTCC TTTGACAGTG AAATAATAAT GATGAATCAT 
GTATACAAGG AAAGATTCCC CAAGGCCACT GCACAAATGG AAGAGCGTCT GGCCGAGTTC 
ATCTCCTCCA ATACTCCAGA CAACGTGTTG CCCTTGGCTG ATGGAACCTT GAGCTTTATT 
CATCATCAGG TGATAGAGAT GGCCCGAGAC TGCCTGGACA AATCCCGGAG TGGCCTCATT 
ACCTCACACT ACTTCTACGA ACTTCAAGAG AATTTAGAGA AGCTTCTGCA AGATGCTCAT 
GAGCGCTCAG AGAGTTCAGA AGTGGCTTTT GTGATGCAGC TGGTGAAAAA GCTGATGATT 
GTCATTGCCC GCCCAGCTCG TCTCCTGGAA TGCCTGGAGT TTGACCCTGA AGAGTTCTAC 
CACCTGTTAG AAGCAGCTGA GGGCCACGCC AAAGAGGGAC AAGGGATTAA ATGTGACATT 
CCCCGCTACA TCGTTAGCCA ACTGGGCCTC ACACGGGATC CCCTAGAGGA AATGGCCCAG 
TTGAGCAGCT ATGACAGTCC TGATACTCCG GAGACAGATG ATTCAGTCGA GGGCCGTGGG 
GCATCTCTGA CATCGAAAAA GACACCCTCT GAGGAGGACT TCGAAACCAT TAAGCTCATC 
AGCAATGGCG CCTATGGGGC CGTGTTCCTG GTGCGGCACA AGTCCACCCG GCAGCGCTTT 
GCCATGAAGA AGATCAACAA GCAGAACCTG ATCCTGCGGA ACCAGATCCA GCAGGCCTTC 
GTGGAGCGGG ACATCCTGAC TTTTGCTGAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC 
TTTGAAACCA AGCGCCACCT GTGCATGGTG ATGGAGTATG TGGAAGGAGG AGACTGTGCC 
ACGCTGCTGA AGAACATCGG GGCCCTGCCT GTGGACATGG TGCGCCTGTA CTTTGCAGAA 
ACTGTGCTAG CCCTGGAGTA CTTACACAAT TACGGCATCG TGCACCGTGA CCTCAAGCCT 
GACAACCTGC TGATTACATC CATGGGTCAC ATCAAGCTCA CTGATTTTGG CCTTTCCAAA 
ATTGGCCTTA TGAGTCTGAC CACAAACTTG TACGAGGGCC ACATTGAAAA GGATGCACGG 
GAGTTCCTGG ACAAGCAGGT ATGCGGGACC CCGGAGTACA TTGCACCTGA GGTGATCCTG 
CGGCAGGGCT ACGGGAAGCC AGTGGACTGG TGGGCTATGG GCATCATCCT GTACGAGTTC 
CTGGTGGGCT GCGTCCCTTT CTTTGGAGAC ACTCCGGAGG AGCTCTTTGG GCAAGTGATC 
AGTGATGAGA TTGTGTGGCC TGAGGGGGAC GATGCACTGC CGCCGGACGC CCAAGATCTC 
ACCTCAAAAC TGCTCCACCA GAACCCATTG GAGAGACTGG GCACAGGCAG TGCCTATGAG 
GTGAAGCAGC ACCCATTCTT CACTGGTCTG GACTGGACGG GACTTCTTCG CCAGAAGGCT 
GAATTTATTC CTCAGCTGGA GTCAGAGGAT GATACCAGCT ACTTTGACAC CCGCTCAGAG 
CGATACCACC ACGTGGACTC GGAGGATGAG GAGGAAGTGA GTGAGGATGG CTGCCTTGAG 
ATTCGCCAGT TCTCGTCCTG TTCTCCAAGG TTCAGCAAGG TGTACAGCAG CATGGAGCGG 
CTCTCGCTGC TCGAGGAGCG CCGGACGCCA CCCCCCACCA AGCGCAGCCT GAGTGAGGAA 
AAGGAGGACC GCACGGATGG CCTGGCGGGG CTGAGGGGCC GAGAGCGAAG CTGGGTGATC 
GGCTCCCCTG AGATATTACG GAAGCGGCTG TCGGTGTCCG AGTCGTCCCA CACGGAAAGC 
GATTCCAGCC CTCCACTCAC CGTGCGGCGC CACTGCTCGG GCCTCCTGGA CGTGCCTCGC 
TTCCCTGAGA GCACTGAGGA GGCTGTTGGC GCTCCCAGGA GGCAGCAGCA GGAGGGTGCA 
TGGCTTCTGA CCCCGTCTGG AGAGGGGGCG TCTGGGCCTG CCCTGGAACG GCCGGCCGAG 
CGGCGGCTGA AGCTGAATGA GGAACCTGCT GGCCAGAGCA GCGCCCCCAG CCCAGCCATG 
GAGACCCGAG GAGGCCGCGG AACCCCACAG CTGGCAGAAG GAGCCACAGC CAGGGCCATC 
AGCGACCTGG CGGTGCGCAG GGCCCGCCAC CGGCTGCTCT CTGGGGACTC CATAGAAAAG 
CGCACCGCTC GCCCCATCAA CAAAGTGATC AAGTCCGCCT CAGCCACAGC CCTGTCCCTT 
CTCATTCCTG CTGAGCACCA CTCCTGCTCT CCACTGGCCA GCCCCATGTC CCCACATTCC 
CAGTCATCCA ACCCATCATC CAGGGACTCT TCTCCAAGCA GGGACTTCCT GCCAGCCCTC 
AGCAGCTCAC GGCCCCCCAT TGTCATCCAC CGAGCCGGCA AGAAGTATGG CTTCACCCTA 
CGAGCCATCC GTGTCTACAT GGGTGACTCA GATGTCTACA CCGTGCACCA CATGGTGTGG 
CACGTGGAGG ACGGAGGTCC AGCCAGTGAG GCAGGGCTGC GTCAGGGGGA CCTCATCACG 
CACGTCAACG GGGAGCCTGT GCACGGGCTG GTGCACACGG AGGTGGTGGA GCTGATCCTG 
AAGAGTGGAA ACAAGGTGTC TATTTCAACA ACTCCCCTGG AGAACACCTC CATTAAGGTG 
GGGCCCGCTC GGAAGGGCAG CTACAAGGCC AAGATGGCCC GAAGGAGCAA GCGGAGCCGG 
GGCAAGGATG GACAAGAAAG CAGAAAAAGG AGCTCCCTGT TCCGGAAGAT CACCAAGCAG 
GCATCCCTGC TCCACACCAG CCGCAGCCTT TCTTCCCTTA ACCGCTCCCT GTCGTCCGGC 
GAGAGTGGGC CAGGCTCGCC CACACACAGC CACAGCCTTT CTCCCCGCTC TCCCACGCAG 
GGCTACCGGG TGACCCCTGA AGCCGTGCAC TCAGTGGGGG GGAATTCATC ACAGAGCAGC 
TCCCCCAGCT CCAGCGTGCC CAGTTCTCCT GCCGGCTCTG GGCACACACG GCCGAGCTCC 
CTCCACGGTC TGGCGCCCAA GCTCCAACGC CAGTACCGCT CTCCACGGCG CAAGTCAGCA 
GGCAGCATCC CGCTGTCACC ACTGGCCCAC ACCCCTTCAC CACCACCAGC GGCTTCGCCT 
CAGCGTTCCC CATCACCTCT GTCTGGCCAC GGGGCCCAGG CCTTCCCTAC CAAGCTCCAC 
TTGTCGCCTC CCCTAGGCCG GCAACTCTCA CGGCCCAAGA GCGCGGAGCC ACCCCGCTCA 
CCACTACTTA AGAGGGTGCA GTCAGCTGAA AAACTGGCAG CTGCACTGGC TGCTTCTGAG 
AAGAAGCTCG CCACTTCTCG AAAACAGAGC CTTGACCTGC CCCACCCCGA GCTAAAGAAG 
GAATTGCCGC CCAGGGAAGT CAGCTCTCTG GAGGTAGTTG GGACCAGGAG TGTGCTGTCT 
GGAAAGGGGG CGCTGCCAGG GAAAGGGGTG TTGCAGGCTG CTCCCTCACG GGCCTTAGGG 
ACCCTCCGCC AGGACAGGGC TGAACGGCGA GAGTCGCTGC AGAAGCAGGA AGCCATCCGG 
GAGGTTGACT CTTCAGAAGA TGATACTGAT GAGGGGCCTG AGAACAGCCA AGGTGTTCAG 
GAGCCAAGCT TGGTACCCCA CCGAGAAGGG GGCCAGGATC CACCCCTCAG AGGAGCAGGA 
GAGGGTGAGG AAGAGGATGC CTTCTTGCCC AGGGATCCCA GGGGACAGGG CCCAGTGGTC 
ACAGGTCTAC TGACAGGGGT CACACTGGGA TCTCCCAGAA TGGAAGGCCC TGGTGTCCCC 
CAGAAGAAGC TTGGAATCCT ACAGGCTTTT GAAGAGGGTT CCAGCTCCTC GTCCTCAGCC 
CCCAACCTGG GGAAGGCTGG CCCCACAGAC CCCATCCCCG CTGAAGGCTG CTGGAAGGCC 
CAGCACCTCC ACACCCAGGC ACTGACAGCA CTTTGTCCGA GCTCATCAGG ACGCATCCCT 
GCCAGTTGCT CCACTGCCTC CACCCCTGGA GAGCTGGGCC CATGGTCCTG GAAATTCCTC 
TTCAAGGGCC CTGACAGGGC ATACCCAACC GGAAAGGCAG CAATGGAAGA TGGGCCAGCC 
AGCTCCCAAG ATTTGGAAAC TACAACTCCA GGCCACCCTG CAAACCTGTC CCCCAGACAG 
GAGGGGAAGT CATGGCCACC CAGTGCCCCT GGGCTGGCCC ATCCACCTTG TGAGGTTCCC 
AGCCAGAGCT GGCTATGGGA GTCTGAGTGT GCACAGAGAG AGAAAGAGGA GCCAGCGCTG 
GGCATCACCA AAGTGCCTGA TGCCTCAGGT GACAGGAGGC AGGACGTTCC ATGCAAGAGC 
TGCCCCTTCA CCCCAGAGCC TGGGCCCAGC CTGCTCCAGA AGGGCCAAGA CCCAGGGGGC 
CCTCAGGAGC ATCAGGACTT GGCATTGTTA CCTGATGAGC TTTTAAAGCA AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_005204884.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204941.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA.

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CloneID	OBa233323
Clone ID Related Accession (Same CDS sequence)	XM_005204884.4
Accession Version	XM_005204884.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204884.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG 
TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT 
TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGTCCCT GTTCCAGCCG CTCACTGCAG 
TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA 
CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC 
CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGGACTGAT 
GGACGGCGCT GGTCTTTGGC CTCATTGCCT TCTTCAGGCT ATGGAACCAA CACCCCTAGT 
TCTACCGTGT CATCGTCGTG CTCCTCTCAG GAGAAGCTGC ACCAGTTGCC TTTCCAGCCC 
ACGGCCGATG AGCTGCACTT CTTGACGAAG CATTTCAGCA CAGACAGTGT GCCTGATGAG 
GAGGGGCGGC AGTCCCCCGC CCTGCGGCCC CGCTCCCGCA GCCTCAGTCC TGGACGGTCA 
CCAGTTTCCT TTGACAGTGA AATAATAATG ATGAATCATG TATACAAGGA AAGATTCCCC 
AAGGCCACTG CACAAATGGA AGAGCGTCTG GCCGAGTTCA TCTCCTCCAA TACTCCAGAC 
AACGTGTTGC CCTTGGCTGA TGGAACCTTG AGCTTTATTC ATCATCAGGT GATAGAGATG 
GCCCGAGACT GCCTGGACAA ATCCCGGAGT GGCCTCATTA CCTCACACTA CTTCTACGAA 
CTTCAAGAGA ATTTAGAGAA GCTTCTGCAA GATGCTCATG AGCGCTCAGA GAGTTCAGAA 
GTGGCTTTTG TGATGCAGCT GGTGAAAAAG CTGATGATTG TCATTGCCCG CCCAGCTCGT 
CTCCTGGAAT GCCTGGAGTT TGACCCTGAA GAGTTCTACC ACCTGTTAGA AGCAGCTGAG 
GGCCACGCCA AAGAGGGACA AGGGATTAAA TGTGACATTC CCCGCTACAT CGTTAGCCAA 
CTGGGCCTCA CACGGGATCC CCTAGAGGAA ATGGCCCAGT TGAGCAGCTA TGACAGTCCT 
GATACTCCGG AGACAGATGA TTCAGTCGAG GGCCGTGGGG CATCTCTGAC ATCGAAAAAG 
ACACCCTCTG AGGAGGACTT CGAAACCATT AAGCTCATCA GCAATGGCGC CTATGGGGCC 
GTGTTCCTGG TGCGGCACAA GTCCACCCGG CAGCGCTTTG CCATGAAGAA GATCAACAAG 
CAGAACCTGA TCCTGCGGAA CCAGATCCAG CAGGCCTTCG TGGAGCGGGA CATCCTGACT 
TTTGCTGAGA ACCCCTTTGT GGTCAGCATG TTCTGCTCCT TTGAAACCAA GCGCCACCTG 
TGCATGGTGA TGGAGTATGT GGAAGGAGGA GACTGTGCCA CGCTGCTGAA GAACATCGGG 
GCCCTGCCTG TGGACATGGT GCGCCTGTAC TTTGCAGAAA CTGTGCTAGC CCTGGAGTAC 
TTACACAATT ACGGCATCGT GCACCGTGAC CTCAAGCCTG ACAACCTGCT GATTACATCC 
ATGGGTCACA TCAAGCTCAC TGATTTTGGC CTTTCCAAAA TTGGCCTTAT GAGTCTGACC 
ACAAACTTGT ACGAGGGCCA CATTGAAAAG GATGCACGGG AGTTCCTGGA CAAGCAGGTA 
TGCGGGACCC CGGAGTACAT TGCACCTGAG GTGATCCTGC GGCAGGGCTA CGGGAAGCCA 
GTGGACTGGT GGGCTATGGG CATCATCCTG TACGAGTTCC TGGTGGGCTG CGTCCCTTTC 
TTTGGAGACA CTCCGGAGGA GCTCTTTGGG CAAGTGATCA GTGATGAGAT TGTGTGGCCT 
GAGGGGGACG ATGCACTGCC GCCGGACGCC CAAGATCTCA CCTCAAAACT GCTCCACCAG 
AACCCATTGG AGAGACTGGG CACAGGCAGT GCCTATGAGG TGAAGCAGCA CCCATTCTTC 
ACTGGTCTGG ACTGGACGGG ACTTCTTCGC CAGAAGGCTG AATTTATTCC TCAGCTGGAG 
TCAGAGGATG ATACCAGCTA CTTTGACACC CGCTCAGAGC GATACCACCA CGTGGACTCG 
GAGGATGAGG AGGAAGTGAG TGAGGATGGC TGCCTTGAGA TTCGCCAGTT CTCGTCCTGT 
TCTCCAAGGT TCAGCAAGGT GTACAGCAGC ATGGAGCGGC TCTCGCTGCT CGAGGAGCGC 
CGGACGCCAC CCCCCACCAA GCGCAGCCTG AGTGAGGAAA AGGAGGACCG CACGGATGGC 
CTGGCGGGGC TGAGGGGCCG AGAGCGAAGC TGGGTGATCG GCTCCCCTGA GATATTACGG 
AAGCGGCTGT CGGTGTCCGA GTCGTCCCAC ACGGAAAGCG ATTCCAGCCC TCCACTCACC 
GTGCGGCGCC ACTGCTCGGG CCTCCTGGAC GTGCCTCGCT TCCCTGAGAG CACTGAGGAG 
GCTGTTGGCG CTCCCAGGAG GCAGCAGCAG GAGGGTGCAT GGCTTCTGAC CCCGTCTGGA 
GAGGGGGCGT CTGGGCCTGC CCTGGAACGG CCGGCCGAGC GGCGGCTGAA GCTGAATGAG 
GAACCTGCTG GCCAGAGCAG CGCCCCCAGC CCAGCCATGG AGACCCGAGG AGGCCGCGGA 
ACCCCACAGC TGGCAGAAGG AGCCACAGCC AGGGCCATCA GCGACCTGGC GGTGCGCAGG 
GCCCGCCACC GGCTGCTCTC TGGGGACTCC ATAGAAAAGC GCACCGCTCG CCCCATCAAC 
AAAGTGATCA AGTCCGCCTC AGCCACAGCC CTGTCCCTTC TCATTCCTGC TGAGCACCAC 
TCCTGCTCTC CACTGGCCAG CCCCATGTCC CCACATTCCC AGTCATCCAA CCCATCATCC 
AGGGACTCTT CTCCAAGCAG GGACTTCCTG CCAGCCCTCA GCAGCTCACG GCCCCCCATT 
GTCATCCACC GAGCCGGCAA GAAGTATGGC TTCACCCTAC GAGCCATCCG TGTCTACATG 
GGTGACTCAG ATGTCTACAC CGTGCACCAC ATGGTGTGGC ACGTGGAGGA CGGAGGTCCA 
GCCAGTGAGG CAGGGCTGCG TCAGGGGGAC CTCATCACGC ACGTCAACGG GGAGCCTGTG 
CACGGGCTGG TGCACACGGA GGTGGTGGAG CTGATCCTGA AGAGTGGAAA CAAGGTGTCT 
ATTTCAACAA CTCCCCTGGA GAACACCTCC ATTAAGGTGG GGCCCGCTCG GAAGGGCAGC 
TACAAGGCCA AGATGGCCCG AAGGAGCAAG CGGAGCCGGG GCAAGGATGG ACAAGAAAGC 
AGAAAAAGGA GCTCCCTGTT CCGGAAGATC ACCAAGCAGG CATCCCTGCT CCACACCAGC 
CGCAGCCTTT CTTCCCTTAA CCGCTCCCTG TCGTCCGGCG AGAGTGGGCC AGGCTCGCCC 
ACACACAGCC ACAGCCTTTC TCCCCGCTCT CCCACGCAGG GCTACCGGGT GACCCCTGAA 
GCCGTGCACT CAGTGGGGGG GAATTCATCA CAGAGCAGCT CCCCCAGCTC CAGCGTGCCC 
AGTTCTCCTG CCGGCTCTGG GCACACACGG CCGAGCTCCC TCCACGGTCT GGCGCCCAAG 
CTCCAACGCC AGTACCGCTC TCCACGGCGC AAGTCAGCAG GCAGCATCCC GCTGTCACCA 
CTGGCCCACA CCCCTTCACC ACCACCAGCG GCTTCGCCTC AGCGTTCCCC ATCACCTCTG 
TCTGGCCACG GGGCCCAGGC CTTCCCTACC AAGCTCCACT TGTCGCCTCC CCTAGGCCGG 
CAACTCTCAC GGCCCAAGAG CGCGGAGCCA CCCCGCTCAC CACTACTTAA GAGGGTGCAG 
TCAGCTGAAA AACTGGCAGC TGCACTGGCT GCTTCTGAGA AGAAGCTCGC CACTTCTCGA 
AAACAGAGCC TTGACCTGCC CCACCCCGAG CTAAAGAAGG AATTGCCGCC CAGGGAAGTC 
AGCTCTCTGG AGGTAGTTGG GACCAGGAGT GTGCTGTCTG GAAAGGGGGC GCTGCCAGGG 
AAAGGGGTGT TGCAGGCTGC TCCCTCACGG GCCTTAGGGA CCCTCCGCCA GGACAGGGCT 
GAACGGCGAG AGTCGCTGCA GAAGCAGGAA GCCATCCGGG AGGTTGACTC TTCAGAAGAT 
GATACTGATG AGGGGCCTGA GAACAGCCAA GGTGTTCAGG AGCCAAGCTT GGTACCCCAC 
CGAGAAGGGG GCCAGGATCC ACCCCTCAGA GGAGCAGGAG AGGGTGAGGA AGAGGATGCC 
TTCTTGCCCA GGGATCCCAG GGGACAGGGC CCAGTGGTCA CAGGTCTACT GACAGGGGTC 
ACACTGGGAT CTCCCAGAAT GGAAGGCCCT GGTGTCCCCC AGAAGAAGCT TGGAATCCTA 
CAGGCTTTTG AAGAGGGTTC CAGCTCCTCG TCCTCAGCCC CCAACCTGGG GAAGGCTGGC 
CCCACAGACC CCATCCCCGC TGAAGGCTGC TGGAAGGCCC AGCACCTCCA CACCCAGGCA 
CTGACAGCAC TTTGTCCGAG CTCATCAGGA CGCATCCCTG CCAGTTGCTC CACTGCCTCC 
ACCCCTGGAG AGCTGGGCCC ATGGTCCTGG AAATTCCTCT TCAAGGGCCC TGACAGGGCA 
TACCCAACCG GAAAGGCAGC AATGGAAGAT GGGCCAGCCA GCTCCCAAGA TTTGGAAACT 
ACAACTCCAG GCCACCCTGC AAACCTGTCC CCCAGACAGG AGGGGAAGTC ATGGCCACCC 
AGTGCCCCTG GGCTGGCCCA TCCACCTTGT GAGGTTCCCA GCCAGAGCTG GCTATGGGAG 
TCTGAGTGTG CACAGAGAGA GAAAGAGGAG CCAGCGCTGG GCATCACCAA AGTGCCTGAT 
GCCTCAGGTG ACAGGAGGCA GGACGTTCCA TGCAAGAGCT GCCCCTTCAC CCCAGAGCCT 
GGGCCCAGCC TGCTCCAGAA GGGCCAAGAC CCAGGGGGCC CTCAGGAGCA TCAGGACTTG 
GCATTGTTAC CTGATGAGCT TTTAAAGCAA ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204941.1
CDS	244..5379
Translation	MMKRRRERLGAPCLRIQISALCRGSEVNRHVLSPTSAPAWSLSKVPFSAECTLATSPLAFFLNTHAHSSPGSPCSSRSLQWSCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_005204884.4
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204884.4

ORF Insert Sequence:

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG 
TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT 
TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGTCCCT GTTCCAGCCG CTCACTGCAG 
TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA 
CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC 
CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGGACTGAT 
GGACGGCGCT GGTCTTTGGC CTCATTGCCT TCTTCAGGCT ATGGAACCAA CACCCCTAGT 
TCTACCGTGT CATCGTCGTG CTCCTCTCAG GAGAAGCTGC ACCAGTTGCC TTTCCAGCCC 
ACGGCCGATG AGCTGCACTT CTTGACGAAG CATTTCAGCA CAGACAGTGT GCCTGATGAG 
GAGGGGCGGC AGTCCCCCGC CCTGCGGCCC CGCTCCCGCA GCCTCAGTCC TGGACGGTCA 
CCAGTTTCCT TTGACAGTGA AATAATAATG ATGAATCATG TATACAAGGA AAGATTCCCC 
AAGGCCACTG CACAAATGGA AGAGCGTCTG GCCGAGTTCA TCTCCTCCAA TACTCCAGAC 
AACGTGTTGC CCTTGGCTGA TGGAACCTTG AGCTTTATTC ATCATCAGGT GATAGAGATG 
GCCCGAGACT GCCTGGACAA ATCCCGGAGT GGCCTCATTA CCTCACACTA CTTCTACGAA 
CTTCAAGAGA ATTTAGAGAA GCTTCTGCAA GATGCTCATG AGCGCTCAGA GAGTTCAGAA 
GTGGCTTTTG TGATGCAGCT GGTGAAAAAG CTGATGATTG TCATTGCCCG CCCAGCTCGT 
CTCCTGGAAT GCCTGGAGTT TGACCCTGAA GAGTTCTACC ACCTGTTAGA AGCAGCTGAG 
GGCCACGCCA AAGAGGGACA AGGGATTAAA TGTGACATTC CCCGCTACAT CGTTAGCCAA 
CTGGGCCTCA CACGGGATCC CCTAGAGGAA ATGGCCCAGT TGAGCAGCTA TGACAGTCCT 
GATACTCCGG AGACAGATGA TTCAGTCGAG GGCCGTGGGG CATCTCTGAC ATCGAAAAAG 
ACACCCTCTG AGGAGGACTT CGAAACCATT AAGCTCATCA GCAATGGCGC CTATGGGGCC 
GTGTTCCTGG TGCGGCACAA GTCCACCCGG CAGCGCTTTG CCATGAAGAA GATCAACAAG 
CAGAACCTGA TCCTGCGGAA CCAGATCCAG CAGGCCTTCG TGGAGCGGGA CATCCTGACT 
TTTGCTGAGA ACCCCTTTGT GGTCAGCATG TTCTGCTCCT TTGAAACCAA GCGCCACCTG 
TGCATGGTGA TGGAGTATGT GGAAGGAGGA GACTGTGCCA CGCTGCTGAA GAACATCGGG 
GCCCTGCCTG TGGACATGGT GCGCCTGTAC TTTGCAGAAA CTGTGCTAGC CCTGGAGTAC 
TTACACAATT ACGGCATCGT GCACCGTGAC CTCAAGCCTG ACAACCTGCT GATTACATCC 
ATGGGTCACA TCAAGCTCAC TGATTTTGGC CTTTCCAAAA TTGGCCTTAT GAGTCTGACC 
ACAAACTTGT ACGAGGGCCA CATTGAAAAG GATGCACGGG AGTTCCTGGA CAAGCAGGTA 
TGCGGGACCC CGGAGTACAT TGCACCTGAG GTGATCCTGC GGCAGGGCTA CGGGAAGCCA 
GTGGACTGGT GGGCTATGGG CATCATCCTG TACGAGTTCC TGGTGGGCTG CGTCCCTTTC 
TTTGGAGACA CTCCGGAGGA GCTCTTTGGG CAAGTGATCA GTGATGAGAT TGTGTGGCCT 
GAGGGGGACG ATGCACTGCC GCCGGACGCC CAAGATCTCA CCTCAAAACT GCTCCACCAG 
AACCCATTGG AGAGACTGGG CACAGGCAGT GCCTATGAGG TGAAGCAGCA CCCATTCTTC 
ACTGGTCTGG ACTGGACGGG ACTTCTTCGC CAGAAGGCTG AATTTATTCC TCAGCTGGAG 
TCAGAGGATG ATACCAGCTA CTTTGACACC CGCTCAGAGC GATACCACCA CGTGGACTCG 
GAGGATGAGG AGGAAGTGAG TGAGGATGGC TGCCTTGAGA TTCGCCAGTT CTCGTCCTGT 
TCTCCAAGGT TCAGCAAGGT GTACAGCAGC ATGGAGCGGC TCTCGCTGCT CGAGGAGCGC 
CGGACGCCAC CCCCCACCAA GCGCAGCCTG AGTGAGGAAA AGGAGGACCG CACGGATGGC 
CTGGCGGGGC TGAGGGGCCG AGAGCGAAGC TGGGTGATCG GCTCCCCTGA GATATTACGG 
AAGCGGCTGT CGGTGTCCGA GTCGTCCCAC ACGGAAAGCG ATTCCAGCCC TCCACTCACC 
GTGCGGCGCC ACTGCTCGGG CCTCCTGGAC GTGCCTCGCT TCCCTGAGAG CACTGAGGAG 
GCTGTTGGCG CTCCCAGGAG GCAGCAGCAG GAGGGTGCAT GGCTTCTGAC CCCGTCTGGA 
GAGGGGGCGT CTGGGCCTGC CCTGGAACGG CCGGCCGAGC GGCGGCTGAA GCTGAATGAG 
GAACCTGCTG GCCAGAGCAG CGCCCCCAGC CCAGCCATGG AGACCCGAGG AGGCCGCGGA 
ACCCCACAGC TGGCAGAAGG AGCCACAGCC AGGGCCATCA GCGACCTGGC GGTGCGCAGG 
GCCCGCCACC GGCTGCTCTC TGGGGACTCC ATAGAAAAGC GCACCGCTCG CCCCATCAAC 
AAAGTGATCA AGTCCGCCTC AGCCACAGCC CTGTCCCTTC TCATTCCTGC TGAGCACCAC 
TCCTGCTCTC CACTGGCCAG CCCCATGTCC CCACATTCCC AGTCATCCAA CCCATCATCC 
AGGGACTCTT CTCCAAGCAG GGACTTCCTG CCAGCCCTCA GCAGCTCACG GCCCCCCATT 
GTCATCCACC GAGCCGGCAA GAAGTATGGC TTCACCCTAC GAGCCATCCG TGTCTACATG 
GGTGACTCAG ATGTCTACAC CGTGCACCAC ATGGTGTGGC ACGTGGAGGA CGGAGGTCCA 
GCCAGTGAGG CAGGGCTGCG TCAGGGGGAC CTCATCACGC ACGTCAACGG GGAGCCTGTG 
CACGGGCTGG TGCACACGGA GGTGGTGGAG CTGATCCTGA AGAGTGGAAA CAAGGTGTCT 
ATTTCAACAA CTCCCCTGGA GAACACCTCC ATTAAGGTGG GGCCCGCTCG GAAGGGCAGC 
TACAAGGCCA AGATGGCCCG AAGGAGCAAG CGGAGCCGGG GCAAGGATGG ACAAGAAAGC 
AGAAAAAGGA GCTCCCTGTT CCGGAAGATC ACCAAGCAGG CATCCCTGCT CCACACCAGC 
CGCAGCCTTT CTTCCCTTAA CCGCTCCCTG TCGTCCGGCG AGAGTGGGCC AGGCTCGCCC 
ACACACAGCC ACAGCCTTTC TCCCCGCTCT CCCACGCAGG GCTACCGGGT GACCCCTGAA 
GCCGTGCACT CAGTGGGGGG GAATTCATCA CAGAGCAGCT CCCCCAGCTC CAGCGTGCCC 
AGTTCTCCTG CCGGCTCTGG GCACACACGG CCGAGCTCCC TCCACGGTCT GGCGCCCAAG 
CTCCAACGCC AGTACCGCTC TCCACGGCGC AAGTCAGCAG GCAGCATCCC GCTGTCACCA 
CTGGCCCACA CCCCTTCACC ACCACCAGCG GCTTCGCCTC AGCGTTCCCC ATCACCTCTG 
TCTGGCCACG GGGCCCAGGC CTTCCCTACC AAGCTCCACT TGTCGCCTCC CCTAGGCCGG 
CAACTCTCAC GGCCCAAGAG CGCGGAGCCA CCCCGCTCAC CACTACTTAA GAGGGTGCAG 
TCAGCTGAAA AACTGGCAGC TGCACTGGCT GCTTCTGAGA AGAAGCTCGC CACTTCTCGA 
AAACAGAGCC TTGACCTGCC CCACCCCGAG CTAAAGAAGG AATTGCCGCC CAGGGAAGTC 
AGCTCTCTGG AGGTAGTTGG GACCAGGAGT GTGCTGTCTG GAAAGGGGGC GCTGCCAGGG 
AAAGGGGTGT TGCAGGCTGC TCCCTCACGG GCCTTAGGGA CCCTCCGCCA GGACAGGGCT 
GAACGGCGAG AGTCGCTGCA GAAGCAGGAA GCCATCCGGG AGGTTGACTC TTCAGAAGAT 
GATACTGATG AGGGGCCTGA GAACAGCCAA GGTGTTCAGG AGCCAAGCTT GGTACCCCAC 
CGAGAAGGGG GCCAGGATCC ACCCCTCAGA GGAGCAGGAG AGGGTGAGGA AGAGGATGCC 
TTCTTGCCCA GGGATCCCAG GGGACAGGGC CCAGTGGTCA CAGGTCTACT GACAGGGGTC 
ACACTGGGAT CTCCCAGAAT GGAAGGCCCT GGTGTCCCCC AGAAGAAGCT TGGAATCCTA 
CAGGCTTTTG AAGAGGGTTC CAGCTCCTCG TCCTCAGCCC CCAACCTGGG GAAGGCTGGC 
CCCACAGACC CCATCCCCGC TGAAGGCTGC TGGAAGGCCC AGCACCTCCA CACCCAGGCA 
CTGACAGCAC TTTGTCCGAG CTCATCAGGA CGCATCCCTG CCAGTTGCTC CACTGCCTCC 
ACCCCTGGAG AGCTGGGCCC ATGGTCCTGG AAATTCCTCT TCAAGGGCCC TGACAGGGCA 
TACCCAACCG GAAAGGCAGC AATGGAAGAT GGGCCAGCCA GCTCCCAAGA TTTGGAAACT 
ACAACTCCAG GCCACCCTGC AAACCTGTCC CCCAGACAGG AGGGGAAGTC ATGGCCACCC 
AGTGCCCCTG GGCTGGCCCA TCCACCTTGT GAGGTTCCCA GCCAGAGCTG GCTATGGGAG 
TCTGAGTGTG CACAGAGAGA GAAAGAGGAG CCAGCGCTGG GCATCACCAA AGTGCCTGAT 
GCCTCAGGTG ACAGGAGGCA GGACGTTCCA TGCAAGAGCT GCCCCTTCAC CCCAGAGCCT 
GGGCCCAGCC TGCTCCAGAA GGGCCAAGAC CCAGGGGGCC CTCAGGAGCA TCAGGACTTG 
GCATTGTTAC CTGATGAGCT TTTAAAGCAA ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_024990152.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845920.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA.

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CloneID	OBa53214
Clone ID Related Accession (Same CDS sequence)	XM_024990152.1
Accession Version	XM_024990152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGATTC TCAAGGATGT GGTAACTGGA CGTAGTCCGC TGCTCTTCAG GAAGCTCAGT 
AATCCTGACA TATTTTCATC CACTGGAAAA GCTAAACTTC AACGACAACT TAGTCAGGAT 
GACTGTAAGT TACGGAGAGG AAGCCTGGCC AGTTCTTTGT CAGGCAAGCA GCTGCTCCCC 
TTGTCCGGCA GTGCACACAG CAGCGTGGGA CAGGTGACTT GGCAGTCTTC AGGAGAAGCG 
TCCAACCTGG TGCGGATGAG GAATCAGTCC CTCGGACAGT CTGCGCCTTC TCTTACCGCT 
GGCTTGAAGG AGCTGAGCCT TCCAAGAAGA GGCAGCTTCT GTCGGACAAG TAACCGCAAG 
AGTTTGATTG TGACCTCTAG TACGTCACCT ACACTGCCAC GGCCACACTC ACCACTCCAT 
GGCCACACAG GTAACAGTCC TTTGGATAGT CCCCGGAATT TCTCTCCAAA TGCACCTGCT 
CACTTTTCCT TTGTTCCTGC CCGTAGCCAT GGCCACAGAG CAGACAGGAC TGATGGACGG 
CGCTGGTCTT TGGCCTCATT GCCTTCTTCA GGCTATGGAA CCAACACCCC TAGTTCTACC 
GTGTCATCGT CGTGCTCCTC TCAGGAGAAG CTGCACCAGT TGCCTTTCCA GCCCACGGCC 
GATGAGCTGC ACTTCTTGAC GAAGCATTTC AGCACAGACA GTGTGCCTGA TGAGGAGGGG 
CGGCAGTCCC CCGCCCTGCG GCCCCGCTCC CGCAGCCTCA GTCCTGGACG GTCACCAGTT 
TCCTTTGACA GTGAAATAAT AATGATGAAT CATGTATACA AGGAAAGATT CCCCAAGGCC 
ACTGCACAAA TGGAAGAGCG TCTGGCCGAG TTCATCTCCT CCAATACTCC AGACAACGTG 
TTGCCCTTGG CTGATGGAAC CTTGAGCTTT ATTCATCATC AGGTGATAGA GATGGCCCGA 
GACTGCCTGG ACAAATCCCG GAGTGGCCTC ATTACCTCAC ACTACTTCTA CGAACTTCAA 
GAGAATTTAG AGAAGCTTCT GCAAGATGCT CATGAGCGCT CAGAGAGTTC AGAAGTGGCT 
TTTGTGATGC AGCTGGTGAA AAAGCTGATG ATTGTCATTG CCCGCCCAGC TCGTCTCCTG 
GAATGCCTGG AGTTTGACCC TGAAGAGTTC TACCACCTGT TAGAAGCAGC TGAGGGCCAC 
GCCAAAGAGG GACAAGGGAT TAAATGTGAC ATTCCCCGCT ACATCGTTAG CCAACTGGGC 
CTCACACGGG ATCCCCTAGA GGAAATGGCC CAGTTGAGCA GCTATGACAG TCCTGATACT 
CCGGAGACAG ATGATTCAGT CGAGGGCCGT GGGGCATCTC TGACATCGAA AAAGACACCC 
TCTGAGGAGG ACTTCGAAAC CATTAAGCTC ATCAGCAATG GCGCCTATGG GGCCGTGTTC 
CTGGTGCGGC ACAAGTCCAC CCGGCAGCGC TTTGCCATGA AGAAGATCAA CAAGCAGAAC 
CTGATCCTGC GGAACCAGAT CCAGCAGGCC TTCGTGGAGC GGGACATCCT GACTTTTGCT 
GAGAACCCCT TTGTGGTCAG CATGTTCTGC TCCTTTGAAA CCAAGCGCCA CCTGTGCATG 
GTGATGGAGT ATGTGGAAGG AGGAGACTGT GCCACGCTGC TGAAGAACAT CGGGGCCCTG 
CCTGTGGACA TGGTGCGCCT GTACTTTGCA GAAACTGTGC TAGCCCTGGA GTACTTACAC 
AATTACGGCA TCGTGCACCG TGACCTCAAG CCTGACAACC TGCTGATTAC ATCCATGGGT 
CACATCAAGC TCACTGATTT TGGCCTTTCC AAAATTGGCC TTATGAGTCT GACCACAAAC 
TTGTACGAGG GCCACATTGA AAAGGATGCA CGGGAGTTCC TGGACAAGCA GGTATGCGGG 
ACCCCGGAGT ACATTGCACC TGAGGTGATC CTGCGGCAGG GCTACGGGAA GCCAGTGGAC 
TGGTGGGCTA TGGGCATCAT CCTGTACGAG TTCCTGGTGG GCTGCGTCCC TTTCTTTGGA 
GACACTCCGG AGGAGCTCTT TGGGCAAGTG ATCAGTGATG AGATTGTGTG GCCTGAGGGG 
GACGATGCAC TGCCGCCGGA CGCCCAAGAT CTCACCTCAA AACTGCTCCA CCAGAACCCA 
TTGGAGAGAC TGGGCACAGG CAGTGCCTAT GAGGTGAAGC AGCACCCATT CTTCACTGGT 
CTGGACTGGA CGGGACTTCT TCGCCAGAAG GCTGAATTTA TTCCTCAGCT GGAGTCAGAG 
GATGATACCA GCTACTTTGA CACCCGCTCA GAGCGATACC ACCACGTGGA CTCGGAGGAT 
GAGGAGGAAG TGAGTGAGGA TGGCTGCCTT GAGATTCGCC AGTTCTCGTC CTGTTCTCCA 
AGGTTCAGCA AGGTGTACAG CAGCATGGAG CGGCTCTCGC TGCTCGAGGA GCGCCGGACG 
CCACCCCCCA CCAAGCGCAG CCTGAGTGAG GAAAAGGAGG ACCGCACGGA TGGCCTGGCG 
GGGCTGAGGG GCCGAGAGCG AAGCTGGGTG ATCGGCTCCC CTGAGATATT ACGGAAGCGG 
CTGTCGGTGT CCGAGTCGTC CCACACGGAA AGCGATTCCA GCCCTCCACT CACCGTGCGG 
CGCCACTGCT CGGGCCTCCT GGACGTGCCT CGCTTCCCTG AGAGCACTGA GGAGGCTGTT 
GGCGCTCCCA GGAGGCAGCA GCAGGAGGGT GCATGGCTTC TGACCCCGTC TGGAGAGGGG 
GCGTCTGGGC CTGCCCTGGA ACGGCCGGCC GAGCGGCGGC TGAAGCTGAA TGAGGAACCT 
GCTGGCCAGA GCAGCGCCCC CAGCCCAGCC ATGGAGACCC GAGGAGGCCG CGGAACCCCA 
CAGCTGGCAG AAGGAGCCAC AGCCAGGGCC ATCAGCGACC TGGCGGTGCG CAGGGCCCGC 
CACCGGCTGC TCTCTGGGGA CTCCATAGAA AAGCGCACCG CTCGCCCCAT CAACAAAGTG 
ATCAAGTCCG CCTCAGCCAC AGCCCTGTCC CTTCTCATTC CTGCTGAGCA CCACTCCTGC 
TCTCCACTGG CCAGCCCCAT GTCCCCACAT TCCCAGTCAT CCAACCCATC ATCCAGGGAC 
TCTTCTCCAA GCAGGGACTT CCTGCCAGCC CTCAGCAGCT CACGGCCCCC CATTGTCATC 
CACCGAGCCG GCAAGAAGTA TGGCTTCACC CTACGAGCCA TCCGTGTCTA CATGGGTGAC 
TCAGATGTCT ACACCGTGCA CCACATGGTG TGGCACGTGG AGGACGGAGG TCCAGCCAGT 
GAGGCAGGGC TGCGTCAGGG GGACCTCATC ACGCACGTCA ACGGGGAGCC TGTGCACGGG 
CTGGTGCACA CGGAGGTGGT GGAGCTGATC CTGAAGAGTG GAAACAAGGT GTCTATTTCA 
ACAACTCCCC TGGAGAACAC CTCCATTAAG GTGGGGCCCG CTCGGAAGGG CAGCTACAAG 
GCCAAGATGG CCCGAAGGAG CAAGCGGAGC CGGGGCAAGG ATGGACAAGA AAGCAGAAAA 
AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC 
CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC 
AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG 
CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT 
CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA 
CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC 
CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC 
CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC 
TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT 
GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG 
AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT 
CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG 
GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG 
CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT 
GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA 
GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG 
CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG 
GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT 
TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA 
GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA 
GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT 
GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA 
ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT 
CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC 
CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG 
TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA 
GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC 
AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG 
TTACCTGATG AGCTTTTAAA GCAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845920.1
CDS	16..5265
Translation	MGILKDVVTGRSPLLFRKLSNPDIFSSTGKAKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSGSAHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_024990152.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990152.1

ORF Insert Sequence:

ATGGGGATTC TCAAGGATGT GGTAACTGGA CGTAGTCCGC TGCTCTTCAG GAAGCTCAGT 
AATCCTGACA TATTTTCATC CACTGGAAAA GCTAAACTTC AACGACAACT TAGTCAGGAT 
GACTGTAAGT TACGGAGAGG AAGCCTGGCC AGTTCTTTGT CAGGCAAGCA GCTGCTCCCC 
TTGTCCGGCA GTGCACACAG CAGCGTGGGA CAGGTGACTT GGCAGTCTTC AGGAGAAGCG 
TCCAACCTGG TGCGGATGAG GAATCAGTCC CTCGGACAGT CTGCGCCTTC TCTTACCGCT 
GGCTTGAAGG AGCTGAGCCT TCCAAGAAGA GGCAGCTTCT GTCGGACAAG TAACCGCAAG 
AGTTTGATTG TGACCTCTAG TACGTCACCT ACACTGCCAC GGCCACACTC ACCACTCCAT 
GGCCACACAG GTAACAGTCC TTTGGATAGT CCCCGGAATT TCTCTCCAAA TGCACCTGCT 
CACTTTTCCT TTGTTCCTGC CCGTAGCCAT GGCCACAGAG CAGACAGGAC TGATGGACGG 
CGCTGGTCTT TGGCCTCATT GCCTTCTTCA GGCTATGGAA CCAACACCCC TAGTTCTACC 
GTGTCATCGT CGTGCTCCTC TCAGGAGAAG CTGCACCAGT TGCCTTTCCA GCCCACGGCC 
GATGAGCTGC ACTTCTTGAC GAAGCATTTC AGCACAGACA GTGTGCCTGA TGAGGAGGGG 
CGGCAGTCCC CCGCCCTGCG GCCCCGCTCC CGCAGCCTCA GTCCTGGACG GTCACCAGTT 
TCCTTTGACA GTGAAATAAT AATGATGAAT CATGTATACA AGGAAAGATT CCCCAAGGCC 
ACTGCACAAA TGGAAGAGCG TCTGGCCGAG TTCATCTCCT CCAATACTCC AGACAACGTG 
TTGCCCTTGG CTGATGGAAC CTTGAGCTTT ATTCATCATC AGGTGATAGA GATGGCCCGA 
GACTGCCTGG ACAAATCCCG GAGTGGCCTC ATTACCTCAC ACTACTTCTA CGAACTTCAA 
GAGAATTTAG AGAAGCTTCT GCAAGATGCT CATGAGCGCT CAGAGAGTTC AGAAGTGGCT 
TTTGTGATGC AGCTGGTGAA AAAGCTGATG ATTGTCATTG CCCGCCCAGC TCGTCTCCTG 
GAATGCCTGG AGTTTGACCC TGAAGAGTTC TACCACCTGT TAGAAGCAGC TGAGGGCCAC 
GCCAAAGAGG GACAAGGGAT TAAATGTGAC ATTCCCCGCT ACATCGTTAG CCAACTGGGC 
CTCACACGGG ATCCCCTAGA GGAAATGGCC CAGTTGAGCA GCTATGACAG TCCTGATACT 
CCGGAGACAG ATGATTCAGT CGAGGGCCGT GGGGCATCTC TGACATCGAA AAAGACACCC 
TCTGAGGAGG ACTTCGAAAC CATTAAGCTC ATCAGCAATG GCGCCTATGG GGCCGTGTTC 
CTGGTGCGGC ACAAGTCCAC CCGGCAGCGC TTTGCCATGA AGAAGATCAA CAAGCAGAAC 
CTGATCCTGC GGAACCAGAT CCAGCAGGCC TTCGTGGAGC GGGACATCCT GACTTTTGCT 
GAGAACCCCT TTGTGGTCAG CATGTTCTGC TCCTTTGAAA CCAAGCGCCA CCTGTGCATG 
GTGATGGAGT ATGTGGAAGG AGGAGACTGT GCCACGCTGC TGAAGAACAT CGGGGCCCTG 
CCTGTGGACA TGGTGCGCCT GTACTTTGCA GAAACTGTGC TAGCCCTGGA GTACTTACAC 
AATTACGGCA TCGTGCACCG TGACCTCAAG CCTGACAACC TGCTGATTAC ATCCATGGGT 
CACATCAAGC TCACTGATTT TGGCCTTTCC AAAATTGGCC TTATGAGTCT GACCACAAAC 
TTGTACGAGG GCCACATTGA AAAGGATGCA CGGGAGTTCC TGGACAAGCA GGTATGCGGG 
ACCCCGGAGT ACATTGCACC TGAGGTGATC CTGCGGCAGG GCTACGGGAA GCCAGTGGAC 
TGGTGGGCTA TGGGCATCAT CCTGTACGAG TTCCTGGTGG GCTGCGTCCC TTTCTTTGGA 
GACACTCCGG AGGAGCTCTT TGGGCAAGTG ATCAGTGATG AGATTGTGTG GCCTGAGGGG 
GACGATGCAC TGCCGCCGGA CGCCCAAGAT CTCACCTCAA AACTGCTCCA CCAGAACCCA 
TTGGAGAGAC TGGGCACAGG CAGTGCCTAT GAGGTGAAGC AGCACCCATT CTTCACTGGT 
CTGGACTGGA CGGGACTTCT TCGCCAGAAG GCTGAATTTA TTCCTCAGCT GGAGTCAGAG 
GATGATACCA GCTACTTTGA CACCCGCTCA GAGCGATACC ACCACGTGGA CTCGGAGGAT 
GAGGAGGAAG TGAGTGAGGA TGGCTGCCTT GAGATTCGCC AGTTCTCGTC CTGTTCTCCA 
AGGTTCAGCA AGGTGTACAG CAGCATGGAG CGGCTCTCGC TGCTCGAGGA GCGCCGGACG 
CCACCCCCCA CCAAGCGCAG CCTGAGTGAG GAAAAGGAGG ACCGCACGGA TGGCCTGGCG 
GGGCTGAGGG GCCGAGAGCG AAGCTGGGTG ATCGGCTCCC CTGAGATATT ACGGAAGCGG 
CTGTCGGTGT CCGAGTCGTC CCACACGGAA AGCGATTCCA GCCCTCCACT CACCGTGCGG 
CGCCACTGCT CGGGCCTCCT GGACGTGCCT CGCTTCCCTG AGAGCACTGA GGAGGCTGTT 
GGCGCTCCCA GGAGGCAGCA GCAGGAGGGT GCATGGCTTC TGACCCCGTC TGGAGAGGGG 
GCGTCTGGGC CTGCCCTGGA ACGGCCGGCC GAGCGGCGGC TGAAGCTGAA TGAGGAACCT 
GCTGGCCAGA GCAGCGCCCC CAGCCCAGCC ATGGAGACCC GAGGAGGCCG CGGAACCCCA 
CAGCTGGCAG AAGGAGCCAC AGCCAGGGCC ATCAGCGACC TGGCGGTGCG CAGGGCCCGC 
CACCGGCTGC TCTCTGGGGA CTCCATAGAA AAGCGCACCG CTCGCCCCAT CAACAAAGTG 
ATCAAGTCCG CCTCAGCCAC AGCCCTGTCC CTTCTCATTC CTGCTGAGCA CCACTCCTGC 
TCTCCACTGG CCAGCCCCAT GTCCCCACAT TCCCAGTCAT CCAACCCATC ATCCAGGGAC 
TCTTCTCCAA GCAGGGACTT CCTGCCAGCC CTCAGCAGCT CACGGCCCCC CATTGTCATC 
CACCGAGCCG GCAAGAAGTA TGGCTTCACC CTACGAGCCA TCCGTGTCTA CATGGGTGAC 
TCAGATGTCT ACACCGTGCA CCACATGGTG TGGCACGTGG AGGACGGAGG TCCAGCCAGT 
GAGGCAGGGC TGCGTCAGGG GGACCTCATC ACGCACGTCA ACGGGGAGCC TGTGCACGGG 
CTGGTGCACA CGGAGGTGGT GGAGCTGATC CTGAAGAGTG GAAACAAGGT GTCTATTTCA 
ACAACTCCCC TGGAGAACAC CTCCATTAAG GTGGGGCCCG CTCGGAAGGG CAGCTACAAG 
GCCAAGATGG CCCGAAGGAG CAAGCGGAGC CGGGGCAAGG ATGGACAAGA AAGCAGAAAA 
AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC 
CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC 
AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG 
CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT 
CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA 
CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC 
CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC 
CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC 
TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT 
GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG 
AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT 
CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG 
GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG 
CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT 
GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA 
GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG 
CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG 
GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT 
TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA 
GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA 
GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT 
GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA 
ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT 
CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC 
CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG 
TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA 
GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC 
AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG 
TTACCTGATG AGCTTTTAAA GCAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_005204883.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204940.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA.

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CloneID	OBa18162
Clone ID Related Accession (Same CDS sequence)	XM_005204883.3 , XM_015462788.1
Accession Version	XM_005204883.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204883.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG 
TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT 
TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG 
TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA 
CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC 
CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGCCATGGC 
CACAGAGCAG ACAGGACTGA TGGACGGCGC TGGTCTTTGG CCTCATTGCC TTCTTCAGGC 
TATGGAACCA ACACCCCTAG TTCTACCGTG TCATCGTCGT GCTCCTCTCA GGAGAAGCTG 
CACCAGTTGC CTTTCCAGCC CACGGCCGAT GAGCTGCACT TCTTGACGAA GCATTTCAGC 
ACAGACAGTG TGCCTGATGA GGAGGGGCGG CAGTCCCCCG CCCTGCGGCC CCGCTCCCGC 
AGCCTCAGTC CTGGACGGTC ACCAGTTTCC TTTGACAGTG AAATAATAAT GATGAATCAT 
GTATACAAGG AAAGATTCCC CAAGGCCACT GCACAAATGG AAGAGCGTCT GGCCGAGTTC 
ATCTCCTCCA ATACTCCAGA CAACGTGTTG CCCTTGGCTG ATGGAACCTT GAGCTTTATT 
CATCATCAGG TGATAGAGAT GGCCCGAGAC TGCCTGGACA AATCCCGGAG TGGCCTCATT 
ACCTCACACT ACTTCTACGA ACTTCAAGAG AATTTAGAGA AGCTTCTGCA AGATGCTCAT 
GAGCGCTCAG AGAGTTCAGA AGTGGCTTTT GTGATGCAGC TGGTGAAAAA GCTGATGATT 
GTCATTGCCC GCCCAGCTCG TCTCCTGGAA TGCCTGGAGT TTGACCCTGA AGAGTTCTAC 
CACCTGTTAG AAGCAGCTGA GGGCCACGCC AAAGAGGGAC AAGGGATTAA ATGTGACATT 
CCCCGCTACA TCGTTAGCCA ACTGGGCCTC ACACGGGATC CCCTAGAGGA AATGGCCCAG 
TTGAGCAGCT ATGACAGTCC TGATACTCCG GAGACAGATG ATTCAGTCGA GGGCCGTGGG 
GCATCTCTGA CATCGAAAAA GACACCCTCT GAGGAGGACT TCGAAACCAT TAAGCTCATC 
AGCAATGGCG CCTATGGGGC CGTGTTCCTG GTGCGGCACA AGTCCACCCG GCAGCGCTTT 
GCCATGAAGA AGATCAACAA GCAGAACCTG ATCCTGCGGA ACCAGATCCA GCAGGCCTTC 
GTGGAGCGGG ACATCCTGAC TTTTGCTGAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC 
TTTGAAACCA AGCGCCACCT GTGCATGGTG ATGGAGTATG TGGAAGGAGG AGACTGTGCC 
ACGCTGCTGA AGAACATCGG GGCCCTGCCT GTGGACATGG TGCGCCTGTA CTTTGCAGAA 
ACTGTGCTAG CCCTGGAGTA CTTACACAAT TACGGCATCG TGCACCGTGA CCTCAAGCCT 
GACAACCTGC TGATTACATC CATGGGTCAC ATCAAGCTCA CTGATTTTGG CCTTTCCAAA 
ATTGGCCTTA TGAGTCTGAC CACAAACTTG TACGAGGGCC ACATTGAAAA GGATGCACGG 
GAGTTCCTGG ACAAGCAGGT ATGCGGGACC CCGGAGTACA TTGCACCTGA GGTGATCCTG 
CGGCAGGGCT ACGGGAAGCC AGTGGACTGG TGGGCTATGG GCATCATCCT GTACGAGTTC 
CTGGTGGGCT GCGTCCCTTT CTTTGGAGAC ACTCCGGAGG AGCTCTTTGG GCAAGTGATC 
AGTGATGAGA TTGTGTGGCC TGAGGGGGAC GATGCACTGC CGCCGGACGC CCAAGATCTC 
ACCTCAAAAC TGCTCCACCA GAACCCATTG GAGAGACTGG GCACAGGCAG TGCCTATGAG 
GTGAAGCAGC ACCCATTCTT CACTGGTCTG GACTGGACGG GACTTCTTCG CCAGAAGGCT 
GAATTTATTC CTCAGCTGGA GTCAGAGGAT GATACCAGCT ACTTTGACAC CCGCTCAGAG 
CGATACCACC ACGTGGACTC GGAGGATGAG GAGGAAGTGA GTGAGGATGG CTGCCTTGAG 
ATTCGCCAGT TCTCGTCCTG TTCTCCAAGG TTCAGCAAGG TGTACAGCAG CATGGAGCGG 
CTCTCGCTGC TCGAGGAGCG CCGGACGCCA CCCCCCACCA AGCGCAGCCT GAGTGAGGAA 
AAGGAGGACC GCACGGATGG CCTGGCGGGG CTGAGGGGCC GAGAGCGAAG CTGGGTGATC 
GGCTCCCCTG AGATATTACG GAAGCGGCTG TCGGTGTCCG AGTCGTCCCA CACGGAAAGC 
GATTCCAGCC CTCCACTCAC CGTGCGGCGC CACTGCTCGG GCCTCCTGGA CGTGCCTCGC 
TTCCCTGAGA GCACTGAGGA GGCTGTTGGC GCTCCCAGGA GGCAGCAGCA GGAGGGTGCA 
TGGCTTCTGA CCCCGTCTGG AGAGGGGGCG TCTGGGCCTG CCCTGGAACG GCCGGCCGAG 
CGGCGGCTGA AGCTGAATGA GGAACCTGCT GGCCAGAGCA GCGCCCCCAG CCCAGCCATG 
GAGACCCGAG GAGGCCGCGG AACCCCACAG CTGGCAGAAG GAGCCACAGC CAGGGCCATC 
AGCGACCTGG CGGTGCGCAG GGCCCGCCAC CGGCTGCTCT CTGGGGACTC CATAGAAAAG 
CGCACCGCTC GCCCCATCAA CAAAGTGATC AAGTCCGCCT CAGCCACAGC CCTGTCCCTT 
CTCATTCCTG CTGAGCACCA CTCCTGCTCT CCACTGGCCA GCCCCATGTC CCCACATTCC 
CAGTCATCCA ACCCATCATC CAGGGACTCT TCTCCAAGCA GGGACTTCCT GCCAGCCCTC 
AGCAGCTCAC GGCCCCCCAT TGTCATCCAC CGAGCCGGCA AGAAGTATGG CTTCACCCTA 
CGAGCCATCC GTGTCTACAT GGGTGACTCA GATGTCTACA CCGTGCACCA CATGGTGTGG 
CACGTGGAGG ACGGAGGTCC AGCCAGTGAG GCAGGGCTGC GTCAGGGGGA CCTCATCACG 
CACGTCAACG GGGAGCCTGT GCACGGGCTG GTGCACACGG AGGTGGTGGA GCTGATCCTG 
AAGAGTGGAA ACAAGGTGTC TATTTCAACA ACTCCCCTGG AGAACACCTC CATTAAGGTG 
GGGCCCGCTC GGAAGGGCAG CTACAAGGCC AAGATGGCCC GAAGGAGCAA GCGGAGCCGG 
GGCAAGGATG GACAAGAAAG CAGAAAAAGG AGCTCCCTGT TCCGGAAGAT CACCAAGCAG 
GCATCCCTGC TCCACACCAG CCGCAGCCTT TCTTCCCTTA ACCGCTCCCT GTCGTCCGGC 
GAGAGTGGGC CAGGCTCGCC CACACACAGC CACAGCCTTT CTCCCCGCTC TCCCACGCAG 
GGCTACCGGG TGACCCCTGA AGCCGTGCAC TCAGTGGGGG GGAATTCATC ACAGAGCAGC 
TCCCCCAGCT CCAGCGTGCC CAGTTCTCCT GCCGGCTCTG GGCACACACG GCCGAGCTCC 
CTCCACGGTC TGGCGCCCAA GCTCCAACGC CAGTACCGCT CTCCACGGCG CAAGTCAGCA 
GGCAGCATCC CGCTGTCACC ACTGGCCCAC ACCCCTTCAC CACCACCAGC GGCTTCGCCT 
CAGCGTTCCC CATCACCTCT GTCTGGCCAC GGGGCCCAGG CCTTCCCTAC CAAGCTCCAC 
TTGTCGCCTC CCCTAGGCCG GCAACTCTCA CGGCCCAAGA GCGCGGAGCC ACCCCGCTCA 
CCACTACTTA AGAGGGTGCA GTCAGCTGAA AAACTGGCAG CTGCACTGGC TGCTTCTGAG 
AAGAAGCTCG CCACTTCTCG AAAACAGAGC CTTGACCTGC CCCACCCCGA GCTAAAGAAG 
GAATTGCCGC CCAGGGAAGT CAGCTCTCTG GAGGTAGTTG GGACCAGGAG TGTGCTGTCT 
GGAAAGGGGG CGCTGCCAGG GAAAGGGGTG TTGCAGGCTG CTCCCTCACG GGCCTTAGGG 
ACCCTCCGCC AGGACAGGGC TGAACGGCGA GAGTCGCTGC AGAAGCAGGA AGCCATCCGG 
GAGGTTGACT CTTCAGAAGA TGATACTGAT GAGGGGCCTG AGAACAGCCA AGGTGTTCAG 
GAGCCAAGCT TGGTACCCCA CCGAGAAGGG GGCCAGGATC CACCCCTCAG AGGAGCAGGA 
GAGGGTGAGG AAGAGGATGC CTTCTTGCCC AGGGATCCCA GGGGACAGGG CCCAGTGGTC 
ACAGGTCTAC TGACAGGGGT CACACTGGGA TCTCCCAGAA TGGAAGGCCC TGGTGTCCCC 
CAGAAGAAGC TTGGAATCCT ACAGGCTTTT GAAGAGGGTT CCAGCTCCTC GTCCTCAGCC 
CCCAACCTGG GGAAGGCTGG CCCCACAGAC CCCATCCCCG CTGAAGGCTG CTGGAAGGCC 
CAGCACCTCC ACACCCAGGC ACTGACAGCA CTTTGTCCGA GCTCATCAGG ACGCATCCCT 
GCCAGTTGCT CCACTGCCTC CACCCCTGGA GAGCTGGGCC CATGGTCCTG GAAATTCCTC 
TTCAAGGGCC CTGACAGGGC ATACCCAACC GGAAAGGCAG CAATGGAAGA TGGGCCAGCC 
AGCTCCCAAG ATTTGGAAAC TACAACTCCA GGCCACCCTG CAAACCTGTC CCCCAGACAG 
GAGGGGAAGT CATGGCCACC CAGTGCCCCT GGGCTGGCCC ATCCACCTTG TGAGGTTCCC 
AGCCAGAGCT GGCTATGGGA GTCTGAGTGT GCACAGAGAG AGAAAGAGGA GCCAGCGCTG 
GGCATCACCA AAGTGCCTGA TGCCTCAGGT GACAGGAGGC AGGACGTTCC ATGCAAGAGC 
TGCCCCTTCA CCCCAGAGCC TGGGCCCAGC CTGCTCCAGA AGGGCCAAGA CCCAGGGGGC 
CCTCAGGAGC ATCAGGACTT GGCATTGTTA CCTGATGAGC TTTTAAAGCA AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204940.1
CDS	268..5424
Translation	MMKRRRERLGAPCLRIQISALCRGSEVNRHVLSPTSAPAWSLSKVPFSAECTLATSPLAFFLNTHAHSSPGSPCSSRSLQWSCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_005204883.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204883.3

ORF Insert Sequence:

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG 
TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT 
TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG 
TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA 
CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC 
CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGCCATGGC 
CACAGAGCAG ACAGGACTGA TGGACGGCGC TGGTCTTTGG CCTCATTGCC TTCTTCAGGC 
TATGGAACCA ACACCCCTAG TTCTACCGTG TCATCGTCGT GCTCCTCTCA GGAGAAGCTG 
CACCAGTTGC CTTTCCAGCC CACGGCCGAT GAGCTGCACT TCTTGACGAA GCATTTCAGC 
ACAGACAGTG TGCCTGATGA GGAGGGGCGG CAGTCCCCCG CCCTGCGGCC CCGCTCCCGC 
AGCCTCAGTC CTGGACGGTC ACCAGTTTCC TTTGACAGTG AAATAATAAT GATGAATCAT 
GTATACAAGG AAAGATTCCC CAAGGCCACT GCACAAATGG AAGAGCGTCT GGCCGAGTTC 
ATCTCCTCCA ATACTCCAGA CAACGTGTTG CCCTTGGCTG ATGGAACCTT GAGCTTTATT 
CATCATCAGG TGATAGAGAT GGCCCGAGAC TGCCTGGACA AATCCCGGAG TGGCCTCATT 
ACCTCACACT ACTTCTACGA ACTTCAAGAG AATTTAGAGA AGCTTCTGCA AGATGCTCAT 
GAGCGCTCAG AGAGTTCAGA AGTGGCTTTT GTGATGCAGC TGGTGAAAAA GCTGATGATT 
GTCATTGCCC GCCCAGCTCG TCTCCTGGAA TGCCTGGAGT TTGACCCTGA AGAGTTCTAC 
CACCTGTTAG AAGCAGCTGA GGGCCACGCC AAAGAGGGAC AAGGGATTAA ATGTGACATT 
CCCCGCTACA TCGTTAGCCA ACTGGGCCTC ACACGGGATC CCCTAGAGGA AATGGCCCAG 
TTGAGCAGCT ATGACAGTCC TGATACTCCG GAGACAGATG ATTCAGTCGA GGGCCGTGGG 
GCATCTCTGA CATCGAAAAA GACACCCTCT GAGGAGGACT TCGAAACCAT TAAGCTCATC 
AGCAATGGCG CCTATGGGGC CGTGTTCCTG GTGCGGCACA AGTCCACCCG GCAGCGCTTT 
GCCATGAAGA AGATCAACAA GCAGAACCTG ATCCTGCGGA ACCAGATCCA GCAGGCCTTC 
GTGGAGCGGG ACATCCTGAC TTTTGCTGAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC 
TTTGAAACCA AGCGCCACCT GTGCATGGTG ATGGAGTATG TGGAAGGAGG AGACTGTGCC 
ACGCTGCTGA AGAACATCGG GGCCCTGCCT GTGGACATGG TGCGCCTGTA CTTTGCAGAA 
ACTGTGCTAG CCCTGGAGTA CTTACACAAT TACGGCATCG TGCACCGTGA CCTCAAGCCT 
GACAACCTGC TGATTACATC CATGGGTCAC ATCAAGCTCA CTGATTTTGG CCTTTCCAAA 
ATTGGCCTTA TGAGTCTGAC CACAAACTTG TACGAGGGCC ACATTGAAAA GGATGCACGG 
GAGTTCCTGG ACAAGCAGGT ATGCGGGACC CCGGAGTACA TTGCACCTGA GGTGATCCTG 
CGGCAGGGCT ACGGGAAGCC AGTGGACTGG TGGGCTATGG GCATCATCCT GTACGAGTTC 
CTGGTGGGCT GCGTCCCTTT CTTTGGAGAC ACTCCGGAGG AGCTCTTTGG GCAAGTGATC 
AGTGATGAGA TTGTGTGGCC TGAGGGGGAC GATGCACTGC CGCCGGACGC CCAAGATCTC 
ACCTCAAAAC TGCTCCACCA GAACCCATTG GAGAGACTGG GCACAGGCAG TGCCTATGAG 
GTGAAGCAGC ACCCATTCTT CACTGGTCTG GACTGGACGG GACTTCTTCG CCAGAAGGCT 
GAATTTATTC CTCAGCTGGA GTCAGAGGAT GATACCAGCT ACTTTGACAC CCGCTCAGAG 
CGATACCACC ACGTGGACTC GGAGGATGAG GAGGAAGTGA GTGAGGATGG CTGCCTTGAG 
ATTCGCCAGT TCTCGTCCTG TTCTCCAAGG TTCAGCAAGG TGTACAGCAG CATGGAGCGG 
CTCTCGCTGC TCGAGGAGCG CCGGACGCCA CCCCCCACCA AGCGCAGCCT GAGTGAGGAA 
AAGGAGGACC GCACGGATGG CCTGGCGGGG CTGAGGGGCC GAGAGCGAAG CTGGGTGATC 
GGCTCCCCTG AGATATTACG GAAGCGGCTG TCGGTGTCCG AGTCGTCCCA CACGGAAAGC 
GATTCCAGCC CTCCACTCAC CGTGCGGCGC CACTGCTCGG GCCTCCTGGA CGTGCCTCGC 
TTCCCTGAGA GCACTGAGGA GGCTGTTGGC GCTCCCAGGA GGCAGCAGCA GGAGGGTGCA 
TGGCTTCTGA CCCCGTCTGG AGAGGGGGCG TCTGGGCCTG CCCTGGAACG GCCGGCCGAG 
CGGCGGCTGA AGCTGAATGA GGAACCTGCT GGCCAGAGCA GCGCCCCCAG CCCAGCCATG 
GAGACCCGAG GAGGCCGCGG AACCCCACAG CTGGCAGAAG GAGCCACAGC CAGGGCCATC 
AGCGACCTGG CGGTGCGCAG GGCCCGCCAC CGGCTGCTCT CTGGGGACTC CATAGAAAAG 
CGCACCGCTC GCCCCATCAA CAAAGTGATC AAGTCCGCCT CAGCCACAGC CCTGTCCCTT 
CTCATTCCTG CTGAGCACCA CTCCTGCTCT CCACTGGCCA GCCCCATGTC CCCACATTCC 
CAGTCATCCA ACCCATCATC CAGGGACTCT TCTCCAAGCA GGGACTTCCT GCCAGCCCTC 
AGCAGCTCAC GGCCCCCCAT TGTCATCCAC CGAGCCGGCA AGAAGTATGG CTTCACCCTA 
CGAGCCATCC GTGTCTACAT GGGTGACTCA GATGTCTACA CCGTGCACCA CATGGTGTGG 
CACGTGGAGG ACGGAGGTCC AGCCAGTGAG GCAGGGCTGC GTCAGGGGGA CCTCATCACG 
CACGTCAACG GGGAGCCTGT GCACGGGCTG GTGCACACGG AGGTGGTGGA GCTGATCCTG 
AAGAGTGGAA ACAAGGTGTC TATTTCAACA ACTCCCCTGG AGAACACCTC CATTAAGGTG 
GGGCCCGCTC GGAAGGGCAG CTACAAGGCC AAGATGGCCC GAAGGAGCAA GCGGAGCCGG 
GGCAAGGATG GACAAGAAAG CAGAAAAAGG AGCTCCCTGT TCCGGAAGAT CACCAAGCAG 
GCATCCCTGC TCCACACCAG CCGCAGCCTT TCTTCCCTTA ACCGCTCCCT GTCGTCCGGC 
GAGAGTGGGC CAGGCTCGCC CACACACAGC CACAGCCTTT CTCCCCGCTC TCCCACGCAG 
GGCTACCGGG TGACCCCTGA AGCCGTGCAC TCAGTGGGGG GGAATTCATC ACAGAGCAGC 
TCCCCCAGCT CCAGCGTGCC CAGTTCTCCT GCCGGCTCTG GGCACACACG GCCGAGCTCC 
CTCCACGGTC TGGCGCCCAA GCTCCAACGC CAGTACCGCT CTCCACGGCG CAAGTCAGCA 
GGCAGCATCC CGCTGTCACC ACTGGCCCAC ACCCCTTCAC CACCACCAGC GGCTTCGCCT 
CAGCGTTCCC CATCACCTCT GTCTGGCCAC GGGGCCCAGG CCTTCCCTAC CAAGCTCCAC 
TTGTCGCCTC CCCTAGGCCG GCAACTCTCA CGGCCCAAGA GCGCGGAGCC ACCCCGCTCA 
CCACTACTTA AGAGGGTGCA GTCAGCTGAA AAACTGGCAG CTGCACTGGC TGCTTCTGAG 
AAGAAGCTCG CCACTTCTCG AAAACAGAGC CTTGACCTGC CCCACCCCGA GCTAAAGAAG 
GAATTGCCGC CCAGGGAAGT CAGCTCTCTG GAGGTAGTTG GGACCAGGAG TGTGCTGTCT 
GGAAAGGGGG CGCTGCCAGG GAAAGGGGTG TTGCAGGCTG CTCCCTCACG GGCCTTAGGG 
ACCCTCCGCC AGGACAGGGC TGAACGGCGA GAGTCGCTGC AGAAGCAGGA AGCCATCCGG 
GAGGTTGACT CTTCAGAAGA TGATACTGAT GAGGGGCCTG AGAACAGCCA AGGTGTTCAG 
GAGCCAAGCT TGGTACCCCA CCGAGAAGGG GGCCAGGATC CACCCCTCAG AGGAGCAGGA 
GAGGGTGAGG AAGAGGATGC CTTCTTGCCC AGGGATCCCA GGGGACAGGG CCCAGTGGTC 
ACAGGTCTAC TGACAGGGGT CACACTGGGA TCTCCCAGAA TGGAAGGCCC TGGTGTCCCC 
CAGAAGAAGC TTGGAATCCT ACAGGCTTTT GAAGAGGGTT CCAGCTCCTC GTCCTCAGCC 
CCCAACCTGG GGAAGGCTGG CCCCACAGAC CCCATCCCCG CTGAAGGCTG CTGGAAGGCC 
CAGCACCTCC ACACCCAGGC ACTGACAGCA CTTTGTCCGA GCTCATCAGG ACGCATCCCT 
GCCAGTTGCT CCACTGCCTC CACCCCTGGA GAGCTGGGCC CATGGTCCTG GAAATTCCTC 
TTCAAGGGCC CTGACAGGGC ATACCCAACC GGAAAGGCAG CAATGGAAGA TGGGCCAGCC 
AGCTCCCAAG ATTTGGAAAC TACAACTCCA GGCCACCCTG CAAACCTGTC CCCCAGACAG 
GAGGGGAAGT CATGGCCACC CAGTGCCCCT GGGCTGGCCC ATCCACCTTG TGAGGTTCCC 
AGCCAGAGCT GGCTATGGGA GTCTGAGTGT GCACAGAGAG AGAAAGAGGA GCCAGCGCTG 
GGCATCACCA AAGTGCCTGA TGCCTCAGGT GACAGGAGGC AGGACGTTCC ATGCAAGAGC 
TGCCCCTTCA CCCCAGAGCC TGGGCCCAGC CTGCTCCAGA AGGGCCAAGA CCCAGGGGGC 
CCTCAGGAGC ATCAGGACTT GGCATTGTTA CCTGATGAGC TTTTAAAGCA AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_015462788.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318274.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA.

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CloneID	OBa18162
Clone ID Related Accession (Same CDS sequence)	XM_005204883.3 , XM_015462788.1
Accession Version	XM_015462788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG 
TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT 
TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG 
TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA 
CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC 
CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGCCATGGC 
CACAGAGCAG ACAGGACTGA TGGACGGCGC TGGTCTTTGG CCTCATTGCC TTCTTCAGGC 
TATGGAACCA ACACCCCTAG TTCTACCGTG TCATCGTCGT GCTCCTCTCA GGAGAAGCTG 
CACCAGTTGC CTTTCCAGCC CACGGCCGAT GAGCTGCACT TCTTGACGAA GCATTTCAGC 
ACAGACAGTG TGCCTGATGA GGAGGGGCGG CAGTCCCCCG CCCTGCGGCC CCGCTCCCGC 
AGCCTCAGTC CTGGACGGTC ACCAGTTTCC TTTGACAGTG AAATAATAAT GATGAATCAT 
GTATACAAGG AAAGATTCCC CAAGGCCACT GCACAAATGG AAGAGCGTCT GGCCGAGTTC 
ATCTCCTCCA ATACTCCAGA CAACGTGTTG CCCTTGGCTG ATGGAACCTT GAGCTTTATT 
CATCATCAGG TGATAGAGAT GGCCCGAGAC TGCCTGGACA AATCCCGGAG TGGCCTCATT 
ACCTCACACT ACTTCTACGA ACTTCAAGAG AATTTAGAGA AGCTTCTGCA AGATGCTCAT 
GAGCGCTCAG AGAGTTCAGA AGTGGCTTTT GTGATGCAGC TGGTGAAAAA GCTGATGATT 
GTCATTGCCC GCCCAGCTCG TCTCCTGGAA TGCCTGGAGT TTGACCCTGA AGAGTTCTAC 
CACCTGTTAG AAGCAGCTGA GGGCCACGCC AAAGAGGGAC AAGGGATTAA ATGTGACATT 
CCCCGCTACA TCGTTAGCCA ACTGGGCCTC ACACGGGATC CCCTAGAGGA AATGGCCCAG 
TTGAGCAGCT ATGACAGTCC TGATACTCCG GAGACAGATG ATTCAGTCGA GGGCCGTGGG 
GCATCTCTGA CATCGAAAAA GACACCCTCT GAGGAGGACT TCGAAACCAT TAAGCTCATC 
AGCAATGGCG CCTATGGGGC CGTGTTCCTG GTGCGGCACA AGTCCACCCG GCAGCGCTTT 
GCCATGAAGA AGATCAACAA GCAGAACCTG ATCCTGCGGA ACCAGATCCA GCAGGCCTTC 
GTGGAGCGGG ACATCCTGAC TTTTGCTGAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC 
TTTGAAACCA AGCGCCACCT GTGCATGGTG ATGGAGTATG TGGAAGGAGG AGACTGTGCC 
ACGCTGCTGA AGAACATCGG GGCCCTGCCT GTGGACATGG TGCGCCTGTA CTTTGCAGAA 
ACTGTGCTAG CCCTGGAGTA CTTACACAAT TACGGCATCG TGCACCGTGA CCTCAAGCCT 
GACAACCTGC TGATTACATC CATGGGTCAC ATCAAGCTCA CTGATTTTGG CCTTTCCAAA 
ATTGGCCTTA TGAGTCTGAC CACAAACTTG TACGAGGGCC ACATTGAAAA GGATGCACGG 
GAGTTCCTGG ACAAGCAGGT ATGCGGGACC CCGGAGTACA TTGCACCTGA GGTGATCCTG 
CGGCAGGGCT ACGGGAAGCC AGTGGACTGG TGGGCTATGG GCATCATCCT GTACGAGTTC 
CTGGTGGGCT GCGTCCCTTT CTTTGGAGAC ACTCCGGAGG AGCTCTTTGG GCAAGTGATC 
AGTGATGAGA TTGTGTGGCC TGAGGGGGAC GATGCACTGC CGCCGGACGC CCAAGATCTC 
ACCTCAAAAC TGCTCCACCA GAACCCATTG GAGAGACTGG GCACAGGCAG TGCCTATGAG 
GTGAAGCAGC ACCCATTCTT CACTGGTCTG GACTGGACGG GACTTCTTCG CCAGAAGGCT 
GAATTTATTC CTCAGCTGGA GTCAGAGGAT GATACCAGCT ACTTTGACAC CCGCTCAGAG 
CGATACCACC ACGTGGACTC GGAGGATGAG GAGGAAGTGA GTGAGGATGG CTGCCTTGAG 
ATTCGCCAGT TCTCGTCCTG TTCTCCAAGG TTCAGCAAGG TGTACAGCAG CATGGAGCGG 
CTCTCGCTGC TCGAGGAGCG CCGGACGCCA CCCCCCACCA AGCGCAGCCT GAGTGAGGAA 
AAGGAGGACC GCACGGATGG CCTGGCGGGG CTGAGGGGCC GAGAGCGAAG CTGGGTGATC 
GGCTCCCCTG AGATATTACG GAAGCGGCTG TCGGTGTCCG AGTCGTCCCA CACGGAAAGC 
GATTCCAGCC CTCCACTCAC CGTGCGGCGC CACTGCTCGG GCCTCCTGGA CGTGCCTCGC 
TTCCCTGAGA GCACTGAGGA GGCTGTTGGC GCTCCCAGGA GGCAGCAGCA GGAGGGTGCA 
TGGCTTCTGA CCCCGTCTGG AGAGGGGGCG TCTGGGCCTG CCCTGGAACG GCCGGCCGAG 
CGGCGGCTGA AGCTGAATGA GGAACCTGCT GGCCAGAGCA GCGCCCCCAG CCCAGCCATG 
GAGACCCGAG GAGGCCGCGG AACCCCACAG CTGGCAGAAG GAGCCACAGC CAGGGCCATC 
AGCGACCTGG CGGTGCGCAG GGCCCGCCAC CGGCTGCTCT CTGGGGACTC CATAGAAAAG 
CGCACCGCTC GCCCCATCAA CAAAGTGATC AAGTCCGCCT CAGCCACAGC CCTGTCCCTT 
CTCATTCCTG CTGAGCACCA CTCCTGCTCT CCACTGGCCA GCCCCATGTC CCCACATTCC 
CAGTCATCCA ACCCATCATC CAGGGACTCT TCTCCAAGCA GGGACTTCCT GCCAGCCCTC 
AGCAGCTCAC GGCCCCCCAT TGTCATCCAC CGAGCCGGCA AGAAGTATGG CTTCACCCTA 
CGAGCCATCC GTGTCTACAT GGGTGACTCA GATGTCTACA CCGTGCACCA CATGGTGTGG 
CACGTGGAGG ACGGAGGTCC AGCCAGTGAG GCAGGGCTGC GTCAGGGGGA CCTCATCACG 
CACGTCAACG GGGAGCCTGT GCACGGGCTG GTGCACACGG AGGTGGTGGA GCTGATCCTG 
AAGAGTGGAA ACAAGGTGTC TATTTCAACA ACTCCCCTGG AGAACACCTC CATTAAGGTG 
GGGCCCGCTC GGAAGGGCAG CTACAAGGCC AAGATGGCCC GAAGGAGCAA GCGGAGCCGG 
GGCAAGGATG GACAAGAAAG CAGAAAAAGG AGCTCCCTGT TCCGGAAGAT CACCAAGCAG 
GCATCCCTGC TCCACACCAG CCGCAGCCTT TCTTCCCTTA ACCGCTCCCT GTCGTCCGGC 
GAGAGTGGGC CAGGCTCGCC CACACACAGC CACAGCCTTT CTCCCCGCTC TCCCACGCAG 
GGCTACCGGG TGACCCCTGA AGCCGTGCAC TCAGTGGGGG GGAATTCATC ACAGAGCAGC 
TCCCCCAGCT CCAGCGTGCC CAGTTCTCCT GCCGGCTCTG GGCACACACG GCCGAGCTCC 
CTCCACGGTC TGGCGCCCAA GCTCCAACGC CAGTACCGCT CTCCACGGCG CAAGTCAGCA 
GGCAGCATCC CGCTGTCACC ACTGGCCCAC ACCCCTTCAC CACCACCAGC GGCTTCGCCT 
CAGCGTTCCC CATCACCTCT GTCTGGCCAC GGGGCCCAGG CCTTCCCTAC CAAGCTCCAC 
TTGTCGCCTC CCCTAGGCCG GCAACTCTCA CGGCCCAAGA GCGCGGAGCC ACCCCGCTCA 
CCACTACTTA AGAGGGTGCA GTCAGCTGAA AAACTGGCAG CTGCACTGGC TGCTTCTGAG 
AAGAAGCTCG CCACTTCTCG AAAACAGAGC CTTGACCTGC CCCACCCCGA GCTAAAGAAG 
GAATTGCCGC CCAGGGAAGT CAGCTCTCTG GAGGTAGTTG GGACCAGGAG TGTGCTGTCT 
GGAAAGGGGG CGCTGCCAGG GAAAGGGGTG TTGCAGGCTG CTCCCTCACG GGCCTTAGGG 
ACCCTCCGCC AGGACAGGGC TGAACGGCGA GAGTCGCTGC AGAAGCAGGA AGCCATCCGG 
GAGGTTGACT CTTCAGAAGA TGATACTGAT GAGGGGCCTG AGAACAGCCA AGGTGTTCAG 
GAGCCAAGCT TGGTACCCCA CCGAGAAGGG GGCCAGGATC CACCCCTCAG AGGAGCAGGA 
GAGGGTGAGG AAGAGGATGC CTTCTTGCCC AGGGATCCCA GGGGACAGGG CCCAGTGGTC 
ACAGGTCTAC TGACAGGGGT CACACTGGGA TCTCCCAGAA TGGAAGGCCC TGGTGTCCCC 
CAGAAGAAGC TTGGAATCCT ACAGGCTTTT GAAGAGGGTT CCAGCTCCTC GTCCTCAGCC 
CCCAACCTGG GGAAGGCTGG CCCCACAGAC CCCATCCCCG CTGAAGGCTG CTGGAAGGCC 
CAGCACCTCC ACACCCAGGC ACTGACAGCA CTTTGTCCGA GCTCATCAGG ACGCATCCCT 
GCCAGTTGCT CCACTGCCTC CACCCCTGGA GAGCTGGGCC CATGGTCCTG GAAATTCCTC 
TTCAAGGGCC CTGACAGGGC ATACCCAACC GGAAAGGCAG CAATGGAAGA TGGGCCAGCC 
AGCTCCCAAG ATTTGGAAAC TACAACTCCA GGCCACCCTG CAAACCTGTC CCCCAGACAG 
GAGGGGAAGT CATGGCCACC CAGTGCCCCT GGGCTGGCCC ATCCACCTTG TGAGGTTCCC 
AGCCAGAGCT GGCTATGGGA GTCTGAGTGT GCACAGAGAG AGAAAGAGGA GCCAGCGCTG 
GGCATCACCA AAGTGCCTGA TGCCTCAGGT GACAGGAGGC AGGACGTTCC ATGCAAGAGC 
TGCCCCTTCA CCCCAGAGCC TGGGCCCAGC CTGCTCCAGA AGGGCCAAGA CCCAGGGGGC 
CCTCAGGAGC ATCAGGACTT GGCATTGTTA CCTGATGAGC TTTTAAAGCA AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318274.1
CDS	268..5424
Translation	MMKRRRERLGAPCLRIQISALCRGSEVNRHVLSPTSAPAWSLSKVPFSAECTLATSPLAFFLNTHAHSSPGSPCSSRSLQWSCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_015462788.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462788.1

ORF Insert Sequence:

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG 
TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT 
TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG 
TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA 
CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC 
CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGCCATGGC 
CACAGAGCAG ACAGGACTGA TGGACGGCGC TGGTCTTTGG CCTCATTGCC TTCTTCAGGC 
TATGGAACCA ACACCCCTAG TTCTACCGTG TCATCGTCGT GCTCCTCTCA GGAGAAGCTG 
CACCAGTTGC CTTTCCAGCC CACGGCCGAT GAGCTGCACT TCTTGACGAA GCATTTCAGC 
ACAGACAGTG TGCCTGATGA GGAGGGGCGG CAGTCCCCCG CCCTGCGGCC CCGCTCCCGC 
AGCCTCAGTC CTGGACGGTC ACCAGTTTCC TTTGACAGTG AAATAATAAT GATGAATCAT 
GTATACAAGG AAAGATTCCC CAAGGCCACT GCACAAATGG AAGAGCGTCT GGCCGAGTTC 
ATCTCCTCCA ATACTCCAGA CAACGTGTTG CCCTTGGCTG ATGGAACCTT GAGCTTTATT 
CATCATCAGG TGATAGAGAT GGCCCGAGAC TGCCTGGACA AATCCCGGAG TGGCCTCATT 
ACCTCACACT ACTTCTACGA ACTTCAAGAG AATTTAGAGA AGCTTCTGCA AGATGCTCAT 
GAGCGCTCAG AGAGTTCAGA AGTGGCTTTT GTGATGCAGC TGGTGAAAAA GCTGATGATT 
GTCATTGCCC GCCCAGCTCG TCTCCTGGAA TGCCTGGAGT TTGACCCTGA AGAGTTCTAC 
CACCTGTTAG AAGCAGCTGA GGGCCACGCC AAAGAGGGAC AAGGGATTAA ATGTGACATT 
CCCCGCTACA TCGTTAGCCA ACTGGGCCTC ACACGGGATC CCCTAGAGGA AATGGCCCAG 
TTGAGCAGCT ATGACAGTCC TGATACTCCG GAGACAGATG ATTCAGTCGA GGGCCGTGGG 
GCATCTCTGA CATCGAAAAA GACACCCTCT GAGGAGGACT TCGAAACCAT TAAGCTCATC 
AGCAATGGCG CCTATGGGGC CGTGTTCCTG GTGCGGCACA AGTCCACCCG GCAGCGCTTT 
GCCATGAAGA AGATCAACAA GCAGAACCTG ATCCTGCGGA ACCAGATCCA GCAGGCCTTC 
GTGGAGCGGG ACATCCTGAC TTTTGCTGAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC 
TTTGAAACCA AGCGCCACCT GTGCATGGTG ATGGAGTATG TGGAAGGAGG AGACTGTGCC 
ACGCTGCTGA AGAACATCGG GGCCCTGCCT GTGGACATGG TGCGCCTGTA CTTTGCAGAA 
ACTGTGCTAG CCCTGGAGTA CTTACACAAT TACGGCATCG TGCACCGTGA CCTCAAGCCT 
GACAACCTGC TGATTACATC CATGGGTCAC ATCAAGCTCA CTGATTTTGG CCTTTCCAAA 
ATTGGCCTTA TGAGTCTGAC CACAAACTTG TACGAGGGCC ACATTGAAAA GGATGCACGG 
GAGTTCCTGG ACAAGCAGGT ATGCGGGACC CCGGAGTACA TTGCACCTGA GGTGATCCTG 
CGGCAGGGCT ACGGGAAGCC AGTGGACTGG TGGGCTATGG GCATCATCCT GTACGAGTTC 
CTGGTGGGCT GCGTCCCTTT CTTTGGAGAC ACTCCGGAGG AGCTCTTTGG GCAAGTGATC 
AGTGATGAGA TTGTGTGGCC TGAGGGGGAC GATGCACTGC CGCCGGACGC CCAAGATCTC 
ACCTCAAAAC TGCTCCACCA GAACCCATTG GAGAGACTGG GCACAGGCAG TGCCTATGAG 
GTGAAGCAGC ACCCATTCTT CACTGGTCTG GACTGGACGG GACTTCTTCG CCAGAAGGCT 
GAATTTATTC CTCAGCTGGA GTCAGAGGAT GATACCAGCT ACTTTGACAC CCGCTCAGAG 
CGATACCACC ACGTGGACTC GGAGGATGAG GAGGAAGTGA GTGAGGATGG CTGCCTTGAG 
ATTCGCCAGT TCTCGTCCTG TTCTCCAAGG TTCAGCAAGG TGTACAGCAG CATGGAGCGG 
CTCTCGCTGC TCGAGGAGCG CCGGACGCCA CCCCCCACCA AGCGCAGCCT GAGTGAGGAA 
AAGGAGGACC GCACGGATGG CCTGGCGGGG CTGAGGGGCC GAGAGCGAAG CTGGGTGATC 
GGCTCCCCTG AGATATTACG GAAGCGGCTG TCGGTGTCCG AGTCGTCCCA CACGGAAAGC 
GATTCCAGCC CTCCACTCAC CGTGCGGCGC CACTGCTCGG GCCTCCTGGA CGTGCCTCGC 
TTCCCTGAGA GCACTGAGGA GGCTGTTGGC GCTCCCAGGA GGCAGCAGCA GGAGGGTGCA 
TGGCTTCTGA CCCCGTCTGG AGAGGGGGCG TCTGGGCCTG CCCTGGAACG GCCGGCCGAG 
CGGCGGCTGA AGCTGAATGA GGAACCTGCT GGCCAGAGCA GCGCCCCCAG CCCAGCCATG 
GAGACCCGAG GAGGCCGCGG AACCCCACAG CTGGCAGAAG GAGCCACAGC CAGGGCCATC 
AGCGACCTGG CGGTGCGCAG GGCCCGCCAC CGGCTGCTCT CTGGGGACTC CATAGAAAAG 
CGCACCGCTC GCCCCATCAA CAAAGTGATC AAGTCCGCCT CAGCCACAGC CCTGTCCCTT 
CTCATTCCTG CTGAGCACCA CTCCTGCTCT CCACTGGCCA GCCCCATGTC CCCACATTCC 
CAGTCATCCA ACCCATCATC CAGGGACTCT TCTCCAAGCA GGGACTTCCT GCCAGCCCTC 
AGCAGCTCAC GGCCCCCCAT TGTCATCCAC CGAGCCGGCA AGAAGTATGG CTTCACCCTA 
CGAGCCATCC GTGTCTACAT GGGTGACTCA GATGTCTACA CCGTGCACCA CATGGTGTGG 
CACGTGGAGG ACGGAGGTCC AGCCAGTGAG GCAGGGCTGC GTCAGGGGGA CCTCATCACG 
CACGTCAACG GGGAGCCTGT GCACGGGCTG GTGCACACGG AGGTGGTGGA GCTGATCCTG 
AAGAGTGGAA ACAAGGTGTC TATTTCAACA ACTCCCCTGG AGAACACCTC CATTAAGGTG 
GGGCCCGCTC GGAAGGGCAG CTACAAGGCC AAGATGGCCC GAAGGAGCAA GCGGAGCCGG 
GGCAAGGATG GACAAGAAAG CAGAAAAAGG AGCTCCCTGT TCCGGAAGAT CACCAAGCAG 
GCATCCCTGC TCCACACCAG CCGCAGCCTT TCTTCCCTTA ACCGCTCCCT GTCGTCCGGC 
GAGAGTGGGC CAGGCTCGCC CACACACAGC CACAGCCTTT CTCCCCGCTC TCCCACGCAG 
GGCTACCGGG TGACCCCTGA AGCCGTGCAC TCAGTGGGGG GGAATTCATC ACAGAGCAGC 
TCCCCCAGCT CCAGCGTGCC CAGTTCTCCT GCCGGCTCTG GGCACACACG GCCGAGCTCC 
CTCCACGGTC TGGCGCCCAA GCTCCAACGC CAGTACCGCT CTCCACGGCG CAAGTCAGCA 
GGCAGCATCC CGCTGTCACC ACTGGCCCAC ACCCCTTCAC CACCACCAGC GGCTTCGCCT 
CAGCGTTCCC CATCACCTCT GTCTGGCCAC GGGGCCCAGG CCTTCCCTAC CAAGCTCCAC 
TTGTCGCCTC CCCTAGGCCG GCAACTCTCA CGGCCCAAGA GCGCGGAGCC ACCCCGCTCA 
CCACTACTTA AGAGGGTGCA GTCAGCTGAA AAACTGGCAG CTGCACTGGC TGCTTCTGAG 
AAGAAGCTCG CCACTTCTCG AAAACAGAGC CTTGACCTGC CCCACCCCGA GCTAAAGAAG 
GAATTGCCGC CCAGGGAAGT CAGCTCTCTG GAGGTAGTTG GGACCAGGAG TGTGCTGTCT 
GGAAAGGGGG CGCTGCCAGG GAAAGGGGTG TTGCAGGCTG CTCCCTCACG GGCCTTAGGG 
ACCCTCCGCC AGGACAGGGC TGAACGGCGA GAGTCGCTGC AGAAGCAGGA AGCCATCCGG 
GAGGTTGACT CTTCAGAAGA TGATACTGAT GAGGGGCCTG AGAACAGCCA AGGTGTTCAG 
GAGCCAAGCT TGGTACCCCA CCGAGAAGGG GGCCAGGATC CACCCCTCAG AGGAGCAGGA 
GAGGGTGAGG AAGAGGATGC CTTCTTGCCC AGGGATCCCA GGGGACAGGG CCCAGTGGTC 
ACAGGTCTAC TGACAGGGGT CACACTGGGA TCTCCCAGAA TGGAAGGCCC TGGTGTCCCC 
CAGAAGAAGC TTGGAATCCT ACAGGCTTTT GAAGAGGGTT CCAGCTCCTC GTCCTCAGCC 
CCCAACCTGG GGAAGGCTGG CCCCACAGAC CCCATCCCCG CTGAAGGCTG CTGGAAGGCC 
CAGCACCTCC ACACCCAGGC ACTGACAGCA CTTTGTCCGA GCTCATCAGG ACGCATCCCT 
GCCAGTTGCT CCACTGCCTC CACCCCTGGA GAGCTGGGCC CATGGTCCTG GAAATTCCTC 
TTCAAGGGCC CTGACAGGGC ATACCCAACC GGAAAGGCAG CAATGGAAGA TGGGCCAGCC 
AGCTCCCAAG ATTTGGAAAC TACAACTCCA GGCCACCCTG CAAACCTGTC CCCCAGACAG 
GAGGGGAAGT CATGGCCACC CAGTGCCCCT GGGCTGGCCC ATCCACCTTG TGAGGTTCCC 
AGCCAGAGCT GGCTATGGGA GTCTGAGTGT GCACAGAGAG AGAAAGAGGA GCCAGCGCTG 
GGCATCACCA AAGTGCCTGA TGCCTCAGGT GACAGGAGGC AGGACGTTCC ATGCAAGAGC 
TGCCCCTTCA CCCCAGAGCC TGGGCCCAGC CTGCTCCAGA AGGGCCAAGA CCCAGGGGGC 
CCTCAGGAGC ATCAGGACTT GGCATTGTTA CCTGATGAGC TTTTAAAGCA AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_015462789.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318275.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA.

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CloneID	OBa18163
Clone ID Related Accession (Same CDS sequence)	XM_015462789.1 , XM_005204884.3
Accession Version	XM_015462789.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG 
TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT 
TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG 
TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA 
CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC 
CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGGACTGAT 
GGACGGCGCT GGTCTTTGGC CTCATTGCCT TCTTCAGGCT ATGGAACCAA CACCCCTAGT 
TCTACCGTGT CATCGTCGTG CTCCTCTCAG GAGAAGCTGC ACCAGTTGCC TTTCCAGCCC 
ACGGCCGATG AGCTGCACTT CTTGACGAAG CATTTCAGCA CAGACAGTGT GCCTGATGAG 
GAGGGGCGGC AGTCCCCCGC CCTGCGGCCC CGCTCCCGCA GCCTCAGTCC TGGACGGTCA 
CCAGTTTCCT TTGACAGTGA AATAATAATG ATGAATCATG TATACAAGGA AAGATTCCCC 
AAGGCCACTG CACAAATGGA AGAGCGTCTG GCCGAGTTCA TCTCCTCCAA TACTCCAGAC 
AACGTGTTGC CCTTGGCTGA TGGAACCTTG AGCTTTATTC ATCATCAGGT GATAGAGATG 
GCCCGAGACT GCCTGGACAA ATCCCGGAGT GGCCTCATTA CCTCACACTA CTTCTACGAA 
CTTCAAGAGA ATTTAGAGAA GCTTCTGCAA GATGCTCATG AGCGCTCAGA GAGTTCAGAA 
GTGGCTTTTG TGATGCAGCT GGTGAAAAAG CTGATGATTG TCATTGCCCG CCCAGCTCGT 
CTCCTGGAAT GCCTGGAGTT TGACCCTGAA GAGTTCTACC ACCTGTTAGA AGCAGCTGAG 
GGCCACGCCA AAGAGGGACA AGGGATTAAA TGTGACATTC CCCGCTACAT CGTTAGCCAA 
CTGGGCCTCA CACGGGATCC CCTAGAGGAA ATGGCCCAGT TGAGCAGCTA TGACAGTCCT 
GATACTCCGG AGACAGATGA TTCAGTCGAG GGCCGTGGGG CATCTCTGAC ATCGAAAAAG 
ACACCCTCTG AGGAGGACTT CGAAACCATT AAGCTCATCA GCAATGGCGC CTATGGGGCC 
GTGTTCCTGG TGCGGCACAA GTCCACCCGG CAGCGCTTTG CCATGAAGAA GATCAACAAG 
CAGAACCTGA TCCTGCGGAA CCAGATCCAG CAGGCCTTCG TGGAGCGGGA CATCCTGACT 
TTTGCTGAGA ACCCCTTTGT GGTCAGCATG TTCTGCTCCT TTGAAACCAA GCGCCACCTG 
TGCATGGTGA TGGAGTATGT GGAAGGAGGA GACTGTGCCA CGCTGCTGAA GAACATCGGG 
GCCCTGCCTG TGGACATGGT GCGCCTGTAC TTTGCAGAAA CTGTGCTAGC CCTGGAGTAC 
TTACACAATT ACGGCATCGT GCACCGTGAC CTCAAGCCTG ACAACCTGCT GATTACATCC 
ATGGGTCACA TCAAGCTCAC TGATTTTGGC CTTTCCAAAA TTGGCCTTAT GAGTCTGACC 
ACAAACTTGT ACGAGGGCCA CATTGAAAAG GATGCACGGG AGTTCCTGGA CAAGCAGGTA 
TGCGGGACCC CGGAGTACAT TGCACCTGAG GTGATCCTGC GGCAGGGCTA CGGGAAGCCA 
GTGGACTGGT GGGCTATGGG CATCATCCTG TACGAGTTCC TGGTGGGCTG CGTCCCTTTC 
TTTGGAGACA CTCCGGAGGA GCTCTTTGGG CAAGTGATCA GTGATGAGAT TGTGTGGCCT 
GAGGGGGACG ATGCACTGCC GCCGGACGCC CAAGATCTCA CCTCAAAACT GCTCCACCAG 
AACCCATTGG AGAGACTGGG CACAGGCAGT GCCTATGAGG TGAAGCAGCA CCCATTCTTC 
ACTGGTCTGG ACTGGACGGG ACTTCTTCGC CAGAAGGCTG AATTTATTCC TCAGCTGGAG 
TCAGAGGATG ATACCAGCTA CTTTGACACC CGCTCAGAGC GATACCACCA CGTGGACTCG 
GAGGATGAGG AGGAAGTGAG TGAGGATGGC TGCCTTGAGA TTCGCCAGTT CTCGTCCTGT 
TCTCCAAGGT TCAGCAAGGT GTACAGCAGC ATGGAGCGGC TCTCGCTGCT CGAGGAGCGC 
CGGACGCCAC CCCCCACCAA GCGCAGCCTG AGTGAGGAAA AGGAGGACCG CACGGATGGC 
CTGGCGGGGC TGAGGGGCCG AGAGCGAAGC TGGGTGATCG GCTCCCCTGA GATATTACGG 
AAGCGGCTGT CGGTGTCCGA GTCGTCCCAC ACGGAAAGCG ATTCCAGCCC TCCACTCACC 
GTGCGGCGCC ACTGCTCGGG CCTCCTGGAC GTGCCTCGCT TCCCTGAGAG CACTGAGGAG 
GCTGTTGGCG CTCCCAGGAG GCAGCAGCAG GAGGGTGCAT GGCTTCTGAC CCCGTCTGGA 
GAGGGGGCGT CTGGGCCTGC CCTGGAACGG CCGGCCGAGC GGCGGCTGAA GCTGAATGAG 
GAACCTGCTG GCCAGAGCAG CGCCCCCAGC CCAGCCATGG AGACCCGAGG AGGCCGCGGA 
ACCCCACAGC TGGCAGAAGG AGCCACAGCC AGGGCCATCA GCGACCTGGC GGTGCGCAGG 
GCCCGCCACC GGCTGCTCTC TGGGGACTCC ATAGAAAAGC GCACCGCTCG CCCCATCAAC 
AAAGTGATCA AGTCCGCCTC AGCCACAGCC CTGTCCCTTC TCATTCCTGC TGAGCACCAC 
TCCTGCTCTC CACTGGCCAG CCCCATGTCC CCACATTCCC AGTCATCCAA CCCATCATCC 
AGGGACTCTT CTCCAAGCAG GGACTTCCTG CCAGCCCTCA GCAGCTCACG GCCCCCCATT 
GTCATCCACC GAGCCGGCAA GAAGTATGGC TTCACCCTAC GAGCCATCCG TGTCTACATG 
GGTGACTCAG ATGTCTACAC CGTGCACCAC ATGGTGTGGC ACGTGGAGGA CGGAGGTCCA 
GCCAGTGAGG CAGGGCTGCG TCAGGGGGAC CTCATCACGC ACGTCAACGG GGAGCCTGTG 
CACGGGCTGG TGCACACGGA GGTGGTGGAG CTGATCCTGA AGAGTGGAAA CAAGGTGTCT 
ATTTCAACAA CTCCCCTGGA GAACACCTCC ATTAAGGTGG GGCCCGCTCG GAAGGGCAGC 
TACAAGGCCA AGATGGCCCG AAGGAGCAAG CGGAGCCGGG GCAAGGATGG ACAAGAAAGC 
AGAAAAAGGA GCTCCCTGTT CCGGAAGATC ACCAAGCAGG CATCCCTGCT CCACACCAGC 
CGCAGCCTTT CTTCCCTTAA CCGCTCCCTG TCGTCCGGCG AGAGTGGGCC AGGCTCGCCC 
ACACACAGCC ACAGCCTTTC TCCCCGCTCT CCCACGCAGG GCTACCGGGT GACCCCTGAA 
GCCGTGCACT CAGTGGGGGG GAATTCATCA CAGAGCAGCT CCCCCAGCTC CAGCGTGCCC 
AGTTCTCCTG CCGGCTCTGG GCACACACGG CCGAGCTCCC TCCACGGTCT GGCGCCCAAG 
CTCCAACGCC AGTACCGCTC TCCACGGCGC AAGTCAGCAG GCAGCATCCC GCTGTCACCA 
CTGGCCCACA CCCCTTCACC ACCACCAGCG GCTTCGCCTC AGCGTTCCCC ATCACCTCTG 
TCTGGCCACG GGGCCCAGGC CTTCCCTACC AAGCTCCACT TGTCGCCTCC CCTAGGCCGG 
CAACTCTCAC GGCCCAAGAG CGCGGAGCCA CCCCGCTCAC CACTACTTAA GAGGGTGCAG 
TCAGCTGAAA AACTGGCAGC TGCACTGGCT GCTTCTGAGA AGAAGCTCGC CACTTCTCGA 
AAACAGAGCC TTGACCTGCC CCACCCCGAG CTAAAGAAGG AATTGCCGCC CAGGGAAGTC 
AGCTCTCTGG AGGTAGTTGG GACCAGGAGT GTGCTGTCTG GAAAGGGGGC GCTGCCAGGG 
AAAGGGGTGT TGCAGGCTGC TCCCTCACGG GCCTTAGGGA CCCTCCGCCA GGACAGGGCT 
GAACGGCGAG AGTCGCTGCA GAAGCAGGAA GCCATCCGGG AGGTTGACTC TTCAGAAGAT 
GATACTGATG AGGGGCCTGA GAACAGCCAA GGTGTTCAGG AGCCAAGCTT GGTACCCCAC 
CGAGAAGGGG GCCAGGATCC ACCCCTCAGA GGAGCAGGAG AGGGTGAGGA AGAGGATGCC 
TTCTTGCCCA GGGATCCCAG GGGACAGGGC CCAGTGGTCA CAGGTCTACT GACAGGGGTC 
ACACTGGGAT CTCCCAGAAT GGAAGGCCCT GGTGTCCCCC AGAAGAAGCT TGGAATCCTA 
CAGGCTTTTG AAGAGGGTTC CAGCTCCTCG TCCTCAGCCC CCAACCTGGG GAAGGCTGGC 
CCCACAGACC CCATCCCCGC TGAAGGCTGC TGGAAGGCCC AGCACCTCCA CACCCAGGCA 
CTGACAGCAC TTTGTCCGAG CTCATCAGGA CGCATCCCTG CCAGTTGCTC CACTGCCTCC 
ACCCCTGGAG AGCTGGGCCC ATGGTCCTGG AAATTCCTCT TCAAGGGCCC TGACAGGGCA 
TACCCAACCG GAAAGGCAGC AATGGAAGAT GGGCCAGCCA GCTCCCAAGA TTTGGAAACT 
ACAACTCCAG GCCACCCTGC AAACCTGTCC CCCAGACAGG AGGGGAAGTC ATGGCCACCC 
AGTGCCCCTG GGCTGGCCCA TCCACCTTGT GAGGTTCCCA GCCAGAGCTG GCTATGGGAG 
TCTGAGTGTG CACAGAGAGA GAAAGAGGAG CCAGCGCTGG GCATCACCAA AGTGCCTGAT 
GCCTCAGGTG ACAGGAGGCA GGACGTTCCA TGCAAGAGCT GCCCCTTCAC CCCAGAGCCT 
GGGCCCAGCC TGCTCCAGAA GGGCCAAGAC CCAGGGGGCC CTCAGGAGCA TCAGGACTTG 
GCATTGTTAC CTGATGAGCT TTTAAAGCAA ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318275.1
CDS	267..5402
Translation	MMKRRRERLGAPCLRIQISALCRGSEVNRHVLSPTSAPAWSLSKVPFSAECTLATSPLAFFLNTHAHSSPGSPCSSRSLQWSCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_015462789.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462789.1

ORF Insert Sequence:

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG 
TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT 
TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG 
TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA 
CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC 
CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGGACTGAT 
GGACGGCGCT GGTCTTTGGC CTCATTGCCT TCTTCAGGCT ATGGAACCAA CACCCCTAGT 
TCTACCGTGT CATCGTCGTG CTCCTCTCAG GAGAAGCTGC ACCAGTTGCC TTTCCAGCCC 
ACGGCCGATG AGCTGCACTT CTTGACGAAG CATTTCAGCA CAGACAGTGT GCCTGATGAG 
GAGGGGCGGC AGTCCCCCGC CCTGCGGCCC CGCTCCCGCA GCCTCAGTCC TGGACGGTCA 
CCAGTTTCCT TTGACAGTGA AATAATAATG ATGAATCATG TATACAAGGA AAGATTCCCC 
AAGGCCACTG CACAAATGGA AGAGCGTCTG GCCGAGTTCA TCTCCTCCAA TACTCCAGAC 
AACGTGTTGC CCTTGGCTGA TGGAACCTTG AGCTTTATTC ATCATCAGGT GATAGAGATG 
GCCCGAGACT GCCTGGACAA ATCCCGGAGT GGCCTCATTA CCTCACACTA CTTCTACGAA 
CTTCAAGAGA ATTTAGAGAA GCTTCTGCAA GATGCTCATG AGCGCTCAGA GAGTTCAGAA 
GTGGCTTTTG TGATGCAGCT GGTGAAAAAG CTGATGATTG TCATTGCCCG CCCAGCTCGT 
CTCCTGGAAT GCCTGGAGTT TGACCCTGAA GAGTTCTACC ACCTGTTAGA AGCAGCTGAG 
GGCCACGCCA AAGAGGGACA AGGGATTAAA TGTGACATTC CCCGCTACAT CGTTAGCCAA 
CTGGGCCTCA CACGGGATCC CCTAGAGGAA ATGGCCCAGT TGAGCAGCTA TGACAGTCCT 
GATACTCCGG AGACAGATGA TTCAGTCGAG GGCCGTGGGG CATCTCTGAC ATCGAAAAAG 
ACACCCTCTG AGGAGGACTT CGAAACCATT AAGCTCATCA GCAATGGCGC CTATGGGGCC 
GTGTTCCTGG TGCGGCACAA GTCCACCCGG CAGCGCTTTG CCATGAAGAA GATCAACAAG 
CAGAACCTGA TCCTGCGGAA CCAGATCCAG CAGGCCTTCG TGGAGCGGGA CATCCTGACT 
TTTGCTGAGA ACCCCTTTGT GGTCAGCATG TTCTGCTCCT TTGAAACCAA GCGCCACCTG 
TGCATGGTGA TGGAGTATGT GGAAGGAGGA GACTGTGCCA CGCTGCTGAA GAACATCGGG 
GCCCTGCCTG TGGACATGGT GCGCCTGTAC TTTGCAGAAA CTGTGCTAGC CCTGGAGTAC 
TTACACAATT ACGGCATCGT GCACCGTGAC CTCAAGCCTG ACAACCTGCT GATTACATCC 
ATGGGTCACA TCAAGCTCAC TGATTTTGGC CTTTCCAAAA TTGGCCTTAT GAGTCTGACC 
ACAAACTTGT ACGAGGGCCA CATTGAAAAG GATGCACGGG AGTTCCTGGA CAAGCAGGTA 
TGCGGGACCC CGGAGTACAT TGCACCTGAG GTGATCCTGC GGCAGGGCTA CGGGAAGCCA 
GTGGACTGGT GGGCTATGGG CATCATCCTG TACGAGTTCC TGGTGGGCTG CGTCCCTTTC 
TTTGGAGACA CTCCGGAGGA GCTCTTTGGG CAAGTGATCA GTGATGAGAT TGTGTGGCCT 
GAGGGGGACG ATGCACTGCC GCCGGACGCC CAAGATCTCA CCTCAAAACT GCTCCACCAG 
AACCCATTGG AGAGACTGGG CACAGGCAGT GCCTATGAGG TGAAGCAGCA CCCATTCTTC 
ACTGGTCTGG ACTGGACGGG ACTTCTTCGC CAGAAGGCTG AATTTATTCC TCAGCTGGAG 
TCAGAGGATG ATACCAGCTA CTTTGACACC CGCTCAGAGC GATACCACCA CGTGGACTCG 
GAGGATGAGG AGGAAGTGAG TGAGGATGGC TGCCTTGAGA TTCGCCAGTT CTCGTCCTGT 
TCTCCAAGGT TCAGCAAGGT GTACAGCAGC ATGGAGCGGC TCTCGCTGCT CGAGGAGCGC 
CGGACGCCAC CCCCCACCAA GCGCAGCCTG AGTGAGGAAA AGGAGGACCG CACGGATGGC 
CTGGCGGGGC TGAGGGGCCG AGAGCGAAGC TGGGTGATCG GCTCCCCTGA GATATTACGG 
AAGCGGCTGT CGGTGTCCGA GTCGTCCCAC ACGGAAAGCG ATTCCAGCCC TCCACTCACC 
GTGCGGCGCC ACTGCTCGGG CCTCCTGGAC GTGCCTCGCT TCCCTGAGAG CACTGAGGAG 
GCTGTTGGCG CTCCCAGGAG GCAGCAGCAG GAGGGTGCAT GGCTTCTGAC CCCGTCTGGA 
GAGGGGGCGT CTGGGCCTGC CCTGGAACGG CCGGCCGAGC GGCGGCTGAA GCTGAATGAG 
GAACCTGCTG GCCAGAGCAG CGCCCCCAGC CCAGCCATGG AGACCCGAGG AGGCCGCGGA 
ACCCCACAGC TGGCAGAAGG AGCCACAGCC AGGGCCATCA GCGACCTGGC GGTGCGCAGG 
GCCCGCCACC GGCTGCTCTC TGGGGACTCC ATAGAAAAGC GCACCGCTCG CCCCATCAAC 
AAAGTGATCA AGTCCGCCTC AGCCACAGCC CTGTCCCTTC TCATTCCTGC TGAGCACCAC 
TCCTGCTCTC CACTGGCCAG CCCCATGTCC CCACATTCCC AGTCATCCAA CCCATCATCC 
AGGGACTCTT CTCCAAGCAG GGACTTCCTG CCAGCCCTCA GCAGCTCACG GCCCCCCATT 
GTCATCCACC GAGCCGGCAA GAAGTATGGC TTCACCCTAC GAGCCATCCG TGTCTACATG 
GGTGACTCAG ATGTCTACAC CGTGCACCAC ATGGTGTGGC ACGTGGAGGA CGGAGGTCCA 
GCCAGTGAGG CAGGGCTGCG TCAGGGGGAC CTCATCACGC ACGTCAACGG GGAGCCTGTG 
CACGGGCTGG TGCACACGGA GGTGGTGGAG CTGATCCTGA AGAGTGGAAA CAAGGTGTCT 
ATTTCAACAA CTCCCCTGGA GAACACCTCC ATTAAGGTGG GGCCCGCTCG GAAGGGCAGC 
TACAAGGCCA AGATGGCCCG AAGGAGCAAG CGGAGCCGGG GCAAGGATGG ACAAGAAAGC 
AGAAAAAGGA GCTCCCTGTT CCGGAAGATC ACCAAGCAGG CATCCCTGCT CCACACCAGC 
CGCAGCCTTT CTTCCCTTAA CCGCTCCCTG TCGTCCGGCG AGAGTGGGCC AGGCTCGCCC 
ACACACAGCC ACAGCCTTTC TCCCCGCTCT CCCACGCAGG GCTACCGGGT GACCCCTGAA 
GCCGTGCACT CAGTGGGGGG GAATTCATCA CAGAGCAGCT CCCCCAGCTC CAGCGTGCCC 
AGTTCTCCTG CCGGCTCTGG GCACACACGG CCGAGCTCCC TCCACGGTCT GGCGCCCAAG 
CTCCAACGCC AGTACCGCTC TCCACGGCGC AAGTCAGCAG GCAGCATCCC GCTGTCACCA 
CTGGCCCACA CCCCTTCACC ACCACCAGCG GCTTCGCCTC AGCGTTCCCC ATCACCTCTG 
TCTGGCCACG GGGCCCAGGC CTTCCCTACC AAGCTCCACT TGTCGCCTCC CCTAGGCCGG 
CAACTCTCAC GGCCCAAGAG CGCGGAGCCA CCCCGCTCAC CACTACTTAA GAGGGTGCAG 
TCAGCTGAAA AACTGGCAGC TGCACTGGCT GCTTCTGAGA AGAAGCTCGC CACTTCTCGA 
AAACAGAGCC TTGACCTGCC CCACCCCGAG CTAAAGAAGG AATTGCCGCC CAGGGAAGTC 
AGCTCTCTGG AGGTAGTTGG GACCAGGAGT GTGCTGTCTG GAAAGGGGGC GCTGCCAGGG 
AAAGGGGTGT TGCAGGCTGC TCCCTCACGG GCCTTAGGGA CCCTCCGCCA GGACAGGGCT 
GAACGGCGAG AGTCGCTGCA GAAGCAGGAA GCCATCCGGG AGGTTGACTC TTCAGAAGAT 
GATACTGATG AGGGGCCTGA GAACAGCCAA GGTGTTCAGG AGCCAAGCTT GGTACCCCAC 
CGAGAAGGGG GCCAGGATCC ACCCCTCAGA GGAGCAGGAG AGGGTGAGGA AGAGGATGCC 
TTCTTGCCCA GGGATCCCAG GGGACAGGGC CCAGTGGTCA CAGGTCTACT GACAGGGGTC 
ACACTGGGAT CTCCCAGAAT GGAAGGCCCT GGTGTCCCCC AGAAGAAGCT TGGAATCCTA 
CAGGCTTTTG AAGAGGGTTC CAGCTCCTCG TCCTCAGCCC CCAACCTGGG GAAGGCTGGC 
CCCACAGACC CCATCCCCGC TGAAGGCTGC TGGAAGGCCC AGCACCTCCA CACCCAGGCA 
CTGACAGCAC TTTGTCCGAG CTCATCAGGA CGCATCCCTG CCAGTTGCTC CACTGCCTCC 
ACCCCTGGAG AGCTGGGCCC ATGGTCCTGG AAATTCCTCT TCAAGGGCCC TGACAGGGCA 
TACCCAACCG GAAAGGCAGC AATGGAAGAT GGGCCAGCCA GCTCCCAAGA TTTGGAAACT 
ACAACTCCAG GCCACCCTGC AAACCTGTCC CCCAGACAGG AGGGGAAGTC ATGGCCACCC 
AGTGCCCCTG GGCTGGCCCA TCCACCTTGT GAGGTTCCCA GCCAGAGCTG GCTATGGGAG 
TCTGAGTGTG CACAGAGAGA GAAAGAGGAG CCAGCGCTGG GCATCACCAA AGTGCCTGAT 
GCCTCAGGTG ACAGGAGGCA GGACGTTCCA TGCAAGAGCT GCCCCTTCAC CCCAGAGCCT 
GGGCCCAGCC TGCTCCAGAA GGGCCAAGAC CCAGGGGGCC CTCAGGAGCA TCAGGACTTG 
GCATTGTTAC CTGATGAGCT TTTAAAGCAA ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_005204884.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204941.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA.

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CloneID	OBa18163
Clone ID Related Accession (Same CDS sequence)	XM_015462789.1 , XM_005204884.3
Accession Version	XM_005204884.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204884.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG 
TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT 
TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG 
TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA 
CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC 
CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGGACTGAT 
GGACGGCGCT GGTCTTTGGC CTCATTGCCT TCTTCAGGCT ATGGAACCAA CACCCCTAGT 
TCTACCGTGT CATCGTCGTG CTCCTCTCAG GAGAAGCTGC ACCAGTTGCC TTTCCAGCCC 
ACGGCCGATG AGCTGCACTT CTTGACGAAG CATTTCAGCA CAGACAGTGT GCCTGATGAG 
GAGGGGCGGC AGTCCCCCGC CCTGCGGCCC CGCTCCCGCA GCCTCAGTCC TGGACGGTCA 
CCAGTTTCCT TTGACAGTGA AATAATAATG ATGAATCATG TATACAAGGA AAGATTCCCC 
AAGGCCACTG CACAAATGGA AGAGCGTCTG GCCGAGTTCA TCTCCTCCAA TACTCCAGAC 
AACGTGTTGC CCTTGGCTGA TGGAACCTTG AGCTTTATTC ATCATCAGGT GATAGAGATG 
GCCCGAGACT GCCTGGACAA ATCCCGGAGT GGCCTCATTA CCTCACACTA CTTCTACGAA 
CTTCAAGAGA ATTTAGAGAA GCTTCTGCAA GATGCTCATG AGCGCTCAGA GAGTTCAGAA 
GTGGCTTTTG TGATGCAGCT GGTGAAAAAG CTGATGATTG TCATTGCCCG CCCAGCTCGT 
CTCCTGGAAT GCCTGGAGTT TGACCCTGAA GAGTTCTACC ACCTGTTAGA AGCAGCTGAG 
GGCCACGCCA AAGAGGGACA AGGGATTAAA TGTGACATTC CCCGCTACAT CGTTAGCCAA 
CTGGGCCTCA CACGGGATCC CCTAGAGGAA ATGGCCCAGT TGAGCAGCTA TGACAGTCCT 
GATACTCCGG AGACAGATGA TTCAGTCGAG GGCCGTGGGG CATCTCTGAC ATCGAAAAAG 
ACACCCTCTG AGGAGGACTT CGAAACCATT AAGCTCATCA GCAATGGCGC CTATGGGGCC 
GTGTTCCTGG TGCGGCACAA GTCCACCCGG CAGCGCTTTG CCATGAAGAA GATCAACAAG 
CAGAACCTGA TCCTGCGGAA CCAGATCCAG CAGGCCTTCG TGGAGCGGGA CATCCTGACT 
TTTGCTGAGA ACCCCTTTGT GGTCAGCATG TTCTGCTCCT TTGAAACCAA GCGCCACCTG 
TGCATGGTGA TGGAGTATGT GGAAGGAGGA GACTGTGCCA CGCTGCTGAA GAACATCGGG 
GCCCTGCCTG TGGACATGGT GCGCCTGTAC TTTGCAGAAA CTGTGCTAGC CCTGGAGTAC 
TTACACAATT ACGGCATCGT GCACCGTGAC CTCAAGCCTG ACAACCTGCT GATTACATCC 
ATGGGTCACA TCAAGCTCAC TGATTTTGGC CTTTCCAAAA TTGGCCTTAT GAGTCTGACC 
ACAAACTTGT ACGAGGGCCA CATTGAAAAG GATGCACGGG AGTTCCTGGA CAAGCAGGTA 
TGCGGGACCC CGGAGTACAT TGCACCTGAG GTGATCCTGC GGCAGGGCTA CGGGAAGCCA 
GTGGACTGGT GGGCTATGGG CATCATCCTG TACGAGTTCC TGGTGGGCTG CGTCCCTTTC 
TTTGGAGACA CTCCGGAGGA GCTCTTTGGG CAAGTGATCA GTGATGAGAT TGTGTGGCCT 
GAGGGGGACG ATGCACTGCC GCCGGACGCC CAAGATCTCA CCTCAAAACT GCTCCACCAG 
AACCCATTGG AGAGACTGGG CACAGGCAGT GCCTATGAGG TGAAGCAGCA CCCATTCTTC 
ACTGGTCTGG ACTGGACGGG ACTTCTTCGC CAGAAGGCTG AATTTATTCC TCAGCTGGAG 
TCAGAGGATG ATACCAGCTA CTTTGACACC CGCTCAGAGC GATACCACCA CGTGGACTCG 
GAGGATGAGG AGGAAGTGAG TGAGGATGGC TGCCTTGAGA TTCGCCAGTT CTCGTCCTGT 
TCTCCAAGGT TCAGCAAGGT GTACAGCAGC ATGGAGCGGC TCTCGCTGCT CGAGGAGCGC 
CGGACGCCAC CCCCCACCAA GCGCAGCCTG AGTGAGGAAA AGGAGGACCG CACGGATGGC 
CTGGCGGGGC TGAGGGGCCG AGAGCGAAGC TGGGTGATCG GCTCCCCTGA GATATTACGG 
AAGCGGCTGT CGGTGTCCGA GTCGTCCCAC ACGGAAAGCG ATTCCAGCCC TCCACTCACC 
GTGCGGCGCC ACTGCTCGGG CCTCCTGGAC GTGCCTCGCT TCCCTGAGAG CACTGAGGAG 
GCTGTTGGCG CTCCCAGGAG GCAGCAGCAG GAGGGTGCAT GGCTTCTGAC CCCGTCTGGA 
GAGGGGGCGT CTGGGCCTGC CCTGGAACGG CCGGCCGAGC GGCGGCTGAA GCTGAATGAG 
GAACCTGCTG GCCAGAGCAG CGCCCCCAGC CCAGCCATGG AGACCCGAGG AGGCCGCGGA 
ACCCCACAGC TGGCAGAAGG AGCCACAGCC AGGGCCATCA GCGACCTGGC GGTGCGCAGG 
GCCCGCCACC GGCTGCTCTC TGGGGACTCC ATAGAAAAGC GCACCGCTCG CCCCATCAAC 
AAAGTGATCA AGTCCGCCTC AGCCACAGCC CTGTCCCTTC TCATTCCTGC TGAGCACCAC 
TCCTGCTCTC CACTGGCCAG CCCCATGTCC CCACATTCCC AGTCATCCAA CCCATCATCC 
AGGGACTCTT CTCCAAGCAG GGACTTCCTG CCAGCCCTCA GCAGCTCACG GCCCCCCATT 
GTCATCCACC GAGCCGGCAA GAAGTATGGC TTCACCCTAC GAGCCATCCG TGTCTACATG 
GGTGACTCAG ATGTCTACAC CGTGCACCAC ATGGTGTGGC ACGTGGAGGA CGGAGGTCCA 
GCCAGTGAGG CAGGGCTGCG TCAGGGGGAC CTCATCACGC ACGTCAACGG GGAGCCTGTG 
CACGGGCTGG TGCACACGGA GGTGGTGGAG CTGATCCTGA AGAGTGGAAA CAAGGTGTCT 
ATTTCAACAA CTCCCCTGGA GAACACCTCC ATTAAGGTGG GGCCCGCTCG GAAGGGCAGC 
TACAAGGCCA AGATGGCCCG AAGGAGCAAG CGGAGCCGGG GCAAGGATGG ACAAGAAAGC 
AGAAAAAGGA GCTCCCTGTT CCGGAAGATC ACCAAGCAGG CATCCCTGCT CCACACCAGC 
CGCAGCCTTT CTTCCCTTAA CCGCTCCCTG TCGTCCGGCG AGAGTGGGCC AGGCTCGCCC 
ACACACAGCC ACAGCCTTTC TCCCCGCTCT CCCACGCAGG GCTACCGGGT GACCCCTGAA 
GCCGTGCACT CAGTGGGGGG GAATTCATCA CAGAGCAGCT CCCCCAGCTC CAGCGTGCCC 
AGTTCTCCTG CCGGCTCTGG GCACACACGG CCGAGCTCCC TCCACGGTCT GGCGCCCAAG 
CTCCAACGCC AGTACCGCTC TCCACGGCGC AAGTCAGCAG GCAGCATCCC GCTGTCACCA 
CTGGCCCACA CCCCTTCACC ACCACCAGCG GCTTCGCCTC AGCGTTCCCC ATCACCTCTG 
TCTGGCCACG GGGCCCAGGC CTTCCCTACC AAGCTCCACT TGTCGCCTCC CCTAGGCCGG 
CAACTCTCAC GGCCCAAGAG CGCGGAGCCA CCCCGCTCAC CACTACTTAA GAGGGTGCAG 
TCAGCTGAAA AACTGGCAGC TGCACTGGCT GCTTCTGAGA AGAAGCTCGC CACTTCTCGA 
AAACAGAGCC TTGACCTGCC CCACCCCGAG CTAAAGAAGG AATTGCCGCC CAGGGAAGTC 
AGCTCTCTGG AGGTAGTTGG GACCAGGAGT GTGCTGTCTG GAAAGGGGGC GCTGCCAGGG 
AAAGGGGTGT TGCAGGCTGC TCCCTCACGG GCCTTAGGGA CCCTCCGCCA GGACAGGGCT 
GAACGGCGAG AGTCGCTGCA GAAGCAGGAA GCCATCCGGG AGGTTGACTC TTCAGAAGAT 
GATACTGATG AGGGGCCTGA GAACAGCCAA GGTGTTCAGG AGCCAAGCTT GGTACCCCAC 
CGAGAAGGGG GCCAGGATCC ACCCCTCAGA GGAGCAGGAG AGGGTGAGGA AGAGGATGCC 
TTCTTGCCCA GGGATCCCAG GGGACAGGGC CCAGTGGTCA CAGGTCTACT GACAGGGGTC 
ACACTGGGAT CTCCCAGAAT GGAAGGCCCT GGTGTCCCCC AGAAGAAGCT TGGAATCCTA 
CAGGCTTTTG AAGAGGGTTC CAGCTCCTCG TCCTCAGCCC CCAACCTGGG GAAGGCTGGC 
CCCACAGACC CCATCCCCGC TGAAGGCTGC TGGAAGGCCC AGCACCTCCA CACCCAGGCA 
CTGACAGCAC TTTGTCCGAG CTCATCAGGA CGCATCCCTG CCAGTTGCTC CACTGCCTCC 
ACCCCTGGAG AGCTGGGCCC ATGGTCCTGG AAATTCCTCT TCAAGGGCCC TGACAGGGCA 
TACCCAACCG GAAAGGCAGC AATGGAAGAT GGGCCAGCCA GCTCCCAAGA TTTGGAAACT 
ACAACTCCAG GCCACCCTGC AAACCTGTCC CCCAGACAGG AGGGGAAGTC ATGGCCACCC 
AGTGCCCCTG GGCTGGCCCA TCCACCTTGT GAGGTTCCCA GCCAGAGCTG GCTATGGGAG 
TCTGAGTGTG CACAGAGAGA GAAAGAGGAG CCAGCGCTGG GCATCACCAA AGTGCCTGAT 
GCCTCAGGTG ACAGGAGGCA GGACGTTCCA TGCAAGAGCT GCCCCTTCAC CCCAGAGCCT 
GGGCCCAGCC TGCTCCAGAA GGGCCAAGAC CCAGGGGGCC CTCAGGAGCA TCAGGACTTG 
GCATTGTTAC CTGATGAGCT TTTAAAGCAA ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204941.1
CDS	267..5402
Translation	MMKRRRERLGAPCLRIQISALCRGSEVNRHVLSPTSAPAWSLSKVPFSAECTLATSPLAFFLNTHAHSSPGSPCSSRSLQWSCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_005204884.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204884.3

ORF Insert Sequence:

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG 
TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT 
TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG 
TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA 
CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC 
CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGGACTGAT 
GGACGGCGCT GGTCTTTGGC CTCATTGCCT TCTTCAGGCT ATGGAACCAA CACCCCTAGT 
TCTACCGTGT CATCGTCGTG CTCCTCTCAG GAGAAGCTGC ACCAGTTGCC TTTCCAGCCC 
ACGGCCGATG AGCTGCACTT CTTGACGAAG CATTTCAGCA CAGACAGTGT GCCTGATGAG 
GAGGGGCGGC AGTCCCCCGC CCTGCGGCCC CGCTCCCGCA GCCTCAGTCC TGGACGGTCA 
CCAGTTTCCT TTGACAGTGA AATAATAATG ATGAATCATG TATACAAGGA AAGATTCCCC 
AAGGCCACTG CACAAATGGA AGAGCGTCTG GCCGAGTTCA TCTCCTCCAA TACTCCAGAC 
AACGTGTTGC CCTTGGCTGA TGGAACCTTG AGCTTTATTC ATCATCAGGT GATAGAGATG 
GCCCGAGACT GCCTGGACAA ATCCCGGAGT GGCCTCATTA CCTCACACTA CTTCTACGAA 
CTTCAAGAGA ATTTAGAGAA GCTTCTGCAA GATGCTCATG AGCGCTCAGA GAGTTCAGAA 
GTGGCTTTTG TGATGCAGCT GGTGAAAAAG CTGATGATTG TCATTGCCCG CCCAGCTCGT 
CTCCTGGAAT GCCTGGAGTT TGACCCTGAA GAGTTCTACC ACCTGTTAGA AGCAGCTGAG 
GGCCACGCCA AAGAGGGACA AGGGATTAAA TGTGACATTC CCCGCTACAT CGTTAGCCAA 
CTGGGCCTCA CACGGGATCC CCTAGAGGAA ATGGCCCAGT TGAGCAGCTA TGACAGTCCT 
GATACTCCGG AGACAGATGA TTCAGTCGAG GGCCGTGGGG CATCTCTGAC ATCGAAAAAG 
ACACCCTCTG AGGAGGACTT CGAAACCATT AAGCTCATCA GCAATGGCGC CTATGGGGCC 
GTGTTCCTGG TGCGGCACAA GTCCACCCGG CAGCGCTTTG CCATGAAGAA GATCAACAAG 
CAGAACCTGA TCCTGCGGAA CCAGATCCAG CAGGCCTTCG TGGAGCGGGA CATCCTGACT 
TTTGCTGAGA ACCCCTTTGT GGTCAGCATG TTCTGCTCCT TTGAAACCAA GCGCCACCTG 
TGCATGGTGA TGGAGTATGT GGAAGGAGGA GACTGTGCCA CGCTGCTGAA GAACATCGGG 
GCCCTGCCTG TGGACATGGT GCGCCTGTAC TTTGCAGAAA CTGTGCTAGC CCTGGAGTAC 
TTACACAATT ACGGCATCGT GCACCGTGAC CTCAAGCCTG ACAACCTGCT GATTACATCC 
ATGGGTCACA TCAAGCTCAC TGATTTTGGC CTTTCCAAAA TTGGCCTTAT GAGTCTGACC 
ACAAACTTGT ACGAGGGCCA CATTGAAAAG GATGCACGGG AGTTCCTGGA CAAGCAGGTA 
TGCGGGACCC CGGAGTACAT TGCACCTGAG GTGATCCTGC GGCAGGGCTA CGGGAAGCCA 
GTGGACTGGT GGGCTATGGG CATCATCCTG TACGAGTTCC TGGTGGGCTG CGTCCCTTTC 
TTTGGAGACA CTCCGGAGGA GCTCTTTGGG CAAGTGATCA GTGATGAGAT TGTGTGGCCT 
GAGGGGGACG ATGCACTGCC GCCGGACGCC CAAGATCTCA CCTCAAAACT GCTCCACCAG 
AACCCATTGG AGAGACTGGG CACAGGCAGT GCCTATGAGG TGAAGCAGCA CCCATTCTTC 
ACTGGTCTGG ACTGGACGGG ACTTCTTCGC CAGAAGGCTG AATTTATTCC TCAGCTGGAG 
TCAGAGGATG ATACCAGCTA CTTTGACACC CGCTCAGAGC GATACCACCA CGTGGACTCG 
GAGGATGAGG AGGAAGTGAG TGAGGATGGC TGCCTTGAGA TTCGCCAGTT CTCGTCCTGT 
TCTCCAAGGT TCAGCAAGGT GTACAGCAGC ATGGAGCGGC TCTCGCTGCT CGAGGAGCGC 
CGGACGCCAC CCCCCACCAA GCGCAGCCTG AGTGAGGAAA AGGAGGACCG CACGGATGGC 
CTGGCGGGGC TGAGGGGCCG AGAGCGAAGC TGGGTGATCG GCTCCCCTGA GATATTACGG 
AAGCGGCTGT CGGTGTCCGA GTCGTCCCAC ACGGAAAGCG ATTCCAGCCC TCCACTCACC 
GTGCGGCGCC ACTGCTCGGG CCTCCTGGAC GTGCCTCGCT TCCCTGAGAG CACTGAGGAG 
GCTGTTGGCG CTCCCAGGAG GCAGCAGCAG GAGGGTGCAT GGCTTCTGAC CCCGTCTGGA 
GAGGGGGCGT CTGGGCCTGC CCTGGAACGG CCGGCCGAGC GGCGGCTGAA GCTGAATGAG 
GAACCTGCTG GCCAGAGCAG CGCCCCCAGC CCAGCCATGG AGACCCGAGG AGGCCGCGGA 
ACCCCACAGC TGGCAGAAGG AGCCACAGCC AGGGCCATCA GCGACCTGGC GGTGCGCAGG 
GCCCGCCACC GGCTGCTCTC TGGGGACTCC ATAGAAAAGC GCACCGCTCG CCCCATCAAC 
AAAGTGATCA AGTCCGCCTC AGCCACAGCC CTGTCCCTTC TCATTCCTGC TGAGCACCAC 
TCCTGCTCTC CACTGGCCAG CCCCATGTCC CCACATTCCC AGTCATCCAA CCCATCATCC 
AGGGACTCTT CTCCAAGCAG GGACTTCCTG CCAGCCCTCA GCAGCTCACG GCCCCCCATT 
GTCATCCACC GAGCCGGCAA GAAGTATGGC TTCACCCTAC GAGCCATCCG TGTCTACATG 
GGTGACTCAG ATGTCTACAC CGTGCACCAC ATGGTGTGGC ACGTGGAGGA CGGAGGTCCA 
GCCAGTGAGG CAGGGCTGCG TCAGGGGGAC CTCATCACGC ACGTCAACGG GGAGCCTGTG 
CACGGGCTGG TGCACACGGA GGTGGTGGAG CTGATCCTGA AGAGTGGAAA CAAGGTGTCT 
ATTTCAACAA CTCCCCTGGA GAACACCTCC ATTAAGGTGG GGCCCGCTCG GAAGGGCAGC 
TACAAGGCCA AGATGGCCCG AAGGAGCAAG CGGAGCCGGG GCAAGGATGG ACAAGAAAGC 
AGAAAAAGGA GCTCCCTGTT CCGGAAGATC ACCAAGCAGG CATCCCTGCT CCACACCAGC 
CGCAGCCTTT CTTCCCTTAA CCGCTCCCTG TCGTCCGGCG AGAGTGGGCC AGGCTCGCCC 
ACACACAGCC ACAGCCTTTC TCCCCGCTCT CCCACGCAGG GCTACCGGGT GACCCCTGAA 
GCCGTGCACT CAGTGGGGGG GAATTCATCA CAGAGCAGCT CCCCCAGCTC CAGCGTGCCC 
AGTTCTCCTG CCGGCTCTGG GCACACACGG CCGAGCTCCC TCCACGGTCT GGCGCCCAAG 
CTCCAACGCC AGTACCGCTC TCCACGGCGC AAGTCAGCAG GCAGCATCCC GCTGTCACCA 
CTGGCCCACA CCCCTTCACC ACCACCAGCG GCTTCGCCTC AGCGTTCCCC ATCACCTCTG 
TCTGGCCACG GGGCCCAGGC CTTCCCTACC AAGCTCCACT TGTCGCCTCC CCTAGGCCGG 
CAACTCTCAC GGCCCAAGAG CGCGGAGCCA CCCCGCTCAC CACTACTTAA GAGGGTGCAG 
TCAGCTGAAA AACTGGCAGC TGCACTGGCT GCTTCTGAGA AGAAGCTCGC CACTTCTCGA 
AAACAGAGCC TTGACCTGCC CCACCCCGAG CTAAAGAAGG AATTGCCGCC CAGGGAAGTC 
AGCTCTCTGG AGGTAGTTGG GACCAGGAGT GTGCTGTCTG GAAAGGGGGC GCTGCCAGGG 
AAAGGGGTGT TGCAGGCTGC TCCCTCACGG GCCTTAGGGA CCCTCCGCCA GGACAGGGCT 
GAACGGCGAG AGTCGCTGCA GAAGCAGGAA GCCATCCGGG AGGTTGACTC TTCAGAAGAT 
GATACTGATG AGGGGCCTGA GAACAGCCAA GGTGTTCAGG AGCCAAGCTT GGTACCCCAC 
CGAGAAGGGG GCCAGGATCC ACCCCTCAGA GGAGCAGGAG AGGGTGAGGA AGAGGATGCC 
TTCTTGCCCA GGGATCCCAG GGGACAGGGC CCAGTGGTCA CAGGTCTACT GACAGGGGTC 
ACACTGGGAT CTCCCAGAAT GGAAGGCCCT GGTGTCCCCC AGAAGAAGCT TGGAATCCTA 
CAGGCTTTTG AAGAGGGTTC CAGCTCCTCG TCCTCAGCCC CCAACCTGGG GAAGGCTGGC 
CCCACAGACC CCATCCCCGC TGAAGGCTGC TGGAAGGCCC AGCACCTCCA CACCCAGGCA 
CTGACAGCAC TTTGTCCGAG CTCATCAGGA CGCATCCCTG CCAGTTGCTC CACTGCCTCC 
ACCCCTGGAG AGCTGGGCCC ATGGTCCTGG AAATTCCTCT TCAAGGGCCC TGACAGGGCA 
TACCCAACCG GAAAGGCAGC AATGGAAGAT GGGCCAGCCA GCTCCCAAGA TTTGGAAACT 
ACAACTCCAG GCCACCCTGC AAACCTGTCC CCCAGACAGG AGGGGAAGTC ATGGCCACCC 
AGTGCCCCTG GGCTGGCCCA TCCACCTTGT GAGGTTCCCA GCCAGAGCTG GCTATGGGAG 
TCTGAGTGTG CACAGAGAGA GAAAGAGGAG CCAGCGCTGG GCATCACCAA AGTGCCTGAT 
GCCTCAGGTG ACAGGAGGCA GGACGTTCCA TGCAAGAGCT GCCCCTTCAC CCCAGAGCCT 
GGGCCCAGCC TGCTCCAGAA GGGCCAAGAC CCAGGGGGCC CTCAGGAGCA TCAGGACTTG 
GCATTGTTAC CTGATGAGCT TTTAAAGCAA ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_005204886.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204943.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa18165
Clone ID Related Accession (Same CDS sequence)	XM_005204886.3 , XM_005204886.4 , XM_015462790.1
Accession Version	XM_005204886.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA CTGTCGGACA 
AGTAACCGCA AGAGTTTGAT TGTGACCTCT AGTACGTCAC CTACACTGCC ACGGCCACAC 
TCACCACTCC ATGGCCACAC AGGTAACAGT CCTTTGGATA GTCCCCGGAA TTTCTCTCCA 
AATGCACCTG CTCACTTTTC CTTTGTTCCT GCCCGTAGCC ATGGCCACAG AGCAGACAGG 
ACTGATGGAC GGCGCTGGTC TTTGGCCTCA TTGCCTTCTT CAGGCTATGG AACCAACACC 
CCTAGTTCTA CCGTGTCATC GTCGTGCTCC TCTCAGGAGA AGCTGCACCA GTTGCCTTTC 
CAGCCCACGG CCGATGAGCT GCACTTCTTG ACGAAGCATT TCAGCACAGA CAGTGTGCCT 
GATGAGGAGG GGCGGCAGTC CCCCGCCCTG CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA 
CGGTCACCAG TTTCCTTTGA CAGTGAAATA ATAATGATGA ATCATGTATA CAAGGAAAGA 
TTCCCCAAGG CCACTGCACA AATGGAAGAG CGTCTGGCCG AGTTCATCTC CTCCAATACT 
CCAGACAACG TGTTGCCCTT GGCTGATGGA ACCTTGAGCT TTATTCATCA TCAGGTGATA 
GAGATGGCCC GAGACTGCCT GGACAAATCC CGGAGTGGCC TCATTACCTC ACACTACTTC 
TACGAACTTC AAGAGAATTT AGAGAAGCTT CTGCAAGATG CTCATGAGCG CTCAGAGAGT 
TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG AAAAAGCTGA TGATTGTCAT TGCCCGCCCA 
GCTCGTCTCC TGGAATGCCT GGAGTTTGAC CCTGAAGAGT TCTACCACCT GTTAGAAGCA 
GCTGAGGGCC ACGCCAAAGA GGGACAAGGG ATTAAATGTG ACATTCCCCG CTACATCGTT 
AGCCAACTGG GCCTCACACG GGATCCCCTA GAGGAAATGG CCCAGTTGAG CAGCTATGAC 
AGTCCTGATA CTCCGGAGAC AGATGATTCA GTCGAGGGCC GTGGGGCATC TCTGACATCG 
AAAAAGACAC CCTCTGAGGA GGACTTCGAA ACCATTAAGC TCATCAGCAA TGGCGCCTAT 
GGGGCCGTGT TCCTGGTGCG GCACAAGTCC ACCCGGCAGC GCTTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTGATCCT GCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGGGACATC 
CTGACTTTTG CTGAGAACCC CTTTGTGGTC AGCATGTTCT GCTCCTTTGA AACCAAGCGC 
CACCTGTGCA TGGTGATGGA GTATGTGGAA GGAGGAGACT GTGCCACGCT GCTGAAGAAC 
ATCGGGGCCC TGCCTGTGGA CATGGTGCGC CTGTACTTTG CAGAAACTGT GCTAGCCCTG 
GAGTACTTAC ACAATTACGG CATCGTGCAC CGTGACCTCA AGCCTGACAA CCTGCTGATT 
ACATCCATGG GTCACATCAA GCTCACTGAT TTTGGCCTTT CCAAAATTGG CCTTATGAGT 
CTGACCACAA ACTTGTACGA GGGCCACATT GAAAAGGATG CACGGGAGTT CCTGGACAAG 
CAGGTATGCG GGACCCCGGA GTACATTGCA CCTGAGGTGA TCCTGCGGCA GGGCTACGGG 
AAGCCAGTGG ACTGGTGGGC TATGGGCATC ATCCTGTACG AGTTCCTGGT GGGCTGCGTC 
CCTTTCTTTG GAGACACTCC GGAGGAGCTC TTTGGGCAAG TGATCAGTGA TGAGATTGTG 
TGGCCTGAGG GGGACGATGC ACTGCCGCCG GACGCCCAAG ATCTCACCTC AAAACTGCTC 
CACCAGAACC CATTGGAGAG ACTGGGCACA GGCAGTGCCT ATGAGGTGAA GCAGCACCCA 
TTCTTCACTG GTCTGGACTG GACGGGACTT CTTCGCCAGA AGGCTGAATT TATTCCTCAG 
CTGGAGTCAG AGGATGATAC CAGCTACTTT GACACCCGCT CAGAGCGATA CCACCACGTG 
GACTCGGAGG ATGAGGAGGA AGTGAGTGAG GATGGCTGCC TTGAGATTCG CCAGTTCTCG 
TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC AGCAGCATGG AGCGGCTCTC GCTGCTCGAG 
GAGCGCCGGA CGCCACCCCC CACCAAGCGC AGCCTGAGTG AGGAAAAGGA GGACCGCACG 
GATGGCCTGG CGGGGCTGAG GGGCCGAGAG CGAAGCTGGG TGATCGGCTC CCCTGAGATA 
TTACGGAAGC GGCTGTCGGT GTCCGAGTCG TCCCACACGG AAAGCGATTC CAGCCCTCCA 
CTCACCGTGC GGCGCCACTG CTCGGGCCTC CTGGACGTGC CTCGCTTCCC TGAGAGCACT 
GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG CAGCAGGAGG GTGCATGGCT TCTGACCCCG 
TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG GAACGGCCGG CCGAGCGGCG GCTGAAGCTG 
AATGAGGAAC CTGCTGGCCA GAGCAGCGCC CCCAGCCCAG CCATGGAGAC CCGAGGAGGC 
CGCGGAACCC CACAGCTGGC AGAAGGAGCC ACAGCCAGGG CCATCAGCGA CCTGGCGGTG 
CGCAGGGCCC GCCACCGGCT GCTCTCTGGG GACTCCATAG AAAAGCGCAC CGCTCGCCCC 
ATCAACAAAG TGATCAAGTC CGCCTCAGCC ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG 
CACCACTCCT GCTCTCCACT GGCCAGCCCC ATGTCCCCAC ATTCCCAGTC ATCCAACCCA 
TCATCCAGGG ACTCTTCTCC AAGCAGGGAC TTCCTGCCAG CCCTCAGCAG CTCACGGCCC 
CCCATTGTCA TCCACCGAGC CGGCAAGAAG TATGGCTTCA CCCTACGAGC CATCCGTGTC 
TACATGGGTG ACTCAGATGT CTACACCGTG CACCACATGG TGTGGCACGT GGAGGACGGA 
GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG GGGGACCTCA TCACGCACGT CAACGGGGAG 
CCTGTGCACG GGCTGGTGCA CACGGAGGTG GTGGAGCTGA TCCTGAAGAG TGGAAACAAG 
GTGTCTATTT CAACAACTCC CCTGGAGAAC ACCTCCATTA AGGTGGGGCC CGCTCGGAAG 
GGCAGCTACA AGGCCAAGAT GGCCCGAAGG AGCAAGCGGA GCCGGGGCAA GGATGGACAA 
GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG AAGATCACCA AGCAGGCATC CCTGCTCCAC 
ACCAGCCGCA GCCTTTCTTC CCTTAACCGC TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC 
TCGCCCACAC ACAGCCACAG CCTTTCTCCC CGCTCTCCCA CGCAGGGCTA CCGGGTGACC 
CCTGAAGCCG TGCACTCAGT GGGGGGGAAT TCATCACAGA GCAGCTCCCC CAGCTCCAGC 
GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC ACACGGCCGA GCTCCCTCCA CGGTCTGGCG 
CCCAAGCTCC AACGCCAGTA CCGCTCTCCA CGGCGCAAGT CAGCAGGCAG CATCCCGCTG 
TCACCACTGG CCCACACCCC TTCACCACCA CCAGCGGCTT CGCCTCAGCG TTCCCCATCA 
CCTCTGTCTG GCCACGGGGC CCAGGCCTTC CCTACCAAGC TCCACTTGTC GCCTCCCCTA 
GGCCGGCAAC TCTCACGGCC CAAGAGCGCG GAGCCACCCC GCTCACCACT ACTTAAGAGG 
GTGCAGTCAG CTGAAAAACT GGCAGCTGCA CTGGCTGCTT CTGAGAAGAA GCTCGCCACT 
TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC CCCGAGCTAA AGAAGGAATT GCCGCCCAGG 
GAAGTCAGCT CTCTGGAGGT AGTTGGGACC AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG 
CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC TCACGGGCCT TAGGGACCCT CCGCCAGGAC 
AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG CAGGAAGCCA TCCGGGAGGT TGACTCTTCA 
GAAGATGATA CTGATGAGGG GCCTGAGAAC AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA 
CCCCACCGAG AAGGGGGCCA GGATCCACCC CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG 
GATGCCTTCT TGCCCAGGGA TCCCAGGGGA CAGGGCCCAG TGGTCACAGG TCTACTGACA 
GGGGTCACAC TGGGATCTCC CAGAATGGAA GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA 
ATCCTACAGG CTTTTGAAGA GGGTTCCAGC TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG 
GCTGGCCCCA CAGACCCCAT CCCCGCTGAA GGCTGCTGGA AGGCCCAGCA CCTCCACACC 
CAGGCACTGA CAGCACTTTG TCCGAGCTCA TCAGGACGCA TCCCTGCCAG TTGCTCCACT 
GCCTCCACCC CTGGAGAGCT GGGCCCATGG TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC 
AGGGCATACC CAACCGGAAA GGCAGCAATG GAAGATGGGC CAGCCAGCTC CCAAGATTTG 
GAAACTACAA CTCCAGGCCA CCCTGCAAAC CTGTCCCCCA GACAGGAGGG GAAGTCATGG 
CCACCCAGTG CCCCTGGGCT GGCCCATCCA CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA 
TGGGAGTCTG AGTGTGCACA GAGAGAGAAA GAGGAGCCAG CGCTGGGCAT CACCAAAGTG 
CCTGATGCCT CAGGTGACAG GAGGCAGGAC GTTCCATGCA AGAGCTGCCC CTTCACCCCA 
GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC CAAGACCCAG GGGGCCCTCA GGAGCATCAG 
GACTTGGCAT TGTTACCTGA TGAGCTTTTA AAGCAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204943.1
CDS	263..5224
Translation	MMKRRRERLGAPCLRIHCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_005204886.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204886.3

ORF Insert Sequence:

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA CTGTCGGACA 
AGTAACCGCA AGAGTTTGAT TGTGACCTCT AGTACGTCAC CTACACTGCC ACGGCCACAC 
TCACCACTCC ATGGCCACAC AGGTAACAGT CCTTTGGATA GTCCCCGGAA TTTCTCTCCA 
AATGCACCTG CTCACTTTTC CTTTGTTCCT GCCCGTAGCC ATGGCCACAG AGCAGACAGG 
ACTGATGGAC GGCGCTGGTC TTTGGCCTCA TTGCCTTCTT CAGGCTATGG AACCAACACC 
CCTAGTTCTA CCGTGTCATC GTCGTGCTCC TCTCAGGAGA AGCTGCACCA GTTGCCTTTC 
CAGCCCACGG CCGATGAGCT GCACTTCTTG ACGAAGCATT TCAGCACAGA CAGTGTGCCT 
GATGAGGAGG GGCGGCAGTC CCCCGCCCTG CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA 
CGGTCACCAG TTTCCTTTGA CAGTGAAATA ATAATGATGA ATCATGTATA CAAGGAAAGA 
TTCCCCAAGG CCACTGCACA AATGGAAGAG CGTCTGGCCG AGTTCATCTC CTCCAATACT 
CCAGACAACG TGTTGCCCTT GGCTGATGGA ACCTTGAGCT TTATTCATCA TCAGGTGATA 
GAGATGGCCC GAGACTGCCT GGACAAATCC CGGAGTGGCC TCATTACCTC ACACTACTTC 
TACGAACTTC AAGAGAATTT AGAGAAGCTT CTGCAAGATG CTCATGAGCG CTCAGAGAGT 
TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG AAAAAGCTGA TGATTGTCAT TGCCCGCCCA 
GCTCGTCTCC TGGAATGCCT GGAGTTTGAC CCTGAAGAGT TCTACCACCT GTTAGAAGCA 
GCTGAGGGCC ACGCCAAAGA GGGACAAGGG ATTAAATGTG ACATTCCCCG CTACATCGTT 
AGCCAACTGG GCCTCACACG GGATCCCCTA GAGGAAATGG CCCAGTTGAG CAGCTATGAC 
AGTCCTGATA CTCCGGAGAC AGATGATTCA GTCGAGGGCC GTGGGGCATC TCTGACATCG 
AAAAAGACAC CCTCTGAGGA GGACTTCGAA ACCATTAAGC TCATCAGCAA TGGCGCCTAT 
GGGGCCGTGT TCCTGGTGCG GCACAAGTCC ACCCGGCAGC GCTTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTGATCCT GCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGGGACATC 
CTGACTTTTG CTGAGAACCC CTTTGTGGTC AGCATGTTCT GCTCCTTTGA AACCAAGCGC 
CACCTGTGCA TGGTGATGGA GTATGTGGAA GGAGGAGACT GTGCCACGCT GCTGAAGAAC 
ATCGGGGCCC TGCCTGTGGA CATGGTGCGC CTGTACTTTG CAGAAACTGT GCTAGCCCTG 
GAGTACTTAC ACAATTACGG CATCGTGCAC CGTGACCTCA AGCCTGACAA CCTGCTGATT 
ACATCCATGG GTCACATCAA GCTCACTGAT TTTGGCCTTT CCAAAATTGG CCTTATGAGT 
CTGACCACAA ACTTGTACGA GGGCCACATT GAAAAGGATG CACGGGAGTT CCTGGACAAG 
CAGGTATGCG GGACCCCGGA GTACATTGCA CCTGAGGTGA TCCTGCGGCA GGGCTACGGG 
AAGCCAGTGG ACTGGTGGGC TATGGGCATC ATCCTGTACG AGTTCCTGGT GGGCTGCGTC 
CCTTTCTTTG GAGACACTCC GGAGGAGCTC TTTGGGCAAG TGATCAGTGA TGAGATTGTG 
TGGCCTGAGG GGGACGATGC ACTGCCGCCG GACGCCCAAG ATCTCACCTC AAAACTGCTC 
CACCAGAACC CATTGGAGAG ACTGGGCACA GGCAGTGCCT ATGAGGTGAA GCAGCACCCA 
TTCTTCACTG GTCTGGACTG GACGGGACTT CTTCGCCAGA AGGCTGAATT TATTCCTCAG 
CTGGAGTCAG AGGATGATAC CAGCTACTTT GACACCCGCT CAGAGCGATA CCACCACGTG 
GACTCGGAGG ATGAGGAGGA AGTGAGTGAG GATGGCTGCC TTGAGATTCG CCAGTTCTCG 
TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC AGCAGCATGG AGCGGCTCTC GCTGCTCGAG 
GAGCGCCGGA CGCCACCCCC CACCAAGCGC AGCCTGAGTG AGGAAAAGGA GGACCGCACG 
GATGGCCTGG CGGGGCTGAG GGGCCGAGAG CGAAGCTGGG TGATCGGCTC CCCTGAGATA 
TTACGGAAGC GGCTGTCGGT GTCCGAGTCG TCCCACACGG AAAGCGATTC CAGCCCTCCA 
CTCACCGTGC GGCGCCACTG CTCGGGCCTC CTGGACGTGC CTCGCTTCCC TGAGAGCACT 
GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG CAGCAGGAGG GTGCATGGCT TCTGACCCCG 
TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG GAACGGCCGG CCGAGCGGCG GCTGAAGCTG 
AATGAGGAAC CTGCTGGCCA GAGCAGCGCC CCCAGCCCAG CCATGGAGAC CCGAGGAGGC 
CGCGGAACCC CACAGCTGGC AGAAGGAGCC ACAGCCAGGG CCATCAGCGA CCTGGCGGTG 
CGCAGGGCCC GCCACCGGCT GCTCTCTGGG GACTCCATAG AAAAGCGCAC CGCTCGCCCC 
ATCAACAAAG TGATCAAGTC CGCCTCAGCC ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG 
CACCACTCCT GCTCTCCACT GGCCAGCCCC ATGTCCCCAC ATTCCCAGTC ATCCAACCCA 
TCATCCAGGG ACTCTTCTCC AAGCAGGGAC TTCCTGCCAG CCCTCAGCAG CTCACGGCCC 
CCCATTGTCA TCCACCGAGC CGGCAAGAAG TATGGCTTCA CCCTACGAGC CATCCGTGTC 
TACATGGGTG ACTCAGATGT CTACACCGTG CACCACATGG TGTGGCACGT GGAGGACGGA 
GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG GGGGACCTCA TCACGCACGT CAACGGGGAG 
CCTGTGCACG GGCTGGTGCA CACGGAGGTG GTGGAGCTGA TCCTGAAGAG TGGAAACAAG 
GTGTCTATTT CAACAACTCC CCTGGAGAAC ACCTCCATTA AGGTGGGGCC CGCTCGGAAG 
GGCAGCTACA AGGCCAAGAT GGCCCGAAGG AGCAAGCGGA GCCGGGGCAA GGATGGACAA 
GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG AAGATCACCA AGCAGGCATC CCTGCTCCAC 
ACCAGCCGCA GCCTTTCTTC CCTTAACCGC TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC 
TCGCCCACAC ACAGCCACAG CCTTTCTCCC CGCTCTCCCA CGCAGGGCTA CCGGGTGACC 
CCTGAAGCCG TGCACTCAGT GGGGGGGAAT TCATCACAGA GCAGCTCCCC CAGCTCCAGC 
GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC ACACGGCCGA GCTCCCTCCA CGGTCTGGCG 
CCCAAGCTCC AACGCCAGTA CCGCTCTCCA CGGCGCAAGT CAGCAGGCAG CATCCCGCTG 
TCACCACTGG CCCACACCCC TTCACCACCA CCAGCGGCTT CGCCTCAGCG TTCCCCATCA 
CCTCTGTCTG GCCACGGGGC CCAGGCCTTC CCTACCAAGC TCCACTTGTC GCCTCCCCTA 
GGCCGGCAAC TCTCACGGCC CAAGAGCGCG GAGCCACCCC GCTCACCACT ACTTAAGAGG 
GTGCAGTCAG CTGAAAAACT GGCAGCTGCA CTGGCTGCTT CTGAGAAGAA GCTCGCCACT 
TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC CCCGAGCTAA AGAAGGAATT GCCGCCCAGG 
GAAGTCAGCT CTCTGGAGGT AGTTGGGACC AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG 
CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC TCACGGGCCT TAGGGACCCT CCGCCAGGAC 
AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG CAGGAAGCCA TCCGGGAGGT TGACTCTTCA 
GAAGATGATA CTGATGAGGG GCCTGAGAAC AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA 
CCCCACCGAG AAGGGGGCCA GGATCCACCC CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG 
GATGCCTTCT TGCCCAGGGA TCCCAGGGGA CAGGGCCCAG TGGTCACAGG TCTACTGACA 
GGGGTCACAC TGGGATCTCC CAGAATGGAA GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA 
ATCCTACAGG CTTTTGAAGA GGGTTCCAGC TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG 
GCTGGCCCCA CAGACCCCAT CCCCGCTGAA GGCTGCTGGA AGGCCCAGCA CCTCCACACC 
CAGGCACTGA CAGCACTTTG TCCGAGCTCA TCAGGACGCA TCCCTGCCAG TTGCTCCACT 
GCCTCCACCC CTGGAGAGCT GGGCCCATGG TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC 
AGGGCATACC CAACCGGAAA GGCAGCAATG GAAGATGGGC CAGCCAGCTC CCAAGATTTG 
GAAACTACAA CTCCAGGCCA CCCTGCAAAC CTGTCCCCCA GACAGGAGGG GAAGTCATGG 
CCACCCAGTG CCCCTGGGCT GGCCCATCCA CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA 
TGGGAGTCTG AGTGTGCACA GAGAGAGAAA GAGGAGCCAG CGCTGGGCAT CACCAAAGTG 
CCTGATGCCT CAGGTGACAG GAGGCAGGAC GTTCCATGCA AGAGCTGCCC CTTCACCCCA 
GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC CAAGACCCAG GGGGCCCTCA GGAGCATCAG 
GACTTGGCAT TGTTACCTGA TGAGCTTTTA AAGCAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_005204886.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204943.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X9, mRNA.

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CloneID	OBa18165
Clone ID Related Accession (Same CDS sequence)	XM_005204886.3 , XM_005204886.4 , XM_015462790.1
Accession Version	XM_005204886.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204886.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA CTGTCGGACA 
AGTAACCGCA AGAGTTTGAT TGTGACCTCT AGTACGTCAC CTACACTGCC ACGGCCACAC 
TCACCACTCC ATGGCCACAC AGGTAACAGT CCTTTGGATA GTCCCCGGAA TTTCTCTCCA 
AATGCACCTG CTCACTTTTC CTTTGTTCCT GCCCGTAGCC ATGGCCACAG AGCAGACAGG 
ACTGATGGAC GGCGCTGGTC TTTGGCCTCA TTGCCTTCTT CAGGCTATGG AACCAACACC 
CCTAGTTCTA CCGTGTCATC GTCGTGCTCC TCTCAGGAGA AGCTGCACCA GTTGCCTTTC 
CAGCCCACGG CCGATGAGCT GCACTTCTTG ACGAAGCATT TCAGCACAGA CAGTGTGCCT 
GATGAGGAGG GGCGGCAGTC CCCCGCCCTG CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA 
CGGTCACCAG TTTCCTTTGA CAGTGAAATA ATAATGATGA ATCATGTATA CAAGGAAAGA 
TTCCCCAAGG CCACTGCACA AATGGAAGAG CGTCTGGCCG AGTTCATCTC CTCCAATACT 
CCAGACAACG TGTTGCCCTT GGCTGATGGA ACCTTGAGCT TTATTCATCA TCAGGTGATA 
GAGATGGCCC GAGACTGCCT GGACAAATCC CGGAGTGGCC TCATTACCTC ACACTACTTC 
TACGAACTTC AAGAGAATTT AGAGAAGCTT CTGCAAGATG CTCATGAGCG CTCAGAGAGT 
TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG AAAAAGCTGA TGATTGTCAT TGCCCGCCCA 
GCTCGTCTCC TGGAATGCCT GGAGTTTGAC CCTGAAGAGT TCTACCACCT GTTAGAAGCA 
GCTGAGGGCC ACGCCAAAGA GGGACAAGGG ATTAAATGTG ACATTCCCCG CTACATCGTT 
AGCCAACTGG GCCTCACACG GGATCCCCTA GAGGAAATGG CCCAGTTGAG CAGCTATGAC 
AGTCCTGATA CTCCGGAGAC AGATGATTCA GTCGAGGGCC GTGGGGCATC TCTGACATCG 
AAAAAGACAC CCTCTGAGGA GGACTTCGAA ACCATTAAGC TCATCAGCAA TGGCGCCTAT 
GGGGCCGTGT TCCTGGTGCG GCACAAGTCC ACCCGGCAGC GCTTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTGATCCT GCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGGGACATC 
CTGACTTTTG CTGAGAACCC CTTTGTGGTC AGCATGTTCT GCTCCTTTGA AACCAAGCGC 
CACCTGTGCA TGGTGATGGA GTATGTGGAA GGAGGAGACT GTGCCACGCT GCTGAAGAAC 
ATCGGGGCCC TGCCTGTGGA CATGGTGCGC CTGTACTTTG CAGAAACTGT GCTAGCCCTG 
GAGTACTTAC ACAATTACGG CATCGTGCAC CGTGACCTCA AGCCTGACAA CCTGCTGATT 
ACATCCATGG GTCACATCAA GCTCACTGAT TTTGGCCTTT CCAAAATTGG CCTTATGAGT 
CTGACCACAA ACTTGTACGA GGGCCACATT GAAAAGGATG CACGGGAGTT CCTGGACAAG 
CAGGTATGCG GGACCCCGGA GTACATTGCA CCTGAGGTGA TCCTGCGGCA GGGCTACGGG 
AAGCCAGTGG ACTGGTGGGC TATGGGCATC ATCCTGTACG AGTTCCTGGT GGGCTGCGTC 
CCTTTCTTTG GAGACACTCC GGAGGAGCTC TTTGGGCAAG TGATCAGTGA TGAGATTGTG 
TGGCCTGAGG GGGACGATGC ACTGCCGCCG GACGCCCAAG ATCTCACCTC AAAACTGCTC 
CACCAGAACC CATTGGAGAG ACTGGGCACA GGCAGTGCCT ATGAGGTGAA GCAGCACCCA 
TTCTTCACTG GTCTGGACTG GACGGGACTT CTTCGCCAGA AGGCTGAATT TATTCCTCAG 
CTGGAGTCAG AGGATGATAC CAGCTACTTT GACACCCGCT CAGAGCGATA CCACCACGTG 
GACTCGGAGG ATGAGGAGGA AGTGAGTGAG GATGGCTGCC TTGAGATTCG CCAGTTCTCG 
TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC AGCAGCATGG AGCGGCTCTC GCTGCTCGAG 
GAGCGCCGGA CGCCACCCCC CACCAAGCGC AGCCTGAGTG AGGAAAAGGA GGACCGCACG 
GATGGCCTGG CGGGGCTGAG GGGCCGAGAG CGAAGCTGGG TGATCGGCTC CCCTGAGATA 
TTACGGAAGC GGCTGTCGGT GTCCGAGTCG TCCCACACGG AAAGCGATTC CAGCCCTCCA 
CTCACCGTGC GGCGCCACTG CTCGGGCCTC CTGGACGTGC CTCGCTTCCC TGAGAGCACT 
GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG CAGCAGGAGG GTGCATGGCT TCTGACCCCG 
TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG GAACGGCCGG CCGAGCGGCG GCTGAAGCTG 
AATGAGGAAC CTGCTGGCCA GAGCAGCGCC CCCAGCCCAG CCATGGAGAC CCGAGGAGGC 
CGCGGAACCC CACAGCTGGC AGAAGGAGCC ACAGCCAGGG CCATCAGCGA CCTGGCGGTG 
CGCAGGGCCC GCCACCGGCT GCTCTCTGGG GACTCCATAG AAAAGCGCAC CGCTCGCCCC 
ATCAACAAAG TGATCAAGTC CGCCTCAGCC ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG 
CACCACTCCT GCTCTCCACT GGCCAGCCCC ATGTCCCCAC ATTCCCAGTC ATCCAACCCA 
TCATCCAGGG ACTCTTCTCC AAGCAGGGAC TTCCTGCCAG CCCTCAGCAG CTCACGGCCC 
CCCATTGTCA TCCACCGAGC CGGCAAGAAG TATGGCTTCA CCCTACGAGC CATCCGTGTC 
TACATGGGTG ACTCAGATGT CTACACCGTG CACCACATGG TGTGGCACGT GGAGGACGGA 
GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG GGGGACCTCA TCACGCACGT CAACGGGGAG 
CCTGTGCACG GGCTGGTGCA CACGGAGGTG GTGGAGCTGA TCCTGAAGAG TGGAAACAAG 
GTGTCTATTT CAACAACTCC CCTGGAGAAC ACCTCCATTA AGGTGGGGCC CGCTCGGAAG 
GGCAGCTACA AGGCCAAGAT GGCCCGAAGG AGCAAGCGGA GCCGGGGCAA GGATGGACAA 
GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG AAGATCACCA AGCAGGCATC CCTGCTCCAC 
ACCAGCCGCA GCCTTTCTTC CCTTAACCGC TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC 
TCGCCCACAC ACAGCCACAG CCTTTCTCCC CGCTCTCCCA CGCAGGGCTA CCGGGTGACC 
CCTGAAGCCG TGCACTCAGT GGGGGGGAAT TCATCACAGA GCAGCTCCCC CAGCTCCAGC 
GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC ACACGGCCGA GCTCCCTCCA CGGTCTGGCG 
CCCAAGCTCC AACGCCAGTA CCGCTCTCCA CGGCGCAAGT CAGCAGGCAG CATCCCGCTG 
TCACCACTGG CCCACACCCC TTCACCACCA CCAGCGGCTT CGCCTCAGCG TTCCCCATCA 
CCTCTGTCTG GCCACGGGGC CCAGGCCTTC CCTACCAAGC TCCACTTGTC GCCTCCCCTA 
GGCCGGCAAC TCTCACGGCC CAAGAGCGCG GAGCCACCCC GCTCACCACT ACTTAAGAGG 
GTGCAGTCAG CTGAAAAACT GGCAGCTGCA CTGGCTGCTT CTGAGAAGAA GCTCGCCACT 
TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC CCCGAGCTAA AGAAGGAATT GCCGCCCAGG 
GAAGTCAGCT CTCTGGAGGT AGTTGGGACC AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG 
CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC TCACGGGCCT TAGGGACCCT CCGCCAGGAC 
AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG CAGGAAGCCA TCCGGGAGGT TGACTCTTCA 
GAAGATGATA CTGATGAGGG GCCTGAGAAC AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA 
CCCCACCGAG AAGGGGGCCA GGATCCACCC CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG 
GATGCCTTCT TGCCCAGGGA TCCCAGGGGA CAGGGCCCAG TGGTCACAGG TCTACTGACA 
GGGGTCACAC TGGGATCTCC CAGAATGGAA GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA 
ATCCTACAGG CTTTTGAAGA GGGTTCCAGC TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG 
GCTGGCCCCA CAGACCCCAT CCCCGCTGAA GGCTGCTGGA AGGCCCAGCA CCTCCACACC 
CAGGCACTGA CAGCACTTTG TCCGAGCTCA TCAGGACGCA TCCCTGCCAG TTGCTCCACT 
GCCTCCACCC CTGGAGAGCT GGGCCCATGG TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC 
AGGGCATACC CAACCGGAAA GGCAGCAATG GAAGATGGGC CAGCCAGCTC CCAAGATTTG 
GAAACTACAA CTCCAGGCCA CCCTGCAAAC CTGTCCCCCA GACAGGAGGG GAAGTCATGG 
CCACCCAGTG CCCCTGGGCT GGCCCATCCA CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA 
TGGGAGTCTG AGTGTGCACA GAGAGAGAAA GAGGAGCCAG CGCTGGGCAT CACCAAAGTG 
CCTGATGCCT CAGGTGACAG GAGGCAGGAC GTTCCATGCA AGAGCTGCCC CTTCACCCCA 
GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC CAAGACCCAG GGGGCCCTCA GGAGCATCAG 
GACTTGGCAT TGTTACCTGA TGAGCTTTTA AAGCAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204943.1
CDS	242..5203
Translation	MMKRRRERLGAPCLRIHCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_005204886.4
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204886.4

ORF Insert Sequence:

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA CTGTCGGACA 
AGTAACCGCA AGAGTTTGAT TGTGACCTCT AGTACGTCAC CTACACTGCC ACGGCCACAC 
TCACCACTCC ATGGCCACAC AGGTAACAGT CCTTTGGATA GTCCCCGGAA TTTCTCTCCA 
AATGCACCTG CTCACTTTTC CTTTGTTCCT GCCCGTAGCC ATGGCCACAG AGCAGACAGG 
ACTGATGGAC GGCGCTGGTC TTTGGCCTCA TTGCCTTCTT CAGGCTATGG AACCAACACC 
CCTAGTTCTA CCGTGTCATC GTCGTGCTCC TCTCAGGAGA AGCTGCACCA GTTGCCTTTC 
CAGCCCACGG CCGATGAGCT GCACTTCTTG ACGAAGCATT TCAGCACAGA CAGTGTGCCT 
GATGAGGAGG GGCGGCAGTC CCCCGCCCTG CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA 
CGGTCACCAG TTTCCTTTGA CAGTGAAATA ATAATGATGA ATCATGTATA CAAGGAAAGA 
TTCCCCAAGG CCACTGCACA AATGGAAGAG CGTCTGGCCG AGTTCATCTC CTCCAATACT 
CCAGACAACG TGTTGCCCTT GGCTGATGGA ACCTTGAGCT TTATTCATCA TCAGGTGATA 
GAGATGGCCC GAGACTGCCT GGACAAATCC CGGAGTGGCC TCATTACCTC ACACTACTTC 
TACGAACTTC AAGAGAATTT AGAGAAGCTT CTGCAAGATG CTCATGAGCG CTCAGAGAGT 
TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG AAAAAGCTGA TGATTGTCAT TGCCCGCCCA 
GCTCGTCTCC TGGAATGCCT GGAGTTTGAC CCTGAAGAGT TCTACCACCT GTTAGAAGCA 
GCTGAGGGCC ACGCCAAAGA GGGACAAGGG ATTAAATGTG ACATTCCCCG CTACATCGTT 
AGCCAACTGG GCCTCACACG GGATCCCCTA GAGGAAATGG CCCAGTTGAG CAGCTATGAC 
AGTCCTGATA CTCCGGAGAC AGATGATTCA GTCGAGGGCC GTGGGGCATC TCTGACATCG 
AAAAAGACAC CCTCTGAGGA GGACTTCGAA ACCATTAAGC TCATCAGCAA TGGCGCCTAT 
GGGGCCGTGT TCCTGGTGCG GCACAAGTCC ACCCGGCAGC GCTTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTGATCCT GCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGGGACATC 
CTGACTTTTG CTGAGAACCC CTTTGTGGTC AGCATGTTCT GCTCCTTTGA AACCAAGCGC 
CACCTGTGCA TGGTGATGGA GTATGTGGAA GGAGGAGACT GTGCCACGCT GCTGAAGAAC 
ATCGGGGCCC TGCCTGTGGA CATGGTGCGC CTGTACTTTG CAGAAACTGT GCTAGCCCTG 
GAGTACTTAC ACAATTACGG CATCGTGCAC CGTGACCTCA AGCCTGACAA CCTGCTGATT 
ACATCCATGG GTCACATCAA GCTCACTGAT TTTGGCCTTT CCAAAATTGG CCTTATGAGT 
CTGACCACAA ACTTGTACGA GGGCCACATT GAAAAGGATG CACGGGAGTT CCTGGACAAG 
CAGGTATGCG GGACCCCGGA GTACATTGCA CCTGAGGTGA TCCTGCGGCA GGGCTACGGG 
AAGCCAGTGG ACTGGTGGGC TATGGGCATC ATCCTGTACG AGTTCCTGGT GGGCTGCGTC 
CCTTTCTTTG GAGACACTCC GGAGGAGCTC TTTGGGCAAG TGATCAGTGA TGAGATTGTG 
TGGCCTGAGG GGGACGATGC ACTGCCGCCG GACGCCCAAG ATCTCACCTC AAAACTGCTC 
CACCAGAACC CATTGGAGAG ACTGGGCACA GGCAGTGCCT ATGAGGTGAA GCAGCACCCA 
TTCTTCACTG GTCTGGACTG GACGGGACTT CTTCGCCAGA AGGCTGAATT TATTCCTCAG 
CTGGAGTCAG AGGATGATAC CAGCTACTTT GACACCCGCT CAGAGCGATA CCACCACGTG 
GACTCGGAGG ATGAGGAGGA AGTGAGTGAG GATGGCTGCC TTGAGATTCG CCAGTTCTCG 
TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC AGCAGCATGG AGCGGCTCTC GCTGCTCGAG 
GAGCGCCGGA CGCCACCCCC CACCAAGCGC AGCCTGAGTG AGGAAAAGGA GGACCGCACG 
GATGGCCTGG CGGGGCTGAG GGGCCGAGAG CGAAGCTGGG TGATCGGCTC CCCTGAGATA 
TTACGGAAGC GGCTGTCGGT GTCCGAGTCG TCCCACACGG AAAGCGATTC CAGCCCTCCA 
CTCACCGTGC GGCGCCACTG CTCGGGCCTC CTGGACGTGC CTCGCTTCCC TGAGAGCACT 
GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG CAGCAGGAGG GTGCATGGCT TCTGACCCCG 
TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG GAACGGCCGG CCGAGCGGCG GCTGAAGCTG 
AATGAGGAAC CTGCTGGCCA GAGCAGCGCC CCCAGCCCAG CCATGGAGAC CCGAGGAGGC 
CGCGGAACCC CACAGCTGGC AGAAGGAGCC ACAGCCAGGG CCATCAGCGA CCTGGCGGTG 
CGCAGGGCCC GCCACCGGCT GCTCTCTGGG GACTCCATAG AAAAGCGCAC CGCTCGCCCC 
ATCAACAAAG TGATCAAGTC CGCCTCAGCC ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG 
CACCACTCCT GCTCTCCACT GGCCAGCCCC ATGTCCCCAC ATTCCCAGTC ATCCAACCCA 
TCATCCAGGG ACTCTTCTCC AAGCAGGGAC TTCCTGCCAG CCCTCAGCAG CTCACGGCCC 
CCCATTGTCA TCCACCGAGC CGGCAAGAAG TATGGCTTCA CCCTACGAGC CATCCGTGTC 
TACATGGGTG ACTCAGATGT CTACACCGTG CACCACATGG TGTGGCACGT GGAGGACGGA 
GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG GGGGACCTCA TCACGCACGT CAACGGGGAG 
CCTGTGCACG GGCTGGTGCA CACGGAGGTG GTGGAGCTGA TCCTGAAGAG TGGAAACAAG 
GTGTCTATTT CAACAACTCC CCTGGAGAAC ACCTCCATTA AGGTGGGGCC CGCTCGGAAG 
GGCAGCTACA AGGCCAAGAT GGCCCGAAGG AGCAAGCGGA GCCGGGGCAA GGATGGACAA 
GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG AAGATCACCA AGCAGGCATC CCTGCTCCAC 
ACCAGCCGCA GCCTTTCTTC CCTTAACCGC TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC 
TCGCCCACAC ACAGCCACAG CCTTTCTCCC CGCTCTCCCA CGCAGGGCTA CCGGGTGACC 
CCTGAAGCCG TGCACTCAGT GGGGGGGAAT TCATCACAGA GCAGCTCCCC CAGCTCCAGC 
GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC ACACGGCCGA GCTCCCTCCA CGGTCTGGCG 
CCCAAGCTCC AACGCCAGTA CCGCTCTCCA CGGCGCAAGT CAGCAGGCAG CATCCCGCTG 
TCACCACTGG CCCACACCCC TTCACCACCA CCAGCGGCTT CGCCTCAGCG TTCCCCATCA 
CCTCTGTCTG GCCACGGGGC CCAGGCCTTC CCTACCAAGC TCCACTTGTC GCCTCCCCTA 
GGCCGGCAAC TCTCACGGCC CAAGAGCGCG GAGCCACCCC GCTCACCACT ACTTAAGAGG 
GTGCAGTCAG CTGAAAAACT GGCAGCTGCA CTGGCTGCTT CTGAGAAGAA GCTCGCCACT 
TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC CCCGAGCTAA AGAAGGAATT GCCGCCCAGG 
GAAGTCAGCT CTCTGGAGGT AGTTGGGACC AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG 
CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC TCACGGGCCT TAGGGACCCT CCGCCAGGAC 
AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG CAGGAAGCCA TCCGGGAGGT TGACTCTTCA 
GAAGATGATA CTGATGAGGG GCCTGAGAAC AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA 
CCCCACCGAG AAGGGGGCCA GGATCCACCC CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG 
GATGCCTTCT TGCCCAGGGA TCCCAGGGGA CAGGGCCCAG TGGTCACAGG TCTACTGACA 
GGGGTCACAC TGGGATCTCC CAGAATGGAA GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA 
ATCCTACAGG CTTTTGAAGA GGGTTCCAGC TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG 
GCTGGCCCCA CAGACCCCAT CCCCGCTGAA GGCTGCTGGA AGGCCCAGCA CCTCCACACC 
CAGGCACTGA CAGCACTTTG TCCGAGCTCA TCAGGACGCA TCCCTGCCAG TTGCTCCACT 
GCCTCCACCC CTGGAGAGCT GGGCCCATGG TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC 
AGGGCATACC CAACCGGAAA GGCAGCAATG GAAGATGGGC CAGCCAGCTC CCAAGATTTG 
GAAACTACAA CTCCAGGCCA CCCTGCAAAC CTGTCCCCCA GACAGGAGGG GAAGTCATGG 
CCACCCAGTG CCCCTGGGCT GGCCCATCCA CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA 
TGGGAGTCTG AGTGTGCACA GAGAGAGAAA GAGGAGCCAG CGCTGGGCAT CACCAAAGTG 
CCTGATGCCT CAGGTGACAG GAGGCAGGAC GTTCCATGCA AGAGCTGCCC CTTCACCCCA 
GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC CAAGACCCAG GGGGCCCTCA GGAGCATCAG 
GACTTGGCAT TGTTACCTGA TGAGCTTTTA AAGCAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_015462790.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318276.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA.

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CloneID	OBa18165
Clone ID Related Accession (Same CDS sequence)	XM_005204886.3 , XM_005204886.4 , XM_015462790.1
Accession Version	XM_015462790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA CTGTCGGACA 
AGTAACCGCA AGAGTTTGAT TGTGACCTCT AGTACGTCAC CTACACTGCC ACGGCCACAC 
TCACCACTCC ATGGCCACAC AGGTAACAGT CCTTTGGATA GTCCCCGGAA TTTCTCTCCA 
AATGCACCTG CTCACTTTTC CTTTGTTCCT GCCCGTAGCC ATGGCCACAG AGCAGACAGG 
ACTGATGGAC GGCGCTGGTC TTTGGCCTCA TTGCCTTCTT CAGGCTATGG AACCAACACC 
CCTAGTTCTA CCGTGTCATC GTCGTGCTCC TCTCAGGAGA AGCTGCACCA GTTGCCTTTC 
CAGCCCACGG CCGATGAGCT GCACTTCTTG ACGAAGCATT TCAGCACAGA CAGTGTGCCT 
GATGAGGAGG GGCGGCAGTC CCCCGCCCTG CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA 
CGGTCACCAG TTTCCTTTGA CAGTGAAATA ATAATGATGA ATCATGTATA CAAGGAAAGA 
TTCCCCAAGG CCACTGCACA AATGGAAGAG CGTCTGGCCG AGTTCATCTC CTCCAATACT 
CCAGACAACG TGTTGCCCTT GGCTGATGGA ACCTTGAGCT TTATTCATCA TCAGGTGATA 
GAGATGGCCC GAGACTGCCT GGACAAATCC CGGAGTGGCC TCATTACCTC ACACTACTTC 
TACGAACTTC AAGAGAATTT AGAGAAGCTT CTGCAAGATG CTCATGAGCG CTCAGAGAGT 
TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG AAAAAGCTGA TGATTGTCAT TGCCCGCCCA 
GCTCGTCTCC TGGAATGCCT GGAGTTTGAC CCTGAAGAGT TCTACCACCT GTTAGAAGCA 
GCTGAGGGCC ACGCCAAAGA GGGACAAGGG ATTAAATGTG ACATTCCCCG CTACATCGTT 
AGCCAACTGG GCCTCACACG GGATCCCCTA GAGGAAATGG CCCAGTTGAG CAGCTATGAC 
AGTCCTGATA CTCCGGAGAC AGATGATTCA GTCGAGGGCC GTGGGGCATC TCTGACATCG 
AAAAAGACAC CCTCTGAGGA GGACTTCGAA ACCATTAAGC TCATCAGCAA TGGCGCCTAT 
GGGGCCGTGT TCCTGGTGCG GCACAAGTCC ACCCGGCAGC GCTTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTGATCCT GCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGGGACATC 
CTGACTTTTG CTGAGAACCC CTTTGTGGTC AGCATGTTCT GCTCCTTTGA AACCAAGCGC 
CACCTGTGCA TGGTGATGGA GTATGTGGAA GGAGGAGACT GTGCCACGCT GCTGAAGAAC 
ATCGGGGCCC TGCCTGTGGA CATGGTGCGC CTGTACTTTG CAGAAACTGT GCTAGCCCTG 
GAGTACTTAC ACAATTACGG CATCGTGCAC CGTGACCTCA AGCCTGACAA CCTGCTGATT 
ACATCCATGG GTCACATCAA GCTCACTGAT TTTGGCCTTT CCAAAATTGG CCTTATGAGT 
CTGACCACAA ACTTGTACGA GGGCCACATT GAAAAGGATG CACGGGAGTT CCTGGACAAG 
CAGGTATGCG GGACCCCGGA GTACATTGCA CCTGAGGTGA TCCTGCGGCA GGGCTACGGG 
AAGCCAGTGG ACTGGTGGGC TATGGGCATC ATCCTGTACG AGTTCCTGGT GGGCTGCGTC 
CCTTTCTTTG GAGACACTCC GGAGGAGCTC TTTGGGCAAG TGATCAGTGA TGAGATTGTG 
TGGCCTGAGG GGGACGATGC ACTGCCGCCG GACGCCCAAG ATCTCACCTC AAAACTGCTC 
CACCAGAACC CATTGGAGAG ACTGGGCACA GGCAGTGCCT ATGAGGTGAA GCAGCACCCA 
TTCTTCACTG GTCTGGACTG GACGGGACTT CTTCGCCAGA AGGCTGAATT TATTCCTCAG 
CTGGAGTCAG AGGATGATAC CAGCTACTTT GACACCCGCT CAGAGCGATA CCACCACGTG 
GACTCGGAGG ATGAGGAGGA AGTGAGTGAG GATGGCTGCC TTGAGATTCG CCAGTTCTCG 
TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC AGCAGCATGG AGCGGCTCTC GCTGCTCGAG 
GAGCGCCGGA CGCCACCCCC CACCAAGCGC AGCCTGAGTG AGGAAAAGGA GGACCGCACG 
GATGGCCTGG CGGGGCTGAG GGGCCGAGAG CGAAGCTGGG TGATCGGCTC CCCTGAGATA 
TTACGGAAGC GGCTGTCGGT GTCCGAGTCG TCCCACACGG AAAGCGATTC CAGCCCTCCA 
CTCACCGTGC GGCGCCACTG CTCGGGCCTC CTGGACGTGC CTCGCTTCCC TGAGAGCACT 
GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG CAGCAGGAGG GTGCATGGCT TCTGACCCCG 
TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG GAACGGCCGG CCGAGCGGCG GCTGAAGCTG 
AATGAGGAAC CTGCTGGCCA GAGCAGCGCC CCCAGCCCAG CCATGGAGAC CCGAGGAGGC 
CGCGGAACCC CACAGCTGGC AGAAGGAGCC ACAGCCAGGG CCATCAGCGA CCTGGCGGTG 
CGCAGGGCCC GCCACCGGCT GCTCTCTGGG GACTCCATAG AAAAGCGCAC CGCTCGCCCC 
ATCAACAAAG TGATCAAGTC CGCCTCAGCC ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG 
CACCACTCCT GCTCTCCACT GGCCAGCCCC ATGTCCCCAC ATTCCCAGTC ATCCAACCCA 
TCATCCAGGG ACTCTTCTCC AAGCAGGGAC TTCCTGCCAG CCCTCAGCAG CTCACGGCCC 
CCCATTGTCA TCCACCGAGC CGGCAAGAAG TATGGCTTCA CCCTACGAGC CATCCGTGTC 
TACATGGGTG ACTCAGATGT CTACACCGTG CACCACATGG TGTGGCACGT GGAGGACGGA 
GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG GGGGACCTCA TCACGCACGT CAACGGGGAG 
CCTGTGCACG GGCTGGTGCA CACGGAGGTG GTGGAGCTGA TCCTGAAGAG TGGAAACAAG 
GTGTCTATTT CAACAACTCC CCTGGAGAAC ACCTCCATTA AGGTGGGGCC CGCTCGGAAG 
GGCAGCTACA AGGCCAAGAT GGCCCGAAGG AGCAAGCGGA GCCGGGGCAA GGATGGACAA 
GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG AAGATCACCA AGCAGGCATC CCTGCTCCAC 
ACCAGCCGCA GCCTTTCTTC CCTTAACCGC TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC 
TCGCCCACAC ACAGCCACAG CCTTTCTCCC CGCTCTCCCA CGCAGGGCTA CCGGGTGACC 
CCTGAAGCCG TGCACTCAGT GGGGGGGAAT TCATCACAGA GCAGCTCCCC CAGCTCCAGC 
GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC ACACGGCCGA GCTCCCTCCA CGGTCTGGCG 
CCCAAGCTCC AACGCCAGTA CCGCTCTCCA CGGCGCAAGT CAGCAGGCAG CATCCCGCTG 
TCACCACTGG CCCACACCCC TTCACCACCA CCAGCGGCTT CGCCTCAGCG TTCCCCATCA 
CCTCTGTCTG GCCACGGGGC CCAGGCCTTC CCTACCAAGC TCCACTTGTC GCCTCCCCTA 
GGCCGGCAAC TCTCACGGCC CAAGAGCGCG GAGCCACCCC GCTCACCACT ACTTAAGAGG 
GTGCAGTCAG CTGAAAAACT GGCAGCTGCA CTGGCTGCTT CTGAGAAGAA GCTCGCCACT 
TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC CCCGAGCTAA AGAAGGAATT GCCGCCCAGG 
GAAGTCAGCT CTCTGGAGGT AGTTGGGACC AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG 
CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC TCACGGGCCT TAGGGACCCT CCGCCAGGAC 
AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG CAGGAAGCCA TCCGGGAGGT TGACTCTTCA 
GAAGATGATA CTGATGAGGG GCCTGAGAAC AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA 
CCCCACCGAG AAGGGGGCCA GGATCCACCC CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG 
GATGCCTTCT TGCCCAGGGA TCCCAGGGGA CAGGGCCCAG TGGTCACAGG TCTACTGACA 
GGGGTCACAC TGGGATCTCC CAGAATGGAA GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA 
ATCCTACAGG CTTTTGAAGA GGGTTCCAGC TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG 
GCTGGCCCCA CAGACCCCAT CCCCGCTGAA GGCTGCTGGA AGGCCCAGCA CCTCCACACC 
CAGGCACTGA CAGCACTTTG TCCGAGCTCA TCAGGACGCA TCCCTGCCAG TTGCTCCACT 
GCCTCCACCC CTGGAGAGCT GGGCCCATGG TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC 
AGGGCATACC CAACCGGAAA GGCAGCAATG GAAGATGGGC CAGCCAGCTC CCAAGATTTG 
GAAACTACAA CTCCAGGCCA CCCTGCAAAC CTGTCCCCCA GACAGGAGGG GAAGTCATGG 
CCACCCAGTG CCCCTGGGCT GGCCCATCCA CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA 
TGGGAGTCTG AGTGTGCACA GAGAGAGAAA GAGGAGCCAG CGCTGGGCAT CACCAAAGTG 
CCTGATGCCT CAGGTGACAG GAGGCAGGAC GTTCCATGCA AGAGCTGCCC CTTCACCCCA 
GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC CAAGACCCAG GGGGCCCTCA GGAGCATCAG 
GACTTGGCAT TGTTACCTGA TGAGCTTTTA AAGCAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318276.1
CDS	263..5224
Translation	MMKRRRERLGAPCLRIHCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_015462790.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462790.1

ORF Insert Sequence:

ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA CTGTCGGACA 
AGTAACCGCA AGAGTTTGAT TGTGACCTCT AGTACGTCAC CTACACTGCC ACGGCCACAC 
TCACCACTCC ATGGCCACAC AGGTAACAGT CCTTTGGATA GTCCCCGGAA TTTCTCTCCA 
AATGCACCTG CTCACTTTTC CTTTGTTCCT GCCCGTAGCC ATGGCCACAG AGCAGACAGG 
ACTGATGGAC GGCGCTGGTC TTTGGCCTCA TTGCCTTCTT CAGGCTATGG AACCAACACC 
CCTAGTTCTA CCGTGTCATC GTCGTGCTCC TCTCAGGAGA AGCTGCACCA GTTGCCTTTC 
CAGCCCACGG CCGATGAGCT GCACTTCTTG ACGAAGCATT TCAGCACAGA CAGTGTGCCT 
GATGAGGAGG GGCGGCAGTC CCCCGCCCTG CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA 
CGGTCACCAG TTTCCTTTGA CAGTGAAATA ATAATGATGA ATCATGTATA CAAGGAAAGA 
TTCCCCAAGG CCACTGCACA AATGGAAGAG CGTCTGGCCG AGTTCATCTC CTCCAATACT 
CCAGACAACG TGTTGCCCTT GGCTGATGGA ACCTTGAGCT TTATTCATCA TCAGGTGATA 
GAGATGGCCC GAGACTGCCT GGACAAATCC CGGAGTGGCC TCATTACCTC ACACTACTTC 
TACGAACTTC AAGAGAATTT AGAGAAGCTT CTGCAAGATG CTCATGAGCG CTCAGAGAGT 
TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG AAAAAGCTGA TGATTGTCAT TGCCCGCCCA 
GCTCGTCTCC TGGAATGCCT GGAGTTTGAC CCTGAAGAGT TCTACCACCT GTTAGAAGCA 
GCTGAGGGCC ACGCCAAAGA GGGACAAGGG ATTAAATGTG ACATTCCCCG CTACATCGTT 
AGCCAACTGG GCCTCACACG GGATCCCCTA GAGGAAATGG CCCAGTTGAG CAGCTATGAC 
AGTCCTGATA CTCCGGAGAC AGATGATTCA GTCGAGGGCC GTGGGGCATC TCTGACATCG 
AAAAAGACAC CCTCTGAGGA GGACTTCGAA ACCATTAAGC TCATCAGCAA TGGCGCCTAT 
GGGGCCGTGT TCCTGGTGCG GCACAAGTCC ACCCGGCAGC GCTTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTGATCCT GCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGGGACATC 
CTGACTTTTG CTGAGAACCC CTTTGTGGTC AGCATGTTCT GCTCCTTTGA AACCAAGCGC 
CACCTGTGCA TGGTGATGGA GTATGTGGAA GGAGGAGACT GTGCCACGCT GCTGAAGAAC 
ATCGGGGCCC TGCCTGTGGA CATGGTGCGC CTGTACTTTG CAGAAACTGT GCTAGCCCTG 
GAGTACTTAC ACAATTACGG CATCGTGCAC CGTGACCTCA AGCCTGACAA CCTGCTGATT 
ACATCCATGG GTCACATCAA GCTCACTGAT TTTGGCCTTT CCAAAATTGG CCTTATGAGT 
CTGACCACAA ACTTGTACGA GGGCCACATT GAAAAGGATG CACGGGAGTT CCTGGACAAG 
CAGGTATGCG GGACCCCGGA GTACATTGCA CCTGAGGTGA TCCTGCGGCA GGGCTACGGG 
AAGCCAGTGG ACTGGTGGGC TATGGGCATC ATCCTGTACG AGTTCCTGGT GGGCTGCGTC 
CCTTTCTTTG GAGACACTCC GGAGGAGCTC TTTGGGCAAG TGATCAGTGA TGAGATTGTG 
TGGCCTGAGG GGGACGATGC ACTGCCGCCG GACGCCCAAG ATCTCACCTC AAAACTGCTC 
CACCAGAACC CATTGGAGAG ACTGGGCACA GGCAGTGCCT ATGAGGTGAA GCAGCACCCA 
TTCTTCACTG GTCTGGACTG GACGGGACTT CTTCGCCAGA AGGCTGAATT TATTCCTCAG 
CTGGAGTCAG AGGATGATAC CAGCTACTTT GACACCCGCT CAGAGCGATA CCACCACGTG 
GACTCGGAGG ATGAGGAGGA AGTGAGTGAG GATGGCTGCC TTGAGATTCG CCAGTTCTCG 
TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC AGCAGCATGG AGCGGCTCTC GCTGCTCGAG 
GAGCGCCGGA CGCCACCCCC CACCAAGCGC AGCCTGAGTG AGGAAAAGGA GGACCGCACG 
GATGGCCTGG CGGGGCTGAG GGGCCGAGAG CGAAGCTGGG TGATCGGCTC CCCTGAGATA 
TTACGGAAGC GGCTGTCGGT GTCCGAGTCG TCCCACACGG AAAGCGATTC CAGCCCTCCA 
CTCACCGTGC GGCGCCACTG CTCGGGCCTC CTGGACGTGC CTCGCTTCCC TGAGAGCACT 
GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG CAGCAGGAGG GTGCATGGCT TCTGACCCCG 
TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG GAACGGCCGG CCGAGCGGCG GCTGAAGCTG 
AATGAGGAAC CTGCTGGCCA GAGCAGCGCC CCCAGCCCAG CCATGGAGAC CCGAGGAGGC 
CGCGGAACCC CACAGCTGGC AGAAGGAGCC ACAGCCAGGG CCATCAGCGA CCTGGCGGTG 
CGCAGGGCCC GCCACCGGCT GCTCTCTGGG GACTCCATAG AAAAGCGCAC CGCTCGCCCC 
ATCAACAAAG TGATCAAGTC CGCCTCAGCC ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG 
CACCACTCCT GCTCTCCACT GGCCAGCCCC ATGTCCCCAC ATTCCCAGTC ATCCAACCCA 
TCATCCAGGG ACTCTTCTCC AAGCAGGGAC TTCCTGCCAG CCCTCAGCAG CTCACGGCCC 
CCCATTGTCA TCCACCGAGC CGGCAAGAAG TATGGCTTCA CCCTACGAGC CATCCGTGTC 
TACATGGGTG ACTCAGATGT CTACACCGTG CACCACATGG TGTGGCACGT GGAGGACGGA 
GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG GGGGACCTCA TCACGCACGT CAACGGGGAG 
CCTGTGCACG GGCTGGTGCA CACGGAGGTG GTGGAGCTGA TCCTGAAGAG TGGAAACAAG 
GTGTCTATTT CAACAACTCC CCTGGAGAAC ACCTCCATTA AGGTGGGGCC CGCTCGGAAG 
GGCAGCTACA AGGCCAAGAT GGCCCGAAGG AGCAAGCGGA GCCGGGGCAA GGATGGACAA 
GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG AAGATCACCA AGCAGGCATC CCTGCTCCAC 
ACCAGCCGCA GCCTTTCTTC CCTTAACCGC TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC 
TCGCCCACAC ACAGCCACAG CCTTTCTCCC CGCTCTCCCA CGCAGGGCTA CCGGGTGACC 
CCTGAAGCCG TGCACTCAGT GGGGGGGAAT TCATCACAGA GCAGCTCCCC CAGCTCCAGC 
GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC ACACGGCCGA GCTCCCTCCA CGGTCTGGCG 
CCCAAGCTCC AACGCCAGTA CCGCTCTCCA CGGCGCAAGT CAGCAGGCAG CATCCCGCTG 
TCACCACTGG CCCACACCCC TTCACCACCA CCAGCGGCTT CGCCTCAGCG TTCCCCATCA 
CCTCTGTCTG GCCACGGGGC CCAGGCCTTC CCTACCAAGC TCCACTTGTC GCCTCCCCTA 
GGCCGGCAAC TCTCACGGCC CAAGAGCGCG GAGCCACCCC GCTCACCACT ACTTAAGAGG 
GTGCAGTCAG CTGAAAAACT GGCAGCTGCA CTGGCTGCTT CTGAGAAGAA GCTCGCCACT 
TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC CCCGAGCTAA AGAAGGAATT GCCGCCCAGG 
GAAGTCAGCT CTCTGGAGGT AGTTGGGACC AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG 
CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC TCACGGGCCT TAGGGACCCT CCGCCAGGAC 
AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG CAGGAAGCCA TCCGGGAGGT TGACTCTTCA 
GAAGATGATA CTGATGAGGG GCCTGAGAAC AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA 
CCCCACCGAG AAGGGGGCCA GGATCCACCC CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG 
GATGCCTTCT TGCCCAGGGA TCCCAGGGGA CAGGGCCCAG TGGTCACAGG TCTACTGACA 
GGGGTCACAC TGGGATCTCC CAGAATGGAA GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA 
ATCCTACAGG CTTTTGAAGA GGGTTCCAGC TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG 
GCTGGCCCCA CAGACCCCAT CCCCGCTGAA GGCTGCTGGA AGGCCCAGCA CCTCCACACC 
CAGGCACTGA CAGCACTTTG TCCGAGCTCA TCAGGACGCA TCCCTGCCAG TTGCTCCACT 
GCCTCCACCC CTGGAGAGCT GGGCCCATGG TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC 
AGGGCATACC CAACCGGAAA GGCAGCAATG GAAGATGGGC CAGCCAGCTC CCAAGATTTG 
GAAACTACAA CTCCAGGCCA CCCTGCAAAC CTGTCCCCCA GACAGGAGGG GAAGTCATGG 
CCACCCAGTG CCCCTGGGCT GGCCCATCCA CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA 
TGGGAGTCTG AGTGTGCACA GAGAGAGAAA GAGGAGCCAG CGCTGGGCAT CACCAAAGTG 
CCTGATGCCT CAGGTGACAG GAGGCAGGAC GTTCCATGCA AGAGCTGCCC CTTCACCCCA 
GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC CAAGACCCAG GGGGCCCTCA GGAGCATCAG 
GACTTGGCAT TGTTACCTGA TGAGCTTTTA AAGCAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_003582019.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003582067.2 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA.

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CloneID	OBa53217
Clone ID Related Accession (Same CDS sequence)	XM_003582019.4 , XM_010803741.2 , XM_010803741.3
Accession Version	XM_003582019.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582019.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATT CCAGTATTCT ACAAAGGAGA GGGTTACAGA AGGAGCTGAG CCTTCCAAGA 
AGAGGCAGCT TCTGTCGGAC AAGTAACCGC AAGAGTTTGA TTGTGACCTC TAGTACGTCA 
CCTACACTGC CACGGCCACA CTCACCACTC CATGGCCACA CAGGTAACAG TCCTTTGGAT 
AGTCCCCGGA ATTTCTCTCC AAATGCACCT GCTCACTTTT CCTTTGTTCC TGCCCGTAGC 
CATGGCCACA GAGCAGACAG GACTGATGGA CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT 
TCAGGCTATG GAACCAACAC CCCTAGTTCT ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG 
AAGCTGCACC AGTTGCCTTT CCAGCCCACG GCCGATGAGC TGCACTTCTT GACGAAGCAT 
TTCAGCACAG ACAGTGTGCC TGATGAGGAG GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC 
TCCCGCAGCC TCAGTCCTGG ACGGTCACCA GTTTCCTTTG ACAGTGAAAT AATAATGATG 
AATCATGTAT ACAAGGAAAG ATTCCCCAAG GCCACTGCAC AAATGGAAGA GCGTCTGGCC 
GAGTTCATCT CCTCCAATAC TCCAGACAAC GTGTTGCCCT TGGCTGATGG AACCTTGAGC 
TTTATTCATC ATCAGGTGAT AGAGATGGCC CGAGACTGCC TGGACAAATC CCGGAGTGGC 
CTCATTACCT CACACTACTT CTACGAACTT CAAGAGAATT TAGAGAAGCT TCTGCAAGAT 
GCTCATGAGC GCTCAGAGAG TTCAGAAGTG GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG 
ATGATTGTCA TTGCCCGCCC AGCTCGTCTC CTGGAATGCC TGGAGTTTGA CCCTGAAGAG 
TTCTACCACC TGTTAGAAGC AGCTGAGGGC CACGCCAAAG AGGGACAAGG GATTAAATGT 
GACATTCCCC GCTACATCGT TAGCCAACTG GGCCTCACAC GGGATCCCCT AGAGGAAATG 
GCCCAGTTGA GCAGCTATGA CAGTCCTGAT ACTCCGGAGA CAGATGATTC AGTCGAGGGC 
CGTGGGGCAT CTCTGACATC GAAAAAGACA CCCTCTGAGG AGGACTTCGA AACCATTAAG 
CTCATCAGCA ATGGCGCCTA TGGGGCCGTG TTCCTGGTGC GGCACAAGTC CACCCGGCAG 
CGCTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTGATCC TGCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGGGACAT CCTGACTTTT GCTGAGAACC CCTTTGTGGT CAGCATGTTC 
TGCTCCTTTG AAACCAAGCG CCACCTGTGC ATGGTGATGG AGTATGTGGA AGGAGGAGAC 
TGTGCCACGC TGCTGAAGAA CATCGGGGCC CTGCCTGTGG ACATGGTGCG CCTGTACTTT 
GCAGAAACTG TGCTAGCCCT GGAGTACTTA CACAATTACG GCATCGTGCA CCGTGACCTC 
AAGCCTGACA ACCTGCTGAT TACATCCATG GGTCACATCA AGCTCACTGA TTTTGGCCTT 
TCCAAAATTG GCCTTATGAG TCTGACCACA AACTTGTACG AGGGCCACAT TGAAAAGGAT 
GCACGGGAGT TCCTGGACAA GCAGGTATGC GGGACCCCGG AGTACATTGC ACCTGAGGTG 
ATCCTGCGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CTATGGGCAT CATCCTGTAC 
GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT GGAGACACTC CGGAGGAGCT CTTTGGGCAA 
GTGATCAGTG ATGAGATTGT GTGGCCTGAG GGGGACGATG CACTGCCGCC GGACGCCCAA 
GATCTCACCT CAAAACTGCT CCACCAGAAC CCATTGGAGA GACTGGGCAC AGGCAGTGCC 
TATGAGGTGA AGCAGCACCC ATTCTTCACT GGTCTGGACT GGACGGGACT TCTTCGCCAG 
AAGGCTGAAT TTATTCCTCA GCTGGAGTCA GAGGATGATA CCAGCTACTT TGACACCCGC 
TCAGAGCGAT ACCACCACGT GGACTCGGAG GATGAGGAGG AAGTGAGTGA GGATGGCTGC 
CTTGAGATTC GCCAGTTCTC GTCCTGTTCT CCAAGGTTCA GCAAGGTGTA CAGCAGCATG 
GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG ACGCCACCCC CCACCAAGCG CAGCCTGAGT 
GAGGAAAAGG AGGACCGCAC GGATGGCCTG GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG 
GTGATCGGCT CCCCTGAGAT ATTACGGAAG CGGCTGTCGG TGTCCGAGTC GTCCCACACG 
GAAAGCGATT CCAGCCCTCC ACTCACCGTG CGGCGCCACT GCTCGGGCCT CCTGGACGTG 
CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG 
GGTGCATGGC TTCTGACCCC GTCTGGAGAG GGGGCGTCTG GGCCTGCCCT GGAACGGCCG 
GCCGAGCGGC GGCTGAAGCT GAATGAGGAA CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA 
GCCATGGAGA CCCGAGGAGG CCGCGGAACC CCACAGCTGG CAGAAGGAGC CACAGCCAGG 
GCCATCAGCG ACCTGGCGGT GCGCAGGGCC CGCCACCGGC TGCTCTCTGG GGACTCCATA 
GAAAAGCGCA CCGCTCGCCC CATCAACAAA GTGATCAAGT CCGCCTCAGC CACAGCCCTG 
TCCCTTCTCA TTCCTGCTGA GCACCACTCC TGCTCTCCAC TGGCCAGCCC CATGTCCCCA 
CATTCCCAGT CATCCAACCC ATCATCCAGG GACTCTTCTC CAAGCAGGGA CTTCCTGCCA 
GCCCTCAGCA GCTCACGGCC CCCCATTGTC ATCCACCGAG CCGGCAAGAA GTATGGCTTC 
ACCCTACGAG CCATCCGTGT CTACATGGGT GACTCAGATG TCTACACCGT GCACCACATG 
GTGTGGCACG TGGAGGACGG AGGTCCAGCC AGTGAGGCAG GGCTGCGTCA GGGGGACCTC 
ATCACGCACG TCAACGGGGA GCCTGTGCAC GGGCTGGTGC ACACGGAGGT GGTGGAGCTG 
ATCCTGAAGA GTGGAAACAA GGTGTCTATT TCAACAACTC CCCTGGAGAA CACCTCCATT 
AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG 
AGCCGGGGCA AGGATGGACA AGAAAGCAGA AAAAGGAGCT CCCTGTTCCG GAAGATCACC 
AAGCAGGCAT CCCTGCTCCA CACCAGCCGC AGCCTTTCTT CCCTTAACCG CTCCCTGTCG 
TCCGGCGAGA GTGGGCCAGG CTCGCCCACA CACAGCCACA GCCTTTCTCC CCGCTCTCCC 
ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC GTGCACTCAG TGGGGGGGAA TTCATCACAG 
AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT TCTCCTGCCG GCTCTGGGCA CACACGGCCG 
AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC CAACGCCAGT ACCGCTCTCC ACGGCGCAAG 
TCAGCAGGCA GCATCCCGCT GTCACCACTG GCCCACACCC CTTCACCACC ACCAGCGGCT 
TCGCCTCAGC GTTCCCCATC ACCTCTGTCT GGCCACGGGG CCCAGGCCTT CCCTACCAAG 
CTCCACTTGT CGCCTCCCCT AGGCCGGCAA CTCTCACGGC CCAAGAGCGC GGAGCCACCC 
CGCTCACCAC TACTTAAGAG GGTGCAGTCA GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT 
TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA 
AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG 
CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA GGGGTGTTGC AGGCTGCTCC CTCACGGGCC 
TTAGGGACCC TCCGCCAGGA CAGGGCTGAA CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC 
ATCCGGGAGG TTGACTCTTC AGAAGATGAT ACTGATGAGG GGCCTGAGAA CAGCCAAGGT 
GTTCAGGAGC CAAGCTTGGT ACCCCACCGA GAAGGGGGCC AGGATCCACC CCTCAGAGGA 
GCAGGAGAGG GTGAGGAAGA GGATGCCTTC TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA 
GTGGTCACAG GTCTACTGAC AGGGGTCACA CTGGGATCTC CCAGAATGGA AGGCCCTGGT 
GTCCCCCAGA AGAAGCTTGG AATCCTACAG GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC 
TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC ACAGACCCCA TCCCCGCTGA AGGCTGCTGG 
AAGGCCCAGC ACCTCCACAC CCAGGCACTG ACAGCACTTT GTCCGAGCTC ATCAGGACGC 
ATCCCTGCCA GTTGCTCCAC TGCCTCCACC CCTGGAGAGC TGGGCCCATG GTCCTGGAAA 
TTCCTCTTCA AGGGCCCTGA CAGGGCATAC CCAACCGGAA AGGCAGCAAT GGAAGATGGG 
CCAGCCAGCT CCCAAGATTT GGAAACTACA ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC 
AGACAGGAGG GGAAGTCATG GCCACCCAGT GCCCCTGGGC TGGCCCATCC ACCTTGTGAG 
GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA 
GCGCTGGGCA TCACCAAAGT GCCTGATGCC TCAGGTGACA GGAGGCAGGA CGTTCCATGC 
AAGAGCTGCC CCTTCACCCC AGAGCCTGGG CCCAGCCTGC TCCAGAAGGG CCAAGACCCA 
GGGGGCCCTC AGGAGCATCA GGACTTGGCA TTGTTACCTG ATGAGCTTTT AAAGCAAACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003582067.2
CDS	77..5059
Translation	MDDSSILQRRGLQKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_003582019.4
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003582019.4

ORF Insert Sequence:

ATGGATGATT CCAGTATTCT ACAAAGGAGA GGGTTACAGA AGGAGCTGAG CCTTCCAAGA 
AGAGGCAGCT TCTGTCGGAC AAGTAACCGC AAGAGTTTGA TTGTGACCTC TAGTACGTCA 
CCTACACTGC CACGGCCACA CTCACCACTC CATGGCCACA CAGGTAACAG TCCTTTGGAT 
AGTCCCCGGA ATTTCTCTCC AAATGCACCT GCTCACTTTT CCTTTGTTCC TGCCCGTAGC 
CATGGCCACA GAGCAGACAG GACTGATGGA CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT 
TCAGGCTATG GAACCAACAC CCCTAGTTCT ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG 
AAGCTGCACC AGTTGCCTTT CCAGCCCACG GCCGATGAGC TGCACTTCTT GACGAAGCAT 
TTCAGCACAG ACAGTGTGCC TGATGAGGAG GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC 
TCCCGCAGCC TCAGTCCTGG ACGGTCACCA GTTTCCTTTG ACAGTGAAAT AATAATGATG 
AATCATGTAT ACAAGGAAAG ATTCCCCAAG GCCACTGCAC AAATGGAAGA GCGTCTGGCC 
GAGTTCATCT CCTCCAATAC TCCAGACAAC GTGTTGCCCT TGGCTGATGG AACCTTGAGC 
TTTATTCATC ATCAGGTGAT AGAGATGGCC CGAGACTGCC TGGACAAATC CCGGAGTGGC 
CTCATTACCT CACACTACTT CTACGAACTT CAAGAGAATT TAGAGAAGCT TCTGCAAGAT 
GCTCATGAGC GCTCAGAGAG TTCAGAAGTG GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG 
ATGATTGTCA TTGCCCGCCC AGCTCGTCTC CTGGAATGCC TGGAGTTTGA CCCTGAAGAG 
TTCTACCACC TGTTAGAAGC AGCTGAGGGC CACGCCAAAG AGGGACAAGG GATTAAATGT 
GACATTCCCC GCTACATCGT TAGCCAACTG GGCCTCACAC GGGATCCCCT AGAGGAAATG 
GCCCAGTTGA GCAGCTATGA CAGTCCTGAT ACTCCGGAGA CAGATGATTC AGTCGAGGGC 
CGTGGGGCAT CTCTGACATC GAAAAAGACA CCCTCTGAGG AGGACTTCGA AACCATTAAG 
CTCATCAGCA ATGGCGCCTA TGGGGCCGTG TTCCTGGTGC GGCACAAGTC CACCCGGCAG 
CGCTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTGATCC TGCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGGGACAT CCTGACTTTT GCTGAGAACC CCTTTGTGGT CAGCATGTTC 
TGCTCCTTTG AAACCAAGCG CCACCTGTGC ATGGTGATGG AGTATGTGGA AGGAGGAGAC 
TGTGCCACGC TGCTGAAGAA CATCGGGGCC CTGCCTGTGG ACATGGTGCG CCTGTACTTT 
GCAGAAACTG TGCTAGCCCT GGAGTACTTA CACAATTACG GCATCGTGCA CCGTGACCTC 
AAGCCTGACA ACCTGCTGAT TACATCCATG GGTCACATCA AGCTCACTGA TTTTGGCCTT 
TCCAAAATTG GCCTTATGAG TCTGACCACA AACTTGTACG AGGGCCACAT TGAAAAGGAT 
GCACGGGAGT TCCTGGACAA GCAGGTATGC GGGACCCCGG AGTACATTGC ACCTGAGGTG 
ATCCTGCGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CTATGGGCAT CATCCTGTAC 
GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT GGAGACACTC CGGAGGAGCT CTTTGGGCAA 
GTGATCAGTG ATGAGATTGT GTGGCCTGAG GGGGACGATG CACTGCCGCC GGACGCCCAA 
GATCTCACCT CAAAACTGCT CCACCAGAAC CCATTGGAGA GACTGGGCAC AGGCAGTGCC 
TATGAGGTGA AGCAGCACCC ATTCTTCACT GGTCTGGACT GGACGGGACT TCTTCGCCAG 
AAGGCTGAAT TTATTCCTCA GCTGGAGTCA GAGGATGATA CCAGCTACTT TGACACCCGC 
TCAGAGCGAT ACCACCACGT GGACTCGGAG GATGAGGAGG AAGTGAGTGA GGATGGCTGC 
CTTGAGATTC GCCAGTTCTC GTCCTGTTCT CCAAGGTTCA GCAAGGTGTA CAGCAGCATG 
GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG ACGCCACCCC CCACCAAGCG CAGCCTGAGT 
GAGGAAAAGG AGGACCGCAC GGATGGCCTG GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG 
GTGATCGGCT CCCCTGAGAT ATTACGGAAG CGGCTGTCGG TGTCCGAGTC GTCCCACACG 
GAAAGCGATT CCAGCCCTCC ACTCACCGTG CGGCGCCACT GCTCGGGCCT CCTGGACGTG 
CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG 
GGTGCATGGC TTCTGACCCC GTCTGGAGAG GGGGCGTCTG GGCCTGCCCT GGAACGGCCG 
GCCGAGCGGC GGCTGAAGCT GAATGAGGAA CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA 
GCCATGGAGA CCCGAGGAGG CCGCGGAACC CCACAGCTGG CAGAAGGAGC CACAGCCAGG 
GCCATCAGCG ACCTGGCGGT GCGCAGGGCC CGCCACCGGC TGCTCTCTGG GGACTCCATA 
GAAAAGCGCA CCGCTCGCCC CATCAACAAA GTGATCAAGT CCGCCTCAGC CACAGCCCTG 
TCCCTTCTCA TTCCTGCTGA GCACCACTCC TGCTCTCCAC TGGCCAGCCC CATGTCCCCA 
CATTCCCAGT CATCCAACCC ATCATCCAGG GACTCTTCTC CAAGCAGGGA CTTCCTGCCA 
GCCCTCAGCA GCTCACGGCC CCCCATTGTC ATCCACCGAG CCGGCAAGAA GTATGGCTTC 
ACCCTACGAG CCATCCGTGT CTACATGGGT GACTCAGATG TCTACACCGT GCACCACATG 
GTGTGGCACG TGGAGGACGG AGGTCCAGCC AGTGAGGCAG GGCTGCGTCA GGGGGACCTC 
ATCACGCACG TCAACGGGGA GCCTGTGCAC GGGCTGGTGC ACACGGAGGT GGTGGAGCTG 
ATCCTGAAGA GTGGAAACAA GGTGTCTATT TCAACAACTC CCCTGGAGAA CACCTCCATT 
AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG 
AGCCGGGGCA AGGATGGACA AGAAAGCAGA AAAAGGAGCT CCCTGTTCCG GAAGATCACC 
AAGCAGGCAT CCCTGCTCCA CACCAGCCGC AGCCTTTCTT CCCTTAACCG CTCCCTGTCG 
TCCGGCGAGA GTGGGCCAGG CTCGCCCACA CACAGCCACA GCCTTTCTCC CCGCTCTCCC 
ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC GTGCACTCAG TGGGGGGGAA TTCATCACAG 
AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT TCTCCTGCCG GCTCTGGGCA CACACGGCCG 
AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC CAACGCCAGT ACCGCTCTCC ACGGCGCAAG 
TCAGCAGGCA GCATCCCGCT GTCACCACTG GCCCACACCC CTTCACCACC ACCAGCGGCT 
TCGCCTCAGC GTTCCCCATC ACCTCTGTCT GGCCACGGGG CCCAGGCCTT CCCTACCAAG 
CTCCACTTGT CGCCTCCCCT AGGCCGGCAA CTCTCACGGC CCAAGAGCGC GGAGCCACCC 
CGCTCACCAC TACTTAAGAG GGTGCAGTCA GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT 
TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA 
AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG 
CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA GGGGTGTTGC AGGCTGCTCC CTCACGGGCC 
TTAGGGACCC TCCGCCAGGA CAGGGCTGAA CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC 
ATCCGGGAGG TTGACTCTTC AGAAGATGAT ACTGATGAGG GGCCTGAGAA CAGCCAAGGT 
GTTCAGGAGC CAAGCTTGGT ACCCCACCGA GAAGGGGGCC AGGATCCACC CCTCAGAGGA 
GCAGGAGAGG GTGAGGAAGA GGATGCCTTC TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA 
GTGGTCACAG GTCTACTGAC AGGGGTCACA CTGGGATCTC CCAGAATGGA AGGCCCTGGT 
GTCCCCCAGA AGAAGCTTGG AATCCTACAG GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC 
TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC ACAGACCCCA TCCCCGCTGA AGGCTGCTGG 
AAGGCCCAGC ACCTCCACAC CCAGGCACTG ACAGCACTTT GTCCGAGCTC ATCAGGACGC 
ATCCCTGCCA GTTGCTCCAC TGCCTCCACC CCTGGAGAGC TGGGCCCATG GTCCTGGAAA 
TTCCTCTTCA AGGGCCCTGA CAGGGCATAC CCAACCGGAA AGGCAGCAAT GGAAGATGGG 
CCAGCCAGCT CCCAAGATTT GGAAACTACA ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC 
AGACAGGAGG GGAAGTCATG GCCACCCAGT GCCCCTGGGC TGGCCCATCC ACCTTGTGAG 
GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA 
GCGCTGGGCA TCACCAAAGT GCCTGATGCC TCAGGTGACA GGAGGCAGGA CGTTCCATGC 
AAGAGCTGCC CCTTCACCCC AGAGCCTGGG CCCAGCCTGC TCCAGAAGGG CCAAGACCCA 
GGGGGCCCTC AGGAGCATCA GGACTTGGCA TTGTTACCTG ATGAGCTTTT AAAGCAAACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_010803741.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802043.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA.

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CloneID	OBa53217
Clone ID Related Accession (Same CDS sequence)	XM_003582019.4 , XM_010803741.2 , XM_010803741.3
Accession Version	XM_010803741.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803741.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATT CCAGTATTCT ACAAAGGAGA GGGTTACAGA AGGAGCTGAG CCTTCCAAGA 
AGAGGCAGCT TCTGTCGGAC AAGTAACCGC AAGAGTTTGA TTGTGACCTC TAGTACGTCA 
CCTACACTGC CACGGCCACA CTCACCACTC CATGGCCACA CAGGTAACAG TCCTTTGGAT 
AGTCCCCGGA ATTTCTCTCC AAATGCACCT GCTCACTTTT CCTTTGTTCC TGCCCGTAGC 
CATGGCCACA GAGCAGACAG GACTGATGGA CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT 
TCAGGCTATG GAACCAACAC CCCTAGTTCT ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG 
AAGCTGCACC AGTTGCCTTT CCAGCCCACG GCCGATGAGC TGCACTTCTT GACGAAGCAT 
TTCAGCACAG ACAGTGTGCC TGATGAGGAG GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC 
TCCCGCAGCC TCAGTCCTGG ACGGTCACCA GTTTCCTTTG ACAGTGAAAT AATAATGATG 
AATCATGTAT ACAAGGAAAG ATTCCCCAAG GCCACTGCAC AAATGGAAGA GCGTCTGGCC 
GAGTTCATCT CCTCCAATAC TCCAGACAAC GTGTTGCCCT TGGCTGATGG AACCTTGAGC 
TTTATTCATC ATCAGGTGAT AGAGATGGCC CGAGACTGCC TGGACAAATC CCGGAGTGGC 
CTCATTACCT CACACTACTT CTACGAACTT CAAGAGAATT TAGAGAAGCT TCTGCAAGAT 
GCTCATGAGC GCTCAGAGAG TTCAGAAGTG GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG 
ATGATTGTCA TTGCCCGCCC AGCTCGTCTC CTGGAATGCC TGGAGTTTGA CCCTGAAGAG 
TTCTACCACC TGTTAGAAGC AGCTGAGGGC CACGCCAAAG AGGGACAAGG GATTAAATGT 
GACATTCCCC GCTACATCGT TAGCCAACTG GGCCTCACAC GGGATCCCCT AGAGGAAATG 
GCCCAGTTGA GCAGCTATGA CAGTCCTGAT ACTCCGGAGA CAGATGATTC AGTCGAGGGC 
CGTGGGGCAT CTCTGACATC GAAAAAGACA CCCTCTGAGG AGGACTTCGA AACCATTAAG 
CTCATCAGCA ATGGCGCCTA TGGGGCCGTG TTCCTGGTGC GGCACAAGTC CACCCGGCAG 
CGCTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTGATCC TGCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGGGACAT CCTGACTTTT GCTGAGAACC CCTTTGTGGT CAGCATGTTC 
TGCTCCTTTG AAACCAAGCG CCACCTGTGC ATGGTGATGG AGTATGTGGA AGGAGGAGAC 
TGTGCCACGC TGCTGAAGAA CATCGGGGCC CTGCCTGTGG ACATGGTGCG CCTGTACTTT 
GCAGAAACTG TGCTAGCCCT GGAGTACTTA CACAATTACG GCATCGTGCA CCGTGACCTC 
AAGCCTGACA ACCTGCTGAT TACATCCATG GGTCACATCA AGCTCACTGA TTTTGGCCTT 
TCCAAAATTG GCCTTATGAG TCTGACCACA AACTTGTACG AGGGCCACAT TGAAAAGGAT 
GCACGGGAGT TCCTGGACAA GCAGGTATGC GGGACCCCGG AGTACATTGC ACCTGAGGTG 
ATCCTGCGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CTATGGGCAT CATCCTGTAC 
GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT GGAGACACTC CGGAGGAGCT CTTTGGGCAA 
GTGATCAGTG ATGAGATTGT GTGGCCTGAG GGGGACGATG CACTGCCGCC GGACGCCCAA 
GATCTCACCT CAAAACTGCT CCACCAGAAC CCATTGGAGA GACTGGGCAC AGGCAGTGCC 
TATGAGGTGA AGCAGCACCC ATTCTTCACT GGTCTGGACT GGACGGGACT TCTTCGCCAG 
AAGGCTGAAT TTATTCCTCA GCTGGAGTCA GAGGATGATA CCAGCTACTT TGACACCCGC 
TCAGAGCGAT ACCACCACGT GGACTCGGAG GATGAGGAGG AAGTGAGTGA GGATGGCTGC 
CTTGAGATTC GCCAGTTCTC GTCCTGTTCT CCAAGGTTCA GCAAGGTGTA CAGCAGCATG 
GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG ACGCCACCCC CCACCAAGCG CAGCCTGAGT 
GAGGAAAAGG AGGACCGCAC GGATGGCCTG GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG 
GTGATCGGCT CCCCTGAGAT ATTACGGAAG CGGCTGTCGG TGTCCGAGTC GTCCCACACG 
GAAAGCGATT CCAGCCCTCC ACTCACCGTG CGGCGCCACT GCTCGGGCCT CCTGGACGTG 
CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG 
GGTGCATGGC TTCTGACCCC GTCTGGAGAG GGGGCGTCTG GGCCTGCCCT GGAACGGCCG 
GCCGAGCGGC GGCTGAAGCT GAATGAGGAA CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA 
GCCATGGAGA CCCGAGGAGG CCGCGGAACC CCACAGCTGG CAGAAGGAGC CACAGCCAGG 
GCCATCAGCG ACCTGGCGGT GCGCAGGGCC CGCCACCGGC TGCTCTCTGG GGACTCCATA 
GAAAAGCGCA CCGCTCGCCC CATCAACAAA GTGATCAAGT CCGCCTCAGC CACAGCCCTG 
TCCCTTCTCA TTCCTGCTGA GCACCACTCC TGCTCTCCAC TGGCCAGCCC CATGTCCCCA 
CATTCCCAGT CATCCAACCC ATCATCCAGG GACTCTTCTC CAAGCAGGGA CTTCCTGCCA 
GCCCTCAGCA GCTCACGGCC CCCCATTGTC ATCCACCGAG CCGGCAAGAA GTATGGCTTC 
ACCCTACGAG CCATCCGTGT CTACATGGGT GACTCAGATG TCTACACCGT GCACCACATG 
GTGTGGCACG TGGAGGACGG AGGTCCAGCC AGTGAGGCAG GGCTGCGTCA GGGGGACCTC 
ATCACGCACG TCAACGGGGA GCCTGTGCAC GGGCTGGTGC ACACGGAGGT GGTGGAGCTG 
ATCCTGAAGA GTGGAAACAA GGTGTCTATT TCAACAACTC CCCTGGAGAA CACCTCCATT 
AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG 
AGCCGGGGCA AGGATGGACA AGAAAGCAGA AAAAGGAGCT CCCTGTTCCG GAAGATCACC 
AAGCAGGCAT CCCTGCTCCA CACCAGCCGC AGCCTTTCTT CCCTTAACCG CTCCCTGTCG 
TCCGGCGAGA GTGGGCCAGG CTCGCCCACA CACAGCCACA GCCTTTCTCC CCGCTCTCCC 
ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC GTGCACTCAG TGGGGGGGAA TTCATCACAG 
AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT TCTCCTGCCG GCTCTGGGCA CACACGGCCG 
AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC CAACGCCAGT ACCGCTCTCC ACGGCGCAAG 
TCAGCAGGCA GCATCCCGCT GTCACCACTG GCCCACACCC CTTCACCACC ACCAGCGGCT 
TCGCCTCAGC GTTCCCCATC ACCTCTGTCT GGCCACGGGG CCCAGGCCTT CCCTACCAAG 
CTCCACTTGT CGCCTCCCCT AGGCCGGCAA CTCTCACGGC CCAAGAGCGC GGAGCCACCC 
CGCTCACCAC TACTTAAGAG GGTGCAGTCA GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT 
TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA 
AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG 
CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA GGGGTGTTGC AGGCTGCTCC CTCACGGGCC 
TTAGGGACCC TCCGCCAGGA CAGGGCTGAA CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC 
ATCCGGGAGG TTGACTCTTC AGAAGATGAT ACTGATGAGG GGCCTGAGAA CAGCCAAGGT 
GTTCAGGAGC CAAGCTTGGT ACCCCACCGA GAAGGGGGCC AGGATCCACC CCTCAGAGGA 
GCAGGAGAGG GTGAGGAAGA GGATGCCTTC TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA 
GTGGTCACAG GTCTACTGAC AGGGGTCACA CTGGGATCTC CCAGAATGGA AGGCCCTGGT 
GTCCCCCAGA AGAAGCTTGG AATCCTACAG GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC 
TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC ACAGACCCCA TCCCCGCTGA AGGCTGCTGG 
AAGGCCCAGC ACCTCCACAC CCAGGCACTG ACAGCACTTT GTCCGAGCTC ATCAGGACGC 
ATCCCTGCCA GTTGCTCCAC TGCCTCCACC CCTGGAGAGC TGGGCCCATG GTCCTGGAAA 
TTCCTCTTCA AGGGCCCTGA CAGGGCATAC CCAACCGGAA AGGCAGCAAT GGAAGATGGG 
CCAGCCAGCT CCCAAGATTT GGAAACTACA ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC 
AGACAGGAGG GGAAGTCATG GCCACCCAGT GCCCCTGGGC TGGCCCATCC ACCTTGTGAG 
GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA 
GCGCTGGGCA TCACCAAAGT GCCTGATGCC TCAGGTGACA GGAGGCAGGA CGTTCCATGC 
AAGAGCTGCC CCTTCACCCC AGAGCCTGGG CCCAGCCTGC TCCAGAAGGG CCAAGACCCA 
GGGGGCCCTC AGGAGCATCA GGACTTGGCA TTGTTACCTG ATGAGCTTTT AAAGCAAACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802043.1
CDS	77..5059
Translation	MDDSSILQRRGLQKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_010803741.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803741.2

ORF Insert Sequence:

ATGGATGATT CCAGTATTCT ACAAAGGAGA GGGTTACAGA AGGAGCTGAG CCTTCCAAGA 
AGAGGCAGCT TCTGTCGGAC AAGTAACCGC AAGAGTTTGA TTGTGACCTC TAGTACGTCA 
CCTACACTGC CACGGCCACA CTCACCACTC CATGGCCACA CAGGTAACAG TCCTTTGGAT 
AGTCCCCGGA ATTTCTCTCC AAATGCACCT GCTCACTTTT CCTTTGTTCC TGCCCGTAGC 
CATGGCCACA GAGCAGACAG GACTGATGGA CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT 
TCAGGCTATG GAACCAACAC CCCTAGTTCT ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG 
AAGCTGCACC AGTTGCCTTT CCAGCCCACG GCCGATGAGC TGCACTTCTT GACGAAGCAT 
TTCAGCACAG ACAGTGTGCC TGATGAGGAG GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC 
TCCCGCAGCC TCAGTCCTGG ACGGTCACCA GTTTCCTTTG ACAGTGAAAT AATAATGATG 
AATCATGTAT ACAAGGAAAG ATTCCCCAAG GCCACTGCAC AAATGGAAGA GCGTCTGGCC 
GAGTTCATCT CCTCCAATAC TCCAGACAAC GTGTTGCCCT TGGCTGATGG AACCTTGAGC 
TTTATTCATC ATCAGGTGAT AGAGATGGCC CGAGACTGCC TGGACAAATC CCGGAGTGGC 
CTCATTACCT CACACTACTT CTACGAACTT CAAGAGAATT TAGAGAAGCT TCTGCAAGAT 
GCTCATGAGC GCTCAGAGAG TTCAGAAGTG GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG 
ATGATTGTCA TTGCCCGCCC AGCTCGTCTC CTGGAATGCC TGGAGTTTGA CCCTGAAGAG 
TTCTACCACC TGTTAGAAGC AGCTGAGGGC CACGCCAAAG AGGGACAAGG GATTAAATGT 
GACATTCCCC GCTACATCGT TAGCCAACTG GGCCTCACAC GGGATCCCCT AGAGGAAATG 
GCCCAGTTGA GCAGCTATGA CAGTCCTGAT ACTCCGGAGA CAGATGATTC AGTCGAGGGC 
CGTGGGGCAT CTCTGACATC GAAAAAGACA CCCTCTGAGG AGGACTTCGA AACCATTAAG 
CTCATCAGCA ATGGCGCCTA TGGGGCCGTG TTCCTGGTGC GGCACAAGTC CACCCGGCAG 
CGCTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTGATCC TGCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGGGACAT CCTGACTTTT GCTGAGAACC CCTTTGTGGT CAGCATGTTC 
TGCTCCTTTG AAACCAAGCG CCACCTGTGC ATGGTGATGG AGTATGTGGA AGGAGGAGAC 
TGTGCCACGC TGCTGAAGAA CATCGGGGCC CTGCCTGTGG ACATGGTGCG CCTGTACTTT 
GCAGAAACTG TGCTAGCCCT GGAGTACTTA CACAATTACG GCATCGTGCA CCGTGACCTC 
AAGCCTGACA ACCTGCTGAT TACATCCATG GGTCACATCA AGCTCACTGA TTTTGGCCTT 
TCCAAAATTG GCCTTATGAG TCTGACCACA AACTTGTACG AGGGCCACAT TGAAAAGGAT 
GCACGGGAGT TCCTGGACAA GCAGGTATGC GGGACCCCGG AGTACATTGC ACCTGAGGTG 
ATCCTGCGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CTATGGGCAT CATCCTGTAC 
GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT GGAGACACTC CGGAGGAGCT CTTTGGGCAA 
GTGATCAGTG ATGAGATTGT GTGGCCTGAG GGGGACGATG CACTGCCGCC GGACGCCCAA 
GATCTCACCT CAAAACTGCT CCACCAGAAC CCATTGGAGA GACTGGGCAC AGGCAGTGCC 
TATGAGGTGA AGCAGCACCC ATTCTTCACT GGTCTGGACT GGACGGGACT TCTTCGCCAG 
AAGGCTGAAT TTATTCCTCA GCTGGAGTCA GAGGATGATA CCAGCTACTT TGACACCCGC 
TCAGAGCGAT ACCACCACGT GGACTCGGAG GATGAGGAGG AAGTGAGTGA GGATGGCTGC 
CTTGAGATTC GCCAGTTCTC GTCCTGTTCT CCAAGGTTCA GCAAGGTGTA CAGCAGCATG 
GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG ACGCCACCCC CCACCAAGCG CAGCCTGAGT 
GAGGAAAAGG AGGACCGCAC GGATGGCCTG GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG 
GTGATCGGCT CCCCTGAGAT ATTACGGAAG CGGCTGTCGG TGTCCGAGTC GTCCCACACG 
GAAAGCGATT CCAGCCCTCC ACTCACCGTG CGGCGCCACT GCTCGGGCCT CCTGGACGTG 
CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG 
GGTGCATGGC TTCTGACCCC GTCTGGAGAG GGGGCGTCTG GGCCTGCCCT GGAACGGCCG 
GCCGAGCGGC GGCTGAAGCT GAATGAGGAA CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA 
GCCATGGAGA CCCGAGGAGG CCGCGGAACC CCACAGCTGG CAGAAGGAGC CACAGCCAGG 
GCCATCAGCG ACCTGGCGGT GCGCAGGGCC CGCCACCGGC TGCTCTCTGG GGACTCCATA 
GAAAAGCGCA CCGCTCGCCC CATCAACAAA GTGATCAAGT CCGCCTCAGC CACAGCCCTG 
TCCCTTCTCA TTCCTGCTGA GCACCACTCC TGCTCTCCAC TGGCCAGCCC CATGTCCCCA 
CATTCCCAGT CATCCAACCC ATCATCCAGG GACTCTTCTC CAAGCAGGGA CTTCCTGCCA 
GCCCTCAGCA GCTCACGGCC CCCCATTGTC ATCCACCGAG CCGGCAAGAA GTATGGCTTC 
ACCCTACGAG CCATCCGTGT CTACATGGGT GACTCAGATG TCTACACCGT GCACCACATG 
GTGTGGCACG TGGAGGACGG AGGTCCAGCC AGTGAGGCAG GGCTGCGTCA GGGGGACCTC 
ATCACGCACG TCAACGGGGA GCCTGTGCAC GGGCTGGTGC ACACGGAGGT GGTGGAGCTG 
ATCCTGAAGA GTGGAAACAA GGTGTCTATT TCAACAACTC CCCTGGAGAA CACCTCCATT 
AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG 
AGCCGGGGCA AGGATGGACA AGAAAGCAGA AAAAGGAGCT CCCTGTTCCG GAAGATCACC 
AAGCAGGCAT CCCTGCTCCA CACCAGCCGC AGCCTTTCTT CCCTTAACCG CTCCCTGTCG 
TCCGGCGAGA GTGGGCCAGG CTCGCCCACA CACAGCCACA GCCTTTCTCC CCGCTCTCCC 
ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC GTGCACTCAG TGGGGGGGAA TTCATCACAG 
AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT TCTCCTGCCG GCTCTGGGCA CACACGGCCG 
AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC CAACGCCAGT ACCGCTCTCC ACGGCGCAAG 
TCAGCAGGCA GCATCCCGCT GTCACCACTG GCCCACACCC CTTCACCACC ACCAGCGGCT 
TCGCCTCAGC GTTCCCCATC ACCTCTGTCT GGCCACGGGG CCCAGGCCTT CCCTACCAAG 
CTCCACTTGT CGCCTCCCCT AGGCCGGCAA CTCTCACGGC CCAAGAGCGC GGAGCCACCC 
CGCTCACCAC TACTTAAGAG GGTGCAGTCA GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT 
TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA 
AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG 
CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA GGGGTGTTGC AGGCTGCTCC CTCACGGGCC 
TTAGGGACCC TCCGCCAGGA CAGGGCTGAA CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC 
ATCCGGGAGG TTGACTCTTC AGAAGATGAT ACTGATGAGG GGCCTGAGAA CAGCCAAGGT 
GTTCAGGAGC CAAGCTTGGT ACCCCACCGA GAAGGGGGCC AGGATCCACC CCTCAGAGGA 
GCAGGAGAGG GTGAGGAAGA GGATGCCTTC TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA 
GTGGTCACAG GTCTACTGAC AGGGGTCACA CTGGGATCTC CCAGAATGGA AGGCCCTGGT 
GTCCCCCAGA AGAAGCTTGG AATCCTACAG GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC 
TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC ACAGACCCCA TCCCCGCTGA AGGCTGCTGG 
AAGGCCCAGC ACCTCCACAC CCAGGCACTG ACAGCACTTT GTCCGAGCTC ATCAGGACGC 
ATCCCTGCCA GTTGCTCCAC TGCCTCCACC CCTGGAGAGC TGGGCCCATG GTCCTGGAAA 
TTCCTCTTCA AGGGCCCTGA CAGGGCATAC CCAACCGGAA AGGCAGCAAT GGAAGATGGG 
CCAGCCAGCT CCCAAGATTT GGAAACTACA ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC 
AGACAGGAGG GGAAGTCATG GCCACCCAGT GCCCCTGGGC TGGCCCATCC ACCTTGTGAG 
GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA 
GCGCTGGGCA TCACCAAAGT GCCTGATGCC TCAGGTGACA GGAGGCAGGA CGTTCCATGC 
AAGAGCTGCC CCTTCACCCC AGAGCCTGGG CCCAGCCTGC TCCAGAAGGG CCAAGACCCA 
GGGGGCCCTC AGGAGCATCA GGACTTGGCA TTGTTACCTG ATGAGCTTTT AAAGCAAACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_010803741.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802043.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA.

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CloneID	OBa53217
Clone ID Related Accession (Same CDS sequence)	XM_003582019.4 , XM_010803741.2 , XM_010803741.3
Accession Version	XM_010803741.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803741.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATT CCAGTATTCT ACAAAGGAGA GGGTTACAGA AGGAGCTGAG CCTTCCAAGA 
AGAGGCAGCT TCTGTCGGAC AAGTAACCGC AAGAGTTTGA TTGTGACCTC TAGTACGTCA 
CCTACACTGC CACGGCCACA CTCACCACTC CATGGCCACA CAGGTAACAG TCCTTTGGAT 
AGTCCCCGGA ATTTCTCTCC AAATGCACCT GCTCACTTTT CCTTTGTTCC TGCCCGTAGC 
CATGGCCACA GAGCAGACAG GACTGATGGA CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT 
TCAGGCTATG GAACCAACAC CCCTAGTTCT ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG 
AAGCTGCACC AGTTGCCTTT CCAGCCCACG GCCGATGAGC TGCACTTCTT GACGAAGCAT 
TTCAGCACAG ACAGTGTGCC TGATGAGGAG GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC 
TCCCGCAGCC TCAGTCCTGG ACGGTCACCA GTTTCCTTTG ACAGTGAAAT AATAATGATG 
AATCATGTAT ACAAGGAAAG ATTCCCCAAG GCCACTGCAC AAATGGAAGA GCGTCTGGCC 
GAGTTCATCT CCTCCAATAC TCCAGACAAC GTGTTGCCCT TGGCTGATGG AACCTTGAGC 
TTTATTCATC ATCAGGTGAT AGAGATGGCC CGAGACTGCC TGGACAAATC CCGGAGTGGC 
CTCATTACCT CACACTACTT CTACGAACTT CAAGAGAATT TAGAGAAGCT TCTGCAAGAT 
GCTCATGAGC GCTCAGAGAG TTCAGAAGTG GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG 
ATGATTGTCA TTGCCCGCCC AGCTCGTCTC CTGGAATGCC TGGAGTTTGA CCCTGAAGAG 
TTCTACCACC TGTTAGAAGC AGCTGAGGGC CACGCCAAAG AGGGACAAGG GATTAAATGT 
GACATTCCCC GCTACATCGT TAGCCAACTG GGCCTCACAC GGGATCCCCT AGAGGAAATG 
GCCCAGTTGA GCAGCTATGA CAGTCCTGAT ACTCCGGAGA CAGATGATTC AGTCGAGGGC 
CGTGGGGCAT CTCTGACATC GAAAAAGACA CCCTCTGAGG AGGACTTCGA AACCATTAAG 
CTCATCAGCA ATGGCGCCTA TGGGGCCGTG TTCCTGGTGC GGCACAAGTC CACCCGGCAG 
CGCTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTGATCC TGCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGGGACAT CCTGACTTTT GCTGAGAACC CCTTTGTGGT CAGCATGTTC 
TGCTCCTTTG AAACCAAGCG CCACCTGTGC ATGGTGATGG AGTATGTGGA AGGAGGAGAC 
TGTGCCACGC TGCTGAAGAA CATCGGGGCC CTGCCTGTGG ACATGGTGCG CCTGTACTTT 
GCAGAAACTG TGCTAGCCCT GGAGTACTTA CACAATTACG GCATCGTGCA CCGTGACCTC 
AAGCCTGACA ACCTGCTGAT TACATCCATG GGTCACATCA AGCTCACTGA TTTTGGCCTT 
TCCAAAATTG GCCTTATGAG TCTGACCACA AACTTGTACG AGGGCCACAT TGAAAAGGAT 
GCACGGGAGT TCCTGGACAA GCAGGTATGC GGGACCCCGG AGTACATTGC ACCTGAGGTG 
ATCCTGCGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CTATGGGCAT CATCCTGTAC 
GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT GGAGACACTC CGGAGGAGCT CTTTGGGCAA 
GTGATCAGTG ATGAGATTGT GTGGCCTGAG GGGGACGATG CACTGCCGCC GGACGCCCAA 
GATCTCACCT CAAAACTGCT CCACCAGAAC CCATTGGAGA GACTGGGCAC AGGCAGTGCC 
TATGAGGTGA AGCAGCACCC ATTCTTCACT GGTCTGGACT GGACGGGACT TCTTCGCCAG 
AAGGCTGAAT TTATTCCTCA GCTGGAGTCA GAGGATGATA CCAGCTACTT TGACACCCGC 
TCAGAGCGAT ACCACCACGT GGACTCGGAG GATGAGGAGG AAGTGAGTGA GGATGGCTGC 
CTTGAGATTC GCCAGTTCTC GTCCTGTTCT CCAAGGTTCA GCAAGGTGTA CAGCAGCATG 
GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG ACGCCACCCC CCACCAAGCG CAGCCTGAGT 
GAGGAAAAGG AGGACCGCAC GGATGGCCTG GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG 
GTGATCGGCT CCCCTGAGAT ATTACGGAAG CGGCTGTCGG TGTCCGAGTC GTCCCACACG 
GAAAGCGATT CCAGCCCTCC ACTCACCGTG CGGCGCCACT GCTCGGGCCT CCTGGACGTG 
CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG 
GGTGCATGGC TTCTGACCCC GTCTGGAGAG GGGGCGTCTG GGCCTGCCCT GGAACGGCCG 
GCCGAGCGGC GGCTGAAGCT GAATGAGGAA CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA 
GCCATGGAGA CCCGAGGAGG CCGCGGAACC CCACAGCTGG CAGAAGGAGC CACAGCCAGG 
GCCATCAGCG ACCTGGCGGT GCGCAGGGCC CGCCACCGGC TGCTCTCTGG GGACTCCATA 
GAAAAGCGCA CCGCTCGCCC CATCAACAAA GTGATCAAGT CCGCCTCAGC CACAGCCCTG 
TCCCTTCTCA TTCCTGCTGA GCACCACTCC TGCTCTCCAC TGGCCAGCCC CATGTCCCCA 
CATTCCCAGT CATCCAACCC ATCATCCAGG GACTCTTCTC CAAGCAGGGA CTTCCTGCCA 
GCCCTCAGCA GCTCACGGCC CCCCATTGTC ATCCACCGAG CCGGCAAGAA GTATGGCTTC 
ACCCTACGAG CCATCCGTGT CTACATGGGT GACTCAGATG TCTACACCGT GCACCACATG 
GTGTGGCACG TGGAGGACGG AGGTCCAGCC AGTGAGGCAG GGCTGCGTCA GGGGGACCTC 
ATCACGCACG TCAACGGGGA GCCTGTGCAC GGGCTGGTGC ACACGGAGGT GGTGGAGCTG 
ATCCTGAAGA GTGGAAACAA GGTGTCTATT TCAACAACTC CCCTGGAGAA CACCTCCATT 
AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG 
AGCCGGGGCA AGGATGGACA AGAAAGCAGA AAAAGGAGCT CCCTGTTCCG GAAGATCACC 
AAGCAGGCAT CCCTGCTCCA CACCAGCCGC AGCCTTTCTT CCCTTAACCG CTCCCTGTCG 
TCCGGCGAGA GTGGGCCAGG CTCGCCCACA CACAGCCACA GCCTTTCTCC CCGCTCTCCC 
ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC GTGCACTCAG TGGGGGGGAA TTCATCACAG 
AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT TCTCCTGCCG GCTCTGGGCA CACACGGCCG 
AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC CAACGCCAGT ACCGCTCTCC ACGGCGCAAG 
TCAGCAGGCA GCATCCCGCT GTCACCACTG GCCCACACCC CTTCACCACC ACCAGCGGCT 
TCGCCTCAGC GTTCCCCATC ACCTCTGTCT GGCCACGGGG CCCAGGCCTT CCCTACCAAG 
CTCCACTTGT CGCCTCCCCT AGGCCGGCAA CTCTCACGGC CCAAGAGCGC GGAGCCACCC 
CGCTCACCAC TACTTAAGAG GGTGCAGTCA GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT 
TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA 
AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG 
CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA GGGGTGTTGC AGGCTGCTCC CTCACGGGCC 
TTAGGGACCC TCCGCCAGGA CAGGGCTGAA CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC 
ATCCGGGAGG TTGACTCTTC AGAAGATGAT ACTGATGAGG GGCCTGAGAA CAGCCAAGGT 
GTTCAGGAGC CAAGCTTGGT ACCCCACCGA GAAGGGGGCC AGGATCCACC CCTCAGAGGA 
GCAGGAGAGG GTGAGGAAGA GGATGCCTTC TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA 
GTGGTCACAG GTCTACTGAC AGGGGTCACA CTGGGATCTC CCAGAATGGA AGGCCCTGGT 
GTCCCCCAGA AGAAGCTTGG AATCCTACAG GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC 
TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC ACAGACCCCA TCCCCGCTGA AGGCTGCTGG 
AAGGCCCAGC ACCTCCACAC CCAGGCACTG ACAGCACTTT GTCCGAGCTC ATCAGGACGC 
ATCCCTGCCA GTTGCTCCAC TGCCTCCACC CCTGGAGAGC TGGGCCCATG GTCCTGGAAA 
TTCCTCTTCA AGGGCCCTGA CAGGGCATAC CCAACCGGAA AGGCAGCAAT GGAAGATGGG 
CCAGCCAGCT CCCAAGATTT GGAAACTACA ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC 
AGACAGGAGG GGAAGTCATG GCCACCCAGT GCCCCTGGGC TGGCCCATCC ACCTTGTGAG 
GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA 
GCGCTGGGCA TCACCAAAGT GCCTGATGCC TCAGGTGACA GGAGGCAGGA CGTTCCATGC 
AAGAGCTGCC CCTTCACCCC AGAGCCTGGG CCCAGCCTGC TCCAGAAGGG CCAAGACCCA 
GGGGGCCCTC AGGAGCATCA GGACTTGGCA TTGTTACCTG ATGAGCTTTT AAAGCAAACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802043.1
CDS	51..5033
Translation	MDDSSILQRRGLQKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_010803741.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803741.3

ORF Insert Sequence:

ATGGATGATT CCAGTATTCT ACAAAGGAGA GGGTTACAGA AGGAGCTGAG CCTTCCAAGA 
AGAGGCAGCT TCTGTCGGAC AAGTAACCGC AAGAGTTTGA TTGTGACCTC TAGTACGTCA 
CCTACACTGC CACGGCCACA CTCACCACTC CATGGCCACA CAGGTAACAG TCCTTTGGAT 
AGTCCCCGGA ATTTCTCTCC AAATGCACCT GCTCACTTTT CCTTTGTTCC TGCCCGTAGC 
CATGGCCACA GAGCAGACAG GACTGATGGA CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT 
TCAGGCTATG GAACCAACAC CCCTAGTTCT ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG 
AAGCTGCACC AGTTGCCTTT CCAGCCCACG GCCGATGAGC TGCACTTCTT GACGAAGCAT 
TTCAGCACAG ACAGTGTGCC TGATGAGGAG GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC 
TCCCGCAGCC TCAGTCCTGG ACGGTCACCA GTTTCCTTTG ACAGTGAAAT AATAATGATG 
AATCATGTAT ACAAGGAAAG ATTCCCCAAG GCCACTGCAC AAATGGAAGA GCGTCTGGCC 
GAGTTCATCT CCTCCAATAC TCCAGACAAC GTGTTGCCCT TGGCTGATGG AACCTTGAGC 
TTTATTCATC ATCAGGTGAT AGAGATGGCC CGAGACTGCC TGGACAAATC CCGGAGTGGC 
CTCATTACCT CACACTACTT CTACGAACTT CAAGAGAATT TAGAGAAGCT TCTGCAAGAT 
GCTCATGAGC GCTCAGAGAG TTCAGAAGTG GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG 
ATGATTGTCA TTGCCCGCCC AGCTCGTCTC CTGGAATGCC TGGAGTTTGA CCCTGAAGAG 
TTCTACCACC TGTTAGAAGC AGCTGAGGGC CACGCCAAAG AGGGACAAGG GATTAAATGT 
GACATTCCCC GCTACATCGT TAGCCAACTG GGCCTCACAC GGGATCCCCT AGAGGAAATG 
GCCCAGTTGA GCAGCTATGA CAGTCCTGAT ACTCCGGAGA CAGATGATTC AGTCGAGGGC 
CGTGGGGCAT CTCTGACATC GAAAAAGACA CCCTCTGAGG AGGACTTCGA AACCATTAAG 
CTCATCAGCA ATGGCGCCTA TGGGGCCGTG TTCCTGGTGC GGCACAAGTC CACCCGGCAG 
CGCTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTGATCC TGCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGGGACAT CCTGACTTTT GCTGAGAACC CCTTTGTGGT CAGCATGTTC 
TGCTCCTTTG AAACCAAGCG CCACCTGTGC ATGGTGATGG AGTATGTGGA AGGAGGAGAC 
TGTGCCACGC TGCTGAAGAA CATCGGGGCC CTGCCTGTGG ACATGGTGCG CCTGTACTTT 
GCAGAAACTG TGCTAGCCCT GGAGTACTTA CACAATTACG GCATCGTGCA CCGTGACCTC 
AAGCCTGACA ACCTGCTGAT TACATCCATG GGTCACATCA AGCTCACTGA TTTTGGCCTT 
TCCAAAATTG GCCTTATGAG TCTGACCACA AACTTGTACG AGGGCCACAT TGAAAAGGAT 
GCACGGGAGT TCCTGGACAA GCAGGTATGC GGGACCCCGG AGTACATTGC ACCTGAGGTG 
ATCCTGCGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CTATGGGCAT CATCCTGTAC 
GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT GGAGACACTC CGGAGGAGCT CTTTGGGCAA 
GTGATCAGTG ATGAGATTGT GTGGCCTGAG GGGGACGATG CACTGCCGCC GGACGCCCAA 
GATCTCACCT CAAAACTGCT CCACCAGAAC CCATTGGAGA GACTGGGCAC AGGCAGTGCC 
TATGAGGTGA AGCAGCACCC ATTCTTCACT GGTCTGGACT GGACGGGACT TCTTCGCCAG 
AAGGCTGAAT TTATTCCTCA GCTGGAGTCA GAGGATGATA CCAGCTACTT TGACACCCGC 
TCAGAGCGAT ACCACCACGT GGACTCGGAG GATGAGGAGG AAGTGAGTGA GGATGGCTGC 
CTTGAGATTC GCCAGTTCTC GTCCTGTTCT CCAAGGTTCA GCAAGGTGTA CAGCAGCATG 
GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG ACGCCACCCC CCACCAAGCG CAGCCTGAGT 
GAGGAAAAGG AGGACCGCAC GGATGGCCTG GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG 
GTGATCGGCT CCCCTGAGAT ATTACGGAAG CGGCTGTCGG TGTCCGAGTC GTCCCACACG 
GAAAGCGATT CCAGCCCTCC ACTCACCGTG CGGCGCCACT GCTCGGGCCT CCTGGACGTG 
CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG 
GGTGCATGGC TTCTGACCCC GTCTGGAGAG GGGGCGTCTG GGCCTGCCCT GGAACGGCCG 
GCCGAGCGGC GGCTGAAGCT GAATGAGGAA CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA 
GCCATGGAGA CCCGAGGAGG CCGCGGAACC CCACAGCTGG CAGAAGGAGC CACAGCCAGG 
GCCATCAGCG ACCTGGCGGT GCGCAGGGCC CGCCACCGGC TGCTCTCTGG GGACTCCATA 
GAAAAGCGCA CCGCTCGCCC CATCAACAAA GTGATCAAGT CCGCCTCAGC CACAGCCCTG 
TCCCTTCTCA TTCCTGCTGA GCACCACTCC TGCTCTCCAC TGGCCAGCCC CATGTCCCCA 
CATTCCCAGT CATCCAACCC ATCATCCAGG GACTCTTCTC CAAGCAGGGA CTTCCTGCCA 
GCCCTCAGCA GCTCACGGCC CCCCATTGTC ATCCACCGAG CCGGCAAGAA GTATGGCTTC 
ACCCTACGAG CCATCCGTGT CTACATGGGT GACTCAGATG TCTACACCGT GCACCACATG 
GTGTGGCACG TGGAGGACGG AGGTCCAGCC AGTGAGGCAG GGCTGCGTCA GGGGGACCTC 
ATCACGCACG TCAACGGGGA GCCTGTGCAC GGGCTGGTGC ACACGGAGGT GGTGGAGCTG 
ATCCTGAAGA GTGGAAACAA GGTGTCTATT TCAACAACTC CCCTGGAGAA CACCTCCATT 
AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG 
AGCCGGGGCA AGGATGGACA AGAAAGCAGA AAAAGGAGCT CCCTGTTCCG GAAGATCACC 
AAGCAGGCAT CCCTGCTCCA CACCAGCCGC AGCCTTTCTT CCCTTAACCG CTCCCTGTCG 
TCCGGCGAGA GTGGGCCAGG CTCGCCCACA CACAGCCACA GCCTTTCTCC CCGCTCTCCC 
ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC GTGCACTCAG TGGGGGGGAA TTCATCACAG 
AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT TCTCCTGCCG GCTCTGGGCA CACACGGCCG 
AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC CAACGCCAGT ACCGCTCTCC ACGGCGCAAG 
TCAGCAGGCA GCATCCCGCT GTCACCACTG GCCCACACCC CTTCACCACC ACCAGCGGCT 
TCGCCTCAGC GTTCCCCATC ACCTCTGTCT GGCCACGGGG CCCAGGCCTT CCCTACCAAG 
CTCCACTTGT CGCCTCCCCT AGGCCGGCAA CTCTCACGGC CCAAGAGCGC GGAGCCACCC 
CGCTCACCAC TACTTAAGAG GGTGCAGTCA GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT 
TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA 
AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG 
CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA GGGGTGTTGC AGGCTGCTCC CTCACGGGCC 
TTAGGGACCC TCCGCCAGGA CAGGGCTGAA CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC 
ATCCGGGAGG TTGACTCTTC AGAAGATGAT ACTGATGAGG GGCCTGAGAA CAGCCAAGGT 
GTTCAGGAGC CAAGCTTGGT ACCCCACCGA GAAGGGGGCC AGGATCCACC CCTCAGAGGA 
GCAGGAGAGG GTGAGGAAGA GGATGCCTTC TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA 
GTGGTCACAG GTCTACTGAC AGGGGTCACA CTGGGATCTC CCAGAATGGA AGGCCCTGGT 
GTCCCCCAGA AGAAGCTTGG AATCCTACAG GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC 
TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC ACAGACCCCA TCCCCGCTGA AGGCTGCTGG 
AAGGCCCAGC ACCTCCACAC CCAGGCACTG ACAGCACTTT GTCCGAGCTC ATCAGGACGC 
ATCCCTGCCA GTTGCTCCAC TGCCTCCACC CCTGGAGAGC TGGGCCCATG GTCCTGGAAA 
TTCCTCTTCA AGGGCCCTGA CAGGGCATAC CCAACCGGAA AGGCAGCAAT GGAAGATGGG 
CCAGCCAGCT CCCAAGATTT GGAAACTACA ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC 
AGACAGGAGG GGAAGTCATG GCCACCCAGT GCCCCTGGGC TGGCCCATCC ACCTTGTGAG 
GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA 
GCGCTGGGCA TCACCAAAGT GCCTGATGCC TCAGGTGACA GGAGGCAGGA CGTTCCATGC 
AAGAGCTGCC CCTTCACCCC AGAGCCTGGG CCCAGCCTGC TCCAGAAGGG CCAAGACCCA 
GGGGGCCCTC AGGAGCATCA GGACTTGGCA TTGTTACCTG ATGAGCTTTT AAAGCAAACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_015462784.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318270.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa18158
Clone ID Related Accession (Same CDS sequence)	XM_015462784.1 , XM_005204880.2
Accession Version	XM_015462784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA 
CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT 
ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG 
GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG 
GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA 
GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG 
GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC 
GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC 
CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT 
CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG 
GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC 
CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC 
CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG 
GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT 
ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA 
CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG 
TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG 
AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT 
GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC 
ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC 
CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA 
CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG 
GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA 
AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC 
GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG 
GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT 
GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG 
GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC 
CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT 
GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA 
GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG 
GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT 
CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG 
ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG 
GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG 
CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG 
CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT 
GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG 
GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA 
CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC 
CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC 
CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA 
GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC 
TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG 
GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC 
ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT 
GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC 
AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC 
GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT 
TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC 
AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGCAGA 
AAAAGGAGCT CCCTGTTCCG GAAGATCACC AAGCAGGCAT CCCTGCTCCA CACCAGCCGC 
AGCCTTTCTT CCCTTAACCG CTCCCTGTCG TCCGGCGAGA GTGGGCCAGG CTCGCCCACA 
CACAGCCACA GCCTTTCTCC CCGCTCTCCC ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC 
GTGCACTCAG TGGGGGGGAA TTCATCACAG AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT 
TCTCCTGCCG GCTCTGGGCA CACACGGCCG AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC 
CAACGCCAGT ACCGCTCTCC ACGGCGCAAG TCAGCAGGCA GCATCCCGCT GTCACCACTG 
GCCCACACCC CTTCACCACC ACCAGCGGCT TCGCCTCAGC GTTCCCCATC ACCTCTGTCT 
GGCCACGGGG CCCAGGCCTT CCCTACCAAG CTCCACTTGT CGCCTCCCCT AGGCCGGCAA 
CTCTCACGGC CCAAGAGCGC GGAGCCACCC CGCTCACCAC TACTTAAGAG GGTGCAGTCA 
GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA 
CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC 
TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA 
GGGGTGTTGC AGGCTGCTCC CTCACGGGCC TTAGGGACCC TCCGCCAGGA CAGGGCTGAA 
CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC ATCCGGGAGG TTGACTCTTC AGAAGATGAT 
ACTGATGAGG GGCCTGAGAA CAGCCAAGGT GTTCAGGAGC CAAGCTTGGT ACCCCACCGA 
GAAGGGGGCC AGGATCCACC CCTCAGAGGA GCAGGAGAGG GTGAGGAAGA GGATGCCTTC 
TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA GTGGTCACAG GTCTACTGAC AGGGGTCACA 
CTGGGATCTC CCAGAATGGA AGGCCCTGGT GTCCCCCAGA AGAAGCTTGG AATCCTACAG 
GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC 
ACAGACCCCA TCCCCGCTGA AGGCTGCTGG AAGGCCCAGC ACCTCCACAC CCAGGCACTG 
ACAGCACTTT GTCCGAGCTC ATCAGGACGC ATCCCTGCCA GTTGCTCCAC TGCCTCCACC 
CCTGGAGAGC TGGGCCCATG GTCCTGGAAA TTCCTCTTCA AGGGCCCTGA CAGGGCATAC 
CCAACCGGAA AGGCAGCAAT GGAAGATGGG CCAGCCAGCT CCCAAGATTT GGAAACTACA 
ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC AGACAGGAGG GGAAGTCATG GCCACCCAGT 
GCCCCTGGGC TGGCCCATCC ACCTTGTGAG GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT 
GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA GCGCTGGGCA TCACCAAAGT GCCTGATGCC 
TCAGGTGACA GGAGGCAGGA CGTTCCATGC AAGAGCTGCC CCTTCACCCC AGAGCCTGGG 
CCCAGCCTGC TCCAGAAGGG CCAAGACCCA GGGGGCCCTC AGGAGCATCA GGACTTGGCA 
TTGTTACCTG ATGAGCTTTT AAAGCAAACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318270.1
CDS	299..5731
Translation	MKRSRCRDRPQPPPPPLPQTAGREDGAQRAAELPQPQPLPPRRRAPPGRQRLEERTGPVGPEVREQDVVTGRSPLLFRKLSNPDIFSSTGKAKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSGSAHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_015462784.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462784.1

ORF Insert Sequence:

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA 
CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT 
ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG 
GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG 
GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA 
GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG 
GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC 
GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC 
CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT 
CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG 
GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC 
CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC 
CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG 
GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT 
ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA 
CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG 
TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG 
AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT 
GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC 
ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC 
CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA 
CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG 
GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA 
AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC 
GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG 
GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT 
GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG 
GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC 
CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT 
GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA 
GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG 
GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT 
CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG 
ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG 
GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG 
CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG 
CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT 
GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG 
GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA 
CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC 
CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC 
CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA 
GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC 
TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG 
GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC 
ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT 
GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC 
AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC 
GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT 
TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC 
AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGCAGA 
AAAAGGAGCT CCCTGTTCCG GAAGATCACC AAGCAGGCAT CCCTGCTCCA CACCAGCCGC 
AGCCTTTCTT CCCTTAACCG CTCCCTGTCG TCCGGCGAGA GTGGGCCAGG CTCGCCCACA 
CACAGCCACA GCCTTTCTCC CCGCTCTCCC ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC 
GTGCACTCAG TGGGGGGGAA TTCATCACAG AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT 
TCTCCTGCCG GCTCTGGGCA CACACGGCCG AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC 
CAACGCCAGT ACCGCTCTCC ACGGCGCAAG TCAGCAGGCA GCATCCCGCT GTCACCACTG 
GCCCACACCC CTTCACCACC ACCAGCGGCT TCGCCTCAGC GTTCCCCATC ACCTCTGTCT 
GGCCACGGGG CCCAGGCCTT CCCTACCAAG CTCCACTTGT CGCCTCCCCT AGGCCGGCAA 
CTCTCACGGC CCAAGAGCGC GGAGCCACCC CGCTCACCAC TACTTAAGAG GGTGCAGTCA 
GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA 
CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC 
TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA 
GGGGTGTTGC AGGCTGCTCC CTCACGGGCC TTAGGGACCC TCCGCCAGGA CAGGGCTGAA 
CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC ATCCGGGAGG TTGACTCTTC AGAAGATGAT 
ACTGATGAGG GGCCTGAGAA CAGCCAAGGT GTTCAGGAGC CAAGCTTGGT ACCCCACCGA 
GAAGGGGGCC AGGATCCACC CCTCAGAGGA GCAGGAGAGG GTGAGGAAGA GGATGCCTTC 
TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA GTGGTCACAG GTCTACTGAC AGGGGTCACA 
CTGGGATCTC CCAGAATGGA AGGCCCTGGT GTCCCCCAGA AGAAGCTTGG AATCCTACAG 
GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC 
ACAGACCCCA TCCCCGCTGA AGGCTGCTGG AAGGCCCAGC ACCTCCACAC CCAGGCACTG 
ACAGCACTTT GTCCGAGCTC ATCAGGACGC ATCCCTGCCA GTTGCTCCAC TGCCTCCACC 
CCTGGAGAGC TGGGCCCATG GTCCTGGAAA TTCCTCTTCA AGGGCCCTGA CAGGGCATAC 
CCAACCGGAA AGGCAGCAAT GGAAGATGGG CCAGCCAGCT CCCAAGATTT GGAAACTACA 
ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC AGACAGGAGG GGAAGTCATG GCCACCCAGT 
GCCCCTGGGC TGGCCCATCC ACCTTGTGAG GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT 
GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA GCGCTGGGCA TCACCAAAGT GCCTGATGCC 
TCAGGTGACA GGAGGCAGGA CGTTCCATGC AAGAGCTGCC CCTTCACCCC AGAGCCTGGG 
CCCAGCCTGC TCCAGAAGGG CCAAGACCCA GGGGGCCCTC AGGAGCATCA GGACTTGGCA 
TTGTTACCTG ATGAGCTTTT AAAGCAAACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_005204880.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204937.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.

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CloneID	OBa18158
Clone ID Related Accession (Same CDS sequence)	XM_015462784.1 , XM_005204880.2
Accession Version	XM_005204880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005204880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA 
CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT 
ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG 
GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG 
GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA 
GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG 
GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC 
GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC 
CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT 
CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG 
GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC 
CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC 
CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG 
GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT 
ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA 
CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG 
TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG 
AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT 
GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC 
ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC 
CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA 
CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG 
GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA 
AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC 
GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG 
GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT 
GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG 
GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC 
CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT 
GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA 
GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG 
GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT 
CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG 
ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG 
GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG 
CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG 
CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT 
GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG 
GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA 
CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC 
CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC 
CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA 
GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC 
TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG 
GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC 
ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT 
GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC 
AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC 
GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT 
TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC 
AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGCAGA 
AAAAGGAGCT CCCTGTTCCG GAAGATCACC AAGCAGGCAT CCCTGCTCCA CACCAGCCGC 
AGCCTTTCTT CCCTTAACCG CTCCCTGTCG TCCGGCGAGA GTGGGCCAGG CTCGCCCACA 
CACAGCCACA GCCTTTCTCC CCGCTCTCCC ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC 
GTGCACTCAG TGGGGGGGAA TTCATCACAG AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT 
TCTCCTGCCG GCTCTGGGCA CACACGGCCG AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC 
CAACGCCAGT ACCGCTCTCC ACGGCGCAAG TCAGCAGGCA GCATCCCGCT GTCACCACTG 
GCCCACACCC CTTCACCACC ACCAGCGGCT TCGCCTCAGC GTTCCCCATC ACCTCTGTCT 
GGCCACGGGG CCCAGGCCTT CCCTACCAAG CTCCACTTGT CGCCTCCCCT AGGCCGGCAA 
CTCTCACGGC CCAAGAGCGC GGAGCCACCC CGCTCACCAC TACTTAAGAG GGTGCAGTCA 
GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA 
CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC 
TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA 
GGGGTGTTGC AGGCTGCTCC CTCACGGGCC TTAGGGACCC TCCGCCAGGA CAGGGCTGAA 
CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC ATCCGGGAGG TTGACTCTTC AGAAGATGAT 
ACTGATGAGG GGCCTGAGAA CAGCCAAGGT GTTCAGGAGC CAAGCTTGGT ACCCCACCGA 
GAAGGGGGCC AGGATCCACC CCTCAGAGGA GCAGGAGAGG GTGAGGAAGA GGATGCCTTC 
TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA GTGGTCACAG GTCTACTGAC AGGGGTCACA 
CTGGGATCTC CCAGAATGGA AGGCCCTGGT GTCCCCCAGA AGAAGCTTGG AATCCTACAG 
GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC 
ACAGACCCCA TCCCCGCTGA AGGCTGCTGG AAGGCCCAGC ACCTCCACAC CCAGGCACTG 
ACAGCACTTT GTCCGAGCTC ATCAGGACGC ATCCCTGCCA GTTGCTCCAC TGCCTCCACC 
CCTGGAGAGC TGGGCCCATG GTCCTGGAAA TTCCTCTTCA AGGGCCCTGA CAGGGCATAC 
CCAACCGGAA AGGCAGCAAT GGAAGATGGG CCAGCCAGCT CCCAAGATTT GGAAACTACA 
ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC AGACAGGAGG GGAAGTCATG GCCACCCAGT 
GCCCCTGGGC TGGCCCATCC ACCTTGTGAG GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT 
GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA GCGCTGGGCA TCACCAAAGT GCCTGATGCC 
TCAGGTGACA GGAGGCAGGA CGTTCCATGC AAGAGCTGCC CCTTCACCCC AGAGCCTGGG 
CCCAGCCTGC TCCAGAAGGG CCAAGACCCA GGGGGCCCTC AGGAGCATCA GGACTTGGCA 
TTGTTACCTG ATGAGCTTTT AAAGCAAACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204937.1
CDS	299..5731
Translation	MKRSRCRDRPQPPPPPLPQTAGREDGAQRAAELPQPQPLPPRRRAPPGRQRLEERTGPVGPEVREQDVVTGRSPLLFRKLSNPDIFSSTGKAKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSGSAHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_005204880.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204880.2

ORF Insert Sequence:

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA 
CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT 
ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG 
GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG 
GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA 
GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG 
GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC 
GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC 
CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT 
CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG 
GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC 
CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC 
CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG 
GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT 
ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA 
CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG 
TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG 
AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT 
GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC 
ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC 
CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA 
CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG 
GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA 
AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC 
GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG 
GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT 
GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG 
GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC 
CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT 
GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA 
GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG 
GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT 
CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG 
ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG 
GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG 
CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG 
CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT 
GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG 
GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA 
CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC 
CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC 
CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA 
GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC 
TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG 
GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC 
ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT 
GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC 
AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC 
GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT 
TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC 
AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGCAGA 
AAAAGGAGCT CCCTGTTCCG GAAGATCACC AAGCAGGCAT CCCTGCTCCA CACCAGCCGC 
AGCCTTTCTT CCCTTAACCG CTCCCTGTCG TCCGGCGAGA GTGGGCCAGG CTCGCCCACA 
CACAGCCACA GCCTTTCTCC CCGCTCTCCC ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC 
GTGCACTCAG TGGGGGGGAA TTCATCACAG AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT 
TCTCCTGCCG GCTCTGGGCA CACACGGCCG AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC 
CAACGCCAGT ACCGCTCTCC ACGGCGCAAG TCAGCAGGCA GCATCCCGCT GTCACCACTG 
GCCCACACCC CTTCACCACC ACCAGCGGCT TCGCCTCAGC GTTCCCCATC ACCTCTGTCT 
GGCCACGGGG CCCAGGCCTT CCCTACCAAG CTCCACTTGT CGCCTCCCCT AGGCCGGCAA 
CTCTCACGGC CCAAGAGCGC GGAGCCACCC CGCTCACCAC TACTTAAGAG GGTGCAGTCA 
GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA 
CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC 
TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA 
GGGGTGTTGC AGGCTGCTCC CTCACGGGCC TTAGGGACCC TCCGCCAGGA CAGGGCTGAA 
CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC ATCCGGGAGG TTGACTCTTC AGAAGATGAT 
ACTGATGAGG GGCCTGAGAA CAGCCAAGGT GTTCAGGAGC CAAGCTTGGT ACCCCACCGA 
GAAGGGGGCC AGGATCCACC CCTCAGAGGA GCAGGAGAGG GTGAGGAAGA GGATGCCTTC 
TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA GTGGTCACAG GTCTACTGAC AGGGGTCACA 
CTGGGATCTC CCAGAATGGA AGGCCCTGGT GTCCCCCAGA AGAAGCTTGG AATCCTACAG 
GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC 
ACAGACCCCA TCCCCGCTGA AGGCTGCTGG AAGGCCCAGC ACCTCCACAC CCAGGCACTG 
ACAGCACTTT GTCCGAGCTC ATCAGGACGC ATCCCTGCCA GTTGCTCCAC TGCCTCCACC 
CCTGGAGAGC TGGGCCCATG GTCCTGGAAA TTCCTCTTCA AGGGCCCTGA CAGGGCATAC 
CCAACCGGAA AGGCAGCAAT GGAAGATGGG CCAGCCAGCT CCCAAGATTT GGAAACTACA 
ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC AGACAGGAGG GGAAGTCATG GCCACCCAGT 
GCCCCTGGGC TGGCCCATCC ACCTTGTGAG GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT 
GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA GCGCTGGGCA TCACCAAAGT GCCTGATGCC 
TCAGGTGACA GGAGGCAGGA CGTTCCATGC AAGAGCTGCC CCTTCACCCC AGAGCCTGGG 
CCCAGCCTGC TCCAGAAGGG CCAAGACCCA GGGGGCCCTC AGGAGCATCA GGACTTGGCA 
TTGTTACCTG ATGAGCTTTT AAAGCAAACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_005204881.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204938.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.

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CloneID	OBa18159
Clone ID Related Accession (Same CDS sequence)	XM_005204881.2 , XM_015462785.1
Accession Version	XM_005204881.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005204881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA 
CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT 
ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG 
GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG 
GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA 
GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG 
GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC 
GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC 
CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT 
CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG 
GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC 
CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC 
CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG 
GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT 
ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA 
CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG 
TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG 
AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT 
GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC 
ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC 
CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA 
CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG 
GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA 
AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC 
GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG 
GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT 
GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG 
GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC 
CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT 
GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA 
GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG 
GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT 
CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG 
ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG 
GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG 
CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG 
CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT 
GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG 
GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA 
CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC 
CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC 
CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA 
GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC 
TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG 
GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC 
ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT 
GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC 
AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC 
GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT 
TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC 
AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGAAAA 
AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC 
CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC 
AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG 
CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT 
CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA 
CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC 
CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC 
CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC 
TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT 
GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG 
AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT 
CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG 
GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG 
CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT 
GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA 
GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG 
CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG 
GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT 
TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA 
GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA 
GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT 
GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA 
ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT 
CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC 
CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG 
TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA 
GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC 
AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG 
TTACCTGATG AGCTTTTAAA GCAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204938.1
CDS	299..5728
Translation	MKRSRCRDRPQPPPPPLPQTAGREDGAQRAAELPQPQPLPPRRRAPPGRQRLEERTGPVGPEVREQDVVTGRSPLLFRKLSNPDIFSSTGKAKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSGSAHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQERKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_005204881.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204881.2

ORF Insert Sequence:

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA 
CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT 
ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG 
GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG 
GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA 
GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG 
GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC 
GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC 
CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT 
CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG 
GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC 
CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC 
CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG 
GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT 
ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA 
CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG 
TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG 
AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT 
GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC 
ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC 
CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA 
CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG 
GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA 
AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC 
GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG 
GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT 
GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG 
GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC 
CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT 
GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA 
GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG 
GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT 
CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG 
ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG 
GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG 
CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG 
CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT 
GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG 
GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA 
CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC 
CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC 
CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA 
GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC 
TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG 
GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC 
ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT 
GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC 
AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC 
GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT 
TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC 
AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGAAAA 
AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC 
CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC 
AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG 
CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT 
CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA 
CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC 
CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC 
CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC 
TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT 
GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG 
AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT 
CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG 
GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG 
CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT 
GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA 
GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG 
CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG 
GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT 
TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA 
GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA 
GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT 
GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA 
ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT 
CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC 
CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG 
TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA 
GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC 
AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG 
TTACCTGATG AGCTTTTAAA GCAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_015462785.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318271.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.

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CloneID	OBa18159
Clone ID Related Accession (Same CDS sequence)	XM_005204881.2 , XM_015462785.1
Accession Version	XM_015462785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA 
CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT 
ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG 
GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG 
GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA 
GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG 
GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC 
GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC 
CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT 
CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG 
GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC 
CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC 
CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG 
GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT 
ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA 
CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG 
TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG 
AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT 
GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC 
ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC 
CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA 
CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG 
GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA 
AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC 
GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG 
GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT 
GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG 
GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC 
CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT 
GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA 
GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG 
GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT 
CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG 
ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG 
GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG 
CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG 
CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT 
GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG 
GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA 
CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC 
CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC 
CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA 
GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC 
TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG 
GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC 
ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT 
GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC 
AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC 
GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT 
TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC 
AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGAAAA 
AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC 
CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC 
AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG 
CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT 
CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA 
CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC 
CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC 
CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC 
TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT 
GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG 
AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT 
CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG 
GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG 
CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT 
GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA 
GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG 
CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG 
GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT 
TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA 
GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA 
GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT 
GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA 
ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT 
CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC 
CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG 
TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA 
GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC 
AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG 
TTACCTGATG AGCTTTTAAA GCAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318271.1
CDS	299..5728
Translation	MKRSRCRDRPQPPPPPLPQTAGREDGAQRAAELPQPQPLPPRRRAPPGRQRLEERTGPVGPEVREQDVVTGRSPLLFRKLSNPDIFSSTGKAKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSGSAHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQERKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_015462785.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462785.1

ORF Insert Sequence:

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA 
CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT 
ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG 
GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG 
GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA 
GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG 
GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC 
GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC 
CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT 
CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG 
GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC 
CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC 
CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG 
GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT 
ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA 
CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG 
TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG 
AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT 
GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC 
ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC 
CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA 
CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG 
GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA 
AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC 
GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG 
GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT 
GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG 
GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC 
CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT 
GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA 
GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG 
GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT 
CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG 
ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG 
GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG 
CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG 
CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT 
GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG 
GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA 
CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC 
CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC 
CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA 
GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC 
TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG 
GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC 
ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT 
GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC 
AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC 
GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT 
TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC 
AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGAAAA 
AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC 
CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC 
AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG 
CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT 
CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA 
CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC 
CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC 
CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC 
TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT 
GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG 
AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT 
CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG 
GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG 
CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT 
GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA 
GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG 
CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG 
GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT 
TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA 
GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA 
GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT 
GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA 
ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT 
CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC 
CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG 
TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA 
GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC 
AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG 
TTACCTGATG AGCTTTTAAA GCAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_003585907.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003585955.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.

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CloneID	OBa18160
Clone ID Related Accession (Same CDS sequence)	XM_003585907.3 , XM_015462786.1
Accession Version	XM_003585907.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_003585907.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA 
TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC 
TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG 
ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG 
CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA 
ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG 
CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA 
ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC 
CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT 
CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG 
AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC 
CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG 
ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA 
GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA 
GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA 
ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC 
ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC 
AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA 
GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC 
CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC 
CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT 
TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT 
GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA 
CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC 
ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC 
TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG 
GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA 
GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT 
CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT 
GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG 
GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC 
AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC 
AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG 
CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG 
TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC 
CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG 
CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG 
GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC 
CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC 
ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG 
GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC 
ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC 
ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC 
TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG 
TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG 
CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG 
GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG 
GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC 
ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG 
AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG 
AAGATCACCA AGCAGGCATC CCTGCTCCAC ACCAGCCGCA GCCTTTCTTC CCTTAACCGC 
TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC TCGCCCACAC ACAGCCACAG CCTTTCTCCC 
CGCTCTCCCA CGCAGGGCTA CCGGGTGACC CCTGAAGCCG TGCACTCAGT GGGGGGGAAT 
TCATCACAGA GCAGCTCCCC CAGCTCCAGC GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC 
ACACGGCCGA GCTCCCTCCA CGGTCTGGCG CCCAAGCTCC AACGCCAGTA CCGCTCTCCA 
CGGCGCAAGT CAGCAGGCAG CATCCCGCTG TCACCACTGG CCCACACCCC TTCACCACCA 
CCAGCGGCTT CGCCTCAGCG TTCCCCATCA CCTCTGTCTG GCCACGGGGC CCAGGCCTTC 
CCTACCAAGC TCCACTTGTC GCCTCCCCTA GGCCGGCAAC TCTCACGGCC CAAGAGCGCG 
GAGCCACCCC GCTCACCACT ACTTAAGAGG GTGCAGTCAG CTGAAAAACT GGCAGCTGCA 
CTGGCTGCTT CTGAGAAGAA GCTCGCCACT TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC 
CCCGAGCTAA AGAAGGAATT GCCGCCCAGG GAAGTCAGCT CTCTGGAGGT AGTTGGGACC 
AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC 
TCACGGGCCT TAGGGACCCT CCGCCAGGAC AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG 
CAGGAAGCCA TCCGGGAGGT TGACTCTTCA GAAGATGATA CTGATGAGGG GCCTGAGAAC 
AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA CCCCACCGAG AAGGGGGCCA GGATCCACCC 
CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG GATGCCTTCT TGCCCAGGGA TCCCAGGGGA 
CAGGGCCCAG TGGTCACAGG TCTACTGACA GGGGTCACAC TGGGATCTCC CAGAATGGAA 
GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA ATCCTACAGG CTTTTGAAGA GGGTTCCAGC 
TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG GCTGGCCCCA CAGACCCCAT CCCCGCTGAA 
GGCTGCTGGA AGGCCCAGCA CCTCCACACC CAGGCACTGA CAGCACTTTG TCCGAGCTCA 
TCAGGACGCA TCCCTGCCAG TTGCTCCACT GCCTCCACCC CTGGAGAGCT GGGCCCATGG 
TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC AGGGCATACC CAACCGGAAA GGCAGCAATG 
GAAGATGGGC CAGCCAGCTC CCAAGATTTG GAAACTACAA CTCCAGGCCA CCCTGCAAAC 
CTGTCCCCCA GACAGGAGGG GAAGTCATGG CCACCCAGTG CCCCTGGGCT GGCCCATCCA 
CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA TGGGAGTCTG AGTGTGCACA GAGAGAGAAA 
GAGGAGCCAG CGCTGGGCAT CACCAAAGTG CCTGATGCCT CAGGTGACAG GAGGCAGGAC 
GTTCCATGCA AGAGCTGCCC CTTCACCCCA GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC 
CAAGACCCAG GGGGCCCTCA GGAGCATCAG GACTTGGCAT TGTTACCTGA TGAGCTTTTA 
AAGCAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003585955.1
CDS	299..5710
Translation	MKRSRCRDRPQPPPPPLPQTAGREDGAQRAAELPQPQPLPPRRRAPPGRQRLEERTGPVGPEVREQDVVTGRSPLLFRKLSNPDIFSSTGKAKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSGSAHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_003585907.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003585907.3

ORF Insert Sequence:

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA 
TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC 
TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG 
ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG 
CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA 
ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG 
CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA 
ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC 
CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT 
CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG 
AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC 
CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG 
ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA 
GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA 
GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA 
ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC 
ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC 
AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA 
GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC 
CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC 
CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT 
TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT 
GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA 
CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC 
ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC 
TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG 
GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA 
GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT 
CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT 
GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG 
GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC 
AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC 
AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG 
CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG 
TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC 
CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG 
CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG 
GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC 
CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC 
ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG 
GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC 
ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC 
ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC 
TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG 
TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG 
CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG 
GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG 
GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC 
ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG 
AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG 
AAGATCACCA AGCAGGCATC CCTGCTCCAC ACCAGCCGCA GCCTTTCTTC CCTTAACCGC 
TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC TCGCCCACAC ACAGCCACAG CCTTTCTCCC 
CGCTCTCCCA CGCAGGGCTA CCGGGTGACC CCTGAAGCCG TGCACTCAGT GGGGGGGAAT 
TCATCACAGA GCAGCTCCCC CAGCTCCAGC GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC 
ACACGGCCGA GCTCCCTCCA CGGTCTGGCG CCCAAGCTCC AACGCCAGTA CCGCTCTCCA 
CGGCGCAAGT CAGCAGGCAG CATCCCGCTG TCACCACTGG CCCACACCCC TTCACCACCA 
CCAGCGGCTT CGCCTCAGCG TTCCCCATCA CCTCTGTCTG GCCACGGGGC CCAGGCCTTC 
CCTACCAAGC TCCACTTGTC GCCTCCCCTA GGCCGGCAAC TCTCACGGCC CAAGAGCGCG 
GAGCCACCCC GCTCACCACT ACTTAAGAGG GTGCAGTCAG CTGAAAAACT GGCAGCTGCA 
CTGGCTGCTT CTGAGAAGAA GCTCGCCACT TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC 
CCCGAGCTAA AGAAGGAATT GCCGCCCAGG GAAGTCAGCT CTCTGGAGGT AGTTGGGACC 
AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC 
TCACGGGCCT TAGGGACCCT CCGCCAGGAC AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG 
CAGGAAGCCA TCCGGGAGGT TGACTCTTCA GAAGATGATA CTGATGAGGG GCCTGAGAAC 
AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA CCCCACCGAG AAGGGGGCCA GGATCCACCC 
CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG GATGCCTTCT TGCCCAGGGA TCCCAGGGGA 
CAGGGCCCAG TGGTCACAGG TCTACTGACA GGGGTCACAC TGGGATCTCC CAGAATGGAA 
GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA ATCCTACAGG CTTTTGAAGA GGGTTCCAGC 
TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG GCTGGCCCCA CAGACCCCAT CCCCGCTGAA 
GGCTGCTGGA AGGCCCAGCA CCTCCACACC CAGGCACTGA CAGCACTTTG TCCGAGCTCA 
TCAGGACGCA TCCCTGCCAG TTGCTCCACT GCCTCCACCC CTGGAGAGCT GGGCCCATGG 
TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC AGGGCATACC CAACCGGAAA GGCAGCAATG 
GAAGATGGGC CAGCCAGCTC CCAAGATTTG GAAACTACAA CTCCAGGCCA CCCTGCAAAC 
CTGTCCCCCA GACAGGAGGG GAAGTCATGG CCACCCAGTG CCCCTGGGCT GGCCCATCCA 
CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA TGGGAGTCTG AGTGTGCACA GAGAGAGAAA 
GAGGAGCCAG CGCTGGGCAT CACCAAAGTG CCTGATGCCT CAGGTGACAG GAGGCAGGAC 
GTTCCATGCA AGAGCTGCCC CTTCACCCCA GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC 
CAAGACCCAG GGGGCCCTCA GGAGCATCAG GACTTGGCAT TGTTACCTGA TGAGCTTTTA 
AAGCAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_015462786.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318272.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.

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CloneID	OBa18160
Clone ID Related Accession (Same CDS sequence)	XM_003585907.3 , XM_015462786.1
Accession Version	XM_015462786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA 
TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC 
TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG 
ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG 
CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA 
ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG 
CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA 
ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC 
CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT 
CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG 
AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC 
CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG 
ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA 
GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA 
GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA 
ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC 
ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC 
AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA 
GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC 
CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC 
CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT 
TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT 
GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA 
CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC 
ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC 
TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG 
GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA 
GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT 
CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT 
GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG 
GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC 
AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC 
AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG 
CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG 
TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC 
CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG 
CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG 
GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC 
CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC 
ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG 
GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC 
ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC 
ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC 
TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG 
TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG 
CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG 
GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG 
GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC 
ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG 
AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG 
AAGATCACCA AGCAGGCATC CCTGCTCCAC ACCAGCCGCA GCCTTTCTTC CCTTAACCGC 
TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC TCGCCCACAC ACAGCCACAG CCTTTCTCCC 
CGCTCTCCCA CGCAGGGCTA CCGGGTGACC CCTGAAGCCG TGCACTCAGT GGGGGGGAAT 
TCATCACAGA GCAGCTCCCC CAGCTCCAGC GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC 
ACACGGCCGA GCTCCCTCCA CGGTCTGGCG CCCAAGCTCC AACGCCAGTA CCGCTCTCCA 
CGGCGCAAGT CAGCAGGCAG CATCCCGCTG TCACCACTGG CCCACACCCC TTCACCACCA 
CCAGCGGCTT CGCCTCAGCG TTCCCCATCA CCTCTGTCTG GCCACGGGGC CCAGGCCTTC 
CCTACCAAGC TCCACTTGTC GCCTCCCCTA GGCCGGCAAC TCTCACGGCC CAAGAGCGCG 
GAGCCACCCC GCTCACCACT ACTTAAGAGG GTGCAGTCAG CTGAAAAACT GGCAGCTGCA 
CTGGCTGCTT CTGAGAAGAA GCTCGCCACT TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC 
CCCGAGCTAA AGAAGGAATT GCCGCCCAGG GAAGTCAGCT CTCTGGAGGT AGTTGGGACC 
AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC 
TCACGGGCCT TAGGGACCCT CCGCCAGGAC AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG 
CAGGAAGCCA TCCGGGAGGT TGACTCTTCA GAAGATGATA CTGATGAGGG GCCTGAGAAC 
AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA CCCCACCGAG AAGGGGGCCA GGATCCACCC 
CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG GATGCCTTCT TGCCCAGGGA TCCCAGGGGA 
CAGGGCCCAG TGGTCACAGG TCTACTGACA GGGGTCACAC TGGGATCTCC CAGAATGGAA 
GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA ATCCTACAGG CTTTTGAAGA GGGTTCCAGC 
TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG GCTGGCCCCA CAGACCCCAT CCCCGCTGAA 
GGCTGCTGGA AGGCCCAGCA CCTCCACACC CAGGCACTGA CAGCACTTTG TCCGAGCTCA 
TCAGGACGCA TCCCTGCCAG TTGCTCCACT GCCTCCACCC CTGGAGAGCT GGGCCCATGG 
TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC AGGGCATACC CAACCGGAAA GGCAGCAATG 
GAAGATGGGC CAGCCAGCTC CCAAGATTTG GAAACTACAA CTCCAGGCCA CCCTGCAAAC 
CTGTCCCCCA GACAGGAGGG GAAGTCATGG CCACCCAGTG CCCCTGGGCT GGCCCATCCA 
CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA TGGGAGTCTG AGTGTGCACA GAGAGAGAAA 
GAGGAGCCAG CGCTGGGCAT CACCAAAGTG CCTGATGCCT CAGGTGACAG GAGGCAGGAC 
GTTCCATGCA AGAGCTGCCC CTTCACCCCA GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC 
CAAGACCCAG GGGGCCCTCA GGAGCATCAG GACTTGGCAT TGTTACCTGA TGAGCTTTTA 
AAGCAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318272.1
CDS	299..5710
Translation	MKRSRCRDRPQPPPPPLPQTAGREDGAQRAAELPQPQPLPPRRRAPPGRQRLEERTGPVGPEVREQDVVTGRSPLLFRKLSNPDIFSSTGKAKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSGSAHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_015462786.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462786.1

ORF Insert Sequence:

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA 
TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC 
TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG 
ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG 
CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA 
ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG 
CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA 
ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC 
CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT 
CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG 
AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC 
CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG 
ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA 
GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA 
GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA 
ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC 
ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC 
AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA 
GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC 
CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC 
CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT 
TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT 
GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA 
CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC 
ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC 
TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG 
GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA 
GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT 
CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT 
GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG 
GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC 
AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC 
AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG 
CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG 
TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC 
CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG 
CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG 
GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC 
CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC 
ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG 
GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC 
ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC 
ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC 
TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG 
TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG 
CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG 
GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG 
GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC 
ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG 
AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG 
AAGATCACCA AGCAGGCATC CCTGCTCCAC ACCAGCCGCA GCCTTTCTTC CCTTAACCGC 
TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC TCGCCCACAC ACAGCCACAG CCTTTCTCCC 
CGCTCTCCCA CGCAGGGCTA CCGGGTGACC CCTGAAGCCG TGCACTCAGT GGGGGGGAAT 
TCATCACAGA GCAGCTCCCC CAGCTCCAGC GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC 
ACACGGCCGA GCTCCCTCCA CGGTCTGGCG CCCAAGCTCC AACGCCAGTA CCGCTCTCCA 
CGGCGCAAGT CAGCAGGCAG CATCCCGCTG TCACCACTGG CCCACACCCC TTCACCACCA 
CCAGCGGCTT CGCCTCAGCG TTCCCCATCA CCTCTGTCTG GCCACGGGGC CCAGGCCTTC 
CCTACCAAGC TCCACTTGTC GCCTCCCCTA GGCCGGCAAC TCTCACGGCC CAAGAGCGCG 
GAGCCACCCC GCTCACCACT ACTTAAGAGG GTGCAGTCAG CTGAAAAACT GGCAGCTGCA 
CTGGCTGCTT CTGAGAAGAA GCTCGCCACT TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC 
CCCGAGCTAA AGAAGGAATT GCCGCCCAGG GAAGTCAGCT CTCTGGAGGT AGTTGGGACC 
AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC 
TCACGGGCCT TAGGGACCCT CCGCCAGGAC AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG 
CAGGAAGCCA TCCGGGAGGT TGACTCTTCA GAAGATGATA CTGATGAGGG GCCTGAGAAC 
AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA CCCCACCGAG AAGGGGGCCA GGATCCACCC 
CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG GATGCCTTCT TGCCCAGGGA TCCCAGGGGA 
CAGGGCCCAG TGGTCACAGG TCTACTGACA GGGGTCACAC TGGGATCTCC CAGAATGGAA 
GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA ATCCTACAGG CTTTTGAAGA GGGTTCCAGC 
TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG GCTGGCCCCA CAGACCCCAT CCCCGCTGAA 
GGCTGCTGGA AGGCCCAGCA CCTCCACACC CAGGCACTGA CAGCACTTTG TCCGAGCTCA 
TCAGGACGCA TCCCTGCCAG TTGCTCCACT GCCTCCACCC CTGGAGAGCT GGGCCCATGG 
TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC AGGGCATACC CAACCGGAAA GGCAGCAATG 
GAAGATGGGC CAGCCAGCTC CCAAGATTTG GAAACTACAA CTCCAGGCCA CCCTGCAAAC 
CTGTCCCCCA GACAGGAGGG GAAGTCATGG CCACCCAGTG CCCCTGGGCT GGCCCATCCA 
CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA TGGGAGTCTG AGTGTGCACA GAGAGAGAAA 
GAGGAGCCAG CGCTGGGCAT CACCAAAGTG CCTGATGCCT CAGGTGACAG GAGGCAGGAC 
GTTCCATGCA AGAGCTGCCC CTTCACCCCA GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC 
CAAGACCCAG GGGGCCCTCA GGAGCATCAG GACTTGGCAT TGTTACCTGA TGAGCTTTTA 
AAGCAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_015462787.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318273.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.

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CloneID	OBa18161
Clone ID Related Accession (Same CDS sequence)	XM_015462787.1 , XM_005204882.2
Accession Version	XM_015462787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA 
TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC 
TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG 
ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG 
CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA 
ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG 
CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA 
ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC 
CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT 
CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG 
AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC 
CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG 
ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA 
GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA 
GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA 
ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC 
ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC 
AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA 
GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC 
CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC 
CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT 
TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT 
GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA 
CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC 
ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC 
TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG 
GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA 
GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT 
CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT 
GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG 
GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC 
AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC 
AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG 
CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG 
TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC 
CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG 
CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG 
GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC 
CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC 
ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG 
GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC 
ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC 
ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC 
TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG 
TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG 
CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG 
GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG 
GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC 
ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG 
AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGAAAAA GGAGCTCCCT GTTCCGGAAG 
ATCACCAAGC AGGCATCCCT GCTCCACACC AGCCGCAGCC TTTCTTCCCT TAACCGCTCC 
CTGTCGTCCG GCGAGAGTGG GCCAGGCTCG CCCACACACA GCCACAGCCT TTCTCCCCGC 
TCTCCCACGC AGGGCTACCG GGTGACCCCT GAAGCCGTGC ACTCAGTGGG GGGGAATTCA 
TCACAGAGCA GCTCCCCCAG CTCCAGCGTG CCCAGTTCTC CTGCCGGCTC TGGGCACACA 
CGGCCGAGCT CCCTCCACGG TCTGGCGCCC AAGCTCCAAC GCCAGTACCG CTCTCCACGG 
CGCAAGTCAG CAGGCAGCAT CCCGCTGTCA CCACTGGCCC ACACCCCTTC ACCACCACCA 
GCGGCTTCGC CTCAGCGTTC CCCATCACCT CTGTCTGGCC ACGGGGCCCA GGCCTTCCCT 
ACCAAGCTCC ACTTGTCGCC TCCCCTAGGC CGGCAACTCT CACGGCCCAA GAGCGCGGAG 
CCACCCCGCT CACCACTACT TAAGAGGGTG CAGTCAGCTG AAAAACTGGC AGCTGCACTG 
GCTGCTTCTG AGAAGAAGCT CGCCACTTCT CGAAAACAGA GCCTTGACCT GCCCCACCCC 
GAGCTAAAGA AGGAATTGCC GCCCAGGGAA GTCAGCTCTC TGGAGGTAGT TGGGACCAGG 
AGTGTGCTGT CTGGAAAGGG GGCGCTGCCA GGGAAAGGGG TGTTGCAGGC TGCTCCCTCA 
CGGGCCTTAG GGACCCTCCG CCAGGACAGG GCTGAACGGC GAGAGTCGCT GCAGAAGCAG 
GAAGCCATCC GGGAGGTTGA CTCTTCAGAA GATGATACTG ATGAGGGGCC TGAGAACAGC 
CAAGGTGTTC AGGAGCCAAG CTTGGTACCC CACCGAGAAG GGGGCCAGGA TCCACCCCTC 
AGAGGAGCAG GAGAGGGTGA GGAAGAGGAT GCCTTCTTGC CCAGGGATCC CAGGGGACAG 
GGCCCAGTGG TCACAGGTCT ACTGACAGGG GTCACACTGG GATCTCCCAG AATGGAAGGC 
CCTGGTGTCC CCCAGAAGAA GCTTGGAATC CTACAGGCTT TTGAAGAGGG TTCCAGCTCC 
TCGTCCTCAG CCCCCAACCT GGGGAAGGCT GGCCCCACAG ACCCCATCCC CGCTGAAGGC 
TGCTGGAAGG CCCAGCACCT CCACACCCAG GCACTGACAG CACTTTGTCC GAGCTCATCA 
GGACGCATCC CTGCCAGTTG CTCCACTGCC TCCACCCCTG GAGAGCTGGG CCCATGGTCC 
TGGAAATTCC TCTTCAAGGG CCCTGACAGG GCATACCCAA CCGGAAAGGC AGCAATGGAA 
GATGGGCCAG CCAGCTCCCA AGATTTGGAA ACTACAACTC CAGGCCACCC TGCAAACCTG 
TCCCCCAGAC AGGAGGGGAA GTCATGGCCA CCCAGTGCCC CTGGGCTGGC CCATCCACCT 
TGTGAGGTTC CCAGCCAGAG CTGGCTATGG GAGTCTGAGT GTGCACAGAG AGAGAAAGAG 
GAGCCAGCGC TGGGCATCAC CAAAGTGCCT GATGCCTCAG GTGACAGGAG GCAGGACGTT 
CCATGCAAGA GCTGCCCCTT CACCCCAGAG CCTGGGCCCA GCCTGCTCCA GAAGGGCCAA 
GACCCAGGGG GCCCTCAGGA GCATCAGGAC TTGGCATTGT TACCTGATGA GCTTTTAAAG 
CAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318273.1
CDS	299..5707
Translation	MKRSRCRDRPQPPPPPLPQTAGREDGAQRAAELPQPQPLPPRRRAPPGRQRLEERTGPVGPEVREQDVVTGRSPLLFRKLSNPDIFSSTGKAKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSGSAHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQERKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_015462787.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462787.1

ORF Insert Sequence:

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA 
TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC 
TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG 
ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG 
CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA 
ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG 
CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA 
ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC 
CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT 
CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG 
AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC 
CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG 
ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA 
GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA 
GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA 
ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC 
ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC 
AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA 
GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC 
CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC 
CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT 
TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT 
GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA 
CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC 
ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC 
TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG 
GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA 
GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT 
CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT 
GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG 
GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC 
AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC 
AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG 
CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG 
TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC 
CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG 
CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG 
GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC 
CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC 
ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG 
GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC 
ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC 
ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC 
TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG 
TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG 
CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG 
GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG 
GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC 
ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG 
AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGAAAAA GGAGCTCCCT GTTCCGGAAG 
ATCACCAAGC AGGCATCCCT GCTCCACACC AGCCGCAGCC TTTCTTCCCT TAACCGCTCC 
CTGTCGTCCG GCGAGAGTGG GCCAGGCTCG CCCACACACA GCCACAGCCT TTCTCCCCGC 
TCTCCCACGC AGGGCTACCG GGTGACCCCT GAAGCCGTGC ACTCAGTGGG GGGGAATTCA 
TCACAGAGCA GCTCCCCCAG CTCCAGCGTG CCCAGTTCTC CTGCCGGCTC TGGGCACACA 
CGGCCGAGCT CCCTCCACGG TCTGGCGCCC AAGCTCCAAC GCCAGTACCG CTCTCCACGG 
CGCAAGTCAG CAGGCAGCAT CCCGCTGTCA CCACTGGCCC ACACCCCTTC ACCACCACCA 
GCGGCTTCGC CTCAGCGTTC CCCATCACCT CTGTCTGGCC ACGGGGCCCA GGCCTTCCCT 
ACCAAGCTCC ACTTGTCGCC TCCCCTAGGC CGGCAACTCT CACGGCCCAA GAGCGCGGAG 
CCACCCCGCT CACCACTACT TAAGAGGGTG CAGTCAGCTG AAAAACTGGC AGCTGCACTG 
GCTGCTTCTG AGAAGAAGCT CGCCACTTCT CGAAAACAGA GCCTTGACCT GCCCCACCCC 
GAGCTAAAGA AGGAATTGCC GCCCAGGGAA GTCAGCTCTC TGGAGGTAGT TGGGACCAGG 
AGTGTGCTGT CTGGAAAGGG GGCGCTGCCA GGGAAAGGGG TGTTGCAGGC TGCTCCCTCA 
CGGGCCTTAG GGACCCTCCG CCAGGACAGG GCTGAACGGC GAGAGTCGCT GCAGAAGCAG 
GAAGCCATCC GGGAGGTTGA CTCTTCAGAA GATGATACTG ATGAGGGGCC TGAGAACAGC 
CAAGGTGTTC AGGAGCCAAG CTTGGTACCC CACCGAGAAG GGGGCCAGGA TCCACCCCTC 
AGAGGAGCAG GAGAGGGTGA GGAAGAGGAT GCCTTCTTGC CCAGGGATCC CAGGGGACAG 
GGCCCAGTGG TCACAGGTCT ACTGACAGGG GTCACACTGG GATCTCCCAG AATGGAAGGC 
CCTGGTGTCC CCCAGAAGAA GCTTGGAATC CTACAGGCTT TTGAAGAGGG TTCCAGCTCC 
TCGTCCTCAG CCCCCAACCT GGGGAAGGCT GGCCCCACAG ACCCCATCCC CGCTGAAGGC 
TGCTGGAAGG CCCAGCACCT CCACACCCAG GCACTGACAG CACTTTGTCC GAGCTCATCA 
GGACGCATCC CTGCCAGTTG CTCCACTGCC TCCACCCCTG GAGAGCTGGG CCCATGGTCC 
TGGAAATTCC TCTTCAAGGG CCCTGACAGG GCATACCCAA CCGGAAAGGC AGCAATGGAA 
GATGGGCCAG CCAGCTCCCA AGATTTGGAA ACTACAACTC CAGGCCACCC TGCAAACCTG 
TCCCCCAGAC AGGAGGGGAA GTCATGGCCA CCCAGTGCCC CTGGGCTGGC CCATCCACCT 
TGTGAGGTTC CCAGCCAGAG CTGGCTATGG GAGTCTGAGT GTGCACAGAG AGAGAAAGAG 
GAGCCAGCGC TGGGCATCAC CAAAGTGCCT GATGCCTCAG GTGACAGGAG GCAGGACGTT 
CCATGCAAGA GCTGCCCCTT CACCCCAGAG CCTGGGCCCA GCCTGCTCCA GAAGGGCCAA 
GACCCAGGGG GCCCTCAGGA GCATCAGGAC TTGGCATTGT TACCTGATGA GCTTTTAAAG 
CAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_005204882.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204939.1 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.

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CloneID	OBa18161
Clone ID Related Accession (Same CDS sequence)	XM_015462787.1 , XM_005204882.2
Accession Version	XM_005204882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005204882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA 
TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC 
TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG 
ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG 
CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA 
ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG 
CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA 
ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC 
CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT 
CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG 
AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC 
CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG 
ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA 
GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA 
GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA 
ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC 
ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC 
AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA 
GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC 
CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC 
CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT 
TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT 
GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA 
CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC 
ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC 
TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG 
GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA 
GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT 
CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT 
GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG 
GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC 
AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC 
AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG 
CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG 
TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC 
CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG 
CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG 
GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC 
CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC 
ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG 
GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC 
ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC 
ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC 
TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG 
TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG 
CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG 
GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG 
GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC 
ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG 
AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGAAAAA GGAGCTCCCT GTTCCGGAAG 
ATCACCAAGC AGGCATCCCT GCTCCACACC AGCCGCAGCC TTTCTTCCCT TAACCGCTCC 
CTGTCGTCCG GCGAGAGTGG GCCAGGCTCG CCCACACACA GCCACAGCCT TTCTCCCCGC 
TCTCCCACGC AGGGCTACCG GGTGACCCCT GAAGCCGTGC ACTCAGTGGG GGGGAATTCA 
TCACAGAGCA GCTCCCCCAG CTCCAGCGTG CCCAGTTCTC CTGCCGGCTC TGGGCACACA 
CGGCCGAGCT CCCTCCACGG TCTGGCGCCC AAGCTCCAAC GCCAGTACCG CTCTCCACGG 
CGCAAGTCAG CAGGCAGCAT CCCGCTGTCA CCACTGGCCC ACACCCCTTC ACCACCACCA 
GCGGCTTCGC CTCAGCGTTC CCCATCACCT CTGTCTGGCC ACGGGGCCCA GGCCTTCCCT 
ACCAAGCTCC ACTTGTCGCC TCCCCTAGGC CGGCAACTCT CACGGCCCAA GAGCGCGGAG 
CCACCCCGCT CACCACTACT TAAGAGGGTG CAGTCAGCTG AAAAACTGGC AGCTGCACTG 
GCTGCTTCTG AGAAGAAGCT CGCCACTTCT CGAAAACAGA GCCTTGACCT GCCCCACCCC 
GAGCTAAAGA AGGAATTGCC GCCCAGGGAA GTCAGCTCTC TGGAGGTAGT TGGGACCAGG 
AGTGTGCTGT CTGGAAAGGG GGCGCTGCCA GGGAAAGGGG TGTTGCAGGC TGCTCCCTCA 
CGGGCCTTAG GGACCCTCCG CCAGGACAGG GCTGAACGGC GAGAGTCGCT GCAGAAGCAG 
GAAGCCATCC GGGAGGTTGA CTCTTCAGAA GATGATACTG ATGAGGGGCC TGAGAACAGC 
CAAGGTGTTC AGGAGCCAAG CTTGGTACCC CACCGAGAAG GGGGCCAGGA TCCACCCCTC 
AGAGGAGCAG GAGAGGGTGA GGAAGAGGAT GCCTTCTTGC CCAGGGATCC CAGGGGACAG 
GGCCCAGTGG TCACAGGTCT ACTGACAGGG GTCACACTGG GATCTCCCAG AATGGAAGGC 
CCTGGTGTCC CCCAGAAGAA GCTTGGAATC CTACAGGCTT TTGAAGAGGG TTCCAGCTCC 
TCGTCCTCAG CCCCCAACCT GGGGAAGGCT GGCCCCACAG ACCCCATCCC CGCTGAAGGC 
TGCTGGAAGG CCCAGCACCT CCACACCCAG GCACTGACAG CACTTTGTCC GAGCTCATCA 
GGACGCATCC CTGCCAGTTG CTCCACTGCC TCCACCCCTG GAGAGCTGGG CCCATGGTCC 
TGGAAATTCC TCTTCAAGGG CCCTGACAGG GCATACCCAA CCGGAAAGGC AGCAATGGAA 
GATGGGCCAG CCAGCTCCCA AGATTTGGAA ACTACAACTC CAGGCCACCC TGCAAACCTG 
TCCCCCAGAC AGGAGGGGAA GTCATGGCCA CCCAGTGCCC CTGGGCTGGC CCATCCACCT 
TGTGAGGTTC CCAGCCAGAG CTGGCTATGG GAGTCTGAGT GTGCACAGAG AGAGAAAGAG 
GAGCCAGCGC TGGGCATCAC CAAAGTGCCT GATGCCTCAG GTGACAGGAG GCAGGACGTT 
CCATGCAAGA GCTGCCCCTT CACCCCAGAG CCTGGGCCCA GCCTGCTCCA GAAGGGCCAA 
GACCCAGGGG GCCCTCAGGA GCATCAGGAC TTGGCATTGT TACCTGATGA GCTTTTAAAG 
CAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204939.1
CDS	299..5707
Translation	MKRSRCRDRPQPPPPPLPQTAGREDGAQRAAELPQPQPLPPRRRAPPGRQRLEERTGPVGPEVREQDVVTGRSPLLFRKLSNPDIFSSTGKAKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSGSAHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTDSVPDEEGRQSPALRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDNVLPLADGTLSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIVIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSYDSPDTPETDDSVEGRGASLTSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHVDSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDRTDGLAGLRGRERSWVIGSPEILRKRLSVSESSHTESDSSPPLTVRRHCSGLLDVPRFPESTEEAVGAPRRQQQEGAWLLTPSGEGASGPALERPAERRLKLNEEPAGQSSAPSPAMETRGGRGTPQLAEGATARAISDLAVRRARHRLLSGDSIEKRTARPINKVIKSASATALSLLIPAEHHSCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALSSSRPPIVIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQERKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPEAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPAASPQRSPSPLSGHGAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKQSLDLPHPELKKELPPREVSSLEVVGTRSVLSGKGALPGKGVLQAAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEGPENSQGVQEPSLVPHREGGQDPPLRGAGEGEEEDAFLPRDPRGQGPVVTGLLTGVTLGSPRMEGPGVPQKKLGILQAFEEGSSSSSSAPNLGKAGPTDPIPAEGCWKAQHLHTQALTALCPSSSGRIPASCSTASTPGELGPWSWKFLFKGPDRAYPTGKAAMEDGPASSQDLETTTPGHPANLSPRQEGKSWPPSAPGLAHPPCEVPSQSWLWESECAQREKEEPALGITKVPDASGDRRQDVPCKSCPFTPEPGPSLLQKGQDPGGPQEHQDLALLPDELLKQT

Target ORF information:

RefSeq Version	XM_005204882.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204882.2

ORF Insert Sequence:

ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC 
CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG 
CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC 
AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG 
GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC 
CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA 
GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC 
GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC 
AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC 
CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT 
GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA 
TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC 
TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG 
ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG 
CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA 
ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG 
CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA 
ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC 
CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT 
CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG 
AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC 
CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG 
ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA 
GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA 
GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA 
ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC 
ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC 
AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA 
GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC 
CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC 
CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT 
TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT 
GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA 
CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC 
ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC 
TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG 
GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA 
GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT 
CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT 
GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG 
GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC 
AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC 
AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG 
CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG 
TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC 
CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG 
CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG 
GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC 
CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC 
ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG 
GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC 
ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC 
ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC 
TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG 
TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG 
CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG 
GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG 
GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC 
ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG 
AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGAAAAA GGAGCTCCCT GTTCCGGAAG 
ATCACCAAGC AGGCATCCCT GCTCCACACC AGCCGCAGCC TTTCTTCCCT TAACCGCTCC 
CTGTCGTCCG GCGAGAGTGG GCCAGGCTCG CCCACACACA GCCACAGCCT TTCTCCCCGC 
TCTCCCACGC AGGGCTACCG GGTGACCCCT GAAGCCGTGC ACTCAGTGGG GGGGAATTCA 
TCACAGAGCA GCTCCCCCAG CTCCAGCGTG CCCAGTTCTC CTGCCGGCTC TGGGCACACA 
CGGCCGAGCT CCCTCCACGG TCTGGCGCCC AAGCTCCAAC GCCAGTACCG CTCTCCACGG 
CGCAAGTCAG CAGGCAGCAT CCCGCTGTCA CCACTGGCCC ACACCCCTTC ACCACCACCA 
GCGGCTTCGC CTCAGCGTTC CCCATCACCT CTGTCTGGCC ACGGGGCCCA GGCCTTCCCT 
ACCAAGCTCC ACTTGTCGCC TCCCCTAGGC CGGCAACTCT CACGGCCCAA GAGCGCGGAG 
CCACCCCGCT CACCACTACT TAAGAGGGTG CAGTCAGCTG AAAAACTGGC AGCTGCACTG 
GCTGCTTCTG AGAAGAAGCT CGCCACTTCT CGAAAACAGA GCCTTGACCT GCCCCACCCC 
GAGCTAAAGA AGGAATTGCC GCCCAGGGAA GTCAGCTCTC TGGAGGTAGT TGGGACCAGG 
AGTGTGCTGT CTGGAAAGGG GGCGCTGCCA GGGAAAGGGG TGTTGCAGGC TGCTCCCTCA 
CGGGCCTTAG GGACCCTCCG CCAGGACAGG GCTGAACGGC GAGAGTCGCT GCAGAAGCAG 
GAAGCCATCC GGGAGGTTGA CTCTTCAGAA GATGATACTG ATGAGGGGCC TGAGAACAGC 
CAAGGTGTTC AGGAGCCAAG CTTGGTACCC CACCGAGAAG GGGGCCAGGA TCCACCCCTC 
AGAGGAGCAG GAGAGGGTGA GGAAGAGGAT GCCTTCTTGC CCAGGGATCC CAGGGGACAG 
GGCCCAGTGG TCACAGGTCT ACTGACAGGG GTCACACTGG GATCTCCCAG AATGGAAGGC 
CCTGGTGTCC CCCAGAAGAA GCTTGGAATC CTACAGGCTT TTGAAGAGGG TTCCAGCTCC 
TCGTCCTCAG CCCCCAACCT GGGGAAGGCT GGCCCCACAG ACCCCATCCC CGCTGAAGGC 
TGCTGGAAGG CCCAGCACCT CCACACCCAG GCACTGACAG CACTTTGTCC GAGCTCATCA 
GGACGCATCC CTGCCAGTTG CTCCACTGCC TCCACCCCTG GAGAGCTGGG CCCATGGTCC 
TGGAAATTCC TCTTCAAGGG CCCTGACAGG GCATACCCAA CCGGAAAGGC AGCAATGGAA 
GATGGGCCAG CCAGCTCCCA AGATTTGGAA ACTACAACTC CAGGCCACCC TGCAAACCTG 
TCCCCCAGAC AGGAGGGGAA GTCATGGCCA CCCAGTGCCC CTGGGCTGGC CCATCCACCT 
TGTGAGGTTC CCAGCCAGAG CTGGCTATGG GAGTCTGAGT GTGCACAGAG AGAGAAAGAG 
GAGCCAGCGC TGGGCATCAC CAAAGTGCCT GATGCCTCAG GTGACAGGAG GCAGGACGTT 
CCATGCAAGA GCTGCCCCTT CACCCCAGAG CCTGGGCCCA GCCTGCTCCA GAAGGGCCAA 
GACCCAGGGG GCCCTCAGGA GCATCAGGAC TTGGCATTGT TACCTGATGA GCTTTTAAAG 
CAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MAST2
Entrez Gene ID	535643
Full Name	microtubule associated serine/threonine kinase 2
General protein information	Preferred Names microtubule associated serine/threonine kinase 2 Names microtubule-associated serine/threonine-protein kinase 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Canis lupus familiaris (dog)	MAST2	XP_005629101.1
Mus musculus (house mouse)	Mast2	NP_001036208.1
Danio rerio (zebrafish)	mast2	XP_001341305.4
Pan troglodytes (chimpanzee)	MAST2	XP_513151.3
Rattus norvegicus (Norway rat)	Mast2	NP_001101475.1
Bos taurus (cattle)	MAST2	XP_005204937.1
Gallus gallus (chicken)	MAST2	XP_004936815.1
Homo sapiens (human)	MAST2	NP_055927.2
	MAST2	XP_001105315.1
Xenopus tropicalis (tropical clawed frog)	mast2	NP_001122121.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MAST2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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見積依頼