MAST2 cDNA ORF clone, Bos taurus(cattle)

The following MAST2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAST2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa233318 XM_005204880.3
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa233319 XM_005204881.3
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa233320 XM_003585907.4
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa233321 XM_005204882.3
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa233322 XM_005204883.4
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa233323 XM_005204884.4
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa53214 XM_024990152.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa18162 XM_005204883.3
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa18162 XM_015462788.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa18163 XM_005204884.3
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa18163 XM_015462789.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa18165 XM_005204886.4
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa18165 XM_015462790.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa53217 XM_010803741.3
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa53217 XM_003582019.4
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa18158 XM_015462784.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa18158 XM_005204880.2
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa18159 XM_015462785.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa18159 XM_005204881.2
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa18160 XM_015462786.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa18160 XM_003585907.3
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa18161 XM_005204882.2
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa18161 XM_015462787.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
OBa18165 XM_005204886.3 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa53217 XM_010803741.2 Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa233318
    Clone ID Related Accession (Same CDS sequence) XM_005204880.3
    Accession Version XM_005204880.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA
    CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT
    ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG
    GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG
    GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA
    GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG
    GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC
    GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC
    CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT
    CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG
    GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC
    CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC
    CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG
    GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT
    ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA
    CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG
    TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG
    AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT
    GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC
    ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC
    CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA
    CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG
    GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA
    AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC
    GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG
    GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT
    GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG
    GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC
    CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT
    GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA
    GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG
    GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT
    CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG
    ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG
    GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG
    CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG
    CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT
    GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG
    GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA
    CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC
    CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC
    CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA
    GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC
    TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG
    GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC
    ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT
    GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC
    AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC
    GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT
    TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC
    AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGCAGA
    AAAAGGAGCT CCCTGTTCCG GAAGATCACC AAGCAGGCAT CCCTGCTCCA CACCAGCCGC
    AGCCTTTCTT CCCTTAACCG CTCCCTGTCG TCCGGCGAGA GTGGGCCAGG CTCGCCCACA
    CACAGCCACA GCCTTTCTCC CCGCTCTCCC ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC
    GTGCACTCAG TGGGGGGGAA TTCATCACAG AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT
    TCTCCTGCCG GCTCTGGGCA CACACGGCCG AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC
    CAACGCCAGT ACCGCTCTCC ACGGCGCAAG TCAGCAGGCA GCATCCCGCT GTCACCACTG
    GCCCACACCC CTTCACCACC ACCAGCGGCT TCGCCTCAGC GTTCCCCATC ACCTCTGTCT
    GGCCACGGGG CCCAGGCCTT CCCTACCAAG CTCCACTTGT CGCCTCCCCT AGGCCGGCAA
    CTCTCACGGC CCAAGAGCGC GGAGCCACCC CGCTCACCAC TACTTAAGAG GGTGCAGTCA
    GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA
    CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC
    TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA
    GGGGTGTTGC AGGCTGCTCC CTCACGGGCC TTAGGGACCC TCCGCCAGGA CAGGGCTGAA
    CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC ATCCGGGAGG TTGACTCTTC AGAAGATGAT
    ACTGATGAGG GGCCTGAGAA CAGCCAAGGT GTTCAGGAGC CAAGCTTGGT ACCCCACCGA
    GAAGGGGGCC AGGATCCACC CCTCAGAGGA GCAGGAGAGG GTGAGGAAGA GGATGCCTTC
    TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA GTGGTCACAG GTCTACTGAC AGGGGTCACA
    CTGGGATCTC CCAGAATGGA AGGCCCTGGT GTCCCCCAGA AGAAGCTTGG AATCCTACAG
    GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC
    ACAGACCCCA TCCCCGCTGA AGGCTGCTGG AAGGCCCAGC ACCTCCACAC CCAGGCACTG
    ACAGCACTTT GTCCGAGCTC ATCAGGACGC ATCCCTGCCA GTTGCTCCAC TGCCTCCACC
    CCTGGAGAGC TGGGCCCATG GTCCTGGAAA TTCCTCTTCA AGGGCCCTGA CAGGGCATAC
    CCAACCGGAA AGGCAGCAAT GGAAGATGGG CCAGCCAGCT CCCAAGATTT GGAAACTACA
    ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC AGACAGGAGG GGAAGTCATG GCCACCCAGT
    GCCCCTGGGC TGGCCCATCC ACCTTGTGAG GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT
    GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA GCGCTGGGCA TCACCAAAGT GCCTGATGCC
    TCAGGTGACA GGAGGCAGGA CGTTCCATGC AAGAGCTGCC CCTTCACCCC AGAGCCTGGG
    CCCAGCCTGC TCCAGAAGGG CCAAGACCCA GGGGGCCCTC AGGAGCATCA GGACTTGGCA
    TTGTTACCTG ATGAGCTTTT AAAGCAAACA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005204937.2
    CDS307..5739
    Translation

    Target ORF information:

    RefSeq Version XM_005204880.3
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204880.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA
    CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT
    ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG
    GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG
    GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA
    GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG
    GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC
    GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC
    CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT
    CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG
    GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC
    CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC
    CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG
    GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT
    ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA
    CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG
    TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG
    AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT
    GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC
    ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC
    CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA
    CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG
    GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA
    AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC
    GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG
    GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT
    GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG
    GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC
    CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT
    GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA
    GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG
    GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT
    CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG
    ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG
    GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG
    CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG
    CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT
    GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG
    GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA
    CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC
    CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC
    CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA
    GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC
    TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG
    GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC
    ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT
    GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC
    AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC
    GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT
    TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC
    AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGCAGA
    AAAAGGAGCT CCCTGTTCCG GAAGATCACC AAGCAGGCAT CCCTGCTCCA CACCAGCCGC
    AGCCTTTCTT CCCTTAACCG CTCCCTGTCG TCCGGCGAGA GTGGGCCAGG CTCGCCCACA
    CACAGCCACA GCCTTTCTCC CCGCTCTCCC ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC
    GTGCACTCAG TGGGGGGGAA TTCATCACAG AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT
    TCTCCTGCCG GCTCTGGGCA CACACGGCCG AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC
    CAACGCCAGT ACCGCTCTCC ACGGCGCAAG TCAGCAGGCA GCATCCCGCT GTCACCACTG
    GCCCACACCC CTTCACCACC ACCAGCGGCT TCGCCTCAGC GTTCCCCATC ACCTCTGTCT
    GGCCACGGGG CCCAGGCCTT CCCTACCAAG CTCCACTTGT CGCCTCCCCT AGGCCGGCAA
    CTCTCACGGC CCAAGAGCGC GGAGCCACCC CGCTCACCAC TACTTAAGAG GGTGCAGTCA
    GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA
    CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC
    TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA
    GGGGTGTTGC AGGCTGCTCC CTCACGGGCC TTAGGGACCC TCCGCCAGGA CAGGGCTGAA
    CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC ATCCGGGAGG TTGACTCTTC AGAAGATGAT
    ACTGATGAGG GGCCTGAGAA CAGCCAAGGT GTTCAGGAGC CAAGCTTGGT ACCCCACCGA
    GAAGGGGGCC AGGATCCACC CCTCAGAGGA GCAGGAGAGG GTGAGGAAGA GGATGCCTTC
    TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA GTGGTCACAG GTCTACTGAC AGGGGTCACA
    CTGGGATCTC CCAGAATGGA AGGCCCTGGT GTCCCCCAGA AGAAGCTTGG AATCCTACAG
    GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC
    ACAGACCCCA TCCCCGCTGA AGGCTGCTGG AAGGCCCAGC ACCTCCACAC CCAGGCACTG
    ACAGCACTTT GTCCGAGCTC ATCAGGACGC ATCCCTGCCA GTTGCTCCAC TGCCTCCACC
    CCTGGAGAGC TGGGCCCATG GTCCTGGAAA TTCCTCTTCA AGGGCCCTGA CAGGGCATAC
    CCAACCGGAA AGGCAGCAAT GGAAGATGGG CCAGCCAGCT CCCAAGATTT GGAAACTACA
    ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC AGACAGGAGG GGAAGTCATG GCCACCCAGT
    GCCCCTGGGC TGGCCCATCC ACCTTGTGAG GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT
    GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA GCGCTGGGCA TCACCAAAGT GCCTGATGCC
    TCAGGTGACA GGAGGCAGGA CGTTCCATGC AAGAGCTGCC CCTTCACCCC AGAGCCTGGG
    CCCAGCCTGC TCCAGAAGGG CCAAGACCCA GGGGGCCCTC AGGAGCATCA GGACTTGGCA
    TTGTTACCTG ATGAGCTTTT AAAGCAAACA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233319
    Clone ID Related Accession (Same CDS sequence) XM_005204881.3
    Accession Version XM_005204881.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204881.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA
    CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT
    ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG
    GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG
    GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA
    GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG
    GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC
    GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC
    CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT
    CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG
    GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC
    CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC
    CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG
    GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT
    ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA
    CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG
    TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG
    AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT
    GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC
    ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC
    CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA
    CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG
    GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA
    AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC
    GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG
    GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT
    GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG
    GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC
    CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT
    GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA
    GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG
    GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT
    CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG
    ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG
    GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG
    CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG
    CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT
    GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG
    GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA
    CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC
    CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC
    CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA
    GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC
    TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG
    GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC
    ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT
    GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC
    AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC
    GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT
    TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC
    AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGAAAA
    AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC
    CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC
    AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG
    CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT
    CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA
    CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC
    CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC
    CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC
    TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT
    GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG
    AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT
    CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG
    GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG
    CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT
    GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA
    GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG
    CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG
    GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT
    TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA
    GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA
    GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT
    GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA
    ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT
    CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC
    CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG
    TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA
    GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC
    AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG
    TTACCTGATG AGCTTTTAAA GCAAACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005204938.2
    CDS307..5736
    Translation

    Target ORF information:

    RefSeq Version XM_005204881.3
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204881.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA
    CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT
    ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG
    GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG
    GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA
    GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG
    GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC
    GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC
    CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT
    CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG
    GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC
    CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC
    CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG
    GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT
    ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA
    CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG
    TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG
    AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT
    GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC
    ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC
    CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA
    CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG
    GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA
    AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC
    GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG
    GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT
    GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG
    GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC
    CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT
    GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA
    GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG
    GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT
    CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG
    ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG
    GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG
    CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG
    CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT
    GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG
    GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA
    CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC
    CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC
    CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA
    GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC
    TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG
    GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC
    ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT
    GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC
    AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC
    GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT
    TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC
    AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGAAAA
    AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC
    CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC
    AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG
    CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT
    CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA
    CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC
    CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC
    CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC
    TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT
    GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG
    AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT
    CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG
    GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG
    CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT
    GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA
    GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG
    CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG
    GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT
    TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA
    GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA
    GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT
    GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA
    ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT
    CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC
    CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG
    TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA
    GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC
    AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG
    TTACCTGATG AGCTTTTAAA GCAAACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233320
    Clone ID Related Accession (Same CDS sequence) XM_003585907.4
    Accession Version XM_003585907.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003585907.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA
    TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC
    TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG
    ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG
    CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA
    ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG
    CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA
    ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC
    CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT
    CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG
    AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC
    CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG
    ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA
    GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA
    GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA
    ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC
    ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG
    ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC
    AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA
    GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC
    CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC
    CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT
    TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT
    GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA
    CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC
    ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC
    TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG
    GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA
    GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT
    CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT
    GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG
    GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC
    AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC
    AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG
    CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG
    TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC
    CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG
    CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG
    GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC
    CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC
    ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG
    GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC
    ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC
    ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC
    TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG
    TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG
    CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG
    GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG
    GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC
    ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG
    AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG
    AAGATCACCA AGCAGGCATC CCTGCTCCAC ACCAGCCGCA GCCTTTCTTC CCTTAACCGC
    TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC TCGCCCACAC ACAGCCACAG CCTTTCTCCC
    CGCTCTCCCA CGCAGGGCTA CCGGGTGACC CCTGAAGCCG TGCACTCAGT GGGGGGGAAT
    TCATCACAGA GCAGCTCCCC CAGCTCCAGC GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC
    ACACGGCCGA GCTCCCTCCA CGGTCTGGCG CCCAAGCTCC AACGCCAGTA CCGCTCTCCA
    CGGCGCAAGT CAGCAGGCAG CATCCCGCTG TCACCACTGG CCCACACCCC TTCACCACCA
    CCAGCGGCTT CGCCTCAGCG TTCCCCATCA CCTCTGTCTG GCCACGGGGC CCAGGCCTTC
    CCTACCAAGC TCCACTTGTC GCCTCCCCTA GGCCGGCAAC TCTCACGGCC CAAGAGCGCG
    GAGCCACCCC GCTCACCACT ACTTAAGAGG GTGCAGTCAG CTGAAAAACT GGCAGCTGCA
    CTGGCTGCTT CTGAGAAGAA GCTCGCCACT TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC
    CCCGAGCTAA AGAAGGAATT GCCGCCCAGG GAAGTCAGCT CTCTGGAGGT AGTTGGGACC
    AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC
    TCACGGGCCT TAGGGACCCT CCGCCAGGAC AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG
    CAGGAAGCCA TCCGGGAGGT TGACTCTTCA GAAGATGATA CTGATGAGGG GCCTGAGAAC
    AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA CCCCACCGAG AAGGGGGCCA GGATCCACCC
    CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG GATGCCTTCT TGCCCAGGGA TCCCAGGGGA
    CAGGGCCCAG TGGTCACAGG TCTACTGACA GGGGTCACAC TGGGATCTCC CAGAATGGAA
    GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA ATCCTACAGG CTTTTGAAGA GGGTTCCAGC
    TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG GCTGGCCCCA CAGACCCCAT CCCCGCTGAA
    GGCTGCTGGA AGGCCCAGCA CCTCCACACC CAGGCACTGA CAGCACTTTG TCCGAGCTCA
    TCAGGACGCA TCCCTGCCAG TTGCTCCACT GCCTCCACCC CTGGAGAGCT GGGCCCATGG
    TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC AGGGCATACC CAACCGGAAA GGCAGCAATG
    GAAGATGGGC CAGCCAGCTC CCAAGATTTG GAAACTACAA CTCCAGGCCA CCCTGCAAAC
    CTGTCCCCCA GACAGGAGGG GAAGTCATGG CCACCCAGTG CCCCTGGGCT GGCCCATCCA
    CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA TGGGAGTCTG AGTGTGCACA GAGAGAGAAA
    GAGGAGCCAG CGCTGGGCAT CACCAAAGTG CCTGATGCCT CAGGTGACAG GAGGCAGGAC
    GTTCCATGCA AGAGCTGCCC CTTCACCCCA GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC
    CAAGACCCAG GGGGCCCTCA GGAGCATCAG GACTTGGCAT TGTTACCTGA TGAGCTTTTA
    AAGCAAACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003585955.2
    CDS307..5718
    Translation

    Target ORF information:

    RefSeq Version XM_003585907.4
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003585907.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA
    TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC
    TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG
    ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG
    CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA
    ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG
    CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA
    ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC
    CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT
    CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG
    AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC
    CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG
    ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA
    GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA
    GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA
    ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC
    ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG
    ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC
    AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA
    GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC
    CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC
    CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT
    TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT
    GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA
    CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC
    ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC
    TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG
    GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA
    GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT
    CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT
    GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG
    GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC
    AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC
    AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG
    CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG
    TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC
    CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG
    CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG
    GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC
    CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC
    ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG
    GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC
    ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC
    ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC
    TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG
    TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG
    CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG
    GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG
    GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC
    ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG
    AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG
    AAGATCACCA AGCAGGCATC CCTGCTCCAC ACCAGCCGCA GCCTTTCTTC CCTTAACCGC
    TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC TCGCCCACAC ACAGCCACAG CCTTTCTCCC
    CGCTCTCCCA CGCAGGGCTA CCGGGTGACC CCTGAAGCCG TGCACTCAGT GGGGGGGAAT
    TCATCACAGA GCAGCTCCCC CAGCTCCAGC GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC
    ACACGGCCGA GCTCCCTCCA CGGTCTGGCG CCCAAGCTCC AACGCCAGTA CCGCTCTCCA
    CGGCGCAAGT CAGCAGGCAG CATCCCGCTG TCACCACTGG CCCACACCCC TTCACCACCA
    CCAGCGGCTT CGCCTCAGCG TTCCCCATCA CCTCTGTCTG GCCACGGGGC CCAGGCCTTC
    CCTACCAAGC TCCACTTGTC GCCTCCCCTA GGCCGGCAAC TCTCACGGCC CAAGAGCGCG
    GAGCCACCCC GCTCACCACT ACTTAAGAGG GTGCAGTCAG CTGAAAAACT GGCAGCTGCA
    CTGGCTGCTT CTGAGAAGAA GCTCGCCACT TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC
    CCCGAGCTAA AGAAGGAATT GCCGCCCAGG GAAGTCAGCT CTCTGGAGGT AGTTGGGACC
    AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC
    TCACGGGCCT TAGGGACCCT CCGCCAGGAC AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG
    CAGGAAGCCA TCCGGGAGGT TGACTCTTCA GAAGATGATA CTGATGAGGG GCCTGAGAAC
    AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA CCCCACCGAG AAGGGGGCCA GGATCCACCC
    CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG GATGCCTTCT TGCCCAGGGA TCCCAGGGGA
    CAGGGCCCAG TGGTCACAGG TCTACTGACA GGGGTCACAC TGGGATCTCC CAGAATGGAA
    GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA ATCCTACAGG CTTTTGAAGA GGGTTCCAGC
    TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG GCTGGCCCCA CAGACCCCAT CCCCGCTGAA
    GGCTGCTGGA AGGCCCAGCA CCTCCACACC CAGGCACTGA CAGCACTTTG TCCGAGCTCA
    TCAGGACGCA TCCCTGCCAG TTGCTCCACT GCCTCCACCC CTGGAGAGCT GGGCCCATGG
    TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC AGGGCATACC CAACCGGAAA GGCAGCAATG
    GAAGATGGGC CAGCCAGCTC CCAAGATTTG GAAACTACAA CTCCAGGCCA CCCTGCAAAC
    CTGTCCCCCA GACAGGAGGG GAAGTCATGG CCACCCAGTG CCCCTGGGCT GGCCCATCCA
    CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA TGGGAGTCTG AGTGTGCACA GAGAGAGAAA
    GAGGAGCCAG CGCTGGGCAT CACCAAAGTG CCTGATGCCT CAGGTGACAG GAGGCAGGAC
    GTTCCATGCA AGAGCTGCCC CTTCACCCCA GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC
    CAAGACCCAG GGGGCCCTCA GGAGCATCAG GACTTGGCAT TGTTACCTGA TGAGCTTTTA
    AAGCAAACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233321
    Clone ID Related Accession (Same CDS sequence) XM_005204882.3
    Accession Version XM_005204882.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204882.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA
    TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC
    TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG
    ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG
    CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA
    ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG
    CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA
    ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC
    CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT
    CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG
    AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC
    CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG
    ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA
    GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA
    GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA
    ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC
    ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG
    ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC
    AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA
    GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC
    CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC
    CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT
    TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT
    GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA
    CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC
    ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC
    TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG
    GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA
    GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT
    CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT
    GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG
    GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC
    AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC
    AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG
    CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG
    TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC
    CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG
    CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG
    GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC
    CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC
    ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG
    GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC
    ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC
    ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC
    TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG
    TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG
    CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG
    GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG
    GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC
    ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG
    AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGAAAAA GGAGCTCCCT GTTCCGGAAG
    ATCACCAAGC AGGCATCCCT GCTCCACACC AGCCGCAGCC TTTCTTCCCT TAACCGCTCC
    CTGTCGTCCG GCGAGAGTGG GCCAGGCTCG CCCACACACA GCCACAGCCT TTCTCCCCGC
    TCTCCCACGC AGGGCTACCG GGTGACCCCT GAAGCCGTGC ACTCAGTGGG GGGGAATTCA
    TCACAGAGCA GCTCCCCCAG CTCCAGCGTG CCCAGTTCTC CTGCCGGCTC TGGGCACACA
    CGGCCGAGCT CCCTCCACGG TCTGGCGCCC AAGCTCCAAC GCCAGTACCG CTCTCCACGG
    CGCAAGTCAG CAGGCAGCAT CCCGCTGTCA CCACTGGCCC ACACCCCTTC ACCACCACCA
    GCGGCTTCGC CTCAGCGTTC CCCATCACCT CTGTCTGGCC ACGGGGCCCA GGCCTTCCCT
    ACCAAGCTCC ACTTGTCGCC TCCCCTAGGC CGGCAACTCT CACGGCCCAA GAGCGCGGAG
    CCACCCCGCT CACCACTACT TAAGAGGGTG CAGTCAGCTG AAAAACTGGC AGCTGCACTG
    GCTGCTTCTG AGAAGAAGCT CGCCACTTCT CGAAAACAGA GCCTTGACCT GCCCCACCCC
    GAGCTAAAGA AGGAATTGCC GCCCAGGGAA GTCAGCTCTC TGGAGGTAGT TGGGACCAGG
    AGTGTGCTGT CTGGAAAGGG GGCGCTGCCA GGGAAAGGGG TGTTGCAGGC TGCTCCCTCA
    CGGGCCTTAG GGACCCTCCG CCAGGACAGG GCTGAACGGC GAGAGTCGCT GCAGAAGCAG
    GAAGCCATCC GGGAGGTTGA CTCTTCAGAA GATGATACTG ATGAGGGGCC TGAGAACAGC
    CAAGGTGTTC AGGAGCCAAG CTTGGTACCC CACCGAGAAG GGGGCCAGGA TCCACCCCTC
    AGAGGAGCAG GAGAGGGTGA GGAAGAGGAT GCCTTCTTGC CCAGGGATCC CAGGGGACAG
    GGCCCAGTGG TCACAGGTCT ACTGACAGGG GTCACACTGG GATCTCCCAG AATGGAAGGC
    CCTGGTGTCC CCCAGAAGAA GCTTGGAATC CTACAGGCTT TTGAAGAGGG TTCCAGCTCC
    TCGTCCTCAG CCCCCAACCT GGGGAAGGCT GGCCCCACAG ACCCCATCCC CGCTGAAGGC
    TGCTGGAAGG CCCAGCACCT CCACACCCAG GCACTGACAG CACTTTGTCC GAGCTCATCA
    GGACGCATCC CTGCCAGTTG CTCCACTGCC TCCACCCCTG GAGAGCTGGG CCCATGGTCC
    TGGAAATTCC TCTTCAAGGG CCCTGACAGG GCATACCCAA CCGGAAAGGC AGCAATGGAA
    GATGGGCCAG CCAGCTCCCA AGATTTGGAA ACTACAACTC CAGGCCACCC TGCAAACCTG
    TCCCCCAGAC AGGAGGGGAA GTCATGGCCA CCCAGTGCCC CTGGGCTGGC CCATCCACCT
    TGTGAGGTTC CCAGCCAGAG CTGGCTATGG GAGTCTGAGT GTGCACAGAG AGAGAAAGAG
    GAGCCAGCGC TGGGCATCAC CAAAGTGCCT GATGCCTCAG GTGACAGGAG GCAGGACGTT
    CCATGCAAGA GCTGCCCCTT CACCCCAGAG CCTGGGCCCA GCCTGCTCCA GAAGGGCCAA
    GACCCAGGGG GCCCTCAGGA GCATCAGGAC TTGGCATTGT TACCTGATGA GCTTTTAAAG
    CAAACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005204939.2
    CDS307..5715
    Translation

    Target ORF information:

    RefSeq Version XM_005204882.3
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204882.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGGCA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCTCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA
    TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC
    TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG
    ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG
    CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA
    ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG
    CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA
    ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC
    CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT
    CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG
    AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC
    CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG
    ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA
    GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA
    GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA
    ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC
    ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG
    ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC
    AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA
    GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC
    CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC
    CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT
    TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT
    GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA
    CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC
    ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC
    TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG
    GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA
    GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT
    CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT
    GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG
    GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC
    AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC
    AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG
    CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG
    TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC
    CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG
    CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG
    GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC
    CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC
    ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG
    GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC
    ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC
    ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC
    TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG
    TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG
    CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG
    GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG
    GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC
    ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG
    AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGAAAAA GGAGCTCCCT GTTCCGGAAG
    ATCACCAAGC AGGCATCCCT GCTCCACACC AGCCGCAGCC TTTCTTCCCT TAACCGCTCC
    CTGTCGTCCG GCGAGAGTGG GCCAGGCTCG CCCACACACA GCCACAGCCT TTCTCCCCGC
    TCTCCCACGC AGGGCTACCG GGTGACCCCT GAAGCCGTGC ACTCAGTGGG GGGGAATTCA
    TCACAGAGCA GCTCCCCCAG CTCCAGCGTG CCCAGTTCTC CTGCCGGCTC TGGGCACACA
    CGGCCGAGCT CCCTCCACGG TCTGGCGCCC AAGCTCCAAC GCCAGTACCG CTCTCCACGG
    CGCAAGTCAG CAGGCAGCAT CCCGCTGTCA CCACTGGCCC ACACCCCTTC ACCACCACCA
    GCGGCTTCGC CTCAGCGTTC CCCATCACCT CTGTCTGGCC ACGGGGCCCA GGCCTTCCCT
    ACCAAGCTCC ACTTGTCGCC TCCCCTAGGC CGGCAACTCT CACGGCCCAA GAGCGCGGAG
    CCACCCCGCT CACCACTACT TAAGAGGGTG CAGTCAGCTG AAAAACTGGC AGCTGCACTG
    GCTGCTTCTG AGAAGAAGCT CGCCACTTCT CGAAAACAGA GCCTTGACCT GCCCCACCCC
    GAGCTAAAGA AGGAATTGCC GCCCAGGGAA GTCAGCTCTC TGGAGGTAGT TGGGACCAGG
    AGTGTGCTGT CTGGAAAGGG GGCGCTGCCA GGGAAAGGGG TGTTGCAGGC TGCTCCCTCA
    CGGGCCTTAG GGACCCTCCG CCAGGACAGG GCTGAACGGC GAGAGTCGCT GCAGAAGCAG
    GAAGCCATCC GGGAGGTTGA CTCTTCAGAA GATGATACTG ATGAGGGGCC TGAGAACAGC
    CAAGGTGTTC AGGAGCCAAG CTTGGTACCC CACCGAGAAG GGGGCCAGGA TCCACCCCTC
    AGAGGAGCAG GAGAGGGTGA GGAAGAGGAT GCCTTCTTGC CCAGGGATCC CAGGGGACAG
    GGCCCAGTGG TCACAGGTCT ACTGACAGGG GTCACACTGG GATCTCCCAG AATGGAAGGC
    CCTGGTGTCC CCCAGAAGAA GCTTGGAATC CTACAGGCTT TTGAAGAGGG TTCCAGCTCC
    TCGTCCTCAG CCCCCAACCT GGGGAAGGCT GGCCCCACAG ACCCCATCCC CGCTGAAGGC
    TGCTGGAAGG CCCAGCACCT CCACACCCAG GCACTGACAG CACTTTGTCC GAGCTCATCA
    GGACGCATCC CTGCCAGTTG CTCCACTGCC TCCACCCCTG GAGAGCTGGG CCCATGGTCC
    TGGAAATTCC TCTTCAAGGG CCCTGACAGG GCATACCCAA CCGGAAAGGC AGCAATGGAA
    GATGGGCCAG CCAGCTCCCA AGATTTGGAA ACTACAACTC CAGGCCACCC TGCAAACCTG
    TCCCCCAGAC AGGAGGGGAA GTCATGGCCA CCCAGTGCCC CTGGGCTGGC CCATCCACCT
    TGTGAGGTTC CCAGCCAGAG CTGGCTATGG GAGTCTGAGT GTGCACAGAG AGAGAAAGAG
    GAGCCAGCGC TGGGCATCAC CAAAGTGCCT GATGCCTCAG GTGACAGGAG GCAGGACGTT
    CCATGCAAGA GCTGCCCCTT CACCCCAGAG CCTGGGCCCA GCCTGCTCCA GAAGGGCCAA
    GACCCAGGGG GCCCTCAGGA GCATCAGGAC TTGGCATTGT TACCTGATGA GCTTTTAAAG
    CAAACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233322
    Clone ID Related Accession (Same CDS sequence) XM_005204883.4
    Accession Version XM_005204883.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204883.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
    CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG
    TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT
    TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGTCCCT GTTCCAGCCG CTCACTGCAG
    TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA
    CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC
    CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGCCATGGC
    CACAGAGCAG ACAGGACTGA TGGACGGCGC TGGTCTTTGG CCTCATTGCC TTCTTCAGGC
    TATGGAACCA ACACCCCTAG TTCTACCGTG TCATCGTCGT GCTCCTCTCA GGAGAAGCTG
    CACCAGTTGC CTTTCCAGCC CACGGCCGAT GAGCTGCACT TCTTGACGAA GCATTTCAGC
    ACAGACAGTG TGCCTGATGA GGAGGGGCGG CAGTCCCCCG CCCTGCGGCC CCGCTCCCGC
    AGCCTCAGTC CTGGACGGTC ACCAGTTTCC TTTGACAGTG AAATAATAAT GATGAATCAT
    GTATACAAGG AAAGATTCCC CAAGGCCACT GCACAAATGG AAGAGCGTCT GGCCGAGTTC
    ATCTCCTCCA ATACTCCAGA CAACGTGTTG CCCTTGGCTG ATGGAACCTT GAGCTTTATT
    CATCATCAGG TGATAGAGAT GGCCCGAGAC TGCCTGGACA AATCCCGGAG TGGCCTCATT
    ACCTCACACT ACTTCTACGA ACTTCAAGAG AATTTAGAGA AGCTTCTGCA AGATGCTCAT
    GAGCGCTCAG AGAGTTCAGA AGTGGCTTTT GTGATGCAGC TGGTGAAAAA GCTGATGATT
    GTCATTGCCC GCCCAGCTCG TCTCCTGGAA TGCCTGGAGT TTGACCCTGA AGAGTTCTAC
    CACCTGTTAG AAGCAGCTGA GGGCCACGCC AAAGAGGGAC AAGGGATTAA ATGTGACATT
    CCCCGCTACA TCGTTAGCCA ACTGGGCCTC ACACGGGATC CCCTAGAGGA AATGGCCCAG
    TTGAGCAGCT ATGACAGTCC TGATACTCCG GAGACAGATG ATTCAGTCGA GGGCCGTGGG
    GCATCTCTGA CATCGAAAAA GACACCCTCT GAGGAGGACT TCGAAACCAT TAAGCTCATC
    AGCAATGGCG CCTATGGGGC CGTGTTCCTG GTGCGGCACA AGTCCACCCG GCAGCGCTTT
    GCCATGAAGA AGATCAACAA GCAGAACCTG ATCCTGCGGA ACCAGATCCA GCAGGCCTTC
    GTGGAGCGGG ACATCCTGAC TTTTGCTGAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC
    TTTGAAACCA AGCGCCACCT GTGCATGGTG ATGGAGTATG TGGAAGGAGG AGACTGTGCC
    ACGCTGCTGA AGAACATCGG GGCCCTGCCT GTGGACATGG TGCGCCTGTA CTTTGCAGAA
    ACTGTGCTAG CCCTGGAGTA CTTACACAAT TACGGCATCG TGCACCGTGA CCTCAAGCCT
    GACAACCTGC TGATTACATC CATGGGTCAC ATCAAGCTCA CTGATTTTGG CCTTTCCAAA
    ATTGGCCTTA TGAGTCTGAC CACAAACTTG TACGAGGGCC ACATTGAAAA GGATGCACGG
    GAGTTCCTGG ACAAGCAGGT ATGCGGGACC CCGGAGTACA TTGCACCTGA GGTGATCCTG
    CGGCAGGGCT ACGGGAAGCC AGTGGACTGG TGGGCTATGG GCATCATCCT GTACGAGTTC
    CTGGTGGGCT GCGTCCCTTT CTTTGGAGAC ACTCCGGAGG AGCTCTTTGG GCAAGTGATC
    AGTGATGAGA TTGTGTGGCC TGAGGGGGAC GATGCACTGC CGCCGGACGC CCAAGATCTC
    ACCTCAAAAC TGCTCCACCA GAACCCATTG GAGAGACTGG GCACAGGCAG TGCCTATGAG
    GTGAAGCAGC ACCCATTCTT CACTGGTCTG GACTGGACGG GACTTCTTCG CCAGAAGGCT
    GAATTTATTC CTCAGCTGGA GTCAGAGGAT GATACCAGCT ACTTTGACAC CCGCTCAGAG
    CGATACCACC ACGTGGACTC GGAGGATGAG GAGGAAGTGA GTGAGGATGG CTGCCTTGAG
    ATTCGCCAGT TCTCGTCCTG TTCTCCAAGG TTCAGCAAGG TGTACAGCAG CATGGAGCGG
    CTCTCGCTGC TCGAGGAGCG CCGGACGCCA CCCCCCACCA AGCGCAGCCT GAGTGAGGAA
    AAGGAGGACC GCACGGATGG CCTGGCGGGG CTGAGGGGCC GAGAGCGAAG CTGGGTGATC
    GGCTCCCCTG AGATATTACG GAAGCGGCTG TCGGTGTCCG AGTCGTCCCA CACGGAAAGC
    GATTCCAGCC CTCCACTCAC CGTGCGGCGC CACTGCTCGG GCCTCCTGGA CGTGCCTCGC
    TTCCCTGAGA GCACTGAGGA GGCTGTTGGC GCTCCCAGGA GGCAGCAGCA GGAGGGTGCA
    TGGCTTCTGA CCCCGTCTGG AGAGGGGGCG TCTGGGCCTG CCCTGGAACG GCCGGCCGAG
    CGGCGGCTGA AGCTGAATGA GGAACCTGCT GGCCAGAGCA GCGCCCCCAG CCCAGCCATG
    GAGACCCGAG GAGGCCGCGG AACCCCACAG CTGGCAGAAG GAGCCACAGC CAGGGCCATC
    AGCGACCTGG CGGTGCGCAG GGCCCGCCAC CGGCTGCTCT CTGGGGACTC CATAGAAAAG
    CGCACCGCTC GCCCCATCAA CAAAGTGATC AAGTCCGCCT CAGCCACAGC CCTGTCCCTT
    CTCATTCCTG CTGAGCACCA CTCCTGCTCT CCACTGGCCA GCCCCATGTC CCCACATTCC
    CAGTCATCCA ACCCATCATC CAGGGACTCT TCTCCAAGCA GGGACTTCCT GCCAGCCCTC
    AGCAGCTCAC GGCCCCCCAT TGTCATCCAC CGAGCCGGCA AGAAGTATGG CTTCACCCTA
    CGAGCCATCC GTGTCTACAT GGGTGACTCA GATGTCTACA CCGTGCACCA CATGGTGTGG
    CACGTGGAGG ACGGAGGTCC AGCCAGTGAG GCAGGGCTGC GTCAGGGGGA CCTCATCACG
    CACGTCAACG GGGAGCCTGT GCACGGGCTG GTGCACACGG AGGTGGTGGA GCTGATCCTG
    AAGAGTGGAA ACAAGGTGTC TATTTCAACA ACTCCCCTGG AGAACACCTC CATTAAGGTG
    GGGCCCGCTC GGAAGGGCAG CTACAAGGCC AAGATGGCCC GAAGGAGCAA GCGGAGCCGG
    GGCAAGGATG GACAAGAAAG CAGAAAAAGG AGCTCCCTGT TCCGGAAGAT CACCAAGCAG
    GCATCCCTGC TCCACACCAG CCGCAGCCTT TCTTCCCTTA ACCGCTCCCT GTCGTCCGGC
    GAGAGTGGGC CAGGCTCGCC CACACACAGC CACAGCCTTT CTCCCCGCTC TCCCACGCAG
    GGCTACCGGG TGACCCCTGA AGCCGTGCAC TCAGTGGGGG GGAATTCATC ACAGAGCAGC
    TCCCCCAGCT CCAGCGTGCC CAGTTCTCCT GCCGGCTCTG GGCACACACG GCCGAGCTCC
    CTCCACGGTC TGGCGCCCAA GCTCCAACGC CAGTACCGCT CTCCACGGCG CAAGTCAGCA
    GGCAGCATCC CGCTGTCACC ACTGGCCCAC ACCCCTTCAC CACCACCAGC GGCTTCGCCT
    CAGCGTTCCC CATCACCTCT GTCTGGCCAC GGGGCCCAGG CCTTCCCTAC CAAGCTCCAC
    TTGTCGCCTC CCCTAGGCCG GCAACTCTCA CGGCCCAAGA GCGCGGAGCC ACCCCGCTCA
    CCACTACTTA AGAGGGTGCA GTCAGCTGAA AAACTGGCAG CTGCACTGGC TGCTTCTGAG
    AAGAAGCTCG CCACTTCTCG AAAACAGAGC CTTGACCTGC CCCACCCCGA GCTAAAGAAG
    GAATTGCCGC CCAGGGAAGT CAGCTCTCTG GAGGTAGTTG GGACCAGGAG TGTGCTGTCT
    GGAAAGGGGG CGCTGCCAGG GAAAGGGGTG TTGCAGGCTG CTCCCTCACG GGCCTTAGGG
    ACCCTCCGCC AGGACAGGGC TGAACGGCGA GAGTCGCTGC AGAAGCAGGA AGCCATCCGG
    GAGGTTGACT CTTCAGAAGA TGATACTGAT GAGGGGCCTG AGAACAGCCA AGGTGTTCAG
    GAGCCAAGCT TGGTACCCCA CCGAGAAGGG GGCCAGGATC CACCCCTCAG AGGAGCAGGA
    GAGGGTGAGG AAGAGGATGC CTTCTTGCCC AGGGATCCCA GGGGACAGGG CCCAGTGGTC
    ACAGGTCTAC TGACAGGGGT CACACTGGGA TCTCCCAGAA TGGAAGGCCC TGGTGTCCCC
    CAGAAGAAGC TTGGAATCCT ACAGGCTTTT GAAGAGGGTT CCAGCTCCTC GTCCTCAGCC
    CCCAACCTGG GGAAGGCTGG CCCCACAGAC CCCATCCCCG CTGAAGGCTG CTGGAAGGCC
    CAGCACCTCC ACACCCAGGC ACTGACAGCA CTTTGTCCGA GCTCATCAGG ACGCATCCCT
    GCCAGTTGCT CCACTGCCTC CACCCCTGGA GAGCTGGGCC CATGGTCCTG GAAATTCCTC
    TTCAAGGGCC CTGACAGGGC ATACCCAACC GGAAAGGCAG CAATGGAAGA TGGGCCAGCC
    AGCTCCCAAG ATTTGGAAAC TACAACTCCA GGCCACCCTG CAAACCTGTC CCCCAGACAG
    GAGGGGAAGT CATGGCCACC CAGTGCCCCT GGGCTGGCCC ATCCACCTTG TGAGGTTCCC
    AGCCAGAGCT GGCTATGGGA GTCTGAGTGT GCACAGAGAG AGAAAGAGGA GCCAGCGCTG
    GGCATCACCA AAGTGCCTGA TGCCTCAGGT GACAGGAGGC AGGACGTTCC ATGCAAGAGC
    TGCCCCTTCA CCCCAGAGCC TGGGCCCAGC CTGCTCCAGA AGGGCCAAGA CCCAGGGGGC
    CCTCAGGAGC ATCAGGACTT GGCATTGTTA CCTGATGAGC TTTTAAAGCA AACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005204940.1
    CDS244..5400
    Translation

    Target ORF information:

    RefSeq Version XM_005204883.4
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204883.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
    CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG
    TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT
    TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGTCCCT GTTCCAGCCG CTCACTGCAG
    TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA
    CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC
    CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGCCATGGC
    CACAGAGCAG ACAGGACTGA TGGACGGCGC TGGTCTTTGG CCTCATTGCC TTCTTCAGGC
    TATGGAACCA ACACCCCTAG TTCTACCGTG TCATCGTCGT GCTCCTCTCA GGAGAAGCTG
    CACCAGTTGC CTTTCCAGCC CACGGCCGAT GAGCTGCACT TCTTGACGAA GCATTTCAGC
    ACAGACAGTG TGCCTGATGA GGAGGGGCGG CAGTCCCCCG CCCTGCGGCC CCGCTCCCGC
    AGCCTCAGTC CTGGACGGTC ACCAGTTTCC TTTGACAGTG AAATAATAAT GATGAATCAT
    GTATACAAGG AAAGATTCCC CAAGGCCACT GCACAAATGG AAGAGCGTCT GGCCGAGTTC
    ATCTCCTCCA ATACTCCAGA CAACGTGTTG CCCTTGGCTG ATGGAACCTT GAGCTTTATT
    CATCATCAGG TGATAGAGAT GGCCCGAGAC TGCCTGGACA AATCCCGGAG TGGCCTCATT
    ACCTCACACT ACTTCTACGA ACTTCAAGAG AATTTAGAGA AGCTTCTGCA AGATGCTCAT
    GAGCGCTCAG AGAGTTCAGA AGTGGCTTTT GTGATGCAGC TGGTGAAAAA GCTGATGATT
    GTCATTGCCC GCCCAGCTCG TCTCCTGGAA TGCCTGGAGT TTGACCCTGA AGAGTTCTAC
    CACCTGTTAG AAGCAGCTGA GGGCCACGCC AAAGAGGGAC AAGGGATTAA ATGTGACATT
    CCCCGCTACA TCGTTAGCCA ACTGGGCCTC ACACGGGATC CCCTAGAGGA AATGGCCCAG
    TTGAGCAGCT ATGACAGTCC TGATACTCCG GAGACAGATG ATTCAGTCGA GGGCCGTGGG
    GCATCTCTGA CATCGAAAAA GACACCCTCT GAGGAGGACT TCGAAACCAT TAAGCTCATC
    AGCAATGGCG CCTATGGGGC CGTGTTCCTG GTGCGGCACA AGTCCACCCG GCAGCGCTTT
    GCCATGAAGA AGATCAACAA GCAGAACCTG ATCCTGCGGA ACCAGATCCA GCAGGCCTTC
    GTGGAGCGGG ACATCCTGAC TTTTGCTGAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC
    TTTGAAACCA AGCGCCACCT GTGCATGGTG ATGGAGTATG TGGAAGGAGG AGACTGTGCC
    ACGCTGCTGA AGAACATCGG GGCCCTGCCT GTGGACATGG TGCGCCTGTA CTTTGCAGAA
    ACTGTGCTAG CCCTGGAGTA CTTACACAAT TACGGCATCG TGCACCGTGA CCTCAAGCCT
    GACAACCTGC TGATTACATC CATGGGTCAC ATCAAGCTCA CTGATTTTGG CCTTTCCAAA
    ATTGGCCTTA TGAGTCTGAC CACAAACTTG TACGAGGGCC ACATTGAAAA GGATGCACGG
    GAGTTCCTGG ACAAGCAGGT ATGCGGGACC CCGGAGTACA TTGCACCTGA GGTGATCCTG
    CGGCAGGGCT ACGGGAAGCC AGTGGACTGG TGGGCTATGG GCATCATCCT GTACGAGTTC
    CTGGTGGGCT GCGTCCCTTT CTTTGGAGAC ACTCCGGAGG AGCTCTTTGG GCAAGTGATC
    AGTGATGAGA TTGTGTGGCC TGAGGGGGAC GATGCACTGC CGCCGGACGC CCAAGATCTC
    ACCTCAAAAC TGCTCCACCA GAACCCATTG GAGAGACTGG GCACAGGCAG TGCCTATGAG
    GTGAAGCAGC ACCCATTCTT CACTGGTCTG GACTGGACGG GACTTCTTCG CCAGAAGGCT
    GAATTTATTC CTCAGCTGGA GTCAGAGGAT GATACCAGCT ACTTTGACAC CCGCTCAGAG
    CGATACCACC ACGTGGACTC GGAGGATGAG GAGGAAGTGA GTGAGGATGG CTGCCTTGAG
    ATTCGCCAGT TCTCGTCCTG TTCTCCAAGG TTCAGCAAGG TGTACAGCAG CATGGAGCGG
    CTCTCGCTGC TCGAGGAGCG CCGGACGCCA CCCCCCACCA AGCGCAGCCT GAGTGAGGAA
    AAGGAGGACC GCACGGATGG CCTGGCGGGG CTGAGGGGCC GAGAGCGAAG CTGGGTGATC
    GGCTCCCCTG AGATATTACG GAAGCGGCTG TCGGTGTCCG AGTCGTCCCA CACGGAAAGC
    GATTCCAGCC CTCCACTCAC CGTGCGGCGC CACTGCTCGG GCCTCCTGGA CGTGCCTCGC
    TTCCCTGAGA GCACTGAGGA GGCTGTTGGC GCTCCCAGGA GGCAGCAGCA GGAGGGTGCA
    TGGCTTCTGA CCCCGTCTGG AGAGGGGGCG TCTGGGCCTG CCCTGGAACG GCCGGCCGAG
    CGGCGGCTGA AGCTGAATGA GGAACCTGCT GGCCAGAGCA GCGCCCCCAG CCCAGCCATG
    GAGACCCGAG GAGGCCGCGG AACCCCACAG CTGGCAGAAG GAGCCACAGC CAGGGCCATC
    AGCGACCTGG CGGTGCGCAG GGCCCGCCAC CGGCTGCTCT CTGGGGACTC CATAGAAAAG
    CGCACCGCTC GCCCCATCAA CAAAGTGATC AAGTCCGCCT CAGCCACAGC CCTGTCCCTT
    CTCATTCCTG CTGAGCACCA CTCCTGCTCT CCACTGGCCA GCCCCATGTC CCCACATTCC
    CAGTCATCCA ACCCATCATC CAGGGACTCT TCTCCAAGCA GGGACTTCCT GCCAGCCCTC
    AGCAGCTCAC GGCCCCCCAT TGTCATCCAC CGAGCCGGCA AGAAGTATGG CTTCACCCTA
    CGAGCCATCC GTGTCTACAT GGGTGACTCA GATGTCTACA CCGTGCACCA CATGGTGTGG
    CACGTGGAGG ACGGAGGTCC AGCCAGTGAG GCAGGGCTGC GTCAGGGGGA CCTCATCACG
    CACGTCAACG GGGAGCCTGT GCACGGGCTG GTGCACACGG AGGTGGTGGA GCTGATCCTG
    AAGAGTGGAA ACAAGGTGTC TATTTCAACA ACTCCCCTGG AGAACACCTC CATTAAGGTG
    GGGCCCGCTC GGAAGGGCAG CTACAAGGCC AAGATGGCCC GAAGGAGCAA GCGGAGCCGG
    GGCAAGGATG GACAAGAAAG CAGAAAAAGG AGCTCCCTGT TCCGGAAGAT CACCAAGCAG
    GCATCCCTGC TCCACACCAG CCGCAGCCTT TCTTCCCTTA ACCGCTCCCT GTCGTCCGGC
    GAGAGTGGGC CAGGCTCGCC CACACACAGC CACAGCCTTT CTCCCCGCTC TCCCACGCAG
    GGCTACCGGG TGACCCCTGA AGCCGTGCAC TCAGTGGGGG GGAATTCATC ACAGAGCAGC
    TCCCCCAGCT CCAGCGTGCC CAGTTCTCCT GCCGGCTCTG GGCACACACG GCCGAGCTCC
    CTCCACGGTC TGGCGCCCAA GCTCCAACGC CAGTACCGCT CTCCACGGCG CAAGTCAGCA
    GGCAGCATCC CGCTGTCACC ACTGGCCCAC ACCCCTTCAC CACCACCAGC GGCTTCGCCT
    CAGCGTTCCC CATCACCTCT GTCTGGCCAC GGGGCCCAGG CCTTCCCTAC CAAGCTCCAC
    TTGTCGCCTC CCCTAGGCCG GCAACTCTCA CGGCCCAAGA GCGCGGAGCC ACCCCGCTCA
    CCACTACTTA AGAGGGTGCA GTCAGCTGAA AAACTGGCAG CTGCACTGGC TGCTTCTGAG
    AAGAAGCTCG CCACTTCTCG AAAACAGAGC CTTGACCTGC CCCACCCCGA GCTAAAGAAG
    GAATTGCCGC CCAGGGAAGT CAGCTCTCTG GAGGTAGTTG GGACCAGGAG TGTGCTGTCT
    GGAAAGGGGG CGCTGCCAGG GAAAGGGGTG TTGCAGGCTG CTCCCTCACG GGCCTTAGGG
    ACCCTCCGCC AGGACAGGGC TGAACGGCGA GAGTCGCTGC AGAAGCAGGA AGCCATCCGG
    GAGGTTGACT CTTCAGAAGA TGATACTGAT GAGGGGCCTG AGAACAGCCA AGGTGTTCAG
    GAGCCAAGCT TGGTACCCCA CCGAGAAGGG GGCCAGGATC CACCCCTCAG AGGAGCAGGA
    GAGGGTGAGG AAGAGGATGC CTTCTTGCCC AGGGATCCCA GGGGACAGGG CCCAGTGGTC
    ACAGGTCTAC TGACAGGGGT CACACTGGGA TCTCCCAGAA TGGAAGGCCC TGGTGTCCCC
    CAGAAGAAGC TTGGAATCCT ACAGGCTTTT GAAGAGGGTT CCAGCTCCTC GTCCTCAGCC
    CCCAACCTGG GGAAGGCTGG CCCCACAGAC CCCATCCCCG CTGAAGGCTG CTGGAAGGCC
    CAGCACCTCC ACACCCAGGC ACTGACAGCA CTTTGTCCGA GCTCATCAGG ACGCATCCCT
    GCCAGTTGCT CCACTGCCTC CACCCCTGGA GAGCTGGGCC CATGGTCCTG GAAATTCCTC
    TTCAAGGGCC CTGACAGGGC ATACCCAACC GGAAAGGCAG CAATGGAAGA TGGGCCAGCC
    AGCTCCCAAG ATTTGGAAAC TACAACTCCA GGCCACCCTG CAAACCTGTC CCCCAGACAG
    GAGGGGAAGT CATGGCCACC CAGTGCCCCT GGGCTGGCCC ATCCACCTTG TGAGGTTCCC
    AGCCAGAGCT GGCTATGGGA GTCTGAGTGT GCACAGAGAG AGAAAGAGGA GCCAGCGCTG
    GGCATCACCA AAGTGCCTGA TGCCTCAGGT GACAGGAGGC AGGACGTTCC ATGCAAGAGC
    TGCCCCTTCA CCCCAGAGCC TGGGCCCAGC CTGCTCCAGA AGGGCCAAGA CCCAGGGGGC
    CCTCAGGAGC ATCAGGACTT GGCATTGTTA CCTGATGAGC TTTTAAAGCA AACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233323
    Clone ID Related Accession (Same CDS sequence) XM_005204884.4
    Accession Version XM_005204884.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5136bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204884.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
    CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG
    TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT
    TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGTCCCT GTTCCAGCCG CTCACTGCAG
    TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA
    CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC
    CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGGACTGAT
    GGACGGCGCT GGTCTTTGGC CTCATTGCCT TCTTCAGGCT ATGGAACCAA CACCCCTAGT
    TCTACCGTGT CATCGTCGTG CTCCTCTCAG GAGAAGCTGC ACCAGTTGCC TTTCCAGCCC
    ACGGCCGATG AGCTGCACTT CTTGACGAAG CATTTCAGCA CAGACAGTGT GCCTGATGAG
    GAGGGGCGGC AGTCCCCCGC CCTGCGGCCC CGCTCCCGCA GCCTCAGTCC TGGACGGTCA
    CCAGTTTCCT TTGACAGTGA AATAATAATG ATGAATCATG TATACAAGGA AAGATTCCCC
    AAGGCCACTG CACAAATGGA AGAGCGTCTG GCCGAGTTCA TCTCCTCCAA TACTCCAGAC
    AACGTGTTGC CCTTGGCTGA TGGAACCTTG AGCTTTATTC ATCATCAGGT GATAGAGATG
    GCCCGAGACT GCCTGGACAA ATCCCGGAGT GGCCTCATTA CCTCACACTA CTTCTACGAA
    CTTCAAGAGA ATTTAGAGAA GCTTCTGCAA GATGCTCATG AGCGCTCAGA GAGTTCAGAA
    GTGGCTTTTG TGATGCAGCT GGTGAAAAAG CTGATGATTG TCATTGCCCG CCCAGCTCGT
    CTCCTGGAAT GCCTGGAGTT TGACCCTGAA GAGTTCTACC ACCTGTTAGA AGCAGCTGAG
    GGCCACGCCA AAGAGGGACA AGGGATTAAA TGTGACATTC CCCGCTACAT CGTTAGCCAA
    CTGGGCCTCA CACGGGATCC CCTAGAGGAA ATGGCCCAGT TGAGCAGCTA TGACAGTCCT
    GATACTCCGG AGACAGATGA TTCAGTCGAG GGCCGTGGGG CATCTCTGAC ATCGAAAAAG
    ACACCCTCTG AGGAGGACTT CGAAACCATT AAGCTCATCA GCAATGGCGC CTATGGGGCC
    GTGTTCCTGG TGCGGCACAA GTCCACCCGG CAGCGCTTTG CCATGAAGAA GATCAACAAG
    CAGAACCTGA TCCTGCGGAA CCAGATCCAG CAGGCCTTCG TGGAGCGGGA CATCCTGACT
    TTTGCTGAGA ACCCCTTTGT GGTCAGCATG TTCTGCTCCT TTGAAACCAA GCGCCACCTG
    TGCATGGTGA TGGAGTATGT GGAAGGAGGA GACTGTGCCA CGCTGCTGAA GAACATCGGG
    GCCCTGCCTG TGGACATGGT GCGCCTGTAC TTTGCAGAAA CTGTGCTAGC CCTGGAGTAC
    TTACACAATT ACGGCATCGT GCACCGTGAC CTCAAGCCTG ACAACCTGCT GATTACATCC
    ATGGGTCACA TCAAGCTCAC TGATTTTGGC CTTTCCAAAA TTGGCCTTAT GAGTCTGACC
    ACAAACTTGT ACGAGGGCCA CATTGAAAAG GATGCACGGG AGTTCCTGGA CAAGCAGGTA
    TGCGGGACCC CGGAGTACAT TGCACCTGAG GTGATCCTGC GGCAGGGCTA CGGGAAGCCA
    GTGGACTGGT GGGCTATGGG CATCATCCTG TACGAGTTCC TGGTGGGCTG CGTCCCTTTC
    TTTGGAGACA CTCCGGAGGA GCTCTTTGGG CAAGTGATCA GTGATGAGAT TGTGTGGCCT
    GAGGGGGACG ATGCACTGCC GCCGGACGCC CAAGATCTCA CCTCAAAACT GCTCCACCAG
    AACCCATTGG AGAGACTGGG CACAGGCAGT GCCTATGAGG TGAAGCAGCA CCCATTCTTC
    ACTGGTCTGG ACTGGACGGG ACTTCTTCGC CAGAAGGCTG AATTTATTCC TCAGCTGGAG
    TCAGAGGATG ATACCAGCTA CTTTGACACC CGCTCAGAGC GATACCACCA CGTGGACTCG
    GAGGATGAGG AGGAAGTGAG TGAGGATGGC TGCCTTGAGA TTCGCCAGTT CTCGTCCTGT
    TCTCCAAGGT TCAGCAAGGT GTACAGCAGC ATGGAGCGGC TCTCGCTGCT CGAGGAGCGC
    CGGACGCCAC CCCCCACCAA GCGCAGCCTG AGTGAGGAAA AGGAGGACCG CACGGATGGC
    CTGGCGGGGC TGAGGGGCCG AGAGCGAAGC TGGGTGATCG GCTCCCCTGA GATATTACGG
    AAGCGGCTGT CGGTGTCCGA GTCGTCCCAC ACGGAAAGCG ATTCCAGCCC TCCACTCACC
    GTGCGGCGCC ACTGCTCGGG CCTCCTGGAC GTGCCTCGCT TCCCTGAGAG CACTGAGGAG
    GCTGTTGGCG CTCCCAGGAG GCAGCAGCAG GAGGGTGCAT GGCTTCTGAC CCCGTCTGGA
    GAGGGGGCGT CTGGGCCTGC CCTGGAACGG CCGGCCGAGC GGCGGCTGAA GCTGAATGAG
    GAACCTGCTG GCCAGAGCAG CGCCCCCAGC CCAGCCATGG AGACCCGAGG AGGCCGCGGA
    ACCCCACAGC TGGCAGAAGG AGCCACAGCC AGGGCCATCA GCGACCTGGC GGTGCGCAGG
    GCCCGCCACC GGCTGCTCTC TGGGGACTCC ATAGAAAAGC GCACCGCTCG CCCCATCAAC
    AAAGTGATCA AGTCCGCCTC AGCCACAGCC CTGTCCCTTC TCATTCCTGC TGAGCACCAC
    TCCTGCTCTC CACTGGCCAG CCCCATGTCC CCACATTCCC AGTCATCCAA CCCATCATCC
    AGGGACTCTT CTCCAAGCAG GGACTTCCTG CCAGCCCTCA GCAGCTCACG GCCCCCCATT
    GTCATCCACC GAGCCGGCAA GAAGTATGGC TTCACCCTAC GAGCCATCCG TGTCTACATG
    GGTGACTCAG ATGTCTACAC CGTGCACCAC ATGGTGTGGC ACGTGGAGGA CGGAGGTCCA
    GCCAGTGAGG CAGGGCTGCG TCAGGGGGAC CTCATCACGC ACGTCAACGG GGAGCCTGTG
    CACGGGCTGG TGCACACGGA GGTGGTGGAG CTGATCCTGA AGAGTGGAAA CAAGGTGTCT
    ATTTCAACAA CTCCCCTGGA GAACACCTCC ATTAAGGTGG GGCCCGCTCG GAAGGGCAGC
    TACAAGGCCA AGATGGCCCG AAGGAGCAAG CGGAGCCGGG GCAAGGATGG ACAAGAAAGC
    AGAAAAAGGA GCTCCCTGTT CCGGAAGATC ACCAAGCAGG CATCCCTGCT CCACACCAGC
    CGCAGCCTTT CTTCCCTTAA CCGCTCCCTG TCGTCCGGCG AGAGTGGGCC AGGCTCGCCC
    ACACACAGCC ACAGCCTTTC TCCCCGCTCT CCCACGCAGG GCTACCGGGT GACCCCTGAA
    GCCGTGCACT CAGTGGGGGG GAATTCATCA CAGAGCAGCT CCCCCAGCTC CAGCGTGCCC
    AGTTCTCCTG CCGGCTCTGG GCACACACGG CCGAGCTCCC TCCACGGTCT GGCGCCCAAG
    CTCCAACGCC AGTACCGCTC TCCACGGCGC AAGTCAGCAG GCAGCATCCC GCTGTCACCA
    CTGGCCCACA CCCCTTCACC ACCACCAGCG GCTTCGCCTC AGCGTTCCCC ATCACCTCTG
    TCTGGCCACG GGGCCCAGGC CTTCCCTACC AAGCTCCACT TGTCGCCTCC CCTAGGCCGG
    CAACTCTCAC GGCCCAAGAG CGCGGAGCCA CCCCGCTCAC CACTACTTAA GAGGGTGCAG
    TCAGCTGAAA AACTGGCAGC TGCACTGGCT GCTTCTGAGA AGAAGCTCGC CACTTCTCGA
    AAACAGAGCC TTGACCTGCC CCACCCCGAG CTAAAGAAGG AATTGCCGCC CAGGGAAGTC
    AGCTCTCTGG AGGTAGTTGG GACCAGGAGT GTGCTGTCTG GAAAGGGGGC GCTGCCAGGG
    AAAGGGGTGT TGCAGGCTGC TCCCTCACGG GCCTTAGGGA CCCTCCGCCA GGACAGGGCT
    GAACGGCGAG AGTCGCTGCA GAAGCAGGAA GCCATCCGGG AGGTTGACTC TTCAGAAGAT
    GATACTGATG AGGGGCCTGA GAACAGCCAA GGTGTTCAGG AGCCAAGCTT GGTACCCCAC
    CGAGAAGGGG GCCAGGATCC ACCCCTCAGA GGAGCAGGAG AGGGTGAGGA AGAGGATGCC
    TTCTTGCCCA GGGATCCCAG GGGACAGGGC CCAGTGGTCA CAGGTCTACT GACAGGGGTC
    ACACTGGGAT CTCCCAGAAT GGAAGGCCCT GGTGTCCCCC AGAAGAAGCT TGGAATCCTA
    CAGGCTTTTG AAGAGGGTTC CAGCTCCTCG TCCTCAGCCC CCAACCTGGG GAAGGCTGGC
    CCCACAGACC CCATCCCCGC TGAAGGCTGC TGGAAGGCCC AGCACCTCCA CACCCAGGCA
    CTGACAGCAC TTTGTCCGAG CTCATCAGGA CGCATCCCTG CCAGTTGCTC CACTGCCTCC
    ACCCCTGGAG AGCTGGGCCC ATGGTCCTGG AAATTCCTCT TCAAGGGCCC TGACAGGGCA
    TACCCAACCG GAAAGGCAGC AATGGAAGAT GGGCCAGCCA GCTCCCAAGA TTTGGAAACT
    ACAACTCCAG GCCACCCTGC AAACCTGTCC CCCAGACAGG AGGGGAAGTC ATGGCCACCC
    AGTGCCCCTG GGCTGGCCCA TCCACCTTGT GAGGTTCCCA GCCAGAGCTG GCTATGGGAG
    TCTGAGTGTG CACAGAGAGA GAAAGAGGAG CCAGCGCTGG GCATCACCAA AGTGCCTGAT
    GCCTCAGGTG ACAGGAGGCA GGACGTTCCA TGCAAGAGCT GCCCCTTCAC CCCAGAGCCT
    GGGCCCAGCC TGCTCCAGAA GGGCCAAGAC CCAGGGGGCC CTCAGGAGCA TCAGGACTTG
    GCATTGTTAC CTGATGAGCT TTTAAAGCAA ACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005204941.1
    CDS244..5379
    Translation

    Target ORF information:

    RefSeq Version XM_005204884.4
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204884.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
    CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG
    TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT
    TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGTCCCT GTTCCAGCCG CTCACTGCAG
    TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA
    CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC
    CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGGACTGAT
    GGACGGCGCT GGTCTTTGGC CTCATTGCCT TCTTCAGGCT ATGGAACCAA CACCCCTAGT
    TCTACCGTGT CATCGTCGTG CTCCTCTCAG GAGAAGCTGC ACCAGTTGCC TTTCCAGCCC
    ACGGCCGATG AGCTGCACTT CTTGACGAAG CATTTCAGCA CAGACAGTGT GCCTGATGAG
    GAGGGGCGGC AGTCCCCCGC CCTGCGGCCC CGCTCCCGCA GCCTCAGTCC TGGACGGTCA
    CCAGTTTCCT TTGACAGTGA AATAATAATG ATGAATCATG TATACAAGGA AAGATTCCCC
    AAGGCCACTG CACAAATGGA AGAGCGTCTG GCCGAGTTCA TCTCCTCCAA TACTCCAGAC
    AACGTGTTGC CCTTGGCTGA TGGAACCTTG AGCTTTATTC ATCATCAGGT GATAGAGATG
    GCCCGAGACT GCCTGGACAA ATCCCGGAGT GGCCTCATTA CCTCACACTA CTTCTACGAA
    CTTCAAGAGA ATTTAGAGAA GCTTCTGCAA GATGCTCATG AGCGCTCAGA GAGTTCAGAA
    GTGGCTTTTG TGATGCAGCT GGTGAAAAAG CTGATGATTG TCATTGCCCG CCCAGCTCGT
    CTCCTGGAAT GCCTGGAGTT TGACCCTGAA GAGTTCTACC ACCTGTTAGA AGCAGCTGAG
    GGCCACGCCA AAGAGGGACA AGGGATTAAA TGTGACATTC CCCGCTACAT CGTTAGCCAA
    CTGGGCCTCA CACGGGATCC CCTAGAGGAA ATGGCCCAGT TGAGCAGCTA TGACAGTCCT
    GATACTCCGG AGACAGATGA TTCAGTCGAG GGCCGTGGGG CATCTCTGAC ATCGAAAAAG
    ACACCCTCTG AGGAGGACTT CGAAACCATT AAGCTCATCA GCAATGGCGC CTATGGGGCC
    GTGTTCCTGG TGCGGCACAA GTCCACCCGG CAGCGCTTTG CCATGAAGAA GATCAACAAG
    CAGAACCTGA TCCTGCGGAA CCAGATCCAG CAGGCCTTCG TGGAGCGGGA CATCCTGACT
    TTTGCTGAGA ACCCCTTTGT GGTCAGCATG TTCTGCTCCT TTGAAACCAA GCGCCACCTG
    TGCATGGTGA TGGAGTATGT GGAAGGAGGA GACTGTGCCA CGCTGCTGAA GAACATCGGG
    GCCCTGCCTG TGGACATGGT GCGCCTGTAC TTTGCAGAAA CTGTGCTAGC CCTGGAGTAC
    TTACACAATT ACGGCATCGT GCACCGTGAC CTCAAGCCTG ACAACCTGCT GATTACATCC
    ATGGGTCACA TCAAGCTCAC TGATTTTGGC CTTTCCAAAA TTGGCCTTAT GAGTCTGACC
    ACAAACTTGT ACGAGGGCCA CATTGAAAAG GATGCACGGG AGTTCCTGGA CAAGCAGGTA
    TGCGGGACCC CGGAGTACAT TGCACCTGAG GTGATCCTGC GGCAGGGCTA CGGGAAGCCA
    GTGGACTGGT GGGCTATGGG CATCATCCTG TACGAGTTCC TGGTGGGCTG CGTCCCTTTC
    TTTGGAGACA CTCCGGAGGA GCTCTTTGGG CAAGTGATCA GTGATGAGAT TGTGTGGCCT
    GAGGGGGACG ATGCACTGCC GCCGGACGCC CAAGATCTCA CCTCAAAACT GCTCCACCAG
    AACCCATTGG AGAGACTGGG CACAGGCAGT GCCTATGAGG TGAAGCAGCA CCCATTCTTC
    ACTGGTCTGG ACTGGACGGG ACTTCTTCGC CAGAAGGCTG AATTTATTCC TCAGCTGGAG
    TCAGAGGATG ATACCAGCTA CTTTGACACC CGCTCAGAGC GATACCACCA CGTGGACTCG
    GAGGATGAGG AGGAAGTGAG TGAGGATGGC TGCCTTGAGA TTCGCCAGTT CTCGTCCTGT
    TCTCCAAGGT TCAGCAAGGT GTACAGCAGC ATGGAGCGGC TCTCGCTGCT CGAGGAGCGC
    CGGACGCCAC CCCCCACCAA GCGCAGCCTG AGTGAGGAAA AGGAGGACCG CACGGATGGC
    CTGGCGGGGC TGAGGGGCCG AGAGCGAAGC TGGGTGATCG GCTCCCCTGA GATATTACGG
    AAGCGGCTGT CGGTGTCCGA GTCGTCCCAC ACGGAAAGCG ATTCCAGCCC TCCACTCACC
    GTGCGGCGCC ACTGCTCGGG CCTCCTGGAC GTGCCTCGCT TCCCTGAGAG CACTGAGGAG
    GCTGTTGGCG CTCCCAGGAG GCAGCAGCAG GAGGGTGCAT GGCTTCTGAC CCCGTCTGGA
    GAGGGGGCGT CTGGGCCTGC CCTGGAACGG CCGGCCGAGC GGCGGCTGAA GCTGAATGAG
    GAACCTGCTG GCCAGAGCAG CGCCCCCAGC CCAGCCATGG AGACCCGAGG AGGCCGCGGA
    ACCCCACAGC TGGCAGAAGG AGCCACAGCC AGGGCCATCA GCGACCTGGC GGTGCGCAGG
    GCCCGCCACC GGCTGCTCTC TGGGGACTCC ATAGAAAAGC GCACCGCTCG CCCCATCAAC
    AAAGTGATCA AGTCCGCCTC AGCCACAGCC CTGTCCCTTC TCATTCCTGC TGAGCACCAC
    TCCTGCTCTC CACTGGCCAG CCCCATGTCC CCACATTCCC AGTCATCCAA CCCATCATCC
    AGGGACTCTT CTCCAAGCAG GGACTTCCTG CCAGCCCTCA GCAGCTCACG GCCCCCCATT
    GTCATCCACC GAGCCGGCAA GAAGTATGGC TTCACCCTAC GAGCCATCCG TGTCTACATG
    GGTGACTCAG ATGTCTACAC CGTGCACCAC ATGGTGTGGC ACGTGGAGGA CGGAGGTCCA
    GCCAGTGAGG CAGGGCTGCG TCAGGGGGAC CTCATCACGC ACGTCAACGG GGAGCCTGTG
    CACGGGCTGG TGCACACGGA GGTGGTGGAG CTGATCCTGA AGAGTGGAAA CAAGGTGTCT
    ATTTCAACAA CTCCCCTGGA GAACACCTCC ATTAAGGTGG GGCCCGCTCG GAAGGGCAGC
    TACAAGGCCA AGATGGCCCG AAGGAGCAAG CGGAGCCGGG GCAAGGATGG ACAAGAAAGC
    AGAAAAAGGA GCTCCCTGTT CCGGAAGATC ACCAAGCAGG CATCCCTGCT CCACACCAGC
    CGCAGCCTTT CTTCCCTTAA CCGCTCCCTG TCGTCCGGCG AGAGTGGGCC AGGCTCGCCC
    ACACACAGCC ACAGCCTTTC TCCCCGCTCT CCCACGCAGG GCTACCGGGT GACCCCTGAA
    GCCGTGCACT CAGTGGGGGG GAATTCATCA CAGAGCAGCT CCCCCAGCTC CAGCGTGCCC
    AGTTCTCCTG CCGGCTCTGG GCACACACGG CCGAGCTCCC TCCACGGTCT GGCGCCCAAG
    CTCCAACGCC AGTACCGCTC TCCACGGCGC AAGTCAGCAG GCAGCATCCC GCTGTCACCA
    CTGGCCCACA CCCCTTCACC ACCACCAGCG GCTTCGCCTC AGCGTTCCCC ATCACCTCTG
    TCTGGCCACG GGGCCCAGGC CTTCCCTACC AAGCTCCACT TGTCGCCTCC CCTAGGCCGG
    CAACTCTCAC GGCCCAAGAG CGCGGAGCCA CCCCGCTCAC CACTACTTAA GAGGGTGCAG
    TCAGCTGAAA AACTGGCAGC TGCACTGGCT GCTTCTGAGA AGAAGCTCGC CACTTCTCGA
    AAACAGAGCC TTGACCTGCC CCACCCCGAG CTAAAGAAGG AATTGCCGCC CAGGGAAGTC
    AGCTCTCTGG AGGTAGTTGG GACCAGGAGT GTGCTGTCTG GAAAGGGGGC GCTGCCAGGG
    AAAGGGGTGT TGCAGGCTGC TCCCTCACGG GCCTTAGGGA CCCTCCGCCA GGACAGGGCT
    GAACGGCGAG AGTCGCTGCA GAAGCAGGAA GCCATCCGGG AGGTTGACTC TTCAGAAGAT
    GATACTGATG AGGGGCCTGA GAACAGCCAA GGTGTTCAGG AGCCAAGCTT GGTACCCCAC
    CGAGAAGGGG GCCAGGATCC ACCCCTCAGA GGAGCAGGAG AGGGTGAGGA AGAGGATGCC
    TTCTTGCCCA GGGATCCCAG GGGACAGGGC CCAGTGGTCA CAGGTCTACT GACAGGGGTC
    ACACTGGGAT CTCCCAGAAT GGAAGGCCCT GGTGTCCCCC AGAAGAAGCT TGGAATCCTA
    CAGGCTTTTG AAGAGGGTTC CAGCTCCTCG TCCTCAGCCC CCAACCTGGG GAAGGCTGGC
    CCCACAGACC CCATCCCCGC TGAAGGCTGC TGGAAGGCCC AGCACCTCCA CACCCAGGCA
    CTGACAGCAC TTTGTCCGAG CTCATCAGGA CGCATCCCTG CCAGTTGCTC CACTGCCTCC
    ACCCCTGGAG AGCTGGGCCC ATGGTCCTGG AAATTCCTCT TCAAGGGCCC TGACAGGGCA
    TACCCAACCG GAAAGGCAGC AATGGAAGAT GGGCCAGCCA GCTCCCAAGA TTTGGAAACT
    ACAACTCCAG GCCACCCTGC AAACCTGTCC CCCAGACAGG AGGGGAAGTC ATGGCCACCC
    AGTGCCCCTG GGCTGGCCCA TCCACCTTGT GAGGTTCCCA GCCAGAGCTG GCTATGGGAG
    TCTGAGTGTG CACAGAGAGA GAAAGAGGAG CCAGCGCTGG GCATCACCAA AGTGCCTGAT
    GCCTCAGGTG ACAGGAGGCA GGACGTTCCA TGCAAGAGCT GCCCCTTCAC CCCAGAGCCT
    GGGCCCAGCC TGCTCCAGAA GGGCCAAGAC CCAGGGGGCC CTCAGGAGCA TCAGGACTTG
    GCATTGTTAC CTGATGAGCT TTTAAAGCAA ACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53214
    Clone ID Related Accession (Same CDS sequence) XM_024990152.1
    Accession Version XM_024990152.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5250bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGATTC TCAAGGATGT GGTAACTGGA CGTAGTCCGC TGCTCTTCAG GAAGCTCAGT 
    AATCCTGACA TATTTTCATC CACTGGAAAA GCTAAACTTC AACGACAACT TAGTCAGGAT
    GACTGTAAGT TACGGAGAGG AAGCCTGGCC AGTTCTTTGT CAGGCAAGCA GCTGCTCCCC
    TTGTCCGGCA GTGCACACAG CAGCGTGGGA CAGGTGACTT GGCAGTCTTC AGGAGAAGCG
    TCCAACCTGG TGCGGATGAG GAATCAGTCC CTCGGACAGT CTGCGCCTTC TCTTACCGCT
    GGCTTGAAGG AGCTGAGCCT TCCAAGAAGA GGCAGCTTCT GTCGGACAAG TAACCGCAAG
    AGTTTGATTG TGACCTCTAG TACGTCACCT ACACTGCCAC GGCCACACTC ACCACTCCAT
    GGCCACACAG GTAACAGTCC TTTGGATAGT CCCCGGAATT TCTCTCCAAA TGCACCTGCT
    CACTTTTCCT TTGTTCCTGC CCGTAGCCAT GGCCACAGAG CAGACAGGAC TGATGGACGG
    CGCTGGTCTT TGGCCTCATT GCCTTCTTCA GGCTATGGAA CCAACACCCC TAGTTCTACC
    GTGTCATCGT CGTGCTCCTC TCAGGAGAAG CTGCACCAGT TGCCTTTCCA GCCCACGGCC
    GATGAGCTGC ACTTCTTGAC GAAGCATTTC AGCACAGACA GTGTGCCTGA TGAGGAGGGG
    CGGCAGTCCC CCGCCCTGCG GCCCCGCTCC CGCAGCCTCA GTCCTGGACG GTCACCAGTT
    TCCTTTGACA GTGAAATAAT AATGATGAAT CATGTATACA AGGAAAGATT CCCCAAGGCC
    ACTGCACAAA TGGAAGAGCG TCTGGCCGAG TTCATCTCCT CCAATACTCC AGACAACGTG
    TTGCCCTTGG CTGATGGAAC CTTGAGCTTT ATTCATCATC AGGTGATAGA GATGGCCCGA
    GACTGCCTGG ACAAATCCCG GAGTGGCCTC ATTACCTCAC ACTACTTCTA CGAACTTCAA
    GAGAATTTAG AGAAGCTTCT GCAAGATGCT CATGAGCGCT CAGAGAGTTC AGAAGTGGCT
    TTTGTGATGC AGCTGGTGAA AAAGCTGATG ATTGTCATTG CCCGCCCAGC TCGTCTCCTG
    GAATGCCTGG AGTTTGACCC TGAAGAGTTC TACCACCTGT TAGAAGCAGC TGAGGGCCAC
    GCCAAAGAGG GACAAGGGAT TAAATGTGAC ATTCCCCGCT ACATCGTTAG CCAACTGGGC
    CTCACACGGG ATCCCCTAGA GGAAATGGCC CAGTTGAGCA GCTATGACAG TCCTGATACT
    CCGGAGACAG ATGATTCAGT CGAGGGCCGT GGGGCATCTC TGACATCGAA AAAGACACCC
    TCTGAGGAGG ACTTCGAAAC CATTAAGCTC ATCAGCAATG GCGCCTATGG GGCCGTGTTC
    CTGGTGCGGC ACAAGTCCAC CCGGCAGCGC TTTGCCATGA AGAAGATCAA CAAGCAGAAC
    CTGATCCTGC GGAACCAGAT CCAGCAGGCC TTCGTGGAGC GGGACATCCT GACTTTTGCT
    GAGAACCCCT TTGTGGTCAG CATGTTCTGC TCCTTTGAAA CCAAGCGCCA CCTGTGCATG
    GTGATGGAGT ATGTGGAAGG AGGAGACTGT GCCACGCTGC TGAAGAACAT CGGGGCCCTG
    CCTGTGGACA TGGTGCGCCT GTACTTTGCA GAAACTGTGC TAGCCCTGGA GTACTTACAC
    AATTACGGCA TCGTGCACCG TGACCTCAAG CCTGACAACC TGCTGATTAC ATCCATGGGT
    CACATCAAGC TCACTGATTT TGGCCTTTCC AAAATTGGCC TTATGAGTCT GACCACAAAC
    TTGTACGAGG GCCACATTGA AAAGGATGCA CGGGAGTTCC TGGACAAGCA GGTATGCGGG
    ACCCCGGAGT ACATTGCACC TGAGGTGATC CTGCGGCAGG GCTACGGGAA GCCAGTGGAC
    TGGTGGGCTA TGGGCATCAT CCTGTACGAG TTCCTGGTGG GCTGCGTCCC TTTCTTTGGA
    GACACTCCGG AGGAGCTCTT TGGGCAAGTG ATCAGTGATG AGATTGTGTG GCCTGAGGGG
    GACGATGCAC TGCCGCCGGA CGCCCAAGAT CTCACCTCAA AACTGCTCCA CCAGAACCCA
    TTGGAGAGAC TGGGCACAGG CAGTGCCTAT GAGGTGAAGC AGCACCCATT CTTCACTGGT
    CTGGACTGGA CGGGACTTCT TCGCCAGAAG GCTGAATTTA TTCCTCAGCT GGAGTCAGAG
    GATGATACCA GCTACTTTGA CACCCGCTCA GAGCGATACC ACCACGTGGA CTCGGAGGAT
    GAGGAGGAAG TGAGTGAGGA TGGCTGCCTT GAGATTCGCC AGTTCTCGTC CTGTTCTCCA
    AGGTTCAGCA AGGTGTACAG CAGCATGGAG CGGCTCTCGC TGCTCGAGGA GCGCCGGACG
    CCACCCCCCA CCAAGCGCAG CCTGAGTGAG GAAAAGGAGG ACCGCACGGA TGGCCTGGCG
    GGGCTGAGGG GCCGAGAGCG AAGCTGGGTG ATCGGCTCCC CTGAGATATT ACGGAAGCGG
    CTGTCGGTGT CCGAGTCGTC CCACACGGAA AGCGATTCCA GCCCTCCACT CACCGTGCGG
    CGCCACTGCT CGGGCCTCCT GGACGTGCCT CGCTTCCCTG AGAGCACTGA GGAGGCTGTT
    GGCGCTCCCA GGAGGCAGCA GCAGGAGGGT GCATGGCTTC TGACCCCGTC TGGAGAGGGG
    GCGTCTGGGC CTGCCCTGGA ACGGCCGGCC GAGCGGCGGC TGAAGCTGAA TGAGGAACCT
    GCTGGCCAGA GCAGCGCCCC CAGCCCAGCC ATGGAGACCC GAGGAGGCCG CGGAACCCCA
    CAGCTGGCAG AAGGAGCCAC AGCCAGGGCC ATCAGCGACC TGGCGGTGCG CAGGGCCCGC
    CACCGGCTGC TCTCTGGGGA CTCCATAGAA AAGCGCACCG CTCGCCCCAT CAACAAAGTG
    ATCAAGTCCG CCTCAGCCAC AGCCCTGTCC CTTCTCATTC CTGCTGAGCA CCACTCCTGC
    TCTCCACTGG CCAGCCCCAT GTCCCCACAT TCCCAGTCAT CCAACCCATC ATCCAGGGAC
    TCTTCTCCAA GCAGGGACTT CCTGCCAGCC CTCAGCAGCT CACGGCCCCC CATTGTCATC
    CACCGAGCCG GCAAGAAGTA TGGCTTCACC CTACGAGCCA TCCGTGTCTA CATGGGTGAC
    TCAGATGTCT ACACCGTGCA CCACATGGTG TGGCACGTGG AGGACGGAGG TCCAGCCAGT
    GAGGCAGGGC TGCGTCAGGG GGACCTCATC ACGCACGTCA ACGGGGAGCC TGTGCACGGG
    CTGGTGCACA CGGAGGTGGT GGAGCTGATC CTGAAGAGTG GAAACAAGGT GTCTATTTCA
    ACAACTCCCC TGGAGAACAC CTCCATTAAG GTGGGGCCCG CTCGGAAGGG CAGCTACAAG
    GCCAAGATGG CCCGAAGGAG CAAGCGGAGC CGGGGCAAGG ATGGACAAGA AAGCAGAAAA
    AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC
    CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC
    AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG
    CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT
    CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA
    CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC
    CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC
    CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC
    TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT
    GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG
    AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT
    CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG
    GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG
    CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT
    GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA
    GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG
    CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG
    GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT
    TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA
    GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA
    GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT
    GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA
    ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT
    CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC
    CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG
    TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA
    GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC
    AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG
    TTACCTGATG AGCTTTTAAA GCAAACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024845920.1
    CDS16..5265
    Translation

    Target ORF information:

    RefSeq Version XM_024990152.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990152.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGGATTC TCAAGGATGT GGTAACTGGA CGTAGTCCGC TGCTCTTCAG GAAGCTCAGT 
    AATCCTGACA TATTTTCATC CACTGGAAAA GCTAAACTTC AACGACAACT TAGTCAGGAT
    GACTGTAAGT TACGGAGAGG AAGCCTGGCC AGTTCTTTGT CAGGCAAGCA GCTGCTCCCC
    TTGTCCGGCA GTGCACACAG CAGCGTGGGA CAGGTGACTT GGCAGTCTTC AGGAGAAGCG
    TCCAACCTGG TGCGGATGAG GAATCAGTCC CTCGGACAGT CTGCGCCTTC TCTTACCGCT
    GGCTTGAAGG AGCTGAGCCT TCCAAGAAGA GGCAGCTTCT GTCGGACAAG TAACCGCAAG
    AGTTTGATTG TGACCTCTAG TACGTCACCT ACACTGCCAC GGCCACACTC ACCACTCCAT
    GGCCACACAG GTAACAGTCC TTTGGATAGT CCCCGGAATT TCTCTCCAAA TGCACCTGCT
    CACTTTTCCT TTGTTCCTGC CCGTAGCCAT GGCCACAGAG CAGACAGGAC TGATGGACGG
    CGCTGGTCTT TGGCCTCATT GCCTTCTTCA GGCTATGGAA CCAACACCCC TAGTTCTACC
    GTGTCATCGT CGTGCTCCTC TCAGGAGAAG CTGCACCAGT TGCCTTTCCA GCCCACGGCC
    GATGAGCTGC ACTTCTTGAC GAAGCATTTC AGCACAGACA GTGTGCCTGA TGAGGAGGGG
    CGGCAGTCCC CCGCCCTGCG GCCCCGCTCC CGCAGCCTCA GTCCTGGACG GTCACCAGTT
    TCCTTTGACA GTGAAATAAT AATGATGAAT CATGTATACA AGGAAAGATT CCCCAAGGCC
    ACTGCACAAA TGGAAGAGCG TCTGGCCGAG TTCATCTCCT CCAATACTCC AGACAACGTG
    TTGCCCTTGG CTGATGGAAC CTTGAGCTTT ATTCATCATC AGGTGATAGA GATGGCCCGA
    GACTGCCTGG ACAAATCCCG GAGTGGCCTC ATTACCTCAC ACTACTTCTA CGAACTTCAA
    GAGAATTTAG AGAAGCTTCT GCAAGATGCT CATGAGCGCT CAGAGAGTTC AGAAGTGGCT
    TTTGTGATGC AGCTGGTGAA AAAGCTGATG ATTGTCATTG CCCGCCCAGC TCGTCTCCTG
    GAATGCCTGG AGTTTGACCC TGAAGAGTTC TACCACCTGT TAGAAGCAGC TGAGGGCCAC
    GCCAAAGAGG GACAAGGGAT TAAATGTGAC ATTCCCCGCT ACATCGTTAG CCAACTGGGC
    CTCACACGGG ATCCCCTAGA GGAAATGGCC CAGTTGAGCA GCTATGACAG TCCTGATACT
    CCGGAGACAG ATGATTCAGT CGAGGGCCGT GGGGCATCTC TGACATCGAA AAAGACACCC
    TCTGAGGAGG ACTTCGAAAC CATTAAGCTC ATCAGCAATG GCGCCTATGG GGCCGTGTTC
    CTGGTGCGGC ACAAGTCCAC CCGGCAGCGC TTTGCCATGA AGAAGATCAA CAAGCAGAAC
    CTGATCCTGC GGAACCAGAT CCAGCAGGCC TTCGTGGAGC GGGACATCCT GACTTTTGCT
    GAGAACCCCT TTGTGGTCAG CATGTTCTGC TCCTTTGAAA CCAAGCGCCA CCTGTGCATG
    GTGATGGAGT ATGTGGAAGG AGGAGACTGT GCCACGCTGC TGAAGAACAT CGGGGCCCTG
    CCTGTGGACA TGGTGCGCCT GTACTTTGCA GAAACTGTGC TAGCCCTGGA GTACTTACAC
    AATTACGGCA TCGTGCACCG TGACCTCAAG CCTGACAACC TGCTGATTAC ATCCATGGGT
    CACATCAAGC TCACTGATTT TGGCCTTTCC AAAATTGGCC TTATGAGTCT GACCACAAAC
    TTGTACGAGG GCCACATTGA AAAGGATGCA CGGGAGTTCC TGGACAAGCA GGTATGCGGG
    ACCCCGGAGT ACATTGCACC TGAGGTGATC CTGCGGCAGG GCTACGGGAA GCCAGTGGAC
    TGGTGGGCTA TGGGCATCAT CCTGTACGAG TTCCTGGTGG GCTGCGTCCC TTTCTTTGGA
    GACACTCCGG AGGAGCTCTT TGGGCAAGTG ATCAGTGATG AGATTGTGTG GCCTGAGGGG
    GACGATGCAC TGCCGCCGGA CGCCCAAGAT CTCACCTCAA AACTGCTCCA CCAGAACCCA
    TTGGAGAGAC TGGGCACAGG CAGTGCCTAT GAGGTGAAGC AGCACCCATT CTTCACTGGT
    CTGGACTGGA CGGGACTTCT TCGCCAGAAG GCTGAATTTA TTCCTCAGCT GGAGTCAGAG
    GATGATACCA GCTACTTTGA CACCCGCTCA GAGCGATACC ACCACGTGGA CTCGGAGGAT
    GAGGAGGAAG TGAGTGAGGA TGGCTGCCTT GAGATTCGCC AGTTCTCGTC CTGTTCTCCA
    AGGTTCAGCA AGGTGTACAG CAGCATGGAG CGGCTCTCGC TGCTCGAGGA GCGCCGGACG
    CCACCCCCCA CCAAGCGCAG CCTGAGTGAG GAAAAGGAGG ACCGCACGGA TGGCCTGGCG
    GGGCTGAGGG GCCGAGAGCG AAGCTGGGTG ATCGGCTCCC CTGAGATATT ACGGAAGCGG
    CTGTCGGTGT CCGAGTCGTC CCACACGGAA AGCGATTCCA GCCCTCCACT CACCGTGCGG
    CGCCACTGCT CGGGCCTCCT GGACGTGCCT CGCTTCCCTG AGAGCACTGA GGAGGCTGTT
    GGCGCTCCCA GGAGGCAGCA GCAGGAGGGT GCATGGCTTC TGACCCCGTC TGGAGAGGGG
    GCGTCTGGGC CTGCCCTGGA ACGGCCGGCC GAGCGGCGGC TGAAGCTGAA TGAGGAACCT
    GCTGGCCAGA GCAGCGCCCC CAGCCCAGCC ATGGAGACCC GAGGAGGCCG CGGAACCCCA
    CAGCTGGCAG AAGGAGCCAC AGCCAGGGCC ATCAGCGACC TGGCGGTGCG CAGGGCCCGC
    CACCGGCTGC TCTCTGGGGA CTCCATAGAA AAGCGCACCG CTCGCCCCAT CAACAAAGTG
    ATCAAGTCCG CCTCAGCCAC AGCCCTGTCC CTTCTCATTC CTGCTGAGCA CCACTCCTGC
    TCTCCACTGG CCAGCCCCAT GTCCCCACAT TCCCAGTCAT CCAACCCATC ATCCAGGGAC
    TCTTCTCCAA GCAGGGACTT CCTGCCAGCC CTCAGCAGCT CACGGCCCCC CATTGTCATC
    CACCGAGCCG GCAAGAAGTA TGGCTTCACC CTACGAGCCA TCCGTGTCTA CATGGGTGAC
    TCAGATGTCT ACACCGTGCA CCACATGGTG TGGCACGTGG AGGACGGAGG TCCAGCCAGT
    GAGGCAGGGC TGCGTCAGGG GGACCTCATC ACGCACGTCA ACGGGGAGCC TGTGCACGGG
    CTGGTGCACA CGGAGGTGGT GGAGCTGATC CTGAAGAGTG GAAACAAGGT GTCTATTTCA
    ACAACTCCCC TGGAGAACAC CTCCATTAAG GTGGGGCCCG CTCGGAAGGG CAGCTACAAG
    GCCAAGATGG CCCGAAGGAG CAAGCGGAGC CGGGGCAAGG ATGGACAAGA AAGCAGAAAA
    AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC
    CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC
    AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG
    CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT
    CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA
    CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC
    CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC
    CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC
    TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT
    GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG
    AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT
    CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG
    GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG
    CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT
    GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA
    GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG
    CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG
    GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT
    TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA
    GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA
    GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT
    GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA
    ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT
    CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC
    CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG
    TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA
    GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC
    AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG
    TTACCTGATG AGCTTTTAAA GCAAACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18162
    Clone ID Related Accession (Same CDS sequence) XM_005204883.3 , XM_015462788.1
    Accession Version XM_005204883.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204883.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
    CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG
    TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT
    TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG
    TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA
    CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC
    CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGCCATGGC
    CACAGAGCAG ACAGGACTGA TGGACGGCGC TGGTCTTTGG CCTCATTGCC TTCTTCAGGC
    TATGGAACCA ACACCCCTAG TTCTACCGTG TCATCGTCGT GCTCCTCTCA GGAGAAGCTG
    CACCAGTTGC CTTTCCAGCC CACGGCCGAT GAGCTGCACT TCTTGACGAA GCATTTCAGC
    ACAGACAGTG TGCCTGATGA GGAGGGGCGG CAGTCCCCCG CCCTGCGGCC CCGCTCCCGC
    AGCCTCAGTC CTGGACGGTC ACCAGTTTCC TTTGACAGTG AAATAATAAT GATGAATCAT
    GTATACAAGG AAAGATTCCC CAAGGCCACT GCACAAATGG AAGAGCGTCT GGCCGAGTTC
    ATCTCCTCCA ATACTCCAGA CAACGTGTTG CCCTTGGCTG ATGGAACCTT GAGCTTTATT
    CATCATCAGG TGATAGAGAT GGCCCGAGAC TGCCTGGACA AATCCCGGAG TGGCCTCATT
    ACCTCACACT ACTTCTACGA ACTTCAAGAG AATTTAGAGA AGCTTCTGCA AGATGCTCAT
    GAGCGCTCAG AGAGTTCAGA AGTGGCTTTT GTGATGCAGC TGGTGAAAAA GCTGATGATT
    GTCATTGCCC GCCCAGCTCG TCTCCTGGAA TGCCTGGAGT TTGACCCTGA AGAGTTCTAC
    CACCTGTTAG AAGCAGCTGA GGGCCACGCC AAAGAGGGAC AAGGGATTAA ATGTGACATT
    CCCCGCTACA TCGTTAGCCA ACTGGGCCTC ACACGGGATC CCCTAGAGGA AATGGCCCAG
    TTGAGCAGCT ATGACAGTCC TGATACTCCG GAGACAGATG ATTCAGTCGA GGGCCGTGGG
    GCATCTCTGA CATCGAAAAA GACACCCTCT GAGGAGGACT TCGAAACCAT TAAGCTCATC
    AGCAATGGCG CCTATGGGGC CGTGTTCCTG GTGCGGCACA AGTCCACCCG GCAGCGCTTT
    GCCATGAAGA AGATCAACAA GCAGAACCTG ATCCTGCGGA ACCAGATCCA GCAGGCCTTC
    GTGGAGCGGG ACATCCTGAC TTTTGCTGAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC
    TTTGAAACCA AGCGCCACCT GTGCATGGTG ATGGAGTATG TGGAAGGAGG AGACTGTGCC
    ACGCTGCTGA AGAACATCGG GGCCCTGCCT GTGGACATGG TGCGCCTGTA CTTTGCAGAA
    ACTGTGCTAG CCCTGGAGTA CTTACACAAT TACGGCATCG TGCACCGTGA CCTCAAGCCT
    GACAACCTGC TGATTACATC CATGGGTCAC ATCAAGCTCA CTGATTTTGG CCTTTCCAAA
    ATTGGCCTTA TGAGTCTGAC CACAAACTTG TACGAGGGCC ACATTGAAAA GGATGCACGG
    GAGTTCCTGG ACAAGCAGGT ATGCGGGACC CCGGAGTACA TTGCACCTGA GGTGATCCTG
    CGGCAGGGCT ACGGGAAGCC AGTGGACTGG TGGGCTATGG GCATCATCCT GTACGAGTTC
    CTGGTGGGCT GCGTCCCTTT CTTTGGAGAC ACTCCGGAGG AGCTCTTTGG GCAAGTGATC
    AGTGATGAGA TTGTGTGGCC TGAGGGGGAC GATGCACTGC CGCCGGACGC CCAAGATCTC
    ACCTCAAAAC TGCTCCACCA GAACCCATTG GAGAGACTGG GCACAGGCAG TGCCTATGAG
    GTGAAGCAGC ACCCATTCTT CACTGGTCTG GACTGGACGG GACTTCTTCG CCAGAAGGCT
    GAATTTATTC CTCAGCTGGA GTCAGAGGAT GATACCAGCT ACTTTGACAC CCGCTCAGAG
    CGATACCACC ACGTGGACTC GGAGGATGAG GAGGAAGTGA GTGAGGATGG CTGCCTTGAG
    ATTCGCCAGT TCTCGTCCTG TTCTCCAAGG TTCAGCAAGG TGTACAGCAG CATGGAGCGG
    CTCTCGCTGC TCGAGGAGCG CCGGACGCCA CCCCCCACCA AGCGCAGCCT GAGTGAGGAA
    AAGGAGGACC GCACGGATGG CCTGGCGGGG CTGAGGGGCC GAGAGCGAAG CTGGGTGATC
    GGCTCCCCTG AGATATTACG GAAGCGGCTG TCGGTGTCCG AGTCGTCCCA CACGGAAAGC
    GATTCCAGCC CTCCACTCAC CGTGCGGCGC CACTGCTCGG GCCTCCTGGA CGTGCCTCGC
    TTCCCTGAGA GCACTGAGGA GGCTGTTGGC GCTCCCAGGA GGCAGCAGCA GGAGGGTGCA
    TGGCTTCTGA CCCCGTCTGG AGAGGGGGCG TCTGGGCCTG CCCTGGAACG GCCGGCCGAG
    CGGCGGCTGA AGCTGAATGA GGAACCTGCT GGCCAGAGCA GCGCCCCCAG CCCAGCCATG
    GAGACCCGAG GAGGCCGCGG AACCCCACAG CTGGCAGAAG GAGCCACAGC CAGGGCCATC
    AGCGACCTGG CGGTGCGCAG GGCCCGCCAC CGGCTGCTCT CTGGGGACTC CATAGAAAAG
    CGCACCGCTC GCCCCATCAA CAAAGTGATC AAGTCCGCCT CAGCCACAGC CCTGTCCCTT
    CTCATTCCTG CTGAGCACCA CTCCTGCTCT CCACTGGCCA GCCCCATGTC CCCACATTCC
    CAGTCATCCA ACCCATCATC CAGGGACTCT TCTCCAAGCA GGGACTTCCT GCCAGCCCTC
    AGCAGCTCAC GGCCCCCCAT TGTCATCCAC CGAGCCGGCA AGAAGTATGG CTTCACCCTA
    CGAGCCATCC GTGTCTACAT GGGTGACTCA GATGTCTACA CCGTGCACCA CATGGTGTGG
    CACGTGGAGG ACGGAGGTCC AGCCAGTGAG GCAGGGCTGC GTCAGGGGGA CCTCATCACG
    CACGTCAACG GGGAGCCTGT GCACGGGCTG GTGCACACGG AGGTGGTGGA GCTGATCCTG
    AAGAGTGGAA ACAAGGTGTC TATTTCAACA ACTCCCCTGG AGAACACCTC CATTAAGGTG
    GGGCCCGCTC GGAAGGGCAG CTACAAGGCC AAGATGGCCC GAAGGAGCAA GCGGAGCCGG
    GGCAAGGATG GACAAGAAAG CAGAAAAAGG AGCTCCCTGT TCCGGAAGAT CACCAAGCAG
    GCATCCCTGC TCCACACCAG CCGCAGCCTT TCTTCCCTTA ACCGCTCCCT GTCGTCCGGC
    GAGAGTGGGC CAGGCTCGCC CACACACAGC CACAGCCTTT CTCCCCGCTC TCCCACGCAG
    GGCTACCGGG TGACCCCTGA AGCCGTGCAC TCAGTGGGGG GGAATTCATC ACAGAGCAGC
    TCCCCCAGCT CCAGCGTGCC CAGTTCTCCT GCCGGCTCTG GGCACACACG GCCGAGCTCC
    CTCCACGGTC TGGCGCCCAA GCTCCAACGC CAGTACCGCT CTCCACGGCG CAAGTCAGCA
    GGCAGCATCC CGCTGTCACC ACTGGCCCAC ACCCCTTCAC CACCACCAGC GGCTTCGCCT
    CAGCGTTCCC CATCACCTCT GTCTGGCCAC GGGGCCCAGG CCTTCCCTAC CAAGCTCCAC
    TTGTCGCCTC CCCTAGGCCG GCAACTCTCA CGGCCCAAGA GCGCGGAGCC ACCCCGCTCA
    CCACTACTTA AGAGGGTGCA GTCAGCTGAA AAACTGGCAG CTGCACTGGC TGCTTCTGAG
    AAGAAGCTCG CCACTTCTCG AAAACAGAGC CTTGACCTGC CCCACCCCGA GCTAAAGAAG
    GAATTGCCGC CCAGGGAAGT CAGCTCTCTG GAGGTAGTTG GGACCAGGAG TGTGCTGTCT
    GGAAAGGGGG CGCTGCCAGG GAAAGGGGTG TTGCAGGCTG CTCCCTCACG GGCCTTAGGG
    ACCCTCCGCC AGGACAGGGC TGAACGGCGA GAGTCGCTGC AGAAGCAGGA AGCCATCCGG
    GAGGTTGACT CTTCAGAAGA TGATACTGAT GAGGGGCCTG AGAACAGCCA AGGTGTTCAG
    GAGCCAAGCT TGGTACCCCA CCGAGAAGGG GGCCAGGATC CACCCCTCAG AGGAGCAGGA
    GAGGGTGAGG AAGAGGATGC CTTCTTGCCC AGGGATCCCA GGGGACAGGG CCCAGTGGTC
    ACAGGTCTAC TGACAGGGGT CACACTGGGA TCTCCCAGAA TGGAAGGCCC TGGTGTCCCC
    CAGAAGAAGC TTGGAATCCT ACAGGCTTTT GAAGAGGGTT CCAGCTCCTC GTCCTCAGCC
    CCCAACCTGG GGAAGGCTGG CCCCACAGAC CCCATCCCCG CTGAAGGCTG CTGGAAGGCC
    CAGCACCTCC ACACCCAGGC ACTGACAGCA CTTTGTCCGA GCTCATCAGG ACGCATCCCT
    GCCAGTTGCT CCACTGCCTC CACCCCTGGA GAGCTGGGCC CATGGTCCTG GAAATTCCTC
    TTCAAGGGCC CTGACAGGGC ATACCCAACC GGAAAGGCAG CAATGGAAGA TGGGCCAGCC
    AGCTCCCAAG ATTTGGAAAC TACAACTCCA GGCCACCCTG CAAACCTGTC CCCCAGACAG
    GAGGGGAAGT CATGGCCACC CAGTGCCCCT GGGCTGGCCC ATCCACCTTG TGAGGTTCCC
    AGCCAGAGCT GGCTATGGGA GTCTGAGTGT GCACAGAGAG AGAAAGAGGA GCCAGCGCTG
    GGCATCACCA AAGTGCCTGA TGCCTCAGGT GACAGGAGGC AGGACGTTCC ATGCAAGAGC
    TGCCCCTTCA CCCCAGAGCC TGGGCCCAGC CTGCTCCAGA AGGGCCAAGA CCCAGGGGGC
    CCTCAGGAGC ATCAGGACTT GGCATTGTTA CCTGATGAGC TTTTAAAGCA AACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005204940.1
    CDS268..5424
    Translation

    Target ORF information:

    RefSeq Version XM_005204883.3
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204883.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
    CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG
    TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT
    TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG
    TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA
    CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC
    CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGCCATGGC
    CACAGAGCAG ACAGGACTGA TGGACGGCGC TGGTCTTTGG CCTCATTGCC TTCTTCAGGC
    TATGGAACCA ACACCCCTAG TTCTACCGTG TCATCGTCGT GCTCCTCTCA GGAGAAGCTG
    CACCAGTTGC CTTTCCAGCC CACGGCCGAT GAGCTGCACT TCTTGACGAA GCATTTCAGC
    ACAGACAGTG TGCCTGATGA GGAGGGGCGG CAGTCCCCCG CCCTGCGGCC CCGCTCCCGC
    AGCCTCAGTC CTGGACGGTC ACCAGTTTCC TTTGACAGTG AAATAATAAT GATGAATCAT
    GTATACAAGG AAAGATTCCC CAAGGCCACT GCACAAATGG AAGAGCGTCT GGCCGAGTTC
    ATCTCCTCCA ATACTCCAGA CAACGTGTTG CCCTTGGCTG ATGGAACCTT GAGCTTTATT
    CATCATCAGG TGATAGAGAT GGCCCGAGAC TGCCTGGACA AATCCCGGAG TGGCCTCATT
    ACCTCACACT ACTTCTACGA ACTTCAAGAG AATTTAGAGA AGCTTCTGCA AGATGCTCAT
    GAGCGCTCAG AGAGTTCAGA AGTGGCTTTT GTGATGCAGC TGGTGAAAAA GCTGATGATT
    GTCATTGCCC GCCCAGCTCG TCTCCTGGAA TGCCTGGAGT TTGACCCTGA AGAGTTCTAC
    CACCTGTTAG AAGCAGCTGA GGGCCACGCC AAAGAGGGAC AAGGGATTAA ATGTGACATT
    CCCCGCTACA TCGTTAGCCA ACTGGGCCTC ACACGGGATC CCCTAGAGGA AATGGCCCAG
    TTGAGCAGCT ATGACAGTCC TGATACTCCG GAGACAGATG ATTCAGTCGA GGGCCGTGGG
    GCATCTCTGA CATCGAAAAA GACACCCTCT GAGGAGGACT TCGAAACCAT TAAGCTCATC
    AGCAATGGCG CCTATGGGGC CGTGTTCCTG GTGCGGCACA AGTCCACCCG GCAGCGCTTT
    GCCATGAAGA AGATCAACAA GCAGAACCTG ATCCTGCGGA ACCAGATCCA GCAGGCCTTC
    GTGGAGCGGG ACATCCTGAC TTTTGCTGAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC
    TTTGAAACCA AGCGCCACCT GTGCATGGTG ATGGAGTATG TGGAAGGAGG AGACTGTGCC
    ACGCTGCTGA AGAACATCGG GGCCCTGCCT GTGGACATGG TGCGCCTGTA CTTTGCAGAA
    ACTGTGCTAG CCCTGGAGTA CTTACACAAT TACGGCATCG TGCACCGTGA CCTCAAGCCT
    GACAACCTGC TGATTACATC CATGGGTCAC ATCAAGCTCA CTGATTTTGG CCTTTCCAAA
    ATTGGCCTTA TGAGTCTGAC CACAAACTTG TACGAGGGCC ACATTGAAAA GGATGCACGG
    GAGTTCCTGG ACAAGCAGGT ATGCGGGACC CCGGAGTACA TTGCACCTGA GGTGATCCTG
    CGGCAGGGCT ACGGGAAGCC AGTGGACTGG TGGGCTATGG GCATCATCCT GTACGAGTTC
    CTGGTGGGCT GCGTCCCTTT CTTTGGAGAC ACTCCGGAGG AGCTCTTTGG GCAAGTGATC
    AGTGATGAGA TTGTGTGGCC TGAGGGGGAC GATGCACTGC CGCCGGACGC CCAAGATCTC
    ACCTCAAAAC TGCTCCACCA GAACCCATTG GAGAGACTGG GCACAGGCAG TGCCTATGAG
    GTGAAGCAGC ACCCATTCTT CACTGGTCTG GACTGGACGG GACTTCTTCG CCAGAAGGCT
    GAATTTATTC CTCAGCTGGA GTCAGAGGAT GATACCAGCT ACTTTGACAC CCGCTCAGAG
    CGATACCACC ACGTGGACTC GGAGGATGAG GAGGAAGTGA GTGAGGATGG CTGCCTTGAG
    ATTCGCCAGT TCTCGTCCTG TTCTCCAAGG TTCAGCAAGG TGTACAGCAG CATGGAGCGG
    CTCTCGCTGC TCGAGGAGCG CCGGACGCCA CCCCCCACCA AGCGCAGCCT GAGTGAGGAA
    AAGGAGGACC GCACGGATGG CCTGGCGGGG CTGAGGGGCC GAGAGCGAAG CTGGGTGATC
    GGCTCCCCTG AGATATTACG GAAGCGGCTG TCGGTGTCCG AGTCGTCCCA CACGGAAAGC
    GATTCCAGCC CTCCACTCAC CGTGCGGCGC CACTGCTCGG GCCTCCTGGA CGTGCCTCGC
    TTCCCTGAGA GCACTGAGGA GGCTGTTGGC GCTCCCAGGA GGCAGCAGCA GGAGGGTGCA
    TGGCTTCTGA CCCCGTCTGG AGAGGGGGCG TCTGGGCCTG CCCTGGAACG GCCGGCCGAG
    CGGCGGCTGA AGCTGAATGA GGAACCTGCT GGCCAGAGCA GCGCCCCCAG CCCAGCCATG
    GAGACCCGAG GAGGCCGCGG AACCCCACAG CTGGCAGAAG GAGCCACAGC CAGGGCCATC
    AGCGACCTGG CGGTGCGCAG GGCCCGCCAC CGGCTGCTCT CTGGGGACTC CATAGAAAAG
    CGCACCGCTC GCCCCATCAA CAAAGTGATC AAGTCCGCCT CAGCCACAGC CCTGTCCCTT
    CTCATTCCTG CTGAGCACCA CTCCTGCTCT CCACTGGCCA GCCCCATGTC CCCACATTCC
    CAGTCATCCA ACCCATCATC CAGGGACTCT TCTCCAAGCA GGGACTTCCT GCCAGCCCTC
    AGCAGCTCAC GGCCCCCCAT TGTCATCCAC CGAGCCGGCA AGAAGTATGG CTTCACCCTA
    CGAGCCATCC GTGTCTACAT GGGTGACTCA GATGTCTACA CCGTGCACCA CATGGTGTGG
    CACGTGGAGG ACGGAGGTCC AGCCAGTGAG GCAGGGCTGC GTCAGGGGGA CCTCATCACG
    CACGTCAACG GGGAGCCTGT GCACGGGCTG GTGCACACGG AGGTGGTGGA GCTGATCCTG
    AAGAGTGGAA ACAAGGTGTC TATTTCAACA ACTCCCCTGG AGAACACCTC CATTAAGGTG
    GGGCCCGCTC GGAAGGGCAG CTACAAGGCC AAGATGGCCC GAAGGAGCAA GCGGAGCCGG
    GGCAAGGATG GACAAGAAAG CAGAAAAAGG AGCTCCCTGT TCCGGAAGAT CACCAAGCAG
    GCATCCCTGC TCCACACCAG CCGCAGCCTT TCTTCCCTTA ACCGCTCCCT GTCGTCCGGC
    GAGAGTGGGC CAGGCTCGCC CACACACAGC CACAGCCTTT CTCCCCGCTC TCCCACGCAG
    GGCTACCGGG TGACCCCTGA AGCCGTGCAC TCAGTGGGGG GGAATTCATC ACAGAGCAGC
    TCCCCCAGCT CCAGCGTGCC CAGTTCTCCT GCCGGCTCTG GGCACACACG GCCGAGCTCC
    CTCCACGGTC TGGCGCCCAA GCTCCAACGC CAGTACCGCT CTCCACGGCG CAAGTCAGCA
    GGCAGCATCC CGCTGTCACC ACTGGCCCAC ACCCCTTCAC CACCACCAGC GGCTTCGCCT
    CAGCGTTCCC CATCACCTCT GTCTGGCCAC GGGGCCCAGG CCTTCCCTAC CAAGCTCCAC
    TTGTCGCCTC CCCTAGGCCG GCAACTCTCA CGGCCCAAGA GCGCGGAGCC ACCCCGCTCA
    CCACTACTTA AGAGGGTGCA GTCAGCTGAA AAACTGGCAG CTGCACTGGC TGCTTCTGAG
    AAGAAGCTCG CCACTTCTCG AAAACAGAGC CTTGACCTGC CCCACCCCGA GCTAAAGAAG
    GAATTGCCGC CCAGGGAAGT CAGCTCTCTG GAGGTAGTTG GGACCAGGAG TGTGCTGTCT
    GGAAAGGGGG CGCTGCCAGG GAAAGGGGTG TTGCAGGCTG CTCCCTCACG GGCCTTAGGG
    ACCCTCCGCC AGGACAGGGC TGAACGGCGA GAGTCGCTGC AGAAGCAGGA AGCCATCCGG
    GAGGTTGACT CTTCAGAAGA TGATACTGAT GAGGGGCCTG AGAACAGCCA AGGTGTTCAG
    GAGCCAAGCT TGGTACCCCA CCGAGAAGGG GGCCAGGATC CACCCCTCAG AGGAGCAGGA
    GAGGGTGAGG AAGAGGATGC CTTCTTGCCC AGGGATCCCA GGGGACAGGG CCCAGTGGTC
    ACAGGTCTAC TGACAGGGGT CACACTGGGA TCTCCCAGAA TGGAAGGCCC TGGTGTCCCC
    CAGAAGAAGC TTGGAATCCT ACAGGCTTTT GAAGAGGGTT CCAGCTCCTC GTCCTCAGCC
    CCCAACCTGG GGAAGGCTGG CCCCACAGAC CCCATCCCCG CTGAAGGCTG CTGGAAGGCC
    CAGCACCTCC ACACCCAGGC ACTGACAGCA CTTTGTCCGA GCTCATCAGG ACGCATCCCT
    GCCAGTTGCT CCACTGCCTC CACCCCTGGA GAGCTGGGCC CATGGTCCTG GAAATTCCTC
    TTCAAGGGCC CTGACAGGGC ATACCCAACC GGAAAGGCAG CAATGGAAGA TGGGCCAGCC
    AGCTCCCAAG ATTTGGAAAC TACAACTCCA GGCCACCCTG CAAACCTGTC CCCCAGACAG
    GAGGGGAAGT CATGGCCACC CAGTGCCCCT GGGCTGGCCC ATCCACCTTG TGAGGTTCCC
    AGCCAGAGCT GGCTATGGGA GTCTGAGTGT GCACAGAGAG AGAAAGAGGA GCCAGCGCTG
    GGCATCACCA AAGTGCCTGA TGCCTCAGGT GACAGGAGGC AGGACGTTCC ATGCAAGAGC
    TGCCCCTTCA CCCCAGAGCC TGGGCCCAGC CTGCTCCAGA AGGGCCAAGA CCCAGGGGGC
    CCTCAGGAGC ATCAGGACTT GGCATTGTTA CCTGATGAGC TTTTAAAGCA AACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18162
    Clone ID Related Accession (Same CDS sequence) XM_005204883.3 , XM_015462788.1
    Accession Version XM_015462788.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
    CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG
    TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT
    TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG
    TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA
    CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC
    CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGCCATGGC
    CACAGAGCAG ACAGGACTGA TGGACGGCGC TGGTCTTTGG CCTCATTGCC TTCTTCAGGC
    TATGGAACCA ACACCCCTAG TTCTACCGTG TCATCGTCGT GCTCCTCTCA GGAGAAGCTG
    CACCAGTTGC CTTTCCAGCC CACGGCCGAT GAGCTGCACT TCTTGACGAA GCATTTCAGC
    ACAGACAGTG TGCCTGATGA GGAGGGGCGG CAGTCCCCCG CCCTGCGGCC CCGCTCCCGC
    AGCCTCAGTC CTGGACGGTC ACCAGTTTCC TTTGACAGTG AAATAATAAT GATGAATCAT
    GTATACAAGG AAAGATTCCC CAAGGCCACT GCACAAATGG AAGAGCGTCT GGCCGAGTTC
    ATCTCCTCCA ATACTCCAGA CAACGTGTTG CCCTTGGCTG ATGGAACCTT GAGCTTTATT
    CATCATCAGG TGATAGAGAT GGCCCGAGAC TGCCTGGACA AATCCCGGAG TGGCCTCATT
    ACCTCACACT ACTTCTACGA ACTTCAAGAG AATTTAGAGA AGCTTCTGCA AGATGCTCAT
    GAGCGCTCAG AGAGTTCAGA AGTGGCTTTT GTGATGCAGC TGGTGAAAAA GCTGATGATT
    GTCATTGCCC GCCCAGCTCG TCTCCTGGAA TGCCTGGAGT TTGACCCTGA AGAGTTCTAC
    CACCTGTTAG AAGCAGCTGA GGGCCACGCC AAAGAGGGAC AAGGGATTAA ATGTGACATT
    CCCCGCTACA TCGTTAGCCA ACTGGGCCTC ACACGGGATC CCCTAGAGGA AATGGCCCAG
    TTGAGCAGCT ATGACAGTCC TGATACTCCG GAGACAGATG ATTCAGTCGA GGGCCGTGGG
    GCATCTCTGA CATCGAAAAA GACACCCTCT GAGGAGGACT TCGAAACCAT TAAGCTCATC
    AGCAATGGCG CCTATGGGGC CGTGTTCCTG GTGCGGCACA AGTCCACCCG GCAGCGCTTT
    GCCATGAAGA AGATCAACAA GCAGAACCTG ATCCTGCGGA ACCAGATCCA GCAGGCCTTC
    GTGGAGCGGG ACATCCTGAC TTTTGCTGAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC
    TTTGAAACCA AGCGCCACCT GTGCATGGTG ATGGAGTATG TGGAAGGAGG AGACTGTGCC
    ACGCTGCTGA AGAACATCGG GGCCCTGCCT GTGGACATGG TGCGCCTGTA CTTTGCAGAA
    ACTGTGCTAG CCCTGGAGTA CTTACACAAT TACGGCATCG TGCACCGTGA CCTCAAGCCT
    GACAACCTGC TGATTACATC CATGGGTCAC ATCAAGCTCA CTGATTTTGG CCTTTCCAAA
    ATTGGCCTTA TGAGTCTGAC CACAAACTTG TACGAGGGCC ACATTGAAAA GGATGCACGG
    GAGTTCCTGG ACAAGCAGGT ATGCGGGACC CCGGAGTACA TTGCACCTGA GGTGATCCTG
    CGGCAGGGCT ACGGGAAGCC AGTGGACTGG TGGGCTATGG GCATCATCCT GTACGAGTTC
    CTGGTGGGCT GCGTCCCTTT CTTTGGAGAC ACTCCGGAGG AGCTCTTTGG GCAAGTGATC
    AGTGATGAGA TTGTGTGGCC TGAGGGGGAC GATGCACTGC CGCCGGACGC CCAAGATCTC
    ACCTCAAAAC TGCTCCACCA GAACCCATTG GAGAGACTGG GCACAGGCAG TGCCTATGAG
    GTGAAGCAGC ACCCATTCTT CACTGGTCTG GACTGGACGG GACTTCTTCG CCAGAAGGCT
    GAATTTATTC CTCAGCTGGA GTCAGAGGAT GATACCAGCT ACTTTGACAC CCGCTCAGAG
    CGATACCACC ACGTGGACTC GGAGGATGAG GAGGAAGTGA GTGAGGATGG CTGCCTTGAG
    ATTCGCCAGT TCTCGTCCTG TTCTCCAAGG TTCAGCAAGG TGTACAGCAG CATGGAGCGG
    CTCTCGCTGC TCGAGGAGCG CCGGACGCCA CCCCCCACCA AGCGCAGCCT GAGTGAGGAA
    AAGGAGGACC GCACGGATGG CCTGGCGGGG CTGAGGGGCC GAGAGCGAAG CTGGGTGATC
    GGCTCCCCTG AGATATTACG GAAGCGGCTG TCGGTGTCCG AGTCGTCCCA CACGGAAAGC
    GATTCCAGCC CTCCACTCAC CGTGCGGCGC CACTGCTCGG GCCTCCTGGA CGTGCCTCGC
    TTCCCTGAGA GCACTGAGGA GGCTGTTGGC GCTCCCAGGA GGCAGCAGCA GGAGGGTGCA
    TGGCTTCTGA CCCCGTCTGG AGAGGGGGCG TCTGGGCCTG CCCTGGAACG GCCGGCCGAG
    CGGCGGCTGA AGCTGAATGA GGAACCTGCT GGCCAGAGCA GCGCCCCCAG CCCAGCCATG
    GAGACCCGAG GAGGCCGCGG AACCCCACAG CTGGCAGAAG GAGCCACAGC CAGGGCCATC
    AGCGACCTGG CGGTGCGCAG GGCCCGCCAC CGGCTGCTCT CTGGGGACTC CATAGAAAAG
    CGCACCGCTC GCCCCATCAA CAAAGTGATC AAGTCCGCCT CAGCCACAGC CCTGTCCCTT
    CTCATTCCTG CTGAGCACCA CTCCTGCTCT CCACTGGCCA GCCCCATGTC CCCACATTCC
    CAGTCATCCA ACCCATCATC CAGGGACTCT TCTCCAAGCA GGGACTTCCT GCCAGCCCTC
    AGCAGCTCAC GGCCCCCCAT TGTCATCCAC CGAGCCGGCA AGAAGTATGG CTTCACCCTA
    CGAGCCATCC GTGTCTACAT GGGTGACTCA GATGTCTACA CCGTGCACCA CATGGTGTGG
    CACGTGGAGG ACGGAGGTCC AGCCAGTGAG GCAGGGCTGC GTCAGGGGGA CCTCATCACG
    CACGTCAACG GGGAGCCTGT GCACGGGCTG GTGCACACGG AGGTGGTGGA GCTGATCCTG
    AAGAGTGGAA ACAAGGTGTC TATTTCAACA ACTCCCCTGG AGAACACCTC CATTAAGGTG
    GGGCCCGCTC GGAAGGGCAG CTACAAGGCC AAGATGGCCC GAAGGAGCAA GCGGAGCCGG
    GGCAAGGATG GACAAGAAAG CAGAAAAAGG AGCTCCCTGT TCCGGAAGAT CACCAAGCAG
    GCATCCCTGC TCCACACCAG CCGCAGCCTT TCTTCCCTTA ACCGCTCCCT GTCGTCCGGC
    GAGAGTGGGC CAGGCTCGCC CACACACAGC CACAGCCTTT CTCCCCGCTC TCCCACGCAG
    GGCTACCGGG TGACCCCTGA AGCCGTGCAC TCAGTGGGGG GGAATTCATC ACAGAGCAGC
    TCCCCCAGCT CCAGCGTGCC CAGTTCTCCT GCCGGCTCTG GGCACACACG GCCGAGCTCC
    CTCCACGGTC TGGCGCCCAA GCTCCAACGC CAGTACCGCT CTCCACGGCG CAAGTCAGCA
    GGCAGCATCC CGCTGTCACC ACTGGCCCAC ACCCCTTCAC CACCACCAGC GGCTTCGCCT
    CAGCGTTCCC CATCACCTCT GTCTGGCCAC GGGGCCCAGG CCTTCCCTAC CAAGCTCCAC
    TTGTCGCCTC CCCTAGGCCG GCAACTCTCA CGGCCCAAGA GCGCGGAGCC ACCCCGCTCA
    CCACTACTTA AGAGGGTGCA GTCAGCTGAA AAACTGGCAG CTGCACTGGC TGCTTCTGAG
    AAGAAGCTCG CCACTTCTCG AAAACAGAGC CTTGACCTGC CCCACCCCGA GCTAAAGAAG
    GAATTGCCGC CCAGGGAAGT CAGCTCTCTG GAGGTAGTTG GGACCAGGAG TGTGCTGTCT
    GGAAAGGGGG CGCTGCCAGG GAAAGGGGTG TTGCAGGCTG CTCCCTCACG GGCCTTAGGG
    ACCCTCCGCC AGGACAGGGC TGAACGGCGA GAGTCGCTGC AGAAGCAGGA AGCCATCCGG
    GAGGTTGACT CTTCAGAAGA TGATACTGAT GAGGGGCCTG AGAACAGCCA AGGTGTTCAG
    GAGCCAAGCT TGGTACCCCA CCGAGAAGGG GGCCAGGATC CACCCCTCAG AGGAGCAGGA
    GAGGGTGAGG AAGAGGATGC CTTCTTGCCC AGGGATCCCA GGGGACAGGG CCCAGTGGTC
    ACAGGTCTAC TGACAGGGGT CACACTGGGA TCTCCCAGAA TGGAAGGCCC TGGTGTCCCC
    CAGAAGAAGC TTGGAATCCT ACAGGCTTTT GAAGAGGGTT CCAGCTCCTC GTCCTCAGCC
    CCCAACCTGG GGAAGGCTGG CCCCACAGAC CCCATCCCCG CTGAAGGCTG CTGGAAGGCC
    CAGCACCTCC ACACCCAGGC ACTGACAGCA CTTTGTCCGA GCTCATCAGG ACGCATCCCT
    GCCAGTTGCT CCACTGCCTC CACCCCTGGA GAGCTGGGCC CATGGTCCTG GAAATTCCTC
    TTCAAGGGCC CTGACAGGGC ATACCCAACC GGAAAGGCAG CAATGGAAGA TGGGCCAGCC
    AGCTCCCAAG ATTTGGAAAC TACAACTCCA GGCCACCCTG CAAACCTGTC CCCCAGACAG
    GAGGGGAAGT CATGGCCACC CAGTGCCCCT GGGCTGGCCC ATCCACCTTG TGAGGTTCCC
    AGCCAGAGCT GGCTATGGGA GTCTGAGTGT GCACAGAGAG AGAAAGAGGA GCCAGCGCTG
    GGCATCACCA AAGTGCCTGA TGCCTCAGGT GACAGGAGGC AGGACGTTCC ATGCAAGAGC
    TGCCCCTTCA CCCCAGAGCC TGGGCCCAGC CTGCTCCAGA AGGGCCAAGA CCCAGGGGGC
    CCTCAGGAGC ATCAGGACTT GGCATTGTTA CCTGATGAGC TTTTAAAGCA AACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318274.1
    CDS268..5424
    Translation

    Target ORF information:

    RefSeq Version XM_015462788.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462788.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
    CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG
    TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT
    TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG
    TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA
    CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC
    CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGCCATGGC
    CACAGAGCAG ACAGGACTGA TGGACGGCGC TGGTCTTTGG CCTCATTGCC TTCTTCAGGC
    TATGGAACCA ACACCCCTAG TTCTACCGTG TCATCGTCGT GCTCCTCTCA GGAGAAGCTG
    CACCAGTTGC CTTTCCAGCC CACGGCCGAT GAGCTGCACT TCTTGACGAA GCATTTCAGC
    ACAGACAGTG TGCCTGATGA GGAGGGGCGG CAGTCCCCCG CCCTGCGGCC CCGCTCCCGC
    AGCCTCAGTC CTGGACGGTC ACCAGTTTCC TTTGACAGTG AAATAATAAT GATGAATCAT
    GTATACAAGG AAAGATTCCC CAAGGCCACT GCACAAATGG AAGAGCGTCT GGCCGAGTTC
    ATCTCCTCCA ATACTCCAGA CAACGTGTTG CCCTTGGCTG ATGGAACCTT GAGCTTTATT
    CATCATCAGG TGATAGAGAT GGCCCGAGAC TGCCTGGACA AATCCCGGAG TGGCCTCATT
    ACCTCACACT ACTTCTACGA ACTTCAAGAG AATTTAGAGA AGCTTCTGCA AGATGCTCAT
    GAGCGCTCAG AGAGTTCAGA AGTGGCTTTT GTGATGCAGC TGGTGAAAAA GCTGATGATT
    GTCATTGCCC GCCCAGCTCG TCTCCTGGAA TGCCTGGAGT TTGACCCTGA AGAGTTCTAC
    CACCTGTTAG AAGCAGCTGA GGGCCACGCC AAAGAGGGAC AAGGGATTAA ATGTGACATT
    CCCCGCTACA TCGTTAGCCA ACTGGGCCTC ACACGGGATC CCCTAGAGGA AATGGCCCAG
    TTGAGCAGCT ATGACAGTCC TGATACTCCG GAGACAGATG ATTCAGTCGA GGGCCGTGGG
    GCATCTCTGA CATCGAAAAA GACACCCTCT GAGGAGGACT TCGAAACCAT TAAGCTCATC
    AGCAATGGCG CCTATGGGGC CGTGTTCCTG GTGCGGCACA AGTCCACCCG GCAGCGCTTT
    GCCATGAAGA AGATCAACAA GCAGAACCTG ATCCTGCGGA ACCAGATCCA GCAGGCCTTC
    GTGGAGCGGG ACATCCTGAC TTTTGCTGAG AACCCCTTTG TGGTCAGCAT GTTCTGCTCC
    TTTGAAACCA AGCGCCACCT GTGCATGGTG ATGGAGTATG TGGAAGGAGG AGACTGTGCC
    ACGCTGCTGA AGAACATCGG GGCCCTGCCT GTGGACATGG TGCGCCTGTA CTTTGCAGAA
    ACTGTGCTAG CCCTGGAGTA CTTACACAAT TACGGCATCG TGCACCGTGA CCTCAAGCCT
    GACAACCTGC TGATTACATC CATGGGTCAC ATCAAGCTCA CTGATTTTGG CCTTTCCAAA
    ATTGGCCTTA TGAGTCTGAC CACAAACTTG TACGAGGGCC ACATTGAAAA GGATGCACGG
    GAGTTCCTGG ACAAGCAGGT ATGCGGGACC CCGGAGTACA TTGCACCTGA GGTGATCCTG
    CGGCAGGGCT ACGGGAAGCC AGTGGACTGG TGGGCTATGG GCATCATCCT GTACGAGTTC
    CTGGTGGGCT GCGTCCCTTT CTTTGGAGAC ACTCCGGAGG AGCTCTTTGG GCAAGTGATC
    AGTGATGAGA TTGTGTGGCC TGAGGGGGAC GATGCACTGC CGCCGGACGC CCAAGATCTC
    ACCTCAAAAC TGCTCCACCA GAACCCATTG GAGAGACTGG GCACAGGCAG TGCCTATGAG
    GTGAAGCAGC ACCCATTCTT CACTGGTCTG GACTGGACGG GACTTCTTCG CCAGAAGGCT
    GAATTTATTC CTCAGCTGGA GTCAGAGGAT GATACCAGCT ACTTTGACAC CCGCTCAGAG
    CGATACCACC ACGTGGACTC GGAGGATGAG GAGGAAGTGA GTGAGGATGG CTGCCTTGAG
    ATTCGCCAGT TCTCGTCCTG TTCTCCAAGG TTCAGCAAGG TGTACAGCAG CATGGAGCGG
    CTCTCGCTGC TCGAGGAGCG CCGGACGCCA CCCCCCACCA AGCGCAGCCT GAGTGAGGAA
    AAGGAGGACC GCACGGATGG CCTGGCGGGG CTGAGGGGCC GAGAGCGAAG CTGGGTGATC
    GGCTCCCCTG AGATATTACG GAAGCGGCTG TCGGTGTCCG AGTCGTCCCA CACGGAAAGC
    GATTCCAGCC CTCCACTCAC CGTGCGGCGC CACTGCTCGG GCCTCCTGGA CGTGCCTCGC
    TTCCCTGAGA GCACTGAGGA GGCTGTTGGC GCTCCCAGGA GGCAGCAGCA GGAGGGTGCA
    TGGCTTCTGA CCCCGTCTGG AGAGGGGGCG TCTGGGCCTG CCCTGGAACG GCCGGCCGAG
    CGGCGGCTGA AGCTGAATGA GGAACCTGCT GGCCAGAGCA GCGCCCCCAG CCCAGCCATG
    GAGACCCGAG GAGGCCGCGG AACCCCACAG CTGGCAGAAG GAGCCACAGC CAGGGCCATC
    AGCGACCTGG CGGTGCGCAG GGCCCGCCAC CGGCTGCTCT CTGGGGACTC CATAGAAAAG
    CGCACCGCTC GCCCCATCAA CAAAGTGATC AAGTCCGCCT CAGCCACAGC CCTGTCCCTT
    CTCATTCCTG CTGAGCACCA CTCCTGCTCT CCACTGGCCA GCCCCATGTC CCCACATTCC
    CAGTCATCCA ACCCATCATC CAGGGACTCT TCTCCAAGCA GGGACTTCCT GCCAGCCCTC
    AGCAGCTCAC GGCCCCCCAT TGTCATCCAC CGAGCCGGCA AGAAGTATGG CTTCACCCTA
    CGAGCCATCC GTGTCTACAT GGGTGACTCA GATGTCTACA CCGTGCACCA CATGGTGTGG
    CACGTGGAGG ACGGAGGTCC AGCCAGTGAG GCAGGGCTGC GTCAGGGGGA CCTCATCACG
    CACGTCAACG GGGAGCCTGT GCACGGGCTG GTGCACACGG AGGTGGTGGA GCTGATCCTG
    AAGAGTGGAA ACAAGGTGTC TATTTCAACA ACTCCCCTGG AGAACACCTC CATTAAGGTG
    GGGCCCGCTC GGAAGGGCAG CTACAAGGCC AAGATGGCCC GAAGGAGCAA GCGGAGCCGG
    GGCAAGGATG GACAAGAAAG CAGAAAAAGG AGCTCCCTGT TCCGGAAGAT CACCAAGCAG
    GCATCCCTGC TCCACACCAG CCGCAGCCTT TCTTCCCTTA ACCGCTCCCT GTCGTCCGGC
    GAGAGTGGGC CAGGCTCGCC CACACACAGC CACAGCCTTT CTCCCCGCTC TCCCACGCAG
    GGCTACCGGG TGACCCCTGA AGCCGTGCAC TCAGTGGGGG GGAATTCATC ACAGAGCAGC
    TCCCCCAGCT CCAGCGTGCC CAGTTCTCCT GCCGGCTCTG GGCACACACG GCCGAGCTCC
    CTCCACGGTC TGGCGCCCAA GCTCCAACGC CAGTACCGCT CTCCACGGCG CAAGTCAGCA
    GGCAGCATCC CGCTGTCACC ACTGGCCCAC ACCCCTTCAC CACCACCAGC GGCTTCGCCT
    CAGCGTTCCC CATCACCTCT GTCTGGCCAC GGGGCCCAGG CCTTCCCTAC CAAGCTCCAC
    TTGTCGCCTC CCCTAGGCCG GCAACTCTCA CGGCCCAAGA GCGCGGAGCC ACCCCGCTCA
    CCACTACTTA AGAGGGTGCA GTCAGCTGAA AAACTGGCAG CTGCACTGGC TGCTTCTGAG
    AAGAAGCTCG CCACTTCTCG AAAACAGAGC CTTGACCTGC CCCACCCCGA GCTAAAGAAG
    GAATTGCCGC CCAGGGAAGT CAGCTCTCTG GAGGTAGTTG GGACCAGGAG TGTGCTGTCT
    GGAAAGGGGG CGCTGCCAGG GAAAGGGGTG TTGCAGGCTG CTCCCTCACG GGCCTTAGGG
    ACCCTCCGCC AGGACAGGGC TGAACGGCGA GAGTCGCTGC AGAAGCAGGA AGCCATCCGG
    GAGGTTGACT CTTCAGAAGA TGATACTGAT GAGGGGCCTG AGAACAGCCA AGGTGTTCAG
    GAGCCAAGCT TGGTACCCCA CCGAGAAGGG GGCCAGGATC CACCCCTCAG AGGAGCAGGA
    GAGGGTGAGG AAGAGGATGC CTTCTTGCCC AGGGATCCCA GGGGACAGGG CCCAGTGGTC
    ACAGGTCTAC TGACAGGGGT CACACTGGGA TCTCCCAGAA TGGAAGGCCC TGGTGTCCCC
    CAGAAGAAGC TTGGAATCCT ACAGGCTTTT GAAGAGGGTT CCAGCTCCTC GTCCTCAGCC
    CCCAACCTGG GGAAGGCTGG CCCCACAGAC CCCATCCCCG CTGAAGGCTG CTGGAAGGCC
    CAGCACCTCC ACACCCAGGC ACTGACAGCA CTTTGTCCGA GCTCATCAGG ACGCATCCCT
    GCCAGTTGCT CCACTGCCTC CACCCCTGGA GAGCTGGGCC CATGGTCCTG GAAATTCCTC
    TTCAAGGGCC CTGACAGGGC ATACCCAACC GGAAAGGCAG CAATGGAAGA TGGGCCAGCC
    AGCTCCCAAG ATTTGGAAAC TACAACTCCA GGCCACCCTG CAAACCTGTC CCCCAGACAG
    GAGGGGAAGT CATGGCCACC CAGTGCCCCT GGGCTGGCCC ATCCACCTTG TGAGGTTCCC
    AGCCAGAGCT GGCTATGGGA GTCTGAGTGT GCACAGAGAG AGAAAGAGGA GCCAGCGCTG
    GGCATCACCA AAGTGCCTGA TGCCTCAGGT GACAGGAGGC AGGACGTTCC ATGCAAGAGC
    TGCCCCTTCA CCCCAGAGCC TGGGCCCAGC CTGCTCCAGA AGGGCCAAGA CCCAGGGGGC
    CCTCAGGAGC ATCAGGACTT GGCATTGTTA CCTGATGAGC TTTTAAAGCA AACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18163
    Clone ID Related Accession (Same CDS sequence) XM_015462789.1 , XM_005204884.3
    Accession Version XM_015462789.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5136bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
    CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG
    TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT
    TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG
    TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA
    CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC
    CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGGACTGAT
    GGACGGCGCT GGTCTTTGGC CTCATTGCCT TCTTCAGGCT ATGGAACCAA CACCCCTAGT
    TCTACCGTGT CATCGTCGTG CTCCTCTCAG GAGAAGCTGC ACCAGTTGCC TTTCCAGCCC
    ACGGCCGATG AGCTGCACTT CTTGACGAAG CATTTCAGCA CAGACAGTGT GCCTGATGAG
    GAGGGGCGGC AGTCCCCCGC CCTGCGGCCC CGCTCCCGCA GCCTCAGTCC TGGACGGTCA
    CCAGTTTCCT TTGACAGTGA AATAATAATG ATGAATCATG TATACAAGGA AAGATTCCCC
    AAGGCCACTG CACAAATGGA AGAGCGTCTG GCCGAGTTCA TCTCCTCCAA TACTCCAGAC
    AACGTGTTGC CCTTGGCTGA TGGAACCTTG AGCTTTATTC ATCATCAGGT GATAGAGATG
    GCCCGAGACT GCCTGGACAA ATCCCGGAGT GGCCTCATTA CCTCACACTA CTTCTACGAA
    CTTCAAGAGA ATTTAGAGAA GCTTCTGCAA GATGCTCATG AGCGCTCAGA GAGTTCAGAA
    GTGGCTTTTG TGATGCAGCT GGTGAAAAAG CTGATGATTG TCATTGCCCG CCCAGCTCGT
    CTCCTGGAAT GCCTGGAGTT TGACCCTGAA GAGTTCTACC ACCTGTTAGA AGCAGCTGAG
    GGCCACGCCA AAGAGGGACA AGGGATTAAA TGTGACATTC CCCGCTACAT CGTTAGCCAA
    CTGGGCCTCA CACGGGATCC CCTAGAGGAA ATGGCCCAGT TGAGCAGCTA TGACAGTCCT
    GATACTCCGG AGACAGATGA TTCAGTCGAG GGCCGTGGGG CATCTCTGAC ATCGAAAAAG
    ACACCCTCTG AGGAGGACTT CGAAACCATT AAGCTCATCA GCAATGGCGC CTATGGGGCC
    GTGTTCCTGG TGCGGCACAA GTCCACCCGG CAGCGCTTTG CCATGAAGAA GATCAACAAG
    CAGAACCTGA TCCTGCGGAA CCAGATCCAG CAGGCCTTCG TGGAGCGGGA CATCCTGACT
    TTTGCTGAGA ACCCCTTTGT GGTCAGCATG TTCTGCTCCT TTGAAACCAA GCGCCACCTG
    TGCATGGTGA TGGAGTATGT GGAAGGAGGA GACTGTGCCA CGCTGCTGAA GAACATCGGG
    GCCCTGCCTG TGGACATGGT GCGCCTGTAC TTTGCAGAAA CTGTGCTAGC CCTGGAGTAC
    TTACACAATT ACGGCATCGT GCACCGTGAC CTCAAGCCTG ACAACCTGCT GATTACATCC
    ATGGGTCACA TCAAGCTCAC TGATTTTGGC CTTTCCAAAA TTGGCCTTAT GAGTCTGACC
    ACAAACTTGT ACGAGGGCCA CATTGAAAAG GATGCACGGG AGTTCCTGGA CAAGCAGGTA
    TGCGGGACCC CGGAGTACAT TGCACCTGAG GTGATCCTGC GGCAGGGCTA CGGGAAGCCA
    GTGGACTGGT GGGCTATGGG CATCATCCTG TACGAGTTCC TGGTGGGCTG CGTCCCTTTC
    TTTGGAGACA CTCCGGAGGA GCTCTTTGGG CAAGTGATCA GTGATGAGAT TGTGTGGCCT
    GAGGGGGACG ATGCACTGCC GCCGGACGCC CAAGATCTCA CCTCAAAACT GCTCCACCAG
    AACCCATTGG AGAGACTGGG CACAGGCAGT GCCTATGAGG TGAAGCAGCA CCCATTCTTC
    ACTGGTCTGG ACTGGACGGG ACTTCTTCGC CAGAAGGCTG AATTTATTCC TCAGCTGGAG
    TCAGAGGATG ATACCAGCTA CTTTGACACC CGCTCAGAGC GATACCACCA CGTGGACTCG
    GAGGATGAGG AGGAAGTGAG TGAGGATGGC TGCCTTGAGA TTCGCCAGTT CTCGTCCTGT
    TCTCCAAGGT TCAGCAAGGT GTACAGCAGC ATGGAGCGGC TCTCGCTGCT CGAGGAGCGC
    CGGACGCCAC CCCCCACCAA GCGCAGCCTG AGTGAGGAAA AGGAGGACCG CACGGATGGC
    CTGGCGGGGC TGAGGGGCCG AGAGCGAAGC TGGGTGATCG GCTCCCCTGA GATATTACGG
    AAGCGGCTGT CGGTGTCCGA GTCGTCCCAC ACGGAAAGCG ATTCCAGCCC TCCACTCACC
    GTGCGGCGCC ACTGCTCGGG CCTCCTGGAC GTGCCTCGCT TCCCTGAGAG CACTGAGGAG
    GCTGTTGGCG CTCCCAGGAG GCAGCAGCAG GAGGGTGCAT GGCTTCTGAC CCCGTCTGGA
    GAGGGGGCGT CTGGGCCTGC CCTGGAACGG CCGGCCGAGC GGCGGCTGAA GCTGAATGAG
    GAACCTGCTG GCCAGAGCAG CGCCCCCAGC CCAGCCATGG AGACCCGAGG AGGCCGCGGA
    ACCCCACAGC TGGCAGAAGG AGCCACAGCC AGGGCCATCA GCGACCTGGC GGTGCGCAGG
    GCCCGCCACC GGCTGCTCTC TGGGGACTCC ATAGAAAAGC GCACCGCTCG CCCCATCAAC
    AAAGTGATCA AGTCCGCCTC AGCCACAGCC CTGTCCCTTC TCATTCCTGC TGAGCACCAC
    TCCTGCTCTC CACTGGCCAG CCCCATGTCC CCACATTCCC AGTCATCCAA CCCATCATCC
    AGGGACTCTT CTCCAAGCAG GGACTTCCTG CCAGCCCTCA GCAGCTCACG GCCCCCCATT
    GTCATCCACC GAGCCGGCAA GAAGTATGGC TTCACCCTAC GAGCCATCCG TGTCTACATG
    GGTGACTCAG ATGTCTACAC CGTGCACCAC ATGGTGTGGC ACGTGGAGGA CGGAGGTCCA
    GCCAGTGAGG CAGGGCTGCG TCAGGGGGAC CTCATCACGC ACGTCAACGG GGAGCCTGTG
    CACGGGCTGG TGCACACGGA GGTGGTGGAG CTGATCCTGA AGAGTGGAAA CAAGGTGTCT
    ATTTCAACAA CTCCCCTGGA GAACACCTCC ATTAAGGTGG GGCCCGCTCG GAAGGGCAGC
    TACAAGGCCA AGATGGCCCG AAGGAGCAAG CGGAGCCGGG GCAAGGATGG ACAAGAAAGC
    AGAAAAAGGA GCTCCCTGTT CCGGAAGATC ACCAAGCAGG CATCCCTGCT CCACACCAGC
    CGCAGCCTTT CTTCCCTTAA CCGCTCCCTG TCGTCCGGCG AGAGTGGGCC AGGCTCGCCC
    ACACACAGCC ACAGCCTTTC TCCCCGCTCT CCCACGCAGG GCTACCGGGT GACCCCTGAA
    GCCGTGCACT CAGTGGGGGG GAATTCATCA CAGAGCAGCT CCCCCAGCTC CAGCGTGCCC
    AGTTCTCCTG CCGGCTCTGG GCACACACGG CCGAGCTCCC TCCACGGTCT GGCGCCCAAG
    CTCCAACGCC AGTACCGCTC TCCACGGCGC AAGTCAGCAG GCAGCATCCC GCTGTCACCA
    CTGGCCCACA CCCCTTCACC ACCACCAGCG GCTTCGCCTC AGCGTTCCCC ATCACCTCTG
    TCTGGCCACG GGGCCCAGGC CTTCCCTACC AAGCTCCACT TGTCGCCTCC CCTAGGCCGG
    CAACTCTCAC GGCCCAAGAG CGCGGAGCCA CCCCGCTCAC CACTACTTAA GAGGGTGCAG
    TCAGCTGAAA AACTGGCAGC TGCACTGGCT GCTTCTGAGA AGAAGCTCGC CACTTCTCGA
    AAACAGAGCC TTGACCTGCC CCACCCCGAG CTAAAGAAGG AATTGCCGCC CAGGGAAGTC
    AGCTCTCTGG AGGTAGTTGG GACCAGGAGT GTGCTGTCTG GAAAGGGGGC GCTGCCAGGG
    AAAGGGGTGT TGCAGGCTGC TCCCTCACGG GCCTTAGGGA CCCTCCGCCA GGACAGGGCT
    GAACGGCGAG AGTCGCTGCA GAAGCAGGAA GCCATCCGGG AGGTTGACTC TTCAGAAGAT
    GATACTGATG AGGGGCCTGA GAACAGCCAA GGTGTTCAGG AGCCAAGCTT GGTACCCCAC
    CGAGAAGGGG GCCAGGATCC ACCCCTCAGA GGAGCAGGAG AGGGTGAGGA AGAGGATGCC
    TTCTTGCCCA GGGATCCCAG GGGACAGGGC CCAGTGGTCA CAGGTCTACT GACAGGGGTC
    ACACTGGGAT CTCCCAGAAT GGAAGGCCCT GGTGTCCCCC AGAAGAAGCT TGGAATCCTA
    CAGGCTTTTG AAGAGGGTTC CAGCTCCTCG TCCTCAGCCC CCAACCTGGG GAAGGCTGGC
    CCCACAGACC CCATCCCCGC TGAAGGCTGC TGGAAGGCCC AGCACCTCCA CACCCAGGCA
    CTGACAGCAC TTTGTCCGAG CTCATCAGGA CGCATCCCTG CCAGTTGCTC CACTGCCTCC
    ACCCCTGGAG AGCTGGGCCC ATGGTCCTGG AAATTCCTCT TCAAGGGCCC TGACAGGGCA
    TACCCAACCG GAAAGGCAGC AATGGAAGAT GGGCCAGCCA GCTCCCAAGA TTTGGAAACT
    ACAACTCCAG GCCACCCTGC AAACCTGTCC CCCAGACAGG AGGGGAAGTC ATGGCCACCC
    AGTGCCCCTG GGCTGGCCCA TCCACCTTGT GAGGTTCCCA GCCAGAGCTG GCTATGGGAG
    TCTGAGTGTG CACAGAGAGA GAAAGAGGAG CCAGCGCTGG GCATCACCAA AGTGCCTGAT
    GCCTCAGGTG ACAGGAGGCA GGACGTTCCA TGCAAGAGCT GCCCCTTCAC CCCAGAGCCT
    GGGCCCAGCC TGCTCCAGAA GGGCCAAGAC CCAGGGGGCC CTCAGGAGCA TCAGGACTTG
    GCATTGTTAC CTGATGAGCT TTTAAAGCAA ACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318275.1
    CDS267..5402
    Translation

    Target ORF information:

    RefSeq Version XM_015462789.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462789.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
    CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG
    TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT
    TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG
    TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA
    CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC
    CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGGACTGAT
    GGACGGCGCT GGTCTTTGGC CTCATTGCCT TCTTCAGGCT ATGGAACCAA CACCCCTAGT
    TCTACCGTGT CATCGTCGTG CTCCTCTCAG GAGAAGCTGC ACCAGTTGCC TTTCCAGCCC
    ACGGCCGATG AGCTGCACTT CTTGACGAAG CATTTCAGCA CAGACAGTGT GCCTGATGAG
    GAGGGGCGGC AGTCCCCCGC CCTGCGGCCC CGCTCCCGCA GCCTCAGTCC TGGACGGTCA
    CCAGTTTCCT TTGACAGTGA AATAATAATG ATGAATCATG TATACAAGGA AAGATTCCCC
    AAGGCCACTG CACAAATGGA AGAGCGTCTG GCCGAGTTCA TCTCCTCCAA TACTCCAGAC
    AACGTGTTGC CCTTGGCTGA TGGAACCTTG AGCTTTATTC ATCATCAGGT GATAGAGATG
    GCCCGAGACT GCCTGGACAA ATCCCGGAGT GGCCTCATTA CCTCACACTA CTTCTACGAA
    CTTCAAGAGA ATTTAGAGAA GCTTCTGCAA GATGCTCATG AGCGCTCAGA GAGTTCAGAA
    GTGGCTTTTG TGATGCAGCT GGTGAAAAAG CTGATGATTG TCATTGCCCG CCCAGCTCGT
    CTCCTGGAAT GCCTGGAGTT TGACCCTGAA GAGTTCTACC ACCTGTTAGA AGCAGCTGAG
    GGCCACGCCA AAGAGGGACA AGGGATTAAA TGTGACATTC CCCGCTACAT CGTTAGCCAA
    CTGGGCCTCA CACGGGATCC CCTAGAGGAA ATGGCCCAGT TGAGCAGCTA TGACAGTCCT
    GATACTCCGG AGACAGATGA TTCAGTCGAG GGCCGTGGGG CATCTCTGAC ATCGAAAAAG
    ACACCCTCTG AGGAGGACTT CGAAACCATT AAGCTCATCA GCAATGGCGC CTATGGGGCC
    GTGTTCCTGG TGCGGCACAA GTCCACCCGG CAGCGCTTTG CCATGAAGAA GATCAACAAG
    CAGAACCTGA TCCTGCGGAA CCAGATCCAG CAGGCCTTCG TGGAGCGGGA CATCCTGACT
    TTTGCTGAGA ACCCCTTTGT GGTCAGCATG TTCTGCTCCT TTGAAACCAA GCGCCACCTG
    TGCATGGTGA TGGAGTATGT GGAAGGAGGA GACTGTGCCA CGCTGCTGAA GAACATCGGG
    GCCCTGCCTG TGGACATGGT GCGCCTGTAC TTTGCAGAAA CTGTGCTAGC CCTGGAGTAC
    TTACACAATT ACGGCATCGT GCACCGTGAC CTCAAGCCTG ACAACCTGCT GATTACATCC
    ATGGGTCACA TCAAGCTCAC TGATTTTGGC CTTTCCAAAA TTGGCCTTAT GAGTCTGACC
    ACAAACTTGT ACGAGGGCCA CATTGAAAAG GATGCACGGG AGTTCCTGGA CAAGCAGGTA
    TGCGGGACCC CGGAGTACAT TGCACCTGAG GTGATCCTGC GGCAGGGCTA CGGGAAGCCA
    GTGGACTGGT GGGCTATGGG CATCATCCTG TACGAGTTCC TGGTGGGCTG CGTCCCTTTC
    TTTGGAGACA CTCCGGAGGA GCTCTTTGGG CAAGTGATCA GTGATGAGAT TGTGTGGCCT
    GAGGGGGACG ATGCACTGCC GCCGGACGCC CAAGATCTCA CCTCAAAACT GCTCCACCAG
    AACCCATTGG AGAGACTGGG CACAGGCAGT GCCTATGAGG TGAAGCAGCA CCCATTCTTC
    ACTGGTCTGG ACTGGACGGG ACTTCTTCGC CAGAAGGCTG AATTTATTCC TCAGCTGGAG
    TCAGAGGATG ATACCAGCTA CTTTGACACC CGCTCAGAGC GATACCACCA CGTGGACTCG
    GAGGATGAGG AGGAAGTGAG TGAGGATGGC TGCCTTGAGA TTCGCCAGTT CTCGTCCTGT
    TCTCCAAGGT TCAGCAAGGT GTACAGCAGC ATGGAGCGGC TCTCGCTGCT CGAGGAGCGC
    CGGACGCCAC CCCCCACCAA GCGCAGCCTG AGTGAGGAAA AGGAGGACCG CACGGATGGC
    CTGGCGGGGC TGAGGGGCCG AGAGCGAAGC TGGGTGATCG GCTCCCCTGA GATATTACGG
    AAGCGGCTGT CGGTGTCCGA GTCGTCCCAC ACGGAAAGCG ATTCCAGCCC TCCACTCACC
    GTGCGGCGCC ACTGCTCGGG CCTCCTGGAC GTGCCTCGCT TCCCTGAGAG CACTGAGGAG
    GCTGTTGGCG CTCCCAGGAG GCAGCAGCAG GAGGGTGCAT GGCTTCTGAC CCCGTCTGGA
    GAGGGGGCGT CTGGGCCTGC CCTGGAACGG CCGGCCGAGC GGCGGCTGAA GCTGAATGAG
    GAACCTGCTG GCCAGAGCAG CGCCCCCAGC CCAGCCATGG AGACCCGAGG AGGCCGCGGA
    ACCCCACAGC TGGCAGAAGG AGCCACAGCC AGGGCCATCA GCGACCTGGC GGTGCGCAGG
    GCCCGCCACC GGCTGCTCTC TGGGGACTCC ATAGAAAAGC GCACCGCTCG CCCCATCAAC
    AAAGTGATCA AGTCCGCCTC AGCCACAGCC CTGTCCCTTC TCATTCCTGC TGAGCACCAC
    TCCTGCTCTC CACTGGCCAG CCCCATGTCC CCACATTCCC AGTCATCCAA CCCATCATCC
    AGGGACTCTT CTCCAAGCAG GGACTTCCTG CCAGCCCTCA GCAGCTCACG GCCCCCCATT
    GTCATCCACC GAGCCGGCAA GAAGTATGGC TTCACCCTAC GAGCCATCCG TGTCTACATG
    GGTGACTCAG ATGTCTACAC CGTGCACCAC ATGGTGTGGC ACGTGGAGGA CGGAGGTCCA
    GCCAGTGAGG CAGGGCTGCG TCAGGGGGAC CTCATCACGC ACGTCAACGG GGAGCCTGTG
    CACGGGCTGG TGCACACGGA GGTGGTGGAG CTGATCCTGA AGAGTGGAAA CAAGGTGTCT
    ATTTCAACAA CTCCCCTGGA GAACACCTCC ATTAAGGTGG GGCCCGCTCG GAAGGGCAGC
    TACAAGGCCA AGATGGCCCG AAGGAGCAAG CGGAGCCGGG GCAAGGATGG ACAAGAAAGC
    AGAAAAAGGA GCTCCCTGTT CCGGAAGATC ACCAAGCAGG CATCCCTGCT CCACACCAGC
    CGCAGCCTTT CTTCCCTTAA CCGCTCCCTG TCGTCCGGCG AGAGTGGGCC AGGCTCGCCC
    ACACACAGCC ACAGCCTTTC TCCCCGCTCT CCCACGCAGG GCTACCGGGT GACCCCTGAA
    GCCGTGCACT CAGTGGGGGG GAATTCATCA CAGAGCAGCT CCCCCAGCTC CAGCGTGCCC
    AGTTCTCCTG CCGGCTCTGG GCACACACGG CCGAGCTCCC TCCACGGTCT GGCGCCCAAG
    CTCCAACGCC AGTACCGCTC TCCACGGCGC AAGTCAGCAG GCAGCATCCC GCTGTCACCA
    CTGGCCCACA CCCCTTCACC ACCACCAGCG GCTTCGCCTC AGCGTTCCCC ATCACCTCTG
    TCTGGCCACG GGGCCCAGGC CTTCCCTACC AAGCTCCACT TGTCGCCTCC CCTAGGCCGG
    CAACTCTCAC GGCCCAAGAG CGCGGAGCCA CCCCGCTCAC CACTACTTAA GAGGGTGCAG
    TCAGCTGAAA AACTGGCAGC TGCACTGGCT GCTTCTGAGA AGAAGCTCGC CACTTCTCGA
    AAACAGAGCC TTGACCTGCC CCACCCCGAG CTAAAGAAGG AATTGCCGCC CAGGGAAGTC
    AGCTCTCTGG AGGTAGTTGG GACCAGGAGT GTGCTGTCTG GAAAGGGGGC GCTGCCAGGG
    AAAGGGGTGT TGCAGGCTGC TCCCTCACGG GCCTTAGGGA CCCTCCGCCA GGACAGGGCT
    GAACGGCGAG AGTCGCTGCA GAAGCAGGAA GCCATCCGGG AGGTTGACTC TTCAGAAGAT
    GATACTGATG AGGGGCCTGA GAACAGCCAA GGTGTTCAGG AGCCAAGCTT GGTACCCCAC
    CGAGAAGGGG GCCAGGATCC ACCCCTCAGA GGAGCAGGAG AGGGTGAGGA AGAGGATGCC
    TTCTTGCCCA GGGATCCCAG GGGACAGGGC CCAGTGGTCA CAGGTCTACT GACAGGGGTC
    ACACTGGGAT CTCCCAGAAT GGAAGGCCCT GGTGTCCCCC AGAAGAAGCT TGGAATCCTA
    CAGGCTTTTG AAGAGGGTTC CAGCTCCTCG TCCTCAGCCC CCAACCTGGG GAAGGCTGGC
    CCCACAGACC CCATCCCCGC TGAAGGCTGC TGGAAGGCCC AGCACCTCCA CACCCAGGCA
    CTGACAGCAC TTTGTCCGAG CTCATCAGGA CGCATCCCTG CCAGTTGCTC CACTGCCTCC
    ACCCCTGGAG AGCTGGGCCC ATGGTCCTGG AAATTCCTCT TCAAGGGCCC TGACAGGGCA
    TACCCAACCG GAAAGGCAGC AATGGAAGAT GGGCCAGCCA GCTCCCAAGA TTTGGAAACT
    ACAACTCCAG GCCACCCTGC AAACCTGTCC CCCAGACAGG AGGGGAAGTC ATGGCCACCC
    AGTGCCCCTG GGCTGGCCCA TCCACCTTGT GAGGTTCCCA GCCAGAGCTG GCTATGGGAG
    TCTGAGTGTG CACAGAGAGA GAAAGAGGAG CCAGCGCTGG GCATCACCAA AGTGCCTGAT
    GCCTCAGGTG ACAGGAGGCA GGACGTTCCA TGCAAGAGCT GCCCCTTCAC CCCAGAGCCT
    GGGCCCAGCC TGCTCCAGAA GGGCCAAGAC CCAGGGGGCC CTCAGGAGCA TCAGGACTTG
    GCATTGTTAC CTGATGAGCT TTTAAAGCAA ACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18163
    Clone ID Related Accession (Same CDS sequence) XM_015462789.1 , XM_005204884.3
    Accession Version XM_005204884.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5136bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204884.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
    CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG
    TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT
    TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG
    TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA
    CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC
    CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGGACTGAT
    GGACGGCGCT GGTCTTTGGC CTCATTGCCT TCTTCAGGCT ATGGAACCAA CACCCCTAGT
    TCTACCGTGT CATCGTCGTG CTCCTCTCAG GAGAAGCTGC ACCAGTTGCC TTTCCAGCCC
    ACGGCCGATG AGCTGCACTT CTTGACGAAG CATTTCAGCA CAGACAGTGT GCCTGATGAG
    GAGGGGCGGC AGTCCCCCGC CCTGCGGCCC CGCTCCCGCA GCCTCAGTCC TGGACGGTCA
    CCAGTTTCCT TTGACAGTGA AATAATAATG ATGAATCATG TATACAAGGA AAGATTCCCC
    AAGGCCACTG CACAAATGGA AGAGCGTCTG GCCGAGTTCA TCTCCTCCAA TACTCCAGAC
    AACGTGTTGC CCTTGGCTGA TGGAACCTTG AGCTTTATTC ATCATCAGGT GATAGAGATG
    GCCCGAGACT GCCTGGACAA ATCCCGGAGT GGCCTCATTA CCTCACACTA CTTCTACGAA
    CTTCAAGAGA ATTTAGAGAA GCTTCTGCAA GATGCTCATG AGCGCTCAGA GAGTTCAGAA
    GTGGCTTTTG TGATGCAGCT GGTGAAAAAG CTGATGATTG TCATTGCCCG CCCAGCTCGT
    CTCCTGGAAT GCCTGGAGTT TGACCCTGAA GAGTTCTACC ACCTGTTAGA AGCAGCTGAG
    GGCCACGCCA AAGAGGGACA AGGGATTAAA TGTGACATTC CCCGCTACAT CGTTAGCCAA
    CTGGGCCTCA CACGGGATCC CCTAGAGGAA ATGGCCCAGT TGAGCAGCTA TGACAGTCCT
    GATACTCCGG AGACAGATGA TTCAGTCGAG GGCCGTGGGG CATCTCTGAC ATCGAAAAAG
    ACACCCTCTG AGGAGGACTT CGAAACCATT AAGCTCATCA GCAATGGCGC CTATGGGGCC
    GTGTTCCTGG TGCGGCACAA GTCCACCCGG CAGCGCTTTG CCATGAAGAA GATCAACAAG
    CAGAACCTGA TCCTGCGGAA CCAGATCCAG CAGGCCTTCG TGGAGCGGGA CATCCTGACT
    TTTGCTGAGA ACCCCTTTGT GGTCAGCATG TTCTGCTCCT TTGAAACCAA GCGCCACCTG
    TGCATGGTGA TGGAGTATGT GGAAGGAGGA GACTGTGCCA CGCTGCTGAA GAACATCGGG
    GCCCTGCCTG TGGACATGGT GCGCCTGTAC TTTGCAGAAA CTGTGCTAGC CCTGGAGTAC
    TTACACAATT ACGGCATCGT GCACCGTGAC CTCAAGCCTG ACAACCTGCT GATTACATCC
    ATGGGTCACA TCAAGCTCAC TGATTTTGGC CTTTCCAAAA TTGGCCTTAT GAGTCTGACC
    ACAAACTTGT ACGAGGGCCA CATTGAAAAG GATGCACGGG AGTTCCTGGA CAAGCAGGTA
    TGCGGGACCC CGGAGTACAT TGCACCTGAG GTGATCCTGC GGCAGGGCTA CGGGAAGCCA
    GTGGACTGGT GGGCTATGGG CATCATCCTG TACGAGTTCC TGGTGGGCTG CGTCCCTTTC
    TTTGGAGACA CTCCGGAGGA GCTCTTTGGG CAAGTGATCA GTGATGAGAT TGTGTGGCCT
    GAGGGGGACG ATGCACTGCC GCCGGACGCC CAAGATCTCA CCTCAAAACT GCTCCACCAG
    AACCCATTGG AGAGACTGGG CACAGGCAGT GCCTATGAGG TGAAGCAGCA CCCATTCTTC
    ACTGGTCTGG ACTGGACGGG ACTTCTTCGC CAGAAGGCTG AATTTATTCC TCAGCTGGAG
    TCAGAGGATG ATACCAGCTA CTTTGACACC CGCTCAGAGC GATACCACCA CGTGGACTCG
    GAGGATGAGG AGGAAGTGAG TGAGGATGGC TGCCTTGAGA TTCGCCAGTT CTCGTCCTGT
    TCTCCAAGGT TCAGCAAGGT GTACAGCAGC ATGGAGCGGC TCTCGCTGCT CGAGGAGCGC
    CGGACGCCAC CCCCCACCAA GCGCAGCCTG AGTGAGGAAA AGGAGGACCG CACGGATGGC
    CTGGCGGGGC TGAGGGGCCG AGAGCGAAGC TGGGTGATCG GCTCCCCTGA GATATTACGG
    AAGCGGCTGT CGGTGTCCGA GTCGTCCCAC ACGGAAAGCG ATTCCAGCCC TCCACTCACC
    GTGCGGCGCC ACTGCTCGGG CCTCCTGGAC GTGCCTCGCT TCCCTGAGAG CACTGAGGAG
    GCTGTTGGCG CTCCCAGGAG GCAGCAGCAG GAGGGTGCAT GGCTTCTGAC CCCGTCTGGA
    GAGGGGGCGT CTGGGCCTGC CCTGGAACGG CCGGCCGAGC GGCGGCTGAA GCTGAATGAG
    GAACCTGCTG GCCAGAGCAG CGCCCCCAGC CCAGCCATGG AGACCCGAGG AGGCCGCGGA
    ACCCCACAGC TGGCAGAAGG AGCCACAGCC AGGGCCATCA GCGACCTGGC GGTGCGCAGG
    GCCCGCCACC GGCTGCTCTC TGGGGACTCC ATAGAAAAGC GCACCGCTCG CCCCATCAAC
    AAAGTGATCA AGTCCGCCTC AGCCACAGCC CTGTCCCTTC TCATTCCTGC TGAGCACCAC
    TCCTGCTCTC CACTGGCCAG CCCCATGTCC CCACATTCCC AGTCATCCAA CCCATCATCC
    AGGGACTCTT CTCCAAGCAG GGACTTCCTG CCAGCCCTCA GCAGCTCACG GCCCCCCATT
    GTCATCCACC GAGCCGGCAA GAAGTATGGC TTCACCCTAC GAGCCATCCG TGTCTACATG
    GGTGACTCAG ATGTCTACAC CGTGCACCAC ATGGTGTGGC ACGTGGAGGA CGGAGGTCCA
    GCCAGTGAGG CAGGGCTGCG TCAGGGGGAC CTCATCACGC ACGTCAACGG GGAGCCTGTG
    CACGGGCTGG TGCACACGGA GGTGGTGGAG CTGATCCTGA AGAGTGGAAA CAAGGTGTCT
    ATTTCAACAA CTCCCCTGGA GAACACCTCC ATTAAGGTGG GGCCCGCTCG GAAGGGCAGC
    TACAAGGCCA AGATGGCCCG AAGGAGCAAG CGGAGCCGGG GCAAGGATGG ACAAGAAAGC
    AGAAAAAGGA GCTCCCTGTT CCGGAAGATC ACCAAGCAGG CATCCCTGCT CCACACCAGC
    CGCAGCCTTT CTTCCCTTAA CCGCTCCCTG TCGTCCGGCG AGAGTGGGCC AGGCTCGCCC
    ACACACAGCC ACAGCCTTTC TCCCCGCTCT CCCACGCAGG GCTACCGGGT GACCCCTGAA
    GCCGTGCACT CAGTGGGGGG GAATTCATCA CAGAGCAGCT CCCCCAGCTC CAGCGTGCCC
    AGTTCTCCTG CCGGCTCTGG GCACACACGG CCGAGCTCCC TCCACGGTCT GGCGCCCAAG
    CTCCAACGCC AGTACCGCTC TCCACGGCGC AAGTCAGCAG GCAGCATCCC GCTGTCACCA
    CTGGCCCACA CCCCTTCACC ACCACCAGCG GCTTCGCCTC AGCGTTCCCC ATCACCTCTG
    TCTGGCCACG GGGCCCAGGC CTTCCCTACC AAGCTCCACT TGTCGCCTCC CCTAGGCCGG
    CAACTCTCAC GGCCCAAGAG CGCGGAGCCA CCCCGCTCAC CACTACTTAA GAGGGTGCAG
    TCAGCTGAAA AACTGGCAGC TGCACTGGCT GCTTCTGAGA AGAAGCTCGC CACTTCTCGA
    AAACAGAGCC TTGACCTGCC CCACCCCGAG CTAAAGAAGG AATTGCCGCC CAGGGAAGTC
    AGCTCTCTGG AGGTAGTTGG GACCAGGAGT GTGCTGTCTG GAAAGGGGGC GCTGCCAGGG
    AAAGGGGTGT TGCAGGCTGC TCCCTCACGG GCCTTAGGGA CCCTCCGCCA GGACAGGGCT
    GAACGGCGAG AGTCGCTGCA GAAGCAGGAA GCCATCCGGG AGGTTGACTC TTCAGAAGAT
    GATACTGATG AGGGGCCTGA GAACAGCCAA GGTGTTCAGG AGCCAAGCTT GGTACCCCAC
    CGAGAAGGGG GCCAGGATCC ACCCCTCAGA GGAGCAGGAG AGGGTGAGGA AGAGGATGCC
    TTCTTGCCCA GGGATCCCAG GGGACAGGGC CCAGTGGTCA CAGGTCTACT GACAGGGGTC
    ACACTGGGAT CTCCCAGAAT GGAAGGCCCT GGTGTCCCCC AGAAGAAGCT TGGAATCCTA
    CAGGCTTTTG AAGAGGGTTC CAGCTCCTCG TCCTCAGCCC CCAACCTGGG GAAGGCTGGC
    CCCACAGACC CCATCCCCGC TGAAGGCTGC TGGAAGGCCC AGCACCTCCA CACCCAGGCA
    CTGACAGCAC TTTGTCCGAG CTCATCAGGA CGCATCCCTG CCAGTTGCTC CACTGCCTCC
    ACCCCTGGAG AGCTGGGCCC ATGGTCCTGG AAATTCCTCT TCAAGGGCCC TGACAGGGCA
    TACCCAACCG GAAAGGCAGC AATGGAAGAT GGGCCAGCCA GCTCCCAAGA TTTGGAAACT
    ACAACTCCAG GCCACCCTGC AAACCTGTCC CCCAGACAGG AGGGGAAGTC ATGGCCACCC
    AGTGCCCCTG GGCTGGCCCA TCCACCTTGT GAGGTTCCCA GCCAGAGCTG GCTATGGGAG
    TCTGAGTGTG CACAGAGAGA GAAAGAGGAG CCAGCGCTGG GCATCACCAA AGTGCCTGAT
    GCCTCAGGTG ACAGGAGGCA GGACGTTCCA TGCAAGAGCT GCCCCTTCAC CCCAGAGCCT
    GGGCCCAGCC TGCTCCAGAA GGGCCAAGAC CCAGGGGGCC CTCAGGAGCA TCAGGACTTG
    GCATTGTTAC CTGATGAGCT TTTAAAGCAA ACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005204941.1
    CDS267..5402
    Translation

    Target ORF information:

    RefSeq Version XM_005204884.3
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204884.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA AATCTCTGCT 
    CTTTGCCGAG GATCTGAAGT AAACCGGCAC GTACTTTCTC CCACGTCGGC TCCAGCCTGG
    TCCCTCTCTA AAGTCCCCTT TAGTGCCGAA TGTACTCTGG CTACTTCTCC TCTTGCCTTT
    TTCCTGAACA CACACGCCCA TAGCAGTCCT GGCAGCCCCT GTTCCAGCCG CTCACTGCAG
    TGGAGCTGTC GGACAAGTAA CCGCAAGAGT TTGATTGTGA CCTCTAGTAC GTCACCTACA
    CTGCCACGGC CACACTCACC ACTCCATGGC CACACAGGTA ACAGTCCTTT GGATAGTCCC
    CGGAATTTCT CTCCAAATGC ACCTGCTCAC TTTTCCTTTG TTCCTGCCCG TAGGACTGAT
    GGACGGCGCT GGTCTTTGGC CTCATTGCCT TCTTCAGGCT ATGGAACCAA CACCCCTAGT
    TCTACCGTGT CATCGTCGTG CTCCTCTCAG GAGAAGCTGC ACCAGTTGCC TTTCCAGCCC
    ACGGCCGATG AGCTGCACTT CTTGACGAAG CATTTCAGCA CAGACAGTGT GCCTGATGAG
    GAGGGGCGGC AGTCCCCCGC CCTGCGGCCC CGCTCCCGCA GCCTCAGTCC TGGACGGTCA
    CCAGTTTCCT TTGACAGTGA AATAATAATG ATGAATCATG TATACAAGGA AAGATTCCCC
    AAGGCCACTG CACAAATGGA AGAGCGTCTG GCCGAGTTCA TCTCCTCCAA TACTCCAGAC
    AACGTGTTGC CCTTGGCTGA TGGAACCTTG AGCTTTATTC ATCATCAGGT GATAGAGATG
    GCCCGAGACT GCCTGGACAA ATCCCGGAGT GGCCTCATTA CCTCACACTA CTTCTACGAA
    CTTCAAGAGA ATTTAGAGAA GCTTCTGCAA GATGCTCATG AGCGCTCAGA GAGTTCAGAA
    GTGGCTTTTG TGATGCAGCT GGTGAAAAAG CTGATGATTG TCATTGCCCG CCCAGCTCGT
    CTCCTGGAAT GCCTGGAGTT TGACCCTGAA GAGTTCTACC ACCTGTTAGA AGCAGCTGAG
    GGCCACGCCA AAGAGGGACA AGGGATTAAA TGTGACATTC CCCGCTACAT CGTTAGCCAA
    CTGGGCCTCA CACGGGATCC CCTAGAGGAA ATGGCCCAGT TGAGCAGCTA TGACAGTCCT
    GATACTCCGG AGACAGATGA TTCAGTCGAG GGCCGTGGGG CATCTCTGAC ATCGAAAAAG
    ACACCCTCTG AGGAGGACTT CGAAACCATT AAGCTCATCA GCAATGGCGC CTATGGGGCC
    GTGTTCCTGG TGCGGCACAA GTCCACCCGG CAGCGCTTTG CCATGAAGAA GATCAACAAG
    CAGAACCTGA TCCTGCGGAA CCAGATCCAG CAGGCCTTCG TGGAGCGGGA CATCCTGACT
    TTTGCTGAGA ACCCCTTTGT GGTCAGCATG TTCTGCTCCT TTGAAACCAA GCGCCACCTG
    TGCATGGTGA TGGAGTATGT GGAAGGAGGA GACTGTGCCA CGCTGCTGAA GAACATCGGG
    GCCCTGCCTG TGGACATGGT GCGCCTGTAC TTTGCAGAAA CTGTGCTAGC CCTGGAGTAC
    TTACACAATT ACGGCATCGT GCACCGTGAC CTCAAGCCTG ACAACCTGCT GATTACATCC
    ATGGGTCACA TCAAGCTCAC TGATTTTGGC CTTTCCAAAA TTGGCCTTAT GAGTCTGACC
    ACAAACTTGT ACGAGGGCCA CATTGAAAAG GATGCACGGG AGTTCCTGGA CAAGCAGGTA
    TGCGGGACCC CGGAGTACAT TGCACCTGAG GTGATCCTGC GGCAGGGCTA CGGGAAGCCA
    GTGGACTGGT GGGCTATGGG CATCATCCTG TACGAGTTCC TGGTGGGCTG CGTCCCTTTC
    TTTGGAGACA CTCCGGAGGA GCTCTTTGGG CAAGTGATCA GTGATGAGAT TGTGTGGCCT
    GAGGGGGACG ATGCACTGCC GCCGGACGCC CAAGATCTCA CCTCAAAACT GCTCCACCAG
    AACCCATTGG AGAGACTGGG CACAGGCAGT GCCTATGAGG TGAAGCAGCA CCCATTCTTC
    ACTGGTCTGG ACTGGACGGG ACTTCTTCGC CAGAAGGCTG AATTTATTCC TCAGCTGGAG
    TCAGAGGATG ATACCAGCTA CTTTGACACC CGCTCAGAGC GATACCACCA CGTGGACTCG
    GAGGATGAGG AGGAAGTGAG TGAGGATGGC TGCCTTGAGA TTCGCCAGTT CTCGTCCTGT
    TCTCCAAGGT TCAGCAAGGT GTACAGCAGC ATGGAGCGGC TCTCGCTGCT CGAGGAGCGC
    CGGACGCCAC CCCCCACCAA GCGCAGCCTG AGTGAGGAAA AGGAGGACCG CACGGATGGC
    CTGGCGGGGC TGAGGGGCCG AGAGCGAAGC TGGGTGATCG GCTCCCCTGA GATATTACGG
    AAGCGGCTGT CGGTGTCCGA GTCGTCCCAC ACGGAAAGCG ATTCCAGCCC TCCACTCACC
    GTGCGGCGCC ACTGCTCGGG CCTCCTGGAC GTGCCTCGCT TCCCTGAGAG CACTGAGGAG
    GCTGTTGGCG CTCCCAGGAG GCAGCAGCAG GAGGGTGCAT GGCTTCTGAC CCCGTCTGGA
    GAGGGGGCGT CTGGGCCTGC CCTGGAACGG CCGGCCGAGC GGCGGCTGAA GCTGAATGAG
    GAACCTGCTG GCCAGAGCAG CGCCCCCAGC CCAGCCATGG AGACCCGAGG AGGCCGCGGA
    ACCCCACAGC TGGCAGAAGG AGCCACAGCC AGGGCCATCA GCGACCTGGC GGTGCGCAGG
    GCCCGCCACC GGCTGCTCTC TGGGGACTCC ATAGAAAAGC GCACCGCTCG CCCCATCAAC
    AAAGTGATCA AGTCCGCCTC AGCCACAGCC CTGTCCCTTC TCATTCCTGC TGAGCACCAC
    TCCTGCTCTC CACTGGCCAG CCCCATGTCC CCACATTCCC AGTCATCCAA CCCATCATCC
    AGGGACTCTT CTCCAAGCAG GGACTTCCTG CCAGCCCTCA GCAGCTCACG GCCCCCCATT
    GTCATCCACC GAGCCGGCAA GAAGTATGGC TTCACCCTAC GAGCCATCCG TGTCTACATG
    GGTGACTCAG ATGTCTACAC CGTGCACCAC ATGGTGTGGC ACGTGGAGGA CGGAGGTCCA
    GCCAGTGAGG CAGGGCTGCG TCAGGGGGAC CTCATCACGC ACGTCAACGG GGAGCCTGTG
    CACGGGCTGG TGCACACGGA GGTGGTGGAG CTGATCCTGA AGAGTGGAAA CAAGGTGTCT
    ATTTCAACAA CTCCCCTGGA GAACACCTCC ATTAAGGTGG GGCCCGCTCG GAAGGGCAGC
    TACAAGGCCA AGATGGCCCG AAGGAGCAAG CGGAGCCGGG GCAAGGATGG ACAAGAAAGC
    AGAAAAAGGA GCTCCCTGTT CCGGAAGATC ACCAAGCAGG CATCCCTGCT CCACACCAGC
    CGCAGCCTTT CTTCCCTTAA CCGCTCCCTG TCGTCCGGCG AGAGTGGGCC AGGCTCGCCC
    ACACACAGCC ACAGCCTTTC TCCCCGCTCT CCCACGCAGG GCTACCGGGT GACCCCTGAA
    GCCGTGCACT CAGTGGGGGG GAATTCATCA CAGAGCAGCT CCCCCAGCTC CAGCGTGCCC
    AGTTCTCCTG CCGGCTCTGG GCACACACGG CCGAGCTCCC TCCACGGTCT GGCGCCCAAG
    CTCCAACGCC AGTACCGCTC TCCACGGCGC AAGTCAGCAG GCAGCATCCC GCTGTCACCA
    CTGGCCCACA CCCCTTCACC ACCACCAGCG GCTTCGCCTC AGCGTTCCCC ATCACCTCTG
    TCTGGCCACG GGGCCCAGGC CTTCCCTACC AAGCTCCACT TGTCGCCTCC CCTAGGCCGG
    CAACTCTCAC GGCCCAAGAG CGCGGAGCCA CCCCGCTCAC CACTACTTAA GAGGGTGCAG
    TCAGCTGAAA AACTGGCAGC TGCACTGGCT GCTTCTGAGA AGAAGCTCGC CACTTCTCGA
    AAACAGAGCC TTGACCTGCC CCACCCCGAG CTAAAGAAGG AATTGCCGCC CAGGGAAGTC
    AGCTCTCTGG AGGTAGTTGG GACCAGGAGT GTGCTGTCTG GAAAGGGGGC GCTGCCAGGG
    AAAGGGGTGT TGCAGGCTGC TCCCTCACGG GCCTTAGGGA CCCTCCGCCA GGACAGGGCT
    GAACGGCGAG AGTCGCTGCA GAAGCAGGAA GCCATCCGGG AGGTTGACTC TTCAGAAGAT
    GATACTGATG AGGGGCCTGA GAACAGCCAA GGTGTTCAGG AGCCAAGCTT GGTACCCCAC
    CGAGAAGGGG GCCAGGATCC ACCCCTCAGA GGAGCAGGAG AGGGTGAGGA AGAGGATGCC
    TTCTTGCCCA GGGATCCCAG GGGACAGGGC CCAGTGGTCA CAGGTCTACT GACAGGGGTC
    ACACTGGGAT CTCCCAGAAT GGAAGGCCCT GGTGTCCCCC AGAAGAAGCT TGGAATCCTA
    CAGGCTTTTG AAGAGGGTTC CAGCTCCTCG TCCTCAGCCC CCAACCTGGG GAAGGCTGGC
    CCCACAGACC CCATCCCCGC TGAAGGCTGC TGGAAGGCCC AGCACCTCCA CACCCAGGCA
    CTGACAGCAC TTTGTCCGAG CTCATCAGGA CGCATCCCTG CCAGTTGCTC CACTGCCTCC
    ACCCCTGGAG AGCTGGGCCC ATGGTCCTGG AAATTCCTCT TCAAGGGCCC TGACAGGGCA
    TACCCAACCG GAAAGGCAGC AATGGAAGAT GGGCCAGCCA GCTCCCAAGA TTTGGAAACT
    ACAACTCCAG GCCACCCTGC AAACCTGTCC CCCAGACAGG AGGGGAAGTC ATGGCCACCC
    AGTGCCCCTG GGCTGGCCCA TCCACCTTGT GAGGTTCCCA GCCAGAGCTG GCTATGGGAG
    TCTGAGTGTG CACAGAGAGA GAAAGAGGAG CCAGCGCTGG GCATCACCAA AGTGCCTGAT
    GCCTCAGGTG ACAGGAGGCA GGACGTTCCA TGCAAGAGCT GCCCCTTCAC CCCAGAGCCT
    GGGCCCAGCC TGCTCCAGAA GGGCCAAGAC CCAGGGGGCC CTCAGGAGCA TCAGGACTTG
    GCATTGTTAC CTGATGAGCT TTTAAAGCAA ACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18165
    Clone ID Related Accession (Same CDS sequence) XM_005204886.3 , XM_005204886.4 , XM_015462790.1
    Accession Version XM_005204886.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA CTGTCGGACA 
    AGTAACCGCA AGAGTTTGAT TGTGACCTCT AGTACGTCAC CTACACTGCC ACGGCCACAC
    TCACCACTCC ATGGCCACAC AGGTAACAGT CCTTTGGATA GTCCCCGGAA TTTCTCTCCA
    AATGCACCTG CTCACTTTTC CTTTGTTCCT GCCCGTAGCC ATGGCCACAG AGCAGACAGG
    ACTGATGGAC GGCGCTGGTC TTTGGCCTCA TTGCCTTCTT CAGGCTATGG AACCAACACC
    CCTAGTTCTA CCGTGTCATC GTCGTGCTCC TCTCAGGAGA AGCTGCACCA GTTGCCTTTC
    CAGCCCACGG CCGATGAGCT GCACTTCTTG ACGAAGCATT TCAGCACAGA CAGTGTGCCT
    GATGAGGAGG GGCGGCAGTC CCCCGCCCTG CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA
    CGGTCACCAG TTTCCTTTGA CAGTGAAATA ATAATGATGA ATCATGTATA CAAGGAAAGA
    TTCCCCAAGG CCACTGCACA AATGGAAGAG CGTCTGGCCG AGTTCATCTC CTCCAATACT
    CCAGACAACG TGTTGCCCTT GGCTGATGGA ACCTTGAGCT TTATTCATCA TCAGGTGATA
    GAGATGGCCC GAGACTGCCT GGACAAATCC CGGAGTGGCC TCATTACCTC ACACTACTTC
    TACGAACTTC AAGAGAATTT AGAGAAGCTT CTGCAAGATG CTCATGAGCG CTCAGAGAGT
    TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG AAAAAGCTGA TGATTGTCAT TGCCCGCCCA
    GCTCGTCTCC TGGAATGCCT GGAGTTTGAC CCTGAAGAGT TCTACCACCT GTTAGAAGCA
    GCTGAGGGCC ACGCCAAAGA GGGACAAGGG ATTAAATGTG ACATTCCCCG CTACATCGTT
    AGCCAACTGG GCCTCACACG GGATCCCCTA GAGGAAATGG CCCAGTTGAG CAGCTATGAC
    AGTCCTGATA CTCCGGAGAC AGATGATTCA GTCGAGGGCC GTGGGGCATC TCTGACATCG
    AAAAAGACAC CCTCTGAGGA GGACTTCGAA ACCATTAAGC TCATCAGCAA TGGCGCCTAT
    GGGGCCGTGT TCCTGGTGCG GCACAAGTCC ACCCGGCAGC GCTTTGCCAT GAAGAAGATC
    AACAAGCAGA ACCTGATCCT GCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGGGACATC
    CTGACTTTTG CTGAGAACCC CTTTGTGGTC AGCATGTTCT GCTCCTTTGA AACCAAGCGC
    CACCTGTGCA TGGTGATGGA GTATGTGGAA GGAGGAGACT GTGCCACGCT GCTGAAGAAC
    ATCGGGGCCC TGCCTGTGGA CATGGTGCGC CTGTACTTTG CAGAAACTGT GCTAGCCCTG
    GAGTACTTAC ACAATTACGG CATCGTGCAC CGTGACCTCA AGCCTGACAA CCTGCTGATT
    ACATCCATGG GTCACATCAA GCTCACTGAT TTTGGCCTTT CCAAAATTGG CCTTATGAGT
    CTGACCACAA ACTTGTACGA GGGCCACATT GAAAAGGATG CACGGGAGTT CCTGGACAAG
    CAGGTATGCG GGACCCCGGA GTACATTGCA CCTGAGGTGA TCCTGCGGCA GGGCTACGGG
    AAGCCAGTGG ACTGGTGGGC TATGGGCATC ATCCTGTACG AGTTCCTGGT GGGCTGCGTC
    CCTTTCTTTG GAGACACTCC GGAGGAGCTC TTTGGGCAAG TGATCAGTGA TGAGATTGTG
    TGGCCTGAGG GGGACGATGC ACTGCCGCCG GACGCCCAAG ATCTCACCTC AAAACTGCTC
    CACCAGAACC CATTGGAGAG ACTGGGCACA GGCAGTGCCT ATGAGGTGAA GCAGCACCCA
    TTCTTCACTG GTCTGGACTG GACGGGACTT CTTCGCCAGA AGGCTGAATT TATTCCTCAG
    CTGGAGTCAG AGGATGATAC CAGCTACTTT GACACCCGCT CAGAGCGATA CCACCACGTG
    GACTCGGAGG ATGAGGAGGA AGTGAGTGAG GATGGCTGCC TTGAGATTCG CCAGTTCTCG
    TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC AGCAGCATGG AGCGGCTCTC GCTGCTCGAG
    GAGCGCCGGA CGCCACCCCC CACCAAGCGC AGCCTGAGTG AGGAAAAGGA GGACCGCACG
    GATGGCCTGG CGGGGCTGAG GGGCCGAGAG CGAAGCTGGG TGATCGGCTC CCCTGAGATA
    TTACGGAAGC GGCTGTCGGT GTCCGAGTCG TCCCACACGG AAAGCGATTC CAGCCCTCCA
    CTCACCGTGC GGCGCCACTG CTCGGGCCTC CTGGACGTGC CTCGCTTCCC TGAGAGCACT
    GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG CAGCAGGAGG GTGCATGGCT TCTGACCCCG
    TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG GAACGGCCGG CCGAGCGGCG GCTGAAGCTG
    AATGAGGAAC CTGCTGGCCA GAGCAGCGCC CCCAGCCCAG CCATGGAGAC CCGAGGAGGC
    CGCGGAACCC CACAGCTGGC AGAAGGAGCC ACAGCCAGGG CCATCAGCGA CCTGGCGGTG
    CGCAGGGCCC GCCACCGGCT GCTCTCTGGG GACTCCATAG AAAAGCGCAC CGCTCGCCCC
    ATCAACAAAG TGATCAAGTC CGCCTCAGCC ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG
    CACCACTCCT GCTCTCCACT GGCCAGCCCC ATGTCCCCAC ATTCCCAGTC ATCCAACCCA
    TCATCCAGGG ACTCTTCTCC AAGCAGGGAC TTCCTGCCAG CCCTCAGCAG CTCACGGCCC
    CCCATTGTCA TCCACCGAGC CGGCAAGAAG TATGGCTTCA CCCTACGAGC CATCCGTGTC
    TACATGGGTG ACTCAGATGT CTACACCGTG CACCACATGG TGTGGCACGT GGAGGACGGA
    GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG GGGGACCTCA TCACGCACGT CAACGGGGAG
    CCTGTGCACG GGCTGGTGCA CACGGAGGTG GTGGAGCTGA TCCTGAAGAG TGGAAACAAG
    GTGTCTATTT CAACAACTCC CCTGGAGAAC ACCTCCATTA AGGTGGGGCC CGCTCGGAAG
    GGCAGCTACA AGGCCAAGAT GGCCCGAAGG AGCAAGCGGA GCCGGGGCAA GGATGGACAA
    GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG AAGATCACCA AGCAGGCATC CCTGCTCCAC
    ACCAGCCGCA GCCTTTCTTC CCTTAACCGC TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC
    TCGCCCACAC ACAGCCACAG CCTTTCTCCC CGCTCTCCCA CGCAGGGCTA CCGGGTGACC
    CCTGAAGCCG TGCACTCAGT GGGGGGGAAT TCATCACAGA GCAGCTCCCC CAGCTCCAGC
    GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC ACACGGCCGA GCTCCCTCCA CGGTCTGGCG
    CCCAAGCTCC AACGCCAGTA CCGCTCTCCA CGGCGCAAGT CAGCAGGCAG CATCCCGCTG
    TCACCACTGG CCCACACCCC TTCACCACCA CCAGCGGCTT CGCCTCAGCG TTCCCCATCA
    CCTCTGTCTG GCCACGGGGC CCAGGCCTTC CCTACCAAGC TCCACTTGTC GCCTCCCCTA
    GGCCGGCAAC TCTCACGGCC CAAGAGCGCG GAGCCACCCC GCTCACCACT ACTTAAGAGG
    GTGCAGTCAG CTGAAAAACT GGCAGCTGCA CTGGCTGCTT CTGAGAAGAA GCTCGCCACT
    TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC CCCGAGCTAA AGAAGGAATT GCCGCCCAGG
    GAAGTCAGCT CTCTGGAGGT AGTTGGGACC AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG
    CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC TCACGGGCCT TAGGGACCCT CCGCCAGGAC
    AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG CAGGAAGCCA TCCGGGAGGT TGACTCTTCA
    GAAGATGATA CTGATGAGGG GCCTGAGAAC AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA
    CCCCACCGAG AAGGGGGCCA GGATCCACCC CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG
    GATGCCTTCT TGCCCAGGGA TCCCAGGGGA CAGGGCCCAG TGGTCACAGG TCTACTGACA
    GGGGTCACAC TGGGATCTCC CAGAATGGAA GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA
    ATCCTACAGG CTTTTGAAGA GGGTTCCAGC TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG
    GCTGGCCCCA CAGACCCCAT CCCCGCTGAA GGCTGCTGGA AGGCCCAGCA CCTCCACACC
    CAGGCACTGA CAGCACTTTG TCCGAGCTCA TCAGGACGCA TCCCTGCCAG TTGCTCCACT
    GCCTCCACCC CTGGAGAGCT GGGCCCATGG TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC
    AGGGCATACC CAACCGGAAA GGCAGCAATG GAAGATGGGC CAGCCAGCTC CCAAGATTTG
    GAAACTACAA CTCCAGGCCA CCCTGCAAAC CTGTCCCCCA GACAGGAGGG GAAGTCATGG
    CCACCCAGTG CCCCTGGGCT GGCCCATCCA CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA
    TGGGAGTCTG AGTGTGCACA GAGAGAGAAA GAGGAGCCAG CGCTGGGCAT CACCAAAGTG
    CCTGATGCCT CAGGTGACAG GAGGCAGGAC GTTCCATGCA AGAGCTGCCC CTTCACCCCA
    GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC CAAGACCCAG GGGGCCCTCA GGAGCATCAG
    GACTTGGCAT TGTTACCTGA TGAGCTTTTA AAGCAAACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005204943.1
    CDS263..5224
    Translation

    Target ORF information:

    RefSeq Version XM_005204886.3
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204886.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA CTGTCGGACA 
    AGTAACCGCA AGAGTTTGAT TGTGACCTCT AGTACGTCAC CTACACTGCC ACGGCCACAC
    TCACCACTCC ATGGCCACAC AGGTAACAGT CCTTTGGATA GTCCCCGGAA TTTCTCTCCA
    AATGCACCTG CTCACTTTTC CTTTGTTCCT GCCCGTAGCC ATGGCCACAG AGCAGACAGG
    ACTGATGGAC GGCGCTGGTC TTTGGCCTCA TTGCCTTCTT CAGGCTATGG AACCAACACC
    CCTAGTTCTA CCGTGTCATC GTCGTGCTCC TCTCAGGAGA AGCTGCACCA GTTGCCTTTC
    CAGCCCACGG CCGATGAGCT GCACTTCTTG ACGAAGCATT TCAGCACAGA CAGTGTGCCT
    GATGAGGAGG GGCGGCAGTC CCCCGCCCTG CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA
    CGGTCACCAG TTTCCTTTGA CAGTGAAATA ATAATGATGA ATCATGTATA CAAGGAAAGA
    TTCCCCAAGG CCACTGCACA AATGGAAGAG CGTCTGGCCG AGTTCATCTC CTCCAATACT
    CCAGACAACG TGTTGCCCTT GGCTGATGGA ACCTTGAGCT TTATTCATCA TCAGGTGATA
    GAGATGGCCC GAGACTGCCT GGACAAATCC CGGAGTGGCC TCATTACCTC ACACTACTTC
    TACGAACTTC AAGAGAATTT AGAGAAGCTT CTGCAAGATG CTCATGAGCG CTCAGAGAGT
    TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG AAAAAGCTGA TGATTGTCAT TGCCCGCCCA
    GCTCGTCTCC TGGAATGCCT GGAGTTTGAC CCTGAAGAGT TCTACCACCT GTTAGAAGCA
    GCTGAGGGCC ACGCCAAAGA GGGACAAGGG ATTAAATGTG ACATTCCCCG CTACATCGTT
    AGCCAACTGG GCCTCACACG GGATCCCCTA GAGGAAATGG CCCAGTTGAG CAGCTATGAC
    AGTCCTGATA CTCCGGAGAC AGATGATTCA GTCGAGGGCC GTGGGGCATC TCTGACATCG
    AAAAAGACAC CCTCTGAGGA GGACTTCGAA ACCATTAAGC TCATCAGCAA TGGCGCCTAT
    GGGGCCGTGT TCCTGGTGCG GCACAAGTCC ACCCGGCAGC GCTTTGCCAT GAAGAAGATC
    AACAAGCAGA ACCTGATCCT GCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGGGACATC
    CTGACTTTTG CTGAGAACCC CTTTGTGGTC AGCATGTTCT GCTCCTTTGA AACCAAGCGC
    CACCTGTGCA TGGTGATGGA GTATGTGGAA GGAGGAGACT GTGCCACGCT GCTGAAGAAC
    ATCGGGGCCC TGCCTGTGGA CATGGTGCGC CTGTACTTTG CAGAAACTGT GCTAGCCCTG
    GAGTACTTAC ACAATTACGG CATCGTGCAC CGTGACCTCA AGCCTGACAA CCTGCTGATT
    ACATCCATGG GTCACATCAA GCTCACTGAT TTTGGCCTTT CCAAAATTGG CCTTATGAGT
    CTGACCACAA ACTTGTACGA GGGCCACATT GAAAAGGATG CACGGGAGTT CCTGGACAAG
    CAGGTATGCG GGACCCCGGA GTACATTGCA CCTGAGGTGA TCCTGCGGCA GGGCTACGGG
    AAGCCAGTGG ACTGGTGGGC TATGGGCATC ATCCTGTACG AGTTCCTGGT GGGCTGCGTC
    CCTTTCTTTG GAGACACTCC GGAGGAGCTC TTTGGGCAAG TGATCAGTGA TGAGATTGTG
    TGGCCTGAGG GGGACGATGC ACTGCCGCCG GACGCCCAAG ATCTCACCTC AAAACTGCTC
    CACCAGAACC CATTGGAGAG ACTGGGCACA GGCAGTGCCT ATGAGGTGAA GCAGCACCCA
    TTCTTCACTG GTCTGGACTG GACGGGACTT CTTCGCCAGA AGGCTGAATT TATTCCTCAG
    CTGGAGTCAG AGGATGATAC CAGCTACTTT GACACCCGCT CAGAGCGATA CCACCACGTG
    GACTCGGAGG ATGAGGAGGA AGTGAGTGAG GATGGCTGCC TTGAGATTCG CCAGTTCTCG
    TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC AGCAGCATGG AGCGGCTCTC GCTGCTCGAG
    GAGCGCCGGA CGCCACCCCC CACCAAGCGC AGCCTGAGTG AGGAAAAGGA GGACCGCACG
    GATGGCCTGG CGGGGCTGAG GGGCCGAGAG CGAAGCTGGG TGATCGGCTC CCCTGAGATA
    TTACGGAAGC GGCTGTCGGT GTCCGAGTCG TCCCACACGG AAAGCGATTC CAGCCCTCCA
    CTCACCGTGC GGCGCCACTG CTCGGGCCTC CTGGACGTGC CTCGCTTCCC TGAGAGCACT
    GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG CAGCAGGAGG GTGCATGGCT TCTGACCCCG
    TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG GAACGGCCGG CCGAGCGGCG GCTGAAGCTG
    AATGAGGAAC CTGCTGGCCA GAGCAGCGCC CCCAGCCCAG CCATGGAGAC CCGAGGAGGC
    CGCGGAACCC CACAGCTGGC AGAAGGAGCC ACAGCCAGGG CCATCAGCGA CCTGGCGGTG
    CGCAGGGCCC GCCACCGGCT GCTCTCTGGG GACTCCATAG AAAAGCGCAC CGCTCGCCCC
    ATCAACAAAG TGATCAAGTC CGCCTCAGCC ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG
    CACCACTCCT GCTCTCCACT GGCCAGCCCC ATGTCCCCAC ATTCCCAGTC ATCCAACCCA
    TCATCCAGGG ACTCTTCTCC AAGCAGGGAC TTCCTGCCAG CCCTCAGCAG CTCACGGCCC
    CCCATTGTCA TCCACCGAGC CGGCAAGAAG TATGGCTTCA CCCTACGAGC CATCCGTGTC
    TACATGGGTG ACTCAGATGT CTACACCGTG CACCACATGG TGTGGCACGT GGAGGACGGA
    GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG GGGGACCTCA TCACGCACGT CAACGGGGAG
    CCTGTGCACG GGCTGGTGCA CACGGAGGTG GTGGAGCTGA TCCTGAAGAG TGGAAACAAG
    GTGTCTATTT CAACAACTCC CCTGGAGAAC ACCTCCATTA AGGTGGGGCC CGCTCGGAAG
    GGCAGCTACA AGGCCAAGAT GGCCCGAAGG AGCAAGCGGA GCCGGGGCAA GGATGGACAA
    GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG AAGATCACCA AGCAGGCATC CCTGCTCCAC
    ACCAGCCGCA GCCTTTCTTC CCTTAACCGC TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC
    TCGCCCACAC ACAGCCACAG CCTTTCTCCC CGCTCTCCCA CGCAGGGCTA CCGGGTGACC
    CCTGAAGCCG TGCACTCAGT GGGGGGGAAT TCATCACAGA GCAGCTCCCC CAGCTCCAGC
    GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC ACACGGCCGA GCTCCCTCCA CGGTCTGGCG
    CCCAAGCTCC AACGCCAGTA CCGCTCTCCA CGGCGCAAGT CAGCAGGCAG CATCCCGCTG
    TCACCACTGG CCCACACCCC TTCACCACCA CCAGCGGCTT CGCCTCAGCG TTCCCCATCA
    CCTCTGTCTG GCCACGGGGC CCAGGCCTTC CCTACCAAGC TCCACTTGTC GCCTCCCCTA
    GGCCGGCAAC TCTCACGGCC CAAGAGCGCG GAGCCACCCC GCTCACCACT ACTTAAGAGG
    GTGCAGTCAG CTGAAAAACT GGCAGCTGCA CTGGCTGCTT CTGAGAAGAA GCTCGCCACT
    TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC CCCGAGCTAA AGAAGGAATT GCCGCCCAGG
    GAAGTCAGCT CTCTGGAGGT AGTTGGGACC AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG
    CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC TCACGGGCCT TAGGGACCCT CCGCCAGGAC
    AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG CAGGAAGCCA TCCGGGAGGT TGACTCTTCA
    GAAGATGATA CTGATGAGGG GCCTGAGAAC AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA
    CCCCACCGAG AAGGGGGCCA GGATCCACCC CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG
    GATGCCTTCT TGCCCAGGGA TCCCAGGGGA CAGGGCCCAG TGGTCACAGG TCTACTGACA
    GGGGTCACAC TGGGATCTCC CAGAATGGAA GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA
    ATCCTACAGG CTTTTGAAGA GGGTTCCAGC TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG
    GCTGGCCCCA CAGACCCCAT CCCCGCTGAA GGCTGCTGGA AGGCCCAGCA CCTCCACACC
    CAGGCACTGA CAGCACTTTG TCCGAGCTCA TCAGGACGCA TCCCTGCCAG TTGCTCCACT
    GCCTCCACCC CTGGAGAGCT GGGCCCATGG TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC
    AGGGCATACC CAACCGGAAA GGCAGCAATG GAAGATGGGC CAGCCAGCTC CCAAGATTTG
    GAAACTACAA CTCCAGGCCA CCCTGCAAAC CTGTCCCCCA GACAGGAGGG GAAGTCATGG
    CCACCCAGTG CCCCTGGGCT GGCCCATCCA CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA
    TGGGAGTCTG AGTGTGCACA GAGAGAGAAA GAGGAGCCAG CGCTGGGCAT CACCAAAGTG
    CCTGATGCCT CAGGTGACAG GAGGCAGGAC GTTCCATGCA AGAGCTGCCC CTTCACCCCA
    GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC CAAGACCCAG GGGGCCCTCA GGAGCATCAG
    GACTTGGCAT TGTTACCTGA TGAGCTTTTA AAGCAAACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18165
    Clone ID Related Accession (Same CDS sequence) XM_005204886.3 , XM_005204886.4 , XM_015462790.1
    Accession Version XM_005204886.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204886.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA CTGTCGGACA 
    AGTAACCGCA AGAGTTTGAT TGTGACCTCT AGTACGTCAC CTACACTGCC ACGGCCACAC
    TCACCACTCC ATGGCCACAC AGGTAACAGT CCTTTGGATA GTCCCCGGAA TTTCTCTCCA
    AATGCACCTG CTCACTTTTC CTTTGTTCCT GCCCGTAGCC ATGGCCACAG AGCAGACAGG
    ACTGATGGAC GGCGCTGGTC TTTGGCCTCA TTGCCTTCTT CAGGCTATGG AACCAACACC
    CCTAGTTCTA CCGTGTCATC GTCGTGCTCC TCTCAGGAGA AGCTGCACCA GTTGCCTTTC
    CAGCCCACGG CCGATGAGCT GCACTTCTTG ACGAAGCATT TCAGCACAGA CAGTGTGCCT
    GATGAGGAGG GGCGGCAGTC CCCCGCCCTG CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA
    CGGTCACCAG TTTCCTTTGA CAGTGAAATA ATAATGATGA ATCATGTATA CAAGGAAAGA
    TTCCCCAAGG CCACTGCACA AATGGAAGAG CGTCTGGCCG AGTTCATCTC CTCCAATACT
    CCAGACAACG TGTTGCCCTT GGCTGATGGA ACCTTGAGCT TTATTCATCA TCAGGTGATA
    GAGATGGCCC GAGACTGCCT GGACAAATCC CGGAGTGGCC TCATTACCTC ACACTACTTC
    TACGAACTTC AAGAGAATTT AGAGAAGCTT CTGCAAGATG CTCATGAGCG CTCAGAGAGT
    TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG AAAAAGCTGA TGATTGTCAT TGCCCGCCCA
    GCTCGTCTCC TGGAATGCCT GGAGTTTGAC CCTGAAGAGT TCTACCACCT GTTAGAAGCA
    GCTGAGGGCC ACGCCAAAGA GGGACAAGGG ATTAAATGTG ACATTCCCCG CTACATCGTT
    AGCCAACTGG GCCTCACACG GGATCCCCTA GAGGAAATGG CCCAGTTGAG CAGCTATGAC
    AGTCCTGATA CTCCGGAGAC AGATGATTCA GTCGAGGGCC GTGGGGCATC TCTGACATCG
    AAAAAGACAC CCTCTGAGGA GGACTTCGAA ACCATTAAGC TCATCAGCAA TGGCGCCTAT
    GGGGCCGTGT TCCTGGTGCG GCACAAGTCC ACCCGGCAGC GCTTTGCCAT GAAGAAGATC
    AACAAGCAGA ACCTGATCCT GCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGGGACATC
    CTGACTTTTG CTGAGAACCC CTTTGTGGTC AGCATGTTCT GCTCCTTTGA AACCAAGCGC
    CACCTGTGCA TGGTGATGGA GTATGTGGAA GGAGGAGACT GTGCCACGCT GCTGAAGAAC
    ATCGGGGCCC TGCCTGTGGA CATGGTGCGC CTGTACTTTG CAGAAACTGT GCTAGCCCTG
    GAGTACTTAC ACAATTACGG CATCGTGCAC CGTGACCTCA AGCCTGACAA CCTGCTGATT
    ACATCCATGG GTCACATCAA GCTCACTGAT TTTGGCCTTT CCAAAATTGG CCTTATGAGT
    CTGACCACAA ACTTGTACGA GGGCCACATT GAAAAGGATG CACGGGAGTT CCTGGACAAG
    CAGGTATGCG GGACCCCGGA GTACATTGCA CCTGAGGTGA TCCTGCGGCA GGGCTACGGG
    AAGCCAGTGG ACTGGTGGGC TATGGGCATC ATCCTGTACG AGTTCCTGGT GGGCTGCGTC
    CCTTTCTTTG GAGACACTCC GGAGGAGCTC TTTGGGCAAG TGATCAGTGA TGAGATTGTG
    TGGCCTGAGG GGGACGATGC ACTGCCGCCG GACGCCCAAG ATCTCACCTC AAAACTGCTC
    CACCAGAACC CATTGGAGAG ACTGGGCACA GGCAGTGCCT ATGAGGTGAA GCAGCACCCA
    TTCTTCACTG GTCTGGACTG GACGGGACTT CTTCGCCAGA AGGCTGAATT TATTCCTCAG
    CTGGAGTCAG AGGATGATAC CAGCTACTTT GACACCCGCT CAGAGCGATA CCACCACGTG
    GACTCGGAGG ATGAGGAGGA AGTGAGTGAG GATGGCTGCC TTGAGATTCG CCAGTTCTCG
    TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC AGCAGCATGG AGCGGCTCTC GCTGCTCGAG
    GAGCGCCGGA CGCCACCCCC CACCAAGCGC AGCCTGAGTG AGGAAAAGGA GGACCGCACG
    GATGGCCTGG CGGGGCTGAG GGGCCGAGAG CGAAGCTGGG TGATCGGCTC CCCTGAGATA
    TTACGGAAGC GGCTGTCGGT GTCCGAGTCG TCCCACACGG AAAGCGATTC CAGCCCTCCA
    CTCACCGTGC GGCGCCACTG CTCGGGCCTC CTGGACGTGC CTCGCTTCCC TGAGAGCACT
    GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG CAGCAGGAGG GTGCATGGCT TCTGACCCCG
    TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG GAACGGCCGG CCGAGCGGCG GCTGAAGCTG
    AATGAGGAAC CTGCTGGCCA GAGCAGCGCC CCCAGCCCAG CCATGGAGAC CCGAGGAGGC
    CGCGGAACCC CACAGCTGGC AGAAGGAGCC ACAGCCAGGG CCATCAGCGA CCTGGCGGTG
    CGCAGGGCCC GCCACCGGCT GCTCTCTGGG GACTCCATAG AAAAGCGCAC CGCTCGCCCC
    ATCAACAAAG TGATCAAGTC CGCCTCAGCC ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG
    CACCACTCCT GCTCTCCACT GGCCAGCCCC ATGTCCCCAC ATTCCCAGTC ATCCAACCCA
    TCATCCAGGG ACTCTTCTCC AAGCAGGGAC TTCCTGCCAG CCCTCAGCAG CTCACGGCCC
    CCCATTGTCA TCCACCGAGC CGGCAAGAAG TATGGCTTCA CCCTACGAGC CATCCGTGTC
    TACATGGGTG ACTCAGATGT CTACACCGTG CACCACATGG TGTGGCACGT GGAGGACGGA
    GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG GGGGACCTCA TCACGCACGT CAACGGGGAG
    CCTGTGCACG GGCTGGTGCA CACGGAGGTG GTGGAGCTGA TCCTGAAGAG TGGAAACAAG
    GTGTCTATTT CAACAACTCC CCTGGAGAAC ACCTCCATTA AGGTGGGGCC CGCTCGGAAG
    GGCAGCTACA AGGCCAAGAT GGCCCGAAGG AGCAAGCGGA GCCGGGGCAA GGATGGACAA
    GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG AAGATCACCA AGCAGGCATC CCTGCTCCAC
    ACCAGCCGCA GCCTTTCTTC CCTTAACCGC TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC
    TCGCCCACAC ACAGCCACAG CCTTTCTCCC CGCTCTCCCA CGCAGGGCTA CCGGGTGACC
    CCTGAAGCCG TGCACTCAGT GGGGGGGAAT TCATCACAGA GCAGCTCCCC CAGCTCCAGC
    GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC ACACGGCCGA GCTCCCTCCA CGGTCTGGCG
    CCCAAGCTCC AACGCCAGTA CCGCTCTCCA CGGCGCAAGT CAGCAGGCAG CATCCCGCTG
    TCACCACTGG CCCACACCCC TTCACCACCA CCAGCGGCTT CGCCTCAGCG TTCCCCATCA
    CCTCTGTCTG GCCACGGGGC CCAGGCCTTC CCTACCAAGC TCCACTTGTC GCCTCCCCTA
    GGCCGGCAAC TCTCACGGCC CAAGAGCGCG GAGCCACCCC GCTCACCACT ACTTAAGAGG
    GTGCAGTCAG CTGAAAAACT GGCAGCTGCA CTGGCTGCTT CTGAGAAGAA GCTCGCCACT
    TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC CCCGAGCTAA AGAAGGAATT GCCGCCCAGG
    GAAGTCAGCT CTCTGGAGGT AGTTGGGACC AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG
    CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC TCACGGGCCT TAGGGACCCT CCGCCAGGAC
    AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG CAGGAAGCCA TCCGGGAGGT TGACTCTTCA
    GAAGATGATA CTGATGAGGG GCCTGAGAAC AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA
    CCCCACCGAG AAGGGGGCCA GGATCCACCC CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG
    GATGCCTTCT TGCCCAGGGA TCCCAGGGGA CAGGGCCCAG TGGTCACAGG TCTACTGACA
    GGGGTCACAC TGGGATCTCC CAGAATGGAA GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA
    ATCCTACAGG CTTTTGAAGA GGGTTCCAGC TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG
    GCTGGCCCCA CAGACCCCAT CCCCGCTGAA GGCTGCTGGA AGGCCCAGCA CCTCCACACC
    CAGGCACTGA CAGCACTTTG TCCGAGCTCA TCAGGACGCA TCCCTGCCAG TTGCTCCACT
    GCCTCCACCC CTGGAGAGCT GGGCCCATGG TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC
    AGGGCATACC CAACCGGAAA GGCAGCAATG GAAGATGGGC CAGCCAGCTC CCAAGATTTG
    GAAACTACAA CTCCAGGCCA CCCTGCAAAC CTGTCCCCCA GACAGGAGGG GAAGTCATGG
    CCACCCAGTG CCCCTGGGCT GGCCCATCCA CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA
    TGGGAGTCTG AGTGTGCACA GAGAGAGAAA GAGGAGCCAG CGCTGGGCAT CACCAAAGTG
    CCTGATGCCT CAGGTGACAG GAGGCAGGAC GTTCCATGCA AGAGCTGCCC CTTCACCCCA
    GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC CAAGACCCAG GGGGCCCTCA GGAGCATCAG
    GACTTGGCAT TGTTACCTGA TGAGCTTTTA AAGCAAACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005204943.1
    CDS242..5203
    Translation

    Target ORF information:

    RefSeq Version XM_005204886.4
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204886.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA CTGTCGGACA 
    AGTAACCGCA AGAGTTTGAT TGTGACCTCT AGTACGTCAC CTACACTGCC ACGGCCACAC
    TCACCACTCC ATGGCCACAC AGGTAACAGT CCTTTGGATA GTCCCCGGAA TTTCTCTCCA
    AATGCACCTG CTCACTTTTC CTTTGTTCCT GCCCGTAGCC ATGGCCACAG AGCAGACAGG
    ACTGATGGAC GGCGCTGGTC TTTGGCCTCA TTGCCTTCTT CAGGCTATGG AACCAACACC
    CCTAGTTCTA CCGTGTCATC GTCGTGCTCC TCTCAGGAGA AGCTGCACCA GTTGCCTTTC
    CAGCCCACGG CCGATGAGCT GCACTTCTTG ACGAAGCATT TCAGCACAGA CAGTGTGCCT
    GATGAGGAGG GGCGGCAGTC CCCCGCCCTG CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA
    CGGTCACCAG TTTCCTTTGA CAGTGAAATA ATAATGATGA ATCATGTATA CAAGGAAAGA
    TTCCCCAAGG CCACTGCACA AATGGAAGAG CGTCTGGCCG AGTTCATCTC CTCCAATACT
    CCAGACAACG TGTTGCCCTT GGCTGATGGA ACCTTGAGCT TTATTCATCA TCAGGTGATA
    GAGATGGCCC GAGACTGCCT GGACAAATCC CGGAGTGGCC TCATTACCTC ACACTACTTC
    TACGAACTTC AAGAGAATTT AGAGAAGCTT CTGCAAGATG CTCATGAGCG CTCAGAGAGT
    TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG AAAAAGCTGA TGATTGTCAT TGCCCGCCCA
    GCTCGTCTCC TGGAATGCCT GGAGTTTGAC CCTGAAGAGT TCTACCACCT GTTAGAAGCA
    GCTGAGGGCC ACGCCAAAGA GGGACAAGGG ATTAAATGTG ACATTCCCCG CTACATCGTT
    AGCCAACTGG GCCTCACACG GGATCCCCTA GAGGAAATGG CCCAGTTGAG CAGCTATGAC
    AGTCCTGATA CTCCGGAGAC AGATGATTCA GTCGAGGGCC GTGGGGCATC TCTGACATCG
    AAAAAGACAC CCTCTGAGGA GGACTTCGAA ACCATTAAGC TCATCAGCAA TGGCGCCTAT
    GGGGCCGTGT TCCTGGTGCG GCACAAGTCC ACCCGGCAGC GCTTTGCCAT GAAGAAGATC
    AACAAGCAGA ACCTGATCCT GCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGGGACATC
    CTGACTTTTG CTGAGAACCC CTTTGTGGTC AGCATGTTCT GCTCCTTTGA AACCAAGCGC
    CACCTGTGCA TGGTGATGGA GTATGTGGAA GGAGGAGACT GTGCCACGCT GCTGAAGAAC
    ATCGGGGCCC TGCCTGTGGA CATGGTGCGC CTGTACTTTG CAGAAACTGT GCTAGCCCTG
    GAGTACTTAC ACAATTACGG CATCGTGCAC CGTGACCTCA AGCCTGACAA CCTGCTGATT
    ACATCCATGG GTCACATCAA GCTCACTGAT TTTGGCCTTT CCAAAATTGG CCTTATGAGT
    CTGACCACAA ACTTGTACGA GGGCCACATT GAAAAGGATG CACGGGAGTT CCTGGACAAG
    CAGGTATGCG GGACCCCGGA GTACATTGCA CCTGAGGTGA TCCTGCGGCA GGGCTACGGG
    AAGCCAGTGG ACTGGTGGGC TATGGGCATC ATCCTGTACG AGTTCCTGGT GGGCTGCGTC
    CCTTTCTTTG GAGACACTCC GGAGGAGCTC TTTGGGCAAG TGATCAGTGA TGAGATTGTG
    TGGCCTGAGG GGGACGATGC ACTGCCGCCG GACGCCCAAG ATCTCACCTC AAAACTGCTC
    CACCAGAACC CATTGGAGAG ACTGGGCACA GGCAGTGCCT ATGAGGTGAA GCAGCACCCA
    TTCTTCACTG GTCTGGACTG GACGGGACTT CTTCGCCAGA AGGCTGAATT TATTCCTCAG
    CTGGAGTCAG AGGATGATAC CAGCTACTTT GACACCCGCT CAGAGCGATA CCACCACGTG
    GACTCGGAGG ATGAGGAGGA AGTGAGTGAG GATGGCTGCC TTGAGATTCG CCAGTTCTCG
    TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC AGCAGCATGG AGCGGCTCTC GCTGCTCGAG
    GAGCGCCGGA CGCCACCCCC CACCAAGCGC AGCCTGAGTG AGGAAAAGGA GGACCGCACG
    GATGGCCTGG CGGGGCTGAG GGGCCGAGAG CGAAGCTGGG TGATCGGCTC CCCTGAGATA
    TTACGGAAGC GGCTGTCGGT GTCCGAGTCG TCCCACACGG AAAGCGATTC CAGCCCTCCA
    CTCACCGTGC GGCGCCACTG CTCGGGCCTC CTGGACGTGC CTCGCTTCCC TGAGAGCACT
    GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG CAGCAGGAGG GTGCATGGCT TCTGACCCCG
    TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG GAACGGCCGG CCGAGCGGCG GCTGAAGCTG
    AATGAGGAAC CTGCTGGCCA GAGCAGCGCC CCCAGCCCAG CCATGGAGAC CCGAGGAGGC
    CGCGGAACCC CACAGCTGGC AGAAGGAGCC ACAGCCAGGG CCATCAGCGA CCTGGCGGTG
    CGCAGGGCCC GCCACCGGCT GCTCTCTGGG GACTCCATAG AAAAGCGCAC CGCTCGCCCC
    ATCAACAAAG TGATCAAGTC CGCCTCAGCC ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG
    CACCACTCCT GCTCTCCACT GGCCAGCCCC ATGTCCCCAC ATTCCCAGTC ATCCAACCCA
    TCATCCAGGG ACTCTTCTCC AAGCAGGGAC TTCCTGCCAG CCCTCAGCAG CTCACGGCCC
    CCCATTGTCA TCCACCGAGC CGGCAAGAAG TATGGCTTCA CCCTACGAGC CATCCGTGTC
    TACATGGGTG ACTCAGATGT CTACACCGTG CACCACATGG TGTGGCACGT GGAGGACGGA
    GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG GGGGACCTCA TCACGCACGT CAACGGGGAG
    CCTGTGCACG GGCTGGTGCA CACGGAGGTG GTGGAGCTGA TCCTGAAGAG TGGAAACAAG
    GTGTCTATTT CAACAACTCC CCTGGAGAAC ACCTCCATTA AGGTGGGGCC CGCTCGGAAG
    GGCAGCTACA AGGCCAAGAT GGCCCGAAGG AGCAAGCGGA GCCGGGGCAA GGATGGACAA
    GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG AAGATCACCA AGCAGGCATC CCTGCTCCAC
    ACCAGCCGCA GCCTTTCTTC CCTTAACCGC TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC
    TCGCCCACAC ACAGCCACAG CCTTTCTCCC CGCTCTCCCA CGCAGGGCTA CCGGGTGACC
    CCTGAAGCCG TGCACTCAGT GGGGGGGAAT TCATCACAGA GCAGCTCCCC CAGCTCCAGC
    GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC ACACGGCCGA GCTCCCTCCA CGGTCTGGCG
    CCCAAGCTCC AACGCCAGTA CCGCTCTCCA CGGCGCAAGT CAGCAGGCAG CATCCCGCTG
    TCACCACTGG CCCACACCCC TTCACCACCA CCAGCGGCTT CGCCTCAGCG TTCCCCATCA
    CCTCTGTCTG GCCACGGGGC CCAGGCCTTC CCTACCAAGC TCCACTTGTC GCCTCCCCTA
    GGCCGGCAAC TCTCACGGCC CAAGAGCGCG GAGCCACCCC GCTCACCACT ACTTAAGAGG
    GTGCAGTCAG CTGAAAAACT GGCAGCTGCA CTGGCTGCTT CTGAGAAGAA GCTCGCCACT
    TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC CCCGAGCTAA AGAAGGAATT GCCGCCCAGG
    GAAGTCAGCT CTCTGGAGGT AGTTGGGACC AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG
    CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC TCACGGGCCT TAGGGACCCT CCGCCAGGAC
    AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG CAGGAAGCCA TCCGGGAGGT TGACTCTTCA
    GAAGATGATA CTGATGAGGG GCCTGAGAAC AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA
    CCCCACCGAG AAGGGGGCCA GGATCCACCC CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG
    GATGCCTTCT TGCCCAGGGA TCCCAGGGGA CAGGGCCCAG TGGTCACAGG TCTACTGACA
    GGGGTCACAC TGGGATCTCC CAGAATGGAA GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA
    ATCCTACAGG CTTTTGAAGA GGGTTCCAGC TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG
    GCTGGCCCCA CAGACCCCAT CCCCGCTGAA GGCTGCTGGA AGGCCCAGCA CCTCCACACC
    CAGGCACTGA CAGCACTTTG TCCGAGCTCA TCAGGACGCA TCCCTGCCAG TTGCTCCACT
    GCCTCCACCC CTGGAGAGCT GGGCCCATGG TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC
    AGGGCATACC CAACCGGAAA GGCAGCAATG GAAGATGGGC CAGCCAGCTC CCAAGATTTG
    GAAACTACAA CTCCAGGCCA CCCTGCAAAC CTGTCCCCCA GACAGGAGGG GAAGTCATGG
    CCACCCAGTG CCCCTGGGCT GGCCCATCCA CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA
    TGGGAGTCTG AGTGTGCACA GAGAGAGAAA GAGGAGCCAG CGCTGGGCAT CACCAAAGTG
    CCTGATGCCT CAGGTGACAG GAGGCAGGAC GTTCCATGCA AGAGCTGCCC CTTCACCCCA
    GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC CAAGACCCAG GGGGCCCTCA GGAGCATCAG
    GACTTGGCAT TGTTACCTGA TGAGCTTTTA AAGCAAACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18165
    Clone ID Related Accession (Same CDS sequence) XM_005204886.3 , XM_005204886.4 , XM_015462790.1
    Accession Version XM_015462790.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA CTGTCGGACA 
    AGTAACCGCA AGAGTTTGAT TGTGACCTCT AGTACGTCAC CTACACTGCC ACGGCCACAC
    TCACCACTCC ATGGCCACAC AGGTAACAGT CCTTTGGATA GTCCCCGGAA TTTCTCTCCA
    AATGCACCTG CTCACTTTTC CTTTGTTCCT GCCCGTAGCC ATGGCCACAG AGCAGACAGG
    ACTGATGGAC GGCGCTGGTC TTTGGCCTCA TTGCCTTCTT CAGGCTATGG AACCAACACC
    CCTAGTTCTA CCGTGTCATC GTCGTGCTCC TCTCAGGAGA AGCTGCACCA GTTGCCTTTC
    CAGCCCACGG CCGATGAGCT GCACTTCTTG ACGAAGCATT TCAGCACAGA CAGTGTGCCT
    GATGAGGAGG GGCGGCAGTC CCCCGCCCTG CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA
    CGGTCACCAG TTTCCTTTGA CAGTGAAATA ATAATGATGA ATCATGTATA CAAGGAAAGA
    TTCCCCAAGG CCACTGCACA AATGGAAGAG CGTCTGGCCG AGTTCATCTC CTCCAATACT
    CCAGACAACG TGTTGCCCTT GGCTGATGGA ACCTTGAGCT TTATTCATCA TCAGGTGATA
    GAGATGGCCC GAGACTGCCT GGACAAATCC CGGAGTGGCC TCATTACCTC ACACTACTTC
    TACGAACTTC AAGAGAATTT AGAGAAGCTT CTGCAAGATG CTCATGAGCG CTCAGAGAGT
    TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG AAAAAGCTGA TGATTGTCAT TGCCCGCCCA
    GCTCGTCTCC TGGAATGCCT GGAGTTTGAC CCTGAAGAGT TCTACCACCT GTTAGAAGCA
    GCTGAGGGCC ACGCCAAAGA GGGACAAGGG ATTAAATGTG ACATTCCCCG CTACATCGTT
    AGCCAACTGG GCCTCACACG GGATCCCCTA GAGGAAATGG CCCAGTTGAG CAGCTATGAC
    AGTCCTGATA CTCCGGAGAC AGATGATTCA GTCGAGGGCC GTGGGGCATC TCTGACATCG
    AAAAAGACAC CCTCTGAGGA GGACTTCGAA ACCATTAAGC TCATCAGCAA TGGCGCCTAT
    GGGGCCGTGT TCCTGGTGCG GCACAAGTCC ACCCGGCAGC GCTTTGCCAT GAAGAAGATC
    AACAAGCAGA ACCTGATCCT GCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGGGACATC
    CTGACTTTTG CTGAGAACCC CTTTGTGGTC AGCATGTTCT GCTCCTTTGA AACCAAGCGC
    CACCTGTGCA TGGTGATGGA GTATGTGGAA GGAGGAGACT GTGCCACGCT GCTGAAGAAC
    ATCGGGGCCC TGCCTGTGGA CATGGTGCGC CTGTACTTTG CAGAAACTGT GCTAGCCCTG
    GAGTACTTAC ACAATTACGG CATCGTGCAC CGTGACCTCA AGCCTGACAA CCTGCTGATT
    ACATCCATGG GTCACATCAA GCTCACTGAT TTTGGCCTTT CCAAAATTGG CCTTATGAGT
    CTGACCACAA ACTTGTACGA GGGCCACATT GAAAAGGATG CACGGGAGTT CCTGGACAAG
    CAGGTATGCG GGACCCCGGA GTACATTGCA CCTGAGGTGA TCCTGCGGCA GGGCTACGGG
    AAGCCAGTGG ACTGGTGGGC TATGGGCATC ATCCTGTACG AGTTCCTGGT GGGCTGCGTC
    CCTTTCTTTG GAGACACTCC GGAGGAGCTC TTTGGGCAAG TGATCAGTGA TGAGATTGTG
    TGGCCTGAGG GGGACGATGC ACTGCCGCCG GACGCCCAAG ATCTCACCTC AAAACTGCTC
    CACCAGAACC CATTGGAGAG ACTGGGCACA GGCAGTGCCT ATGAGGTGAA GCAGCACCCA
    TTCTTCACTG GTCTGGACTG GACGGGACTT CTTCGCCAGA AGGCTGAATT TATTCCTCAG
    CTGGAGTCAG AGGATGATAC CAGCTACTTT GACACCCGCT CAGAGCGATA CCACCACGTG
    GACTCGGAGG ATGAGGAGGA AGTGAGTGAG GATGGCTGCC TTGAGATTCG CCAGTTCTCG
    TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC AGCAGCATGG AGCGGCTCTC GCTGCTCGAG
    GAGCGCCGGA CGCCACCCCC CACCAAGCGC AGCCTGAGTG AGGAAAAGGA GGACCGCACG
    GATGGCCTGG CGGGGCTGAG GGGCCGAGAG CGAAGCTGGG TGATCGGCTC CCCTGAGATA
    TTACGGAAGC GGCTGTCGGT GTCCGAGTCG TCCCACACGG AAAGCGATTC CAGCCCTCCA
    CTCACCGTGC GGCGCCACTG CTCGGGCCTC CTGGACGTGC CTCGCTTCCC TGAGAGCACT
    GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG CAGCAGGAGG GTGCATGGCT TCTGACCCCG
    TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG GAACGGCCGG CCGAGCGGCG GCTGAAGCTG
    AATGAGGAAC CTGCTGGCCA GAGCAGCGCC CCCAGCCCAG CCATGGAGAC CCGAGGAGGC
    CGCGGAACCC CACAGCTGGC AGAAGGAGCC ACAGCCAGGG CCATCAGCGA CCTGGCGGTG
    CGCAGGGCCC GCCACCGGCT GCTCTCTGGG GACTCCATAG AAAAGCGCAC CGCTCGCCCC
    ATCAACAAAG TGATCAAGTC CGCCTCAGCC ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG
    CACCACTCCT GCTCTCCACT GGCCAGCCCC ATGTCCCCAC ATTCCCAGTC ATCCAACCCA
    TCATCCAGGG ACTCTTCTCC AAGCAGGGAC TTCCTGCCAG CCCTCAGCAG CTCACGGCCC
    CCCATTGTCA TCCACCGAGC CGGCAAGAAG TATGGCTTCA CCCTACGAGC CATCCGTGTC
    TACATGGGTG ACTCAGATGT CTACACCGTG CACCACATGG TGTGGCACGT GGAGGACGGA
    GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG GGGGACCTCA TCACGCACGT CAACGGGGAG
    CCTGTGCACG GGCTGGTGCA CACGGAGGTG GTGGAGCTGA TCCTGAAGAG TGGAAACAAG
    GTGTCTATTT CAACAACTCC CCTGGAGAAC ACCTCCATTA AGGTGGGGCC CGCTCGGAAG
    GGCAGCTACA AGGCCAAGAT GGCCCGAAGG AGCAAGCGGA GCCGGGGCAA GGATGGACAA
    GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG AAGATCACCA AGCAGGCATC CCTGCTCCAC
    ACCAGCCGCA GCCTTTCTTC CCTTAACCGC TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC
    TCGCCCACAC ACAGCCACAG CCTTTCTCCC CGCTCTCCCA CGCAGGGCTA CCGGGTGACC
    CCTGAAGCCG TGCACTCAGT GGGGGGGAAT TCATCACAGA GCAGCTCCCC CAGCTCCAGC
    GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC ACACGGCCGA GCTCCCTCCA CGGTCTGGCG
    CCCAAGCTCC AACGCCAGTA CCGCTCTCCA CGGCGCAAGT CAGCAGGCAG CATCCCGCTG
    TCACCACTGG CCCACACCCC TTCACCACCA CCAGCGGCTT CGCCTCAGCG TTCCCCATCA
    CCTCTGTCTG GCCACGGGGC CCAGGCCTTC CCTACCAAGC TCCACTTGTC GCCTCCCCTA
    GGCCGGCAAC TCTCACGGCC CAAGAGCGCG GAGCCACCCC GCTCACCACT ACTTAAGAGG
    GTGCAGTCAG CTGAAAAACT GGCAGCTGCA CTGGCTGCTT CTGAGAAGAA GCTCGCCACT
    TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC CCCGAGCTAA AGAAGGAATT GCCGCCCAGG
    GAAGTCAGCT CTCTGGAGGT AGTTGGGACC AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG
    CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC TCACGGGCCT TAGGGACCCT CCGCCAGGAC
    AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG CAGGAAGCCA TCCGGGAGGT TGACTCTTCA
    GAAGATGATA CTGATGAGGG GCCTGAGAAC AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA
    CCCCACCGAG AAGGGGGCCA GGATCCACCC CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG
    GATGCCTTCT TGCCCAGGGA TCCCAGGGGA CAGGGCCCAG TGGTCACAGG TCTACTGACA
    GGGGTCACAC TGGGATCTCC CAGAATGGAA GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA
    ATCCTACAGG CTTTTGAAGA GGGTTCCAGC TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG
    GCTGGCCCCA CAGACCCCAT CCCCGCTGAA GGCTGCTGGA AGGCCCAGCA CCTCCACACC
    CAGGCACTGA CAGCACTTTG TCCGAGCTCA TCAGGACGCA TCCCTGCCAG TTGCTCCACT
    GCCTCCACCC CTGGAGAGCT GGGCCCATGG TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC
    AGGGCATACC CAACCGGAAA GGCAGCAATG GAAGATGGGC CAGCCAGCTC CCAAGATTTG
    GAAACTACAA CTCCAGGCCA CCCTGCAAAC CTGTCCCCCA GACAGGAGGG GAAGTCATGG
    CCACCCAGTG CCCCTGGGCT GGCCCATCCA CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA
    TGGGAGTCTG AGTGTGCACA GAGAGAGAAA GAGGAGCCAG CGCTGGGCAT CACCAAAGTG
    CCTGATGCCT CAGGTGACAG GAGGCAGGAC GTTCCATGCA AGAGCTGCCC CTTCACCCCA
    GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC CAAGACCCAG GGGGCCCTCA GGAGCATCAG
    GACTTGGCAT TGTTACCTGA TGAGCTTTTA AAGCAAACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318276.1
    CDS263..5224
    Translation

    Target ORF information:

    RefSeq Version XM_015462790.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462790.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGAAAC GGAGGAGAGA GAGACTGGGA GCACCATGCC TGCGGATCCA CTGTCGGACA 
    AGTAACCGCA AGAGTTTGAT TGTGACCTCT AGTACGTCAC CTACACTGCC ACGGCCACAC
    TCACCACTCC ATGGCCACAC AGGTAACAGT CCTTTGGATA GTCCCCGGAA TTTCTCTCCA
    AATGCACCTG CTCACTTTTC CTTTGTTCCT GCCCGTAGCC ATGGCCACAG AGCAGACAGG
    ACTGATGGAC GGCGCTGGTC TTTGGCCTCA TTGCCTTCTT CAGGCTATGG AACCAACACC
    CCTAGTTCTA CCGTGTCATC GTCGTGCTCC TCTCAGGAGA AGCTGCACCA GTTGCCTTTC
    CAGCCCACGG CCGATGAGCT GCACTTCTTG ACGAAGCATT TCAGCACAGA CAGTGTGCCT
    GATGAGGAGG GGCGGCAGTC CCCCGCCCTG CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA
    CGGTCACCAG TTTCCTTTGA CAGTGAAATA ATAATGATGA ATCATGTATA CAAGGAAAGA
    TTCCCCAAGG CCACTGCACA AATGGAAGAG CGTCTGGCCG AGTTCATCTC CTCCAATACT
    CCAGACAACG TGTTGCCCTT GGCTGATGGA ACCTTGAGCT TTATTCATCA TCAGGTGATA
    GAGATGGCCC GAGACTGCCT GGACAAATCC CGGAGTGGCC TCATTACCTC ACACTACTTC
    TACGAACTTC AAGAGAATTT AGAGAAGCTT CTGCAAGATG CTCATGAGCG CTCAGAGAGT
    TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG AAAAAGCTGA TGATTGTCAT TGCCCGCCCA
    GCTCGTCTCC TGGAATGCCT GGAGTTTGAC CCTGAAGAGT TCTACCACCT GTTAGAAGCA
    GCTGAGGGCC ACGCCAAAGA GGGACAAGGG ATTAAATGTG ACATTCCCCG CTACATCGTT
    AGCCAACTGG GCCTCACACG GGATCCCCTA GAGGAAATGG CCCAGTTGAG CAGCTATGAC
    AGTCCTGATA CTCCGGAGAC AGATGATTCA GTCGAGGGCC GTGGGGCATC TCTGACATCG
    AAAAAGACAC CCTCTGAGGA GGACTTCGAA ACCATTAAGC TCATCAGCAA TGGCGCCTAT
    GGGGCCGTGT TCCTGGTGCG GCACAAGTCC ACCCGGCAGC GCTTTGCCAT GAAGAAGATC
    AACAAGCAGA ACCTGATCCT GCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGGGACATC
    CTGACTTTTG CTGAGAACCC CTTTGTGGTC AGCATGTTCT GCTCCTTTGA AACCAAGCGC
    CACCTGTGCA TGGTGATGGA GTATGTGGAA GGAGGAGACT GTGCCACGCT GCTGAAGAAC
    ATCGGGGCCC TGCCTGTGGA CATGGTGCGC CTGTACTTTG CAGAAACTGT GCTAGCCCTG
    GAGTACTTAC ACAATTACGG CATCGTGCAC CGTGACCTCA AGCCTGACAA CCTGCTGATT
    ACATCCATGG GTCACATCAA GCTCACTGAT TTTGGCCTTT CCAAAATTGG CCTTATGAGT
    CTGACCACAA ACTTGTACGA GGGCCACATT GAAAAGGATG CACGGGAGTT CCTGGACAAG
    CAGGTATGCG GGACCCCGGA GTACATTGCA CCTGAGGTGA TCCTGCGGCA GGGCTACGGG
    AAGCCAGTGG ACTGGTGGGC TATGGGCATC ATCCTGTACG AGTTCCTGGT GGGCTGCGTC
    CCTTTCTTTG GAGACACTCC GGAGGAGCTC TTTGGGCAAG TGATCAGTGA TGAGATTGTG
    TGGCCTGAGG GGGACGATGC ACTGCCGCCG GACGCCCAAG ATCTCACCTC AAAACTGCTC
    CACCAGAACC CATTGGAGAG ACTGGGCACA GGCAGTGCCT ATGAGGTGAA GCAGCACCCA
    TTCTTCACTG GTCTGGACTG GACGGGACTT CTTCGCCAGA AGGCTGAATT TATTCCTCAG
    CTGGAGTCAG AGGATGATAC CAGCTACTTT GACACCCGCT CAGAGCGATA CCACCACGTG
    GACTCGGAGG ATGAGGAGGA AGTGAGTGAG GATGGCTGCC TTGAGATTCG CCAGTTCTCG
    TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC AGCAGCATGG AGCGGCTCTC GCTGCTCGAG
    GAGCGCCGGA CGCCACCCCC CACCAAGCGC AGCCTGAGTG AGGAAAAGGA GGACCGCACG
    GATGGCCTGG CGGGGCTGAG GGGCCGAGAG CGAAGCTGGG TGATCGGCTC CCCTGAGATA
    TTACGGAAGC GGCTGTCGGT GTCCGAGTCG TCCCACACGG AAAGCGATTC CAGCCCTCCA
    CTCACCGTGC GGCGCCACTG CTCGGGCCTC CTGGACGTGC CTCGCTTCCC TGAGAGCACT
    GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG CAGCAGGAGG GTGCATGGCT TCTGACCCCG
    TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG GAACGGCCGG CCGAGCGGCG GCTGAAGCTG
    AATGAGGAAC CTGCTGGCCA GAGCAGCGCC CCCAGCCCAG CCATGGAGAC CCGAGGAGGC
    CGCGGAACCC CACAGCTGGC AGAAGGAGCC ACAGCCAGGG CCATCAGCGA CCTGGCGGTG
    CGCAGGGCCC GCCACCGGCT GCTCTCTGGG GACTCCATAG AAAAGCGCAC CGCTCGCCCC
    ATCAACAAAG TGATCAAGTC CGCCTCAGCC ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG
    CACCACTCCT GCTCTCCACT GGCCAGCCCC ATGTCCCCAC ATTCCCAGTC ATCCAACCCA
    TCATCCAGGG ACTCTTCTCC AAGCAGGGAC TTCCTGCCAG CCCTCAGCAG CTCACGGCCC
    CCCATTGTCA TCCACCGAGC CGGCAAGAAG TATGGCTTCA CCCTACGAGC CATCCGTGTC
    TACATGGGTG ACTCAGATGT CTACACCGTG CACCACATGG TGTGGCACGT GGAGGACGGA
    GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG GGGGACCTCA TCACGCACGT CAACGGGGAG
    CCTGTGCACG GGCTGGTGCA CACGGAGGTG GTGGAGCTGA TCCTGAAGAG TGGAAACAAG
    GTGTCTATTT CAACAACTCC CCTGGAGAAC ACCTCCATTA AGGTGGGGCC CGCTCGGAAG
    GGCAGCTACA AGGCCAAGAT GGCCCGAAGG AGCAAGCGGA GCCGGGGCAA GGATGGACAA
    GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG AAGATCACCA AGCAGGCATC CCTGCTCCAC
    ACCAGCCGCA GCCTTTCTTC CCTTAACCGC TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC
    TCGCCCACAC ACAGCCACAG CCTTTCTCCC CGCTCTCCCA CGCAGGGCTA CCGGGTGACC
    CCTGAAGCCG TGCACTCAGT GGGGGGGAAT TCATCACAGA GCAGCTCCCC CAGCTCCAGC
    GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC ACACGGCCGA GCTCCCTCCA CGGTCTGGCG
    CCCAAGCTCC AACGCCAGTA CCGCTCTCCA CGGCGCAAGT CAGCAGGCAG CATCCCGCTG
    TCACCACTGG CCCACACCCC TTCACCACCA CCAGCGGCTT CGCCTCAGCG TTCCCCATCA
    CCTCTGTCTG GCCACGGGGC CCAGGCCTTC CCTACCAAGC TCCACTTGTC GCCTCCCCTA
    GGCCGGCAAC TCTCACGGCC CAAGAGCGCG GAGCCACCCC GCTCACCACT ACTTAAGAGG
    GTGCAGTCAG CTGAAAAACT GGCAGCTGCA CTGGCTGCTT CTGAGAAGAA GCTCGCCACT
    TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC CCCGAGCTAA AGAAGGAATT GCCGCCCAGG
    GAAGTCAGCT CTCTGGAGGT AGTTGGGACC AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG
    CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC TCACGGGCCT TAGGGACCCT CCGCCAGGAC
    AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG CAGGAAGCCA TCCGGGAGGT TGACTCTTCA
    GAAGATGATA CTGATGAGGG GCCTGAGAAC AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA
    CCCCACCGAG AAGGGGGCCA GGATCCACCC CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG
    GATGCCTTCT TGCCCAGGGA TCCCAGGGGA CAGGGCCCAG TGGTCACAGG TCTACTGACA
    GGGGTCACAC TGGGATCTCC CAGAATGGAA GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA
    ATCCTACAGG CTTTTGAAGA GGGTTCCAGC TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG
    GCTGGCCCCA CAGACCCCAT CCCCGCTGAA GGCTGCTGGA AGGCCCAGCA CCTCCACACC
    CAGGCACTGA CAGCACTTTG TCCGAGCTCA TCAGGACGCA TCCCTGCCAG TTGCTCCACT
    GCCTCCACCC CTGGAGAGCT GGGCCCATGG TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC
    AGGGCATACC CAACCGGAAA GGCAGCAATG GAAGATGGGC CAGCCAGCTC CCAAGATTTG
    GAAACTACAA CTCCAGGCCA CCCTGCAAAC CTGTCCCCCA GACAGGAGGG GAAGTCATGG
    CCACCCAGTG CCCCTGGGCT GGCCCATCCA CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA
    TGGGAGTCTG AGTGTGCACA GAGAGAGAAA GAGGAGCCAG CGCTGGGCAT CACCAAAGTG
    CCTGATGCCT CAGGTGACAG GAGGCAGGAC GTTCCATGCA AGAGCTGCCC CTTCACCCCA
    GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC CAAGACCCAG GGGGCCCTCA GGAGCATCAG
    GACTTGGCAT TGTTACCTGA TGAGCTTTTA AAGCAAACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53217
    Clone ID Related Accession (Same CDS sequence) XM_003582019.4 , XM_010803741.2 , XM_010803741.3
    Accession Version XM_003582019.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582019.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGATGATT CCAGTATTCT ACAAAGGAGA GGGTTACAGA AGGAGCTGAG CCTTCCAAGA 
    AGAGGCAGCT TCTGTCGGAC AAGTAACCGC AAGAGTTTGA TTGTGACCTC TAGTACGTCA
    CCTACACTGC CACGGCCACA CTCACCACTC CATGGCCACA CAGGTAACAG TCCTTTGGAT
    AGTCCCCGGA ATTTCTCTCC AAATGCACCT GCTCACTTTT CCTTTGTTCC TGCCCGTAGC
    CATGGCCACA GAGCAGACAG GACTGATGGA CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT
    TCAGGCTATG GAACCAACAC CCCTAGTTCT ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG
    AAGCTGCACC AGTTGCCTTT CCAGCCCACG GCCGATGAGC TGCACTTCTT GACGAAGCAT
    TTCAGCACAG ACAGTGTGCC TGATGAGGAG GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC
    TCCCGCAGCC TCAGTCCTGG ACGGTCACCA GTTTCCTTTG ACAGTGAAAT AATAATGATG
    AATCATGTAT ACAAGGAAAG ATTCCCCAAG GCCACTGCAC AAATGGAAGA GCGTCTGGCC
    GAGTTCATCT CCTCCAATAC TCCAGACAAC GTGTTGCCCT TGGCTGATGG AACCTTGAGC
    TTTATTCATC ATCAGGTGAT AGAGATGGCC CGAGACTGCC TGGACAAATC CCGGAGTGGC
    CTCATTACCT CACACTACTT CTACGAACTT CAAGAGAATT TAGAGAAGCT TCTGCAAGAT
    GCTCATGAGC GCTCAGAGAG TTCAGAAGTG GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG
    ATGATTGTCA TTGCCCGCCC AGCTCGTCTC CTGGAATGCC TGGAGTTTGA CCCTGAAGAG
    TTCTACCACC TGTTAGAAGC AGCTGAGGGC CACGCCAAAG AGGGACAAGG GATTAAATGT
    GACATTCCCC GCTACATCGT TAGCCAACTG GGCCTCACAC GGGATCCCCT AGAGGAAATG
    GCCCAGTTGA GCAGCTATGA CAGTCCTGAT ACTCCGGAGA CAGATGATTC AGTCGAGGGC
    CGTGGGGCAT CTCTGACATC GAAAAAGACA CCCTCTGAGG AGGACTTCGA AACCATTAAG
    CTCATCAGCA ATGGCGCCTA TGGGGCCGTG TTCCTGGTGC GGCACAAGTC CACCCGGCAG
    CGCTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTGATCC TGCGGAACCA GATCCAGCAG
    GCCTTCGTGG AGCGGGACAT CCTGACTTTT GCTGAGAACC CCTTTGTGGT CAGCATGTTC
    TGCTCCTTTG AAACCAAGCG CCACCTGTGC ATGGTGATGG AGTATGTGGA AGGAGGAGAC
    TGTGCCACGC TGCTGAAGAA CATCGGGGCC CTGCCTGTGG ACATGGTGCG CCTGTACTTT
    GCAGAAACTG TGCTAGCCCT GGAGTACTTA CACAATTACG GCATCGTGCA CCGTGACCTC
    AAGCCTGACA ACCTGCTGAT TACATCCATG GGTCACATCA AGCTCACTGA TTTTGGCCTT
    TCCAAAATTG GCCTTATGAG TCTGACCACA AACTTGTACG AGGGCCACAT TGAAAAGGAT
    GCACGGGAGT TCCTGGACAA GCAGGTATGC GGGACCCCGG AGTACATTGC ACCTGAGGTG
    ATCCTGCGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CTATGGGCAT CATCCTGTAC
    GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT GGAGACACTC CGGAGGAGCT CTTTGGGCAA
    GTGATCAGTG ATGAGATTGT GTGGCCTGAG GGGGACGATG CACTGCCGCC GGACGCCCAA
    GATCTCACCT CAAAACTGCT CCACCAGAAC CCATTGGAGA GACTGGGCAC AGGCAGTGCC
    TATGAGGTGA AGCAGCACCC ATTCTTCACT GGTCTGGACT GGACGGGACT TCTTCGCCAG
    AAGGCTGAAT TTATTCCTCA GCTGGAGTCA GAGGATGATA CCAGCTACTT TGACACCCGC
    TCAGAGCGAT ACCACCACGT GGACTCGGAG GATGAGGAGG AAGTGAGTGA GGATGGCTGC
    CTTGAGATTC GCCAGTTCTC GTCCTGTTCT CCAAGGTTCA GCAAGGTGTA CAGCAGCATG
    GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG ACGCCACCCC CCACCAAGCG CAGCCTGAGT
    GAGGAAAAGG AGGACCGCAC GGATGGCCTG GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG
    GTGATCGGCT CCCCTGAGAT ATTACGGAAG CGGCTGTCGG TGTCCGAGTC GTCCCACACG
    GAAAGCGATT CCAGCCCTCC ACTCACCGTG CGGCGCCACT GCTCGGGCCT CCTGGACGTG
    CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG
    GGTGCATGGC TTCTGACCCC GTCTGGAGAG GGGGCGTCTG GGCCTGCCCT GGAACGGCCG
    GCCGAGCGGC GGCTGAAGCT GAATGAGGAA CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA
    GCCATGGAGA CCCGAGGAGG CCGCGGAACC CCACAGCTGG CAGAAGGAGC CACAGCCAGG
    GCCATCAGCG ACCTGGCGGT GCGCAGGGCC CGCCACCGGC TGCTCTCTGG GGACTCCATA
    GAAAAGCGCA CCGCTCGCCC CATCAACAAA GTGATCAAGT CCGCCTCAGC CACAGCCCTG
    TCCCTTCTCA TTCCTGCTGA GCACCACTCC TGCTCTCCAC TGGCCAGCCC CATGTCCCCA
    CATTCCCAGT CATCCAACCC ATCATCCAGG GACTCTTCTC CAAGCAGGGA CTTCCTGCCA
    GCCCTCAGCA GCTCACGGCC CCCCATTGTC ATCCACCGAG CCGGCAAGAA GTATGGCTTC
    ACCCTACGAG CCATCCGTGT CTACATGGGT GACTCAGATG TCTACACCGT GCACCACATG
    GTGTGGCACG TGGAGGACGG AGGTCCAGCC AGTGAGGCAG GGCTGCGTCA GGGGGACCTC
    ATCACGCACG TCAACGGGGA GCCTGTGCAC GGGCTGGTGC ACACGGAGGT GGTGGAGCTG
    ATCCTGAAGA GTGGAAACAA GGTGTCTATT TCAACAACTC CCCTGGAGAA CACCTCCATT
    AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG
    AGCCGGGGCA AGGATGGACA AGAAAGCAGA AAAAGGAGCT CCCTGTTCCG GAAGATCACC
    AAGCAGGCAT CCCTGCTCCA CACCAGCCGC AGCCTTTCTT CCCTTAACCG CTCCCTGTCG
    TCCGGCGAGA GTGGGCCAGG CTCGCCCACA CACAGCCACA GCCTTTCTCC CCGCTCTCCC
    ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC GTGCACTCAG TGGGGGGGAA TTCATCACAG
    AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT TCTCCTGCCG GCTCTGGGCA CACACGGCCG
    AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC CAACGCCAGT ACCGCTCTCC ACGGCGCAAG
    TCAGCAGGCA GCATCCCGCT GTCACCACTG GCCCACACCC CTTCACCACC ACCAGCGGCT
    TCGCCTCAGC GTTCCCCATC ACCTCTGTCT GGCCACGGGG CCCAGGCCTT CCCTACCAAG
    CTCCACTTGT CGCCTCCCCT AGGCCGGCAA CTCTCACGGC CCAAGAGCGC GGAGCCACCC
    CGCTCACCAC TACTTAAGAG GGTGCAGTCA GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT
    TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA
    AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG
    CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA GGGGTGTTGC AGGCTGCTCC CTCACGGGCC
    TTAGGGACCC TCCGCCAGGA CAGGGCTGAA CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC
    ATCCGGGAGG TTGACTCTTC AGAAGATGAT ACTGATGAGG GGCCTGAGAA CAGCCAAGGT
    GTTCAGGAGC CAAGCTTGGT ACCCCACCGA GAAGGGGGCC AGGATCCACC CCTCAGAGGA
    GCAGGAGAGG GTGAGGAAGA GGATGCCTTC TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA
    GTGGTCACAG GTCTACTGAC AGGGGTCACA CTGGGATCTC CCAGAATGGA AGGCCCTGGT
    GTCCCCCAGA AGAAGCTTGG AATCCTACAG GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC
    TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC ACAGACCCCA TCCCCGCTGA AGGCTGCTGG
    AAGGCCCAGC ACCTCCACAC CCAGGCACTG ACAGCACTTT GTCCGAGCTC ATCAGGACGC
    ATCCCTGCCA GTTGCTCCAC TGCCTCCACC CCTGGAGAGC TGGGCCCATG GTCCTGGAAA
    TTCCTCTTCA AGGGCCCTGA CAGGGCATAC CCAACCGGAA AGGCAGCAAT GGAAGATGGG
    CCAGCCAGCT CCCAAGATTT GGAAACTACA ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC
    AGACAGGAGG GGAAGTCATG GCCACCCAGT GCCCCTGGGC TGGCCCATCC ACCTTGTGAG
    GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA
    GCGCTGGGCA TCACCAAAGT GCCTGATGCC TCAGGTGACA GGAGGCAGGA CGTTCCATGC
    AAGAGCTGCC CCTTCACCCC AGAGCCTGGG CCCAGCCTGC TCCAGAAGGG CCAAGACCCA
    GGGGGCCCTC AGGAGCATCA GGACTTGGCA TTGTTACCTG ATGAGCTTTT AAAGCAAACA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003582067.2
    CDS77..5059
    Translation

    Target ORF information:

    RefSeq Version XM_003582019.4
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003582019.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGATGATT CCAGTATTCT ACAAAGGAGA GGGTTACAGA AGGAGCTGAG CCTTCCAAGA 
    AGAGGCAGCT TCTGTCGGAC AAGTAACCGC AAGAGTTTGA TTGTGACCTC TAGTACGTCA
    CCTACACTGC CACGGCCACA CTCACCACTC CATGGCCACA CAGGTAACAG TCCTTTGGAT
    AGTCCCCGGA ATTTCTCTCC AAATGCACCT GCTCACTTTT CCTTTGTTCC TGCCCGTAGC
    CATGGCCACA GAGCAGACAG GACTGATGGA CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT
    TCAGGCTATG GAACCAACAC CCCTAGTTCT ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG
    AAGCTGCACC AGTTGCCTTT CCAGCCCACG GCCGATGAGC TGCACTTCTT GACGAAGCAT
    TTCAGCACAG ACAGTGTGCC TGATGAGGAG GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC
    TCCCGCAGCC TCAGTCCTGG ACGGTCACCA GTTTCCTTTG ACAGTGAAAT AATAATGATG
    AATCATGTAT ACAAGGAAAG ATTCCCCAAG GCCACTGCAC AAATGGAAGA GCGTCTGGCC
    GAGTTCATCT CCTCCAATAC TCCAGACAAC GTGTTGCCCT TGGCTGATGG AACCTTGAGC
    TTTATTCATC ATCAGGTGAT AGAGATGGCC CGAGACTGCC TGGACAAATC CCGGAGTGGC
    CTCATTACCT CACACTACTT CTACGAACTT CAAGAGAATT TAGAGAAGCT TCTGCAAGAT
    GCTCATGAGC GCTCAGAGAG TTCAGAAGTG GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG
    ATGATTGTCA TTGCCCGCCC AGCTCGTCTC CTGGAATGCC TGGAGTTTGA CCCTGAAGAG
    TTCTACCACC TGTTAGAAGC AGCTGAGGGC CACGCCAAAG AGGGACAAGG GATTAAATGT
    GACATTCCCC GCTACATCGT TAGCCAACTG GGCCTCACAC GGGATCCCCT AGAGGAAATG
    GCCCAGTTGA GCAGCTATGA CAGTCCTGAT ACTCCGGAGA CAGATGATTC AGTCGAGGGC
    CGTGGGGCAT CTCTGACATC GAAAAAGACA CCCTCTGAGG AGGACTTCGA AACCATTAAG
    CTCATCAGCA ATGGCGCCTA TGGGGCCGTG TTCCTGGTGC GGCACAAGTC CACCCGGCAG
    CGCTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTGATCC TGCGGAACCA GATCCAGCAG
    GCCTTCGTGG AGCGGGACAT CCTGACTTTT GCTGAGAACC CCTTTGTGGT CAGCATGTTC
    TGCTCCTTTG AAACCAAGCG CCACCTGTGC ATGGTGATGG AGTATGTGGA AGGAGGAGAC
    TGTGCCACGC TGCTGAAGAA CATCGGGGCC CTGCCTGTGG ACATGGTGCG CCTGTACTTT
    GCAGAAACTG TGCTAGCCCT GGAGTACTTA CACAATTACG GCATCGTGCA CCGTGACCTC
    AAGCCTGACA ACCTGCTGAT TACATCCATG GGTCACATCA AGCTCACTGA TTTTGGCCTT
    TCCAAAATTG GCCTTATGAG TCTGACCACA AACTTGTACG AGGGCCACAT TGAAAAGGAT
    GCACGGGAGT TCCTGGACAA GCAGGTATGC GGGACCCCGG AGTACATTGC ACCTGAGGTG
    ATCCTGCGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CTATGGGCAT CATCCTGTAC
    GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT GGAGACACTC CGGAGGAGCT CTTTGGGCAA
    GTGATCAGTG ATGAGATTGT GTGGCCTGAG GGGGACGATG CACTGCCGCC GGACGCCCAA
    GATCTCACCT CAAAACTGCT CCACCAGAAC CCATTGGAGA GACTGGGCAC AGGCAGTGCC
    TATGAGGTGA AGCAGCACCC ATTCTTCACT GGTCTGGACT GGACGGGACT TCTTCGCCAG
    AAGGCTGAAT TTATTCCTCA GCTGGAGTCA GAGGATGATA CCAGCTACTT TGACACCCGC
    TCAGAGCGAT ACCACCACGT GGACTCGGAG GATGAGGAGG AAGTGAGTGA GGATGGCTGC
    CTTGAGATTC GCCAGTTCTC GTCCTGTTCT CCAAGGTTCA GCAAGGTGTA CAGCAGCATG
    GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG ACGCCACCCC CCACCAAGCG CAGCCTGAGT
    GAGGAAAAGG AGGACCGCAC GGATGGCCTG GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG
    GTGATCGGCT CCCCTGAGAT ATTACGGAAG CGGCTGTCGG TGTCCGAGTC GTCCCACACG
    GAAAGCGATT CCAGCCCTCC ACTCACCGTG CGGCGCCACT GCTCGGGCCT CCTGGACGTG
    CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG
    GGTGCATGGC TTCTGACCCC GTCTGGAGAG GGGGCGTCTG GGCCTGCCCT GGAACGGCCG
    GCCGAGCGGC GGCTGAAGCT GAATGAGGAA CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA
    GCCATGGAGA CCCGAGGAGG CCGCGGAACC CCACAGCTGG CAGAAGGAGC CACAGCCAGG
    GCCATCAGCG ACCTGGCGGT GCGCAGGGCC CGCCACCGGC TGCTCTCTGG GGACTCCATA
    GAAAAGCGCA CCGCTCGCCC CATCAACAAA GTGATCAAGT CCGCCTCAGC CACAGCCCTG
    TCCCTTCTCA TTCCTGCTGA GCACCACTCC TGCTCTCCAC TGGCCAGCCC CATGTCCCCA
    CATTCCCAGT CATCCAACCC ATCATCCAGG GACTCTTCTC CAAGCAGGGA CTTCCTGCCA
    GCCCTCAGCA GCTCACGGCC CCCCATTGTC ATCCACCGAG CCGGCAAGAA GTATGGCTTC
    ACCCTACGAG CCATCCGTGT CTACATGGGT GACTCAGATG TCTACACCGT GCACCACATG
    GTGTGGCACG TGGAGGACGG AGGTCCAGCC AGTGAGGCAG GGCTGCGTCA GGGGGACCTC
    ATCACGCACG TCAACGGGGA GCCTGTGCAC GGGCTGGTGC ACACGGAGGT GGTGGAGCTG
    ATCCTGAAGA GTGGAAACAA GGTGTCTATT TCAACAACTC CCCTGGAGAA CACCTCCATT
    AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG
    AGCCGGGGCA AGGATGGACA AGAAAGCAGA AAAAGGAGCT CCCTGTTCCG GAAGATCACC
    AAGCAGGCAT CCCTGCTCCA CACCAGCCGC AGCCTTTCTT CCCTTAACCG CTCCCTGTCG
    TCCGGCGAGA GTGGGCCAGG CTCGCCCACA CACAGCCACA GCCTTTCTCC CCGCTCTCCC
    ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC GTGCACTCAG TGGGGGGGAA TTCATCACAG
    AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT TCTCCTGCCG GCTCTGGGCA CACACGGCCG
    AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC CAACGCCAGT ACCGCTCTCC ACGGCGCAAG
    TCAGCAGGCA GCATCCCGCT GTCACCACTG GCCCACACCC CTTCACCACC ACCAGCGGCT
    TCGCCTCAGC GTTCCCCATC ACCTCTGTCT GGCCACGGGG CCCAGGCCTT CCCTACCAAG
    CTCCACTTGT CGCCTCCCCT AGGCCGGCAA CTCTCACGGC CCAAGAGCGC GGAGCCACCC
    CGCTCACCAC TACTTAAGAG GGTGCAGTCA GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT
    TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA
    AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG
    CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA GGGGTGTTGC AGGCTGCTCC CTCACGGGCC
    TTAGGGACCC TCCGCCAGGA CAGGGCTGAA CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC
    ATCCGGGAGG TTGACTCTTC AGAAGATGAT ACTGATGAGG GGCCTGAGAA CAGCCAAGGT
    GTTCAGGAGC CAAGCTTGGT ACCCCACCGA GAAGGGGGCC AGGATCCACC CCTCAGAGGA
    GCAGGAGAGG GTGAGGAAGA GGATGCCTTC TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA
    GTGGTCACAG GTCTACTGAC AGGGGTCACA CTGGGATCTC CCAGAATGGA AGGCCCTGGT
    GTCCCCCAGA AGAAGCTTGG AATCCTACAG GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC
    TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC ACAGACCCCA TCCCCGCTGA AGGCTGCTGG
    AAGGCCCAGC ACCTCCACAC CCAGGCACTG ACAGCACTTT GTCCGAGCTC ATCAGGACGC
    ATCCCTGCCA GTTGCTCCAC TGCCTCCACC CCTGGAGAGC TGGGCCCATG GTCCTGGAAA
    TTCCTCTTCA AGGGCCCTGA CAGGGCATAC CCAACCGGAA AGGCAGCAAT GGAAGATGGG
    CCAGCCAGCT CCCAAGATTT GGAAACTACA ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC
    AGACAGGAGG GGAAGTCATG GCCACCCAGT GCCCCTGGGC TGGCCCATCC ACCTTGTGAG
    GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA
    GCGCTGGGCA TCACCAAAGT GCCTGATGCC TCAGGTGACA GGAGGCAGGA CGTTCCATGC
    AAGAGCTGCC CCTTCACCCC AGAGCCTGGG CCCAGCCTGC TCCAGAAGGG CCAAGACCCA
    GGGGGCCCTC AGGAGCATCA GGACTTGGCA TTGTTACCTG ATGAGCTTTT AAAGCAAACA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53217
    Clone ID Related Accession (Same CDS sequence) XM_003582019.4 , XM_010803741.2 , XM_010803741.3
    Accession Version XM_010803741.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803741.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGATGATT CCAGTATTCT ACAAAGGAGA GGGTTACAGA AGGAGCTGAG CCTTCCAAGA 
    AGAGGCAGCT TCTGTCGGAC AAGTAACCGC AAGAGTTTGA TTGTGACCTC TAGTACGTCA
    CCTACACTGC CACGGCCACA CTCACCACTC CATGGCCACA CAGGTAACAG TCCTTTGGAT
    AGTCCCCGGA ATTTCTCTCC AAATGCACCT GCTCACTTTT CCTTTGTTCC TGCCCGTAGC
    CATGGCCACA GAGCAGACAG GACTGATGGA CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT
    TCAGGCTATG GAACCAACAC CCCTAGTTCT ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG
    AAGCTGCACC AGTTGCCTTT CCAGCCCACG GCCGATGAGC TGCACTTCTT GACGAAGCAT
    TTCAGCACAG ACAGTGTGCC TGATGAGGAG GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC
    TCCCGCAGCC TCAGTCCTGG ACGGTCACCA GTTTCCTTTG ACAGTGAAAT AATAATGATG
    AATCATGTAT ACAAGGAAAG ATTCCCCAAG GCCACTGCAC AAATGGAAGA GCGTCTGGCC
    GAGTTCATCT CCTCCAATAC TCCAGACAAC GTGTTGCCCT TGGCTGATGG AACCTTGAGC
    TTTATTCATC ATCAGGTGAT AGAGATGGCC CGAGACTGCC TGGACAAATC CCGGAGTGGC
    CTCATTACCT CACACTACTT CTACGAACTT CAAGAGAATT TAGAGAAGCT TCTGCAAGAT
    GCTCATGAGC GCTCAGAGAG TTCAGAAGTG GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG
    ATGATTGTCA TTGCCCGCCC AGCTCGTCTC CTGGAATGCC TGGAGTTTGA CCCTGAAGAG
    TTCTACCACC TGTTAGAAGC AGCTGAGGGC CACGCCAAAG AGGGACAAGG GATTAAATGT
    GACATTCCCC GCTACATCGT TAGCCAACTG GGCCTCACAC GGGATCCCCT AGAGGAAATG
    GCCCAGTTGA GCAGCTATGA CAGTCCTGAT ACTCCGGAGA CAGATGATTC AGTCGAGGGC
    CGTGGGGCAT CTCTGACATC GAAAAAGACA CCCTCTGAGG AGGACTTCGA AACCATTAAG
    CTCATCAGCA ATGGCGCCTA TGGGGCCGTG TTCCTGGTGC GGCACAAGTC CACCCGGCAG
    CGCTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTGATCC TGCGGAACCA GATCCAGCAG
    GCCTTCGTGG AGCGGGACAT CCTGACTTTT GCTGAGAACC CCTTTGTGGT CAGCATGTTC
    TGCTCCTTTG AAACCAAGCG CCACCTGTGC ATGGTGATGG AGTATGTGGA AGGAGGAGAC
    TGTGCCACGC TGCTGAAGAA CATCGGGGCC CTGCCTGTGG ACATGGTGCG CCTGTACTTT
    GCAGAAACTG TGCTAGCCCT GGAGTACTTA CACAATTACG GCATCGTGCA CCGTGACCTC
    AAGCCTGACA ACCTGCTGAT TACATCCATG GGTCACATCA AGCTCACTGA TTTTGGCCTT
    TCCAAAATTG GCCTTATGAG TCTGACCACA AACTTGTACG AGGGCCACAT TGAAAAGGAT
    GCACGGGAGT TCCTGGACAA GCAGGTATGC GGGACCCCGG AGTACATTGC ACCTGAGGTG
    ATCCTGCGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CTATGGGCAT CATCCTGTAC
    GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT GGAGACACTC CGGAGGAGCT CTTTGGGCAA
    GTGATCAGTG ATGAGATTGT GTGGCCTGAG GGGGACGATG CACTGCCGCC GGACGCCCAA
    GATCTCACCT CAAAACTGCT CCACCAGAAC CCATTGGAGA GACTGGGCAC AGGCAGTGCC
    TATGAGGTGA AGCAGCACCC ATTCTTCACT GGTCTGGACT GGACGGGACT TCTTCGCCAG
    AAGGCTGAAT TTATTCCTCA GCTGGAGTCA GAGGATGATA CCAGCTACTT TGACACCCGC
    TCAGAGCGAT ACCACCACGT GGACTCGGAG GATGAGGAGG AAGTGAGTGA GGATGGCTGC
    CTTGAGATTC GCCAGTTCTC GTCCTGTTCT CCAAGGTTCA GCAAGGTGTA CAGCAGCATG
    GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG ACGCCACCCC CCACCAAGCG CAGCCTGAGT
    GAGGAAAAGG AGGACCGCAC GGATGGCCTG GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG
    GTGATCGGCT CCCCTGAGAT ATTACGGAAG CGGCTGTCGG TGTCCGAGTC GTCCCACACG
    GAAAGCGATT CCAGCCCTCC ACTCACCGTG CGGCGCCACT GCTCGGGCCT CCTGGACGTG
    CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG
    GGTGCATGGC TTCTGACCCC GTCTGGAGAG GGGGCGTCTG GGCCTGCCCT GGAACGGCCG
    GCCGAGCGGC GGCTGAAGCT GAATGAGGAA CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA
    GCCATGGAGA CCCGAGGAGG CCGCGGAACC CCACAGCTGG CAGAAGGAGC CACAGCCAGG
    GCCATCAGCG ACCTGGCGGT GCGCAGGGCC CGCCACCGGC TGCTCTCTGG GGACTCCATA
    GAAAAGCGCA CCGCTCGCCC CATCAACAAA GTGATCAAGT CCGCCTCAGC CACAGCCCTG
    TCCCTTCTCA TTCCTGCTGA GCACCACTCC TGCTCTCCAC TGGCCAGCCC CATGTCCCCA
    CATTCCCAGT CATCCAACCC ATCATCCAGG GACTCTTCTC CAAGCAGGGA CTTCCTGCCA
    GCCCTCAGCA GCTCACGGCC CCCCATTGTC ATCCACCGAG CCGGCAAGAA GTATGGCTTC
    ACCCTACGAG CCATCCGTGT CTACATGGGT GACTCAGATG TCTACACCGT GCACCACATG
    GTGTGGCACG TGGAGGACGG AGGTCCAGCC AGTGAGGCAG GGCTGCGTCA GGGGGACCTC
    ATCACGCACG TCAACGGGGA GCCTGTGCAC GGGCTGGTGC ACACGGAGGT GGTGGAGCTG
    ATCCTGAAGA GTGGAAACAA GGTGTCTATT TCAACAACTC CCCTGGAGAA CACCTCCATT
    AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG
    AGCCGGGGCA AGGATGGACA AGAAAGCAGA AAAAGGAGCT CCCTGTTCCG GAAGATCACC
    AAGCAGGCAT CCCTGCTCCA CACCAGCCGC AGCCTTTCTT CCCTTAACCG CTCCCTGTCG
    TCCGGCGAGA GTGGGCCAGG CTCGCCCACA CACAGCCACA GCCTTTCTCC CCGCTCTCCC
    ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC GTGCACTCAG TGGGGGGGAA TTCATCACAG
    AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT TCTCCTGCCG GCTCTGGGCA CACACGGCCG
    AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC CAACGCCAGT ACCGCTCTCC ACGGCGCAAG
    TCAGCAGGCA GCATCCCGCT GTCACCACTG GCCCACACCC CTTCACCACC ACCAGCGGCT
    TCGCCTCAGC GTTCCCCATC ACCTCTGTCT GGCCACGGGG CCCAGGCCTT CCCTACCAAG
    CTCCACTTGT CGCCTCCCCT AGGCCGGCAA CTCTCACGGC CCAAGAGCGC GGAGCCACCC
    CGCTCACCAC TACTTAAGAG GGTGCAGTCA GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT
    TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA
    AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG
    CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA GGGGTGTTGC AGGCTGCTCC CTCACGGGCC
    TTAGGGACCC TCCGCCAGGA CAGGGCTGAA CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC
    ATCCGGGAGG TTGACTCTTC AGAAGATGAT ACTGATGAGG GGCCTGAGAA CAGCCAAGGT
    GTTCAGGAGC CAAGCTTGGT ACCCCACCGA GAAGGGGGCC AGGATCCACC CCTCAGAGGA
    GCAGGAGAGG GTGAGGAAGA GGATGCCTTC TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA
    GTGGTCACAG GTCTACTGAC AGGGGTCACA CTGGGATCTC CCAGAATGGA AGGCCCTGGT
    GTCCCCCAGA AGAAGCTTGG AATCCTACAG GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC
    TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC ACAGACCCCA TCCCCGCTGA AGGCTGCTGG
    AAGGCCCAGC ACCTCCACAC CCAGGCACTG ACAGCACTTT GTCCGAGCTC ATCAGGACGC
    ATCCCTGCCA GTTGCTCCAC TGCCTCCACC CCTGGAGAGC TGGGCCCATG GTCCTGGAAA
    TTCCTCTTCA AGGGCCCTGA CAGGGCATAC CCAACCGGAA AGGCAGCAAT GGAAGATGGG
    CCAGCCAGCT CCCAAGATTT GGAAACTACA ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC
    AGACAGGAGG GGAAGTCATG GCCACCCAGT GCCCCTGGGC TGGCCCATCC ACCTTGTGAG
    GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA
    GCGCTGGGCA TCACCAAAGT GCCTGATGCC TCAGGTGACA GGAGGCAGGA CGTTCCATGC
    AAGAGCTGCC CCTTCACCCC AGAGCCTGGG CCCAGCCTGC TCCAGAAGGG CCAAGACCCA
    GGGGGCCCTC AGGAGCATCA GGACTTGGCA TTGTTACCTG ATGAGCTTTT AAAGCAAACA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802043.1
    CDS77..5059
    Translation

    Target ORF information:

    RefSeq Version XM_010803741.2
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010803741.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGATGATT CCAGTATTCT ACAAAGGAGA GGGTTACAGA AGGAGCTGAG CCTTCCAAGA 
    AGAGGCAGCT TCTGTCGGAC AAGTAACCGC AAGAGTTTGA TTGTGACCTC TAGTACGTCA
    CCTACACTGC CACGGCCACA CTCACCACTC CATGGCCACA CAGGTAACAG TCCTTTGGAT
    AGTCCCCGGA ATTTCTCTCC AAATGCACCT GCTCACTTTT CCTTTGTTCC TGCCCGTAGC
    CATGGCCACA GAGCAGACAG GACTGATGGA CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT
    TCAGGCTATG GAACCAACAC CCCTAGTTCT ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG
    AAGCTGCACC AGTTGCCTTT CCAGCCCACG GCCGATGAGC TGCACTTCTT GACGAAGCAT
    TTCAGCACAG ACAGTGTGCC TGATGAGGAG GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC
    TCCCGCAGCC TCAGTCCTGG ACGGTCACCA GTTTCCTTTG ACAGTGAAAT AATAATGATG
    AATCATGTAT ACAAGGAAAG ATTCCCCAAG GCCACTGCAC AAATGGAAGA GCGTCTGGCC
    GAGTTCATCT CCTCCAATAC TCCAGACAAC GTGTTGCCCT TGGCTGATGG AACCTTGAGC
    TTTATTCATC ATCAGGTGAT AGAGATGGCC CGAGACTGCC TGGACAAATC CCGGAGTGGC
    CTCATTACCT CACACTACTT CTACGAACTT CAAGAGAATT TAGAGAAGCT TCTGCAAGAT
    GCTCATGAGC GCTCAGAGAG TTCAGAAGTG GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG
    ATGATTGTCA TTGCCCGCCC AGCTCGTCTC CTGGAATGCC TGGAGTTTGA CCCTGAAGAG
    TTCTACCACC TGTTAGAAGC AGCTGAGGGC CACGCCAAAG AGGGACAAGG GATTAAATGT
    GACATTCCCC GCTACATCGT TAGCCAACTG GGCCTCACAC GGGATCCCCT AGAGGAAATG
    GCCCAGTTGA GCAGCTATGA CAGTCCTGAT ACTCCGGAGA CAGATGATTC AGTCGAGGGC
    CGTGGGGCAT CTCTGACATC GAAAAAGACA CCCTCTGAGG AGGACTTCGA AACCATTAAG
    CTCATCAGCA ATGGCGCCTA TGGGGCCGTG TTCCTGGTGC GGCACAAGTC CACCCGGCAG
    CGCTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTGATCC TGCGGAACCA GATCCAGCAG
    GCCTTCGTGG AGCGGGACAT CCTGACTTTT GCTGAGAACC CCTTTGTGGT CAGCATGTTC
    TGCTCCTTTG AAACCAAGCG CCACCTGTGC ATGGTGATGG AGTATGTGGA AGGAGGAGAC
    TGTGCCACGC TGCTGAAGAA CATCGGGGCC CTGCCTGTGG ACATGGTGCG CCTGTACTTT
    GCAGAAACTG TGCTAGCCCT GGAGTACTTA CACAATTACG GCATCGTGCA CCGTGACCTC
    AAGCCTGACA ACCTGCTGAT TACATCCATG GGTCACATCA AGCTCACTGA TTTTGGCCTT
    TCCAAAATTG GCCTTATGAG TCTGACCACA AACTTGTACG AGGGCCACAT TGAAAAGGAT
    GCACGGGAGT TCCTGGACAA GCAGGTATGC GGGACCCCGG AGTACATTGC ACCTGAGGTG
    ATCCTGCGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CTATGGGCAT CATCCTGTAC
    GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT GGAGACACTC CGGAGGAGCT CTTTGGGCAA
    GTGATCAGTG ATGAGATTGT GTGGCCTGAG GGGGACGATG CACTGCCGCC GGACGCCCAA
    GATCTCACCT CAAAACTGCT CCACCAGAAC CCATTGGAGA GACTGGGCAC AGGCAGTGCC
    TATGAGGTGA AGCAGCACCC ATTCTTCACT GGTCTGGACT GGACGGGACT TCTTCGCCAG
    AAGGCTGAAT TTATTCCTCA GCTGGAGTCA GAGGATGATA CCAGCTACTT TGACACCCGC
    TCAGAGCGAT ACCACCACGT GGACTCGGAG GATGAGGAGG AAGTGAGTGA GGATGGCTGC
    CTTGAGATTC GCCAGTTCTC GTCCTGTTCT CCAAGGTTCA GCAAGGTGTA CAGCAGCATG
    GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG ACGCCACCCC CCACCAAGCG CAGCCTGAGT
    GAGGAAAAGG AGGACCGCAC GGATGGCCTG GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG
    GTGATCGGCT CCCCTGAGAT ATTACGGAAG CGGCTGTCGG TGTCCGAGTC GTCCCACACG
    GAAAGCGATT CCAGCCCTCC ACTCACCGTG CGGCGCCACT GCTCGGGCCT CCTGGACGTG
    CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG
    GGTGCATGGC TTCTGACCCC GTCTGGAGAG GGGGCGTCTG GGCCTGCCCT GGAACGGCCG
    GCCGAGCGGC GGCTGAAGCT GAATGAGGAA CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA
    GCCATGGAGA CCCGAGGAGG CCGCGGAACC CCACAGCTGG CAGAAGGAGC CACAGCCAGG
    GCCATCAGCG ACCTGGCGGT GCGCAGGGCC CGCCACCGGC TGCTCTCTGG GGACTCCATA
    GAAAAGCGCA CCGCTCGCCC CATCAACAAA GTGATCAAGT CCGCCTCAGC CACAGCCCTG
    TCCCTTCTCA TTCCTGCTGA GCACCACTCC TGCTCTCCAC TGGCCAGCCC CATGTCCCCA
    CATTCCCAGT CATCCAACCC ATCATCCAGG GACTCTTCTC CAAGCAGGGA CTTCCTGCCA
    GCCCTCAGCA GCTCACGGCC CCCCATTGTC ATCCACCGAG CCGGCAAGAA GTATGGCTTC
    ACCCTACGAG CCATCCGTGT CTACATGGGT GACTCAGATG TCTACACCGT GCACCACATG
    GTGTGGCACG TGGAGGACGG AGGTCCAGCC AGTGAGGCAG GGCTGCGTCA GGGGGACCTC
    ATCACGCACG TCAACGGGGA GCCTGTGCAC GGGCTGGTGC ACACGGAGGT GGTGGAGCTG
    ATCCTGAAGA GTGGAAACAA GGTGTCTATT TCAACAACTC CCCTGGAGAA CACCTCCATT
    AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG
    AGCCGGGGCA AGGATGGACA AGAAAGCAGA AAAAGGAGCT CCCTGTTCCG GAAGATCACC
    AAGCAGGCAT CCCTGCTCCA CACCAGCCGC AGCCTTTCTT CCCTTAACCG CTCCCTGTCG
    TCCGGCGAGA GTGGGCCAGG CTCGCCCACA CACAGCCACA GCCTTTCTCC CCGCTCTCCC
    ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC GTGCACTCAG TGGGGGGGAA TTCATCACAG
    AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT TCTCCTGCCG GCTCTGGGCA CACACGGCCG
    AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC CAACGCCAGT ACCGCTCTCC ACGGCGCAAG
    TCAGCAGGCA GCATCCCGCT GTCACCACTG GCCCACACCC CTTCACCACC ACCAGCGGCT
    TCGCCTCAGC GTTCCCCATC ACCTCTGTCT GGCCACGGGG CCCAGGCCTT CCCTACCAAG
    CTCCACTTGT CGCCTCCCCT AGGCCGGCAA CTCTCACGGC CCAAGAGCGC GGAGCCACCC
    CGCTCACCAC TACTTAAGAG GGTGCAGTCA GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT
    TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA
    AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG
    CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA GGGGTGTTGC AGGCTGCTCC CTCACGGGCC
    TTAGGGACCC TCCGCCAGGA CAGGGCTGAA CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC
    ATCCGGGAGG TTGACTCTTC AGAAGATGAT ACTGATGAGG GGCCTGAGAA CAGCCAAGGT
    GTTCAGGAGC CAAGCTTGGT ACCCCACCGA GAAGGGGGCC AGGATCCACC CCTCAGAGGA
    GCAGGAGAGG GTGAGGAAGA GGATGCCTTC TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA
    GTGGTCACAG GTCTACTGAC AGGGGTCACA CTGGGATCTC CCAGAATGGA AGGCCCTGGT
    GTCCCCCAGA AGAAGCTTGG AATCCTACAG GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC
    TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC ACAGACCCCA TCCCCGCTGA AGGCTGCTGG
    AAGGCCCAGC ACCTCCACAC CCAGGCACTG ACAGCACTTT GTCCGAGCTC ATCAGGACGC
    ATCCCTGCCA GTTGCTCCAC TGCCTCCACC CCTGGAGAGC TGGGCCCATG GTCCTGGAAA
    TTCCTCTTCA AGGGCCCTGA CAGGGCATAC CCAACCGGAA AGGCAGCAAT GGAAGATGGG
    CCAGCCAGCT CCCAAGATTT GGAAACTACA ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC
    AGACAGGAGG GGAAGTCATG GCCACCCAGT GCCCCTGGGC TGGCCCATCC ACCTTGTGAG
    GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA
    GCGCTGGGCA TCACCAAAGT GCCTGATGCC TCAGGTGACA GGAGGCAGGA CGTTCCATGC
    AAGAGCTGCC CCTTCACCCC AGAGCCTGGG CCCAGCCTGC TCCAGAAGGG CCAAGACCCA
    GGGGGCCCTC AGGAGCATCA GGACTTGGCA TTGTTACCTG ATGAGCTTTT AAAGCAAACA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53217
    Clone ID Related Accession (Same CDS sequence) XM_003582019.4 , XM_010803741.2 , XM_010803741.3
    Accession Version XM_010803741.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803741.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGATGATT CCAGTATTCT ACAAAGGAGA GGGTTACAGA AGGAGCTGAG CCTTCCAAGA 
    AGAGGCAGCT TCTGTCGGAC AAGTAACCGC AAGAGTTTGA TTGTGACCTC TAGTACGTCA
    CCTACACTGC CACGGCCACA CTCACCACTC CATGGCCACA CAGGTAACAG TCCTTTGGAT
    AGTCCCCGGA ATTTCTCTCC AAATGCACCT GCTCACTTTT CCTTTGTTCC TGCCCGTAGC
    CATGGCCACA GAGCAGACAG GACTGATGGA CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT
    TCAGGCTATG GAACCAACAC CCCTAGTTCT ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG
    AAGCTGCACC AGTTGCCTTT CCAGCCCACG GCCGATGAGC TGCACTTCTT GACGAAGCAT
    TTCAGCACAG ACAGTGTGCC TGATGAGGAG GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC
    TCCCGCAGCC TCAGTCCTGG ACGGTCACCA GTTTCCTTTG ACAGTGAAAT AATAATGATG
    AATCATGTAT ACAAGGAAAG ATTCCCCAAG GCCACTGCAC AAATGGAAGA GCGTCTGGCC
    GAGTTCATCT CCTCCAATAC TCCAGACAAC GTGTTGCCCT TGGCTGATGG AACCTTGAGC
    TTTATTCATC ATCAGGTGAT AGAGATGGCC CGAGACTGCC TGGACAAATC CCGGAGTGGC
    CTCATTACCT CACACTACTT CTACGAACTT CAAGAGAATT TAGAGAAGCT TCTGCAAGAT
    GCTCATGAGC GCTCAGAGAG TTCAGAAGTG GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG
    ATGATTGTCA TTGCCCGCCC AGCTCGTCTC CTGGAATGCC TGGAGTTTGA CCCTGAAGAG
    TTCTACCACC TGTTAGAAGC AGCTGAGGGC CACGCCAAAG AGGGACAAGG GATTAAATGT
    GACATTCCCC GCTACATCGT TAGCCAACTG GGCCTCACAC GGGATCCCCT AGAGGAAATG
    GCCCAGTTGA GCAGCTATGA CAGTCCTGAT ACTCCGGAGA CAGATGATTC AGTCGAGGGC
    CGTGGGGCAT CTCTGACATC GAAAAAGACA CCCTCTGAGG AGGACTTCGA AACCATTAAG
    CTCATCAGCA ATGGCGCCTA TGGGGCCGTG TTCCTGGTGC GGCACAAGTC CACCCGGCAG
    CGCTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTGATCC TGCGGAACCA GATCCAGCAG
    GCCTTCGTGG AGCGGGACAT CCTGACTTTT GCTGAGAACC CCTTTGTGGT CAGCATGTTC
    TGCTCCTTTG AAACCAAGCG CCACCTGTGC ATGGTGATGG AGTATGTGGA AGGAGGAGAC
    TGTGCCACGC TGCTGAAGAA CATCGGGGCC CTGCCTGTGG ACATGGTGCG CCTGTACTTT
    GCAGAAACTG TGCTAGCCCT GGAGTACTTA CACAATTACG GCATCGTGCA CCGTGACCTC
    AAGCCTGACA ACCTGCTGAT TACATCCATG GGTCACATCA AGCTCACTGA TTTTGGCCTT
    TCCAAAATTG GCCTTATGAG TCTGACCACA AACTTGTACG AGGGCCACAT TGAAAAGGAT
    GCACGGGAGT TCCTGGACAA GCAGGTATGC GGGACCCCGG AGTACATTGC ACCTGAGGTG
    ATCCTGCGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CTATGGGCAT CATCCTGTAC
    GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT GGAGACACTC CGGAGGAGCT CTTTGGGCAA
    GTGATCAGTG ATGAGATTGT GTGGCCTGAG GGGGACGATG CACTGCCGCC GGACGCCCAA
    GATCTCACCT CAAAACTGCT CCACCAGAAC CCATTGGAGA GACTGGGCAC AGGCAGTGCC
    TATGAGGTGA AGCAGCACCC ATTCTTCACT GGTCTGGACT GGACGGGACT TCTTCGCCAG
    AAGGCTGAAT TTATTCCTCA GCTGGAGTCA GAGGATGATA CCAGCTACTT TGACACCCGC
    TCAGAGCGAT ACCACCACGT GGACTCGGAG GATGAGGAGG AAGTGAGTGA GGATGGCTGC
    CTTGAGATTC GCCAGTTCTC GTCCTGTTCT CCAAGGTTCA GCAAGGTGTA CAGCAGCATG
    GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG ACGCCACCCC CCACCAAGCG CAGCCTGAGT
    GAGGAAAAGG AGGACCGCAC GGATGGCCTG GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG
    GTGATCGGCT CCCCTGAGAT ATTACGGAAG CGGCTGTCGG TGTCCGAGTC GTCCCACACG
    GAAAGCGATT CCAGCCCTCC ACTCACCGTG CGGCGCCACT GCTCGGGCCT CCTGGACGTG
    CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG
    GGTGCATGGC TTCTGACCCC GTCTGGAGAG GGGGCGTCTG GGCCTGCCCT GGAACGGCCG
    GCCGAGCGGC GGCTGAAGCT GAATGAGGAA CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA
    GCCATGGAGA CCCGAGGAGG CCGCGGAACC CCACAGCTGG CAGAAGGAGC CACAGCCAGG
    GCCATCAGCG ACCTGGCGGT GCGCAGGGCC CGCCACCGGC TGCTCTCTGG GGACTCCATA
    GAAAAGCGCA CCGCTCGCCC CATCAACAAA GTGATCAAGT CCGCCTCAGC CACAGCCCTG
    TCCCTTCTCA TTCCTGCTGA GCACCACTCC TGCTCTCCAC TGGCCAGCCC CATGTCCCCA
    CATTCCCAGT CATCCAACCC ATCATCCAGG GACTCTTCTC CAAGCAGGGA CTTCCTGCCA
    GCCCTCAGCA GCTCACGGCC CCCCATTGTC ATCCACCGAG CCGGCAAGAA GTATGGCTTC
    ACCCTACGAG CCATCCGTGT CTACATGGGT GACTCAGATG TCTACACCGT GCACCACATG
    GTGTGGCACG TGGAGGACGG AGGTCCAGCC AGTGAGGCAG GGCTGCGTCA GGGGGACCTC
    ATCACGCACG TCAACGGGGA GCCTGTGCAC GGGCTGGTGC ACACGGAGGT GGTGGAGCTG
    ATCCTGAAGA GTGGAAACAA GGTGTCTATT TCAACAACTC CCCTGGAGAA CACCTCCATT
    AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG
    AGCCGGGGCA AGGATGGACA AGAAAGCAGA AAAAGGAGCT CCCTGTTCCG GAAGATCACC
    AAGCAGGCAT CCCTGCTCCA CACCAGCCGC AGCCTTTCTT CCCTTAACCG CTCCCTGTCG
    TCCGGCGAGA GTGGGCCAGG CTCGCCCACA CACAGCCACA GCCTTTCTCC CCGCTCTCCC
    ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC GTGCACTCAG TGGGGGGGAA TTCATCACAG
    AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT TCTCCTGCCG GCTCTGGGCA CACACGGCCG
    AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC CAACGCCAGT ACCGCTCTCC ACGGCGCAAG
    TCAGCAGGCA GCATCCCGCT GTCACCACTG GCCCACACCC CTTCACCACC ACCAGCGGCT
    TCGCCTCAGC GTTCCCCATC ACCTCTGTCT GGCCACGGGG CCCAGGCCTT CCCTACCAAG
    CTCCACTTGT CGCCTCCCCT AGGCCGGCAA CTCTCACGGC CCAAGAGCGC GGAGCCACCC
    CGCTCACCAC TACTTAAGAG GGTGCAGTCA GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT
    TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA
    AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG
    CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA GGGGTGTTGC AGGCTGCTCC CTCACGGGCC
    TTAGGGACCC TCCGCCAGGA CAGGGCTGAA CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC
    ATCCGGGAGG TTGACTCTTC AGAAGATGAT ACTGATGAGG GGCCTGAGAA CAGCCAAGGT
    GTTCAGGAGC CAAGCTTGGT ACCCCACCGA GAAGGGGGCC AGGATCCACC CCTCAGAGGA
    GCAGGAGAGG GTGAGGAAGA GGATGCCTTC TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA
    GTGGTCACAG GTCTACTGAC AGGGGTCACA CTGGGATCTC CCAGAATGGA AGGCCCTGGT
    GTCCCCCAGA AGAAGCTTGG AATCCTACAG GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC
    TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC ACAGACCCCA TCCCCGCTGA AGGCTGCTGG
    AAGGCCCAGC ACCTCCACAC CCAGGCACTG ACAGCACTTT GTCCGAGCTC ATCAGGACGC
    ATCCCTGCCA GTTGCTCCAC TGCCTCCACC CCTGGAGAGC TGGGCCCATG GTCCTGGAAA
    TTCCTCTTCA AGGGCCCTGA CAGGGCATAC CCAACCGGAA AGGCAGCAAT GGAAGATGGG
    CCAGCCAGCT CCCAAGATTT GGAAACTACA ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC
    AGACAGGAGG GGAAGTCATG GCCACCCAGT GCCCCTGGGC TGGCCCATCC ACCTTGTGAG
    GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA
    GCGCTGGGCA TCACCAAAGT GCCTGATGCC TCAGGTGACA GGAGGCAGGA CGTTCCATGC
    AAGAGCTGCC CCTTCACCCC AGAGCCTGGG CCCAGCCTGC TCCAGAAGGG CCAAGACCCA
    GGGGGCCCTC AGGAGCATCA GGACTTGGCA TTGTTACCTG ATGAGCTTTT AAAGCAAACA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802043.1
    CDS51..5033
    Translation

    Target ORF information:

    RefSeq Version XM_010803741.3
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010803741.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGATGATT CCAGTATTCT ACAAAGGAGA GGGTTACAGA AGGAGCTGAG CCTTCCAAGA 
    AGAGGCAGCT TCTGTCGGAC AAGTAACCGC AAGAGTTTGA TTGTGACCTC TAGTACGTCA
    CCTACACTGC CACGGCCACA CTCACCACTC CATGGCCACA CAGGTAACAG TCCTTTGGAT
    AGTCCCCGGA ATTTCTCTCC AAATGCACCT GCTCACTTTT CCTTTGTTCC TGCCCGTAGC
    CATGGCCACA GAGCAGACAG GACTGATGGA CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT
    TCAGGCTATG GAACCAACAC CCCTAGTTCT ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG
    AAGCTGCACC AGTTGCCTTT CCAGCCCACG GCCGATGAGC TGCACTTCTT GACGAAGCAT
    TTCAGCACAG ACAGTGTGCC TGATGAGGAG GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC
    TCCCGCAGCC TCAGTCCTGG ACGGTCACCA GTTTCCTTTG ACAGTGAAAT AATAATGATG
    AATCATGTAT ACAAGGAAAG ATTCCCCAAG GCCACTGCAC AAATGGAAGA GCGTCTGGCC
    GAGTTCATCT CCTCCAATAC TCCAGACAAC GTGTTGCCCT TGGCTGATGG AACCTTGAGC
    TTTATTCATC ATCAGGTGAT AGAGATGGCC CGAGACTGCC TGGACAAATC CCGGAGTGGC
    CTCATTACCT CACACTACTT CTACGAACTT CAAGAGAATT TAGAGAAGCT TCTGCAAGAT
    GCTCATGAGC GCTCAGAGAG TTCAGAAGTG GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG
    ATGATTGTCA TTGCCCGCCC AGCTCGTCTC CTGGAATGCC TGGAGTTTGA CCCTGAAGAG
    TTCTACCACC TGTTAGAAGC AGCTGAGGGC CACGCCAAAG AGGGACAAGG GATTAAATGT
    GACATTCCCC GCTACATCGT TAGCCAACTG GGCCTCACAC GGGATCCCCT AGAGGAAATG
    GCCCAGTTGA GCAGCTATGA CAGTCCTGAT ACTCCGGAGA CAGATGATTC AGTCGAGGGC
    CGTGGGGCAT CTCTGACATC GAAAAAGACA CCCTCTGAGG AGGACTTCGA AACCATTAAG
    CTCATCAGCA ATGGCGCCTA TGGGGCCGTG TTCCTGGTGC GGCACAAGTC CACCCGGCAG
    CGCTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTGATCC TGCGGAACCA GATCCAGCAG
    GCCTTCGTGG AGCGGGACAT CCTGACTTTT GCTGAGAACC CCTTTGTGGT CAGCATGTTC
    TGCTCCTTTG AAACCAAGCG CCACCTGTGC ATGGTGATGG AGTATGTGGA AGGAGGAGAC
    TGTGCCACGC TGCTGAAGAA CATCGGGGCC CTGCCTGTGG ACATGGTGCG CCTGTACTTT
    GCAGAAACTG TGCTAGCCCT GGAGTACTTA CACAATTACG GCATCGTGCA CCGTGACCTC
    AAGCCTGACA ACCTGCTGAT TACATCCATG GGTCACATCA AGCTCACTGA TTTTGGCCTT
    TCCAAAATTG GCCTTATGAG TCTGACCACA AACTTGTACG AGGGCCACAT TGAAAAGGAT
    GCACGGGAGT TCCTGGACAA GCAGGTATGC GGGACCCCGG AGTACATTGC ACCTGAGGTG
    ATCCTGCGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CTATGGGCAT CATCCTGTAC
    GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT GGAGACACTC CGGAGGAGCT CTTTGGGCAA
    GTGATCAGTG ATGAGATTGT GTGGCCTGAG GGGGACGATG CACTGCCGCC GGACGCCCAA
    GATCTCACCT CAAAACTGCT CCACCAGAAC CCATTGGAGA GACTGGGCAC AGGCAGTGCC
    TATGAGGTGA AGCAGCACCC ATTCTTCACT GGTCTGGACT GGACGGGACT TCTTCGCCAG
    AAGGCTGAAT TTATTCCTCA GCTGGAGTCA GAGGATGATA CCAGCTACTT TGACACCCGC
    TCAGAGCGAT ACCACCACGT GGACTCGGAG GATGAGGAGG AAGTGAGTGA GGATGGCTGC
    CTTGAGATTC GCCAGTTCTC GTCCTGTTCT CCAAGGTTCA GCAAGGTGTA CAGCAGCATG
    GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG ACGCCACCCC CCACCAAGCG CAGCCTGAGT
    GAGGAAAAGG AGGACCGCAC GGATGGCCTG GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG
    GTGATCGGCT CCCCTGAGAT ATTACGGAAG CGGCTGTCGG TGTCCGAGTC GTCCCACACG
    GAAAGCGATT CCAGCCCTCC ACTCACCGTG CGGCGCCACT GCTCGGGCCT CCTGGACGTG
    CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG
    GGTGCATGGC TTCTGACCCC GTCTGGAGAG GGGGCGTCTG GGCCTGCCCT GGAACGGCCG
    GCCGAGCGGC GGCTGAAGCT GAATGAGGAA CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA
    GCCATGGAGA CCCGAGGAGG CCGCGGAACC CCACAGCTGG CAGAAGGAGC CACAGCCAGG
    GCCATCAGCG ACCTGGCGGT GCGCAGGGCC CGCCACCGGC TGCTCTCTGG GGACTCCATA
    GAAAAGCGCA CCGCTCGCCC CATCAACAAA GTGATCAAGT CCGCCTCAGC CACAGCCCTG
    TCCCTTCTCA TTCCTGCTGA GCACCACTCC TGCTCTCCAC TGGCCAGCCC CATGTCCCCA
    CATTCCCAGT CATCCAACCC ATCATCCAGG GACTCTTCTC CAAGCAGGGA CTTCCTGCCA
    GCCCTCAGCA GCTCACGGCC CCCCATTGTC ATCCACCGAG CCGGCAAGAA GTATGGCTTC
    ACCCTACGAG CCATCCGTGT CTACATGGGT GACTCAGATG TCTACACCGT GCACCACATG
    GTGTGGCACG TGGAGGACGG AGGTCCAGCC AGTGAGGCAG GGCTGCGTCA GGGGGACCTC
    ATCACGCACG TCAACGGGGA GCCTGTGCAC GGGCTGGTGC ACACGGAGGT GGTGGAGCTG
    ATCCTGAAGA GTGGAAACAA GGTGTCTATT TCAACAACTC CCCTGGAGAA CACCTCCATT
    AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG
    AGCCGGGGCA AGGATGGACA AGAAAGCAGA AAAAGGAGCT CCCTGTTCCG GAAGATCACC
    AAGCAGGCAT CCCTGCTCCA CACCAGCCGC AGCCTTTCTT CCCTTAACCG CTCCCTGTCG
    TCCGGCGAGA GTGGGCCAGG CTCGCCCACA CACAGCCACA GCCTTTCTCC CCGCTCTCCC
    ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC GTGCACTCAG TGGGGGGGAA TTCATCACAG
    AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT TCTCCTGCCG GCTCTGGGCA CACACGGCCG
    AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC CAACGCCAGT ACCGCTCTCC ACGGCGCAAG
    TCAGCAGGCA GCATCCCGCT GTCACCACTG GCCCACACCC CTTCACCACC ACCAGCGGCT
    TCGCCTCAGC GTTCCCCATC ACCTCTGTCT GGCCACGGGG CCCAGGCCTT CCCTACCAAG
    CTCCACTTGT CGCCTCCCCT AGGCCGGCAA CTCTCACGGC CCAAGAGCGC GGAGCCACCC
    CGCTCACCAC TACTTAAGAG GGTGCAGTCA GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT
    TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA
    AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG
    CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA GGGGTGTTGC AGGCTGCTCC CTCACGGGCC
    TTAGGGACCC TCCGCCAGGA CAGGGCTGAA CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC
    ATCCGGGAGG TTGACTCTTC AGAAGATGAT ACTGATGAGG GGCCTGAGAA CAGCCAAGGT
    GTTCAGGAGC CAAGCTTGGT ACCCCACCGA GAAGGGGGCC AGGATCCACC CCTCAGAGGA
    GCAGGAGAGG GTGAGGAAGA GGATGCCTTC TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA
    GTGGTCACAG GTCTACTGAC AGGGGTCACA CTGGGATCTC CCAGAATGGA AGGCCCTGGT
    GTCCCCCAGA AGAAGCTTGG AATCCTACAG GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC
    TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC ACAGACCCCA TCCCCGCTGA AGGCTGCTGG
    AAGGCCCAGC ACCTCCACAC CCAGGCACTG ACAGCACTTT GTCCGAGCTC ATCAGGACGC
    ATCCCTGCCA GTTGCTCCAC TGCCTCCACC CCTGGAGAGC TGGGCCCATG GTCCTGGAAA
    TTCCTCTTCA AGGGCCCTGA CAGGGCATAC CCAACCGGAA AGGCAGCAAT GGAAGATGGG
    CCAGCCAGCT CCCAAGATTT GGAAACTACA ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC
    AGACAGGAGG GGAAGTCATG GCCACCCAGT GCCCCTGGGC TGGCCCATCC ACCTTGTGAG
    GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA
    GCGCTGGGCA TCACCAAAGT GCCTGATGCC TCAGGTGACA GGAGGCAGGA CGTTCCATGC
    AAGAGCTGCC CCTTCACCCC AGAGCCTGGG CCCAGCCTGC TCCAGAAGGG CCAAGACCCA
    GGGGGCCCTC AGGAGCATCA GGACTTGGCA TTGTTACCTG ATGAGCTTTT AAAGCAAACA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18158
    Clone ID Related Accession (Same CDS sequence) XM_015462784.1 , XM_005204880.2
    Accession Version XM_015462784.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA
    CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT
    ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG
    GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG
    GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA
    GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG
    GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC
    GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC
    CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT
    CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG
    GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC
    CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC
    CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG
    GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT
    ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA
    CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG
    TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG
    AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT
    GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC
    ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC
    CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA
    CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG
    GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA
    AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC
    GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG
    GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT
    GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG
    GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC
    CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT
    GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA
    GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG
    GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT
    CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG
    ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG
    GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG
    CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG
    CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT
    GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG
    GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA
    CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC
    CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC
    CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA
    GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC
    TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG
    GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC
    ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT
    GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC
    AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC
    GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT
    TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC
    AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGCAGA
    AAAAGGAGCT CCCTGTTCCG GAAGATCACC AAGCAGGCAT CCCTGCTCCA CACCAGCCGC
    AGCCTTTCTT CCCTTAACCG CTCCCTGTCG TCCGGCGAGA GTGGGCCAGG CTCGCCCACA
    CACAGCCACA GCCTTTCTCC CCGCTCTCCC ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC
    GTGCACTCAG TGGGGGGGAA TTCATCACAG AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT
    TCTCCTGCCG GCTCTGGGCA CACACGGCCG AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC
    CAACGCCAGT ACCGCTCTCC ACGGCGCAAG TCAGCAGGCA GCATCCCGCT GTCACCACTG
    GCCCACACCC CTTCACCACC ACCAGCGGCT TCGCCTCAGC GTTCCCCATC ACCTCTGTCT
    GGCCACGGGG CCCAGGCCTT CCCTACCAAG CTCCACTTGT CGCCTCCCCT AGGCCGGCAA
    CTCTCACGGC CCAAGAGCGC GGAGCCACCC CGCTCACCAC TACTTAAGAG GGTGCAGTCA
    GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA
    CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC
    TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA
    GGGGTGTTGC AGGCTGCTCC CTCACGGGCC TTAGGGACCC TCCGCCAGGA CAGGGCTGAA
    CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC ATCCGGGAGG TTGACTCTTC AGAAGATGAT
    ACTGATGAGG GGCCTGAGAA CAGCCAAGGT GTTCAGGAGC CAAGCTTGGT ACCCCACCGA
    GAAGGGGGCC AGGATCCACC CCTCAGAGGA GCAGGAGAGG GTGAGGAAGA GGATGCCTTC
    TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA GTGGTCACAG GTCTACTGAC AGGGGTCACA
    CTGGGATCTC CCAGAATGGA AGGCCCTGGT GTCCCCCAGA AGAAGCTTGG AATCCTACAG
    GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC
    ACAGACCCCA TCCCCGCTGA AGGCTGCTGG AAGGCCCAGC ACCTCCACAC CCAGGCACTG
    ACAGCACTTT GTCCGAGCTC ATCAGGACGC ATCCCTGCCA GTTGCTCCAC TGCCTCCACC
    CCTGGAGAGC TGGGCCCATG GTCCTGGAAA TTCCTCTTCA AGGGCCCTGA CAGGGCATAC
    CCAACCGGAA AGGCAGCAAT GGAAGATGGG CCAGCCAGCT CCCAAGATTT GGAAACTACA
    ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC AGACAGGAGG GGAAGTCATG GCCACCCAGT
    GCCCCTGGGC TGGCCCATCC ACCTTGTGAG GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT
    GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA GCGCTGGGCA TCACCAAAGT GCCTGATGCC
    TCAGGTGACA GGAGGCAGGA CGTTCCATGC AAGAGCTGCC CCTTCACCCC AGAGCCTGGG
    CCCAGCCTGC TCCAGAAGGG CCAAGACCCA GGGGGCCCTC AGGAGCATCA GGACTTGGCA
    TTGTTACCTG ATGAGCTTTT AAAGCAAACA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318270.1
    CDS299..5731
    Translation

    Target ORF information:

    RefSeq Version XM_015462784.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA
    CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT
    ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG
    GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG
    GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA
    GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG
    GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC
    GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC
    CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT
    CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG
    GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC
    CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC
    CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG
    GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT
    ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA
    CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG
    TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG
    AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT
    GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC
    ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC
    CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA
    CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG
    GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA
    AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC
    GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG
    GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT
    GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG
    GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC
    CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT
    GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA
    GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG
    GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT
    CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG
    ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG
    GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG
    CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG
    CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT
    GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG
    GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA
    CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC
    CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC
    CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA
    GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC
    TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG
    GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC
    ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT
    GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC
    AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC
    GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT
    TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC
    AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGCAGA
    AAAAGGAGCT CCCTGTTCCG GAAGATCACC AAGCAGGCAT CCCTGCTCCA CACCAGCCGC
    AGCCTTTCTT CCCTTAACCG CTCCCTGTCG TCCGGCGAGA GTGGGCCAGG CTCGCCCACA
    CACAGCCACA GCCTTTCTCC CCGCTCTCCC ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC
    GTGCACTCAG TGGGGGGGAA TTCATCACAG AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT
    TCTCCTGCCG GCTCTGGGCA CACACGGCCG AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC
    CAACGCCAGT ACCGCTCTCC ACGGCGCAAG TCAGCAGGCA GCATCCCGCT GTCACCACTG
    GCCCACACCC CTTCACCACC ACCAGCGGCT TCGCCTCAGC GTTCCCCATC ACCTCTGTCT
    GGCCACGGGG CCCAGGCCTT CCCTACCAAG CTCCACTTGT CGCCTCCCCT AGGCCGGCAA
    CTCTCACGGC CCAAGAGCGC GGAGCCACCC CGCTCACCAC TACTTAAGAG GGTGCAGTCA
    GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA
    CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC
    TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA
    GGGGTGTTGC AGGCTGCTCC CTCACGGGCC TTAGGGACCC TCCGCCAGGA CAGGGCTGAA
    CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC ATCCGGGAGG TTGACTCTTC AGAAGATGAT
    ACTGATGAGG GGCCTGAGAA CAGCCAAGGT GTTCAGGAGC CAAGCTTGGT ACCCCACCGA
    GAAGGGGGCC AGGATCCACC CCTCAGAGGA GCAGGAGAGG GTGAGGAAGA GGATGCCTTC
    TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA GTGGTCACAG GTCTACTGAC AGGGGTCACA
    CTGGGATCTC CCAGAATGGA AGGCCCTGGT GTCCCCCAGA AGAAGCTTGG AATCCTACAG
    GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC
    ACAGACCCCA TCCCCGCTGA AGGCTGCTGG AAGGCCCAGC ACCTCCACAC CCAGGCACTG
    ACAGCACTTT GTCCGAGCTC ATCAGGACGC ATCCCTGCCA GTTGCTCCAC TGCCTCCACC
    CCTGGAGAGC TGGGCCCATG GTCCTGGAAA TTCCTCTTCA AGGGCCCTGA CAGGGCATAC
    CCAACCGGAA AGGCAGCAAT GGAAGATGGG CCAGCCAGCT CCCAAGATTT GGAAACTACA
    ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC AGACAGGAGG GGAAGTCATG GCCACCCAGT
    GCCCCTGGGC TGGCCCATCC ACCTTGTGAG GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT
    GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA GCGCTGGGCA TCACCAAAGT GCCTGATGCC
    TCAGGTGACA GGAGGCAGGA CGTTCCATGC AAGAGCTGCC CCTTCACCCC AGAGCCTGGG
    CCCAGCCTGC TCCAGAAGGG CCAAGACCCA GGGGGCCCTC AGGAGCATCA GGACTTGGCA
    TTGTTACCTG ATGAGCTTTT AAAGCAAACA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18158
    Clone ID Related Accession (Same CDS sequence) XM_015462784.1 , XM_005204880.2
    Accession Version XM_005204880.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005204880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA
    CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT
    ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG
    GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG
    GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA
    GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG
    GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC
    GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC
    CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT
    CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG
    GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC
    CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC
    CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG
    GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT
    ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA
    CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG
    TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG
    AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT
    GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC
    ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC
    CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA
    CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG
    GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA
    AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC
    GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG
    GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT
    GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG
    GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC
    CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT
    GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA
    GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG
    GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT
    CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG
    ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG
    GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG
    CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG
    CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT
    GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG
    GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA
    CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC
    CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC
    CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA
    GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC
    TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG
    GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC
    ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT
    GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC
    AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC
    GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT
    TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC
    AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGCAGA
    AAAAGGAGCT CCCTGTTCCG GAAGATCACC AAGCAGGCAT CCCTGCTCCA CACCAGCCGC
    AGCCTTTCTT CCCTTAACCG CTCCCTGTCG TCCGGCGAGA GTGGGCCAGG CTCGCCCACA
    CACAGCCACA GCCTTTCTCC CCGCTCTCCC ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC
    GTGCACTCAG TGGGGGGGAA TTCATCACAG AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT
    TCTCCTGCCG GCTCTGGGCA CACACGGCCG AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC
    CAACGCCAGT ACCGCTCTCC ACGGCGCAAG TCAGCAGGCA GCATCCCGCT GTCACCACTG
    GCCCACACCC CTTCACCACC ACCAGCGGCT TCGCCTCAGC GTTCCCCATC ACCTCTGTCT
    GGCCACGGGG CCCAGGCCTT CCCTACCAAG CTCCACTTGT CGCCTCCCCT AGGCCGGCAA
    CTCTCACGGC CCAAGAGCGC GGAGCCACCC CGCTCACCAC TACTTAAGAG GGTGCAGTCA
    GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA
    CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC
    TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA
    GGGGTGTTGC AGGCTGCTCC CTCACGGGCC TTAGGGACCC TCCGCCAGGA CAGGGCTGAA
    CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC ATCCGGGAGG TTGACTCTTC AGAAGATGAT
    ACTGATGAGG GGCCTGAGAA CAGCCAAGGT GTTCAGGAGC CAAGCTTGGT ACCCCACCGA
    GAAGGGGGCC AGGATCCACC CCTCAGAGGA GCAGGAGAGG GTGAGGAAGA GGATGCCTTC
    TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA GTGGTCACAG GTCTACTGAC AGGGGTCACA
    CTGGGATCTC CCAGAATGGA AGGCCCTGGT GTCCCCCAGA AGAAGCTTGG AATCCTACAG
    GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC
    ACAGACCCCA TCCCCGCTGA AGGCTGCTGG AAGGCCCAGC ACCTCCACAC CCAGGCACTG
    ACAGCACTTT GTCCGAGCTC ATCAGGACGC ATCCCTGCCA GTTGCTCCAC TGCCTCCACC
    CCTGGAGAGC TGGGCCCATG GTCCTGGAAA TTCCTCTTCA AGGGCCCTGA CAGGGCATAC
    CCAACCGGAA AGGCAGCAAT GGAAGATGGG CCAGCCAGCT CCCAAGATTT GGAAACTACA
    ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC AGACAGGAGG GGAAGTCATG GCCACCCAGT
    GCCCCTGGGC TGGCCCATCC ACCTTGTGAG GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT
    GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA GCGCTGGGCA TCACCAAAGT GCCTGATGCC
    TCAGGTGACA GGAGGCAGGA CGTTCCATGC AAGAGCTGCC CCTTCACCCC AGAGCCTGGG
    CCCAGCCTGC TCCAGAAGGG CCAAGACCCA GGGGGCCCTC AGGAGCATCA GGACTTGGCA
    TTGTTACCTG ATGAGCTTTT AAAGCAAACA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005204937.1
    CDS299..5731
    Translation

    Target ORF information:

    RefSeq Version XM_005204880.2
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204880.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA
    CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT
    ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG
    GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG
    GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA
    GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG
    GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC
    GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC
    CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT
    CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG
    GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC
    CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC
    CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG
    GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT
    ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA
    CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG
    TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG
    AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT
    GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC
    ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC
    CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA
    CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG
    GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA
    AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC
    GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG
    GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT
    GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG
    GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC
    CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT
    GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA
    GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG
    GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT
    CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG
    ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG
    GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG
    CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG
    CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT
    GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG
    GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA
    CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC
    CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC
    CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA
    GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC
    TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG
    GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC
    ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT
    GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC
    AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC
    GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT
    TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC
    AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGCAGA
    AAAAGGAGCT CCCTGTTCCG GAAGATCACC AAGCAGGCAT CCCTGCTCCA CACCAGCCGC
    AGCCTTTCTT CCCTTAACCG CTCCCTGTCG TCCGGCGAGA GTGGGCCAGG CTCGCCCACA
    CACAGCCACA GCCTTTCTCC CCGCTCTCCC ACGCAGGGCT ACCGGGTGAC CCCTGAAGCC
    GTGCACTCAG TGGGGGGGAA TTCATCACAG AGCAGCTCCC CCAGCTCCAG CGTGCCCAGT
    TCTCCTGCCG GCTCTGGGCA CACACGGCCG AGCTCCCTCC ACGGTCTGGC GCCCAAGCTC
    CAACGCCAGT ACCGCTCTCC ACGGCGCAAG TCAGCAGGCA GCATCCCGCT GTCACCACTG
    GCCCACACCC CTTCACCACC ACCAGCGGCT TCGCCTCAGC GTTCCCCATC ACCTCTGTCT
    GGCCACGGGG CCCAGGCCTT CCCTACCAAG CTCCACTTGT CGCCTCCCCT AGGCCGGCAA
    CTCTCACGGC CCAAGAGCGC GGAGCCACCC CGCTCACCAC TACTTAAGAG GGTGCAGTCA
    GCTGAAAAAC TGGCAGCTGC ACTGGCTGCT TCTGAGAAGA AGCTCGCCAC TTCTCGAAAA
    CAGAGCCTTG ACCTGCCCCA CCCCGAGCTA AAGAAGGAAT TGCCGCCCAG GGAAGTCAGC
    TCTCTGGAGG TAGTTGGGAC CAGGAGTGTG CTGTCTGGAA AGGGGGCGCT GCCAGGGAAA
    GGGGTGTTGC AGGCTGCTCC CTCACGGGCC TTAGGGACCC TCCGCCAGGA CAGGGCTGAA
    CGGCGAGAGT CGCTGCAGAA GCAGGAAGCC ATCCGGGAGG TTGACTCTTC AGAAGATGAT
    ACTGATGAGG GGCCTGAGAA CAGCCAAGGT GTTCAGGAGC CAAGCTTGGT ACCCCACCGA
    GAAGGGGGCC AGGATCCACC CCTCAGAGGA GCAGGAGAGG GTGAGGAAGA GGATGCCTTC
    TTGCCCAGGG ATCCCAGGGG ACAGGGCCCA GTGGTCACAG GTCTACTGAC AGGGGTCACA
    CTGGGATCTC CCAGAATGGA AGGCCCTGGT GTCCCCCAGA AGAAGCTTGG AATCCTACAG
    GCTTTTGAAG AGGGTTCCAG CTCCTCGTCC TCAGCCCCCA ACCTGGGGAA GGCTGGCCCC
    ACAGACCCCA TCCCCGCTGA AGGCTGCTGG AAGGCCCAGC ACCTCCACAC CCAGGCACTG
    ACAGCACTTT GTCCGAGCTC ATCAGGACGC ATCCCTGCCA GTTGCTCCAC TGCCTCCACC
    CCTGGAGAGC TGGGCCCATG GTCCTGGAAA TTCCTCTTCA AGGGCCCTGA CAGGGCATAC
    CCAACCGGAA AGGCAGCAAT GGAAGATGGG CCAGCCAGCT CCCAAGATTT GGAAACTACA
    ACTCCAGGCC ACCCTGCAAA CCTGTCCCCC AGACAGGAGG GGAAGTCATG GCCACCCAGT
    GCCCCTGGGC TGGCCCATCC ACCTTGTGAG GTTCCCAGCC AGAGCTGGCT ATGGGAGTCT
    GAGTGTGCAC AGAGAGAGAA AGAGGAGCCA GCGCTGGGCA TCACCAAAGT GCCTGATGCC
    TCAGGTGACA GGAGGCAGGA CGTTCCATGC AAGAGCTGCC CCTTCACCCC AGAGCCTGGG
    CCCAGCCTGC TCCAGAAGGG CCAAGACCCA GGGGGCCCTC AGGAGCATCA GGACTTGGCA
    TTGTTACCTG ATGAGCTTTT AAAGCAAACA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18159
    Clone ID Related Accession (Same CDS sequence) XM_005204881.2 , XM_015462785.1
    Accession Version XM_005204881.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005204881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA
    CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT
    ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG
    GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG
    GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA
    GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG
    GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC
    GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC
    CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT
    CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG
    GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC
    CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC
    CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG
    GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT
    ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA
    CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG
    TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG
    AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT
    GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC
    ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC
    CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA
    CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG
    GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA
    AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC
    GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG
    GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT
    GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG
    GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC
    CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT
    GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA
    GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG
    GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT
    CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG
    ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG
    GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG
    CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG
    CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT
    GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG
    GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA
    CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC
    CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC
    CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA
    GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC
    TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG
    GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC
    ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT
    GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC
    AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC
    GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT
    TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC
    AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGAAAA
    AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC
    CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC
    AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG
    CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT
    CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA
    CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC
    CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC
    CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC
    TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT
    GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG
    AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT
    CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG
    GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG
    CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT
    GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA
    GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG
    CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG
    GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT
    TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA
    GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA
    GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT
    GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA
    ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT
    CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC
    CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG
    TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA
    GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC
    AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG
    TTACCTGATG AGCTTTTAAA GCAAACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005204938.1
    CDS299..5728
    Translation

    Target ORF information:

    RefSeq Version XM_005204881.2
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204881.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA
    CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT
    ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG
    GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG
    GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA
    GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG
    GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC
    GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC
    CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT
    CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG
    GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC
    CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC
    CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG
    GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT
    ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA
    CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG
    TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG
    AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT
    GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC
    ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC
    CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA
    CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG
    GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA
    AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC
    GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG
    GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT
    GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG
    GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC
    CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT
    GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA
    GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG
    GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT
    CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG
    ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG
    GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG
    CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG
    CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT
    GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG
    GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA
    CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC
    CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC
    CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA
    GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC
    TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG
    GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC
    ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT
    GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC
    AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC
    GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT
    TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC
    AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGAAAA
    AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC
    CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC
    AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG
    CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT
    CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA
    CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC
    CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC
    CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC
    TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT
    GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG
    AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT
    CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG
    GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG
    CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT
    GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA
    GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG
    CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG
    GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT
    TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA
    GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA
    GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT
    GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA
    ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT
    CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC
    CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG
    TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA
    GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC
    AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG
    TTACCTGATG AGCTTTTAAA GCAAACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18159
    Clone ID Related Accession (Same CDS sequence) XM_005204881.2 , XM_015462785.1
    Accession Version XM_015462785.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA
    CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT
    ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG
    GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG
    GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA
    GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG
    GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC
    GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC
    CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT
    CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG
    GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC
    CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC
    CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG
    GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT
    ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA
    CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG
    TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG
    AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT
    GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC
    ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC
    CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA
    CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG
    GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA
    AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC
    GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG
    GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT
    GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG
    GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC
    CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT
    GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA
    GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG
    GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT
    CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG
    ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG
    GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG
    CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG
    CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT
    GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG
    GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA
    CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC
    CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC
    CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA
    GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC
    TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG
    GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC
    ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT
    GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC
    AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC
    GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT
    TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC
    AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGAAAA
    AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC
    CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC
    AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG
    CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT
    CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA
    CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC
    CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC
    CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC
    TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT
    GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG
    AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT
    CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG
    GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG
    CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT
    GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA
    GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG
    CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG
    GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT
    TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA
    GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA
    GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT
    GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA
    ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT
    CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC
    CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG
    TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA
    GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC
    AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG
    TTACCTGATG AGCTTTTAAA GCAAACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318271.1
    CDS299..5728
    Translation

    Target ORF information:

    RefSeq Version XM_015462785.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462785.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGC CATGGCCACA GAGCAGACAG GACTGATGGA
    CGGCGCTGGT CTTTGGCCTC ATTGCCTTCT TCAGGCTATG GAACCAACAC CCCTAGTTCT
    ACCGTGTCAT CGTCGTGCTC CTCTCAGGAG AAGCTGCACC AGTTGCCTTT CCAGCCCACG
    GCCGATGAGC TGCACTTCTT GACGAAGCAT TTCAGCACAG ACAGTGTGCC TGATGAGGAG
    GGGCGGCAGT CCCCCGCCCT GCGGCCCCGC TCCCGCAGCC TCAGTCCTGG ACGGTCACCA
    GTTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTAT ACAAGGAAAG ATTCCCCAAG
    GCCACTGCAC AAATGGAAGA GCGTCTGGCC GAGTTCATCT CCTCCAATAC TCCAGACAAC
    GTGTTGCCCT TGGCTGATGG AACCTTGAGC TTTATTCATC ATCAGGTGAT AGAGATGGCC
    CGAGACTGCC TGGACAAATC CCGGAGTGGC CTCATTACCT CACACTACTT CTACGAACTT
    CAAGAGAATT TAGAGAAGCT TCTGCAAGAT GCTCATGAGC GCTCAGAGAG TTCAGAAGTG
    GCTTTTGTGA TGCAGCTGGT GAAAAAGCTG ATGATTGTCA TTGCCCGCCC AGCTCGTCTC
    CTGGAATGCC TGGAGTTTGA CCCTGAAGAG TTCTACCACC TGTTAGAAGC AGCTGAGGGC
    CACGCCAAAG AGGGACAAGG GATTAAATGT GACATTCCCC GCTACATCGT TAGCCAACTG
    GGCCTCACAC GGGATCCCCT AGAGGAAATG GCCCAGTTGA GCAGCTATGA CAGTCCTGAT
    ACTCCGGAGA CAGATGATTC AGTCGAGGGC CGTGGGGCAT CTCTGACATC GAAAAAGACA
    CCCTCTGAGG AGGACTTCGA AACCATTAAG CTCATCAGCA ATGGCGCCTA TGGGGCCGTG
    TTCCTGGTGC GGCACAAGTC CACCCGGCAG CGCTTTGCCA TGAAGAAGAT CAACAAGCAG
    AACCTGATCC TGCGGAACCA GATCCAGCAG GCCTTCGTGG AGCGGGACAT CCTGACTTTT
    GCTGAGAACC CCTTTGTGGT CAGCATGTTC TGCTCCTTTG AAACCAAGCG CCACCTGTGC
    ATGGTGATGG AGTATGTGGA AGGAGGAGAC TGTGCCACGC TGCTGAAGAA CATCGGGGCC
    CTGCCTGTGG ACATGGTGCG CCTGTACTTT GCAGAAACTG TGCTAGCCCT GGAGTACTTA
    CACAATTACG GCATCGTGCA CCGTGACCTC AAGCCTGACA ACCTGCTGAT TACATCCATG
    GGTCACATCA AGCTCACTGA TTTTGGCCTT TCCAAAATTG GCCTTATGAG TCTGACCACA
    AACTTGTACG AGGGCCACAT TGAAAAGGAT GCACGGGAGT TCCTGGACAA GCAGGTATGC
    GGGACCCCGG AGTACATTGC ACCTGAGGTG ATCCTGCGGC AGGGCTACGG GAAGCCAGTG
    GACTGGTGGG CTATGGGCAT CATCCTGTAC GAGTTCCTGG TGGGCTGCGT CCCTTTCTTT
    GGAGACACTC CGGAGGAGCT CTTTGGGCAA GTGATCAGTG ATGAGATTGT GTGGCCTGAG
    GGGGACGATG CACTGCCGCC GGACGCCCAA GATCTCACCT CAAAACTGCT CCACCAGAAC
    CCATTGGAGA GACTGGGCAC AGGCAGTGCC TATGAGGTGA AGCAGCACCC ATTCTTCACT
    GGTCTGGACT GGACGGGACT TCTTCGCCAG AAGGCTGAAT TTATTCCTCA GCTGGAGTCA
    GAGGATGATA CCAGCTACTT TGACACCCGC TCAGAGCGAT ACCACCACGT GGACTCGGAG
    GATGAGGAGG AAGTGAGTGA GGATGGCTGC CTTGAGATTC GCCAGTTCTC GTCCTGTTCT
    CCAAGGTTCA GCAAGGTGTA CAGCAGCATG GAGCGGCTCT CGCTGCTCGA GGAGCGCCGG
    ACGCCACCCC CCACCAAGCG CAGCCTGAGT GAGGAAAAGG AGGACCGCAC GGATGGCCTG
    GCGGGGCTGA GGGGCCGAGA GCGAAGCTGG GTGATCGGCT CCCCTGAGAT ATTACGGAAG
    CGGCTGTCGG TGTCCGAGTC GTCCCACACG GAAAGCGATT CCAGCCCTCC ACTCACCGTG
    CGGCGCCACT GCTCGGGCCT CCTGGACGTG CCTCGCTTCC CTGAGAGCAC TGAGGAGGCT
    GTTGGCGCTC CCAGGAGGCA GCAGCAGGAG GGTGCATGGC TTCTGACCCC GTCTGGAGAG
    GGGGCGTCTG GGCCTGCCCT GGAACGGCCG GCCGAGCGGC GGCTGAAGCT GAATGAGGAA
    CCTGCTGGCC AGAGCAGCGC CCCCAGCCCA GCCATGGAGA CCCGAGGAGG CCGCGGAACC
    CCACAGCTGG CAGAAGGAGC CACAGCCAGG GCCATCAGCG ACCTGGCGGT GCGCAGGGCC
    CGCCACCGGC TGCTCTCTGG GGACTCCATA GAAAAGCGCA CCGCTCGCCC CATCAACAAA
    GTGATCAAGT CCGCCTCAGC CACAGCCCTG TCCCTTCTCA TTCCTGCTGA GCACCACTCC
    TGCTCTCCAC TGGCCAGCCC CATGTCCCCA CATTCCCAGT CATCCAACCC ATCATCCAGG
    GACTCTTCTC CAAGCAGGGA CTTCCTGCCA GCCCTCAGCA GCTCACGGCC CCCCATTGTC
    ATCCACCGAG CCGGCAAGAA GTATGGCTTC ACCCTACGAG CCATCCGTGT CTACATGGGT
    GACTCAGATG TCTACACCGT GCACCACATG GTGTGGCACG TGGAGGACGG AGGTCCAGCC
    AGTGAGGCAG GGCTGCGTCA GGGGGACCTC ATCACGCACG TCAACGGGGA GCCTGTGCAC
    GGGCTGGTGC ACACGGAGGT GGTGGAGCTG ATCCTGAAGA GTGGAAACAA GGTGTCTATT
    TCAACAACTC CCCTGGAGAA CACCTCCATT AAGGTGGGGC CCGCTCGGAA GGGCAGCTAC
    AAGGCCAAGA TGGCCCGAAG GAGCAAGCGG AGCCGGGGCA AGGATGGACA AGAAAGAAAA
    AGGAGCTCCC TGTTCCGGAA GATCACCAAG CAGGCATCCC TGCTCCACAC CAGCCGCAGC
    CTTTCTTCCC TTAACCGCTC CCTGTCGTCC GGCGAGAGTG GGCCAGGCTC GCCCACACAC
    AGCCACAGCC TTTCTCCCCG CTCTCCCACG CAGGGCTACC GGGTGACCCC TGAAGCCGTG
    CACTCAGTGG GGGGGAATTC ATCACAGAGC AGCTCCCCCA GCTCCAGCGT GCCCAGTTCT
    CCTGCCGGCT CTGGGCACAC ACGGCCGAGC TCCCTCCACG GTCTGGCGCC CAAGCTCCAA
    CGCCAGTACC GCTCTCCACG GCGCAAGTCA GCAGGCAGCA TCCCGCTGTC ACCACTGGCC
    CACACCCCTT CACCACCACC AGCGGCTTCG CCTCAGCGTT CCCCATCACC TCTGTCTGGC
    CACGGGGCCC AGGCCTTCCC TACCAAGCTC CACTTGTCGC CTCCCCTAGG CCGGCAACTC
    TCACGGCCCA AGAGCGCGGA GCCACCCCGC TCACCACTAC TTAAGAGGGT GCAGTCAGCT
    GAAAAACTGG CAGCTGCACT GGCTGCTTCT GAGAAGAAGC TCGCCACTTC TCGAAAACAG
    AGCCTTGACC TGCCCCACCC CGAGCTAAAG AAGGAATTGC CGCCCAGGGA AGTCAGCTCT
    CTGGAGGTAG TTGGGACCAG GAGTGTGCTG TCTGGAAAGG GGGCGCTGCC AGGGAAAGGG
    GTGTTGCAGG CTGCTCCCTC ACGGGCCTTA GGGACCCTCC GCCAGGACAG GGCTGAACGG
    CGAGAGTCGC TGCAGAAGCA GGAAGCCATC CGGGAGGTTG ACTCTTCAGA AGATGATACT
    GATGAGGGGC CTGAGAACAG CCAAGGTGTT CAGGAGCCAA GCTTGGTACC CCACCGAGAA
    GGGGGCCAGG ATCCACCCCT CAGAGGAGCA GGAGAGGGTG AGGAAGAGGA TGCCTTCTTG
    CCCAGGGATC CCAGGGGACA GGGCCCAGTG GTCACAGGTC TACTGACAGG GGTCACACTG
    GGATCTCCCA GAATGGAAGG CCCTGGTGTC CCCCAGAAGA AGCTTGGAAT CCTACAGGCT
    TTTGAAGAGG GTTCCAGCTC CTCGTCCTCA GCCCCCAACC TGGGGAAGGC TGGCCCCACA
    GACCCCATCC CCGCTGAAGG CTGCTGGAAG GCCCAGCACC TCCACACCCA GGCACTGACA
    GCACTTTGTC CGAGCTCATC AGGACGCATC CCTGCCAGTT GCTCCACTGC CTCCACCCCT
    GGAGAGCTGG GCCCATGGTC CTGGAAATTC CTCTTCAAGG GCCCTGACAG GGCATACCCA
    ACCGGAAAGG CAGCAATGGA AGATGGGCCA GCCAGCTCCC AAGATTTGGA AACTACAACT
    CCAGGCCACC CTGCAAACCT GTCCCCCAGA CAGGAGGGGA AGTCATGGCC ACCCAGTGCC
    CCTGGGCTGG CCCATCCACC TTGTGAGGTT CCCAGCCAGA GCTGGCTATG GGAGTCTGAG
    TGTGCACAGA GAGAGAAAGA GGAGCCAGCG CTGGGCATCA CCAAAGTGCC TGATGCCTCA
    GGTGACAGGA GGCAGGACGT TCCATGCAAG AGCTGCCCCT TCACCCCAGA GCCTGGGCCC
    AGCCTGCTCC AGAAGGGCCA AGACCCAGGG GGCCCTCAGG AGCATCAGGA CTTGGCATTG
    TTACCTGATG AGCTTTTAAA GCAAACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18160
    Clone ID Related Accession (Same CDS sequence) XM_003585907.3 , XM_015462786.1
    Accession Version XM_003585907.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_003585907.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA
    TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC
    TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG
    ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG
    CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA
    ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG
    CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA
    ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC
    CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT
    CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG
    AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC
    CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG
    ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA
    GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA
    GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA
    ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC
    ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG
    ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC
    AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA
    GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC
    CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC
    CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT
    TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT
    GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA
    CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC
    ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC
    TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG
    GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA
    GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT
    CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT
    GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG
    GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC
    AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC
    AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG
    CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG
    TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC
    CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG
    CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG
    GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC
    CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC
    ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG
    GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC
    ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC
    ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC
    TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG
    TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG
    CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG
    GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG
    GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC
    ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG
    AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG
    AAGATCACCA AGCAGGCATC CCTGCTCCAC ACCAGCCGCA GCCTTTCTTC CCTTAACCGC
    TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC TCGCCCACAC ACAGCCACAG CCTTTCTCCC
    CGCTCTCCCA CGCAGGGCTA CCGGGTGACC CCTGAAGCCG TGCACTCAGT GGGGGGGAAT
    TCATCACAGA GCAGCTCCCC CAGCTCCAGC GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC
    ACACGGCCGA GCTCCCTCCA CGGTCTGGCG CCCAAGCTCC AACGCCAGTA CCGCTCTCCA
    CGGCGCAAGT CAGCAGGCAG CATCCCGCTG TCACCACTGG CCCACACCCC TTCACCACCA
    CCAGCGGCTT CGCCTCAGCG TTCCCCATCA CCTCTGTCTG GCCACGGGGC CCAGGCCTTC
    CCTACCAAGC TCCACTTGTC GCCTCCCCTA GGCCGGCAAC TCTCACGGCC CAAGAGCGCG
    GAGCCACCCC GCTCACCACT ACTTAAGAGG GTGCAGTCAG CTGAAAAACT GGCAGCTGCA
    CTGGCTGCTT CTGAGAAGAA GCTCGCCACT TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC
    CCCGAGCTAA AGAAGGAATT GCCGCCCAGG GAAGTCAGCT CTCTGGAGGT AGTTGGGACC
    AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC
    TCACGGGCCT TAGGGACCCT CCGCCAGGAC AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG
    CAGGAAGCCA TCCGGGAGGT TGACTCTTCA GAAGATGATA CTGATGAGGG GCCTGAGAAC
    AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA CCCCACCGAG AAGGGGGCCA GGATCCACCC
    CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG GATGCCTTCT TGCCCAGGGA TCCCAGGGGA
    CAGGGCCCAG TGGTCACAGG TCTACTGACA GGGGTCACAC TGGGATCTCC CAGAATGGAA
    GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA ATCCTACAGG CTTTTGAAGA GGGTTCCAGC
    TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG GCTGGCCCCA CAGACCCCAT CCCCGCTGAA
    GGCTGCTGGA AGGCCCAGCA CCTCCACACC CAGGCACTGA CAGCACTTTG TCCGAGCTCA
    TCAGGACGCA TCCCTGCCAG TTGCTCCACT GCCTCCACCC CTGGAGAGCT GGGCCCATGG
    TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC AGGGCATACC CAACCGGAAA GGCAGCAATG
    GAAGATGGGC CAGCCAGCTC CCAAGATTTG GAAACTACAA CTCCAGGCCA CCCTGCAAAC
    CTGTCCCCCA GACAGGAGGG GAAGTCATGG CCACCCAGTG CCCCTGGGCT GGCCCATCCA
    CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA TGGGAGTCTG AGTGTGCACA GAGAGAGAAA
    GAGGAGCCAG CGCTGGGCAT CACCAAAGTG CCTGATGCCT CAGGTGACAG GAGGCAGGAC
    GTTCCATGCA AGAGCTGCCC CTTCACCCCA GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC
    CAAGACCCAG GGGGCCCTCA GGAGCATCAG GACTTGGCAT TGTTACCTGA TGAGCTTTTA
    AAGCAAACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003585955.1
    CDS299..5710
    Translation

    Target ORF information:

    RefSeq Version XM_003585907.3
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003585907.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA
    TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC
    TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG
    ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG
    CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA
    ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG
    CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA
    ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC
    CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT
    CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG
    AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC
    CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG
    ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA
    GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA
    GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA
    ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC
    ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG
    ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC
    AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA
    GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC
    CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC
    CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT
    TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT
    GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA
    CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC
    ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC
    TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG
    GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA
    GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT
    CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT
    GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG
    GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC
    AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC
    AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG
    CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG
    TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC
    CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG
    CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG
    GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC
    CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC
    ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG
    GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC
    ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC
    ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC
    TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG
    TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG
    CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG
    GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG
    GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC
    ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG
    AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG
    AAGATCACCA AGCAGGCATC CCTGCTCCAC ACCAGCCGCA GCCTTTCTTC CCTTAACCGC
    TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC TCGCCCACAC ACAGCCACAG CCTTTCTCCC
    CGCTCTCCCA CGCAGGGCTA CCGGGTGACC CCTGAAGCCG TGCACTCAGT GGGGGGGAAT
    TCATCACAGA GCAGCTCCCC CAGCTCCAGC GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC
    ACACGGCCGA GCTCCCTCCA CGGTCTGGCG CCCAAGCTCC AACGCCAGTA CCGCTCTCCA
    CGGCGCAAGT CAGCAGGCAG CATCCCGCTG TCACCACTGG CCCACACCCC TTCACCACCA
    CCAGCGGCTT CGCCTCAGCG TTCCCCATCA CCTCTGTCTG GCCACGGGGC CCAGGCCTTC
    CCTACCAAGC TCCACTTGTC GCCTCCCCTA GGCCGGCAAC TCTCACGGCC CAAGAGCGCG
    GAGCCACCCC GCTCACCACT ACTTAAGAGG GTGCAGTCAG CTGAAAAACT GGCAGCTGCA
    CTGGCTGCTT CTGAGAAGAA GCTCGCCACT TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC
    CCCGAGCTAA AGAAGGAATT GCCGCCCAGG GAAGTCAGCT CTCTGGAGGT AGTTGGGACC
    AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC
    TCACGGGCCT TAGGGACCCT CCGCCAGGAC AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG
    CAGGAAGCCA TCCGGGAGGT TGACTCTTCA GAAGATGATA CTGATGAGGG GCCTGAGAAC
    AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA CCCCACCGAG AAGGGGGCCA GGATCCACCC
    CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG GATGCCTTCT TGCCCAGGGA TCCCAGGGGA
    CAGGGCCCAG TGGTCACAGG TCTACTGACA GGGGTCACAC TGGGATCTCC CAGAATGGAA
    GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA ATCCTACAGG CTTTTGAAGA GGGTTCCAGC
    TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG GCTGGCCCCA CAGACCCCAT CCCCGCTGAA
    GGCTGCTGGA AGGCCCAGCA CCTCCACACC CAGGCACTGA CAGCACTTTG TCCGAGCTCA
    TCAGGACGCA TCCCTGCCAG TTGCTCCACT GCCTCCACCC CTGGAGAGCT GGGCCCATGG
    TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC AGGGCATACC CAACCGGAAA GGCAGCAATG
    GAAGATGGGC CAGCCAGCTC CCAAGATTTG GAAACTACAA CTCCAGGCCA CCCTGCAAAC
    CTGTCCCCCA GACAGGAGGG GAAGTCATGG CCACCCAGTG CCCCTGGGCT GGCCCATCCA
    CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA TGGGAGTCTG AGTGTGCACA GAGAGAGAAA
    GAGGAGCCAG CGCTGGGCAT CACCAAAGTG CCTGATGCCT CAGGTGACAG GAGGCAGGAC
    GTTCCATGCA AGAGCTGCCC CTTCACCCCA GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC
    CAAGACCCAG GGGGCCCTCA GGAGCATCAG GACTTGGCAT TGTTACCTGA TGAGCTTTTA
    AAGCAAACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18160
    Clone ID Related Accession (Same CDS sequence) XM_003585907.3 , XM_015462786.1
    Accession Version XM_015462786.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA
    TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC
    TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG
    ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG
    CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA
    ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG
    CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA
    ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC
    CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT
    CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG
    AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC
    CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG
    ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA
    GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA
    GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA
    ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC
    ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG
    ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC
    AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA
    GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC
    CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC
    CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT
    TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT
    GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA
    CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC
    ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC
    TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG
    GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA
    GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT
    CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT
    GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG
    GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC
    AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC
    AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG
    CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG
    TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC
    CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG
    CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG
    GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC
    CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC
    ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG
    GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC
    ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC
    ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC
    TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG
    TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG
    CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG
    GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG
    GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC
    ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG
    AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG
    AAGATCACCA AGCAGGCATC CCTGCTCCAC ACCAGCCGCA GCCTTTCTTC CCTTAACCGC
    TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC TCGCCCACAC ACAGCCACAG CCTTTCTCCC
    CGCTCTCCCA CGCAGGGCTA CCGGGTGACC CCTGAAGCCG TGCACTCAGT GGGGGGGAAT
    TCATCACAGA GCAGCTCCCC CAGCTCCAGC GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC
    ACACGGCCGA GCTCCCTCCA CGGTCTGGCG CCCAAGCTCC AACGCCAGTA CCGCTCTCCA
    CGGCGCAAGT CAGCAGGCAG CATCCCGCTG TCACCACTGG CCCACACCCC TTCACCACCA
    CCAGCGGCTT CGCCTCAGCG TTCCCCATCA CCTCTGTCTG GCCACGGGGC CCAGGCCTTC
    CCTACCAAGC TCCACTTGTC GCCTCCCCTA GGCCGGCAAC TCTCACGGCC CAAGAGCGCG
    GAGCCACCCC GCTCACCACT ACTTAAGAGG GTGCAGTCAG CTGAAAAACT GGCAGCTGCA
    CTGGCTGCTT CTGAGAAGAA GCTCGCCACT TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC
    CCCGAGCTAA AGAAGGAATT GCCGCCCAGG GAAGTCAGCT CTCTGGAGGT AGTTGGGACC
    AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC
    TCACGGGCCT TAGGGACCCT CCGCCAGGAC AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG
    CAGGAAGCCA TCCGGGAGGT TGACTCTTCA GAAGATGATA CTGATGAGGG GCCTGAGAAC
    AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA CCCCACCGAG AAGGGGGCCA GGATCCACCC
    CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG GATGCCTTCT TGCCCAGGGA TCCCAGGGGA
    CAGGGCCCAG TGGTCACAGG TCTACTGACA GGGGTCACAC TGGGATCTCC CAGAATGGAA
    GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA ATCCTACAGG CTTTTGAAGA GGGTTCCAGC
    TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG GCTGGCCCCA CAGACCCCAT CCCCGCTGAA
    GGCTGCTGGA AGGCCCAGCA CCTCCACACC CAGGCACTGA CAGCACTTTG TCCGAGCTCA
    TCAGGACGCA TCCCTGCCAG TTGCTCCACT GCCTCCACCC CTGGAGAGCT GGGCCCATGG
    TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC AGGGCATACC CAACCGGAAA GGCAGCAATG
    GAAGATGGGC CAGCCAGCTC CCAAGATTTG GAAACTACAA CTCCAGGCCA CCCTGCAAAC
    CTGTCCCCCA GACAGGAGGG GAAGTCATGG CCACCCAGTG CCCCTGGGCT GGCCCATCCA
    CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA TGGGAGTCTG AGTGTGCACA GAGAGAGAAA
    GAGGAGCCAG CGCTGGGCAT CACCAAAGTG CCTGATGCCT CAGGTGACAG GAGGCAGGAC
    GTTCCATGCA AGAGCTGCCC CTTCACCCCA GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC
    CAAGACCCAG GGGGCCCTCA GGAGCATCAG GACTTGGCAT TGTTACCTGA TGAGCTTTTA
    AAGCAAACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318272.1
    CDS299..5710
    Translation

    Target ORF information:

    RefSeq Version XM_015462786.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462786.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA
    TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC
    TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG
    ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG
    CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA
    ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG
    CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA
    ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC
    CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT
    CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG
    AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC
    CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG
    ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA
    GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA
    GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA
    ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC
    ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG
    ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC
    AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA
    GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC
    CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC
    CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT
    TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT
    GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA
    CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC
    ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC
    TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG
    GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA
    GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT
    CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT
    GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG
    GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC
    AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC
    AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG
    CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG
    TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC
    CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG
    CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG
    GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC
    CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC
    ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG
    GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC
    ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC
    ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC
    TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG
    TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG
    CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG
    GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG
    GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC
    ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG
    AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGCAGAA AAAGGAGCTC CCTGTTCCGG
    AAGATCACCA AGCAGGCATC CCTGCTCCAC ACCAGCCGCA GCCTTTCTTC CCTTAACCGC
    TCCCTGTCGT CCGGCGAGAG TGGGCCAGGC TCGCCCACAC ACAGCCACAG CCTTTCTCCC
    CGCTCTCCCA CGCAGGGCTA CCGGGTGACC CCTGAAGCCG TGCACTCAGT GGGGGGGAAT
    TCATCACAGA GCAGCTCCCC CAGCTCCAGC GTGCCCAGTT CTCCTGCCGG CTCTGGGCAC
    ACACGGCCGA GCTCCCTCCA CGGTCTGGCG CCCAAGCTCC AACGCCAGTA CCGCTCTCCA
    CGGCGCAAGT CAGCAGGCAG CATCCCGCTG TCACCACTGG CCCACACCCC TTCACCACCA
    CCAGCGGCTT CGCCTCAGCG TTCCCCATCA CCTCTGTCTG GCCACGGGGC CCAGGCCTTC
    CCTACCAAGC TCCACTTGTC GCCTCCCCTA GGCCGGCAAC TCTCACGGCC CAAGAGCGCG
    GAGCCACCCC GCTCACCACT ACTTAAGAGG GTGCAGTCAG CTGAAAAACT GGCAGCTGCA
    CTGGCTGCTT CTGAGAAGAA GCTCGCCACT TCTCGAAAAC AGAGCCTTGA CCTGCCCCAC
    CCCGAGCTAA AGAAGGAATT GCCGCCCAGG GAAGTCAGCT CTCTGGAGGT AGTTGGGACC
    AGGAGTGTGC TGTCTGGAAA GGGGGCGCTG CCAGGGAAAG GGGTGTTGCA GGCTGCTCCC
    TCACGGGCCT TAGGGACCCT CCGCCAGGAC AGGGCTGAAC GGCGAGAGTC GCTGCAGAAG
    CAGGAAGCCA TCCGGGAGGT TGACTCTTCA GAAGATGATA CTGATGAGGG GCCTGAGAAC
    AGCCAAGGTG TTCAGGAGCC AAGCTTGGTA CCCCACCGAG AAGGGGGCCA GGATCCACCC
    CTCAGAGGAG CAGGAGAGGG TGAGGAAGAG GATGCCTTCT TGCCCAGGGA TCCCAGGGGA
    CAGGGCCCAG TGGTCACAGG TCTACTGACA GGGGTCACAC TGGGATCTCC CAGAATGGAA
    GGCCCTGGTG TCCCCCAGAA GAAGCTTGGA ATCCTACAGG CTTTTGAAGA GGGTTCCAGC
    TCCTCGTCCT CAGCCCCCAA CCTGGGGAAG GCTGGCCCCA CAGACCCCAT CCCCGCTGAA
    GGCTGCTGGA AGGCCCAGCA CCTCCACACC CAGGCACTGA CAGCACTTTG TCCGAGCTCA
    TCAGGACGCA TCCCTGCCAG TTGCTCCACT GCCTCCACCC CTGGAGAGCT GGGCCCATGG
    TCCTGGAAAT TCCTCTTCAA GGGCCCTGAC AGGGCATACC CAACCGGAAA GGCAGCAATG
    GAAGATGGGC CAGCCAGCTC CCAAGATTTG GAAACTACAA CTCCAGGCCA CCCTGCAAAC
    CTGTCCCCCA GACAGGAGGG GAAGTCATGG CCACCCAGTG CCCCTGGGCT GGCCCATCCA
    CCTTGTGAGG TTCCCAGCCA GAGCTGGCTA TGGGAGTCTG AGTGTGCACA GAGAGAGAAA
    GAGGAGCCAG CGCTGGGCAT CACCAAAGTG CCTGATGCCT CAGGTGACAG GAGGCAGGAC
    GTTCCATGCA AGAGCTGCCC CTTCACCCCA GAGCCTGGGC CCAGCCTGCT CCAGAAGGGC
    CAAGACCCAG GGGGCCCTCA GGAGCATCAG GACTTGGCAT TGTTACCTGA TGAGCTTTTA
    AAGCAAACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18161
    Clone ID Related Accession (Same CDS sequence) XM_015462787.1 , XM_005204882.2
    Accession Version XM_015462787.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA
    TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC
    TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG
    ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG
    CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA
    ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG
    CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA
    ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC
    CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT
    CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG
    AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC
    CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG
    ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA
    GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA
    GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA
    ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC
    ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG
    ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC
    AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA
    GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC
    CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC
    CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT
    TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT
    GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA
    CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC
    ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC
    TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG
    GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA
    GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT
    CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT
    GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG
    GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC
    AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC
    AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG
    CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG
    TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC
    CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG
    CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG
    GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC
    CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC
    ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG
    GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC
    ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC
    ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC
    TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG
    TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG
    CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG
    GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG
    GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC
    ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG
    AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGAAAAA GGAGCTCCCT GTTCCGGAAG
    ATCACCAAGC AGGCATCCCT GCTCCACACC AGCCGCAGCC TTTCTTCCCT TAACCGCTCC
    CTGTCGTCCG GCGAGAGTGG GCCAGGCTCG CCCACACACA GCCACAGCCT TTCTCCCCGC
    TCTCCCACGC AGGGCTACCG GGTGACCCCT GAAGCCGTGC ACTCAGTGGG GGGGAATTCA
    TCACAGAGCA GCTCCCCCAG CTCCAGCGTG CCCAGTTCTC CTGCCGGCTC TGGGCACACA
    CGGCCGAGCT CCCTCCACGG TCTGGCGCCC AAGCTCCAAC GCCAGTACCG CTCTCCACGG
    CGCAAGTCAG CAGGCAGCAT CCCGCTGTCA CCACTGGCCC ACACCCCTTC ACCACCACCA
    GCGGCTTCGC CTCAGCGTTC CCCATCACCT CTGTCTGGCC ACGGGGCCCA GGCCTTCCCT
    ACCAAGCTCC ACTTGTCGCC TCCCCTAGGC CGGCAACTCT CACGGCCCAA GAGCGCGGAG
    CCACCCCGCT CACCACTACT TAAGAGGGTG CAGTCAGCTG AAAAACTGGC AGCTGCACTG
    GCTGCTTCTG AGAAGAAGCT CGCCACTTCT CGAAAACAGA GCCTTGACCT GCCCCACCCC
    GAGCTAAAGA AGGAATTGCC GCCCAGGGAA GTCAGCTCTC TGGAGGTAGT TGGGACCAGG
    AGTGTGCTGT CTGGAAAGGG GGCGCTGCCA GGGAAAGGGG TGTTGCAGGC TGCTCCCTCA
    CGGGCCTTAG GGACCCTCCG CCAGGACAGG GCTGAACGGC GAGAGTCGCT GCAGAAGCAG
    GAAGCCATCC GGGAGGTTGA CTCTTCAGAA GATGATACTG ATGAGGGGCC TGAGAACAGC
    CAAGGTGTTC AGGAGCCAAG CTTGGTACCC CACCGAGAAG GGGGCCAGGA TCCACCCCTC
    AGAGGAGCAG GAGAGGGTGA GGAAGAGGAT GCCTTCTTGC CCAGGGATCC CAGGGGACAG
    GGCCCAGTGG TCACAGGTCT ACTGACAGGG GTCACACTGG GATCTCCCAG AATGGAAGGC
    CCTGGTGTCC CCCAGAAGAA GCTTGGAATC CTACAGGCTT TTGAAGAGGG TTCCAGCTCC
    TCGTCCTCAG CCCCCAACCT GGGGAAGGCT GGCCCCACAG ACCCCATCCC CGCTGAAGGC
    TGCTGGAAGG CCCAGCACCT CCACACCCAG GCACTGACAG CACTTTGTCC GAGCTCATCA
    GGACGCATCC CTGCCAGTTG CTCCACTGCC TCCACCCCTG GAGAGCTGGG CCCATGGTCC
    TGGAAATTCC TCTTCAAGGG CCCTGACAGG GCATACCCAA CCGGAAAGGC AGCAATGGAA
    GATGGGCCAG CCAGCTCCCA AGATTTGGAA ACTACAACTC CAGGCCACCC TGCAAACCTG
    TCCCCCAGAC AGGAGGGGAA GTCATGGCCA CCCAGTGCCC CTGGGCTGGC CCATCCACCT
    TGTGAGGTTC CCAGCCAGAG CTGGCTATGG GAGTCTGAGT GTGCACAGAG AGAGAAAGAG
    GAGCCAGCGC TGGGCATCAC CAAAGTGCCT GATGCCTCAG GTGACAGGAG GCAGGACGTT
    CCATGCAAGA GCTGCCCCTT CACCCCAGAG CCTGGGCCCA GCCTGCTCCA GAAGGGCCAA
    GACCCAGGGG GCCCTCAGGA GCATCAGGAC TTGGCATTGT TACCTGATGA GCTTTTAAAG
    CAAACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318273.1
    CDS299..5707
    Translation

    Target ORF information:

    RefSeq Version XM_015462787.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462787.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA
    TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC
    TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG
    ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG
    CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA
    ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG
    CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA
    ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC
    CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT
    CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG
    AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC
    CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG
    ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA
    GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA
    GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA
    ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC
    ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG
    ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC
    AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA
    GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC
    CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC
    CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT
    TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT
    GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA
    CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC
    ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC
    TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG
    GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA
    GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT
    CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT
    GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG
    GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC
    AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC
    AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG
    CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG
    TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC
    CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG
    CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG
    GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC
    CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC
    ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG
    GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC
    ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC
    ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC
    TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG
    TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG
    CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG
    GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG
    GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC
    ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG
    AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGAAAAA GGAGCTCCCT GTTCCGGAAG
    ATCACCAAGC AGGCATCCCT GCTCCACACC AGCCGCAGCC TTTCTTCCCT TAACCGCTCC
    CTGTCGTCCG GCGAGAGTGG GCCAGGCTCG CCCACACACA GCCACAGCCT TTCTCCCCGC
    TCTCCCACGC AGGGCTACCG GGTGACCCCT GAAGCCGTGC ACTCAGTGGG GGGGAATTCA
    TCACAGAGCA GCTCCCCCAG CTCCAGCGTG CCCAGTTCTC CTGCCGGCTC TGGGCACACA
    CGGCCGAGCT CCCTCCACGG TCTGGCGCCC AAGCTCCAAC GCCAGTACCG CTCTCCACGG
    CGCAAGTCAG CAGGCAGCAT CCCGCTGTCA CCACTGGCCC ACACCCCTTC ACCACCACCA
    GCGGCTTCGC CTCAGCGTTC CCCATCACCT CTGTCTGGCC ACGGGGCCCA GGCCTTCCCT
    ACCAAGCTCC ACTTGTCGCC TCCCCTAGGC CGGCAACTCT CACGGCCCAA GAGCGCGGAG
    CCACCCCGCT CACCACTACT TAAGAGGGTG CAGTCAGCTG AAAAACTGGC AGCTGCACTG
    GCTGCTTCTG AGAAGAAGCT CGCCACTTCT CGAAAACAGA GCCTTGACCT GCCCCACCCC
    GAGCTAAAGA AGGAATTGCC GCCCAGGGAA GTCAGCTCTC TGGAGGTAGT TGGGACCAGG
    AGTGTGCTGT CTGGAAAGGG GGCGCTGCCA GGGAAAGGGG TGTTGCAGGC TGCTCCCTCA
    CGGGCCTTAG GGACCCTCCG CCAGGACAGG GCTGAACGGC GAGAGTCGCT GCAGAAGCAG
    GAAGCCATCC GGGAGGTTGA CTCTTCAGAA GATGATACTG ATGAGGGGCC TGAGAACAGC
    CAAGGTGTTC AGGAGCCAAG CTTGGTACCC CACCGAGAAG GGGGCCAGGA TCCACCCCTC
    AGAGGAGCAG GAGAGGGTGA GGAAGAGGAT GCCTTCTTGC CCAGGGATCC CAGGGGACAG
    GGCCCAGTGG TCACAGGTCT ACTGACAGGG GTCACACTGG GATCTCCCAG AATGGAAGGC
    CCTGGTGTCC CCCAGAAGAA GCTTGGAATC CTACAGGCTT TTGAAGAGGG TTCCAGCTCC
    TCGTCCTCAG CCCCCAACCT GGGGAAGGCT GGCCCCACAG ACCCCATCCC CGCTGAAGGC
    TGCTGGAAGG CCCAGCACCT CCACACCCAG GCACTGACAG CACTTTGTCC GAGCTCATCA
    GGACGCATCC CTGCCAGTTG CTCCACTGCC TCCACCCCTG GAGAGCTGGG CCCATGGTCC
    TGGAAATTCC TCTTCAAGGG CCCTGACAGG GCATACCCAA CCGGAAAGGC AGCAATGGAA
    GATGGGCCAG CCAGCTCCCA AGATTTGGAA ACTACAACTC CAGGCCACCC TGCAAACCTG
    TCCCCCAGAC AGGAGGGGAA GTCATGGCCA CCCAGTGCCC CTGGGCTGGC CCATCCACCT
    TGTGAGGTTC CCAGCCAGAG CTGGCTATGG GAGTCTGAGT GTGCACAGAG AGAGAAAGAG
    GAGCCAGCGC TGGGCATCAC CAAAGTGCCT GATGCCTCAG GTGACAGGAG GCAGGACGTT
    CCATGCAAGA GCTGCCCCTT CACCCCAGAG CCTGGGCCCA GCCTGCTCCA GAAGGGCCAA
    GACCCAGGGG GCCCTCAGGA GCATCAGGAC TTGGCATTGT TACCTGATGA GCTTTTAAAG
    CAAACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18161
    Clone ID Related Accession (Same CDS sequence) XM_015462787.1 , XM_005204882.2
    Accession Version XM_005204882.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005204882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA
    TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC
    TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG
    ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG
    CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA
    ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG
    CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA
    ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC
    CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT
    CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG
    AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC
    CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG
    ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA
    GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA
    GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA
    ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC
    ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG
    ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC
    AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA
    GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC
    CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC
    CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT
    TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT
    GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA
    CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC
    ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC
    TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG
    GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA
    GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT
    CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT
    GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG
    GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC
    AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC
    AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG
    CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG
    TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC
    CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG
    CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG
    GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC
    CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC
    ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG
    GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC
    ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC
    ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC
    TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG
    TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG
    CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG
    GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG
    GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC
    ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG
    AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGAAAAA GGAGCTCCCT GTTCCGGAAG
    ATCACCAAGC AGGCATCCCT GCTCCACACC AGCCGCAGCC TTTCTTCCCT TAACCGCTCC
    CTGTCGTCCG GCGAGAGTGG GCCAGGCTCG CCCACACACA GCCACAGCCT TTCTCCCCGC
    TCTCCCACGC AGGGCTACCG GGTGACCCCT GAAGCCGTGC ACTCAGTGGG GGGGAATTCA
    TCACAGAGCA GCTCCCCCAG CTCCAGCGTG CCCAGTTCTC CTGCCGGCTC TGGGCACACA
    CGGCCGAGCT CCCTCCACGG TCTGGCGCCC AAGCTCCAAC GCCAGTACCG CTCTCCACGG
    CGCAAGTCAG CAGGCAGCAT CCCGCTGTCA CCACTGGCCC ACACCCCTTC ACCACCACCA
    GCGGCTTCGC CTCAGCGTTC CCCATCACCT CTGTCTGGCC ACGGGGCCCA GGCCTTCCCT
    ACCAAGCTCC ACTTGTCGCC TCCCCTAGGC CGGCAACTCT CACGGCCCAA GAGCGCGGAG
    CCACCCCGCT CACCACTACT TAAGAGGGTG CAGTCAGCTG AAAAACTGGC AGCTGCACTG
    GCTGCTTCTG AGAAGAAGCT CGCCACTTCT CGAAAACAGA GCCTTGACCT GCCCCACCCC
    GAGCTAAAGA AGGAATTGCC GCCCAGGGAA GTCAGCTCTC TGGAGGTAGT TGGGACCAGG
    AGTGTGCTGT CTGGAAAGGG GGCGCTGCCA GGGAAAGGGG TGTTGCAGGC TGCTCCCTCA
    CGGGCCTTAG GGACCCTCCG CCAGGACAGG GCTGAACGGC GAGAGTCGCT GCAGAAGCAG
    GAAGCCATCC GGGAGGTTGA CTCTTCAGAA GATGATACTG ATGAGGGGCC TGAGAACAGC
    CAAGGTGTTC AGGAGCCAAG CTTGGTACCC CACCGAGAAG GGGGCCAGGA TCCACCCCTC
    AGAGGAGCAG GAGAGGGTGA GGAAGAGGAT GCCTTCTTGC CCAGGGATCC CAGGGGACAG
    GGCCCAGTGG TCACAGGTCT ACTGACAGGG GTCACACTGG GATCTCCCAG AATGGAAGGC
    CCTGGTGTCC CCCAGAAGAA GCTTGGAATC CTACAGGCTT TTGAAGAGGG TTCCAGCTCC
    TCGTCCTCAG CCCCCAACCT GGGGAAGGCT GGCCCCACAG ACCCCATCCC CGCTGAAGGC
    TGCTGGAAGG CCCAGCACCT CCACACCCAG GCACTGACAG CACTTTGTCC GAGCTCATCA
    GGACGCATCC CTGCCAGTTG CTCCACTGCC TCCACCCCTG GAGAGCTGGG CCCATGGTCC
    TGGAAATTCC TCTTCAAGGG CCCTGACAGG GCATACCCAA CCGGAAAGGC AGCAATGGAA
    GATGGGCCAG CCAGCTCCCA AGATTTGGAA ACTACAACTC CAGGCCACCC TGCAAACCTG
    TCCCCCAGAC AGGAGGGGAA GTCATGGCCA CCCAGTGCCC CTGGGCTGGC CCATCCACCT
    TGTGAGGTTC CCAGCCAGAG CTGGCTATGG GAGTCTGAGT GTGCACAGAG AGAGAAAGAG
    GAGCCAGCGC TGGGCATCAC CAAAGTGCCT GATGCCTCAG GTGACAGGAG GCAGGACGTT
    CCATGCAAGA GCTGCCCCTT CACCCCAGAG CCTGGGCCCA GCCTGCTCCA GAAGGGCCAA
    GACCCAGGGG GCCCTCAGGA GCATCAGGAC TTGGCATTGT TACCTGATGA GCTTTTAAAG
    CAAACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005204939.1
    CDS299..5707
    Translation

    Target ORF information:

    RefSeq Version XM_005204882.2
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204882.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAGCGGA GCCGCTGCCG CGATCGACCG CAGCCGCCGC CGCCGCCGCT GCCGCAGACA 
    GCCGGCCGGG AGGATGGGGC TCAGCGGGCG GCGGAGCTGC CCCAGCCCCA GCCCCTGCCC
    CCGCGCCGGA GAGCGCCGCC CGGGAGGCAG CGACTGGAGG AGCGGACCGG GCCAGTAGGG
    CCCGAAGTCA GGGAGCAGGA TGTGGTAACT GGACGTAGTC CGCTGCTCTT CAGGAAGCTC
    AGTAATCCTG ACATATTTTC ATCCACTGGA AAAGCTAAAC TTCAACGACA ACTTAGTCAG
    GATGACTGTA AGTTACGGAG AGGAAGCCTG GCCAGTTCTT TGTCAGGCAA GCAGCTGCTC
    CCCTTGTCCG GCAGTGCACA CAGCAGCGTG GGACAGGTGA CTTGGCAGTC TTCAGGAGAA
    GCGTCCAACC TGGTGCGGAT GAGGAATCAG TCCCTCGGAC AGTCTGCGCC TTCTCTTACC
    GCTGGCTTGA AGGAGCTGAG CCTTCCAAGA AGAGGCAGCT TCTGTCGGAC AAGTAACCGC
    AAGAGTTTGA TTGTGACCTC TAGTACGTCA CCTACACTGC CACGGCCACA CTCACCACTC
    CATGGCCACA CAGGTAACAG TCCTTTGGAT AGTCCCCGGA ATTTCTCTCC AAATGCACCT
    GCTCACTTTT CCTTTGTTCC TGCCCGTAGG ACTGATGGAC GGCGCTGGTC TTTGGCCTCA
    TTGCCTTCTT CAGGCTATGG AACCAACACC CCTAGTTCTA CCGTGTCATC GTCGTGCTCC
    TCTCAGGAGA AGCTGCACCA GTTGCCTTTC CAGCCCACGG CCGATGAGCT GCACTTCTTG
    ACGAAGCATT TCAGCACAGA CAGTGTGCCT GATGAGGAGG GGCGGCAGTC CCCCGCCCTG
    CGGCCCCGCT CCCGCAGCCT CAGTCCTGGA CGGTCACCAG TTTCCTTTGA CAGTGAAATA
    ATAATGATGA ATCATGTATA CAAGGAAAGA TTCCCCAAGG CCACTGCACA AATGGAAGAG
    CGTCTGGCCG AGTTCATCTC CTCCAATACT CCAGACAACG TGTTGCCCTT GGCTGATGGA
    ACCTTGAGCT TTATTCATCA TCAGGTGATA GAGATGGCCC GAGACTGCCT GGACAAATCC
    CGGAGTGGCC TCATTACCTC ACACTACTTC TACGAACTTC AAGAGAATTT AGAGAAGCTT
    CTGCAAGATG CTCATGAGCG CTCAGAGAGT TCAGAAGTGG CTTTTGTGAT GCAGCTGGTG
    AAAAAGCTGA TGATTGTCAT TGCCCGCCCA GCTCGTCTCC TGGAATGCCT GGAGTTTGAC
    CCTGAAGAGT TCTACCACCT GTTAGAAGCA GCTGAGGGCC ACGCCAAAGA GGGACAAGGG
    ATTAAATGTG ACATTCCCCG CTACATCGTT AGCCAACTGG GCCTCACACG GGATCCCCTA
    GAGGAAATGG CCCAGTTGAG CAGCTATGAC AGTCCTGATA CTCCGGAGAC AGATGATTCA
    GTCGAGGGCC GTGGGGCATC TCTGACATCG AAAAAGACAC CCTCTGAGGA GGACTTCGAA
    ACCATTAAGC TCATCAGCAA TGGCGCCTAT GGGGCCGTGT TCCTGGTGCG GCACAAGTCC
    ACCCGGCAGC GCTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT GCGGAACCAG
    ATCCAGCAGG CCTTCGTGGA GCGGGACATC CTGACTTTTG CTGAGAACCC CTTTGTGGTC
    AGCATGTTCT GCTCCTTTGA AACCAAGCGC CACCTGTGCA TGGTGATGGA GTATGTGGAA
    GGAGGAGACT GTGCCACGCT GCTGAAGAAC ATCGGGGCCC TGCCTGTGGA CATGGTGCGC
    CTGTACTTTG CAGAAACTGT GCTAGCCCTG GAGTACTTAC ACAATTACGG CATCGTGCAC
    CGTGACCTCA AGCCTGACAA CCTGCTGATT ACATCCATGG GTCACATCAA GCTCACTGAT
    TTTGGCCTTT CCAAAATTGG CCTTATGAGT CTGACCACAA ACTTGTACGA GGGCCACATT
    GAAAAGGATG CACGGGAGTT CCTGGACAAG CAGGTATGCG GGACCCCGGA GTACATTGCA
    CCTGAGGTGA TCCTGCGGCA GGGCTACGGG AAGCCAGTGG ACTGGTGGGC TATGGGCATC
    ATCCTGTACG AGTTCCTGGT GGGCTGCGTC CCTTTCTTTG GAGACACTCC GGAGGAGCTC
    TTTGGGCAAG TGATCAGTGA TGAGATTGTG TGGCCTGAGG GGGACGATGC ACTGCCGCCG
    GACGCCCAAG ATCTCACCTC AAAACTGCTC CACCAGAACC CATTGGAGAG ACTGGGCACA
    GGCAGTGCCT ATGAGGTGAA GCAGCACCCA TTCTTCACTG GTCTGGACTG GACGGGACTT
    CTTCGCCAGA AGGCTGAATT TATTCCTCAG CTGGAGTCAG AGGATGATAC CAGCTACTTT
    GACACCCGCT CAGAGCGATA CCACCACGTG GACTCGGAGG ATGAGGAGGA AGTGAGTGAG
    GATGGCTGCC TTGAGATTCG CCAGTTCTCG TCCTGTTCTC CAAGGTTCAG CAAGGTGTAC
    AGCAGCATGG AGCGGCTCTC GCTGCTCGAG GAGCGCCGGA CGCCACCCCC CACCAAGCGC
    AGCCTGAGTG AGGAAAAGGA GGACCGCACG GATGGCCTGG CGGGGCTGAG GGGCCGAGAG
    CGAAGCTGGG TGATCGGCTC CCCTGAGATA TTACGGAAGC GGCTGTCGGT GTCCGAGTCG
    TCCCACACGG AAAGCGATTC CAGCCCTCCA CTCACCGTGC GGCGCCACTG CTCGGGCCTC
    CTGGACGTGC CTCGCTTCCC TGAGAGCACT GAGGAGGCTG TTGGCGCTCC CAGGAGGCAG
    CAGCAGGAGG GTGCATGGCT TCTGACCCCG TCTGGAGAGG GGGCGTCTGG GCCTGCCCTG
    GAACGGCCGG CCGAGCGGCG GCTGAAGCTG AATGAGGAAC CTGCTGGCCA GAGCAGCGCC
    CCCAGCCCAG CCATGGAGAC CCGAGGAGGC CGCGGAACCC CACAGCTGGC AGAAGGAGCC
    ACAGCCAGGG CCATCAGCGA CCTGGCGGTG CGCAGGGCCC GCCACCGGCT GCTCTCTGGG
    GACTCCATAG AAAAGCGCAC CGCTCGCCCC ATCAACAAAG TGATCAAGTC CGCCTCAGCC
    ACAGCCCTGT CCCTTCTCAT TCCTGCTGAG CACCACTCCT GCTCTCCACT GGCCAGCCCC
    ATGTCCCCAC ATTCCCAGTC ATCCAACCCA TCATCCAGGG ACTCTTCTCC AAGCAGGGAC
    TTCCTGCCAG CCCTCAGCAG CTCACGGCCC CCCATTGTCA TCCACCGAGC CGGCAAGAAG
    TATGGCTTCA CCCTACGAGC CATCCGTGTC TACATGGGTG ACTCAGATGT CTACACCGTG
    CACCACATGG TGTGGCACGT GGAGGACGGA GGTCCAGCCA GTGAGGCAGG GCTGCGTCAG
    GGGGACCTCA TCACGCACGT CAACGGGGAG CCTGTGCACG GGCTGGTGCA CACGGAGGTG
    GTGGAGCTGA TCCTGAAGAG TGGAAACAAG GTGTCTATTT CAACAACTCC CCTGGAGAAC
    ACCTCCATTA AGGTGGGGCC CGCTCGGAAG GGCAGCTACA AGGCCAAGAT GGCCCGAAGG
    AGCAAGCGGA GCCGGGGCAA GGATGGACAA GAAAGAAAAA GGAGCTCCCT GTTCCGGAAG
    ATCACCAAGC AGGCATCCCT GCTCCACACC AGCCGCAGCC TTTCTTCCCT TAACCGCTCC
    CTGTCGTCCG GCGAGAGTGG GCCAGGCTCG CCCACACACA GCCACAGCCT TTCTCCCCGC
    TCTCCCACGC AGGGCTACCG GGTGACCCCT GAAGCCGTGC ACTCAGTGGG GGGGAATTCA
    TCACAGAGCA GCTCCCCCAG CTCCAGCGTG CCCAGTTCTC CTGCCGGCTC TGGGCACACA
    CGGCCGAGCT CCCTCCACGG TCTGGCGCCC AAGCTCCAAC GCCAGTACCG CTCTCCACGG
    CGCAAGTCAG CAGGCAGCAT CCCGCTGTCA CCACTGGCCC ACACCCCTTC ACCACCACCA
    GCGGCTTCGC CTCAGCGTTC CCCATCACCT CTGTCTGGCC ACGGGGCCCA GGCCTTCCCT
    ACCAAGCTCC ACTTGTCGCC TCCCCTAGGC CGGCAACTCT CACGGCCCAA GAGCGCGGAG
    CCACCCCGCT CACCACTACT TAAGAGGGTG CAGTCAGCTG AAAAACTGGC AGCTGCACTG
    GCTGCTTCTG AGAAGAAGCT CGCCACTTCT CGAAAACAGA GCCTTGACCT GCCCCACCCC
    GAGCTAAAGA AGGAATTGCC GCCCAGGGAA GTCAGCTCTC TGGAGGTAGT TGGGACCAGG
    AGTGTGCTGT CTGGAAAGGG GGCGCTGCCA GGGAAAGGGG TGTTGCAGGC TGCTCCCTCA
    CGGGCCTTAG GGACCCTCCG CCAGGACAGG GCTGAACGGC GAGAGTCGCT GCAGAAGCAG
    GAAGCCATCC GGGAGGTTGA CTCTTCAGAA GATGATACTG ATGAGGGGCC TGAGAACAGC
    CAAGGTGTTC AGGAGCCAAG CTTGGTACCC CACCGAGAAG GGGGCCAGGA TCCACCCCTC
    AGAGGAGCAG GAGAGGGTGA GGAAGAGGAT GCCTTCTTGC CCAGGGATCC CAGGGGACAG
    GGCCCAGTGG TCACAGGTCT ACTGACAGGG GTCACACTGG GATCTCCCAG AATGGAAGGC
    CCTGGTGTCC CCCAGAAGAA GCTTGGAATC CTACAGGCTT TTGAAGAGGG TTCCAGCTCC
    TCGTCCTCAG CCCCCAACCT GGGGAAGGCT GGCCCCACAG ACCCCATCCC CGCTGAAGGC
    TGCTGGAAGG CCCAGCACCT CCACACCCAG GCACTGACAG CACTTTGTCC GAGCTCATCA
    GGACGCATCC CTGCCAGTTG CTCCACTGCC TCCACCCCTG GAGAGCTGGG CCCATGGTCC
    TGGAAATTCC TCTTCAAGGG CCCTGACAGG GCATACCCAA CCGGAAAGGC AGCAATGGAA
    GATGGGCCAG CCAGCTCCCA AGATTTGGAA ACTACAACTC CAGGCCACCC TGCAAACCTG
    TCCCCCAGAC AGGAGGGGAA GTCATGGCCA CCCAGTGCCC CTGGGCTGGC CCATCCACCT
    TGTGAGGTTC CCAGCCAGAG CTGGCTATGG GAGTCTGAGT GTGCACAGAG AGAGAAAGAG
    GAGCCAGCGC TGGGCATCAC CAAAGTGCCT GATGCCTCAG GTGACAGGAG GCAGGACGTT
    CCATGCAAGA GCTGCCCCTT CACCCCAGAG CCTGGGCCCA GCCTGCTCCA GAAGGGCCAA
    GACCCAGGGG GCCCTCAGGA GCATCAGGAC TTGGCATTGT TACCTGATGA GCTTTTAAAG
    CAAACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.