mast2 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021474106.1	XP_021329781.1	microtubule-associated serine/threonine-protein kinase 2 isoform X8
XM_021474609.1	XP_021330284.1	microtubule-associated serine/threonine-protein kinase 2 isoform X2
XM_009302494.3	XP_009300769.1	microtubule-associated serine/threonine-protein kinase 2 isoform X4
XM_021471802.1	XP_021327477.1	microtubule-associated serine/threonine-protein kinase 2 isoform X3
XM_001341269.7	XP_001341305.4	microtubule-associated serine/threonine-protein kinase 2 isoform X3
XM_021471799.1	XP_021327474.1	microtubule-associated serine/threonine-protein kinase 2 isoform X7
XM_009302496.2	XP_009300771.1	microtubule-associated serine/threonine-protein kinase 2 isoform X5
XM_009302495.2	XP_009300770.1	microtubule-associated serine/threonine-protein kinase 2 isoform X2
XM_005166291.4	XP_005166348.1	microtubule-associated serine/threonine-protein kinase 2 isoform X1
XM_009302496.3	XP_009300771.1	microtubule-associated serine/threonine-protein kinase 2 isoform X2
XM_021477182.1	XP_021332857.1	microtubule-associated serine/threonine-protein kinase 2 isoform X3
XM_021471800.1	XP_021327475.1	microtubule-associated serine/threonine-protein kinase 2 isoform X1
XM_021471801.1	XP_021327476.1	microtubule-associated serine/threonine-protein kinase 2 isoform X2
XM_021474607.1	XP_021330282.1	microtubule-associated serine/threonine-protein kinase 2 isoform X11
XM_021474605.1	XP_021330280.1	microtubule-associated serine/threonine-protein kinase 2 isoform X9
XM_021474606.1	XP_021330281.1	microtubule-associated serine/threonine-protein kinase 2 isoform X10
XM_021471798.1	XP_021327473.1	microtubule-associated serine/threonine-protein kinase 2 isoform X6
XM_009302494.2	XP_009300769.1	microtubule-associated serine/threonine-protein kinase 2 isoform X1
XM_005166291.3	XP_005166348.1	microtubule-associated serine/threonine-protein kinase 2 isoform X4
XM_001341269.8	XP_001341305.4	microtubule-associated serine/threonine-protein kinase 2 isoform X5
XM_021474608.1	XP_021330283.1	microtubule-associated serine/threonine-protein kinase 2 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa143092	XM_021477182.1 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa144726	XM_021471798.1 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144727	XM_021471799.1 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144728	XM_021471800.1 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144729	XM_021471801.1 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144730	XM_021471802.1 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa146864	XM_021474605.1 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa146865	XM_021474606.1 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa146866	XM_021474607.1 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa146867	XM_021474608.1 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa146868	XM_021474609.1 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa11957	XM_001341269.8 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11958	XM_005166291.4 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47560	XM_009302494.3 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47561	XM_009302495.2 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47562	XM_009302496.3 Latest version!	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa47562	XM_009302496.2	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47560	XM_009302494.2	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11958	XM_005166291.3	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11957	XM_001341269.7	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

mast2 ( XM_021477182.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332857.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa143092
Clone ID Related Accession (Same CDS sequence)	XM_021477182.1
Accession Version	XM_021477182.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACAG GTGGGCCAGA CGGGGATCGC GGTATAAATC GCATCTTCAG AAGGTGCTCA 
GAATTGCATT CCAGACTTCT CCATTTAACA CCTTCATCGC TCGCGCTGAG GATGTCCTGC 
GTCTCATGGC AGAGGCGGCT GGAGGAAGAG GAGGTTCTTC AACAGATTTT AGAGCGCTGG 
GAGCAGAGCG CACTCAGAAA CCATGTGGAT CTAACAGCTC ATGAGCGTTC AGAAAGTGGG 
GAGGTGACAT TTGTCACCCA GCTAGTTAAA AAGCTGATGA TAATCATCGC TCGGCCTGCC 
CGGCTGCTAG AATGTTTGGA GTTTGACCCG GAGGAGTTCT ACCACCTGTT AGAGGCAGCT 
GAAGGTCATG CTAAAGAAGG TCAAGGCATC AAGTCTGACA TCCCTCGATA CATCATCAGC 
CAGCTGGGCC TCACCAGGGA CCCCCTTGAG GAAATGGCCC AGCTGAGCAG CTATGACAGC 
GGTAACCCTG AAACACCAGA GACAGAGACA GATTCCACAG ATAGTCGAGG AGCAACCGTA 
CAGCCACTCC AACCTCAGCC TCAACCGCAG CCCAAGACTA AAACACCTCG CGAGGAAGAC 
TTCGAGACCA TTAAACTCAT CAGTAATGGA GCCTATGGGG CTGTGTTTCT GGTGAGGCAT 
AAAGAGACCC GTCAGCGATT TGCCATGAAG AAGATCAACA AGCAGAACCT GATCCTAAGA 
AACCAGATCC AGCAGGCCTT TGTGGAAAGA GACATCTTGA CATTTGCAGA AAACCCTTTT 
GTTGTCAGCA TGTTCTGCTC CTTTGAGACA AGGAGACATC TTTGCATGGT TATGGAGTAT 
GTGGAGGGTG GGGATTGTGC CACTCTCCTG AAGCACATTG GAGCTCTGCC TGTGGATATG 
GCACGCATGT ACTTTGCTGA AACCGTTCTT GCATTGGAAT ACCTTCACAA CTATGGTATT 
GTGCACAGAG ACCTCAAACC TGACAATCTC CTGATTACCT CAATGGGACA CGTCAAACTG 
ACTGACTTTG GCCTGTCCAA GATTGGTTTA ATGAGCTTAA CAACTAATCT GTATGAGGGC 
CACATTGAAA AAGATACTCG AGAATTCTTG GACAAACAGG TGTGTGGTAC TCCTGAATAC 
ATTGCTCCTG AGGTTATTCT GCGACAGGGT TATGGAAAAC CAGTTGACTG GTGGGCCATG 
GGAGTCATCT TGTATGAGTT TCTGGTGGGC TGTGCTCCTT TCTTTGGAGA TACACCTGAA 
GAACTGTTTG GACAAGTCAT CAGTGATGAG ATAATCTGGC CAGAAGGTGA TGAAGCTTTG 
CCTCCGGATG CTCAAGATCT CATCTCCAAA CTTCTTCGGC AAAACCCTCT AGAGCGTCTT 
GGGACAGGGA GTGCGTTTGA GGTAAAACAG CACCGCTTCT TTACTGATCT GGACTGGAAC 
AGCCTCCTGA GGCAGAAGGC TGAGTTCATT CCTCAGCTGG AGTCGGAGGA TGACACTAGC 
TACTTTGACA CACGCTCAGA TCGTTATCAT CATGTGGACT CAGAGGAAGA AGATGACACA 
AATGATGACG ACCATTTGGA GATCCGTCAG TTCTCCTCCT GTTCGCCACG ATTCAGCAAG 
GTGTATAGTA GTATGGAGCG TCTTTCCTTG CATGAGGAGA AACGCACCCC TCCTCCTACT 
AAGCGCAGTC TGAGTGAAGA GGGAGGAGAG CGGATCGACA GTCTAAGTGG CCTAAAGTCC 
AGAGACCGAA GCTGGTTGGT GGGCTCACCT GAAATTCTCA GAAAGCGTCT CTCTGTTTCC 
GAGTCTTCTC ACACCGAGAG TGACTCGAGT CCACCTCTGA CTGTTCGTCG CCGCTGCTGC 
TCGGCCATCA TTGAAATGCC TCGCTTCGCC ATATCCAGTG AAGAGGATAG CTGTGTGGGG 
AGCAGGAAGA CCCCTGTGAG AGGCCCTCGA GGGGATGATC TGCCTCAGGC CATACCAGAG 
CTCCCTGTAG AGAGAGAGCT CAGGCTGGAT GAATCGCCCA CCACGCCAGG ATCCACTTCC 
AGTCAAATCA GCCAGTCCAC ACTCACACCT GGTAGTTCTG GTGATGTGTT AGATCGTGCT 
TCTCGCTATA GTGCTGAAGT TTCTGAAAAC TCCACTCCAA GAGCCATCAG TGATTTGGCG 
GCTCGAAGAG CTCGCCACAG ACTGCTTTCA GGAGATACAG ATAAACACAC ATCACGTCCA 
CTTAGCAAAG TCATCAAATC AGCTTCAGCA ACAACCCTGT CACTCATGAT CCCTGCTGAT 
CACCATGGTG CTTCTCCACT GGCCAGTCCC ATGTCGCCTC ACTCTCTCTC TTCGAACCCC 
TCATCACGTG ACTCCTCGCC CAGCCGTGAC CTTTCCCCAG CTGTCTGTAG TGTTAAACCT 
GCCATAATCA TCCATCGAGC TGGAAAGAAA TATGGTTTTA CTTTACGTGC AATCCGGGTT 
TACATGGGAG ACACAGACAT ATATACTGTC CATCACATGG TCTGGCATGT GGAGGATGGA 
GGACCTGCTC ATGAGGCTGG ACTGAGAGAG GGAGATCTCA TCACACATGT TAACGGAGAG 
CCTGTCCATG GACTTGTGCA CACAGAAGTT GTTGAGCTCA TTCTTAAGAG TGGCAGTAAG 
GTGTCCATCT CTGCTACACC CTTTGAAAAT ACATCAATTA AGGTTGGTCC TGCTCGCAAA 
ACTAGCTACA AGTCCAAAAT GGCCCGTCGC AACAAAAGAA CCAAAACTAG AGAAGGACAA 
GACAGTAAGA AGAGGAATTC CCTCTTCCGT AAGATCACCA AACAGGCGAC CCTTCTTCAC 
ACCAGTCGCA GCCTGTCCTC TCTGAATCGC TCGGTCTCTT CTGGTGAGAG CGTCCCAGGC 
TCTCCTACAC ACATGTCACC TCGTTCACCC ACACAGGGCT GTCGTTCAAC ACCTGACTCC 
GCCCACTCAG TTGGTGGAAA CTCATCCCAA AGTAGCTCGC CTAGCTCCAG TGTGCCAAAC 
TCTCCTGCCA GCTCTGGCCA GATCAGACCC AGCTCCTTGC ATGGTCTGGC CCCCAAGCTG 
CAGCGCCAGT ACCGATCCCC CCGCCGGAAG TCTGCAGGCA ACATCCCTTT ATCCCCTCTG 
GCCCGTACAC CATCGCCCAC TCCACAGAGC ACTTCTCCCC AGCGTTCCCC TTCTCCCCTG 
CCAAGCCACG GGCTCATCAC TTCTTCAATA GGCCAGTCTT TTCCTGTCAA ACTGCACTCT 
TCACCTCCAT TAGTAAGACA GATATCTCGG CCAAAGAGTG CAGACCCTCC TCGCTCTCCT 
CTGCTCAAAC GAGTCCAGTC CGCAGAGAAA CTCGCCGCCT CTCTTTCTTC TTCATCCTCC 
TCTCCTTCTC CCTCTTCGGC TGCTGATAAA AAGCTTCCAG TTGGTGCCAG CCGCAAACAC 
AGTCTTGATA CCTCACATTC AGAGTTTAAA AAAGAGATGC TGCAGCGAGA CCCTAGTCTA 
CAGAGCCTGC AAGAGTCAGC CAGTGAGACT CTTATGGGAG GAAGAGTGTC TCCTGCAGAA 
AAGGGAAGCC TGCAGAAAAG TTCAGTGCGT AAACTGGGCA GGCAAGAAGG TCCCGAGTCT 
GGAACAGGAA CTCTGGGACT TGTTCCTGGA AAGAGTAAAT TGAAAGACAA GCTGTCTGCC 
ATACGTGCTG AACGCCGCGA ATCTCTTCAA AAGCAGGATG CCATCCATGA AGTGGATTCA 
TCTGAAGATG AGACAGACGA AGGGTCGGAA GACAGCCAAG ATGGGCGCAG AACTTGCTAT 
GCTCCTCCTA GCCATGTCTT GAGGCCTACA ACTGTAATGG CCTCTCCCCA TACCATCAGA 
ATGGGGCCAG CGGCTCCACC AACACTGGGA CTTTTTCCTT CAACAGTTGC ACCGCCATGC 
TCACCTCAGC TCATCAGAGG ACTGCAGATA CAAAACCAAA GTACCACTCA ATCTCAGACA 
CAAACTTCAA CCTCACAACC AGCGCAAGCT GCAGTTAAAC CCCCAGAACA GAAACCAATA 
ACCTCTGTTA AAGACTCAAG TTCAACGATA ATTGATGATT CGAAAAAACA GTCACAGGCA 
CAAGACATTC AGTCCAGACC TTCTCCACAT CAACCCAGTC CTCTGTCTAG TACCTTTTCC 
ACCCAAGGCA GCGCCTTTAC CTCTGTTAGT AAAGACCCAT TTGTCAAGCC CACCACACCT 
CCATTGGATC CCCGGAGGAC CCCAAAAATG TCTGAGCCAA GGTCAAAAAC CAGTGGTAGA 
ACTGATTCTA GCACTACTTC TGGAGTAGCC AGGGATACCC CAGTTGCGAC TACCCCAACA 
GGAGGAATCA CTAAGGAGAT GAAGGAGGCT GACAATCGCA GACAGGCTGC AGCTGCTGCT 
GCTGCCTCTG CCGCCGCTGC TACCACCTCT GCATCCACTG CCTCTGAAAG TGGAATGGAT 
AAAGTTGTGT CTCAGCTCGC CACTGTGGCC AAGAGTGTGC TTGGACCTGT CAAGCTCAAT 
ATCCCAGGTA CTAAAGAGAC CATGGAACGA ACAAAGGACC AGCGTACCCA GGCAGATGCC 
ACCCACCCAA AGATAAAAGA ATGTCGCCTG GAGCACCCTG ATTCTCCTGA CCAATTGAGT 
CCATCTGCTG CTTCGGGCTC CCAGTCTCCA CGACTGACTG AATCTCCATC TAAACAAACA 
TCATCCAAAT CTGAGCCAGC CTCAACTTCT CAGAGACCTT TAGAGGTCCC AGGGACTTTC 
AAAAGACAAA CTTCTCCAAT CCCTCAAGCT AGGGAAGAGC ATCAGGATAG GTCAGGAACT 
CTGCAAAGTG GCACCACATC AAGGCCTAGG TCTGAAGCAC AATCACAGAC CCAGCAGGCA 
AAGCCCAGTT CCACTACGTC ACGAGATAAA TCCAACAAGA CAACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332857.1
CDS	110..5017
Translation	MVTGGPDGDRGINRIFRRCSELHSRLLHLTPSSLALRMSCVSWQRRLEEEEVLQQILERWEQSALRNHVDLTAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYAPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPCSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_021477182.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477182.1

ORF Insert Sequence:

ATGGTGACAG GTGGGCCAGA CGGGGATCGC GGTATAAATC GCATCTTCAG AAGGTGCTCA 
GAATTGCATT CCAGACTTCT CCATTTAACA CCTTCATCGC TCGCGCTGAG GATGTCCTGC 
GTCTCATGGC AGAGGCGGCT GGAGGAAGAG GAGGTTCTTC AACAGATTTT AGAGCGCTGG 
GAGCAGAGCG CACTCAGAAA CCATGTGGAT CTAACAGCTC ATGAGCGTTC AGAAAGTGGG 
GAGGTGACAT TTGTCACCCA GCTAGTTAAA AAGCTGATGA TAATCATCGC TCGGCCTGCC 
CGGCTGCTAG AATGTTTGGA GTTTGACCCG GAGGAGTTCT ACCACCTGTT AGAGGCAGCT 
GAAGGTCATG CTAAAGAAGG TCAAGGCATC AAGTCTGACA TCCCTCGATA CATCATCAGC 
CAGCTGGGCC TCACCAGGGA CCCCCTTGAG GAAATGGCCC AGCTGAGCAG CTATGACAGC 
GGTAACCCTG AAACACCAGA GACAGAGACA GATTCCACAG ATAGTCGAGG AGCAACCGTA 
CAGCCACTCC AACCTCAGCC TCAACCGCAG CCCAAGACTA AAACACCTCG CGAGGAAGAC 
TTCGAGACCA TTAAACTCAT CAGTAATGGA GCCTATGGGG CTGTGTTTCT GGTGAGGCAT 
AAAGAGACCC GTCAGCGATT TGCCATGAAG AAGATCAACA AGCAGAACCT GATCCTAAGA 
AACCAGATCC AGCAGGCCTT TGTGGAAAGA GACATCTTGA CATTTGCAGA AAACCCTTTT 
GTTGTCAGCA TGTTCTGCTC CTTTGAGACA AGGAGACATC TTTGCATGGT TATGGAGTAT 
GTGGAGGGTG GGGATTGTGC CACTCTCCTG AAGCACATTG GAGCTCTGCC TGTGGATATG 
GCACGCATGT ACTTTGCTGA AACCGTTCTT GCATTGGAAT ACCTTCACAA CTATGGTATT 
GTGCACAGAG ACCTCAAACC TGACAATCTC CTGATTACCT CAATGGGACA CGTCAAACTG 
ACTGACTTTG GCCTGTCCAA GATTGGTTTA ATGAGCTTAA CAACTAATCT GTATGAGGGC 
CACATTGAAA AAGATACTCG AGAATTCTTG GACAAACAGG TGTGTGGTAC TCCTGAATAC 
ATTGCTCCTG AGGTTATTCT GCGACAGGGT TATGGAAAAC CAGTTGACTG GTGGGCCATG 
GGAGTCATCT TGTATGAGTT TCTGGTGGGC TGTGCTCCTT TCTTTGGAGA TACACCTGAA 
GAACTGTTTG GACAAGTCAT CAGTGATGAG ATAATCTGGC CAGAAGGTGA TGAAGCTTTG 
CCTCCGGATG CTCAAGATCT CATCTCCAAA CTTCTTCGGC AAAACCCTCT AGAGCGTCTT 
GGGACAGGGA GTGCGTTTGA GGTAAAACAG CACCGCTTCT TTACTGATCT GGACTGGAAC 
AGCCTCCTGA GGCAGAAGGC TGAGTTCATT CCTCAGCTGG AGTCGGAGGA TGACACTAGC 
TACTTTGACA CACGCTCAGA TCGTTATCAT CATGTGGACT CAGAGGAAGA AGATGACACA 
AATGATGACG ACCATTTGGA GATCCGTCAG TTCTCCTCCT GTTCGCCACG ATTCAGCAAG 
GTGTATAGTA GTATGGAGCG TCTTTCCTTG CATGAGGAGA AACGCACCCC TCCTCCTACT 
AAGCGCAGTC TGAGTGAAGA GGGAGGAGAG CGGATCGACA GTCTAAGTGG CCTAAAGTCC 
AGAGACCGAA GCTGGTTGGT GGGCTCACCT GAAATTCTCA GAAAGCGTCT CTCTGTTTCC 
GAGTCTTCTC ACACCGAGAG TGACTCGAGT CCACCTCTGA CTGTTCGTCG CCGCTGCTGC 
TCGGCCATCA TTGAAATGCC TCGCTTCGCC ATATCCAGTG AAGAGGATAG CTGTGTGGGG 
AGCAGGAAGA CCCCTGTGAG AGGCCCTCGA GGGGATGATC TGCCTCAGGC CATACCAGAG 
CTCCCTGTAG AGAGAGAGCT CAGGCTGGAT GAATCGCCCA CCACGCCAGG ATCCACTTCC 
AGTCAAATCA GCCAGTCCAC ACTCACACCT GGTAGTTCTG GTGATGTGTT AGATCGTGCT 
TCTCGCTATA GTGCTGAAGT TTCTGAAAAC TCCACTCCAA GAGCCATCAG TGATTTGGCG 
GCTCGAAGAG CTCGCCACAG ACTGCTTTCA GGAGATACAG ATAAACACAC ATCACGTCCA 
CTTAGCAAAG TCATCAAATC AGCTTCAGCA ACAACCCTGT CACTCATGAT CCCTGCTGAT 
CACCATGGTG CTTCTCCACT GGCCAGTCCC ATGTCGCCTC ACTCTCTCTC TTCGAACCCC 
TCATCACGTG ACTCCTCGCC CAGCCGTGAC CTTTCCCCAG CTGTCTGTAG TGTTAAACCT 
GCCATAATCA TCCATCGAGC TGGAAAGAAA TATGGTTTTA CTTTACGTGC AATCCGGGTT 
TACATGGGAG ACACAGACAT ATATACTGTC CATCACATGG TCTGGCATGT GGAGGATGGA 
GGACCTGCTC ATGAGGCTGG ACTGAGAGAG GGAGATCTCA TCACACATGT TAACGGAGAG 
CCTGTCCATG GACTTGTGCA CACAGAAGTT GTTGAGCTCA TTCTTAAGAG TGGCAGTAAG 
GTGTCCATCT CTGCTACACC CTTTGAAAAT ACATCAATTA AGGTTGGTCC TGCTCGCAAA 
ACTAGCTACA AGTCCAAAAT GGCCCGTCGC AACAAAAGAA CCAAAACTAG AGAAGGACAA 
GACAGTAAGA AGAGGAATTC CCTCTTCCGT AAGATCACCA AACAGGCGAC CCTTCTTCAC 
ACCAGTCGCA GCCTGTCCTC TCTGAATCGC TCGGTCTCTT CTGGTGAGAG CGTCCCAGGC 
TCTCCTACAC ACATGTCACC TCGTTCACCC ACACAGGGCT GTCGTTCAAC ACCTGACTCC 
GCCCACTCAG TTGGTGGAAA CTCATCCCAA AGTAGCTCGC CTAGCTCCAG TGTGCCAAAC 
TCTCCTGCCA GCTCTGGCCA GATCAGACCC AGCTCCTTGC ATGGTCTGGC CCCCAAGCTG 
CAGCGCCAGT ACCGATCCCC CCGCCGGAAG TCTGCAGGCA ACATCCCTTT ATCCCCTCTG 
GCCCGTACAC CATCGCCCAC TCCACAGAGC ACTTCTCCCC AGCGTTCCCC TTCTCCCCTG 
CCAAGCCACG GGCTCATCAC TTCTTCAATA GGCCAGTCTT TTCCTGTCAA ACTGCACTCT 
TCACCTCCAT TAGTAAGACA GATATCTCGG CCAAAGAGTG CAGACCCTCC TCGCTCTCCT 
CTGCTCAAAC GAGTCCAGTC CGCAGAGAAA CTCGCCGCCT CTCTTTCTTC TTCATCCTCC 
TCTCCTTCTC CCTCTTCGGC TGCTGATAAA AAGCTTCCAG TTGGTGCCAG CCGCAAACAC 
AGTCTTGATA CCTCACATTC AGAGTTTAAA AAAGAGATGC TGCAGCGAGA CCCTAGTCTA 
CAGAGCCTGC AAGAGTCAGC CAGTGAGACT CTTATGGGAG GAAGAGTGTC TCCTGCAGAA 
AAGGGAAGCC TGCAGAAAAG TTCAGTGCGT AAACTGGGCA GGCAAGAAGG TCCCGAGTCT 
GGAACAGGAA CTCTGGGACT TGTTCCTGGA AAGAGTAAAT TGAAAGACAA GCTGTCTGCC 
ATACGTGCTG AACGCCGCGA ATCTCTTCAA AAGCAGGATG CCATCCATGA AGTGGATTCA 
TCTGAAGATG AGACAGACGA AGGGTCGGAA GACAGCCAAG ATGGGCGCAG AACTTGCTAT 
GCTCCTCCTA GCCATGTCTT GAGGCCTACA ACTGTAATGG CCTCTCCCCA TACCATCAGA 
ATGGGGCCAG CGGCTCCACC AACACTGGGA CTTTTTCCTT CAACAGTTGC ACCGCCATGC 
TCACCTCAGC TCATCAGAGG ACTGCAGATA CAAAACCAAA GTACCACTCA ATCTCAGACA 
CAAACTTCAA CCTCACAACC AGCGCAAGCT GCAGTTAAAC CCCCAGAACA GAAACCAATA 
ACCTCTGTTA AAGACTCAAG TTCAACGATA ATTGATGATT CGAAAAAACA GTCACAGGCA 
CAAGACATTC AGTCCAGACC TTCTCCACAT CAACCCAGTC CTCTGTCTAG TACCTTTTCC 
ACCCAAGGCA GCGCCTTTAC CTCTGTTAGT AAAGACCCAT TTGTCAAGCC CACCACACCT 
CCATTGGATC CCCGGAGGAC CCCAAAAATG TCTGAGCCAA GGTCAAAAAC CAGTGGTAGA 
ACTGATTCTA GCACTACTTC TGGAGTAGCC AGGGATACCC CAGTTGCGAC TACCCCAACA 
GGAGGAATCA CTAAGGAGAT GAAGGAGGCT GACAATCGCA GACAGGCTGC AGCTGCTGCT 
GCTGCCTCTG CCGCCGCTGC TACCACCTCT GCATCCACTG CCTCTGAAAG TGGAATGGAT 
AAAGTTGTGT CTCAGCTCGC CACTGTGGCC AAGAGTGTGC TTGGACCTGT CAAGCTCAAT 
ATCCCAGGTA CTAAAGAGAC CATGGAACGA ACAAAGGACC AGCGTACCCA GGCAGATGCC 
ACCCACCCAA AGATAAAAGA ATGTCGCCTG GAGCACCCTG ATTCTCCTGA CCAATTGAGT 
CCATCTGCTG CTTCGGGCTC CCAGTCTCCA CGACTGACTG AATCTCCATC TAAACAAACA 
TCATCCAAAT CTGAGCCAGC CTCAACTTCT CAGAGACCTT TAGAGGTCCC AGGGACTTTC 
AAAAGACAAA CTTCTCCAAT CCCTCAAGCT AGGGAAGAGC ATCAGGATAG GTCAGGAACT 
CTGCAAAGTG GCACCACATC AAGGCCTAGG TCTGAAGCAC AATCACAGAC CCAGCAGGCA 
AAGCCCAGTT CCACTACGTC ACGAGATAAA TCCAACAAGA CAACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_021471798.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327473.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X6, mRNA.

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CloneID	ODa144726
Clone ID Related Accession (Same CDS sequence)	XM_021471798.1
Accession Version	XM_021471798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394654.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA TCCTCACTGG AAAGCAGCTT CTGCCTCTGT CCAGCAGTAT GCATGGAGGA 
GTGAGTCAGC TGGCCTGGCA GCAGCACACA GGGGAGCCCA ATAACTTGGT CCGCATGAGG 
AGTCAGTCCT TTGGCCAGTC TGCCCCGTCT CTCACTGCGG GACTGAAGGA GCTGAGTCTT 
CCTAGAAGAG GCAGTTTCTG TCGGACGAGC AACCGAAAAA GTTTGATAGT GACATCCAGC 
ACGTCTCCAA CGCTGCCTCG ACCCCATTCA CCACTGCACG GGCATCCAGG AAGCAGCCCA 
TTGGACAGCC CTCGCAATTT CTCCCCCAAC ACTGCAGCCC ACTTCTCTTT TGTACCAGCT 
CGAAGCCATG GACACAGGGC TGACAGGACT GATGGTCGTC GCTGGTCTCT CGCCTCTCTG 
CCGTCCTCTG GCTATGGAAC AAACACCCCC AGTTCGACAG TCTCTTCATC ATGTTCATCT 
CAGGAGAAGC TGCACCAGTT GCCCTTCCAG CCCACTGCAG ACGAGCTGCA TTTCCTCACC 
AAACACTTCA GCTCGGAGAG CATCACGGAT GAGGATGGGA GACGATCCCC AGCCATGCGT 
CCGCGCTCCC GCAGTCTCAG CCCTGGACGG TCACCAATAT CATTTGACCA TGAGATTGTC 
ATGATGAACC ATGTCTACAA GGAGCGTTTT CCTAAGGCCA CTGCTCAGAT GGAGGTGCGT 
CTGGCCGATT TCATATCCTC CTCAGCTCCA GAAAAAGTCA TGCCTTTAGC TGATGGCGTC 
CTGAGCTTCA TCCACCACCA GGTTATCGAA CTGTCTCGTG ACTGTCTGGA CAAATCTCGT 
GAGGGTCTCA TCACCTCCCG CTACTTTTAT GAGCTACAAG AGAACCTCGA AAAGCTACAC 
CAGGATGCTC ATGAGCGTTC AGAAAGTGGG GAGGTGACAT TTGTCACCCA GCTAGTTAAA 
AAGCTGATGA TAATCATCGC TCGGCCTGCC CGGCTGCTAG AATGTTTGGA GTTTGACCCG 
GAGGAGTTCT ACCACCTGTT AGAGGCAGCT GAAGGTCATG CTAAAGAAGG TCAAGGCATC 
AAGTCTGACA TCCCTCGATA CATCATCAGC CAGCTGGGCC TCACCAGGGA CCCCCTTGAG 
GAAATGGCCC AGCTGAGCAG CTATGACAGC GGTAACCCTG AAACACCAGA GACAGAGACA 
GATTCCACAG ATAGTCGAGG AGCAACCGTA CAGCCACTCC AACCTCAGCC TCAACCGCAG 
CCCAAGACTA AAACACCTCG CGAGGAAGAC TTCGAGACCA TTAAACTCAT CAGTAATGGA 
GCCTATGGGG CTGTGTTTCT GGTGAGGCAT AAAGAGACCC GTCAGCGATT TGCCATGAAG 
AAGATCAACA AGCAGAACCT GATCCTAAGA AACCAGATCC AGCAGGCCTT TGTGGAAAGA 
GACATCTTGA CATTTGCAGA AAACCCTTTT GTTGTCAGCA TGTTCTGCTC CTTTGAGACA 
AGGAGACATC TTTGCATGGT TATGGAGTAT GTGGAGGGTG GGGATTGTGC CACTCTCCTG 
AAGCACATTG GAGCTCTGCC TGTGGACATG GCACGCATGT ACTTTGCTGA AACCGTTCTT 
GCATTGGAAT ACCTTCACAA CTATGGTATT GTGCACAGAG ACCTCAAACC TGACAATCTC 
CTGATTACCT CAATGGGACA CGTCAAACTG ACTGACTTTG GCCTGTCCAA GATTGGTTTA 
ATGAGCTTAA CAACTAATCT GTATGAGGGC CACATTGAAA AAGATACTCG AGAATTCTTG 
GACAAACAGG TGTGTGGTAC TCCTGAATAC ATTGCTCCTG AGGTTATTCT GCGACAGGGT 
TATGGAAAAC CAGTTGACTG GTGGGCCATG GGAGTCATCT TGTATGAGTT TCTGGTGGGC 
TGTGCTCCTT TCTTTGGAGA TACACCTGAA GAACTGTTTG GACAAGTCAT CAGTGATGAG 
ATAATCTGGC CAGAAGGTGA TGAAGCTTTG CCTCCGGATG CTCAAGATCT CATCTCCAAA 
CTTCTTCGGC AAAACCCTCT AGAGCGTCTT GGGACAGGGA GTGCGTTTGA GGTAAAACAG 
CACCGCTTCT TTACTGATCT GGACTGGAAC AGCCTCCTGA GGCAGAAGGC CGAGTTCATT 
CCTCAGCTTG AGTCGGAGGA TGACACTAGC TACTTTGACA CACGCTCAGA TCGTTATCAT 
CATGTGGACT CAGAAGAAGA AGATGACACA AATGATGACG ACCATTTGGA GATCCGTCAG 
TTCTCCTCCT GTTCGCCACG ATTCAGCAAG GTGTATAGTA GTATGGAGCG TCTTTCCTTG 
CATGAGGAGA AACGCACCCC TCCTCCTACT AAGCGCAGTC TGAGTGAAGA GGGAGGAGAG 
CGGATCGACA GTCTAAGTGG CCTAAAGTCC AGAGACCGAA GCTGGTTGGT GGGCTCACCT 
GAAATTCTCA GAAAGCGTCT CTCTGTTTCC GAGTCTTCTC ACACCGAGAG TGACTCGAGT 
CCACCTCTGA CTGTTCGTCG CCGCTGCTGC TCGGCCATCA TTGAAATGCC TCGCTTCGCC 
ATATCCAGTG AAGAGGATAG CTGTGTGGGG AGCAGGAAGA CCCCTGTGAG AGGCCCTCGA 
GGGGATGATC TGCCTCAGGC CATACCAGAG CTCCCTGTAG AGAGAGAGCT CAGGCTGGAT 
GAATCGCCCA CCACGCCAGG ATCCACTTCC AGTCAAATCA GCCAGGCCAC ACTCACACCT 
GGTAGTTCTG GTGATGTGTT AGATCGTGCT TCTCGCTATA GTGCTGAAGT TTCTGAAAAC 
TCGACTCCAA GAGCCATCAG TGATTTGGCG GCTCGAAGAG CTCGCCACAG ACTGCTTTCA 
GGAGATACAG ATAAACACAC ATCACGTCCA CTTAGCAAAG TCATCAAATC AGCTTCAGCA 
ACAACCCTGT CACTCATGAT CCCTGCTGAT CACCATGGTG CTTCTCCACT GGCCAGTCCC 
ATGTCGCCTC ACTCTCTCTC TTCGAACCCC TCATCACGTG ACTCCTCGCC CAGCCGTGAC 
CTTTCCCCAG CTGTCTGTAG TGTTAAACCT GCCATAATCA TCCACCGAGC TGGAAAGAAA 
TATGGTTTTA CTTTACGTGC AATCCGGGTT TACATGGGAG ACACAGACAT ATATACTGTC 
CATCACATGG TCTGGCATGT GGAGGATGGA GGACCTGCTC ATGAGGCTGG ACTGAGAGAG 
GGAGATCTCA TCACACATGT TAACGGAGAG CCTGTCCATG GACTTGTGCA CACAGAAGTT 
GTTGAGCTCA TTCTTAAGAG TGGCAGTAAG GTGTCCATCT CTGCTACACC CTTTGAAAAT 
ACATCAATTA AGGTTGGTCC TGCTCGCAAA ACTAGCTACA AGTCCAAAAT GGCCCGTCGC 
AACAAAAGAA CCAAAACTAG AGAAGGACAA GACAGTAAGA AGAGGAATTC CCTCTTCCGT 
AAGATCACCA AACAGGCGAC CCTTCTTCAC ACCAGTCGCA GCCTGTCCTC TCTGAATCGC 
TCGGTCTCTT CTGGTGAGAG CGTCCCAGGC TCTCCTACTC ACATGTCGCC TCGTTCGCCC 
ACACAGGGCT GCCGTTCAAC ACCTGACTCC GCCCACTCAG TTGGTGGAAA CTCATCCCAA 
AGTAGCTCGC CTAGCTCCAG TGTGCCAAAC TCTCCTGCCA GTTCTGGCCA GATCAGACCC 
AGCTCCTTGC ATGGTCTGGC CCCCAAGCTG CAGCGCCAGT ACCGCTCCCC CCGCCGGAAG 
TCTGCAGGCA ACATCCCTTT ATCCCCTCTG GCTCGTACAC CATCGCCCAC TCCACAGAGC 
ACTTCTCCCC AGCGTTCCCC TTCTCCCCTG CCAAGCCATG GGCTCATCAC TTCTTCAATA 
GGCCAGTCTT TTCCTGTCAA ACTGCACTCT TCACCTCCAT TAGTAAGACA GATATCTCGG 
CCAAAGAGTG CAGACCCTCC TCGCTCTCCT TTGCTGAAAC GAGTCCAGTC CGCAGAGAAA 
CTCGCTGCCT CTCTTTCTTC TTCATCCTCC TCTCCTTCTC CCTCTTCAGC TGCTGATAAA 
AAGCTCCCAG TTGGTGCCAG CCGCAAACAC AGTCTTGATA CCTCACATTC AGAGTTTAAA 
AAAGAGATGC TGCAGCGAGA CCCTAGTCTA CAGAGCCTGC AAGAGTCAGC CAGTGAGACT 
CTTATGGGAG GAAGAGTGTC TCCTGCAGAA AAGGGAAGCC TACAGAAAAG TTCAGTGCGT 
AAACTGGGCA GGCAAGAAGG TCCGGAGTCT GGAACGGGAA CTCTGGGACT TGTTCCTGGA 
AAGAGTAAAT TGAAAGACAA GCTGTCTGCC ATACGTGCTG AACGCCGCGA ATCTCTACAA 
AAGCAGGATG CCATCCATGA AGTGGATTCA TCTGAAGATG AGACAGACGA AGGGTCGGAA 
GACAGCCAAG ATGGGCGCAG AACTTGCTAT ACTCCTCCTA GCCATGTCTT GAGGCCTACA 
ACTGTAATGG CCTCCCCCCA TACCATCAGA ATTGGGCCAG CGGCTCCACC AACACTGGGA 
CTTTTTCCTT CAACAGTTGC ACCGCCAAGC TCACCTCAGC TCATCAGAGG ACTGCAGATA 
CAAAACCAAA GTACCACTCA ATCTCAGACA CAAACTTCAA CCTCACAACC AGCGCAAGCT 
GCAGTTAAAC CCCCAGAACA GAAACCAATA ACCTCGGTTA AAGACTCAAG TTCAACGATA 
ATTGATGATT CGAAAAAACA GTCACAGGCA CAAGACATTC AGTCCAGACC TTCTCCACAT 
CAACCCAGTC CTCTGTCTAG TACCTTTTCC ACCCAAGGCA GCGCCTTTAC CTCTGTTAGT 
AAAGACCCAT TTGTCAAGCC CACCACACCT CCATTGGATC CCCGGAGGAC CCCAAAAATG 
TCTGAGCCAA GGTCAAAAAC CAGTGGTAGA ACTGATTCTA GCACTACTTC TGGAGTAGCC 
AGGGATACCC CAGTTGCTAC TACCCCAACA GGAGGAATCA CTAAGGAGAT GAAGGAGGCT 
GACAATCGCA GACAGGCTGC AGCTGCTGCT GCTGCCTCTG CTGCCGCTGC TACCACCTCT 
GCATCCACTG CCTCTGAAAG TGGAATGGAT AAAGTTGTGT CTCAGCTCGC CACTGTGGCC 
AAGAGTGTGC TTGGACCTGT CAAGCTCAAT ATCCCAGGTA CTAAAGAGAC CATGGAACGA 
ACAAAGGACC AGCGTACCCA GGCAGATGCC ACCCACCCAA AGATAAAAGA ATGTCGCCTG 
GAGCACCCTG ATTCTCCTGA CCAATTGAGT CCATCTGCTG CTTCGGGCTC CCAGTCTCCA 
CGACTGACTG AATCTCCATC TAAACAAACA TCATCCAAAT ATGAGCCAGC CTCAACTTCT 
CAGAGACCTT TAGAGGTCCC AGGGACTTTC AAAAGACAAA CTTCTCCAAT CCCTCAAGCT 
AGGGAAGAGC ATCAGGATAG GTCAGGAACT CTGCAAAGTG GCACCACATC AAGGCCTAGG 
TCTGAAGCAC AATCACAGAC CCAGCAGGCA AAGCCCAGTT CCACTACGTC ACGAGATAAA 
TCCAACAAGA CAACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327473.1
CDS	123..5720
Translation	MAGILTGKQLLPLSSSMHGGVSQLAWQQHTGEPNNLVRMRSQSFGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQATLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYTPPSHVLRPTTVMASPHTIRIGPAAPPTLGLFPSTVAPPSSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKYEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_021471798.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471798.1

ORF Insert Sequence:

ATGGCTGGCA TCCTCACTGG AAAGCAGCTT CTGCCTCTGT CCAGCAGTAT GCATGGAGGA 
GTGAGTCAGC TGGCCTGGCA GCAGCACACA GGGGAGCCCA ATAACTTGGT CCGCATGAGG 
AGTCAGTCCT TTGGCCAGTC TGCCCCGTCT CTCACTGCGG GACTGAAGGA GCTGAGTCTT 
CCTAGAAGAG GCAGTTTCTG TCGGACGAGC AACCGAAAAA GTTTGATAGT GACATCCAGC 
ACGTCTCCAA CGCTGCCTCG ACCCCATTCA CCACTGCACG GGCATCCAGG AAGCAGCCCA 
TTGGACAGCC CTCGCAATTT CTCCCCCAAC ACTGCAGCCC ACTTCTCTTT TGTACCAGCT 
CGAAGCCATG GACACAGGGC TGACAGGACT GATGGTCGTC GCTGGTCTCT CGCCTCTCTG 
CCGTCCTCTG GCTATGGAAC AAACACCCCC AGTTCGACAG TCTCTTCATC ATGTTCATCT 
CAGGAGAAGC TGCACCAGTT GCCCTTCCAG CCCACTGCAG ACGAGCTGCA TTTCCTCACC 
AAACACTTCA GCTCGGAGAG CATCACGGAT GAGGATGGGA GACGATCCCC AGCCATGCGT 
CCGCGCTCCC GCAGTCTCAG CCCTGGACGG TCACCAATAT CATTTGACCA TGAGATTGTC 
ATGATGAACC ATGTCTACAA GGAGCGTTTT CCTAAGGCCA CTGCTCAGAT GGAGGTGCGT 
CTGGCCGATT TCATATCCTC CTCAGCTCCA GAAAAAGTCA TGCCTTTAGC TGATGGCGTC 
CTGAGCTTCA TCCACCACCA GGTTATCGAA CTGTCTCGTG ACTGTCTGGA CAAATCTCGT 
GAGGGTCTCA TCACCTCCCG CTACTTTTAT GAGCTACAAG AGAACCTCGA AAAGCTACAC 
CAGGATGCTC ATGAGCGTTC AGAAAGTGGG GAGGTGACAT TTGTCACCCA GCTAGTTAAA 
AAGCTGATGA TAATCATCGC TCGGCCTGCC CGGCTGCTAG AATGTTTGGA GTTTGACCCG 
GAGGAGTTCT ACCACCTGTT AGAGGCAGCT GAAGGTCATG CTAAAGAAGG TCAAGGCATC 
AAGTCTGACA TCCCTCGATA CATCATCAGC CAGCTGGGCC TCACCAGGGA CCCCCTTGAG 
GAAATGGCCC AGCTGAGCAG CTATGACAGC GGTAACCCTG AAACACCAGA GACAGAGACA 
GATTCCACAG ATAGTCGAGG AGCAACCGTA CAGCCACTCC AACCTCAGCC TCAACCGCAG 
CCCAAGACTA AAACACCTCG CGAGGAAGAC TTCGAGACCA TTAAACTCAT CAGTAATGGA 
GCCTATGGGG CTGTGTTTCT GGTGAGGCAT AAAGAGACCC GTCAGCGATT TGCCATGAAG 
AAGATCAACA AGCAGAACCT GATCCTAAGA AACCAGATCC AGCAGGCCTT TGTGGAAAGA 
GACATCTTGA CATTTGCAGA AAACCCTTTT GTTGTCAGCA TGTTCTGCTC CTTTGAGACA 
AGGAGACATC TTTGCATGGT TATGGAGTAT GTGGAGGGTG GGGATTGTGC CACTCTCCTG 
AAGCACATTG GAGCTCTGCC TGTGGACATG GCACGCATGT ACTTTGCTGA AACCGTTCTT 
GCATTGGAAT ACCTTCACAA CTATGGTATT GTGCACAGAG ACCTCAAACC TGACAATCTC 
CTGATTACCT CAATGGGACA CGTCAAACTG ACTGACTTTG GCCTGTCCAA GATTGGTTTA 
ATGAGCTTAA CAACTAATCT GTATGAGGGC CACATTGAAA AAGATACTCG AGAATTCTTG 
GACAAACAGG TGTGTGGTAC TCCTGAATAC ATTGCTCCTG AGGTTATTCT GCGACAGGGT 
TATGGAAAAC CAGTTGACTG GTGGGCCATG GGAGTCATCT TGTATGAGTT TCTGGTGGGC 
TGTGCTCCTT TCTTTGGAGA TACACCTGAA GAACTGTTTG GACAAGTCAT CAGTGATGAG 
ATAATCTGGC CAGAAGGTGA TGAAGCTTTG CCTCCGGATG CTCAAGATCT CATCTCCAAA 
CTTCTTCGGC AAAACCCTCT AGAGCGTCTT GGGACAGGGA GTGCGTTTGA GGTAAAACAG 
CACCGCTTCT TTACTGATCT GGACTGGAAC AGCCTCCTGA GGCAGAAGGC CGAGTTCATT 
CCTCAGCTTG AGTCGGAGGA TGACACTAGC TACTTTGACA CACGCTCAGA TCGTTATCAT 
CATGTGGACT CAGAAGAAGA AGATGACACA AATGATGACG ACCATTTGGA GATCCGTCAG 
TTCTCCTCCT GTTCGCCACG ATTCAGCAAG GTGTATAGTA GTATGGAGCG TCTTTCCTTG 
CATGAGGAGA AACGCACCCC TCCTCCTACT AAGCGCAGTC TGAGTGAAGA GGGAGGAGAG 
CGGATCGACA GTCTAAGTGG CCTAAAGTCC AGAGACCGAA GCTGGTTGGT GGGCTCACCT 
GAAATTCTCA GAAAGCGTCT CTCTGTTTCC GAGTCTTCTC ACACCGAGAG TGACTCGAGT 
CCACCTCTGA CTGTTCGTCG CCGCTGCTGC TCGGCCATCA TTGAAATGCC TCGCTTCGCC 
ATATCCAGTG AAGAGGATAG CTGTGTGGGG AGCAGGAAGA CCCCTGTGAG AGGCCCTCGA 
GGGGATGATC TGCCTCAGGC CATACCAGAG CTCCCTGTAG AGAGAGAGCT CAGGCTGGAT 
GAATCGCCCA CCACGCCAGG ATCCACTTCC AGTCAAATCA GCCAGGCCAC ACTCACACCT 
GGTAGTTCTG GTGATGTGTT AGATCGTGCT TCTCGCTATA GTGCTGAAGT TTCTGAAAAC 
TCGACTCCAA GAGCCATCAG TGATTTGGCG GCTCGAAGAG CTCGCCACAG ACTGCTTTCA 
GGAGATACAG ATAAACACAC ATCACGTCCA CTTAGCAAAG TCATCAAATC AGCTTCAGCA 
ACAACCCTGT CACTCATGAT CCCTGCTGAT CACCATGGTG CTTCTCCACT GGCCAGTCCC 
ATGTCGCCTC ACTCTCTCTC TTCGAACCCC TCATCACGTG ACTCCTCGCC CAGCCGTGAC 
CTTTCCCCAG CTGTCTGTAG TGTTAAACCT GCCATAATCA TCCACCGAGC TGGAAAGAAA 
TATGGTTTTA CTTTACGTGC AATCCGGGTT TACATGGGAG ACACAGACAT ATATACTGTC 
CATCACATGG TCTGGCATGT GGAGGATGGA GGACCTGCTC ATGAGGCTGG ACTGAGAGAG 
GGAGATCTCA TCACACATGT TAACGGAGAG CCTGTCCATG GACTTGTGCA CACAGAAGTT 
GTTGAGCTCA TTCTTAAGAG TGGCAGTAAG GTGTCCATCT CTGCTACACC CTTTGAAAAT 
ACATCAATTA AGGTTGGTCC TGCTCGCAAA ACTAGCTACA AGTCCAAAAT GGCCCGTCGC 
AACAAAAGAA CCAAAACTAG AGAAGGACAA GACAGTAAGA AGAGGAATTC CCTCTTCCGT 
AAGATCACCA AACAGGCGAC CCTTCTTCAC ACCAGTCGCA GCCTGTCCTC TCTGAATCGC 
TCGGTCTCTT CTGGTGAGAG CGTCCCAGGC TCTCCTACTC ACATGTCGCC TCGTTCGCCC 
ACACAGGGCT GCCGTTCAAC ACCTGACTCC GCCCACTCAG TTGGTGGAAA CTCATCCCAA 
AGTAGCTCGC CTAGCTCCAG TGTGCCAAAC TCTCCTGCCA GTTCTGGCCA GATCAGACCC 
AGCTCCTTGC ATGGTCTGGC CCCCAAGCTG CAGCGCCAGT ACCGCTCCCC CCGCCGGAAG 
TCTGCAGGCA ACATCCCTTT ATCCCCTCTG GCTCGTACAC CATCGCCCAC TCCACAGAGC 
ACTTCTCCCC AGCGTTCCCC TTCTCCCCTG CCAAGCCATG GGCTCATCAC TTCTTCAATA 
GGCCAGTCTT TTCCTGTCAA ACTGCACTCT TCACCTCCAT TAGTAAGACA GATATCTCGG 
CCAAAGAGTG CAGACCCTCC TCGCTCTCCT TTGCTGAAAC GAGTCCAGTC CGCAGAGAAA 
CTCGCTGCCT CTCTTTCTTC TTCATCCTCC TCTCCTTCTC CCTCTTCAGC TGCTGATAAA 
AAGCTCCCAG TTGGTGCCAG CCGCAAACAC AGTCTTGATA CCTCACATTC AGAGTTTAAA 
AAAGAGATGC TGCAGCGAGA CCCTAGTCTA CAGAGCCTGC AAGAGTCAGC CAGTGAGACT 
CTTATGGGAG GAAGAGTGTC TCCTGCAGAA AAGGGAAGCC TACAGAAAAG TTCAGTGCGT 
AAACTGGGCA GGCAAGAAGG TCCGGAGTCT GGAACGGGAA CTCTGGGACT TGTTCCTGGA 
AAGAGTAAAT TGAAAGACAA GCTGTCTGCC ATACGTGCTG AACGCCGCGA ATCTCTACAA 
AAGCAGGATG CCATCCATGA AGTGGATTCA TCTGAAGATG AGACAGACGA AGGGTCGGAA 
GACAGCCAAG ATGGGCGCAG AACTTGCTAT ACTCCTCCTA GCCATGTCTT GAGGCCTACA 
ACTGTAATGG CCTCCCCCCA TACCATCAGA ATTGGGCCAG CGGCTCCACC AACACTGGGA 
CTTTTTCCTT CAACAGTTGC ACCGCCAAGC TCACCTCAGC TCATCAGAGG ACTGCAGATA 
CAAAACCAAA GTACCACTCA ATCTCAGACA CAAACTTCAA CCTCACAACC AGCGCAAGCT 
GCAGTTAAAC CCCCAGAACA GAAACCAATA ACCTCGGTTA AAGACTCAAG TTCAACGATA 
ATTGATGATT CGAAAAAACA GTCACAGGCA CAAGACATTC AGTCCAGACC TTCTCCACAT 
CAACCCAGTC CTCTGTCTAG TACCTTTTCC ACCCAAGGCA GCGCCTTTAC CTCTGTTAGT 
AAAGACCCAT TTGTCAAGCC CACCACACCT CCATTGGATC CCCGGAGGAC CCCAAAAATG 
TCTGAGCCAA GGTCAAAAAC CAGTGGTAGA ACTGATTCTA GCACTACTTC TGGAGTAGCC 
AGGGATACCC CAGTTGCTAC TACCCCAACA GGAGGAATCA CTAAGGAGAT GAAGGAGGCT 
GACAATCGCA GACAGGCTGC AGCTGCTGCT GCTGCCTCTG CTGCCGCTGC TACCACCTCT 
GCATCCACTG CCTCTGAAAG TGGAATGGAT AAAGTTGTGT CTCAGCTCGC CACTGTGGCC 
AAGAGTGTGC TTGGACCTGT CAAGCTCAAT ATCCCAGGTA CTAAAGAGAC CATGGAACGA 
ACAAAGGACC AGCGTACCCA GGCAGATGCC ACCCACCCAA AGATAAAAGA ATGTCGCCTG 
GAGCACCCTG ATTCTCCTGA CCAATTGAGT CCATCTGCTG CTTCGGGCTC CCAGTCTCCA 
CGACTGACTG AATCTCCATC TAAACAAACA TCATCCAAAT ATGAGCCAGC CTCAACTTCT 
CAGAGACCTT TAGAGGTCCC AGGGACTTTC AAAAGACAAA CTTCTCCAAT CCCTCAAGCT 
AGGGAAGAGC ATCAGGATAG GTCAGGAACT CTGCAAAGTG GCACCACATC AAGGCCTAGG 
TCTGAAGCAC AATCACAGAC CCAGCAGGCA AAGCCCAGTT CCACTACGTC ACGAGATAAA 
TCCAACAAGA CAACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_021471799.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327474.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X7, mRNA.

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CloneID	ODa144727
Clone ID Related Accession (Same CDS sequence)	XM_021471799.1
Accession Version	XM_021471799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394654.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA TCCTCACTGG AAAGCAGCTT CTGCCTCTGT CCAGCAGTAT GCATGGAGGA 
GTGAGTCAGC TGGCCTGGCA GCAGCACACA GGGGAGCCCA ATAACTTGGT CCGCATGAGG 
AGTCAGTCCT TTGGCCAGTC TGCCCCGTCT CTCACTGCGG GACTGAAGGA GCTGAGTCTT 
CCTAGAAGAG GCAGTTTCTG TCGGACGAGC AACCGAAAAA GTTTGATAGT GACATCCAGC 
ACGTCTCCAA CGCTGCCTCG ACCCCATTCA CCACTGCACG GGCATCCAGG AAGCAGCCCA 
TTGGACAGCC CTCGCAATTT CTCCCCCAAC ACTGCAGCCC ACTTCTCTTT TGTACCAGCT 
CGAAGGACTG ATGGTCGTCG CTGGTCTCTC GCCTCTCTGC CGTCCTCTGG CTATGGAACA 
AACACCCCCA GTTCGACAGT CTCTTCATCA TGTTCATCTC AGGAGAAGCT GCACCAGTTG 
CCCTTCCAGC CCACTGCAGA CGAGCTGCAT TTCCTCACCA AACACTTCAG CTCGGAGAGC 
ATCACGGATG AGGATGGGAG ACGATCCCCA GCCATGCGTC CGCGCTCCCG CAGTCTCAGC 
CCTGGACGGT CACCAATATC ATTTGACCAT GAGATTGTCA TGATGAACCA TGTCTACAAG 
GAGCGTTTTC CTAAGGCCAC TGCTCAGATG GAGGTGCGTC TGGCCGATTT CATATCCTCC 
TCAGCTCCAG AAAAAGTCAT GCCTTTAGCT GATGGCGTCC TGAGCTTCAT CCACCACCAG 
GTTATCGAAC TGTCTCGTGA CTGTCTGGAC AAATCTCGTG AGGGTCTCAT CACCTCCCGC 
TACTTTTATG AGCTACAAGA GAACCTCGAA AAGCTACACC AGGATGCTCA TGAGCGTTCA 
GAAAGTGGGG AGGTGACATT TGTCACCCAG CTAGTTAAAA AGCTGATGAT AATCATCGCT 
CGGCCTGCCC GGCTGCTAGA ATGTTTGGAG TTTGACCCGG AGGAGTTCTA CCACCTGTTA 
GAGGCAGCTG AAGGTCATGC TAAAGAAGGT CAAGGCATCA AGTCTGACAT CCCTCGATAC 
ATCATCAGCC AGCTGGGCCT CACCAGGGAC CCCCTTGAGG AAATGGCCCA GCTGAGCAGC 
TATGACAGCG GTAACCCTGA AACACCAGAG ACAGAGACAG ATTCCACAGA TAGTCGAGGA 
GCAACCGTAC AGCCACTCCA ACCTCAGCCT CAACCGCAGC CCAAGACTAA AACACCTCGC 
GAGGAAGACT TCGAGACCAT TAAACTCATC AGTAATGGAG CCTATGGGGC TGTGTTTCTG 
GTGAGGCATA AAGAGACCCG TCAGCGATTT GCCATGAAGA AGATCAACAA GCAGAACCTG 
ATCCTAAGAA ACCAGATCCA GCAGGCCTTT GTGGAAAGAG ACATCTTGAC ATTTGCAGAA 
AACCCTTTTG TTGTCAGCAT GTTCTGCTCC TTTGAGACAA GGAGACATCT TTGCATGGTT 
ATGGAGTATG TGGAGGGTGG GGATTGTGCC ACTCTCCTGA AGCACATTGG AGCTCTGCCT 
GTGGACATGG CACGCATGTA CTTTGCTGAA ACCGTTCTTG CATTGGAATA CCTTCACAAC 
TATGGTATTG TGCACAGAGA CCTCAAACCT GACAATCTCC TGATTACCTC AATGGGACAC 
GTCAAACTGA CTGACTTTGG CCTGTCCAAG ATTGGTTTAA TGAGCTTAAC AACTAATCTG 
TATGAGGGCC ACATTGAAAA AGATACTCGA GAATTCTTGG ACAAACAGGT GTGTGGTACT 
CCTGAATACA TTGCTCCTGA GGTTATTCTG CGACAGGGTT ATGGAAAACC AGTTGACTGG 
TGGGCCATGG GAGTCATCTT GTATGAGTTT CTGGTGGGCT GTGCTCCTTT CTTTGGAGAT 
ACACCTGAAG AACTGTTTGG ACAAGTCATC AGTGATGAGA TAATCTGGCC AGAAGGTGAT 
GAAGCTTTGC CTCCGGATGC TCAAGATCTC ATCTCCAAAC TTCTTCGGCA AAACCCTCTA 
GAGCGTCTTG GGACAGGGAG TGCGTTTGAG GTAAAACAGC ACCGCTTCTT TACTGATCTG 
GACTGGAACA GCCTCCTGAG GCAGAAGGCC GAGTTCATTC CTCAGCTTGA GTCGGAGGAT 
GACACTAGCT ACTTTGACAC ACGCTCAGAT CGTTATCATC ATGTGGACTC AGAAGAAGAA 
GATGACACAA ATGATGACGA CCATTTGGAG ATCCGTCAGT TCTCCTCCTG TTCGCCACGA 
TTCAGCAAGG TGTATAGTAG TATGGAGCGT CTTTCCTTGC ATGAGGAGAA ACGCACCCCT 
CCTCCTACTA AGCGCAGTCT GAGTGAAGAG GGAGGAGAGC GGATCGACAG TCTAAGTGGC 
CTAAAGTCCA GAGACCGAAG CTGGTTGGTG GGCTCACCTG AAATTCTCAG AAAGCGTCTC 
TCTGTTTCCG AGTCTTCTCA CACCGAGAGT GACTCGAGTC CACCTCTGAC TGTTCGTCGC 
CGCTGCTGCT CGGCCATCAT TGAAATGCCT CGCTTCGCCA TATCCAGTGA AGAGGATAGC 
TGTGTGGGGA GCAGGAAGAC CCCTGTGAGA GGCCCTCGAG GGGATGATCT GCCTCAGGCC 
ATACCAGAGC TCCCTGTAGA GAGAGAGCTC AGGCTGGATG AATCGCCCAC CACGCCAGGA 
TCCACTTCCA GTCAAATCAG CCAGGCCACA CTCACACCTG GTAGTTCTGG TGATGTGTTA 
GATCGTGCTT CTCGCTATAG TGCTGAAGTT TCTGAAAACT CGACTCCAAG AGCCATCAGT 
GATTTGGCGG CTCGAAGAGC TCGCCACAGA CTGCTTTCAG GAGATACAGA TAAACACACA 
TCACGTCCAC TTAGCAAAGT CATCAAATCA GCTTCAGCAA CAACCCTGTC ACTCATGATC 
CCTGCTGATC ACCATGGTGC TTCTCCACTG GCCAGTCCCA TGTCGCCTCA CTCTCTCTCT 
TCGAACCCCT CATCACGTGA CTCCTCGCCC AGCCGTGACC TTTCCCCAGC TGTCTGTAGT 
GTTAAACCTG CCATAATCAT CCACCGAGCT GGAAAGAAAT ATGGTTTTAC TTTACGTGCA 
ATCCGGGTTT ACATGGGAGA CACAGACATA TATACTGTCC ATCACATGGT CTGGCATGTG 
GAGGATGGAG GACCTGCTCA TGAGGCTGGA CTGAGAGAGG GAGATCTCAT CACACATGTT 
AACGGAGAGC CTGTCCATGG ACTTGTGCAC ACAGAAGTTG TTGAGCTCAT TCTTAAGAGT 
GGCAGTAAGG TGTCCATCTC TGCTACACCC TTTGAAAATA CATCAATTAA GGTTGGTCCT 
GCTCGCAAAA CTAGCTACAA GTCCAAAATG GCCCGTCGCA ACAAAAGAAC CAAAACTAGA 
GAAGGACAAG ACAGTAAGAA GAGGAATTCC CTCTTCCGTA AGATCACCAA ACAGGCGACC 
CTTCTTCACA CCAGTCGCAG CCTGTCCTCT CTGAATCGCT CGGTCTCTTC TGGTGAGAGC 
GTCCCAGGCT CTCCTACTCA CATGTCGCCT CGTTCGCCCA CACAGGGCTG CCGTTCAACA 
CCTGACTCCG CCCACTCAGT TGGTGGAAAC TCATCCCAAA GTAGCTCGCC TAGCTCCAGT 
GTGCCAAACT CTCCTGCCAG TTCTGGCCAG ATCAGACCCA GCTCCTTGCA TGGTCTGGCC 
CCCAAGCTGC AGCGCCAGTA CCGCTCCCCC CGCCGGAAGT CTGCAGGCAA CATCCCTTTA 
TCCCCTCTGG CTCGTACACC ATCGCCCACT CCACAGAGCA CTTCTCCCCA GCGTTCCCCT 
TCTCCCCTGC CAAGCCATGG GCTCATCACT TCTTCAATAG GCCAGTCTTT TCCTGTCAAA 
CTGCACTCTT CACCTCCATT AGTAAGACAG ATATCTCGGC CAAAGAGTGC AGACCCTCCT 
CGCTCTCCTT TGCTGAAACG AGTCCAGTCC GCAGAGAAAC TCGCTGCCTC TCTTTCTTCT 
TCATCCTCCT CTCCTTCTCC CTCTTCAGCT GCTGATAAAA AGCTCCCAGT TGGTGCCAGC 
CGCAAACACA GTCTTGATAC CTCACATTCA GAGTTTAAAA AAGAGATGCT GCAGCGAGAC 
CCTAGTCTAC AGAGCCTGCA AGAGTCAGCC AGTGAGACTC TTATGGGAGG AAGAGTGTCT 
CCTGCAGAAA AGGGAAGCCT ACAGAAAAGT TCAGTGCGTA AACTGGGCAG GCAAGAAGGT 
CCGGAGTCTG GAACGGGAAC TCTGGGACTT GTTCCTGGAA AGAGTAAATT GAAAGACAAG 
CTGTCTGCCA TACGTGCTGA ACGCCGCGAA TCTCTACAAA AGCAGGATGC CATCCATGAA 
GTGGATTCAT CTGAAGATGA GACAGACGAA GGGTCGGAAG ACAGCCAAGA TGGGCGCAGA 
ACTTGCTATA CTCCTCCTAG CCATGTCTTG AGGCCTACAA CTGTAATGGC CTCCCCCCAT 
ACCATCAGAA TTGGGCCAGC GGCTCCACCA ACACTGGGAC TTTTTCCTTC AACAGTTGCA 
CCGCCAAGCT CACCTCAGCT CATCAGAGGA CTGCAGATAC AAAACCAAAG TACCACTCAA 
TCTCAGACAC AAACTTCAAC CTCACAACCA GCGCAAGCTG CAGTTAAACC CCCAGAACAG 
AAACCAATAA CCTCGGTTAA AGACTCAAGT TCAACGATAA TTGATGATTC GAAAAAACAG 
TCACAGGCAC AAGACATTCA GTCCAGACCT TCTCCACATC AACCCAGTCC TCTGTCTAGT 
ACCTTTTCCA CCCAAGGCAG CGCCTTTACC TCTGTTAGTA AAGACCCATT TGTCAAGCCC 
ACCACACCTC CATTGGATCC CCGGAGGACC CCAAAAATGT CTGAGCCAAG GTCAAAAACC 
AGTGGTAGAA CTGATTCTAG CACTACTTCT GGAGTAGCCA GGGATACCCC AGTTGCTACT 
ACCCCAACAG GAGGAATCAC TAAGGAGATG AAGGAGGCTG ACAATCGCAG ACAGGCTGCA 
GCTGCTGCTG CTGCCTCTGC TGCCGCTGCT ACCACCTCTG CATCCACTGC CTCTGAAAGT 
GGAATGGATA AAGTTGTGTC TCAGCTCGCC ACTGTGGCCA AGAGTGTGCT TGGACCTGTC 
AAGCTCAATA TCCCAGGTAC TAAAGAGACC ATGGAACGAA CAAAGGACCA GCGTACCCAG 
GCAGATGCCA CCCACCCAAA GATAAAAGAA TGTCGCCTGG AGCACCCTGA TTCTCCTGAC 
CAATTGAGTC CATCTGCTGC TTCGGGCTCC CAGTCTCCAC GACTGACTGA ATCTCCATCT 
AAACAAACAT CATCCAAATA TGAGCCAGCC TCAACTTCTC AGAGACCTTT AGAGGTCCCA 
GGGACTTTCA AAAGACAAAC TTCTCCAATC CCTCAAGCTA GGGAAGAGCA TCAGGATAGG 
TCAGGAACTC TGCAAAGTGG CACCACATCA AGGCCTAGGT CTGAAGCACA ATCACAGACC 
CAGCAGGCAA AGCCCAGTTC CACTACGTCA CGAGATAAAT CCAACAAGAC AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327474.1
CDS	123..5699
Translation	MAGILTGKQLLPLSSSMHGGVSQLAWQQHTGEPNNLVRMRSQSFGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQATLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYTPPSHVLRPTTVMASPHTIRIGPAAPPTLGLFPSTVAPPSSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKYEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_021471799.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471799.1

ORF Insert Sequence:

ATGGCTGGCA TCCTCACTGG AAAGCAGCTT CTGCCTCTGT CCAGCAGTAT GCATGGAGGA 
GTGAGTCAGC TGGCCTGGCA GCAGCACACA GGGGAGCCCA ATAACTTGGT CCGCATGAGG 
AGTCAGTCCT TTGGCCAGTC TGCCCCGTCT CTCACTGCGG GACTGAAGGA GCTGAGTCTT 
CCTAGAAGAG GCAGTTTCTG TCGGACGAGC AACCGAAAAA GTTTGATAGT GACATCCAGC 
ACGTCTCCAA CGCTGCCTCG ACCCCATTCA CCACTGCACG GGCATCCAGG AAGCAGCCCA 
TTGGACAGCC CTCGCAATTT CTCCCCCAAC ACTGCAGCCC ACTTCTCTTT TGTACCAGCT 
CGAAGGACTG ATGGTCGTCG CTGGTCTCTC GCCTCTCTGC CGTCCTCTGG CTATGGAACA 
AACACCCCCA GTTCGACAGT CTCTTCATCA TGTTCATCTC AGGAGAAGCT GCACCAGTTG 
CCCTTCCAGC CCACTGCAGA CGAGCTGCAT TTCCTCACCA AACACTTCAG CTCGGAGAGC 
ATCACGGATG AGGATGGGAG ACGATCCCCA GCCATGCGTC CGCGCTCCCG CAGTCTCAGC 
CCTGGACGGT CACCAATATC ATTTGACCAT GAGATTGTCA TGATGAACCA TGTCTACAAG 
GAGCGTTTTC CTAAGGCCAC TGCTCAGATG GAGGTGCGTC TGGCCGATTT CATATCCTCC 
TCAGCTCCAG AAAAAGTCAT GCCTTTAGCT GATGGCGTCC TGAGCTTCAT CCACCACCAG 
GTTATCGAAC TGTCTCGTGA CTGTCTGGAC AAATCTCGTG AGGGTCTCAT CACCTCCCGC 
TACTTTTATG AGCTACAAGA GAACCTCGAA AAGCTACACC AGGATGCTCA TGAGCGTTCA 
GAAAGTGGGG AGGTGACATT TGTCACCCAG CTAGTTAAAA AGCTGATGAT AATCATCGCT 
CGGCCTGCCC GGCTGCTAGA ATGTTTGGAG TTTGACCCGG AGGAGTTCTA CCACCTGTTA 
GAGGCAGCTG AAGGTCATGC TAAAGAAGGT CAAGGCATCA AGTCTGACAT CCCTCGATAC 
ATCATCAGCC AGCTGGGCCT CACCAGGGAC CCCCTTGAGG AAATGGCCCA GCTGAGCAGC 
TATGACAGCG GTAACCCTGA AACACCAGAG ACAGAGACAG ATTCCACAGA TAGTCGAGGA 
GCAACCGTAC AGCCACTCCA ACCTCAGCCT CAACCGCAGC CCAAGACTAA AACACCTCGC 
GAGGAAGACT TCGAGACCAT TAAACTCATC AGTAATGGAG CCTATGGGGC TGTGTTTCTG 
GTGAGGCATA AAGAGACCCG TCAGCGATTT GCCATGAAGA AGATCAACAA GCAGAACCTG 
ATCCTAAGAA ACCAGATCCA GCAGGCCTTT GTGGAAAGAG ACATCTTGAC ATTTGCAGAA 
AACCCTTTTG TTGTCAGCAT GTTCTGCTCC TTTGAGACAA GGAGACATCT TTGCATGGTT 
ATGGAGTATG TGGAGGGTGG GGATTGTGCC ACTCTCCTGA AGCACATTGG AGCTCTGCCT 
GTGGACATGG CACGCATGTA CTTTGCTGAA ACCGTTCTTG CATTGGAATA CCTTCACAAC 
TATGGTATTG TGCACAGAGA CCTCAAACCT GACAATCTCC TGATTACCTC AATGGGACAC 
GTCAAACTGA CTGACTTTGG CCTGTCCAAG ATTGGTTTAA TGAGCTTAAC AACTAATCTG 
TATGAGGGCC ACATTGAAAA AGATACTCGA GAATTCTTGG ACAAACAGGT GTGTGGTACT 
CCTGAATACA TTGCTCCTGA GGTTATTCTG CGACAGGGTT ATGGAAAACC AGTTGACTGG 
TGGGCCATGG GAGTCATCTT GTATGAGTTT CTGGTGGGCT GTGCTCCTTT CTTTGGAGAT 
ACACCTGAAG AACTGTTTGG ACAAGTCATC AGTGATGAGA TAATCTGGCC AGAAGGTGAT 
GAAGCTTTGC CTCCGGATGC TCAAGATCTC ATCTCCAAAC TTCTTCGGCA AAACCCTCTA 
GAGCGTCTTG GGACAGGGAG TGCGTTTGAG GTAAAACAGC ACCGCTTCTT TACTGATCTG 
GACTGGAACA GCCTCCTGAG GCAGAAGGCC GAGTTCATTC CTCAGCTTGA GTCGGAGGAT 
GACACTAGCT ACTTTGACAC ACGCTCAGAT CGTTATCATC ATGTGGACTC AGAAGAAGAA 
GATGACACAA ATGATGACGA CCATTTGGAG ATCCGTCAGT TCTCCTCCTG TTCGCCACGA 
TTCAGCAAGG TGTATAGTAG TATGGAGCGT CTTTCCTTGC ATGAGGAGAA ACGCACCCCT 
CCTCCTACTA AGCGCAGTCT GAGTGAAGAG GGAGGAGAGC GGATCGACAG TCTAAGTGGC 
CTAAAGTCCA GAGACCGAAG CTGGTTGGTG GGCTCACCTG AAATTCTCAG AAAGCGTCTC 
TCTGTTTCCG AGTCTTCTCA CACCGAGAGT GACTCGAGTC CACCTCTGAC TGTTCGTCGC 
CGCTGCTGCT CGGCCATCAT TGAAATGCCT CGCTTCGCCA TATCCAGTGA AGAGGATAGC 
TGTGTGGGGA GCAGGAAGAC CCCTGTGAGA GGCCCTCGAG GGGATGATCT GCCTCAGGCC 
ATACCAGAGC TCCCTGTAGA GAGAGAGCTC AGGCTGGATG AATCGCCCAC CACGCCAGGA 
TCCACTTCCA GTCAAATCAG CCAGGCCACA CTCACACCTG GTAGTTCTGG TGATGTGTTA 
GATCGTGCTT CTCGCTATAG TGCTGAAGTT TCTGAAAACT CGACTCCAAG AGCCATCAGT 
GATTTGGCGG CTCGAAGAGC TCGCCACAGA CTGCTTTCAG GAGATACAGA TAAACACACA 
TCACGTCCAC TTAGCAAAGT CATCAAATCA GCTTCAGCAA CAACCCTGTC ACTCATGATC 
CCTGCTGATC ACCATGGTGC TTCTCCACTG GCCAGTCCCA TGTCGCCTCA CTCTCTCTCT 
TCGAACCCCT CATCACGTGA CTCCTCGCCC AGCCGTGACC TTTCCCCAGC TGTCTGTAGT 
GTTAAACCTG CCATAATCAT CCACCGAGCT GGAAAGAAAT ATGGTTTTAC TTTACGTGCA 
ATCCGGGTTT ACATGGGAGA CACAGACATA TATACTGTCC ATCACATGGT CTGGCATGTG 
GAGGATGGAG GACCTGCTCA TGAGGCTGGA CTGAGAGAGG GAGATCTCAT CACACATGTT 
AACGGAGAGC CTGTCCATGG ACTTGTGCAC ACAGAAGTTG TTGAGCTCAT TCTTAAGAGT 
GGCAGTAAGG TGTCCATCTC TGCTACACCC TTTGAAAATA CATCAATTAA GGTTGGTCCT 
GCTCGCAAAA CTAGCTACAA GTCCAAAATG GCCCGTCGCA ACAAAAGAAC CAAAACTAGA 
GAAGGACAAG ACAGTAAGAA GAGGAATTCC CTCTTCCGTA AGATCACCAA ACAGGCGACC 
CTTCTTCACA CCAGTCGCAG CCTGTCCTCT CTGAATCGCT CGGTCTCTTC TGGTGAGAGC 
GTCCCAGGCT CTCCTACTCA CATGTCGCCT CGTTCGCCCA CACAGGGCTG CCGTTCAACA 
CCTGACTCCG CCCACTCAGT TGGTGGAAAC TCATCCCAAA GTAGCTCGCC TAGCTCCAGT 
GTGCCAAACT CTCCTGCCAG TTCTGGCCAG ATCAGACCCA GCTCCTTGCA TGGTCTGGCC 
CCCAAGCTGC AGCGCCAGTA CCGCTCCCCC CGCCGGAAGT CTGCAGGCAA CATCCCTTTA 
TCCCCTCTGG CTCGTACACC ATCGCCCACT CCACAGAGCA CTTCTCCCCA GCGTTCCCCT 
TCTCCCCTGC CAAGCCATGG GCTCATCACT TCTTCAATAG GCCAGTCTTT TCCTGTCAAA 
CTGCACTCTT CACCTCCATT AGTAAGACAG ATATCTCGGC CAAAGAGTGC AGACCCTCCT 
CGCTCTCCTT TGCTGAAACG AGTCCAGTCC GCAGAGAAAC TCGCTGCCTC TCTTTCTTCT 
TCATCCTCCT CTCCTTCTCC CTCTTCAGCT GCTGATAAAA AGCTCCCAGT TGGTGCCAGC 
CGCAAACACA GTCTTGATAC CTCACATTCA GAGTTTAAAA AAGAGATGCT GCAGCGAGAC 
CCTAGTCTAC AGAGCCTGCA AGAGTCAGCC AGTGAGACTC TTATGGGAGG AAGAGTGTCT 
CCTGCAGAAA AGGGAAGCCT ACAGAAAAGT TCAGTGCGTA AACTGGGCAG GCAAGAAGGT 
CCGGAGTCTG GAACGGGAAC TCTGGGACTT GTTCCTGGAA AGAGTAAATT GAAAGACAAG 
CTGTCTGCCA TACGTGCTGA ACGCCGCGAA TCTCTACAAA AGCAGGATGC CATCCATGAA 
GTGGATTCAT CTGAAGATGA GACAGACGAA GGGTCGGAAG ACAGCCAAGA TGGGCGCAGA 
ACTTGCTATA CTCCTCCTAG CCATGTCTTG AGGCCTACAA CTGTAATGGC CTCCCCCCAT 
ACCATCAGAA TTGGGCCAGC GGCTCCACCA ACACTGGGAC TTTTTCCTTC AACAGTTGCA 
CCGCCAAGCT CACCTCAGCT CATCAGAGGA CTGCAGATAC AAAACCAAAG TACCACTCAA 
TCTCAGACAC AAACTTCAAC CTCACAACCA GCGCAAGCTG CAGTTAAACC CCCAGAACAG 
AAACCAATAA CCTCGGTTAA AGACTCAAGT TCAACGATAA TTGATGATTC GAAAAAACAG 
TCACAGGCAC AAGACATTCA GTCCAGACCT TCTCCACATC AACCCAGTCC TCTGTCTAGT 
ACCTTTTCCA CCCAAGGCAG CGCCTTTACC TCTGTTAGTA AAGACCCATT TGTCAAGCCC 
ACCACACCTC CATTGGATCC CCGGAGGACC CCAAAAATGT CTGAGCCAAG GTCAAAAACC 
AGTGGTAGAA CTGATTCTAG CACTACTTCT GGAGTAGCCA GGGATACCCC AGTTGCTACT 
ACCCCAACAG GAGGAATCAC TAAGGAGATG AAGGAGGCTG ACAATCGCAG ACAGGCTGCA 
GCTGCTGCTG CTGCCTCTGC TGCCGCTGCT ACCACCTCTG CATCCACTGC CTCTGAAAGT 
GGAATGGATA AAGTTGTGTC TCAGCTCGCC ACTGTGGCCA AGAGTGTGCT TGGACCTGTC 
AAGCTCAATA TCCCAGGTAC TAAAGAGACC ATGGAACGAA CAAAGGACCA GCGTACCCAG 
GCAGATGCCA CCCACCCAAA GATAAAAGAA TGTCGCCTGG AGCACCCTGA TTCTCCTGAC 
CAATTGAGTC CATCTGCTGC TTCGGGCTCC CAGTCTCCAC GACTGACTGA ATCTCCATCT 
AAACAAACAT CATCCAAATA TGAGCCAGCC TCAACTTCTC AGAGACCTTT AGAGGTCCCA 
GGGACTTTCA AAAGACAAAC TTCTCCAATC CCTCAAGCTA GGGAAGAGCA TCAGGATAGG 
TCAGGAACTC TGCAAAGTGG CACCACATCA AGGCCTAGGT CTGAAGCACA ATCACAGACC 
CAGCAGGCAA AGCCCAGTTC CACTACGTCA CGAGATAAAT CCAACAAGAC AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_021471800.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327475.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X1, mRNA.

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CloneID	ODa144728
Clone ID Related Accession (Same CDS sequence)	XM_021471800.1
Accession Version	XM_021471800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394654.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACT CCTTCTGGAC CGTTCTCTCC AATTTCTCCC TGCCGGAGAG AAGCATGCTC 
CGCCGATCCA AGAGCTGTCG GACGAGCAAC CGAAAAAGTT TGATAGTGAC ATCCAGCACG 
TCTCCAACGC TGCCTCGACC CCATTCACCA CTGCACGGGC ATCCAGGAAG CAGCCCATTG 
GACAGCCCTC GCAATTTCTC CCCCAACACT GCAGCCCACT TCTCTTTTGT ACCAGCTCGA 
AGCCATGGAC ACAGGGCTGA CAGGACTGAT GGTCGTCGCT GGTCTCTCGC CTCTCTGCCG 
TCCTCTGGCT ATGGAACAAA CACCCCCAGT TCGACAGTCT CTTCATCATG TTCATCTCAG 
GAGAAGCTGC ACCAGTTGCC CTTCCAGCCC ACTGCAGACG AGCTGCATTT CCTCACCAAA 
CACTTCAGCT CGGAGAGCAT CACGGATGAG GATGGGAGAC GATCCCCAGC CATGCGTCCG 
CGCTCCCGCA GTCTCAGCCC TGGACGGTCA CCAATATCAT TTGACCATGA GATTGTCATG 
ATGAACCATG TCTACAAGGA GCGTTTTCCT AAGGCCACTG CTCAGATGGA GGTGCGTCTG 
GCCGATTTCA TATCCTCCTC AGCTCCAGAA AAAGTCATGC CTTTAGCTGA TGGCGTCCTG 
AGCTTCATCC ACCACCAGGT TATCGAACTG TCTCGTGACT GTCTGGACAA ATCTCGTGAG 
GGTCTCATCA CCTCCCGCTA CTTTTATGAG CTACAAGAGA ACCTCGAAAA GCTACACCAG 
GATGCTCATG AGCGTTCAGA AAGTGGGGAG GTGACATTTG TCACCCAGCT AGTTAAAAAG 
CTGATGATAA TCATCGCTCG GCCTGCCCGG CTGCTAGAAT GTTTGGAGTT TGACCCGGAG 
GAGTTCTACC ACCTGTTAGA GGCAGCTGAA GGTCATGCTA AAGAAGGTCA AGGCATCAAG 
TCTGACATCC CTCGATACAT CATCAGCCAG CTGGGCCTCA CCAGGGACCC CCTTGAGGAA 
ATGGCCCAGC TGAGCAGCTA TGACAGCGGT AACCCTGAAA CACCAGAGAC AGAGACAGAT 
TCCACAGATA GTCGAGGAGC AACCGTACAG CCACTCCAAC CTCAGCCTCA ACCGCAGCCC 
AAGACTAAAA CACCTCGCGA GGAAGACTTC GAGACCATTA AACTCATCAG TAATGGAGCC 
TATGGGGCTG TGTTTCTGGT GAGGCATAAA GAGACCCGTC AGCGATTTGC CATGAAGAAG 
ATCAACAAGC AGAACCTGAT CCTAAGAAAC CAGATCCAGC AGGCCTTTGT GGAAAGAGAC 
ATCTTGACAT TTGCAGAAAA CCCTTTTGTT GTCAGCATGT TCTGCTCCTT TGAGACAAGG 
AGACATCTTT GCATGGTTAT GGAGTATGTG GAGGGTGGGG ATTGTGCCAC TCTCCTGAAG 
CACATTGGAG CTCTGCCTGT GGACATGGCA CGCATGTACT TTGCTGAAAC CGTTCTTGCA 
TTGGAATACC TTCACAACTA TGGTATTGTG CACAGAGACC TCAAACCTGA CAATCTCCTG 
ATTACCTCAA TGGGACACGT CAAACTGACT GACTTTGGCC TGTCCAAGAT TGGTTTAATG 
AGCTTAACAA CTAATCTGTA TGAGGGCCAC ATTGAAAAAG ATACTCGAGA ATTCTTGGAC 
AAACAGGTGT GTGGTACTCC TGAATACATT GCTCCTGAGG TTATTCTGCG ACAGGGTTAT 
GGAAAACCAG TTGACTGGTG GGCCATGGGA GTCATCTTGT ATGAGTTTCT GGTGGGCTGT 
GCTCCTTTCT TTGGAGATAC ACCTGAAGAA CTGTTTGGAC AAGTCATCAG TGATGAGATA 
ATCTGGCCAG AAGGTGATGA AGCTTTGCCT CCGGATGCTC AAGATCTCAT CTCCAAACTT 
CTTCGGCAAA ACCCTCTAGA GCGTCTTGGG ACAGGGAGTG CGTTTGAGGT AAAACAGCAC 
CGCTTCTTTA CTGATCTGGA CTGGAACAGC CTCCTGAGGC AGAAGGCCGA GTTCATTCCT 
CAGCTTGAGT CGGAGGATGA CACTAGCTAC TTTGACACAC GCTCAGATCG TTATCATCAT 
GTGGACTCAG AAGAAGAAGA TGACACAAAT GATGACGACC ATTTGGAGAT CCGTCAGTTC 
TCCTCCTGTT CGCCACGATT CAGCAAGGTG TATAGTAGTA TGGAGCGTCT TTCCTTGCAT 
GAGGAGAAAC GCACCCCTCC TCCTACTAAG CGCAGTCTGA GTGAAGAGGG AGGAGAGCGG 
ATCGACAGTC TAAGTGGCCT AAAGTCCAGA GACCGAAGCT GGTTGGTGGG CTCACCTGAA 
ATTCTCAGAA AGCGTCTCTC TGTTTCCGAG TCTTCTCACA CCGAGAGTGA CTCGAGTCCA 
CCTCTGACTG TTCGTCGCCG CTGCTGCTCG GCCATCATTG AAATGCCTCG CTTCGCCATA 
TCCAGTGAAG AGGATAGCTG TGTGGGGAGC AGGAAGACCC CTGTGAGAGG CCCTCGAGGG 
GATGATCTGC CTCAGGCCAT ACCAGAGCTC CCTGTAGAGA GAGAGCTCAG GCTGGATGAA 
TCGCCCACCA CGCCAGGATC CACTTCCAGT CAAATCAGCC AGGCCACACT CACACCTGGT 
AGTTCTGGTG ATGTGTTAGA TCGTGCTTCT CGCTATAGTG CTGAAGTTTC TGAAAACTCG 
ACTCCAAGAG CCATCAGTGA TTTGGCGGCT CGAAGAGCTC GCCACAGACT GCTTTCAGGA 
GATACAGATA AACACACATC ACGTCCACTT AGCAAAGTCA TCAAATCAGC TTCAGCAACA 
ACCCTGTCAC TCATGATCCC TGCTGATCAC CATGGTGCTT CTCCACTGGC CAGTCCCATG 
TCGCCTCACT CTCTCTCTTC GAACCCCTCA TCACGTGACT CCTCGCCCAG CCGTGACCTT 
TCCCCAGCTG TCTGTAGTGT TAAACCTGCC ATAATCATCC ACCGAGCTGG AAAGAAATAT 
GGTTTTACTT TACGTGCAAT CCGGGTTTAC ATGGGAGACA CAGACATATA TACTGTCCAT 
CACATGGTCT GGCATGTGGA GGATGGAGGA CCTGCTCATG AGGCTGGACT GAGAGAGGGA 
GATCTCATCA CACATGTTAA CGGAGAGCCT GTCCATGGAC TTGTGCACAC AGAAGTTGTT 
GAGCTCATTC TTAAGAGTGG CAGTAAGGTG TCCATCTCTG CTACACCCTT TGAAAATACA 
TCAATTAAGG TTGGTCCTGC TCGCAAAACT AGCTACAAGT CCAAAATGGC CCGTCGCAAC 
AAAAGAACCA AAACTAGAGA AGGACAAGAC AGTAAGAAGA GGAATTCCCT CTTCCGTAAG 
ATCACCAAAC AGGCGACCCT TCTTCACACC AGTCGCAGCC TGTCCTCTCT GAATCGCTCG 
GTCTCTTCTG GTGAGAGCGT CCCAGGCTCT CCTACTCACA TGTCGCCTCG TTCGCCCACA 
CAGGGCTGCC GTTCAACACC TGACTCCGCC CACTCAGTTG GTGGAAACTC ATCCCAAAGT 
AGCTCGCCTA GCTCCAGTGT GCCAAACTCT CCTGCCAGTT CTGGCCAGAT CAGACCCAGC 
TCCTTGCATG GTCTGGCCCC CAAGCTGCAG CGCCAGTACC GCTCCCCCCG CCGGAAGTCT 
GCAGGCAACA TCCCTTTATC CCCTCTGGCT CGTACACCAT CGCCCACTCC ACAGAGCACT 
TCTCCCCAGC GTTCCCCTTC TCCCCTGCCA AGCCATGGGC TCATCACTTC TTCAATAGGC 
CAGTCTTTTC CTGTCAAACT GCACTCTTCA CCTCCATTAG TAAGACAGAT ATCTCGGCCA 
AAGAGTGCAG ACCCTCCTCG CTCTCCTTTG CTGAAACGAG TCCAGTCCGC AGAGAAACTC 
GCTGCCTCTC TTTCTTCTTC ATCCTCCTCT CCTTCTCCCT CTTCAGCTGC TGATAAAAAG 
CTCCCAGTTG GTGCCAGCCG CAAACACAGT CTTGATACCT CACATTCAGA GTTTAAAAAA 
GAGATGCTGC AGCGAGACCC TAGTCTACAG AGCCTGCAAG AGTCAGCCAG TGAGACTCTT 
ATGGGAGGAA GAGTGTCTCC TGCAGAAAAG GGAAGCCTAC AGAAAAGTTC AGTGCGTAAA 
CTGGGCAGGC AAGAAGGTCC GGAGTCTGGA ACGGGAACTC TGGGACTTGT TCCTGGAAAG 
AGTAAATTGA AAGACAAGCT GTCTGCCATA CGTGCTGAAC GCCGCGAATC TCTACAAAAG 
CAGGATGCCA TCCATGAAGT GGATTCATCT GAAGATGAGA CAGACGAAGG GTCGGAAGAC 
AGCCAAGATG GGCGCAGAAC TTGCTATACT CCTCCTAGCC ATGTCTTGAG GCCTACAACT 
GTAATGGCCT CCCCCCATAC CATCAGAATT GGGCCAGCGG CTCCACCAAC ACTGGGACTT 
TTTCCTTCAA CAGTTGCACC GCCAAGCTCA CCTCAGCTCA TCAGAGGACT GCAGATACAA 
AACCAAAGTA CCACTCAATC TCAGACACAA ACTTCAACCT CACAACCAGC GCAAGCTGCA 
GTTAAACCCC CAGAACAGAA ACCAATAACC TCGGTTAAAG ACTCAAGTTC AACGATAATT 
GATGATTCGA AAAAACAGTC ACAGGCACAA GACATTCAGT CCAGACCTTC TCCACATCAA 
CCCAGTCCTC TGTCTAGTAC CTTTTCCACC CAAGGCAGCG CCTTTACCTC TGTTAGTAAA 
GACCCATTTG TCAAGCCCAC CACACCTCCA TTGGATCCCC GGAGGACCCC AAAAATGTCT 
GAGCCAAGGT CAAAAACCAG TGGTAGAACT GATTCTAGCA CTACTTCTGG AGTAGCCAGG 
GATACCCCAG TTGCTACTAC CCCAACAGGA GGAATCACTA AGGAGATGAA GGAGGCTGAC 
AATCGCAGAC AGGCTGCAGC TGCTGCTGCT GCCTCTGCTG CCGCTGCTAC CACCTCTGCA 
TCCACTGCCT CTGAAAGTGG AATGGATAAA GTTGTGTCTC AGCTCGCCAC TGTGGCCAAG 
AGTGTGCTTG GACCTGTCAA GCTCAATATC CCAGGTACTA AAGAGACCAT GGAACGAACA 
AAGGACCAGC GTACCCAGGC AGATGCCACC CACCCAAAGA TAAAAGAATG TCGCCTGGAG 
CACCCTGATT CTCCTGACCA ATTGAGTCCA TCTGCTGCTT CGGGCTCCCA GTCTCCACGA 
CTGACTGAAT CTCCATCTAA ACAAACATCA TCCAAATATG AGCCAGCCTC AACTTCTCAG 
AGACCTTTAG AGGTCCCAGG GACTTTCAAA AGACAAACTT CTCCAATCCC TCAAGCTAGG 
GAAGAGCATC AGGATAGGTC AGGAACTCTG CAAAGTGGCA CCACATCAAG GCCTAGGTCT 
GAAGCACAAT CACAGACCCA GCAGGCAAAG CCCAGTTCCA CTACGTCACG AGATAAATCC 
AACAAGACAA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327475.1
CDS	175..5649
Translation	MSDSFWTVLSNFSLPERSMLRRSKSCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQATLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYTPPSHVLRPTTVMASPHTIRIGPAAPPTLGLFPSTVAPPSSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKYEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_021471800.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471800.1

ORF Insert Sequence:

ATGTCTGACT CCTTCTGGAC CGTTCTCTCC AATTTCTCCC TGCCGGAGAG AAGCATGCTC 
CGCCGATCCA AGAGCTGTCG GACGAGCAAC CGAAAAAGTT TGATAGTGAC ATCCAGCACG 
TCTCCAACGC TGCCTCGACC CCATTCACCA CTGCACGGGC ATCCAGGAAG CAGCCCATTG 
GACAGCCCTC GCAATTTCTC CCCCAACACT GCAGCCCACT TCTCTTTTGT ACCAGCTCGA 
AGCCATGGAC ACAGGGCTGA CAGGACTGAT GGTCGTCGCT GGTCTCTCGC CTCTCTGCCG 
TCCTCTGGCT ATGGAACAAA CACCCCCAGT TCGACAGTCT CTTCATCATG TTCATCTCAG 
GAGAAGCTGC ACCAGTTGCC CTTCCAGCCC ACTGCAGACG AGCTGCATTT CCTCACCAAA 
CACTTCAGCT CGGAGAGCAT CACGGATGAG GATGGGAGAC GATCCCCAGC CATGCGTCCG 
CGCTCCCGCA GTCTCAGCCC TGGACGGTCA CCAATATCAT TTGACCATGA GATTGTCATG 
ATGAACCATG TCTACAAGGA GCGTTTTCCT AAGGCCACTG CTCAGATGGA GGTGCGTCTG 
GCCGATTTCA TATCCTCCTC AGCTCCAGAA AAAGTCATGC CTTTAGCTGA TGGCGTCCTG 
AGCTTCATCC ACCACCAGGT TATCGAACTG TCTCGTGACT GTCTGGACAA ATCTCGTGAG 
GGTCTCATCA CCTCCCGCTA CTTTTATGAG CTACAAGAGA ACCTCGAAAA GCTACACCAG 
GATGCTCATG AGCGTTCAGA AAGTGGGGAG GTGACATTTG TCACCCAGCT AGTTAAAAAG 
CTGATGATAA TCATCGCTCG GCCTGCCCGG CTGCTAGAAT GTTTGGAGTT TGACCCGGAG 
GAGTTCTACC ACCTGTTAGA GGCAGCTGAA GGTCATGCTA AAGAAGGTCA AGGCATCAAG 
TCTGACATCC CTCGATACAT CATCAGCCAG CTGGGCCTCA CCAGGGACCC CCTTGAGGAA 
ATGGCCCAGC TGAGCAGCTA TGACAGCGGT AACCCTGAAA CACCAGAGAC AGAGACAGAT 
TCCACAGATA GTCGAGGAGC AACCGTACAG CCACTCCAAC CTCAGCCTCA ACCGCAGCCC 
AAGACTAAAA CACCTCGCGA GGAAGACTTC GAGACCATTA AACTCATCAG TAATGGAGCC 
TATGGGGCTG TGTTTCTGGT GAGGCATAAA GAGACCCGTC AGCGATTTGC CATGAAGAAG 
ATCAACAAGC AGAACCTGAT CCTAAGAAAC CAGATCCAGC AGGCCTTTGT GGAAAGAGAC 
ATCTTGACAT TTGCAGAAAA CCCTTTTGTT GTCAGCATGT TCTGCTCCTT TGAGACAAGG 
AGACATCTTT GCATGGTTAT GGAGTATGTG GAGGGTGGGG ATTGTGCCAC TCTCCTGAAG 
CACATTGGAG CTCTGCCTGT GGACATGGCA CGCATGTACT TTGCTGAAAC CGTTCTTGCA 
TTGGAATACC TTCACAACTA TGGTATTGTG CACAGAGACC TCAAACCTGA CAATCTCCTG 
ATTACCTCAA TGGGACACGT CAAACTGACT GACTTTGGCC TGTCCAAGAT TGGTTTAATG 
AGCTTAACAA CTAATCTGTA TGAGGGCCAC ATTGAAAAAG ATACTCGAGA ATTCTTGGAC 
AAACAGGTGT GTGGTACTCC TGAATACATT GCTCCTGAGG TTATTCTGCG ACAGGGTTAT 
GGAAAACCAG TTGACTGGTG GGCCATGGGA GTCATCTTGT ATGAGTTTCT GGTGGGCTGT 
GCTCCTTTCT TTGGAGATAC ACCTGAAGAA CTGTTTGGAC AAGTCATCAG TGATGAGATA 
ATCTGGCCAG AAGGTGATGA AGCTTTGCCT CCGGATGCTC AAGATCTCAT CTCCAAACTT 
CTTCGGCAAA ACCCTCTAGA GCGTCTTGGG ACAGGGAGTG CGTTTGAGGT AAAACAGCAC 
CGCTTCTTTA CTGATCTGGA CTGGAACAGC CTCCTGAGGC AGAAGGCCGA GTTCATTCCT 
CAGCTTGAGT CGGAGGATGA CACTAGCTAC TTTGACACAC GCTCAGATCG TTATCATCAT 
GTGGACTCAG AAGAAGAAGA TGACACAAAT GATGACGACC ATTTGGAGAT CCGTCAGTTC 
TCCTCCTGTT CGCCACGATT CAGCAAGGTG TATAGTAGTA TGGAGCGTCT TTCCTTGCAT 
GAGGAGAAAC GCACCCCTCC TCCTACTAAG CGCAGTCTGA GTGAAGAGGG AGGAGAGCGG 
ATCGACAGTC TAAGTGGCCT AAAGTCCAGA GACCGAAGCT GGTTGGTGGG CTCACCTGAA 
ATTCTCAGAA AGCGTCTCTC TGTTTCCGAG TCTTCTCACA CCGAGAGTGA CTCGAGTCCA 
CCTCTGACTG TTCGTCGCCG CTGCTGCTCG GCCATCATTG AAATGCCTCG CTTCGCCATA 
TCCAGTGAAG AGGATAGCTG TGTGGGGAGC AGGAAGACCC CTGTGAGAGG CCCTCGAGGG 
GATGATCTGC CTCAGGCCAT ACCAGAGCTC CCTGTAGAGA GAGAGCTCAG GCTGGATGAA 
TCGCCCACCA CGCCAGGATC CACTTCCAGT CAAATCAGCC AGGCCACACT CACACCTGGT 
AGTTCTGGTG ATGTGTTAGA TCGTGCTTCT CGCTATAGTG CTGAAGTTTC TGAAAACTCG 
ACTCCAAGAG CCATCAGTGA TTTGGCGGCT CGAAGAGCTC GCCACAGACT GCTTTCAGGA 
GATACAGATA AACACACATC ACGTCCACTT AGCAAAGTCA TCAAATCAGC TTCAGCAACA 
ACCCTGTCAC TCATGATCCC TGCTGATCAC CATGGTGCTT CTCCACTGGC CAGTCCCATG 
TCGCCTCACT CTCTCTCTTC GAACCCCTCA TCACGTGACT CCTCGCCCAG CCGTGACCTT 
TCCCCAGCTG TCTGTAGTGT TAAACCTGCC ATAATCATCC ACCGAGCTGG AAAGAAATAT 
GGTTTTACTT TACGTGCAAT CCGGGTTTAC ATGGGAGACA CAGACATATA TACTGTCCAT 
CACATGGTCT GGCATGTGGA GGATGGAGGA CCTGCTCATG AGGCTGGACT GAGAGAGGGA 
GATCTCATCA CACATGTTAA CGGAGAGCCT GTCCATGGAC TTGTGCACAC AGAAGTTGTT 
GAGCTCATTC TTAAGAGTGG CAGTAAGGTG TCCATCTCTG CTACACCCTT TGAAAATACA 
TCAATTAAGG TTGGTCCTGC TCGCAAAACT AGCTACAAGT CCAAAATGGC CCGTCGCAAC 
AAAAGAACCA AAACTAGAGA AGGACAAGAC AGTAAGAAGA GGAATTCCCT CTTCCGTAAG 
ATCACCAAAC AGGCGACCCT TCTTCACACC AGTCGCAGCC TGTCCTCTCT GAATCGCTCG 
GTCTCTTCTG GTGAGAGCGT CCCAGGCTCT CCTACTCACA TGTCGCCTCG TTCGCCCACA 
CAGGGCTGCC GTTCAACACC TGACTCCGCC CACTCAGTTG GTGGAAACTC ATCCCAAAGT 
AGCTCGCCTA GCTCCAGTGT GCCAAACTCT CCTGCCAGTT CTGGCCAGAT CAGACCCAGC 
TCCTTGCATG GTCTGGCCCC CAAGCTGCAG CGCCAGTACC GCTCCCCCCG CCGGAAGTCT 
GCAGGCAACA TCCCTTTATC CCCTCTGGCT CGTACACCAT CGCCCACTCC ACAGAGCACT 
TCTCCCCAGC GTTCCCCTTC TCCCCTGCCA AGCCATGGGC TCATCACTTC TTCAATAGGC 
CAGTCTTTTC CTGTCAAACT GCACTCTTCA CCTCCATTAG TAAGACAGAT ATCTCGGCCA 
AAGAGTGCAG ACCCTCCTCG CTCTCCTTTG CTGAAACGAG TCCAGTCCGC AGAGAAACTC 
GCTGCCTCTC TTTCTTCTTC ATCCTCCTCT CCTTCTCCCT CTTCAGCTGC TGATAAAAAG 
CTCCCAGTTG GTGCCAGCCG CAAACACAGT CTTGATACCT CACATTCAGA GTTTAAAAAA 
GAGATGCTGC AGCGAGACCC TAGTCTACAG AGCCTGCAAG AGTCAGCCAG TGAGACTCTT 
ATGGGAGGAA GAGTGTCTCC TGCAGAAAAG GGAAGCCTAC AGAAAAGTTC AGTGCGTAAA 
CTGGGCAGGC AAGAAGGTCC GGAGTCTGGA ACGGGAACTC TGGGACTTGT TCCTGGAAAG 
AGTAAATTGA AAGACAAGCT GTCTGCCATA CGTGCTGAAC GCCGCGAATC TCTACAAAAG 
CAGGATGCCA TCCATGAAGT GGATTCATCT GAAGATGAGA CAGACGAAGG GTCGGAAGAC 
AGCCAAGATG GGCGCAGAAC TTGCTATACT CCTCCTAGCC ATGTCTTGAG GCCTACAACT 
GTAATGGCCT CCCCCCATAC CATCAGAATT GGGCCAGCGG CTCCACCAAC ACTGGGACTT 
TTTCCTTCAA CAGTTGCACC GCCAAGCTCA CCTCAGCTCA TCAGAGGACT GCAGATACAA 
AACCAAAGTA CCACTCAATC TCAGACACAA ACTTCAACCT CACAACCAGC GCAAGCTGCA 
GTTAAACCCC CAGAACAGAA ACCAATAACC TCGGTTAAAG ACTCAAGTTC AACGATAATT 
GATGATTCGA AAAAACAGTC ACAGGCACAA GACATTCAGT CCAGACCTTC TCCACATCAA 
CCCAGTCCTC TGTCTAGTAC CTTTTCCACC CAAGGCAGCG CCTTTACCTC TGTTAGTAAA 
GACCCATTTG TCAAGCCCAC CACACCTCCA TTGGATCCCC GGAGGACCCC AAAAATGTCT 
GAGCCAAGGT CAAAAACCAG TGGTAGAACT GATTCTAGCA CTACTTCTGG AGTAGCCAGG 
GATACCCCAG TTGCTACTAC CCCAACAGGA GGAATCACTA AGGAGATGAA GGAGGCTGAC 
AATCGCAGAC AGGCTGCAGC TGCTGCTGCT GCCTCTGCTG CCGCTGCTAC CACCTCTGCA 
TCCACTGCCT CTGAAAGTGG AATGGATAAA GTTGTGTCTC AGCTCGCCAC TGTGGCCAAG 
AGTGTGCTTG GACCTGTCAA GCTCAATATC CCAGGTACTA AAGAGACCAT GGAACGAACA 
AAGGACCAGC GTACCCAGGC AGATGCCACC CACCCAAAGA TAAAAGAATG TCGCCTGGAG 
CACCCTGATT CTCCTGACCA ATTGAGTCCA TCTGCTGCTT CGGGCTCCCA GTCTCCACGA 
CTGACTGAAT CTCCATCTAA ACAAACATCA TCCAAATATG AGCCAGCCTC AACTTCTCAG 
AGACCTTTAG AGGTCCCAGG GACTTTCAAA AGACAAACTT CTCCAATCCC TCAAGCTAGG 
GAAGAGCATC AGGATAGGTC AGGAACTCTG CAAAGTGGCA CCACATCAAG GCCTAGGTCT 
GAAGCACAAT CACAGACCCA GCAGGCAAAG CCCAGTTCCA CTACGTCACG AGATAAATCC 
AACAAGACAA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_021471801.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327476.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X2, mRNA.

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CloneID	ODa144729
Clone ID Related Accession (Same CDS sequence)	XM_021471801.1
Accession Version	XM_021471801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394654.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACT CCAGTATATT AAGGCGAAGA GGCCTTCAGA AGGAGCTGAG TCTTCCTAGA 
AGAGGCAGTT TCTGTCGGAC GAGCAACCGA AAAAGTTTGA TAGTGACATC CAGCACGTCT 
CCAACGCTGC CTCGACCCCA TTCACCACTG CACGGGCATC CAGGAAGCAG CCCATTGGAC 
AGCCCTCGCA ATTTCTCCCC CAACACTGCA GCCCACTTCT CTTTTGTACC AGCTCGAAGC 
CATGGACACA GGGCTGACAG GACTGATGGT CGTCGCTGGT CTCTCGCCTC TCTGCCGTCC 
TCTGGCTATG GAACAAACAC CCCCAGTTCG ACAGTCTCTT CATCATGTTC ATCTCAGGAG 
AAGCTGCACC AGTTGCCCTT CCAGCCCACT GCAGACGAGC TGCATTTCCT CACCAAACAC 
TTCAGCTCGG AGAGCATCAC GGATGAGGAT GGGAGACGAT CCCCAGCCAT GCGTCCGCGC 
TCCCGCAGTC TCAGCCCTGG ACGGTCACCA ATATCATTTG ACCATGAGAT TGTCATGATG 
AACCATGTCT ACAAGGAGCG TTTTCCTAAG GCCACTGCTC AGATGGAGGT GCGTCTGGCC 
GATTTCATAT CCTCCTCAGC TCCAGAAAAA GTCATGCCTT TAGCTGATGG CGTCCTGAGC 
TTCATCCACC ACCAGGTTAT CGAACTGTCT CGTGACTGTC TGGACAAATC TCGTGAGGGT 
CTCATCACCT CCCGCTACTT TTATGAGCTA CAAGAGAACC TCGAAAAGCT ACACCAGGAT 
GCTCATGAGC GTTCAGAAAG TGGGGAGGTG ACATTTGTCA CCCAGCTAGT TAAAAAGCTG 
ATGATAATCA TCGCTCGGCC TGCCCGGCTG CTAGAATGTT TGGAGTTTGA CCCGGAGGAG 
TTCTACCACC TGTTAGAGGC AGCTGAAGGT CATGCTAAAG AAGGTCAAGG CATCAAGTCT 
GACATCCCTC GATACATCAT CAGCCAGCTG GGCCTCACCA GGGACCCCCT TGAGGAAATG 
GCCCAGCTGA GCAGCTATGA CAGCGGTAAC CCTGAAACAC CAGAGACAGA GACAGATTCC 
ACAGATAGTC GAGGAGCAAC CGTACAGCCA CTCCAACCTC AGCCTCAACC GCAGCCCAAG 
ACTAAAACAC CTCGCGAGGA AGACTTCGAG ACCATTAAAC TCATCAGTAA TGGAGCCTAT 
GGGGCTGTGT TTCTGGTGAG GCATAAAGAG ACCCGTCAGC GATTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTGATCCT AAGAAACCAG ATCCAGCAGG CCTTTGTGGA AAGAGACATC 
TTGACATTTG CAGAAAACCC TTTTGTTGTC AGCATGTTCT GCTCCTTTGA GACAAGGAGA 
CATCTTTGCA TGGTTATGGA GTATGTGGAG GGTGGGGATT GTGCCACTCT CCTGAAGCAC 
ATTGGAGCTC TGCCTGTGGA CATGGCACGC ATGTACTTTG CTGAAACCGT TCTTGCATTG 
GAATACCTTC ACAACTATGG TATTGTGCAC AGAGACCTCA AACCTGACAA TCTCCTGATT 
ACCTCAATGG GACACGTCAA ACTGACTGAC TTTGGCCTGT CCAAGATTGG TTTAATGAGC 
TTAACAACTA ATCTGTATGA GGGCCACATT GAAAAAGATA CTCGAGAATT CTTGGACAAA 
CAGGTGTGTG GTACTCCTGA ATACATTGCT CCTGAGGTTA TTCTGCGACA GGGTTATGGA 
AAACCAGTTG ACTGGTGGGC CATGGGAGTC ATCTTGTATG AGTTTCTGGT GGGCTGTGCT 
CCTTTCTTTG GAGATACACC TGAAGAACTG TTTGGACAAG TCATCAGTGA TGAGATAATC 
TGGCCAGAAG GTGATGAAGC TTTGCCTCCG GATGCTCAAG ATCTCATCTC CAAACTTCTT 
CGGCAAAACC CTCTAGAGCG TCTTGGGACA GGGAGTGCGT TTGAGGTAAA ACAGCACCGC 
TTCTTTACTG ATCTGGACTG GAACAGCCTC CTGAGGCAGA AGGCCGAGTT CATTCCTCAG 
CTTGAGTCGG AGGATGACAC TAGCTACTTT GACACACGCT CAGATCGTTA TCATCATGTG 
GACTCAGAAG AAGAAGATGA CACAAATGAT GACGACCATT TGGAGATCCG TCAGTTCTCC 
TCCTGTTCGC CACGATTCAG CAAGGTGTAT AGTAGTATGG AGCGTCTTTC CTTGCATGAG 
GAGAAACGCA CCCCTCCTCC TACTAAGCGC AGTCTGAGTG AAGAGGGAGG AGAGCGGATC 
GACAGTCTAA GTGGCCTAAA GTCCAGAGAC CGAAGCTGGT TGGTGGGCTC ACCTGAAATT 
CTCAGAAAGC GTCTCTCTGT TTCCGAGTCT TCTCACACCG AGAGTGACTC GAGTCCACCT 
CTGACTGTTC GTCGCCGCTG CTGCTCGGCC ATCATTGAAA TGCCTCGCTT CGCCATATCC 
AGTGAAGAGG ATAGCTGTGT GGGGAGCAGG AAGACCCCTG TGAGAGGCCC TCGAGGGGAT 
GATCTGCCTC AGGCCATACC AGAGCTCCCT GTAGAGAGAG AGCTCAGGCT GGATGAATCG 
CCCACCACGC CAGGATCCAC TTCCAGTCAA ATCAGCCAGG CCACACTCAC ACCTGGTAGT 
TCTGGTGATG TGTTAGATCG TGCTTCTCGC TATAGTGCTG AAGTTTCTGA AAACTCGACT 
CCAAGAGCCA TCAGTGATTT GGCGGCTCGA AGAGCTCGCC ACAGACTGCT TTCAGGAGAT 
ACAGATAAAC ACACATCACG TCCACTTAGC AAAGTCATCA AATCAGCTTC AGCAACAACC 
CTGTCACTCA TGATCCCTGC TGATCACCAT GGTGCTTCTC CACTGGCCAG TCCCATGTCG 
CCTCACTCTC TCTCTTCGAA CCCCTCATCA CGTGACTCCT CGCCCAGCCG TGACCTTTCC 
CCAGCTGTCT GTAGTGTTAA ACCTGCCATA ATCATCCACC GAGCTGGAAA GAAATATGGT 
TTTACTTTAC GTGCAATCCG GGTTTACATG GGAGACACAG ACATATATAC TGTCCATCAC 
ATGGTCTGGC ATGTGGAGGA TGGAGGACCT GCTCATGAGG CTGGACTGAG AGAGGGAGAT 
CTCATCACAC ATGTTAACGG AGAGCCTGTC CATGGACTTG TGCACACAGA AGTTGTTGAG 
CTCATTCTTA AGAGTGGCAG TAAGGTGTCC ATCTCTGCTA CACCCTTTGA AAATACATCA 
ATTAAGGTTG GTCCTGCTCG CAAAACTAGC TACAAGTCCA AAATGGCCCG TCGCAACAAA 
AGAACCAAAA CTAGAGAAGG ACAAGACAGT AAGAAGAGGA ATTCCCTCTT CCGTAAGATC 
ACCAAACAGG CGACCCTTCT TCACACCAGT CGCAGCCTGT CCTCTCTGAA TCGCTCGGTC 
TCTTCTGGTG AGAGCGTCCC AGGCTCTCCT ACTCACATGT CGCCTCGTTC GCCCACACAG 
GGCTGCCGTT CAACACCTGA CTCCGCCCAC TCAGTTGGTG GAAACTCATC CCAAAGTAGC 
TCGCCTAGCT CCAGTGTGCC AAACTCTCCT GCCAGTTCTG GCCAGATCAG ACCCAGCTCC 
TTGCATGGTC TGGCCCCCAA GCTGCAGCGC CAGTACCGCT CCCCCCGCCG GAAGTCTGCA 
GGCAACATCC CTTTATCCCC TCTGGCTCGT ACACCATCGC CCACTCCACA GAGCACTTCT 
CCCCAGCGTT CCCCTTCTCC CCTGCCAAGC CATGGGCTCA TCACTTCTTC AATAGGCCAG 
TCTTTTCCTG TCAAACTGCA CTCTTCACCT CCATTAGTAA GACAGATATC TCGGCCAAAG 
AGTGCAGACC CTCCTCGCTC TCCTTTGCTG AAACGAGTCC AGTCCGCAGA GAAACTCGCT 
GCCTCTCTTT CTTCTTCATC CTCCTCTCCT TCTCCCTCTT CAGCTGCTGA TAAAAAGCTC 
CCAGTTGGTG CCAGCCGCAA ACACAGTCTT GATACCTCAC ATTCAGAGTT TAAAAAAGAG 
ATGCTGCAGC GAGACCCTAG TCTACAGAGC CTGCAAGAGT CAGCCAGTGA GACTCTTATG 
GGAGGAAGAG TGTCTCCTGC AGAAAAGGGA AGCCTACAGA AAAGTTCAGT GCGTAAACTG 
GGCAGGCAAG AAGGTCCGGA GTCTGGAACG GGAACTCTGG GACTTGTTCC TGGAAAGAGT 
AAATTGAAAG ACAAGCTGTC TGCCATACGT GCTGAACGCC GCGAATCTCT ACAAAAGCAG 
GATGCCATCC ATGAAGTGGA TTCATCTGAA GATGAGACAG ACGAAGGGTC GGAAGACAGC 
CAAGATGGGC GCAGAACTTG CTATACTCCT CCTAGCCATG TCTTGAGGCC TACAACTGTA 
ATGGCCTCCC CCCATACCAT CAGAATTGGG CCAGCGGCTC CACCAACACT GGGACTTTTT 
CCTTCAACAG TTGCACCGCC AAGCTCACCT CAGCTCATCA GAGGACTGCA GATACAAAAC 
CAAAGTACCA CTCAATCTCA GACACAAACT TCAACCTCAC AACCAGCGCA AGCTGCAGTT 
AAACCCCCAG AACAGAAACC AATAACCTCG GTTAAAGACT CAAGTTCAAC GATAATTGAT 
GATTCGAAAA AACAGTCACA GGCACAAGAC ATTCAGTCCA GACCTTCTCC ACATCAACCC 
AGTCCTCTGT CTAGTACCTT TTCCACCCAA GGCAGCGCCT TTACCTCTGT TAGTAAAGAC 
CCATTTGTCA AGCCCACCAC ACCTCCATTG GATCCCCGGA GGACCCCAAA AATGTCTGAG 
CCAAGGTCAA AAACCAGTGG TAGAACTGAT TCTAGCACTA CTTCTGGAGT AGCCAGGGAT 
ACCCCAGTTG CTACTACCCC AACAGGAGGA ATCACTAAGG AGATGAAGGA GGCTGACAAT 
CGCAGACAGG CTGCAGCTGC TGCTGCTGCC TCTGCTGCCG CTGCTACCAC CTCTGCATCC 
ACTGCCTCTG AAAGTGGAAT GGATAAAGTT GTGTCTCAGC TCGCCACTGT GGCCAAGAGT 
GTGCTTGGAC CTGTCAAGCT CAATATCCCA GGTACTAAAG AGACCATGGA ACGAACAAAG 
GACCAGCGTA CCCAGGCAGA TGCCACCCAC CCAAAGATAA AAGAATGTCG CCTGGAGCAC 
CCTGATTCTC CTGACCAATT GAGTCCATCT GCTGCTTCGG GCTCCCAGTC TCCACGACTG 
ACTGAATCTC CATCTAAACA AACATCATCC AAATATGAGC CAGCCTCAAC TTCTCAGAGA 
CCTTTAGAGG TCCCAGGGAC TTTCAAAAGA CAAACTTCTC CAATCCCTCA AGCTAGGGAA 
GAGCATCAGG ATAGGTCAGG AACTCTGCAA AGTGGCACCA CATCAAGGCC TAGGTCTGAA 
GCACAATCAC AGACCCAGCA GGCAAAGCCC AGTTCCACTA CGTCACGAGA TAAATCCAAC 
AAGACAACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327476.1
CDS	1649..7120
Translation	MDDSSILRRRGLQKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQATLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYTPPSHVLRPTTVMASPHTIRIGPAAPPTLGLFPSTVAPPSSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKYEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_021471801.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471801.1

ORF Insert Sequence:

ATGGATGACT CCAGTATATT AAGGCGAAGA GGCCTTCAGA AGGAGCTGAG TCTTCCTAGA 
AGAGGCAGTT TCTGTCGGAC GAGCAACCGA AAAAGTTTGA TAGTGACATC CAGCACGTCT 
CCAACGCTGC CTCGACCCCA TTCACCACTG CACGGGCATC CAGGAAGCAG CCCATTGGAC 
AGCCCTCGCA ATTTCTCCCC CAACACTGCA GCCCACTTCT CTTTTGTACC AGCTCGAAGC 
CATGGACACA GGGCTGACAG GACTGATGGT CGTCGCTGGT CTCTCGCCTC TCTGCCGTCC 
TCTGGCTATG GAACAAACAC CCCCAGTTCG ACAGTCTCTT CATCATGTTC ATCTCAGGAG 
AAGCTGCACC AGTTGCCCTT CCAGCCCACT GCAGACGAGC TGCATTTCCT CACCAAACAC 
TTCAGCTCGG AGAGCATCAC GGATGAGGAT GGGAGACGAT CCCCAGCCAT GCGTCCGCGC 
TCCCGCAGTC TCAGCCCTGG ACGGTCACCA ATATCATTTG ACCATGAGAT TGTCATGATG 
AACCATGTCT ACAAGGAGCG TTTTCCTAAG GCCACTGCTC AGATGGAGGT GCGTCTGGCC 
GATTTCATAT CCTCCTCAGC TCCAGAAAAA GTCATGCCTT TAGCTGATGG CGTCCTGAGC 
TTCATCCACC ACCAGGTTAT CGAACTGTCT CGTGACTGTC TGGACAAATC TCGTGAGGGT 
CTCATCACCT CCCGCTACTT TTATGAGCTA CAAGAGAACC TCGAAAAGCT ACACCAGGAT 
GCTCATGAGC GTTCAGAAAG TGGGGAGGTG ACATTTGTCA CCCAGCTAGT TAAAAAGCTG 
ATGATAATCA TCGCTCGGCC TGCCCGGCTG CTAGAATGTT TGGAGTTTGA CCCGGAGGAG 
TTCTACCACC TGTTAGAGGC AGCTGAAGGT CATGCTAAAG AAGGTCAAGG CATCAAGTCT 
GACATCCCTC GATACATCAT CAGCCAGCTG GGCCTCACCA GGGACCCCCT TGAGGAAATG 
GCCCAGCTGA GCAGCTATGA CAGCGGTAAC CCTGAAACAC CAGAGACAGA GACAGATTCC 
ACAGATAGTC GAGGAGCAAC CGTACAGCCA CTCCAACCTC AGCCTCAACC GCAGCCCAAG 
ACTAAAACAC CTCGCGAGGA AGACTTCGAG ACCATTAAAC TCATCAGTAA TGGAGCCTAT 
GGGGCTGTGT TTCTGGTGAG GCATAAAGAG ACCCGTCAGC GATTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTGATCCT AAGAAACCAG ATCCAGCAGG CCTTTGTGGA AAGAGACATC 
TTGACATTTG CAGAAAACCC TTTTGTTGTC AGCATGTTCT GCTCCTTTGA GACAAGGAGA 
CATCTTTGCA TGGTTATGGA GTATGTGGAG GGTGGGGATT GTGCCACTCT CCTGAAGCAC 
ATTGGAGCTC TGCCTGTGGA CATGGCACGC ATGTACTTTG CTGAAACCGT TCTTGCATTG 
GAATACCTTC ACAACTATGG TATTGTGCAC AGAGACCTCA AACCTGACAA TCTCCTGATT 
ACCTCAATGG GACACGTCAA ACTGACTGAC TTTGGCCTGT CCAAGATTGG TTTAATGAGC 
TTAACAACTA ATCTGTATGA GGGCCACATT GAAAAAGATA CTCGAGAATT CTTGGACAAA 
CAGGTGTGTG GTACTCCTGA ATACATTGCT CCTGAGGTTA TTCTGCGACA GGGTTATGGA 
AAACCAGTTG ACTGGTGGGC CATGGGAGTC ATCTTGTATG AGTTTCTGGT GGGCTGTGCT 
CCTTTCTTTG GAGATACACC TGAAGAACTG TTTGGACAAG TCATCAGTGA TGAGATAATC 
TGGCCAGAAG GTGATGAAGC TTTGCCTCCG GATGCTCAAG ATCTCATCTC CAAACTTCTT 
CGGCAAAACC CTCTAGAGCG TCTTGGGACA GGGAGTGCGT TTGAGGTAAA ACAGCACCGC 
TTCTTTACTG ATCTGGACTG GAACAGCCTC CTGAGGCAGA AGGCCGAGTT CATTCCTCAG 
CTTGAGTCGG AGGATGACAC TAGCTACTTT GACACACGCT CAGATCGTTA TCATCATGTG 
GACTCAGAAG AAGAAGATGA CACAAATGAT GACGACCATT TGGAGATCCG TCAGTTCTCC 
TCCTGTTCGC CACGATTCAG CAAGGTGTAT AGTAGTATGG AGCGTCTTTC CTTGCATGAG 
GAGAAACGCA CCCCTCCTCC TACTAAGCGC AGTCTGAGTG AAGAGGGAGG AGAGCGGATC 
GACAGTCTAA GTGGCCTAAA GTCCAGAGAC CGAAGCTGGT TGGTGGGCTC ACCTGAAATT 
CTCAGAAAGC GTCTCTCTGT TTCCGAGTCT TCTCACACCG AGAGTGACTC GAGTCCACCT 
CTGACTGTTC GTCGCCGCTG CTGCTCGGCC ATCATTGAAA TGCCTCGCTT CGCCATATCC 
AGTGAAGAGG ATAGCTGTGT GGGGAGCAGG AAGACCCCTG TGAGAGGCCC TCGAGGGGAT 
GATCTGCCTC AGGCCATACC AGAGCTCCCT GTAGAGAGAG AGCTCAGGCT GGATGAATCG 
CCCACCACGC CAGGATCCAC TTCCAGTCAA ATCAGCCAGG CCACACTCAC ACCTGGTAGT 
TCTGGTGATG TGTTAGATCG TGCTTCTCGC TATAGTGCTG AAGTTTCTGA AAACTCGACT 
CCAAGAGCCA TCAGTGATTT GGCGGCTCGA AGAGCTCGCC ACAGACTGCT TTCAGGAGAT 
ACAGATAAAC ACACATCACG TCCACTTAGC AAAGTCATCA AATCAGCTTC AGCAACAACC 
CTGTCACTCA TGATCCCTGC TGATCACCAT GGTGCTTCTC CACTGGCCAG TCCCATGTCG 
CCTCACTCTC TCTCTTCGAA CCCCTCATCA CGTGACTCCT CGCCCAGCCG TGACCTTTCC 
CCAGCTGTCT GTAGTGTTAA ACCTGCCATA ATCATCCACC GAGCTGGAAA GAAATATGGT 
TTTACTTTAC GTGCAATCCG GGTTTACATG GGAGACACAG ACATATATAC TGTCCATCAC 
ATGGTCTGGC ATGTGGAGGA TGGAGGACCT GCTCATGAGG CTGGACTGAG AGAGGGAGAT 
CTCATCACAC ATGTTAACGG AGAGCCTGTC CATGGACTTG TGCACACAGA AGTTGTTGAG 
CTCATTCTTA AGAGTGGCAG TAAGGTGTCC ATCTCTGCTA CACCCTTTGA AAATACATCA 
ATTAAGGTTG GTCCTGCTCG CAAAACTAGC TACAAGTCCA AAATGGCCCG TCGCAACAAA 
AGAACCAAAA CTAGAGAAGG ACAAGACAGT AAGAAGAGGA ATTCCCTCTT CCGTAAGATC 
ACCAAACAGG CGACCCTTCT TCACACCAGT CGCAGCCTGT CCTCTCTGAA TCGCTCGGTC 
TCTTCTGGTG AGAGCGTCCC AGGCTCTCCT ACTCACATGT CGCCTCGTTC GCCCACACAG 
GGCTGCCGTT CAACACCTGA CTCCGCCCAC TCAGTTGGTG GAAACTCATC CCAAAGTAGC 
TCGCCTAGCT CCAGTGTGCC AAACTCTCCT GCCAGTTCTG GCCAGATCAG ACCCAGCTCC 
TTGCATGGTC TGGCCCCCAA GCTGCAGCGC CAGTACCGCT CCCCCCGCCG GAAGTCTGCA 
GGCAACATCC CTTTATCCCC TCTGGCTCGT ACACCATCGC CCACTCCACA GAGCACTTCT 
CCCCAGCGTT CCCCTTCTCC CCTGCCAAGC CATGGGCTCA TCACTTCTTC AATAGGCCAG 
TCTTTTCCTG TCAAACTGCA CTCTTCACCT CCATTAGTAA GACAGATATC TCGGCCAAAG 
AGTGCAGACC CTCCTCGCTC TCCTTTGCTG AAACGAGTCC AGTCCGCAGA GAAACTCGCT 
GCCTCTCTTT CTTCTTCATC CTCCTCTCCT TCTCCCTCTT CAGCTGCTGA TAAAAAGCTC 
CCAGTTGGTG CCAGCCGCAA ACACAGTCTT GATACCTCAC ATTCAGAGTT TAAAAAAGAG 
ATGCTGCAGC GAGACCCTAG TCTACAGAGC CTGCAAGAGT CAGCCAGTGA GACTCTTATG 
GGAGGAAGAG TGTCTCCTGC AGAAAAGGGA AGCCTACAGA AAAGTTCAGT GCGTAAACTG 
GGCAGGCAAG AAGGTCCGGA GTCTGGAACG GGAACTCTGG GACTTGTTCC TGGAAAGAGT 
AAATTGAAAG ACAAGCTGTC TGCCATACGT GCTGAACGCC GCGAATCTCT ACAAAAGCAG 
GATGCCATCC ATGAAGTGGA TTCATCTGAA GATGAGACAG ACGAAGGGTC GGAAGACAGC 
CAAGATGGGC GCAGAACTTG CTATACTCCT CCTAGCCATG TCTTGAGGCC TACAACTGTA 
ATGGCCTCCC CCCATACCAT CAGAATTGGG CCAGCGGCTC CACCAACACT GGGACTTTTT 
CCTTCAACAG TTGCACCGCC AAGCTCACCT CAGCTCATCA GAGGACTGCA GATACAAAAC 
CAAAGTACCA CTCAATCTCA GACACAAACT TCAACCTCAC AACCAGCGCA AGCTGCAGTT 
AAACCCCCAG AACAGAAACC AATAACCTCG GTTAAAGACT CAAGTTCAAC GATAATTGAT 
GATTCGAAAA AACAGTCACA GGCACAAGAC ATTCAGTCCA GACCTTCTCC ACATCAACCC 
AGTCCTCTGT CTAGTACCTT TTCCACCCAA GGCAGCGCCT TTACCTCTGT TAGTAAAGAC 
CCATTTGTCA AGCCCACCAC ACCTCCATTG GATCCCCGGA GGACCCCAAA AATGTCTGAG 
CCAAGGTCAA AAACCAGTGG TAGAACTGAT TCTAGCACTA CTTCTGGAGT AGCCAGGGAT 
ACCCCAGTTG CTACTACCCC AACAGGAGGA ATCACTAAGG AGATGAAGGA GGCTGACAAT 
CGCAGACAGG CTGCAGCTGC TGCTGCTGCC TCTGCTGCCG CTGCTACCAC CTCTGCATCC 
ACTGCCTCTG AAAGTGGAAT GGATAAAGTT GTGTCTCAGC TCGCCACTGT GGCCAAGAGT 
GTGCTTGGAC CTGTCAAGCT CAATATCCCA GGTACTAAAG AGACCATGGA ACGAACAAAG 
GACCAGCGTA CCCAGGCAGA TGCCACCCAC CCAAAGATAA AAGAATGTCG CCTGGAGCAC 
CCTGATTCTC CTGACCAATT GAGTCCATCT GCTGCTTCGG GCTCCCAGTC TCCACGACTG 
ACTGAATCTC CATCTAAACA AACATCATCC AAATATGAGC CAGCCTCAAC TTCTCAGAGA 
CCTTTAGAGG TCCCAGGGAC TTTCAAAAGA CAAACTTCTC CAATCCCTCA AGCTAGGGAA 
GAGCATCAGG ATAGGTCAGG AACTCTGCAA AGTGGCACCA CATCAAGGCC TAGGTCTGAA 
GCACAATCAC AGACCCAGCA GGCAAAGCCC AGTTCCACTA CGTCACGAGA TAAATCCAAC 
AAGACAACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_021471802.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327477.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X3, mRNA.

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CloneID	ODa144730
Clone ID Related Accession (Same CDS sequence)	XM_021471802.1
Accession Version	XM_021471802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394654.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACAG GTGGGCCAGA CGGGGATCGC GGTATAAATC GCATCTTCAG CAGGTGCTCA 
GAATTGCATT CCAGACTTCT CCATTTAACA CCTTCATCGC TCGCGCTGAG GATGTCCTGC 
GTCTCATGGC AGAGGCGGCT GGAGGAAGAG GAGGTTCTTC AACAGATTTT AGAGCGCTGG 
GAGCAGAGCG CACTCAGAAA CCATGTGGAT CTAACAGCTC ATGAGCGTTC AGAAAGTGGG 
GAGGTGACAT TTGTCACCCA GCTAGTTAAA AAGCTGATGA TAATCATCGC TCGGCCTGCC 
CGGCTGCTAG AATGTTTGGA GTTTGACCCG GAGGAGTTCT ACCACCTGTT AGAGGCAGCT 
GAAGGTCATG CTAAAGAAGG TCAAGGCATC AAGTCTGACA TCCCTCGATA CATCATCAGC 
CAGCTGGGCC TCACCAGGGA CCCCCTTGAG GAAATGGCCC AGCTGAGCAG CTATGACAGC 
GGTAACCCTG AAACACCAGA GACAGAGACA GATTCCACAG ATAGTCGAGG AGCAACCGTA 
CAGCCACTCC AACCTCAGCC TCAACCGCAG CCCAAGACTA AAACACCTCG CGAGGAAGAC 
TTCGAGACCA TTAAACTCAT CAGTAATGGA GCCTATGGGG CTGTGTTTCT GGTGAGGCAT 
AAAGAGACCC GTCAGCGATT TGCCATGAAG AAGATCAACA AGCAGAACCT GATCCTAAGA 
AACCAGATCC AGCAGGCCTT TGTGGAAAGA GACATCTTGA CATTTGCAGA AAACCCTTTT 
GTTGTCAGCA TGTTCTGCTC CTTTGAGACA AGGAGACATC TTTGCATGGT TATGGAGTAT 
GTGGAGGGTG GGGATTGTGC CACTCTCCTG AAGCACATTG GAGCTCTGCC TGTGGACATG 
GCACGCATGT ACTTTGCTGA AACCGTTCTT GCATTGGAAT ACCTTCACAA CTATGGTATT 
GTGCACAGAG ACCTCAAACC TGACAATCTC CTGATTACCT CAATGGGACA CGTCAAACTG 
ACTGACTTTG GCCTGTCCAA GATTGGTTTA ATGAGCTTAA CAACTAATCT GTATGAGGGC 
CACATTGAAA AAGATACTCG AGAATTCTTG GACAAACAGG TGTGTGGTAC TCCTGAATAC 
ATTGCTCCTG AGGTTATTCT GCGACAGGGT TATGGAAAAC CAGTTGACTG GTGGGCCATG 
GGAGTCATCT TGTATGAGTT TCTGGTGGGC TGTGCTCCTT TCTTTGGAGA TACACCTGAA 
GAACTGTTTG GACAAGTCAT CAGTGATGAG ATAATCTGGC CAGAAGGTGA TGAAGCTTTG 
CCTCCGGATG CTCAAGATCT CATCTCCAAA CTTCTTCGGC AAAACCCTCT AGAGCGTCTT 
GGGACAGGGA GTGCGTTTGA GGTAAAACAG CACCGCTTCT TTACTGATCT GGACTGGAAC 
AGCCTCCTGA GGCAGAAGGC CGAGTTCATT CCTCAGCTTG AGTCGGAGGA TGACACTAGC 
TACTTTGACA CACGCTCAGA TCGTTATCAT CATGTGGACT CAGAAGAAGA AGATGACACA 
AATGATGACG ACCATTTGGA GATCCGTCAG TTCTCCTCCT GTTCGCCACG ATTCAGCAAG 
GTGTATAGTA GTATGGAGCG TCTTTCCTTG CATGAGGAGA AACGCACCCC TCCTCCTACT 
AAGCGCAGTC TGAGTGAAGA GGGAGGAGAG CGGATCGACA GTCTAAGTGG CCTAAAGTCC 
AGAGACCGAA GCTGGTTGGT GGGCTCACCT GAAATTCTCA GAAAGCGTCT CTCTGTTTCC 
GAGTCTTCTC ACACCGAGAG TGACTCGAGT CCACCTCTGA CTGTTCGTCG CCGCTGCTGC 
TCGGCCATCA TTGAAATGCC TCGCTTCGCC ATATCCAGTG AAGAGGATAG CTGTGTGGGG 
AGCAGGAAGA CCCCTGTGAG AGGCCCTCGA GGGGATGATC TGCCTCAGGC CATACCAGAG 
CTCCCTGTAG AGAGAGAGCT CAGGCTGGAT GAATCGCCCA CCACGCCAGG ATCCACTTCC 
AGTCAAATCA GCCAGGCCAC ACTCACACCT GGTAGTTCTG GTGATGTGTT AGATCGTGCT 
TCTCGCTATA GTGCTGAAGT TTCTGAAAAC TCGACTCCAA GAGCCATCAG TGATTTGGCG 
GCTCGAAGAG CTCGCCACAG ACTGCTTTCA GGAGATACAG ATAAACACAC ATCACGTCCA 
CTTAGCAAAG TCATCAAATC AGCTTCAGCA ACAACCCTGT CACTCATGAT CCCTGCTGAT 
CACCATGGTG CTTCTCCACT GGCCAGTCCC ATGTCGCCTC ACTCTCTCTC TTCGAACCCC 
TCATCACGTG ACTCCTCGCC CAGCCGTGAC CTTTCCCCAG CTGTCTGTAG TGTTAAACCT 
GCCATAATCA TCCACCGAGC TGGAAAGAAA TATGGTTTTA CTTTACGTGC AATCCGGGTT 
TACATGGGAG ACACAGACAT ATATACTGTC CATCACATGG TCTGGCATGT GGAGGATGGA 
GGACCTGCTC ATGAGGCTGG ACTGAGAGAG GGAGATCTCA TCACACATGT TAACGGAGAG 
CCTGTCCATG GACTTGTGCA CACAGAAGTT GTTGAGCTCA TTCTTAAGAG TGGCAGTAAG 
GTGTCCATCT CTGCTACACC CTTTGAAAAT ACATCAATTA AGGTTGGTCC TGCTCGCAAA 
ACTAGCTACA AGTCCAAAAT GGCCCGTCGC AACAAAAGAA CCAAAACTAG AGAAGGACAA 
GACAGTAAGA AGAGGAATTC CCTCTTCCGT AAGATCACCA AACAGGCGAC CCTTCTTCAC 
ACCAGTCGCA GCCTGTCCTC TCTGAATCGC TCGGTCTCTT CTGGTGAGAG CGTCCCAGGC 
TCTCCTACTC ACATGTCGCC TCGTTCGCCC ACACAGGGCT GCCGTTCAAC ACCTGACTCC 
GCCCACTCAG TTGGTGGAAA CTCATCCCAA AGTAGCTCGC CTAGCTCCAG TGTGCCAAAC 
TCTCCTGCCA GTTCTGGCCA GATCAGACCC AGCTCCTTGC ATGGTCTGGC CCCCAAGCTG 
CAGCGCCAGT ACCGCTCCCC CCGCCGGAAG TCTGCAGGCA ACATCCCTTT ATCCCCTCTG 
GCTCGTACAC CATCGCCCAC TCCACAGAGC ACTTCTCCCC AGCGTTCCCC TTCTCCCCTG 
CCAAGCCATG GGCTCATCAC TTCTTCAATA GGCCAGTCTT TTCCTGTCAA ACTGCACTCT 
TCACCTCCAT TAGTAAGACA GATATCTCGG CCAAAGAGTG CAGACCCTCC TCGCTCTCCT 
TTGCTGAAAC GAGTCCAGTC CGCAGAGAAA CTCGCTGCCT CTCTTTCTTC TTCATCCTCC 
TCTCCTTCTC CCTCTTCAGC TGCTGATAAA AAGCTCCCAG TTGGTGCCAG CCGCAAACAC 
AGTCTTGATA CCTCACATTC AGAGTTTAAA AAAGAGATGC TGCAGCGAGA CCCTAGTCTA 
CAGAGCCTGC AAGAGTCAGC CAGTGAGACT CTTATGGGAG GAAGAGTGTC TCCTGCAGAA 
AAGGGAAGCC TACAGAAAAG TTCAGTGCGT AAACTGGGCA GGCAAGAAGG TCCGGAGTCT 
GGAACGGGAA CTCTGGGACT TGTTCCTGGA AAGAGTAAAT TGAAAGACAA GCTGTCTGCC 
ATACGTGCTG AACGCCGCGA ATCTCTACAA AAGCAGGATG CCATCCATGA AGTGGATTCA 
TCTGAAGATG AGACAGACGA AGGGTCGGAA GACAGCCAAG ATGGGCGCAG AACTTGCTAT 
ACTCCTCCTA GCCATGTCTT GAGGCCTACA ACTGTAATGG CCTCCCCCCA TACCATCAGA 
ATTGGGCCAG CGGCTCCACC AACACTGGGA CTTTTTCCTT CAACAGTTGC ACCGCCAAGC 
TCACCTCAGC TCATCAGAGG ACTGCAGATA CAAAACCAAA GTACCACTCA ATCTCAGACA 
CAAACTTCAA CCTCACAACC AGCGCAAGCT GCAGTTAAAC CCCCAGAACA GAAACCAATA 
ACCTCGGTTA AAGACTCAAG TTCAACGATA ATTGATGATT CGAAAAAACA GTCACAGGCA 
CAAGACATTC AGTCCAGACC TTCTCCACAT CAACCCAGTC CTCTGTCTAG TACCTTTTCC 
ACCCAAGGCA GCGCCTTTAC CTCTGTTAGT AAAGACCCAT TTGTCAAGCC CACCACACCT 
CCATTGGATC CCCGGAGGAC CCCAAAAATG TCTGAGCCAA GGTCAAAAAC CAGTGGTAGA 
ACTGATTCTA GCACTACTTC TGGAGTAGCC AGGGATACCC CAGTTGCTAC TACCCCAACA 
GGAGGAATCA CTAAGGAGAT GAAGGAGGCT GACAATCGCA GACAGGCTGC AGCTGCTGCT 
GCTGCCTCTG CTGCCGCTGC TACCACCTCT GCATCCACTG CCTCTGAAAG TGGAATGGAT 
AAAGTTGTGT CTCAGCTCGC CACTGTGGCC AAGAGTGTGC TTGGACCTGT CAAGCTCAAT 
ATCCCAGGTA CTAAAGAGAC CATGGAACGA ACAAAGGACC AGCGTACCCA GGCAGATGCC 
ACCCACCCAA AGATAAAAGA ATGTCGCCTG GAGCACCCTG ATTCTCCTGA CCAATTGAGT 
CCATCTGCTG CTTCGGGCTC CCAGTCTCCA CGACTGACTG AATCTCCATC TAAACAAACA 
TCATCCAAAT ATGAGCCAGC CTCAACTTCT CAGAGACCTT TAGAGGTCCC AGGGACTTTC 
AAAAGACAAA CTTCTCCAAT CCCTCAAGCT AGGGAAGAGC ATCAGGATAG GTCAGGAACT 
CTGCAAAGTG GCACCACATC AAGGCCTAGG TCTGAAGCAC AATCACAGAC CCAGCAGGCA 
AAGCCCAGTT CCACTACGTC ACGAGATAAA TCCAACAAGA CAACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327477.1
CDS	132..5039
Translation	MVTGGPDGDRGINRIFSRCSELHSRLLHLTPSSLALRMSCVSWQRRLEEEEVLQQILERWEQSALRNHVDLTAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQATLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYTPPSHVLRPTTVMASPHTIRIGPAAPPTLGLFPSTVAPPSSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKYEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_021471802.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471802.1

ORF Insert Sequence:

ATGGTGACAG GTGGGCCAGA CGGGGATCGC GGTATAAATC GCATCTTCAG CAGGTGCTCA 
GAATTGCATT CCAGACTTCT CCATTTAACA CCTTCATCGC TCGCGCTGAG GATGTCCTGC 
GTCTCATGGC AGAGGCGGCT GGAGGAAGAG GAGGTTCTTC AACAGATTTT AGAGCGCTGG 
GAGCAGAGCG CACTCAGAAA CCATGTGGAT CTAACAGCTC ATGAGCGTTC AGAAAGTGGG 
GAGGTGACAT TTGTCACCCA GCTAGTTAAA AAGCTGATGA TAATCATCGC TCGGCCTGCC 
CGGCTGCTAG AATGTTTGGA GTTTGACCCG GAGGAGTTCT ACCACCTGTT AGAGGCAGCT 
GAAGGTCATG CTAAAGAAGG TCAAGGCATC AAGTCTGACA TCCCTCGATA CATCATCAGC 
CAGCTGGGCC TCACCAGGGA CCCCCTTGAG GAAATGGCCC AGCTGAGCAG CTATGACAGC 
GGTAACCCTG AAACACCAGA GACAGAGACA GATTCCACAG ATAGTCGAGG AGCAACCGTA 
CAGCCACTCC AACCTCAGCC TCAACCGCAG CCCAAGACTA AAACACCTCG CGAGGAAGAC 
TTCGAGACCA TTAAACTCAT CAGTAATGGA GCCTATGGGG CTGTGTTTCT GGTGAGGCAT 
AAAGAGACCC GTCAGCGATT TGCCATGAAG AAGATCAACA AGCAGAACCT GATCCTAAGA 
AACCAGATCC AGCAGGCCTT TGTGGAAAGA GACATCTTGA CATTTGCAGA AAACCCTTTT 
GTTGTCAGCA TGTTCTGCTC CTTTGAGACA AGGAGACATC TTTGCATGGT TATGGAGTAT 
GTGGAGGGTG GGGATTGTGC CACTCTCCTG AAGCACATTG GAGCTCTGCC TGTGGACATG 
GCACGCATGT ACTTTGCTGA AACCGTTCTT GCATTGGAAT ACCTTCACAA CTATGGTATT 
GTGCACAGAG ACCTCAAACC TGACAATCTC CTGATTACCT CAATGGGACA CGTCAAACTG 
ACTGACTTTG GCCTGTCCAA GATTGGTTTA ATGAGCTTAA CAACTAATCT GTATGAGGGC 
CACATTGAAA AAGATACTCG AGAATTCTTG GACAAACAGG TGTGTGGTAC TCCTGAATAC 
ATTGCTCCTG AGGTTATTCT GCGACAGGGT TATGGAAAAC CAGTTGACTG GTGGGCCATG 
GGAGTCATCT TGTATGAGTT TCTGGTGGGC TGTGCTCCTT TCTTTGGAGA TACACCTGAA 
GAACTGTTTG GACAAGTCAT CAGTGATGAG ATAATCTGGC CAGAAGGTGA TGAAGCTTTG 
CCTCCGGATG CTCAAGATCT CATCTCCAAA CTTCTTCGGC AAAACCCTCT AGAGCGTCTT 
GGGACAGGGA GTGCGTTTGA GGTAAAACAG CACCGCTTCT TTACTGATCT GGACTGGAAC 
AGCCTCCTGA GGCAGAAGGC CGAGTTCATT CCTCAGCTTG AGTCGGAGGA TGACACTAGC 
TACTTTGACA CACGCTCAGA TCGTTATCAT CATGTGGACT CAGAAGAAGA AGATGACACA 
AATGATGACG ACCATTTGGA GATCCGTCAG TTCTCCTCCT GTTCGCCACG ATTCAGCAAG 
GTGTATAGTA GTATGGAGCG TCTTTCCTTG CATGAGGAGA AACGCACCCC TCCTCCTACT 
AAGCGCAGTC TGAGTGAAGA GGGAGGAGAG CGGATCGACA GTCTAAGTGG CCTAAAGTCC 
AGAGACCGAA GCTGGTTGGT GGGCTCACCT GAAATTCTCA GAAAGCGTCT CTCTGTTTCC 
GAGTCTTCTC ACACCGAGAG TGACTCGAGT CCACCTCTGA CTGTTCGTCG CCGCTGCTGC 
TCGGCCATCA TTGAAATGCC TCGCTTCGCC ATATCCAGTG AAGAGGATAG CTGTGTGGGG 
AGCAGGAAGA CCCCTGTGAG AGGCCCTCGA GGGGATGATC TGCCTCAGGC CATACCAGAG 
CTCCCTGTAG AGAGAGAGCT CAGGCTGGAT GAATCGCCCA CCACGCCAGG ATCCACTTCC 
AGTCAAATCA GCCAGGCCAC ACTCACACCT GGTAGTTCTG GTGATGTGTT AGATCGTGCT 
TCTCGCTATA GTGCTGAAGT TTCTGAAAAC TCGACTCCAA GAGCCATCAG TGATTTGGCG 
GCTCGAAGAG CTCGCCACAG ACTGCTTTCA GGAGATACAG ATAAACACAC ATCACGTCCA 
CTTAGCAAAG TCATCAAATC AGCTTCAGCA ACAACCCTGT CACTCATGAT CCCTGCTGAT 
CACCATGGTG CTTCTCCACT GGCCAGTCCC ATGTCGCCTC ACTCTCTCTC TTCGAACCCC 
TCATCACGTG ACTCCTCGCC CAGCCGTGAC CTTTCCCCAG CTGTCTGTAG TGTTAAACCT 
GCCATAATCA TCCACCGAGC TGGAAAGAAA TATGGTTTTA CTTTACGTGC AATCCGGGTT 
TACATGGGAG ACACAGACAT ATATACTGTC CATCACATGG TCTGGCATGT GGAGGATGGA 
GGACCTGCTC ATGAGGCTGG ACTGAGAGAG GGAGATCTCA TCACACATGT TAACGGAGAG 
CCTGTCCATG GACTTGTGCA CACAGAAGTT GTTGAGCTCA TTCTTAAGAG TGGCAGTAAG 
GTGTCCATCT CTGCTACACC CTTTGAAAAT ACATCAATTA AGGTTGGTCC TGCTCGCAAA 
ACTAGCTACA AGTCCAAAAT GGCCCGTCGC AACAAAAGAA CCAAAACTAG AGAAGGACAA 
GACAGTAAGA AGAGGAATTC CCTCTTCCGT AAGATCACCA AACAGGCGAC CCTTCTTCAC 
ACCAGTCGCA GCCTGTCCTC TCTGAATCGC TCGGTCTCTT CTGGTGAGAG CGTCCCAGGC 
TCTCCTACTC ACATGTCGCC TCGTTCGCCC ACACAGGGCT GCCGTTCAAC ACCTGACTCC 
GCCCACTCAG TTGGTGGAAA CTCATCCCAA AGTAGCTCGC CTAGCTCCAG TGTGCCAAAC 
TCTCCTGCCA GTTCTGGCCA GATCAGACCC AGCTCCTTGC ATGGTCTGGC CCCCAAGCTG 
CAGCGCCAGT ACCGCTCCCC CCGCCGGAAG TCTGCAGGCA ACATCCCTTT ATCCCCTCTG 
GCTCGTACAC CATCGCCCAC TCCACAGAGC ACTTCTCCCC AGCGTTCCCC TTCTCCCCTG 
CCAAGCCATG GGCTCATCAC TTCTTCAATA GGCCAGTCTT TTCCTGTCAA ACTGCACTCT 
TCACCTCCAT TAGTAAGACA GATATCTCGG CCAAAGAGTG CAGACCCTCC TCGCTCTCCT 
TTGCTGAAAC GAGTCCAGTC CGCAGAGAAA CTCGCTGCCT CTCTTTCTTC TTCATCCTCC 
TCTCCTTCTC CCTCTTCAGC TGCTGATAAA AAGCTCCCAG TTGGTGCCAG CCGCAAACAC 
AGTCTTGATA CCTCACATTC AGAGTTTAAA AAAGAGATGC TGCAGCGAGA CCCTAGTCTA 
CAGAGCCTGC AAGAGTCAGC CAGTGAGACT CTTATGGGAG GAAGAGTGTC TCCTGCAGAA 
AAGGGAAGCC TACAGAAAAG TTCAGTGCGT AAACTGGGCA GGCAAGAAGG TCCGGAGTCT 
GGAACGGGAA CTCTGGGACT TGTTCCTGGA AAGAGTAAAT TGAAAGACAA GCTGTCTGCC 
ATACGTGCTG AACGCCGCGA ATCTCTACAA AAGCAGGATG CCATCCATGA AGTGGATTCA 
TCTGAAGATG AGACAGACGA AGGGTCGGAA GACAGCCAAG ATGGGCGCAG AACTTGCTAT 
ACTCCTCCTA GCCATGTCTT GAGGCCTACA ACTGTAATGG CCTCCCCCCA TACCATCAGA 
ATTGGGCCAG CGGCTCCACC AACACTGGGA CTTTTTCCTT CAACAGTTGC ACCGCCAAGC 
TCACCTCAGC TCATCAGAGG ACTGCAGATA CAAAACCAAA GTACCACTCA ATCTCAGACA 
CAAACTTCAA CCTCACAACC AGCGCAAGCT GCAGTTAAAC CCCCAGAACA GAAACCAATA 
ACCTCGGTTA AAGACTCAAG TTCAACGATA ATTGATGATT CGAAAAAACA GTCACAGGCA 
CAAGACATTC AGTCCAGACC TTCTCCACAT CAACCCAGTC CTCTGTCTAG TACCTTTTCC 
ACCCAAGGCA GCGCCTTTAC CTCTGTTAGT AAAGACCCAT TTGTCAAGCC CACCACACCT 
CCATTGGATC CCCGGAGGAC CCCAAAAATG TCTGAGCCAA GGTCAAAAAC CAGTGGTAGA 
ACTGATTCTA GCACTACTTC TGGAGTAGCC AGGGATACCC CAGTTGCTAC TACCCCAACA 
GGAGGAATCA CTAAGGAGAT GAAGGAGGCT GACAATCGCA GACAGGCTGC AGCTGCTGCT 
GCTGCCTCTG CTGCCGCTGC TACCACCTCT GCATCCACTG CCTCTGAAAG TGGAATGGAT 
AAAGTTGTGT CTCAGCTCGC CACTGTGGCC AAGAGTGTGC TTGGACCTGT CAAGCTCAAT 
ATCCCAGGTA CTAAAGAGAC CATGGAACGA ACAAAGGACC AGCGTACCCA GGCAGATGCC 
ACCCACCCAA AGATAAAAGA ATGTCGCCTG GAGCACCCTG ATTCTCCTGA CCAATTGAGT 
CCATCTGCTG CTTCGGGCTC CCAGTCTCCA CGACTGACTG AATCTCCATC TAAACAAACA 
TCATCCAAAT ATGAGCCAGC CTCAACTTCT CAGAGACCTT TAGAGGTCCC AGGGACTTTC 
AAAAGACAAA CTTCTCCAAT CCCTCAAGCT AGGGAAGAGC ATCAGGATAG GTCAGGAACT 
CTGCAAAGTG GCACCACATC AAGGCCTAGG TCTGAAGCAC AATCACAGAC CCAGCAGGCA 
AAGCCCAGTT CCACTACGTC ACGAGATAAA TCCAACAAGA CAACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_021474605.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330280.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X9, mRNA.

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CloneID	ODa146864
Clone ID Related Accession (Same CDS sequence)	XM_021474605.1
Accession Version	XM_021474605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395380.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATGA AGAAAAGAAA GGAAAGACTT GGAGCCCCTG ACCTACACAT ACAAATCTGG 
CCAACTGCTC AGTACCGTTG TCATGGCAAC AGCAGCCTCC TGCAGCATCT CCTGTCCCCT 
TCTTCCTCCC CCTCTGCTCT GTTCTCCCCA TCCCCGGCTG GACCCGAGGG GCCTGCGTCC 
GCCCCTGTCC TTGCTCCCGG CGCCAACCCC TTCTTCTGGA GCTGTCGGAC GAGCAACCGA 
AAGAGTTTGA TAGTGACATC CAGCACGTCT CCAACGCTGC CTCGACCCCA TTCACCACTG 
CACGGGCATC CAGGAAGCAG CCCATTGGAC AGCCCTCGCA ATTTCTCCCC CAACACTGCA 
GCCCACTTCT CTTTTGTACC AGCTCGAAGC CATGGACACA GGGCTGACAG GACTGATGGT 
CGTCGCTGGT CTCTCGCCTC TCTGCCGTCC TCTGGCTATG GAACAAACAC CCCCAGTTCG 
ACAGTCTCTT CATCATGTTC ATCTCAGGAG AAGCTGCACC AGTTGCCCTT CCAGCCCACT 
GCAGACGAGC TGCATTTCCT CACCAAACAC TTCAGCTCGG AGAGCATCAC GGATGAGGAT 
GGGAGACGAT CCCCAGCCAT GCGTCCGCGC TCCCGCAGTC TCAGCCCTGG ACGGTCACCA 
ATATCATTTG ACCATGAGAT TGTCATGATG AACCATGTCT ACAAGGAGCG TTTTCCTAAG 
GCCACTGCTC AGATGGAGGT GCGTCTGGCC GATTTCATAT CCTCCTCAGC TCCAGAAAAA 
GTCATGCCTT TAGCTGATGG CGTCCTGAGC TTCATCCACC ACCAGGTTAT CGAACTGTCT 
CGTGACTGTC TGGACAAATC TCGTGAGGGT CTCATCACCT CCCGCTACTT CTATGAGCTA 
CAAGAGAACC TCGAAAAGCT ACACCAGGAT GCTCATGAGC GTTCAGAAAG TGGGGAGGTG 
ACATTTGTCA CCCAGCTAGT TAAAAAGCTG ATGATAATCA TCGCTCGGCC TGCCCGGCTG 
CTAGAATGTT TGGAGTTTGA CCCGGAGGAG TTCTACCACC TGTTAGAGGC AGCTGAAGGT 
CATGCTAAAG AAGGTCAAGG CATCAAGTCT GACATCCCTC GATACATCAT CAGCCAGCTG 
GGCCTCACCA GGGACCCCCT TGAGGAAATG GCCCAGCTGA GCAGCTATGA CAGCGGTAAC 
CCTGAAACAC CAGAGACAGA GACAGATTCC ACAGATAGTC GAGGAGCAAC CGTACAGCCA 
CTCCAACCTC AGCCTCAACC GCAGCCCAAG ACTAAAACAC CTCGCGAGGA AGACTTCGAG 
ACCATTAAAC TCATCAGTAA TGGAGCCTAT GGGGCTGTGT TTCTGGTGAG GCATAAAGAG 
ACCCGTCAGC GATTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT AAGAAACCAG 
ATCCAGCAGG CCTTTGTGGA AAGAGACATC TTGACATTTG CAGAAAACCC TTTTGTTGTC 
AGCATGTTCT GCTCCTTTGA GACAAGGAGA CATCTTTGCA TGGTTATGGA GTATGTGGAG 
GGTGGGGATT GTGCCACTCT CCTGAAGCAC ATTGGAGCTC TGCCTGTGGA TATGGCACGC 
ATGTACTTTG CTGAAACCGT TCTTGCATTG GAATACCTTC ACAACTATGG TATTGTGCAC 
AGAGACCTCA AACCTGACAA TCTCCTGATT ACCTCAATGG GACACGTCAA ACTGACTGAC 
TTTGGCCTGT CCAAGATTGG TTTAATGAGC TTAACAACTA ATCTGTATGA GGGCCACATT 
GAAAAAGATA CTCGAGAATT CTTGGACAAA CAGGTGTGTG GTACTCCTGA ATACATTGCT 
CCTGAGGTTA TTCTGCGACA GGGTTATGGA AAACCAGTTG ACTGGTGGGC CATGGGAGTC 
ATCTTGTATG AGTTTCTGGT GGGCTGTGCT CCTTTCTTTG GAGATACACC TGAAGAACTG 
TTTGGACAAG TCATCAGTGA TGAGATAATC TGGCCAGAAG GTGATGAAGC TTTGCCTCCG 
GATGCTCAAG ATCTCATCTC CAAACTTCTT CGGCAAAACC CTCTAGAGCG TCTTGGGACA 
GGGAGTGCGT TTGAGGTAAA ACAGCACCGC TTCTTTACTG ATCTGGACTG GAACAGCCTC 
CTGAGGCAGA AGGCCGAGTT CATTCCTCAG CTGGAGTCGG AGGATGACAC TAGCTACTTT 
GACACACGCT CAGATCGTTA TCATCATGTG GACTCAGAAG AAGAAGATGA CACAAATGAT 
GACGACCATT TGGAGATCCG TCAGTTCTCC TCCTGTTCGC CACGATTCAG CAAGGTGTAT 
AGTAGTATGG AGCGTCTTTC CTTGCATGAG GAGAAACGCA CCCCTCCTCC TACTAAGCGC 
AGTCTGAGTG AAGAGGGAGG AGAGCGGATC GACAGTCTAA GTGGCCTAAA GTCCAGAGAC 
CGAAGCTGGT TGGTGGGCTC ACCTGAAATT CTCAGAAAGC GTCTCTCTGT TTCCGAGTCT 
TCTCACACCG AGAGTGACTC GAGTCCACCT CTGACTGTTC GTCGCCGCTG CTGCTCGGCC 
ATCATTGAAA TGCCTCGCTT CGCCATATCC AGTGAAGAGG ATAGCTGTGT GGGGAGCAGG 
AAGACCCCTG TGAGAGGCCC TCGAGGGGAT GATCTGCCTC AGGCCATACC AGAGCTCCCT 
GTAGAGAGAG AGCTCAGGCT GGATGAATCG CCCACCACGC CAGGATCCAC TTCCAGTCAA 
ATCAGCCAGT CCACACTCAC ACCTGGTAGT TCTGGTGATG TGTTAGATCG TGCTTCTCGC 
TATAGTGCTG AAGTTTCTGA AAACTCGACT CCAAGAGCCA TCAGTGATTT GGCGGCTCGA 
AGAGCTCGCC ACAGACTGCT TTCAGGAGAT ACAGATAAAC ACACATCACG TCCACTTAGC 
AAAGTCATCA AATCAGCTTC AGCAACAACC CTGTCACTCA TGATCCCTGC TGATCACCAT 
GGTGCTTCTC CACTGGCCAG TCCCATGTCG CCTCACTCTC TCTCTTCGAA CCCCTCATCA 
CGTGACTCCT CGCCCAGCCG TGACCTTTCC CCAGCTGTCT GTAGTGTTAA ACCTGCCATA 
ATCATCCACC GAGCTGGAAA GAAATATGGT TTTACTTTAC GTGCAATCCG GGTTTACATG 
GGAGACACAG ACATATATAC TGTCCATCAC ATGGTCTGGC ATGTGGAGGA TGGAGGACCT 
GCTCATGAGG CTGGACTGAG AGAGGGAGAT CTCATCACAC ATGTTAACGG AGAGCCTGTC 
CATGGACTTG TGCACACAGA AGTTGTTGAG CTCATTCTTA AGAGTGGCAG TAAGGTGTCC 
ATCTCTGCTA CACCCTTTGA AAATACATCA ATTAAGGTTG GTCCTGCTCG CAAAACTAGC 
TACAAGTCCA AAATGGCCCG TCGCAACAAA AGAACCAAAA CTAGAGAAGG ACAAGATAGT 
AAGAAGAGGA ATTCCCTCTT TCGTAAGATC ACCAAACAGG CGACCCTTCT TCACACCAGT 
CGCAGCCTGT CCTCTCTGAA TCGCTCGGTC TCTTCTGGTG AGAGCGTCCC AGGCTCTCCT 
ACTCACATGT CACCTCATTC GCCCACACAG GGCTGTCGTT CAACACCTGA CTCCGCCCAC 
TCAGTTGGTG GAAACTCATC CCAAAGTAGC TCGCCTAGCT CCAGTGTGCC AAACTCTCCT 
GCCAGTTCTG GCCAGATCAG ACCCAGCTCC TTGCATGGTC TGGCCCCCAA GCTGCAGAGA 
CAGTACCGCT CCCCCCGCCG GAAGTCTGCA GGCAACATCC CTTTATCCCC TCTGGCTCGT 
ACACCATCGC CCACTCCACA GAGCACTTCT CCCCAGCGTT CCCCTTCTCC CCTGCCAAGC 
CACGGGCTCA TCACTTCTTC AATAGGGCAG TCTTTTCCTG TCAAACTGCA CTCTTCACCT 
CCATTAGTAA GACAGATATC TCGGCCAAAG AGTGCAGACC CTCCTCGCTC TCCTTTGCTA 
AAACGAGTCC AGTCCGCTGA GAAACTCGCT GCCTCTCTTT CTTCTTCATC CTCCTCTCCT 
TCTCCCTCTT CGGCTGCTGA TAAAAAGCTC CCAGTTGGTG CCAGCCGCAA ACACAGTCTT 
GATACCTCAC ATTCAGAGTT TAAAAAAGAG ATGCTGCAGC GAGACCCTAG TCTACAGAGC 
CTGCAAGAGT CAGCCAGTGA GACTCTTATG GGAGGAAGAG TGTCTCCTGC AGAAAAGGGA 
AGCCTGCAGA AAAGTTCAGT GCGTAAACTG GGCAGGCAAG AAGGTCCGGA GTCTGGAACA 
GGAACTCTGG GACTTGTTCC TGGAAAGAGT AAATTGAAAG AAAAGCTGTC TGCCATACGT 
GCTGAACGCC GCGAATCTCT TCAAAAGCAG GATGCCATCC ATGAAGTGGA TTCATCTGAA 
GATGAGACAG ACGAAGGGTC GGAAGACAGC CAAGATGGGC GCAGAACTTG CTATACTCCT 
CCTAGCCATG TCTTGAGGCC TACAACTGTA ATGGCCTCTC CCCATACCAT CAGAATGGGG 
CCAGCGGCTC CACCAACACT GGGACTTTTT CCTTCAACAG TTGCACCGCC AAGCTCACCT 
CAGCTCATCA GAGGACTGCA GATACAAAAC CAAAGTACCA CTCAATCTCA GACACAAACT 
TCAACCTCAC AACAAGCGCA AGCTGCAGTT AAACCCCCAG AACAGAAACC AATAACCTCT 
GTTAAAGACT CAAGTTCAAC AATAATTGAT GACTCGAAAA AACAGTCACA GGCACAAGAA 
ATTCAGTCCA GACCTTCTCC ACATCAACCC AGTCCTCTGT CTAGTACCTT TTCCACCCAA 
GGCAGCGCCT TTACCTCTGT TAGTAAAGAC CCATTTGTCA AGCCCACCAC ACCTCCATTG 
GATCCCCGGA GGACCCTAAA AATGTCTGAG CCAAGGTCAA AAACCAGTGG TAGAACTGAT 
TCTAGCACTA CTTCTGGAGT AGCCAGGGAT ACCCCAGTTG CGACTACCCC AACAGGAGGA 
ATCACTAAGG AGATGAAGGA GGCTGACAAT CGCAGACAGG CTGCAGCTGC TGCTGCTGCC 
TCTGCCGCTG CTGCTACCAC CTCTGCATCC ACTGCCTCTG AAAGTGGAAT GGATAAAGTT 
GTGTCTCAGC TCACCACTGT GGCCAAGAGT GTGCTTGGAC CTGTCAAGCT CAATATCCCA 
GGTACTAAAG AGACCATGGA ACGAACAAAG GACCAGCGTA CCCAGGCAGA TGCCACCCAC 
CCAAAGATAA AAGAATGTCG CCTGGAGCAC CCTGATTCTC CTGACCAATT GAGTCCATCT 
GCTGCTTCGG GCTCCCAGTC TCCACGACTG ACTGAATCTC CATCTAAACA AACATCATCC 
AAATCTGAGC CAGCCTCAAC TTCTCAGAGA CCTTTAGAGG TCCCAGGGAC TTTCAAAAGA 
CAAACTTCTC CAATCCCTCA AGCTGGGGAA GAGCATCAGG ATAGGTCGGG AACTCTGCAA 
AGTGGCACCA CATCAAGGCC TAGGTCTGAA GCACAATCAC AGACCCAGCA GGCAAAGCCC 
AGTTCCACTA CGTCACGAGA TAAATCCAAC AAGACAACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330280.1
CDS	199..5820
Translation	MNMKKRKERLGAPDLHIQIWPTAQYRCHGNSSLLQHLLSPSSSPSALFSPSPAGPEGPASAPVLAPGANPFFWSCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPHSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKEKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYTPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPSSPQLIRGLQIQNQSTTQSQTQTSTSQQAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQEIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTLKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLTTVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAGEEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_021474605.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474605.1

ORF Insert Sequence:

ATGAACATGA AGAAAAGAAA GGAAAGACTT GGAGCCCCTG ACCTACACAT ACAAATCTGG 
CCAACTGCTC AGTACCGTTG TCATGGCAAC AGCAGCCTCC TGCAGCATCT CCTGTCCCCT 
TCTTCCTCCC CCTCTGCTCT GTTCTCCCCA TCCCCGGCTG GACCCGAGGG GCCTGCGTCC 
GCCCCTGTCC TTGCTCCCGG CGCCAACCCC TTCTTCTGGA GCTGTCGGAC GAGCAACCGA 
AAGAGTTTGA TAGTGACATC CAGCACGTCT CCAACGCTGC CTCGACCCCA TTCACCACTG 
CACGGGCATC CAGGAAGCAG CCCATTGGAC AGCCCTCGCA ATTTCTCCCC CAACACTGCA 
GCCCACTTCT CTTTTGTACC AGCTCGAAGC CATGGACACA GGGCTGACAG GACTGATGGT 
CGTCGCTGGT CTCTCGCCTC TCTGCCGTCC TCTGGCTATG GAACAAACAC CCCCAGTTCG 
ACAGTCTCTT CATCATGTTC ATCTCAGGAG AAGCTGCACC AGTTGCCCTT CCAGCCCACT 
GCAGACGAGC TGCATTTCCT CACCAAACAC TTCAGCTCGG AGAGCATCAC GGATGAGGAT 
GGGAGACGAT CCCCAGCCAT GCGTCCGCGC TCCCGCAGTC TCAGCCCTGG ACGGTCACCA 
ATATCATTTG ACCATGAGAT TGTCATGATG AACCATGTCT ACAAGGAGCG TTTTCCTAAG 
GCCACTGCTC AGATGGAGGT GCGTCTGGCC GATTTCATAT CCTCCTCAGC TCCAGAAAAA 
GTCATGCCTT TAGCTGATGG CGTCCTGAGC TTCATCCACC ACCAGGTTAT CGAACTGTCT 
CGTGACTGTC TGGACAAATC TCGTGAGGGT CTCATCACCT CCCGCTACTT CTATGAGCTA 
CAAGAGAACC TCGAAAAGCT ACACCAGGAT GCTCATGAGC GTTCAGAAAG TGGGGAGGTG 
ACATTTGTCA CCCAGCTAGT TAAAAAGCTG ATGATAATCA TCGCTCGGCC TGCCCGGCTG 
CTAGAATGTT TGGAGTTTGA CCCGGAGGAG TTCTACCACC TGTTAGAGGC AGCTGAAGGT 
CATGCTAAAG AAGGTCAAGG CATCAAGTCT GACATCCCTC GATACATCAT CAGCCAGCTG 
GGCCTCACCA GGGACCCCCT TGAGGAAATG GCCCAGCTGA GCAGCTATGA CAGCGGTAAC 
CCTGAAACAC CAGAGACAGA GACAGATTCC ACAGATAGTC GAGGAGCAAC CGTACAGCCA 
CTCCAACCTC AGCCTCAACC GCAGCCCAAG ACTAAAACAC CTCGCGAGGA AGACTTCGAG 
ACCATTAAAC TCATCAGTAA TGGAGCCTAT GGGGCTGTGT TTCTGGTGAG GCATAAAGAG 
ACCCGTCAGC GATTTGCCAT GAAGAAGATC AACAAGCAGA ACCTGATCCT AAGAAACCAG 
ATCCAGCAGG CCTTTGTGGA AAGAGACATC TTGACATTTG CAGAAAACCC TTTTGTTGTC 
AGCATGTTCT GCTCCTTTGA GACAAGGAGA CATCTTTGCA TGGTTATGGA GTATGTGGAG 
GGTGGGGATT GTGCCACTCT CCTGAAGCAC ATTGGAGCTC TGCCTGTGGA TATGGCACGC 
ATGTACTTTG CTGAAACCGT TCTTGCATTG GAATACCTTC ACAACTATGG TATTGTGCAC 
AGAGACCTCA AACCTGACAA TCTCCTGATT ACCTCAATGG GACACGTCAA ACTGACTGAC 
TTTGGCCTGT CCAAGATTGG TTTAATGAGC TTAACAACTA ATCTGTATGA GGGCCACATT 
GAAAAAGATA CTCGAGAATT CTTGGACAAA CAGGTGTGTG GTACTCCTGA ATACATTGCT 
CCTGAGGTTA TTCTGCGACA GGGTTATGGA AAACCAGTTG ACTGGTGGGC CATGGGAGTC 
ATCTTGTATG AGTTTCTGGT GGGCTGTGCT CCTTTCTTTG GAGATACACC TGAAGAACTG 
TTTGGACAAG TCATCAGTGA TGAGATAATC TGGCCAGAAG GTGATGAAGC TTTGCCTCCG 
GATGCTCAAG ATCTCATCTC CAAACTTCTT CGGCAAAACC CTCTAGAGCG TCTTGGGACA 
GGGAGTGCGT TTGAGGTAAA ACAGCACCGC TTCTTTACTG ATCTGGACTG GAACAGCCTC 
CTGAGGCAGA AGGCCGAGTT CATTCCTCAG CTGGAGTCGG AGGATGACAC TAGCTACTTT 
GACACACGCT CAGATCGTTA TCATCATGTG GACTCAGAAG AAGAAGATGA CACAAATGAT 
GACGACCATT TGGAGATCCG TCAGTTCTCC TCCTGTTCGC CACGATTCAG CAAGGTGTAT 
AGTAGTATGG AGCGTCTTTC CTTGCATGAG GAGAAACGCA CCCCTCCTCC TACTAAGCGC 
AGTCTGAGTG AAGAGGGAGG AGAGCGGATC GACAGTCTAA GTGGCCTAAA GTCCAGAGAC 
CGAAGCTGGT TGGTGGGCTC ACCTGAAATT CTCAGAAAGC GTCTCTCTGT TTCCGAGTCT 
TCTCACACCG AGAGTGACTC GAGTCCACCT CTGACTGTTC GTCGCCGCTG CTGCTCGGCC 
ATCATTGAAA TGCCTCGCTT CGCCATATCC AGTGAAGAGG ATAGCTGTGT GGGGAGCAGG 
AAGACCCCTG TGAGAGGCCC TCGAGGGGAT GATCTGCCTC AGGCCATACC AGAGCTCCCT 
GTAGAGAGAG AGCTCAGGCT GGATGAATCG CCCACCACGC CAGGATCCAC TTCCAGTCAA 
ATCAGCCAGT CCACACTCAC ACCTGGTAGT TCTGGTGATG TGTTAGATCG TGCTTCTCGC 
TATAGTGCTG AAGTTTCTGA AAACTCGACT CCAAGAGCCA TCAGTGATTT GGCGGCTCGA 
AGAGCTCGCC ACAGACTGCT TTCAGGAGAT ACAGATAAAC ACACATCACG TCCACTTAGC 
AAAGTCATCA AATCAGCTTC AGCAACAACC CTGTCACTCA TGATCCCTGC TGATCACCAT 
GGTGCTTCTC CACTGGCCAG TCCCATGTCG CCTCACTCTC TCTCTTCGAA CCCCTCATCA 
CGTGACTCCT CGCCCAGCCG TGACCTTTCC CCAGCTGTCT GTAGTGTTAA ACCTGCCATA 
ATCATCCACC GAGCTGGAAA GAAATATGGT TTTACTTTAC GTGCAATCCG GGTTTACATG 
GGAGACACAG ACATATATAC TGTCCATCAC ATGGTCTGGC ATGTGGAGGA TGGAGGACCT 
GCTCATGAGG CTGGACTGAG AGAGGGAGAT CTCATCACAC ATGTTAACGG AGAGCCTGTC 
CATGGACTTG TGCACACAGA AGTTGTTGAG CTCATTCTTA AGAGTGGCAG TAAGGTGTCC 
ATCTCTGCTA CACCCTTTGA AAATACATCA ATTAAGGTTG GTCCTGCTCG CAAAACTAGC 
TACAAGTCCA AAATGGCCCG TCGCAACAAA AGAACCAAAA CTAGAGAAGG ACAAGATAGT 
AAGAAGAGGA ATTCCCTCTT TCGTAAGATC ACCAAACAGG CGACCCTTCT TCACACCAGT 
CGCAGCCTGT CCTCTCTGAA TCGCTCGGTC TCTTCTGGTG AGAGCGTCCC AGGCTCTCCT 
ACTCACATGT CACCTCATTC GCCCACACAG GGCTGTCGTT CAACACCTGA CTCCGCCCAC 
TCAGTTGGTG GAAACTCATC CCAAAGTAGC TCGCCTAGCT CCAGTGTGCC AAACTCTCCT 
GCCAGTTCTG GCCAGATCAG ACCCAGCTCC TTGCATGGTC TGGCCCCCAA GCTGCAGAGA 
CAGTACCGCT CCCCCCGCCG GAAGTCTGCA GGCAACATCC CTTTATCCCC TCTGGCTCGT 
ACACCATCGC CCACTCCACA GAGCACTTCT CCCCAGCGTT CCCCTTCTCC CCTGCCAAGC 
CACGGGCTCA TCACTTCTTC AATAGGGCAG TCTTTTCCTG TCAAACTGCA CTCTTCACCT 
CCATTAGTAA GACAGATATC TCGGCCAAAG AGTGCAGACC CTCCTCGCTC TCCTTTGCTA 
AAACGAGTCC AGTCCGCTGA GAAACTCGCT GCCTCTCTTT CTTCTTCATC CTCCTCTCCT 
TCTCCCTCTT CGGCTGCTGA TAAAAAGCTC CCAGTTGGTG CCAGCCGCAA ACACAGTCTT 
GATACCTCAC ATTCAGAGTT TAAAAAAGAG ATGCTGCAGC GAGACCCTAG TCTACAGAGC 
CTGCAAGAGT CAGCCAGTGA GACTCTTATG GGAGGAAGAG TGTCTCCTGC AGAAAAGGGA 
AGCCTGCAGA AAAGTTCAGT GCGTAAACTG GGCAGGCAAG AAGGTCCGGA GTCTGGAACA 
GGAACTCTGG GACTTGTTCC TGGAAAGAGT AAATTGAAAG AAAAGCTGTC TGCCATACGT 
GCTGAACGCC GCGAATCTCT TCAAAAGCAG GATGCCATCC ATGAAGTGGA TTCATCTGAA 
GATGAGACAG ACGAAGGGTC GGAAGACAGC CAAGATGGGC GCAGAACTTG CTATACTCCT 
CCTAGCCATG TCTTGAGGCC TACAACTGTA ATGGCCTCTC CCCATACCAT CAGAATGGGG 
CCAGCGGCTC CACCAACACT GGGACTTTTT CCTTCAACAG TTGCACCGCC AAGCTCACCT 
CAGCTCATCA GAGGACTGCA GATACAAAAC CAAAGTACCA CTCAATCTCA GACACAAACT 
TCAACCTCAC AACAAGCGCA AGCTGCAGTT AAACCCCCAG AACAGAAACC AATAACCTCT 
GTTAAAGACT CAAGTTCAAC AATAATTGAT GACTCGAAAA AACAGTCACA GGCACAAGAA 
ATTCAGTCCA GACCTTCTCC ACATCAACCC AGTCCTCTGT CTAGTACCTT TTCCACCCAA 
GGCAGCGCCT TTACCTCTGT TAGTAAAGAC CCATTTGTCA AGCCCACCAC ACCTCCATTG 
GATCCCCGGA GGACCCTAAA AATGTCTGAG CCAAGGTCAA AAACCAGTGG TAGAACTGAT 
TCTAGCACTA CTTCTGGAGT AGCCAGGGAT ACCCCAGTTG CGACTACCCC AACAGGAGGA 
ATCACTAAGG AGATGAAGGA GGCTGACAAT CGCAGACAGG CTGCAGCTGC TGCTGCTGCC 
TCTGCCGCTG CTGCTACCAC CTCTGCATCC ACTGCCTCTG AAAGTGGAAT GGATAAAGTT 
GTGTCTCAGC TCACCACTGT GGCCAAGAGT GTGCTTGGAC CTGTCAAGCT CAATATCCCA 
GGTACTAAAG AGACCATGGA ACGAACAAAG GACCAGCGTA CCCAGGCAGA TGCCACCCAC 
CCAAAGATAA AAGAATGTCG CCTGGAGCAC CCTGATTCTC CTGACCAATT GAGTCCATCT 
GCTGCTTCGG GCTCCCAGTC TCCACGACTG ACTGAATCTC CATCTAAACA AACATCATCC 
AAATCTGAGC CAGCCTCAAC TTCTCAGAGA CCTTTAGAGG TCCCAGGGAC TTTCAAAAGA 
CAAACTTCTC CAATCCCTCA AGCTGGGGAA GAGCATCAGG ATAGGTCGGG AACTCTGCAA 
AGTGGCACCA CATCAAGGCC TAGGTCTGAA GCACAATCAC AGACCCAGCA GGCAAAGCCC 
AGTTCCACTA CGTCACGAGA TAAATCCAAC AAGACAACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_021474606.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330281.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X10, mRNA.

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CloneID	ODa146865
Clone ID Related Accession (Same CDS sequence)	XM_021474606.1
Accession Version	XM_021474606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395380.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATGA AGAAAAGAAA GGAAAGACTT GGAGCCCCTG ACCTACACAT ACAAATCTGG 
CCAACTGCTC AGTACCGTTG TCATGGCAAC AGCAGCCTCC TGCAGCATCT CCTGTCCCCT 
TCTTCCTCCC CCTCTGCTCT GTTCTCCCCA TCCCCGGCTG GACCCGAGGG GCCTGCGTCC 
GCCCCTGTCC TTGCTCCCGG CGCCAACCCC TTCTTCTGGA GCTGTCGGAC GAGCAACCGA 
AAGAGTTTGA TAGTGACATC CAGCACGTCT CCAACGCTGC CTCGACCCCA TTCACCACTG 
CACGGGCATC CAGGAAGCAG CCCATTGGAC AGCCCTCGCA ATTTCTCCCC CAACACTGCA 
GCCCACTTCT CTTTTGTACC AGCTCGAAGG ACTGATGGTC GTCGCTGGTC TCTCGCCTCT 
CTGCCGTCCT CTGGCTATGG AACAAACACC CCCAGTTCGA CAGTCTCTTC ATCATGTTCA 
TCTCAGGAGA AGCTGCACCA GTTGCCCTTC CAGCCCACTG CAGACGAGCT GCATTTCCTC 
ACCAAACACT TCAGCTCGGA GAGCATCACG GATGAGGATG GGAGACGATC CCCAGCCATG 
CGTCCGCGCT CCCGCAGTCT CAGCCCTGGA CGGTCACCAA TATCATTTGA CCATGAGATT 
GTCATGATGA ACCATGTCTA CAAGGAGCGT TTTCCTAAGG CCACTGCTCA GATGGAGGTG 
CGTCTGGCCG ATTTCATATC CTCCTCAGCT CCAGAAAAAG TCATGCCTTT AGCTGATGGC 
GTCCTGAGCT TCATCCACCA CCAGGTTATC GAACTGTCTC GTGACTGTCT GGACAAATCT 
CGTGAGGGTC TCATCACCTC CCGCTACTTC TATGAGCTAC AAGAGAACCT CGAAAAGCTA 
CACCAGGATG CTCATGAGCG TTCAGAAAGT GGGGAGGTGA CATTTGTCAC CCAGCTAGTT 
AAAAAGCTGA TGATAATCAT CGCTCGGCCT GCCCGGCTGC TAGAATGTTT GGAGTTTGAC 
CCGGAGGAGT TCTACCACCT GTTAGAGGCA GCTGAAGGTC ATGCTAAAGA AGGTCAAGGC 
ATCAAGTCTG ACATCCCTCG ATACATCATC AGCCAGCTGG GCCTCACCAG GGACCCCCTT 
GAGGAAATGG CCCAGCTGAG CAGCTATGAC AGCGGTAACC CTGAAACACC AGAGACAGAG 
ACAGATTCCA CAGATAGTCG AGGAGCAACC GTACAGCCAC TCCAACCTCA GCCTCAACCG 
CAGCCCAAGA CTAAAACACC TCGCGAGGAA GACTTCGAGA CCATTAAACT CATCAGTAAT 
GGAGCCTATG GGGCTGTGTT TCTGGTGAGG CATAAAGAGA CCCGTCAGCG ATTTGCCATG 
AAGAAGATCA ACAAGCAGAA CCTGATCCTA AGAAACCAGA TCCAGCAGGC CTTTGTGGAA 
AGAGACATCT TGACATTTGC AGAAAACCCT TTTGTTGTCA GCATGTTCTG CTCCTTTGAG 
ACAAGGAGAC ATCTTTGCAT GGTTATGGAG TATGTGGAGG GTGGGGATTG TGCCACTCTC 
CTGAAGCACA TTGGAGCTCT GCCTGTGGAT ATGGCACGCA TGTACTTTGC TGAAACCGTT 
CTTGCATTGG AATACCTTCA CAACTATGGT ATTGTGCACA GAGACCTCAA ACCTGACAAT 
CTCCTGATTA CCTCAATGGG ACACGTCAAA CTGACTGACT TTGGCCTGTC CAAGATTGGT 
TTAATGAGCT TAACAACTAA TCTGTATGAG GGCCACATTG AAAAAGATAC TCGAGAATTC 
TTGGACAAAC AGGTGTGTGG TACTCCTGAA TACATTGCTC CTGAGGTTAT TCTGCGACAG 
GGTTATGGAA AACCAGTTGA CTGGTGGGCC ATGGGAGTCA TCTTGTATGA GTTTCTGGTG 
GGCTGTGCTC CTTTCTTTGG AGATACACCT GAAGAACTGT TTGGACAAGT CATCAGTGAT 
GAGATAATCT GGCCAGAAGG TGATGAAGCT TTGCCTCCGG ATGCTCAAGA TCTCATCTCC 
AAACTTCTTC GGCAAAACCC TCTAGAGCGT CTTGGGACAG GGAGTGCGTT TGAGGTAAAA 
CAGCACCGCT TCTTTACTGA TCTGGACTGG AACAGCCTCC TGAGGCAGAA GGCCGAGTTC 
ATTCCTCAGC TGGAGTCGGA GGATGACACT AGCTACTTTG ACACACGCTC AGATCGTTAT 
CATCATGTGG ACTCAGAAGA AGAAGATGAC ACAAATGATG ACGACCATTT GGAGATCCGT 
CAGTTCTCCT CCTGTTCGCC ACGATTCAGC AAGGTGTATA GTAGTATGGA GCGTCTTTCC 
TTGCATGAGG AGAAACGCAC CCCTCCTCCT ACTAAGCGCA GTCTGAGTGA AGAGGGAGGA 
GAGCGGATCG ACAGTCTAAG TGGCCTAAAG TCCAGAGACC GAAGCTGGTT GGTGGGCTCA 
CCTGAAATTC TCAGAAAGCG TCTCTCTGTT TCCGAGTCTT CTCACACCGA GAGTGACTCG 
AGTCCACCTC TGACTGTTCG TCGCCGCTGC TGCTCGGCCA TCATTGAAAT GCCTCGCTTC 
GCCATATCCA GTGAAGAGGA TAGCTGTGTG GGGAGCAGGA AGACCCCTGT GAGAGGCCCT 
CGAGGGGATG ATCTGCCTCA GGCCATACCA GAGCTCCCTG TAGAGAGAGA GCTCAGGCTG 
GATGAATCGC CCACCACGCC AGGATCCACT TCCAGTCAAA TCAGCCAGTC CACACTCACA 
CCTGGTAGTT CTGGTGATGT GTTAGATCGT GCTTCTCGCT ATAGTGCTGA AGTTTCTGAA 
AACTCGACTC CAAGAGCCAT CAGTGATTTG GCGGCTCGAA GAGCTCGCCA CAGACTGCTT 
TCAGGAGATA CAGATAAACA CACATCACGT CCACTTAGCA AAGTCATCAA ATCAGCTTCA 
GCAACAACCC TGTCACTCAT GATCCCTGCT GATCACCATG GTGCTTCTCC ACTGGCCAGT 
CCCATGTCGC CTCACTCTCT CTCTTCGAAC CCCTCATCAC GTGACTCCTC GCCCAGCCGT 
GACCTTTCCC CAGCTGTCTG TAGTGTTAAA CCTGCCATAA TCATCCACCG AGCTGGAAAG 
AAATATGGTT TTACTTTACG TGCAATCCGG GTTTACATGG GAGACACAGA CATATATACT 
GTCCATCACA TGGTCTGGCA TGTGGAGGAT GGAGGACCTG CTCATGAGGC TGGACTGAGA 
GAGGGAGATC TCATCACACA TGTTAACGGA GAGCCTGTCC ATGGACTTGT GCACACAGAA 
GTTGTTGAGC TCATTCTTAA GAGTGGCAGT AAGGTGTCCA TCTCTGCTAC ACCCTTTGAA 
AATACATCAA TTAAGGTTGG TCCTGCTCGC AAAACTAGCT ACAAGTCCAA AATGGCCCGT 
CGCAACAAAA GAACCAAAAC TAGAGAAGGA CAAGATAGTA AGAAGAGGAA TTCCCTCTTT 
CGTAAGATCA CCAAACAGGC GACCCTTCTT CACACCAGTC GCAGCCTGTC CTCTCTGAAT 
CGCTCGGTCT CTTCTGGTGA GAGCGTCCCA GGCTCTCCTA CTCACATGTC ACCTCATTCG 
CCCACACAGG GCTGTCGTTC AACACCTGAC TCCGCCCACT CAGTTGGTGG AAACTCATCC 
CAAAGTAGCT CGCCTAGCTC CAGTGTGCCA AACTCTCCTG CCAGTTCTGG CCAGATCAGA 
CCCAGCTCCT TGCATGGTCT GGCCCCCAAG CTGCAGAGAC AGTACCGCTC CCCCCGCCGG 
AAGTCTGCAG GCAACATCCC TTTATCCCCT CTGGCTCGTA CACCATCGCC CACTCCACAG 
AGCACTTCTC CCCAGCGTTC CCCTTCTCCC CTGCCAAGCC ACGGGCTCAT CACTTCTTCA 
ATAGGGCAGT CTTTTCCTGT CAAACTGCAC TCTTCACCTC CATTAGTAAG ACAGATATCT 
CGGCCAAAGA GTGCAGACCC TCCTCGCTCT CCTTTGCTAA AACGAGTCCA GTCCGCTGAG 
AAACTCGCTG CCTCTCTTTC TTCTTCATCC TCCTCTCCTT CTCCCTCTTC GGCTGCTGAT 
AAAAAGCTCC CAGTTGGTGC CAGCCGCAAA CACAGTCTTG ATACCTCACA TTCAGAGTTT 
AAAAAAGAGA TGCTGCAGCG AGACCCTAGT CTACAGAGCC TGCAAGAGTC AGCCAGTGAG 
ACTCTTATGG GAGGAAGAGT GTCTCCTGCA GAAAAGGGAA GCCTGCAGAA AAGTTCAGTG 
CGTAAACTGG GCAGGCAAGA AGGTCCGGAG TCTGGAACAG GAACTCTGGG ACTTGTTCCT 
GGAAAGAGTA AATTGAAAGA AAAGCTGTCT GCCATACGTG CTGAACGCCG CGAATCTCTT 
CAAAAGCAGG ATGCCATCCA TGAAGTGGAT TCATCTGAAG ATGAGACAGA CGAAGGGTCG 
GAAGACAGCC AAGATGGGCG CAGAACTTGC TATACTCCTC CTAGCCATGT CTTGAGGCCT 
ACAACTGTAA TGGCCTCTCC CCATACCATC AGAATGGGGC CAGCGGCTCC ACCAACACTG 
GGACTTTTTC CTTCAACAGT TGCACCGCCA AGCTCACCTC AGCTCATCAG AGGACTGCAG 
ATACAAAACC AAAGTACCAC TCAATCTCAG ACACAAACTT CAACCTCACA ACAAGCGCAA 
GCTGCAGTTA AACCCCCAGA ACAGAAACCA ATAACCTCTG TTAAAGACTC AAGTTCAACA 
ATAATTGATG ACTCGAAAAA ACAGTCACAG GCACAAGAAA TTCAGTCCAG ACCTTCTCCA 
CATCAACCCA GTCCTCTGTC TAGTACCTTT TCCACCCAAG GCAGCGCCTT TACCTCTGTT 
AGTAAAGACC CATTTGTCAA GCCCACCACA CCTCCATTGG ATCCCCGGAG GACCCTAAAA 
ATGTCTGAGC CAAGGTCAAA AACCAGTGGT AGAACTGATT CTAGCACTAC TTCTGGAGTA 
GCCAGGGATA CCCCAGTTGC GACTACCCCA ACAGGAGGAA TCACTAAGGA GATGAAGGAG 
GCTGACAATC GCAGACAGGC TGCAGCTGCT GCTGCTGCCT CTGCCGCTGC TGCTACCACC 
TCTGCATCCA CTGCCTCTGA AAGTGGAATG GATAAAGTTG TGTCTCAGCT CACCACTGTG 
GCCAAGAGTG TGCTTGGACC TGTCAAGCTC AATATCCCAG GTACTAAAGA GACCATGGAA 
CGAACAAAGG ACCAGCGTAC CCAGGCAGAT GCCACCCACC CAAAGATAAA AGAATGTCGC 
CTGGAGCACC CTGATTCTCC TGACCAATTG AGTCCATCTG CTGCTTCGGG CTCCCAGTCT 
CCACGACTGA CTGAATCTCC ATCTAAACAA ACATCATCCA AATCTGAGCC AGCCTCAACT 
TCTCAGAGAC CTTTAGAGGT CCCAGGGACT TTCAAAAGAC AAACTTCTCC AATCCCTCAA 
GCTGGGGAAG AGCATCAGGA TAGGTCGGGA ACTCTGCAAA GTGGCACCAC ATCAAGGCCT 
AGGTCTGAAG CACAATCACA GACCCAGCAG GCAAAGCCCA GTTCCACTAC GTCACGAGAT 
AAATCCAACA AGACAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330281.1
CDS	199..5799
Translation	MNMKKRKERLGAPDLHIQIWPTAQYRCHGNSSLLQHLLSPSSSPSALFSPSPAGPEGPASAPVLAPGANPFFWSCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPHSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKEKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYTPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPSSPQLIRGLQIQNQSTTQSQTQTSTSQQAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQEIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTLKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLTTVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAGEEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_021474606.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474606.1

ORF Insert Sequence:

ATGAACATGA AGAAAAGAAA GGAAAGACTT GGAGCCCCTG ACCTACACAT ACAAATCTGG 
CCAACTGCTC AGTACCGTTG TCATGGCAAC AGCAGCCTCC TGCAGCATCT CCTGTCCCCT 
TCTTCCTCCC CCTCTGCTCT GTTCTCCCCA TCCCCGGCTG GACCCGAGGG GCCTGCGTCC 
GCCCCTGTCC TTGCTCCCGG CGCCAACCCC TTCTTCTGGA GCTGTCGGAC GAGCAACCGA 
AAGAGTTTGA TAGTGACATC CAGCACGTCT CCAACGCTGC CTCGACCCCA TTCACCACTG 
CACGGGCATC CAGGAAGCAG CCCATTGGAC AGCCCTCGCA ATTTCTCCCC CAACACTGCA 
GCCCACTTCT CTTTTGTACC AGCTCGAAGG ACTGATGGTC GTCGCTGGTC TCTCGCCTCT 
CTGCCGTCCT CTGGCTATGG AACAAACACC CCCAGTTCGA CAGTCTCTTC ATCATGTTCA 
TCTCAGGAGA AGCTGCACCA GTTGCCCTTC CAGCCCACTG CAGACGAGCT GCATTTCCTC 
ACCAAACACT TCAGCTCGGA GAGCATCACG GATGAGGATG GGAGACGATC CCCAGCCATG 
CGTCCGCGCT CCCGCAGTCT CAGCCCTGGA CGGTCACCAA TATCATTTGA CCATGAGATT 
GTCATGATGA ACCATGTCTA CAAGGAGCGT TTTCCTAAGG CCACTGCTCA GATGGAGGTG 
CGTCTGGCCG ATTTCATATC CTCCTCAGCT CCAGAAAAAG TCATGCCTTT AGCTGATGGC 
GTCCTGAGCT TCATCCACCA CCAGGTTATC GAACTGTCTC GTGACTGTCT GGACAAATCT 
CGTGAGGGTC TCATCACCTC CCGCTACTTC TATGAGCTAC AAGAGAACCT CGAAAAGCTA 
CACCAGGATG CTCATGAGCG TTCAGAAAGT GGGGAGGTGA CATTTGTCAC CCAGCTAGTT 
AAAAAGCTGA TGATAATCAT CGCTCGGCCT GCCCGGCTGC TAGAATGTTT GGAGTTTGAC 
CCGGAGGAGT TCTACCACCT GTTAGAGGCA GCTGAAGGTC ATGCTAAAGA AGGTCAAGGC 
ATCAAGTCTG ACATCCCTCG ATACATCATC AGCCAGCTGG GCCTCACCAG GGACCCCCTT 
GAGGAAATGG CCCAGCTGAG CAGCTATGAC AGCGGTAACC CTGAAACACC AGAGACAGAG 
ACAGATTCCA CAGATAGTCG AGGAGCAACC GTACAGCCAC TCCAACCTCA GCCTCAACCG 
CAGCCCAAGA CTAAAACACC TCGCGAGGAA GACTTCGAGA CCATTAAACT CATCAGTAAT 
GGAGCCTATG GGGCTGTGTT TCTGGTGAGG CATAAAGAGA CCCGTCAGCG ATTTGCCATG 
AAGAAGATCA ACAAGCAGAA CCTGATCCTA AGAAACCAGA TCCAGCAGGC CTTTGTGGAA 
AGAGACATCT TGACATTTGC AGAAAACCCT TTTGTTGTCA GCATGTTCTG CTCCTTTGAG 
ACAAGGAGAC ATCTTTGCAT GGTTATGGAG TATGTGGAGG GTGGGGATTG TGCCACTCTC 
CTGAAGCACA TTGGAGCTCT GCCTGTGGAT ATGGCACGCA TGTACTTTGC TGAAACCGTT 
CTTGCATTGG AATACCTTCA CAACTATGGT ATTGTGCACA GAGACCTCAA ACCTGACAAT 
CTCCTGATTA CCTCAATGGG ACACGTCAAA CTGACTGACT TTGGCCTGTC CAAGATTGGT 
TTAATGAGCT TAACAACTAA TCTGTATGAG GGCCACATTG AAAAAGATAC TCGAGAATTC 
TTGGACAAAC AGGTGTGTGG TACTCCTGAA TACATTGCTC CTGAGGTTAT TCTGCGACAG 
GGTTATGGAA AACCAGTTGA CTGGTGGGCC ATGGGAGTCA TCTTGTATGA GTTTCTGGTG 
GGCTGTGCTC CTTTCTTTGG AGATACACCT GAAGAACTGT TTGGACAAGT CATCAGTGAT 
GAGATAATCT GGCCAGAAGG TGATGAAGCT TTGCCTCCGG ATGCTCAAGA TCTCATCTCC 
AAACTTCTTC GGCAAAACCC TCTAGAGCGT CTTGGGACAG GGAGTGCGTT TGAGGTAAAA 
CAGCACCGCT TCTTTACTGA TCTGGACTGG AACAGCCTCC TGAGGCAGAA GGCCGAGTTC 
ATTCCTCAGC TGGAGTCGGA GGATGACACT AGCTACTTTG ACACACGCTC AGATCGTTAT 
CATCATGTGG ACTCAGAAGA AGAAGATGAC ACAAATGATG ACGACCATTT GGAGATCCGT 
CAGTTCTCCT CCTGTTCGCC ACGATTCAGC AAGGTGTATA GTAGTATGGA GCGTCTTTCC 
TTGCATGAGG AGAAACGCAC CCCTCCTCCT ACTAAGCGCA GTCTGAGTGA AGAGGGAGGA 
GAGCGGATCG ACAGTCTAAG TGGCCTAAAG TCCAGAGACC GAAGCTGGTT GGTGGGCTCA 
CCTGAAATTC TCAGAAAGCG TCTCTCTGTT TCCGAGTCTT CTCACACCGA GAGTGACTCG 
AGTCCACCTC TGACTGTTCG TCGCCGCTGC TGCTCGGCCA TCATTGAAAT GCCTCGCTTC 
GCCATATCCA GTGAAGAGGA TAGCTGTGTG GGGAGCAGGA AGACCCCTGT GAGAGGCCCT 
CGAGGGGATG ATCTGCCTCA GGCCATACCA GAGCTCCCTG TAGAGAGAGA GCTCAGGCTG 
GATGAATCGC CCACCACGCC AGGATCCACT TCCAGTCAAA TCAGCCAGTC CACACTCACA 
CCTGGTAGTT CTGGTGATGT GTTAGATCGT GCTTCTCGCT ATAGTGCTGA AGTTTCTGAA 
AACTCGACTC CAAGAGCCAT CAGTGATTTG GCGGCTCGAA GAGCTCGCCA CAGACTGCTT 
TCAGGAGATA CAGATAAACA CACATCACGT CCACTTAGCA AAGTCATCAA ATCAGCTTCA 
GCAACAACCC TGTCACTCAT GATCCCTGCT GATCACCATG GTGCTTCTCC ACTGGCCAGT 
CCCATGTCGC CTCACTCTCT CTCTTCGAAC CCCTCATCAC GTGACTCCTC GCCCAGCCGT 
GACCTTTCCC CAGCTGTCTG TAGTGTTAAA CCTGCCATAA TCATCCACCG AGCTGGAAAG 
AAATATGGTT TTACTTTACG TGCAATCCGG GTTTACATGG GAGACACAGA CATATATACT 
GTCCATCACA TGGTCTGGCA TGTGGAGGAT GGAGGACCTG CTCATGAGGC TGGACTGAGA 
GAGGGAGATC TCATCACACA TGTTAACGGA GAGCCTGTCC ATGGACTTGT GCACACAGAA 
GTTGTTGAGC TCATTCTTAA GAGTGGCAGT AAGGTGTCCA TCTCTGCTAC ACCCTTTGAA 
AATACATCAA TTAAGGTTGG TCCTGCTCGC AAAACTAGCT ACAAGTCCAA AATGGCCCGT 
CGCAACAAAA GAACCAAAAC TAGAGAAGGA CAAGATAGTA AGAAGAGGAA TTCCCTCTTT 
CGTAAGATCA CCAAACAGGC GACCCTTCTT CACACCAGTC GCAGCCTGTC CTCTCTGAAT 
CGCTCGGTCT CTTCTGGTGA GAGCGTCCCA GGCTCTCCTA CTCACATGTC ACCTCATTCG 
CCCACACAGG GCTGTCGTTC AACACCTGAC TCCGCCCACT CAGTTGGTGG AAACTCATCC 
CAAAGTAGCT CGCCTAGCTC CAGTGTGCCA AACTCTCCTG CCAGTTCTGG CCAGATCAGA 
CCCAGCTCCT TGCATGGTCT GGCCCCCAAG CTGCAGAGAC AGTACCGCTC CCCCCGCCGG 
AAGTCTGCAG GCAACATCCC TTTATCCCCT CTGGCTCGTA CACCATCGCC CACTCCACAG 
AGCACTTCTC CCCAGCGTTC CCCTTCTCCC CTGCCAAGCC ACGGGCTCAT CACTTCTTCA 
ATAGGGCAGT CTTTTCCTGT CAAACTGCAC TCTTCACCTC CATTAGTAAG ACAGATATCT 
CGGCCAAAGA GTGCAGACCC TCCTCGCTCT CCTTTGCTAA AACGAGTCCA GTCCGCTGAG 
AAACTCGCTG CCTCTCTTTC TTCTTCATCC TCCTCTCCTT CTCCCTCTTC GGCTGCTGAT 
AAAAAGCTCC CAGTTGGTGC CAGCCGCAAA CACAGTCTTG ATACCTCACA TTCAGAGTTT 
AAAAAAGAGA TGCTGCAGCG AGACCCTAGT CTACAGAGCC TGCAAGAGTC AGCCAGTGAG 
ACTCTTATGG GAGGAAGAGT GTCTCCTGCA GAAAAGGGAA GCCTGCAGAA AAGTTCAGTG 
CGTAAACTGG GCAGGCAAGA AGGTCCGGAG TCTGGAACAG GAACTCTGGG ACTTGTTCCT 
GGAAAGAGTA AATTGAAAGA AAAGCTGTCT GCCATACGTG CTGAACGCCG CGAATCTCTT 
CAAAAGCAGG ATGCCATCCA TGAAGTGGAT TCATCTGAAG ATGAGACAGA CGAAGGGTCG 
GAAGACAGCC AAGATGGGCG CAGAACTTGC TATACTCCTC CTAGCCATGT CTTGAGGCCT 
ACAACTGTAA TGGCCTCTCC CCATACCATC AGAATGGGGC CAGCGGCTCC ACCAACACTG 
GGACTTTTTC CTTCAACAGT TGCACCGCCA AGCTCACCTC AGCTCATCAG AGGACTGCAG 
ATACAAAACC AAAGTACCAC TCAATCTCAG ACACAAACTT CAACCTCACA ACAAGCGCAA 
GCTGCAGTTA AACCCCCAGA ACAGAAACCA ATAACCTCTG TTAAAGACTC AAGTTCAACA 
ATAATTGATG ACTCGAAAAA ACAGTCACAG GCACAAGAAA TTCAGTCCAG ACCTTCTCCA 
CATCAACCCA GTCCTCTGTC TAGTACCTTT TCCACCCAAG GCAGCGCCTT TACCTCTGTT 
AGTAAAGACC CATTTGTCAA GCCCACCACA CCTCCATTGG ATCCCCGGAG GACCCTAAAA 
ATGTCTGAGC CAAGGTCAAA AACCAGTGGT AGAACTGATT CTAGCACTAC TTCTGGAGTA 
GCCAGGGATA CCCCAGTTGC GACTACCCCA ACAGGAGGAA TCACTAAGGA GATGAAGGAG 
GCTGACAATC GCAGACAGGC TGCAGCTGCT GCTGCTGCCT CTGCCGCTGC TGCTACCACC 
TCTGCATCCA CTGCCTCTGA AAGTGGAATG GATAAAGTTG TGTCTCAGCT CACCACTGTG 
GCCAAGAGTG TGCTTGGACC TGTCAAGCTC AATATCCCAG GTACTAAAGA GACCATGGAA 
CGAACAAAGG ACCAGCGTAC CCAGGCAGAT GCCACCCACC CAAAGATAAA AGAATGTCGC 
CTGGAGCACC CTGATTCTCC TGACCAATTG AGTCCATCTG CTGCTTCGGG CTCCCAGTCT 
CCACGACTGA CTGAATCTCC ATCTAAACAA ACATCATCCA AATCTGAGCC AGCCTCAACT 
TCTCAGAGAC CTTTAGAGGT CCCAGGGACT TTCAAAAGAC AAACTTCTCC AATCCCTCAA 
GCTGGGGAAG AGCATCAGGA TAGGTCGGGA ACTCTGCAAA GTGGCACCAC ATCAAGGCCT 
AGGTCTGAAG CACAATCACA GACCCAGCAG GCAAAGCCCA GTTCCACTAC GTCACGAGAT 
AAATCCAACA AGACAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_021474607.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330282.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X11, mRNA.

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CloneID	ODa146866
Clone ID Related Accession (Same CDS sequence)	XM_021474607.1
Accession Version	XM_021474607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395380.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA TCCTCACTGG AAAGCAGCTT CTGCCTCTGT CCAGCAGTAT GCATGGAGGA 
GTGAGTCAGC TGGCCTGGCA GCAGCACACA GGGGAGCCCA ATAACTTGGT CCGCATGAGG 
AGTCAGTCCT TTGGCCAGTC TGCCCCGTCT CTCACTGCGG GACTGAAGGA GCTGAGTCTT 
CCTAGAAGAG GCAGTTTCTG TCGGACGAGC AACCGAAAGA GTTTGATAGT GACATCCAGC 
ACGTCTCCAA CGCTGCCTCG ACCCCATTCA CCACTGCACG GGCATCCAGG AAGCAGCCCA 
TTGGACAGCC CTCGCAATTT CTCCCCCAAC ACTGCAGCCC ACTTCTCTTT TGTACCAGCT 
CGAAGCCATG GACACAGGGC TGACAGGACT GATGGTCGTC GCTGGTCTCT CGCCTCTCTG 
CCGTCCTCTG GCTATGGAAC AAACACCCCC AGTTCGACAG TCTCTTCATC ATGTTCATCT 
CAGGAGAAGC TGCACCAGTT GCCCTTCCAG CCCACTGCAG ACGAGCTGCA TTTCCTCACC 
AAACACTTCA GCTCGGAGAG CATCACGGAT GAGGATGGGA GACGATCCCC AGCCATGCGT 
CCGCGCTCCC GCAGTCTCAG CCCTGGACGG TCACCAATAT CATTTGACCA TGAGATTGTC 
ATGATGAACC ATGTCTACAA GGAGCGTTTT CCTAAGGCCA CTGCTCAGAT GGAGGTGCGT 
CTGGCCGATT TCATATCCTC CTCAGCTCCA GAAAAAGTCA TGCCTTTAGC TGATGGCGTC 
CTGAGCTTCA TCCACCACCA GGTTATCGAA CTGTCTCGTG ACTGTCTGGA CAAATCTCGT 
GAGGGTCTCA TCACCTCCCG CTACTTCTAT GAGCTACAAG AGAACCTCGA AAAGCTACAC 
CAGGATGCTC ATGAGCGTTC AGAAAGTGGG GAGGTGACAT TTGTCACCCA GCTAGTTAAA 
AAGCTGATGA TAATCATCGC TCGGCCTGCC CGGCTGCTAG AATGTTTGGA GTTTGACCCG 
GAGGAGTTCT ACCACCTGTT AGAGGCAGCT GAAGGTCATG CTAAAGAAGG TCAAGGCATC 
AAGTCTGACA TCCCTCGATA CATCATCAGC CAGCTGGGCC TCACCAGGGA CCCCCTTGAG 
GAAATGGCCC AGCTGAGCAG CTATGACAGC GGTAACCCTG AAACACCAGA GACAGAGACA 
GATTCCACAG ATAGTCGAGG AGCAACCGTA CAGCCACTCC AACCTCAGCC TCAACCGCAG 
CCCAAGACTA AAACACCTCG CGAGGAAGAC TTCGAGACCA TTAAACTCAT CAGTAATGGA 
GCCTATGGGG CTGTGTTTCT GGTGAGGCAT AAAGAGACCC GTCAGCGATT TGCCATGAAG 
AAGATCAACA AGCAGAACCT GATCCTAAGA AACCAGATCC AGCAGGCCTT TGTGGAAAGA 
GACATCTTGA CATTTGCAGA AAACCCTTTT GTTGTCAGCA TGTTCTGCTC CTTTGAGACA 
AGGAGACATC TTTGCATGGT TATGGAGTAT GTGGAGGGTG GGGATTGTGC CACTCTCCTG 
AAGCACATTG GAGCTCTGCC TGTGGATATG GCACGCATGT ACTTTGCTGA AACCGTTCTT 
GCATTGGAAT ACCTTCACAA CTATGGTATT GTGCACAGAG ACCTCAAACC TGACAATCTC 
CTGATTACCT CAATGGGACA CGTCAAACTG ACTGACTTTG GCCTGTCCAA GATTGGTTTA 
ATGAGCTTAA CAACTAATCT GTATGAGGGC CACATTGAAA AAGATACTCG AGAATTCTTG 
GACAAACAGG TGTGTGGTAC TCCTGAATAC ATTGCTCCTG AGGTTATTCT GCGACAGGGT 
TATGGAAAAC CAGTTGACTG GTGGGCCATG GGAGTCATCT TGTATGAGTT TCTGGTGGGC 
TGTGCTCCTT TCTTTGGAGA TACACCTGAA GAACTGTTTG GACAAGTCAT CAGTGATGAG 
ATAATCTGGC CAGAAGGTGA TGAAGCTTTG CCTCCGGATG CTCAAGATCT CATCTCCAAA 
CTTCTTCGGC AAAACCCTCT AGAGCGTCTT GGGACAGGGA GTGCGTTTGA GGTAAAACAG 
CACCGCTTCT TTACTGATCT GGACTGGAAC AGCCTCCTGA GGCAGAAGGC CGAGTTCATT 
CCTCAGCTGG AGTCGGAGGA TGACACTAGC TACTTTGACA CACGCTCAGA TCGTTATCAT 
CATGTGGACT CAGAAGAAGA AGATGACACA AATGATGACG ACCATTTGGA GATCCGTCAG 
TTCTCCTCCT GTTCGCCACG ATTCAGCAAG GTGTATAGTA GTATGGAGCG TCTTTCCTTG 
CATGAGGAGA AACGCACCCC TCCTCCTACT AAGCGCAGTC TGAGTGAAGA GGGAGGAGAG 
CGGATCGACA GTCTAAGTGG CCTAAAGTCC AGAGACCGAA GCTGGTTGGT GGGCTCACCT 
GAAATTCTCA GAAAGCGTCT CTCTGTTTCC GAGTCTTCTC ACACCGAGAG TGACTCGAGT 
CCACCTCTGA CTGTTCGTCG CCGCTGCTGC TCGGCCATCA TTGAAATGCC TCGCTTCGCC 
ATATCCAGTG AAGAGGATAG CTGTGTGGGG AGCAGGAAGA CCCCTGTGAG AGGCCCTCGA 
GGGGATGATC TGCCTCAGGC CATACCAGAG CTCCCTGTAG AGAGAGAGCT CAGGCTGGAT 
GAATCGCCCA CCACGCCAGG ATCCACTTCC AGTCAAATCA GCCAGTCCAC ACTCACACCT 
GGTAGTTCTG GTGATGTGTT AGATCGTGCT TCTCGCTATA GTGCTGAAGT TTCTGAAAAC 
TCGACTCCAA GAGCCATCAG TGATTTGGCG GCTCGAAGAG CTCGCCACAG ACTGCTTTCA 
GGAGATACAG ATAAACACAC ATCACGTCCA CTTAGCAAAG TCATCAAATC AGCTTCAGCA 
ACAACCCTGT CACTCATGAT CCCTGCTGAT CACCATGGTG CTTCTCCACT GGCCAGTCCC 
ATGTCGCCTC ACTCTCTCTC TTCGAACCCC TCATCACGTG ACTCCTCGCC CAGCCGTGAC 
CTTTCCCCAG CTGTCTGTAG TGTTAAACCT GCCATAATCA TCCACCGAGC TGGAAAGAAA 
TATGGTTTTA CTTTACGTGC AATCCGGGTT TACATGGGAG ACACAGACAT ATATACTGTC 
CATCACATGG TCTGGCATGT GGAGGATGGA GGACCTGCTC ATGAGGCTGG ACTGAGAGAG 
GGAGATCTCA TCACACATGT TAACGGAGAG CCTGTCCATG GACTTGTGCA CACAGAAGTT 
GTTGAGCTCA TTCTTAAGAG TGGCAGTAAG GTGTCCATCT CTGCTACACC CTTTGAAAAT 
ACATCAATTA AGGTTGGTCC TGCTCGCAAA ACTAGCTACA AGTCCAAAAT GGCCCGTCGC 
AACAAAAGAA CCAAAACTAG AGAAGGACAA GATAGTAAGA AGAGGAATTC CCTCTTTCGT 
AAGATCACCA AACAGGCGAC CCTTCTTCAC ACCAGTCGCA GCCTGTCCTC TCTGAATCGC 
TCGGTCTCTT CTGGTGAGAG CGTCCCAGGC TCTCCTACTC ACATGTCACC TCATTCGCCC 
ACACAGGGCT GTCGTTCAAC ACCTGACTCC GCCCACTCAG TTGGTGGAAA CTCATCCCAA 
AGTAGCTCGC CTAGCTCCAG TGTGCCAAAC TCTCCTGCCA GTTCTGGCCA GATCAGACCC 
AGCTCCTTGC ATGGTCTGGC CCCCAAGCTG CAGAGACAGT ACCGCTCCCC CCGCCGGAAG 
TCTGCAGGCA ACATCCCTTT ATCCCCTCTG GCTCGTACAC CATCGCCCAC TCCACAGAGC 
ACTTCTCCCC AGCGTTCCCC TTCTCCCCTG CCAAGCCACG GGCTCATCAC TTCTTCAATA 
GGGCAGTCTT TTCCTGTCAA ACTGCACTCT TCACCTCCAT TAGTAAGACA GATATCTCGG 
CCAAAGAGTG CAGACCCTCC TCGCTCTCCT TTGCTAAAAC GAGTCCAGTC CGCTGAGAAA 
CTCGCTGCCT CTCTTTCTTC TTCATCCTCC TCTCCTTCTC CCTCTTCGGC TGCTGATAAA 
AAGCTCCCAG TTGGTGCCAG CCGCAAACAC AGTCTTGATA CCTCACATTC AGAGTTTAAA 
AAAGAGATGC TGCAGCGAGA CCCTAGTCTA CAGAGCCTGC AAGAGTCAGC CAGTGAGACT 
CTTATGGGAG GAAGAGTGTC TCCTGCAGAA AAGGGAAGCC TGCAGAAAAG TTCAGTGCGT 
AAACTGGGCA GGCAAGAAGG TCCGGAGTCT GGAACAGGAA CTCTGGGACT TGTTCCTGGA 
AAGAGTAAAT TGAAAGAAAA GCTGTCTGCC ATACGTGCTG AACGCCGCGA ATCTCTTCAA 
AAGCAGGATG CCATCCATGA AGTGGATTCA TCTGAAGATG AGACAGACGA AGGGTCGGAA 
GACAGCCAAG ATGGGCGCAG AACTTGCTAT ACTCCTCCTA GCCATGTCTT GAGGCCTACA 
ACTGTAATGG CCTCTCCCCA TACCATCAGA ATGGGGCCAG CGGCTCCACC AACACTGGGA 
CTTTTTCCTT CAACAGTTGC ACCGCCAAGC TCACCTCAGC TCATCAGAGG ACTGCAGATA 
CAAAACCAAA GTACCACTCA ATCTCAGACA CAAACTTCAA CCTCACAACA AGCGCAAGCT 
GCAGTTAAAC CCCCAGAACA GAAACCAATA ACCTCTGTTA AAGACTCAAG TTCAACAATA 
ATTGATGACT CGAAAAAACA GTCACAGGCA CAAGAAATTC AGTCCAGACC TTCTCCACAT 
CAACCCAGTC CTCTGTCTAG TACCTTTTCC ACCCAAGGCA GCGCCTTTAC CTCTGTTAGT 
AAAGACCCAT TTGTCAAGCC CACCACACCT CCATTGGATC CCCGGAGGAC CCTAAAAATG 
TCTGAGCCAA GGTCAAAAAC CAGTGGTAGA ACTGATTCTA GCACTACTTC TGGAGTAGCC 
AGGGATACCC CAGTTGCGAC TACCCCAACA GGAGGAATCA CTAAGGAGAT GAAGGAGGCT 
GACAATCGCA GACAGGCTGC AGCTGCTGCT GCTGCCTCTG CCGCTGCTGC TACCACCTCT 
GCATCCACTG CCTCTGAAAG TGGAATGGAT AAAGTTGTGT CTCAGCTCAC CACTGTGGCC 
AAGAGTGTGC TTGGACCTGT CAAGCTCAAT ATCCCAGGTA CTAAAGAGAC CATGGAACGA 
ACAAAGGACC AGCGTACCCA GGCAGATGCC ACCCACCCAA AGATAAAAGA ATGTCGCCTG 
GAGCACCCTG ATTCTCCTGA CCAATTGAGT CCATCTGCTG CTTCGGGCTC CCAGTCTCCA 
CGACTGACTG AATCTCCATC TAAACAAACA TCATCCAAAT CTGAGCCAGC CTCAACTTCT 
CAGAGACCTT TAGAGGTCCC AGGGACTTTC AAAAGACAAA CTTCTCCAAT CCCTCAAGCT 
GGGGAAGAGC ATCAGGATAG GTCGGGAACT CTGCAAAGTG GCACCACATC AAGGCCTAGG 
TCTGAAGCAC AATCACAGAC CCAGCAGGCA AAGCCCAGTT CCACTACGTC ACGAGATAAA 
TCCAACAAGA CAACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330282.1
CDS	123..5720
Translation	MAGILTGKQLLPLSSSMHGGVSQLAWQQHTGEPNNLVRMRSQSFGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPHSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKEKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYTPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPSSPQLIRGLQIQNQSTTQSQTQTSTSQQAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQEIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTLKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLTTVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAGEEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_021474607.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474607.1

ORF Insert Sequence:

ATGGCTGGCA TCCTCACTGG AAAGCAGCTT CTGCCTCTGT CCAGCAGTAT GCATGGAGGA 
GTGAGTCAGC TGGCCTGGCA GCAGCACACA GGGGAGCCCA ATAACTTGGT CCGCATGAGG 
AGTCAGTCCT TTGGCCAGTC TGCCCCGTCT CTCACTGCGG GACTGAAGGA GCTGAGTCTT 
CCTAGAAGAG GCAGTTTCTG TCGGACGAGC AACCGAAAGA GTTTGATAGT GACATCCAGC 
ACGTCTCCAA CGCTGCCTCG ACCCCATTCA CCACTGCACG GGCATCCAGG AAGCAGCCCA 
TTGGACAGCC CTCGCAATTT CTCCCCCAAC ACTGCAGCCC ACTTCTCTTT TGTACCAGCT 
CGAAGCCATG GACACAGGGC TGACAGGACT GATGGTCGTC GCTGGTCTCT CGCCTCTCTG 
CCGTCCTCTG GCTATGGAAC AAACACCCCC AGTTCGACAG TCTCTTCATC ATGTTCATCT 
CAGGAGAAGC TGCACCAGTT GCCCTTCCAG CCCACTGCAG ACGAGCTGCA TTTCCTCACC 
AAACACTTCA GCTCGGAGAG CATCACGGAT GAGGATGGGA GACGATCCCC AGCCATGCGT 
CCGCGCTCCC GCAGTCTCAG CCCTGGACGG TCACCAATAT CATTTGACCA TGAGATTGTC 
ATGATGAACC ATGTCTACAA GGAGCGTTTT CCTAAGGCCA CTGCTCAGAT GGAGGTGCGT 
CTGGCCGATT TCATATCCTC CTCAGCTCCA GAAAAAGTCA TGCCTTTAGC TGATGGCGTC 
CTGAGCTTCA TCCACCACCA GGTTATCGAA CTGTCTCGTG ACTGTCTGGA CAAATCTCGT 
GAGGGTCTCA TCACCTCCCG CTACTTCTAT GAGCTACAAG AGAACCTCGA AAAGCTACAC 
CAGGATGCTC ATGAGCGTTC AGAAAGTGGG GAGGTGACAT TTGTCACCCA GCTAGTTAAA 
AAGCTGATGA TAATCATCGC TCGGCCTGCC CGGCTGCTAG AATGTTTGGA GTTTGACCCG 
GAGGAGTTCT ACCACCTGTT AGAGGCAGCT GAAGGTCATG CTAAAGAAGG TCAAGGCATC 
AAGTCTGACA TCCCTCGATA CATCATCAGC CAGCTGGGCC TCACCAGGGA CCCCCTTGAG 
GAAATGGCCC AGCTGAGCAG CTATGACAGC GGTAACCCTG AAACACCAGA GACAGAGACA 
GATTCCACAG ATAGTCGAGG AGCAACCGTA CAGCCACTCC AACCTCAGCC TCAACCGCAG 
CCCAAGACTA AAACACCTCG CGAGGAAGAC TTCGAGACCA TTAAACTCAT CAGTAATGGA 
GCCTATGGGG CTGTGTTTCT GGTGAGGCAT AAAGAGACCC GTCAGCGATT TGCCATGAAG 
AAGATCAACA AGCAGAACCT GATCCTAAGA AACCAGATCC AGCAGGCCTT TGTGGAAAGA 
GACATCTTGA CATTTGCAGA AAACCCTTTT GTTGTCAGCA TGTTCTGCTC CTTTGAGACA 
AGGAGACATC TTTGCATGGT TATGGAGTAT GTGGAGGGTG GGGATTGTGC CACTCTCCTG 
AAGCACATTG GAGCTCTGCC TGTGGATATG GCACGCATGT ACTTTGCTGA AACCGTTCTT 
GCATTGGAAT ACCTTCACAA CTATGGTATT GTGCACAGAG ACCTCAAACC TGACAATCTC 
CTGATTACCT CAATGGGACA CGTCAAACTG ACTGACTTTG GCCTGTCCAA GATTGGTTTA 
ATGAGCTTAA CAACTAATCT GTATGAGGGC CACATTGAAA AAGATACTCG AGAATTCTTG 
GACAAACAGG TGTGTGGTAC TCCTGAATAC ATTGCTCCTG AGGTTATTCT GCGACAGGGT 
TATGGAAAAC CAGTTGACTG GTGGGCCATG GGAGTCATCT TGTATGAGTT TCTGGTGGGC 
TGTGCTCCTT TCTTTGGAGA TACACCTGAA GAACTGTTTG GACAAGTCAT CAGTGATGAG 
ATAATCTGGC CAGAAGGTGA TGAAGCTTTG CCTCCGGATG CTCAAGATCT CATCTCCAAA 
CTTCTTCGGC AAAACCCTCT AGAGCGTCTT GGGACAGGGA GTGCGTTTGA GGTAAAACAG 
CACCGCTTCT TTACTGATCT GGACTGGAAC AGCCTCCTGA GGCAGAAGGC CGAGTTCATT 
CCTCAGCTGG AGTCGGAGGA TGACACTAGC TACTTTGACA CACGCTCAGA TCGTTATCAT 
CATGTGGACT CAGAAGAAGA AGATGACACA AATGATGACG ACCATTTGGA GATCCGTCAG 
TTCTCCTCCT GTTCGCCACG ATTCAGCAAG GTGTATAGTA GTATGGAGCG TCTTTCCTTG 
CATGAGGAGA AACGCACCCC TCCTCCTACT AAGCGCAGTC TGAGTGAAGA GGGAGGAGAG 
CGGATCGACA GTCTAAGTGG CCTAAAGTCC AGAGACCGAA GCTGGTTGGT GGGCTCACCT 
GAAATTCTCA GAAAGCGTCT CTCTGTTTCC GAGTCTTCTC ACACCGAGAG TGACTCGAGT 
CCACCTCTGA CTGTTCGTCG CCGCTGCTGC TCGGCCATCA TTGAAATGCC TCGCTTCGCC 
ATATCCAGTG AAGAGGATAG CTGTGTGGGG AGCAGGAAGA CCCCTGTGAG AGGCCCTCGA 
GGGGATGATC TGCCTCAGGC CATACCAGAG CTCCCTGTAG AGAGAGAGCT CAGGCTGGAT 
GAATCGCCCA CCACGCCAGG ATCCACTTCC AGTCAAATCA GCCAGTCCAC ACTCACACCT 
GGTAGTTCTG GTGATGTGTT AGATCGTGCT TCTCGCTATA GTGCTGAAGT TTCTGAAAAC 
TCGACTCCAA GAGCCATCAG TGATTTGGCG GCTCGAAGAG CTCGCCACAG ACTGCTTTCA 
GGAGATACAG ATAAACACAC ATCACGTCCA CTTAGCAAAG TCATCAAATC AGCTTCAGCA 
ACAACCCTGT CACTCATGAT CCCTGCTGAT CACCATGGTG CTTCTCCACT GGCCAGTCCC 
ATGTCGCCTC ACTCTCTCTC TTCGAACCCC TCATCACGTG ACTCCTCGCC CAGCCGTGAC 
CTTTCCCCAG CTGTCTGTAG TGTTAAACCT GCCATAATCA TCCACCGAGC TGGAAAGAAA 
TATGGTTTTA CTTTACGTGC AATCCGGGTT TACATGGGAG ACACAGACAT ATATACTGTC 
CATCACATGG TCTGGCATGT GGAGGATGGA GGACCTGCTC ATGAGGCTGG ACTGAGAGAG 
GGAGATCTCA TCACACATGT TAACGGAGAG CCTGTCCATG GACTTGTGCA CACAGAAGTT 
GTTGAGCTCA TTCTTAAGAG TGGCAGTAAG GTGTCCATCT CTGCTACACC CTTTGAAAAT 
ACATCAATTA AGGTTGGTCC TGCTCGCAAA ACTAGCTACA AGTCCAAAAT GGCCCGTCGC 
AACAAAAGAA CCAAAACTAG AGAAGGACAA GATAGTAAGA AGAGGAATTC CCTCTTTCGT 
AAGATCACCA AACAGGCGAC CCTTCTTCAC ACCAGTCGCA GCCTGTCCTC TCTGAATCGC 
TCGGTCTCTT CTGGTGAGAG CGTCCCAGGC TCTCCTACTC ACATGTCACC TCATTCGCCC 
ACACAGGGCT GTCGTTCAAC ACCTGACTCC GCCCACTCAG TTGGTGGAAA CTCATCCCAA 
AGTAGCTCGC CTAGCTCCAG TGTGCCAAAC TCTCCTGCCA GTTCTGGCCA GATCAGACCC 
AGCTCCTTGC ATGGTCTGGC CCCCAAGCTG CAGAGACAGT ACCGCTCCCC CCGCCGGAAG 
TCTGCAGGCA ACATCCCTTT ATCCCCTCTG GCTCGTACAC CATCGCCCAC TCCACAGAGC 
ACTTCTCCCC AGCGTTCCCC TTCTCCCCTG CCAAGCCACG GGCTCATCAC TTCTTCAATA 
GGGCAGTCTT TTCCTGTCAA ACTGCACTCT TCACCTCCAT TAGTAAGACA GATATCTCGG 
CCAAAGAGTG CAGACCCTCC TCGCTCTCCT TTGCTAAAAC GAGTCCAGTC CGCTGAGAAA 
CTCGCTGCCT CTCTTTCTTC TTCATCCTCC TCTCCTTCTC CCTCTTCGGC TGCTGATAAA 
AAGCTCCCAG TTGGTGCCAG CCGCAAACAC AGTCTTGATA CCTCACATTC AGAGTTTAAA 
AAAGAGATGC TGCAGCGAGA CCCTAGTCTA CAGAGCCTGC AAGAGTCAGC CAGTGAGACT 
CTTATGGGAG GAAGAGTGTC TCCTGCAGAA AAGGGAAGCC TGCAGAAAAG TTCAGTGCGT 
AAACTGGGCA GGCAAGAAGG TCCGGAGTCT GGAACAGGAA CTCTGGGACT TGTTCCTGGA 
AAGAGTAAAT TGAAAGAAAA GCTGTCTGCC ATACGTGCTG AACGCCGCGA ATCTCTTCAA 
AAGCAGGATG CCATCCATGA AGTGGATTCA TCTGAAGATG AGACAGACGA AGGGTCGGAA 
GACAGCCAAG ATGGGCGCAG AACTTGCTAT ACTCCTCCTA GCCATGTCTT GAGGCCTACA 
ACTGTAATGG CCTCTCCCCA TACCATCAGA ATGGGGCCAG CGGCTCCACC AACACTGGGA 
CTTTTTCCTT CAACAGTTGC ACCGCCAAGC TCACCTCAGC TCATCAGAGG ACTGCAGATA 
CAAAACCAAA GTACCACTCA ATCTCAGACA CAAACTTCAA CCTCACAACA AGCGCAAGCT 
GCAGTTAAAC CCCCAGAACA GAAACCAATA ACCTCTGTTA AAGACTCAAG TTCAACAATA 
ATTGATGACT CGAAAAAACA GTCACAGGCA CAAGAAATTC AGTCCAGACC TTCTCCACAT 
CAACCCAGTC CTCTGTCTAG TACCTTTTCC ACCCAAGGCA GCGCCTTTAC CTCTGTTAGT 
AAAGACCCAT TTGTCAAGCC CACCACACCT CCATTGGATC CCCGGAGGAC CCTAAAAATG 
TCTGAGCCAA GGTCAAAAAC CAGTGGTAGA ACTGATTCTA GCACTACTTC TGGAGTAGCC 
AGGGATACCC CAGTTGCGAC TACCCCAACA GGAGGAATCA CTAAGGAGAT GAAGGAGGCT 
GACAATCGCA GACAGGCTGC AGCTGCTGCT GCTGCCTCTG CCGCTGCTGC TACCACCTCT 
GCATCCACTG CCTCTGAAAG TGGAATGGAT AAAGTTGTGT CTCAGCTCAC CACTGTGGCC 
AAGAGTGTGC TTGGACCTGT CAAGCTCAAT ATCCCAGGTA CTAAAGAGAC CATGGAACGA 
ACAAAGGACC AGCGTACCCA GGCAGATGCC ACCCACCCAA AGATAAAAGA ATGTCGCCTG 
GAGCACCCTG ATTCTCCTGA CCAATTGAGT CCATCTGCTG CTTCGGGCTC CCAGTCTCCA 
CGACTGACTG AATCTCCATC TAAACAAACA TCATCCAAAT CTGAGCCAGC CTCAACTTCT 
CAGAGACCTT TAGAGGTCCC AGGGACTTTC AAAAGACAAA CTTCTCCAAT CCCTCAAGCT 
GGGGAAGAGC ATCAGGATAG GTCGGGAACT CTGCAAAGTG GCACCACATC AAGGCCTAGG 
TCTGAAGCAC AATCACAGAC CCAGCAGGCA AAGCCCAGTT CCACTACGTC ACGAGATAAA 
TCCAACAAGA CAACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_021474608.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330283.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X1, mRNA.

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CloneID	ODa146867
Clone ID Related Accession (Same CDS sequence)	XM_021474608.1
Accession Version	XM_021474608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395380.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACT CCTTCTGGAC CGTTCTCTCC AATTTCTCCC TGCCGGAGAG AAGCATGCTC 
CGCCGATCCA AGAGCTGTCG GACGAGCAAC CGAAAGAGTT TGATAGTGAC ATCCAGCACG 
TCTCCAACGC TGCCTCGACC CCATTCACCA CTGCACGGGC ATCCAGGAAG CAGCCCATTG 
GACAGCCCTC GCAATTTCTC CCCCAACACT GCAGCCCACT TCTCTTTTGT ACCAGCTCGA 
AGCCATGGAC ACAGGGCTGA CAGGACTGAT GGTCGTCGCT GGTCTCTCGC CTCTCTGCCG 
TCCTCTGGCT ATGGAACAAA CACCCCCAGT TCGACAGTCT CTTCATCATG TTCATCTCAG 
GAGAAGCTGC ACCAGTTGCC CTTCCAGCCC ACTGCAGACG AGCTGCATTT CCTCACCAAA 
CACTTCAGCT CGGAGAGCAT CACGGATGAG GATGGGAGAC GATCCCCAGC CATGCGTCCG 
CGCTCCCGCA GTCTCAGCCC TGGACGGTCA CCAATATCAT TTGACCATGA GATTGTCATG 
ATGAACCATG TCTACAAGGA GCGTTTTCCT AAGGCCACTG CTCAGATGGA GGTGCGTCTG 
GCCGATTTCA TATCCTCCTC AGCTCCAGAA AAAGTCATGC CTTTAGCTGA TGGCGTCCTG 
AGCTTCATCC ACCACCAGGT TATCGAACTG TCTCGTGACT GTCTGGACAA ATCTCGTGAG 
GGTCTCATCA CCTCCCGCTA CTTCTATGAG CTACAAGAGA ACCTCGAAAA GCTACACCAG 
GATGCTCATG AGCGTTCAGA AAGTGGGGAG GTGACATTTG TCACCCAGCT AGTTAAAAAG 
CTGATGATAA TCATCGCTCG GCCTGCCCGG CTGCTAGAAT GTTTGGAGTT TGACCCGGAG 
GAGTTCTACC ACCTGTTAGA GGCAGCTGAA GGTCATGCTA AAGAAGGTCA AGGCATCAAG 
TCTGACATCC CTCGATACAT CATCAGCCAG CTGGGCCTCA CCAGGGACCC CCTTGAGGAA 
ATGGCCCAGC TGAGCAGCTA TGACAGCGGT AACCCTGAAA CACCAGAGAC AGAGACAGAT 
TCCACAGATA GTCGAGGAGC AACCGTACAG CCACTCCAAC CTCAGCCTCA ACCGCAGCCC 
AAGACTAAAA CACCTCGCGA GGAAGACTTC GAGACCATTA AACTCATCAG TAATGGAGCC 
TATGGGGCTG TGTTTCTGGT GAGGCATAAA GAGACCCGTC AGCGATTTGC CATGAAGAAG 
ATCAACAAGC AGAACCTGAT CCTAAGAAAC CAGATCCAGC AGGCCTTTGT GGAAAGAGAC 
ATCTTGACAT TTGCAGAAAA CCCTTTTGTT GTCAGCATGT TCTGCTCCTT TGAGACAAGG 
AGACATCTTT GCATGGTTAT GGAGTATGTG GAGGGTGGGG ATTGTGCCAC TCTCCTGAAG 
CACATTGGAG CTCTGCCTGT GGATATGGCA CGCATGTACT TTGCTGAAAC CGTTCTTGCA 
TTGGAATACC TTCACAACTA TGGTATTGTG CACAGAGACC TCAAACCTGA CAATCTCCTG 
ATTACCTCAA TGGGACACGT CAAACTGACT GACTTTGGCC TGTCCAAGAT TGGTTTAATG 
AGCTTAACAA CTAATCTGTA TGAGGGCCAC ATTGAAAAAG ATACTCGAGA ATTCTTGGAC 
AAACAGGTGT GTGGTACTCC TGAATACATT GCTCCTGAGG TTATTCTGCG ACAGGGTTAT 
GGAAAACCAG TTGACTGGTG GGCCATGGGA GTCATCTTGT ATGAGTTTCT GGTGGGCTGT 
GCTCCTTTCT TTGGAGATAC ACCTGAAGAA CTGTTTGGAC AAGTCATCAG TGATGAGATA 
ATCTGGCCAG AAGGTGATGA AGCTTTGCCT CCGGATGCTC AAGATCTCAT CTCCAAACTT 
CTTCGGCAAA ACCCTCTAGA GCGTCTTGGG ACAGGGAGTG CGTTTGAGGT AAAACAGCAC 
CGCTTCTTTA CTGATCTGGA CTGGAACAGC CTCCTGAGGC AGAAGGCCGA GTTCATTCCT 
CAGCTGGAGT CGGAGGATGA CACTAGCTAC TTTGACACAC GCTCAGATCG TTATCATCAT 
GTGGACTCAG AAGAAGAAGA TGACACAAAT GATGACGACC ATTTGGAGAT CCGTCAGTTC 
TCCTCCTGTT CGCCACGATT CAGCAAGGTG TATAGTAGTA TGGAGCGTCT TTCCTTGCAT 
GAGGAGAAAC GCACCCCTCC TCCTACTAAG CGCAGTCTGA GTGAAGAGGG AGGAGAGCGG 
ATCGACAGTC TAAGTGGCCT AAAGTCCAGA GACCGAAGCT GGTTGGTGGG CTCACCTGAA 
ATTCTCAGAA AGCGTCTCTC TGTTTCCGAG TCTTCTCACA CCGAGAGTGA CTCGAGTCCA 
CCTCTGACTG TTCGTCGCCG CTGCTGCTCG GCCATCATTG AAATGCCTCG CTTCGCCATA 
TCCAGTGAAG AGGATAGCTG TGTGGGGAGC AGGAAGACCC CTGTGAGAGG CCCTCGAGGG 
GATGATCTGC CTCAGGCCAT ACCAGAGCTC CCTGTAGAGA GAGAGCTCAG GCTGGATGAA 
TCGCCCACCA CGCCAGGATC CACTTCCAGT CAAATCAGCC AGTCCACACT CACACCTGGT 
AGTTCTGGTG ATGTGTTAGA TCGTGCTTCT CGCTATAGTG CTGAAGTTTC TGAAAACTCG 
ACTCCAAGAG CCATCAGTGA TTTGGCGGCT CGAAGAGCTC GCCACAGACT GCTTTCAGGA 
GATACAGATA AACACACATC ACGTCCACTT AGCAAAGTCA TCAAATCAGC TTCAGCAACA 
ACCCTGTCAC TCATGATCCC TGCTGATCAC CATGGTGCTT CTCCACTGGC CAGTCCCATG 
TCGCCTCACT CTCTCTCTTC GAACCCCTCA TCACGTGACT CCTCGCCCAG CCGTGACCTT 
TCCCCAGCTG TCTGTAGTGT TAAACCTGCC ATAATCATCC ACCGAGCTGG AAAGAAATAT 
GGTTTTACTT TACGTGCAAT CCGGGTTTAC ATGGGAGACA CAGACATATA TACTGTCCAT 
CACATGGTCT GGCATGTGGA GGATGGAGGA CCTGCTCATG AGGCTGGACT GAGAGAGGGA 
GATCTCATCA CACATGTTAA CGGAGAGCCT GTCCATGGAC TTGTGCACAC AGAAGTTGTT 
GAGCTCATTC TTAAGAGTGG CAGTAAGGTG TCCATCTCTG CTACACCCTT TGAAAATACA 
TCAATTAAGG TTGGTCCTGC TCGCAAAACT AGCTACAAGT CCAAAATGGC CCGTCGCAAC 
AAAAGAACCA AAACTAGAGA AGGACAAGAT AGTAAGAAGA GGAATTCCCT CTTTCGTAAG 
ATCACCAAAC AGGCGACCCT TCTTCACACC AGTCGCAGCC TGTCCTCTCT GAATCGCTCG 
GTCTCTTCTG GTGAGAGCGT CCCAGGCTCT CCTACTCACA TGTCACCTCA TTCGCCCACA 
CAGGGCTGTC GTTCAACACC TGACTCCGCC CACTCAGTTG GTGGAAACTC ATCCCAAAGT 
AGCTCGCCTA GCTCCAGTGT GCCAAACTCT CCTGCCAGTT CTGGCCAGAT CAGACCCAGC 
TCCTTGCATG GTCTGGCCCC CAAGCTGCAG AGACAGTACC GCTCCCCCCG CCGGAAGTCT 
GCAGGCAACA TCCCTTTATC CCCTCTGGCT CGTACACCAT CGCCCACTCC ACAGAGCACT 
TCTCCCCAGC GTTCCCCTTC TCCCCTGCCA AGCCACGGGC TCATCACTTC TTCAATAGGG 
CAGTCTTTTC CTGTCAAACT GCACTCTTCA CCTCCATTAG TAAGACAGAT ATCTCGGCCA 
AAGAGTGCAG ACCCTCCTCG CTCTCCTTTG CTAAAACGAG TCCAGTCCGC TGAGAAACTC 
GCTGCCTCTC TTTCTTCTTC ATCCTCCTCT CCTTCTCCCT CTTCGGCTGC TGATAAAAAG 
CTCCCAGTTG GTGCCAGCCG CAAACACAGT CTTGATACCT CACATTCAGA GTTTAAAAAA 
GAGATGCTGC AGCGAGACCC TAGTCTACAG AGCCTGCAAG AGTCAGCCAG TGAGACTCTT 
ATGGGAGGAA GAGTGTCTCC TGCAGAAAAG GGAAGCCTGC AGAAAAGTTC AGTGCGTAAA 
CTGGGCAGGC AAGAAGGTCC GGAGTCTGGA ACAGGAACTC TGGGACTTGT TCCTGGAAAG 
AGTAAATTGA AAGAAAAGCT GTCTGCCATA CGTGCTGAAC GCCGCGAATC TCTTCAAAAG 
CAGGATGCCA TCCATGAAGT GGATTCATCT GAAGATGAGA CAGACGAAGG GTCGGAAGAC 
AGCCAAGATG GGCGCAGAAC TTGCTATACT CCTCCTAGCC ATGTCTTGAG GCCTACAACT 
GTAATGGCCT CTCCCCATAC CATCAGAATG GGGCCAGCGG CTCCACCAAC ACTGGGACTT 
TTTCCTTCAA CAGTTGCACC GCCAAGCTCA CCTCAGCTCA TCAGAGGACT GCAGATACAA 
AACCAAAGTA CCACTCAATC TCAGACACAA ACTTCAACCT CACAACAAGC GCAAGCTGCA 
GTTAAACCCC CAGAACAGAA ACCAATAACC TCTGTTAAAG ACTCAAGTTC AACAATAATT 
GATGACTCGA AAAAACAGTC ACAGGCACAA GAAATTCAGT CCAGACCTTC TCCACATCAA 
CCCAGTCCTC TGTCTAGTAC CTTTTCCACC CAAGGCAGCG CCTTTACCTC TGTTAGTAAA 
GACCCATTTG TCAAGCCCAC CACACCTCCA TTGGATCCCC GGAGGACCCT AAAAATGTCT 
GAGCCAAGGT CAAAAACCAG TGGTAGAACT GATTCTAGCA CTACTTCTGG AGTAGCCAGG 
GATACCCCAG TTGCGACTAC CCCAACAGGA GGAATCACTA AGGAGATGAA GGAGGCTGAC 
AATCGCAGAC AGGCTGCAGC TGCTGCTGCT GCCTCTGCCG CTGCTGCTAC CACCTCTGCA 
TCCACTGCCT CTGAAAGTGG AATGGATAAA GTTGTGTCTC AGCTCACCAC TGTGGCCAAG 
AGTGTGCTTG GACCTGTCAA GCTCAATATC CCAGGTACTA AAGAGACCAT GGAACGAACA 
AAGGACCAGC GTACCCAGGC AGATGCCACC CACCCAAAGA TAAAAGAATG TCGCCTGGAG 
CACCCTGATT CTCCTGACCA ATTGAGTCCA TCTGCTGCTT CGGGCTCCCA GTCTCCACGA 
CTGACTGAAT CTCCATCTAA ACAAACATCA TCCAAATCTG AGCCAGCCTC AACTTCTCAG 
AGACCTTTAG AGGTCCCAGG GACTTTCAAA AGACAAACTT CTCCAATCCC TCAAGCTGGG 
GAAGAGCATC AGGATAGGTC GGGAACTCTG CAAAGTGGCA CCACATCAAG GCCTAGGTCT 
GAAGCACAAT CACAGACCCA GCAGGCAAAG CCCAGTTCCA CTACGTCACG AGATAAATCC 
AACAAGACAA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330283.1
CDS	124..5598
Translation	MSDSFWTVLSNFSLPERSMLRRSKSCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPHSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKEKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYTPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPSSPQLIRGLQIQNQSTTQSQTQTSTSQQAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQEIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTLKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLTTVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAGEEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_021474608.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474608.1

ORF Insert Sequence:

ATGTCTGACT CCTTCTGGAC CGTTCTCTCC AATTTCTCCC TGCCGGAGAG AAGCATGCTC 
CGCCGATCCA AGAGCTGTCG GACGAGCAAC CGAAAGAGTT TGATAGTGAC ATCCAGCACG 
TCTCCAACGC TGCCTCGACC CCATTCACCA CTGCACGGGC ATCCAGGAAG CAGCCCATTG 
GACAGCCCTC GCAATTTCTC CCCCAACACT GCAGCCCACT TCTCTTTTGT ACCAGCTCGA 
AGCCATGGAC ACAGGGCTGA CAGGACTGAT GGTCGTCGCT GGTCTCTCGC CTCTCTGCCG 
TCCTCTGGCT ATGGAACAAA CACCCCCAGT TCGACAGTCT CTTCATCATG TTCATCTCAG 
GAGAAGCTGC ACCAGTTGCC CTTCCAGCCC ACTGCAGACG AGCTGCATTT CCTCACCAAA 
CACTTCAGCT CGGAGAGCAT CACGGATGAG GATGGGAGAC GATCCCCAGC CATGCGTCCG 
CGCTCCCGCA GTCTCAGCCC TGGACGGTCA CCAATATCAT TTGACCATGA GATTGTCATG 
ATGAACCATG TCTACAAGGA GCGTTTTCCT AAGGCCACTG CTCAGATGGA GGTGCGTCTG 
GCCGATTTCA TATCCTCCTC AGCTCCAGAA AAAGTCATGC CTTTAGCTGA TGGCGTCCTG 
AGCTTCATCC ACCACCAGGT TATCGAACTG TCTCGTGACT GTCTGGACAA ATCTCGTGAG 
GGTCTCATCA CCTCCCGCTA CTTCTATGAG CTACAAGAGA ACCTCGAAAA GCTACACCAG 
GATGCTCATG AGCGTTCAGA AAGTGGGGAG GTGACATTTG TCACCCAGCT AGTTAAAAAG 
CTGATGATAA TCATCGCTCG GCCTGCCCGG CTGCTAGAAT GTTTGGAGTT TGACCCGGAG 
GAGTTCTACC ACCTGTTAGA GGCAGCTGAA GGTCATGCTA AAGAAGGTCA AGGCATCAAG 
TCTGACATCC CTCGATACAT CATCAGCCAG CTGGGCCTCA CCAGGGACCC CCTTGAGGAA 
ATGGCCCAGC TGAGCAGCTA TGACAGCGGT AACCCTGAAA CACCAGAGAC AGAGACAGAT 
TCCACAGATA GTCGAGGAGC AACCGTACAG CCACTCCAAC CTCAGCCTCA ACCGCAGCCC 
AAGACTAAAA CACCTCGCGA GGAAGACTTC GAGACCATTA AACTCATCAG TAATGGAGCC 
TATGGGGCTG TGTTTCTGGT GAGGCATAAA GAGACCCGTC AGCGATTTGC CATGAAGAAG 
ATCAACAAGC AGAACCTGAT CCTAAGAAAC CAGATCCAGC AGGCCTTTGT GGAAAGAGAC 
ATCTTGACAT TTGCAGAAAA CCCTTTTGTT GTCAGCATGT TCTGCTCCTT TGAGACAAGG 
AGACATCTTT GCATGGTTAT GGAGTATGTG GAGGGTGGGG ATTGTGCCAC TCTCCTGAAG 
CACATTGGAG CTCTGCCTGT GGATATGGCA CGCATGTACT TTGCTGAAAC CGTTCTTGCA 
TTGGAATACC TTCACAACTA TGGTATTGTG CACAGAGACC TCAAACCTGA CAATCTCCTG 
ATTACCTCAA TGGGACACGT CAAACTGACT GACTTTGGCC TGTCCAAGAT TGGTTTAATG 
AGCTTAACAA CTAATCTGTA TGAGGGCCAC ATTGAAAAAG ATACTCGAGA ATTCTTGGAC 
AAACAGGTGT GTGGTACTCC TGAATACATT GCTCCTGAGG TTATTCTGCG ACAGGGTTAT 
GGAAAACCAG TTGACTGGTG GGCCATGGGA GTCATCTTGT ATGAGTTTCT GGTGGGCTGT 
GCTCCTTTCT TTGGAGATAC ACCTGAAGAA CTGTTTGGAC AAGTCATCAG TGATGAGATA 
ATCTGGCCAG AAGGTGATGA AGCTTTGCCT CCGGATGCTC AAGATCTCAT CTCCAAACTT 
CTTCGGCAAA ACCCTCTAGA GCGTCTTGGG ACAGGGAGTG CGTTTGAGGT AAAACAGCAC 
CGCTTCTTTA CTGATCTGGA CTGGAACAGC CTCCTGAGGC AGAAGGCCGA GTTCATTCCT 
CAGCTGGAGT CGGAGGATGA CACTAGCTAC TTTGACACAC GCTCAGATCG TTATCATCAT 
GTGGACTCAG AAGAAGAAGA TGACACAAAT GATGACGACC ATTTGGAGAT CCGTCAGTTC 
TCCTCCTGTT CGCCACGATT CAGCAAGGTG TATAGTAGTA TGGAGCGTCT TTCCTTGCAT 
GAGGAGAAAC GCACCCCTCC TCCTACTAAG CGCAGTCTGA GTGAAGAGGG AGGAGAGCGG 
ATCGACAGTC TAAGTGGCCT AAAGTCCAGA GACCGAAGCT GGTTGGTGGG CTCACCTGAA 
ATTCTCAGAA AGCGTCTCTC TGTTTCCGAG TCTTCTCACA CCGAGAGTGA CTCGAGTCCA 
CCTCTGACTG TTCGTCGCCG CTGCTGCTCG GCCATCATTG AAATGCCTCG CTTCGCCATA 
TCCAGTGAAG AGGATAGCTG TGTGGGGAGC AGGAAGACCC CTGTGAGAGG CCCTCGAGGG 
GATGATCTGC CTCAGGCCAT ACCAGAGCTC CCTGTAGAGA GAGAGCTCAG GCTGGATGAA 
TCGCCCACCA CGCCAGGATC CACTTCCAGT CAAATCAGCC AGTCCACACT CACACCTGGT 
AGTTCTGGTG ATGTGTTAGA TCGTGCTTCT CGCTATAGTG CTGAAGTTTC TGAAAACTCG 
ACTCCAAGAG CCATCAGTGA TTTGGCGGCT CGAAGAGCTC GCCACAGACT GCTTTCAGGA 
GATACAGATA AACACACATC ACGTCCACTT AGCAAAGTCA TCAAATCAGC TTCAGCAACA 
ACCCTGTCAC TCATGATCCC TGCTGATCAC CATGGTGCTT CTCCACTGGC CAGTCCCATG 
TCGCCTCACT CTCTCTCTTC GAACCCCTCA TCACGTGACT CCTCGCCCAG CCGTGACCTT 
TCCCCAGCTG TCTGTAGTGT TAAACCTGCC ATAATCATCC ACCGAGCTGG AAAGAAATAT 
GGTTTTACTT TACGTGCAAT CCGGGTTTAC ATGGGAGACA CAGACATATA TACTGTCCAT 
CACATGGTCT GGCATGTGGA GGATGGAGGA CCTGCTCATG AGGCTGGACT GAGAGAGGGA 
GATCTCATCA CACATGTTAA CGGAGAGCCT GTCCATGGAC TTGTGCACAC AGAAGTTGTT 
GAGCTCATTC TTAAGAGTGG CAGTAAGGTG TCCATCTCTG CTACACCCTT TGAAAATACA 
TCAATTAAGG TTGGTCCTGC TCGCAAAACT AGCTACAAGT CCAAAATGGC CCGTCGCAAC 
AAAAGAACCA AAACTAGAGA AGGACAAGAT AGTAAGAAGA GGAATTCCCT CTTTCGTAAG 
ATCACCAAAC AGGCGACCCT TCTTCACACC AGTCGCAGCC TGTCCTCTCT GAATCGCTCG 
GTCTCTTCTG GTGAGAGCGT CCCAGGCTCT CCTACTCACA TGTCACCTCA TTCGCCCACA 
CAGGGCTGTC GTTCAACACC TGACTCCGCC CACTCAGTTG GTGGAAACTC ATCCCAAAGT 
AGCTCGCCTA GCTCCAGTGT GCCAAACTCT CCTGCCAGTT CTGGCCAGAT CAGACCCAGC 
TCCTTGCATG GTCTGGCCCC CAAGCTGCAG AGACAGTACC GCTCCCCCCG CCGGAAGTCT 
GCAGGCAACA TCCCTTTATC CCCTCTGGCT CGTACACCAT CGCCCACTCC ACAGAGCACT 
TCTCCCCAGC GTTCCCCTTC TCCCCTGCCA AGCCACGGGC TCATCACTTC TTCAATAGGG 
CAGTCTTTTC CTGTCAAACT GCACTCTTCA CCTCCATTAG TAAGACAGAT ATCTCGGCCA 
AAGAGTGCAG ACCCTCCTCG CTCTCCTTTG CTAAAACGAG TCCAGTCCGC TGAGAAACTC 
GCTGCCTCTC TTTCTTCTTC ATCCTCCTCT CCTTCTCCCT CTTCGGCTGC TGATAAAAAG 
CTCCCAGTTG GTGCCAGCCG CAAACACAGT CTTGATACCT CACATTCAGA GTTTAAAAAA 
GAGATGCTGC AGCGAGACCC TAGTCTACAG AGCCTGCAAG AGTCAGCCAG TGAGACTCTT 
ATGGGAGGAA GAGTGTCTCC TGCAGAAAAG GGAAGCCTGC AGAAAAGTTC AGTGCGTAAA 
CTGGGCAGGC AAGAAGGTCC GGAGTCTGGA ACAGGAACTC TGGGACTTGT TCCTGGAAAG 
AGTAAATTGA AAGAAAAGCT GTCTGCCATA CGTGCTGAAC GCCGCGAATC TCTTCAAAAG 
CAGGATGCCA TCCATGAAGT GGATTCATCT GAAGATGAGA CAGACGAAGG GTCGGAAGAC 
AGCCAAGATG GGCGCAGAAC TTGCTATACT CCTCCTAGCC ATGTCTTGAG GCCTACAACT 
GTAATGGCCT CTCCCCATAC CATCAGAATG GGGCCAGCGG CTCCACCAAC ACTGGGACTT 
TTTCCTTCAA CAGTTGCACC GCCAAGCTCA CCTCAGCTCA TCAGAGGACT GCAGATACAA 
AACCAAAGTA CCACTCAATC TCAGACACAA ACTTCAACCT CACAACAAGC GCAAGCTGCA 
GTTAAACCCC CAGAACAGAA ACCAATAACC TCTGTTAAAG ACTCAAGTTC AACAATAATT 
GATGACTCGA AAAAACAGTC ACAGGCACAA GAAATTCAGT CCAGACCTTC TCCACATCAA 
CCCAGTCCTC TGTCTAGTAC CTTTTCCACC CAAGGCAGCG CCTTTACCTC TGTTAGTAAA 
GACCCATTTG TCAAGCCCAC CACACCTCCA TTGGATCCCC GGAGGACCCT AAAAATGTCT 
GAGCCAAGGT CAAAAACCAG TGGTAGAACT GATTCTAGCA CTACTTCTGG AGTAGCCAGG 
GATACCCCAG TTGCGACTAC CCCAACAGGA GGAATCACTA AGGAGATGAA GGAGGCTGAC 
AATCGCAGAC AGGCTGCAGC TGCTGCTGCT GCCTCTGCCG CTGCTGCTAC CACCTCTGCA 
TCCACTGCCT CTGAAAGTGG AATGGATAAA GTTGTGTCTC AGCTCACCAC TGTGGCCAAG 
AGTGTGCTTG GACCTGTCAA GCTCAATATC CCAGGTACTA AAGAGACCAT GGAACGAACA 
AAGGACCAGC GTACCCAGGC AGATGCCACC CACCCAAAGA TAAAAGAATG TCGCCTGGAG 
CACCCTGATT CTCCTGACCA ATTGAGTCCA TCTGCTGCTT CGGGCTCCCA GTCTCCACGA 
CTGACTGAAT CTCCATCTAA ACAAACATCA TCCAAATCTG AGCCAGCCTC AACTTCTCAG 
AGACCTTTAG AGGTCCCAGG GACTTTCAAA AGACAAACTT CTCCAATCCC TCAAGCTGGG 
GAAGAGCATC AGGATAGGTC GGGAACTCTG CAAAGTGGCA CCACATCAAG GCCTAGGTCT 
GAAGCACAAT CACAGACCCA GCAGGCAAAG CCCAGTTCCA CTACGTCACG AGATAAATCC 
AACAAGACAA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_021474609.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330284.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X2, mRNA.

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CloneID	ODa146868
Clone ID Related Accession (Same CDS sequence)	XM_021474609.1
Accession Version	XM_021474609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395380.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACT CCAGTATATT AAGGCGAAGA GGCCTTCAGA AGGAGCTGAG TCTTCCTAGA 
AGAGGCAGTT TCTGTCGGAC GAGCAACCGA AAGAGTTTGA TAGTGACATC CAGCACGTCT 
CCAACGCTGC CTCGACCCCA TTCACCACTG CACGGGCATC CAGGAAGCAG CCCATTGGAC 
AGCCCTCGCA ATTTCTCCCC CAACACTGCA GCCCACTTCT CTTTTGTACC AGCTCGAAGC 
CATGGACACA GGGCTGACAG GACTGATGGT CGTCGCTGGT CTCTCGCCTC TCTGCCGTCC 
TCTGGCTATG GAACAAACAC CCCCAGTTCG ACAGTCTCTT CATCATGTTC ATCTCAGGAG 
AAGCTGCACC AGTTGCCCTT CCAGCCCACT GCAGACGAGC TGCATTTCCT CACCAAACAC 
TTCAGCTCGG AGAGCATCAC GGATGAGGAT GGGAGACGAT CCCCAGCCAT GCGTCCGCGC 
TCCCGCAGTC TCAGCCCTGG ACGGTCACCA ATATCATTTG ACCATGAGAT TGTCATGATG 
AACCATGTCT ACAAGGAGCG TTTTCCTAAG GCCACTGCTC AGATGGAGGT GCGTCTGGCC 
GATTTCATAT CCTCCTCAGC TCCAGAAAAA GTCATGCCTT TAGCTGATGG CGTCCTGAGC 
TTCATCCACC ACCAGGTTAT CGAACTGTCT CGTGACTGTC TGGACAAATC TCGTGAGGGT 
CTCATCACCT CCCGCTACTT CTATGAGCTA CAAGAGAACC TCGAAAAGCT ACACCAGGAT 
GCTCATGAGC GTTCAGAAAG TGGGGAGGTG ACATTTGTCA CCCAGCTAGT TAAAAAGCTG 
ATGATAATCA TCGCTCGGCC TGCCCGGCTG CTAGAATGTT TGGAGTTTGA CCCGGAGGAG 
TTCTACCACC TGTTAGAGGC AGCTGAAGGT CATGCTAAAG AAGGTCAAGG CATCAAGTCT 
GACATCCCTC GATACATCAT CAGCCAGCTG GGCCTCACCA GGGACCCCCT TGAGGAAATG 
GCCCAGCTGA GCAGCTATGA CAGCGGTAAC CCTGAAACAC CAGAGACAGA GACAGATTCC 
ACAGATAGTC GAGGAGCAAC CGTACAGCCA CTCCAACCTC AGCCTCAACC GCAGCCCAAG 
ACTAAAACAC CTCGCGAGGA AGACTTCGAG ACCATTAAAC TCATCAGTAA TGGAGCCTAT 
GGGGCTGTGT TTCTGGTGAG GCATAAAGAG ACCCGTCAGC GATTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTGATCCT AAGAAACCAG ATCCAGCAGG CCTTTGTGGA AAGAGACATC 
TTGACATTTG CAGAAAACCC TTTTGTTGTC AGCATGTTCT GCTCCTTTGA GACAAGGAGA 
CATCTTTGCA TGGTTATGGA GTATGTGGAG GGTGGGGATT GTGCCACTCT CCTGAAGCAC 
ATTGGAGCTC TGCCTGTGGA TATGGCACGC ATGTACTTTG CTGAAACCGT TCTTGCATTG 
GAATACCTTC ACAACTATGG TATTGTGCAC AGAGACCTCA AACCTGACAA TCTCCTGATT 
ACCTCAATGG GACACGTCAA ACTGACTGAC TTTGGCCTGT CCAAGATTGG TTTAATGAGC 
TTAACAACTA ATCTGTATGA GGGCCACATT GAAAAAGATA CTCGAGAATT CTTGGACAAA 
CAGGTGTGTG GTACTCCTGA ATACATTGCT CCTGAGGTTA TTCTGCGACA GGGTTATGGA 
AAACCAGTTG ACTGGTGGGC CATGGGAGTC ATCTTGTATG AGTTTCTGGT GGGCTGTGCT 
CCTTTCTTTG GAGATACACC TGAAGAACTG TTTGGACAAG TCATCAGTGA TGAGATAATC 
TGGCCAGAAG GTGATGAAGC TTTGCCTCCG GATGCTCAAG ATCTCATCTC CAAACTTCTT 
CGGCAAAACC CTCTAGAGCG TCTTGGGACA GGGAGTGCGT TTGAGGTAAA ACAGCACCGC 
TTCTTTACTG ATCTGGACTG GAACAGCCTC CTGAGGCAGA AGGCCGAGTT CATTCCTCAG 
CTGGAGTCGG AGGATGACAC TAGCTACTTT GACACACGCT CAGATCGTTA TCATCATGTG 
GACTCAGAAG AAGAAGATGA CACAAATGAT GACGACCATT TGGAGATCCG TCAGTTCTCC 
TCCTGTTCGC CACGATTCAG CAAGGTGTAT AGTAGTATGG AGCGTCTTTC CTTGCATGAG 
GAGAAACGCA CCCCTCCTCC TACTAAGCGC AGTCTGAGTG AAGAGGGAGG AGAGCGGATC 
GACAGTCTAA GTGGCCTAAA GTCCAGAGAC CGAAGCTGGT TGGTGGGCTC ACCTGAAATT 
CTCAGAAAGC GTCTCTCTGT TTCCGAGTCT TCTCACACCG AGAGTGACTC GAGTCCACCT 
CTGACTGTTC GTCGCCGCTG CTGCTCGGCC ATCATTGAAA TGCCTCGCTT CGCCATATCC 
AGTGAAGAGG ATAGCTGTGT GGGGAGCAGG AAGACCCCTG TGAGAGGCCC TCGAGGGGAT 
GATCTGCCTC AGGCCATACC AGAGCTCCCT GTAGAGAGAG AGCTCAGGCT GGATGAATCG 
CCCACCACGC CAGGATCCAC TTCCAGTCAA ATCAGCCAGT CCACACTCAC ACCTGGTAGT 
TCTGGTGATG TGTTAGATCG TGCTTCTCGC TATAGTGCTG AAGTTTCTGA AAACTCGACT 
CCAAGAGCCA TCAGTGATTT GGCGGCTCGA AGAGCTCGCC ACAGACTGCT TTCAGGAGAT 
ACAGATAAAC ACACATCACG TCCACTTAGC AAAGTCATCA AATCAGCTTC AGCAACAACC 
CTGTCACTCA TGATCCCTGC TGATCACCAT GGTGCTTCTC CACTGGCCAG TCCCATGTCG 
CCTCACTCTC TCTCTTCGAA CCCCTCATCA CGTGACTCCT CGCCCAGCCG TGACCTTTCC 
CCAGCTGTCT GTAGTGTTAA ACCTGCCATA ATCATCCACC GAGCTGGAAA GAAATATGGT 
TTTACTTTAC GTGCAATCCG GGTTTACATG GGAGACACAG ACATATATAC TGTCCATCAC 
ATGGTCTGGC ATGTGGAGGA TGGAGGACCT GCTCATGAGG CTGGACTGAG AGAGGGAGAT 
CTCATCACAC ATGTTAACGG AGAGCCTGTC CATGGACTTG TGCACACAGA AGTTGTTGAG 
CTCATTCTTA AGAGTGGCAG TAAGGTGTCC ATCTCTGCTA CACCCTTTGA AAATACATCA 
ATTAAGGTTG GTCCTGCTCG CAAAACTAGC TACAAGTCCA AAATGGCCCG TCGCAACAAA 
AGAACCAAAA CTAGAGAAGG ACAAGATAGT AAGAAGAGGA ATTCCCTCTT TCGTAAGATC 
ACCAAACAGG CGACCCTTCT TCACACCAGT CGCAGCCTGT CCTCTCTGAA TCGCTCGGTC 
TCTTCTGGTG AGAGCGTCCC AGGCTCTCCT ACTCACATGT CACCTCATTC GCCCACACAG 
GGCTGTCGTT CAACACCTGA CTCCGCCCAC TCAGTTGGTG GAAACTCATC CCAAAGTAGC 
TCGCCTAGCT CCAGTGTGCC AAACTCTCCT GCCAGTTCTG GCCAGATCAG ACCCAGCTCC 
TTGCATGGTC TGGCCCCCAA GCTGCAGAGA CAGTACCGCT CCCCCCGCCG GAAGTCTGCA 
GGCAACATCC CTTTATCCCC TCTGGCTCGT ACACCATCGC CCACTCCACA GAGCACTTCT 
CCCCAGCGTT CCCCTTCTCC CCTGCCAAGC CACGGGCTCA TCACTTCTTC AATAGGGCAG 
TCTTTTCCTG TCAAACTGCA CTCTTCACCT CCATTAGTAA GACAGATATC TCGGCCAAAG 
AGTGCAGACC CTCCTCGCTC TCCTTTGCTA AAACGAGTCC AGTCCGCTGA GAAACTCGCT 
GCCTCTCTTT CTTCTTCATC CTCCTCTCCT TCTCCCTCTT CGGCTGCTGA TAAAAAGCTC 
CCAGTTGGTG CCAGCCGCAA ACACAGTCTT GATACCTCAC ATTCAGAGTT TAAAAAAGAG 
ATGCTGCAGC GAGACCCTAG TCTACAGAGC CTGCAAGAGT CAGCCAGTGA GACTCTTATG 
GGAGGAAGAG TGTCTCCTGC AGAAAAGGGA AGCCTGCAGA AAAGTTCAGT GCGTAAACTG 
GGCAGGCAAG AAGGTCCGGA GTCTGGAACA GGAACTCTGG GACTTGTTCC TGGAAAGAGT 
AAATTGAAAG AAAAGCTGTC TGCCATACGT GCTGAACGCC GCGAATCTCT TCAAAAGCAG 
GATGCCATCC ATGAAGTGGA TTCATCTGAA GATGAGACAG ACGAAGGGTC GGAAGACAGC 
CAAGATGGGC GCAGAACTTG CTATACTCCT CCTAGCCATG TCTTGAGGCC TACAACTGTA 
ATGGCCTCTC CCCATACCAT CAGAATGGGG CCAGCGGCTC CACCAACACT GGGACTTTTT 
CCTTCAACAG TTGCACCGCC AAGCTCACCT CAGCTCATCA GAGGACTGCA GATACAAAAC 
CAAAGTACCA CTCAATCTCA GACACAAACT TCAACCTCAC AACAAGCGCA AGCTGCAGTT 
AAACCCCCAG AACAGAAACC AATAACCTCT GTTAAAGACT CAAGTTCAAC AATAATTGAT 
GACTCGAAAA AACAGTCACA GGCACAAGAA ATTCAGTCCA GACCTTCTCC ACATCAACCC 
AGTCCTCTGT CTAGTACCTT TTCCACCCAA GGCAGCGCCT TTACCTCTGT TAGTAAAGAC 
CCATTTGTCA AGCCCACCAC ACCTCCATTG GATCCCCGGA GGACCCTAAA AATGTCTGAG 
CCAAGGTCAA AAACCAGTGG TAGAACTGAT TCTAGCACTA CTTCTGGAGT AGCCAGGGAT 
ACCCCAGTTG CGACTACCCC AACAGGAGGA ATCACTAAGG AGATGAAGGA GGCTGACAAT 
CGCAGACAGG CTGCAGCTGC TGCTGCTGCC TCTGCCGCTG CTGCTACCAC CTCTGCATCC 
ACTGCCTCTG AAAGTGGAAT GGATAAAGTT GTGTCTCAGC TCACCACTGT GGCCAAGAGT 
GTGCTTGGAC CTGTCAAGCT CAATATCCCA GGTACTAAAG AGACCATGGA ACGAACAAAG 
GACCAGCGTA CCCAGGCAGA TGCCACCCAC CCAAAGATAA AAGAATGTCG CCTGGAGCAC 
CCTGATTCTC CTGACCAATT GAGTCCATCT GCTGCTTCGG GCTCCCAGTC TCCACGACTG 
ACTGAATCTC CATCTAAACA AACATCATCC AAATCTGAGC CAGCCTCAAC TTCTCAGAGA 
CCTTTAGAGG TCCCAGGGAC TTTCAAAAGA CAAACTTCTC CAATCCCTCA AGCTGGGGAA 
GAGCATCAGG ATAGGTCGGG AACTCTGCAA AGTGGCACCA CATCAAGGCC TAGGTCTGAA 
GCACAATCAC AGACCCAGCA GGCAAAGCCC AGTTCCACTA CGTCACGAGA TAAATCCAAC 
AAGACAACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330284.1
CDS	1616..7087
Translation	MDDSSILRRRGLQKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPHSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKEKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYTPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPSSPQLIRGLQIQNQSTTQSQTQTSTSQQAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQEIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTLKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLTTVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAGEEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_021474609.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474609.1

ORF Insert Sequence:

ATGGATGACT CCAGTATATT AAGGCGAAGA GGCCTTCAGA AGGAGCTGAG TCTTCCTAGA 
AGAGGCAGTT TCTGTCGGAC GAGCAACCGA AAGAGTTTGA TAGTGACATC CAGCACGTCT 
CCAACGCTGC CTCGACCCCA TTCACCACTG CACGGGCATC CAGGAAGCAG CCCATTGGAC 
AGCCCTCGCA ATTTCTCCCC CAACACTGCA GCCCACTTCT CTTTTGTACC AGCTCGAAGC 
CATGGACACA GGGCTGACAG GACTGATGGT CGTCGCTGGT CTCTCGCCTC TCTGCCGTCC 
TCTGGCTATG GAACAAACAC CCCCAGTTCG ACAGTCTCTT CATCATGTTC ATCTCAGGAG 
AAGCTGCACC AGTTGCCCTT CCAGCCCACT GCAGACGAGC TGCATTTCCT CACCAAACAC 
TTCAGCTCGG AGAGCATCAC GGATGAGGAT GGGAGACGAT CCCCAGCCAT GCGTCCGCGC 
TCCCGCAGTC TCAGCCCTGG ACGGTCACCA ATATCATTTG ACCATGAGAT TGTCATGATG 
AACCATGTCT ACAAGGAGCG TTTTCCTAAG GCCACTGCTC AGATGGAGGT GCGTCTGGCC 
GATTTCATAT CCTCCTCAGC TCCAGAAAAA GTCATGCCTT TAGCTGATGG CGTCCTGAGC 
TTCATCCACC ACCAGGTTAT CGAACTGTCT CGTGACTGTC TGGACAAATC TCGTGAGGGT 
CTCATCACCT CCCGCTACTT CTATGAGCTA CAAGAGAACC TCGAAAAGCT ACACCAGGAT 
GCTCATGAGC GTTCAGAAAG TGGGGAGGTG ACATTTGTCA CCCAGCTAGT TAAAAAGCTG 
ATGATAATCA TCGCTCGGCC TGCCCGGCTG CTAGAATGTT TGGAGTTTGA CCCGGAGGAG 
TTCTACCACC TGTTAGAGGC AGCTGAAGGT CATGCTAAAG AAGGTCAAGG CATCAAGTCT 
GACATCCCTC GATACATCAT CAGCCAGCTG GGCCTCACCA GGGACCCCCT TGAGGAAATG 
GCCCAGCTGA GCAGCTATGA CAGCGGTAAC CCTGAAACAC CAGAGACAGA GACAGATTCC 
ACAGATAGTC GAGGAGCAAC CGTACAGCCA CTCCAACCTC AGCCTCAACC GCAGCCCAAG 
ACTAAAACAC CTCGCGAGGA AGACTTCGAG ACCATTAAAC TCATCAGTAA TGGAGCCTAT 
GGGGCTGTGT TTCTGGTGAG GCATAAAGAG ACCCGTCAGC GATTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTGATCCT AAGAAACCAG ATCCAGCAGG CCTTTGTGGA AAGAGACATC 
TTGACATTTG CAGAAAACCC TTTTGTTGTC AGCATGTTCT GCTCCTTTGA GACAAGGAGA 
CATCTTTGCA TGGTTATGGA GTATGTGGAG GGTGGGGATT GTGCCACTCT CCTGAAGCAC 
ATTGGAGCTC TGCCTGTGGA TATGGCACGC ATGTACTTTG CTGAAACCGT TCTTGCATTG 
GAATACCTTC ACAACTATGG TATTGTGCAC AGAGACCTCA AACCTGACAA TCTCCTGATT 
ACCTCAATGG GACACGTCAA ACTGACTGAC TTTGGCCTGT CCAAGATTGG TTTAATGAGC 
TTAACAACTA ATCTGTATGA GGGCCACATT GAAAAAGATA CTCGAGAATT CTTGGACAAA 
CAGGTGTGTG GTACTCCTGA ATACATTGCT CCTGAGGTTA TTCTGCGACA GGGTTATGGA 
AAACCAGTTG ACTGGTGGGC CATGGGAGTC ATCTTGTATG AGTTTCTGGT GGGCTGTGCT 
CCTTTCTTTG GAGATACACC TGAAGAACTG TTTGGACAAG TCATCAGTGA TGAGATAATC 
TGGCCAGAAG GTGATGAAGC TTTGCCTCCG GATGCTCAAG ATCTCATCTC CAAACTTCTT 
CGGCAAAACC CTCTAGAGCG TCTTGGGACA GGGAGTGCGT TTGAGGTAAA ACAGCACCGC 
TTCTTTACTG ATCTGGACTG GAACAGCCTC CTGAGGCAGA AGGCCGAGTT CATTCCTCAG 
CTGGAGTCGG AGGATGACAC TAGCTACTTT GACACACGCT CAGATCGTTA TCATCATGTG 
GACTCAGAAG AAGAAGATGA CACAAATGAT GACGACCATT TGGAGATCCG TCAGTTCTCC 
TCCTGTTCGC CACGATTCAG CAAGGTGTAT AGTAGTATGG AGCGTCTTTC CTTGCATGAG 
GAGAAACGCA CCCCTCCTCC TACTAAGCGC AGTCTGAGTG AAGAGGGAGG AGAGCGGATC 
GACAGTCTAA GTGGCCTAAA GTCCAGAGAC CGAAGCTGGT TGGTGGGCTC ACCTGAAATT 
CTCAGAAAGC GTCTCTCTGT TTCCGAGTCT TCTCACACCG AGAGTGACTC GAGTCCACCT 
CTGACTGTTC GTCGCCGCTG CTGCTCGGCC ATCATTGAAA TGCCTCGCTT CGCCATATCC 
AGTGAAGAGG ATAGCTGTGT GGGGAGCAGG AAGACCCCTG TGAGAGGCCC TCGAGGGGAT 
GATCTGCCTC AGGCCATACC AGAGCTCCCT GTAGAGAGAG AGCTCAGGCT GGATGAATCG 
CCCACCACGC CAGGATCCAC TTCCAGTCAA ATCAGCCAGT CCACACTCAC ACCTGGTAGT 
TCTGGTGATG TGTTAGATCG TGCTTCTCGC TATAGTGCTG AAGTTTCTGA AAACTCGACT 
CCAAGAGCCA TCAGTGATTT GGCGGCTCGA AGAGCTCGCC ACAGACTGCT TTCAGGAGAT 
ACAGATAAAC ACACATCACG TCCACTTAGC AAAGTCATCA AATCAGCTTC AGCAACAACC 
CTGTCACTCA TGATCCCTGC TGATCACCAT GGTGCTTCTC CACTGGCCAG TCCCATGTCG 
CCTCACTCTC TCTCTTCGAA CCCCTCATCA CGTGACTCCT CGCCCAGCCG TGACCTTTCC 
CCAGCTGTCT GTAGTGTTAA ACCTGCCATA ATCATCCACC GAGCTGGAAA GAAATATGGT 
TTTACTTTAC GTGCAATCCG GGTTTACATG GGAGACACAG ACATATATAC TGTCCATCAC 
ATGGTCTGGC ATGTGGAGGA TGGAGGACCT GCTCATGAGG CTGGACTGAG AGAGGGAGAT 
CTCATCACAC ATGTTAACGG AGAGCCTGTC CATGGACTTG TGCACACAGA AGTTGTTGAG 
CTCATTCTTA AGAGTGGCAG TAAGGTGTCC ATCTCTGCTA CACCCTTTGA AAATACATCA 
ATTAAGGTTG GTCCTGCTCG CAAAACTAGC TACAAGTCCA AAATGGCCCG TCGCAACAAA 
AGAACCAAAA CTAGAGAAGG ACAAGATAGT AAGAAGAGGA ATTCCCTCTT TCGTAAGATC 
ACCAAACAGG CGACCCTTCT TCACACCAGT CGCAGCCTGT CCTCTCTGAA TCGCTCGGTC 
TCTTCTGGTG AGAGCGTCCC AGGCTCTCCT ACTCACATGT CACCTCATTC GCCCACACAG 
GGCTGTCGTT CAACACCTGA CTCCGCCCAC TCAGTTGGTG GAAACTCATC CCAAAGTAGC 
TCGCCTAGCT CCAGTGTGCC AAACTCTCCT GCCAGTTCTG GCCAGATCAG ACCCAGCTCC 
TTGCATGGTC TGGCCCCCAA GCTGCAGAGA CAGTACCGCT CCCCCCGCCG GAAGTCTGCA 
GGCAACATCC CTTTATCCCC TCTGGCTCGT ACACCATCGC CCACTCCACA GAGCACTTCT 
CCCCAGCGTT CCCCTTCTCC CCTGCCAAGC CACGGGCTCA TCACTTCTTC AATAGGGCAG 
TCTTTTCCTG TCAAACTGCA CTCTTCACCT CCATTAGTAA GACAGATATC TCGGCCAAAG 
AGTGCAGACC CTCCTCGCTC TCCTTTGCTA AAACGAGTCC AGTCCGCTGA GAAACTCGCT 
GCCTCTCTTT CTTCTTCATC CTCCTCTCCT TCTCCCTCTT CGGCTGCTGA TAAAAAGCTC 
CCAGTTGGTG CCAGCCGCAA ACACAGTCTT GATACCTCAC ATTCAGAGTT TAAAAAAGAG 
ATGCTGCAGC GAGACCCTAG TCTACAGAGC CTGCAAGAGT CAGCCAGTGA GACTCTTATG 
GGAGGAAGAG TGTCTCCTGC AGAAAAGGGA AGCCTGCAGA AAAGTTCAGT GCGTAAACTG 
GGCAGGCAAG AAGGTCCGGA GTCTGGAACA GGAACTCTGG GACTTGTTCC TGGAAAGAGT 
AAATTGAAAG AAAAGCTGTC TGCCATACGT GCTGAACGCC GCGAATCTCT TCAAAAGCAG 
GATGCCATCC ATGAAGTGGA TTCATCTGAA GATGAGACAG ACGAAGGGTC GGAAGACAGC 
CAAGATGGGC GCAGAACTTG CTATACTCCT CCTAGCCATG TCTTGAGGCC TACAACTGTA 
ATGGCCTCTC CCCATACCAT CAGAATGGGG CCAGCGGCTC CACCAACACT GGGACTTTTT 
CCTTCAACAG TTGCACCGCC AAGCTCACCT CAGCTCATCA GAGGACTGCA GATACAAAAC 
CAAAGTACCA CTCAATCTCA GACACAAACT TCAACCTCAC AACAAGCGCA AGCTGCAGTT 
AAACCCCCAG AACAGAAACC AATAACCTCT GTTAAAGACT CAAGTTCAAC AATAATTGAT 
GACTCGAAAA AACAGTCACA GGCACAAGAA ATTCAGTCCA GACCTTCTCC ACATCAACCC 
AGTCCTCTGT CTAGTACCTT TTCCACCCAA GGCAGCGCCT TTACCTCTGT TAGTAAAGAC 
CCATTTGTCA AGCCCACCAC ACCTCCATTG GATCCCCGGA GGACCCTAAA AATGTCTGAG 
CCAAGGTCAA AAACCAGTGG TAGAACTGAT TCTAGCACTA CTTCTGGAGT AGCCAGGGAT 
ACCCCAGTTG CGACTACCCC AACAGGAGGA ATCACTAAGG AGATGAAGGA GGCTGACAAT 
CGCAGACAGG CTGCAGCTGC TGCTGCTGCC TCTGCCGCTG CTGCTACCAC CTCTGCATCC 
ACTGCCTCTG AAAGTGGAAT GGATAAAGTT GTGTCTCAGC TCACCACTGT GGCCAAGAGT 
GTGCTTGGAC CTGTCAAGCT CAATATCCCA GGTACTAAAG AGACCATGGA ACGAACAAAG 
GACCAGCGTA CCCAGGCAGA TGCCACCCAC CCAAAGATAA AAGAATGTCG CCTGGAGCAC 
CCTGATTCTC CTGACCAATT GAGTCCATCT GCTGCTTCGG GCTCCCAGTC TCCACGACTG 
ACTGAATCTC CATCTAAACA AACATCATCC AAATCTGAGC CAGCCTCAAC TTCTCAGAGA 
CCTTTAGAGG TCCCAGGGAC TTTCAAAAGA CAAACTTCTC CAATCCCTCA AGCTGGGGAA 
GAGCATCAGG ATAGGTCGGG AACTCTGCAA AGTGGCACCA CATCAAGGCC TAGGTCTGAA 
GCACAATCAC AGACCCAGCA GGCAAAGCCC AGTTCCACTA CGTCACGAGA TAAATCCAAC 
AAGACAACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_001341269.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001341305.4 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X3, mRNA.

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CloneID	ODa11957
Clone ID Related Accession (Same CDS sequence)	XM_001341269.7 , XM_001341269.8
Accession Version	XM_001341269.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001341269.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAA CCGAGAGCAA AAACAAAAGG AAACTCTGCA CATCTGAGCA GCAGGACAGC 
AGCACTGAAG GAAAAGACGA GGAGCTGCCG GCGCCGCCTC CCCTGCTGTT CAGGAAGCTG 
AGTAACCCAG ACCTGTCTCC TGCCGCAGGA AAAACCAAAC TGCAGAGGCA GCTCAGTCAG 
GATGACAGCA GAGGCCGACG CAGCAGCATG GCTGGCATCC TCACTGGAAA GCAGCTTCTG 
CCTCTGTCCA GCAGTATGCA TGGAGGAGTG AGTCAGCTGG CCTGGCAGCA GCACACAGGG 
GAGCCCAATA ACTTGGTCCG CATGAGGAGT CAGTCCTTTG GCCAGTCTGC CCCGTCTCTC 
ACTGCGGGAC TGAAGGAGCT GAGTCTTCCT AGAAGAGGCA GTTTCTGTCG GACGAGCAAC 
CGAAAAAGTT TGATAGTGAC ATCCAGCACG TCTCCAACGC TGCCTCGACC CCATTCACCA 
CTGCACGGGC ATCCAGGAAG CAGCCCATTG GACAGCCCTC GCAATTTCTC CCCCAACACT 
GCAGCCCACT TCTCTTTTGT ACCAGCTCGA AGGACTGACG GTCGTCGCTG GTCTCTCGCC 
TCTCTGCCGT CCTCTGGCTA TGGAACAAAC ACCCCCAGTT CGACAGTCTC GTCATCATGT 
TCATCTCAGG AGAAGCTGCA CCAGTTGCCC TTCCAGCCCA CTGCAGACGA GCTGCATTTC 
CTCACCAAAC ACTTCAGCTC GGAGAGCATC ACGGATGAGG ATGGGAGACG ATCCCCAGCC 
ATGCGTCCGC GCTCCCGCAG TCTCAGCCCT GGACGGTCAC CAATATCATT TGACCATGAG 
ATTGTCATGA TGAACCATGT CTACAAGGAG CGTTTTCCTA AGGCCACTGC TCAGATGGAG 
GTGCGTCTGG CCGATTTCAT ATCCTCCTCA GCTCCAGAAA AAGTCATGCC TTTAGCTGAT 
GGCGTCCTGA GCTTCATCCA CCACCAGGTT ATCGAACTGT CTCGAGATTG TCTGGACAAA 
TCTCGTGAGG GTCTCATCAC CTCCCGCTAC TTCTATGAGC TACAAGAGAA CCTTGAAAAG 
CTACACCAGG ATGCTCATGA GCGTTCAGAA AGTGGGGAGG TGACATTTGT CACCCAGCTA 
GTTAAAAAGC TGATGATAAT CATCGCTCGG CCTGCCCGGC TGCTAGAATG TTTGGAGTTT 
GACCCGGAGG AGTTCTACCA CCTGTTAGAG GCAGCTGAAG GTCATGCTAA AGAAGGTCAA 
GGCATCAAGT CTGACATCCC TCGATACATC ATCAGCCAGC TGGGCCTCAC CAGGGACCCC 
CTTGAGGAAA TGGCCCAGCT GAGCAGCTAT GACAGCGGTA ACCCTGAAAC ACCAGAGACA 
GAGACAGATT CCACAGATAG TCGAGGAGCA ACCGTACAGC CACTCCAACC TCAGCCTCAA 
CCGCAGCCCA AGACTAAAAC ACCTCGCGAG GAAGACTTCG AGACCATTAA ACTCATCAGT 
AATGGAGCCT ATGGGGCTGT GTTTCTGGTG AGGCATAAAG AGACCCGTCA GCGATTTGCC 
ATGAAGAAGA TCAACAAGCA GAACCTGATC CTAAGAAACC AGATCCAGCA GGCCTTTGTG 
GAAAGAGACA TCTTGACATT TGCAGAAAAC CCTTTTGTTG TCAGCATGTT CTGCTCCTTT 
GAGACAAGGA GACATCTTTG CATGGTTATG GAGTATGTGG AGGGTGGGGA TTGTGCCACT 
CTCCTGAAGC ACATTGGAGC TCTGCCTGTG GATATGGCAC GCATGTACTT TGCTGAAACC 
GTTCTTGCAT TGGAATACCT TCACAACTAT GGTATTGTGC ACAGAGACCT CAAACCTGAC 
AATCTCCTGA TTACCTCAAT GGGACACGTC AAACTGACTG ACTTTGGCCT GTCCAAGATT 
GGTTTAATGA GCTTAACAAC TAATCTGTAT GAGGGCCACA TTGAAAAAGA TACTCGAGAA 
TTCTTGGACA AACAGGTGTG TGGTACTCCT GAATACATTG CTCCTGAGGT TATTCTGCGA 
CAGGGTTATG GAAAACCAGT TGACTGGTGG GCCATGGGAG TCATCTTGTA TGAGTTTCTG 
GTGGGCTGTG CTCCTTTCTT TGGAGATACA CCTGAAGAAC TGTTTGGACA AGTCATCAGT 
GATGAGATAA TCTGGCCAGA AGGTGATGAA GCTTTGCCTC CGGATGCTCA AGATCTCATC 
TCCAAACTTC TTCGGCAAAA CCCTCTAGAG CGTCTTGGGA CAGGGAGTGC GTTTGAGGTA 
AAACAGCACC GCTTCTTTAC TGATCTGGAC TGGAACAGCC TCCTGAGGCA GAAGGCTGAG 
TTCATTCCTC AGCTGGAGTC GGAGGATGAC ACTAGCTACT TTGACACACG CTCAGATCGT 
TATCATCATG TGGACTCAGA GGAAGAAGAT GACACAAATG ATGACGACCA TTTGGAGATC 
CGTCAGTTCT CCTCCTGTTC GCCACGATTC AGCAAGGTGT ATAGTAGTAT GGAGCGTCTT 
TCCTTGCATG AGGAGAAACG CACCCCTCCT CCTACTAAGC GCAGTCTGAG TGAAGAGGGA 
GGAGAGCGGA TCGACAGTCT AAGTGGCCTA AAGTCCAGAG ACCGAAGCTG GTTGGTGGGC 
TCACCTGAAA TTCTCAGAAA GCGTCTCTCT GTTTCCGAGT CTTCTCACAC CGAGAGTGAC 
TCGAGTCCAC CTCTGACTGT TCGTCGCCGC TGCTGCTCGG CCATCATTGA AATGCCTCGC 
TTCGCCATAT CCAGTGAAGA GGATAGCTGT GTGGGGAGCA GGAAGACCCC TGTGAGAGGC 
CCTCGAGGGG ATGATCTGCC TCAGGCCATA CCAGAGCTCC CTGTAGAGAG AGAGCTCAGG 
CTGGATGAAT CGCCCACCAC GCCAGGATCC ACTTCCAGTC AAATCAGCCA GTCCACACTC 
ACACCTGGTA GTTCTGGTGA TGTGTTAGAT CGTGCTTCTC GCTATAGTGC TGAAGTTTCT 
GAAAACTCCA CTCCAAGAGC CATCAGTGAT TTGGCGGCTC GAAGAGCTCG CCACAGACTG 
CTTTCAGGAG ATACAGATAA ACACACATCA CGTCCACTTA GCAAAGTCAT CAAATCAGCT 
TCAGCAACAA CCCTGTCACT CATGATCCCT GCTGATCACC ATGGTGCTTC TCCACTGGCC 
AGTCCCATGT CGCCTCACTC TCTCTCTTCG AACCCCTCAT CACGTGACTC CTCGCCCAGC 
CGTGACCTTT CCCCAGCTGT CTGTAGTGTT AAACCTGCCA TAATCATCCA TCGAGCTGGA 
AAGAAATATG GTTTTACTTT ACGTGCAATC CGGGTTTACA TGGGAGACAC AGACATATAT 
ACTGTCCATC ACATGGTCTG GCATGTGGAG GATGGAGGAC CTGCTCATGA GGCTGGACTG 
AGAGAGGGAG ATCTCATCAC ACATGTTAAC GGAGAGCCTG TCCATGGACT TGTGCACACA 
GAAGTTGTTG AGCTCATTCT TAAGAGTGGC AGTAAGGTGT CCATCTCTGC TACACCCTTT 
GAAAATACAT CAATTAAGGT TGGTCCTGCT CGCAAAACTA GCTACAAGTC CAAAATGGCC 
CGTCGCAACA AAAGAACCAA AACTAGAGAA GGACAAGACA GTAAGAAGAG GAATTCCCTC 
TTCCGTAAGA TCACCAAACA GGCGACCCTT CTTCACACCA GTCGCAGCCT GTCCTCTCTG 
AATCGCTCGG TCTCTTCTGG TGAGAGCGTC CCAGGCTCTC CTACACACAT GTCACCTCGT 
TCACCCACAC AGGGCTGTCG TTCAACACCT GACTCCGCCC ACTCAGTTGG TGGAAACTCA 
TCCCAAAGTA GCTCGCCTAG CTCCAGTGTG CCAAACTCTC CTGCCAGCTC TGGCCAGATC 
AGACCCAGCT CCTTGCATGG TCTGGCCCCC AAGCTGCAGC GCCAGTACCG ATCCCCCCGC 
CGGAAGTCTG CAGGCAACAT CCCTTTATCC CCTCTGGCCC GTACACCATC GCCCACTCCA 
CAGAGCACTT CTCCCCAGCG TTCCCCTTCT CCCCTGCCAA GCCACGGGCT CATCACTTCT 
TCAATAGGCC AGTCTTTTCC TGTCAAACTG CACTCTTCAC CTCCATTAGT AAGACAGATA 
TCTCGGCCAA AGAGTGCAGA CCCTCCTCGC TCTCCTCTGC TCAAACGAGT CCAGTCCGCA 
GAGAAACTCG CCGCCTCTCT TTCTTCTTCA TCCTCCTCTC CTTCTCCCTC TTCGGCTGCT 
GATAAAAAGC TTCCAGTTGG TGCCAGCCGC AAACACAGTC TTGATACCTC ACATTCAGAG 
TTTAAAAAAG AGATGCTGCA GCGAGACCCT AGTCTACAGA GCCTGCAAGA GTCAGCCAGT 
GAGACTCTTA TGGGAGGAAG AGTGTCTCCT GCAGAAAAGG GAAGCCTGCA GAAAAGTTCA 
GTGCGTAAAC TGGGCAGGCA AGAAGGTCCC GAGTCTGGAA CAGGAACTCT GGGACTTGTT 
CCTGGAAAGA GTAAATTGAA AGACAAGCTG TCTGCCATAC GTGCTGAACG CCGCGAATCT 
CTTCAAAAGC AGGATGCCAT CCATGAAGTG GATTCATCTG AAGATGAGAC AGACGAAGGG 
TCGGAAGACA GCCAAGATGG GCGCAGAACT TGCTATGCTC CTCCTAGCCA TGTCTTGAGG 
CCTACAACTG TAATGGCCTC TCCCCATACC ATCAGAATGG GGCCAGCGGC TCCACCAACA 
CTGGGACTTT TTCCTTCAAC AGTTGCACCG CCATGCTCAC CTCAGCTCAT CAGAGGACTG 
CAGATACAAA ACCAAAGTAC CACTCAATCT CAGACACAAA CTTCAACCTC ACAACCAGCG 
CAAGCTGCAG TTAAACCCCC AGAACAGAAA CCAATAACCT CTGTTAAAGA CTCAAGTTCA 
ACGATAATTG ATGATTCGAA AAAACAGTCA CAGGCACAAG ACATTCAGTC CAGACCTTCT 
CCACATCAAC CCAGTCCTCT GTCTAGTACC TTTTCCACCC AAGGCAGCGC CTTTACCTCT 
GTTAGTAAAG ACCCATTTGT CAAGCCCACC ACACCTCCAT TGGATCCCCG GAGGACCCCA 
AAAATGTCTG AGCCAAGGTC AAAAACCAGT GGTAGAACTG ATTCTAGCAC TACTTCTGGA 
GTAGCCAGGG ATACCCCAGT TGCGACTACC CCAACAGGAG GAATCACTAA GGAGATGAAG 
GAGGCTGACA ATCGCAGACA GGCTGCAGCT GCTGCTGCTG CCTCTGCCGC CGCTGCTACC 
ACCTCTGCAT CCACTGCCTC TGAAAGTGGA ATGGATAAAG TTGTGTCTCA GCTCGCCACT 
GTGGCCAAGA GTGTGCTTGG ACCTGTCAAG CTCAATATCC CAGGTACTAA AGAGACCATG 
GAACGAACAA AGGACCAGCG TACCCAGGCA GATGCCACCC ACCCAAAGAT AAAAGAATGT 
CGCCTGGAGC ACCCTGATTC TCCTGACCAA TTGAGTCCAT CTGCTGCTTC GGGCTCCCAG 
TCTCCACGAC TGACTGAATC TCCATCTAAA CAAACATCAT CCAAATCTGA GCCAGCCTCA 
ACTTCTCAGA GACCTTTAGA GGTCCCAGGG ACTTTCAAAA GACAAACTTC TCCAATCCCT 
CAAGCTAGGG AAGAGCATCA GGATAGGTCA GGAACTCTGC AAAGTGGCAC CACATCAAGG 
CCTAGGTCTG AAGCACAATC ACAGACCCAG CAGGCAAAGC CCAGTTCCAC TACGTCACGA 
GATAAATCCA ACAAGACAAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001341305.4
CDS	1077..6860
Translation	MKKTESKNKRKLCTSEQQDSSTEGKDEELPAPPPLLFRKLSNPDLSPAAGKTKLQRQLSQDDSRGRRSSMAGILTGKQLLPLSSSMHGGVSQLAWQQHTGEPNNLVRMRSQSFGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYAPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPCSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_001341269.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001341269.7

ORF Insert Sequence:

ATGAAGAAAA CCGAGAGCAA AAACAAAAGG AAACTCTGCA CATCTGAGCA GCAGGACAGC 
AGCACTGAAG GAAAAGACGA GGAGCTGCCG GCGCCGCCTC CCCTGCTGTT CAGGAAGCTG 
AGTAACCCAG ACCTGTCTCC TGCCGCAGGA AAAACCAAAC TGCAGAGGCA GCTCAGTCAG 
GATGACAGCA GAGGCCGACG CAGCAGCATG GCTGGCATCC TCACTGGAAA GCAGCTTCTG 
CCTCTGTCCA GCAGTATGCA TGGAGGAGTG AGTCAGCTGG CCTGGCAGCA GCACACAGGG 
GAGCCCAATA ACTTGGTCCG CATGAGGAGT CAGTCCTTTG GCCAGTCTGC CCCGTCTCTC 
ACTGCGGGAC TGAAGGAGCT GAGTCTTCCT AGAAGAGGCA GTTTCTGTCG GACGAGCAAC 
CGAAAAAGTT TGATAGTGAC ATCCAGCACG TCTCCAACGC TGCCTCGACC CCATTCACCA 
CTGCACGGGC ATCCAGGAAG CAGCCCATTG GACAGCCCTC GCAATTTCTC CCCCAACACT 
GCAGCCCACT TCTCTTTTGT ACCAGCTCGA AGGACTGACG GTCGTCGCTG GTCTCTCGCC 
TCTCTGCCGT CCTCTGGCTA TGGAACAAAC ACCCCCAGTT CGACAGTCTC GTCATCATGT 
TCATCTCAGG AGAAGCTGCA CCAGTTGCCC TTCCAGCCCA CTGCAGACGA GCTGCATTTC 
CTCACCAAAC ACTTCAGCTC GGAGAGCATC ACGGATGAGG ATGGGAGACG ATCCCCAGCC 
ATGCGTCCGC GCTCCCGCAG TCTCAGCCCT GGACGGTCAC CAATATCATT TGACCATGAG 
ATTGTCATGA TGAACCATGT CTACAAGGAG CGTTTTCCTA AGGCCACTGC TCAGATGGAG 
GTGCGTCTGG CCGATTTCAT ATCCTCCTCA GCTCCAGAAA AAGTCATGCC TTTAGCTGAT 
GGCGTCCTGA GCTTCATCCA CCACCAGGTT ATCGAACTGT CTCGAGATTG TCTGGACAAA 
TCTCGTGAGG GTCTCATCAC CTCCCGCTAC TTCTATGAGC TACAAGAGAA CCTTGAAAAG 
CTACACCAGG ATGCTCATGA GCGTTCAGAA AGTGGGGAGG TGACATTTGT CACCCAGCTA 
GTTAAAAAGC TGATGATAAT CATCGCTCGG CCTGCCCGGC TGCTAGAATG TTTGGAGTTT 
GACCCGGAGG AGTTCTACCA CCTGTTAGAG GCAGCTGAAG GTCATGCTAA AGAAGGTCAA 
GGCATCAAGT CTGACATCCC TCGATACATC ATCAGCCAGC TGGGCCTCAC CAGGGACCCC 
CTTGAGGAAA TGGCCCAGCT GAGCAGCTAT GACAGCGGTA ACCCTGAAAC ACCAGAGACA 
GAGACAGATT CCACAGATAG TCGAGGAGCA ACCGTACAGC CACTCCAACC TCAGCCTCAA 
CCGCAGCCCA AGACTAAAAC ACCTCGCGAG GAAGACTTCG AGACCATTAA ACTCATCAGT 
AATGGAGCCT ATGGGGCTGT GTTTCTGGTG AGGCATAAAG AGACCCGTCA GCGATTTGCC 
ATGAAGAAGA TCAACAAGCA GAACCTGATC CTAAGAAACC AGATCCAGCA GGCCTTTGTG 
GAAAGAGACA TCTTGACATT TGCAGAAAAC CCTTTTGTTG TCAGCATGTT CTGCTCCTTT 
GAGACAAGGA GACATCTTTG CATGGTTATG GAGTATGTGG AGGGTGGGGA TTGTGCCACT 
CTCCTGAAGC ACATTGGAGC TCTGCCTGTG GATATGGCAC GCATGTACTT TGCTGAAACC 
GTTCTTGCAT TGGAATACCT TCACAACTAT GGTATTGTGC ACAGAGACCT CAAACCTGAC 
AATCTCCTGA TTACCTCAAT GGGACACGTC AAACTGACTG ACTTTGGCCT GTCCAAGATT 
GGTTTAATGA GCTTAACAAC TAATCTGTAT GAGGGCCACA TTGAAAAAGA TACTCGAGAA 
TTCTTGGACA AACAGGTGTG TGGTACTCCT GAATACATTG CTCCTGAGGT TATTCTGCGA 
CAGGGTTATG GAAAACCAGT TGACTGGTGG GCCATGGGAG TCATCTTGTA TGAGTTTCTG 
GTGGGCTGTG CTCCTTTCTT TGGAGATACA CCTGAAGAAC TGTTTGGACA AGTCATCAGT 
GATGAGATAA TCTGGCCAGA AGGTGATGAA GCTTTGCCTC CGGATGCTCA AGATCTCATC 
TCCAAACTTC TTCGGCAAAA CCCTCTAGAG CGTCTTGGGA CAGGGAGTGC GTTTGAGGTA 
AAACAGCACC GCTTCTTTAC TGATCTGGAC TGGAACAGCC TCCTGAGGCA GAAGGCTGAG 
TTCATTCCTC AGCTGGAGTC GGAGGATGAC ACTAGCTACT TTGACACACG CTCAGATCGT 
TATCATCATG TGGACTCAGA GGAAGAAGAT GACACAAATG ATGACGACCA TTTGGAGATC 
CGTCAGTTCT CCTCCTGTTC GCCACGATTC AGCAAGGTGT ATAGTAGTAT GGAGCGTCTT 
TCCTTGCATG AGGAGAAACG CACCCCTCCT CCTACTAAGC GCAGTCTGAG TGAAGAGGGA 
GGAGAGCGGA TCGACAGTCT AAGTGGCCTA AAGTCCAGAG ACCGAAGCTG GTTGGTGGGC 
TCACCTGAAA TTCTCAGAAA GCGTCTCTCT GTTTCCGAGT CTTCTCACAC CGAGAGTGAC 
TCGAGTCCAC CTCTGACTGT TCGTCGCCGC TGCTGCTCGG CCATCATTGA AATGCCTCGC 
TTCGCCATAT CCAGTGAAGA GGATAGCTGT GTGGGGAGCA GGAAGACCCC TGTGAGAGGC 
CCTCGAGGGG ATGATCTGCC TCAGGCCATA CCAGAGCTCC CTGTAGAGAG AGAGCTCAGG 
CTGGATGAAT CGCCCACCAC GCCAGGATCC ACTTCCAGTC AAATCAGCCA GTCCACACTC 
ACACCTGGTA GTTCTGGTGA TGTGTTAGAT CGTGCTTCTC GCTATAGTGC TGAAGTTTCT 
GAAAACTCCA CTCCAAGAGC CATCAGTGAT TTGGCGGCTC GAAGAGCTCG CCACAGACTG 
CTTTCAGGAG ATACAGATAA ACACACATCA CGTCCACTTA GCAAAGTCAT CAAATCAGCT 
TCAGCAACAA CCCTGTCACT CATGATCCCT GCTGATCACC ATGGTGCTTC TCCACTGGCC 
AGTCCCATGT CGCCTCACTC TCTCTCTTCG AACCCCTCAT CACGTGACTC CTCGCCCAGC 
CGTGACCTTT CCCCAGCTGT CTGTAGTGTT AAACCTGCCA TAATCATCCA TCGAGCTGGA 
AAGAAATATG GTTTTACTTT ACGTGCAATC CGGGTTTACA TGGGAGACAC AGACATATAT 
ACTGTCCATC ACATGGTCTG GCATGTGGAG GATGGAGGAC CTGCTCATGA GGCTGGACTG 
AGAGAGGGAG ATCTCATCAC ACATGTTAAC GGAGAGCCTG TCCATGGACT TGTGCACACA 
GAAGTTGTTG AGCTCATTCT TAAGAGTGGC AGTAAGGTGT CCATCTCTGC TACACCCTTT 
GAAAATACAT CAATTAAGGT TGGTCCTGCT CGCAAAACTA GCTACAAGTC CAAAATGGCC 
CGTCGCAACA AAAGAACCAA AACTAGAGAA GGACAAGACA GTAAGAAGAG GAATTCCCTC 
TTCCGTAAGA TCACCAAACA GGCGACCCTT CTTCACACCA GTCGCAGCCT GTCCTCTCTG 
AATCGCTCGG TCTCTTCTGG TGAGAGCGTC CCAGGCTCTC CTACACACAT GTCACCTCGT 
TCACCCACAC AGGGCTGTCG TTCAACACCT GACTCCGCCC ACTCAGTTGG TGGAAACTCA 
TCCCAAAGTA GCTCGCCTAG CTCCAGTGTG CCAAACTCTC CTGCCAGCTC TGGCCAGATC 
AGACCCAGCT CCTTGCATGG TCTGGCCCCC AAGCTGCAGC GCCAGTACCG ATCCCCCCGC 
CGGAAGTCTG CAGGCAACAT CCCTTTATCC CCTCTGGCCC GTACACCATC GCCCACTCCA 
CAGAGCACTT CTCCCCAGCG TTCCCCTTCT CCCCTGCCAA GCCACGGGCT CATCACTTCT 
TCAATAGGCC AGTCTTTTCC TGTCAAACTG CACTCTTCAC CTCCATTAGT AAGACAGATA 
TCTCGGCCAA AGAGTGCAGA CCCTCCTCGC TCTCCTCTGC TCAAACGAGT CCAGTCCGCA 
GAGAAACTCG CCGCCTCTCT TTCTTCTTCA TCCTCCTCTC CTTCTCCCTC TTCGGCTGCT 
GATAAAAAGC TTCCAGTTGG TGCCAGCCGC AAACACAGTC TTGATACCTC ACATTCAGAG 
TTTAAAAAAG AGATGCTGCA GCGAGACCCT AGTCTACAGA GCCTGCAAGA GTCAGCCAGT 
GAGACTCTTA TGGGAGGAAG AGTGTCTCCT GCAGAAAAGG GAAGCCTGCA GAAAAGTTCA 
GTGCGTAAAC TGGGCAGGCA AGAAGGTCCC GAGTCTGGAA CAGGAACTCT GGGACTTGTT 
CCTGGAAAGA GTAAATTGAA AGACAAGCTG TCTGCCATAC GTGCTGAACG CCGCGAATCT 
CTTCAAAAGC AGGATGCCAT CCATGAAGTG GATTCATCTG AAGATGAGAC AGACGAAGGG 
TCGGAAGACA GCCAAGATGG GCGCAGAACT TGCTATGCTC CTCCTAGCCA TGTCTTGAGG 
CCTACAACTG TAATGGCCTC TCCCCATACC ATCAGAATGG GGCCAGCGGC TCCACCAACA 
CTGGGACTTT TTCCTTCAAC AGTTGCACCG CCATGCTCAC CTCAGCTCAT CAGAGGACTG 
CAGATACAAA ACCAAAGTAC CACTCAATCT CAGACACAAA CTTCAACCTC ACAACCAGCG 
CAAGCTGCAG TTAAACCCCC AGAACAGAAA CCAATAACCT CTGTTAAAGA CTCAAGTTCA 
ACGATAATTG ATGATTCGAA AAAACAGTCA CAGGCACAAG ACATTCAGTC CAGACCTTCT 
CCACATCAAC CCAGTCCTCT GTCTAGTACC TTTTCCACCC AAGGCAGCGC CTTTACCTCT 
GTTAGTAAAG ACCCATTTGT CAAGCCCACC ACACCTCCAT TGGATCCCCG GAGGACCCCA 
AAAATGTCTG AGCCAAGGTC AAAAACCAGT GGTAGAACTG ATTCTAGCAC TACTTCTGGA 
GTAGCCAGGG ATACCCCAGT TGCGACTACC CCAACAGGAG GAATCACTAA GGAGATGAAG 
GAGGCTGACA ATCGCAGACA GGCTGCAGCT GCTGCTGCTG CCTCTGCCGC CGCTGCTACC 
ACCTCTGCAT CCACTGCCTC TGAAAGTGGA ATGGATAAAG TTGTGTCTCA GCTCGCCACT 
GTGGCCAAGA GTGTGCTTGG ACCTGTCAAG CTCAATATCC CAGGTACTAA AGAGACCATG 
GAACGAACAA AGGACCAGCG TACCCAGGCA GATGCCACCC ACCCAAAGAT AAAAGAATGT 
CGCCTGGAGC ACCCTGATTC TCCTGACCAA TTGAGTCCAT CTGCTGCTTC GGGCTCCCAG 
TCTCCACGAC TGACTGAATC TCCATCTAAA CAAACATCAT CCAAATCTGA GCCAGCCTCA 
ACTTCTCAGA GACCTTTAGA GGTCCCAGGG ACTTTCAAAA GACAAACTTC TCCAATCCCT 
CAAGCTAGGG AAGAGCATCA GGATAGGTCA GGAACTCTGC AAAGTGGCAC CACATCAAGG 
CCTAGGTCTG AAGCACAATC ACAGACCCAG CAGGCAAAGC CCAGTTCCAC TACGTCACGA 
GATAAATCCA ACAAGACAAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_001341269.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001341305.4 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X5, mRNA.

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CloneID	ODa11957
Clone ID Related Accession (Same CDS sequence)	XM_001341269.7 , XM_001341269.8
Accession Version	XM_001341269.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001341269.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAA CCGAGAGCAA AAACAAAAGG AAACTCTGCA CATCTGAGCA GCAGGACAGC 
AGCACTGAAG GAAAAGACGA GGAGCTGCCG GCGCCGCCTC CCCTGCTGTT CAGGAAGCTG 
AGTAACCCAG ACCTGTCTCC TGCCGCAGGA AAAACCAAAC TGCAGAGGCA GCTCAGTCAG 
GATGACAGCA GAGGCCGACG CAGCAGCATG GCTGGCATCC TCACTGGAAA GCAGCTTCTG 
CCTCTGTCCA GCAGTATGCA TGGAGGAGTG AGTCAGCTGG CCTGGCAGCA GCACACAGGG 
GAGCCCAATA ACTTGGTCCG CATGAGGAGT CAGTCCTTTG GCCAGTCTGC CCCGTCTCTC 
ACTGCGGGAC TGAAGGAGCT GAGTCTTCCT AGAAGAGGCA GTTTCTGTCG GACGAGCAAC 
CGAAAAAGTT TGATAGTGAC ATCCAGCACG TCTCCAACGC TGCCTCGACC CCATTCACCA 
CTGCACGGGC ATCCAGGAAG CAGCCCATTG GACAGCCCTC GCAATTTCTC CCCCAACACT 
GCAGCCCACT TCTCTTTTGT ACCAGCTCGA AGGACTGACG GTCGTCGCTG GTCTCTCGCC 
TCTCTGCCGT CCTCTGGCTA TGGAACAAAC ACCCCCAGTT CGACAGTCTC GTCATCATGT 
TCATCTCAGG AGAAGCTGCA CCAGTTGCCC TTCCAGCCCA CTGCAGACGA GCTGCATTTC 
CTCACCAAAC ACTTCAGCTC GGAGAGCATC ACGGATGAGG ATGGGAGACG ATCCCCAGCC 
ATGCGTCCGC GCTCCCGCAG TCTCAGCCCT GGACGGTCAC CAATATCATT TGACCATGAG 
ATTGTCATGA TGAACCATGT CTACAAGGAG CGTTTTCCTA AGGCCACTGC TCAGATGGAG 
GTGCGTCTGG CCGATTTCAT ATCCTCCTCA GCTCCAGAAA AAGTCATGCC TTTAGCTGAT 
GGCGTCCTGA GCTTCATCCA CCACCAGGTT ATCGAACTGT CTCGAGATTG TCTGGACAAA 
TCTCGTGAGG GTCTCATCAC CTCCCGCTAC TTCTATGAGC TACAAGAGAA CCTTGAAAAG 
CTACACCAGG ATGCTCATGA GCGTTCAGAA AGTGGGGAGG TGACATTTGT CACCCAGCTA 
GTTAAAAAGC TGATGATAAT CATCGCTCGG CCTGCCCGGC TGCTAGAATG TTTGGAGTTT 
GACCCGGAGG AGTTCTACCA CCTGTTAGAG GCAGCTGAAG GTCATGCTAA AGAAGGTCAA 
GGCATCAAGT CTGACATCCC TCGATACATC ATCAGCCAGC TGGGCCTCAC CAGGGACCCC 
CTTGAGGAAA TGGCCCAGCT GAGCAGCTAT GACAGCGGTA ACCCTGAAAC ACCAGAGACA 
GAGACAGATT CCACAGATAG TCGAGGAGCA ACCGTACAGC CACTCCAACC TCAGCCTCAA 
CCGCAGCCCA AGACTAAAAC ACCTCGCGAG GAAGACTTCG AGACCATTAA ACTCATCAGT 
AATGGAGCCT ATGGGGCTGT GTTTCTGGTG AGGCATAAAG AGACCCGTCA GCGATTTGCC 
ATGAAGAAGA TCAACAAGCA GAACCTGATC CTAAGAAACC AGATCCAGCA GGCCTTTGTG 
GAAAGAGACA TCTTGACATT TGCAGAAAAC CCTTTTGTTG TCAGCATGTT CTGCTCCTTT 
GAGACAAGGA GACATCTTTG CATGGTTATG GAGTATGTGG AGGGTGGGGA TTGTGCCACT 
CTCCTGAAGC ACATTGGAGC TCTGCCTGTG GATATGGCAC GCATGTACTT TGCTGAAACC 
GTTCTTGCAT TGGAATACCT TCACAACTAT GGTATTGTGC ACAGAGACCT CAAACCTGAC 
AATCTCCTGA TTACCTCAAT GGGACACGTC AAACTGACTG ACTTTGGCCT GTCCAAGATT 
GGTTTAATGA GCTTAACAAC TAATCTGTAT GAGGGCCACA TTGAAAAAGA TACTCGAGAA 
TTCTTGGACA AACAGGTGTG TGGTACTCCT GAATACATTG CTCCTGAGGT TATTCTGCGA 
CAGGGTTATG GAAAACCAGT TGACTGGTGG GCCATGGGAG TCATCTTGTA TGAGTTTCTG 
GTGGGCTGTG CTCCTTTCTT TGGAGATACA CCTGAAGAAC TGTTTGGACA AGTCATCAGT 
GATGAGATAA TCTGGCCAGA AGGTGATGAA GCTTTGCCTC CGGATGCTCA AGATCTCATC 
TCCAAACTTC TTCGGCAAAA CCCTCTAGAG CGTCTTGGGA CAGGGAGTGC GTTTGAGGTA 
AAACAGCACC GCTTCTTTAC TGATCTGGAC TGGAACAGCC TCCTGAGGCA GAAGGCTGAG 
TTCATTCCTC AGCTGGAGTC GGAGGATGAC ACTAGCTACT TTGACACACG CTCAGATCGT 
TATCATCATG TGGACTCAGA GGAAGAAGAT GACACAAATG ATGACGACCA TTTGGAGATC 
CGTCAGTTCT CCTCCTGTTC GCCACGATTC AGCAAGGTGT ATAGTAGTAT GGAGCGTCTT 
TCCTTGCATG AGGAGAAACG CACCCCTCCT CCTACTAAGC GCAGTCTGAG TGAAGAGGGA 
GGAGAGCGGA TCGACAGTCT AAGTGGCCTA AAGTCCAGAG ACCGAAGCTG GTTGGTGGGC 
TCACCTGAAA TTCTCAGAAA GCGTCTCTCT GTTTCCGAGT CTTCTCACAC CGAGAGTGAC 
TCGAGTCCAC CTCTGACTGT TCGTCGCCGC TGCTGCTCGG CCATCATTGA AATGCCTCGC 
TTCGCCATAT CCAGTGAAGA GGATAGCTGT GTGGGGAGCA GGAAGACCCC TGTGAGAGGC 
CCTCGAGGGG ATGATCTGCC TCAGGCCATA CCAGAGCTCC CTGTAGAGAG AGAGCTCAGG 
CTGGATGAAT CGCCCACCAC GCCAGGATCC ACTTCCAGTC AAATCAGCCA GTCCACACTC 
ACACCTGGTA GTTCTGGTGA TGTGTTAGAT CGTGCTTCTC GCTATAGTGC TGAAGTTTCT 
GAAAACTCCA CTCCAAGAGC CATCAGTGAT TTGGCGGCTC GAAGAGCTCG CCACAGACTG 
CTTTCAGGAG ATACAGATAA ACACACATCA CGTCCACTTA GCAAAGTCAT CAAATCAGCT 
TCAGCAACAA CCCTGTCACT CATGATCCCT GCTGATCACC ATGGTGCTTC TCCACTGGCC 
AGTCCCATGT CGCCTCACTC TCTCTCTTCG AACCCCTCAT CACGTGACTC CTCGCCCAGC 
CGTGACCTTT CCCCAGCTGT CTGTAGTGTT AAACCTGCCA TAATCATCCA TCGAGCTGGA 
AAGAAATATG GTTTTACTTT ACGTGCAATC CGGGTTTACA TGGGAGACAC AGACATATAT 
ACTGTCCATC ACATGGTCTG GCATGTGGAG GATGGAGGAC CTGCTCATGA GGCTGGACTG 
AGAGAGGGAG ATCTCATCAC ACATGTTAAC GGAGAGCCTG TCCATGGACT TGTGCACACA 
GAAGTTGTTG AGCTCATTCT TAAGAGTGGC AGTAAGGTGT CCATCTCTGC TACACCCTTT 
GAAAATACAT CAATTAAGGT TGGTCCTGCT CGCAAAACTA GCTACAAGTC CAAAATGGCC 
CGTCGCAACA AAAGAACCAA AACTAGAGAA GGACAAGACA GTAAGAAGAG GAATTCCCTC 
TTCCGTAAGA TCACCAAACA GGCGACCCTT CTTCACACCA GTCGCAGCCT GTCCTCTCTG 
AATCGCTCGG TCTCTTCTGG TGAGAGCGTC CCAGGCTCTC CTACACACAT GTCACCTCGT 
TCACCCACAC AGGGCTGTCG TTCAACACCT GACTCCGCCC ACTCAGTTGG TGGAAACTCA 
TCCCAAAGTA GCTCGCCTAG CTCCAGTGTG CCAAACTCTC CTGCCAGCTC TGGCCAGATC 
AGACCCAGCT CCTTGCATGG TCTGGCCCCC AAGCTGCAGC GCCAGTACCG ATCCCCCCGC 
CGGAAGTCTG CAGGCAACAT CCCTTTATCC CCTCTGGCCC GTACACCATC GCCCACTCCA 
CAGAGCACTT CTCCCCAGCG TTCCCCTTCT CCCCTGCCAA GCCACGGGCT CATCACTTCT 
TCAATAGGCC AGTCTTTTCC TGTCAAACTG CACTCTTCAC CTCCATTAGT AAGACAGATA 
TCTCGGCCAA AGAGTGCAGA CCCTCCTCGC TCTCCTCTGC TCAAACGAGT CCAGTCCGCA 
GAGAAACTCG CCGCCTCTCT TTCTTCTTCA TCCTCCTCTC CTTCTCCCTC TTCGGCTGCT 
GATAAAAAGC TTCCAGTTGG TGCCAGCCGC AAACACAGTC TTGATACCTC ACATTCAGAG 
TTTAAAAAAG AGATGCTGCA GCGAGACCCT AGTCTACAGA GCCTGCAAGA GTCAGCCAGT 
GAGACTCTTA TGGGAGGAAG AGTGTCTCCT GCAGAAAAGG GAAGCCTGCA GAAAAGTTCA 
GTGCGTAAAC TGGGCAGGCA AGAAGGTCCC GAGTCTGGAA CAGGAACTCT GGGACTTGTT 
CCTGGAAAGA GTAAATTGAA AGACAAGCTG TCTGCCATAC GTGCTGAACG CCGCGAATCT 
CTTCAAAAGC AGGATGCCAT CCATGAAGTG GATTCATCTG AAGATGAGAC AGACGAAGGG 
TCGGAAGACA GCCAAGATGG GCGCAGAACT TGCTATGCTC CTCCTAGCCA TGTCTTGAGG 
CCTACAACTG TAATGGCCTC TCCCCATACC ATCAGAATGG GGCCAGCGGC TCCACCAACA 
CTGGGACTTT TTCCTTCAAC AGTTGCACCG CCATGCTCAC CTCAGCTCAT CAGAGGACTG 
CAGATACAAA ACCAAAGTAC CACTCAATCT CAGACACAAA CTTCAACCTC ACAACCAGCG 
CAAGCTGCAG TTAAACCCCC AGAACAGAAA CCAATAACCT CTGTTAAAGA CTCAAGTTCA 
ACGATAATTG ATGATTCGAA AAAACAGTCA CAGGCACAAG ACATTCAGTC CAGACCTTCT 
CCACATCAAC CCAGTCCTCT GTCTAGTACC TTTTCCACCC AAGGCAGCGC CTTTACCTCT 
GTTAGTAAAG ACCCATTTGT CAAGCCCACC ACACCTCCAT TGGATCCCCG GAGGACCCCA 
AAAATGTCTG AGCCAAGGTC AAAAACCAGT GGTAGAACTG ATTCTAGCAC TACTTCTGGA 
GTAGCCAGGG ATACCCCAGT TGCGACTACC CCAACAGGAG GAATCACTAA GGAGATGAAG 
GAGGCTGACA ATCGCAGACA GGCTGCAGCT GCTGCTGCTG CCTCTGCCGC CGCTGCTACC 
ACCTCTGCAT CCACTGCCTC TGAAAGTGGA ATGGATAAAG TTGTGTCTCA GCTCGCCACT 
GTGGCCAAGA GTGTGCTTGG ACCTGTCAAG CTCAATATCC CAGGTACTAA AGAGACCATG 
GAACGAACAA AGGACCAGCG TACCCAGGCA GATGCCACCC ACCCAAAGAT AAAAGAATGT 
CGCCTGGAGC ACCCTGATTC TCCTGACCAA TTGAGTCCAT CTGCTGCTTC GGGCTCCCAG 
TCTCCACGAC TGACTGAATC TCCATCTAAA CAAACATCAT CCAAATCTGA GCCAGCCTCA 
ACTTCTCAGA GACCTTTAGA GGTCCCAGGG ACTTTCAAAA GACAAACTTC TCCAATCCCT 
CAAGCTAGGG AAGAGCATCA GGATAGGTCA GGAACTCTGC AAAGTGGCAC CACATCAAGG 
CCTAGGTCTG AAGCACAATC ACAGACCCAG CAGGCAAAGC CCAGTTCCAC TACGTCACGA 
GATAAATCCA ACAAGACAAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001341305.4
CDS	1056..6839
Translation	MKKTESKNKRKLCTSEQQDSSTEGKDEELPAPPPLLFRKLSNPDLSPAAGKTKLQRQLSQDDSRGRRSSMAGILTGKQLLPLSSSMHGGVSQLAWQQHTGEPNNLVRMRSQSFGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYAPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPCSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_001341269.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001341269.8

ORF Insert Sequence:

ATGAAGAAAA CCGAGAGCAA AAACAAAAGG AAACTCTGCA CATCTGAGCA GCAGGACAGC 
AGCACTGAAG GAAAAGACGA GGAGCTGCCG GCGCCGCCTC CCCTGCTGTT CAGGAAGCTG 
AGTAACCCAG ACCTGTCTCC TGCCGCAGGA AAAACCAAAC TGCAGAGGCA GCTCAGTCAG 
GATGACAGCA GAGGCCGACG CAGCAGCATG GCTGGCATCC TCACTGGAAA GCAGCTTCTG 
CCTCTGTCCA GCAGTATGCA TGGAGGAGTG AGTCAGCTGG CCTGGCAGCA GCACACAGGG 
GAGCCCAATA ACTTGGTCCG CATGAGGAGT CAGTCCTTTG GCCAGTCTGC CCCGTCTCTC 
ACTGCGGGAC TGAAGGAGCT GAGTCTTCCT AGAAGAGGCA GTTTCTGTCG GACGAGCAAC 
CGAAAAAGTT TGATAGTGAC ATCCAGCACG TCTCCAACGC TGCCTCGACC CCATTCACCA 
CTGCACGGGC ATCCAGGAAG CAGCCCATTG GACAGCCCTC GCAATTTCTC CCCCAACACT 
GCAGCCCACT TCTCTTTTGT ACCAGCTCGA AGGACTGACG GTCGTCGCTG GTCTCTCGCC 
TCTCTGCCGT CCTCTGGCTA TGGAACAAAC ACCCCCAGTT CGACAGTCTC GTCATCATGT 
TCATCTCAGG AGAAGCTGCA CCAGTTGCCC TTCCAGCCCA CTGCAGACGA GCTGCATTTC 
CTCACCAAAC ACTTCAGCTC GGAGAGCATC ACGGATGAGG ATGGGAGACG ATCCCCAGCC 
ATGCGTCCGC GCTCCCGCAG TCTCAGCCCT GGACGGTCAC CAATATCATT TGACCATGAG 
ATTGTCATGA TGAACCATGT CTACAAGGAG CGTTTTCCTA AGGCCACTGC TCAGATGGAG 
GTGCGTCTGG CCGATTTCAT ATCCTCCTCA GCTCCAGAAA AAGTCATGCC TTTAGCTGAT 
GGCGTCCTGA GCTTCATCCA CCACCAGGTT ATCGAACTGT CTCGAGATTG TCTGGACAAA 
TCTCGTGAGG GTCTCATCAC CTCCCGCTAC TTCTATGAGC TACAAGAGAA CCTTGAAAAG 
CTACACCAGG ATGCTCATGA GCGTTCAGAA AGTGGGGAGG TGACATTTGT CACCCAGCTA 
GTTAAAAAGC TGATGATAAT CATCGCTCGG CCTGCCCGGC TGCTAGAATG TTTGGAGTTT 
GACCCGGAGG AGTTCTACCA CCTGTTAGAG GCAGCTGAAG GTCATGCTAA AGAAGGTCAA 
GGCATCAAGT CTGACATCCC TCGATACATC ATCAGCCAGC TGGGCCTCAC CAGGGACCCC 
CTTGAGGAAA TGGCCCAGCT GAGCAGCTAT GACAGCGGTA ACCCTGAAAC ACCAGAGACA 
GAGACAGATT CCACAGATAG TCGAGGAGCA ACCGTACAGC CACTCCAACC TCAGCCTCAA 
CCGCAGCCCA AGACTAAAAC ACCTCGCGAG GAAGACTTCG AGACCATTAA ACTCATCAGT 
AATGGAGCCT ATGGGGCTGT GTTTCTGGTG AGGCATAAAG AGACCCGTCA GCGATTTGCC 
ATGAAGAAGA TCAACAAGCA GAACCTGATC CTAAGAAACC AGATCCAGCA GGCCTTTGTG 
GAAAGAGACA TCTTGACATT TGCAGAAAAC CCTTTTGTTG TCAGCATGTT CTGCTCCTTT 
GAGACAAGGA GACATCTTTG CATGGTTATG GAGTATGTGG AGGGTGGGGA TTGTGCCACT 
CTCCTGAAGC ACATTGGAGC TCTGCCTGTG GATATGGCAC GCATGTACTT TGCTGAAACC 
GTTCTTGCAT TGGAATACCT TCACAACTAT GGTATTGTGC ACAGAGACCT CAAACCTGAC 
AATCTCCTGA TTACCTCAAT GGGACACGTC AAACTGACTG ACTTTGGCCT GTCCAAGATT 
GGTTTAATGA GCTTAACAAC TAATCTGTAT GAGGGCCACA TTGAAAAAGA TACTCGAGAA 
TTCTTGGACA AACAGGTGTG TGGTACTCCT GAATACATTG CTCCTGAGGT TATTCTGCGA 
CAGGGTTATG GAAAACCAGT TGACTGGTGG GCCATGGGAG TCATCTTGTA TGAGTTTCTG 
GTGGGCTGTG CTCCTTTCTT TGGAGATACA CCTGAAGAAC TGTTTGGACA AGTCATCAGT 
GATGAGATAA TCTGGCCAGA AGGTGATGAA GCTTTGCCTC CGGATGCTCA AGATCTCATC 
TCCAAACTTC TTCGGCAAAA CCCTCTAGAG CGTCTTGGGA CAGGGAGTGC GTTTGAGGTA 
AAACAGCACC GCTTCTTTAC TGATCTGGAC TGGAACAGCC TCCTGAGGCA GAAGGCTGAG 
TTCATTCCTC AGCTGGAGTC GGAGGATGAC ACTAGCTACT TTGACACACG CTCAGATCGT 
TATCATCATG TGGACTCAGA GGAAGAAGAT GACACAAATG ATGACGACCA TTTGGAGATC 
CGTCAGTTCT CCTCCTGTTC GCCACGATTC AGCAAGGTGT ATAGTAGTAT GGAGCGTCTT 
TCCTTGCATG AGGAGAAACG CACCCCTCCT CCTACTAAGC GCAGTCTGAG TGAAGAGGGA 
GGAGAGCGGA TCGACAGTCT AAGTGGCCTA AAGTCCAGAG ACCGAAGCTG GTTGGTGGGC 
TCACCTGAAA TTCTCAGAAA GCGTCTCTCT GTTTCCGAGT CTTCTCACAC CGAGAGTGAC 
TCGAGTCCAC CTCTGACTGT TCGTCGCCGC TGCTGCTCGG CCATCATTGA AATGCCTCGC 
TTCGCCATAT CCAGTGAAGA GGATAGCTGT GTGGGGAGCA GGAAGACCCC TGTGAGAGGC 
CCTCGAGGGG ATGATCTGCC TCAGGCCATA CCAGAGCTCC CTGTAGAGAG AGAGCTCAGG 
CTGGATGAAT CGCCCACCAC GCCAGGATCC ACTTCCAGTC AAATCAGCCA GTCCACACTC 
ACACCTGGTA GTTCTGGTGA TGTGTTAGAT CGTGCTTCTC GCTATAGTGC TGAAGTTTCT 
GAAAACTCCA CTCCAAGAGC CATCAGTGAT TTGGCGGCTC GAAGAGCTCG CCACAGACTG 
CTTTCAGGAG ATACAGATAA ACACACATCA CGTCCACTTA GCAAAGTCAT CAAATCAGCT 
TCAGCAACAA CCCTGTCACT CATGATCCCT GCTGATCACC ATGGTGCTTC TCCACTGGCC 
AGTCCCATGT CGCCTCACTC TCTCTCTTCG AACCCCTCAT CACGTGACTC CTCGCCCAGC 
CGTGACCTTT CCCCAGCTGT CTGTAGTGTT AAACCTGCCA TAATCATCCA TCGAGCTGGA 
AAGAAATATG GTTTTACTTT ACGTGCAATC CGGGTTTACA TGGGAGACAC AGACATATAT 
ACTGTCCATC ACATGGTCTG GCATGTGGAG GATGGAGGAC CTGCTCATGA GGCTGGACTG 
AGAGAGGGAG ATCTCATCAC ACATGTTAAC GGAGAGCCTG TCCATGGACT TGTGCACACA 
GAAGTTGTTG AGCTCATTCT TAAGAGTGGC AGTAAGGTGT CCATCTCTGC TACACCCTTT 
GAAAATACAT CAATTAAGGT TGGTCCTGCT CGCAAAACTA GCTACAAGTC CAAAATGGCC 
CGTCGCAACA AAAGAACCAA AACTAGAGAA GGACAAGACA GTAAGAAGAG GAATTCCCTC 
TTCCGTAAGA TCACCAAACA GGCGACCCTT CTTCACACCA GTCGCAGCCT GTCCTCTCTG 
AATCGCTCGG TCTCTTCTGG TGAGAGCGTC CCAGGCTCTC CTACACACAT GTCACCTCGT 
TCACCCACAC AGGGCTGTCG TTCAACACCT GACTCCGCCC ACTCAGTTGG TGGAAACTCA 
TCCCAAAGTA GCTCGCCTAG CTCCAGTGTG CCAAACTCTC CTGCCAGCTC TGGCCAGATC 
AGACCCAGCT CCTTGCATGG TCTGGCCCCC AAGCTGCAGC GCCAGTACCG ATCCCCCCGC 
CGGAAGTCTG CAGGCAACAT CCCTTTATCC CCTCTGGCCC GTACACCATC GCCCACTCCA 
CAGAGCACTT CTCCCCAGCG TTCCCCTTCT CCCCTGCCAA GCCACGGGCT CATCACTTCT 
TCAATAGGCC AGTCTTTTCC TGTCAAACTG CACTCTTCAC CTCCATTAGT AAGACAGATA 
TCTCGGCCAA AGAGTGCAGA CCCTCCTCGC TCTCCTCTGC TCAAACGAGT CCAGTCCGCA 
GAGAAACTCG CCGCCTCTCT TTCTTCTTCA TCCTCCTCTC CTTCTCCCTC TTCGGCTGCT 
GATAAAAAGC TTCCAGTTGG TGCCAGCCGC AAACACAGTC TTGATACCTC ACATTCAGAG 
TTTAAAAAAG AGATGCTGCA GCGAGACCCT AGTCTACAGA GCCTGCAAGA GTCAGCCAGT 
GAGACTCTTA TGGGAGGAAG AGTGTCTCCT GCAGAAAAGG GAAGCCTGCA GAAAAGTTCA 
GTGCGTAAAC TGGGCAGGCA AGAAGGTCCC GAGTCTGGAA CAGGAACTCT GGGACTTGTT 
CCTGGAAAGA GTAAATTGAA AGACAAGCTG TCTGCCATAC GTGCTGAACG CCGCGAATCT 
CTTCAAAAGC AGGATGCCAT CCATGAAGTG GATTCATCTG AAGATGAGAC AGACGAAGGG 
TCGGAAGACA GCCAAGATGG GCGCAGAACT TGCTATGCTC CTCCTAGCCA TGTCTTGAGG 
CCTACAACTG TAATGGCCTC TCCCCATACC ATCAGAATGG GGCCAGCGGC TCCACCAACA 
CTGGGACTTT TTCCTTCAAC AGTTGCACCG CCATGCTCAC CTCAGCTCAT CAGAGGACTG 
CAGATACAAA ACCAAAGTAC CACTCAATCT CAGACACAAA CTTCAACCTC ACAACCAGCG 
CAAGCTGCAG TTAAACCCCC AGAACAGAAA CCAATAACCT CTGTTAAAGA CTCAAGTTCA 
ACGATAATTG ATGATTCGAA AAAACAGTCA CAGGCACAAG ACATTCAGTC CAGACCTTCT 
CCACATCAAC CCAGTCCTCT GTCTAGTACC TTTTCCACCC AAGGCAGCGC CTTTACCTCT 
GTTAGTAAAG ACCCATTTGT CAAGCCCACC ACACCTCCAT TGGATCCCCG GAGGACCCCA 
AAAATGTCTG AGCCAAGGTC AAAAACCAGT GGTAGAACTG ATTCTAGCAC TACTTCTGGA 
GTAGCCAGGG ATACCCCAGT TGCGACTACC CCAACAGGAG GAATCACTAA GGAGATGAAG 
GAGGCTGACA ATCGCAGACA GGCTGCAGCT GCTGCTGCTG CCTCTGCCGC CGCTGCTACC 
ACCTCTGCAT CCACTGCCTC TGAAAGTGGA ATGGATAAAG TTGTGTCTCA GCTCGCCACT 
GTGGCCAAGA GTGTGCTTGG ACCTGTCAAG CTCAATATCC CAGGTACTAA AGAGACCATG 
GAACGAACAA AGGACCAGCG TACCCAGGCA GATGCCACCC ACCCAAAGAT AAAAGAATGT 
CGCCTGGAGC ACCCTGATTC TCCTGACCAA TTGAGTCCAT CTGCTGCTTC GGGCTCCCAG 
TCTCCACGAC TGACTGAATC TCCATCTAAA CAAACATCAT CCAAATCTGA GCCAGCCTCA 
ACTTCTCAGA GACCTTTAGA GGTCCCAGGG ACTTTCAAAA GACAAACTTC TCCAATCCCT 
CAAGCTAGGG AAGAGCATCA GGATAGGTCA GGAACTCTGC AAAGTGGCAC CACATCAAGG 
CCTAGGTCTG AAGCACAATC ACAGACCCAG CAGGCAAAGC CCAGTTCCAC TACGTCACGA 
GATAAATCCA ACAAGACAAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_005166291.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166348.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X1, mRNA.

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CloneID	ODa11958
Clone ID Related Accession (Same CDS sequence)	XM_005166291.4 , XM_005166291.3
Accession Version	XM_005166291.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166291.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACT CCTTCTGGAC CGTTCTCTCC AATTTCTCCC TGCCGGAGAG AAGCATGCTC 
CGCCGATCCA AGAGCTGTCG GACGAGCAAC CGAAAAAGTT TGATAGTGAC ATCCAGCACG 
TCTCCAACGC TGCCTCGACC CCATTCACCA CTGCACGGGC ATCCAGGAAG CAGCCCATTG 
GACAGCCCTC GCAATTTCTC CCCCAACACT GCAGCCCACT TCTCTTTTGT ACCAGCTCGA 
AGCCATGGAC ACAGGGCTGA CAGGACTGAC GGTCGTCGCT GGTCTCTCGC CTCTCTGCCG 
TCCTCTGGCT ATGGAACAAA CACCCCCAGT TCGACAGTCT CGTCATCATG TTCATCTCAG 
GAGAAGCTGC ACCAGTTGCC CTTCCAGCCC ACTGCAGACG AGCTGCATTT CCTCACCAAA 
CACTTCAGCT CGGAGAGCAT CACGGATGAG GATGGGAGAC GATCCCCAGC CATGCGTCCG 
CGCTCCCGCA GTCTCAGCCC TGGACGGTCA CCAATATCAT TTGACCATGA GATTGTCATG 
ATGAACCATG TCTACAAGGA GCGTTTTCCT AAGGCCACTG CTCAGATGGA GGTGCGTCTG 
GCCGATTTCA TATCCTCCTC AGCTCCAGAA AAAGTCATGC CTTTAGCTGA TGGCGTCCTG 
AGCTTCATCC ACCACCAGGT TATCGAACTG TCTCGAGATT GTCTGGACAA ATCTCGTGAG 
GGTCTCATCA CCTCCCGCTA CTTCTATGAG CTACAAGAGA ACCTTGAAAA GCTACACCAG 
GATGCTCATG AGCGTTCAGA AAGTGGGGAG GTGACATTTG TCACCCAGCT AGTTAAAAAG 
CTGATGATAA TCATCGCTCG GCCTGCCCGG CTGCTAGAAT GTTTGGAGTT TGACCCGGAG 
GAGTTCTACC ACCTGTTAGA GGCAGCTGAA GGTCATGCTA AAGAAGGTCA AGGCATCAAG 
TCTGACATCC CTCGATACAT CATCAGCCAG CTGGGCCTCA CCAGGGACCC CCTTGAGGAA 
ATGGCCCAGC TGAGCAGCTA TGACAGCGGT AACCCTGAAA CACCAGAGAC AGAGACAGAT 
TCCACAGATA GTCGAGGAGC AACCGTACAG CCACTCCAAC CTCAGCCTCA ACCGCAGCCC 
AAGACTAAAA CACCTCGCGA GGAAGACTTC GAGACCATTA AACTCATCAG TAATGGAGCC 
TATGGGGCTG TGTTTCTGGT GAGGCATAAA GAGACCCGTC AGCGATTTGC CATGAAGAAG 
ATCAACAAGC AGAACCTGAT CCTAAGAAAC CAGATCCAGC AGGCCTTTGT GGAAAGAGAC 
ATCTTGACAT TTGCAGAAAA CCCTTTTGTT GTCAGCATGT TCTGCTCCTT TGAGACAAGG 
AGACATCTTT GCATGGTTAT GGAGTATGTG GAGGGTGGGG ATTGTGCCAC TCTCCTGAAG 
CACATTGGAG CTCTGCCTGT GGATATGGCA CGCATGTACT TTGCTGAAAC CGTTCTTGCA 
TTGGAATACC TTCACAACTA TGGTATTGTG CACAGAGACC TCAAACCTGA CAATCTCCTG 
ATTACCTCAA TGGGACACGT CAAACTGACT GACTTTGGCC TGTCCAAGAT TGGTTTAATG 
AGCTTAACAA CTAATCTGTA TGAGGGCCAC ATTGAAAAAG ATACTCGAGA ATTCTTGGAC 
AAACAGGTGT GTGGTACTCC TGAATACATT GCTCCTGAGG TTATTCTGCG ACAGGGTTAT 
GGAAAACCAG TTGACTGGTG GGCCATGGGA GTCATCTTGT ATGAGTTTCT GGTGGGCTGT 
GCTCCTTTCT TTGGAGATAC ACCTGAAGAA CTGTTTGGAC AAGTCATCAG TGATGAGATA 
ATCTGGCCAG AAGGTGATGA AGCTTTGCCT CCGGATGCTC AAGATCTCAT CTCCAAACTT 
CTTCGGCAAA ACCCTCTAGA GCGTCTTGGG ACAGGGAGTG CGTTTGAGGT AAAACAGCAC 
CGCTTCTTTA CTGATCTGGA CTGGAACAGC CTCCTGAGGC AGAAGGCTGA GTTCATTCCT 
CAGCTGGAGT CGGAGGATGA CACTAGCTAC TTTGACACAC GCTCAGATCG TTATCATCAT 
GTGGACTCAG AGGAAGAAGA TGACACAAAT GATGACGACC ATTTGGAGAT CCGTCAGTTC 
TCCTCCTGTT CGCCACGATT CAGCAAGGTG TATAGTAGTA TGGAGCGTCT TTCCTTGCAT 
GAGGAGAAAC GCACCCCTCC TCCTACTAAG CGCAGTCTGA GTGAAGAGGG AGGAGAGCGG 
ATCGACAGTC TAAGTGGCCT AAAGTCCAGA GACCGAAGCT GGTTGGTGGG CTCACCTGAA 
ATTCTCAGAA AGCGTCTCTC TGTTTCCGAG TCTTCTCACA CCGAGAGTGA CTCGAGTCCA 
CCTCTGACTG TTCGTCGCCG CTGCTGCTCG GCCATCATTG AAATGCCTCG CTTCGCCATA 
TCCAGTGAAG AGGATAGCTG TGTGGGGAGC AGGAAGACCC CTGTGAGAGG CCCTCGAGGG 
GATGATCTGC CTCAGGCCAT ACCAGAGCTC CCTGTAGAGA GAGAGCTCAG GCTGGATGAA 
TCGCCCACCA CGCCAGGATC CACTTCCAGT CAAATCAGCC AGTCCACACT CACACCTGGT 
AGTTCTGGTG ATGTGTTAGA TCGTGCTTCT CGCTATAGTG CTGAAGTTTC TGAAAACTCC 
ACTCCAAGAG CCATCAGTGA TTTGGCGGCT CGAAGAGCTC GCCACAGACT GCTTTCAGGA 
GATACAGATA AACACACATC ACGTCCACTT AGCAAAGTCA TCAAATCAGC TTCAGCAACA 
ACCCTGTCAC TCATGATCCC TGCTGATCAC CATGGTGCTT CTCCACTGGC CAGTCCCATG 
TCGCCTCACT CTCTCTCTTC GAACCCCTCA TCACGTGACT CCTCGCCCAG CCGTGACCTT 
TCCCCAGCTG TCTGTAGTGT TAAACCTGCC ATAATCATCC ATCGAGCTGG AAAGAAATAT 
GGTTTTACTT TACGTGCAAT CCGGGTTTAC ATGGGAGACA CAGACATATA TACTGTCCAT 
CACATGGTCT GGCATGTGGA GGATGGAGGA CCTGCTCATG AGGCTGGACT GAGAGAGGGA 
GATCTCATCA CACATGTTAA CGGAGAGCCT GTCCATGGAC TTGTGCACAC AGAAGTTGTT 
GAGCTCATTC TTAAGAGTGG CAGTAAGGTG TCCATCTCTG CTACACCCTT TGAAAATACA 
TCAATTAAGG TTGGTCCTGC TCGCAAAACT AGCTACAAGT CCAAAATGGC CCGTCGCAAC 
AAAAGAACCA AAACTAGAGA AGGACAAGAC AGTAAGAAGA GGAATTCCCT CTTCCGTAAG 
ATCACCAAAC AGGCGACCCT TCTTCACACC AGTCGCAGCC TGTCCTCTCT GAATCGCTCG 
GTCTCTTCTG GTGAGAGCGT CCCAGGCTCT CCTACACACA TGTCACCTCG TTCACCCACA 
CAGGGCTGTC GTTCAACACC TGACTCCGCC CACTCAGTTG GTGGAAACTC ATCCCAAAGT 
AGCTCGCCTA GCTCCAGTGT GCCAAACTCT CCTGCCAGCT CTGGCCAGAT CAGACCCAGC 
TCCTTGCATG GTCTGGCCCC CAAGCTGCAG CGCCAGTACC GATCCCCCCG CCGGAAGTCT 
GCAGGCAACA TCCCTTTATC CCCTCTGGCC CGTACACCAT CGCCCACTCC ACAGAGCACT 
TCTCCCCAGC GTTCCCCTTC TCCCCTGCCA AGCCACGGGC TCATCACTTC TTCAATAGGC 
CAGTCTTTTC CTGTCAAACT GCACTCTTCA CCTCCATTAG TAAGACAGAT ATCTCGGCCA 
AAGAGTGCAG ACCCTCCTCG CTCTCCTCTG CTCAAACGAG TCCAGTCCGC AGAGAAACTC 
GCCGCCTCTC TTTCTTCTTC ATCCTCCTCT CCTTCTCCCT CTTCGGCTGC TGATAAAAAG 
CTTCCAGTTG GTGCCAGCCG CAAACACAGT CTTGATACCT CACATTCAGA GTTTAAAAAA 
GAGATGCTGC AGCGAGACCC TAGTCTACAG AGCCTGCAAG AGTCAGCCAG TGAGACTCTT 
ATGGGAGGAA GAGTGTCTCC TGCAGAAAAG GGAAGCCTGC AGAAAAGTTC AGTGCGTAAA 
CTGGGCAGGC AAGAAGGTCC CGAGTCTGGA ACAGGAACTC TGGGACTTGT TCCTGGAAAG 
AGTAAATTGA AAGACAAGCT GTCTGCCATA CGTGCTGAAC GCCGCGAATC TCTTCAAAAG 
CAGGATGCCA TCCATGAAGT GGATTCATCT GAAGATGAGA CAGACGAAGG GTCGGAAGAC 
AGCCAAGATG GGCGCAGAAC TTGCTATGCT CCTCCTAGCC ATGTCTTGAG GCCTACAACT 
GTAATGGCCT CTCCCCATAC CATCAGAATG GGGCCAGCGG CTCCACCAAC ACTGGGACTT 
TTTCCTTCAA CAGTTGCACC GCCATGCTCA CCTCAGCTCA TCAGAGGACT GCAGATACAA 
AACCAAAGTA CCACTCAATC TCAGACACAA ACTTCAACCT CACAACCAGC GCAAGCTGCA 
GTTAAACCCC CAGAACAGAA ACCAATAACC TCTGTTAAAG ACTCAAGTTC AACGATAATT 
GATGATTCGA AAAAACAGTC ACAGGCACAA GACATTCAGT CCAGACCTTC TCCACATCAA 
CCCAGTCCTC TGTCTAGTAC CTTTTCCACC CAAGGCAGCG CCTTTACCTC TGTTAGTAAA 
GACCCATTTG TCAAGCCCAC CACACCTCCA TTGGATCCCC GGAGGACCCC AAAAATGTCT 
GAGCCAAGGT CAAAAACCAG TGGTAGAACT GATTCTAGCA CTACTTCTGG AGTAGCCAGG 
GATACCCCAG TTGCGACTAC CCCAACAGGA GGAATCACTA AGGAGATGAA GGAGGCTGAC 
AATCGCAGAC AGGCTGCAGC TGCTGCTGCT GCCTCTGCCG CCGCTGCTAC CACCTCTGCA 
TCCACTGCCT CTGAAAGTGG AATGGATAAA GTTGTGTCTC AGCTCGCCAC TGTGGCCAAG 
AGTGTGCTTG GACCTGTCAA GCTCAATATC CCAGGTACTA AAGAGACCAT GGAACGAACA 
AAGGACCAGC GTACCCAGGC AGATGCCACC CACCCAAAGA TAAAAGAATG TCGCCTGGAG 
CACCCTGATT CTCCTGACCA ATTGAGTCCA TCTGCTGCTT CGGGCTCCCA GTCTCCACGA 
CTGACTGAAT CTCCATCTAA ACAAACATCA TCCAAATCTG AGCCAGCCTC AACTTCTCAG 
AGACCTTTAG AGGTCCCAGG GACTTTCAAA AGACAAACTT CTCCAATCCC TCAAGCTAGG 
GAAGAGCATC AGGATAGGTC AGGAACTCTG CAAAGTGGCA CCACATCAAG GCCTAGGTCT 
GAAGCACAAT CACAGACCCA GCAGGCAAAG CCCAGTTCCA CTACGTCACG AGATAAATCC 
AACAAGACAA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166348.1
CDS	133..5607
Translation	MSDSFWTVLSNFSLPERSMLRRSKSCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYAPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPCSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_005166291.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166291.4

ORF Insert Sequence:

ATGTCTGACT CCTTCTGGAC CGTTCTCTCC AATTTCTCCC TGCCGGAGAG AAGCATGCTC 
CGCCGATCCA AGAGCTGTCG GACGAGCAAC CGAAAAAGTT TGATAGTGAC ATCCAGCACG 
TCTCCAACGC TGCCTCGACC CCATTCACCA CTGCACGGGC ATCCAGGAAG CAGCCCATTG 
GACAGCCCTC GCAATTTCTC CCCCAACACT GCAGCCCACT TCTCTTTTGT ACCAGCTCGA 
AGCCATGGAC ACAGGGCTGA CAGGACTGAC GGTCGTCGCT GGTCTCTCGC CTCTCTGCCG 
TCCTCTGGCT ATGGAACAAA CACCCCCAGT TCGACAGTCT CGTCATCATG TTCATCTCAG 
GAGAAGCTGC ACCAGTTGCC CTTCCAGCCC ACTGCAGACG AGCTGCATTT CCTCACCAAA 
CACTTCAGCT CGGAGAGCAT CACGGATGAG GATGGGAGAC GATCCCCAGC CATGCGTCCG 
CGCTCCCGCA GTCTCAGCCC TGGACGGTCA CCAATATCAT TTGACCATGA GATTGTCATG 
ATGAACCATG TCTACAAGGA GCGTTTTCCT AAGGCCACTG CTCAGATGGA GGTGCGTCTG 
GCCGATTTCA TATCCTCCTC AGCTCCAGAA AAAGTCATGC CTTTAGCTGA TGGCGTCCTG 
AGCTTCATCC ACCACCAGGT TATCGAACTG TCTCGAGATT GTCTGGACAA ATCTCGTGAG 
GGTCTCATCA CCTCCCGCTA CTTCTATGAG CTACAAGAGA ACCTTGAAAA GCTACACCAG 
GATGCTCATG AGCGTTCAGA AAGTGGGGAG GTGACATTTG TCACCCAGCT AGTTAAAAAG 
CTGATGATAA TCATCGCTCG GCCTGCCCGG CTGCTAGAAT GTTTGGAGTT TGACCCGGAG 
GAGTTCTACC ACCTGTTAGA GGCAGCTGAA GGTCATGCTA AAGAAGGTCA AGGCATCAAG 
TCTGACATCC CTCGATACAT CATCAGCCAG CTGGGCCTCA CCAGGGACCC CCTTGAGGAA 
ATGGCCCAGC TGAGCAGCTA TGACAGCGGT AACCCTGAAA CACCAGAGAC AGAGACAGAT 
TCCACAGATA GTCGAGGAGC AACCGTACAG CCACTCCAAC CTCAGCCTCA ACCGCAGCCC 
AAGACTAAAA CACCTCGCGA GGAAGACTTC GAGACCATTA AACTCATCAG TAATGGAGCC 
TATGGGGCTG TGTTTCTGGT GAGGCATAAA GAGACCCGTC AGCGATTTGC CATGAAGAAG 
ATCAACAAGC AGAACCTGAT CCTAAGAAAC CAGATCCAGC AGGCCTTTGT GGAAAGAGAC 
ATCTTGACAT TTGCAGAAAA CCCTTTTGTT GTCAGCATGT TCTGCTCCTT TGAGACAAGG 
AGACATCTTT GCATGGTTAT GGAGTATGTG GAGGGTGGGG ATTGTGCCAC TCTCCTGAAG 
CACATTGGAG CTCTGCCTGT GGATATGGCA CGCATGTACT TTGCTGAAAC CGTTCTTGCA 
TTGGAATACC TTCACAACTA TGGTATTGTG CACAGAGACC TCAAACCTGA CAATCTCCTG 
ATTACCTCAA TGGGACACGT CAAACTGACT GACTTTGGCC TGTCCAAGAT TGGTTTAATG 
AGCTTAACAA CTAATCTGTA TGAGGGCCAC ATTGAAAAAG ATACTCGAGA ATTCTTGGAC 
AAACAGGTGT GTGGTACTCC TGAATACATT GCTCCTGAGG TTATTCTGCG ACAGGGTTAT 
GGAAAACCAG TTGACTGGTG GGCCATGGGA GTCATCTTGT ATGAGTTTCT GGTGGGCTGT 
GCTCCTTTCT TTGGAGATAC ACCTGAAGAA CTGTTTGGAC AAGTCATCAG TGATGAGATA 
ATCTGGCCAG AAGGTGATGA AGCTTTGCCT CCGGATGCTC AAGATCTCAT CTCCAAACTT 
CTTCGGCAAA ACCCTCTAGA GCGTCTTGGG ACAGGGAGTG CGTTTGAGGT AAAACAGCAC 
CGCTTCTTTA CTGATCTGGA CTGGAACAGC CTCCTGAGGC AGAAGGCTGA GTTCATTCCT 
CAGCTGGAGT CGGAGGATGA CACTAGCTAC TTTGACACAC GCTCAGATCG TTATCATCAT 
GTGGACTCAG AGGAAGAAGA TGACACAAAT GATGACGACC ATTTGGAGAT CCGTCAGTTC 
TCCTCCTGTT CGCCACGATT CAGCAAGGTG TATAGTAGTA TGGAGCGTCT TTCCTTGCAT 
GAGGAGAAAC GCACCCCTCC TCCTACTAAG CGCAGTCTGA GTGAAGAGGG AGGAGAGCGG 
ATCGACAGTC TAAGTGGCCT AAAGTCCAGA GACCGAAGCT GGTTGGTGGG CTCACCTGAA 
ATTCTCAGAA AGCGTCTCTC TGTTTCCGAG TCTTCTCACA CCGAGAGTGA CTCGAGTCCA 
CCTCTGACTG TTCGTCGCCG CTGCTGCTCG GCCATCATTG AAATGCCTCG CTTCGCCATA 
TCCAGTGAAG AGGATAGCTG TGTGGGGAGC AGGAAGACCC CTGTGAGAGG CCCTCGAGGG 
GATGATCTGC CTCAGGCCAT ACCAGAGCTC CCTGTAGAGA GAGAGCTCAG GCTGGATGAA 
TCGCCCACCA CGCCAGGATC CACTTCCAGT CAAATCAGCC AGTCCACACT CACACCTGGT 
AGTTCTGGTG ATGTGTTAGA TCGTGCTTCT CGCTATAGTG CTGAAGTTTC TGAAAACTCC 
ACTCCAAGAG CCATCAGTGA TTTGGCGGCT CGAAGAGCTC GCCACAGACT GCTTTCAGGA 
GATACAGATA AACACACATC ACGTCCACTT AGCAAAGTCA TCAAATCAGC TTCAGCAACA 
ACCCTGTCAC TCATGATCCC TGCTGATCAC CATGGTGCTT CTCCACTGGC CAGTCCCATG 
TCGCCTCACT CTCTCTCTTC GAACCCCTCA TCACGTGACT CCTCGCCCAG CCGTGACCTT 
TCCCCAGCTG TCTGTAGTGT TAAACCTGCC ATAATCATCC ATCGAGCTGG AAAGAAATAT 
GGTTTTACTT TACGTGCAAT CCGGGTTTAC ATGGGAGACA CAGACATATA TACTGTCCAT 
CACATGGTCT GGCATGTGGA GGATGGAGGA CCTGCTCATG AGGCTGGACT GAGAGAGGGA 
GATCTCATCA CACATGTTAA CGGAGAGCCT GTCCATGGAC TTGTGCACAC AGAAGTTGTT 
GAGCTCATTC TTAAGAGTGG CAGTAAGGTG TCCATCTCTG CTACACCCTT TGAAAATACA 
TCAATTAAGG TTGGTCCTGC TCGCAAAACT AGCTACAAGT CCAAAATGGC CCGTCGCAAC 
AAAAGAACCA AAACTAGAGA AGGACAAGAC AGTAAGAAGA GGAATTCCCT CTTCCGTAAG 
ATCACCAAAC AGGCGACCCT TCTTCACACC AGTCGCAGCC TGTCCTCTCT GAATCGCTCG 
GTCTCTTCTG GTGAGAGCGT CCCAGGCTCT CCTACACACA TGTCACCTCG TTCACCCACA 
CAGGGCTGTC GTTCAACACC TGACTCCGCC CACTCAGTTG GTGGAAACTC ATCCCAAAGT 
AGCTCGCCTA GCTCCAGTGT GCCAAACTCT CCTGCCAGCT CTGGCCAGAT CAGACCCAGC 
TCCTTGCATG GTCTGGCCCC CAAGCTGCAG CGCCAGTACC GATCCCCCCG CCGGAAGTCT 
GCAGGCAACA TCCCTTTATC CCCTCTGGCC CGTACACCAT CGCCCACTCC ACAGAGCACT 
TCTCCCCAGC GTTCCCCTTC TCCCCTGCCA AGCCACGGGC TCATCACTTC TTCAATAGGC 
CAGTCTTTTC CTGTCAAACT GCACTCTTCA CCTCCATTAG TAAGACAGAT ATCTCGGCCA 
AAGAGTGCAG ACCCTCCTCG CTCTCCTCTG CTCAAACGAG TCCAGTCCGC AGAGAAACTC 
GCCGCCTCTC TTTCTTCTTC ATCCTCCTCT CCTTCTCCCT CTTCGGCTGC TGATAAAAAG 
CTTCCAGTTG GTGCCAGCCG CAAACACAGT CTTGATACCT CACATTCAGA GTTTAAAAAA 
GAGATGCTGC AGCGAGACCC TAGTCTACAG AGCCTGCAAG AGTCAGCCAG TGAGACTCTT 
ATGGGAGGAA GAGTGTCTCC TGCAGAAAAG GGAAGCCTGC AGAAAAGTTC AGTGCGTAAA 
CTGGGCAGGC AAGAAGGTCC CGAGTCTGGA ACAGGAACTC TGGGACTTGT TCCTGGAAAG 
AGTAAATTGA AAGACAAGCT GTCTGCCATA CGTGCTGAAC GCCGCGAATC TCTTCAAAAG 
CAGGATGCCA TCCATGAAGT GGATTCATCT GAAGATGAGA CAGACGAAGG GTCGGAAGAC 
AGCCAAGATG GGCGCAGAAC TTGCTATGCT CCTCCTAGCC ATGTCTTGAG GCCTACAACT 
GTAATGGCCT CTCCCCATAC CATCAGAATG GGGCCAGCGG CTCCACCAAC ACTGGGACTT 
TTTCCTTCAA CAGTTGCACC GCCATGCTCA CCTCAGCTCA TCAGAGGACT GCAGATACAA 
AACCAAAGTA CCACTCAATC TCAGACACAA ACTTCAACCT CACAACCAGC GCAAGCTGCA 
GTTAAACCCC CAGAACAGAA ACCAATAACC TCTGTTAAAG ACTCAAGTTC AACGATAATT 
GATGATTCGA AAAAACAGTC ACAGGCACAA GACATTCAGT CCAGACCTTC TCCACATCAA 
CCCAGTCCTC TGTCTAGTAC CTTTTCCACC CAAGGCAGCG CCTTTACCTC TGTTAGTAAA 
GACCCATTTG TCAAGCCCAC CACACCTCCA TTGGATCCCC GGAGGACCCC AAAAATGTCT 
GAGCCAAGGT CAAAAACCAG TGGTAGAACT GATTCTAGCA CTACTTCTGG AGTAGCCAGG 
GATACCCCAG TTGCGACTAC CCCAACAGGA GGAATCACTA AGGAGATGAA GGAGGCTGAC 
AATCGCAGAC AGGCTGCAGC TGCTGCTGCT GCCTCTGCCG CCGCTGCTAC CACCTCTGCA 
TCCACTGCCT CTGAAAGTGG AATGGATAAA GTTGTGTCTC AGCTCGCCAC TGTGGCCAAG 
AGTGTGCTTG GACCTGTCAA GCTCAATATC CCAGGTACTA AAGAGACCAT GGAACGAACA 
AAGGACCAGC GTACCCAGGC AGATGCCACC CACCCAAAGA TAAAAGAATG TCGCCTGGAG 
CACCCTGATT CTCCTGACCA ATTGAGTCCA TCTGCTGCTT CGGGCTCCCA GTCTCCACGA 
CTGACTGAAT CTCCATCTAA ACAAACATCA TCCAAATCTG AGCCAGCCTC AACTTCTCAG 
AGACCTTTAG AGGTCCCAGG GACTTTCAAA AGACAAACTT CTCCAATCCC TCAAGCTAGG 
GAAGAGCATC AGGATAGGTC AGGAACTCTG CAAAGTGGCA CCACATCAAG GCCTAGGTCT 
GAAGCACAAT CACAGACCCA GCAGGCAAAG CCCAGTTCCA CTACGTCACG AGATAAATCC 
AACAAGACAA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_005166291.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166348.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X4, mRNA.

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CloneID	ODa11958
Clone ID Related Accession (Same CDS sequence)	XM_005166291.4 , XM_005166291.3
Accession Version	XM_005166291.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166291.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACT CCTTCTGGAC CGTTCTCTCC AATTTCTCCC TGCCGGAGAG AAGCATGCTC 
CGCCGATCCA AGAGCTGTCG GACGAGCAAC CGAAAAAGTT TGATAGTGAC ATCCAGCACG 
TCTCCAACGC TGCCTCGACC CCATTCACCA CTGCACGGGC ATCCAGGAAG CAGCCCATTG 
GACAGCCCTC GCAATTTCTC CCCCAACACT GCAGCCCACT TCTCTTTTGT ACCAGCTCGA 
AGCCATGGAC ACAGGGCTGA CAGGACTGAC GGTCGTCGCT GGTCTCTCGC CTCTCTGCCG 
TCCTCTGGCT ATGGAACAAA CACCCCCAGT TCGACAGTCT CGTCATCATG TTCATCTCAG 
GAGAAGCTGC ACCAGTTGCC CTTCCAGCCC ACTGCAGACG AGCTGCATTT CCTCACCAAA 
CACTTCAGCT CGGAGAGCAT CACGGATGAG GATGGGAGAC GATCCCCAGC CATGCGTCCG 
CGCTCCCGCA GTCTCAGCCC TGGACGGTCA CCAATATCAT TTGACCATGA GATTGTCATG 
ATGAACCATG TCTACAAGGA GCGTTTTCCT AAGGCCACTG CTCAGATGGA GGTGCGTCTG 
GCCGATTTCA TATCCTCCTC AGCTCCAGAA AAAGTCATGC CTTTAGCTGA TGGCGTCCTG 
AGCTTCATCC ACCACCAGGT TATCGAACTG TCTCGAGATT GTCTGGACAA ATCTCGTGAG 
GGTCTCATCA CCTCCCGCTA CTTCTATGAG CTACAAGAGA ACCTTGAAAA GCTACACCAG 
GATGCTCATG AGCGTTCAGA AAGTGGGGAG GTGACATTTG TCACCCAGCT AGTTAAAAAG 
CTGATGATAA TCATCGCTCG GCCTGCCCGG CTGCTAGAAT GTTTGGAGTT TGACCCGGAG 
GAGTTCTACC ACCTGTTAGA GGCAGCTGAA GGTCATGCTA AAGAAGGTCA AGGCATCAAG 
TCTGACATCC CTCGATACAT CATCAGCCAG CTGGGCCTCA CCAGGGACCC CCTTGAGGAA 
ATGGCCCAGC TGAGCAGCTA TGACAGCGGT AACCCTGAAA CACCAGAGAC AGAGACAGAT 
TCCACAGATA GTCGAGGAGC AACCGTACAG CCACTCCAAC CTCAGCCTCA ACCGCAGCCC 
AAGACTAAAA CACCTCGCGA GGAAGACTTC GAGACCATTA AACTCATCAG TAATGGAGCC 
TATGGGGCTG TGTTTCTGGT GAGGCATAAA GAGACCCGTC AGCGATTTGC CATGAAGAAG 
ATCAACAAGC AGAACCTGAT CCTAAGAAAC CAGATCCAGC AGGCCTTTGT GGAAAGAGAC 
ATCTTGACAT TTGCAGAAAA CCCTTTTGTT GTCAGCATGT TCTGCTCCTT TGAGACAAGG 
AGACATCTTT GCATGGTTAT GGAGTATGTG GAGGGTGGGG ATTGTGCCAC TCTCCTGAAG 
CACATTGGAG CTCTGCCTGT GGATATGGCA CGCATGTACT TTGCTGAAAC CGTTCTTGCA 
TTGGAATACC TTCACAACTA TGGTATTGTG CACAGAGACC TCAAACCTGA CAATCTCCTG 
ATTACCTCAA TGGGACACGT CAAACTGACT GACTTTGGCC TGTCCAAGAT TGGTTTAATG 
AGCTTAACAA CTAATCTGTA TGAGGGCCAC ATTGAAAAAG ATACTCGAGA ATTCTTGGAC 
AAACAGGTGT GTGGTACTCC TGAATACATT GCTCCTGAGG TTATTCTGCG ACAGGGTTAT 
GGAAAACCAG TTGACTGGTG GGCCATGGGA GTCATCTTGT ATGAGTTTCT GGTGGGCTGT 
GCTCCTTTCT TTGGAGATAC ACCTGAAGAA CTGTTTGGAC AAGTCATCAG TGATGAGATA 
ATCTGGCCAG AAGGTGATGA AGCTTTGCCT CCGGATGCTC AAGATCTCAT CTCCAAACTT 
CTTCGGCAAA ACCCTCTAGA GCGTCTTGGG ACAGGGAGTG CGTTTGAGGT AAAACAGCAC 
CGCTTCTTTA CTGATCTGGA CTGGAACAGC CTCCTGAGGC AGAAGGCTGA GTTCATTCCT 
CAGCTGGAGT CGGAGGATGA CACTAGCTAC TTTGACACAC GCTCAGATCG TTATCATCAT 
GTGGACTCAG AGGAAGAAGA TGACACAAAT GATGACGACC ATTTGGAGAT CCGTCAGTTC 
TCCTCCTGTT CGCCACGATT CAGCAAGGTG TATAGTAGTA TGGAGCGTCT TTCCTTGCAT 
GAGGAGAAAC GCACCCCTCC TCCTACTAAG CGCAGTCTGA GTGAAGAGGG AGGAGAGCGG 
ATCGACAGTC TAAGTGGCCT AAAGTCCAGA GACCGAAGCT GGTTGGTGGG CTCACCTGAA 
ATTCTCAGAA AGCGTCTCTC TGTTTCCGAG TCTTCTCACA CCGAGAGTGA CTCGAGTCCA 
CCTCTGACTG TTCGTCGCCG CTGCTGCTCG GCCATCATTG AAATGCCTCG CTTCGCCATA 
TCCAGTGAAG AGGATAGCTG TGTGGGGAGC AGGAAGACCC CTGTGAGAGG CCCTCGAGGG 
GATGATCTGC CTCAGGCCAT ACCAGAGCTC CCTGTAGAGA GAGAGCTCAG GCTGGATGAA 
TCGCCCACCA CGCCAGGATC CACTTCCAGT CAAATCAGCC AGTCCACACT CACACCTGGT 
AGTTCTGGTG ATGTGTTAGA TCGTGCTTCT CGCTATAGTG CTGAAGTTTC TGAAAACTCC 
ACTCCAAGAG CCATCAGTGA TTTGGCGGCT CGAAGAGCTC GCCACAGACT GCTTTCAGGA 
GATACAGATA AACACACATC ACGTCCACTT AGCAAAGTCA TCAAATCAGC TTCAGCAACA 
ACCCTGTCAC TCATGATCCC TGCTGATCAC CATGGTGCTT CTCCACTGGC CAGTCCCATG 
TCGCCTCACT CTCTCTCTTC GAACCCCTCA TCACGTGACT CCTCGCCCAG CCGTGACCTT 
TCCCCAGCTG TCTGTAGTGT TAAACCTGCC ATAATCATCC ATCGAGCTGG AAAGAAATAT 
GGTTTTACTT TACGTGCAAT CCGGGTTTAC ATGGGAGACA CAGACATATA TACTGTCCAT 
CACATGGTCT GGCATGTGGA GGATGGAGGA CCTGCTCATG AGGCTGGACT GAGAGAGGGA 
GATCTCATCA CACATGTTAA CGGAGAGCCT GTCCATGGAC TTGTGCACAC AGAAGTTGTT 
GAGCTCATTC TTAAGAGTGG CAGTAAGGTG TCCATCTCTG CTACACCCTT TGAAAATACA 
TCAATTAAGG TTGGTCCTGC TCGCAAAACT AGCTACAAGT CCAAAATGGC CCGTCGCAAC 
AAAAGAACCA AAACTAGAGA AGGACAAGAC AGTAAGAAGA GGAATTCCCT CTTCCGTAAG 
ATCACCAAAC AGGCGACCCT TCTTCACACC AGTCGCAGCC TGTCCTCTCT GAATCGCTCG 
GTCTCTTCTG GTGAGAGCGT CCCAGGCTCT CCTACACACA TGTCACCTCG TTCACCCACA 
CAGGGCTGTC GTTCAACACC TGACTCCGCC CACTCAGTTG GTGGAAACTC ATCCCAAAGT 
AGCTCGCCTA GCTCCAGTGT GCCAAACTCT CCTGCCAGCT CTGGCCAGAT CAGACCCAGC 
TCCTTGCATG GTCTGGCCCC CAAGCTGCAG CGCCAGTACC GATCCCCCCG CCGGAAGTCT 
GCAGGCAACA TCCCTTTATC CCCTCTGGCC CGTACACCAT CGCCCACTCC ACAGAGCACT 
TCTCCCCAGC GTTCCCCTTC TCCCCTGCCA AGCCACGGGC TCATCACTTC TTCAATAGGC 
CAGTCTTTTC CTGTCAAACT GCACTCTTCA CCTCCATTAG TAAGACAGAT ATCTCGGCCA 
AAGAGTGCAG ACCCTCCTCG CTCTCCTCTG CTCAAACGAG TCCAGTCCGC AGAGAAACTC 
GCCGCCTCTC TTTCTTCTTC ATCCTCCTCT CCTTCTCCCT CTTCGGCTGC TGATAAAAAG 
CTTCCAGTTG GTGCCAGCCG CAAACACAGT CTTGATACCT CACATTCAGA GTTTAAAAAA 
GAGATGCTGC AGCGAGACCC TAGTCTACAG AGCCTGCAAG AGTCAGCCAG TGAGACTCTT 
ATGGGAGGAA GAGTGTCTCC TGCAGAAAAG GGAAGCCTGC AGAAAAGTTC AGTGCGTAAA 
CTGGGCAGGC AAGAAGGTCC CGAGTCTGGA ACAGGAACTC TGGGACTTGT TCCTGGAAAG 
AGTAAATTGA AAGACAAGCT GTCTGCCATA CGTGCTGAAC GCCGCGAATC TCTTCAAAAG 
CAGGATGCCA TCCATGAAGT GGATTCATCT GAAGATGAGA CAGACGAAGG GTCGGAAGAC 
AGCCAAGATG GGCGCAGAAC TTGCTATGCT CCTCCTAGCC ATGTCTTGAG GCCTACAACT 
GTAATGGCCT CTCCCCATAC CATCAGAATG GGGCCAGCGG CTCCACCAAC ACTGGGACTT 
TTTCCTTCAA CAGTTGCACC GCCATGCTCA CCTCAGCTCA TCAGAGGACT GCAGATACAA 
AACCAAAGTA CCACTCAATC TCAGACACAA ACTTCAACCT CACAACCAGC GCAAGCTGCA 
GTTAAACCCC CAGAACAGAA ACCAATAACC TCTGTTAAAG ACTCAAGTTC AACGATAATT 
GATGATTCGA AAAAACAGTC ACAGGCACAA GACATTCAGT CCAGACCTTC TCCACATCAA 
CCCAGTCCTC TGTCTAGTAC CTTTTCCACC CAAGGCAGCG CCTTTACCTC TGTTAGTAAA 
GACCCATTTG TCAAGCCCAC CACACCTCCA TTGGATCCCC GGAGGACCCC AAAAATGTCT 
GAGCCAAGGT CAAAAACCAG TGGTAGAACT GATTCTAGCA CTACTTCTGG AGTAGCCAGG 
GATACCCCAG TTGCGACTAC CCCAACAGGA GGAATCACTA AGGAGATGAA GGAGGCTGAC 
AATCGCAGAC AGGCTGCAGC TGCTGCTGCT GCCTCTGCCG CCGCTGCTAC CACCTCTGCA 
TCCACTGCCT CTGAAAGTGG AATGGATAAA GTTGTGTCTC AGCTCGCCAC TGTGGCCAAG 
AGTGTGCTTG GACCTGTCAA GCTCAATATC CCAGGTACTA AAGAGACCAT GGAACGAACA 
AAGGACCAGC GTACCCAGGC AGATGCCACC CACCCAAAGA TAAAAGAATG TCGCCTGGAG 
CACCCTGATT CTCCTGACCA ATTGAGTCCA TCTGCTGCTT CGGGCTCCCA GTCTCCACGA 
CTGACTGAAT CTCCATCTAA ACAAACATCA TCCAAATCTG AGCCAGCCTC AACTTCTCAG 
AGACCTTTAG AGGTCCCAGG GACTTTCAAA AGACAAACTT CTCCAATCCC TCAAGCTAGG 
GAAGAGCATC AGGATAGGTC AGGAACTCTG CAAAGTGGCA CCACATCAAG GCCTAGGTCT 
GAAGCACAAT CACAGACCCA GCAGGCAAAG CCCAGTTCCA CTACGTCACG AGATAAATCC 
AACAAGACAA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166348.1
CDS	149..5623
Translation	MSDSFWTVLSNFSLPERSMLRRSKSCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYAPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPCSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_005166291.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166291.3

ORF Insert Sequence:

ATGTCTGACT CCTTCTGGAC CGTTCTCTCC AATTTCTCCC TGCCGGAGAG AAGCATGCTC 
CGCCGATCCA AGAGCTGTCG GACGAGCAAC CGAAAAAGTT TGATAGTGAC ATCCAGCACG 
TCTCCAACGC TGCCTCGACC CCATTCACCA CTGCACGGGC ATCCAGGAAG CAGCCCATTG 
GACAGCCCTC GCAATTTCTC CCCCAACACT GCAGCCCACT TCTCTTTTGT ACCAGCTCGA 
AGCCATGGAC ACAGGGCTGA CAGGACTGAC GGTCGTCGCT GGTCTCTCGC CTCTCTGCCG 
TCCTCTGGCT ATGGAACAAA CACCCCCAGT TCGACAGTCT CGTCATCATG TTCATCTCAG 
GAGAAGCTGC ACCAGTTGCC CTTCCAGCCC ACTGCAGACG AGCTGCATTT CCTCACCAAA 
CACTTCAGCT CGGAGAGCAT CACGGATGAG GATGGGAGAC GATCCCCAGC CATGCGTCCG 
CGCTCCCGCA GTCTCAGCCC TGGACGGTCA CCAATATCAT TTGACCATGA GATTGTCATG 
ATGAACCATG TCTACAAGGA GCGTTTTCCT AAGGCCACTG CTCAGATGGA GGTGCGTCTG 
GCCGATTTCA TATCCTCCTC AGCTCCAGAA AAAGTCATGC CTTTAGCTGA TGGCGTCCTG 
AGCTTCATCC ACCACCAGGT TATCGAACTG TCTCGAGATT GTCTGGACAA ATCTCGTGAG 
GGTCTCATCA CCTCCCGCTA CTTCTATGAG CTACAAGAGA ACCTTGAAAA GCTACACCAG 
GATGCTCATG AGCGTTCAGA AAGTGGGGAG GTGACATTTG TCACCCAGCT AGTTAAAAAG 
CTGATGATAA TCATCGCTCG GCCTGCCCGG CTGCTAGAAT GTTTGGAGTT TGACCCGGAG 
GAGTTCTACC ACCTGTTAGA GGCAGCTGAA GGTCATGCTA AAGAAGGTCA AGGCATCAAG 
TCTGACATCC CTCGATACAT CATCAGCCAG CTGGGCCTCA CCAGGGACCC CCTTGAGGAA 
ATGGCCCAGC TGAGCAGCTA TGACAGCGGT AACCCTGAAA CACCAGAGAC AGAGACAGAT 
TCCACAGATA GTCGAGGAGC AACCGTACAG CCACTCCAAC CTCAGCCTCA ACCGCAGCCC 
AAGACTAAAA CACCTCGCGA GGAAGACTTC GAGACCATTA AACTCATCAG TAATGGAGCC 
TATGGGGCTG TGTTTCTGGT GAGGCATAAA GAGACCCGTC AGCGATTTGC CATGAAGAAG 
ATCAACAAGC AGAACCTGAT CCTAAGAAAC CAGATCCAGC AGGCCTTTGT GGAAAGAGAC 
ATCTTGACAT TTGCAGAAAA CCCTTTTGTT GTCAGCATGT TCTGCTCCTT TGAGACAAGG 
AGACATCTTT GCATGGTTAT GGAGTATGTG GAGGGTGGGG ATTGTGCCAC TCTCCTGAAG 
CACATTGGAG CTCTGCCTGT GGATATGGCA CGCATGTACT TTGCTGAAAC CGTTCTTGCA 
TTGGAATACC TTCACAACTA TGGTATTGTG CACAGAGACC TCAAACCTGA CAATCTCCTG 
ATTACCTCAA TGGGACACGT CAAACTGACT GACTTTGGCC TGTCCAAGAT TGGTTTAATG 
AGCTTAACAA CTAATCTGTA TGAGGGCCAC ATTGAAAAAG ATACTCGAGA ATTCTTGGAC 
AAACAGGTGT GTGGTACTCC TGAATACATT GCTCCTGAGG TTATTCTGCG ACAGGGTTAT 
GGAAAACCAG TTGACTGGTG GGCCATGGGA GTCATCTTGT ATGAGTTTCT GGTGGGCTGT 
GCTCCTTTCT TTGGAGATAC ACCTGAAGAA CTGTTTGGAC AAGTCATCAG TGATGAGATA 
ATCTGGCCAG AAGGTGATGA AGCTTTGCCT CCGGATGCTC AAGATCTCAT CTCCAAACTT 
CTTCGGCAAA ACCCTCTAGA GCGTCTTGGG ACAGGGAGTG CGTTTGAGGT AAAACAGCAC 
CGCTTCTTTA CTGATCTGGA CTGGAACAGC CTCCTGAGGC AGAAGGCTGA GTTCATTCCT 
CAGCTGGAGT CGGAGGATGA CACTAGCTAC TTTGACACAC GCTCAGATCG TTATCATCAT 
GTGGACTCAG AGGAAGAAGA TGACACAAAT GATGACGACC ATTTGGAGAT CCGTCAGTTC 
TCCTCCTGTT CGCCACGATT CAGCAAGGTG TATAGTAGTA TGGAGCGTCT TTCCTTGCAT 
GAGGAGAAAC GCACCCCTCC TCCTACTAAG CGCAGTCTGA GTGAAGAGGG AGGAGAGCGG 
ATCGACAGTC TAAGTGGCCT AAAGTCCAGA GACCGAAGCT GGTTGGTGGG CTCACCTGAA 
ATTCTCAGAA AGCGTCTCTC TGTTTCCGAG TCTTCTCACA CCGAGAGTGA CTCGAGTCCA 
CCTCTGACTG TTCGTCGCCG CTGCTGCTCG GCCATCATTG AAATGCCTCG CTTCGCCATA 
TCCAGTGAAG AGGATAGCTG TGTGGGGAGC AGGAAGACCC CTGTGAGAGG CCCTCGAGGG 
GATGATCTGC CTCAGGCCAT ACCAGAGCTC CCTGTAGAGA GAGAGCTCAG GCTGGATGAA 
TCGCCCACCA CGCCAGGATC CACTTCCAGT CAAATCAGCC AGTCCACACT CACACCTGGT 
AGTTCTGGTG ATGTGTTAGA TCGTGCTTCT CGCTATAGTG CTGAAGTTTC TGAAAACTCC 
ACTCCAAGAG CCATCAGTGA TTTGGCGGCT CGAAGAGCTC GCCACAGACT GCTTTCAGGA 
GATACAGATA AACACACATC ACGTCCACTT AGCAAAGTCA TCAAATCAGC TTCAGCAACA 
ACCCTGTCAC TCATGATCCC TGCTGATCAC CATGGTGCTT CTCCACTGGC CAGTCCCATG 
TCGCCTCACT CTCTCTCTTC GAACCCCTCA TCACGTGACT CCTCGCCCAG CCGTGACCTT 
TCCCCAGCTG TCTGTAGTGT TAAACCTGCC ATAATCATCC ATCGAGCTGG AAAGAAATAT 
GGTTTTACTT TACGTGCAAT CCGGGTTTAC ATGGGAGACA CAGACATATA TACTGTCCAT 
CACATGGTCT GGCATGTGGA GGATGGAGGA CCTGCTCATG AGGCTGGACT GAGAGAGGGA 
GATCTCATCA CACATGTTAA CGGAGAGCCT GTCCATGGAC TTGTGCACAC AGAAGTTGTT 
GAGCTCATTC TTAAGAGTGG CAGTAAGGTG TCCATCTCTG CTACACCCTT TGAAAATACA 
TCAATTAAGG TTGGTCCTGC TCGCAAAACT AGCTACAAGT CCAAAATGGC CCGTCGCAAC 
AAAAGAACCA AAACTAGAGA AGGACAAGAC AGTAAGAAGA GGAATTCCCT CTTCCGTAAG 
ATCACCAAAC AGGCGACCCT TCTTCACACC AGTCGCAGCC TGTCCTCTCT GAATCGCTCG 
GTCTCTTCTG GTGAGAGCGT CCCAGGCTCT CCTACACACA TGTCACCTCG TTCACCCACA 
CAGGGCTGTC GTTCAACACC TGACTCCGCC CACTCAGTTG GTGGAAACTC ATCCCAAAGT 
AGCTCGCCTA GCTCCAGTGT GCCAAACTCT CCTGCCAGCT CTGGCCAGAT CAGACCCAGC 
TCCTTGCATG GTCTGGCCCC CAAGCTGCAG CGCCAGTACC GATCCCCCCG CCGGAAGTCT 
GCAGGCAACA TCCCTTTATC CCCTCTGGCC CGTACACCAT CGCCCACTCC ACAGAGCACT 
TCTCCCCAGC GTTCCCCTTC TCCCCTGCCA AGCCACGGGC TCATCACTTC TTCAATAGGC 
CAGTCTTTTC CTGTCAAACT GCACTCTTCA CCTCCATTAG TAAGACAGAT ATCTCGGCCA 
AAGAGTGCAG ACCCTCCTCG CTCTCCTCTG CTCAAACGAG TCCAGTCCGC AGAGAAACTC 
GCCGCCTCTC TTTCTTCTTC ATCCTCCTCT CCTTCTCCCT CTTCGGCTGC TGATAAAAAG 
CTTCCAGTTG GTGCCAGCCG CAAACACAGT CTTGATACCT CACATTCAGA GTTTAAAAAA 
GAGATGCTGC AGCGAGACCC TAGTCTACAG AGCCTGCAAG AGTCAGCCAG TGAGACTCTT 
ATGGGAGGAA GAGTGTCTCC TGCAGAAAAG GGAAGCCTGC AGAAAAGTTC AGTGCGTAAA 
CTGGGCAGGC AAGAAGGTCC CGAGTCTGGA ACAGGAACTC TGGGACTTGT TCCTGGAAAG 
AGTAAATTGA AAGACAAGCT GTCTGCCATA CGTGCTGAAC GCCGCGAATC TCTTCAAAAG 
CAGGATGCCA TCCATGAAGT GGATTCATCT GAAGATGAGA CAGACGAAGG GTCGGAAGAC 
AGCCAAGATG GGCGCAGAAC TTGCTATGCT CCTCCTAGCC ATGTCTTGAG GCCTACAACT 
GTAATGGCCT CTCCCCATAC CATCAGAATG GGGCCAGCGG CTCCACCAAC ACTGGGACTT 
TTTCCTTCAA CAGTTGCACC GCCATGCTCA CCTCAGCTCA TCAGAGGACT GCAGATACAA 
AACCAAAGTA CCACTCAATC TCAGACACAA ACTTCAACCT CACAACCAGC GCAAGCTGCA 
GTTAAACCCC CAGAACAGAA ACCAATAACC TCTGTTAAAG ACTCAAGTTC AACGATAATT 
GATGATTCGA AAAAACAGTC ACAGGCACAA GACATTCAGT CCAGACCTTC TCCACATCAA 
CCCAGTCCTC TGTCTAGTAC CTTTTCCACC CAAGGCAGCG CCTTTACCTC TGTTAGTAAA 
GACCCATTTG TCAAGCCCAC CACACCTCCA TTGGATCCCC GGAGGACCCC AAAAATGTCT 
GAGCCAAGGT CAAAAACCAG TGGTAGAACT GATTCTAGCA CTACTTCTGG AGTAGCCAGG 
GATACCCCAG TTGCGACTAC CCCAACAGGA GGAATCACTA AGGAGATGAA GGAGGCTGAC 
AATCGCAGAC AGGCTGCAGC TGCTGCTGCT GCCTCTGCCG CCGCTGCTAC CACCTCTGCA 
TCCACTGCCT CTGAAAGTGG AATGGATAAA GTTGTGTCTC AGCTCGCCAC TGTGGCCAAG 
AGTGTGCTTG GACCTGTCAA GCTCAATATC CCAGGTACTA AAGAGACCAT GGAACGAACA 
AAGGACCAGC GTACCCAGGC AGATGCCACC CACCCAAAGA TAAAAGAATG TCGCCTGGAG 
CACCCTGATT CTCCTGACCA ATTGAGTCCA TCTGCTGCTT CGGGCTCCCA GTCTCCACGA 
CTGACTGAAT CTCCATCTAA ACAAACATCA TCCAAATCTG AGCCAGCCTC AACTTCTCAG 
AGACCTTTAG AGGTCCCAGG GACTTTCAAA AGACAAACTT CTCCAATCCC TCAAGCTAGG 
GAAGAGCATC AGGATAGGTC AGGAACTCTG CAAAGTGGCA CCACATCAAG GCCTAGGTCT 
GAAGCACAAT CACAGACCCA GCAGGCAAAG CCCAGTTCCA CTACGTCACG AGATAAATCC 
AACAAGACAA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_009302494.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300769.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X4, mRNA.

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CloneID	ODa47560
Clone ID Related Accession (Same CDS sequence)	XM_009302494.3 , XM_009302494.2
Accession Version	XM_009302494.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302494.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAA CCGAGAGCAA AAACAAAAGG AAACTCTGCA CATCTGAGCA GCAGGACAGC 
AGCACTGAAG GAAAAGACGA GGAGCTGCCG GCGCCGCCTC CCCTGCTGTT CAGGAAGCTG 
AGTAACCCAG ACCTGTCTCC TGCCGCAGGA AAAACCAAAC TGCAGAGGCA GCTCAGTCAG 
GATGACAGCA GAGGCCGACG CAGCAGCATG GCTGGCATCC TCACTGGAAA GCAGCTTCTG 
CCTCTGTCCA GCAGTATGCA TGGAGGAGTG AGTCAGCTGG CCTGGCAGCA GCACACAGGG 
GAGCCCAATA ACTTGGTCCG CATGAGGAGT CAGTCCTTTG GCCAGTCTGC CCCGTCTCTC 
ACTGCGGGAC TGAAGGAGCT GAGTCTTCCT AGAAGAGGCA GTTTCTGTCG GACGAGCAAC 
CGAAAAAGTT TGATAGTGAC ATCCAGCACG TCTCCAACGC TGCCTCGACC CCATTCACCA 
CTGCACGGGC ATCCAGGAAG CAGCCCATTG GACAGCCCTC GCAATTTCTC CCCCAACACT 
GCAGCCCACT TCTCTTTTGT ACCAGCTCGA AGCCATGGAC ACAGGGCTGA CAGGACTGAC 
GGTCGTCGCT GGTCTCTCGC CTCTCTGCCG TCCTCTGGCT ATGGAACAAA CACCCCCAGT 
TCGACAGTCT CGTCATCATG TTCATCTCAG GAGAAGCTGC ACCAGTTGCC CTTCCAGCCC 
ACTGCAGACG AGCTGCATTT CCTCACCAAA CACTTCAGCT CGGAGAGCAT CACGGATGAG 
GATGGGAGAC GATCCCCAGC CATGCGTCCG CGCTCCCGCA GTCTCAGCCC TGGACGGTCA 
CCAATATCAT TTGACCATGA GATTGTCATG ATGAACCATG TCTACAAGGA GCGTTTTCCT 
AAGGCCACTG CTCAGATGGA GGTGCGTCTG GCCGATTTCA TATCCTCCTC AGCTCCAGAA 
AAAGTCATGC CTTTAGCTGA TGGCGTCCTG AGCTTCATCC ACCACCAGGT TATCGAACTG 
TCTCGAGATT GTCTGGACAA ATCTCGTGAG GGTCTCATCA CCTCCCGCTA CTTCTATGAG 
CTACAAGAGA ACCTTGAAAA GCTACACCAG GATGCTCATG AGCGTTCAGA AAGTGGGGAG 
GTGACATTTG TCACCCAGCT AGTTAAAAAG CTGATGATAA TCATCGCTCG GCCTGCCCGG 
CTGCTAGAAT GTTTGGAGTT TGACCCGGAG GAGTTCTACC ACCTGTTAGA GGCAGCTGAA 
GGTCATGCTA AAGAAGGTCA AGGCATCAAG TCTGACATCC CTCGATACAT CATCAGCCAG 
CTGGGCCTCA CCAGGGACCC CCTTGAGGAA ATGGCCCAGC TGAGCAGCTA TGACAGCGGT 
AACCCTGAAA CACCAGAGAC AGAGACAGAT TCCACAGATA GTCGAGGAGC AACCGTACAG 
CCACTCCAAC CTCAGCCTCA ACCGCAGCCC AAGACTAAAA CACCTCGCGA GGAAGACTTC 
GAGACCATTA AACTCATCAG TAATGGAGCC TATGGGGCTG TGTTTCTGGT GAGGCATAAA 
GAGACCCGTC AGCGATTTGC CATGAAGAAG ATCAACAAGC AGAACCTGAT CCTAAGAAAC 
CAGATCCAGC AGGCCTTTGT GGAAAGAGAC ATCTTGACAT TTGCAGAAAA CCCTTTTGTT 
GTCAGCATGT TCTGCTCCTT TGAGACAAGG AGACATCTTT GCATGGTTAT GGAGTATGTG 
GAGGGTGGGG ATTGTGCCAC TCTCCTGAAG CACATTGGAG CTCTGCCTGT GGATATGGCA 
CGCATGTACT TTGCTGAAAC CGTTCTTGCA TTGGAATACC TTCACAACTA TGGTATTGTG 
CACAGAGACC TCAAACCTGA CAATCTCCTG ATTACCTCAA TGGGACACGT CAAACTGACT 
GACTTTGGCC TGTCCAAGAT TGGTTTAATG AGCTTAACAA CTAATCTGTA TGAGGGCCAC 
ATTGAAAAAG ATACTCGAGA ATTCTTGGAC AAACAGGTGT GTGGTACTCC TGAATACATT 
GCTCCTGAGG TTATTCTGCG ACAGGGTTAT GGAAAACCAG TTGACTGGTG GGCCATGGGA 
GTCATCTTGT ATGAGTTTCT GGTGGGCTGT GCTCCTTTCT TTGGAGATAC ACCTGAAGAA 
CTGTTTGGAC AAGTCATCAG TGATGAGATA ATCTGGCCAG AAGGTGATGA AGCTTTGCCT 
CCGGATGCTC AAGATCTCAT CTCCAAACTT CTTCGGCAAA ACCCTCTAGA GCGTCTTGGG 
ACAGGGAGTG CGTTTGAGGT AAAACAGCAC CGCTTCTTTA CTGATCTGGA CTGGAACAGC 
CTCCTGAGGC AGAAGGCTGA GTTCATTCCT CAGCTGGAGT CGGAGGATGA CACTAGCTAC 
TTTGACACAC GCTCAGATCG TTATCATCAT GTGGACTCAG AGGAAGAAGA TGACACAAAT 
GATGACGACC ATTTGGAGAT CCGTCAGTTC TCCTCCTGTT CGCCACGATT CAGCAAGGTG 
TATAGTAGTA TGGAGCGTCT TTCCTTGCAT GAGGAGAAAC GCACCCCTCC TCCTACTAAG 
CGCAGTCTGA GTGAAGAGGG AGGAGAGCGG ATCGACAGTC TAAGTGGCCT AAAGTCCAGA 
GACCGAAGCT GGTTGGTGGG CTCACCTGAA ATTCTCAGAA AGCGTCTCTC TGTTTCCGAG 
TCTTCTCACA CCGAGAGTGA CTCGAGTCCA CCTCTGACTG TTCGTCGCCG CTGCTGCTCG 
GCCATCATTG AAATGCCTCG CTTCGCCATA TCCAGTGAAG AGGATAGCTG TGTGGGGAGC 
AGGAAGACCC CTGTGAGAGG CCCTCGAGGG GATGATCTGC CTCAGGCCAT ACCAGAGCTC 
CCTGTAGAGA GAGAGCTCAG GCTGGATGAA TCGCCCACCA CGCCAGGATC CACTTCCAGT 
CAAATCAGCC AGTCCACACT CACACCTGGT AGTTCTGGTG ATGTGTTAGA TCGTGCTTCT 
CGCTATAGTG CTGAAGTTTC TGAAAACTCC ACTCCAAGAG CCATCAGTGA TTTGGCGGCT 
CGAAGAGCTC GCCACAGACT GCTTTCAGGA GATACAGATA AACACACATC ACGTCCACTT 
AGCAAAGTCA TCAAATCAGC TTCAGCAACA ACCCTGTCAC TCATGATCCC TGCTGATCAC 
CATGGTGCTT CTCCACTGGC CAGTCCCATG TCGCCTCACT CTCTCTCTTC GAACCCCTCA 
TCACGTGACT CCTCGCCCAG CCGTGACCTT TCCCCAGCTG TCTGTAGTGT TAAACCTGCC 
ATAATCATCC ATCGAGCTGG AAAGAAATAT GGTTTTACTT TACGTGCAAT CCGGGTTTAC 
ATGGGAGACA CAGACATATA TACTGTCCAT CACATGGTCT GGCATGTGGA GGATGGAGGA 
CCTGCTCATG AGGCTGGACT GAGAGAGGGA GATCTCATCA CACATGTTAA CGGAGAGCCT 
GTCCATGGAC TTGTGCACAC AGAAGTTGTT GAGCTCATTC TTAAGAGTGG CAGTAAGGTG 
TCCATCTCTG CTACACCCTT TGAAAATACA TCAATTAAGG TTGGTCCTGC TCGCAAAACT 
AGCTACAAGT CCAAAATGGC CCGTCGCAAC AAAAGAACCA AAACTAGAGA AGGACAAGAC 
AGTAAGAAGA GGAATTCCCT CTTCCGTAAG ATCACCAAAC AGGCGACCCT TCTTCACACC 
AGTCGCAGCC TGTCCTCTCT GAATCGCTCG GTCTCTTCTG GTGAGAGCGT CCCAGGCTCT 
CCTACACACA TGTCACCTCG TTCACCCACA CAGGGCTGTC GTTCAACACC TGACTCCGCC 
CACTCAGTTG GTGGAAACTC ATCCCAAAGT AGCTCGCCTA GCTCCAGTGT GCCAAACTCT 
CCTGCCAGCT CTGGCCAGAT CAGACCCAGC TCCTTGCATG GTCTGGCCCC CAAGCTGCAG 
CGCCAGTACC GATCCCCCCG CCGGAAGTCT GCAGGCAACA TCCCTTTATC CCCTCTGGCC 
CGTACACCAT CGCCCACTCC ACAGAGCACT TCTCCCCAGC GTTCCCCTTC TCCCCTGCCA 
AGCCACGGGC TCATCACTTC TTCAATAGGC CAGTCTTTTC CTGTCAAACT GCACTCTTCA 
CCTCCATTAG TAAGACAGAT ATCTCGGCCA AAGAGTGCAG ACCCTCCTCG CTCTCCTCTG 
CTCAAACGAG TCCAGTCCGC AGAGAAACTC GCCGCCTCTC TTTCTTCTTC ATCCTCCTCT 
CCTTCTCCCT CTTCGGCTGC TGATAAAAAG CTTCCAGTTG GTGCCAGCCG CAAACACAGT 
CTTGATACCT CACATTCAGA GTTTAAAAAA GAGATGCTGC AGCGAGACCC TAGTCTACAG 
AGCCTGCAAG AGTCAGCCAG TGAGACTCTT ATGGGAGGAA GAGTGTCTCC TGCAGAAAAG 
GGAAGCCTGC AGAAAAGTTC AGTGCGTAAA CTGGGCAGGC AAGAAGGTCC CGAGTCTGGA 
ACAGGAACTC TGGGACTTGT TCCTGGAAAG AGTAAATTGA AAGACAAGCT GTCTGCCATA 
CGTGCTGAAC GCCGCGAATC TCTTCAAAAG CAGGATGCCA TCCATGAAGT GGATTCATCT 
GAAGATGAGA CAGACGAAGG GTCGGAAGAC AGCCAAGATG GGCGCAGAAC TTGCTATGCT 
CCTCCTAGCC ATGTCTTGAG GCCTACAACT GTAATGGCCT CTCCCCATAC CATCAGAATG 
GGGCCAGCGG CTCCACCAAC ACTGGGACTT TTTCCTTCAA CAGTTGCACC GCCATGCTCA 
CCTCAGCTCA TCAGAGGACT GCAGATACAA AACCAAAGTA CCACTCAATC TCAGACACAA 
ACTTCAACCT CACAACCAGC GCAAGCTGCA GTTAAACCCC CAGAACAGAA ACCAATAACC 
TCTGTTAAAG ACTCAAGTTC AACGATAATT GATGATTCGA AAAAACAGTC ACAGGCACAA 
GACATTCAGT CCAGACCTTC TCCACATCAA CCCAGTCCTC TGTCTAGTAC CTTTTCCACC 
CAAGGCAGCG CCTTTACCTC TGTTAGTAAA GACCCATTTG TCAAGCCCAC CACACCTCCA 
TTGGATCCCC GGAGGACCCC AAAAATGTCT GAGCCAAGGT CAAAAACCAG TGGTAGAACT 
GATTCTAGCA CTACTTCTGG AGTAGCCAGG GATACCCCAG TTGCGACTAC CCCAACAGGA 
GGAATCACTA AGGAGATGAA GGAGGCTGAC AATCGCAGAC AGGCTGCAGC TGCTGCTGCT 
GCCTCTGCCG CCGCTGCTAC CACCTCTGCA TCCACTGCCT CTGAAAGTGG AATGGATAAA 
GTTGTGTCTC AGCTCGCCAC TGTGGCCAAG AGTGTGCTTG GACCTGTCAA GCTCAATATC 
CCAGGTACTA AAGAGACCAT GGAACGAACA AAGGACCAGC GTACCCAGGC AGATGCCACC 
CACCCAAAGA TAAAAGAATG TCGCCTGGAG CACCCTGATT CTCCTGACCA ATTGAGTCCA 
TCTGCTGCTT CGGGCTCCCA GTCTCCACGA CTGACTGAAT CTCCATCTAA ACAAACATCA 
TCCAAATCTG AGCCAGCCTC AACTTCTCAG AGACCTTTAG AGGTCCCAGG GACTTTCAAA 
AGACAAACTT CTCCAATCCC TCAAGCTAGG GAAGAGCATC AGGATAGGTC AGGAACTCTG 
CAAAGTGGCA CCACATCAAG GCCTAGGTCT GAAGCACAAT CACAGACCCA GCAGGCAAAG 
CCCAGTTCCA CTACGTCACG AGATAAATCC AACAAGACAA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300769.1
CDS	1056..6860
Translation	MKKTESKNKRKLCTSEQQDSSTEGKDEELPAPPPLLFRKLSNPDLSPAAGKTKLQRQLSQDDSRGRRSSMAGILTGKQLLPLSSSMHGGVSQLAWQQHTGEPNNLVRMRSQSFGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYAPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPCSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_009302494.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302494.3

ORF Insert Sequence:

ATGAAGAAAA CCGAGAGCAA AAACAAAAGG AAACTCTGCA CATCTGAGCA GCAGGACAGC 
AGCACTGAAG GAAAAGACGA GGAGCTGCCG GCGCCGCCTC CCCTGCTGTT CAGGAAGCTG 
AGTAACCCAG ACCTGTCTCC TGCCGCAGGA AAAACCAAAC TGCAGAGGCA GCTCAGTCAG 
GATGACAGCA GAGGCCGACG CAGCAGCATG GCTGGCATCC TCACTGGAAA GCAGCTTCTG 
CCTCTGTCCA GCAGTATGCA TGGAGGAGTG AGTCAGCTGG CCTGGCAGCA GCACACAGGG 
GAGCCCAATA ACTTGGTCCG CATGAGGAGT CAGTCCTTTG GCCAGTCTGC CCCGTCTCTC 
ACTGCGGGAC TGAAGGAGCT GAGTCTTCCT AGAAGAGGCA GTTTCTGTCG GACGAGCAAC 
CGAAAAAGTT TGATAGTGAC ATCCAGCACG TCTCCAACGC TGCCTCGACC CCATTCACCA 
CTGCACGGGC ATCCAGGAAG CAGCCCATTG GACAGCCCTC GCAATTTCTC CCCCAACACT 
GCAGCCCACT TCTCTTTTGT ACCAGCTCGA AGCCATGGAC ACAGGGCTGA CAGGACTGAC 
GGTCGTCGCT GGTCTCTCGC CTCTCTGCCG TCCTCTGGCT ATGGAACAAA CACCCCCAGT 
TCGACAGTCT CGTCATCATG TTCATCTCAG GAGAAGCTGC ACCAGTTGCC CTTCCAGCCC 
ACTGCAGACG AGCTGCATTT CCTCACCAAA CACTTCAGCT CGGAGAGCAT CACGGATGAG 
GATGGGAGAC GATCCCCAGC CATGCGTCCG CGCTCCCGCA GTCTCAGCCC TGGACGGTCA 
CCAATATCAT TTGACCATGA GATTGTCATG ATGAACCATG TCTACAAGGA GCGTTTTCCT 
AAGGCCACTG CTCAGATGGA GGTGCGTCTG GCCGATTTCA TATCCTCCTC AGCTCCAGAA 
AAAGTCATGC CTTTAGCTGA TGGCGTCCTG AGCTTCATCC ACCACCAGGT TATCGAACTG 
TCTCGAGATT GTCTGGACAA ATCTCGTGAG GGTCTCATCA CCTCCCGCTA CTTCTATGAG 
CTACAAGAGA ACCTTGAAAA GCTACACCAG GATGCTCATG AGCGTTCAGA AAGTGGGGAG 
GTGACATTTG TCACCCAGCT AGTTAAAAAG CTGATGATAA TCATCGCTCG GCCTGCCCGG 
CTGCTAGAAT GTTTGGAGTT TGACCCGGAG GAGTTCTACC ACCTGTTAGA GGCAGCTGAA 
GGTCATGCTA AAGAAGGTCA AGGCATCAAG TCTGACATCC CTCGATACAT CATCAGCCAG 
CTGGGCCTCA CCAGGGACCC CCTTGAGGAA ATGGCCCAGC TGAGCAGCTA TGACAGCGGT 
AACCCTGAAA CACCAGAGAC AGAGACAGAT TCCACAGATA GTCGAGGAGC AACCGTACAG 
CCACTCCAAC CTCAGCCTCA ACCGCAGCCC AAGACTAAAA CACCTCGCGA GGAAGACTTC 
GAGACCATTA AACTCATCAG TAATGGAGCC TATGGGGCTG TGTTTCTGGT GAGGCATAAA 
GAGACCCGTC AGCGATTTGC CATGAAGAAG ATCAACAAGC AGAACCTGAT CCTAAGAAAC 
CAGATCCAGC AGGCCTTTGT GGAAAGAGAC ATCTTGACAT TTGCAGAAAA CCCTTTTGTT 
GTCAGCATGT TCTGCTCCTT TGAGACAAGG AGACATCTTT GCATGGTTAT GGAGTATGTG 
GAGGGTGGGG ATTGTGCCAC TCTCCTGAAG CACATTGGAG CTCTGCCTGT GGATATGGCA 
CGCATGTACT TTGCTGAAAC CGTTCTTGCA TTGGAATACC TTCACAACTA TGGTATTGTG 
CACAGAGACC TCAAACCTGA CAATCTCCTG ATTACCTCAA TGGGACACGT CAAACTGACT 
GACTTTGGCC TGTCCAAGAT TGGTTTAATG AGCTTAACAA CTAATCTGTA TGAGGGCCAC 
ATTGAAAAAG ATACTCGAGA ATTCTTGGAC AAACAGGTGT GTGGTACTCC TGAATACATT 
GCTCCTGAGG TTATTCTGCG ACAGGGTTAT GGAAAACCAG TTGACTGGTG GGCCATGGGA 
GTCATCTTGT ATGAGTTTCT GGTGGGCTGT GCTCCTTTCT TTGGAGATAC ACCTGAAGAA 
CTGTTTGGAC AAGTCATCAG TGATGAGATA ATCTGGCCAG AAGGTGATGA AGCTTTGCCT 
CCGGATGCTC AAGATCTCAT CTCCAAACTT CTTCGGCAAA ACCCTCTAGA GCGTCTTGGG 
ACAGGGAGTG CGTTTGAGGT AAAACAGCAC CGCTTCTTTA CTGATCTGGA CTGGAACAGC 
CTCCTGAGGC AGAAGGCTGA GTTCATTCCT CAGCTGGAGT CGGAGGATGA CACTAGCTAC 
TTTGACACAC GCTCAGATCG TTATCATCAT GTGGACTCAG AGGAAGAAGA TGACACAAAT 
GATGACGACC ATTTGGAGAT CCGTCAGTTC TCCTCCTGTT CGCCACGATT CAGCAAGGTG 
TATAGTAGTA TGGAGCGTCT TTCCTTGCAT GAGGAGAAAC GCACCCCTCC TCCTACTAAG 
CGCAGTCTGA GTGAAGAGGG AGGAGAGCGG ATCGACAGTC TAAGTGGCCT AAAGTCCAGA 
GACCGAAGCT GGTTGGTGGG CTCACCTGAA ATTCTCAGAA AGCGTCTCTC TGTTTCCGAG 
TCTTCTCACA CCGAGAGTGA CTCGAGTCCA CCTCTGACTG TTCGTCGCCG CTGCTGCTCG 
GCCATCATTG AAATGCCTCG CTTCGCCATA TCCAGTGAAG AGGATAGCTG TGTGGGGAGC 
AGGAAGACCC CTGTGAGAGG CCCTCGAGGG GATGATCTGC CTCAGGCCAT ACCAGAGCTC 
CCTGTAGAGA GAGAGCTCAG GCTGGATGAA TCGCCCACCA CGCCAGGATC CACTTCCAGT 
CAAATCAGCC AGTCCACACT CACACCTGGT AGTTCTGGTG ATGTGTTAGA TCGTGCTTCT 
CGCTATAGTG CTGAAGTTTC TGAAAACTCC ACTCCAAGAG CCATCAGTGA TTTGGCGGCT 
CGAAGAGCTC GCCACAGACT GCTTTCAGGA GATACAGATA AACACACATC ACGTCCACTT 
AGCAAAGTCA TCAAATCAGC TTCAGCAACA ACCCTGTCAC TCATGATCCC TGCTGATCAC 
CATGGTGCTT CTCCACTGGC CAGTCCCATG TCGCCTCACT CTCTCTCTTC GAACCCCTCA 
TCACGTGACT CCTCGCCCAG CCGTGACCTT TCCCCAGCTG TCTGTAGTGT TAAACCTGCC 
ATAATCATCC ATCGAGCTGG AAAGAAATAT GGTTTTACTT TACGTGCAAT CCGGGTTTAC 
ATGGGAGACA CAGACATATA TACTGTCCAT CACATGGTCT GGCATGTGGA GGATGGAGGA 
CCTGCTCATG AGGCTGGACT GAGAGAGGGA GATCTCATCA CACATGTTAA CGGAGAGCCT 
GTCCATGGAC TTGTGCACAC AGAAGTTGTT GAGCTCATTC TTAAGAGTGG CAGTAAGGTG 
TCCATCTCTG CTACACCCTT TGAAAATACA TCAATTAAGG TTGGTCCTGC TCGCAAAACT 
AGCTACAAGT CCAAAATGGC CCGTCGCAAC AAAAGAACCA AAACTAGAGA AGGACAAGAC 
AGTAAGAAGA GGAATTCCCT CTTCCGTAAG ATCACCAAAC AGGCGACCCT TCTTCACACC 
AGTCGCAGCC TGTCCTCTCT GAATCGCTCG GTCTCTTCTG GTGAGAGCGT CCCAGGCTCT 
CCTACACACA TGTCACCTCG TTCACCCACA CAGGGCTGTC GTTCAACACC TGACTCCGCC 
CACTCAGTTG GTGGAAACTC ATCCCAAAGT AGCTCGCCTA GCTCCAGTGT GCCAAACTCT 
CCTGCCAGCT CTGGCCAGAT CAGACCCAGC TCCTTGCATG GTCTGGCCCC CAAGCTGCAG 
CGCCAGTACC GATCCCCCCG CCGGAAGTCT GCAGGCAACA TCCCTTTATC CCCTCTGGCC 
CGTACACCAT CGCCCACTCC ACAGAGCACT TCTCCCCAGC GTTCCCCTTC TCCCCTGCCA 
AGCCACGGGC TCATCACTTC TTCAATAGGC CAGTCTTTTC CTGTCAAACT GCACTCTTCA 
CCTCCATTAG TAAGACAGAT ATCTCGGCCA AAGAGTGCAG ACCCTCCTCG CTCTCCTCTG 
CTCAAACGAG TCCAGTCCGC AGAGAAACTC GCCGCCTCTC TTTCTTCTTC ATCCTCCTCT 
CCTTCTCCCT CTTCGGCTGC TGATAAAAAG CTTCCAGTTG GTGCCAGCCG CAAACACAGT 
CTTGATACCT CACATTCAGA GTTTAAAAAA GAGATGCTGC AGCGAGACCC TAGTCTACAG 
AGCCTGCAAG AGTCAGCCAG TGAGACTCTT ATGGGAGGAA GAGTGTCTCC TGCAGAAAAG 
GGAAGCCTGC AGAAAAGTTC AGTGCGTAAA CTGGGCAGGC AAGAAGGTCC CGAGTCTGGA 
ACAGGAACTC TGGGACTTGT TCCTGGAAAG AGTAAATTGA AAGACAAGCT GTCTGCCATA 
CGTGCTGAAC GCCGCGAATC TCTTCAAAAG CAGGATGCCA TCCATGAAGT GGATTCATCT 
GAAGATGAGA CAGACGAAGG GTCGGAAGAC AGCCAAGATG GGCGCAGAAC TTGCTATGCT 
CCTCCTAGCC ATGTCTTGAG GCCTACAACT GTAATGGCCT CTCCCCATAC CATCAGAATG 
GGGCCAGCGG CTCCACCAAC ACTGGGACTT TTTCCTTCAA CAGTTGCACC GCCATGCTCA 
CCTCAGCTCA TCAGAGGACT GCAGATACAA AACCAAAGTA CCACTCAATC TCAGACACAA 
ACTTCAACCT CACAACCAGC GCAAGCTGCA GTTAAACCCC CAGAACAGAA ACCAATAACC 
TCTGTTAAAG ACTCAAGTTC AACGATAATT GATGATTCGA AAAAACAGTC ACAGGCACAA 
GACATTCAGT CCAGACCTTC TCCACATCAA CCCAGTCCTC TGTCTAGTAC CTTTTCCACC 
CAAGGCAGCG CCTTTACCTC TGTTAGTAAA GACCCATTTG TCAAGCCCAC CACACCTCCA 
TTGGATCCCC GGAGGACCCC AAAAATGTCT GAGCCAAGGT CAAAAACCAG TGGTAGAACT 
GATTCTAGCA CTACTTCTGG AGTAGCCAGG GATACCCCAG TTGCGACTAC CCCAACAGGA 
GGAATCACTA AGGAGATGAA GGAGGCTGAC AATCGCAGAC AGGCTGCAGC TGCTGCTGCT 
GCCTCTGCCG CCGCTGCTAC CACCTCTGCA TCCACTGCCT CTGAAAGTGG AATGGATAAA 
GTTGTGTCTC AGCTCGCCAC TGTGGCCAAG AGTGTGCTTG GACCTGTCAA GCTCAATATC 
CCAGGTACTA AAGAGACCAT GGAACGAACA AAGGACCAGC GTACCCAGGC AGATGCCACC 
CACCCAAAGA TAAAAGAATG TCGCCTGGAG CACCCTGATT CTCCTGACCA ATTGAGTCCA 
TCTGCTGCTT CGGGCTCCCA GTCTCCACGA CTGACTGAAT CTCCATCTAA ACAAACATCA 
TCCAAATCTG AGCCAGCCTC AACTTCTCAG AGACCTTTAG AGGTCCCAGG GACTTTCAAA 
AGACAAACTT CTCCAATCCC TCAAGCTAGG GAAGAGCATC AGGATAGGTC AGGAACTCTG 
CAAAGTGGCA CCACATCAAG GCCTAGGTCT GAAGCACAAT CACAGACCCA GCAGGCAAAG 
CCCAGTTCCA CTACGTCACG AGATAAATCC AACAAGACAA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_009302494.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300769.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X1, mRNA.

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CloneID	ODa47560
Clone ID Related Accession (Same CDS sequence)	XM_009302494.3 , XM_009302494.2
Accession Version	XM_009302494.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302494.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAA CCGAGAGCAA AAACAAAAGG AAACTCTGCA CATCTGAGCA GCAGGACAGC 
AGCACTGAAG GAAAAGACGA GGAGCTGCCG GCGCCGCCTC CCCTGCTGTT CAGGAAGCTG 
AGTAACCCAG ACCTGTCTCC TGCCGCAGGA AAAACCAAAC TGCAGAGGCA GCTCAGTCAG 
GATGACAGCA GAGGCCGACG CAGCAGCATG GCTGGCATCC TCACTGGAAA GCAGCTTCTG 
CCTCTGTCCA GCAGTATGCA TGGAGGAGTG AGTCAGCTGG CCTGGCAGCA GCACACAGGG 
GAGCCCAATA ACTTGGTCCG CATGAGGAGT CAGTCCTTTG GCCAGTCTGC CCCGTCTCTC 
ACTGCGGGAC TGAAGGAGCT GAGTCTTCCT AGAAGAGGCA GTTTCTGTCG GACGAGCAAC 
CGAAAAAGTT TGATAGTGAC ATCCAGCACG TCTCCAACGC TGCCTCGACC CCATTCACCA 
CTGCACGGGC ATCCAGGAAG CAGCCCATTG GACAGCCCTC GCAATTTCTC CCCCAACACT 
GCAGCCCACT TCTCTTTTGT ACCAGCTCGA AGCCATGGAC ACAGGGCTGA CAGGACTGAC 
GGTCGTCGCT GGTCTCTCGC CTCTCTGCCG TCCTCTGGCT ATGGAACAAA CACCCCCAGT 
TCGACAGTCT CGTCATCATG TTCATCTCAG GAGAAGCTGC ACCAGTTGCC CTTCCAGCCC 
ACTGCAGACG AGCTGCATTT CCTCACCAAA CACTTCAGCT CGGAGAGCAT CACGGATGAG 
GATGGGAGAC GATCCCCAGC CATGCGTCCG CGCTCCCGCA GTCTCAGCCC TGGACGGTCA 
CCAATATCAT TTGACCATGA GATTGTCATG ATGAACCATG TCTACAAGGA GCGTTTTCCT 
AAGGCCACTG CTCAGATGGA GGTGCGTCTG GCCGATTTCA TATCCTCCTC AGCTCCAGAA 
AAAGTCATGC CTTTAGCTGA TGGCGTCCTG AGCTTCATCC ACCACCAGGT TATCGAACTG 
TCTCGAGATT GTCTGGACAA ATCTCGTGAG GGTCTCATCA CCTCCCGCTA CTTCTATGAG 
CTACAAGAGA ACCTTGAAAA GCTACACCAG GATGCTCATG AGCGTTCAGA AAGTGGGGAG 
GTGACATTTG TCACCCAGCT AGTTAAAAAG CTGATGATAA TCATCGCTCG GCCTGCCCGG 
CTGCTAGAAT GTTTGGAGTT TGACCCGGAG GAGTTCTACC ACCTGTTAGA GGCAGCTGAA 
GGTCATGCTA AAGAAGGTCA AGGCATCAAG TCTGACATCC CTCGATACAT CATCAGCCAG 
CTGGGCCTCA CCAGGGACCC CCTTGAGGAA ATGGCCCAGC TGAGCAGCTA TGACAGCGGT 
AACCCTGAAA CACCAGAGAC AGAGACAGAT TCCACAGATA GTCGAGGAGC AACCGTACAG 
CCACTCCAAC CTCAGCCTCA ACCGCAGCCC AAGACTAAAA CACCTCGCGA GGAAGACTTC 
GAGACCATTA AACTCATCAG TAATGGAGCC TATGGGGCTG TGTTTCTGGT GAGGCATAAA 
GAGACCCGTC AGCGATTTGC CATGAAGAAG ATCAACAAGC AGAACCTGAT CCTAAGAAAC 
CAGATCCAGC AGGCCTTTGT GGAAAGAGAC ATCTTGACAT TTGCAGAAAA CCCTTTTGTT 
GTCAGCATGT TCTGCTCCTT TGAGACAAGG AGACATCTTT GCATGGTTAT GGAGTATGTG 
GAGGGTGGGG ATTGTGCCAC TCTCCTGAAG CACATTGGAG CTCTGCCTGT GGATATGGCA 
CGCATGTACT TTGCTGAAAC CGTTCTTGCA TTGGAATACC TTCACAACTA TGGTATTGTG 
CACAGAGACC TCAAACCTGA CAATCTCCTG ATTACCTCAA TGGGACACGT CAAACTGACT 
GACTTTGGCC TGTCCAAGAT TGGTTTAATG AGCTTAACAA CTAATCTGTA TGAGGGCCAC 
ATTGAAAAAG ATACTCGAGA ATTCTTGGAC AAACAGGTGT GTGGTACTCC TGAATACATT 
GCTCCTGAGG TTATTCTGCG ACAGGGTTAT GGAAAACCAG TTGACTGGTG GGCCATGGGA 
GTCATCTTGT ATGAGTTTCT GGTGGGCTGT GCTCCTTTCT TTGGAGATAC ACCTGAAGAA 
CTGTTTGGAC AAGTCATCAG TGATGAGATA ATCTGGCCAG AAGGTGATGA AGCTTTGCCT 
CCGGATGCTC AAGATCTCAT CTCCAAACTT CTTCGGCAAA ACCCTCTAGA GCGTCTTGGG 
ACAGGGAGTG CGTTTGAGGT AAAACAGCAC CGCTTCTTTA CTGATCTGGA CTGGAACAGC 
CTCCTGAGGC AGAAGGCTGA GTTCATTCCT CAGCTGGAGT CGGAGGATGA CACTAGCTAC 
TTTGACACAC GCTCAGATCG TTATCATCAT GTGGACTCAG AGGAAGAAGA TGACACAAAT 
GATGACGACC ATTTGGAGAT CCGTCAGTTC TCCTCCTGTT CGCCACGATT CAGCAAGGTG 
TATAGTAGTA TGGAGCGTCT TTCCTTGCAT GAGGAGAAAC GCACCCCTCC TCCTACTAAG 
CGCAGTCTGA GTGAAGAGGG AGGAGAGCGG ATCGACAGTC TAAGTGGCCT AAAGTCCAGA 
GACCGAAGCT GGTTGGTGGG CTCACCTGAA ATTCTCAGAA AGCGTCTCTC TGTTTCCGAG 
TCTTCTCACA CCGAGAGTGA CTCGAGTCCA CCTCTGACTG TTCGTCGCCG CTGCTGCTCG 
GCCATCATTG AAATGCCTCG CTTCGCCATA TCCAGTGAAG AGGATAGCTG TGTGGGGAGC 
AGGAAGACCC CTGTGAGAGG CCCTCGAGGG GATGATCTGC CTCAGGCCAT ACCAGAGCTC 
CCTGTAGAGA GAGAGCTCAG GCTGGATGAA TCGCCCACCA CGCCAGGATC CACTTCCAGT 
CAAATCAGCC AGTCCACACT CACACCTGGT AGTTCTGGTG ATGTGTTAGA TCGTGCTTCT 
CGCTATAGTG CTGAAGTTTC TGAAAACTCC ACTCCAAGAG CCATCAGTGA TTTGGCGGCT 
CGAAGAGCTC GCCACAGACT GCTTTCAGGA GATACAGATA AACACACATC ACGTCCACTT 
AGCAAAGTCA TCAAATCAGC TTCAGCAACA ACCCTGTCAC TCATGATCCC TGCTGATCAC 
CATGGTGCTT CTCCACTGGC CAGTCCCATG TCGCCTCACT CTCTCTCTTC GAACCCCTCA 
TCACGTGACT CCTCGCCCAG CCGTGACCTT TCCCCAGCTG TCTGTAGTGT TAAACCTGCC 
ATAATCATCC ATCGAGCTGG AAAGAAATAT GGTTTTACTT TACGTGCAAT CCGGGTTTAC 
ATGGGAGACA CAGACATATA TACTGTCCAT CACATGGTCT GGCATGTGGA GGATGGAGGA 
CCTGCTCATG AGGCTGGACT GAGAGAGGGA GATCTCATCA CACATGTTAA CGGAGAGCCT 
GTCCATGGAC TTGTGCACAC AGAAGTTGTT GAGCTCATTC TTAAGAGTGG CAGTAAGGTG 
TCCATCTCTG CTACACCCTT TGAAAATACA TCAATTAAGG TTGGTCCTGC TCGCAAAACT 
AGCTACAAGT CCAAAATGGC CCGTCGCAAC AAAAGAACCA AAACTAGAGA AGGACAAGAC 
AGTAAGAAGA GGAATTCCCT CTTCCGTAAG ATCACCAAAC AGGCGACCCT TCTTCACACC 
AGTCGCAGCC TGTCCTCTCT GAATCGCTCG GTCTCTTCTG GTGAGAGCGT CCCAGGCTCT 
CCTACACACA TGTCACCTCG TTCACCCACA CAGGGCTGTC GTTCAACACC TGACTCCGCC 
CACTCAGTTG GTGGAAACTC ATCCCAAAGT AGCTCGCCTA GCTCCAGTGT GCCAAACTCT 
CCTGCCAGCT CTGGCCAGAT CAGACCCAGC TCCTTGCATG GTCTGGCCCC CAAGCTGCAG 
CGCCAGTACC GATCCCCCCG CCGGAAGTCT GCAGGCAACA TCCCTTTATC CCCTCTGGCC 
CGTACACCAT CGCCCACTCC ACAGAGCACT TCTCCCCAGC GTTCCCCTTC TCCCCTGCCA 
AGCCACGGGC TCATCACTTC TTCAATAGGC CAGTCTTTTC CTGTCAAACT GCACTCTTCA 
CCTCCATTAG TAAGACAGAT ATCTCGGCCA AAGAGTGCAG ACCCTCCTCG CTCTCCTCTG 
CTCAAACGAG TCCAGTCCGC AGAGAAACTC GCCGCCTCTC TTTCTTCTTC ATCCTCCTCT 
CCTTCTCCCT CTTCGGCTGC TGATAAAAAG CTTCCAGTTG GTGCCAGCCG CAAACACAGT 
CTTGATACCT CACATTCAGA GTTTAAAAAA GAGATGCTGC AGCGAGACCC TAGTCTACAG 
AGCCTGCAAG AGTCAGCCAG TGAGACTCTT ATGGGAGGAA GAGTGTCTCC TGCAGAAAAG 
GGAAGCCTGC AGAAAAGTTC AGTGCGTAAA CTGGGCAGGC AAGAAGGTCC CGAGTCTGGA 
ACAGGAACTC TGGGACTTGT TCCTGGAAAG AGTAAATTGA AAGACAAGCT GTCTGCCATA 
CGTGCTGAAC GCCGCGAATC TCTTCAAAAG CAGGATGCCA TCCATGAAGT GGATTCATCT 
GAAGATGAGA CAGACGAAGG GTCGGAAGAC AGCCAAGATG GGCGCAGAAC TTGCTATGCT 
CCTCCTAGCC ATGTCTTGAG GCCTACAACT GTAATGGCCT CTCCCCATAC CATCAGAATG 
GGGCCAGCGG CTCCACCAAC ACTGGGACTT TTTCCTTCAA CAGTTGCACC GCCATGCTCA 
CCTCAGCTCA TCAGAGGACT GCAGATACAA AACCAAAGTA CCACTCAATC TCAGACACAA 
ACTTCAACCT CACAACCAGC GCAAGCTGCA GTTAAACCCC CAGAACAGAA ACCAATAACC 
TCTGTTAAAG ACTCAAGTTC AACGATAATT GATGATTCGA AAAAACAGTC ACAGGCACAA 
GACATTCAGT CCAGACCTTC TCCACATCAA CCCAGTCCTC TGTCTAGTAC CTTTTCCACC 
CAAGGCAGCG CCTTTACCTC TGTTAGTAAA GACCCATTTG TCAAGCCCAC CACACCTCCA 
TTGGATCCCC GGAGGACCCC AAAAATGTCT GAGCCAAGGT CAAAAACCAG TGGTAGAACT 
GATTCTAGCA CTACTTCTGG AGTAGCCAGG GATACCCCAG TTGCGACTAC CCCAACAGGA 
GGAATCACTA AGGAGATGAA GGAGGCTGAC AATCGCAGAC AGGCTGCAGC TGCTGCTGCT 
GCCTCTGCCG CCGCTGCTAC CACCTCTGCA TCCACTGCCT CTGAAAGTGG AATGGATAAA 
GTTGTGTCTC AGCTCGCCAC TGTGGCCAAG AGTGTGCTTG GACCTGTCAA GCTCAATATC 
CCAGGTACTA AAGAGACCAT GGAACGAACA AAGGACCAGC GTACCCAGGC AGATGCCACC 
CACCCAAAGA TAAAAGAATG TCGCCTGGAG CACCCTGATT CTCCTGACCA ATTGAGTCCA 
TCTGCTGCTT CGGGCTCCCA GTCTCCACGA CTGACTGAAT CTCCATCTAA ACAAACATCA 
TCCAAATCTG AGCCAGCCTC AACTTCTCAG AGACCTTTAG AGGTCCCAGG GACTTTCAAA 
AGACAAACTT CTCCAATCCC TCAAGCTAGG GAAGAGCATC AGGATAGGTC AGGAACTCTG 
CAAAGTGGCA CCACATCAAG GCCTAGGTCT GAAGCACAAT CACAGACCCA GCAGGCAAAG 
CCCAGTTCCA CTACGTCACG AGATAAATCC AACAAGACAA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300769.1
CDS	1077..6881
Translation	MKKTESKNKRKLCTSEQQDSSTEGKDEELPAPPPLLFRKLSNPDLSPAAGKTKLQRQLSQDDSRGRRSSMAGILTGKQLLPLSSSMHGGVSQLAWQQHTGEPNNLVRMRSQSFGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYAPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPCSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_009302494.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302494.2

ORF Insert Sequence:

ATGAAGAAAA CCGAGAGCAA AAACAAAAGG AAACTCTGCA CATCTGAGCA GCAGGACAGC 
AGCACTGAAG GAAAAGACGA GGAGCTGCCG GCGCCGCCTC CCCTGCTGTT CAGGAAGCTG 
AGTAACCCAG ACCTGTCTCC TGCCGCAGGA AAAACCAAAC TGCAGAGGCA GCTCAGTCAG 
GATGACAGCA GAGGCCGACG CAGCAGCATG GCTGGCATCC TCACTGGAAA GCAGCTTCTG 
CCTCTGTCCA GCAGTATGCA TGGAGGAGTG AGTCAGCTGG CCTGGCAGCA GCACACAGGG 
GAGCCCAATA ACTTGGTCCG CATGAGGAGT CAGTCCTTTG GCCAGTCTGC CCCGTCTCTC 
ACTGCGGGAC TGAAGGAGCT GAGTCTTCCT AGAAGAGGCA GTTTCTGTCG GACGAGCAAC 
CGAAAAAGTT TGATAGTGAC ATCCAGCACG TCTCCAACGC TGCCTCGACC CCATTCACCA 
CTGCACGGGC ATCCAGGAAG CAGCCCATTG GACAGCCCTC GCAATTTCTC CCCCAACACT 
GCAGCCCACT TCTCTTTTGT ACCAGCTCGA AGCCATGGAC ACAGGGCTGA CAGGACTGAC 
GGTCGTCGCT GGTCTCTCGC CTCTCTGCCG TCCTCTGGCT ATGGAACAAA CACCCCCAGT 
TCGACAGTCT CGTCATCATG TTCATCTCAG GAGAAGCTGC ACCAGTTGCC CTTCCAGCCC 
ACTGCAGACG AGCTGCATTT CCTCACCAAA CACTTCAGCT CGGAGAGCAT CACGGATGAG 
GATGGGAGAC GATCCCCAGC CATGCGTCCG CGCTCCCGCA GTCTCAGCCC TGGACGGTCA 
CCAATATCAT TTGACCATGA GATTGTCATG ATGAACCATG TCTACAAGGA GCGTTTTCCT 
AAGGCCACTG CTCAGATGGA GGTGCGTCTG GCCGATTTCA TATCCTCCTC AGCTCCAGAA 
AAAGTCATGC CTTTAGCTGA TGGCGTCCTG AGCTTCATCC ACCACCAGGT TATCGAACTG 
TCTCGAGATT GTCTGGACAA ATCTCGTGAG GGTCTCATCA CCTCCCGCTA CTTCTATGAG 
CTACAAGAGA ACCTTGAAAA GCTACACCAG GATGCTCATG AGCGTTCAGA AAGTGGGGAG 
GTGACATTTG TCACCCAGCT AGTTAAAAAG CTGATGATAA TCATCGCTCG GCCTGCCCGG 
CTGCTAGAAT GTTTGGAGTT TGACCCGGAG GAGTTCTACC ACCTGTTAGA GGCAGCTGAA 
GGTCATGCTA AAGAAGGTCA AGGCATCAAG TCTGACATCC CTCGATACAT CATCAGCCAG 
CTGGGCCTCA CCAGGGACCC CCTTGAGGAA ATGGCCCAGC TGAGCAGCTA TGACAGCGGT 
AACCCTGAAA CACCAGAGAC AGAGACAGAT TCCACAGATA GTCGAGGAGC AACCGTACAG 
CCACTCCAAC CTCAGCCTCA ACCGCAGCCC AAGACTAAAA CACCTCGCGA GGAAGACTTC 
GAGACCATTA AACTCATCAG TAATGGAGCC TATGGGGCTG TGTTTCTGGT GAGGCATAAA 
GAGACCCGTC AGCGATTTGC CATGAAGAAG ATCAACAAGC AGAACCTGAT CCTAAGAAAC 
CAGATCCAGC AGGCCTTTGT GGAAAGAGAC ATCTTGACAT TTGCAGAAAA CCCTTTTGTT 
GTCAGCATGT TCTGCTCCTT TGAGACAAGG AGACATCTTT GCATGGTTAT GGAGTATGTG 
GAGGGTGGGG ATTGTGCCAC TCTCCTGAAG CACATTGGAG CTCTGCCTGT GGATATGGCA 
CGCATGTACT TTGCTGAAAC CGTTCTTGCA TTGGAATACC TTCACAACTA TGGTATTGTG 
CACAGAGACC TCAAACCTGA CAATCTCCTG ATTACCTCAA TGGGACACGT CAAACTGACT 
GACTTTGGCC TGTCCAAGAT TGGTTTAATG AGCTTAACAA CTAATCTGTA TGAGGGCCAC 
ATTGAAAAAG ATACTCGAGA ATTCTTGGAC AAACAGGTGT GTGGTACTCC TGAATACATT 
GCTCCTGAGG TTATTCTGCG ACAGGGTTAT GGAAAACCAG TTGACTGGTG GGCCATGGGA 
GTCATCTTGT ATGAGTTTCT GGTGGGCTGT GCTCCTTTCT TTGGAGATAC ACCTGAAGAA 
CTGTTTGGAC AAGTCATCAG TGATGAGATA ATCTGGCCAG AAGGTGATGA AGCTTTGCCT 
CCGGATGCTC AAGATCTCAT CTCCAAACTT CTTCGGCAAA ACCCTCTAGA GCGTCTTGGG 
ACAGGGAGTG CGTTTGAGGT AAAACAGCAC CGCTTCTTTA CTGATCTGGA CTGGAACAGC 
CTCCTGAGGC AGAAGGCTGA GTTCATTCCT CAGCTGGAGT CGGAGGATGA CACTAGCTAC 
TTTGACACAC GCTCAGATCG TTATCATCAT GTGGACTCAG AGGAAGAAGA TGACACAAAT 
GATGACGACC ATTTGGAGAT CCGTCAGTTC TCCTCCTGTT CGCCACGATT CAGCAAGGTG 
TATAGTAGTA TGGAGCGTCT TTCCTTGCAT GAGGAGAAAC GCACCCCTCC TCCTACTAAG 
CGCAGTCTGA GTGAAGAGGG AGGAGAGCGG ATCGACAGTC TAAGTGGCCT AAAGTCCAGA 
GACCGAAGCT GGTTGGTGGG CTCACCTGAA ATTCTCAGAA AGCGTCTCTC TGTTTCCGAG 
TCTTCTCACA CCGAGAGTGA CTCGAGTCCA CCTCTGACTG TTCGTCGCCG CTGCTGCTCG 
GCCATCATTG AAATGCCTCG CTTCGCCATA TCCAGTGAAG AGGATAGCTG TGTGGGGAGC 
AGGAAGACCC CTGTGAGAGG CCCTCGAGGG GATGATCTGC CTCAGGCCAT ACCAGAGCTC 
CCTGTAGAGA GAGAGCTCAG GCTGGATGAA TCGCCCACCA CGCCAGGATC CACTTCCAGT 
CAAATCAGCC AGTCCACACT CACACCTGGT AGTTCTGGTG ATGTGTTAGA TCGTGCTTCT 
CGCTATAGTG CTGAAGTTTC TGAAAACTCC ACTCCAAGAG CCATCAGTGA TTTGGCGGCT 
CGAAGAGCTC GCCACAGACT GCTTTCAGGA GATACAGATA AACACACATC ACGTCCACTT 
AGCAAAGTCA TCAAATCAGC TTCAGCAACA ACCCTGTCAC TCATGATCCC TGCTGATCAC 
CATGGTGCTT CTCCACTGGC CAGTCCCATG TCGCCTCACT CTCTCTCTTC GAACCCCTCA 
TCACGTGACT CCTCGCCCAG CCGTGACCTT TCCCCAGCTG TCTGTAGTGT TAAACCTGCC 
ATAATCATCC ATCGAGCTGG AAAGAAATAT GGTTTTACTT TACGTGCAAT CCGGGTTTAC 
ATGGGAGACA CAGACATATA TACTGTCCAT CACATGGTCT GGCATGTGGA GGATGGAGGA 
CCTGCTCATG AGGCTGGACT GAGAGAGGGA GATCTCATCA CACATGTTAA CGGAGAGCCT 
GTCCATGGAC TTGTGCACAC AGAAGTTGTT GAGCTCATTC TTAAGAGTGG CAGTAAGGTG 
TCCATCTCTG CTACACCCTT TGAAAATACA TCAATTAAGG TTGGTCCTGC TCGCAAAACT 
AGCTACAAGT CCAAAATGGC CCGTCGCAAC AAAAGAACCA AAACTAGAGA AGGACAAGAC 
AGTAAGAAGA GGAATTCCCT CTTCCGTAAG ATCACCAAAC AGGCGACCCT TCTTCACACC 
AGTCGCAGCC TGTCCTCTCT GAATCGCTCG GTCTCTTCTG GTGAGAGCGT CCCAGGCTCT 
CCTACACACA TGTCACCTCG TTCACCCACA CAGGGCTGTC GTTCAACACC TGACTCCGCC 
CACTCAGTTG GTGGAAACTC ATCCCAAAGT AGCTCGCCTA GCTCCAGTGT GCCAAACTCT 
CCTGCCAGCT CTGGCCAGAT CAGACCCAGC TCCTTGCATG GTCTGGCCCC CAAGCTGCAG 
CGCCAGTACC GATCCCCCCG CCGGAAGTCT GCAGGCAACA TCCCTTTATC CCCTCTGGCC 
CGTACACCAT CGCCCACTCC ACAGAGCACT TCTCCCCAGC GTTCCCCTTC TCCCCTGCCA 
AGCCACGGGC TCATCACTTC TTCAATAGGC CAGTCTTTTC CTGTCAAACT GCACTCTTCA 
CCTCCATTAG TAAGACAGAT ATCTCGGCCA AAGAGTGCAG ACCCTCCTCG CTCTCCTCTG 
CTCAAACGAG TCCAGTCCGC AGAGAAACTC GCCGCCTCTC TTTCTTCTTC ATCCTCCTCT 
CCTTCTCCCT CTTCGGCTGC TGATAAAAAG CTTCCAGTTG GTGCCAGCCG CAAACACAGT 
CTTGATACCT CACATTCAGA GTTTAAAAAA GAGATGCTGC AGCGAGACCC TAGTCTACAG 
AGCCTGCAAG AGTCAGCCAG TGAGACTCTT ATGGGAGGAA GAGTGTCTCC TGCAGAAAAG 
GGAAGCCTGC AGAAAAGTTC AGTGCGTAAA CTGGGCAGGC AAGAAGGTCC CGAGTCTGGA 
ACAGGAACTC TGGGACTTGT TCCTGGAAAG AGTAAATTGA AAGACAAGCT GTCTGCCATA 
CGTGCTGAAC GCCGCGAATC TCTTCAAAAG CAGGATGCCA TCCATGAAGT GGATTCATCT 
GAAGATGAGA CAGACGAAGG GTCGGAAGAC AGCCAAGATG GGCGCAGAAC TTGCTATGCT 
CCTCCTAGCC ATGTCTTGAG GCCTACAACT GTAATGGCCT CTCCCCATAC CATCAGAATG 
GGGCCAGCGG CTCCACCAAC ACTGGGACTT TTTCCTTCAA CAGTTGCACC GCCATGCTCA 
CCTCAGCTCA TCAGAGGACT GCAGATACAA AACCAAAGTA CCACTCAATC TCAGACACAA 
ACTTCAACCT CACAACCAGC GCAAGCTGCA GTTAAACCCC CAGAACAGAA ACCAATAACC 
TCTGTTAAAG ACTCAAGTTC AACGATAATT GATGATTCGA AAAAACAGTC ACAGGCACAA 
GACATTCAGT CCAGACCTTC TCCACATCAA CCCAGTCCTC TGTCTAGTAC CTTTTCCACC 
CAAGGCAGCG CCTTTACCTC TGTTAGTAAA GACCCATTTG TCAAGCCCAC CACACCTCCA 
TTGGATCCCC GGAGGACCCC AAAAATGTCT GAGCCAAGGT CAAAAACCAG TGGTAGAACT 
GATTCTAGCA CTACTTCTGG AGTAGCCAGG GATACCCCAG TTGCGACTAC CCCAACAGGA 
GGAATCACTA AGGAGATGAA GGAGGCTGAC AATCGCAGAC AGGCTGCAGC TGCTGCTGCT 
GCCTCTGCCG CCGCTGCTAC CACCTCTGCA TCCACTGCCT CTGAAAGTGG AATGGATAAA 
GTTGTGTCTC AGCTCGCCAC TGTGGCCAAG AGTGTGCTTG GACCTGTCAA GCTCAATATC 
CCAGGTACTA AAGAGACCAT GGAACGAACA AAGGACCAGC GTACCCAGGC AGATGCCACC 
CACCCAAAGA TAAAAGAATG TCGCCTGGAG CACCCTGATT CTCCTGACCA ATTGAGTCCA 
TCTGCTGCTT CGGGCTCCCA GTCTCCACGA CTGACTGAAT CTCCATCTAA ACAAACATCA 
TCCAAATCTG AGCCAGCCTC AACTTCTCAG AGACCTTTAG AGGTCCCAGG GACTTTCAAA 
AGACAAACTT CTCCAATCCC TCAAGCTAGG GAAGAGCATC AGGATAGGTC AGGAACTCTG 
CAAAGTGGCA CCACATCAAG GCCTAGGTCT GAAGCACAAT CACAGACCCA GCAGGCAAAG 
CCCAGTTCCA CTACGTCACG AGATAAATCC AACAAGACAA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_009302495.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300770.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X2, mRNA.

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CloneID	ODa47561
Clone ID Related Accession (Same CDS sequence)	XM_009302495.2
Accession Version	XM_009302495.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAA CCGAGAGCAA AAACAAAAGG AAACTCTGCA CATCTGAGCA GCAGGACAGC 
AGCACTGAAG GAAAAGACGA GGAGCTGCCG GCGCCGCCTC CCCTGCTGTT CAGGAAGCTG 
AGTAACCCAG ACCTGTCTCC TGCCGCAGGA AAAACCAAAC TGCAGAGGCA GCTCAGTCAG 
GATGACAGCA GAGGCCGACG CAGCAGCATG GCTGGCATCC TCACTGGAAA GCAGCTTCTG 
CCTCTGTCCA GCAGTATGCA TGGAGGAGTG AGTCAGCTGG CCTGGCAGCA GCACACAGGG 
GAGCCCAATA ACTTGGTCCG CATGAGGAGT CAGTCCTTTG GCCAGTCTGC CCCGTCTCTC 
ACTGCGGGAC TGAAGGAGCT GAGTCTTCCT AGAAGAGGCA GTTTCTGTCG GACGAGCAAC 
CGAAAAAGTT TGATAGTGAC ATCCAGCACG TCTCCAACGC TGCCTCGACC CCATTCACCA 
CTGCACGGGC ATCCAGGAAG CAGCCCATTG GACAGCCCTC GCAATTTCTC CCCCAACACT 
GCAGCCCACT TCTCTTTTGT ACCAGCTCGA AGCCATGGAC ACAGGGCTGA CAGGACTGAC 
GGTCGTCGCT GGTCTCTCGC CTCTCTGCCG TCCTCTGGCT ATGGAACAAA CACCCCCAGT 
TCGACAGTCT CGGAGAAGCT GCACCAGTTG CCCTTCCAGC CCACTGCAGA CGAGCTGCAT 
TTCCTCACCA AACACTTCAG CTCGGAGAGC ATCACGGATG AGGATGGGAG ACGATCCCCA 
GCCATGCGTC CGCGCTCCCG CAGTCTCAGC CCTGGACGGT CACCAATATC ATTTGACCAT 
GAGATTGTCA TGATGAACCA TGTCTACAAG GAGCGTTTTC CTAAGGCCAC TGCTCAGATG 
GAGGTGCGTC TGGCCGATTT CATATCCTCC TCAGCTCCAG AAAAAGTCAT GCCTTTAGCT 
GATGGCGTCC TGAGCTTCAT CCACCACCAG GTTATCGAAC TGTCTCGAGA TTGTCTGGAC 
AAATCTCGTG AGGGTCTCAT CACCTCCCGC TACTTCTATG AGCTACAAGA GAACCTTGAA 
AAGCTACACC AGGATGCTCA TGAGCGTTCA GAAAGTGGGG AGGTGACATT TGTCACCCAG 
CTAGTTAAAA AGCTGATGAT AATCATCGCT CGGCCTGCCC GGCTGCTAGA ATGTTTGGAG 
TTTGACCCGG AGGAGTTCTA CCACCTGTTA GAGGCAGCTG AAGGTCATGC TAAAGAAGGT 
CAAGGCATCA AGTCTGACAT CCCTCGATAC ATCATCAGCC AGCTGGGCCT CACCAGGGAC 
CCCCTTGAGG AAATGGCCCA GCTGAGCAGC TATGACAGCG GTAACCCTGA AACACCAGAG 
ACAGAGACAG ATTCCACAGA TAGTCGAGGA GCAACCGTAC AGCCACTCCA ACCTCAGCCT 
CAACCGCAGC CCAAGACTAA AACACCTCGC GAGGAAGACT TCGAGACCAT TAAACTCATC 
AGTAATGGAG CCTATGGGGC TGTGTTTCTG GTGAGGCATA AAGAGACCCG TCAGCGATTT 
GCCATGAAGA AGATCAACAA GCAGAACCTG ATCCTAAGAA ACCAGATCCA GCAGGCCTTT 
GTGGAAAGAG ACATCTTGAC ATTTGCAGAA AACCCTTTTG TTGTCAGCAT GTTCTGCTCC 
TTTGAGACAA GGAGACATCT TTGCATGGTT ATGGAGTATG TGGAGGGTGG GGATTGTGCC 
ACTCTCCTGA AGCACATTGG AGCTCTGCCT GTGGATATGG CACGCATGTA CTTTGCTGAA 
ACCGTTCTTG CATTGGAATA CCTTCACAAC TATGGTATTG TGCACAGAGA CCTCAAACCT 
GACAATCTCC TGATTACCTC AATGGGACAC GTCAAACTGA CTGACTTTGG CCTGTCCAAG 
ATTGGTTTAA TGAGCTTAAC AACTAATCTG TATGAGGGCC ACATTGAAAA AGATACTCGA 
GAATTCTTGG ACAAACAGGT GTGTGGTACT CCTGAATACA TTGCTCCTGA GGTTATTCTG 
CGACAGGGTT ATGGAAAACC AGTTGACTGG TGGGCCATGG GAGTCATCTT GTATGAGTTT 
CTGGTGGGCT GTGCTCCTTT CTTTGGAGAT ACACCTGAAG AACTGTTTGG ACAAGTCATC 
AGTGATGAGA TAATCTGGCC AGAAGGTGAT GAAGCTTTGC CTCCGGATGC TCAAGATCTC 
ATCTCCAAAC TTCTTCGGCA AAACCCTCTA GAGCGTCTTG GGACAGGGAG TGCGTTTGAG 
GTAAAACAGC ACCGCTTCTT TACTGATCTG GACTGGAACA GCCTCCTGAG GCAGAAGGCT 
GAGTTCATTC CTCAGCTGGA GTCGGAGGAT GACACTAGCT ACTTTGACAC ACGCTCAGAT 
CGTTATCATC ATGTGGACTC AGAGGAAGAA GATGACACAA ATGATGACGA CCATTTGGAG 
ATCCGTCAGT TCTCCTCCTG TTCGCCACGA TTCAGCAAGG TGTATAGTAG TATGGAGCGT 
CTTTCCTTGC ATGAGGAGAA ACGCACCCCT CCTCCTACTA AGCGCAGTCT GAGTGAAGAG 
GGAGGAGAGC GGATCGACAG TCTAAGTGGC CTAAAGTCCA GAGACCGAAG CTGGTTGGTG 
GGCTCACCTG AAATTCTCAG AAAGCGTCTC TCTGTTTCCG AGTCTTCTCA CACCGAGAGT 
GACTCGAGTC CACCTCTGAC TGTTCGTCGC CGCTGCTGCT CGGCCATCAT TGAAATGCCT 
CGCTTCGCCA TATCCAGTGA AGAGGATAGC TGTGTGGGGA GCAGGAAGAC CCCTGTGAGA 
GGCCCTCGAG GGGATGATCT GCCTCAGGCC ATACCAGAGC TCCCTGTAGA GAGAGAGCTC 
AGGCTGGATG AATCGCCCAC CACGCCAGGA TCCACTTCCA GTCAAATCAG CCAGTCCACA 
CTCACACCTG GTAGTTCTGG TGATGTGTTA GATCGTGCTT CTCGCTATAG TGCTGAAGTT 
TCTGAAAACT CCACTCCAAG AGCCATCAGT GATTTGGCGG CTCGAAGAGC TCGCCACAGA 
CTGCTTTCAG GAGATACAGA TAAACACACA TCACGTCCAC TTAGCAAAGT CATCAAATCA 
GCTTCAGCAA CAACCCTGTC ACTCATGATC CCTGCTGATC ACCATGGTGC TTCTCCACTG 
GCCAGTCCCA TGTCGCCTCA CTCTCTCTCT TCGAACCCCT CATCACGTGA CTCCTCGCCC 
AGCCGTGACC TTTCCCCAGC TGTCTGTAGT GTTAAACCTG CCATAATCAT CCATCGAGCT 
GGAAAGAAAT ATGGTTTTAC TTTACGTGCA ATCCGGGTTT ACATGGGAGA CACAGACATA 
TATACTGTCC ATCACATGGT CTGGCATGTG GAGGATGGAG GACCTGCTCA TGAGGCTGGA 
CTGAGAGAGG GAGATCTCAT CACACATGTT AACGGAGAGC CTGTCCATGG ACTTGTGCAC 
ACAGAAGTTG TTGAGCTCAT TCTTAAGAGT GGCAGTAAGG TGTCCATCTC TGCTACACCC 
TTTGAAAATA CATCAATTAA GGTTGGTCCT GCTCGCAAAA CTAGCTACAA GTCCAAAATG 
GCCCGTCGCA ACAAAAGAAC CAAAACTAGA GAAGGACAAG ACAGTAAGAA GAGGAATTCC 
CTCTTCCGTA AGATCACCAA ACAGGCGACC CTTCTTCACA CCAGTCGCAG CCTGTCCTCT 
CTGAATCGCT CGGTCTCTTC TGGTGAGAGC GTCCCAGGCT CTCCTACACA CATGTCACCT 
CGTTCACCCA CACAGGGCTG TCGTTCAACA CCTGACTCCG CCCACTCAGT TGGTGGAAAC 
TCATCCCAAA GTAGCTCGCC TAGCTCCAGT GTGCCAAACT CTCCTGCCAG CTCTGGCCAG 
ATCAGACCCA GCTCCTTGCA TGGTCTGGCC CCCAAGCTGC AGCGCCAGTA CCGATCCCCC 
CGCCGGAAGT CTGCAGGCAA CATCCCTTTA TCCCCTCTGG CCCGTACACC ATCGCCCACT 
CCACAGAGCA CTTCTCCCCA GCGTTCCCCT TCTCCCCTGC CAAGCCACGG GCTCATCACT 
TCTTCAATAG GCCAGTCTTT TCCTGTCAAA CTGCACTCTT CACCTCCATT AGTAAGACAG 
ATATCTCGGC CAAAGAGTGC AGACCCTCCT CGCTCTCCTC TGCTCAAACG AGTCCAGTCC 
GCAGAGAAAC TCGCCGCCTC TCTTTCTTCT TCATCCTCCT CTCCTTCTCC CTCTTCGGCT 
GCTGATAAAA AGCTTCCAGT TGGTGCCAGC CGCAAACACA GTCTTGATAC CTCACATTCA 
GAGTTTAAAA AAGAGATGCT GCAGCGAGAC CCTAGTCTAC AGAGCCTGCA AGAGTCAGCC 
AGTGAGACTC TTATGGGAGG AAGAGTGTCT CCTGCAGAAA AGGGAAGCCT GCAGAAAAGT 
TCAGTGCGTA AACTGGGCAG GCAAGAAGGT CCCGAGTCTG GAACAGGAAC TCTGGGACTT 
GTTCCTGGAA AGAGTAAATT GAAAGACAAG CTGTCTGCCA TACGTGCTGA ACGCCGCGAA 
TCTCTTCAAA AGCAGGATGC CATCCATGAA GTGGATTCAT CTGAAGATGA GACAGACGAA 
GGGTCGGAAG ACAGCCAAGA TGGGCGCAGA ACTTGCTATG CTCCTCCTAG CCATGTCTTG 
AGGCCTACAA CTGTAATGGC CTCTCCCCAT ACCATCAGAA TGGGGCCAGC GGCTCCACCA 
ACACTGGGAC TTTTTCCTTC AACAGTTGCA CCGCCATGCT CACCTCAGCT CATCAGAGGA 
CTGCAGATAC AAAACCAAAG TACCACTCAA TCTCAGACAC AAACTTCAAC CTCACAACCA 
GCGCAAGCTG CAGTTAAACC CCCAGAACAG AAACCAATAA CCTCTGTTAA AGACTCAAGT 
TCAACGATAA TTGATGATTC GAAAAAACAG TCACAGGCAC AAGACATTCA GTCCAGACCT 
TCTCCACATC AACCCAGTCC TCTGTCTAGT ACCTTTTCCA CCCAAGGCAG CGCCTTTACC 
TCTGTTAGTA AAGACCCATT TGTCAAGCCC ACCACACCTC CATTGGATCC CCGGAGGACC 
CCAAAAATGT CTGAGCCAAG GTCAAAAACC AGTGGTAGAA CTGATTCTAG CACTACTTCT 
GGAGTAGCCA GGGATACCCC AGTTGCGACT ACCCCAACAG GAGGAATCAC TAAGGAGATG 
AAGGAGGCTG ACAATCGCAG ACAGGCTGCA GCTGCTGCTG CTGCCTCTGC CGCCGCTGCT 
ACCACCTCTG CATCCACTGC CTCTGAAAGT GGAATGGATA AAGTTGTGTC TCAGCTCGCC 
ACTGTGGCCA AGAGTGTGCT TGGACCTGTC AAGCTCAATA TCCCAGGTAC TAAAGAGACC 
ATGGAACGAA CAAAGGACCA GCGTACCCAG GCAGATGCCA CCCACCCAAA GATAAAAGAA 
TGTCGCCTGG AGCACCCTGA TTCTCCTGAC CAATTGAGTC CATCTGCTGC TTCGGGCTCC 
CAGTCTCCAC GACTGACTGA ATCTCCATCT AAACAAACAT CATCCAAATC TGAGCCAGCC 
TCAACTTCTC AGAGACCTTT AGAGGTCCCA GGGACTTTCA AAAGACAAAC TTCTCCAATC 
CCTCAAGCTA GGGAAGAGCA TCAGGATAGG TCAGGAACTC TGCAAAGTGG CACCACATCA 
AGGCCTAGGT CTGAAGCACA ATCACAGACC CAGCAGGCAA AGCCCAGTTC CACTACGTCA 
CGAGATAAAT CCAACAAGAC AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300770.1
CDS	1078..6864
Translation	MKKTESKNKRKLCTSEQQDSSTEGKDEELPAPPPLLFRKLSNPDLSPAAGKTKLQRQLSQDDSRGRRSSMAGILTGKQLLPLSSSMHGGVSQLAWQQHTGEPNNLVRMRSQSFGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYAPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPCSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_009302495.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302495.2

ORF Insert Sequence:

ATGAAGAAAA CCGAGAGCAA AAACAAAAGG AAACTCTGCA CATCTGAGCA GCAGGACAGC 
AGCACTGAAG GAAAAGACGA GGAGCTGCCG GCGCCGCCTC CCCTGCTGTT CAGGAAGCTG 
AGTAACCCAG ACCTGTCTCC TGCCGCAGGA AAAACCAAAC TGCAGAGGCA GCTCAGTCAG 
GATGACAGCA GAGGCCGACG CAGCAGCATG GCTGGCATCC TCACTGGAAA GCAGCTTCTG 
CCTCTGTCCA GCAGTATGCA TGGAGGAGTG AGTCAGCTGG CCTGGCAGCA GCACACAGGG 
GAGCCCAATA ACTTGGTCCG CATGAGGAGT CAGTCCTTTG GCCAGTCTGC CCCGTCTCTC 
ACTGCGGGAC TGAAGGAGCT GAGTCTTCCT AGAAGAGGCA GTTTCTGTCG GACGAGCAAC 
CGAAAAAGTT TGATAGTGAC ATCCAGCACG TCTCCAACGC TGCCTCGACC CCATTCACCA 
CTGCACGGGC ATCCAGGAAG CAGCCCATTG GACAGCCCTC GCAATTTCTC CCCCAACACT 
GCAGCCCACT TCTCTTTTGT ACCAGCTCGA AGCCATGGAC ACAGGGCTGA CAGGACTGAC 
GGTCGTCGCT GGTCTCTCGC CTCTCTGCCG TCCTCTGGCT ATGGAACAAA CACCCCCAGT 
TCGACAGTCT CGGAGAAGCT GCACCAGTTG CCCTTCCAGC CCACTGCAGA CGAGCTGCAT 
TTCCTCACCA AACACTTCAG CTCGGAGAGC ATCACGGATG AGGATGGGAG ACGATCCCCA 
GCCATGCGTC CGCGCTCCCG CAGTCTCAGC CCTGGACGGT CACCAATATC ATTTGACCAT 
GAGATTGTCA TGATGAACCA TGTCTACAAG GAGCGTTTTC CTAAGGCCAC TGCTCAGATG 
GAGGTGCGTC TGGCCGATTT CATATCCTCC TCAGCTCCAG AAAAAGTCAT GCCTTTAGCT 
GATGGCGTCC TGAGCTTCAT CCACCACCAG GTTATCGAAC TGTCTCGAGA TTGTCTGGAC 
AAATCTCGTG AGGGTCTCAT CACCTCCCGC TACTTCTATG AGCTACAAGA GAACCTTGAA 
AAGCTACACC AGGATGCTCA TGAGCGTTCA GAAAGTGGGG AGGTGACATT TGTCACCCAG 
CTAGTTAAAA AGCTGATGAT AATCATCGCT CGGCCTGCCC GGCTGCTAGA ATGTTTGGAG 
TTTGACCCGG AGGAGTTCTA CCACCTGTTA GAGGCAGCTG AAGGTCATGC TAAAGAAGGT 
CAAGGCATCA AGTCTGACAT CCCTCGATAC ATCATCAGCC AGCTGGGCCT CACCAGGGAC 
CCCCTTGAGG AAATGGCCCA GCTGAGCAGC TATGACAGCG GTAACCCTGA AACACCAGAG 
ACAGAGACAG ATTCCACAGA TAGTCGAGGA GCAACCGTAC AGCCACTCCA ACCTCAGCCT 
CAACCGCAGC CCAAGACTAA AACACCTCGC GAGGAAGACT TCGAGACCAT TAAACTCATC 
AGTAATGGAG CCTATGGGGC TGTGTTTCTG GTGAGGCATA AAGAGACCCG TCAGCGATTT 
GCCATGAAGA AGATCAACAA GCAGAACCTG ATCCTAAGAA ACCAGATCCA GCAGGCCTTT 
GTGGAAAGAG ACATCTTGAC ATTTGCAGAA AACCCTTTTG TTGTCAGCAT GTTCTGCTCC 
TTTGAGACAA GGAGACATCT TTGCATGGTT ATGGAGTATG TGGAGGGTGG GGATTGTGCC 
ACTCTCCTGA AGCACATTGG AGCTCTGCCT GTGGATATGG CACGCATGTA CTTTGCTGAA 
ACCGTTCTTG CATTGGAATA CCTTCACAAC TATGGTATTG TGCACAGAGA CCTCAAACCT 
GACAATCTCC TGATTACCTC AATGGGACAC GTCAAACTGA CTGACTTTGG CCTGTCCAAG 
ATTGGTTTAA TGAGCTTAAC AACTAATCTG TATGAGGGCC ACATTGAAAA AGATACTCGA 
GAATTCTTGG ACAAACAGGT GTGTGGTACT CCTGAATACA TTGCTCCTGA GGTTATTCTG 
CGACAGGGTT ATGGAAAACC AGTTGACTGG TGGGCCATGG GAGTCATCTT GTATGAGTTT 
CTGGTGGGCT GTGCTCCTTT CTTTGGAGAT ACACCTGAAG AACTGTTTGG ACAAGTCATC 
AGTGATGAGA TAATCTGGCC AGAAGGTGAT GAAGCTTTGC CTCCGGATGC TCAAGATCTC 
ATCTCCAAAC TTCTTCGGCA AAACCCTCTA GAGCGTCTTG GGACAGGGAG TGCGTTTGAG 
GTAAAACAGC ACCGCTTCTT TACTGATCTG GACTGGAACA GCCTCCTGAG GCAGAAGGCT 
GAGTTCATTC CTCAGCTGGA GTCGGAGGAT GACACTAGCT ACTTTGACAC ACGCTCAGAT 
CGTTATCATC ATGTGGACTC AGAGGAAGAA GATGACACAA ATGATGACGA CCATTTGGAG 
ATCCGTCAGT TCTCCTCCTG TTCGCCACGA TTCAGCAAGG TGTATAGTAG TATGGAGCGT 
CTTTCCTTGC ATGAGGAGAA ACGCACCCCT CCTCCTACTA AGCGCAGTCT GAGTGAAGAG 
GGAGGAGAGC GGATCGACAG TCTAAGTGGC CTAAAGTCCA GAGACCGAAG CTGGTTGGTG 
GGCTCACCTG AAATTCTCAG AAAGCGTCTC TCTGTTTCCG AGTCTTCTCA CACCGAGAGT 
GACTCGAGTC CACCTCTGAC TGTTCGTCGC CGCTGCTGCT CGGCCATCAT TGAAATGCCT 
CGCTTCGCCA TATCCAGTGA AGAGGATAGC TGTGTGGGGA GCAGGAAGAC CCCTGTGAGA 
GGCCCTCGAG GGGATGATCT GCCTCAGGCC ATACCAGAGC TCCCTGTAGA GAGAGAGCTC 
AGGCTGGATG AATCGCCCAC CACGCCAGGA TCCACTTCCA GTCAAATCAG CCAGTCCACA 
CTCACACCTG GTAGTTCTGG TGATGTGTTA GATCGTGCTT CTCGCTATAG TGCTGAAGTT 
TCTGAAAACT CCACTCCAAG AGCCATCAGT GATTTGGCGG CTCGAAGAGC TCGCCACAGA 
CTGCTTTCAG GAGATACAGA TAAACACACA TCACGTCCAC TTAGCAAAGT CATCAAATCA 
GCTTCAGCAA CAACCCTGTC ACTCATGATC CCTGCTGATC ACCATGGTGC TTCTCCACTG 
GCCAGTCCCA TGTCGCCTCA CTCTCTCTCT TCGAACCCCT CATCACGTGA CTCCTCGCCC 
AGCCGTGACC TTTCCCCAGC TGTCTGTAGT GTTAAACCTG CCATAATCAT CCATCGAGCT 
GGAAAGAAAT ATGGTTTTAC TTTACGTGCA ATCCGGGTTT ACATGGGAGA CACAGACATA 
TATACTGTCC ATCACATGGT CTGGCATGTG GAGGATGGAG GACCTGCTCA TGAGGCTGGA 
CTGAGAGAGG GAGATCTCAT CACACATGTT AACGGAGAGC CTGTCCATGG ACTTGTGCAC 
ACAGAAGTTG TTGAGCTCAT TCTTAAGAGT GGCAGTAAGG TGTCCATCTC TGCTACACCC 
TTTGAAAATA CATCAATTAA GGTTGGTCCT GCTCGCAAAA CTAGCTACAA GTCCAAAATG 
GCCCGTCGCA ACAAAAGAAC CAAAACTAGA GAAGGACAAG ACAGTAAGAA GAGGAATTCC 
CTCTTCCGTA AGATCACCAA ACAGGCGACC CTTCTTCACA CCAGTCGCAG CCTGTCCTCT 
CTGAATCGCT CGGTCTCTTC TGGTGAGAGC GTCCCAGGCT CTCCTACACA CATGTCACCT 
CGTTCACCCA CACAGGGCTG TCGTTCAACA CCTGACTCCG CCCACTCAGT TGGTGGAAAC 
TCATCCCAAA GTAGCTCGCC TAGCTCCAGT GTGCCAAACT CTCCTGCCAG CTCTGGCCAG 
ATCAGACCCA GCTCCTTGCA TGGTCTGGCC CCCAAGCTGC AGCGCCAGTA CCGATCCCCC 
CGCCGGAAGT CTGCAGGCAA CATCCCTTTA TCCCCTCTGG CCCGTACACC ATCGCCCACT 
CCACAGAGCA CTTCTCCCCA GCGTTCCCCT TCTCCCCTGC CAAGCCACGG GCTCATCACT 
TCTTCAATAG GCCAGTCTTT TCCTGTCAAA CTGCACTCTT CACCTCCATT AGTAAGACAG 
ATATCTCGGC CAAAGAGTGC AGACCCTCCT CGCTCTCCTC TGCTCAAACG AGTCCAGTCC 
GCAGAGAAAC TCGCCGCCTC TCTTTCTTCT TCATCCTCCT CTCCTTCTCC CTCTTCGGCT 
GCTGATAAAA AGCTTCCAGT TGGTGCCAGC CGCAAACACA GTCTTGATAC CTCACATTCA 
GAGTTTAAAA AAGAGATGCT GCAGCGAGAC CCTAGTCTAC AGAGCCTGCA AGAGTCAGCC 
AGTGAGACTC TTATGGGAGG AAGAGTGTCT CCTGCAGAAA AGGGAAGCCT GCAGAAAAGT 
TCAGTGCGTA AACTGGGCAG GCAAGAAGGT CCCGAGTCTG GAACAGGAAC TCTGGGACTT 
GTTCCTGGAA AGAGTAAATT GAAAGACAAG CTGTCTGCCA TACGTGCTGA ACGCCGCGAA 
TCTCTTCAAA AGCAGGATGC CATCCATGAA GTGGATTCAT CTGAAGATGA GACAGACGAA 
GGGTCGGAAG ACAGCCAAGA TGGGCGCAGA ACTTGCTATG CTCCTCCTAG CCATGTCTTG 
AGGCCTACAA CTGTAATGGC CTCTCCCCAT ACCATCAGAA TGGGGCCAGC GGCTCCACCA 
ACACTGGGAC TTTTTCCTTC AACAGTTGCA CCGCCATGCT CACCTCAGCT CATCAGAGGA 
CTGCAGATAC AAAACCAAAG TACCACTCAA TCTCAGACAC AAACTTCAAC CTCACAACCA 
GCGCAAGCTG CAGTTAAACC CCCAGAACAG AAACCAATAA CCTCTGTTAA AGACTCAAGT 
TCAACGATAA TTGATGATTC GAAAAAACAG TCACAGGCAC AAGACATTCA GTCCAGACCT 
TCTCCACATC AACCCAGTCC TCTGTCTAGT ACCTTTTCCA CCCAAGGCAG CGCCTTTACC 
TCTGTTAGTA AAGACCCATT TGTCAAGCCC ACCACACCTC CATTGGATCC CCGGAGGACC 
CCAAAAATGT CTGAGCCAAG GTCAAAAACC AGTGGTAGAA CTGATTCTAG CACTACTTCT 
GGAGTAGCCA GGGATACCCC AGTTGCGACT ACCCCAACAG GAGGAATCAC TAAGGAGATG 
AAGGAGGCTG ACAATCGCAG ACAGGCTGCA GCTGCTGCTG CTGCCTCTGC CGCCGCTGCT 
ACCACCTCTG CATCCACTGC CTCTGAAAGT GGAATGGATA AAGTTGTGTC TCAGCTCGCC 
ACTGTGGCCA AGAGTGTGCT TGGACCTGTC AAGCTCAATA TCCCAGGTAC TAAAGAGACC 
ATGGAACGAA CAAAGGACCA GCGTACCCAG GCAGATGCCA CCCACCCAAA GATAAAAGAA 
TGTCGCCTGG AGCACCCTGA TTCTCCTGAC CAATTGAGTC CATCTGCTGC TTCGGGCTCC 
CAGTCTCCAC GACTGACTGA ATCTCCATCT AAACAAACAT CATCCAAATC TGAGCCAGCC 
TCAACTTCTC AGAGACCTTT AGAGGTCCCA GGGACTTTCA AAAGACAAAC TTCTCCAATC 
CCTCAAGCTA GGGAAGAGCA TCAGGATAGG TCAGGAACTC TGCAAAGTGG CACCACATCA 
AGGCCTAGGT CTGAAGCACA ATCACAGACC CAGCAGGCAA AGCCCAGTTC CACTACGTCA 
CGAGATAAAT CCAACAAGAC AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_009302496.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300771.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X5, mRNA.

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CloneID	ODa47562
Clone ID Related Accession (Same CDS sequence)	XM_009302496.2 , XM_009302496.3
Accession Version	XM_009302496.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACT CCAGTATATT AAGGCGAAGA GGCCTTCAGA AGGAGCTGAG TCTTCCTAGA 
AGAGGCAGTT TCTGTCGGAC GAGCAACCGA AAAAGTTTGA TAGTGACATC CAGCACGTCT 
CCAACGCTGC CTCGACCCCA TTCACCACTG CACGGGCATC CAGGAAGCAG CCCATTGGAC 
AGCCCTCGCA ATTTCTCCCC CAACACTGCA GCCCACTTCT CTTTTGTACC AGCTCGAAGC 
CATGGACACA GGGCTGACAG GACTGACGGT CGTCGCTGGT CTCTCGCCTC TCTGCCGTCC 
TCTGGCTATG GAACAAACAC CCCCAGTTCG ACAGTCTCGT CATCATGTTC ATCTCAGGAG 
AAGCTGCACC AGTTGCCCTT CCAGCCCACT GCAGACGAGC TGCATTTCCT CACCAAACAC 
TTCAGCTCGG AGAGCATCAC GGATGAGGAT GGGAGACGAT CCCCAGCCAT GCGTCCGCGC 
TCCCGCAGTC TCAGCCCTGG ACGGTCACCA ATATCATTTG ACCATGAGAT TGTCATGATG 
AACCATGTCT ACAAGGAGCG TTTTCCTAAG GCCACTGCTC AGATGGAGGT GCGTCTGGCC 
GATTTCATAT CCTCCTCAGC TCCAGAAAAA GTCATGCCTT TAGCTGATGG CGTCCTGAGC 
TTCATCCACC ACCAGGTTAT CGAACTGTCT CGAGATTGTC TGGACAAATC TCGTGAGGGT 
CTCATCACCT CCCGCTACTT CTATGAGCTA CAAGAGAACC TTGAAAAGCT ACACCAGGAT 
GCTCATGAGC GTTCAGAAAG TGGGGAGGTG ACATTTGTCA CCCAGCTAGT TAAAAAGCTG 
ATGATAATCA TCGCTCGGCC TGCCCGGCTG CTAGAATGTT TGGAGTTTGA CCCGGAGGAG 
TTCTACCACC TGTTAGAGGC AGCTGAAGGT CATGCTAAAG AAGGTCAAGG CATCAAGTCT 
GACATCCCTC GATACATCAT CAGCCAGCTG GGCCTCACCA GGGACCCCCT TGAGGAAATG 
GCCCAGCTGA GCAGCTATGA CAGCGGTAAC CCTGAAACAC CAGAGACAGA GACAGATTCC 
ACAGATAGTC GAGGAGCAAC CGTACAGCCA CTCCAACCTC AGCCTCAACC GCAGCCCAAG 
ACTAAAACAC CTCGCGAGGA AGACTTCGAG ACCATTAAAC TCATCAGTAA TGGAGCCTAT 
GGGGCTGTGT TTCTGGTGAG GCATAAAGAG ACCCGTCAGC GATTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTGATCCT AAGAAACCAG ATCCAGCAGG CCTTTGTGGA AAGAGACATC 
TTGACATTTG CAGAAAACCC TTTTGTTGTC AGCATGTTCT GCTCCTTTGA GACAAGGAGA 
CATCTTTGCA TGGTTATGGA GTATGTGGAG GGTGGGGATT GTGCCACTCT CCTGAAGCAC 
ATTGGAGCTC TGCCTGTGGA TATGGCACGC ATGTACTTTG CTGAAACCGT TCTTGCATTG 
GAATACCTTC ACAACTATGG TATTGTGCAC AGAGACCTCA AACCTGACAA TCTCCTGATT 
ACCTCAATGG GACACGTCAA ACTGACTGAC TTTGGCCTGT CCAAGATTGG TTTAATGAGC 
TTAACAACTA ATCTGTATGA GGGCCACATT GAAAAAGATA CTCGAGAATT CTTGGACAAA 
CAGGTGTGTG GTACTCCTGA ATACATTGCT CCTGAGGTTA TTCTGCGACA GGGTTATGGA 
AAACCAGTTG ACTGGTGGGC CATGGGAGTC ATCTTGTATG AGTTTCTGGT GGGCTGTGCT 
CCTTTCTTTG GAGATACACC TGAAGAACTG TTTGGACAAG TCATCAGTGA TGAGATAATC 
TGGCCAGAAG GTGATGAAGC TTTGCCTCCG GATGCTCAAG ATCTCATCTC CAAACTTCTT 
CGGCAAAACC CTCTAGAGCG TCTTGGGACA GGGAGTGCGT TTGAGGTAAA ACAGCACCGC 
TTCTTTACTG ATCTGGACTG GAACAGCCTC CTGAGGCAGA AGGCTGAGTT CATTCCTCAG 
CTGGAGTCGG AGGATGACAC TAGCTACTTT GACACACGCT CAGATCGTTA TCATCATGTG 
GACTCAGAGG AAGAAGATGA CACAAATGAT GACGACCATT TGGAGATCCG TCAGTTCTCC 
TCCTGTTCGC CACGATTCAG CAAGGTGTAT AGTAGTATGG AGCGTCTTTC CTTGCATGAG 
GAGAAACGCA CCCCTCCTCC TACTAAGCGC AGTCTGAGTG AAGAGGGAGG AGAGCGGATC 
GACAGTCTAA GTGGCCTAAA GTCCAGAGAC CGAAGCTGGT TGGTGGGCTC ACCTGAAATT 
CTCAGAAAGC GTCTCTCTGT TTCCGAGTCT TCTCACACCG AGAGTGACTC GAGTCCACCT 
CTGACTGTTC GTCGCCGCTG CTGCTCGGCC ATCATTGAAA TGCCTCGCTT CGCCATATCC 
AGTGAAGAGG ATAGCTGTGT GGGGAGCAGG AAGACCCCTG TGAGAGGCCC TCGAGGGGAT 
GATCTGCCTC AGGCCATACC AGAGCTCCCT GTAGAGAGAG AGCTCAGGCT GGATGAATCG 
CCCACCACGC CAGGATCCAC TTCCAGTCAA ATCAGCCAGT CCACACTCAC ACCTGGTAGT 
TCTGGTGATG TGTTAGATCG TGCTTCTCGC TATAGTGCTG AAGTTTCTGA AAACTCCACT 
CCAAGAGCCA TCAGTGATTT GGCGGCTCGA AGAGCTCGCC ACAGACTGCT TTCAGGAGAT 
ACAGATAAAC ACACATCACG TCCACTTAGC AAAGTCATCA AATCAGCTTC AGCAACAACC 
CTGTCACTCA TGATCCCTGC TGATCACCAT GGTGCTTCTC CACTGGCCAG TCCCATGTCG 
CCTCACTCTC TCTCTTCGAA CCCCTCATCA CGTGACTCCT CGCCCAGCCG TGACCTTTCC 
CCAGCTGTCT GTAGTGTTAA ACCTGCCATA ATCATCCATC GAGCTGGAAA GAAATATGGT 
TTTACTTTAC GTGCAATCCG GGTTTACATG GGAGACACAG ACATATATAC TGTCCATCAC 
ATGGTCTGGC ATGTGGAGGA TGGAGGACCT GCTCATGAGG CTGGACTGAG AGAGGGAGAT 
CTCATCACAC ATGTTAACGG AGAGCCTGTC CATGGACTTG TGCACACAGA AGTTGTTGAG 
CTCATTCTTA AGAGTGGCAG TAAGGTGTCC ATCTCTGCTA CACCCTTTGA AAATACATCA 
ATTAAGGTTG GTCCTGCTCG CAAAACTAGC TACAAGTCCA AAATGGCCCG TCGCAACAAA 
AGAACCAAAA CTAGAGAAGG ACAAGACAGT AAGAAGAGGA ATTCCCTCTT CCGTAAGATC 
ACCAAACAGG CGACCCTTCT TCACACCAGT CGCAGCCTGT CCTCTCTGAA TCGCTCGGTC 
TCTTCTGGTG AGAGCGTCCC AGGCTCTCCT ACACACATGT CACCTCGTTC ACCCACACAG 
GGCTGTCGTT CAACACCTGA CTCCGCCCAC TCAGTTGGTG GAAACTCATC CCAAAGTAGC 
TCGCCTAGCT CCAGTGTGCC AAACTCTCCT GCCAGCTCTG GCCAGATCAG ACCCAGCTCC 
TTGCATGGTC TGGCCCCCAA GCTGCAGCGC CAGTACCGAT CCCCCCGCCG GAAGTCTGCA 
GGCAACATCC CTTTATCCCC TCTGGCCCGT ACACCATCGC CCACTCCACA GAGCACTTCT 
CCCCAGCGTT CCCCTTCTCC CCTGCCAAGC CACGGGCTCA TCACTTCTTC AATAGGCCAG 
TCTTTTCCTG TCAAACTGCA CTCTTCACCT CCATTAGTAA GACAGATATC TCGGCCAAAG 
AGTGCAGACC CTCCTCGCTC TCCTCTGCTC AAACGAGTCC AGTCCGCAGA GAAACTCGCC 
GCCTCTCTTT CTTCTTCATC CTCCTCTCCT TCTCCCTCTT CGGCTGCTGA TAAAAAGCTT 
CCAGTTGGTG CCAGCCGCAA ACACAGTCTT GATACCTCAC ATTCAGAGTT TAAAAAAGAG 
ATGCTGCAGC GAGACCCTAG TCTACAGAGC CTGCAAGAGT CAGCCAGTGA GACTCTTATG 
GGAGGAAGAG TGTCTCCTGC AGAAAAGGGA AGCCTGCAGA AAAGTTCAGT GCGTAAACTG 
GGCAGGCAAG AAGGTCCCGA GTCTGGAACA GGAACTCTGG GACTTGTTCC TGGAAAGAGT 
AAATTGAAAG ACAAGCTGTC TGCCATACGT GCTGAACGCC GCGAATCTCT TCAAAAGCAG 
GATGCCATCC ATGAAGTGGA TTCATCTGAA GATGAGACAG ACGAAGGGTC GGAAGACAGC 
CAAGATGGGC GCAGAACTTG CTATGCTCCT CCTAGCCATG TCTTGAGGCC TACAACTGTA 
ATGGCCTCTC CCCATACCAT CAGAATGGGG CCAGCGGCTC CACCAACACT GGGACTTTTT 
CCTTCAACAG TTGCACCGCC ATGCTCACCT CAGCTCATCA GAGGACTGCA GATACAAAAC 
CAAAGTACCA CTCAATCTCA GACACAAACT TCAACCTCAC AACCAGCGCA AGCTGCAGTT 
AAACCCCCAG AACAGAAACC AATAACCTCT GTTAAAGACT CAAGTTCAAC GATAATTGAT 
GATTCGAAAA AACAGTCACA GGCACAAGAC ATTCAGTCCA GACCTTCTCC ACATCAACCC 
AGTCCTCTGT CTAGTACCTT TTCCACCCAA GGCAGCGCCT TTACCTCTGT TAGTAAAGAC 
CCATTTGTCA AGCCCACCAC ACCTCCATTG GATCCCCGGA GGACCCCAAA AATGTCTGAG 
CCAAGGTCAA AAACCAGTGG TAGAACTGAT TCTAGCACTA CTTCTGGAGT AGCCAGGGAT 
ACCCCAGTTG CGACTACCCC AACAGGAGGA ATCACTAAGG AGATGAAGGA GGCTGACAAT 
CGCAGACAGG CTGCAGCTGC TGCTGCTGCC TCTGCCGCCG CTGCTACCAC CTCTGCATCC 
ACTGCCTCTG AAAGTGGAAT GGATAAAGTT GTGTCTCAGC TCGCCACTGT GGCCAAGAGT 
GTGCTTGGAC CTGTCAAGCT CAATATCCCA GGTACTAAAG AGACCATGGA ACGAACAAAG 
GACCAGCGTA CCCAGGCAGA TGCCACCCAC CCAAAGATAA AAGAATGTCG CCTGGAGCAC 
CCTGATTCTC CTGACCAATT GAGTCCATCT GCTGCTTCGG GCTCCCAGTC TCCACGACTG 
ACTGAATCTC CATCTAAACA AACATCATCC AAATCTGAGC CAGCCTCAAC TTCTCAGAGA 
CCTTTAGAGG TCCCAGGGAC TTTCAAAAGA CAAACTTCTC CAATCCCTCA AGCTAGGGAA 
GAGCATCAGG ATAGGTCAGG AACTCTGCAA AGTGGCACCA CATCAAGGCC TAGGTCTGAA 
GCACAATCAC AGACCCAGCA GGCAAAGCCC AGTTCCACTA CGTCACGAGA TAAATCCAAC 
AAGACAACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300771.1
CDS	1753..7224
Translation	MDDSSILRRRGLQKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYAPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPCSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_009302496.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302496.2

ORF Insert Sequence:

ATGGATGACT CCAGTATATT AAGGCGAAGA GGCCTTCAGA AGGAGCTGAG TCTTCCTAGA 
AGAGGCAGTT TCTGTCGGAC GAGCAACCGA AAAAGTTTGA TAGTGACATC CAGCACGTCT 
CCAACGCTGC CTCGACCCCA TTCACCACTG CACGGGCATC CAGGAAGCAG CCCATTGGAC 
AGCCCTCGCA ATTTCTCCCC CAACACTGCA GCCCACTTCT CTTTTGTACC AGCTCGAAGC 
CATGGACACA GGGCTGACAG GACTGACGGT CGTCGCTGGT CTCTCGCCTC TCTGCCGTCC 
TCTGGCTATG GAACAAACAC CCCCAGTTCG ACAGTCTCGT CATCATGTTC ATCTCAGGAG 
AAGCTGCACC AGTTGCCCTT CCAGCCCACT GCAGACGAGC TGCATTTCCT CACCAAACAC 
TTCAGCTCGG AGAGCATCAC GGATGAGGAT GGGAGACGAT CCCCAGCCAT GCGTCCGCGC 
TCCCGCAGTC TCAGCCCTGG ACGGTCACCA ATATCATTTG ACCATGAGAT TGTCATGATG 
AACCATGTCT ACAAGGAGCG TTTTCCTAAG GCCACTGCTC AGATGGAGGT GCGTCTGGCC 
GATTTCATAT CCTCCTCAGC TCCAGAAAAA GTCATGCCTT TAGCTGATGG CGTCCTGAGC 
TTCATCCACC ACCAGGTTAT CGAACTGTCT CGAGATTGTC TGGACAAATC TCGTGAGGGT 
CTCATCACCT CCCGCTACTT CTATGAGCTA CAAGAGAACC TTGAAAAGCT ACACCAGGAT 
GCTCATGAGC GTTCAGAAAG TGGGGAGGTG ACATTTGTCA CCCAGCTAGT TAAAAAGCTG 
ATGATAATCA TCGCTCGGCC TGCCCGGCTG CTAGAATGTT TGGAGTTTGA CCCGGAGGAG 
TTCTACCACC TGTTAGAGGC AGCTGAAGGT CATGCTAAAG AAGGTCAAGG CATCAAGTCT 
GACATCCCTC GATACATCAT CAGCCAGCTG GGCCTCACCA GGGACCCCCT TGAGGAAATG 
GCCCAGCTGA GCAGCTATGA CAGCGGTAAC CCTGAAACAC CAGAGACAGA GACAGATTCC 
ACAGATAGTC GAGGAGCAAC CGTACAGCCA CTCCAACCTC AGCCTCAACC GCAGCCCAAG 
ACTAAAACAC CTCGCGAGGA AGACTTCGAG ACCATTAAAC TCATCAGTAA TGGAGCCTAT 
GGGGCTGTGT TTCTGGTGAG GCATAAAGAG ACCCGTCAGC GATTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTGATCCT AAGAAACCAG ATCCAGCAGG CCTTTGTGGA AAGAGACATC 
TTGACATTTG CAGAAAACCC TTTTGTTGTC AGCATGTTCT GCTCCTTTGA GACAAGGAGA 
CATCTTTGCA TGGTTATGGA GTATGTGGAG GGTGGGGATT GTGCCACTCT CCTGAAGCAC 
ATTGGAGCTC TGCCTGTGGA TATGGCACGC ATGTACTTTG CTGAAACCGT TCTTGCATTG 
GAATACCTTC ACAACTATGG TATTGTGCAC AGAGACCTCA AACCTGACAA TCTCCTGATT 
ACCTCAATGG GACACGTCAA ACTGACTGAC TTTGGCCTGT CCAAGATTGG TTTAATGAGC 
TTAACAACTA ATCTGTATGA GGGCCACATT GAAAAAGATA CTCGAGAATT CTTGGACAAA 
CAGGTGTGTG GTACTCCTGA ATACATTGCT CCTGAGGTTA TTCTGCGACA GGGTTATGGA 
AAACCAGTTG ACTGGTGGGC CATGGGAGTC ATCTTGTATG AGTTTCTGGT GGGCTGTGCT 
CCTTTCTTTG GAGATACACC TGAAGAACTG TTTGGACAAG TCATCAGTGA TGAGATAATC 
TGGCCAGAAG GTGATGAAGC TTTGCCTCCG GATGCTCAAG ATCTCATCTC CAAACTTCTT 
CGGCAAAACC CTCTAGAGCG TCTTGGGACA GGGAGTGCGT TTGAGGTAAA ACAGCACCGC 
TTCTTTACTG ATCTGGACTG GAACAGCCTC CTGAGGCAGA AGGCTGAGTT CATTCCTCAG 
CTGGAGTCGG AGGATGACAC TAGCTACTTT GACACACGCT CAGATCGTTA TCATCATGTG 
GACTCAGAGG AAGAAGATGA CACAAATGAT GACGACCATT TGGAGATCCG TCAGTTCTCC 
TCCTGTTCGC CACGATTCAG CAAGGTGTAT AGTAGTATGG AGCGTCTTTC CTTGCATGAG 
GAGAAACGCA CCCCTCCTCC TACTAAGCGC AGTCTGAGTG AAGAGGGAGG AGAGCGGATC 
GACAGTCTAA GTGGCCTAAA GTCCAGAGAC CGAAGCTGGT TGGTGGGCTC ACCTGAAATT 
CTCAGAAAGC GTCTCTCTGT TTCCGAGTCT TCTCACACCG AGAGTGACTC GAGTCCACCT 
CTGACTGTTC GTCGCCGCTG CTGCTCGGCC ATCATTGAAA TGCCTCGCTT CGCCATATCC 
AGTGAAGAGG ATAGCTGTGT GGGGAGCAGG AAGACCCCTG TGAGAGGCCC TCGAGGGGAT 
GATCTGCCTC AGGCCATACC AGAGCTCCCT GTAGAGAGAG AGCTCAGGCT GGATGAATCG 
CCCACCACGC CAGGATCCAC TTCCAGTCAA ATCAGCCAGT CCACACTCAC ACCTGGTAGT 
TCTGGTGATG TGTTAGATCG TGCTTCTCGC TATAGTGCTG AAGTTTCTGA AAACTCCACT 
CCAAGAGCCA TCAGTGATTT GGCGGCTCGA AGAGCTCGCC ACAGACTGCT TTCAGGAGAT 
ACAGATAAAC ACACATCACG TCCACTTAGC AAAGTCATCA AATCAGCTTC AGCAACAACC 
CTGTCACTCA TGATCCCTGC TGATCACCAT GGTGCTTCTC CACTGGCCAG TCCCATGTCG 
CCTCACTCTC TCTCTTCGAA CCCCTCATCA CGTGACTCCT CGCCCAGCCG TGACCTTTCC 
CCAGCTGTCT GTAGTGTTAA ACCTGCCATA ATCATCCATC GAGCTGGAAA GAAATATGGT 
TTTACTTTAC GTGCAATCCG GGTTTACATG GGAGACACAG ACATATATAC TGTCCATCAC 
ATGGTCTGGC ATGTGGAGGA TGGAGGACCT GCTCATGAGG CTGGACTGAG AGAGGGAGAT 
CTCATCACAC ATGTTAACGG AGAGCCTGTC CATGGACTTG TGCACACAGA AGTTGTTGAG 
CTCATTCTTA AGAGTGGCAG TAAGGTGTCC ATCTCTGCTA CACCCTTTGA AAATACATCA 
ATTAAGGTTG GTCCTGCTCG CAAAACTAGC TACAAGTCCA AAATGGCCCG TCGCAACAAA 
AGAACCAAAA CTAGAGAAGG ACAAGACAGT AAGAAGAGGA ATTCCCTCTT CCGTAAGATC 
ACCAAACAGG CGACCCTTCT TCACACCAGT CGCAGCCTGT CCTCTCTGAA TCGCTCGGTC 
TCTTCTGGTG AGAGCGTCCC AGGCTCTCCT ACACACATGT CACCTCGTTC ACCCACACAG 
GGCTGTCGTT CAACACCTGA CTCCGCCCAC TCAGTTGGTG GAAACTCATC CCAAAGTAGC 
TCGCCTAGCT CCAGTGTGCC AAACTCTCCT GCCAGCTCTG GCCAGATCAG ACCCAGCTCC 
TTGCATGGTC TGGCCCCCAA GCTGCAGCGC CAGTACCGAT CCCCCCGCCG GAAGTCTGCA 
GGCAACATCC CTTTATCCCC TCTGGCCCGT ACACCATCGC CCACTCCACA GAGCACTTCT 
CCCCAGCGTT CCCCTTCTCC CCTGCCAAGC CACGGGCTCA TCACTTCTTC AATAGGCCAG 
TCTTTTCCTG TCAAACTGCA CTCTTCACCT CCATTAGTAA GACAGATATC TCGGCCAAAG 
AGTGCAGACC CTCCTCGCTC TCCTCTGCTC AAACGAGTCC AGTCCGCAGA GAAACTCGCC 
GCCTCTCTTT CTTCTTCATC CTCCTCTCCT TCTCCCTCTT CGGCTGCTGA TAAAAAGCTT 
CCAGTTGGTG CCAGCCGCAA ACACAGTCTT GATACCTCAC ATTCAGAGTT TAAAAAAGAG 
ATGCTGCAGC GAGACCCTAG TCTACAGAGC CTGCAAGAGT CAGCCAGTGA GACTCTTATG 
GGAGGAAGAG TGTCTCCTGC AGAAAAGGGA AGCCTGCAGA AAAGTTCAGT GCGTAAACTG 
GGCAGGCAAG AAGGTCCCGA GTCTGGAACA GGAACTCTGG GACTTGTTCC TGGAAAGAGT 
AAATTGAAAG ACAAGCTGTC TGCCATACGT GCTGAACGCC GCGAATCTCT TCAAAAGCAG 
GATGCCATCC ATGAAGTGGA TTCATCTGAA GATGAGACAG ACGAAGGGTC GGAAGACAGC 
CAAGATGGGC GCAGAACTTG CTATGCTCCT CCTAGCCATG TCTTGAGGCC TACAACTGTA 
ATGGCCTCTC CCCATACCAT CAGAATGGGG CCAGCGGCTC CACCAACACT GGGACTTTTT 
CCTTCAACAG TTGCACCGCC ATGCTCACCT CAGCTCATCA GAGGACTGCA GATACAAAAC 
CAAAGTACCA CTCAATCTCA GACACAAACT TCAACCTCAC AACCAGCGCA AGCTGCAGTT 
AAACCCCCAG AACAGAAACC AATAACCTCT GTTAAAGACT CAAGTTCAAC GATAATTGAT 
GATTCGAAAA AACAGTCACA GGCACAAGAC ATTCAGTCCA GACCTTCTCC ACATCAACCC 
AGTCCTCTGT CTAGTACCTT TTCCACCCAA GGCAGCGCCT TTACCTCTGT TAGTAAAGAC 
CCATTTGTCA AGCCCACCAC ACCTCCATTG GATCCCCGGA GGACCCCAAA AATGTCTGAG 
CCAAGGTCAA AAACCAGTGG TAGAACTGAT TCTAGCACTA CTTCTGGAGT AGCCAGGGAT 
ACCCCAGTTG CGACTACCCC AACAGGAGGA ATCACTAAGG AGATGAAGGA GGCTGACAAT 
CGCAGACAGG CTGCAGCTGC TGCTGCTGCC TCTGCCGCCG CTGCTACCAC CTCTGCATCC 
ACTGCCTCTG AAAGTGGAAT GGATAAAGTT GTGTCTCAGC TCGCCACTGT GGCCAAGAGT 
GTGCTTGGAC CTGTCAAGCT CAATATCCCA GGTACTAAAG AGACCATGGA ACGAACAAAG 
GACCAGCGTA CCCAGGCAGA TGCCACCCAC CCAAAGATAA AAGAATGTCG CCTGGAGCAC 
CCTGATTCTC CTGACCAATT GAGTCCATCT GCTGCTTCGG GCTCCCAGTC TCCACGACTG 
ACTGAATCTC CATCTAAACA AACATCATCC AAATCTGAGC CAGCCTCAAC TTCTCAGAGA 
CCTTTAGAGG TCCCAGGGAC TTTCAAAAGA CAAACTTCTC CAATCCCTCA AGCTAGGGAA 
GAGCATCAGG ATAGGTCAGG AACTCTGCAA AGTGGCACCA CATCAAGGCC TAGGTCTGAA 
GCACAATCAC AGACCCAGCA GGCAAAGCCC AGTTCCACTA CGTCACGAGA TAAATCCAAC 
AAGACAACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mast2 ( XM_009302496.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300771.1 Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X2, mRNA.

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CloneID	ODa47562
Clone ID Related Accession (Same CDS sequence)	XM_009302496.2 , XM_009302496.3
Accession Version	XM_009302496.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302496.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACT CCAGTATATT AAGGCGAAGA GGCCTTCAGA AGGAGCTGAG TCTTCCTAGA 
AGAGGCAGTT TCTGTCGGAC GAGCAACCGA AAAAGTTTGA TAGTGACATC CAGCACGTCT 
CCAACGCTGC CTCGACCCCA TTCACCACTG CACGGGCATC CAGGAAGCAG CCCATTGGAC 
AGCCCTCGCA ATTTCTCCCC CAACACTGCA GCCCACTTCT CTTTTGTACC AGCTCGAAGC 
CATGGACACA GGGCTGACAG GACTGACGGT CGTCGCTGGT CTCTCGCCTC TCTGCCGTCC 
TCTGGCTATG GAACAAACAC CCCCAGTTCG ACAGTCTCGT CATCATGTTC ATCTCAGGAG 
AAGCTGCACC AGTTGCCCTT CCAGCCCACT GCAGACGAGC TGCATTTCCT CACCAAACAC 
TTCAGCTCGG AGAGCATCAC GGATGAGGAT GGGAGACGAT CCCCAGCCAT GCGTCCGCGC 
TCCCGCAGTC TCAGCCCTGG ACGGTCACCA ATATCATTTG ACCATGAGAT TGTCATGATG 
AACCATGTCT ACAAGGAGCG TTTTCCTAAG GCCACTGCTC AGATGGAGGT GCGTCTGGCC 
GATTTCATAT CCTCCTCAGC TCCAGAAAAA GTCATGCCTT TAGCTGATGG CGTCCTGAGC 
TTCATCCACC ACCAGGTTAT CGAACTGTCT CGAGATTGTC TGGACAAATC TCGTGAGGGT 
CTCATCACCT CCCGCTACTT CTATGAGCTA CAAGAGAACC TTGAAAAGCT ACACCAGGAT 
GCTCATGAGC GTTCAGAAAG TGGGGAGGTG ACATTTGTCA CCCAGCTAGT TAAAAAGCTG 
ATGATAATCA TCGCTCGGCC TGCCCGGCTG CTAGAATGTT TGGAGTTTGA CCCGGAGGAG 
TTCTACCACC TGTTAGAGGC AGCTGAAGGT CATGCTAAAG AAGGTCAAGG CATCAAGTCT 
GACATCCCTC GATACATCAT CAGCCAGCTG GGCCTCACCA GGGACCCCCT TGAGGAAATG 
GCCCAGCTGA GCAGCTATGA CAGCGGTAAC CCTGAAACAC CAGAGACAGA GACAGATTCC 
ACAGATAGTC GAGGAGCAAC CGTACAGCCA CTCCAACCTC AGCCTCAACC GCAGCCCAAG 
ACTAAAACAC CTCGCGAGGA AGACTTCGAG ACCATTAAAC TCATCAGTAA TGGAGCCTAT 
GGGGCTGTGT TTCTGGTGAG GCATAAAGAG ACCCGTCAGC GATTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTGATCCT AAGAAACCAG ATCCAGCAGG CCTTTGTGGA AAGAGACATC 
TTGACATTTG CAGAAAACCC TTTTGTTGTC AGCATGTTCT GCTCCTTTGA GACAAGGAGA 
CATCTTTGCA TGGTTATGGA GTATGTGGAG GGTGGGGATT GTGCCACTCT CCTGAAGCAC 
ATTGGAGCTC TGCCTGTGGA TATGGCACGC ATGTACTTTG CTGAAACCGT TCTTGCATTG 
GAATACCTTC ACAACTATGG TATTGTGCAC AGAGACCTCA AACCTGACAA TCTCCTGATT 
ACCTCAATGG GACACGTCAA ACTGACTGAC TTTGGCCTGT CCAAGATTGG TTTAATGAGC 
TTAACAACTA ATCTGTATGA GGGCCACATT GAAAAAGATA CTCGAGAATT CTTGGACAAA 
CAGGTGTGTG GTACTCCTGA ATACATTGCT CCTGAGGTTA TTCTGCGACA GGGTTATGGA 
AAACCAGTTG ACTGGTGGGC CATGGGAGTC ATCTTGTATG AGTTTCTGGT GGGCTGTGCT 
CCTTTCTTTG GAGATACACC TGAAGAACTG TTTGGACAAG TCATCAGTGA TGAGATAATC 
TGGCCAGAAG GTGATGAAGC TTTGCCTCCG GATGCTCAAG ATCTCATCTC CAAACTTCTT 
CGGCAAAACC CTCTAGAGCG TCTTGGGACA GGGAGTGCGT TTGAGGTAAA ACAGCACCGC 
TTCTTTACTG ATCTGGACTG GAACAGCCTC CTGAGGCAGA AGGCTGAGTT CATTCCTCAG 
CTGGAGTCGG AGGATGACAC TAGCTACTTT GACACACGCT CAGATCGTTA TCATCATGTG 
GACTCAGAGG AAGAAGATGA CACAAATGAT GACGACCATT TGGAGATCCG TCAGTTCTCC 
TCCTGTTCGC CACGATTCAG CAAGGTGTAT AGTAGTATGG AGCGTCTTTC CTTGCATGAG 
GAGAAACGCA CCCCTCCTCC TACTAAGCGC AGTCTGAGTG AAGAGGGAGG AGAGCGGATC 
GACAGTCTAA GTGGCCTAAA GTCCAGAGAC CGAAGCTGGT TGGTGGGCTC ACCTGAAATT 
CTCAGAAAGC GTCTCTCTGT TTCCGAGTCT TCTCACACCG AGAGTGACTC GAGTCCACCT 
CTGACTGTTC GTCGCCGCTG CTGCTCGGCC ATCATTGAAA TGCCTCGCTT CGCCATATCC 
AGTGAAGAGG ATAGCTGTGT GGGGAGCAGG AAGACCCCTG TGAGAGGCCC TCGAGGGGAT 
GATCTGCCTC AGGCCATACC AGAGCTCCCT GTAGAGAGAG AGCTCAGGCT GGATGAATCG 
CCCACCACGC CAGGATCCAC TTCCAGTCAA ATCAGCCAGT CCACACTCAC ACCTGGTAGT 
TCTGGTGATG TGTTAGATCG TGCTTCTCGC TATAGTGCTG AAGTTTCTGA AAACTCCACT 
CCAAGAGCCA TCAGTGATTT GGCGGCTCGA AGAGCTCGCC ACAGACTGCT TTCAGGAGAT 
ACAGATAAAC ACACATCACG TCCACTTAGC AAAGTCATCA AATCAGCTTC AGCAACAACC 
CTGTCACTCA TGATCCCTGC TGATCACCAT GGTGCTTCTC CACTGGCCAG TCCCATGTCG 
CCTCACTCTC TCTCTTCGAA CCCCTCATCA CGTGACTCCT CGCCCAGCCG TGACCTTTCC 
CCAGCTGTCT GTAGTGTTAA ACCTGCCATA ATCATCCATC GAGCTGGAAA GAAATATGGT 
TTTACTTTAC GTGCAATCCG GGTTTACATG GGAGACACAG ACATATATAC TGTCCATCAC 
ATGGTCTGGC ATGTGGAGGA TGGAGGACCT GCTCATGAGG CTGGACTGAG AGAGGGAGAT 
CTCATCACAC ATGTTAACGG AGAGCCTGTC CATGGACTTG TGCACACAGA AGTTGTTGAG 
CTCATTCTTA AGAGTGGCAG TAAGGTGTCC ATCTCTGCTA CACCCTTTGA AAATACATCA 
ATTAAGGTTG GTCCTGCTCG CAAAACTAGC TACAAGTCCA AAATGGCCCG TCGCAACAAA 
AGAACCAAAA CTAGAGAAGG ACAAGACAGT AAGAAGAGGA ATTCCCTCTT CCGTAAGATC 
ACCAAACAGG CGACCCTTCT TCACACCAGT CGCAGCCTGT CCTCTCTGAA TCGCTCGGTC 
TCTTCTGGTG AGAGCGTCCC AGGCTCTCCT ACACACATGT CACCTCGTTC ACCCACACAG 
GGCTGTCGTT CAACACCTGA CTCCGCCCAC TCAGTTGGTG GAAACTCATC CCAAAGTAGC 
TCGCCTAGCT CCAGTGTGCC AAACTCTCCT GCCAGCTCTG GCCAGATCAG ACCCAGCTCC 
TTGCATGGTC TGGCCCCCAA GCTGCAGCGC CAGTACCGAT CCCCCCGCCG GAAGTCTGCA 
GGCAACATCC CTTTATCCCC TCTGGCCCGT ACACCATCGC CCACTCCACA GAGCACTTCT 
CCCCAGCGTT CCCCTTCTCC CCTGCCAAGC CACGGGCTCA TCACTTCTTC AATAGGCCAG 
TCTTTTCCTG TCAAACTGCA CTCTTCACCT CCATTAGTAA GACAGATATC TCGGCCAAAG 
AGTGCAGACC CTCCTCGCTC TCCTCTGCTC AAACGAGTCC AGTCCGCAGA GAAACTCGCC 
GCCTCTCTTT CTTCTTCATC CTCCTCTCCT TCTCCCTCTT CGGCTGCTGA TAAAAAGCTT 
CCAGTTGGTG CCAGCCGCAA ACACAGTCTT GATACCTCAC ATTCAGAGTT TAAAAAAGAG 
ATGCTGCAGC GAGACCCTAG TCTACAGAGC CTGCAAGAGT CAGCCAGTGA GACTCTTATG 
GGAGGAAGAG TGTCTCCTGC AGAAAAGGGA AGCCTGCAGA AAAGTTCAGT GCGTAAACTG 
GGCAGGCAAG AAGGTCCCGA GTCTGGAACA GGAACTCTGG GACTTGTTCC TGGAAAGAGT 
AAATTGAAAG ACAAGCTGTC TGCCATACGT GCTGAACGCC GCGAATCTCT TCAAAAGCAG 
GATGCCATCC ATGAAGTGGA TTCATCTGAA GATGAGACAG ACGAAGGGTC GGAAGACAGC 
CAAGATGGGC GCAGAACTTG CTATGCTCCT CCTAGCCATG TCTTGAGGCC TACAACTGTA 
ATGGCCTCTC CCCATACCAT CAGAATGGGG CCAGCGGCTC CACCAACACT GGGACTTTTT 
CCTTCAACAG TTGCACCGCC ATGCTCACCT CAGCTCATCA GAGGACTGCA GATACAAAAC 
CAAAGTACCA CTCAATCTCA GACACAAACT TCAACCTCAC AACCAGCGCA AGCTGCAGTT 
AAACCCCCAG AACAGAAACC AATAACCTCT GTTAAAGACT CAAGTTCAAC GATAATTGAT 
GATTCGAAAA AACAGTCACA GGCACAAGAC ATTCAGTCCA GACCTTCTCC ACATCAACCC 
AGTCCTCTGT CTAGTACCTT TTCCACCCAA GGCAGCGCCT TTACCTCTGT TAGTAAAGAC 
CCATTTGTCA AGCCCACCAC ACCTCCATTG GATCCCCGGA GGACCCCAAA AATGTCTGAG 
CCAAGGTCAA AAACCAGTGG TAGAACTGAT TCTAGCACTA CTTCTGGAGT AGCCAGGGAT 
ACCCCAGTTG CGACTACCCC AACAGGAGGA ATCACTAAGG AGATGAAGGA GGCTGACAAT 
CGCAGACAGG CTGCAGCTGC TGCTGCTGCC TCTGCCGCCG CTGCTACCAC CTCTGCATCC 
ACTGCCTCTG AAAGTGGAAT GGATAAAGTT GTGTCTCAGC TCGCCACTGT GGCCAAGAGT 
GTGCTTGGAC CTGTCAAGCT CAATATCCCA GGTACTAAAG AGACCATGGA ACGAACAAAG 
GACCAGCGTA CCCAGGCAGA TGCCACCCAC CCAAAGATAA AAGAATGTCG CCTGGAGCAC 
CCTGATTCTC CTGACCAATT GAGTCCATCT GCTGCTTCGG GCTCCCAGTC TCCACGACTG 
ACTGAATCTC CATCTAAACA AACATCATCC AAATCTGAGC CAGCCTCAAC TTCTCAGAGA 
CCTTTAGAGG TCCCAGGGAC TTTCAAAAGA CAAACTTCTC CAATCCCTCA AGCTAGGGAA 
GAGCATCAGG ATAGGTCAGG AACTCTGCAA AGTGGCACCA CATCAAGGCC TAGGTCTGAA 
GCACAATCAC AGACCCAGCA GGCAAAGCCC AGTTCCACTA CGTCACGAGA TAAATCCAAC 
AAGACAACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300771.1
CDS	1632..7103
Translation	MDDSSILRRRGLQKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHPGSSPLDSPRNFSPNTAAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSSESITDEDGRRSPAMRPRSRSLSPGRSPISFDHEIVMMNHVYKERFPKATAQMEVRLADFISSSAPEKVMPLADGVLSFIHHQVIELSRDCLDKSREGLITSRYFYELQENLEKLHQDAHERSESGEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEEMAQLSSYDSGNPETPETETDSTDSRGATVQPLQPQPQPQPKTKTPREEDFETIKLISNGAYGAVFLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEGDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAEFIPQLESEDDTSYFDTRSDRYHHVDSEEEDDTNDDDHLEIRQFSSCSPRFSKVYSSMERLSLHEEKRTPPPTKRSLSEEGGERIDSLSGLKSRDRSWLVGSPEILRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISSEEDSCVGSRKTPVRGPRGDDLPQAIPELPVERELRLDESPTTPGSTSSQISQSTLTPGSSGDVLDRASRYSAEVSENSTPRAISDLAARRARHRLLSGDTDKHTSRPLSKVIKSASATTLSLMIPADHHGASPLASPMSPHSLSSNPSSRDSSPSRDLSPAVCSVKPAIIIHRAGKKYGFTLRAIRVYMGDTDIYTVHHMVWHVEDGGPAHEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGSKVSISATPFENTSIKVGPARKTSYKSKMARRNKRTKTREGQDSKKRNSLFRKITKQATLLHTSRSLSSLNRSVSSGESVPGSPTHMSPRSPTQGCRSTPDSAHSVGGNSSQSSSPSSSVPNSPASSGQIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLARTPSPTPQSTSPQRSPSPLPSHGLITSSIGQSFPVKLHSSPPLVRQISRPKSADPPRSPLLKRVQSAEKLAASLSSSSSSPSPSSAADKKLPVGASRKHSLDTSHSEFKKEMLQRDPSLQSLQESASETLMGGRVSPAEKGSLQKSSVRKLGRQEGPESGTGTLGLVPGKSKLKDKLSAIRAERRESLQKQDAIHEVDSSEDETDEGSEDSQDGRRTCYAPPSHVLRPTTVMASPHTIRMGPAAPPTLGLFPSTVAPPCSPQLIRGLQIQNQSTTQSQTQTSTSQPAQAAVKPPEQKPITSVKDSSSTIIDDSKKQSQAQDIQSRPSPHQPSPLSSTFSTQGSAFTSVSKDPFVKPTTPPLDPRRTPKMSEPRSKTSGRTDSSTTSGVARDTPVATTPTGGITKEMKEADNRRQAAAAAAASAAAATTSASTASESGMDKVVSQLATVAKSVLGPVKLNIPGTKETMERTKDQRTQADATHPKIKECRLEHPDSPDQLSPSAASGSQSPRLTESPSKQTSSKSEPASTSQRPLEVPGTFKRQTSPIPQAREEHQDRSGTLQSGTTSRPRSEAQSQTQQAKPSSTTSRDKSNKTT

Target ORF information:

RefSeq Version	XM_009302496.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase 2 (mast2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302496.3

ORF Insert Sequence:

ATGGATGACT CCAGTATATT AAGGCGAAGA GGCCTTCAGA AGGAGCTGAG TCTTCCTAGA 
AGAGGCAGTT TCTGTCGGAC GAGCAACCGA AAAAGTTTGA TAGTGACATC CAGCACGTCT 
CCAACGCTGC CTCGACCCCA TTCACCACTG CACGGGCATC CAGGAAGCAG CCCATTGGAC 
AGCCCTCGCA ATTTCTCCCC CAACACTGCA GCCCACTTCT CTTTTGTACC AGCTCGAAGC 
CATGGACACA GGGCTGACAG GACTGACGGT CGTCGCTGGT CTCTCGCCTC TCTGCCGTCC 
TCTGGCTATG GAACAAACAC CCCCAGTTCG ACAGTCTCGT CATCATGTTC ATCTCAGGAG 
AAGCTGCACC AGTTGCCCTT CCAGCCCACT GCAGACGAGC TGCATTTCCT CACCAAACAC 
TTCAGCTCGG AGAGCATCAC GGATGAGGAT GGGAGACGAT CCCCAGCCAT GCGTCCGCGC 
TCCCGCAGTC TCAGCCCTGG ACGGTCACCA ATATCATTTG ACCATGAGAT TGTCATGATG 
AACCATGTCT ACAAGGAGCG TTTTCCTAAG GCCACTGCTC AGATGGAGGT GCGTCTGGCC 
GATTTCATAT CCTCCTCAGC TCCAGAAAAA GTCATGCCTT TAGCTGATGG CGTCCTGAGC 
TTCATCCACC ACCAGGTTAT CGAACTGTCT CGAGATTGTC TGGACAAATC TCGTGAGGGT 
CTCATCACCT CCCGCTACTT CTATGAGCTA CAAGAGAACC TTGAAAAGCT ACACCAGGAT 
GCTCATGAGC GTTCAGAAAG TGGGGAGGTG ACATTTGTCA CCCAGCTAGT TAAAAAGCTG 
ATGATAATCA TCGCTCGGCC TGCCCGGCTG CTAGAATGTT TGGAGTTTGA CCCGGAGGAG 
TTCTACCACC TGTTAGAGGC AGCTGAAGGT CATGCTAAAG AAGGTCAAGG CATCAAGTCT 
GACATCCCTC GATACATCAT CAGCCAGCTG GGCCTCACCA GGGACCCCCT TGAGGAAATG 
GCCCAGCTGA GCAGCTATGA CAGCGGTAAC CCTGAAACAC CAGAGACAGA GACAGATTCC 
ACAGATAGTC GAGGAGCAAC CGTACAGCCA CTCCAACCTC AGCCTCAACC GCAGCCCAAG 
ACTAAAACAC CTCGCGAGGA AGACTTCGAG ACCATTAAAC TCATCAGTAA TGGAGCCTAT 
GGGGCTGTGT TTCTGGTGAG GCATAAAGAG ACCCGTCAGC GATTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTGATCCT AAGAAACCAG ATCCAGCAGG CCTTTGTGGA AAGAGACATC 
TTGACATTTG CAGAAAACCC TTTTGTTGTC AGCATGTTCT GCTCCTTTGA GACAAGGAGA 
CATCTTTGCA TGGTTATGGA GTATGTGGAG GGTGGGGATT GTGCCACTCT CCTGAAGCAC 
ATTGGAGCTC TGCCTGTGGA TATGGCACGC ATGTACTTTG CTGAAACCGT TCTTGCATTG 
GAATACCTTC ACAACTATGG TATTGTGCAC AGAGACCTCA AACCTGACAA TCTCCTGATT 
ACCTCAATGG GACACGTCAA ACTGACTGAC TTTGGCCTGT CCAAGATTGG TTTAATGAGC 
TTAACAACTA ATCTGTATGA GGGCCACATT GAAAAAGATA CTCGAGAATT CTTGGACAAA 
CAGGTGTGTG GTACTCCTGA ATACATTGCT CCTGAGGTTA TTCTGCGACA GGGTTATGGA 
AAACCAGTTG ACTGGTGGGC CATGGGAGTC ATCTTGTATG AGTTTCTGGT GGGCTGTGCT 
CCTTTCTTTG GAGATACACC TGAAGAACTG TTTGGACAAG TCATCAGTGA TGAGATAATC 
TGGCCAGAAG GTGATGAAGC TTTGCCTCCG GATGCTCAAG ATCTCATCTC CAAACTTCTT 
CGGCAAAACC CTCTAGAGCG TCTTGGGACA GGGAGTGCGT TTGAGGTAAA ACAGCACCGC 
TTCTTTACTG ATCTGGACTG GAACAGCCTC CTGAGGCAGA AGGCTGAGTT CATTCCTCAG 
CTGGAGTCGG AGGATGACAC TAGCTACTTT GACACACGCT CAGATCGTTA TCATCATGTG 
GACTCAGAGG AAGAAGATGA CACAAATGAT GACGACCATT TGGAGATCCG TCAGTTCTCC 
TCCTGTTCGC CACGATTCAG CAAGGTGTAT AGTAGTATGG AGCGTCTTTC CTTGCATGAG 
GAGAAACGCA CCCCTCCTCC TACTAAGCGC AGTCTGAGTG AAGAGGGAGG AGAGCGGATC 
GACAGTCTAA GTGGCCTAAA GTCCAGAGAC CGAAGCTGGT TGGTGGGCTC ACCTGAAATT 
CTCAGAAAGC GTCTCTCTGT TTCCGAGTCT TCTCACACCG AGAGTGACTC GAGTCCACCT 
CTGACTGTTC GTCGCCGCTG CTGCTCGGCC ATCATTGAAA TGCCTCGCTT CGCCATATCC 
AGTGAAGAGG ATAGCTGTGT GGGGAGCAGG AAGACCCCTG TGAGAGGCCC TCGAGGGGAT 
GATCTGCCTC AGGCCATACC AGAGCTCCCT GTAGAGAGAG AGCTCAGGCT GGATGAATCG 
CCCACCACGC CAGGATCCAC TTCCAGTCAA ATCAGCCAGT CCACACTCAC ACCTGGTAGT 
TCTGGTGATG TGTTAGATCG TGCTTCTCGC TATAGTGCTG AAGTTTCTGA AAACTCCACT 
CCAAGAGCCA TCAGTGATTT GGCGGCTCGA AGAGCTCGCC ACAGACTGCT TTCAGGAGAT 
ACAGATAAAC ACACATCACG TCCACTTAGC AAAGTCATCA AATCAGCTTC AGCAACAACC 
CTGTCACTCA TGATCCCTGC TGATCACCAT GGTGCTTCTC CACTGGCCAG TCCCATGTCG 
CCTCACTCTC TCTCTTCGAA CCCCTCATCA CGTGACTCCT CGCCCAGCCG TGACCTTTCC 
CCAGCTGTCT GTAGTGTTAA ACCTGCCATA ATCATCCATC GAGCTGGAAA GAAATATGGT 
TTTACTTTAC GTGCAATCCG GGTTTACATG GGAGACACAG ACATATATAC TGTCCATCAC 
ATGGTCTGGC ATGTGGAGGA TGGAGGACCT GCTCATGAGG CTGGACTGAG AGAGGGAGAT 
CTCATCACAC ATGTTAACGG AGAGCCTGTC CATGGACTTG TGCACACAGA AGTTGTTGAG 
CTCATTCTTA AGAGTGGCAG TAAGGTGTCC ATCTCTGCTA CACCCTTTGA AAATACATCA 
ATTAAGGTTG GTCCTGCTCG CAAAACTAGC TACAAGTCCA AAATGGCCCG TCGCAACAAA 
AGAACCAAAA CTAGAGAAGG ACAAGACAGT AAGAAGAGGA ATTCCCTCTT CCGTAAGATC 
ACCAAACAGG CGACCCTTCT TCACACCAGT CGCAGCCTGT CCTCTCTGAA TCGCTCGGTC 
TCTTCTGGTG AGAGCGTCCC AGGCTCTCCT ACACACATGT CACCTCGTTC ACCCACACAG 
GGCTGTCGTT CAACACCTGA CTCCGCCCAC TCAGTTGGTG GAAACTCATC CCAAAGTAGC 
TCGCCTAGCT CCAGTGTGCC AAACTCTCCT GCCAGCTCTG GCCAGATCAG ACCCAGCTCC 
TTGCATGGTC TGGCCCCCAA GCTGCAGCGC CAGTACCGAT CCCCCCGCCG GAAGTCTGCA 
GGCAACATCC CTTTATCCCC TCTGGCCCGT ACACCATCGC CCACTCCACA GAGCACTTCT 
CCCCAGCGTT CCCCTTCTCC CCTGCCAAGC CACGGGCTCA TCACTTCTTC AATAGGCCAG 
TCTTTTCCTG TCAAACTGCA CTCTTCACCT CCATTAGTAA GACAGATATC TCGGCCAAAG 
AGTGCAGACC CTCCTCGCTC TCCTCTGCTC AAACGAGTCC AGTCCGCAGA GAAACTCGCC 
GCCTCTCTTT CTTCTTCATC CTCCTCTCCT TCTCCCTCTT CGGCTGCTGA TAAAAAGCTT 
CCAGTTGGTG CCAGCCGCAA ACACAGTCTT GATACCTCAC ATTCAGAGTT TAAAAAAGAG 
ATGCTGCAGC GAGACCCTAG TCTACAGAGC CTGCAAGAGT CAGCCAGTGA GACTCTTATG 
GGAGGAAGAG TGTCTCCTGC AGAAAAGGGA AGCCTGCAGA AAAGTTCAGT GCGTAAACTG 
GGCAGGCAAG AAGGTCCCGA GTCTGGAACA GGAACTCTGG GACTTGTTCC TGGAAAGAGT 
AAATTGAAAG ACAAGCTGTC TGCCATACGT GCTGAACGCC GCGAATCTCT TCAAAAGCAG 
GATGCCATCC ATGAAGTGGA TTCATCTGAA GATGAGACAG ACGAAGGGTC GGAAGACAGC 
CAAGATGGGC GCAGAACTTG CTATGCTCCT CCTAGCCATG TCTTGAGGCC TACAACTGTA 
ATGGCCTCTC CCCATACCAT CAGAATGGGG CCAGCGGCTC CACCAACACT GGGACTTTTT 
CCTTCAACAG TTGCACCGCC ATGCTCACCT CAGCTCATCA GAGGACTGCA GATACAAAAC 
CAAAGTACCA CTCAATCTCA GACACAAACT TCAACCTCAC AACCAGCGCA AGCTGCAGTT 
AAACCCCCAG AACAGAAACC AATAACCTCT GTTAAAGACT CAAGTTCAAC GATAATTGAT 
GATTCGAAAA AACAGTCACA GGCACAAGAC ATTCAGTCCA GACCTTCTCC ACATCAACCC 
AGTCCTCTGT CTAGTACCTT TTCCACCCAA GGCAGCGCCT TTACCTCTGT TAGTAAAGAC 
CCATTTGTCA AGCCCACCAC ACCTCCATTG GATCCCCGGA GGACCCCAAA AATGTCTGAG 
CCAAGGTCAA AAACCAGTGG TAGAACTGAT TCTAGCACTA CTTCTGGAGT AGCCAGGGAT 
ACCCCAGTTG CGACTACCCC AACAGGAGGA ATCACTAAGG AGATGAAGGA GGCTGACAAT 
CGCAGACAGG CTGCAGCTGC TGCTGCTGCC TCTGCCGCCG CTGCTACCAC CTCTGCATCC 
ACTGCCTCTG AAAGTGGAAT GGATAAAGTT GTGTCTCAGC TCGCCACTGT GGCCAAGAGT 
GTGCTTGGAC CTGTCAAGCT CAATATCCCA GGTACTAAAG AGACCATGGA ACGAACAAAG 
GACCAGCGTA CCCAGGCAGA TGCCACCCAC CCAAAGATAA AAGAATGTCG CCTGGAGCAC 
CCTGATTCTC CTGACCAATT GAGTCCATCT GCTGCTTCGG GCTCCCAGTC TCCACGACTG 
ACTGAATCTC CATCTAAACA AACATCATCC AAATCTGAGC CAGCCTCAAC TTCTCAGAGA 
CCTTTAGAGG TCCCAGGGAC TTTCAAAAGA CAAACTTCTC CAATCCCTCA AGCTAGGGAA 
GAGCATCAGG ATAGGTCAGG AACTCTGCAA AGTGGCACCA CATCAAGGCC TAGGTCTGAA 
GCACAATCAC AGACCCAGCA GGCAAAGCCC AGTTCCACTA CGTCACGAGA TAAATCCAAC 
AAGACAACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	mast2
Entrez Gene ID	100001267
Full Name	microtubule associated serine/threonine kinase 2
Synonyms	im:7150634
General protein information	Preferred Names microtubule associated serine/threonine kinase 2 Names microtubule-associated serine/threonine-protein kinase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Canis lupus familiaris (dog)	MAST2	XP_005629101.1
Mus musculus (house mouse)	Mast2	NP_001036208.1
Danio rerio (zebrafish)	mast2	XP_001341305.4
Pan troglodytes (chimpanzee)	MAST2	XP_513151.3
Rattus norvegicus (Norway rat)	Mast2	NP_001101475.1
Bos taurus (cattle)	MAST2	XP_005204937.1
Gallus gallus (chicken)	MAST2	XP_004936815.1
Homo sapiens (human)	MAST2	NP_055927.2
	MAST2	XP_001105315.1
Xenopus tropicalis (tropical clawed frog)	mast2	NP_001122121.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

mast2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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