NGLY1 cDNA ORF clone, Bos taurus(cattle)

The following NGLY1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NGLY1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa231370 XM_024986293.1
Latest version!
Bos taurus N-glycanase 1 (NGLY1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa37752 NM_001192787.1
Latest version!
Bos taurus N-glycanase 1 (NGLY1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OBa34065 XM_005226174.2
Latest version!
Bos taurus N-glycanase 1 (NGLY1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa231370
    Clone ID Related Accession (Same CDS sequence) XM_024986293.1
    Accession Version XM_024986293.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGCGG CAGCGGCGGC GGCCCTGGGC GGCTCTGCCG GGTCCGCGTC CCCGGCCGTG 
    GCCGAGCTCT GTCAGAACAC CCCGGAGACG TTCCTGGAGG CGTCCAGGTT GCTGCTCACC
    TACGCGGACA ACATCCTTAG AAACCCTAAT GATGAAAAAT ATAGATCTAT CCGGATTGGA
    AACACAACTT TTTCTACTCG ACTCTTGCCT GTCAGAGGAG CTGTTGAATG TTTATTCGAA
    ATGGGATTTG AAGAGGGAGA AACACATCTT ATCTTTCCTA AAAAAGCTTC AGTGGAGCAG
    CTGCAAAAAA TCCGTGACCT GATTGCCATA GAGAGAAGTA GCAGATTGGA TGGATCAGAG
    AAGAGCCATA AAACAGAATT ATCTCAGCAC CTTGCAGCCA GCTCCCAGCT TCCTACAGCA
    CCGTCTTCAC ACCCTGATGG GGTGACCCAG CCTCCAGGGA ACAGTGAAGG GCAGGCATCC
    AGACCCCCCG TGGCTCCAAT GGTTGCTGAC AATTCAGTCA TTCTAAAAGT TCTTCGGTCC
    AACATTCAGC ATGTACTGGT ATATGAGAAC CTTGCTCTCC AGGAGAAAGC ACTGGCTTGT
    ATTCCAGTCC AAGAACTGAA AAGGAGATCA CAAGAAAAGT TGTCTAGAGC CAGAAAATTG
    GATAAAGGTA CTAATGTAAG TGAAGAGGAT TTTCTTTTGC TGGAGCTTTT ACACTGGTTT
    AAGGAAGAAT TTTTTCACTG GGTAAACGAC ATTGTGTGCA GTAAGTGTGG TGGCCAGACT
    AAGTCCAGAG GCGAGTCATT ACTCCCCAGT GAAGAGGAGC TGAAGTGGGG TGCGAACAGA
    GTAGAAGACC ACTACTGTGA CACATGCCAG CTCAGCAACC GATTCCCACG GTATAATAAT
    CCTGAGAAGC TTCTGGAGAC GAGACGTGGA CGGTGCGGGG AGTGGGCCAA CTGCTTCACG
    CTGTGCTGCC GAGCCCTGGG CTTTGAAGCC CGCTACGTCT GGGATTACAC AGACCATGTC
    TGGACGGAGG TGTACTCACC ATCACAGCAG CGCTGGCTGC ACTGCGACTC GTGCGAGGAC
    GTCTGTGACA AGCCCCTGCT CTACGAGGTC GGCTGGGGCA AGAAGCTCTC CTACATCATA
    GCATTCTCTA AGGACGAGGT GGTTGATGTC ACTTGGCGAT ATTCTTGTAA ACATGAAGAG
    GTAATCTCTA GAAGAACTGA AATTAAAGAG GAAGTACTTC GAGAAACTAT TAATGGACTT
    AATAAGCAGA GGCAAGTATC TTTGTCAGAA AACAGAAAGA AAGAACTTCT CCAGAGGATT
    GTTGTGGAAC TTGTTGAATT CATCTCTCCC AGAAGCCCTC AGCCTGGAGA GCTTGGAGGG
    AGGACGTCTG GGTCCTTGGC CTGGAGACTC GCCCGAGGCG AAGCGGGCCC AGAGGTGTAT
    TTAGCCCGCA AGGAAGGATC ATCTTACGCT TACATTTCCT GGAAGTTTGA ATGTGGGTCA
    GTTGGCCTAA AAGTAGATAG TATTTCCATT CGGACAAGTA GTCAGACCTT TGAGACCGGG
    ACAGTACAGT GGAAACTGAG ATCTGATACC GCACAAGCGG AACTGACAGG CGATAAAACA
    CTTCGCTCCT ATCACGACTT TTCTGGGGCC ACTGAAGTTA CTCTGGAAGC AAAATTAAGC
    AGAGGAGATG GTGTTGTTGC TTGGCAACAC ACCCAGCTGT TTAGACAAAG CTTAAACGAC
    CATGAAGAAA ATTGTTTGGA GATAATTATA AAATTCAGTG ACCTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842061.1
    CDS24..1811
    Translation

    Target ORF information:

    RefSeq Version XM_024986293.1
    Organism Bos taurus(cattle)
    Definition Bos taurus N-glycanase 1 (NGLY1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986293.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGCGG CAGCGGCGGC GGCCCTGGGC GGCTCTGCCG GGTCCGCGTC CCCGGCCGTG 
    GCCGAGCTCT GTCAGAACAC CCCGGAGACG TTCCTGGAGG CGTCCAGGTT GCTGCTCACC
    TACGCGGACA ACATCCTTAG AAACCCTAAT GATGAAAAAT ATAGATCTAT CCGGATTGGA
    AACACAACTT TTTCTACTCG ACTCTTGCCT GTCAGAGGAG CTGTTGAATG TTTATTCGAA
    ATGGGATTTG AAGAGGGAGA AACACATCTT ATCTTTCCTA AAAAAGCTTC AGTGGAGCAG
    CTGCAAAAAA TCCGTGACCT GATTGCCATA GAGAGAAGTA GCAGATTGGA TGGATCAGAG
    AAGAGCCATA AAACAGAATT ATCTCAGCAC CTTGCAGCCA GCTCCCAGCT TCCTACAGCA
    CCGTCTTCAC ACCCTGATGG GGTGACCCAG CCTCCAGGGA ACAGTGAAGG GCAGGCATCC
    AGACCCCCCG TGGCTCCAAT GGTTGCTGAC AATTCAGTCA TTCTAAAAGT TCTTCGGTCC
    AACATTCAGC ATGTACTGGT ATATGAGAAC CTTGCTCTCC AGGAGAAAGC ACTGGCTTGT
    ATTCCAGTCC AAGAACTGAA AAGGAGATCA CAAGAAAAGT TGTCTAGAGC CAGAAAATTG
    GATAAAGGTA CTAATGTAAG TGAAGAGGAT TTTCTTTTGC TGGAGCTTTT ACACTGGTTT
    AAGGAAGAAT TTTTTCACTG GGTAAACGAC ATTGTGTGCA GTAAGTGTGG TGGCCAGACT
    AAGTCCAGAG GCGAGTCATT ACTCCCCAGT GAAGAGGAGC TGAAGTGGGG TGCGAACAGA
    GTAGAAGACC ACTACTGTGA CACATGCCAG CTCAGCAACC GATTCCCACG GTATAATAAT
    CCTGAGAAGC TTCTGGAGAC GAGACGTGGA CGGTGCGGGG AGTGGGCCAA CTGCTTCACG
    CTGTGCTGCC GAGCCCTGGG CTTTGAAGCC CGCTACGTCT GGGATTACAC AGACCATGTC
    TGGACGGAGG TGTACTCACC ATCACAGCAG CGCTGGCTGC ACTGCGACTC GTGCGAGGAC
    GTCTGTGACA AGCCCCTGCT CTACGAGGTC GGCTGGGGCA AGAAGCTCTC CTACATCATA
    GCATTCTCTA AGGACGAGGT GGTTGATGTC ACTTGGCGAT ATTCTTGTAA ACATGAAGAG
    GTAATCTCTA GAAGAACTGA AATTAAAGAG GAAGTACTTC GAGAAACTAT TAATGGACTT
    AATAAGCAGA GGCAAGTATC TTTGTCAGAA AACAGAAAGA AAGAACTTCT CCAGAGGATT
    GTTGTGGAAC TTGTTGAATT CATCTCTCCC AGAAGCCCTC AGCCTGGAGA GCTTGGAGGG
    AGGACGTCTG GGTCCTTGGC CTGGAGACTC GCCCGAGGCG AAGCGGGCCC AGAGGTGTAT
    TTAGCCCGCA AGGAAGGATC ATCTTACGCT TACATTTCCT GGAAGTTTGA ATGTGGGTCA
    GTTGGCCTAA AAGTAGATAG TATTTCCATT CGGACAAGTA GTCAGACCTT TGAGACCGGG
    ACAGTACAGT GGAAACTGAG ATCTGATACC GCACAAGCGG AACTGACAGG CGATAAAACA
    CTTCGCTCCT ATCACGACTT TTCTGGGGCC ACTGAAGTTA CTCTGGAAGC AAAATTAAGC
    AGAGGAGATG GTGTTGTTGC TTGGCAACAC ACCCAGCTGT TTAGACAAAG CTTAAACGAC
    CATGAAGAAA ATTGTTTGGA GATAATTATA AAATTCAGTG ACCTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37752
    Clone ID Related Accession (Same CDS sequence) NM_001192787.1
    Accession Version NM_001192787.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000027.1. On Jul 17, 2010 this sequence version replaced XM_613238.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571262.9156.1, SRR5571254.45295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCGGCGG CAGCGGCGGC GGCCCTGGGC GGCTCTGCCG GGTCCGCGTC CCCGGCCGTG 
    GCCGAGCTCT GTCAGAACAC CCCGGAGACG TTCCTGGAGG CGTCCAGGTT GCTGCTCACC
    TACGCGGACA ACATCCTTAG AAACCCTAAT GATGAAAAAT ATAGATCTAT CCGGATTGGA
    AACACAACTT TTTCTACTCG ACTCTTGCCT GTCAGAGGAG CTGTTGAATG TTTATTCGAA
    ATGGGATTTG AAGAGGGAGA AACACATCTT ATCTTTCCTA AAAAAGCTTC AGTGGAGCAG
    CTGCAAAAAA TCCGTGACCT GATTGCCATA GAGAGAAGTA GCAGATTGGA TGGATCAGAG
    AAGAGCCATA AAACAGAATT ATCTCAGCAC CTTGCAGCCA GCTCCCAGCT TCCTACAGCA
    CCGTCTTCAC ACCCTGATGG GGTGACCCAG CCTCCAGGGA ACAGTGAAGG GCAGGCATCC
    AGACCCCCCG TGGCTCCAAT GGTTGCTGAC AATTCAGTCA TTCTAAAAGT TCTTCGGTCC
    AACATTCAGC ATGTACTGGT ATATGAGAAC CTTGCTCTCC AGGAGAAAGC ACTGGCTTGT
    ATTCCAGTCC AAGAACTGAA AAGGAGATCA CAAGAAAAGT TGTCTAGAGC CAGAAAATTG
    GATAAAGGTA CTAATGTAAG TGAAGAGGAT TTTCTTTTGC TGGAGCTTTT ACACTGGTTT
    AAGGAAGAAT TTTTTCACTG GGTAAACGAC ATTGTGTGCA GTAAGTGTGG TGGCCAGACT
    AAGTCCAGAG GCGAGTCATT ACTCCCCAGT GAAGAGGAGC TGAAGTGGGG TGCGAACAGA
    GTAGAAGACC ACTACTGTGA CACATGCCAG CTCAGCAACC GATTCCCACG GTATAATAAT
    CCTGAGAAGC TTCTGGAGAC GAGACGTGGA CGGTGCGGGG AGTGGGCCAA CTGCTTCACG
    CTGTGCTGCC GAGCCCTGGG CTTTGAAGCC CGCTACGTCT GGGATTACAC AGACCATGTC
    TGGACGGAGG TGTACTCACC ATCACAGCAG CGCTGGCTGC ACTGCGACTC GTGCGAGGAC
    GTCTGTGACA AGCCCCTGCT CTACGAGGTC GGCTGGGGCA AGAAGCTCTC CTACATCATA
    GCATTCTCTA AGGACGAGGT GGTTGATGTC ACTTGGCGAT ATTCTTGTAA ACATGAAGAG
    GTAATCTCTA GAAGAACTGA AATTAAAGAG GAAGTACTTC GAGAAACTAT TAATGGACTT
    AATAAGCAGA GGCAAGTATC TTTGTCAGAA AACAGAAAGA AAGAACTTCT CCAGAGGATT
    GTTGTGGAAC TTGTTGAATT CATCTCTCCC AGAAGCCCTC AGCCTGGAGA GCTTGGAGGG
    AGGACGTCTG GGTCCTTGGC CTGGAGACTC GCCCGAGGCG AAGCGGGCCC AGAGAGTAAA
    GAAACCCTGT TCATCCCCTC TGAAAATGAG AAGATTTCTA AACAGCTCCA CCTTTGCTAT
    AACATTGTAA AAAATTATTA TGTCCGAATT TCAAGTAATA ACCAAACCAT TTCTGGATGG
    GAGAATGGTG TGTGGAGAAT GGAATCCATA TTCAGAAAAG TTGAAACAGA CTGGAACATG
    GTGTATTTAG CCCGCAAGGA AGGATCATCT TACGCTTACA TTTCCTGGAA GTTTGAATGT
    GGGTCAGTTG GCCTAAAAGT AGATAGTATT TCCATTCGGA CAAGTAGTCA GACCTTTGAG
    ACCGGGACAG TACAGTGGAA ACTGAGATCT GATACCGCAC AAGCGGAACT GACAGGCGAT
    AAAACACTTC GCTCCTATCA CGACTTTTCT GGGGCCACTG AAGTTACTCT GGAAGCAAAA
    TTAAGCAGAG GAGATGGTGT TGTTGCTTGG CAACACACCC AGCTGTTTAG ACAAAGCTTA
    AACGACCATG AAGAAAATTG TTTGGAGATA ATTATAAAAT TCAGTGACCT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179716.1
    CDS24..1997
    Translation

    Target ORF information:

    RefSeq Version NM_001192787.1
    Organism Bos taurus(cattle)
    Definition Bos taurus N-glycanase 1 (NGLY1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192787.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCGGCGG CAGCGGCGGC GGCCCTGGGC GGCTCTGCCG GGTCCGCGTC CCCGGCCGTG 
    GCCGAGCTCT GTCAGAACAC CCCGGAGACG TTCCTGGAGG CGTCCAGGTT GCTGCTCACC
    TACGCGGACA ACATCCTTAG AAACCCTAAT GATGAAAAAT ATAGATCTAT CCGGATTGGA
    AACACAACTT TTTCTACTCG ACTCTTGCCT GTCAGAGGAG CTGTTGAATG TTTATTCGAA
    ATGGGATTTG AAGAGGGAGA AACACATCTT ATCTTTCCTA AAAAAGCTTC AGTGGAGCAG
    CTGCAAAAAA TCCGTGACCT GATTGCCATA GAGAGAAGTA GCAGATTGGA TGGATCAGAG
    AAGAGCCATA AAACAGAATT ATCTCAGCAC CTTGCAGCCA GCTCCCAGCT TCCTACAGCA
    CCGTCTTCAC ACCCTGATGG GGTGACCCAG CCTCCAGGGA ACAGTGAAGG GCAGGCATCC
    AGACCCCCCG TGGCTCCAAT GGTTGCTGAC AATTCAGTCA TTCTAAAAGT TCTTCGGTCC
    AACATTCAGC ATGTACTGGT ATATGAGAAC CTTGCTCTCC AGGAGAAAGC ACTGGCTTGT
    ATTCCAGTCC AAGAACTGAA AAGGAGATCA CAAGAAAAGT TGTCTAGAGC CAGAAAATTG
    GATAAAGGTA CTAATGTAAG TGAAGAGGAT TTTCTTTTGC TGGAGCTTTT ACACTGGTTT
    AAGGAAGAAT TTTTTCACTG GGTAAACGAC ATTGTGTGCA GTAAGTGTGG TGGCCAGACT
    AAGTCCAGAG GCGAGTCATT ACTCCCCAGT GAAGAGGAGC TGAAGTGGGG TGCGAACAGA
    GTAGAAGACC ACTACTGTGA CACATGCCAG CTCAGCAACC GATTCCCACG GTATAATAAT
    CCTGAGAAGC TTCTGGAGAC GAGACGTGGA CGGTGCGGGG AGTGGGCCAA CTGCTTCACG
    CTGTGCTGCC GAGCCCTGGG CTTTGAAGCC CGCTACGTCT GGGATTACAC AGACCATGTC
    TGGACGGAGG TGTACTCACC ATCACAGCAG CGCTGGCTGC ACTGCGACTC GTGCGAGGAC
    GTCTGTGACA AGCCCCTGCT CTACGAGGTC GGCTGGGGCA AGAAGCTCTC CTACATCATA
    GCATTCTCTA AGGACGAGGT GGTTGATGTC ACTTGGCGAT ATTCTTGTAA ACATGAAGAG
    GTAATCTCTA GAAGAACTGA AATTAAAGAG GAAGTACTTC GAGAAACTAT TAATGGACTT
    AATAAGCAGA GGCAAGTATC TTTGTCAGAA AACAGAAAGA AAGAACTTCT CCAGAGGATT
    GTTGTGGAAC TTGTTGAATT CATCTCTCCC AGAAGCCCTC AGCCTGGAGA GCTTGGAGGG
    AGGACGTCTG GGTCCTTGGC CTGGAGACTC GCCCGAGGCG AAGCGGGCCC AGAGAGTAAA
    GAAACCCTGT TCATCCCCTC TGAAAATGAG AAGATTTCTA AACAGCTCCA CCTTTGCTAT
    AACATTGTAA AAAATTATTA TGTCCGAATT TCAAGTAATA ACCAAACCAT TTCTGGATGG
    GAGAATGGTG TGTGGAGAAT GGAATCCATA TTCAGAAAAG TTGAAACAGA CTGGAACATG
    GTGTATTTAG CCCGCAAGGA AGGATCATCT TACGCTTACA TTTCCTGGAA GTTTGAATGT
    GGGTCAGTTG GCCTAAAAGT AGATAGTATT TCCATTCGGA CAAGTAGTCA GACCTTTGAG
    ACCGGGACAG TACAGTGGAA ACTGAGATCT GATACCGCAC AAGCGGAACT GACAGGCGAT
    AAAACACTTC GCTCCTATCA CGACTTTTCT GGGGCCACTG AAGTTACTCT GGAAGCAAAA
    TTAAGCAGAG GAGATGGTGT TGTTGCTTGG CAACACACCC AGCTGTTTAG ACAAAGCTTA
    AACGACCATG AAGAAAATTG TTTGGAGATA ATTATAAAAT TCAGTGACCT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34065
    Clone ID Related Accession (Same CDS sequence) XM_005226174.2
    Accession Version XM_005226174.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005226174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGGCGG CAGCGGCGGC GGCCCTGGGC GGCTCTGCCG GGTCCGCGTC CCCGGCCGTG 
    GCCGAGCTCT GTCAGAACAC CCCGGAGACG TTCCTGGAGG CGTCCAGGTT GCTGCTCACC
    TACGCGGACA ACATCCTTAG AAACCCTAAT GATGAAAAAT ATAGATCTAT CCGGATTGGA
    AACACAACTT TTTCTACTCG ACTCTTGCCT GTCAGAGGAG CTGTTGAATG TTTATTCGAA
    ATGGGATTTG AAGAGGGAGA AACACATCTT ATCTTTCCTA AAAAAGCTTC AGTGGAGCAG
    CTGCAAAAAA TCCGTGACCT GATTGCCATA GAGAGAAGTA GCAGATTGGA TGGATCAGAG
    AAGAGCCATA AAACAGAATT ATCTCAGCAC CTTGCAGCCA GCTCCCAGCT TCCTACAGCA
    CCGTCTTCAC ACCCTGATGG GGTGACCCAG CCTCCAGGGA ACAGTGAAGG GCAGGCATCC
    AGACCCCCCG TGGCTCCAAT GGTTGCTGAC AATTCAGTCA TTCTAAAAGT TCTTCGGTCC
    AACATTCAGC ATGTACTGGT ATATGAGAAC CTTGCTCTCC AGGAGAAAGC ACTGGCTTGT
    ATTCCAGTCC AAGAACTGAA AAGGAGATCA CAAGAAAAGT TGTCTAGAGC CAGAAAATTG
    GATAAAGGTA CTAATGTAAG TGAAGAGGAT TTTCTTTTGC TGGAGCTTTT ACACTGGTTT
    AAGGAAGAAT TTTTTCACTG GGTAAACGAC ATTGTGTGCA GTAAGTGTGG TGGCCAGACT
    AAGTCCAGAG GCGAGTCATT ACTCCCCAGT GAAGAGGAGC TGAAGTGGGG TGCGAACAGA
    GTAGAAGACC ACTACTGTGA CACATGCCAG CTCAGCAACC GATTCCCACG GTATAATAAT
    CCTGAGAAGC TTCTGGAGAC GAGACGTGGA CGGTGCGGGG AGTGGGCCAA CTGCTTCACG
    CTGTGCTGCC GAGCCCTGGG CTTTGAAGCC CGCTACGTCT GGGATTACAC AGACCATGTC
    TGGACGGAGG TGTACTCACC ATCACAGCAG CGCTGGCTGC ACTGCGACTC GTGCGAGGAC
    GTCTGTGACA AGCCCCTGCT CTACGAGGTC GGCTGGGGCA AGAAGCTCTC CTACATCATA
    GCATTCTCTA AGGACGAGGT GGTTGATGTC ACTTGGCGAT ATTCTTGTAA ACATGAAGAG
    GTAATCTCTA GAAGAACTGA AATTAAAGAG GAAGTACTTC GAGAAACTAT TAATGGACTT
    AATAAGCAGA GGCAAGTATC TTTGTCAGAA AACAGAAAGA AAGAACTTCT CCAGAGGATT
    GTTGTGGAAC TTGTTGAATT CATCTCTCCC AGAAGCCCTC AGCCTGGAGA GCTTGGAGGG
    AGGACGTCTG GGTCCTTGGC CTGGAGACTC GCCCGAGGCG AAGCGGGCCC AGAGAGTAAA
    GAAACCCTGT TCATCCCCTC TGAAAATGAG AAGATTTCTA AACAGCTCCA CCTTTGCTAT
    AACATTGTAA AAAATTATTA TGTCCGAATT TCAAGTAATA ACCAAACCAT TTCTGGATGG
    GAGAATGGTG TGTGGAGAAT GGAATCCATA TTCAGAAAAG TTGAAACAGA CTGGAACATG
    ATAAAACACT TCGCTCCTAT CACGACTTTT CTGGGGCCAC TGAAGTTACT CTGGAAGCAA
    AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226231.1
    CDS24..1709
    Translation

    Target ORF information:

    RefSeq Version XM_005226174.2
    Organism Bos taurus(cattle)
    Definition Bos taurus N-glycanase 1 (NGLY1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226174.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGGCGG CAGCGGCGGC GGCCCTGGGC GGCTCTGCCG GGTCCGCGTC CCCGGCCGTG 
    GCCGAGCTCT GTCAGAACAC CCCGGAGACG TTCCTGGAGG CGTCCAGGTT GCTGCTCACC
    TACGCGGACA ACATCCTTAG AAACCCTAAT GATGAAAAAT ATAGATCTAT CCGGATTGGA
    AACACAACTT TTTCTACTCG ACTCTTGCCT GTCAGAGGAG CTGTTGAATG TTTATTCGAA
    ATGGGATTTG AAGAGGGAGA AACACATCTT ATCTTTCCTA AAAAAGCTTC AGTGGAGCAG
    CTGCAAAAAA TCCGTGACCT GATTGCCATA GAGAGAAGTA GCAGATTGGA TGGATCAGAG
    AAGAGCCATA AAACAGAATT ATCTCAGCAC CTTGCAGCCA GCTCCCAGCT TCCTACAGCA
    CCGTCTTCAC ACCCTGATGG GGTGACCCAG CCTCCAGGGA ACAGTGAAGG GCAGGCATCC
    AGACCCCCCG TGGCTCCAAT GGTTGCTGAC AATTCAGTCA TTCTAAAAGT TCTTCGGTCC
    AACATTCAGC ATGTACTGGT ATATGAGAAC CTTGCTCTCC AGGAGAAAGC ACTGGCTTGT
    ATTCCAGTCC AAGAACTGAA AAGGAGATCA CAAGAAAAGT TGTCTAGAGC CAGAAAATTG
    GATAAAGGTA CTAATGTAAG TGAAGAGGAT TTTCTTTTGC TGGAGCTTTT ACACTGGTTT
    AAGGAAGAAT TTTTTCACTG GGTAAACGAC ATTGTGTGCA GTAAGTGTGG TGGCCAGACT
    AAGTCCAGAG GCGAGTCATT ACTCCCCAGT GAAGAGGAGC TGAAGTGGGG TGCGAACAGA
    GTAGAAGACC ACTACTGTGA CACATGCCAG CTCAGCAACC GATTCCCACG GTATAATAAT
    CCTGAGAAGC TTCTGGAGAC GAGACGTGGA CGGTGCGGGG AGTGGGCCAA CTGCTTCACG
    CTGTGCTGCC GAGCCCTGGG CTTTGAAGCC CGCTACGTCT GGGATTACAC AGACCATGTC
    TGGACGGAGG TGTACTCACC ATCACAGCAG CGCTGGCTGC ACTGCGACTC GTGCGAGGAC
    GTCTGTGACA AGCCCCTGCT CTACGAGGTC GGCTGGGGCA AGAAGCTCTC CTACATCATA
    GCATTCTCTA AGGACGAGGT GGTTGATGTC ACTTGGCGAT ATTCTTGTAA ACATGAAGAG
    GTAATCTCTA GAAGAACTGA AATTAAAGAG GAAGTACTTC GAGAAACTAT TAATGGACTT
    AATAAGCAGA GGCAAGTATC TTTGTCAGAA AACAGAAAGA AAGAACTTCT CCAGAGGATT
    GTTGTGGAAC TTGTTGAATT CATCTCTCCC AGAAGCCCTC AGCCTGGAGA GCTTGGAGGG
    AGGACGTCTG GGTCCTTGGC CTGGAGACTC GCCCGAGGCG AAGCGGGCCC AGAGAGTAAA
    GAAACCCTGT TCATCCCCTC TGAAAATGAG AAGATTTCTA AACAGCTCCA CCTTTGCTAT
    AACATTGTAA AAAATTATTA TGTCCGAATT TCAAGTAATA ACCAAACCAT TTCTGGATGG
    GAGAATGGTG TGTGGAGAAT GGAATCCATA TTCAGAAAAG TTGAAACAGA CTGGAACATG
    ATAAAACACT TCGCTCCTAT CACGACTTTT CTGGGGCCAC TGAAGTTACT CTGGAAGCAA
    AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.